LOCUS       DACIRY010000001        54254 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.001, whole genome shotgun sequence.
ACCESSION   DACIRY010000001 DACIRY010000000
VERSION     DACIRY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.001"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IYE22_000001"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IYE22_000001"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ9505256.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IYE22_000002"
     CDS             complement(937..1518)
                     /locus_tag="IYE22_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9505257.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IYE22_000003"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IYE22_000003"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ9505258.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IYE22_000004"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IYE22_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ9505259.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IYE22_000005"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IYE22_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ9505260.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IYE22_000006"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IYE22_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ9505261.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IYE22_000007"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IYE22_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ9505262.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IYE22_000008"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IYE22_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ9505263.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IYE22_000009"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IYE22_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ9505264.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IYE22_000010"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IYE22_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ9505265.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IYE22_000011"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IYE22_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ9505266.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IYE22_000012"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IYE22_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ9505267.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IYE22_000013"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IYE22_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ9505268.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IYE22_000014"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IYE22_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ9505269.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IYE22_000015"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IYE22_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ9505270.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IYE22_000016"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IYE22_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ9505271.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IYE22_000017"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IYE22_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ9505272.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IYE22_000018"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IYE22_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ9505273.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IYE22_000019"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IYE22_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ9505274.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IYE22_000020"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IYE22_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ9505275.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IYE22_000021"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IYE22_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ9505276.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IYE22_000022"
     CDS             13972..14157
                     /locus_tag="IYE22_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ9505277.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IYE22_000023"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IYE22_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ9505278.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IYE22_000024"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IYE22_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ9505279.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IYE22_000025"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IYE22_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ9505280.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IYE22_000026"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IYE22_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ9505281.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IYE22_000027"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IYE22_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ9505282.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IYE22_000028"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IYE22_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ9505283.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IYE22_000029"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IYE22_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ9505284.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IYE22_000030"
     CDS             18232..18879
                     /locus_tag="IYE22_000030"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ9505285.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IYE22_000031"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IYE22_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ9505286.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IYE22_000032"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IYE22_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ9505287.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IYE22_000033"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IYE22_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ9505288.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IYE22_000034"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IYE22_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ9505289.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IYE22_000035"
     CDS             20317..21255
                     /locus_tag="IYE22_000035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ9505290.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IYE22_000036"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IYE22_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ9505291.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IYE22_000037"
     CDS             21935..22774
                     /locus_tag="IYE22_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ9505292.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IYE22_000038"
     CDS             22750..23619
                     /locus_tag="IYE22_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ9505293.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IYE22_000039"
     CDS             23609..24406
                     /locus_tag="IYE22_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ9505294.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IYE22_000040"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IYE22_000040"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ9505295.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IYE22_000041"
     CDS             complement(25688..28357)
                     /locus_tag="IYE22_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ9505296.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IYE22_000042"
     CDS             complement(28595..28783)
                     /locus_tag="IYE22_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ9505297.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IYE22_000043"
     CDS             29053..29415
                     /locus_tag="IYE22_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ9505298.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IYE22_000044"
     CDS             29467..31149
                     /locus_tag="IYE22_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ9505299.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IYE22_000045"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IYE22_000045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ9505300.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IYE22_000046"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IYE22_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ9505301.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IYE22_000047"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IYE22_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ9505302.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IYE22_000048"
     CDS             34984..35988
                     /locus_tag="IYE22_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505303.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IYE22_000049"
     CDS             35989..36933
                     /locus_tag="IYE22_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505304.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IYE22_000050"
     CDS             36933..37895
                     /locus_tag="IYE22_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9505305.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IYE22_000051"
     CDS             37922..38836
                     /locus_tag="IYE22_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9505306.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IYE22_000052"
     CDS             38862..40643
                     /locus_tag="IYE22_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9505307.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IYE22_000053"
     CDS             40991..41677
                     /locus_tag="IYE22_000053"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ9505308.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IYE22_000054"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IYE22_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ9505309.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IYE22_000055"
     CDS             45287..46096
                     /locus_tag="IYE22_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9505310.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IYE22_000056"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IYE22_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ9505311.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IYE22_000057"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IYE22_000057"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ9505312.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="IYE22_000058"
     CDS             complement(49648..50100)
                     /locus_tag="IYE22_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ9505313.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="IYE22_000059"
     CDS             50250..51200
                     /locus_tag="IYE22_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ9505314.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="IYE22_000060"
     CDS             51193..52152
                     /locus_tag="IYE22_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ9505315.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="IYE22_000061"
     CDS             52149..52904
                     /locus_tag="IYE22_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9505316.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSTLRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="IYE22_000062"
     CDS             52927..53880
                     /locus_tag="IYE22_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9505317.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        17803 a         9833 c        11685 g        14933 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgaccctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taag
//
LOCUS       DACIRY010000002        53198 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.002, whole genome shotgun sequence.
ACCESSION   DACIRY010000002 DACIRY010000000
VERSION     DACIRY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.002"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            6..965
                     /locus_tag="IYE22_000063"
                     /pseudo
     CDS             6..965
                     /locus_tag="IYE22_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1496..1906
                     /locus_tag="IYE22_000064"
     CDS             1496..1906
                     /locus_tag="IYE22_000064"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ9505318.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            1899..2354
                     /locus_tag="IYE22_000065"
     CDS             1899..2354
                     /locus_tag="IYE22_000065"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9505319.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            2371..3102
                     /locus_tag="IYE22_000066"
     CDS             2371..3102
                     /locus_tag="IYE22_000066"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9505320.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            3119..3931
                     /locus_tag="IYE22_000067"
     CDS             3119..3931
                     /locus_tag="IYE22_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9505321.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(4147..5430)
                     /locus_tag="IYE22_000068"
                     /pseudo
     CDS             complement(4147..5430)
                     /locus_tag="IYE22_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5505..6916)
                     /locus_tag="IYE22_000069"
     CDS             complement(join(5505..6361,6361..6916))
                     /locus_tag="IYE22_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9505322.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            complement(7005..7088)
                     /locus_tag="IYE22_000070"
                     /pseudo
     CDS             complement(7005..7088)
                     /locus_tag="IYE22_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7605..8855
                     /locus_tag="IYE22_000071"
                     /pseudo
     CDS             7605..8855
                     /locus_tag="IYE22_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(9429..10283)
                     /locus_tag="IYE22_000072"
     CDS             complement(9429..10283)
                     /locus_tag="IYE22_000072"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505323.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(10300..11298)
                     /locus_tag="IYE22_000073"
     CDS             complement(10300..11298)
                     /locus_tag="IYE22_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9505324.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            complement(11333..12157)
                     /locus_tag="IYE22_000074"
     CDS             complement(11333..12157)
                     /locus_tag="IYE22_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9505325.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            complement(12147..12929)
                     /locus_tag="IYE22_000075"
     CDS             complement(12147..12929)
                     /locus_tag="IYE22_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9505326.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            complement(12953..13423)
                     /locus_tag="IYE22_000076"
     CDS             complement(12953..13423)
                     /locus_tag="IYE22_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9505327.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            complement(13441..13863)
                     /locus_tag="IYE22_000077"
     CDS             complement(13441..13863)
                     /locus_tag="IYE22_000077"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAQ9505328.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            complement(13991..16714)
                     /locus_tag="IYE22_000078"
     CDS             complement(13991..16714)
                     /locus_tag="IYE22_000078"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9505329.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            16904..17920
                     /locus_tag="IYE22_000079"
     CDS             16904..17920
                     /locus_tag="IYE22_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9505330.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            18028..18897
                     /locus_tag="IYE22_000080"
     CDS             18028..18897
                     /locus_tag="IYE22_000080"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505331.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            18912..19616
                     /locus_tag="IYE22_000081"
     CDS             18912..19616
                     /locus_tag="IYE22_000081"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9505332.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            20837..21450
                     /locus_tag="IYE22_000082"
                     /pseudo
     CDS             20837..21450
                     /locus_tag="IYE22_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(22199..25348)
                     /locus_tag="IYE22_000083"
     CDS             complement(22199..25348)
                     /locus_tag="IYE22_000083"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ9505333.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(25361..26578)
                     /locus_tag="IYE22_000084"
     CDS             complement(25361..26578)
                     /locus_tag="IYE22_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ9505334.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(26568..28163)
                     /locus_tag="IYE22_000085"
     CDS             complement(26568..28163)
                     /locus_tag="IYE22_000085"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ9505335.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(28673..29455)
                     /locus_tag="IYE22_000086"
     CDS             complement(28673..29455)
                     /locus_tag="IYE22_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505336.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(29520..30356)
                     /locus_tag="IYE22_000087"
     CDS             complement(29520..30356)
                     /locus_tag="IYE22_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9505337.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(30374..31783)
                     /locus_tag="IYE22_000088"
     CDS             complement(30374..31783)
                     /locus_tag="IYE22_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9505338.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(31797..33620)
                     /locus_tag="IYE22_000089"
     CDS             complement(31797..33620)
                     /locus_tag="IYE22_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9505339.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(34463..34789)
                     /locus_tag="IYE22_000090"
     CDS             complement(34463..34789)
                     /locus_tag="IYE22_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505340.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(34835..35197)
                     /locus_tag="IYE22_000091"
     CDS             complement(34835..35197)
                     /locus_tag="IYE22_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ9505341.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(35234..35449)
                     /locus_tag="IYE22_000092"
     CDS             complement(35234..35449)
                     /locus_tag="IYE22_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9505342.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            35576..35794
                     /locus_tag="IYE22_000093"
     CDS             35576..35794
                     /locus_tag="IYE22_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9505343.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVEL"
     gene            complement(35988..37985)
                     /locus_tag="IYE22_000094"
     CDS             complement(35988..37985)
                     /locus_tag="IYE22_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505344.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(38083..38358)
                     /locus_tag="IYE22_000095"
     CDS             complement(38083..38358)
                     /locus_tag="IYE22_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9505345.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            38590..39324
                     /locus_tag="IYE22_000096"
     CDS             38590..39324
                     /locus_tag="IYE22_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9505346.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            39363..40505
                     /locus_tag="IYE22_000097"
     CDS             39363..40505
                     /locus_tag="IYE22_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9505347.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(40600..41991)
                     /gene="sufB"
                     /locus_tag="IYE22_000098"
     CDS             complement(40600..41991)
                     /gene="sufB"
                     /locus_tag="IYE22_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ9505348.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(41996..42466)
                     /locus_tag="IYE22_000099"
     CDS             complement(41996..42466)
                     /locus_tag="IYE22_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ9505349.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(42453..43688)
                     /locus_tag="IYE22_000100"
     CDS             complement(42453..43688)
                     /locus_tag="IYE22_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ9505350.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(43685..44971)
                     /gene="sufD"
                     /locus_tag="IYE22_000101"
     CDS             complement(43685..44971)
                     /gene="sufD"
                     /locus_tag="IYE22_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ9505351.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(44984..45757)
                     /gene="sufC"
                     /locus_tag="IYE22_000102"
     CDS             complement(44984..45757)
                     /gene="sufC"
                     /locus_tag="IYE22_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ9505352.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(45954..46802)
                     /locus_tag="IYE22_000103"
     CDS             complement(45954..46802)
                     /locus_tag="IYE22_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9505353.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(46826..47512)
                     /locus_tag="IYE22_000104"
     CDS             complement(46826..47512)
                     /locus_tag="IYE22_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9505354.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(47505..48539)
                     /locus_tag="IYE22_000105"
     CDS             complement(47505..48539)
                     /locus_tag="IYE22_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9505355.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(48934..49272)
                     /locus_tag="IYE22_000106"
     CDS             complement(48934..49272)
                     /locus_tag="IYE22_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ9505356.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(49274..49630)
                     /locus_tag="IYE22_000107"
     CDS             complement(49274..49630)
                     /locus_tag="IYE22_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ9505357.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(49636..50823)
                     /locus_tag="IYE22_000108"
     CDS             complement(49636..50823)
                     /locus_tag="IYE22_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ9505358.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(51014..51859)
                     /locus_tag="IYE22_000109"
     CDS             complement(51014..51859)
                     /locus_tag="IYE22_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9505359.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            52093..53058
                     /locus_tag="IYE22_000110"
     CDS             52093..53058
                     /locus_tag="IYE22_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ9505360.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        16104 a        10688 c         8877 g        17529 t
ORIGIN      
        1 gacaaatgac ctatacccat cttacaacaa atgaactcgt gatgatagaa gcctatttca
       61 atatgcatca atctgttacc gtagtcgcta agacctcaaa tcgttccaga cagaccatct
      121 acaatgtgta caacttcctg aaacaaggcc aatctatcca tgactattac caacaatatc
      181 aaaaaaataa aacacggtgc ggccgacgac ccatttgttt gccagcagaa caagatgcgt
      241 atatccgaaa caaggtggcg caaggatgga cacccgatgt gattatcggt cgtgctgagt
      301 tttcaatttc atgttctgtt cgaacccttt atcgcaagtt taaaacaaac taatttgatt
      361 gctcgaaact acccatgaaa ggaaaacgga aacccaacgg gcataaagaa cgtcgaggga
      421 aacaagcctt caaacggaat atttcagaac gtctcacgga ctatcctgaa tttcaacagg
      481 aatttggaca tttggaaggc gacactattg tgggtgtcca tcataaaagt gctgtcatca
      541 cgctggttga gcgcttgtca aaagtgatta ttactttaaa acccgaagga cggaaagcac
      601 tagatattga aaaagtgaca actgactggc tacaaaagct tccccgaaac ttgttcaaat
      661 ccattacctt tgattgcgga aaagaatttt cgaattggaa aaacatgagt aatgctaacg
      721 atatttctat ttattttgca gatcctggaa cgctgtcaca acggggatta aatgaacatt
      781 ctaatggatt gttacgtaaa gacggacttc ctaatacaat ggatttcaat caggttgacc
      841 aaaaatttgt ggcggcggtt gcctccaaaa gaaatcatat accaaggaaa tctttaaatt
      901 atcgaacatc gttagaagta tttttgagtt acataagtga ggagcaattg tctaacttaa
      961 tttgacattt agaaaataat aaaccatatc ccatctattc aaataatatt ttaccgattg
     1021 aaagacttgc taatcaaagc agaatagttt atatagataa attattttct ttaaacttat
     1081 actcattttt agatatttta taagaatact tcatgtcttc gccagtaata taaagaacat
     1141 ctgtctctcc atttggactt gggttatcta ttttgggtgt aacatactat acatacaacc
     1201 gatacataaa agatttgaca tctggacaaa acacaatgct ttacttcatt tttataggct
     1261 ttgtttacac catcacaaaa atcacctcct aatttacaaa ttcattaaac aaacagaaat
     1321 aaaaaatcac cacgatagcc gcgttcaaaa aaatttacaa aagagaattt tagttaaagt
     1381 ttttagattc gccccactaa ttaccaaagg tattttattt attcttaata cgaaataatt
     1441 tattgacatt tagattacca atggtatatt atttataaat aaatggaggt ggattatgta
     1501 cacaattatt cttgcaagcc acaaaaattt agcgaaagga atgaaagaga cagtcgaata
     1561 tatccttggc cctgcaccat tcataaaagt aatgactgca tacacagacg ataactatga
     1621 tattgaaaaa gatacgaata ttcttctcga tggaactgat aaagtccttg tcatcactga
     1681 tttacttggt ggtagcgtaa ataatcattg gatgaattat gtttacgaaa aaaaattaac
     1741 aaaaaaaatt acagtaattg caggtatgac tttgtcctta attatggaat taagtatgaa
     1801 cattgaagat tataaattga gagaaaaaat ttccatgatt attgctgaaa gccaaaagag
     1861 cattataaac tgttcagaat taatggaggt agaagataat gattaaacta ttaagaattg
     1921 acgatcgcct gatccacggt caagttgcaa tggcttggac taagtccgta tctgctgact
     1981 acattattgt agttgatgac gcctctgcta atgacaaaat gaaacaaatg attttatcat
     2041 tagcaaaacc tgtatcaacg ggactagata ttattacttc agcagatttt ttaagtacct
     2101 ttgaaaaaag gaaaaatcaa aatttgatga tagtcactag ttcccctaaa agcgcatttg
     2161 aaattgtaaa cgctatccca ggggaagtcg attcaattaa tatcggtggt ttacggtacg
     2221 ccgagggaaa agagaaagtt aacgagtatg tggctatcac atcagaagac aaaaactact
     2281 tagcaaaatt aaaggagtcc ggcttcaaat taattatgca ggctactcca actggaaaag
     2341 cgaagattta ttagaaggtg gggaataaga atgattgaat gcattttaat tggcatagtt
     2401 gggtatttaa cgacaataga tgaaagatat tttggggcat ctatgatgaa tcgaccgata
     2461 atagttggac ctgttgttgg acttatctta ggggatctcc atcaaggcat cttgatagga
     2521 gcagcattag aagcaatgtt tattggcatt gtaacgattg gtgctgcact accaccagat
     2581 gtaggtgtag ctggtactat tgcaacggct ctagcaataa aaagtggagc aggtgctgat
     2641 gtagctgtta cattggcgat gcctttcgct gtattggcac aaggattaaa catgttggct
     2701 tttacgatta actcctttac cgtttcacaa ggtagaaaag caattgaaaa ccgcaaatta
     2761 tcatcttttc agaagtggca tttttttcca ttaatcgttc gcttaccttc cgcagtactt
     2821 acatttttag tagtatattt tggtgccaat gctacagaaa gttttgtaaa tattatgcca
     2881 gaaaaattaa tgaccggttt caccgtagct tctggtttac tccccgctgt tggatttgcc
     2941 atgctaattc aaggaatgat tgacaaaaaa tcagttcctt attttttcat tggatacctt
     3001 ttagcggcat atttaggagt ccccgtagtt ggtgtagctg ttgctgcaat tatggttgct
     3061 ttattagtta tccaaactaa acgcaatcct gtaaatattt aaagggagag tgataattat
     3121 gggagattct aaattaacta aaaaagaaat aaacaaggtt ttctggcgtt catttgcttt
     3181 acagggatcc gagtcataca ctgatatgca aggaatggga tatagttttt ctatattacc
     3241 tgctttaaac aaaatatatc aagggaatga tgaaaaatat tatgaagctg tttaccgaaa
     3301 tacggaattt tttaatacaa cgcctcatat ttcaaccttt attatgggta tgtcattagc
     3361 acttgaagaa gaaaatgcaa aaacagatca atttgaaact gaaacaattt catcgataaa
     3421 agctgcattg atggggcctg tagctggtat tggtgatagt ttttactggg gaacatttcg
     3481 cgttattgct gctggcatcg gcttatcttt agctgcccaa ggaaacattt taggtccaat
     3541 attgtatttt tcaatttata cgctgttaca ttttcttggc agatatggct gcttacgtat
     3601 tggatatcgg atgggagcaa acgccattga aaaagcattt gagaacaatt accttgaaag
     3661 tttctcgtta gcagcatcta tcgtaggttt aatgtcaatt ggagccatga cctcatctct
     3721 ggtacgcttt actattcccg ccgtatttaa atcaaaaggt ggagaaataa atgttcagca
     3781 aatgctcgat gatatttttc caggaattct ctcattgggg ctaacattct ttgtttactt
     3841 tctattaaag aaaaaagtaa aggttctttg gattatactt gctatcttcg tctttagtat
     3901 tgttggagtt tatattggat tattaggtta attaatccag aataaaacta gatcagacat
     3961 caacttaaac cataaaatac ccaaagataa atgtaaatat gagtaaaaag agcatggtat
     4021 aaattattag gatctataat ttatgggtct gatgaatcag aattgattct gtagtgacct
     4081 aaaaaagata gatgttttcg gagtggagaa aattcactcc gtttttcttt gcttttcttg
     4141 atgattttag acagctttga atgctattac tctaaattgc accagactct gccagtttag
     4201 tttccttttc atacgctcat ggttgtagta atgaatatag ccatcaatgg cttgcttcaa
     4261 ttcttcaaag atacgatagt ctcttccata ataaatttcc tgctttaata gactaaggaa
     4321 gttctcaatg atcgaattat ctaagcaggt ctccttacgg gacatacttt ggaagatctt
     4381 atgttctttc aacttatgac tataagcttt catttggtag gtccatcctt tttggagtga
     4441 aaagttcgac gatagataca ttccgttgcg cagatagctt cctctaaagc ttccatgata
     4501 gccaacgcac ttggcttttc agaaatgcga taaaataaaa tttcactgtt gtacatatct
     4561 aaaaatggat caagatacag cttcttttca cgattggcac cgtatttatc catttcaaaa
     4621 tattttagtt ctgtcgtatc tgttgtaacc ttttgatgac agttgcttgt gtaaaagcgt
     4681 cgatgaattc gattttttgt aatcatgccg attcttccac gataagtatt atagcggcga
     4741 tattttctcg taaatgatcg tacttcaata ctcagctttc gaatcaaacg ttgtactttt
     4801 ttcttgttaa catggaaccc acggtcccac aattcctttg tcatacgtaa atagccataa
     4861 tctttatatt gacttcttat tttaagcatt tcctcttcga tttcttgatc tgaatttctt
     4921 cgatcaaatc gtctttgcca atacataaag gttaattcag gaaatttcaa ggtttcaaga
     4981 agctcaacta atttgaatga tcctcggagg ttgtcgatga ttcgtgcgat tgattcgttc
     5041 gtctttgtta gacttcctgt tttctaagct tcctcaattc ttttaaaaag gtattctcaa
     5101 tctgaaggaa acgcacttat ttttccaatt ctttgatacg attatgctct ttaggcgtca
     5161 gtttgcttgt ttcttccttt tattctttct tagacaaaat ggaggaggac cctttcattt
     5221 ttgagagccc gtgcaatcct tttgaacaaa aattttcatc caattgacaa ctaacgcagg
     5281 ttggtttatc cctaaatgat tcgccacttc acgataggat agctcacccg ttttttataa
     5341 ctctatcgca tctatcttga attgaacaga ataacttcgc ttatttcgct tacgcaagag
     5401 gccttcttct ccaaattctc gataagcatt tgtagcgacc ccaaaaagtt agacttttat
     5461 ggagtggaga aatccactcc ttttttgtac gataaaatta actgttagac agctgttttt
     5521 acagcttctc gaaactgaac cggacttcgc caatttaatt tctttttgat gcgtttatgg
     5581 ttgtaataat agatatattg gtcaattttt gttttcaatt catctaaact ccgatagact
     5641 tctccatgaa aaatctcttg ctttaataaa ccaaagaagt tttccatagg tgagttatct
     5701 aaacaattcc ctttacgaga catactctgg aagattttat gctgcttcaa tttattctta
     5761 taagcattca tttgataggc ccatccttga tctgaatgga aggttctacg aaatggacaa
     5821 tcatttgtca tctggattgc ttcttctaat ccttccatta tggctagtgc gtttggtttt
     5881 tcagaaatcc gataagatag aatttcacta ttgtagaaat ccatgaaagg atctaaatac
     5941 agctttttct gacgaacgac acctgtggtg tctacttcat aatacttaaa ctctgtggta
     6001 tcagttgtaa ttttttggtg acagatattt gtataaaacc gacgatgaat acgatttttg
     6061 gcgacagtgc caaccttccc atgataagaa ttgtaccgtc gagattttcg agtgtaagac
     6121 tttacttcga tacctagttt ttttatcaga cgttgaatct tctttttatt gacatgaatc
     6181 ccttgattct ctaattcttt ttttagtcgc agacagccat agtctttatg ctccttgcga
     6241 atttcaagca ttttttcttc aatttcttgg tctgggtttg gtctgtcaaa ccgtttctgc
     6301 caatacatat aggttgcttt aggaaatttc aatgtttcaa gaagctcaac tagtttgaat
     6361 gatcctcgga ggctggcgat gattcgtgcg attgattctt gcgtctttgc tgggcttcct
     6421 gttttcgcag cttcctcaac tcttttaaaa aggcattctc aatttgaagg gatcggactt
     6481 gcttttctaa tgccttgata cgctcgcgtt cttcagttgt tgtattttct tttttgtttt
     6541 ctttttttct agtcaaagta agtggtcgcc ctttctcttt tgagagcccc tcaatacctg
     6601 cttctctgaa attacgcatc caattggcaa taagcgcagg gttgttcatt tctaaagtgc
     6661 tggcaacttc tcgataggac atctcacttg tttgatataa ctctagcgca tttaacttaa
     6721 attgaacaga atatttctga ttttgatgct ttttcaatag accttcctct ccgtattcac
     6781 gataggcatt cacccaatca cttacttgag acttgcttga aataccgtat ttgttggcaa
     6841 gatgccgata ttcgcccacg ccactaagat attctttaac gatttttagt tttaattcaa
     6901 aaggatattt tgccatacaa ataacgaccc ccaaaagtta gattttttgg tctaactttt
     6961 gattgatcca tccgtgtacc tttttttgat ctcactcctt gtatttttgc taaatatcct
     7021 aatccacctt ctccttcaag atactcttga acgattgttg acttgaattc aaagctatat
     7081 tttcccataa aaataccatc ccccctgaag ttagattttg gtctaacttt ttagggtttg
     7141 gacatcacca gtcccatttt cattcacgta atgcaagaaa ctgactgaag tcattaaaca
     7201 aaaatctgag ctagaatgaa gttcattctg actcagattt gattttgcac aactcatcag
     7261 tcttcgtaaa atatattttg cctaataagt ttcttttaaa gtatctataa aacacaaatt
     7321 tttttgaata acatattcct tccggaattt atttcgttat tgtgtgattt ctcagagtac
     7381 aatggatgta tggaaaggat tcaacgagaa tttgctaatc agtcctgtag ctttgtctac
     7441 cttcataagt ctgttatcca aagcgctgtg tttactatca actcattagc tcttttatta
     7501 ccgtattcta ttaggcgagg tcgtgaccac acagtgagtt tctgtggaat cccacagtat
     7561 cgaagcattt tctagaattt atatcgaaaa taggttttta ttccatgaaa ttatttgttg
     7621 gtaatgacgt tagttctgaa aaattagacg tttgcttcct aacagacgac gatcagctat
     7681 ctatattgac tgaaaattct gttgctaatg acattgaaag ggctccattg acacgagaac
     7741 tgattcttgt gttcaatgaa aacaatcact tcggtcaaat cgttattgag atggaattaa
     7801 acgtccatgt acagctttca tccttcgatg tttttccatg aagatgcaga gctaaaaaaa
     7861 ctcaatgcac ttgtgaggat tgaaaatcct tttcggatga aacaattcag tcgcatgttt
     7921 gaggaagata cgactgatcg aaacaacgcg ttaagaattg ctgatttatt gcgtattcaa
     7981 cggttcacaa cttctcctat caaagaagag aagtacgtgg ctttacagcg tttaacacgc
     8041 actcgctatc gactcattaa gcaacttcac tcgtacaaaa caacactttc tggaaaatct
     8101 aacgtacaaa tgcaatacgc ttactcgata aatgcgcgat gaatcgacaa gtctctatag
     8161 tgccactgtc attactcttg tgacagaaga cttcacgcta gatgaattgg ctgtgcttcc
     8221 tttggaagct ttctgtgact tactaaaaga aaaaggaaat gtcgctttaa agatccagaa
     8281 aaaaatgcca aatcgattca acgtgcaatt gccgtgagtt atcggttaga tagtcttgca
     8341 taagaatcca tcaatgtcgt tctaaatgtt tttgtatgtg aaattcgtgc gcttgaaaag
     8401 aacatcaaag acttggataa agcaaccgag caatttgttg tcgttattcc agaatatcaa
     8461 tatttgacta gtgttccagg tattggaaag gtacacgccg ctggattaat cgctgaaatc
     8521 ggtcaaattg aacgatttga agatcaaaca aaattggcaa aatacgcagg cttatgttgg
     8581 aaaggcaaac aatctgttaa ctatcggtca caaaatacac ctctgacaaa acaaaggagt
     8641 cgctatttta gatactactt agttgaagct tccaactctg taaaaaaata tcttcctcaa
     8701 tataaagctt tttatcaaag caaatgtaaa gaggtcccaa aaataaacac aaacgagcac
     8761 acgtcttaac cgcaagaaaa tttacgcgtc tggtagatac gctactacgt aaccatcaac
     8821 tctacacgcc accaaggaac gtgatagata aataacattc tgttattgac gcaaaccctt
     8881 gatatacccc gaaaaaattt gattttggcc ggggctagtt aagtacgctt caaaataggt
     8941 tacacaataa aaatattctc aatctccaac ttgtcttatt accattagac ttattgacaa
     9001 agagacaatt accatgctct tttttactat ttagtctact atatattggt ttagacagcc
     9061 ttccaagtct tatttcatca cgcagcctac caagtccttt aaaaataaat tgaagctcgc
     9121 aataatttca gcaaataatc aacagcaata tttttgggtc gtacataaca tgaagtgctt
     9181 gtacctacta gtctcgttaa gtaacagaag attatctttg cttgactaat caataaatta
     9241 agtaaataac aatataatat tatcgagata tttattcata ctatcaatga cgctttaaca
     9301 acgatatgct ccctcatgca attagccagt gttattttca tcatagcctc ttgccttttc
     9361 acatacaaaa atatagttat gatgagtcta ctatacaaat tccagcaata aaagcccaaa
     9421 gcacaaagct aagggttaat aggtgtagca tgaccaaagg agccatcaat catcaatgtt
     9481 tcatatgcaa ggtttgcacc tttgtgaaaa caatcaataa catgttctat cattgatttt
     9541 tcacgtttag ataagacaaa attacttgca aaagctgcaa agtatgcatc acctgcgccc
     9601 attgtatcat gaggtgactc aactttgcga atgaatcctt cataagtctt attctctcga
     9661 gtgtcaacaa aaacagggct cttactacca cgcgtaaaaa gaagatacgg aacctcaaag
     9721 tccttgcatt cactaattaa gcgattaatt tcctcttgat catctgtagc gactgaaaac
     9781 tgtgcaatat aaattgacgg acaaacatct gacaagtatt catgcgttct atatttatct
     9841 aagtcagaaa aatcaaaaga aactttaaca tcatattttt ttaattcttt aatttttgtt
     9901 tctgttcgac tatggcaact agtatgaact aatgaactgt tcttcatcaa atcaataagt
     9961 tctgctgaga gagcaggtgt caatcgggag ccacgcacgc cacctataaa ctccctatct
    10021 ccatctttaa tagttacatg tgcttgctca gtaggctcgt caataacttc cgagaaagag
    10081 atatcaacac cttcagaact gatggcgtgt tgtaataaca cgccatcagg atcagatgat
    10141 agactaccga catagtatgc tttaacacca ctcttttttg cattcacagc aaaattaact
    10201 gcatttcccc ctggaaattt ttgattcaaa ttctcatagt aatcaacaac attatcgcca
    10261 aatccaatca catacgctgt cataattaat cacctacttt tagtattcaa ctactcggta
    10321 ataacgacgt ttatccaaag agtggttccg aatatcttca agatgaacac tgacgcgacc
    10381 tagggctgcc cacataactg gagcacaaaa gtactttcga aattcgtcac taatacctgg
    10441 tagctcataa tccttagtat caaaaacatt aaatttatct gtatattgga ccgcaaattt
    10501 ttcaactcga gcatctaaaa gccgtgtttc cccctcagtt ttcaaaagtg ttacacaaac
    10561 tcctttttcg actaactcga gggtaccatg gaaaaactct ggtgatgaga cagactttgt
    10621 acgcaaccat tgagattcct caagcacaca cattgaataa gagtaggtga ttccccataa
    10681 attcccgcta ccaatccaaa tttgataagg atcttcttta aattcttcgg catacttctt
    10741 ccctttttca tcagcctgtt ctctaactga attcagagcc gttggtaagt ttttcagttc
    10801 atcagcaaaa cgagtgtatt caggaaattc cccattcaaa ttcatgatag acataattaa
    10861 gaaataaagt ggcatttctt gagggcggcc gtctaggtaa ttgattgcat aagttgatag
    10921 agcctctaac ggtgtatctt ctttacttac aataccaacg gttggcacgt ttcgttcttt
    10981 taaatattca gctgccttaa tagtttcttt cgtatctcca gattttgagt ataagattac
    11041 taaagatttt tcattcaact gtttatgtcc cattaagaca agctcagctg cagtcataga
    11101 gtaaacagga atacttgatt cagtcatcaa tacatactca aatggttgca ttacggctac
    11161 cgatccacca gaagcagtaa agaaaatatt actaattcca gaatcataag cttgctttgc
    11221 aattttttct acatcttcac gaataccgta aatccgtttg cctttttttt tcatttcttt
    11281 tgcattatag tttaacatta gttctctcct tacaattatt tagattattc aattaagttg
    11341 tcaaaaatcc gaagaaggct gctatgatac caaagataaa gattccgaat aacacgtaag
    11401 ttgttttaaa gcctttttta agcatccagt aaatcagaaa tacacataat actgacagca
    11461 gatttggtaa aataccatca atgatacttt gaatttttgt tgcttcatca ccttgtccaa
    11521 tagaaccagc tactttgatt gataccatac ttccagacat gcccccaatt accataagac
    11581 ccaaaattga ggcagcatga gtcaacgttt tcatgacgcc agaggattca atgttctcta
    11641 atagctttgt accaaagtta tatccctgca taactccaaa gtaacggaga agaaaattag
    11701 ggacattgaa gattagtaaa aataaaaatg gacccaaaat attgccttct tttgctaatg
    11761 aagcacctac accgataccg ataactaata aggtacccca aaacattgaa tcaccaatac
    11821 cagctaatgg tcccatcaat gcagttttaa tagaagtgat tgtagaagta tcaaagtcat
    11881 caggattttt ggcattttct tcttccatag aagccacaat tcccattatg aatgttgata
    11941 atggcggagc tgtgttaaaa aactctagat gtctttgagc tgctttttgc ttttcttctt
    12001 cattgccact ataaattttt tctaacgctg gcatcatagc aaaagcaaag cccatatgtt
    12061 gttggcgctc aaaattccat gatgcttcta atgcacatga ccgaatacaa atttttgtta
    12121 aatcatcttt agttaaagct ttcccactag aagtcatctt caaccatctc ctcccctctt
    12181 gaacccattg cagctttggt ttccatgttt actataatat aagctagaca tgcgccgaag
    12241 attgcaatcc ccagaatcgg cacatgtaag tagctaaaga gtgcaaaacc aattacgaaa
    12301 tatacaaaga catctttctt aagaagcatt tgcaataaaa tagcaaaacc tagtgctggc
    12361 ataatgcctg cagcaatgtt aatcccatct tggacaaaag ttggaatagc atttaagatt
    12421 cgttccataa caggacttcc taagtaaaat gagaatccga caaataaagc ccgtggtaaa
    12481 ctttttacta agataaaacc taaaacatgc attttcgaca cattttttag ttcgcccttt
    12541 tcagcatatc tatctgcctt tctagcaatc attggtagaa caagcaatgt tagtagatta
    12601 tcaactaata agaatagcgc agcgattggg aatgccaagg tcaacgcaaa ttctgctccc
    12661 ttgccagttg caatagcaaa cgcagtccct aaaatcccac cagataccgt atcaggcgga
    12721 cgtgccgctc cgattgttat tgctccgata aatgctaact ccagtgttgc ccccatcata
    12781 atccccatct taagatcgcc cataattaaa ccaacaaaga cggaagatac gattggtctt
    12841 gataagttat tcgtacctgt catccagtca attacgatga acgcagtcaa gagaccaagc
    12901 aaaattgctt gtactaagag actatccatg aaattacccc cttatttttt tattacaaag
    12961 atttttttaa atctattgcc ttatctgttg gaacttgttg agcgaacact tcaatgccct
    13021 ctgagaccat tcctttaagt tctgctcgtt cctcttctgt tacaaaaatt gtatgcgtca
    13081 aagcgctact gccactagtt tccttaacac taccgatatt tacagattta atttgttcaa
    13141 ccccatttgc caaccgttta gcatcggaaa tggttttggt aactacaaat agtttgtatt
    13201 tatctgtctt cccactatta attgcatcaa tcgaatcttg aattgatttg ataacaacct
    13261 tcacgttatg tggtgcggct aatttcaaag ttgtcttttg aattggatca ttcaaaattt
    13321 catcgttcgc tactaaaatg caatccgcac tgattgcgtt cgtccaagtt aaagcaactt
    13381 gaccgtgtat caaacgatga tcgatcctca ctaatttaat cattctcttc cctcctttat
    13441 ttaaaagtca tcaaccgttt gctcataatc tagctgatta caaaacttaa tcgaaccaga
    13501 agatgcttga attgcatgat caataatttc ttcaatctga tcagatgaaa ctaattggaa
    13561 ttttaaatat aaatccacta aaagacttaa attcattcca gaaatcaaat agactttaga
    13621 aagatctggc aaccgtctta gccactcatt attaatgctt ccaccaaaaa tgtcggttac
    13681 aacaatcgtc tcatttccca tagattcctt tagataatca tcgatgatca tttctacgtc
    13741 attatttcca tcgacatagg cacatcgaaa atcaatatta tccaaatctc ctacgataat
    13801 actcaaagtt tcaatcattc cttttgctag aggaccgtgg ctcatacata atatttgtct
    13861 catacccgaa ctcctttcgc tttgttatta gcaaatactg tgccaattac tgtcaccact
    13921 taaatgaaag ggcgtacaaa aaataaagtg acactatttc catcgctgtg aatagtgtca
    13981 ctttatttta tcaataactt acaatgatgt tgcacacata tgcaatctct tccataggaa
    14041 tcttaatatt ataaagtttc tcaatcttac taagtgcctt ttgaatagaa gcaacctcag
    14101 tttcgtatcg cttaagaaac tgatctattg ctggataact acttactccc tgattacgaa
    14161 ttagtcgctc aatcatacag ctgatatgaa cataaagatt aattttggac aatgatgata
    14221 acgttttccc caccctcatt tgataatctt ttaaactttc ctcaatttga ttgacaactt
    14281 tttcggcatc taaaatagta agactgtcaa tcaagcggtt taatgagcta tttttaacaa
    14341 tattttgatt aattgcctca gcaatctctt ttccatagac tctcaaaaga actttataga
    14401 gtttgccaat ttcaccatca aataattctt caagagaaat atagggaatg ttcgacacac
    14461 gcggatcatc tgttccgata atggcaatga tatcctttgt tttaatgaga aactctaatt
    14521 gctgagtatc tctaagagtt tcattttcgt atggcagaaa ttcaatgccc aagtctttcg
    14581 acaaattatt ttccatcatt tctttaattt ttatggaggt acccaaacca gtattgcagg
    14641 aaacaacgat tgtacgtttt ctaattttga tgggatatag aacttgcttt tcaaactgag
    14701 aatacggcaa tttttttaca accttttgga tatcactcgt actttgaatc agttgtgcgg
    14761 tttcaatagc catttcggtt gtaacattat taataatcat tgttggaact tcgattactt
    14821 gttcaatttc agtcttaatt tgagctaaac ttcccatatc aacgaaaatc actagtccat
    14881 ctttaggctt tcgatactct atatagtgaa gtaatctgtc aattacttca ctaaagctga
    14941 tatctagagg catatcaata gaatctatga caaactgatt aaccaaacga ttggcaacat
    15001 tagcaatact actagctgtc gcatatccat gggctaaaat aattgcgtta atgtctttcg
    15061 cttcatttcc ggtctcacta ataacacaca aactgatata cagtttatcc caaatagtca
    15121 atgttgtatc cagggcgttc tcgaagatca gtaagaatac atttaaatcc gtcaaatagt
    15181 tacactttag gttttcatat aacttttcaa tttcattcgc tgaagcattc tctttcccat
    15241 acccccattg atccctaata gcgagatagg tagctatagc ttggaccgta ttgccagaaa
    15301 ggtgaacaaa aagactggga gagatttttt gaacaatatt ttggatttcc ggtaaataat
    15361 acttaactaa gacttctgaa tttttttggt aaataaaacg atcattaaat tctgccattt
    15421 ggtccttcac aaaacgtcga aactcttcac tatttctatt cacatcatga cactctgaat
    15481 aatatttaga aacacgatga atcaaacctc gtaaatttgt agttacagtt gaagtctcgt
    15541 cacaatcatg aacatagtta ccattactaa agatatagaa ccgttggctc gcaatttggt
    15601 tttttaactc aatattttct gttagcaagt acctgctagg taaatcagaa atatgaattt
    15661 cgatatattt tttattgctt ccactaaata gtttattcgc aatagtttgt tttattagtc
    15721 catccagctc accaatgttt ccatacaaat ctaagcgaat caggaaacta agggcggctt
    15781 cagaaactaa tatttcttta ttgatcagag tagactcttt ctctaataaa tggaagacaa
    15841 tctcttttcg ttcgctaatt tctctatctt ggtaattagg caaagtacaa acaatcggaa
    15901 ttcttcgtcg aaaagtttct agaaaaaaat tttcttcctg ttccgttgtc gcaaaaatat
    15961 atctaacatg tgataaacgc caaggaccac tatctccaac tcttcgaaaa acgcctttat
    16021 ccatatgaat aaaaagtttc tcttgtcctt ctggtggtaa acgatgaatt tcgtcaagaa
    16081 agacgattcc accgtctgct gcttctatta taccaatatg gtcagaatcg gcgccggtaa
    16141 acgatccctt catatggcca aacaactgac ttgttaataa ctctggatta ttaaaatact
    16201 gagcgcagtt taactcgatg aacggagcat tctcatgaat aataccagtt tgtcggcaat
    16261 actcagctag tttttccact aagtaactct tcccaactcc ggacgatcca aaatacatta
    16321 ttggtaatcc acctggatat ttcattgctg atatcccttg ttgaaccgct tcttttaaac
    16381 tttgttttgc accaattaaa tcatcaaaaa aatgatctgc ttttttcttt gaaatttttt
    16441 ctctaaacga atcaattgaa gaaaactcaa aactatctaa tgaatgtgat atataccgtt
    16501 caattgtatg gactggaaaa aaaagaacag gacgaccatt gattttcaca acttttttct
    16561 gttccattag ttcgtttaga tgcttactag ccgtatttct ttttatttga aatttttggg
    16621 caatatattg cgcgttacat tcttctggaa aatattgtgg aaaatcatag ctgttaagta
    16681 gatcatttaa ttcttttaaa attttcgttt tcaaatgaac ccacctcctt tacttaataa
    16741 taaatgattt tcactataaa attcaatatt ctttataaac atctttccac tttaacaata
    16801 cctatggtat ttttcctaaa aaatatttga cactttttat tacctgtggt attttattta
    16861 tgcaaacaaa atattaccat tggtaatcaa aggagagatt tttatgttta attttgatca
    16921 agaaagattt ttaaagactc aagctggtgc cgttgcaaca aatgaacaaa tttccaatat
    16981 tttagaatcc atcgcatctg acttagaaga aatttacttt attgggtctg gaggagttgg
    17041 tattcttttt gattcaacaa tcgaattcgc gagaactcgt agcactttcc cttttcatcg
    17101 tgttatcgct gccgaattta tgattcatcc caataaaaca ttttctaaca aatctttggt
    17161 tgttatccca tctttatctg gtacaacaaa agaaacactt gagttagtcg aatactgtaa
    17221 tgaaatgaac gtcaaaacat tagctttggt aggaagtcct aaaacgcccc tagaaaaagc
    17281 tgcaacatat tctatctata acgacgtttt agatgataca tcttcagaaa attattacct
    17341 tcaaggactg ctgattgctc tcttcttaat gaaacaaaga aatgaaatcg atatcaatca
    17401 atacaatacc tatattgagg atttaaaaaa aatacctgaa gccttagtgc acgctaaaga
    17461 gcaagcggaa ccaatggcaa aatcttttgc aaaaaaacac aaagatactt catatcatat
    17521 tttaaccggt gctggtgata gctttgcgga agcatattac tatggcatgt gtattttaga
    17581 agagatgcaa tggattaaga ctcgtcctgt tcatgcagca gacttcttcc atggaactct
    17641 tgaattaatt gaagcgggtg taagtgtcat cattacagaa ggagaagatg attctcgacc
    17701 tctcacagaa cgcgtgaaga atttcgttcc taagtttaca gatgttcacg taacggttga
    17761 tacaaaggac tatgacttac ctggcatttc ggaagacacg cgaaaaatta tttccccagt
    17821 cgtcttagct actgtctttg aacgaattag tgcttttcta gaagaagaac gtaaccaccc
    17881 tttaactact cgccgttatt ataagcaaat ggaatactaa tcataaaaat atgaaaaagg
    17941 tgaccgcgac tccattgccc atttgattgc ttcattttct ctaatgggag aatagaatcg
    18001 cagtcacctt ttttaggagg atctaaaatg aaaatcgcag ttatctgtag tggtggcgac
    18061 tctcccggaa tgaattcagc tttattagca ataaaaaaaa caattgatag cgaaccagat
    18121 tcggaagtag tcttttttaa gaatggttat gccggaatat ttaaggaatg tattgcctca
    18181 gtttctctaa aagaagttga ggtattcaaa gctggaacac ttattggaac agccagattt
    18241 gagaaaatga gagatctcga aagtcggaaa attattattg aaaacctaaa taagatggag
    18301 atcgacaact taattgttat tggaggtaac ggttccttga aaggagctga actattgaaa
    18361 aatgaatcag aattacaagt atctgtcatc ccagggacta ttgataacga tttccccaat
    18421 accgattata cacttggctt cgatagcgcc ttaaacacta taatccgatc aatcgatgat
    18481 ttaacttcta ctgctaatgc tcatgatcgt attattataa tggaagtcat gggccgaggc
    18541 tgctttgctc ttccagagta cgcttccatt gcaaataata cttattgggt tgagagagac
    18601 gacaaggaat acttacactt aatatgtaaa aaatttgagt cggagttcta ctcgcctata
    18661 ttgcttttac cagaaggtat atctaacaaa caaaaaatca ttgaatttat taccaaaaat
    18721 ttaccaaaag aaaccaaata cttaaatctt gcgtatcttc agcgtggatc atctccaact
    18781 ttttatgacc gatatattgg ctatttgtgg ggtcaattag cggcgaagga attaattgaa
    18841 ggaaaatctg ggatattaag tcaaaaaaat ggtaatataa tgttaatacc ttggtaaaag
    18901 gatggattac tatgattttt gacaaagtaa aagaacaaat tcgaaaagtg tatatcacca
    18961 ctgatcggaa atctgaatta ttgccttcgg aaagagattt ggcccaggaa ttttcggtga
    19021 gtcgtccaac aattcggaaa gctttagctt ctttagaaga cgaaggaatt atttctaaaa
    19081 atgaaggtcg cgggtatcta gtgaaaccag cttcagaaaa atatgttgac cacgaactcg
    19141 actcttttat cggattcttc caagatgcgg ttcagcaagg aaagaaaatc agttctaaga
    19201 ttatccaaca aaccatcata cctgctgatt caacaattag tgaatgtttg gagattgagc
    19261 agaatacttt agtgcttgtc ttggaaagaa ttcgatacat cgatgactat ccaacttgta
    19321 ttgcaaaaag ttgcattccc ctatctgttt tacctgaaat tgttgaacat gattttgcca
    19381 aagaatccct gttctcagta ttagaaagta ggggaattag attagcttat gccaaacgct
    19441 caattgaagt tacaccgcca acagatttag aaaaccttta tcttgattta gaaaaaaatg
    19501 agccagtctt tgtctttaca agtattggat ttgctaaaaa tcagaaaccg tttgaatatg
    19561 aaatatctaa gtaccctgct tataagacaa agtttgaaag tactgtttcc aaataatttt
    19621 taaatatgct taaatgtata aagagcctcg cggataacag ataaagtgtc tttccgcaag
    19681 gctctttttc tacttcctct ttatcttaat ccgctaagta gcctacagtc caaacattat
    19741 catctaatta aggtgtgtgg aggtactcct ctaccctaag tcaaggatag tcgacatagt
    19801 tgttaataag tcttcaaagc aagcctgaac ctttttcatt aggtgtgctt acttcacata
    19861 tgacgctatc aaaaatgata gcacccgttg aatcaaccaa ttttctctaa ttcatatccc
    19921 cattcaaaca actatatttt aaaaactttt ttaatctttg attatgtgta gcaaatttgt
    19981 ctaattttta tttctttgca aaatttttcc aaatttacca gataaaagta attcatattc
    20041 atgattgagt cctttaccaa taaaacctat ttcgcaaaaa ttatttgtaa gcattaagga
    20101 ttttctgctg actttcactg gaaggataac ccaagaattc tgaccaaagt gaaagattaa
    20161 gaaccatctt tgccagtcta tctatgtgct attgtaactg tcaacatcat gcgggcagtt
    20221 attattattt atttgaataa tatttttatt ataaatcagt tttttagatt gtgcataatc
    20281 actaattgct gtgattaatt acagaatatg gctctctggg aaaatgtgcg cgtcatgtat
    20341 tcaattttaa attctaattt gtacaaaatg aatcgtcacg actcgatctg acgcaactcc
    20401 aaatgggggc gccgacctgt accaagtaca gtggcatgtt ctttaaacat ttgaagcaaa
    20461 atctgtccat caaaaacctg tattttcaaa gtgaacaagg tgcgctaaat caggaaatac
    20521 ttacactgat catttcttaa tttacctact taaccaaaat ttaaatcaat atgaaagcaa
    20581 cacttttcaa actaaagagg tctttttacc gcttgatgtt tgcttcgata aaaaagttga
    20641 tgtcggatta gttttcattt taataaaacg tactattcct gaaaaaaatt tatccactac
    20701 taagtattcg gatttttcta atttttaaca aaatttattt cgaaattttc tgatattata
    20761 ttcgcaacat tagtgaaaat tcttagataa attcattctt tcaatctccg aattttagat
    20821 atatgtgaaa tatctgtttt ttgaatgttt gcataagtta aaagatgagc gtgctttagt
    20881 attctttaga atagcttctt atacagggga acgaaaagga gaaatactcg gactgacatg
    20941 tagagacata gatttcgata atcaaactat ctgcttcaac aaaacgttag tagaactatc
    21001 cgagggctct ctatgtatta atcctacaaa aacagacaaa tcaaagggag taatttcaat
    21061 tgataatgga accttacgta ttctaaaaaa tggaaacgtt ctgtaacata aaagaacctt
    21121 tctagaggga ttcggataga taatatagat gatatacctg taatcagcac tatcaaaggg
    21181 aataaaaaaa gggacttcct ttacaaaggc taccctaacc atgtcatgga taaagtagca
    21241 cggaagttcc cagatatgaa gataatcaaa gtccatggct tcagaaagac tcatgctacc
    21301 cttctctttg aagcaggtgc tcaagtaaaa gatgtacaag ataggctagg acattcttct
    21361 tctaaaacaa tcatggacat ttatattaaa gtatcaaaaa atcggcaaaa tgaaacagtc
    21421 gaattatttg attcttacat gaacggatga taagcgaggt caaaaacgga gtcagaggat
    21481 attatgggga taccttttta cctcttaatc ctttaatatc aatcctttaa gcttcgactt
    21541 ttgcaatctt cccttgttcg atatagaccc aaatcgatct atcaataatt atcaaactga
    21601 gtcatataga gacaaaaatt tcaaaaacga cctattaatt ggtcatcgaa tatcatttaa
    21661 aatcaatacg acggagtcaa acacggagtc aaaacaagcc cgctgagagt aaaaaatata
    21721 cagtcccgta ggtaaagtgc cacaggcctg tatatttttt atttgagatt acctgaaatg
    21781 attccattta atttttatct cattttttcc tactagttag tatcaaatac tctcctattt
    21841 tctcaataaa ctattttggg ttacatggta tctgaaacag ctaaaacctt gtcattatag
    21901 caaaaaatca ctttatctcc gtttcatttc agatacattt cacttaagcg gattccaaaa
    21961 aaacaaaaga aaaaggaacc cctagttagc ctatgctttt tattggttcc tctcaaaaca
    22021 cggtcatata acaacggggt ggggtaaatg aatgaaaaag gtggaggaaa cattattcaa
    22081 tagctgtctt aaatacttcg ttataaaatg tgaacaaata aaagacatct atccctattt
    22141 tctacacttc cacttgacta ccatctttga atcgtacaat tttcttaact cttctcgttt
    22201 acctcaattc attctcaagt ggaatcaact catcatctaa cattttacgc catgctcgct
    22261 tcatttcttg tgggtattta aatggcttcg cttctggatg tttggcttta tatccttcgt
    22321 aatccttact gttgataatt gctttcatat taggaattgg atcttcacct ggtagatatt
    22381 gcagattaga agcatgtaat tctttctcag aaacaaacca ctcattggaa aagacatgaa
    22441 tcaaatcatc actcgcttgt gtaaaatagc gacgtttaat gacaaagata tcttcattac
    22501 tatctaaagt ccccacatca aatgcattta aaatttgtcg gtaaacttct ttgactcctt
    22561 ctgttttatt caatttagcc aaagcttttt caatttcttc tcgtgcatct gaactattcg
    22621 cagcataagt tcctagtaat tgatcaatat aagttgaatc aatatcatac aacttagtgg
    22681 tattttcact ataaaattcg atcgttgaaa aatcttctcc ttctttttct ggttcttcct
    22741 ctattaatga acccttcacg gtctcgtaca cgccaatttg ttcttccaaa agttgaattt
    22801 gattgttcaa agttggagat ttcaccatgt catcattgta atcgtcataa gtcactaacg
    22861 attcataagc acgattcaat tcttgaaatg ctttgacgta ctcgatacgg atttcaagtg
    22921 gcgtatgttc atcaatggat tccatggatg aaacctcttt taacttacca tatgcttgtt
    22981 tgaatctttt tttggattcc tgataacttg gataaatcaa acctgattct tcttttgctt
    23041 ttgaatacag ttttgtcgca tcctcgacat tcttctccat tgtcgctggc ttacgaaacg
    23101 tcacaatcaa tccttttgtc ttatcaggat acgtacgatt cgtacgtgaa aaagcttgga
    23161 tcaatcctgc atactctaga ttacgatcca caaatagggt ttgaatcgta ggggcgtcaa
    23221 aacctgttaa tagacgatcc actacaatcg ctaaatcgat ttgtttccca aattgtttga
    23281 attccgctct ttttcgtgcc aaacgattat tgatgtctcc gttataacgc tcaatgtctg
    23341 ctaatgacca ggaagtatca taatactgat tatattcctc aataattttt tgcatttctt
    23401 cttgttgggc acttgaatct cggctatttt cttctaatga ataggtaata gcgattcgtg
    23461 gaaaatcagg atcctccatc gttcgtcctt cacgaatgag ctgattggga aactcttttg
    23521 ttaaccaatc aggctctttc gtcatctctt tgatggcccg ataatacttt ttggccatgt
    23581 caatggaact cgtggttaag attgcagatt tcgttggcct gccatttcga aaatcaaatt
    23641 ttgtataggc attatctgga cggaacattt tatgaatgac tttttggata tgttcatctg
    23701 cttcaaaaac agctgggtcc aaataacttt cttttttgac aggttccatt tgatcaatca
    23761 tacgattgat ctgttctgat gaatactctg catatgtttc tacttctttt aatttccgat
    23821 agattttatt ctctagtgaa gtcggctcca cggtgttttc atgctctact tgaaatccta
    23881 agacagagcc atcttctagc gcattcttaa tggtataggt atgcaagacc tctccatact
    23941 gatcatgggt tgtacgtgcc aattgccctt tggcttgttt ccgattctca ggaaaaatcg
    24001 gtgtacctgt aaagccaaac caagtggatt tcggaaacat ttttttgatt tcttccatat
    24061 tttcagcact taacgctcga tgacactcat ctactacaaa cacgatatgt tggcccatta
    24121 atttttggaa gcgattggtt ccttttttct cttcttgttt ctttgcatat ttcaacgccg
    24181 catctagttt ttgacgggtt gtaatgatta caacatttgc attggcatct gccaacaggg
    24241 tttcacttaa ttctttggca cttcccgttc caacgatcaa gctattcgct ttggcgttcc
    24301 cggaagaaat cccagtatta aattccgaag caaattttgt aaactctgtg gtggtttgat
    24361 tatctaaatc tttgcggtcc actaacatga tcgttcgatc gatccccgat tttcgtgcta
    24421 aaagcttagt agatacaaaa ctagtcaacg tctttcctga accagtcgca tgccacacat
    24481 accctgattg atgtttattt gcggcaataa acaaggcttg aatcgcatgt acttgataag
    24541 gatgtaacac catgagggtt ttattgtctt gatcttcact gacgattgta taatccgcaa
    24601 tcaaacgatg agcatccggg atatttaaca cttgtttaca aaactcatat aagttttcta
    24661 ctttttcatt gtctttcgtt cgccaactaa ataaaaactt agggtgcaag tctttcggta
    24721 aggcatttgc aaaataccga gtggtttgtt cattggaaac cacaaacaac tgtaaggtcg
    24781 aaaagatatt gttccgaaac ataccttctt ctgcatattt tttgatttgg ttaaatgcct
    24841 gataaacacc atcttttgca gttacttgtt tcagctcgat ttggacaatc ggtaatccat
    24901 tgattaataa ggtgacgtca aaacgtcgat cccgtgcttc tatgttactt ccacgttttg
    24961 caatttgatg gaccacttca taagtagaga ttcctccacc aatgtcttga ttggaataaa
    25021 ggataagtga gacagaaccc aattgcgaat cttcccgttc aatcgtgatc cgagccatcc
    25081 cattttcacc ttttaaccat ttcgcagcat caaatggggt tttcgttcgc agcagtaatt
    25141 ccgttttgat cgtttcaaat tctttatcgg tcaaaggaaa atcattcagt tctgcttgat
    25201 tatttgagat aattttttgg cgtaaattga cccaaaggtc ttcttctgat tttagatccg
    25261 gacgatacgt ccactgatta tgcccttctc caagtacacg aatcaactgt tcttcgactt
    25321 ctgcttcctc acggtgacta attttcattc ttcttccctc ctagacaaac atcttttgta
    25381 agaagctttt tttcgtttct tttagtaaat ctaacttacg ttgatgaaga gcgatcgtat
    25441 catctagctg tttgaagaaa gtaccgattt tttgttgttc atcctttaca ggaaccttaa
    25501 cactgatatt ctcaatatca gaactgttat aagaaggcaa tgcccctact tgggagtatc
    25561 ttggcaaatt tatagtttca aacaatattt taccaaaata gatatcccaa gtattattaa
    25621 ataaataagc catggtattg ttatctatta agaaagggct tgtgaccaaa cgttttctat
    25681 taagcattat tgctgcgcca actttagcaa atataatcgc tggcacatct tttattggct
    25741 tccatttttt acgctcaatt tgctcattag atacataatg atttgcattt ctcatttcat
    25801 actcattacc aatgttattc atgtcggaaa ctttaaaaaa tggagtacct tcagaaccac
    25861 cttgctcggc atcaggaaat ccaattcccg attgagtttt acctaattct cccaacttac
    25921 gctcttccca atcttccgta aaaccaggaa accggatttc tggcactttt gctccgtttt
    25981 cagggaacat tttttgtaag aatccttttt tcgtttcctt cagcaaatct aacttacgct
    26041 gatgaagagc gatcgtgtcg tcaagttgtt tgaagaaggt gccgattttt tgttgttctt
    26101 cctcactagg aaacattgct actatatttt taaacttttt tagagacaga acatgttgac
    26161 ctgttccctc tgcttgaata gataattgtc gttggattcc attgttaata cgtcttaaga
    26221 agaagtcgga atcagcagat tttaaagtaa atacaggata aaaatagctt ataatccctc
    26281 tatcaataat gtcattacgg ttaaacacaa acacatcatt atcacttcga ctacggaacg
    26341 tgaaatatcc tctaggtagt acaaagtaac caatattatt ttctgtagtt acttgccgat
    26401 ttgcaaaata gtcttcttgc aaaactatcc ctttttgttg agatgaagtt agcataggaa
    26461 attgattttg tacacttgtc ttttcaacat aatctacaac gaagtccccc aacttcctct
    26521 gttcccaatc atctgtaaat cctgggaaac ggatttctgg ttgtgtatca ttgctcatgt
    26581 ttgaacacct ccagcgcacc ttgtaaccac gcttcatttt caggtgttgt ttgcagggaa
    26641 gagatagctt ctaaaagttt tttttctagc acttgctgtt cttgtcgtat cttcttgatg
    26701 tcatttccga catgaaccat atcaattggt tcttcttctt cgaaagtatc tacatagcgt
    26761 gggatattta agttatagtc attttctttg atttcatcaa aggttgccaa atgagcgtat
    26821 ttctcgacat cttttctttc agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat
    26881 agtttatttt gatttttccc tttaacaaat tcacgactag catcaatgaa taatacatct
    26941 cgagtttgac gattcttctt caacacaatc accgtcgttg gaatcgatgt cccaaagaac
    27001 aagttcgcag gcataccgat cacagcataa atactgccat cttctagtaa tttttgacgg
    27061 atgacccctt ctgcagcgcc acggaataaa actccgtgtg gtaagacgat ggccatggta
    27121 cctgtttcct tcaaatgata gaacccatga agaagaaacg caaaatctgc tttggatttt
    27181 ggcgctaatt tcccataacg attaaaccga gaatcatcta agaaagtcgt atctgctgac
    27241 caattagccg aataaggtgg gttcattact actgcatcaa atgtgtaagg ttcatctgtt
    27301 ggccagtcct tattcaacgt atccccattg cggagattca tttcttcggc gtcgacacca
    27361 tgtaaaatca agttcatttt tgctagattg tatgtcgttg tatttagttc ttgcccatga
    27421 tatttaacgt tatctgggtg tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa
    27481 cccatcgttg gatcaaaaac actaaagaat cgtcgttcct tttgatcgag cgttacgatt
    27541 tgcgccatca tatcagaaac catatgaggt gtgtaaaatt ctccggcttt cttccctgct
    27601 tctgaagcaa attgactgat cagaaactca tacgcatccc cgatcacgtc cccatcgtgt
    27661 tctaaaacat ctacatcatt caatttcttg atgacttcgg taatcgtcac atttcgttgt
    27721 tgctcatctg ttcctagttt ttttgattgg agatccacat catcaaataa cccattaaat
    27781 tgattgtagg tagatgccaa ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt
    27841 gcttgttttg cttgatccgc taagacgttg aaaagatact ctggtgcaat tgcatatccc
    27901 aaaatatcaa cgattgtggc aatcagatct tcttttgatt cttcatctga aagtaattca
    27961 cgatacaaca ttgtttgttt gcttactgtg tcatattcct ctaaggattc atctgctaaa
    28021 agaaccactt gttccaacaa tcgatcggat aaatatttat agaaaatcag tcccaataaa
    28081 taatttttat attctgacgc atccatttta ctacggagat tatctgccgc actaaataat
    28141 ttttgattta attctgcacc caaaaagata cactcctatc tcttatttaa agttgctata
    28201 taccctctta ttttagcata ccccctttta taaaaagcaa aaaaaaaaag aaagaaactt
    28261 attcttgcta ataaactacg cgaaaggagt ctaacgttgt ttgatataac ttagactgat
    28321 cgttaactta ttttttatat ttctacatag ttatctttag aaccatttca gatacatatg
    28381 aaacaattac atatgatttt ttcataaaaa aacagtactg tctagtagta taaaatatac
    28441 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
    28501 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
    28561 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
    28621 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
    28681 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
    28741 aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
    28801 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
    28861 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
    28921 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
    28981 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
    29041 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
    29101 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
    29161 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
    29221 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
    29281 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
    29341 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
    29401 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
    29461 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
    29521 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
    29581 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
    29641 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
    29701 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
    29761 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
    29821 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
    29881 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
    29941 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
    30001 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
    30061 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
    30121 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
    30181 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
    30241 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
    30301 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
    30361 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
    30421 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
    30481 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
    30541 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
    30601 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
    30661 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
    30721 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
    30781 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
    30841 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
    30901 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
    30961 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
    31021 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
    31081 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
    31141 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
    31201 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
    31261 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
    31321 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
    31381 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
    31441 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
    31501 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
    31561 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
    31621 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
    31681 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
    31741 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
    31801 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
    31861 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
    31921 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
    31981 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
    32041 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
    32101 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
    32161 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
    32221 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
    32281 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
    32341 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
    32401 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
    32461 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
    32521 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
    32581 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
    32641 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
    32701 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
    32761 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
    32821 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
    32881 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
    32941 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
    33001 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
    33061 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
    33121 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
    33181 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
    33241 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
    33301 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
    33361 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
    33421 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
    33481 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
    33541 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
    33601 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
    33661 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
    33721 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
    33781 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
    33841 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
    33901 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
    33961 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
    34021 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
    34081 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
    34141 ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact
    34201 gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata
    34261 ccaaacatat ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa
    34321 taacataaaa attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa
    34381 tttataggaa actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga
    34441 cggtatgcat aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca
    34501 catatatacg gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt
    34561 ctaatatagc tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat
    34621 cacatgatcg atttaagttg tactgttctt gtaaaatctg tgttaccaat ggttttgatg
    34681 acttctctaa tccagatgta cctgtcattt ttgaaaaaat aatacctata atagccaaga
    34741 caacaatacc tataatgaca aacttcttgt tcatatttac tttattcaac tttttatctc
    34801 ctatctccaa aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta
    34861 caccaaagat tgctccagct acccctgcta tcatcaaaat cgtacccatt ttctcatctt
    34921 tttctcgata taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg
    34981 ctaacgatac taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc
    35041 catgtacgat ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgtgt
    35101 ctgttccggt tgtctcatct gtttcttgta gtgctccaca aaactggcaa tacttacttt
    35161 catctgttat ttctttgcca cattttctac aatacatgtc ttctcctcct aaaatcattc
    35221 aattgcttat tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg
    35281 gtacacttaa tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg
    35341 tttgccacga acttacacat tgctgtgtaa cattcatttt ttttgctaac tccgtttgag
    35401 aaagtccttt ttcttttctg tatcggctga gattacttac ttttttcatg attctcccct
    35461 cctgttttat tatatacaac aatatgatgt atttcaactc ataaaatata aaaaacttca
    35521 aataccatta caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa
    35581 acagaaacaa tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa
    35641 agaattagct gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt
    35701 aacagagcct aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag
    35761 ccattttcta gacaatgttg tcgtagaatt atgaggaaga gtttttggct ctctatcgtt
    35821 ctcttagcac tgaagatgca gttcgaacaa ttgactatat gaagctatta aaaaggcaag
    35881 aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg aactttaacg
    35941 gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta gtttaataca
    36001 attgggtctt ttctcttcct tactctccgt ccatcatctc ttgttacact tgtaaattga
    36061 tacagaggaa ctatcccagc tcctgataat atcaattctt ttattgtttc atctatccct
    36121 gcccctttcg ctaaatatat taaaaaacta ctacttgctt tcacattgta tagtttctta
    36181 atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc tctgtttttt
    36241 attaaagcat ttttcacgct gttatcccca acattacgta ggcgttcaca aacagtgtta
    36301 ataagttctt tctctgtcat tagatatgtc ccttgccacg ataagccctg taaaacttca
    36361 tgtgcttcat ctaaaatcaa ttgtttttcc ttgctgagtt ctgatcggga attttgagga
    36421 aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata agaaaaatat
    36481 tgatatgtaa aatgaatttt ttccagaagt ggaaggatat cttcattcaa tttttgaatc
    36541 acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg attaaagttt
    36601 cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa aggaacatct
    36661 tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa cttattacat
    36721 ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa ttgttccccc
    36781 gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc agaaagcaag
    36841 acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa aagtttgtct
    36901 ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt tcgaaaatgt
    36961 gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg actgtctttc
    37021 tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt actggaatga
    37081 caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg attatgatcg
    37141 ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat atcaatggtt
    37201 aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag gcagtcttta
    37261 aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa atcggtttca
    37321 ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca tgtcatggta
    37381 aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat tatcactgca
    37441 tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc atcacacccg
    37501 gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc agtaattgct
    37561 tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg aatacttttt
    37621 tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc tacgcttaag
    37681 tatctactat catctttact caaatctact accttcccct tctcatcttt aacagtgtta
    37741 tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt gcctttattc
    37801 tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa ctcatctctt
    37861 gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc tttttttgta
    37921 actggcttct ctaataattt ttcttgatct acataaattg gaatattaat cattgatgtt
    37981 tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca gataataaaa
    38041 acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt tataccgttc
    38101 catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt ttataacagg
    38161 taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta gatattcact
    38221 tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt ctagcatagg
    38281 ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa caatgctatc
    38341 aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa attttctaag
    38401 aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg attaatcaaa
    38461 caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa aggggtgtca
    38521 acatctttgt aaaatttatt tacaacaccc cttttttgag gtatactaat tttaggaggt
    38581 gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa gatactcgga
    38641 acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt tggtcttatg
    38701 attaacaacg gtcaattaat tgacaaaaaa actatctacg aatgggagaa gggaacctat
    38761 ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag tatagaggag
    38821 ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag agatagcatt
    38881 gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg tcaacagttc
    38941 ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt agaaccagag
    39001 atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa aattagtctt
    39061 ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaaa atttgacaat
    39121 aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt agatgaatgg
    39181 ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga aattactgat
    39241 aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac taagaaaaaa
    39301 atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga ggagatgatt
    39361 taatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg atgtataaag
    39421 gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga agaggtttta
    39481 gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata ttatacggtg
    39541 ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg ataactcatc
    39601 agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca aataatcaaa
    39661 tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat tgtcaaaaag
    39721 tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa cagactgcac
    39781 aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg gaaaaaacac
    39841 ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta
    39901 ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc ttcttcagat
    39961 tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg aaagacatag
    40021 acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa tttggtaaag
    40081 ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta gatactgaga
    40141 ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta aaacttggat
    40201 acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact ctatacattc
    40261 caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg cctcgtatta
    40321 cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga gaatctgtca
    40381 aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac atctatgcac
    40441 atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg atggctcatc
    40501 agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca aaaaagctag
    40561 aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac tgccttccat
    40621 ctcataactg atcaatcgat tgagttcgac tgcatattcc attggaagtt ctttggtgaa
    40681 tggttcaaca aagcccatga cgatcatttc agttgcttca gattcggtca ggcctcggct
    40741 catcagatag tagagttgtt cttctgagat ttttgagact ttggcttcgt gttctaaaga
    40801 gacttggctg ttgtggatct cattgaatgg gatcgtatcg gatttcgacc attcgtccat
    40861 gatgatcgtg tcacattcga tatgagagat cgaacccgca ctgtttttgg caaaggttac
    40921 ttgtcctcga tagtttactt ctccgccgtc ttttgaaatt gatttagaga cgatagagct
    40981 agatgtattt ggtgcattgt ggatcatttt tgcacccgta tcttggattt ggtttgctcc
    41041 tgcaaaggcg atggagagca tggtgcctcg tgcacctttt ccatctaagt aaacacttgg
    41101 gtatttcatc gttgtttttg cacctaggtt tccgtcgatc cattcaacgg ttgcgccttc
    41161 gtatgctttt gctcgtttgg ttactaagtt gtacacattg tctgaccagt tttggatcgt
    41221 tgtgtaacgg caatacgcat cttttcttgt aaagatttct acgatggcgg catgcaggct
    41281 gttacttgaa taagtaggtg ccgtacaacc ttcgacatag tggatgcttg ctccttcgtc
    41341 aacaatgatc agcgtacgtt cgaattgccc cgtgttttcc gcattgatcc ggaagtacgt
    41401 ttgtaatgga acatcgactt tgacggcttt tggcacgtaa ataaaggtcc cacctgacca
    41461 tacggctgaa ttcagcgctg ctaatttgtt gtcggttggc ggtacaagtt ttgaaaagta
    41521 ctctttgaat aattcagggt attctttcaa agcagagtca gtgtcagtaa agacgattcc
    41581 taacttttcg aattcatctt tcatattatg gtacacgact tctgattcat attgtgcgga
    41641 agcaccggct agataagctc gttcagcttc tggtatacca atcttttcaa atgtatcttt
    41701 gattttttct ggtacgtctt cccagtcacg agcgggtttg tcacttggtt tttggtaata
    41761 tttgatggca tcgaaatcga tgtctgaaag gtcggggccc catgtttgca ttggcatttt
    41821 gttaaatgtt tctagagatt tcagacggaa ttcaagcatc cattctggtt cgcctttgat
    41881 tcttgacatc tcacggatca cttcttctgt caagccttct cctgtactga aaaccggttc
    41941 gacatcatcg tgaaacccga atttatattc ttctaattct ggtacgccca tgttctcact
    42001 cctcttcctt gtggtaatga tggaaagctg cggtaccatc gttttccatt gcttttccta
    42061 gtgctttcca ggcaagtgtc gcacatttga tccgagcggg aaatttagct acaccaccaa
    42121 gcatagcagc atcgcctaat tggtcgggat ctttgacttc ttttccttgg acaagctgta
    42181 gaaagtctac agttaaatgt tctgcttctt ctattgattt tccgatgacg gcgtctgtca
    42241 tcatcgaggc acttgccgta ctgatggagc agccactgcc gtcaaaggcg atatttttga
    42301 tgattccgtc ttttacatcg acttgcaatt cgatcacatc tccacatgtg gggttgttca
    42361 attcgatctt gcttgtcgag tcagtcaagc ttccacgatg atgcggatgg gaagaatgat
    42421 ccaagatcac ttgacggtat aaattgtcta atttagatag tcccatgttg gaaaaactcc
    42481 tttgttgcca tgattgcttc aactaaacgg tcggcgtctt gcgttgtgtt gtagaaataa
    42541 aagctcgcgc gagcagtcgc tgatacttca aggtacttca atagcggttg cgcacaatga
    42601 tgacctgctc tgacggctac cccttccatg tctaaagctg tcgcgacatc atggggatgt
    42661 aacccatcca aattgaatgc aacgactcct gtccgaaaat tcgggtcttg tggaccataa
    42721 acagtcaatc catcaattgc ttgtaatttt ggcaacacat aagcggctag ttcttcttca
    42781 tatcgatgga tattgtccat accgagtttt tgcaaataat cgattgcatg gcctaatgcg
    42841 atagcgccgg cgatatttgg tgtccctgct tcgaatttcc aaggtagttc tttccatgta
    42901 ctgtcgtata attcgacaaa atcgatcatt tcgccaccaa attcaacagg ttccatttct
    42961 tctaaccatt ttcgtttacc atataaaaca ccaatacctg ttggtccgca cattttgtgt
    43021 ccgctgaaag caaagaaatc acagtctagt gcttggacat caatggccat gtgcggggca
    43081 gattgagcac catctactac gatgaccccg ccgttttcat ggacaagttc agctaattct
    43141 tgaacaggat tgaagacacc taagacattg gatacatgag caatggagac gattttggtt
    43201 ttgtccgtaa tctttttcgc tgcatcctcc atatccagga agccttcttc tgttagttcg
    43261 atatatttca agacagcacc tgttcgtttt gccaactgct gccacgggat aacattagaa
    43321 tgatgttcca tgtaagagag cactatctcg tctcctgctt tgatatagcg gtcgccgaag
    43381 gttctggcta cccaattcaa gcttgtcgtc gtaccacgag taaacagcac ttctgctgtt
    43441 tcttttgcat ggatgaacgc ccgtactttt tctcttgctt gttcataatc atgggtcgcg
    43501 cgttctgcta acgtatggac accgcgatga acattggcgt tatcgtgttc gtaatagtac
    43561 gctaatgcct ggagtacttg ttttggcttt tgggtcgtcg ctgcattatc cagataaact
    43621 aaaggttcat cattgacgtc ttgaaataag ataggaaaat cattgcgtag ctttttagca
    43681 tcgatcatcc attcaacttc ccttcgatta cttcgactaa ttctttccga acttgttcta
    43741 cagggattgc tgttaagaca gaaccgagga atcctcgaat cactaagcgc tctgcttctt
    43801 ctttatgcaa tcctctactc atcagatagt acatttcttc tggatcaact cgtcctacac
    43861 tggcagcatg tccagcagtt acttcgtttt catcgatcaa aagaatagga ttcgcatcac
    43921 cacgtgcttt atcagaaagc atcaagacac gactttcttg ttgggcatct gcaccttttg
    43981 cccctttcaa gatgtggccg ataccattaa aggtcaatgt tcctttttct cggatcactc
    44041 cgtgttgcaa aatatgcccg atcgtatgag gggctttatt ggttactcgt gtatcgattc
    44101 cttggatttg tctgcccgag ctgattgcca cgattttgac ttctgcgtgt gcaccttctc
    44161 ctgctaaatc tgagtcaaag tcagcgatca catgaccgtc attcatgaca ccaattgccc
    44221 agtcgacaga agcatctcgt aaaatgtgcc cacggcgatt catataagaa gtaatatttt
    44281 ctcctaattg atctacggct gaatatttga tttttgaacc agctttagcg atgacttcca
    44341 cgatgatatt acctgatgat tttgctactt gttctttggt tgtctggaaa cgttccagat
    44401 aagaaaattc actatgttca tctgccacga tcaacacatg cttgaagaag tgctcatcgg
    44461 atgcgccatc ttgaatgaat aaggactcga gtggttcatc gatcacgaca tttttaggca
    44521 catacaggaa aataccgctg ttcataaaag ctacatggag tgcagtcaat tggtcttcgt
    44581 tcatgtttac tgcttttttc atataatatt cttggactag ttctggatat tcgatcattg
    44641 cagtgaacaa gtcggtaaaa atgactcctt tttctgctaa ttccacaggc agttgttcaa
    44701 aaatcgtcca tgaaccttgt tggacaagca caggattgtc tttcattgca tcaaatgccg
    44761 gaatcgtgcc tgtttcactg atggtttgtg tttctttcac atcaaataac gaccagcgat
    44821 ggaatttcac ccgatcgata tgcggcaatg gcagttcatc tgctttcgct agtgcgtctt
    44881 gacggaaggt tctcatccaa tcaggttcgc ctttacttaa agaaaaatca tttaccgcgt
    44941 caagataatc gagccagttt ttttctttca ttcttgctcc cccttacgct tcttcttctt
    45001 tatagtcgat tcctaattct ttactgatac cagcgtagcc ttctgcttcc agacgtttag
    45061 ctaggtcagc attccctgtc atgactactc ggccttccat catgatgtga acgacatcag
    45121 gtgtgatata gttcaataat cgttgatagt gggtgatgat caatgcaccg aaattgtctc
    45181 cgcgcatttc atttactcct ttagcaacga ctttcaaagc atcgatatcc agacctgaat
    45241 cgatttcgtc taaaattgca aatgtaggtt ctaacatcaa caattgcagg atctcattcc
    45301 gtttcttttc tccgccagaa aaaccttcgt tcagataacg ttcagccatt tcttctggca
    45361 tatttagtaa agccattttt tcatctaact ttttcaaaaa tgccataaca gaaattttgt
    45421 cgttttcatc acgtttcgca ttgatcgctg cacgcatgaa ttccgcattc gtgatacctg
    45481 gtatctcact tggatattgc atagctaaaa ataagccaag tcgcgcacgt tcatctactt
    45541 ccaagtctaa aacatttttc ccgtcaaaca aaatttcgcc ttctgttact tcatagtttg
    45601 gattgcccat gatagcggca gataaggtag attttcctgt gccgttaggt cccatgatgg
    45661 catggatttc tccggttttc attgttaggt ttacgccttt aaggatttct ttttcctcga
    45721 tcgacacatg tagatttttt atttctaaga cagacatgca ttataccccc aaattctctt
    45781 tcgttctcac tcattttaga ctaattgaga atgaagcgca atgaccttcc ttactaaaat
    45841 caaatatctt ttatttatta ttaaacaatt ttgatttcta aattagaata ggtataaaaa
    45901 agatgagaaa taagccatta cagacttatt tctcatcttt tttgttccat accttatttt
    45961 gcatcagcct ttactggttt aacagagccg ccccatttgt ctgtgatcca ttcttggact
    46021 tctttgcggt gaagcacttc aatcagtttt ttgattttgt cactgtcttc atcaccttta
    46081 cgagtcgcaa taatattcac gtaaggagag ttgtcctttt caagcaatac ggcatcttct
    46141 aatggattca accctgcgcc gtaagcgaag tttgcgttga ttgcaactag gtcaccttca
    46201 tcattttggt acgtagaagc taaaagtgat gggtcgatcg tatggttgaa cttcaagttc
    46261 ttagggtttt cttcaatatc atcgaatgtt gctgtttcca gatctactcc atctttgact
    46321 ttgactaagc cagcatcttg tagaattgtg atgatgcgtc cccagtctga ttcagaattt
    46381 gaagtaatga ttgttgcacc atcttttaat tcggaaacat ctttgacttt tttagagtac
    46441 aagcccattg gttcgatgtg gacagcaccg gcattgacaa agtcatatcc tttttctttg
    46501 atttctttgt tcaaataagg aatatgctgg aagtaattgg catcaatgtc gccttcttcc
    46561 aaagattggt tcggtaatac atagtcatcg aatttgacga tttcaagatc gattccttca
    46621 tctttcaata atggtttcac atgttccaaa atctctgcgt gcggtgtcgg tgaagcgccg
    46681 actttcaatg tcgtgtcgtc tgctttattt gctgaatctt tgctgtcgct gttgttaccg
    46741 catgctgcta atcctacagt cagcaaaagc gctgctgcaa atccaaaaat tttctttttc
    46801 atatcctaca tccccctata tcctattatc ttttgtctgt tttcttagtt aagaaatcac
    46861 cgattccttg aacgataaat acgattagta aaataattaa agtcgcaacg aatgtcactg
    46921 ttagattacc acgctggtag ccttcctgcc aagcaagacc tccaagtcca cctgcgccga
    46981 tcgcgccggc catcgcagta aatccgatca tcgagatcgt tgtaactgtt aatccagaaa
    47041 tcaatgcggg aatactttca ggtatcaaga ccttccagat gatttcccag taacttgctc
    47101 ccattgcatc tgctgcttcc agtacaccag ggctgacttc acgaaaagca atctctacta
    47161 gacgggcata aaatggtgct gcggataaaa caagagctgg aatggctgct tgcgcaccta
    47221 gcatagtgcc tacgattgct ttagtaaatg gaatgatcaa aaccatcagg atcataaatg
    47281 gcgttgaccg gaaaatatta ctgatgatcg atacgattcc ataaatgatt cgagcatacg
    47341 gtttgttttt gcgtccaata gagtaaagga tcaatcccaa aactaatccg atgatcgtca
    47401 ctaagaccat tgagattaat gtcatatata gtgtatcaag gatagcctga ttcatcgttt
    47461 ccgggttgat ttgtttaaaa tctagatatg tttctgaaaa gcttttatcc acgatggatc
    47521 acctcaattc ctactccttg ttctttgatg aacacttctg cttgatctaa ttctttggtt
    47581 tcaccagtca tcaagacagt caatgaacca aaggaacctt ctttcacttg tttgatcttt
    47641 ccatacacaa cgttgacatc gatgttatag cgtcgaatcg cttgggaaat gactggctcg
    47701 ttggcatttc tttcgctaaa ggtcaaagtt gccaaacggc cgcttggatt ttctttgatg
    47761 agttcagcta atagttctgt cgaatcagct tctggttcga tgtcttgttg tacaaagcgt
    47821 ttggttactt cttttttcgg ttgtctgaag acagtcaata cgtctccttc ttctacgact
    47881 ttccccaact ccatcacagc gactttgttg cagattttgc ggatcacatt catttcatgt
    47941 gtgatcaaga cgatggtcaa attcaatttc ttattgatcg ctaacaataa gtctaatact
    48001 tcttctgttg tttgcgggtc caatgcgcta gtagcctcat cacaaagcag gatctcagga
    48061 tcatttgcca atgctcgtgc gataccgacg cgttgtttct gtccgccgga aagttgtgaa
    48121 ggatatgctt gtccgcgtcc ttctaacccg actaaacgca acagttcttc tgcccgttct
    48181 ttacgtttgc tctttggtac gccagctaat tccagcggca gctggatatt ttcaagtaca
    48241 gttcttgacc ataacaggtt gaaatgctgg aagatcatac cgattttctt tcggaatgta
    48301 cgtaattctt tgttttttaa ttttgtaata tcttgatcgt ttacgatcac ttcgccgctc
    48361 gtgggaagct caagtccatt caggagacgg actaatgtac tttttcctgc gccagaatag
    48421 ccgacgattc cataaatatc ccctttttca acgtttagtg aaacgtcatt gacggcttgg
    48481 atgtttccgt ttttcccatt gaatgtcttt ttgacattcg ttagttggat taatgacatg
    48541 ccattcacat ctttctctta tgatttttca aacaaaaaag ccttcgccct cttgccttaa
    48601 gcaaaaggac gaaagcttga acttccgtgt taccaccttg atttaccgtt acttcgcaat
    48661 aacagcctct tccagtactt ggacttagct tctttccgta tactgtcgcg ctgtaacggg
    48721 cactcccgta gtaagtttgc ttacgctcgc ttactctgct ctaagaccat cttccatctc
    48781 attttcgtta cccattttca gcaaacaggg ctctcttcaa acgtccgtga catgtactct
    48841 tcttttcaaa gctttctcgt ttcatatagg attgcttatg attatagcaa ctggaagaat
    48901 cagtgtcaac ttttttcaag tgattttctg attttataat tcgtcaaatt cttttggatc
    48961 aacatctttg aaacttaaga tccacgcatt catttcatct gaatcattca agatattgat
    49021 atcttcaccg attttatcat taacagaaga aactgtaccc gttagcgggc tggcaaattc
    49081 attgaccgct ttttccgctt caacttcgat cagaggttca tcttttttta gcgattgtcc
    49141 tgcttttggc agattggcaa acgtgatgtc tccaagttct tcttgcgctt catttgtcaa
    49201 accaacacag tattcttttc cattaaataa gatccaaagg ttgtcctttt tcttcaaatg
    49261 cttgcctgcc attttatatc agttctcctt cataaatttt ttcattgaat ccaattgcaa
    49321 caagctggtc ctctcgtaag atcagtggtc gcttgatcag cataccatct gtcgataaga
    49381 cttggctcgc ttttgaagcc gtaaactcgc ctacttgatc tttcagtcca agttcccgat
    49441 atttcatccc gcttgtgttg aacaggcgac ggattggtac ttgactcgtt tctagccaag
    49501 tctcaatcgt tgaggccttc ggtacttctt tgatcatatc aacggtttga taactaacgc
    49561 catgctggtc taaccacgct ttggcttttt tgcatgttga acatttcggg taccagtaaa
    49621 aggtgtacaa ttttcttacc tccttgaaga tcttgcaggt gcttcatttt ggtatctcat
    49681 cgataatatc aacccgatac cgatcatgtt ccctaaaatc gaggatcctc cctgactaat
    49741 gaaaggcaat gggatccctg ttaaaggaag taatccgata tttgctccaa tattctcaaa
    49801 gacatggaac agcagcatca tgatcaaacc gcttgcgata taggcgtaaa attcattatt
    49861 cgtatcgaaa cacacccgaa tcatacgata gattaaaata aaatacaaga aaatgacgaa
    49921 tgcaccaccg ataaagccga agttttcacc gataacggta aaaatcatat cggactcacg
    49981 aacaggcaca tagatattgc ttacgttgaa tcctttacca aacaagccac cggtaccaat
    50041 tgccaaaagt ccttgggccg gctgataact cattcctgac gtatcatgga aagggtccag
    50101 ccatgaatca atacgtgcaa actgataaga tttgaatcca actttataaa gcaagtctct
    50161 tccgccttca gtcgtaacca agaagatcgt tcctgctcca acgactactg ccaagattac
    50221 tacaggtgcg atgatgcgcc aagagattcc agacatcagg aaaatcccgc caaagatcgc
    50281 aagaaagaca agcatcgttc caaagtcatc ttgggccatg atcaatgcta aaaccggtat
    50341 tgtcacgatc aagagcttcc caatcagcca aaagtccgat ttcatcgtcc gttcttttac
    50401 ttgcgtatta tgtttagtta cgatcagtgc catcatcaag atgtacgcaa tcttcatcaa
    50461 ctctgctggc tggaaagtaa acgcaccgaa tcgaaaccag tttttagacc ctgttgaagc
    50521 agctaaattc tgatcataga atttgagcaa gagtcccatt accactaacc ccaaaccata
    50581 aaggtaagga gtcagtttcc agatccattt ggaattcaaa tgcatgatga caacgattgc
    50641 tgccgctcca actaaatacc agacaacttg catccctacg cctttcaaga ctgctggatt
    50701 gttcggatca tggctaagtg ctacatataa tgagagcaga ccaatcaaac ataacagaaa
    50761 gactggtaaa atcacgccat aatcaatacg attatcgttc gaagctttta attttttttc
    50821 catgtttcat cccttttctt aaaaaccata ctgctgatta ttatagcggt ccagttagat
    50881 aatggaaagt ctttccatag acatttggga aaaattaact ttccgtttgt gcagttgctt
    50941 gagcaaatgc agtacaaaag cctactgctt atctttagtg tatcatagaa ctttttttga
    51001 tacatgcaat tatctaccag ctgagattct cctcgctgtg agaagtttct gtgctttcat
    51061 tgtatcgaag gatctcttcg gcaatctcta gcagctgatc agaaaccaga ttggttcgtt
    51121 ggatgaaata aaaatatcga tggaaattca acggagtcat tggcacattt tctggaacag
    51181 agcgcttaga caaaatcgag cgaccgattc cttccttcag caacgcaaca attacatcgt
    51241 tacttttcac gatcatcttc ttatcttgga tattcatctc ttcaaaataa cgctgcgtgt
    51301 aatgaaaaac accagagttt gcttctctta caagccacaa tttgcttttt gggtccccag
    51361 cgatgactag ctggtcttcc atcaaagagt atctttgtac gccggaagtc tctaatggct
    51421 tttcgataaa accaaaatcg gattcatgtt tcgctacttg ttctaatgat tcatgagaat
    51481 tcgtgatttc gatctcaaaa tgcactttag gaaacctttt gatcagatgt ttcatcaact
    51541 gaggcaaaag atagacagca aaggtgtggg aagcaacgat ccgacatact tcttcttcat
    51601 tttgttcatt gagcaatttt tgctgagttt cttcccattc ttctaacaaa accagtgtcc
    51661 gttcatataa taagtcggct tgcttagttg gaagcatctc ttttcttccg ttacgttgaa
    51721 aaagatcgat attcaattcc cgctccaact gtttgatttg attcgagact gctggttgag
    51781 ataaaaacag tacttctgct gctctggaaa aatttcttgt ttcatatact gctcgaaaag
    51841 tcgttaataa cttaaacatg gcaccacttc cataactttt tatgataatt actataaaaa
    51901 ctatttgatt taaatataca aaaaaccatc tataatagaa atgtacaaca aaacagttgc
    51961 tgatccaact agtaacttag atgaagacaa tcagctgtct gcttgctcgt aatcatacat
    52021 tcctattcat tttacaatgt attcgttcat cttggtacta aaatctatta aataaaaagg
    52081 agaatattat atatgaagaa ctttttgaaa attattccga tcccgatctg cgggttgatc
    52141 cttgggttag cttcccttgg aaatctgttt aaagactatc accatgttgc tttaggcaat
    52201 ctaactggcg gtatctctat gattcttatg atattcattt ttgttaaatt gtttatctta
    52261 ttcgaacaca cgaaacaaac gttgcatgat ccaatcatcg cctccgtttc accaactttt
    52321 acgatgtcac tcatggttat ttgtacttat tttgtttcat atgaaagtat tgcaccaatt
    52381 gtcaaattca tttggctgtg cgctgttatc ttccaagtga tccttgtctt ttatttcaat
    52441 tatcaccatg ttatcaaagc tgatctttca attgaagcaa tttatccaag ctggtttatc
    52501 atttacgttg gatttggggt catcactgta actgccggga acttcttccc acttattgga
    52561 aaaattttct tttggatttc attgatctgc tatgcgttct tattgccgat catcatcaaa
    52621 cgtgtctttt tcgtaagaaa tatggctcat ccgacacgtc cgctgattac gatcgttgca
    52681 gcacctggtt ctctttgcct aacaggttat cttaaaaact ttgctgaacc taatttttat
    52741 cttgtcgtag cattgttcgc tttatctcaa gtattgtatt tcgttgtgct gtctatgctt
    52801 ccacgtttgt tcaagcttcg tttttatcca agctatgcag cctttacatt cccgcttgtt
    52861 atctctgcaa cagctatatt tactactgtt cattacttca acagtttagg tatctatagt
    52921 agcgtattgg atttcttaaa agttgcggaa tgtctgtttg caagtgtagt tgtcttctac
    52981 gttcttgctc attacgtacg tttcttaatt aaagaacata gcaaacatgg aaaatttgtt
    53041 tctaaaaaaa tcgcttaata atcgattcaa taggttgtga aaaagctgtt ctgcatcaag
    53101 taataagaac ggccaaacaa aaacagctgt tcatgttttt gtactggttg ggcttattac
    53161 cgcagatgca agcttttgaa caccgtttac caagaggt
//
LOCUS       DACIRY010000003        52450 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.003, whole genome shotgun sequence.
ACCESSION   DACIRY010000003 DACIRY010000000
VERSION     DACIRY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.003"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            1..795
                     /locus_tag="IYE22_000111"
                     /pseudo
     CDS             1..795
                     /locus_tag="IYE22_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            935..1861
                     /gene="cysK"
                     /locus_tag="IYE22_000112"
     CDS             935..1861
                     /gene="cysK"
                     /locus_tag="IYE22_000112"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ9505361.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2097..2543
                     /locus_tag="IYE22_000113"
     CDS             2097..2543
                     /locus_tag="IYE22_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ9505362.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2815..4167
                     /locus_tag="IYE22_000114"
     CDS             2815..4167
                     /locus_tag="IYE22_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ9505363.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            4338..4625
                     /locus_tag="IYE22_000115"
     CDS             4338..4625
                     /locus_tag="IYE22_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505364.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4851..5294
                     /locus_tag="IYE22_000116"
     CDS             4851..5294
                     /locus_tag="IYE22_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9505365.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5300..6484)
                     /locus_tag="IYE22_000117"
     CDS             complement(5300..6484)
                     /locus_tag="IYE22_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ9505366.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6752..7450
                     /locus_tag="IYE22_000118"
     CDS             6752..7450
                     /locus_tag="IYE22_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ9505367.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7457..8134
                     /gene="nth"
                     /locus_tag="IYE22_000119"
     CDS             7457..8134
                     /gene="nth"
                     /locus_tag="IYE22_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ9505368.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8260..8793)
                     /locus_tag="IYE22_000120"
     CDS             complement(8260..8793)
                     /locus_tag="IYE22_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505369.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9090..11471)
                     /locus_tag="IYE22_000121"
     CDS             complement(9090..11471)
                     /locus_tag="IYE22_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ9505370.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11461..12108)
                     /gene="recU"
                     /locus_tag="IYE22_000122"
     CDS             complement(11461..12108)
                     /gene="recU"
                     /locus_tag="IYE22_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ9505371.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12172..12711
                     /locus_tag="IYE22_000123"
     CDS             12172..12711
                     /locus_tag="IYE22_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ9505372.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12801..13220
                     /gene="gpsB"
                     /locus_tag="IYE22_000124"
     CDS             12801..13220
                     /gene="gpsB"
                     /locus_tag="IYE22_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ9505373.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13242..13609
                     /gene="rnpB"
                     /locus_tag="IYE22_000125"
     ncRNA           13242..13609
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IYE22_000125"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13818..14984
                     /locus_tag="IYE22_000126"
     CDS             13818..14984
                     /locus_tag="IYE22_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ9505374.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15030..16526
                     /locus_tag="IYE22_000127"
     CDS             15030..16526
                     /locus_tag="IYE22_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ9505375.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16607..17290
                     /locus_tag="IYE22_000128"
     CDS             16607..17290
                     /locus_tag="IYE22_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9505376.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17935..19125
                     /locus_tag="IYE22_000129"
     CDS             17935..19125
                     /locus_tag="IYE22_000129"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ9505377.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19227..20180
                     /locus_tag="IYE22_000130"
     CDS             19227..20180
                     /locus_tag="IYE22_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ9505378.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20249..21598
                     /locus_tag="IYE22_000131"
     CDS             20249..21598
                     /locus_tag="IYE22_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9505379.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21781..22104)
                     /locus_tag="IYE22_000132"
     CDS             complement(21781..22104)
                     /locus_tag="IYE22_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505380.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22233..23396
                     /locus_tag="IYE22_000133"
     CDS             22233..23396
                     /locus_tag="IYE22_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505381.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23466..24815
                     /locus_tag="IYE22_000134"
     CDS             23466..24815
                     /locus_tag="IYE22_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ9505382.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24860..25291)
                     /locus_tag="IYE22_000135"
     CDS             complement(24860..25291)
                     /locus_tag="IYE22_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9505383.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25679..28321
                     /gene="alaS"
                     /locus_tag="IYE22_000136"
     CDS             25679..28321
                     /gene="alaS"
                     /locus_tag="IYE22_000136"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ9505384.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28521..29117
                     /locus_tag="IYE22_000137"
     CDS             28521..29117
                     /locus_tag="IYE22_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9505385.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29239..30387
                     /locus_tag="IYE22_000138"
     CDS             29239..30387
                     /locus_tag="IYE22_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ9505386.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30425..30838)
                     /locus_tag="IYE22_000139"
     CDS             complement(30425..30838)
                     /locus_tag="IYE22_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ9505387.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30917..31348
                     /locus_tag="IYE22_000140"
     CDS             30917..31348
                     /locus_tag="IYE22_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ9505388.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31447..31680)
                     /locus_tag="IYE22_000141"
     CDS             complement(31447..31680)
                     /locus_tag="IYE22_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ9505389.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31863..33533
                     /locus_tag="IYE22_000142"
     CDS             31863..33533
                     /locus_tag="IYE22_000142"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ9505390.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33680..34066
                     /locus_tag="IYE22_000143"
     CDS             33680..34066
                     /locus_tag="IYE22_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505391.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34053..34751
                     /locus_tag="IYE22_000144"
     CDS             34053..34751
                     /locus_tag="IYE22_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ9505392.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34756..35220
                     /locus_tag="IYE22_000145"
     CDS             34756..35220
                     /locus_tag="IYE22_000145"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ9505393.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35217..36128
                     /locus_tag="IYE22_000146"
     CDS             35217..36128
                     /locus_tag="IYE22_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ9505394.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36363..36902
                     /gene="pyrR"
                     /locus_tag="IYE22_000147"
     CDS             36363..36902
                     /gene="pyrR"
                     /locus_tag="IYE22_000147"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ9505395.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36916..38199
                     /locus_tag="IYE22_000148"
     CDS             36916..38199
                     /locus_tag="IYE22_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ9505396.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38212..39138
                     /locus_tag="IYE22_000149"
     CDS             38212..39138
                     /locus_tag="IYE22_000149"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ9505397.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39157..40437
                     /locus_tag="IYE22_000150"
     CDS             39157..40437
                     /locus_tag="IYE22_000150"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ9505398.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40442..41518
                     /gene="carA"
                     /locus_tag="IYE22_000151"
     CDS             40442..41518
                     /gene="carA"
                     /locus_tag="IYE22_000151"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ9505399.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41523..44708
                     /gene="carB"
                     /locus_tag="IYE22_000152"
     CDS             41523..44708
                     /gene="carB"
                     /locus_tag="IYE22_000152"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ9505400.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44762..45538
                     /locus_tag="IYE22_000153"
     CDS             44762..45538
                     /locus_tag="IYE22_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ9505401.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45535..46467
                     /locus_tag="IYE22_000154"
     CDS             45535..46467
                     /locus_tag="IYE22_000154"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ9505402.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46464..47180
                     /gene="pyrF"
                     /locus_tag="IYE22_000155"
     CDS             46464..47180
                     /gene="pyrF"
                     /locus_tag="IYE22_000155"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ9505403.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47187..47816
                     /locus_tag="IYE22_000156"
     CDS             47187..47816
                     /locus_tag="IYE22_000156"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ9505404.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47804..48118
                     /locus_tag="IYE22_000157"
     CDS             47804..48118
                     /locus_tag="IYE22_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505405.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48156..48797
                     /locus_tag="IYE22_000158"
     CDS             48156..48797
                     /locus_tag="IYE22_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9505406.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48828..49532
                     /locus_tag="IYE22_000159"
     CDS             48828..49532
                     /locus_tag="IYE22_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ9505407.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49529..50431
                     /locus_tag="IYE22_000160"
     CDS             49529..50431
                     /locus_tag="IYE22_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9505408.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50589..52295)
                     /locus_tag="IYE22_000161"
     CDS             complement(50589..52295)
                     /locus_tag="IYE22_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ9505409.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        17146 a         9140 c        11269 g        14895 t
ORIGIN      
        1 tggaacgagt cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc
       61 agatgaacaa ggatctcaaa cagttgctga tcctcaagcg tttatcgatc aaatcggctg
      121 gtctgcgtca gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc
      181 gttgttggaa agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt
      241 cggtgtgaaa ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaac
      301 ggacaatggg taacgatgaa tgaaccattc cgaaaatatt cttcttattg ggaatccttc
      361 caagaccatg cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct
      421 ggcgtgtgga aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt
      481 tatgctaccg atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta
      541 acccgttttg atacgccatc aacaggttcg gctaccacaa gtacaacaac aacgactact
      601 acgacgactt caacgtcatc atcttcaaca acagatgtaa atacaaataa tggtcagaca
      661 tatacagtcg tgtcaggtga tacgctatgg gatattgcac aaaaatttgg catgtctgtt
      721 gatgatctaa tggcgaaaaa tggcatgtct atgtccagtt attctttatt agttggtcaa
      781 gaactaaacg tttgatttag ataaaaaggc aaagaaacta ccatatatgg tatatctttg
      841 tctttttttt gctagtcaaa gacgaatcac tcgccataaa tgaacagatt gtattatgat
      901 aagtaagaag agcaaagaca aggaggaatt gaagatgaca gctatctata actcagtcac
      961 cgaattgatt ggcaaaacgc caatcgtcaa attaaataaa atcgtaccag aagattcagc
     1021 agatgttttt gtaaagcttg agttttttaa tccaggagga agtgtaaaag atcgtattgc
     1081 tttaagtatg atagaaaaag cagaacacga tggtctactg aaaccaggtg atactatcat
     1141 tgaacctact tctggaaata caggaatcgg attgtcgatg gtaggagttg caaaaggata
     1201 taaagtaatc atcgtgatgc ctgagacgat gagtatcgaa agacgtttat tgatgaaagg
     1261 gtacggcgca gagttgattt tgactcctgg tgcggatggt atttctggat caatcagaga
     1321 agcagaacgt ttggcaaaag aaaacggcta ctttttacca ttacaatttg aaaatgaagc
     1381 aaatcctctt gtgcatgaga agacgactgg tcctgagata catcaagcat ttggagtaaa
     1441 tggcttggac gcgtttgttg caggtatcgg tacaggagga acgatcactg gcgctggccg
     1501 agaattaaaa cgtgtgtatc cgaaaatcga attaatcggg gtagaaccag cagaatctgc
     1561 tattttagaa gggaaagaag cgggacctca caaaatccaa ggaatcggta caggttttgt
     1621 tcctaaaaca ctggatactt ctgtgtatga taaagtcctt tctattagtg gagacgaagc
     1681 gatggaaaca gctagagaag ttgggcgaaa agaagggatt ttagtaggca tctcatctgg
     1741 cgctgcaatt gctgccgcgc taaaagtcgc aaaagaatta ggaaaaggca agaaggtatt
     1801 agctgtggtt cccgacaatg gtgaacgtta tttatctaca gccctttacc aagagatata
     1861 aggcacttgg tttttctcca gataggttta tgtaaaaaac ttttaaagat gaaaagaaca
     1921 cacaaaaatt tatgatgaga aaaattattt gaatagagac gtaaaatagt gaacgcgact
     1981 gttttacgtc ttttttcatt aaaatacaat aaaattgaat tttcttttca tgttaagtgg
     2041 attttaaaat gtaaaaatag tatgataaga cgagaataac aacgtggggt gtttacatgg
     2101 aaaataagtt agtcgaagag gttatcgaaa agttaaaaaa agagaacatt cgaatcacac
     2161 ctcaacggta cgcggtttta gaatatttga ttgaacatca ttcccatcca acggctgatg
     2221 agatttataa agcacttgaa catcggtttc caaatatgag tgttgcgact gtttacaata
     2281 accttcgctt atttacttcg attggatttg ttcaagaaat gaagtacggc gatgcttcta
     2341 gccgatttga tttcagttca aagcgtcatt atcatgtaat ttgtgaaaac tgcggtaaga
     2401 ttgttgattt tcattaccca gggcttgaag atatagaaat ggctgcgagc agactgactg
     2461 attttgagat taatgaacat cggttggaat tgtatggact ctgtccggat tgtaaagagt
     2521 cagcaaaaaa ggcgcaaatg taaaaacagt ttctataagt gttataccta aaaaaagcta
     2581 gctgtgtaaa aaaacgctag cttttttatt tttttgtctt ttattcacaa tcttagatat
     2641 caaagtgaat attatcacaa taagaatctt gtagatattg ggttcttatg cttgaaaagg
     2701 aatgaaacta caaacaaacc tttgtgaatt cacaagcatt aaactgttgt atccgtttta
     2761 tttctgttat agtagttttg tcgaaggaca aaatgttaat cggagggacc ataaatgaaa
     2821 gtagtagtaa ttggctgtac tcatgcaggt actgcagctg taaaaagtat tttaaaaaat
     2881 catccagaag cagaagtgac agtatatgaa agaaatgaca atatttcttt cttatcttgc
     2941 ggtatcgcac tttatgtcgg cggagtagta aaggacccag caggcttgtt ctactcaaat
     3001 cctgaagaat tatcttcatt aggagcaaca gttaaaatgg aacacgatgt aacagacgtc
     3061 gatacagaaa ataaaacagt aacggcaaaa gatctgaaaa caggagaaac agaaacggtt
     3121 tcttatgata aattagtgat gacaactggt tcatggccta ttatcccacc gattagtgga
     3181 atcgatgcga aaaatgtact cctatgcaaa aactacaatc aagcaaatga aattattgca
     3241 caagcgaaag aagcaaaacg tgtagtaatt gttggtggtg gatatatcgg tattgaatta
     3301 gtagaagctt ttgtcgaatc tggaaaacaa gtgacattga ttgatggatt agatcgcatc
     3361 ttgaataaat atctagataa accattcaca gatatcttag aaaaagaatt gacagaccgc
     3421 ggtgttactc ttgcgctagg cgaaaatgtt actgaattta tcacagatga agaaggaaaa
     3481 gtgaaacaag tagctacacc aacagataca tttgatacag atatggtcat tttatgtgtg
     3541 ggcttccgtc caaatacaaa actgatcgaa ggaaaagtag atacactgcc aaacggggca
     3601 atcaaagtga atgaatacat gcagacaagt gatccagaca tttttgcagc tggagactct
     3661 gcagtagtca actacaatcc aagtggcacg caaaactata ttcctttggc aacaaatgcc
     3721 gttcgtcaag gtttacttgt gggaaataac ttgacagatc ataaaatggc gtatcgtggt
     3781 actcaaggaa cttcaggact ttacctattt ggctggacga tcggttcgac aggtgtgacc
     3841 aaagaaagcg caactttgaa cgatctagaa gtccaagcga ctgtatttga agataattat
     3901 cgtccagagt tcatgccaac tactgaaaaa gtaatgatgg agctagtcta tgaaaaagga
     3961 acgaatcgta tcgtaggtgc tcagttcatg tccaaatacg acattacaca atcagcaaat
     4021 accatgtctt tagctgttca aaataaaatg actgtagaag atctcgcact ttcagatttc
     4081 ttcttccaac cacattttga ccgcccatgg aattacttga acctacttgc acaagaagcg
     4141 ctaggcgaaa tcgaaactgc acaataaaga aaaagggttg tgacacaatt ttgtcacgac
     4201 cctctgtctt tttttgtgaa aagtacaatt agttgtcttt cttcgaattt tttgtcacaa
     4261 taagcagcaa agataaagct gttacataaa agaacaagca gctttatttg attttgaaaa
     4321 gaaaggtgag aaagaagatg aataaagaat attttgtcca acaagatgca tttttagaag
     4381 attataaaag tgcttcgaag aaattaacga tcaatattga tgagttggca gatcaagtac
     4441 tctctcagtt gaatgaaacg caagaggaat attacacgat gccagcaaat gacgccaaag
     4501 ataatgtttc tcatcgtttt cctttcagta gacgcatcta ctcagatgaa aatgacggca
     4561 cattgaatac agagtacaca tatgaaggtc agccatatgt gctagaaact gatgatgatc
     4621 tttaaagaca gtttttgaaa aggccgctca tccttaagtg atgacaaaaa atcatcacaa
     4681 ttaccgatga ctgtctcttt actatctttt aatcagaaaa aggggttatg acaagctttg
     4741 tcaacccctt tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt
     4801 tgaataggaa acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa
     4861 gacaactgct aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac
     4921 cagaaatgga aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata
     4981 tcgggaaaat ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag
     5041 gagccatcag taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa
     5101 gcgaagagaa taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga
     5161 atagacacga gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata
     5221 tgtcaaaaga agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac
     5281 gaagtagcaa atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca
     5341 aaaatccgaa taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg
     5401 ggccgatgac gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct
     5461 ggttatctga gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac
     5521 cgaaaccata acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata
     5581 atgtgatgaa tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt
     5641 ctgaaaattt tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc
     5701 caatcaaaga gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa
     5761 attgagaaaa gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca
     5821 ataaaggaat aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat
     5881 gggagaaact taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt
     5941 agccataaac ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc
     6001 ccaactcagc taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag
     6061 cacccatcca tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag
     6121 catataaggt ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca
     6181 tccatatcga atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga
     6241 taataaggat cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata
     6301 agaaggcagc tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta
     6361 tggagtgaga ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta
     6421 atccagtgaa taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt
     6481 acatgtaaaa actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa
     6541 ttttgtttcc ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga
     6601 aaagtgaaga tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa
     6661 tctgaccgat cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt
     6721 acgtatactg tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc
     6781 tggacaaaca gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc
     6841 tgaagaattt ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc
     6901 tgatttgcag atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact
     6961 ggatcgtctc gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa
     7021 agcagatcga tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca
     7081 agaaaaaaaa tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt
     7141 tgaacaagaa tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct
     7201 agaagaagac cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa
     7261 tcaggcttat agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct
     7321 acgtacaaaa caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag
     7381 agaagtagaa cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt
     7441 ggaggagtag cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta
     7501 tggaatgttt cccgaagcac atggagaact caagcacaat aatccatttg agttattgat
     7561 tgcagtgatc ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt
     7621 gtttgcctct tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct
     7681 taaaataaaa acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca
     7741 gcagctgatt gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct
     7801 tcctggtgta ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc
     7861 aatcgcagta gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga
     7921 tgctacggtc atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt
     7981 aaagacacat catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa
     8041 gtgcgaggta tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa
     8101 agaaaaggcg ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa
     8161 tgtatgaatt atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa
     8221 aagtaaatgt acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa
     8281 ataaaagttg ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat
     8341 acctataaaa taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa
     8401 aggtgtaata aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact
     8461 aatggatttg ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca
     8521 taaaaaagtg agaatcattg catgagagct tctttccata aattttcctt taagagtagt
     8581 gcttaaaagt acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt
     8641 taataataaa caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga
     8701 taaaaaaaga tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat
     8761 gaagccttct ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac
     8821 aatgggatta ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat
     8881 tataaacaag taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt
     8941 tttaagctaa atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta
     9001 atacagcttg gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa
     9061 ccacgcttgt agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg
     9121 gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca
     9181 ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt
     9241 gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat
     9301 tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga
     9361 gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct
     9421 gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc
     9481 cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag
     9541 gctacgtgtg acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg
     9601 gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa
     9661 ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga
     9721 tataacccag cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg
     9781 tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt
     9841 tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct
     9901 ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc
     9961 ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg
    10021 atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg
    10081 actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat
    10141 tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta
    10201 atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt
    10261 ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg
    10321 tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata
    10381 agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg
    10441 atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct
    10501 gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta
    10561 tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt
    10621 acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac
    10681 aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa
    10741 ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc
    10801 ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact
    10861 ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc
    10921 atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag
    10981 gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag
    11041 ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg
    11101 cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt
    11161 ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg
    11221 gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac
    11281 cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg
    11341 atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa
    11401 ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt
    11461 tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc
    11521 gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat
    11581 caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa
    11641 atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca
    11701 gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt
    11761 aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg
    11821 atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc
    11881 agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat
    11941 aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg
    12001 catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg
    12061 agaggcattc ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac
    12121 tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag
    12181 tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat
    12241 gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa
    12301 gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa
    12361 atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt
    12421 tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa
    12481 ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag
    12541 aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa
    12601 ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc
    12661 tacgaaattc ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg
    12721 tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa
    12781 tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac
    12841 aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca
    12901 atatcatcaa agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg
    12961 atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg
    13021 tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct
    13081 tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca
    13141 aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg
    13201 aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt
    13261 ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc
    13321 gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa
    13381 ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac
    13441 ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct
    13501 ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc
    13561 tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca
    13621 tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa
    13681 gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg
    13741 gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat
    13801 aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa
    13861 gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg
    13921 agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt
    13981 tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg
    14041 aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt
    14101 ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga
    14161 tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac
    14221 cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga
    14281 tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag
    14341 gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa
    14401 aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct
    14461 taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt
    14521 ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg
    14581 atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag
    14641 ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag
    14701 acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc
    14761 taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat
    14821 taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac
    14881 aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt
    14941 attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc
    15001 attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa
    15061 aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta
    15121 tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt
    15181 tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg
    15241 aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg
    15301 aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg
    15361 gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc
    15421 aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt
    15481 atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat
    15541 ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg
    15601 aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag
    15661 tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc
    15721 acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa
    15781 cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac
    15841 aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc
    15901 atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa
    15961 agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca
    16021 aattttatcg tttattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt
    16081 tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag
    16141 gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa
    16201 tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct
    16261 ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt
    16321 tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa
    16381 actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca
    16441 tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt
    16501 attttaatgt atatcaggta aaataaaata caatttacaa agatagctga aattcatcta
    16561 atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca
    16621 agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga
    16681 tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga
    16741 ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa
    16801 aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga
    16861 ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc
    16921 tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc
    16981 attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat
    17041 ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac
    17101 gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga
    17161 ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt
    17221 ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa
    17281 caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc
    17341 cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc
    17401 gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt
    17461 gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt
    17521 tgtcacagtc cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa
    17581 aattaaaaat tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa
    17641 taaataaatt aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt
    17701 gtgttgtatt tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc
    17761 atactatata agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa
    17821 ttctcataat cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga
    17881 cacgatatat gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct
    17941 tccaaagaac taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta
    18001 tacaatacga tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa
    18061 aagatgatca atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa
    18121 aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc
    18181 aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca
    18241 gaagcagcaa tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta
    18301 atgacaaaag aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt
    18361 tgccgcttat ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag
    18421 aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat
    18481 ggtgttttct ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa
    18541 aaatatggac tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt
    18601 gcaggaaccg tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta
    18661 tcgaaagcta taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg
    18721 ttgaaagcta gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca
    18781 gctgcattgg cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg
    18841 gaaaatgcta attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct
    18901 gaaacaccta ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag
    18961 aagctgatcg aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg
    19021 acaggcagga tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca
    19081 gtagcagttt atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt
    19141 tcgtctattt aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt
    19201 agtaaaaaag atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac
    19261 aaattggttc agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg
    19321 cgacagatat acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg
    19381 taatggatta cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc
    19441 atttggctgc tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga
    19501 acgtgactgg gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta
    19561 cgccaagttc aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata
    19621 ctcttcagcg tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg
    19681 attattatta tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt
    19741 cttatgcaac cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag
    19801 caatcaaaga aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt
    19861 atatgccaga tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg
    19921 ttcatcgaaa tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat
    19981 ctattcaaaa aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt
    20041 caatagcaga atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct
    20101 tttcgccgaa atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa
    20161 aattggtaga gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga
    20221 ttggctatgg tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga
    20281 agtaaaaata gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt
    20341 tatgggcaca gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac
    20401 aggatccgcc agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc
    20461 aggactaaca ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata
    20521 tatcgaacgg atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat
    20581 ttattttcca gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct
    20641 atttggtctt tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct
    20701 gttgatcaat tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg
    20761 caagcttatt cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga
    20821 cttccaactg tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc
    20881 gggcttgcta gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg
    20941 agaattgaaa aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat
    21001 catgatcgtt tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt
    21061 cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg
    21121 aagactggtc acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct
    21181 tactggaatg cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct
    21241 ctttgctaaa cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt
    21301 catcgctatt ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt
    21361 tgtcatttgg attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga
    21421 acctgatttg tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat
    21481 cattggaggg acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat
    21541 ggggatagta ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct
    21601 aaaacattcg aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat
    21661 gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt
    21721 ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt
    21781 ttactttgat ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac
    21841 caagcaacca gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt
    21901 agacaaagca tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag
    21961 attgaagact agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt
    22021 agttcttttt cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc
    22081 aaaaacaaag tacatattca gcatctcgac accccctttg tgtttatctt actttacttt
    22141 gaaagaaatg agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat
    22201 aagagagata caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt
    22261 ttgcgctgat tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg
    22321 aatatcgaaa gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt
    22381 tttatttatt gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg
    22441 cccaaatgac agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag
    22501 acaacacggt attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg
    22561 tatttttgga accagctttt aatgttttat tactcatacc atttggcatt tatttaagat
    22621 attatttccg gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt
    22681 ttgaattgac gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg
    22741 atgtgaatga tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt
    22801 tatttacatt tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa
    22861 agactgtctc attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc
    22921 ttattaattt ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt
    22981 ctcatatctg gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa
    23041 ccaatggatg gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg
    23101 aacgaatttc tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg
    23161 gtttaagctg gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt
    23221 tgcagatgag tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt
    23281 taaatatctt gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc
    23341 acacatatac tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga
    23401 gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg
    23461 cacgaatgaa taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg
    23521 caaagggctt caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag
    23581 gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc
    23641 ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa
    23701 gtagggaact agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac
    23761 cggagatccg tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc
    23821 tgcatcacca acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga
    23881 atgaacaagc gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga
    23941 cattggatat gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc
    24001 tgcagttttt agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat
    24061 acatggaaaa tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg
    24121 aaaattggct gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga
    24181 caattggtca tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa
    24241 ttgccgatta cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc
    24301 caagagagag aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag
    24361 cgactgattt agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag
    24421 aaattccaga agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct
    24481 taaaaggaac ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag
    24541 aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat
    24601 acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga
    24661 ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt
    24721 gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta
    24781 ctgctcgtaa aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc
    24841 gtgtcacaat ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat
    24901 cttcaataaa aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg
    24961 tcatttcagc tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag
    25021 cttcaagcaa ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt
    25081 ccggcttgaa gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt
    25141 gatcaagaaa tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg
    25201 caggaatccc ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta
    25261 tccattcatc cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag
    25321 atgtattgat tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat
    25381 gaaaataaaa attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga
    25441 aaaggatatg atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat
    25501 agtcactaat caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg
    25561 ttatcgagtt taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt
    25621 tcgtccttgg ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat
    25681 gaaacaatta agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg
    25741 acatacgatt gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat
    25801 caactctggt gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc
    25861 aagaatcaca aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac
    25921 agcacggcac catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa
    25981 aaatgaagcc atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga
    26041 tccagaaaaa ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca
    26101 tgaagaagtt ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat
    26161 cggtgcaggc ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa
    26221 cgatttagca gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga
    26281 aatctggaac ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact
    26341 tcctcataaa aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga
    26401 tgcgtcaacg aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct
    26461 aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc
    26521 agatcatatt cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg
    26581 gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg
    26641 tattgaacaa gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta
    26701 ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga
    26761 acgtttcaat gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt
    26821 aaaaaacgaa aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata
    26881 tggcttcccg gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca
    26941 tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac
    27001 tgaaaaatca atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt
    27061 cattggatac acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc
    27121 tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta
    27181 tgcagaaatg ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat
    27241 cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga
    27301 agtaaaagca ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg
    27361 caatcggatc atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt
    27421 acttggcgag catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga
    27481 ctttacccac tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa
    27541 cgaaaaaatc tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa
    27601 aaacatgggt gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa
    27661 tattggcggc tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat
    27721 cggaatcttc aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc
    27781 tgtaacaagt aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt
    27841 agcaacaatt gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca
    27901 acagcaagta agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca
    27961 acaagctggt gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc
    28021 acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa
    28081 aaatatttca gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc
    28141 ggcaatgacg aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat
    28201 cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa
    28261 aaatcctgct ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata
    28321 gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg
    28381 ggcgaagaca aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa
    28441 atggtcggat tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat
    28501 aaataaaaag gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg
    28561 cgatcttgtt agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat
    28621 taggattcat tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag
    28681 gcccaaaaga tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct
    28741 atgccatgcc aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag
    28801 ttcccagagt acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa
    28861 ataaatcaat aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag
    28921 tcttagtgtt aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg
    28981 tagactctgc tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag
    29041 aagcaatagg tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac
    29101 cgttgaaacc agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa
    29161 aggaagagag gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa
    29221 ctagaagggg taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa
    29281 cagcggacaa aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg
    29341 tttgtctcta agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca
    29401 gtgaacagtc tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct
    29461 gcaaagccag cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg
    29521 ttatttgttt cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa
    29581 aaaatcatcc atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt
    29641 tccatctttt tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat
    29701 tctgaaacat tgagagctac agcaacagat agttataatg atgtctttac tacactggct
    29761 gtattgcttt cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt
    29821 tttgctttag cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac
    29881 gaactattag gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat
    29941 gcttgcgatg atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa
    30001 cgctttgcat cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag
    30061 attattgatc tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac
    30121 ttggatccaa tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa
    30181 attcttcttg aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga
    30241 aaaacgctgc aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta
    30301 gaaagaatct tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg
    30361 tttgatcaca attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt
    30421 taatttaaag agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat
    30481 ctgctccttt atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata
    30541 gaagttcttg gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt
    30601 cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt
    30661 cgtttttctt tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg
    30721 tccctataga aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc
    30781 cacctccact aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa
    30841 aaagagattc tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc
    30901 ggtgagaggt tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat
    30961 catctattgg tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa
    31021 cacaggtcct tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct
    31081 aggtttgcgg cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt
    31141 ttggaaagac cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg
    31201 ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga
    31261 tcaagtagtc ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta
    31321 tccttatacg aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt
    31381 ttacaggaaa agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt
    31441 gtttatctat tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc
    31501 agcttgtgtt cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa
    31561 agttttgaat ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc
    31621 ataactgata aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa
    31681 agcactcacg acctttctaa agtctgatca tagccttcat tataaagagt atttataagt
    31741 acgtcaaata aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata
    31801 attggcatat cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc
    31861 aaatgaagaa tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg
    31921 ctagtaaatt agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa
    31981 tcaatttttc tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta
    32041 cttccatcaa tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag
    32101 acgcgctcaa taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc
    32161 ctgtgatggg aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg
    32221 aagatatcaa cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat
    32281 tatctgctct tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc
    32341 gagtgatttg gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg
    32401 gcttgggtgg gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca
    32461 gcgaaatcat ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg
    32521 caaggatcgt tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag
    32581 cagaaggcgc tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa
    32641 tttacggaac accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca
    32701 atagtatttt agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag
    32761 gatttggtgc agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa
    32821 aagcgccgga tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa
    32881 tgaaaaaaga tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt
    32941 taaagcgcca tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg
    33001 aattcgtaac agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg
    33061 ggattcttgc caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag
    33121 caaaagctgt agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc
    33181 tagaagaaac gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg
    33241 gtgttgtttt cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg
    33301 acaaactgcc gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc
    33361 taggtgcacc ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag
    33421 gatttttagt agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg
    33481 ctgcattgaa tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat
    33541 tataatctac catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg
    33601 aattaatttt gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa
    33661 agcataatag gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat
    33721 gggtagctcg attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca
    33781 aaagataacg cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa
    33841 aagccccgtt tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt
    33901 gattcttcaa atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca
    33961 aaatttattt ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa
    34021 aaaccgggca tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca
    34081 gtttcaatcg aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat
    34141 ttaccgaact tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag
    34201 atgatctttt acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg
    34261 attttgtcat tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag
    34321 aactgattca gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac
    34381 aagatcttcc gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc
    34441 cgttatacaa taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc
    34501 tagcaaaaga aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga
    34561 tcatccaaaa cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg
    34621 aggttgaagc gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg
    34681 ggaatcccga tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag
    34741 aaaaggattg attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg
    34801 gatcagttgg tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt
    34861 ataccaggta ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta
    34921 gaagggaaga tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca
    34981 ttgatgatta aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt
    35041 ctagctggtg cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg
    35101 ttccagacag attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt
    35161 ggagtgattt gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga
    35221 cagaacttaa aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt
    35281 tattcaccga ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg
    35341 tcaatggaca gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct
    35401 tgatccccga accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt
    35461 acgaagatga agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag
    35521 caggtcatac gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta
    35581 cgatcaacgg cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac
    35641 tgctaatggt tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata
    35701 agacctcttt gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa
    35761 ccatcaatgc gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag
    35821 atgggaagtc ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg
    35881 ttgaattaaa actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg
    35941 gttatccgtt agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc
    36001 agtttctcca tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct
    36061 ttgaagcacc tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg
    36121 cattttaatt tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt
    36181 cctttaagtt tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc
    36241 ataagtagat tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta
    36301 aacattcctc ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga
    36361 aaatgcaagc aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta
    36421 cttatgagat tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa
    36481 cacgaggaat ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg
    36541 atattcctgt aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag
    36601 aagaaccgga acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt
    36661 tgattgatga tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg
    36721 attttggcag accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat
    36781 tgccaatccg tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt
    36841 tagtagagat gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt
    36901 agaggagaga gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat
    36961 atccacgatc gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg
    37021 atgttcggag caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta
    37081 gtcagttcag gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg
    37141 tacttaggaa gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt
    37201 tatccagcga ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg
    37261 atcatcaaaa agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt
    37321 ccagtcatca tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat
    37381 aatcagggca cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca
    37441 attttcttca acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt
    37501 attagcggat acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac
    37561 gctgcctggt ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta
    37621 tatcttggag caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc
    37681 ggccatctta tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt
    37741 cataagacgt tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct
    37801 cctgtaacaa gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg
    37861 tttgtgatcg gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc
    37921 gctttgattt taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga
    37981 gtaattgccg caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag
    38041 aaaaatctgt tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa
    38101 gtaggcacat ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat
    38161 cttatcttac cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc
    38221 aaatcagaac gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa
    38281 gttatgggct tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa
    38341 aagcgccagt actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc
    38401 ttcgatgtag cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc
    38461 tctgtccaaa aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta
    38521 gacgtagcgg ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa
    38581 acaatccagt gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc
    38641 ttgcttgatt tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc
    38701 atcgtaggtg atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt
    38761 ctaggcgcac aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg
    38821 tacggacatt atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt
    38881 cgggtgcaac acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag
    38941 gaacatgggt taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac
    39001 ccagcaccag ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg
    39061 cggatcgttc agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg
    39121 ctaaatggaa aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt
    39181 cagatcaata cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga
    39241 atcaaagcaa tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag
    39301 ggacaattga tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt
    39361 acatacaagg agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact
    39421 gtatgcgcga tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta
    39481 tatgacatca ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa
    39541 aatctgcgaa gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct
    39601 ttcacaaatg acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa
    39661 gctgcaaaaa acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc
    39721 ggcgttatgc acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta
    39781 accgagtctt ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac
    39841 catgtttgcc atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca
    39901 ggaatccacg taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt
    39961 cccgaagatt tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa
    40021 gctttgatcg aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc
    40081 catggattag aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc
    40141 gaaacagcgt ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa
    40201 caagtgatca attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg
    40261 ctaacggttg gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc
    40321 gataaagaag actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa
    40381 ggcgaaacac aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag
    40441 tatggaacgt ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc
    40501 agatgctagt gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac
    40561 tatcactgat caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa
    40621 ttatggggta aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt
    40681 aaaagaacat gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt
    40741 gaaacaaaaa ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat
    40801 ccgtgaagtc ggaacgatga aagctagtat agttgatgct ggagattcct tcgaacatgc
    40861 atttgatcag ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa
    40921 caaaccttat ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa
    40981 acatagtatt ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa
    41041 cacagatgcc gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc
    41101 tggtgatcca aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt
    41161 accgattttt ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac
    41221 ctataaaatg aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg
    41281 aagaattgat tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc
    41341 agaaaaactc atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca
    41401 cagagattat cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga
    41461 tgcactacat ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac
    41521 agatgccgaa acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca
    41581 ttggtcaagc cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag
    41641 aaggatatga agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag
    41701 tggcagatca agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa
    41761 aagagcgtcc agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa
    41821 tggagctggc agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc
    41881 tttctgcgat cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga
    41941 atcagccaat ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa
    42001 ataaaatcgg ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag
    42061 gtatgtgtga aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc
    42121 ccgctaccca atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag
    42181 tgatgagaga ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg
    42241 tgggtatcca tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg
    42301 aatatcaaat gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag
    42361 gctgtaatgt ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca
    42421 atccgcgtgt ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta
    42481 agctagctgc taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag
    42541 gaacgacttt tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct
    42601 ggccgtttga taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg
    42661 gtgaagtgat ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt
    42721 tagaaatcgg tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc
    42781 taatccaaaa agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta
    42841 gaagagggta caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag
    42901 ataaactgtt gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca
    42961 agatattgaa aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg
    43021 aaataaacga agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata
    43081 agatggttga cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct
    43141 atgaatatga aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg
    43201 gtcctatccg tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag
    43261 caatccagca ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga
    43321 ctgacttttc tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga
    43381 atgtgatcga gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa
    43441 tcaacttagc cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag
    43501 acttggatcg tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc
    43561 ctcaaccttt aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa
    43621 ttggttatcc agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg
    43681 ttgaaaacca gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac
    43741 atccagtact agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg
    43801 acggtgaaac agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt
    43861 caggggattc aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga
    43921 tcgaagatta cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc
    43981 agtttgtcat ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga
    44041 ctgttccatt cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta
    44101 ttttgggaga gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag
    44161 aagttcatgt taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact
    44221 tagggcctga aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag
    44281 ctttgtacaa ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat
    44341 ttacgattgc ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa
    44401 ttggctatag tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg
    44461 tcgttgaaac agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa
    44521 taagagatgg taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt
    44581 ctaaagacgg tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt
    44641 ctttagacac agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc
    44701 ctatttaacg gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca
    44761 gatgaaacaa gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga
    44821 aatgacattg aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat
    44881 tctcgtgccg cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca
    44941 cgaaacaaat acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc
    45001 tactctttct gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt
    45061 gtccaatctt catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc
    45121 tctatatgag ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg
    45181 ttttgccaca catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag
    45241 aatcgctact gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg
    45301 aacgaagacg gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt
    45361 ggaacagctg ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg
    45421 tgggataggg gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa
    45481 aagtgtgaaa gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat
    45541 gagaatcctt tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca
    45601 agcggctgtt tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga
    45661 tcgatcatgg tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg
    45721 gcggaaacac catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt
    45781 gttatgcata aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat
    45841 gttgctggtg cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca
    45901 aatgtgaaag caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct
    45961 tttggaacag attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct
    46021 gttcctgtct atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca
    46081 atcgaagctg gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt
    46141 gaccttaaga caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca
    46201 atcaagcctg tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc
    46261 attgggatgg gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca
    46321 agtgctgtag ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt
    46381 aaagagctcc ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa
    46441 gaagtgagag aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag
    46501 atttttcaac taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat
    46561 tgtttgtgaa aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc
    46621 taaaagaggc tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg
    46681 tcgaaaaagc aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg
    46741 ctggcggtat tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag
    46801 gtggaaaacg tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa
    46861 tgcatgccga tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct
    46921 gtgccaaaaa agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca
    46981 aagaaatggc aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag
    47041 tcgcaggaga tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt
    47101 ttatcgttat aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga
    47161 ttcaaacaga atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat
    47221 tagaaattga agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca
    47281 aaagcccaat ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga
    47341 tcgcaaaagg attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg
    47401 gaactgcaac agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa
    47461 tggtctatat tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta
    47521 tatttgaagg tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct
    47581 tggaagcagc tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct
    47641 ttacgtatga gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga
    47701 cgttgaccaa ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc
    47761 aagaattaac tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc
    47821 gcttgatgaa gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa
    47881 aatccaaaaa ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg
    47941 tgatcagttt ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag
    48001 aataatctct tctctttttt ctattatata tagattttct ctattttttg gtaagggagc
    48061 tgtttcaaaa atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata
    48121 ttcggaaaaa gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag
    48181 gattgaacaa tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata
    48241 cagttgcagc acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag
    48301 aagcgaatgg acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact
    48361 acacagagcg ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa
    48421 gagggatgct gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc
    48481 cagctgagtt ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag
    48541 aaggagaact attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa
    48601 tcatcaaaaa taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga
    48661 ttaaaagctt tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac
    48721 tattgaacca ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa
    48781 agaaagaaga aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata
    48841 tggatgtcga atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg
    48901 attggtttgc gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg
    48961 aagaaaccaa aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg
    49021 acaaggaatt caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt
    49081 tcaagaaaga acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca
    49141 tcaacggaaa acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga
    49201 atctagttgt gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag
    49261 aaaatgggga aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct
    49321 ttccggagca aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg
    49381 ggccaatcca atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc
    49441 ccttcgatgg tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag
    49501 aaatttatta tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc
    49561 tgtagcaaac aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag
    49621 tctttcagct ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac
    49681 gaatcgaggg gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat
    49741 attggtccag cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt
    49801 ttcgatcgct gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga
    49861 attcaaagac gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga
    49921 agaagtcaaa gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc
    49981 tggtatcaga agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa
    50041 aacgcatatt tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga
    50101 agaattgtta tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt
    50161 ttatgaagac cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg
    50221 tactttgatg aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat
    50281 tggagggatt cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt
    50341 ttatatcatt tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga
    50401 gcttactcgg ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga
    50461 acaccgttga tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta
    50521 gcaaacaatt tataactaat tgctgttgtg ggaaagtacg caaatatgat tctagaaaca
    50581 ctcattgatt acctgttttg tttcatagct gtaatttttt cttcttctgg tgtcacgata
    50641 atcgtctttt ggttttcata aataacataa ccaggcttgg cgccatttgg ttttcggata
    50701 tgctttacct gtacaagatc aaccggcact tgggcagagt atcggtattt tgaaaaatag
    50761 gcagctaact cggcagcttc tgtaattgtt tcatctgagg gcttgtcact tttaatgatg
    50821 acgtgcgaac cgggtatatt ttttgcatga agccaatagt cggttttttt agctgtcttc
    50881 attgtcagct ggtcattttg aagattgttt ttaccaacaa gaatgagcgt tccgtcagaa
    50941 gaaaagtatt gatcaggctg agatggtttc ttacgttttt gtttttttaa gttcttcttt
    51001 ttcaggtatc cttctgaagt caattcttct ttaattgctt cgatatccat tgggccggca
    51061 atttccagtt gagataagac agattcaaga tattggattt catctttggc ttcttggatt
    51121 tgttcgccaa tcagcttaac agcgttcttc agcttttggt atcgatggaa atatttctgt
    51181 gcattttgat ttggtgtcaa tgccggatct agtgctattt tgattggccg atcttcttca
    51241 taataattag gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag aaaagtagta
    51301 agtagttcgc catttctacg gtaattctca gcgttttcgg attctttgag tgtttgttca
    51361 cgttttttca atttattttt atttcgtttt agttcatttt cgatttttcg aatcagttct
    51421 ccgccttgtt gttttgcccg atctttctct gctttttcgt gataatagct atctaataat
    51481 tgactgagtg ttggataagc tgttacagca gatgcctgtt cggacaatac ctgataagca
    51541 atcggtgtga aaaaatcttt attttccgtt tcgtaaaaag taggaatagg ctgatggctg
    51601 atggctgaga aaaattcgtc ccatacaacc atcttttcat taggacgttc cagtaatctt
    51661 ttggtcagct cttgagcgat atcgaaaccg attccctgaa actgacgttg gattgctttt
    51721 ggatctagtt cagtttggga aagccgctga aagatttttt ctttctcgga gctaaatggg
    51781 ttcaagacct cttgttttgg cggagcaaca tattctacac caggaagcaa agaacgataa
    51841 gtgttttgag agcttccaat gtgtttgatc gcatccagta tttttccagt ttctcgatta
    51901 actaagacga tcgtactatg acgtcccatt aattcaacga tcaatatgat attttgtaga
    51961 tcacccaact cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt ttcgatttgt
    52021 tcgatgtctt ctaaaatagc gccatccaaa tattttcgaa gcatcatcac gaagttaggc
    52081 ggattatcag gattctgata atcgatttgg gtaatctgta cacgagcata acttgggtgc
    52141 gcagataata ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac gatttcattt
    52201 tcataaggct gatggatttt cgagatccgg ccagatagta acgtttcacg caattcgttg
    52261 accatcgcgt gggtaaatac accatcaaag gacatggttc attcctcatt tcttttcata
    52321 agtatattca ttataacgaa aaaaagaaga aaaaccaatt tggccgaata gtaaagcaaa
    52381 agtctagaaa aatcttggac taattacata aaaaacagtg tttctatcgt tgaaatccaa
    52441 atttttaggt
//
LOCUS       DACIRY010000004        47511 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.004, whole genome shotgun sequence.
ACCESSION   DACIRY010000004 DACIRY010000000
VERSION     DACIRY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.004"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            99..284
                     /locus_tag="IYE22_000162"
     CDS             99..284
                     /locus_tag="IYE22_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ9505410.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            351..746
                     /locus_tag="IYE22_000163"
     CDS             351..746
                     /locus_tag="IYE22_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ9505411.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            736..1113
                     /locus_tag="IYE22_000164"
     CDS             736..1113
                     /locus_tag="IYE22_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505412.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            1114..1509
                     /locus_tag="IYE22_000165"
     CDS             1114..1509
                     /locus_tag="IYE22_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505413.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(1816..2214)
                     /locus_tag="IYE22_000166"
     CDS             complement(1816..2214)
                     /locus_tag="IYE22_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ9505414.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2406..3173)
                     /locus_tag="IYE22_000167"
     CDS             complement(2406..3173)
                     /locus_tag="IYE22_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ9505415.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(3208..4431)
                     /locus_tag="IYE22_000168"
     CDS             complement(3208..4431)
                     /locus_tag="IYE22_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ9505416.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            5412..6215
                     /locus_tag="IYE22_000169"
     CDS             5412..6215
                     /locus_tag="IYE22_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ9505417.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            6800..7255
                     /locus_tag="IYE22_000170"
     CDS             6800..7255
                     /locus_tag="IYE22_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505418.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            7374..8432
                     /locus_tag="IYE22_000171"
     CDS             7374..8432
                     /locus_tag="IYE22_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ9505419.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            8474..10423
                     /locus_tag="IYE22_000172"
     CDS             8474..10423
                     /locus_tag="IYE22_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ9505420.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            10426..14067
                     /locus_tag="IYE22_000173"
     CDS             10426..14067
                     /locus_tag="IYE22_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505421.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            14467..14805
                     /locus_tag="IYE22_000174"
     CDS             14467..14805
                     /locus_tag="IYE22_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505422.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            14915..15136
                     /locus_tag="IYE22_000175"
     CDS             14915..15136
                     /locus_tag="IYE22_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505423.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            15156..17312
                     /locus_tag="IYE22_000176"
     CDS             15156..17312
                     /locus_tag="IYE22_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505424.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            17324..17557
                     /locus_tag="IYE22_000177"
     CDS             17324..17557
                     /locus_tag="IYE22_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9505425.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            17547..18413
                     /locus_tag="IYE22_000178"
     CDS             17547..18413
                     /locus_tag="IYE22_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505426.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            18428..20398
                     /locus_tag="IYE22_000179"
     CDS             18428..20398
                     /locus_tag="IYE22_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505427.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            20565..20792
                     /locus_tag="IYE22_000180"
     CDS             20565..20792
                     /locus_tag="IYE22_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505428.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            20789..21283
                     /locus_tag="IYE22_000181"
     CDS             20789..21283
                     /locus_tag="IYE22_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505429.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            21458..21793
                     /locus_tag="IYE22_000182"
     CDS             21458..21793
                     /locus_tag="IYE22_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505430.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(22050..23633)
                     /locus_tag="IYE22_000183"
     CDS             complement(22050..23633)
                     /locus_tag="IYE22_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505431.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            23755..24339
                     /locus_tag="IYE22_000184"
     CDS             23755..24339
                     /locus_tag="IYE22_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505432.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            24350..24595
                     /locus_tag="IYE22_000185"
     CDS             24350..24595
                     /locus_tag="IYE22_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505433.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            24601..25323
                     /locus_tag="IYE22_000186"
     CDS             24601..25323
                     /locus_tag="IYE22_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505434.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            25454..26197
                     /locus_tag="IYE22_000187"
     CDS             25454..26197
                     /locus_tag="IYE22_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505435.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            26203..26568
                     /locus_tag="IYE22_000188"
     CDS             26203..26568
                     /locus_tag="IYE22_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505436.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            26584..27114
                     /locus_tag="IYE22_000189"
     CDS             26584..27114
                     /locus_tag="IYE22_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505437.1"
                     /translation="MSQNISAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            27276..27644
                     /locus_tag="IYE22_000190"
     CDS             27276..27644
                     /locus_tag="IYE22_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505438.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            27835..28095
                     /locus_tag="IYE22_000191"
     CDS             27835..28095
                     /locus_tag="IYE22_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505439.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            28713..29513
                     /locus_tag="IYE22_000192"
     CDS             28713..29513
                     /locus_tag="IYE22_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505440.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            29550..29732
                     /locus_tag="IYE22_000193"
     CDS             29550..29732
                     /locus_tag="IYE22_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505441.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            29922..30209
                     /locus_tag="IYE22_000194"
     CDS             29922..30209
                     /locus_tag="IYE22_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505442.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            30300..30539
                     /locus_tag="IYE22_000195"
     CDS             30300..30539
                     /locus_tag="IYE22_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505443.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            30667..30996
                     /locus_tag="IYE22_000196"
     CDS             30667..30996
                     /locus_tag="IYE22_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505444.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            31049..31880
                     /locus_tag="IYE22_000197"
                     /pseudo
     CDS             31049..31880
                     /locus_tag="IYE22_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31895..32212
                     /locus_tag="IYE22_000198"
     CDS             31895..32212
                     /locus_tag="IYE22_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505445.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            32215..32447
                     /locus_tag="IYE22_000199"
                     /pseudo
     CDS             32215..32447
                     /locus_tag="IYE22_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32562..33038)
                     /locus_tag="IYE22_000200"
     CDS             complement(32562..33038)
                     /locus_tag="IYE22_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505446.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            33164..34495
                     /locus_tag="IYE22_000201"
     CDS             33164..34495
                     /locus_tag="IYE22_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ9505447.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            34520..35047
                     /locus_tag="IYE22_000202"
     CDS             34520..35047
                     /locus_tag="IYE22_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505448.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            35034..35597
                     /locus_tag="IYE22_000203"
     CDS             35034..35597
                     /locus_tag="IYE22_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505449.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            36015..36752
                     /locus_tag="IYE22_000204"
     CDS             36015..36752
                     /locus_tag="IYE22_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505450.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(36769..37185)
                     /locus_tag="IYE22_000205"
     CDS             complement(36769..37185)
                     /locus_tag="IYE22_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505451.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            37578..38825
                     /locus_tag="IYE22_000206"
     CDS             37578..38825
                     /locus_tag="IYE22_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505452.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSNPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            39095..41485
                     /locus_tag="IYE22_000207"
     CDS             39095..41485
                     /locus_tag="IYE22_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ9505453.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            41534..43369
                     /locus_tag="IYE22_000208"
     CDS             41534..43369
                     /locus_tag="IYE22_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ9505454.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            43444..44301
                     /locus_tag="IYE22_000209"
     CDS             43444..44301
                     /locus_tag="IYE22_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9505455.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNVNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            44291..45370
                     /locus_tag="IYE22_000210"
     CDS             44291..45370
                     /locus_tag="IYE22_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ9505456.1"
                     /translation="MKNKILLFLTLICTLVLTIYLANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            45709..46509
                     /locus_tag="IYE22_000211"
     CDS             45709..46509
                     /locus_tag="IYE22_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505457.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            46597..47403
                     /locus_tag="IYE22_000212"
     CDS             46597..47403
                     /locus_tag="IYE22_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505458.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
BASE COUNT        17568 a         6653 c         9091 g        14199 t
ORIGIN      
        1 gaaaaaatta actagcacca cgggttaaaa catcctcttt ttctattgat tccatacaca
       61 acaatgtgta taatagattt tgtaaaggga gggtagctat gcctaagaca gcaagagaaa
      121 ttgtaaaaat tctcaaatct aacggcttca aaaagactgg acaaactggc agtcatgcaa
      181 aattttataa ttccaagact aaaagaacgg tcgttgttcc gatccacaat ggtgatgtac
      241 cactaggaac agaagctaat atctggaagc aagcagggct ttaacagcct tgccttccta
      301 tttggtatta ggcatactat cctctcttac actagaaaag gagcgaggaa atgaagacaa
      361 aaaatatttt ttatccggca atcttcgaga aagaaggaga agcttataac atcgttttcc
      421 ctgacttacc ggaaatatct acctttggaa atagtcttga agaagcatat ttaaacgcta
      481 aggatgcttt aggattagcg ctatatagtg taccagatgc agaatttcct aaaccatcag
      541 cagtggaaga attaactttg aaagccaacc aagtggtagt aatcatccaa ctaaacatta
      601 aacttttccg aagaagctta aatacaaaaa caattagaaa aaatgttagt gttccggaat
      661 ggttagttct tttagggaag gagaaaaaca taaacttctc tcaactactt caaaaagcac
      721 tagaagaaga gttgcttgaa aagtaaaaag gagaccgaac ctatgaaagc tacacactta
      781 aactatttct ctcgaacgtt aatcaataat ctattaaaag atacaactgg cattagcgtt
      841 agtaccaaac actttgccaa tgatctaatt atttttgagg ataataactt gcgaattgag
      901 atgtttattg aacaggcaac ccctaactca atacctgaat tctgctatac gcacactgaa
      961 aaaatgaaac atgcaaaatc agttaaagat gttgaagcat taagtgaatc actaacgaac
     1021 gctttaaacc tcgttaagaa ggcaaaattg ttatttgaat ttcaaaccga ctaccacaat
     1081 gagttgcttg ctcaatctga aattttagat taattgaaac aggagaagag aaagatgaaa
     1141 caaacacaaa cagaaaccgt taacgatacg cttgctgatc aattagcagg ttattataaa
     1201 ttatttgaga tgggtaaagc acagcgcttg atccactatc tttggaaata caaaattttg
     1261 tttctttttt gggaatcaac actaacattc tcagttcttt tttataagtc agatttttat
     1321 aatcaacact tagttttagg gagttttttg cttatctaca ccattttgat gcttaattta
     1381 tatacctaca tcacacttga agaacactta gtaattagcg atgcagggat cgagagaaac
     1441 atcaaaaggg tatccttcaa gatcacgtta aacaagccta ccgataaaaa gctctcagaa
     1501 gcgttctgag agcttttgac attttcttgt ataattttat cctaacacat tacaaagcac
     1561 ctaattcgag cttatatcaa gcaaatttgc tatttattac tcaatacata tctaaaaatt
     1621 acttgatgga aaattagata catggtaagc atgaaaagaa aatcaatttg agcagatgca
     1681 gtacaataca tagcaattta aaagctctca gaaggcttct gagagctttt aatatattct
     1741 ttgataattt gtctttctta aatccatacg tcctaaaaca agctgttttt gcttaatatg
     1801 atcatctgag agacatcaaa tcaacttttc tctcaacaaa tcattcaaag ctttcgacag
     1861 attaatattc tcttcagccg ctaaaacatt taaatagtgt tccacagtca ctgattttcg
     1921 atcataattt tgcttgtttc gagctttagc aactttcgta ttagctttta cgacaaataa
     1981 attaacattc tcagatcgct tgattcttgc atttaactct tgaacaggcg taggcgatgg
     2041 aatttctttt tcttgcaact ctaaaagcac caagtatcct tccaatactt cttgcgccat
     2101 ctcaaaagca tcttctaaag tttcaccatg tgtatataag tctggaaaat ctgggaattt
     2161 aactgcatat tcatttcctt tccattcgaa tactgcacaa aaaaagtatt tcatcgcatt
     2221 gcacctctct aattaaaaaa taatttttgc ttttttgttt caaaaaaaag aatcgcctac
     2281 caaaaaaatt gataggcgat cacaagctaa ttaacctcta aaggagatta ataccttgct
     2341 cccaaggcac tatatatctt agcatacgtc tgaagcatct tcaaacgagt aagatatatt
     2401 ttgcattaac ctaatttttt ttctctataa gttttttcat caaactcact agaaatatta
     2461 aactggacag caaaattgta atttctgcct tttctaactt tagcaaaatc aatattcagc
     2521 attgattttt cattaatttc ctcaactgat tcccttaaaa cacgtctatc aaaattcgat
     2581 attgttttat actcattttc tgttttcgta atctcacgaa ttttaactat ttcaatcagg
     2641 ggattcttat atatttcagc tttctcaaaa tctcctttat ttttggcgat aagatatttt
     2701 ttataataca tcgccaaata tttgtataac acgatgctat attttttttg aaaagatgct
     2761 aagtcttcaa aaacaaagaa cggatacttc tttttgattg agacaattaa gggaattaca
     2821 tcttctgaaa aactgaaaga tacactatgc tcttctaaat cccaatgaat ccgatcaata
     2881 gcagatacag tagtatacgt cttatatttt ttgtctagat tcgaaatttc ataaactgct
     2941 tgtctttgca aagtgttaac tatggttttt agttccgttt caagattttg atcaacatcc
     3001 atgtaattaa taatcgcttt aacaggtaaa tctactacgc ctgttttctt tagctttgtg
     3061 ggtgagatat aactgaacag caattgataa agttcatacg actttaaatc cattttcatg
     3121 tcaattgttg ctaagaactc ctcattgtac tttgtcagtt catctttttt caaaagtttt
     3181 tccttccttc acgcattttt tcagttgtta atcataaagg ttcacataag gcaaataact
     3241 cgtaattgat ttttcgagat atttaacagt attttttatt gtgtaatcct ccttatgagt
     3301 agcaacgtaa gataggtgat tgacaactga ttcaaatcct ctatctttgg ataaacgatc
     3361 ataaagcgga taaactttat tctgaagtcg gctcataagt ttaagatcgc ataagtcggc
     3421 agaactgatc aaaaagtttt tgattagtaa tttagtgtat ttgctattca tagctttcaa
     3481 gtacgattga ttcattttct ctgttttatc ttctgcgcct tttaaataag cagggtctct
     3541 ttgttcttgc ttataaggta ccggaactga catggtacgt gtaagcaaga actttatgtt
     3601 tgtataagta tgacccttct tgataagctc atagtcaatt ttaaaatcag tgtatttatt
     3661 cagttgggca atcggttctt taattacacg tctttcaaag tctcgaaaag ctttatattt
     3721 tttctcaata ctaaatttct ttcgtaaatc tgcaatggag aaaacaggat tttttaatct
     3781 atcaagtgat tttgtactcc atgttaagtt atctctattt gcttcataca gattaaaatc
     3841 tttagtgaat aattgaaaaa aactgatcgc atacttgctt gtcaagttaa ctacttgatc
     3901 aaatgtatat tgcgtaaaat gattttctaa gttcgttaat aaaggcataa ttgaactact
     3961 aaatttaata gatactgcat cagaaaaatc attgaattca gtaatttcta gcggtgagat
     4021 gactttgtat tcagtcttac cgttaggttt agttctttga attttaatga cagattgctg
     4081 atgcaatttt tcaataactt gttttaatct tgaatgtttt gaagaagatg aactagaaga
     4141 catcaacgca aataaagcct ttttagatac tttgacggta ttattaaaag gcggattttt
     4201 agggtcaaga tgtcctatag cgtaaagata aattttttgt ggcatagcat ccatttcaac
     4261 aatgctatct acaagttgat tcgataaagt aacagttaaa tcaaactttt ttagattgct
     4321 ttcggtagtt tcaaatttcg ggtttacatt cttttctgtt attttcatac gattgcctct
     4381 tctttctaag cttggtacga gcttaaaaac ttggaactgg tacgattcca ttgaataagt
     4441 atagacctgt tttcgatacg ttacaataag aaatgtatcg taaatttata acataactca
     4501 atgtcagtaa aacacatttt attttttcca atcaaatttc attgaaaata cagcagaaat
     4561 cgctatttta gaacattttc aaaataaaaa tccttttcaa taaaatacga aattataaaa
     4621 attaccaaaa ttaaaaaaca aatatttttc tagtttttag aaataaattc acatactttt
     4681 tctttttttg gatatattcg gaataccaca tcgctttttg tccacgtttg catcgctttt
     4741 tgtccacatt cacatcgctt tttgtccatg ttcgcatcgc tttatgtcca ccctaaaatc
     4801 tatttaagat tgatataaag cctttttgga ggttgctaaa gttttaaaga ttaaaatata
     4861 tattataaat ataaataaaa ggggaagctg aagcttcctc cttttccttt ttttcaatta
     4921 ttcaaagtta cacaagcaaa ataaactcct agatagtgtt agtttttttt aagatttttt
     4981 atttttaaga ttttgaaata agagaggagt aatcctcctt tattaaatat ctgttaatat
     5041 atctgttaaa tatctgttaa tatatctgtt aggggaggct taatctcttg ggagagtaag
     5101 tattaaaggg gtataaaggt aacacttttg gacagtaaag gtaacaaaag tggacagtaa
     5161 gggtaacagt tttggacagc aaaggtaaca aaagtggaca tttgaggtat cacactagga
     5221 taagacgtta ccttcggttt tcttgatttg gaaggcgaaa aaatagaaaa ttagattagt
     5281 aacaaatctt tgttgactgt tacctttagt tgtgatttaa tttaggtaac aagcagaaaa
     5341 aattgtttcc tttaattagt accagattaa catgattaaa ggagcgcaaa aagaagattg
     5401 gagagtaatc aatgtctcaa gaagttaccg aaaagattat tgatagtgat gtagatttag
     5461 tatcagaaca aacaaatgca attgtgcgtt ataacaatgg tctgaataca acagctttgc
     5521 gaggatttaa gcctgttgaa atggatgtgt tttgggctat tttatcaaaa atgaaacgtc
     5581 aaggaacagc agaagtaact tttgatttca acctctttcg ttatttggtc aaatacgaca
     5641 gaagagaaaa ggacagtttt tattctgtgt taaaagattt gagtgacaaa ctaggtaatt
     5701 taacctaccg ttttgaagat tcagacgtct atgaacagct gtggcttttt caaaaattcc
     5761 ggatagataa acagaaggaa acagttacaa ttaaggcaaa tgaggaattt gaatttatct
     5821 taaactcaat tggcaaaaac tttactcgtt ttgaacttga taattttaca aaactaagaa
     5881 gtagctacac aaaagaattg tatcgtcatc tcatgcaata cagaaataaa gagtggcgaa
     5941 atggttattg ggtagtcaaa gttgaagatt ttagaagagc attaagtata ccttcaagtt
     6001 atagaatgac aaatattgat aaaagaatac tagagcaggc gaaagaagaa tttttagctc
     6061 ctgatgaaaa cggaatacag gtatttgaaa cttttgatta tgaaaaaatc tatgcaaaga
     6121 aaggaagaag agttgatcgg ctcagattca cttttagtga gccagaatca aacttgccaa
     6181 ccatttcaat gcataattgg ttagaagaag attgagaaga agcaggttgt aattttaata
     6241 caacctgctt ctttaaaaaa gtatgggtaa cacttttgga caataaatgc aagaagtttt
     6301 tttgtaagca agtttcagtc taaataatat ttgaaagaat ggctaatcta tacaaatttt
     6361 aacaactatg ggtaacactt ttggacaagt caagaaaaaa aacaattttt cttaatttgt
     6421 tgatcgacta aaggtaacac ttttggacaa cacagaataa aaagtccggt gatatcagtt
     6481 ttggtaggaa acatcttaat atctttgtga gattgaaggt aacatttttg gacaagtcaa
     6541 ctatttgtta aaagttaata tagagtaatt agataagttt tttcttttcg tctgataaat
     6601 ggataatatt agttatttta ttgctaatta gtctttcatg atgactgaaa tagctaaaat
     6661 aaatatctcg ttggataatt gctgatactt taagggatat ctaaatttgc tgattgattt
     6721 atttatgtta ttatgatatt ataataacat aaatgtgtta cgcccgaacc tcattttaaa
     6781 aaaatgaagg ggtgagacaa tgaatgctat taattggcat gaatttgtta actatatgga
     6841 gagcttacta gatgatctaa aaagttttag tattgctgat gcagtaaagc atgggttacc
     6901 agttagtaca acgatgaaat tacgaggatt gaaaattcca gatgaaatta ttaagctgag
     6961 aactaaaaca attattgaaa ccgcaaaagt cgttgacaat atgaaccatg aactaaaagg
     7021 caagaaaaca acttacgaga aagattttga acgtttttta aaatctgtta ttggaaaata
     7081 tctaggtaaa aaatctttca gtgaattgag agatgaagag attgtaccta ctccaatggt
     7141 tagcaaaatc cgaaaaaaca aagccgaggg gaattactat cttggtacaa tagcaaaaat
     7201 cattgattat ttggaaaata aagagcaaaa gaaaggaacg aagaaaggaa gataattttt
     7261 gtgttaaata ttaatgggga agaatatacc gaaatgattc aaaatcccga acctagaatt
     7321 accgaaaaaa cgtctgattg tttgctcttt tatgaaaggg ggcagacaaa ctaatggaaa
     7381 aaaacgtcaa ttcaacatct accaaagatc aatacagtat tattggaggg ttgcttggtg
     7441 ataaggataa gagaacacgt cacggattac gcattatgct tgcaactttt ctaggagtgc
     7501 tttttttatt aacaggcttg ggtagctaca tttatcaatt aggaaactat aattattatc
     7561 tcgtgaacaa tacaacacct ataggctctt cattggagtt tgctcgatca ggagcagaga
     7621 tcactatttc agatgtgtgg acagatcaaa acagagatgt aacagtagta aaactcggtt
     7681 actcgcaaaa cgctagaaag ttgttatcta cgcaaggaaa aaactacaac ttatatatgg
     7741 caacgaaaga aaaagatcgt cctaaaaagc ttgaaatgag ctatgggatg ttaagtactg
     7801 atggggatgc tttcttattc ctaaaaggta aattagatga aagagcttat caagttttta
     7861 ttgcaaacca agtatcacta atttctggtg atgatacagc tgacgatgat accaaagtca
     7921 atgtgaatga agcagattca gttacaaaag cactttctca atattctatg aatgatgtta
     7981 ataatgaagg agtaatttct tttgggaaaa aagcaaaacc tgttaatact cctgataata
     8041 tcaatttcag aataaatcca tattcagatt caactaatgt ctatcaagga agctttctta
     8101 ccaaagatgg cgatattgat tattcaaaag ttgtagcagt tacaagtaag aataatgttg
     8161 taagtaagtt agaagatcag ttaaatgctt caaatactaa catcaagaaa attgaagcta
     8221 caaaagctga gtatctggaa cgtttacaac aaaatcctga cgataaagat tcgcaagaaa
     8281 gtattcgtca actagacagt cgaatggaga ccgaaattaa agcaatcaat gatacacaaa
     8341 aattgattga tcaatacaaa aatgctgact ttaacaaaga ttcttttgga gacatgaaga
     8401 cgacatttaa gtacctaact ctgactgact agaagtaaat tattcttatt ttagaggaag
     8461 tgaacacaaa cagatggaaa atagtaccga gttttttaag aatattcaaa ctgaatttaa
     8521 tcatgctctc acaccaatga aaccaccgaa acataagtta gtgggacgtg atgacgaact
     8581 ccatgagttg tcactgatct tgaatagacc ggaaacaccg caagctatct tgttaggtga
     8641 agccggaaca ggtaaaacag cacttgcaag tgaatgggct gaaagacagc gacaagatgg
     8701 aaagaaagta caagtataca aattatcaat cggacaattg cgtgttgggg gagatgatgt
     8761 cctccaaaca cgtttagaaa cattaattcc aagattgaaa gaatatgagc gaattttaaa
     8821 atctgaggac aaagaaaatg aatttgtcct cttcattgac gagtttcata aagttgttag
     8881 ctcttttggt agtggcaata aaattggggg tgattcgcta aaagaaccgt tagcaaatga
     8941 atcacttcaa ttgattaccg ctactacaag aaaagaatat gaaaactata tcaaaagaga
     9001 tgatgcctta gggagacggt tacaaccgtt agaattgaat gaactaaaaa gcgatgttgt
     9061 cgtcgatatt ttgagacaat ggctaaatac atcctctgtt aaagatgatc ctcaggcttc
     9121 ttttaaatta tcgcaaagga ttcctgatga attacttcaa gaaatcgtac aagcaaacaa
     9181 gctttatcgt gaggatttgt ccgaaccggc taaatcaatt gccgtaatgg aggatttgat
     9241 tgcaaaagat cgagtagacg gaaatgctta taatcgagaa agtctattgc aaatatttgg
     9301 taagtataat attcagctag attctgattt tgatatggaa aaacttcaag aggaattaaa
     9361 gtccataaaa gggcaaccgt tagcagttta tactataaca atgttgttta aacgtatgaa
     9421 gcttggattt gatcgtttta aaggtcgaaa gcaaccaaaa gcaactgctc tgtttacagg
     9481 atcaacaggg gtcggtaaaa ctgcaatggc taaagccgtg gcaagaggac tttatggcga
     9541 tgaaaataaa gttctcttag ttaatatgcc ggactacaaa acaaaagatg atgcaatgcg
     9601 ttttagacga ctcataggta ctcatgtaag gcatgatcct tctgcgatta ttgtgtgtga
     9661 tgaatatgag aaagctggtg tagaaacact cgatgccttt ttatcaattt tagatgaagg
     9721 taggataagt tttactgaaa gaggatggga tgatcgagaa gttcacgaca ctgtttcatt
     9781 gtcaaataca attttatttg cgacaacaaa tgcaggtgcg gagatgttcg attcgttaaa
     9841 cagatatagc tcactatctc gtgttacaaa caaggaaaat tctaatgaat atagcactga
     9901 actaaaaaga gaataccgaa aagtcgcatc agggatcaaa caagccttga ttggtgaagg
     9961 tctaaagcct gaagttatta accgatttgg acgaatagtt ccattttggg ctttatccga
    10021 acaaactctt ctggaaattg cttataatga aatgttgaag agtgctgaat tagtacgaaa
    10081 acatttaaat atagatttac gtcttccacc taggaaagaa agagtatatg gtcaaaattt
    10141 ttatgcagac gatctctcca tgcacttagt ttttgacatg gcttcacaag aggattctaa
    10201 atcagggggt gcaaggaata tcttgcattt agttgaatct gagtttgaag aagcgattac
    10261 tgaagcaata attacttatc caaacgcaaa aatattcgat gttaagttga gtgaagccgg
    10321 agcgtttgaa ggaaaaggtg attctaatgc tgaaattatt gcaaagcctt ccgttgctga
    10381 aagtaaagaa aacaagattg tattttcgat tggaggggcg tagctatgaa attcaagaaa
    10441 tatttactac aaaaccttag aataaaacga tgggtcgtaa gaggtctagt tattggagga
    10501 gtattagcag ttactttagg cgctcctttt ggaatgaatg atccaactcc tgacaatcaa
    10561 gatagcaaac aagaaacagt tataaataag aacgttgcat atgcggaaag taaaccggca
    10621 gaacaaggaa tgtggacact atatgctcgt atcctaatgg gagataaaga agttaatgat
    10681 agtgccgcta aaactggaca aatgaatttg ttaggacaag gtggtatcag tgccgatttc
    10741 ccttattctg acatttctaa acaaggtaat tcgattgcag ggggtaatgc aggtactcaa
    10801 ctagcatcta ctttagcaac ttatagttac tacggatata ttgaaacagt ctcaggaaat
    10861 gctattgcca gtcaagcatc atccattcta ggaagtattg gtaggacgat tggtagtttt
    10921 atcggatcaa ttagtttaat cctttctaat attgctgaat ccataaatga actccttgga
    10981 aaatttttca tgagtatgaa tatattttct cttatggggt ggggagaagg tggatcggat
    11041 gtatctaatc cggtatctaa agcattaact agcttgctta acaatttggg attaaataat
    11101 gaattattta aagcgatagc aagcttatct atgctctttt tcacagcaat actaggttat
    11161 caaggaatga aattattgtc gaaagatggt gttaactcga agggattatc accagtactt
    11221 gaaaaatgga ttgttcgtat ttttgtattg tttatattct taccagtggc aggatcgcta
    11281 ttgggagttt atatcaacgg catccagtca gcaaaaaaag atggagatat accaacaaat
    11341 gttgtttctg actacatcat taacacaaga ctttgggctt ctactcaaaa tcttgctcca
    11401 tcatctgata ctggtggtga tgtacctgac ggaaagccta catccgaaat caagttcgtt
    11461 gatacagatt atgaaccatc taaaaaatct gcaatgataa gagcgatcaa tactagcagt
    11521 tatgccagac aaaataatga tctagattct aattcagtcg gtatggctct attaaaagaa
    11581 tggtcatcga atgatacttt caatattaat acttatgccg gtgatgttaa tgtcgccgaa
    11641 aatgatgctt atatgggaag agcgcctgct gtttggacag gctataattt aggggatgga
    11701 aaagaaggaa gtgcaattaa gaaaccaacc aatttaaatg gatatatttg gtcagcaaat
    11761 caaaatgtga cagacgacaa tcgcaagcct gatggaaaag gaacagcgca taactttgat
    11821 gctactcaac cttggggcgt tgctaacaat gcaactttta gcacacagtc ggtagcttta
    11881 ttgctccaat cggcacttga tacatcggga gctaagtttt atgcgtataa tatcgctcct
    11941 tcaggaattc aaggcagtgc taagaatatg tcaacagtaa aaacagaatg gcgtacagca
    12001 tccttagttg gtcatggagc tatgggtaag tttggctcgt ggctagcgat gaacgctgaa
    12061 tcaatcgttt tagcagttct ttacttagca tgtgcttggg cgatgttcaa tgtgaatatc
    12121 gtgaaagcat ttaaacgtga atttattcgg atttgggaag tagttctaac aggatcactt
    12181 gcatcaggag caggtgtgat ggcaatttca ttaggtgttg ttacaacatc tctgatcgga
    12241 ctacaattac ctatctttct gattactttt attcgtgcat ttggtgatac tgctgtggga
    12301 ttcattccta atacattagt tggcgatggc gtagcaaata ttgttcgatc tgctatgatt
    12361 ttaggtgtag gatatctttt agtctttaaa aaaggcgaaa aagtaaataa tttgattaat
    12421 atgcttattg gcttaccact tgaagtagcg tttgggtttg atgaacgtgc taagttatta
    12481 tttggtaata gaaatgatgc tgtgcgagtt gtggacggcg tttctaaagc aatcaagaaa
    12541 agccaaggag ctaaaaatcc ctattatgat ggtggcaaca gctcagtaaa agatatggtt
    12601 gcaggaggtc ttggagcagt tggagcgcaa aaaatggctg aacgtttgaa caatatggga
    12661 gatagcaatg ctgatgctaa gtccattgct gataaagtaa ttcctgaaac accagaagga
    12721 atgtcattca acggtggcgg agttggttcc acaatcaata atacctcttt caaagattta
    12781 gttcgtcaag atcaactacg taataactcc caacaatata ttgatcattt aggacgtatg
    12841 gttgatgaaa acggaaactt tgtcgatgaa aatggtgaaa tattaaaaga tagtaaatac
    12901 ggtaaagatg ctaaaggcaa ctttattgac gaaaaagggc gtttggtcga tgaagatgga
    12961 aaccttatag atactaatgg taaaccattg gaagatagtg aagatggtaa tattggtatg
    13021 gatagcgaag gacgtttaat tgatgaaaaa ggtcgcttag tcgatgaaga tggaaatctt
    13081 gttgacgaaa atggacgcag gttgctaaat ggtgattttg gtcatgatag taacggacgt
    13141 ttagttgacg aaaaaggtcg tttagttgat gaagatggaa atcttgttga tgagtttggt
    13201 aataaacttg gtaaacacag cgatggatat aatgtgaatg gagatttagt cgatgaggat
    13261 gggaatatca ttgagacgag agatgcgctt gtggcagatc gaaatagaga actcgaacaa
    13321 caacggcata cgccaattgc tgttgcaatt cctgagaatg aagaagacga gattacttta
    13381 gatcaaactc atcaagaact gaggaatgaa atgagtggtg aggacgtgtc agacagagtg
    13441 catcgggaat taggagatgg agcatctggt gaaagtcagt caagagagct taacagcaac
    13501 tttgataata gtgctattcg gaatgacaca gtacttaaag acagagaatc gctaacaaaa
    13561 gaaacggata aagaacaatc agatgtgaag aaaaagttca ttcctactcc taatgttaat
    13621 caaacagata aatatgaaac aaatattgga tcaggaatgt cgaaagacaa tcaatcaatt
    13681 agagaacctg aactgacaaa taaaaataat agaacaaata attctaaatc tacttcagat
    13741 aattcaggga aatctaatca atcaaccaag aaaacatcaa cagctaaatc agccgttgaa
    13801 tatctatcaa aaactggtaa aaatgtttac acctcagcag ataaagcttt agcaaaagct
    13861 ccaaaaggta gtctagcagg gataacctat gaagctaaaa acgaagctat taaaataggt
    13921 aaaggaacgg ctaaggttgc caaggctact gctacaggag catccaaaac tggtaaagta
    13981 gtgaagaaat ctaccatgtc agtcaaggac tatattgtaa aatacggtaa tcaaaaatca
    14041 actgaaaata agtctgaaga cgagtaaaaa gaaggagcat tgatttgctc cttcttttta
    14101 ctttgatttc taactgaaga tgtcaaggaa aatttctatt tatgcaaaaa aattatttta
    14161 aattgtctat aaatgaatta aatatttact gttcaaagtt gtataattaa aaagggctta
    14221 tgctggtaaa gaacagaatt attatctaaa caaaaacttg ataaaccagc atttttagcc
    14281 aataaaaaaa gtgaaataat ttaatggaaa atctctttta atcatcgaaa atttatgtta
    14341 ttatgataac ataaagatgt aaatatcgag acgtcttttt cttagttaga tcattgacaa
    14401 gtgaacaaat aagatttgtc gatgatataa ttaacttatt aaaaatatta aaaaggaggt
    14461 ttttatatgt tcgattctat taaaaatttg gttagcggaa tgacgcctga tatgaaaact
    14521 ttagtacaag ttgttttgta tttcttggca ttcgtttttt cgttgaaacc aggattcact
    14581 gcaatgaaat tttttggtga tgcaaaatgg ggagatggat taaaatatgt tggggcgatt
    14641 attgcaatct ttattattcc tgtaattaca accgttatgc tttacacaat gggtactaaa
    14701 acaggtaatg atttgaacca aaaagctaat atcatatcag cacttattcc agttgttggt
    14761 atctatctag tttcaaaatt tccaaaaaat gaactgttag gttaaaagtg aattgaacaa
    14821 atttggataa tatgccgatt tgttcaattt actttttacc aaaaaactac gctccaagcg
    14881 tagtttagaa gtgagggaag aaagtaaatg taagatggtt tttgatagag aaaaaatgtt
    14941 agctcacgcc aacgaagtat taatgagcag tctgaaaggt accgaactgg caaagataat
    15001 gaatatgaat gttaaccagt tctatgatta tcgaaatggg agtaaaaaga ttgaaaaagc
    15061 cagacttgaa acgcttatta aattcgaaaa agcatatgta tatatgctag ataaacagaa
    15121 aagaactatt gactgaaaaa aggagtttta caataatggc aaaattcaaa gatattaccg
    15181 aatctattaa ttatgtggca gaaacagttc ctgtagttgc actctctttg aagccacaat
    15241 tactcgaagt gtacaaaaaa gaagaaaaag aagcgaaggc aaaaaaagct aaagcaaagg
    15301 tatggaaaga attcgatgcg ttgctgacca aagatttacc aaagaaaaat tatatggtaa
    15361 ttgaagatgt acaaaagctc aacagtgagg gagttataga tgaggatgat gctattctcg
    15421 attctgagaa gtatgtcgtt ctattgaagt caagtttgaa ttatgtagct gatctaccag
    15481 aaaaatttaa agagaagcta ttagcaggtg tccgttttat ggatagtagt ggtcaattat
    15541 tagttattcc ggatgatcct aatgtgtttg aatcagaagt tgaagaagct acagacttct
    15601 atagcgcagt agagcaatta gaagtagcta ctaatggatt agaacgaatt gctgagacct
    15661 tcaactcttc tgaagaagtt gaatttgaag ccgtagcaga aaatgtacca gatgaaaaag
    15721 aagctctttt tgtggatgat aacaaaccaa ccgaagcaga aaaccaagaa gtaagcatag
    15781 aagaaacaca aatacaaaca gaaacctacc aacaaccaaa aattgatgaa aaacccgaat
    15841 cccaaagtga caaagaaaat tatgtttatg aagctgaaaa aatcacatct gaaagtgatg
    15901 taccgaaaaa cttagatgct gattttttag aaaaaaattt tgaacagatc attgctgaca
    15961 aatttcaacc tgtagtttta gatgagatta atgaccctgt cgatttaaac gaggagatgc
    16021 tatccatacc tgagttgaaa ccattgctgt ttttagctaa agagagtatg aacgctcgca
    16081 tctctattgc taataataaa atcaacagtg aattaaataa gacgaaggag caattgatcc
    16141 tcaaaggaaa gcaaatgtta gctgatgagc ttttgcggat agaggaagca acctcattaa
    16201 atggtcaaaa tcagtttaca aatacactat cggttgctaa agagagctac gaagcagaag
    16261 catctaaaat tgatgattta gtgatggaaa aatccaatat cattcgtaaa gattatgata
    16321 tggcgaaaca ggcttatttg gatgctgaac taaagaaact agagcaacgg tatgatgctg
    16381 agcatttacc agaagttaaa caacaagaag atgaatatag aaaagcaact gaagaaagtc
    16441 ttgatcaaca gtaccaagaa gcaattgaat ctattaaaaa cgatgcattg aaacaaagcc
    16501 aagaagcaaa agaagttgtg gtggaacgaa ttatcaattc attgaaacca gaaattgaaa
    16561 aatcattaac agacgtgcaa gaaaaaactt tagagattgt cgaaaaaaca aagtcagaaa
    16621 atgaagctga gttccatgat acaaaagaga aaacaacaga tgtggttgtc aaatattttg
    16681 cgaacaaaga agaagttgaa aagcaggtag ctaacaacac gaaagaatta gaaacaaaac
    16741 ttgtagagca acaaaaaact atcgaagaat ataaagatcg aatgaaagag gtcaaagatt
    16801 tagcagaggg ttataaatta aatagcgaaa agttggcaga acaaagtgaa gctttaagag
    16861 aagacttatc tcgttcccga agtgatctag ctcaaatggc agaaaaaacg aaatcattag
    16921 cagttgctac agaaaatcaa tcaagttcaa aacgtgattt tacaagtaaa aagatggcta
    16981 ctatcatttc tgtcgctgca atggcttgcg gaacgatctt gggatcgtcc tactttatta
    17041 gtcataataa tcaacaagca caggctcaaa ctcaaacatc tatttcgaga gtggaaaatt
    17101 cgattgggac aaatacgaac tctgcttcaa caaatagtag ctatgaagca acaccaacta
    17161 cagagttcaa agttgggaaa gatgttgtca tcagtattga tgggaaaaca actaaggctg
    17221 aagtgatttc tattgatgga gaaaaagcag ttgtgaaaac tccagacgga aatcagtaca
    17281 aaattcaagc tcacttatct gataataact aaggagggct attatgcgag aagaaaaaat
    17341 agtttatcca gtaattcaaa acaatcaaga aaacatgacc tcttctcagc acttacagga
    17401 agcacagctc gtgtatcagg taaatgaact accacaaacg aacgagacaa tgagcaagtc
    17461 aatatcttta attggcttgc ttattgttgc aatagtgata actgtaggtg tttaccgtaa
    17521 gttgaacgtg aaaggaagta tttctgatgg cagataactc aaactatgga tttaaacata
    17581 acttacatca aaaaccttct gaaacaattg tcagagttga atggcttgaa aagcatgtca
    17641 atagcaccta ttctgttaca gcaaaaagat taattgtatc agctatagtt gttgcagtcg
    17701 ttgttcttgg atcaattgtt tacttagttt ctgatacgat taaactgaaa tttatttttg
    17761 ttccaacgtt attaatgata attggatttc ttattctaat tagccagtat atgtttattg
    17821 atccatctag tggttcattg atttctggtt ctattagact gttcaaatca gtagttaatt
    17881 cagcaagagt atctatgggg aaaaagaaac catttcgagg cacagagatt ttaaaagtga
    17941 aacaagatgg aacaatcgta tatcgagatg ggagtgtagc attaatgttc aatctagatg
    18001 gggctacatc actaaccgct tttccagaag aagttatggc tcaggaatcg aaatcaatac
    18061 aatatcaaaa taccagagat cgttccttag ctgaaatcaa aatcacttca tctcaacctc
    18121 agaatacgga aagacaaact cgaaatatga aaatacaaaa acgaaatgca aggagtaaca
    18181 aagcagtctt tgacataata gatcagcaag aacgatttgt atcagaatat attgatggtg
    18241 tgaaaacaac agtaatccaa tatcttatct tgaaatctca atctaagatg gtattggatg
    18301 aaggtgtgga acgtttagaa aactatacga gtagaggact ttattatagt gtcactcagt
    18361 tgtcagctaa acaaacaaaa gaaatattat ctgatattta tgcactgaaa tagaggtgag
    18421 agattaaatg gaaattatgg gtagatcaca actattaaat ttcaaaaaaa agagaaaaac
    18481 taaaaaagaa caaaaaacta gaaattcaaa ggtagtgaat aatacctttg ttagtgattt
    18541 aacatcttta gttggttatc aatttacacc atcttacatt aggacaggta agcggtgggg
    18601 aacaattgtg aaattagtga atacttatgg aatgaataga aattatggat ttggttggat
    18661 ggttaaagct attcctgaag tgagagtaga aggggtaaaa gcctatctaa ttgaagcaga
    18721 taagccagtg tcacaaaaag atcaacaaga aataatgcac gagaatgttg ggggatcaac
    18781 gaatagttat gaaaataatt tagaaggaaa aaattcagat gctgaaatta gcaaaactgt
    18841 tatgcggatt atggatttga atacagcatc tttactagac agcatggcaa ataaaattat
    18901 tgattggaag ctagaaatcc tattagtatc cgattctcct aataaaatca gcgaacagct
    18961 aattaaactc caacggttgt atgatgaaaa aatgtatgga ttaaagctta tgtcatatgg
    19021 aggagatcaa cgtgatctgt ttgatagact tttaagtgaa ccaatcggga atgtctatga
    19081 gtatacgtct atgagttcgg attttgcagg aaatgatcat gcaattcgca aaggtttaga
    19141 tgatgcgaat ggatgggcta tcggcactat gtctactagc tacgcaagtg gtgtagcttt
    19201 tatggcactg gatgattctt ttacaagtaa agttttagtg gcatcacaca aaaacgctac
    19261 aattaaaggc tatgatgaaa aaatctcagc atcatcactt tgggggcaat tgattgctaa
    19321 taatgcaatg gcaaatcata aacgaacttt tcatattgtc ttaaataaat ttgattactt
    19381 tggtgatcca actggtagaa catttgttgc caaaccttcg ttaaacagtg taatagaaaa
    19441 ggttaatttg gctgaagaag gtcttaactc tttggagctt tttggtaatc aaaaggacat
    19501 tggtgattta tatagacgga atctcgataa actaactcag attttttatt tgttagcagg
    19561 cagaacatta gattctgcaa ctagagtttt attgaaaaaa gaattaggta acttttatat
    19621 aagtcagaaa ctttgggata ttaattatga agaacgttca aaacttttgg tgaacagtag
    19681 tgatacaatg ccagttcttg gggattttat tagctatttg actaatgctg aaacaaaagc
    19741 caaaaaatct ggtactgaaa aagaagtcga tcgtattatt gtattaaaag acacattgag
    19801 aacagcatta gacagcttct ctaggatgtt taatcaaaga acgacactga aagaaatcgg
    19861 taaaagtgac aaattacagt tttactataa cttaggtgat ctaagagcag aaaccaatgt
    19921 tatggaagct caatttttaa atacctttga tttcatcaca tcacaagcaa aaaaagatga
    19981 tattgttatg attcacggct tcgataagtt atctattgaa accgttgggc taattaaaaa
    20041 aagattaaca atgcttgaag aagatggaat tcgtcttgcc tttctatttg atacaatcgg
    20101 ttcaggagaa gacaatgaat acaaagaaga tattgaaaca gctgatatat tcaatacaga
    20161 tggaaaaatt taccaaagtt ttgaaagtga ttttaattac actatcattg gaaagatgac
    20221 cagtggagag ttaatgaagt atgaaaaaaa agttgctcct gctagaaaag gagaagatga
    20281 tcaaaaactt tctgttgatt tgagacagta tttgacaaca ggaagaagta ataaatttca
    20341 gatcaggaga ccagtggatc tgactaataa ttttgttatt ggagagttta ttctttaagt
    20401 tttatctttg attttgagtg tataaggaaa ggataagaaa aaacatgtat atttgagact
    20461 aaaaacagtc ttagagaaca tggtacgttt tgtgaaaaga gaactataga aatttcataa
    20521 agtgaaacct tattgagtga aataattata gtggaggtta aataatgaaa ccaaacataa
    20581 aaattttaga tcgaatattt ctaggtagag acacagaagt aatcctaatt caacatgaag
    20641 aaggatttga agttagtata ggtattcaga aacttcaaaa acctcattat tgtaatcagt
    20701 tgtataaaaa ctttactgat gaagaaaaag cgagagtttt ttttaatgaa gtcaaaggta
    20761 tgagagaaca gtatgaggtg gttgaagcat gaataagtta ccagaaaaga accgtgtatt
    20821 tagattgaaa ggtgatctca gtcaatttaa aatagatgag aatggacaat tttttgtttg
    20881 gatgcgacat gagcattgtt ggtgtaatcc ttttgtgtta aaaaaagaaa atgaaatcag
    20941 aactaaaatt gcaaatggtg agtgggatta cctcttcaga tgggaagatg aatcttttat
    21001 agcaaatgat atggatcatg catttgtctc atttctggat aatacatggg gtacttggta
    21061 cgattgttac ctagaaaaat tgggctttca aaatattgat attgatgcta ttgaatgtct
    21121 taaagaagtc gatcaaaagt atgaagaaga accctattat ctcttgcaac aaaaattagc
    21181 caataattca gtgtttcagg tagaaatttg ggaaaatgaa gatggaactt atgaggggct
    21241 agcgcaagaa atacccttct cagataggaa gagttcgatt tgaaaagagt atatcagcaa
    21301 tttttatgaa gtgaaagcaa gcttacaata tagtaatgga aaattgtggt tagctggaag
    21361 gtatattcca gctaaccaaa tcttacgttt tctgggagag tggcgtttta aaacaatttc
    21421 ctaggaaaaa tagttaacat aaaaggaatg aattgttatg aaacaatatt taaatgaatg
    21481 gaaagcgata gaggggtcat tagtaacgca aagaataaag gaattacctg attgcttaga
    21541 gaaggatcat ttgtttcaaa tacgagaaat gttgaaaaaa gaacagtttg atccagacca
    21601 atttttagta gtcgagtatc caactattgg agtgtactgt tgtaaccata tcaatgatgg
    21661 aaaatatttt attattcagg agtatggagg acagttaact cctttctata ctacatggga
    21721 aatgagtgaa gaaggaatca ataattttcc atgtgaaagc atcgaagatt caatttcaca
    21781 caccgaatgt taattgatta taactataaa aaaggattga acattatgtt caatcctttt
    21841 ttcaatgaaa tgttttccgc catttcattg tttggtgcga tttctatttt attatatcaa
    21901 aataaagaga gattctaaag aaagaagagg gaaatatata aatcaagcga cagtatattt
    21961 attattaaat gctattgtga gaatataact aattagttta atttgaaagt aaatatacgc
    22021 tataaaagtg catcttgtgt tataaaaaat tattttattt tgtgaacata atttaacaat
    22081 tctttctcgg aattgatttc ttttcttaaa tagtcttcag acttattaat aatattatct
    22141 attatgtcta gatacatatc acttttcgat tcagctaatt catacagttt ttttaattct
    22201 ttatccttta attgaaaacc ctgatccata gtcactgcat ttggatatag aagcccacta
    22261 taatgtttac gtaaattcac ttggaatttc tgattataaa agtctaacag attagaaata
    22321 aaaggcggaa aattactgtt tttgtaaaat tgattatttc catttaggtt gtatagtaat
    22381 ttgttaaatt gctcctgatt taatttattg taaatccatg gtaatacttc catatcctta
    22441 tctgctaacc tgaatgaact agcatcggta agatgttcga tcataaaatc tatgaacatc
    22501 tctttttcac caaaatataa atattgatta tatattgttt ctaatgttga agatggtagc
    22561 gaagaaactc tcattttttg ctggagtata ggatcaacta gactatttaa gtgattttgt
    22621 aattcttcat tgttgtttcc tagaactgca agggaacgta aacggtagta atattctatt
    22681 tctgttagta cttgttgttt cggtttaaaa tcatttattg atatttgtaa attttgaatt
    22741 gtctcgtctg aaaaatcatg ttgtaattct ggatgggtag ctataaaagt aagtaaaaag
    22801 aacccttttg tatacgtgat ctgttgattt tcaccaagtg atttattaat ttcctctctt
    22861 gattcagctg tgaacctata ctttgcaatt tcttttttta gaatatttaa atagaattct
    22921 ttattatcaa aaattagggc attaagtagt ctgaaagtac tttccctatt tctatctgct
    22981 tcctcatttc cataagataa ttgaaaaaca aacttaaagg ctgtcttaca taggttttct
    23041 ttggcttttt cgcttagttt atttagatag ctatttttta gtgcagagtg aaattcttca
    23101 ggtgtaacag acatagcatg tatgcgactt gcatattgta taatttcgat cgttactttt
    23161 tttcctaata ttggattttt taggaatata atttcatagg acttccgtat aacatcctga
    23221 caagtttctt ttgttatatc tcttaattcc caagtattac cgttgtaagt gactacagga
    23281 tgagctgaat aatttctttg atccttaata tattttaaat cctcatattc atagctatca
    23341 attagctcag ttgaactata agctttttct agtagttcca tttcccattt tggagagttt
    23401 ggatcacttt ttcttagtct agctatatct tctaaaattt tcttagaagt attatcctcg
    23461 taaatatttg ctaaatcaga taatttatca attaaatcta aaataagcgc actgtaattg
    23521 acagctattg cagaacgata atttccctga taaaaactac ttctaacttc ttctaataat
    23581 ggaataattc taggattttt aacttttttt atttcatcat ctaatataaa catataaatc
    23641 tccttttttt atttgtatga caagtttacc ataaaaaaaa attaaaaagc gagacatatt
    23701 tatgttgttg tgttatcata ataacataaa tatgtagagt gagatgatga aaaaatggat
    23761 ttgaaacaat cgttttacat ctggttatat cctgataatc ctatgtcaat aatggctgat
    23821 atttttgtag ttagcatctt attgtattta ttttatctaa ttttctttca caatttaatc
    23881 gggtacatga ttcttgattg gaaaggaatg ttcgatatag aaagaaaacc atttgctaac
    23941 aatcaaacct ataccgtctt cgaaagtgga gattgggaag aaaatgaagt tgatgtgatc
    24001 catgaaattg gattagcttt atcaggaata caagatgctg atgttttggt atcaatgaca
    24061 acggatactg gatacaacgc ttatgaggta ttttatctta tgtattttat atggcgtgat
    24121 ggtcaagaaa ttatcaatgg tcgtgctgaa aaaacaatca attctaatac tgaaatttct
    24181 ttttgtatgg gtgaagggga aatgattgta tataaagatg ggtcagcaaa cattaaaaca
    24241 gcctttggtg atattgaaac aagtattaag gaacttaggg aatatgtgca gaacccatat
    24301 aattttcaat atttagcagg aagaataaat ttagtttagg agtaaaaata tgaaaaaaat
    24361 tataaatcaa ccaacaaatc acttgataat tgaaggtgtt attttcgtaa caatgtattt
    24421 tactgtagga aatggcatct caaatacaat cattcaaaaa aatccacaat acctattatt
    24481 tagtctttat gcttggattg tacaaatggt ttgggtttcc atattgatgc tgttgtttta
    24541 ttggatagta cgggttattc ttttattgtt aaacaaaaga aaaaaggata ggtgaataaa
    24601 atgaaatcaa ctaaattgaa ggttgtattg ttatcgacag cagcattaat aactcttaca
    24661 gcatgtggta caactaatag tgctgataat aacaaagaca caaagtctag tagtacggtg
    24721 gttactaaaa acagctctac atcggcatca acaacaattt cttcaatgac tgaaaataat
    24781 gctgaaaagt atcaaaaata cgaagacatt aataaaggtc aaaagccgga agaagtttat
    24841 accgacttta ttaatacagt aaaaaatgat gaagattatg ggaatgatcc attgcaaaat
    24901 aaaaatctga ctatcgttct ttacaaacct gattgtccag attgtcaaaa agcagaatca
    24961 aaaatcatgc aagaaataaa agaaggattt agttatgctc caacattaat gcatgatatt
    25021 accataaata aaccagctta cgagtatcac gcagttgctg tagatattac tgatggtgta
    25081 cctgagtgga tacaaaacat tcctggtctg gctgatgaga acaatcaata tcatacgcca
    25141 acagttgcag tagtaacacc aaagaaagtt aatggcacac tatactggga tgtaatggga
    25201 atgacaacaa atacaactga tgaaatgatt gaaagtacaa ctaaatatta tatggatttg
    25261 ggagcatata aattatatca acagtcagaa cctgttatgg acgatcaatc caaagttaaa
    25321 tagttcttta gggcgtaagc cctatttttt taaaattaag tgataacaaa taaatgattt
    25381 ctgaaacaaa cgttcttact caactactcg aaacacacaa gctaattaac tagattataa
    25441 aggagaatgc gtaatgaaag aaatcagtgc aaatgaagct catattccgc aaaagaatca
    25501 ggttggtata tataaggtgc ttcatggtga aggaatttat aaaatacagt tacttaccgg
    25561 ttacaataac ggtgcaaatg ggaagtggtt aaaggcaaaa agtattcgtg aacttaaaac
    25621 tgaagtagaa aaaataagac ttgggttaac aagggaagaa ataagtagac tgagtgaaaa
    25681 ttcttctttt gtaatccaaa aagaaaagga gaaacatcta cttcatcggg aactaataat
    25741 tgaaaaatac tacgatgttg atttacctga tcagttaaga attattgacg aattttcaga
    25801 gaaaaagtgg aaatctgaaa tatacccaag cttaattcaa tacaaggcat caggaaagcc
    25861 catgtataac tacacaggct ttttaaacta cacacaatat aaatatgcct ttcgcaaatt
    25921 aaaaggcatt aaacaagctg acattgccct gtatgagatg aaaaaaatgg atagattgaa
    25981 attgattcaa gagttagtgc aaaatacatg gaatcaagcg attgtcgaac tagaaaaaga
    26041 aaatgggaaa attttaacta atgaatatct gatcgtaaat gaaattgaag aattaattgg
    26101 tttattttca tgtgaaaatg gtgaagtctt cttgaagtca tcaccaatct attcaatttt
    26161 ggacagtccg tatcagttta tacaggtaag gagatgagtt acatgtctag cttgaaagat
    26221 gactatatcc aagcaaaaca agagtatgaa aaaatacaga tgttaaaatc tgagaaagtc
    26281 gaattatttt taacacaaaa attagcagag gatcatattg ctaacttaga tgctaaaact
    26341 ttagaggaat atgatcagca gtttaatcag acacatcaca atatcatttt gcttgttgat
    26401 caggcaaaga gaagacttga aaaagcggaa tatgaactat tagatcaagg aattatatgc
    26461 ttggataaag ggattcgaag atattatagt gcagagttaa aaaagcaaaa tgtagacaga
    26521 catgcgttaa aacatgaaat cttagaattg ataatgcaac gaatttgaag ggatagaaag
    26581 taaatgtcac aaaacattag tgctgtaaaa caattaatag aaaaagattt agagacgaca
    26641 gttcagaggt tagccgaacg aatccatttt gcaaattatc aaaccatctc taattggtca
    26701 agcaggaaca gaaatccgga agaaattcca atgaaatttt tttatcgaat cggaaaagaa
    26761 tatggtgttg atccgataaa aattttcgaa aagaccatga tttattacta tcatggaaca
    26821 cctaacgaag tgttggaaca tcttttggaa gaagcagaag aaaaatcatc aatattagag
    26881 atatcacaaa atgacaaagc tattcaagtg aaatgtgttg atccaacagg aaagattatt
    26941 gttggatttg atattgaaag aaaatctaaa atagatttta ttactcaatt aacaaagata
    27001 aataatttaa atattttaac acttagacca gatcatgaac agttgtttat gagttttaaa
    27061 aaacaattga aagaaataca aaaaagatat aacaaggtgt tttctaatgc gtgaaaagct
    27121 aaaagaaatc agagtgtttt ggataattaa caaagaattt attatcttgt atacacttgg
    27181 atttatcagc atgatcttgt tttggtggtt atatttaaaa aagtaattta aaagtgtcaa
    27241 ggagattttt atccagagaa ggatgtggaa tttgcatgtg gaataataaa ttaaaaacag
    27301 gcttgttatt aatagttatt tcctgcgcta tgatggttgg catgataata caaagggaac
    27361 aatcttattt tgaagtttct gctaacaatg taatagaaag atgccattat ggtcaacatt
    27421 tttggactgg ggaagtcaga gaaaatatag atcgtgagta tgttcaaaga atcgtttggg
    27481 atgcatattc tatcaaagat tatcctaaaa gtttaacgag tagattattt tacagcgaaa
    27541 aagataatca aaaactctcg aatttgatga tgaaaaaagt aaggaaactt gcacaaaact
    27601 atttggaaga aaaagaaggt aatagtccaa ttgacaaaaa ttgatgacac tgcaaaaaaa
    27661 tatagagttc gaattagctt ttttcggagc attgtttttt aggtgaacta tatttcatca
    27721 ctaaaatcag agataactaa cttgaaacat ggtcagatgt gttacttgaa gtctctgaat
    27781 aatcaatgtt atctagtaga attaagcaac taaaaaagaa agaagtaaga aaatatggca
    27841 tacgaaaatt tacaattagt agtcgcagaa gttagtgggg atatatacct tacaaaagta
    27901 aaagatggaa ttatggacac gaattatagg agagttgcta cagatgatgt gctaagctca
    27961 tcgacagaat ggtttcgaaa aaatgaagaa tctgctgttc actttgaagg aattgatgga
    28021 aacattcata gtttgttcta tacaaaagac aaagaaaaag caaaaaaaat tcttgatatt
    28081 ttgaaaggaa ggtagttaat taaccaatga tataagagaa tgtgagacca agtaaatact
    28141 tggtctctat aattttagcc actagtcaat taaatagcta aacaatttta ataataataa
    28201 aatatgactg cttcataatt aagaagtgca gaatttttct aagaacgata tttccttgtt
    28261 gaaatgagta ttgctaacaa tttgatcacg aacagcatcg ttacaaatgt tgtatcaata
    28321 tttgatagat tacatattct attaaaaaag aaaagaaaat tattgatatt atacacatta
    28381 gtgtgtataa taaaagagag ggagtgatta gaactttgag caattcacta attgtttaat
    28441 aatagaacga ttcctaagaa gttcattgtt cattggcaaa ttgctttatc agattgaaag
    28501 ttggcgaata aggtttgtag aataacacct tttcaagcaa agcgattttt gaaagctatg
    28561 aaatttgttt aataaaaaaa ttcaccagat aatctgatag agactaaccc aattactaac
    28621 agttgtaatt ggtgttagct tacaaattaa ttattgtgtt tagtcaatta caaattgtga
    28681 aacggcacta ataattggaa aggaaaaatg aaatgtctaa agatttacga gagcttaaat
    28741 tgcgaaaaaa gcaatataat caatattttt ctagtttgtt gaataaacat attgaagaag
    28801 gacttgatca tgattttgga aaagatgaat attgtttata ttgcgaacgt tacaatgaaa
    28861 aaatgtctga atatctacag ctgaaaaaag agatacagaa attagagaaa aaagtacatg
    28921 cttggaagta taaagattcc aggtctggtt atgttgaatc tcaaaataag ccaagaaaaa
    28981 agaagcaagc gtatgttcta aagatggtaa atggggaaga gttaattgta ttagcgtaca
    29041 gtgaagaaga ggttcgagaa ctcgttgaaa cacctgtcag gaaaaaggtt gaaacaataa
    29101 atgagataga cgacagccaa tacaatagca tctatattga aacaagtcat ggtggatata
    29161 agaaattagc aactttcctt agaagttcta aagttgggat aatttctaaa aaagacataa
    29221 gtgctgttac cgatagtttt tgtactttat ctgttcctaa agcttatttc caatacgata
    29281 gtaaaagatt tgaacgctta gagaaattct gtagaccgta tactgtattg aaaactaaaa
    29341 aatttaataa aactaaaaga gtttcgatac agttaccgac tggtcaaatc tcattaggtg
    29401 aaaaagacta tctgataaaa gtaggaaaag agttaattat aaaagtaccg gaacacttat
    29461 ttaaagaaaa ttttgagata cgtgaaacct tggtatggaa agaagaaaaa tagtttggaa
    29521 gacatagaat ggtataaggg gaaattgaaa tggcagtaaa taaaatattg caggtattaa
    29581 tcatagacga atttggcgga ggagaatggt ttgatgtgca gtccatagac tttgaagaag
    29641 aagtcgtagt tatagaaaat atttacggcg aagtatactc atattcgctt gaaggtgaga
    29701 atgttgagga tctacggttg attggtgagt agaaaaaagc agaaatatca aaatggagat
    29761 aaaaattaat agcatgtaaa gatagtcatg cttacatgct ttatccaaaa tggtatgcag
    29821 acaaaaatat agctgaaata aacgattatc taactagtat aaaggaacgc aatgcaatta
    29881 attaacaata gacgaaaaat gaaattaaag gagataggaa aatggtagaa gttgaatttg
    29941 gatcaaaaga atggtttgaa gagttaaact caatgattaa gttcccagaa aagtttactt
    30001 tttactacaa taaaagagac aatatcattc aagtagcttt acttgaaact aagggaagat
    30061 tagaaaatac tcgatggagt atgataaaac catcggcact tagatttcat cctaaagact
    30121 tgacagcggt tgtagcggta aaagaggttc aagactcaca agaatattat gaagtagtaa
    30181 aaaatctgaa ggatatttgg tgtaaataat tctaaattga ccagttattt ggtcaaaaat
    30241 agtatgtgat tatttcatcc aatgtggcat cataattggt atggaagggg ataaattatt
    30301 tgaaagaaat agaaggtatt aagaaaagaa atcaggaaag attggaactg ttaaatgaaa
    30361 atagaaatag aatgtttagt tattatgaat ctatgaggtg tttaaataaa tctagaagtg
    30421 aaattataga atcatcaaag tataaacaat tgatggaaga gagagtttct ctcatccaag
    30481 aaatagaaac agagtctatt tttccagatc attctcctaa agtccgattt tataattaaa
    30541 cattaacaaa tgaaacgatg ctaaataggc atcgtttttt atctatttat aaaattaatt
    30601 tatttacaaa atgtgtatcg gtatgtatgg tgaatttgta gataagttga gaaagggtga
    30661 tcttaaatga aattagaagg tagcttacaa aatagaatga tggaaggcag tacaacaaac
    30721 aaggagattg tagttggaat gggagcaacc gagcttctat ataccgacag aaatccttat
    30781 acagtaattg aagtaaaaag caaaaacaga attttggttc aagcggatgg agcaatcaat
    30841 aaagctactt ttccagatca agagtgggaa tatagcagaa atcctgaagg tcaaatattg
    30901 gagttagtta agacaaaaaa tggatggaaa gtgcttaaag aagatattca tttttatata
    30961 ggtgatagag aaaaatacta cgatcctagt ttttaaacaa cctgaagatg agcgaggttt
    31021 tatcctcgct cagaaaggag tatggaggat gttcagtcaa gaagaaatca tcagcaaatc
    31081 gcaaccggat aaaaatggaa ctataatcat gaagaaaaaa atcccttgct atcgttgtag
    31141 cggatcaggt cagtacaagt ttatgtctat ggggaaaatt acctatggca catgttttaa
    31201 gtgcgatggt gatggatttc ttgttgtaac tgaaaaatcc tttacgccag aatatagaac
    31261 taagctaaat gttttaaata aaaaaagagc tgatcgaaaa ataaggatcg caagagagaa
    31321 agaatctcag aaaaattttg atcgttatgg ctttgaaaag ggttatattt acgtggttat
    31381 agagaaaaat agctatacga tcaaagatga actgcatcat caaggagcta cttatgatcc
    31441 tttattaggt tggttttttt aaggaaaaac aagaacaata caaaactcaa aaagtttttg
    31501 tagaagaatt ttctgtaatc aatgatgaag gaattattga gttgaagcta gaagaataca
    31561 gcaaaagaca acaggagatt ttaagtgcga agcgtagaga agagggtgag tttattggta
    31621 caatcgctca acgattggaa ttggatttga aacttaccaa taccgtcacc atagatacac
    31681 aatggggcaa tgtttacctg catttatttg aagatcaaaa ccataattca ttgatttgga
    31741 agacaaataa atttctttat tatgatgaat gggatgttgg agaaataaga aaagttaagg
    31801 cgacaataaa agcgcatgac tactacagag ataccaagca gacttggatt aaaaatgtta
    31861 aaattcaaaa aaatgagtaa gaagggataa aagtatgaaa agagaagaat acaaacaaag
    31921 attaaacgaa ctgcttgaag aggacgaaac attaactcat ggttcgccgg atgaaattct
    31981 atacatgatt gataatatgg taatttttgg tggatatgaa cttggtaata ggtcagttga
    32041 tcataatatt ttagaatttg atgatgtatc atgggaagaa attcttgatt ggggaatttt
    32101 ggcagtacct gaaactaaaa cctatattag tgacactatg gttccttttt ttgaggaatt
    32161 ggattataaa agacttccca aaaatgaaaa tcatatactg ggaggaaact aacaatgtta
    32221 agtgacttac ttattttact gatctatcta ggtggaactg aaattattgc gtattttttg
    32281 cttgtaattg gtgaatatgg tatttccagt gcaatcactc aaaggtttag attaccagta
    32341 agtgaactaa aaaaatgatg agattatcag catatctttt aatgtttttg aatgtggtca
    32401 tgttaatctt tgtagtaatc ggtgtgactt tcaatttaac ggaatagagg gaaatctata
    32461 atggaaaaaa gagaaaagaa tgaacatatt ttttgagtca atattgttga tggtttttgc
    32521 gatcagcgct taccagaaat ctaaaaaaga gtaatgactg tttagtcatc actctttttt
    32581 tgtagggaaa ttgaaatatg tggagcttgt attggtttag ttaggattgc ttggctataa
    32641 ggagctagtt ctttatcaaa accatcaaag taggttttca gtagcgattt aactgatcgc
    32701 tcatcttgtt tgatttggat aatttcagca tccaaatcgg ttgtgaggtc aggagctttt
    32761 gaagcgatta cctgattttt tttatcttcc aactttctta ggctgtttaa caagttgtaa
    32821 aaataatcat tttgactgtt cattccaacg tgagatgcaa ttttaacaga agtttcatcc
    32881 gtattggcat caagtatttc atcaaaagaa ctcattggaa tatcaagtag taacgaggat
    32941 gtttccttta cagcattttt agtttttcca tcttttttgc tcttaacttg agatattttt
    33001 ggatctagtt taggtaaatc acttgtttta tcagtcattt attagctttt cctttctatt
    33061 aatttatgtt attatattat cataataaca taaaaaagaa cactttttgg gggcgtagtt
    33121 attgaaaaaa tctaagagaa agattgccac gttgcttacg ttaatggtgc agtttacagg
    33181 gattgcaatg agtgtaatca caataaatcc gcaagtgtta catgctgatg ggatagctac
    33241 ttatgatgca caatttaact gtactactga aggtagtggg tcaggaacag atagcgatgg
    33301 aaacgatact ggaagtggcg ctgtaacagg tgatgtagcc aaaaatattg aagatattta
    33361 caaatacatg aatggagaat atggcttatc aggtgctagt attgcaggta ttttaggtaa
    33421 ctggaaacaa gaatcaggta ttgatcctcg aacagtagaa gggtattacg gcgatgggca
    33481 gagagaagtt gacttcatca aacaatttac tgatggtcta atgggtattg gctatggtca
    33541 atggacagcc gggcgacata cagcattagt tgattggtct aagaaaaata atggcggttc
    33601 ttggtggact acagaagccc aattggactt tatgttcaaa ggtgatgggg gattcgtagg
    33661 aatattaaaa gattatgctt taaatgcttc tgatgatgta gtagaaaatg cagtaacttt
    33721 tcataaaaac tgggaaattt cagctgattc cgaatcagct gtaatagctg tacgtggtga
    33781 aaatgcaaaa gaaatctgga agtacatgaa aagcaagggt atggacggtg caaaagatga
    33841 aagtaagatc aataaaatat ccggaggttc aagtaccgga gatggatcga ataattcatc
    33901 aagttcagca tcaggaacag tagaagatgc ctgtgcaact acggatgatg ctaattctgg
    33961 ttctggatca attgggaaag aaggaaaaat taacggtaaa aaaggtcaag taatagcaaa
    34021 tatgacttat gaagaagcat taaaaaaata tgggaaagca atcacgctac caaaatttaa
    34081 agaaccagac tggtcaacat caccttttca aggtgaatta caaggacaat gtactgaatt
    34141 aacatgggct tatatgagtc aattatatga tggaaatcaa ccaactttgg gaaatggcgg
    34201 ttacgtttgg gaaagctaca aatcggcagg agcaaaggtg acgaacaaac caattgtagg
    34261 ttatgcgttt agtgctttag atggatggtt atgggctgtt ggatcagcag gacatacagg
    34321 tgtagttata gcggtgtttg acgatggatc atttttgtgc gctaatttca atgttccacc
    34381 tacccaagca ccaacaagag gtgtttcagt aactttgatt gatggtgtgg atgggtcaga
    34441 taatgtgcat ttctttagcg gtgtaggcaa agctaaattt gaaattgaaa aataaaaatt
    34501 tgaatggagg gcgacagaga tgaatagtaa aacgaaagat gcaattatca tttctttgct
    34561 ttctggtatg ctattagcaa tgcttgttgg aattctatat ttcaataagc agaatcaaga
    34621 catagaagct gaaaagcaca gtgtgcaaat tgaaaatgaa ttgaccttct ttaaagaggt
    34681 taaaccaaat gaagtggatg gattgcaaag agatttagaa aatgatattc aagagatgtt
    34741 taaaggatca gaccgaagca aatttgcgaa tcagtcaggt gaagaatttt tgcgaaagtt
    34801 atttattgtg acaggtaagc caccagttac taacgagagt agcgagcaag aaaaaataga
    34861 atactataaa ccttttactt ttaagctaac aaactttgga attaagacat ctagtagtgg
    34921 gttgtatgaa gcattgactg atttcgaagt tcaatacaac ggaaaaaaag tgtatgatac
    34981 cacattaacc atcacactaa ataatgataa gaaagtgact gggggtgctt tttatgcaaa
    35041 atcatgaaat tactactgct aaaaaggcga tcggaatact ttccataatt ttgatccttt
    35101 gttgtgtggt actatttcaa aagaaaaatg cttatgataa taatgtcaaa gaattggaag
    35161 accttaaaag taaacaaatc gaagtagtca agaataacca gaaagtgaaa gctatgcaag
    35221 aagcccaata caaagaattg ggaaatgctg aaatcaagga acaaagtagg aaattcttta
    35281 atttatttta caactggaat tcatgggaaa aatatacgaa gaatatgaag gaaatacaaa
    35341 aagaatttcc tcagatagta aagtctagtc ctgttgatat ctctggcaca atggtaggta
    35401 ctggtaatag tccaatatct agctatactg ctgattacta tacaacaaat caaaaaggta
    35461 aaatggttga attgattaca cagacaagaa atactccaac aacagcaaca acaacaattt
    35521 ggcgtggagt aacaagcata ggagaaaatc aattgtttta cttggaggat ttagatagct
    35581 atgtaagagc agagtaaaat aaaaaaagag ctaacggtac caacgttagc tctttgtgct
    35641 gacgatgata acaaaaccta ccgaaaaagg attgtttcat cacaaacaca aacagaaaag
    35701 aattgataaa aaacccgaac ctcagaattt ttattaatca attcattttg attataataa
    35761 attgagctga acattacaat acgtatatgt tgtaaaacgt aaaaaaagtc aaaagtaaat
    35821 actttcttaa aaagtcaaat aaaactatta aagacaatat atatggttta tttgacataa
    35881 gaaaatggtc tagatgataa ttagaatata gaattgtaga cttaaataaa tcataaaaaa
    35941 ccttctatga taagttatag ctcttaattt taagcagttt tagatataag aaagtaaagt
    36001 gggggagaat aataatggca gatcgtaaat tagaaaagtt attagaagaa acgtgggatc
    36061 caaaagaatt tagtgaattt tttatggaga attttgaaac tgacttagcg gtgattgtta
    36121 aagatgctct tagagagcaa gggtatcctg aaactgcaaa ttatataaac attaatttta
    36181 ctttgtatac tgaaaataga ggaacttggg atttttgggc taacttagca aataaggagt
    36241 taagtgataa atcagataca ggaattcgta acttttttga atcaaacaga gatgattata
    36301 tgtacgctaa caatcaaaat aaattgaact ttagagtaga gtttgatgaa acacctgaag
    36361 aatttataga aagacaaccg cctaaagaaa atgtagcaaa ggtattggaa gatcgttgga
    36421 attcagatga aatagtttca acaattagcg aacaagacgg acaatatgaa ccgctagtag
    36481 aagcggttag agaagagcta agactgaata agtttcctga tgttcaaaat attgatgttt
    36541 ctcaaattga aataaacgtc aatatcacca atagacttga ttatgatagt tgggcagata
    36601 tagcattaga aaaatatatt tactcaacgt taaaagagtt tatcgaaaat agaatgaata
    36661 tcatgtatct acagcatcca cagtatttaa actttggtgt tgaaatagct acacctcttg
    36721 aagaatggaa aatggagcaa gggcttgact agcctttgct ccattttttt attttacaaa
    36781 tgaatacagt tcttcgtctt taccaagaat gattgtttgc ccaactttga gattatcagg
    36841 tgatccttca gcggtttgta aggagaacgt tccattgaac ttatcggata ccgttatagg
    36901 tagctttaat tctgatcctt cctcatcttt gagaattgct gttttccgtt catcaaaata
    36961 aacagtcttt attagataat ttttagtagt ggtttcatca gatttgtctt tgtttttagt
    37021 agtactaatt tctttagaat tagatgtatt ttctatgact atttcgtttt tggtatttgc
    37081 gcttgtattt atttgattga aaattttaat cgacaagatc ccactaagaa tagccaacat
    37141 agaaacaagt gtaattaata ctgataattt cctttttttg ttcatttctt cttaacttcc
    37201 ttttttgttt tgattagtta gatattcctt atctaataag cctttttagg ctattaaatg
    37261 aagataaacc ccaaaaaatt attgcaataa tgatagtgca atggtactat tattataaaa
    37321 taaattttgc gaaaggggta ggtctaaatc gcaaataaaa agaaattgaa cctagacaaa
    37381 tacaacttgt ataccgcaag ggaagcaagt ctaattttgg gtaaaggtag aaattatgtt
    37441 acaaacataa tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt
    37501 gacaaaacct atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat
    37561 aaaaacaaaa agaaggaatg aatatgaaga aaaattcgct tgctatggtt ttatttgcat
    37621 cagctgtact tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa
    37681 acacaccgga tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag
    37741 atactatttg gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg
    37801 agtttgccga attgaacaat gttgatttgg caaaaggaga acaacggact atcccgattg
    37861 gacgaacttt caacatcaat aaagatacat taacagttaa ggaagcagat ggcgaagtag
    37921 ttagtcaaac tgttcaaaaa gataaacctg atcctgttga aaatacggag caagagaaca
    37981 aaaaatccga gactacaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa
    38041 caagtgcttc aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga
    38101 aagaagcatc acagggaaca agtgaaagta gcactacgga agctgatact ggaacatcaa
    38161 caaataactc acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg
    38221 atcaatcaca aacaggttca agtgatgaaa ctgacagttc tacttcacca agtaaccccg
    38281 aacaaccaac taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt
    38341 gcgaaggaac atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt
    38401 taatgacttt tgttaaatca atgccactat ctcctgataa taatccaaat aatggttttt
    38461 ctggtgaatg gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag
    38521 tttcatgggg tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag
    38581 gattaaaaga tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg
    38641 aacctgctca acataaatca atgttttggg gcgatgctat ttggaatcga catgtagtag
    38701 ccgttgatgg atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt
    38761 ggattaatgc aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac
    38821 gttaataaaa gagatcgttt atatactaag tagttaagta tagtctttat ttgtccttgc
    38881 ttaactactt agaaaacttg ttgctcatgg ttggtagaac taaaaggggt gatgctatga
    38941 gtactagagc gttgaaacag cttgctttca acgaaaaaag tgattaatag gtgtggtgat
    39001 tttaacttag gaatgggcgc gaaggtcatt accacaccga catatcgtgg ctcattttct
    39061 tcgcctaact ggtataaaga aggagtaacg agagatgaag catgaaaaaa cgataatttt
    39121 aaaatcaaaa ataatttatt gttttgcgac tttagtaatg gtgttatcta cgcttataat
    39181 gcctttttca gcaataagag ctgaagcaga tgtcacaaaa ccgacaagtg caaaatttac
    39241 agaaactggc gtaaaaacag acggttatat tagggttaat atgccgaatc atccaaatga
    39301 atggatgatt tcaagtcaat ttaaagattc tcatggaaat attggatatt gtatggattc
    39361 agaattgcca tccccaacag gaagtggagc cggaagcttg aagtacaagg gagctggcag
    39421 tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca aaaacagcaa aggaactggg
    39481 tgcaggaaat gatacagaag catggtatgc aacacaatta gtttcatggg ttttagccgg
    39541 aaactttaaa gtttcacaga ttgtatggtc acatccaaac cataccgccg cagaaacagc
    39601 tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct aagaatggaa aagatacgcc
    39661 caacactgag ttttctatca cagcttctaa gacagctgac gaaggaaaat atcacacttt
    39721 cacatataaa acagcatcaa ataagactgg taatgcgaag ctcactttta catcagcaaa
    39781 accagctggt atgaagattt atgatgcaga tggtaaagaa attactaaca acactgtgaa
    39841 attaaattca agttttacta ttaaagtacc tgtgactaca ccaagtggta ctttgtcatt
    39901 taaaggtact gctaatgtta gcacaaccaa tccttttact tttgatggac gaggtgtgta
    39961 tcaagatgcc gtagttatga tcactacttc agaaacaaag gactctaaat ctttgagtgc
    40021 taaatggaca agagctgttg gtcaggtaac agttaagaaa gttgatcaag atggaaatgc
    40081 tttaaaagga gcggagttta ctctaactga caataacaat gctaaatctg ttaagacaac
    40141 cggagatgat gggttagcta cgtttgattt gcaacaaggc ttaacataca gcttagaaga
    40201 aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag gatggtatta ctgttaatac
    40261 cgatggacaa gaatttagtt acactgctac gaacacaatt aaaaaaggta aagtaaaagt
    40321 taaaaaggtt gaccaagatg gaaaaccatt agctaatgct gaatttacct tgacggatga
    40381 ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga cttgcaagct tcgatattgt
    40441 agcaaacaag acctacagcc ttgaagagac cggagtacca actggttata caggcgtatt
    40501 caagcaagaa ggaattaccg ttaaagatga tggacaaact tttgaatata cagctacaaa
    40561 cactttgaaa aaaggtttag taaaagttca taaagtcgat caagatggaa aacctttggc
    40621 aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca caagtagaaa aaacagatga
    40681 aaatggactt gcaagcttca atattgtagc aaacaagacg tatagcctag aagaaactgg
    40741 agtaccagta ggttacacag gtgcatttaa gcaagagggt atcaccgtta aagacgatgg
    40801 gcaaactttt gaatatacag ctacaaataa tttgaaaaaa ggtgtagtaa aagttcataa
    40861 agtcgatcaa gatggaaaac ctttagcaga tgcagaattc accttaacgg atgaaaaagg
    40921 tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct acgttcaaca ttattgcgaa
    40981 caaaacttac aacttagaag aaacaaaagt accaacaggt tacacaggcg cattcaaaca
    41041 agaaggaatt accgtcaaag atgatggaca agtctttgaa tatactgcta aaaacacttt
    41101 gaaaaaaggt gtgatcaaag ttcataaagt ggatgatcaa gggaaagcat tagcaaacgc
    41161 agaattcact ttgacagacg acaaaggtgt aaaagttgta caaaaaacgg acagcaatgg
    41221 cttggcaaca tttaatattg ttgccaacaa aacgtacaaa atcgaagaaa cacaaaaccc
    41281 taatggttat acagggaaat ggtctaaaga aggcatcacc gttgctgacg atgaacaaac
    41341 ctttgaatat acagtaatta atcaccttgt acctgaaaca gtaagaacag gcggaatcgg
    41401 tacatcagcc ttccttcttg tatttctaat tgcaggattg agtatggtgc tactgtatta
    41461 tcgagaacag cacaaagcaa aataaaaaag tgttaatgga aaagaagtaa atatgaaaaa
    41521 agaaggctga cgtatgaaat caaaaaaaat tttttcaaat ttaatgctat ttggtacttt
    41581 gctaggagtg ggattgggcg caagctcaat ctcttctagc cttggactag gggggcaagt
    41641 agcatatgca caacaaaact ataattatca aggaactgga acgattcata tccataaagt
    41701 aacaaatact aatggggatg ggaaccctgt ctcagatatt gataacaaag ggaaagaaat
    41761 agctttacca aatggtgtaa aacctcttga aggcgttgag ttctcttatg tgaaacttac
    41821 taaagatgaa gctaaatcct tgggatggac ggatagtgat actacaaaag gtcaacatat
    41881 tgatgatgct aaagcacgat tattactaaa caaaaaatac agtgaagtaa gtagaatgaa
    41941 gactgattca caagggaata tagacatacc tatacaagta aatacagacg atttaacaga
    42001 taacttgtat ttgattgttg aaacaggaac agtcaaaggt gtagttgaag aagcgtatcc
    42061 tatgattgtt aacgtacctg aactaaacgg atcagatgat caatacttta attatgatgt
    42121 acatgtctat cctaaaaact atttccaatt aaactcagca gagcttgata aaaaagacga
    42181 agatggaaag gctcttgccg gaactgtttt tgaattgcga aaagatgatg gatctgatca
    42241 ggttgttgga acttataaaa cagacagcac aggtaaatta actgtagatg gtttggaatt
    42301 aggtgactat tatttcctag aaaccgactt gggaacagat gctaataaaa caacctattt
    42361 actagatggt aaaaaattca aatttagcgt taaacgtggg gagactgcta aagtagttct
    42421 tgatgttacc aactacaaac gacctgtaat tactaaggta attgataaag aaaccgttga
    42481 tactggcgaa gatgttcaat atacattgaa accatcaatt cctggtaata ttggtaatta
    42541 cgaaaaatat gttgtcaaag atgttctagg tgcagagtta acttatgatt catcagaagt
    42601 gatcgaagga atgactttag atactgatta cagtatcgaa cgtgacggac aaacgacaaa
    42661 ctatgtcttt actgaatcag gtatggataa gcttcagaaa caatttgatg caggggatcg
    42721 cttctttaca attttgatga acgctcatac taatagtaaa gctaaaatgg atacaccact
    42781 taagaatgat gtttcaacga tctacaacaa tggatatgaa cccgaagacg aaacaccagt
    42841 tgatcacgct tatacaatca ttggtgatca ccaattcatt aaaacagatg gagatacaag
    42901 caaagcattg agtggagcaa aatttgtagt gaagaacaaa attgatccga ataaagttac
    42961 ttacctaaaa caagatccaa caacgaaagc tgttacttgg attgcagata aatctaaagc
    43021 tactgaattc gtttccggat cagacggtaa gttccaaatt gaaggcttgc aatataccta
    43081 tgatgtagat tttgataaac aaggtaatcc aacaatcaca gataaaaaaa tcaatgatta
    43141 tgctcttgaa gaagttgttg ctccttcagg atatacacca actagaaatg atgttccatt
    43201 tgtggtatcg aatgatgatg cttctagcga agtaacagac atcaagaacc ttaaaacacc
    43261 tgaaacagtt cgtacaggtg gaattggaac agtagtattt ctagtggttg caggtgtggc
    43321 aggaacaggg ctatatgttg tctacaaacg agaacaacac aaagcttaat gaaataaaat
    43381 tccctagagt gtttttggct ctagggtttc tttttaaaaa gtgtgtgaaa agtaggtatg
    43441 agcatgaaaa agaagaatat caagttgatc atcttaaaaa ttgggatgtt gctatgttta
    43501 ggagtaggtc tatatcctgt aattactcag gcttatacct actaccaaac tcaacaaaca
    43561 ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa aggagaatga agaagtcttc
    43621 aagagatatt cagaagttaa taggaaacga aaagacacag aacaaacctc agtcacgatt
    43681 cctgaagata ataacaatat tccgtcaaat gtaaatcttc agaaggataa attaggtgaa
    43741 ttagtgggtg aaattcttat accaaagatt aatgtggatt tgccaattta tgatggtgta
    43801 aacgattttc aactatcagc aggagcagga atattaaccg gaactgatta tccaactggt
    43861 gggattggaa accattctgt tatatcagct cattcgggca ttcctaataa aaaatatttt
    43921 acagatttag agttgctcaa acttggtgat aaatttttta ttgatatttc agggaaaaag
    43981 cttgcttatc aagtagatcg gataaaaaca atcctaccaa ccgaatttga ggatttgctt
    44041 cctgtggcag gaaaagatta tgtaacactt atgacatgta cacctgttgg agataattct
    44101 cataggttac tcgttagagg ggttagcata ccttaccaaa aaagtattgt tgagaaagaa
    44161 aagaagaaag aagatttaaa ggtaaaggcg cagatgaact ggtatcctat cgtagttgta
    44221 ggggttttat taataatagc tgtgatagtc attattaaaa aaatagtagc aaagagacga
    44281 ggaatgaaaa atgaagaata aaatactact ttttttgact cttatttgca ctttggtttt
    44341 aacaatttat cttgcaaatc agctctatca tcaattcaaa acaaaagaga atgtggcgga
    44401 aatccaagag aaaattgaga aaaaacaaac tccttcaaaa gaggaagtgg caaaaaaagt
    44461 cgaacaaata aaatcgaaca gcggtaacca aggattagac aatactcaga aaacagacgg
    44521 cggatcattt gatattggtg aagctgaagg aaaggtcaat ccttacacag gagatgtttt
    44581 ttcaaaaaat caaacgaaag attttgaacc atctacctat tcaggggcgg ttggttatgt
    44641 ctatattcca aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggaacattt
    44701 atatagaggt gtggcacaga tgagtgctac tagtctacct ttatcaggta aaggtcatca
    44761 aacagtaata gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca
    44821 attagtagcc ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt
    44881 tttccgaaca gacgttatta gaaattacgg tcagaattca acactgactg atattcctga
    44941 tttagatatg ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt
    45001 agttagaagt ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa
    45061 taccgcatac gctgatgcca ttgatagcga gtccgtttca gataagacag aaactgttac
    45121 atcttctaat gatttatttg aggatgttga tcgtgccaac aaggaattag atggaaataa
    45181 tgatcttaat attccggaac aaggtagcgt agagatgcaa gacagaacat ttgctgaaaa
    45241 ggcaagttca atattcacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg
    45301 gactgtaata ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttcg
    45361 aaaagcataa tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt
    45421 attgatctct tttttatttt gaatagagct agattcactt ttaaataaaa tcaaaactga
    45481 ttttaattct aaagaaaaag tcaagaaaaa aatacaacaa atttttttat cagaatattt
    45541 atcagaatat tattagaaaa gataaactca aaagcttaat ttaatagggt tattggttgt
    45601 atttagaaat aatgtatgga aaagctataa agaaatagat aagatgtaga aaatcttatt
    45661 tgtgaatgaa aaacaagtca tcgtaaaata ttaataggag gtgtagtaat ggtgggaata
    45721 tcaaaaacag ctttagactc attagctaaa ctatttagta gcaggtttga tagtgcagaa
    45781 tttgttgaat atctaagcca aaacgaaagt ggaaaaactc tgatcgaaac aatgaaaaaa
    45841 gactatttga atgaaaatga ctttccgaca tggacgaata ttgaagatgc tacatgggat
    45901 gtagtgtgta cgatatattc tccttgggta acgattgctg attttgtttt agataaaatt
    45961 ggaggggata cataccctga tgccaaatct cttgctacaa gtgactttaa aacgttctat
    46021 ggttatctaa taggtctaat aggcaatgga atagatttga aagttacgcc aaaacaaaca
    46081 cctgaagaaa ttgaaaaaga aaaaggagag gttcaaatgg cttatgcaaa tgacagagaa
    46141 aaagtaaaat tattggaagg taaattatct gaagaagaac ttggaaaaat ggtgcaagaa
    46201 atcaatgaag acagcaacta ttttcctgaa ttgaaggtat atcaaaatga cgaagatttc
    46261 tttaatgaaa tgtttagtaa tagtccatgg gaagcagtga gtaaaacaaa tttaggagaa
    46321 tggaactttc aagatgatct agttgcattt agcaatgatg ggtatttacg atcatattca
    46381 gaaagtgagt atagagctga attaaaaacg tatgaaacgg atattctgat gagctattta
    46441 aatctattag agagtggtca gggagagtta agagataaag ttgaagaact attaaaagaa
    46501 gagctatagg gattatttca acataaacac aagcagaaaa ctaccaaaca aaacgtacca
    46561 tgcaaaaagt cggttaaacc ggctttttgc tctattatga gaggagatca aaccatgagt
    46621 gcaacaaccg atatggcttt agaagcagta ataaaagata atacaggaaa tgtcgaaaat
    46681 gtagaagact ttagcgatat tgcaatcaat ctttcagcat ggatttcagc tgtaggagaa
    46741 gaaaatatcg aatttgagat caagaatatg gatgacttaa aagcaaaatt tccttttatc
    46801 gcttcgaaac taagtcaatt tgccgaggat aaatcgtgga atgaattagt tagtttttcc
    46861 gaaaaaattt atgatattac gcaagaagat gagtgtgttt catgggaaat gaataatcaa
    46921 tacaagaaaa ttgatgcttt cgtcaaatca cgtatagaag gaacagcttt tgagtttgaa
    46981 ggtatagata ccgcagataa ttggttaaat aaagaatcct ttattaaaag tatagcggat
    47041 gatcttgaaa aacaatggtc taaggaagac tatgcagagt acctaaaaac agaaagagat
    47101 acagaggtga aagaaaaact cactgaggta tgtttggatg aacatattga tcccaatatc
    47161 gtaaaacaag aagactatga agtggttatt gataaagttc ctactgattt tgaggaagtt
    47221 gtaaagtata tagttgaaga agcattatcg caatactctt cagatgagga agtggacgaa
    47281 gaaactgttc gtaccagagt ttggcaactg gtttttgatg acaaagatta taaatatttg
    47341 agtgatcaaa actcggttaa ttggagtgta cgattattaa ttgatcaaaa tgatattgaa
    47401 taaaatagag ctgaaaagct ctattttttt tgataaaaaa actagctatt tttttaaaaa
    47461 taatttggtt caaaatttat ttttttaact tgataatttt ttgccatcct t
//
LOCUS       DACIRY010000005        46936 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.005, whole genome shotgun sequence.
ACCESSION   DACIRY010000005 DACIRY010000000
VERSION     DACIRY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.005"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            130..2727
                     /gene="adhE"
                     /locus_tag="IYE22_000213"
                     /gene_synonym="adhC"
     CDS             130..2727
                     /gene="adhE"
                     /locus_tag="IYE22_000213"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ9505459.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2956..4062
                     /locus_tag="IYE22_000214"
     CDS             2956..4062
                     /locus_tag="IYE22_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ9505460.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4079..5107
                     /locus_tag="IYE22_000215"
     CDS             4079..5107
                     /locus_tag="IYE22_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ9505461.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5113..5592
                     /locus_tag="IYE22_000216"
     CDS             5113..5592
                     /locus_tag="IYE22_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ9505462.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5608..6903
                     /locus_tag="IYE22_000217"
     CDS             5608..6903
                     /locus_tag="IYE22_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ9505463.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6914..7738
                     /locus_tag="IYE22_000218"
     CDS             6914..7738
                     /locus_tag="IYE22_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ9505464.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7903..8802
                     /locus_tag="IYE22_000219"
     CDS             7903..8802
                     /locus_tag="IYE22_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9505465.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8928..9797)
                     /locus_tag="IYE22_000220"
     CDS             complement(8928..9797)
                     /locus_tag="IYE22_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9505466.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9950..11410
                     /gene="rhaB"
                     /locus_tag="IYE22_000221"
     CDS             9950..11410
                     /gene="rhaB"
                     /locus_tag="IYE22_000221"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ9505467.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11407..12663
                     /gene="rhaA"
                     /locus_tag="IYE22_000222"
     CDS             11407..12663
                     /gene="rhaA"
                     /locus_tag="IYE22_000222"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ9505468.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12677..13522
                     /gene="rhaD"
                     /locus_tag="IYE22_000223"
     CDS             12677..13522
                     /gene="rhaD"
                     /locus_tag="IYE22_000223"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ9505469.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13524..13835
                     /gene="rhaM"
                     /locus_tag="IYE22_000224"
     CDS             13524..13835
                     /gene="rhaM"
                     /locus_tag="IYE22_000224"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ9505470.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13917..15068
                     /gene="fucO"
                     /locus_tag="IYE22_000225"
     CDS             13917..15068
                     /gene="fucO"
                     /locus_tag="IYE22_000225"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ9505471.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15195..15953
                     /locus_tag="IYE22_000226"
     CDS             15195..15953
                     /locus_tag="IYE22_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ9505472.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16053..16697
                     /locus_tag="IYE22_000227"
     CDS             16053..16697
                     /locus_tag="IYE22_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ9505473.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16704..17711
                     /locus_tag="IYE22_000228"
     CDS             16704..17711
                     /locus_tag="IYE22_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ9505474.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17727..18569
                     /gene="kduI"
                     /locus_tag="IYE22_000229"
     CDS             17727..18569
                     /gene="kduI"
                     /locus_tag="IYE22_000229"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ9505475.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18589..19392
                     /locus_tag="IYE22_000230"
     CDS             18589..19392
                     /locus_tag="IYE22_000230"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ9505476.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19641..20705)
                     /locus_tag="IYE22_000231"
     CDS             complement(19641..20705)
                     /locus_tag="IYE22_000231"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ9505477.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20702..21787)
                     /locus_tag="IYE22_000232"
     CDS             complement(20702..21787)
                     /locus_tag="IYE22_000232"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ9505478.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21800..22777)
                     /gene="mvaD"
                     /locus_tag="IYE22_000233"
     CDS             complement(21800..22777)
                     /gene="mvaD"
                     /locus_tag="IYE22_000233"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ9505479.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22770..23714)
                     /gene="mvk"
                     /locus_tag="IYE22_000234"
     CDS             complement(22770..23714)
                     /gene="mvk"
                     /locus_tag="IYE22_000234"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ9505480.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24036..24689
                     /locus_tag="IYE22_000235"
     CDS             24036..24689
                     /locus_tag="IYE22_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ9505481.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24774..25679
                     /locus_tag="IYE22_000236"
     CDS             24774..25679
                     /locus_tag="IYE22_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ9505482.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25681..26313)
                     /locus_tag="IYE22_000237"
     CDS             complement(25681..26313)
                     /locus_tag="IYE22_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ9505483.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26633..27157
                     /gene="infC"
                     /locus_tag="IYE22_000238"
     CDS             26633..27157
                     /gene="infC"
                     /locus_tag="IYE22_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ9505484.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27229..27429
                     /gene="rpmI"
                     /locus_tag="IYE22_000239"
     CDS             27229..27429
                     /gene="rpmI"
                     /locus_tag="IYE22_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ9505485.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27482..27841
                     /gene="rplT"
                     /locus_tag="IYE22_000240"
     CDS             27482..27841
                     /gene="rplT"
                     /locus_tag="IYE22_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ9505486.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28093..29430
                     /locus_tag="IYE22_000241"
     CDS             28093..29430
                     /locus_tag="IYE22_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ9505487.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFCGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29478..31607
                     /locus_tag="IYE22_000242"
     CDS             29478..31607
                     /locus_tag="IYE22_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ9505488.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31582..32271
                     /locus_tag="IYE22_000243"
     CDS             31582..32271
                     /locus_tag="IYE22_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505489.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32679..33365)
                     /locus_tag="IYE22_000244"
     CDS             complement(32679..33365)
                     /locus_tag="IYE22_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9505490.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(33501..33695)
                     /locus_tag="IYE22_000245"
     CDS             complement(33501..33695)
                     /locus_tag="IYE22_000245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505491.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33745..34185)
                     /locus_tag="IYE22_000246"
     CDS             complement(33745..34185)
                     /locus_tag="IYE22_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ9505492.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(34189..35034)
                     /locus_tag="IYE22_000247"
     CDS             complement(34189..35034)
                     /locus_tag="IYE22_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ9505493.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35183..36601
                     /gene="pepV"
                     /locus_tag="IYE22_000248"
     CDS             35183..36601
                     /gene="pepV"
                     /locus_tag="IYE22_000248"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ9505494.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36677..37879)
                     /locus_tag="IYE22_000249"
     CDS             complement(36677..37879)
                     /locus_tag="IYE22_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505495.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(37866..39653)
                     /locus_tag="IYE22_000250"
     CDS             complement(37866..39653)
                     /locus_tag="IYE22_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505496.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(39666..40619)
                     /locus_tag="IYE22_000251"
     CDS             complement(39666..40619)
                     /locus_tag="IYE22_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9505497.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(40804..41154)
                     /locus_tag="IYE22_000252"
     CDS             complement(40804..41154)
                     /locus_tag="IYE22_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9505498.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            41354..42433
                     /gene="pepA"
                     /locus_tag="IYE22_000253"
     CDS             41354..42433
                     /gene="pepA"
                     /locus_tag="IYE22_000253"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ9505499.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            42576..42896
                     /locus_tag="IYE22_000254"
     CDS             42576..42896
                     /locus_tag="IYE22_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ9505500.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            42918..43382
                     /locus_tag="IYE22_000255"
     CDS             42918..43382
                     /locus_tag="IYE22_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ9505501.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            43578..44183
                     /locus_tag="IYE22_000256"
     CDS             43578..44183
                     /locus_tag="IYE22_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ9505502.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(44222..45511)
                     /locus_tag="IYE22_000257"
     CDS             complement(44222..45511)
                     /locus_tag="IYE22_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ9505503.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            45939..46418
                     /gene="rlmH"
                     /locus_tag="IYE22_000258"
     CDS             45939..46418
                     /gene="rlmH"
                     /locus_tag="IYE22_000258"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ9505504.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            46621..46899
                     /locus_tag="IYE22_000259"
                     /pseudo
     CDS             46621..46899
                     /locus_tag="IYE22_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT        15041 a         8509 c         9913 g        13473 t
ORIGIN      
        1 aaagcaattt tatatttaaa aataactgtg aaaaaaatca caaaaaagtg tttacataga
       61 aaaataaata ttgtatgata tacatgtgaa ttatttaaca aataaccatg acccattagg
      121 aggataatga tggctaaagt aatagaaaaa gaaaaaacga agacaattga cgtacaagca
      181 atgattgatg atttggcaga aaaagcaaat gcagcattga aagaaatgga aagctttgat
      241 caagaaaaag tagaccatat cgtgcatgaa atggcaatgg cagctttgga tcaacatatg
      301 ccattagcga agatggctgt agaggaaaca ggccgcggtg tttacgaaga taaagcaatc
      361 aaaaatatgt atgcgtccga atatatttgg cataatatca aacatgataa aacagttggt
      421 gtgatcaatg aagatgtcca aaaagggttg attgaaattg ctgaaccagt cggtgtggtt
      481 tgcggagtaa ctccaacaac gaatccgact tccacaacga ttttcaaatc aatgatcgcg
      541 ttgaaaacaa gaaatccaat cgtttttgct ttccatccaa gtgcccaaaa atcttctgca
      601 gaagcagcac gagtggtacg tgatgctgct atcgctgccg gagctcctga aaattgtatc
      661 caatggatcg aacatccttc gatcgaagcg acttctatgc taatgaacca tccaggtatc
      721 gcgatcgtct tagcgacagg tggagctgga atggtcaagt ctgcttactc tacaggaaaa
      781 ccagctttag gtgtaggtcc aggtaacgta ccagcttata ttgaaaaaac agcaaaaatt
      841 aaacgtgcag tgaacgattt gatcgtttcg aaaacatttg ataatggaat gatttgtgct
      901 tctgaacaag ctgtgatcgt ggataaagaa atctatgcag cagtaaaagc agaattccaa
      961 gcacaccaag tttatatcgt aaaaccagat gaattgcaaa aattagaaga tgcagtgatg
     1021 aacgaaggaa aatatgcggt caatccatca atcgttggac attctgcgat ggaaatcgca
     1081 aaattggcag gaatcagtgt accaaaagga acgaaaatgc tgattgctga attggaaggc
     1141 gtaggcccag attatccgct atcaagagaa aaactttctc cagttcttgc catgatcaaa
     1201 gctaacaaca cggaccatgc gtttgatttg tgtgaaggta tgttgaatct tggaggatta
     1261 ggacatacag cagttatcca ttcagaaaac gaagagttgc atgtcaaatt cggtcttcga
     1321 atgaaagctt gtcgtatttt agtgaataca ccatctgcag aaggcggcat cggtgatatc
     1381 tataacgaaa tgattccgtc attgactttg ggatgcggtt cttatggtaa gaactctgtt
     1441 tctcgtaacg tctctgctgt aaacttaatc aatgtcaaaa ctgtagcgaa acggagaaat
     1501 aacatgcaat ggttcaaatt accgccaaaa atcttctttg aaaagaactc tctattgtac
     1561 ttagagaaaa tggaaaacgt tgaacgtgtc atgatcgttt gtgaccctgg tatggtccaa
     1621 ttcggttact gcgatacagt tcgtgaagta ttggcacgac gtaaaaatga tgtgaagatc
     1681 gaagtcttct cagatgttga accgaaccca tcgacaaata ccgtatacgc aggaacgaaa
     1741 ctgatggcag acttcaagcc agatacagta atcgctcttg gaggcggttc tgcaatggac
     1801 gcagctaagg gaatgtggat gttctatgaa cacccagaca catctttctt tggtgcaaaa
     1861 caaaaattct tggatatccg taaacggaca tataagatcg acaaaccaga aaaaacacaa
     1921 ttcgtatgta tcccgacaac atcagggact ggttctgaag taacgccatt tgcggtcatc
     1981 actgacagtg aaacacatgt gaaatatccg ttagcagatt atgcattgac gccagatgtc
     2041 gcaatcgtcg atcctcaatt cgtcatgtca gttccagcat ctgtcacagc agataccggg
     2101 atggatgtgt taacacatgc aatcgaatcc tatgtttcag tcatggcttc agattataca
     2161 cgtggattga gcctacaagc aatcaagtta gtctttgact atttagaaaa atcagtaaaa
     2221 acacctgata tggaatcacg tgaaaaaatg cacaacgctt ctacaatggc tggtatggcg
     2281 tttgctaacg cattcctagg tatttgtcac tcagtcgcac ataaaatcgg cggggaatac
     2341 gggatccctc atggacggac aaatgcgatt cttttaccgc atattatccg ctataatgca
     2401 aaagatccac aaaaacatgc gatgttccct aaatatgact atttccgtgc agatacagat
     2461 tacgcagata tcgcaaaatt cttaggtctg aaaggcgaaa caacagagga attagtcgaa
     2521 gctttagcaa cggctgttta tgaattagga aaatctgtcg gcatcaacat gagtctaaaa
     2581 gctcaaggag tcacacaaga aacactagat acaaccgtag accgtatggc tgaattagct
     2641 tacgaagacc aatgtacgac agctaatccg aaagaaccat tgatctctga attgaaacag
     2701 atcatcattg atgcttacaa cggataagca aaagaaagcg tagaaaaaag ataagaaaat
     2761 agatgaaaaa gaacttggaa aacgttgttt ctccaagttc tttttttaat ttccaagtaa
     2821 ctgaaagtta cttttgttat ttaacattta ggtcaatttt agacagtttt tgttcaaaaa
     2881 gcgatattta tagtggacgg tattactgct atattaagga tataaaatga aagcgtttaa
     2941 aaaggtggcg taaagatgat tattaaagaa gagatttttg tacctaaaga attagtgaaa
     3001 gaaaaaattg acctgctcgt tgaaaacctg acaacgatca aagacgataa tggtgaattt
     3061 ttgttggact ttgatggact gaaagtagat gacaaaagct ggacagtgtg gaattggcca
     3121 caaggtgtcg gtctatatgg tatttataaa aattaccaga tgacaaaaaa tcctcgcgct
     3181 tatcaagtgg tcaatgaatg gtttgaggat cgtatggaag aaggcgctcc gcccaaaaac
     3241 gtcaatacaa tggcaccgct attgactatg gcttacttgt atgaagacac gaaagattca
     3301 cgctatttgc cttatttaga acaatgggca gaatgggtca tgaatgacat gcctcggaca
     3361 aatgaagacg ggttacagca tgccacatat ggaccggaaa ataagaatca gttgtgggac
     3421 gatacattga tgatgacagt gcttcctttg gcaaaaatcg ggaaactgtt gaatcgccca
     3481 gaatatttag aagaagctcg ttaccagttt atgatccata tcaaatactt gatggataaa
     3541 cgaacaggat tgtggtatca cggctggacg tttgaaggaa atcataacta tgccgaagca
     3601 ttttgggctc gtggaaactg ctggttgacg attgctattc cagagatcat tgaaatctta
     3661 gaattatcga aagaagatgc attgcgcaaa ttattgatcg aaacacttga agcacaagtg
     3721 cgtgcattaa aaacttatca gtctgaaagc ggtttatggc atacgttgtt ggatgatcct
     3781 tcttcttacg tagaatcttc tgcaactgca ggatttgctt acggtatttt gaaagcggtc
     3841 cataaacgtt atttaccaca agaatataaa gaagtcgctt atcgtgctat ccaaggattg
     3901 cttgaacaaa tcgatgaaaa aggagaagtt caaaacgttt caatcgggac aggaatgggg
     3961 gacagtttag atttttatcg gaatatcgga atcacagcaa tgccttatgg acagtcgtta
     4021 acggtgcttt gtttgacaga attgctcgtc tcttattgtt aaaagaaagg aaacccatat
     4081 gaaaaaaaca tcactgctgt ttgcagcaag ttcgcttagc ctaggtctgc taggtctaag
     4141 cggctgcgga aataattcta gtgtggcaac tgtcgcagat gcagatgtaa atgagatagt
     4201 taccttgcgc tttgcttatg caagcaacag ccagccagta attgatgcaa tgaatgaatt
     4261 tgggcgttta gtaaaagaaa aaacagatgg ttccgtacag atccagtatt tcccagatgg
     4321 acaactgggt ggagaacgtg aactgatcga attgacacag tcaggagcag tagatttcac
     4381 aaaagtaagt gcctcagcgt tggaaagttt ctctaaagat tattcgattt tttcagttcc
     4441 ttatctattc aatgatgaag aacatttctt taaggtgatg gataatgaag aaataatgga
     4501 gccaatctac caatcgacca aaaatttagg atttaccgga ttgacctatt atgattctgg
     4561 tcagcgcagc ttttatatgg tagacggtcc gatccatacg ccggaagatt tggagggaaa
     4621 gaagatccgt gtgatgcaaa gcgaaaccgc tatcaaaatg gtagagcttt taggcggttc
     4681 gcccgtacct atgggaagtg atgaagtgta cacgtctctt caatccaatt tgatcaacgg
     4741 atcagaaaac aatgaatttg tcttgtatac agctggacat ggtggtgtgg ctaaatacta
     4801 ttcctatgat gagcatacac gtgtgccaga tatcatcatc atgaatgatg ccatcaaaga
     4861 acagctaact gatgagcagg aaaaagccat tgaagaagca gcgaaagaat caacggtttt
     4921 tgaaattgaa gcttttgcaa aagcaattga agaagaaaaa gctttagcac aagaagaaca
     4981 tggagtccaa ttcaatgatg tagataatac accgtttcga gaggcagttg ccccacttca
     5041 tgatatattc aaaaacgatc ctacctacaa agacctttat caaaagatcc aagagcaagg
     5101 agcgtagctg tgatgaaaat gattttaaac aaggtattag agatcctggg agctgttttg
     5161 cttgttggga tggtcgctgt tgttttatgg caagtatttt ccagaacggt tttaggaaat
     5221 ccaaatacgg tcacggaaga gcttgttcga ttcggtcttg tctggtttgc catgttgtct
     5281 tccgcgtatg ttgtgggaca aaaagcacat ttagccgtaa cggttttaag tgaaaaatta
     5341 actggcaaaa aaagtaaaat tttagaagtt gttgttcaaa gtttatttct ggtatttgct
     5401 gcgatgatca tggtgtatgg cggatggaat gctgtcacat tgacaatggg gcagatctcg
     5461 ccttctttaa gtattcctat gggatatgtt tatctgtcag tacctgtatc gggggtactc
     5521 atcattatct atagcctgat caatctaatc gatacattaa gcaacaagcc agcaattcaa
     5581 gaaaaaatat aaaaatggag gaataaaatg gcaatagaag ccggattagt gttatttttt
     5641 gtatttgttg tgttgcttgt attagggatg ccgattgcta taagtattgc attctcatcg
     5701 atgatgactt tattgttagt tataccgttt gatgtttctg cttttagttc agctcaaaaa
     5761 atggtgggaa gtatcaatag tttttcgctg atcgctattc cgtttttcgt tctctcgggc
     5821 attatcatga ataatggagg gatagctaag aaactagtgg attttgcgat gttgttcgtc
     5881 gggcgcgtgc cgggggcttt ggctcatacg aatgtgttag ggaatgcttt atttggttcc
     5941 atctctagct cggccattgc cgcttctacc gcgattggcg gagtgttgat ccctcagcaa
     6001 gtggaagaag gatatgaccg gaagtttgcg acagcggtca atatcgcgtc agctccgaca
     6061 ggtatggtca ttcctccaag tactgctttt atcatgtatt cacttgttgc tggtggtgct
     6121 tcgatctctg ctttgtttat gggaggttat ctcgtaggcg ctttgtgggc gctaggaatc
     6181 atgctagtag cattcatcgt tgctaaaaag aataaatacc ctgttgtcga aaaaggacag
     6241 atggctcatg ctggaaaaat aataaaagag gcgattccta gccttctatt gattgtcatc
     6301 attatcggtg gtatcttgac ggggatattt acagcagtag aagcttcagc gatagctgtc
     6361 atttactccc tgttgattgc tatgttttac tataaaacgg taaaactaaa agatatacct
     6421 aatatgttgt ttcaatcagt agcaatgtca gggacgatca tgttcttatt ggctacttct
     6481 tccatgatgt ctttcgccat ggcgtttacc ggtattcctc aggcaattag ttcgttgatt
     6541 atgggcgtaa cggataataa atttattatt ctgttattag tcaatcttgt gttattactg
     6601 gtgggtatgt ttatggatgt ggcgcctgct atcttgattt ttacaccaat atttttacca
     6661 atcgtcacaa gtgtaggggt tgatcctgta cattacgggt tgttttcaat catgaatctg
     6721 tgtgtaggct ccatcacgcc gccagtaggt acaggactat atgtcggtgc aagtgtagga
     6781 ggcgtaaaag cagaggcaat gctgaaaccg cttcttcctt tttatggggt tatcttagtc
     6841 gtcttgtttt tgattactta cttcccgcaa atcgtcatgt ggctacccaa tatgctgagt
     6901 taggagaata gttatgttga acattacaat tagaggacat gatctttccg aagtccaaac
     6961 gatagaagat cttgcagaaa aaacaaaaga acaagggatt catacactgc agctcgcctt
     7021 aggtctttcg tttcctgaaa caccttctgg cgcaaatgag atcaattctg ggatgggaaa
     7081 ttatgtcaaa cgagttttag aaaagcaaga agtgaccgta ggaattttaa gttgctatat
     7141 caatatgatt catccagatt taacgatacg acaagagttg ctgacaaagt tcgagcagta
     7201 tcttcgctat gcttcttcct ttggtgcgtc gatggttgct tcggaaactg gatgtgtttt
     7261 gccggaaatc caatatacgg aagaaaattt cacagatgaa gcatttgctg aagctgtgtc
     7321 cgttatccgt cgcttggtaa aagctggaga aaaataccaa atgatggtgg ggatagaacc
     7381 aggtttgaat catccggtgt attctttagc aagggtagag cagctgatcc aagcagtaga
     7441 ttcggactat ctaggaatca ttttagatcc gaccaatttg atcacaagta gtgattatca
     7501 agaacaagtc caattggtag aagaagcttt tgaacgtttt ggtgaaaaga tatgtgcggt
     7561 tcatttgaaa gattttcgtg tcgaacagga aaaaatcgtg ccggtaaatt tgggagacgg
     7621 tgtgatcgag tacacaaaaa tcaaagaaat catcatgaag aatcgtccgt acctgtatgt
     7681 ggttcttgaa gaaacaaaag atgatgggat tcgttatgga agaagtcttc ttgaataatg
     7741 taaagaagac gttggttaac taatatttag aatggagggg gcggggctag aagatgccac
     7801 gtctcttctt ttttataaga gtatataaaa gcagctcttt ttgattacaa aaaacgtggt
     7861 ataataaaat tgtgcataag tgaacaaaag gaggtgcggc acatgctcca aaagaaaatc
     7921 agcggtgaac aagtcaatac gatggtttct gatcagtatg aaatctatca tgtcaaagat
     7981 gtcgtcagcc aaaataaaac gatctatcat catcatgatt tctatgagat ccatgctaca
     8041 ttaaagggag aagcgacttt ttatttgaac ggacgacaat ttacgatcaa agcaggaagt
     8101 gtgctgctca tccattccaa tgatttgcat cgtatcgtcc gccaatcttc agatgtattc
     8161 gagcgtgtct atatctttgt caccccttct tttctagaaa gtcgttccac gccatggtct
     8221 aatctatcgg cttgttttca accaatggga gagaagagga gtcgtgtatt gcaaatagaa
     8281 caggaagtat tgaagcaaaa attatcattt atcgaccatc ctcctgataa aaaaatttac
     8341 ggtgaagaca taaaatatga acaagctctg gtagaatact tgatttttct caatcaagtt
     8401 gtacaaaaag aagagagtgc gcaagaacag aatgctgtag ttcctaatga gcgtgtggaa
     8461 caaatgttac aatatatctc aaaaaattta tctcatccgc tttctttaga ggaaatggaa
     8521 aagcagtttt ttgtcagcaa ataccatatc acacgagaat ttaaaaagta cactggattt
     8581 acgtttcatc agtatgtaat gaagaaaaag ctgatctatg ccaagcactt gttgcgagaa
     8641 taccgcagct ctagtgctgt atatagtaaa agtggttttt cttcttatcc gcattttctg
     8701 aaagcattca agagagagtt cggcataacg cctaaagaat ttttgaaacg tgatacacag
     8761 aaacagttgg ttcattatga acattttgaa gatgaacaat gagaatgatc actaaaaaat
     8821 aaccgcagta gctgctgttg tttacataga agcagaaact gcggttattt tatttttatc
     8881 tatatatttc aaaatatttt gaaaaaatga cgtacatatt atatgaatca gctgaattta
     8941 cgaaagtctc tcggcgatag tcctgttttt gctttgaaca ggcggctaaa gtaagcaaaa
     9001 ttctcaatcc ctatttcttc ggaaatcatt cgaaccgtaa attctgtata ccgaagaagg
     9061 tctttagcgt aagaaagttt tttgctgatc aaataatcga ttggtggaga accgataaat
     9121 ttggaaaaat ctttgctcag ctgatattta ttcaataaga attgttgttc taattgatca
     9181 agtgtaatcg tttctttgaa atgttcgtct aagtattgct tcagtttgta aacgtaggcg
     9241 ggaatatcct cttcttcgaa atctagctga tacttttgca aaatcagttc attcagcaac
     9301 tcgtggatag ctacagagga ctggaaatcg gtacgggcat tcgtgccttg ttgcatgtgg
     9361 atcaattctg tgatcaaatg gtggatcttg ttttctgatg gatcccctgt tgaatggaag
     9421 gtgttatggc cgtcttttgt gaactcctga taaaatgatt ggacgttctg accattcaaa
     9481 tggatccaat ccatttccca tggttcttcg ctgatcgttt gataacgctg atattcttgg
     9541 cagtcaataa aaaataagtc tccagctttt agctgttgct gattatcatt atatgtaagc
     9601 gaacctttgc cagacaaagt aaactttatc aagtaagaag gtaaatgtgc tcgttcagta
     9661 tagtaaggga ttggtgcttt gaagtaaccg atttcttgga tataaaaaaa taaattcctg
     9721 cttctttgtg taggcgtcaa gatcactctt tttgagtctt ccgaccatga gtggttggct
     9781 ttttcccagt aatccatgta aacactccca acttgtcaag ataatgcaat tatataccaa
     9841 aatagtgtga agcaaaatat aagattctgt attatgatag cattgttaat aaatgataac
     9901 gctttattaa taaagtgcta atcaacaata aagtaaaaga ggtggagaaa tgaatccaac
     9961 atttgttgcc atcgatatcg gtgcatcaag cggacgacta atgaaaagta cgagggatgc
    10021 aaaaggttct ttgatcttgg aagaagtaca ccgtttcaaa aacggctttc ggaacaaaga
    10081 cggtcacgat cgctggaaga tcgattatct tatcaaagag attttgattg gatttgaaaa
    10141 attaaagcag acagggattg atcgcctttt tgcggggatc gatacatggg cagtcgatta
    10201 ctgtctgatt gatgaacaag gggatcggat aaatgatccg attgcttatc gggacaagcg
    10261 taccaatgga gcagtagaac agttttctga aatctattct ctagataagt tgtatgagca
    10321 gacagggatt cagatccagc ctttcaatac cttgttccaa ttgtttgctg aagaaaaaga
    10381 aaatttggca aaagcacata aattgttact gatacctgat tatttagggt atgtctttac
    10441 tggtaacatg gtgacggaga aaactaatgc atctactatg cagctgctga atgcagggac
    10501 aaaagaatgg gaaatagaac ttcttgaaaa aatcggtgtc tcgaaggaat tatttccacc
    10561 attgacagag ccgggaacga tactaggaga tctgcaaaaa gaacaatttt caaaatatga
    10621 tttgccagat gtgaccttta tcacagtagc aagtcatgat acggcgtcag ctgtattagg
    10681 aacgccaggt caaggagaac gttggggtta tatcagcagt gggacatggt cgcttttagg
    10741 gattgaaaca aagatcgcca atgtttctcg caatgctttt tctgaaaatt atacgaatga
    10801 atggggtgcc cataacacgg tgcgtttttt gaaaaacatc atggggatgt ggttgatcca
    10861 ggaagttgcc cgaatgcaag agtacgagta cagttatgcg gaattggcag agcttgctgc
    10921 aaaagaaaaa ccgctgcagc aagtgatgga aatcaatgac cctcgatttt tgaatccccc
    10981 aaatatgata gaagagattc agcaatactg ttttgaaaca gagcagccag tccctgtctc
    11041 atcgggagct atcgctcgtt gtgtgtatga taatcttgct ctttgttacg cacaagagct
    11101 gatcaaagta gaacacttaa ctggaacgaa attggaaaag ctgcacatcg tagggggcgg
    11161 atcgaataat cgtctactga atcaattgac agcagattat gccggaatca ttgtagaagc
    11221 aggacctagt gaagccacag caatcgggaa tgttgccatg caaatgctca gtacaggtca
    11281 gttttcgaat atagaacaag caagaaatgt aatcaaacag tcgtttccat gtgaaacttt
    11341 ttacccagat gaaaataagc aaaacacgat ggaatggtac gtgaataaaa tcaaaggagt
    11401 aatcgtatga acacgattga aagaaattac gaacaagcaa aagaaaaata tgcaactatc
    11461 ggtgtagata cagatgctgt actagaaaag atacaggaca tcaaaatttc tatgcattgc
    11521 tggcaaggag atgatgtcaa aggtttcttg acaccggatg gcgaattgac cggtggaatc
    11581 atggcgacag ggaattttcc aggagccgca cgaacacctg aagagcttag acaagatctg
    11641 gagaaggctt attcgttgat accgggcaaa cacaaattga accttcatgc tatctatttg
    11701 gatacagaag aatctgtcga tctaaatgaa attgagccga aacattttga aaaatgggta
    11761 gagtgggcaa aaaaagaaga aatcggtctt gacttcaatc cgactttctt ttcgcatcca
    11821 atgatgaaag acggctttac attggctcat ccagataaag aagttcgtga atactggatc
    11881 gaacacggga aaagaagccg caaaatcgcg gagtactttg ggaaagaaac tgggcaaaca
    11941 agtatcaata acttttgggt acctgatggg atgaaagata atccgattga tcgttttacc
    12001 ccacgggaac gtttaatgga atcactcgat gaaattttta cagaagagtt agatgaacgg
    12061 tatgtacaag atgcagtaga aagcaaacta tttggtttag gcgctgaagc ttatacagtc
    12121 ggttcccatg aattttatat gggttacggg ttgactcgtg acaaacttgt ttgtctagat
    12181 gctggacatt ttcatccaac agaagtgatt tccaataaat tatcttcatt agctttattc
    12241 agcaaaggaa tcatgttgca tgtttcccgt ccagtgcgtt gggattcaga ccatgtggtt
    12301 ttgatggatg atgaattgca ggaaatcgct aaagaattag tccgtaacga attgttggaa
    12361 aaaacaaata tcggtctgga tttctttgat gcaacgatca accgtatcgc tgcttgggtc
    12421 atcggtacga gaaatacaca aaaagcactg ctgaaagcta tgctggaacc agttgagcga
    12481 ttgaaagaaa tggagctggc ttttgacttt acttcaagaa tggcttatac ggaagaattg
    12541 aaagatttcc cgtatgccga tgtatggaat tatttctgtt acaaaaatca agttccagtt
    12601 ggtttggatt ggctggaaga agtccaggaa tatcaaaaag acgtgttgga attaagaaaa
    12661 taggagtgtg gctatattga aaaaaataga tattttacaa gcgccttatg tgaaagaaat
    12721 gatcgaaacg acgtcgaatc tttatcgctt gggatgggat gaaagaaatg gcgggaatat
    12781 ttcttattta ctaaaagaag aagagatcac accgttcttg gatcctgaaa aagtgatccg
    12841 ccgcatctcg atgatttttg atgccagccg tttagcagga tgctatttta ttgtaactgg
    12901 ttcaggaaga tattttaaaa atgtagcgag cgaacctgcc gagaatcttg gaatcgttcg
    12961 agttgctgaa gatggcaaaa cactggaact tttgtggggg ctggaaaacg aatgccttcc
    13021 tacaagcgag ttgccaagtc atttcatgag tcatatcgcc cgtttagaag tagacccaga
    13081 taatcggatc gttatgcatt gccatgctag tcatctatta gcgatgagtt ttacgcatga
    13141 attagacgaa cgttccttta gtcgtacgct atggcaaatg tgtacggaat gcttagttgt
    13201 ttttccagaa ggaatttcca tcattccgtg gatggttccg ggaacaaatg agattgggga
    13261 agcaacagct gaaaaaatga aagaaactcg attggtttta tggccacaac atggaatcta
    13321 cgggtcagga aaagacatgg atgaagtctt tggtttgatc gagacagcag aaaaggcagc
    13381 agaagtctat acgtacgtca aagcgcaagg accaatcttg caaacaatca cggacgaaaa
    13441 cctgtggcat ttagcagatg cgtttggtgt cactccaaaa gcaggttatt tggaggaagt
    13501 tcatacgaaa gcaggtgtct gacatggtga aaaaagcttt ttgcatgaaa gtttacccag
    13561 aaaaacaaga agaatacaaa aaaagacatg atgaactttg gccagaaatg cgtacagcac
    13621 ttcgtgaaca cggggtcatc gactattcga tctatcttga tccgagtaca agtttgttat
    13681 ttggttactt agtgatcgaa gatgaaaagt tatgggaaaa aatggcaaat acatctatca
    13741 atcaaaaatg gtggaagtac atggaagata tcatggagac aaatccagat gcctctccag
    13801 tcagttggga attgatgcca gtctttgaac tgtagcaaca agcaactggg caaccggttg
    13861 cttggggttc tttcgttttt attgtgaaaa ataatacaaa ttaggaggaa tagaaaatga
    13921 gtaatcgaat gattttaaat gaaacatcgt attttggaaa aggagcgatt gagtcgatcg
    13981 tccctgaatt tcagcagcga ggattcggca aagcagctgt cattactgac aaaggattga
    14041 tcgagcacgg tatagcaaca aaagtaacag aacttctaga taaagcagca atcgcttatg
    14101 ctttatacga cgggatcgta cctaatccaa cgatccaaaa tgtaaaagat ggagtggctt
    14161 ttgttaaaga ggcagaggca gactgtttga tcgcaatcgg tggaggttcg ccaatcgata
    14221 cggcaaaagc cattgggatt attttgacga atccagagtt ttcagacgtc attagtttag
    14281 aaggtgttgc tgatacgaaa aatccatgtt tacctatatt agctatacca acgacttctg
    14341 gaacagcagc agaagtaacg atcaactacg tcatcacaga tgaagtaaat caccgaaaat
    14401 ttgtttgtgt tgatcctcat gatatcccaa tcgtcgcgtt catcgacagc gatatgatga
    14461 tgggaatgcc gaaaaaattg gctgcatcaa caggtatgga tgctatgacg catgctattg
    14521 aagggtttat cacaaaaggc gcttgggaaa tgactgatat gctgcattta aaagcaatcg
    14581 aaatcatcgg ccattctttg gaagtttctg tagatggcga tcaaaatggg cgagaaaaaa
    14641 tggcattagg acagtatatt gcgggaatgg gcttctcaaa tgtcggttta ggactggtac
    14701 acgggatggc tcacccgtta agtgcatggt acaacattcc tcatggcgta gcgtgtgcag
    14761 cacttttgcc aactgtcatg aaatataaca aagaatatac tggagaaaag taccgtgaga
    14821 tcgctcttgt tctagggatt aaaggagcag ctgaaatgtc tttagaagat gtaagagaag
    14881 cggcttgtgg tgagatcgat cggttgagca aagcggttgg aatcccagaa accatttctg
    14941 aattaggtgt aaaagaagca gatattccag caattgcaga agatgctttg cgagatgtat
    15001 gcacaccagg aaatccaaga gaaacgacag tagaagaaat cattgcttta tatcaaagtt
    15061 tgatgtaaaa acgaagcgga tcttcaacat taaattgcct gtaaagagaa atggtagttt
    15121 atatctggat atctctttta aattgatggt atttccgcta taatttttct tataaagaaa
    15181 taggagaata ttatatggaa aataaaaagc cttatggaac tgtattgatc aaagcctcta
    15241 aaatactaga ctatttagcg gaaaaccaag atgtctcttt gcagacgatc gcaaagggtg
    15301 tgggaatgac ctcttctaca gtcttgaaaa tactagatac tttgttgatg atcggctacg
    15361 taaataaaaa ttcagagaaa aactatcgtt taggtgcaaa gcttattcgc tacgcgaata
    15421 aaaacattga acaaatcgat ttggtagagt tgactttacc gtttttagaa cagctgcagc
    15481 aaaagatcga tgagactatc cacttagggg tattggacaa caatgagatc ttgtacgtga
    15541 acaaactaga tccaaaacat caaacgatcc gcatgtcttc aaaaatcgga atcacccgac
    15601 cattatacaa ttctgcgatg ggcaaagccg tattagcaga attcagtgaa gagcagtaca
    15661 ctgattatct tgaaaagcat ccgttgatcc cttacacaga atatacgatc accaatgcat
    15721 tacggttgaa aaaggaaatc caaacggtaa aagaaaccca catcgcattt gacgatgaag
    15781 aaatcgaacg tgatattttt tgtataggag cctcaatcgt gaaaaacgaa caaatcattg
    15841 gtgcatttag tgtaagtatg ccgaaatatc gtttaacaga tgagacaaaa gaacaaataa
    15901 tcagtgccct caagcaaaca aaaagcgaaa tcgagcaaag aattcaaaaa taatgaaaaa
    15961 agcgtttgca atttttcttt tctgccgtat aatatgggta tagatttcca agttataaaa
    16021 taaaatttct aaataggaaa taggtggaga agatgaaaaa aattgatatt ttatctcgat
    16081 tgaaaaacgc cggagtgatt gcggtggtac gcggaaaaag caaagaagaa gctttgaatg
    16141 cttgccacgc aatcatcaaa ggtggtctaa ctggtattga gttgactttt acagtccctc
    16201 aagcagatca agtaatcaaa gaacttttgt cattctacaa agaccaacca gaaatagtga
    16261 ttggcgcagg cacagtatta gacgctgtca cagctcgctt ggctatctta gctggcgcag
    16321 agtatattgt tagcccttcc tttgatcaag aaacggcaga gatgtgcaat ctgtatcaaa
    16381 ttccttactt accaggatgt atgactatta ccgagatcaa aacagcgctg aaaagcgggg
    16441 tagatatcgt caaactattt cctggaagcg cttatggacc aagtatcatt tctgccttca
    16501 aggcgccgat gcctcaagtc aatatcatgc caactggcgg tgtgagtctg gacaatatga
    16561 aagagtggtt cgatgctggt gtcgtaacag tcggagtagg tgggaacctt ttagcacccg
    16621 cagctacagg agattttgac aaagtaacgg aagtcgcgca acagtatgca gcaaaaatga
    16681 aagaaatcaa gaggtagctg cttatgggaa aagtagtgac actcggagaa atcatgttgc
    16741 gattgtccac agagcaggga acccgtatcg attcaagtga acgattcgct gcccattatg
    16801 gcggagggga agcaaacgta gcaatttctt tagctaatta cggacatgaa gcgatttttg
    16861 ctacaaaagt acctgataac gcattgggaa aagcagtcaa aaagcacttg caatgttatg
    16921 gagtagatac gaaacaggta ttatctggtg gatcaagact aggtacttat tatatggaaa
    16981 ctggtagtgg cgaacgcgcc gcctcagtga tctatgatcg tgccggatca agttttgctg
    17041 aaatggatca tttggaatgg tcgctagagg agttattcca aggaatagat atatttcatg
    17101 tttcaggaat caccccagct ttgtctaaaa aatggcaaaa aatgacgaaa caggtgatag
    17161 aagctgcaaa aaaagctggc tgcctgatca gcttcgatat caactatcgt ggaaaattat
    17221 ggacacagca agaagccagc gaagtaatcc atcagctttt acctttagta gatatctgct
    17281 cagctggaaa aatggatgct ctttatttgt taggtatcga gaaagcgcca gaagcagaag
    17341 aacaacctct aatttattat tatcaaaaaa tgcaggaaaa gttcccgaat attcaggtgt
    17401 tttattcgac caagcgtacc gttcattcag caagtgaaaa tgaattgaag gggacattgt
    17461 ggatgaacca tgaatatgtg gaatcccaag tccatagaat caccccaatc gtggatcgag
    17521 taggtggcgg agatgctttt gcaggtggcg tgctccatgg gattttatcc aatatgtctc
    17581 cgcaagaaat cattgatttt gctacggcag cttctgcctt gaagcatacg atccatgggg
    17641 actgcaacca atttagtcaa gaagaagtcg cacaattttt agcatgcggc tctggaaaaa
    17701 tcattcgata gaaggagtag aagaaaatgc aagaaatgga aacacgttat acacacagtc
    17761 cagaagatat ccgtcattat tcaacggaac aattgagaaa agaattttta gtagaaaagg
    17821 tatttgaacc agggaaaatc agtttgactt atacgcacaa cgatcgaatg atctttggtg
    17881 gagtgacacc tactgaaaaa gaattagaaa tcacattaga caaagaacta ggtgttgatt
    17941 acttcttaga gcgtcgtgaa ctaggtgtga tcaatatcgg tggaccagga ttcatcgaga
    18001 tcgatgggca aaaagaagaa atgaaaaaac aagacggata ctatatcgga aaagaaacac
    18061 gacatgttgt attttcttct caagatgctg aaaacccagc taaattctat attagttctg
    18121 ttccagctca tcataaatat ccaaatgtaa aaatcagtat cgaccaagta aaaccaatgg
    18181 agactggtga aggcctgaca ctgaatgaac gtaaaatcta ccaatacatc catccaaatg
    18241 tatgcgaaag ctgtcaatta caaatgggtt atacgatttt agaaccagga agcgcttgga
    18301 atacgatgcc ttgtcataca catgaacgtc gtatggaagc ttatgtctac tttgattatg
    18361 ccaatgaaga tacacgtgtg ttccacatga tgggcaaacc agatgaaaca aaacatttag
    18421 ttgtagacaa tgaacaagcc attatttcgc caagctggtc gatccattca ggtgtaggta
    18481 caagcaatta ctcattcatc tgggcaatgt gcggagaaaa tatcacctat acagacatgg
    18541 atatggtgca aatggatcaa ttaaaataag cataagggag agaaaatgat ggaatttagt
    18601 atggatatgt ttcgtttaga tggaaaagta gcattagtca ccggtgctgt ctatggtatt
    18661 ggatttgaga ttgcacgctc tttagctaca gcaggagcaa aaatcgtatt taataacttg
    18721 aatcaagaat cagttgatga aggtatagcg cattataaag aagcaggcat cgatgcaaaa
    18781 ggttatgtct gcgatgtgac cgatgaagaa gcggttcaag cgatggttca acagatcaaa
    18841 gaagatgtag gttcagttga tattctagtc aacaatgcag ggatcatcaa acggacaccg
    18901 atgatcgaaa tggatgcggc agacttccgc caagtaatcg acgtggattt aaatgcccca
    18961 ttcatcgttt caaaagcagt tattccggat atgatcgaaa aaggcggcgg taaaatcatc
    19021 aatatctgct ccatgatgag tgaattagga cgcgaaacgg tcagtgctta tgctgcggca
    19081 aaaggcggat taaaaatgct gacgaaaaac atcgcttctg aatacggtca atacaatatc
    19141 caatgtaatg gaattggacc aggttatatt gctacaccgc aaactgctcc attacgcgaa
    19201 acacaagaaa acggtgaacg tcatccattc gatcaattta tcgttggacg tacgccagca
    19261 gcacgctggg gcgagccaat cgatttggct ggaccatctg tcttcttggc ttctagcgca
    19321 tctgatttcg ttaacggcca tgtcttgtat gtggacggag gcatacttgc ctatatcgga
    19381 aaacaacctt aaaagagctt tagtacactg tttagtggag aggttgtgaa atcagcggat
    19441 tgaacgaata ccgaaggaca gctgattaaa catcatttat tcgaaaaaag aggctgggac
    19501 agaagcattt aactccaaga aataagaagg aattcacgaa aattgctttt caaatttttg
    19561 tgaatttcag cttatttccg aaggagttgc ttttacttcc gccgtttgta cgtgtttaga
    19621 gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttctgtgcg tcttccatac
    19681 ttcactaagt cgatccctct tgcttcgcac caatccttca ctggtccgga aaagatcaga
    19741 gattgttggt gtagggccgc ggtattcgtt gcgccgacca tagtataaag cagacgaagc
    19801 tcttcttgcc attgcttcat cagtataata gtttcttcta cgccgtgatt catcagatgc
    19861 gtcagtacgg ttcctgaagt acccacggca tttgcgccga gacaaagggc tttaaatatg
    19921 tcataggcat tgcggatacc tccggaggct aggatttcca tagaagtgtc tgcttcattt
    19981 gcttcaagca atgaagagac tgtcgattgc ccccagtcag ctaaataact cagttcccgc
    20041 ttggagcgac gggcgttttc gatctgggtg aagcttgttc cgcttcgacc gctgatatca
    20101 actgtatgaa ctcctaaagc agctagatca ttgagcgttt ctcttgtcat gccaaaaccg
    20161 acttctttta cgatgagggg gacgtcaatg gctgtttgag tttcttgaat aagtactttc
    20221 caattggtaa aatcacggtc tccctcaggc ataaccaact cttgaggtgc attcaaatga
    20281 atctgtaagg catctgcgtg aaacaatcgg atagcttctt tggctctttc gacagaagtc
    20341 cctgcaccga tgttggcaat gattttcccg tgaggatatt cttgtcgcat aatcgtataa
    20401 gtatcggcta aagaagggac tttcaaggca gcgctgactg aaccagtggc aatcatcaaa
    20461 tctgcttctc ttgcgacgat tgcaagatct tggttgattt tttttgtttt ttcgcttccg
    20521 ccagtcatcg cattgatata aaatgggctg cttagtgtaa acccaactgc ctgtgtactt
    20581 agatcgacat cagaaactgc tatttgcggc aaagggttat ggatgatccg tacgaagtca
    20641 aattcgtttt tttgtttatc atgaaaggct ttagctagtg aaacatgttc gtcttttcga
    20701 ttcatcggtt ttcctttctt tgatcgctat agacatgtaa cggcagtgga gtgatttctg
    20761 ctttttccca tgcactcatt aaaggaagaa tgccagattt ctggtcaaca attacgattc
    20821 cgcaatcgcc cccacctgca ccagaagatt ttgcggctcc ttcatactgt tcagctaaat
    20881 tacacaattt gttcaaagca ggcgtttcga tgacgacccc agtgattgca gataaatcat
    20941 gcagtaattg tcggtttttt cgaatcatct gttggatcaa cgttacatta ttttctttaa
    21001 accctttgat catttcgttg acacattctg tactattttt taagaaaagc tgataagcca
    21061 ccattttatc ttctctcgaa cggtgaactt gatcgaccaa atcagaagta gaggcagggc
    21121 tacccgtcca accaatcaat aaacgtaaat cttcaggagc aatcaatggc tcaatggata
    21181 gacctggcca atccagggca agtaactcac tgatagaatg ctgagtttct tgttcttgga
    21241 gccaaggatg gtcgaaggtt gagaaagcga tccagccacc ataacagcta gcagcgatgt
    21301 cgccgcagga accattatct tgaactgcta aattggctag tgctgcaatc ttgaaaatct
    21361 ccaactgaga taaattcaac gcgtaaaaaa cattcaaggc tttgaccgtt gcaacggtca
    21421 cggcaccgct agaacccaat ccatatttcc ggccatttga actgtctaac tcacttgtta
    21481 ctttcagatc ataaaaggat aaaagaatgt ttttttcttg tgcatacttt tcagtcaagc
    21541 gaatagcagc aaggatataa tgaaaaggat tttcccgaat atctaaaact aattctccgt
    21601 tgcgtcttgt ccaacgtaca ggcatcccac tatattgtgc agattggata cttccgactt
    21661 ttcgtgcaga ttctacagtg actgtcacga attgatcgac tgcagcgata actgctggat
    21721 ggcctgtttc aacaactgca tattctccgg caatatagag tttgcctggt gcagatactt
    21781 caatcataaa aatctccctt tattcaataa tcgcaattcc tggaccagca tgtgcagtaa
    21841 ttacttgttc tttgctaaat aatttggtaa atgtttcttg tacctcagat aaatggctgt
    21901 tttccactaa aactttgaca ttagggccag catccatcgt aaagtagcat ggaatgcctt
    21961 gctttctcaa ttgtcgcacc gcatccatcg cttttaagct gtcaggagac cagtaagtga
    22021 atggaggctg ggcagctaaa gtcgttccat gcatttttag tccgtttctt tccatcgttt
    22081 ctccgagaag ttggaaatct tttgttttga ttgcttgttt caattgatat agatcccctt
    22141 caacggaatc taaccagcct tgataaaaac tggatgtttc gactgtccga cgcatcccat
    22201 ttctgctgga cacttctttt ttctggtcgt tgatcaaaac gaaaaccatt gctaaatcgt
    22261 cttcgaaaga atcggaaggg actggcttag cgtaagaact taagtcgtca tggccttttt
    22321 cccattcgac gaaaccaccg aaaatacttc ggcaagctga accagacccg cgtcgagcaa
    22381 atcttgaaag agagagatca tctaatccaa gcttaagagc agtattgcaa gcgcctgcta
    22441 aagcagctag cccgctggca gaggaggcta atccagctgc ggtaggaacg aaattctgac
    22501 tgattactga agcttttagt gagaggccgg cttctttacg aaaaaggtca agaaactgac
    22561 tgactttttt tgttgctttt tcgtcttgca aggtgccatc tagataaaat tcatctacca
    22621 tatagctatc agaaaagatg acttctgtct ctgtataaaa tgcatccaat gttaacgaaa
    22681 ggctgttgtt cattggaagg atgagttctt catttttctt accccaatat ttgattagag
    22741 caatgttggt atatgcgcgt gctttgcctt taaacatagg tatgtactcc taatccttgt
    22801 atccaagttt cagcagcacc cgcatcttca agtgcttgac tgatttcttg ggcttttgtt
    22861 tttgtttgtg ctaatgcaat catacagcca ccgcgtccac cgccagttaa tttggcaccc
    22921 aatgccccgg tatcttttgc tgtttggatc aatagattaa gaaaatcatt gctgattgtc
    22981 agcttttcca gaagtgattg tgattcatcc attgtctgtg ccaacgtttc tggtgaattt
    23041 tcaatgattg cttgttttgc ttgcttcgtc aagtatccta atttttggat catttgtcca
    23101 gtttcatgag gctgggtttc aaagaggtga gcgacatctt tgacggcttc tcttgtttgt
    23161 cctttgattc ctgtatcagc gacaatcaaa aaagcatcga tgttcaaaga aaagtagtcg
    23221 aaaggatgcc cttttgtaaa ataaatcggc tgcaagctgc tagtagcggc tgcatcgatt
    23281 ccactaggat taccgtgggc gattttttcc gaaagctgga cattttctaa tagtatttca
    23341 cgagacaaag gaaatgctaa gtaatcataa aaagcacgag tgactgctgt ggcgactgca
    23401 gcgcttgatc ccattccacg ttcagcagga atcgtgcttt caatggtcag ttgcaagttt
    23461 ttttggatgt catgctgttt ttttaagtga atgaataatt ttttgatatt ttttaatgca
    23521 tgaggagctt cggctaaatt tcctgaatag taggaagaaa ccagttgatc gtccattgca
    23581 tccagctctt caaggaatgc ggtgactttt gttgcataga aaggaaaggc aatcgctggt
    23641 tctccataaa caaccgcgtg ctcgcccatc aatatgatct ttccgcttga ttctccttgg
    23701 ccatagtttg ccataatcta actccttctc acgtaaaagt gatttttaag caaaaaataa
    23761 agcgcccaaa acgagttgcg ctgcttcacg aatttttttg tccactaagg gtctcaaaaa
    23821 acattcctcc tttattttac taaaaccatc tcagaagtaa aactaagaat acgttttcta
    23881 aagaaaaaac acttttttct taagaatttt ttgacttttt ttcttctttg actagaaaaa
    23941 aatacaattg acactactag atattggggg tatatttaaa aaggttggag ttaaacacaa
    24001 tatttagtga tgaagtgagc tggaggtaaa tgagcgtgat cgttttagca ggtacaattg
    24061 gtgccggaaa atcaagttta acagaaatga tggctgaaca ctttgatagt caggcatttt
    24121 atgaatcgat tgatgataat gaagtcttgc ccttatttta tgcaaaccct gaacagtatg
    24181 cttttttgct gcaaatttat tttttgaata aacgttttgc aagcatcaaa caagcaatga
    24241 aagatgacaa taatgtattg gaccgttcga tttatgaaga ttcactgctt ttccatctaa
    24301 atgcagatct aggaagagca acagaaacag aagttcgtgt gtatgatgaa ctgctggaaa
    24361 acatgatgga agaacttccc tatgctgccc ataaaaaaca tccggattta ttagttcata
    24421 ttcgtgtatc ttttgatacg atgttggagc gtatcgaaaa acgtggtcgt tcgtatgaac
    24481 aattgtcctt tgatccaagt ctatacgatt attacaaaga attgaacaga cgttacgatc
    24541 aatggtatga agaatacaaa gaaagtccaa aaatccaaat cgatggagac cgctataatt
    24601 ttgtggaaga tcctcaagca aaagaagaag tcttgaaaat gatcgaagaa aaattagcag
    24661 agattcgcca aaccaaagta gccatttgat ttacagttgc tcaaataaag ggtaaacctt
    24721 tatttgagcg acttttttct ttatactgga aacaaatgtc aggaggatag agtatgcggg
    24781 aaaaacacta ttttaatcaa gaccgtaatt tttggatcaa tgtacagcaa aacgatgtgc
    24841 tagctgttaa aaacttaaaa gaagaatatg gcattagtga cgagatgctg acttattctc
    24901 tagataaaaa tgaacgggcg cgcgtagagt acgattcttt tgatgaagcg ttactgcttg
    24961 tatcaaatgt tccgcaccag caaaaagtcg ataatcatta tgaaactagt cctatcgctt
    25021 ttattttaaa agaagacggt ttgtttacat ttaccacgcc gaataccgag tacgtgattc
    25081 gactaattcg atcgcttttg gaacgaatgc cagacatgag tgtatacagt ttattgtttc
    25141 gtacattgtt tttgatttct gattcctttt ttcctttaat cgaggaagtg aatagtgaac
    25201 gtcagcggct taatctgaaa ttaagagaaa aaacaacgaa caagaatctg ctgcaacttt
    25261 cagatttaga gatcggtttg gtttacttag ttacaggaac aaaacaaaat gtagtacttt
    25321 tagaacaaat caaggcattg gctatttatc gcaagttgtc agaaaaggaa aaagaagagt
    25381 tggacgatgc gctgatcgaa gcacgacaag cagttgaaat gacgaactta gcttcgcaga
    25441 ttttagacca gctctctggc acttataata atttgctgaa taacaacttg aatgacacga
    25501 tgaaattttt gacggtctgg tcgctgatct tgaccgttcc aactattgtg acaggattct
    25561 tcggaatgaa tctccagtta ccctttaccc attccgtttt cggatgggga atcgccttga
    25621 tcatcagttt ggtcttatct atttggatgc ttattgcact gtggcggcgg attcgttgat
    25681 ctagtctttg atctgcaaac caagggcttg ggcgatcaca agtgcttgct cttggttgac
    25741 gattagtccc gccaaagaat cttgagataa agcgattgct gtgaagaaat tagtcgtaaa
    25801 gtctaatccc ttcagagaag ttcgaaacca gttcgaaccg gtcaactgat tgtcagacaa
    25861 aaaaaggttt ttccaagtga cctcataaaa ttcgctgtct tctaattgac aatgatcgaa
    25921 ggagacagct ttcaagtttg tactactaaa ggaagccatc gaagcaatac aatcagtgaa
    25981 agtacagtct cgaagataac tttcggcaaa attcgtacct gttaatttac attgatgaaa
    26041 cacgacttgg tggaaactgc caccaatcca ctccagattg gaaaaatcac atttttcaaa
    26101 aatgacatta ctgcattcaa aatgacgtaa aactgttttt tgcatcgtaa gtttttggat
    26161 atgactctct cttaatacca tatttttggc atcgagatag ctttggtcac ccccgttgat
    26221 cagaagacca gagaagatct cctcatcttc taaataaaac tctccctttg taagaagagg
    26281 aagagaaggt ggaataggtt cgattttttt catgtgggtt cacctcgttt ttctttagta
    26341 tagcaaagaa gcgggaatca ctgctagaaa gtctgctaat ctaatcattt tcagaaaagg
    26401 aagacaaact gtatgaaaaa cattgacaaa aagattagtt tttgctatac tatgatggtt
    26461 gagaaataaa agcagaagca cccgcttctc gccttagttg ataatgtcac tgggctagat
    26521 agttttcact ggttctatta ggtgaagtac gcgcgggttc tgtaagagaa ctcgtttttt
    26581 tattgccgtt tattcggtaa gcttttcttt ctctcataat atttggaggt gaatgaccat
    26641 agcaaaggat atgatggtta acgacggcat tcgtgcacga gagttacgat tgatcggttc
    26701 agatggtgag caattaggag ttaagacaaa agcagaagca ttgaatattg cagaacaagc
    26761 aaacttggat cttgtgttag ttgcccctgg tgcgaaacca ccagttgcgc gaatcatgga
    26821 ctacggtaaa taccgtttcg aacaacaaaa gaaagaccgc gaagcgcgta aaaaacaaaa
    26881 agtgatcaat gtaaaagaag ttcgcttaag tcctacgatc gacttgaatg acttcaatac
    26941 aaaacttcgt aatgcacgta agttcttgga aaagggcgat aaagtgaaag cttctatccg
    27001 tttcaaaggc cgtgccatta cccataaaga aattggtcaa aaagtcttga atcgcttagc
    27061 tgaagaaact gcggatattg caacagtgga acaaaaagcg aaaatggacg gacgcagcat
    27121 gttcttgacg ctggcaccga aaaacgacag taagtaattt gaatgactaa ctggctgttg
    27181 gacagccgat agtcggataa cgaaagattt tgaggaggaa atatagtcat gccaaaacaa
    27241 aaaacacacc gcggatcagc aaaacgtttc aaacgtactg gtaaaggcgg attgaaacgc
    27301 ttccgcgcgt ttacaagtca ccgtttccac ggcaaaacaa aaaaacaacg ccgtcaattg
    27361 cgtaaagcaa gcatggtttc tagtggcgac ttcaaacgta ttcgccaaca actagcaaaa
    27421 atgaaataag aagtacaaat aacagaatct gaagatttat ttttattagg aggaattata
    27481 catggcacgt gttaaaggtg gaacagtaac tcgtaaacgt cgtaaaagag tgctaaaatt
    27541 agcaaaaggt tactacggtt caaaacacac tttatttaaa acagcaaaag aacaagtaat
    27601 gaattcatac aactacgcat atcgcgatcg tcgtcaaaag aaacgcgact tccgtaaatt
    27661 gtggattgct cgtatcaacg cagcagctcg tatgaatggc ttgagctact caaaattaat
    27721 gcacggtttg aaagttgctg aaatcgacat caaccgtaaa atgttagctg acttggctgt
    27781 taacgatgca gcagcattta ctgcattggc tgaccaagca aaagaagctt tggctaaata
    27841 agctaaacaa ttataaagag gaaagaagat aacgaaaagt tatgttcttt cctctttttt
    27901 tatttacaat cctccatcaa atgaaacagg acaataacgt ataagtctaa aatagcataa
    27961 ttaaggtttt ttaattgaaa actgaacatt tatggtcacc ctttaatgaa agcgctaact
    28021 acataagtta gaatgagttt gactgatata ttagtttgct tatcagaaaa acaaaatgga
    28081 cggaggaata acatgagtga tattattatt ggtattttgt tagttatctc attctttttc
    28141 atggtctggt actgtgtaaa aggctacaac ttgatggtcg gctttgccat catggctact
    28201 gtatggatgg gattagcgct agtcggaaac acgttttcac caaacccggc gatggaaggg
    28261 caaggagtaa ttgatgtgtt gacgcatatc tatacgacgg ggccagctga gtacgccaaa
    28321 tcgatcttgg taaatgtctt ctttggtgcc tttttcgggc gtgttctagt agatagcgga
    28381 atcgcagcta ccctgattcg aaaggtagtt gaacttggag gggacaagcc aagaatcacg
    28441 atgagcttac tatgtattgt aaccgctgtt atattcatgt cgatgacagg aatcggacca
    28501 gtgatttcta ttgcagttat tgtgttgcca atcttgatgt cattaggtat ttcagtaccg
    28561 gttgcactgt tctcatttat gggctcgatt atggctggaa tctttgctaa tatcgtcaat
    28621 ttcaaacaat atcaaacgat ttatgctggt ttcaatccag cggctgaaag ctatacttat
    28681 aatgactatt tccaaattgg tatgattggg atgattgtaa gcttagttgt ggtactaacg
    28741 gtcgctaata tatcaatgaa caaaaagaaa cgctatgcaa tggcagcaaa cgtaccagct
    28801 gaaggtggcg acgcaccaat gatctcatgg ttagccgtat tattaccagt tttaggggtc
    28861 gttcttttag atcttccaat catcttaggt tttattctag caggtatctg ggcattgctg
    28921 tttactggaa aattgcgcgg aggttataaa gaaatctgtc gtcagtttgc gaaattattt
    28981 acagatggcg cagtagatgt tgcaccaatg gttggtttct tgatgacctt agcgatgttc
    29041 aataattcag ctgcgtatgc ttctccttat ttttcagcta tttttggaga ttggataccg
    29101 caatcaccat tagtcttggc aatcgtattt gctatattga caccactagg atttttctgc
    29161 ggtccaatga atctagtcgg ttcaggctca gctattttag ctgtcgtatt agctgttaat
    29221 ccaacgatgt cgccagcctt tttgttcccg ttatttgcaa tcacaacgat tgctcctcag
    29281 catttagata ttacacaatc ttgggttgct tggggattag gttatacaaa agtaacttct
    29341 cgagaatata tgaagaaatc catcccaact ggttggatca tcggagcgat cttatgcctg
    29401 atcacgttct tattgtacgg gaacgcctag taaacaaaac gtcgataaaa acgattagat
    29461 gaatggagga aaagaaaatg aaacgttttg tgcgaatggg aattgatgtt ggcggtacac
    29521 atacgaaagc agtcgcaatc gataatgcga cgcacgaaat catcggaaaa tcttcagtga
    29581 aaacaaccca cgatgatgtc cgtggagtag ccgcaggtgt cgttcagtct tttcaaaatt
    29641 gtttgcggga aaataatatc agtccagaag atgttgtgtt tgttgcacat tctaccacac
    29701 aagcgactaa tgcactgatt gaaggagacg tagcaaaagt cggcgtaatc gggatggcaa
    29761 aaggtggatt agaaggtttt ttagcaaaac gacaaactag attgaacgat atcgatttag
    29821 ggaataagaa aaaaatagag attgtcaatg cattcttacc tgtcaaacac ttaaatgtag
    29881 accgtgtttc agaaacgatt tcttctttag aaagagaaag agcggaggtc ttggtttctt
    29941 ctatggcatt tggagtagat aatggggaac cagaacgagt tgtttatgaa gcagcatcgg
    30001 taaaatcgat ccctacaacg atggcttctg acatcacaaa attatatgga ttgacaagaa
    30061 gaactcggac agcagcaatc aatgcatcta ttttaccaaa aatgctagat acggcaacct
    30121 ctacagaaga ttccgtacga gaagcagggg tcaatgtttc tttgatgatt atgcgcggag
    30181 acggcggagt aatggaaatc aatgaaatga aaaaacgacc tgttttaacc atgctttccg
    30241 gtccagcagc atcagtcatg ggctctttga tgtacttgag agcgtctaat ggtgtttatt
    30301 ttgaagtagg cggaacaaca accaatatcg gggtcattaa aaatggacgt ccagcaatcg
    30361 attattcaat tgtaggtggc cacccaacat atatctcttc attagatgtt cgggttctag
    30421 gtgtggcagg cggttcgatg gtacgggcta atcagtcagg aatcatcgat gtcggtccac
    30481 gttctgcaca tattgccggt ctagattatg cagtatttac ggagacagaa aaaatcaaag
    30541 gtcctaaggt tgaatttttc tctcctaaag aaggagaccc tgctgattac gtcaaggtag
    30601 tgatggaaga cggagaagaa gtgactatta caaatacatg tgcagccaat gtccttggat
    30661 tagtgcaaga agaacatttt tcttatggaa atgtcccatc tgcacgaaaa gccatccaag
    30721 cattagctga ttattgtcat acgacggttg aagacatcgc agaacagatc atggaaaaat
    30781 cctatgctaa gattgaaccg gtgattttag aattggcaga caagtatcac ttggagaaag
    30841 atcagatttc tcttgtaggt gtagggggcg gcgctgcctc attgattact tactttagta
    30901 ataaaatggg tgtgaaatat tcgattcctg aaaacgccga agtgatttct tctattggtg
    30961 tagcacttgc aatggtccga gatgttgtag aacgtatcat cccttcgcca agtaaagaag
    31021 atatacgatc gttgaaaaat gaagcgatga ataaagcgat tgaatcaggc gctacaccag
    31081 aatcgatcga agtccacgta gaaatcgatc cacagacttc aaaagtaacg gctatcgcta
    31141 ctggttctac ggaagtgaaa gcaaccgatc taaccaagga gatcacaaca gaagaagcac
    31201 tagagcttgc tgcagaggat atgcgattaa ataaaaatga agtttgctta ttggaaaata
    31261 cacctttctt ttatgtatgc ggcgaacaga atcgttcaaa aaatgctgga agtcttcgga
    31321 tcatcgatca aaaaggattt atcaaggtcc aacgtggaca tgcttcatgt atgaaaacaa
    31381 ctgcagccaa ttatatgaca gctgtcgaac aactttggga agatatggca gtttatcaaa
    31441 ctgaattgat tgcccgacca gaattttatc tctgcttagg agcaagagtc tctgatttta
    31501 cagcgacgga tctggaacaa ctacagcttt tgatggatct ggaagtatct actatggaac
    31561 cggaggaaga agtgatcgtc gttgccggaa atatcaaaca aacataagtt agaagagtta
    31621 ctaaacgaat tacaacagat ccccgaagaa atttggggat tctaccagtt tcggcgagat
    31681 ttattttgga aaaagattcc tttgtccaag cagaaaattc ttatacaaca atcaatcgat
    31741 tgtggtatcg aaacggcatg tagcatcaaa aaaaagtacc cttttgccga tgtaggagaa
    31801 atttgtgaac aaatggctgt tccaatcgtt tcctgtgaat cagagcaaat aaacgaacga
    31861 atcacttttg caacttatgc agaagatgaa gggattcgat tgatgacgga gccgttagaa
    31921 aaattaaaat gttcaggact agcttcgatc tctaaagaaa cagcgcaagc tttgattatt
    31981 ggacatgaac tatttcacca catcgaagct tctgttaagg gaatttatac gcaaagcgaa
    32041 aaaattgtgc tgtggcggtt gcctttttat acacatcaat caaacattcg tgcattgagc
    32101 gagatagcag cgatgagttt ttcaaaagag atgaatcaaa gcgatttttc gccttatgta
    32161 ttagaggcag tcttactttg gccatataat gaaattcgta gccaagggat cttagaagaa
    32221 ataaaaaaaa tagaaaaaag atgtacggag tatgatttag attacaaata aagagactgt
    32281 gacaaaagtc gtgtcacagt cctttttttc cgaataaacg atgttctcct gtcaactcct
    32341 cggtcacaag tcaaaatatt caacaaacat gagaagctgt tttttgaaat tgttccttgt
    32401 gactcggaga tgaacggcag gctcacgacc tcttggaaaa cggtgttcaa aagcttgcat
    32461 ctgcggtaat aagaacagcc aagttaaaaa cataagaagc tatttttact tggctgttct
    32521 tattacttga tgcagggcag ctttttcaca acctctcaat aaacggtgtg caatcagctg
    32581 accctcggta ttagctcaaa aaaaacggca aaatatgagg aactattttg ccgtttattt
    32641 ctctaatact caggtcaaaa cgctgattcc aaaacctctt atttgatggg tctgtcctga
    32701 atatcttcaa aataatcggg gtaattatga atcacgtatt cttgctcctc gataatcata
    32761 tgtaaatgca tcgtgcaaag aaatccgact tcatcaaagt tatgatcaat cattgattga
    32821 agcattgtct gatgttcatc taatactcga ccccagtcta attctgaaat cgtcaaacgt
    32881 aaccatctga aacggttcaa gtgcgtacta taactttcta gccaatccca aatttctcgt
    32941 tttccagcaa tttcataaca agttcggtga aaatcagtat ccaaatcaaa aaaagctttt
    33001 ttattttgag cgatcactgc ctgatcggtt tgttctagta ttttttctag tttagcttga
    33061 tcttgcttgg tcattttcgc actgcattct tgcaggatcg gtttttccaa cttttcccgc
    33121 gtataacggg ctaatgaagc agatcggata tcgatttttg aaacataagt gccggactgt
    33181 ggaatggttt ttacaagctc ttgattgcgc agttggatca gtgcttctcg aatcggtgta
    33241 cgcccgatag ctagttgttc ttctaacact ttatcggaga ttttacttcc aggtgctaac
    33301 tcggaataaa tgatttgttt acgtatagat tcataagctt gctgttgcaa attattgata
    33361 accatttaac cttcctttct atgcagatat aatctattat aacataaatc gcttacaaat
    33421 cgtgtgggca taaaaacaca gtctgcaaaa aaagccctga taggcatgcc tattagagcc
    33481 tcgaaaagaa aaatattgaa tcaatctggt cgtacctctt ggatcggttt gacacgaccg
    33541 ccgattcttt tagcaaaagc ttcggattct tttttgtctt tgaaaatgag cagattgtct
    33601 aagttttttt cccgagtgcg acagccagtt ttatcgatgt aaccgtgcat tactttaacg
    33661 acatacatgg ggatcacctg cttttttctt ttcattatac gaactactcg acacaataca
    33721 agaaaaatgc ttagacggta tttattaaga atttacatca tccaaagaag cgtacgcttg
    33781 gaataactgt tctaactccg gattgacttt gctcagcttc actaatcgtt gcgaatcaga
    33841 aagtaaaaaa cagtgtttac tactgccagt agtttttaat tttttgtctt cagttcccac
    33901 aatttcgtag ctaaaatcca accgtgttcc cgtatacttt tggatagttg ggtggatgct
    33961 cactttttct ccgtaatgga tcatttcttt atagctgcaa tttacttcca gaacggggat
    34021 gatgatccca gattcttcga tttttttata gggaaaccct agatgtacca ataaggctac
    34081 ccgagcctct tcaaaccagc ggatataatt gctgtgatga ataatcccca tttgatctgt
    34141 ttcataataa aaaggttctc gcacataacc tgtaaatttc tccatgactc atgcctcgtt
    34201 ttccattgat tttttacaaa aataattcat ccacttcgga agtgcttcgc tgatttgtgt
    34261 cggcaaaaca atatattgtt cttttgccag ctggtctcca atgaaactat gaagataaac
    34321 agctgctgca atcgtttggt cattttgagg aaactgggct aaaaatcctg tgatcatccc
    34381 agctaaagtg tcacccatgc cgcctgttgc catagcagca gaacctaaag gattggtgta
    34441 gaagtgctgg tgaccataaa tttttgtctc atgacttttc aaaacgagca cagcaccgat
    34501 ttcctgttgc ttctttttgt tttctttatc ccgttgttct tgtatcggta gagaactcaa
    34561 ccgttgccac tccatttgat gaggtgtaag tacagtttgt tcgggatagg gtagcaaaag
    34621 atgattagca acaagtaaac ttaaagcaga gccatcaatc accagccatt gttcttttga
    34681 ttgtttttct agtacttgtt tgaaacgttc caaactctta gaagaagtac ccaatccggg
    34741 accaatcaag agtacatctg cttgctttat tacggaaaga aactggtcag tatccgtcca
    34801 atctacaatc attgcttctg gcagatgact gtgaagagca ctccgatttt tttcatcagt
    34861 tactacagtc gtgagacccg ctccactgtg tacggcagct aaagcactca taatgattgc
    34921 tccgccatat tgttcattgc cgccaatcaa aacgatacgt ccaaatgttc ctttatgtga
    34981 aatctttgga cgaggtgtga tgactctttt cactagttgt tcgtttatca gcatcgaaaa
    35041 acctcctttt actcattata gcgtatattt tttttagaga aaaacagata agaaaaggaa
    35101 ttgtcagatt ttttgaacta tttttttctg taatttgtgg tatcatgaac acgaagactt
    35161 aaataagtgg aggattaact ttatgacaat tgattggcaa aaagaagtag aagcacgtaa
    35221 agaagacttg cttgaagact tgaaaaatct tttacgtgtc aacagcgagc gcgatgattc
    35281 aaaagtaaca ccagatgcac catttggacc tggacctcgc gacgcattga aacatatgtt
    35341 agcttatggc gaacgtgatg gatttaaagt aaaaaatgtc gataactatg cagggcatat
    35401 tgatcttggc gaaggagacg aaacgctagg gatatttgga cacatggatg tcgttcctgc
    35461 aggagatggc tgggacacag atccttatga accagtaatc aaggatggaa agatctttgc
    35521 acgcggatca agcgatgaca aaggtccaag tatggcagct tactatgcga tgaagatcat
    35581 taaagaactt gacttgaaac tatcaaaaaa agttcgcttc gtagtcggca gcgatgaaga
    35641 aagcggctgg ggcgatatgg cttattattt cgaacatgaa gaagaaccag attttggttt
    35701 ctcaccagat gctgaattcc caatcatcaa tggtgaaaaa ggaaatgttt ctttagcttt
    35761 acgcttcaaa ggcgacaatg caggagatta tgtcttgaaa tcttttgttt ccggacttcg
    35821 cgaaaatatg gtgccaggaa cagcaactgc agcacttcaa gtaccaagtg cagatgcagc
    35881 tatcgcgatg gaagaagcat tttaccaatt catcgaagca aatccagtaa gcggaacgat
    35941 tgaagcggac aatacgtatg taaaaatcga attagttggt aaaggcgcac atggtgcaag
    36001 cccacaatct ggtatcaatg caggttcttt cttggcattg ttcttagata actatgaatt
    36061 cctaggatct gctaaacaat ttatccatgt agcagctgct tatgtccatg aagattttta
    36121 tggagaaaaa ctaggagtcg cttatgaaga tgaaaaaatg ggcaaattga caatgaatgc
    36181 tggactattt gcttttgaag aaaatggtac agaggaagcg aacttcatca atatgaattt
    36241 ccgttttcca aaaggtgtaa cagtagacgg attgcaaagt gacatcgaac aaacagtggg
    36301 gcaagaaggc gcaacagtta ctcgaggtgc tcgcgtaatg gaaccacatt acgtaccaat
    36361 ggatgatcca ttggtggcta cattattgca agtctatgaa gaccacactg gagaaaaagg
    36421 ctacgaacaa atcatcggtg gcggaactta cggacgtttg ttgaaacgtg gcgttgctta
    36481 tggagcgatg ttcccaggat atactgatac aatgcatcaa gcaaacgaat ttatgagtct
    36541 tgatgacttg ttcagagcaa cagcaatcta tgcagatgcc atctatcgtt tggcgaaata
    36601 aaaatactaa aaaactccgg ctatctctga cagctggagt ttttatgttt aaaaatgaat
    36661 tagtacatcg gtattcttaa aagtaattta gttcgtcttt tgatttctca ggagggatca
    36721 aaaaggcgaa agcactaagt atagcaaaac ttgtgatgat ttgcgtacga tacgtaaacc
    36781 aataatgaat ctgtgagtga ttagctaaaa tattgtacca aatatacggc gttacagcag
    36841 ctaaaacaaa caaaactaga agttggaacg gacgccagct atttcgttta cgataagcaa
    36901 aatagagaaa aacaagacaa gtaatcaata gcaataatat tgctactttt ggatacatca
    36961 atctaatatt gcttttcagc atctctaatc gatgcaacgg gtatttgtcg tttccttccg
    37021 ttcgaaagag gatttggttg attgcatctt ttacaatgtt tttgttcaat atgatcgatg
    37081 ataggatcca tttagccacc catgtcaaac catagcctaa tccccaaaag ataccggtaa
    37141 aaacaaaact gcctacttgt ttgctcaatg aactgcgttt atccattaaa tacttatttg
    37201 ttacataata aataatgatc acaggtatcc cccaagagat gaccggtgtt gtcaacaagt
    37261 caaaaaagtt agctaaagaa ccaatgacaa agaaatacaa gaataattgt tggttggtgt
    37321 tggagagttg tggttttgtt aaaaatagta atacagacaa taaagtcaaa atccaaatat
    37381 ttgaaaattg catactgaag aaaaaggttg cagcatttcc tatagcaagc gaaatcaaca
    37441 aggctgtcgt taaatagatg tcgctacact tggttaaaaa gtagctcgtc aatccaatca
    37501 gcagaaaaag aatggctgcg taaatcattc gaatactccc caaactcatc cctagcagta
    37561 aaggtcgtaa aaaaagctga taaccatgcc aataacgggg ataatccgcc atagacatcg
    37621 cgttctccaa ggagttcaat tcaggatctg cttttgtttt tcgtatcatc aaatgatcag
    37681 taaaattatc taaccgagtg cctagcatat ttccttgatt gatagaagga tacattcctt
    37741 ctttttcaac catctgtaga gatttttctg cattcgattc aatagagtgc tgcggaatga
    37801 aaaaagtaat tgtcagtagt acgcaatagc tagcgatcaa gagaacaaaa gcaaaaaata
    37861 tctttttaaa tagtttcatg agtatctcct atatctgttt tcattttttc tcttttatct
    37921 agtaaaaaag gagagaggat ataaacaggt agacctaatg ctaaaacaaa cacataccgt
    37981 aaactaaaag ccacaggagt agcaatcata atagtagcaa aaacaagcat accgggaaga
    38041 aaaaggagta aataccgata ttttttctta aagattgcta aaagtgcact gccgatagct
    38101 aaccacagta atgtgccgga accaaagaaa tcacgagcat cgatgatttt tttcattcct
    38161 gacaatccag taagatgttc aatcaaatct acttgcttca taccataatt attcgtgttt
    38221 acacgagtat ctaaaaagcc gtaactattt tttgtaccag gtacccagaa cccgaacgtt
    38281 tctaaaatat aagcatctgt atagattttt atattacttg gtaaatttct tgcccatagt
    38341 gatagaaatt cttttttatg tgtattcaaa aactcattgt taaaattttt atgccacttg
    38401 attgagtctg caagactagg agaatacgct tcataatctt ttatcgggag tagctgattc
    38461 atgaatgctt tatctttctt agacaagtca ccatccataa caatagttct agaaatttgc
    38521 tgcaaaggaa cacctaattg ttcagcagtt tctgtaggaa tagcataacg agccatcaac
    38581 gggtggatga gtagtccagc aatcactgta taacccagca ccacttgaaa gaatgcttga
    38641 tacctttttt gtttcaaaat aatcaagccg caaatcaaac ctgtgccggt tactacataa
    38701 atcccattat ttcttagata ggtggtcagt aaagcaagga tgattacgta aatctgattg
    38761 agaatcgttg gcttttctcg ttgaaaaata aagtcaaaaa ccgctaagga aaataaaaag
    38821 caagcgatag agaacaaagt atctttttgc atgttcaagg cgtagattgg aaaaaccggt
    38881 gcaaatgcaa aatagcataa actaatgagt accggaaaaa aatggatgtt tctttttaac
    38941 agccagcaaa gagaataact tatcgctgaa gctgttatca atgtttgaaa aatcgaaaat
    39001 aaaaatacac cgtgattgat cgtatcagtt ggtaaatcat gaacaaatag cttgaccagt
    39061 aaggtgaata agactggatg atgattgctt ataggaacat cacccataga ttgggcgacc
    39121 gaacttaacg aatcaggcaa aaccactcca ggatacaatg ccaacagata aggaagccaa
    39181 cacagaacta aaaagacagt aaagaataaa aaatattttc ttgaagagaa agcctgtgtg
    39241 ttttgagcag taaaaaatgt ttccagtttt ggcattgata aataaatcga ttgtaaaaat
    39301 aaaaaagaat acacaaaaat ggagaaataa acaagaaagt aaaaaatact tccatcagca
    39361 atcgttgtct ttgtatagtt tgctgagaca tactgaaatt gtatctgtct gccaatagaa
    39421 agtgtaagag caaaagctgc agaaaaaaac aatcgggcaa tagctgcaac tttgtttttt
    39481 attgaaaaca actctattcc atattgctta aaagcaagta gcaaaaccat ggctaaacca
    39541 atgattactg ttggattcaa agttcctaga ccataagaag taaaaaaagc aaaacctgac
    39601 gcaaataaag ttgcaaaaaa agtaggaaat agattctcct ttttcttatg cataaaaaag
    39661 gcctcctact tcatttcttt attttgatat tcgtggtaga catggtataa attcaattca
    39721 tattctttct tggcatttgt gacgaccgta tccaaaatca accctgttac taatgaaaga
    39781 agggctaaag tcatcaatcc agtacttaaa atcgcagaag ggatgcgtgt aataaagctt
    39841 gtcagcatat attcacgaat cacaggaatc cccatgacca atccaaataa aaagaagaag
    39901 atggtccaga tcccaaagaa aagcaaaggt ttataatctt tgcacatttt gatgatcatc
    39961 atgatcactt tgaatccgtc agagaatgta ttcaattttg attcgctacc ttcaggacga
    40021 tcgcgataat cgattggaat ctcaacgaac ttgaattttt tatctaaagc atgaatcgtc
    40081 aattctgttt cgatttgaaa accagaactc ataataggga agcttttgac aaaaattcga
    40141 ttgaatcctc gatagccggt catgacatcg cgaatttttg ttttatataa gtaagtaatt
    40201 gtatttttaa ccagattatt cccaaaatca tggaaaggac gcttattttc atcaaaatag
    40261 gttccatttg atagacgatc gccaatgacc atatctgctt cgccagaacg taatttctca
    40321 agcaaaccat gtgcagcttc tgcaggataa gtatcatcac catctaccat caaataataa
    40381 tcggcatcaa tatcaaaaaa catttgacgg attacattcc cttttccttg acgtggttct
    40441 ttctttacga ttgctccgcc ttgaacggcc aattcgtatg ttcgatcggt cgaattatta
    40501 tcatagacat aaatatctgc ttctggtaat tcccttttaa aatcttcaat aacagttgaa
    40561 attgtcgcct cttcgttata acaaggaatg agcacggcaa ttttttcacg tgattccatt
    40621 tgtcactctc cttatataat aaaactaatc ttatttcaat aataccgtat aataactaca
    40681 aatactagct attccaagag gaaatagccc tatttttata aaaatcaccc actataaaag
    40741 aaggatagga tacctctctt ttagatagtg ggtgcgtatt cgttatttca tttttttgac
    40801 gtattaatct attgcgccct tttttcttga aatgtctaat acggttcctg tatgagcatc
    40861 tgctaaaaat tcataataaa cagtttcgcc atcttctatc cgggaaagcc caccccgata
    40921 agcactcgac cgaacggcga atttccgaat aggttctttt tcaaaagaga tccaagcacc
    40981 ttcgattgcg ccttctttta aaaaagatgt tttgatattt tcaagcactt ggtctgctga
    41041 taacactttt ttgcgatgat accaaatcgt tgaagctacc ccactcaata atcctagacc
    41101 gatcccaacg gctaacccgc ctttaaaata gcttaattcg ctttcttcat acatgtggat
    41161 atacctcctt cttctcactt gctactcttt gtgtttattt taccacactc tcatattttc
    41221 tttgaaataa ataaccgcta ccagaaaaaa taagctataa taaatacgat cggtctgcag
    41281 aaaaaatcat attttatccg cagatcaata taagtctgag aggagtttag tctgaaattt
    41341 agacgaatag catatggaag aaaaaacatt tcaacgaatc aaagagttaa cagaattaca
    41401 aggaacaagc ggttttgaac aagacgttcg cgcctatatg gaaagtcata tggaaccgct
    41461 ggtagatgag ttacagttag atggtttggg cggcattttt gggctgcgcc accatgaaga
    41521 agcggatgca cctcgtgtaa tggtcgcagc acatatggat gaagtcggat tcatgctgac
    41581 acaaatccaa gaaaacgggc tttttaaagt cgtacctcta ggcggatgga acccttacgt
    41641 tgtttctgct caacgtttta cacttaaaac ctctactggt aagaattatc catgtatttc
    41701 ttcttccgtt cccccacatt tattacgagg aacaagcgga caaaaatcag tagaagtaac
    41761 agatattttg ttcgatgctg ggtttgaatc aagagaagag gcaatgagtt acggtgttct
    41821 tccaggagac acaatcgttc cttacgctga aacgatcaaa acagccaacg ggaagaatat
    41881 catcagcaaa tcgtgggaca accgctatgg ctgtacaatg gtattagaag ctttagaagc
    41941 gttacaaaat gaaacattag gtcatacatt gattgcaggc gcgaatgtcc aagaagaagt
    42001 aggcttgcgt ggttctaaag cttctgtcaa taaattcaag ccagatctgt tctttgcagt
    42061 agactgctct gcagcagatg acacagtgac gaaaaatggc acattcggac atttaggaga
    42121 aggtacattg atgcgtatcc aagatccagg tttgatcatg cttcctcgtt tgcgtgagta
    42181 cttgctagat atcgctgaaa caaacaacat cccttatcaa tactttgttt caaaaggcgg
    42241 tacagatgca ggtgctgctc atacacaaaa tgaaggaatc ccaagtacag tgattggcgt
    42301 agtgggacgt tatatccata cgcatcaaac gatgttcagt attcgcgatt ttgaagctgc
    42361 acgagaaatg ttgattcaaa cattgaaagg tttagataaa tcaacagtga acacgatcgt
    42421 atacgggaaa taacgcgata gtgaaactat aaaatctggc aaaagcaacc ttttgtcaga
    42481 ttttttttat aaaaagagga tcaatgtttg ttagttcgaa agaaaggtgt ataatttaat
    42541 tgaaaaatac ataaacagaa agtagaggaa aaagaatgat tatcccaaat agtttagaag
    42601 aattagcagg ctacgtagaa actggaaaaa gtgtattttt ctttactgcc ggctggtgcg
    42661 gagactgccg ttttatcaaa ccgcaaatgc ctgaaatcga aaatgacttt tctgattggc
    42721 aatttattga agtagaccgt gacaagtaca tagatgtagc cgctgaatgg aacatttttg
    42781 gtatcccaag ttttgtcgtg atccaagacg gaaaagaatt aggtcgtcta gtgaataaag
    42841 atcgtaaaac aaaacaggaa atcgaatcgt tcctagaaag tgtaataaaa ggctgacatc
    42901 tgttaggagg ggtcaagatg aatcaagaat ataagaacat tttagtaggt attgacggta
    42961 gtgaacaagc acttgctgct ttcaaaaaag cgttagaagt ggctcgccgt aataatggga
    43021 aagtatatgt agcaaacgta atcgatcagc aattttataa ttttatgggc tatgctccta
    43081 ttgatcaaaa tctagtcgat cagcaaactg aaacagccaa gcaaatgatc gaagaatgta
    43141 aagaatatgg aaaatctgtt gattacacaa atatagaagg aattattgca tacggctctg
    43201 ttcgtgaatc catggcagtc aaattgccaa aaaaatatgc aatcgatcta atcatggttg
    43261 ggcagtctgg actaaatgca gtcgaacgct ttatgacagg aagtgtagcc agctacgtca
    43321 tacgtcattc accttgtgat gtcttgatcg tttctgacga agaagaacag ccaagtaatt
    43381 aagcaagtgg aacttgtata acctttttaa tcaaacagta aagggactgt gacaaaagtc
    43441 ttgtcacagt cccttgttac gaataaatgg tgttccagaa gaagcttctt cggaaataaa
    43501 gcatgaaaaa aatctcatgc agtgaatgca attttttgtt aagataaatc tcagcaaagg
    43561 ataatggagg aaagaacatg atttttgctt ataacaaagc acacgtcgga gacactttga
    43621 tggttatcat agccgacgat aaaggaacag aaaacactgt cgaacgtaaa tggaatgttg
    43681 ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa ctttttccat atctccgatc
    43741 atctaacaat tgaagggaac ggccaagtaa gcttaacaga tgaagaatta gcgaaattga
    43801 atcgattgat taaagaagca ggattcgaag aacagcttga agctgatcat gaaccgaaga
    43861 tcgttgtcgg gtatgtaaaa acatgcgtcc cgcatcctga ctctgaccat ttatcgatta
    43921 ctgagacaga agtggacaat gggacagtat tgcaaattgt ttgcggtgca ccaaatatcg
    43981 aagctggaca aaaagttgtc gttgcaaaac caggagccat gatgccagat ggaatgatga
    44041 tctggccagg aactttgcga ggggtagcca gctatggaat gatttgttct gctcgtgaat
    44101 tgcatttgcc aaatgctcca gaaaaaaaag gaatcttagt attgcctgag gatgccgtag
    44161 tcggagaagt atttccaaaa taaagaggtt gtgacaagtc tttgtcacaa cctctttttt
    44221 attaattttc actattttga ttttgtttta atgccgattg atcgatcgtc aattcaacag
    44281 tagttgtttt ttctttcgag ccttcatagt aagtgatttt gacagaatct cccacttttt
    44341 tcttatacaa agcagattga agttctacac cagaagaaac ttctttgtca tcgatcttag
    44401 taatgacgtc gtattgtttc aacccagctt tttctgccgg tgtagctgtt tggacatttg
    44461 tcacgatgac accgttagtt acagatgaag gaatcttcaa gatttgttct tgttgctgag
    44521 tagaaacggc agataagtcg accattgtga tacctagagc tggacgtgtc acttttccgt
    44581 ctttttccag ttggttgatg atattcacta catcgttact tgggatggca aatcccattc
    44641 cttctacgct tacattagaa gtcgattcag aagtacttgc gatcttactt gagttgatac
    44701 cgataacttg tccttcgata ttcactaaag gaccaccaga atttcccggg ttgatcgctg
    44761 catctgtttg gatcgcattg atattgactg tttcgttcga ttcattcgta cttgttactt
    44821 gtcggttcaa ggaagaaatg attcctgaag tgacagaatt cgcatattca gatcctaatg
    44881 gggaaccaat cgcaatcgca ggttcaccaa ccttcaaagc acttgagtcc ccaaaagagg
    44941 caaccgtttc gactttgtct gaattgatct tcaacaccgc taaatctgaa taggcatctg
    45001 ttccaactaa ttcagctttt accttcgtac cgtctttcat catgacttcc aaaccttgct
    45061 gtccatctac tacgtgattg tttgtgacta cataagcagt attcccgtct tttttgtaga
    45121 ttacgccgct tccttcacta taagcttcca aattactatc atctgagctg ctactttctt
    45181 cttgctgccc gaataatccg ccaaaaccgc ttgattggtt ttgactttgt aagttgataa
    45241 cagaaacgac agcgtcttga actttatcta ccgctttggt gatatcagaa tctacattga
    45301 ctttgacatt ttccacaact gtttcaccgg cagaattttg attgccgctt gtagcagtag
    45361 aactattgtt gccagtgccc atcgctgcat aaaaaatgcc agctgtcagt agtcctccaa
    45421 cgatccctcc aacaagcccc agacccaact tacgccagag gctattattt ttattttttt
    45481 tcatctttgg tgtcacattt tttctatcca tgtgttcctc ctccaattac attcatttat
    45541 ctactgttag tatagtctta tttgttgact ttagtctact ttgagacttt gaacttttta
    45601 tgaaaaactt taggaaaaat cattgttttc agatgaaaat atttgatttt ctttttccac
    45661 agatattgtg taaaactacg cggataggat tggaaaaata agggataaaa catatccaca
    45721 gcctgtgtat aaagtggata aattcttata aaacgtatgt ttgcctaatg aatgtgatga
    45781 aataaggtaa aacaacgctg ttttctgtgg ataaacctgt ggatgttgtg cataactgtt
    45841 ggggaaatct ttttaacaaa aatagtcaaa ataaacatat agacgaaaaa attgattata
    45901 ataaacaaag tgagcgtgaa gatcaaagga gagaaaacat gaatatcaaa attatttcag
    45961 taggaaaatt aaaagaaaaa tacttgatcc aaggtatcaa tgagtatgtc aaacggctga
    46021 atgcctatgc gaaaatcgaa ttgatcgaag ttcctgacga aaaagccccg gagaatttaa
    46081 gcgaagcgca aatgagacaa gtaaaagaaa aagaaggaga acggatctta gcgaaaatca
    46141 aagaacaaga atacgtctat ggcttagcaa ttgaagggaa aaatcccaca agcgaatctt
    46201 ttgctaaaca aatcgaccaa ttaggtatcc aaggaaaaag ccaccttgtt tttgttatcg
    46261 gcggttcttt aggactcagc gaagccgtca tgaaaagaag caatgctcag atctcttttg
    46321 gcaaaatgac gtatccacat caattgatgc gcctgatcct cgtcgagcaa atctaccgtg
    46381 ccttccggat caacgcgggg gctccatacc acaaataatg atatttttta agagaaaaaa
    46441 ttatccctat tttaaataat agcaataggt agtggtaggg agaaatttat aaataacttt
    46501 tttatttgct ttggtagcca agtaagttgc aaaagaaaaa tagatactgt taaaaagata
    46561 atgtagacat agaaacaaat acgtaaaatt ttagttagaa taatggcaag gagaaaaaac
    46621 atgatagatg aaaaaaagtg tttaaaaaat ctattggagg atatggatga tattgaagaa
    46681 aaactatcta aatggactag gaaaacaaat atatttcaga tacttaagct atctaaaaat
    46741 gaaataagac atagtaattt tttagctttt ttgtttgctc caaatgaaac ccacaattta
    46801 tctgatgagt tttttaaaat gtttttgaaa aggtatatag attctaatga tgataccaaa
    46861 gttgctatca attattttga tgctttactt aatagctacc cgtggtgcta gttaatttta
    46921 atatcctaaa gaatat
//
LOCUS       DACIRY010000006        43059 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.006, whole genome shotgun sequence.
ACCESSION   DACIRY010000006 DACIRY010000000
VERSION     DACIRY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.006"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(125..895)
                     /locus_tag="IYE22_000260"
     CDS             complement(125..895)
                     /locus_tag="IYE22_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9505505.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(888..1823)
                     /locus_tag="IYE22_000261"
     CDS             complement(888..1823)
                     /locus_tag="IYE22_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505506.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2417..4066)
                     /locus_tag="IYE22_000262"
     CDS             complement(2417..4066)
                     /locus_tag="IYE22_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ9505507.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(4261..5157)
                     /locus_tag="IYE22_000263"
     CDS             complement(4261..5157)
                     /locus_tag="IYE22_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9505508.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(5150..5830)
                     /locus_tag="IYE22_000264"
     CDS             complement(5150..5830)
                     /locus_tag="IYE22_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9505509.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(5831..6613)
                     /locus_tag="IYE22_000265"
     CDS             complement(5831..6613)
                     /locus_tag="IYE22_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9505510.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(6606..7523)
                     /locus_tag="IYE22_000266"
     CDS             complement(6606..7523)
                     /locus_tag="IYE22_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9505511.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(7526..7810)
                     /locus_tag="IYE22_000267"
     CDS             complement(7526..7810)
                     /locus_tag="IYE22_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9505512.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            8598..11825
                     /gene="fss3"
                     /locus_tag="IYE22_000268"
     CDS             8598..11825
                     /gene="fss3"
                     /locus_tag="IYE22_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ9505513.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            11937..12251
                     /locus_tag="IYE22_000269"
     CDS             11937..12251
                     /locus_tag="IYE22_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ9505514.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            12264..12638
                     /locus_tag="IYE22_000270"
     CDS             12264..12638
                     /locus_tag="IYE22_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ9505515.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            12648..12992
                     /locus_tag="IYE22_000271"
     CDS             12648..12992
                     /locus_tag="IYE22_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ9505516.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            13061..14077
                     /locus_tag="IYE22_000272"
     CDS             13061..14077
                     /locus_tag="IYE22_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ9505517.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            14671..16545
                     /gene="ltrA"
                     /locus_tag="IYE22_000273"
     CDS             14671..16545
                     /gene="ltrA"
                     /locus_tag="IYE22_000273"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ9505518.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            16671..17027
                     /locus_tag="IYE22_000274"
     CDS             16671..17027
                     /locus_tag="IYE22_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ9505519.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            17072..18214
                     /gene="dcm"
                     /locus_tag="IYE22_000275"
     CDS             17072..18214
                     /gene="dcm"
                     /locus_tag="IYE22_000275"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ9505520.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            18204..18493
                     /locus_tag="IYE22_000276"
                     /pseudo
     CDS             18204..18493
                     /locus_tag="IYE22_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18749..19933
                     /locus_tag="IYE22_000277"
     CDS             18749..19933
                     /locus_tag="IYE22_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ9505521.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            19948..20043
                     /locus_tag="IYE22_000278"
     CDS             19948..20043
                     /locus_tag="IYE22_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ9505522.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            20813..22723
                     /gene="ltrA"
                     /locus_tag="IYE22_000279"
     CDS             20813..22723
                     /gene="ltrA"
                     /locus_tag="IYE22_000279"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ9505523.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            22827..23051
                     /locus_tag="IYE22_000280"
     CDS             22827..23051
                     /locus_tag="IYE22_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505524.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            23064..23567
                     /locus_tag="IYE22_000281"
     CDS             23064..23567
                     /locus_tag="IYE22_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ9505525.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            23694..24731
                     /locus_tag="IYE22_000282"
     CDS             23694..24731
                     /locus_tag="IYE22_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505526.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            24746..26443
                     /locus_tag="IYE22_000283"
     CDS             24746..26443
                     /locus_tag="IYE22_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAQ9505527.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            26436..26897
                     /locus_tag="IYE22_000284"
     CDS             26436..26897
                     /locus_tag="IYE22_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505528.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            26910..27770
                     /locus_tag="IYE22_000285"
     CDS             26910..27770
                     /locus_tag="IYE22_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505529.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            27808..28197
                     /locus_tag="IYE22_000286"
     CDS             27808..28197
                     /locus_tag="IYE22_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9505530.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            28184..30631
                     /locus_tag="IYE22_000287"
     CDS             28184..30631
                     /locus_tag="IYE22_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ9505531.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            30636..32744
                     /locus_tag="IYE22_000288"
     CDS             30636..32744
                     /locus_tag="IYE22_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ9505532.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            32741..33745
                     /locus_tag="IYE22_000289"
     CDS             32741..33745
                     /locus_tag="IYE22_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ9505533.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            33764..34675
                     /locus_tag="IYE22_000290"
     CDS             33764..34675
                     /locus_tag="IYE22_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9505534.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            34862..35149
                     /locus_tag="IYE22_000291"
                     /pseudo
     CDS             34862..35149
                     /locus_tag="IYE22_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(35337..35552)
                     /locus_tag="IYE22_000292"
     CDS             complement(35337..35552)
                     /locus_tag="IYE22_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505535.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(35811..35978)
                     /locus_tag="IYE22_000293"
     CDS             complement(35811..35978)
                     /locus_tag="IYE22_000293"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9505536.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(36323..37762)
                     /locus_tag="IYE22_000294"
                     /pseudo
     CDS             complement(36323..37762)
                     /locus_tag="IYE22_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(38310..38981)
                     /locus_tag="IYE22_000295"
     CDS             complement(38310..38981)
                     /locus_tag="IYE22_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ9505537.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(39330..40781)
                     /locus_tag="IYE22_000296"
     CDS             complement(39330..40781)
                     /locus_tag="IYE22_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ9505538.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(40893..42236)
                     /locus_tag="IYE22_000297"
     CDS             complement(40893..42236)
                     /locus_tag="IYE22_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ9505539.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(42370..>43059)
                     /locus_tag="IYE22_000298"
     CDS             complement(42370..>43059)
                     /locus_tag="IYE22_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9505540.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT        14016 a         7691 c         8358 g        12994 t
ORIGIN      
        1 cactaaacgg tgttcaatca gctgaccctc ggtattcgct caaaaaacaa caaaatatga
       61 ggaactattt tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
      121 ttttctaact tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa
      181 caacgatgaa taacacaagt acgatcatcg atgttgctac tggcacatcg atcgttccaa
      241 ggaatccgta acggaaaccg gagatcatat aaacgatcgg attgactttt gaaacagctt
      301 gccaaaaggg cggtaacata gagatggcat agaaaacgcc acctagataa gttaacggct
      361 gcaagacaaa tgtaggcacg atcgatacat catcataaga ctgagcaaaa atcccgttga
      421 gcagtcctgc taatgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc
      481 atgaatacac atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga
      541 gaatactgcg acccacacca ccaatcacaa agccccaaat aatcacatgc gtaggaacag
      601 gtgctaccaa gatttcttcg atatttttct gaaatttttg agagaagaag gacgaagaaa
      661 cattggcata cgaactagtg atagcagaca tcataatcaa accaggaacg atgaattcca
      721 tatatgaaaa accacccatg tcaccgatcc gcccaccgat catttttccg aaaataacaa
      781 aatacaagga tgtcgtgatc actggcggaa ccagtgtttg gacccatata cgcaaatagc
      841 ggttcgtttc cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc
      901 tcctccgtat gattattttc ttctgtgatt ttcaagaaca gttcttctaa acgattcgac
      961 ttattcctca tagaaagaac tttgattcct tgtcttgtaa gctgatcgaa gattccgttc
     1021 accccttgat ttcgttcaac ttcgacagcg attgtctgct catcttcaaa tgtacaagca
     1081 tatccttcaa tcacaggttt ggtatcatat ggcgctaaat caaagataaa tgtttcaaat
     1141 tgcagttttg acagaagctc tttcatactt gtattctcga tcagttcacc cgattggatg
     1201 attccgatgt tgcgacaaag catttcagct tcttctagat aatgcgtcgt caaaatgatc
     1261 gttgtaccat tttcgttcaa ttctcgtaga aactcccaca tctcgcgtct caactcgatg
     1321 tcaactcctg ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta
     1381 gcaatcatca gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt
     1441 tcccaaagat tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag
     1501 acaccgtagt agcctgcttg attcacgacg atttgctgga ccgtttcaaa agggttaaaa
     1561 ttgaactctt gtggaaccaa tccaatctgt tgctttgctc gaactagatc cttgtccaga
     1621 tcataaccga agacattcac ttttccagat gttttattga ctaaagaagt aataatcccg
     1681 atcgttgtag acttcccagc tccatttggc cctaatagcg catagaaatc tccttctttc
     1741 accgaaagat cgattccccg caatgcttcg accccacttc catagatctt tttcaaatta
     1801 tttatttcaa gcgcatttgc catttatttc ctatctcctt atagacatga ttcttaattg
     1861 tatcatggaa tacagcagat gaaaaaacaa cttggcttcc acacaaaaac acacctgcta
     1921 acaaagagga gatgtgtttt ctagttcatg atcatcttcg atagaataac tatctaaaca
     1981 actctggaac ttttaaaatc ataaaagtac cgacagcaac tactacgatg acatatagaa
     2041 gatccttata actgaggttc ttgtcttttt tattcatgtt aagctttcct ccgtcctttt
     2101 atcgaaagct tttttctttt attttaaccg aaataaataa aatggaaata gttattttca
     2161 atttctgttt tcgctgtttg ttttacggag aactttgcca tggaacgtgc gtctcgtcgc
     2221 tgatgctaag ttttttcgat aaaagctgct agaaggaaga cagcgtggtt tattttgtat
     2281 cgtcttttgc taaatcttcc aagatcatct ggacagcaga ctcgttccca ttgaattctg
     2341 ccactttttt ggcatcaatg tacttctttt gataatcgtc tttttggacc cacgcatcat
     2401 gcttcgtttg ataaagttaa tcaaacggat accactgacg ctgttcatcg aacaacaaac
     2461 tttctttttg ggcctgttca actatggatt taggataatt caaaactttc agcaaggcaa
     2521 tcgcatttcg ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg attccttttc
     2581 cttctgttac ttgttcttca aagtggacat tctcacaata attctttaaa atctctgtca
     2641 attcgatatc atgtgtggcg acaaatgcta aagatggata ctccgcaagc caccgaacga
     2701 ctgaagcgga agaagcaatc cgttcgatcg tatttgttcc ttttaaaatc tcatctacga
     2761 agcagtagca gcgttctttg gaagccactt ggtccagcaa acgtttgatc gatttgattt
     2821 ctgaaacaaa ataactgtcg ccttcaaata aatcatcttc gattgccatg gaagtcaaca
     2881 catgtccagg ttgcagggta aactgttctg caatcgctgt ttggatcgtc tgtgctagaa
     2941 tacagctgat ggctatactt ttgacatacg tagattttcc tgatgcattg gaacccgtga
     3001 ctaatgtatt cttctgccag ttgactggat taactaccgc ttcttttaac aatggatgat
     3061 agatatcttt cgctgtcaca ccgccctcag taaactctgg tcggcaagtg atcggcatat
     3121 aggtgcgata attcaaaacg gctgctgcaa cttccaattc tcccagcaat tcccacagct
     3181 tgatggcttc agttgaatgc ttttctaatt tagtgagaac gaaatgatag gagataaatg
     3241 gcagcatgaa catgatgttg acatattcaa aaatcaattc tgcttcactc ccgctcttca
     3301 ttcgaaatga aaaacctaat ttagggatgt ctcttaatgg tttagtttgc tgtttcagct
     3361 ctttttgaag tggtgtcgtc atttttgcta actgattggc ggctgaaatc gttgaaacca
     3421 aataactcat gctattcagc tctgtttcta gtgtttgttt cttcactaga taatagaccg
     3481 tattgaatac taagccaccg attgctagat agattcctag tacttgccct aaaagaagca
     3541 aacctacacc caccagcgga agcaagccaa gaaaaataaa taaacctacg cgtccaatct
     3601 ttttactttt tccatccgct agatattgtt ttgcaaaatt atgatctttt tttcctaaac
     3661 gagcgaattg atattggatt ctttctctca gttgaggatt ttcctgataa aaagcaatta
     3721 gtttttctaa ctgataatct tctccaaaat caaagctacg taaacgctga tacaacgctt
     3781 ctgaacctac actagaatag gtactgttga tcccttcaaa tacttcgaac atatccaagt
     3841 cataccaagt aagatcgtcg atttcactat cccatgaacg gaattttttc tccgtgagcc
     3901 acgcatcctt caaactttct tctttatcaa atctcggttg ataaggaatt tttccccaat
     3961 tactacgaac tgtttccttt actttcaaac gtgaaaaaat gtctaaaaca actaataaaa
     4021 taacaactgc aataataatt aaaacgataa attctctttg gcccatatcc ctctccgttc
     4081 cttgatttgt tagttttagt atagcactgt tgcgctattt gaaaaggggg tacaaaaata
     4141 actgtcttcg ttacctaatt cgtatcaatc acaccaaaat aaaaacacct tccaaaaaag
     4201 aagatgtctt taaaacgaga tacaattttt tgtcattcac ttcaggtaaa ttagagccaa
     4261 ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat
     4321 ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc
     4381 cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt
     4441 ttgcgctcct ggtttgatag gattggccac tgttaatcga atgaatgaat cctcttttga
     4501 aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag
     4561 cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa
     4621 cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac
     4681 ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc
     4741 aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt
     4801 taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt
     4861 aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt
     4921 ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaccc atgtattcag
     4981 tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata
     5041 ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag
     5101 gaagaaaata aaacttccca taaagataac aacgagaagc aacagccact taatcaaatc
     5161 taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct agtttccctc
     5221 gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa
     5281 ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca
     5341 gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg
     5401 actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga
     5461 cagaagtctg atgacgaagt tggatgccga ttcgtaacaa cagttcttcg atatcaaatg
     5521 gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc
     5581 tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg
     5641 cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc
     5701 ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa
     5761 gaacctcctc cagtaaagaa tgaacagctt catcgtcttc gataatcaaa atagtttcca
     5821 gcaggaacac ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata
     5881 ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt
     5941 gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc
     6001 ctttgatttt tgcgaaaaca ccgggaacga aggccgttcc catgatataa agaaaagaaa
     6061 gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg
     6121 tcgctaatat cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt
     6181 tgattccctc aacttcacca aatccgaaag caagtgtgta agcaatcatg gctagccccg
     6241 taatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat
     6301 aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt
     6361 ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt
     6421 ccaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat
     6481 tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca
     6541 tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta
     6601 atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc
     6661 ccaactaggt ggataccatc cacaggaact tttgcctcta ccaatgcttg attgatggct
     6721 gctacttggt caattccttc aaaaagctgg ataacacggc tagattggac aaaataatcc
     6781 ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt
     6841 ataaattgac gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct
     6901 tcatgcaaaa taccgtagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa
     6961 agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc
     7021 atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg
     7081 atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc
     7141 tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac
     7201 acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga
     7261 gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca
     7321 aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta
     7381 gacttacccg ctccattctc cccaatcaaa ccatagatca ttccacgttt gatttcgatc
     7441 gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag
     7501 accgtttcat ttccaaaatc catacttatc cttctttctt attgataagt gaagtatagt
     7561 tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa
     7621 tttcaagaaa aatcagactg tctcgcagac gtggttgagt tgtctgccaa tcaaagagcg
     7681 caccaaacag tgctaatcca agtggactgg ccaccatggt aatcgttgtt atcgttgaca
     7741 tcagacgtcc ttgtatatgc gttggaactt ctcgctgaag ccagaccata taaaaagcaa
     7801 aagaaaacat taatccttaa aaacggactt atgaaaaata atacatgccg tttgggattt
     7861 acaattagct accaaataag aggttatgac aaaagacttt tcacaatccc ttcttccgaa
     7921 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatttatc aaacatgaga
     7981 agcaattttt gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcag
     8041 aagcggataa ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg
     8101 aatgatatta tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac
     8161 tttccaaatt gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc
     8221 ttcgcccagt acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg
     8281 agaactgtga agctttacgg gctttcttaa gacctggttt tttcttcgta tcattgaata
     8341 agtttgaaaa gccttgattt ataaaggttt ttccgtaaat atattttgtt atatttgatt
     8401 aaatttgaaa tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt
     8461 ttatattaaa tttttaggct cattcattct caaatccatt ctatttagtc aataaatatt
     8521 ttaaaaagac tttataaaca aagatgttca agcgagcatc ttattttttt gtgcctaatt
     8581 tgaaaggaga acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt
     8641 tgagtttggc tgttcaatca attgtaggag caattgctcc tacgattgct tttgctgatg
     8701 aaattaccca tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg
     8761 aaggaacttt tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt
     8821 acaacggcgt taaacaagat attttctgtg ttgaaccagg agtcccgatt tacaatgagt
     8881 tcactcctgg atatgagaag aacccgttac cagatatgtc cgataaagcc aaattagttt
     8941 ccgttttatg gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga
     9001 tctggcaaga agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg
     9061 taaatatcgc agccattgag gcaaaaatca atcaagccat tgccgattat cagaagaaac
     9121 caagcttcca taatagtacg gctaaaacag tattagggca atcgactact gtgacggata
     9181 cgaataatct gaatttgtca gagttcgatg aggttgtgga aaataccgca aacattgatt
     9241 atcgtgtgaa tggcaatcag ttgataatca gcccaaatgc caattcaaaa gaaagtggtg
     9301 tgttgacgct taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc
     9361 aacagacttt aatggccggg gcgatcgata agccgaacac ttatacggtc aaaattgatg
     9421 tggaaatgga gggttctttg aagattaaaa aagttgataa agaatcgggt gatattatac
     9481 caggaacggt tttccattta gactttggaa aaactttacc cgcaaaagac gtgaccaccg
     9541 ataaagaagg cattgcgacc ttagatgaga ttcctcatgg tacaaaggta accattactg
     9601 aaaaatcggt gccggcgcct tatacgattg ataccacacc catgactgcc acaattaaag
     9661 caggtgaaac catttcagtg acttcaaaaa atacgcgaga aaaaggtcaa attattctgg
     9721 ataaaaccgg aattgaaaca gggagtgatc tttggaacga taattactct ttagcgggca
     9781 atacgtttgc cattcgcaag gatagcccaa ctggtgaaat cgtccaagaa atgaccacgg
     9841 atgaaaaagg tcatgcagaa acaccaaaag agattgccaa tgcgttggaa ttgggaacct
     9901 actatgtgac tgaaaccaag gctagtcatg gctttgtgaa tactttcaaa ccagtaaaag
     9961 tcgaattgaa ttatgtcaat caaacagtgg ctcttgttac cagtaacgta aaagggcaaa
    10021 accaagaaat tactggtgaa accactttga caaaagaaga caaagatacc ggtcatgaaa
    10081 ctcaagggaa agctgagttt aaaagagctg aatacacctt gtttactgtt aaggatggaa
    10141 aagcggtcaa atggaccgag aactttaaac cagaattgtt gaagggaacg aaagcttctg
    10201 atgaaaccgt aactttgact ttagatgaaa agaatcaagt tgccgttaaa cacttagcga
    10261 ttaatgagta ttactggcaa gaaaccaaag cgcctgaagg atataccttg gatgaaacaa
    10321 agtatcctgt atccatcaaa aaggtggatg atagcgaaaa aaatgctgtg attactcgag
    10381 atgttgcggc aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat
    10441 caactactgg cactgccgaa actggcttca atgatctaac ctttaaagtg tcgccattgg
    10501 aaggaaccaa tgaaatcacc ggtgccgaag atgaagcgac cacagcttat aacgagcaat
    10561 tagggtttga tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag
    10621 aagtagaagc cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta
    10681 aagaaaataa agaggattat accaagagtg attatatctt tactattacc gaaaaagggc
    10741 aaaagcaacc gattaaaacg gtaacggtcc cttatgggaa tctaaccaat gagaatttct
    10801 cagtcagttt gaaccgtttg atgctttatg atttgcctga ggaagaagat agcttaactt
    10861 ctcttgcgac ttggaaagaa ggagataaag aattgactac tcttgattct accgagctag
    10921 tcgataaatt gagctataat ctacatgaaa tcaaagaaga ctggtatatc gtagctcaag
    10981 ccatcgatgt agatgctaca aaagctgccc aagaaaaaga taaaaaagcc aaaccggtgg
    11041 cgattgcgga aacaaccgca actttagcga ataaagagaa aacaggaact tgggagattc
    11101 tgcataaatt aactgtcgaa caagttttag acaagaccat tgtcttattc aactatgtgt
    11161 atgaaagcaa agaagcctat gaagctggtg atgagccggt agcgaaggat gctagcttga
    11221 acaatcaagc gcaaactgtt aattgtatgg tggaacgtca tgtttccatc caaaccaaag
    11281 cgcacttaga agaaggttct cagaccttta ctcatggtga cgtggtagat atgtttgatg
    11341 atgtatccat tacccatgat gtactagatg gctcaaaaga agcttttgaa acaattctgt
    11401 acgctttatt tccagatggt acaagtaaag aaatttggaa atctggcaag attgattacg
    11461 aagtgaatga caaagaattt accaaaaccg tacttgcgga aaaagtggat acaagtaaat
    11521 atccagaagg tacaagtttt accttcaaag aaattaacta tgacaaagat gggaatatca
    11581 atggaaaaca caatgaagat ttgaaagaaa aatctcaaac tttaacgccc aaaaaagttc
    11641 cgacaacacc aagtacacca gaacaatctg aaatgccaac tgttccaagt ggctctcaag
    11701 aatctagtcc tacagtgaag gcattccctc aaaccgggga gaaaacttcc aatgtgctac
    11761 tgttcattgg ctttagcttg atctttgcga cggctggcta ttatttttgg aatcgtcgga
    11821 actaaggtga taagatgaaa catgtaaata gtagaaaaca atacgcgcga ccgcctccta
    11881 aaaaagtggt cgcgctattt ttgtattcaa aactaacaat gaaaagagga aaaaatatgg
    11941 aattgaaatt tgttgtacca gatatggtcg agacgttcgg aaaactaaca tacggtggtg
    12001 aaggtgaagt tctatcggaa gggtatggtc gaaatactac aattgttggt cgaagttacc
    12061 atctgttttc aaataaacaa cgggccgatg atattgaagt ggtggtagct gcggaagccg
    12121 gcgaaaagga ttttgaccaa gatcaaccac taaaagctgt aaatccccac ttaattgcca
    12181 aaggctatga aattgagaat cgtgggttta ccgattacgt gttgtatgtt gatgatttag
    12241 tgaaagcata ggaggaaaag aagatgagat taacagaagg tattgtcgta gattcaggat
    12301 taacctttgg caagttgcgt ttttccgcat tacgtcgaga agtacgcaag caaaatgagg
    12361 acggaacggt tagtaacgag gtaaaagaac ggacctataa tttgaaatcc tccgcgcaag
    12421 gtcgaatgat ccaagtcagt attccagcaa atgtaccctt acgcgagttt gcttatgatg
    12481 cagaggtaga gctggtcaat ccaatcgtgg atacggtcgc caattatgtt tttcgggaag
    12541 gaaccaccgt taattggttt atcaaggcag atgatttagt cttgaaacga caactgaatc
    12601 aaggaaaacc tacgaaccaa aatgaaggaa agaaataggt gaagaatatg gaaattaatg
    12661 ttgaacatat tttagattgt ctcgatcaat ttggcaaagg tgagctaacg gaggaacaac
    12721 tcactaacgc attaacctat gatgaaaaaa tgtttctgat tatgcatcaa gggttactgg
    12781 aagtaggtaa cgatgccaaa gaagactttg atgtactgga ttggttaggg gaagaagagt
    12841 ctttctttat ggttgtcgag gtaaatgaaa gtttatgtcg gcaggcagaa tctgttttgg
    12901 aagaaatcga tgtggagatg ccggatgcca tcgaaagatt tttaaaacaa ttagtggaaa
    12961 cgaaacaact tcccgtagta gtaaatgact agcataactt tttgaaattg cccaatgaaa
    13021 tcctattggg ctttttcgac tggaggtgga aaccatcaaa atgtacaaag gacatcgcat
    13081 acgcgcagga gatcaacact tgatttatca ctttgttctt ggctggctaa tctttttgtt
    13141 cattggttgg atgagcgtct tttatttaca agaacttaga caatttgata tttctaaggt
    13201 atcattttcc actagtgaaa ttggctggtc tatgaaggat gtaatctgct tgctaggaag
    13261 tctcgctctt tcaggaagca tgatgttact ttatatacac ttttttcagg atcattggcg
    13321 aagtctttgg catcgacaaa agttagctcg gatgattcta gaaaaccatt ggtatgaagt
    13381 aaagcagact caaggtgaag gttttttcaa agatttgaat agtagttgga ccaaagagag
    13441 tatcagttac ttccccaaaa tttattatcg aatgaaagat ggcttacttt ctattcgcgt
    13501 tcaaatttca ctgggaaaat atcaggatca gctattgaag ttggaaaata agttagaaag
    13561 cggactgtat tgtgagttag tagaaaaaga actcaaagac tcctatgtgg aatacacctt
    13621 gttatacgat atgattgcca atcgaattgg gatagaagaa atagtggcag aaaatggggc
    13681 gttgcgattg atgaaaaatc aagtatgggc gtatgattcc ttgccgcata tgttaattgc
    13741 tggcggcaca ggtggtggga agacctattt cttactcacc attattcaag cactattgaa
    13801 gtcagatgca gaattgttta tcctcgatcc caaaaatgcg gacctcgccg atttaggtac
    13861 gataatgcct cacgtatact cccaaaaaga agaaatttct gaatgtgtgg aagattttta
    13921 cgaacgcatg atagctcgta gtaggtcaat gaaagaaatg cccaactaca aaacaggaga
    13981 gaattatgcg tatctcggac ttccgcccaa ctttttaatc tttgatgaat acgtggctta
    14041 tatgggtgcg aatcgttttc ctacaagcat cgaatagatg tgaaatggaa gcctattgag
    14101 attatcaata gtgacaacta atgttttgag agatggaatg aaagccttca agttgagctg
    14161 aaacatcccc gctaatactc ctacatgctc taatcaatga agtagattgg ttttggtatg
    14221 aagctaggtg aagtcgtctg aaatctgcct aagtgcttga gcataaggct gactataggg
    14281 cgggcggctg aaaaagataa ctatggtaag aatgtctgaa aatgactgac gaatctgcga
    14341 atgtacgggt ctaaaaggct gatcttattg aaagatagga acatacggac gtatggtgag
    14401 ataccgacaa gtaaggttcc atgatatgaa aatcgggata gctaacgaag aagctataat
    14461 ttcacaggct catagtagat acctaaggtt atacatgata gctaaaaaca ttggaacgat
    14521 gtaagcagaa aactatcaat taaatgacta cttctagata ggtgcatata agtaataacg
    14581 aaattgcttt gtttctgtga aagtaagggc acagtaccga tgaagcgtct aacaaatgtg
    14641 gagggatagc cctaagtcta tttaataaca atgaatgttg aaacagatga agttcaagga
    14701 tctggtaaga acgtgggaaa atccgttatg gaggtgccac gcttgaacac aaaaatacgt
    14761 tactgggaat attatgatct acaagaaaca ttcgatcagc tatttactca aagtagaaac
    14821 gggaaaaagt tttatcaatt atatgagtta atcatttctg aaaataatat tcttttagca
    14881 taccgtacta tcaaggcaaa caaggggtct tcgacaccag gaacggattc cttcacaatt
    14941 gataactata aggaaatgaa ccaagcagag tttatccatc ttattctcag tcatttagaa
    15001 aattacaaac caaaatccat aaagcgagta atgataccta aacccaatgg tgagaaacgc
    15061 ccattaggaa ttccatgcat gattgatcga atgatccaac agatgttcaa gcagatactg
    15121 gagccaatat gtgaagctaa gttttatgaa catagctacg gtttcagacc attgagaagt
    15181 gcaaagcatg ctctaggtcg cattatgtat ctcataaaca tcagtaaaat gcactatgca
    15241 gtagacattg atatcaaagg tttctttgat aatgtaaacc accgtttact gataaaacaa
    15301 ttatggaata taggaatttg tgacaaacaa gtcttagcta ttctttcaaa atctctgaaa
    15361 tcacccattc aaggagaagg tatcccgagc aaaggaacga tccaaggagg tatcatttct
    15421 ccactattat ctaatgttgt tctaaacgac ttagaccatt gggtctcaaa gcagtggcat
    15481 acctttgaaa caaagtatcc ctatactaaa ggttacaata aatttcgtgc tctaagagat
    15541 acaaatctaa agcaaggcta tattgtcagg tacgcagatg actttaaaat catgactaat
    15601 gattatccga cagcattaaa atggttccac gctgtcaagc tttacctaaa agacagactt
    15661 aaattagata tttccaatga gaagtctaaa atcgtcaatt tgcgtaaatg taagtcggaa
    15721 tttttaggat tcgctatttg cgtgaaacaa aaagggaaaa aatgggtttg taattcacgt
    15781 atttctaaca aaaaaaagga tcagattaaa gaagagatta aacaaagaat caaagatatt
    15841 caaaagagtc ctacagcaca aaatgctctc ctgtttaatg ctctcgtgct cggtgttcat
    15901 aattatttca ggtatgccac tcatattaat ttagatttga ataggatgaa ctacgttctt
    15961 tcattcacta cttacaaccg attatcaaat tgtagtacaa gatgttaccc caacaaagct
    16021 tccccaactt acaaaaagtt ttactcaaca acgatgaaaa cgtataaagt agctggagtc
    16081 tatttgtacc ctctttgcga cgtatctaca aaaacaattt acggatttaa tacggaggat
    16141 acaccgttta ctccgtttgg tcgtcaaaga ttggagcaca aaagcctaca gtctcttgtg
    16201 ttccaagaat taagaaaatt gatggaatcg aaaataccga atagaacggt tgagtattta
    16261 gatagtagaa tatctcgtta ctcaatgaaa tcaggcaagt gtgagattac aaaacaattt
    16321 ttgccagcaa aagccgttca ctgtcatcac tatttgccaa aaagtcttgg tggagacgac
    16381 aaattcgata acttgagaat tattcataag gatatccatc ttctgatcca tacaacaaat
    16441 aaaatgataa tcgatcacta cgtgcatgaa ctgaaactgg tgccagagca aatagcaaaa
    16501 atcaaccttt atcgaaagat gtgtaatctg caaaacattc aatagaaaat aaatagatgg
    16561 aacgccgtat gaggtgaaag tctcacgtac ggtgtggagc gggggaaaag atggagatta
    16621 tatcaaattc ttacctatcg ctatagatgt taacgacaaa agaaagtgcg gtgattttga
    16681 ataagcttaa acaaatcgtg atgttaggcc gtcaatctgg tttctttctg attctagctt
    16741 gtcaaaggcc ggatgcgaaa tatctaggag atgggattcg cgatcagttt aacttccgtg
    16801 tggctttggg tcgtatgagt gagcttgggt attccatgat gtttggagaa gtcgataaaa
    16861 atttttttat gaaacgcatc aaaggacgag gttatgtaga tacagggggc agtgtgatta
    16921 gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttaagggaa attagcaaca
    16981 ttgttggatt aaccgttcat acggaaagag aaaataatag tgtgtagcct aggaatatca
    17041 ataatcctgg gcttttttat tgaggtgatt tatgacattt ttagatttat tcgccgggat
    17101 tggtggcttt cgcttaggta tggaacaagc cggtcaccag tgtatcggtt tttgtgaaat
    17161 tgatgaattt gctcggagga gttataaagc aatccatgat acaaggaagg aggtggaaat
    17221 gcatgacatc acaagtgtat cagacgaatt tgttcgatcc ctcggaccag tggacatcct
    17281 ttgcggcggc tttccgtgcc aagctttttc aattgcggga aagcggcaag gattttccga
    17341 tactcgaggt actttattct ttgaaatcgc tcggttcgcc actcttctcc aacctaagtt
    17401 tctattcctt gagaacgtcc ggggattgct caatcacgaa ggaggggcta cgttcgagac
    17461 gatcctccga acgttggatg gattggggta tgatgtggaa tggcaagtgc ttaactcaaa
    17521 ggcctacgtt ccccaaagcc gtgagcggat cttccttatc ggacattctc gagatgcatg
    17581 taccgaacaa gtatttccta tcattggatc gtcttcaaca tctgatcaaa acatcagaaa
    17641 cttggtaaat atcaatcctt ccaatcgagg aatgggtggc caagtctatg gttcagatag
    17701 cgtagcacca accttgaccg gtgatgaagg aattaaaatt gccttaccgg taaaggatgg
    17761 gatttctgta gcgggtatgt tgtcgggaaa ttttgaacaa ggcaatagga tttatgaggt
    17821 aaccggaaca gcccccactt tgtcaacgaa acaaggggga acgaagatta tgattcggaa
    17881 aaatgaaaca gaatatcaag aggtgaagcc aggggacagt gtgaacctgg cttttcctaa
    17941 ttccacaagt cgccgaggtc gtctgggaaa acaaagtgtg cataccttac taacaggaga
    18001 ccaacaagcg gtagtcacgg atcagtacca gattcgaaag ttaacaccta gggagtgctg
    18061 gcggcttcag ggctttccag attgggcttt tgatcgagct tctcaagtca attcagatag
    18121 tcagttgtat aaacaggccg gaaactcagt aacagttccg gtcatttttg atattgctag
    18181 aagattaaag ggggtaaaag catatgattt atgacgagtt gattggagaa atctactgga
    18241 tgattggcaa gattcaggca gatccgaaat tggaagaaga actgtaccgg ctcaattttg
    18301 atattcgcaa aaacggggtg aaagtccagg tgattcgtat gtagtggatg aggaaacgga
    18361 tgctcgaatg gagctcaatc aagtcatcgg tgaattggaa cgaattgcgg accttgccaa
    18421 agaacctgat atccgccaat acttgtttga gataaaagca gagttggaaa ttgagggtat
    18481 caccgcggag taatagggaa ataacccact gataggcact gaccgcgggg gatttgcgaa
    18541 gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc ggcagccggc catgatgtct
    18601 caccccccgt atctaacagg ggggtacaaa aacacaaaaa acaagcaagt aaccataaaa
    18661 aatgcttgtt gtatcaagga tattaagtaa tttgtatagg tgttaactca tttggaaaag
    18721 ttaacacctt gaatttggag gtgaaaactt ggcgcaacga aatttagatt atcggttatt
    18781 aaaagaacga cgcaacgaat atggcgtttc acaaaataaa ttagctactg cttgcggact
    18841 tagccgccca tacctaaatc agatagaaaa cggtggcgta atcgcctcta caaagaccat
    18901 gagaaaaatt tttgatcaac tggaaagttt caatcccgat ttacctttaa cgttactatt
    18961 tgactatgta aggattcgct ttcccacaac ggatgtgcgg aaaattattc aagaaattct
    19021 ccatttgaaa ttcgagtata tgcttcatga agattacgcc ttttactcct atcaagaaca
    19081 atatgtcatg ggcgatattg tagtgatgtt atctcatgaa gaagataaag gtgtcctttt
    19141 ggaattaaaa ggtcgtggtt gccggcagtt tgaaactttt ttactcgccc aaaagcgtag
    19201 ctggtacgac ttttttgaag attgtctaaa agccggtggc gtgatgaaac gcttggattt
    19261 agcaatcaat gatcgagtgg ggctactgga tattcctgat ttaacgaaaa aatgtcagaa
    19321 ggaagaatgt atctccttgt ttcgtacctt caaaagttac cgttccgggg aactgttgaa
    19381 agctgatgaa aaggatggta tgggcaatac cttatatatt ggcagcctca aaagcgaggt
    19441 ttatttttgt ttgtacgaaa aagattatga acaatatatc aagttaggga ttccattgga
    19501 ccagaccgaa acgaaaaatc gttttgagat tcggttgaaa aatgaccgtg cttattatgc
    19561 gattcaagat ttgttaaaag gtcgtagcat tgaaagtacc acgttttcca ttatcaatcg
    19621 gtatttgcga tttgcggata aagtagatgg aaaacggcga accaattggc ccttgaatga
    19681 acaatggggt cgttttattg gacggaatcg gaaagagatt caattaactt ctgaaccgaa
    19741 accgtatacc attgaacgaa cattaaattg gctaggtaga caagtcgccc ctacgtggaa
    19801 aatggctaag gagttggatc gattgaatca aacaacgtat atccaggata tggtacagaa
    19861 tgcgcgactc tcggaccggc ataaaaagat tttggagcaa caaagtatgg caatcgaaaa
    19921 cttgattgta tgaaaagagg aattttgatg gaaattgtaa aagatatgtt attggtactt
    19981 ggtggcagta tgcttggtgt taccgtgatg tgcttgatga atgcaagtgc agcagcggat
    20041 tgagcaatgg aaaagaaagg aaagtgacaa aatgaatttc ggacaaaatt tatgtgcgac
    20101 tcgttcctta gtgaaaagct aaggcactaa taaaagaacg aaaaagtttg gttattagga
    20161 gaactgataa tctgacaggt ggaatgatga agtaacgctc tgaaacgcct gccctgagcc
    20221 tccgacaagc attgtatgag gaaactcaga atgtttgaag ctcggtaaag acggctgaaa
    20281 gataccgtaa tagttgatga acatatatct tctatcgaaa actatccaga ggtgggtagc
    20341 gtatccaaca ggtcgggggt ctataaaata tctatggtta gaatgttcta taaggaactg
    20401 acgaaacagc gaatgtacgg gtctaaattt gaactttgta gaaatgcaaa ggtacgagaa
    20461 atcgtagctg gtgtggcaaa gtaagaatat aggttatgaa atgccatata acgttacagg
    20521 cggtatcaag ccagcaggct tatagctagt gacctaagga tatatatgta tagataagat
    20581 tatcggaacg aggaaaggta ttgggttctc atcaagagaa taaactgacg aagaacaata
    20641 agcagttgaa ctaatgctga aaagcagagg gcgaacctaa gaaattcttg taatgagaat
    20701 ggaggaatgc cctcaagtcg tgtatatatt agaaaagtat tatgttgttt caataaggat
    20761 tacgagtaag ataaaaaagc tcactcaatt tcaagtaaag gagtgaagcc ttatgcctaa
    20821 aaataaaaat gaaacattgt gtgtagaaga tttaagacac gctgaatatt acgaaatgca
    20881 aaacaccttt gatgatttat atgccaaaag taagaatgga gatattttca ctcatcttat
    20941 ggatattatc ctatcgagag aaaatatatt actggcatat cgaaatatca aagcaaatgc
    21001 aggcagtaaa acagcaggaa cagacggtac aataattaaa gatattggca aattgccagc
    21061 agaaacagtt gtcaaaaaag taagatacat tgttgcaggc agtccacatg ggtatcgacc
    21121 taagccagtg aggcgaaaag aaataccaaa accaaatggt aaaaccagac ctctaggtat
    21181 tccttgtatg tgggacaggc ttatacaaca atgtataaaa caagtattag aaccaatctg
    21241 cgaagctaaa tttagtgaaa acagctatgg ttttagacct aataggtcag ttgaaaatgc
    21301 aataaaagca acctataacc gcctacaaat cagccagcta cattatgtga ttgaatttga
    21361 tattaagggt ttctttgaca acgtgaacca ttccaagctg ataaaacaga tatgggctat
    21421 gggaatacgg gacaagcact tgatattcat tttaaaaagg atattaaaag ctccaatcaa
    21481 aatgactaac ggtacaataa catatccaga gaaaggaact ccgcaagggg gaataatatc
    21541 cccactacta gcaaacattg ttcttaatga gttagaccat tgggtagaaa gccaatggca
    21601 ggaaaatcct gttacaaaaa actatgtggt tcatatcaat aaaagcggta gcccatgtaa
    21661 aagcaacgct tacaaagaaa tgaagaaaac aaagttaaag gaaatgtata tggttcgtta
    21721 tgcagatgat tttagagttt tctgccgcta caaggaaagt gctgaaaaag ccaaaatagc
    21781 aatcactcaa tggattgaac aacgattaaa attggaagta tcacaagaaa aaacaagaat
    21841 tgtaaatgtt agaaaaagat actccgactt tcttggtttc aagattaaaa tgataccgag
    21901 aagaaagaag ttagttgtta aatcacatat aagtgataaa caattcaaaa accaaaaaga
    21961 caagctggta tcacaggcaa agaaaattgc ttctccctca aaagggaaaa cggaattaga
    22021 agaaacaaga ctttataatt caatggtatt aggtatgcaa aattattatc agattgcaac
    22081 gaatgtaaat ctggattgta tgaaactgaa tagagctgta atggctatct tcactaacag
    22141 attaggtaca aacaaaaggg gcagattgag aaaaacaggc agacaactta cgacaaagga
    22201 ggcaacaaga tatggtaaat ctaaaatgct tcggtatgtt gctggcgagg gcgaacctat
    22261 tttccctgtt ggatatatcc aacacagaaa accaatggca aaggtgtatt tagcgaattg
    22321 ctacactcta gagggaagaa aatttatcca caccaatcta agtgttgata aattactgat
    22381 gtaccagtta atgaaattat ctttggaaaa tcgcacaata gaatacgcag acaatcgaat
    22441 ttcgctattt tctgctcaac atggaaaatg tgctataact ctggaagaat ttcaacaagc
    22501 taatgaaata cattgtcatc atattgtacc aactggagct ggtggaaatg acgattataa
    22561 aaatcttatt ttggtaaaag aggcggtaca ccgtctaata cacgctaaaa cagaagaaac
    22621 aatacaaaaa tatatagctc tattgaagct aaataaagtc cagctcataa gactaaataa
    22681 atatagagta ttagctggca atcaagaatt aaacttgata taggtctact acataaatac
    22741 ttttcaatat aaacacgatg gaacgcggag tgcggtgaaa gtcgcacgct ccgtgtgaat
    22801 ggggggaaaa gtttgagata tttataatat caggaactta cctatccata tatcaatggt
    22861 ttttaaccaa tgctcaatct ttagtcttac tagcgattgt ggtcattggt ttgtttttag
    22921 gatttaaacg ggagttttcc aaactgattg gcttcttggt gattgctttg attgcggtag
    22981 gtctggtctt taatgcttct ggtgtgaagg atgtcctact caatttgttt aaccgaattg
    23041 tcggtgcctg agggggaatc acaatggaac agatgcgtgt ttatattgcg aaccttggaa
    23101 aatacaatga aggtgagttg gtgggggcat ggtttacccc acctgtcgat tttgatgaag
    23161 tgaaagaaca gattggatta aatgacgaat atgaagagta cgcgattcat gattatgaac
    23221 tgccttttga gattgatgag tatacgccaa ttgaagagat taatcggttg tgtaacttag
    23281 cggaggaact agaaggaacg ccaattggtg aagctgcttc tgagattcaa cacgctttct
    23341 tcaattcttt cgaggaaatg gtagagcatg tggatgatat tgtctattat ccagattgcg
    23401 atgatatgag tgatgtggcg cattacttga ttgtagaagc tggtgatttt ggcgaagttc
    23461 cagaacaatt gaagccgtat attgattttg atgcttgcgg tcgagatttg gagattggag
    23521 gaaattattt ggttactagt cgtggagttt ttgagtatcc agaatgaatc aaacactata
    23581 tatagtgttt gattagtata tgtaagcaaa aaaacacaat atatagagtg caaactgaag
    23641 ggtaatgtga tataatattt gccgaagaaa tatttttgaa atgaggtaat attatggcaa
    23701 atattcaaac aagttttatt gatttccata attcaattag attggacgta gaagacaata
    23761 cgctacttaa ggactataaa gaccaagtaa ttgatggatt aaaagattac ttacctgatg
    23821 atgtgaagtt tgaaactttt ttacagggaa gctattctgt ttataccgga ataaaaagct
    23881 gtgatgaaaa aattgatttt gacatcgata ttgcagttgc tttcgaaatt gatcatactg
    23941 tttatgaaga tccaagggag cctaaactgt gggtaaaaga ggcgctagtt gaaatttttc
    24001 ctaatgctca agttaattta aaagtacctt gtgtaactgc tacatttact ggaaaaaaga
    24061 caaaaaaaaa tgtgcacgtt gatgttgcgg tttatgctaa agaagatgaa aattatttct
    24121 tagcaaaagc aaaagaattc agtgctcccg aaaaccgatg ctgggaggaa gcggatccca
    24181 aagtattaaa agagaaaatt aatagtcatg tagcagattc agatgatcga aaacaatttc
    24241 gaagatgtat tcgctattta aaaagatgga aagataacaa ttttaaccaa gaatataaac
    24301 caacaggaat tggacttaca attaatgtga tggatacgtt cctagttaat aagtccactg
    24361 attttttaac tagaaaagtt cagtataatg atatggaatg tatgaagcag attgtttctt
    24421 cgctgaaaga ttcatttgtg tatgagtaca gtgaaacaga tggttggcat tatcgtctac
    24481 atgcatatct tccagtgaag cctaacagtg acacctattc aaaaatgaca gtgaatcaga
    24541 tgagtgattt taaaaacaaa ctttctaaat tatatgatga tttgattttc gctatcgata
    24601 ctgaagatga gtatgaagct acaaaacgat taaataatca atttggggag gattttttaa
    24661 ttatttcaga agaagaagtt acagaaaaaa acttaagaaa tgcatttgtt acagactatc
    24721 cgagtgctta ggttggtgtt aaagattgga ttatactgat attttaaaag aaaagctaac
    24781 agatgttgtg cgtttattcg atggtaatta ctatggggat ttttcatttg ctacaattgg
    24841 aaagttgcgt ttaaaaattg aaataccaaa acattttcca ttggtacttc ctaggattta
    24901 cattcataat tttgatcatt taggacttta cctaccacat gtagaaaaag atgggttggt
    24961 atgtttttct tcagatataa attatttagt agatgaagaa aattcagaat tggttttatt
    25021 gcagtgtgtt aataaaacta taaaaacaat tgaggaaggt ttgaaagggt taaataaaaa
    25081 tgattttcga aaagagtttg ttgctttttg ggacaaacaa gagaatcaaa tttcgttatt
    25141 ctacctttgt gaaccagcgt tgtttatcaa gtcagttgat acaataatgt acggtaagtc
    25201 tttggttgct ttggataaag agaacgaatt acaattaaat gcagcgagta gattgtttaa
    25261 tggagagaaa gaagatactc atgaattaga tgcatactat gttccaatta gaaacaagaa
    25321 taatattatg cccccgaaga ataataaagc atggtcaaag aaggatattt tacaaatagt
    25381 aaaaaaaaat gtaacaggtg caaataaaag atttttttta aaacttgaaa aaagaaagaa
    25441 tagacataat gttttactta ttctagatat cccaattgaa gaaaagtcaa ataatgtcat
    25501 aattggattt ctttttacaa aacaatctgg atctataaaa gattataagg gtacgcctag
    25561 gactgtatta gttcagcgtc atgatgaatt atatttggtg aatagaacga gcggagaaaa
    25621 ctatggattg ggtgctaata tagctattgt tggggtaggg tcagttggca gtaaaattgc
    25681 tgaaaatctt ggtccattag gtgtgagaaa gatgacactc atagacaatg attgttttca
    25741 agctgaaaat ttgtatcgaa attcacttgg tgcagatagt ttaaaaaatg cccgatctca
    25801 gaataatttt tttaaagttg atttaatgca ggaggagctc gaaaaaaaaa tcccatacct
    25861 ttgtgttgaa gtggagtcaa tgaacgtttt agacttaatt agtatgcaac ctggtttttt
    25921 caaggaatat gactatgttt ttgtttgcat aggagataca atggcaagcc tggctttaaa
    25981 taattatttt tccaaaagta gtcaaaaagt tttctattct tggctggagc catatggcat
    26041 tgggggacac actctattta taaattattc tctaaatcaa aaaggttgtt tcaattgtct
    26101 ttatacagat tcaaactcta aaaataggtg ttcaaataga gcttctctgg taaaagaagg
    26161 acaaattttt gaaaggactt tagcgagttg ccgctcaaaa tttataccat ataatgctat
    26221 tgcctcgacg gaatgtgcaa ttaatgcaat aaaacttttc cataaagtca atcaaaataa
    26281 tatgaccaat agtactcttt tttcatggat aggagatggg agtacttttg agaatttagg
    26341 atatcaatat agcgacagaa atacttttct taatggagtc aataagtttg aagaaagatt
    26401 cttttctaaa tattgcaaag tatgtgggga gttagatgat taaattttcg tgtgaccgta
    26461 ttgtcattga agtaagggat gaagttctta agatatttga acaatatcgg cagggaaagg
    26521 atgacttgcc tgaagctgga ggagttcttc atggaaaaag acttaagaat gggagcataa
    26581 taattaccga tataacaaca cctcaaatag gagatataca aaaaagatta ctctttaaaa
    26641 aaaaccgaga aattcatcaa cagctttcta atgagaaatg gatagaatca aataagtttt
    26701 ctattgcatt gggagaatgg catactcatc ctgaagctgt accaactcca tcaaatgttg
    26761 ataagaaaag ctggagactg aacgtgagca agcaacatga tgatagagtt tatgttttta
    26821 ttatcgttgg attttctgat ttgagaatgt ggttactgtc aaaaaaatca gatattaggc
    26881 aggctataaa actataaagg agtgattagg tgaaatcaca aaagaatatt tggtggtggg
    26941 ggtttttagt aggaaaggta atgataccag cccttctctt aatttctcta ccgatgatta
    27001 cttttttctt taccaataaa aaaatgagta aagaagccgt gatttttttc tttggagacc
    27061 aaaaagctac ctttattgaa acgttagtaa catctcttca gcttaatttg aattacattt
    27121 tttcaattat tattgttata tcgttattga atttttttaa aaaaagaaat tcaggaaagg
    27181 tgtttaactc taatggaaat gtgtactaca actattttta ttttgtattt tgggtagcag
    27241 caactttact tggttatgat aaaattcaga ttgcaggaat tcctatacac atgcaatata
    27301 agttggtttt gagtggaatt ttttctgagg tgttgccaga tatatacgat gatcattacg
    27361 acagtgatgg aacatgtaaa gtaagcattg aaaaagaaaa tttcgatgac atcgatggat
    27421 atgactctgt gaacttactg attattgata cttacgatat aaaaatgagt gaattatcta
    27481 tggaaaatca aacatatcct acgataattg ttagagggaa ttcaatagat ggagttcgta
    27541 aagttaatag atctttaatc ttggaaataa aaaaaacaat ggatgaaatt caaaaaagtg
    27601 actttaaaaa agtatttgtt gcttcaacat ctaatccaaa aaatagtatc aatattatta
    27661 attcatcgtt tagatttttc ggtcgatcac gtaggtttaa actttatgtt cttcaaaagg
    27721 attatgcaag caatggaaag tatagtaaga aatatagaat ttttatttaa gaaggaattt
    27781 ttccttcttt ttattttgga ggaaactatg aaaaaaataa aaagctacac cagtatttgg
    27841 gcagttgaaa aagtcattta tgcgattaat gactttcagc tgccttttcc tgtgaccttt
    27901 aaccagatgg cttggtttgt tttatccttg atgtttgtca ttgtatttgc ccatgtgcct
    27961 ccgctatcca tgatagaggg ggctttttta aaatacttgg gcattccgat agcggtcact
    28021 tggttcatgt cgcaaaagac ctttgacggt aagaaaccat tgggattttt acgatccttt
    28081 atcagctatc acttacggtt taaggtcact tttgcaggaa agaaagttaa agaacagaag
    28141 aaacgatggg acgaaccaat caccttggta aggagtgtga attatgtacc cgattaagta
    28201 tattgaaaat aatcttgtct ttaaccaaga aggcgagtgt tttgcttact atgaattagt
    28261 gccttacaat tactcctttt tgtcacctga acagaagtat caagtacatg ataactttcg
    28321 ccaattaatt gcgcaaaatc gtgaaggtaa gatccatgcc ttacaaattg ccacagaaag
    28381 tagtattcgt gccacacaag aacgttcgaa aaaggaaatt accggtagac tcaaagaagt
    28441 agcaaagcaa cgaattgatt tacagacaga tgcattagta tccatgattg gcgatagtca
    28501 gattgattat cgcttcttta ttggctttaa actgatttcg acagatgaag aggtcaatct
    28561 aaagaatctc aggaaatctt tcttttctgg acttcaagaa tttgtctatg gagtgaatca
    28621 tcatttgatg ggtgattttg tttccttatc caatgaagaa attcgtcgct ataccaagct
    28681 tgaaaaacta atggaaagta aattggctcg aagatttaaa gtcaaacgag taacgccgag
    28741 tgacttgacg tatttaattg aacatatcta tggtgaaaaa ggcacaccgt ttgaagaata
    28801 tgaattccag ctaccaaaga aaaagctgaa atcagaaacg ctggtgaaac gttacgactt
    28861 attacgtccc actcgttgtt tgattgaaga aaagcctcgg tacttacgga tggaacatga
    28921 aaatcatgag tcttatgtag cgtacttaac aattaatacc attgtagggg aaatggagtt
    28981 tccttcctcg gaacttttct attaccagca acaacaattt acgtttccga ttgatacttc
    29041 aatgaatgtg gaaattgtga cgaataaaaa ggccctagct accgttcgta ataagaaaaa
    29101 ggaattaaaa gatttagaca accatgccta tcaatctgat aatgaaacga actccaatgt
    29161 attagatgct ttggattccg tggatgaatt ggaaaccacc ttggatcagt cgaaagaatc
    29221 catgtataaa ctaagctatg tggttcgagt gagtgctaaa tcagtggatg aattaaaacg
    29281 ccgctgtgat gaagtgctcg atttttatga cgataccaat gtaaaactag tccggccctt
    29341 tggcgatatg atgggtctac acaaagaatt cttaccctca agtaagcgat acatgaatga
    29401 ctatattcaa tatgttacgt ctgacttttt ggcgggactt ggttttgggg ctactcaaat
    29461 gctaggagaa ttagaaggca tttactttgg ctataacgta gatacggggc gcaatgttta
    29521 cctgaaaccg gcattggcat cacaaggggt caaaggctca gtaaccaatg ctttggcatc
    29581 tgctttcctt ggttcgttag gtggggggaa atcttttagc aacaatctct tagtctatta
    29641 tgccgtcctt tttggtgggc aagctgtgat tgtcgatcca aaaggagaaa gaggcggttg
    29701 gaaagaaacg ttgccagaaa ttgcggatga aattaatatt cttaacttga ccagtaagcc
    29761 agaaaatcaa ggcttacttg atccctatgt gattatgaag aaaaagaaag actcagaaag
    29821 tttggcgatt gatatcctga cctttttaac agggatttcc agtcgggatg gggagaagtt
    29881 ccctgtgtta cgcaaagcca ttcgaacggt tacccaaagt gatcagcgag gattattaca
    29941 agtaatcgtt gaattgcgta aagaggactc accagtcgcg gaaaatatcg cggatcacat
    30001 cgagtccttt accgattatg attttgccca tttgcttttc tcagatggtt cgattaccca
    30061 gtcgattagt ttagataagc agttaaatat cattcaagta gcggatttgg ttttaccaga
    30121 tgcagaaacc acttttgaag aatataccac catggaattg ctgagtgttt ccatgctaat
    30181 tgttatttcc acttttgcct tggactttat ccatagcaac cgagaaattt tcaagattgt
    30241 ggacctggat gaggcttgga gttttcttca agtagctcaa ggaaaaacgt tatcgaataa
    30301 attagtgcgt gctggtcggg ccatgaatgc cggtgtttac ttcgtcacgc aaaacagtga
    30361 tgacttgttg gatgaacgtt tgaagaataa tattgggcta aagtttgcat ttcgcagtac
    30421 cgatattcat gaaatcaaga aaaccttgga attctttgga ttagataaag aagatgaaag
    30481 taatcaaaaa tgcatgcgag atttggaaaa tgggcaatgc ttgatgcagg atttgtatgg
    30541 tcgagtaggt gtggtccaag ttcatccagt ctttgaagaa cttttccatg cttttgatac
    30601 ccgtccgcca gttcaagaga gaatggagtg aggagatgaa aaagaaactt tttcgtatct
    30661 ttggaattgt tttaatcagt ttggtcactc tgataatact tttgagctta gttggaacgg
    30721 tagctgaggc aacaggtctg gttgatgata cagttggagc cggaaatctt tattcccaat
    30781 attcacttgg taactatcag ttggatttct ttgtggatag ttcgtgggat tggctgcctt
    30841 ggaattgggg tgatggccta gggaagagcg taatgtatgg actttatgcc attactaatt
    30901 ttatttggac agttagtctc tacttatcaa atgccacggg ctacgtggtt caagaagcct
    30961 ataagctgga tttcatttca gatacggctg aaagtattgg gaagaatatc caaaccttag
    31021 ccggcattac cgaaaatgga ctccagacct cgggctttta ctttgggttt ctattattga
    31081 tgattttagc tctaggagtt tatgtggcgt atacaggatt attaaaacga gaaactacca
    31141 aagcagtccg ggctgtcatt aattttgtga tgatttttct actttcgggt tcgttcattg
    31201 cgtatgcgcc cacctatatc acaaaaatca atgattttag ctcagatgtc agcgaagcgg
    31261 ctcttagcct tggaaccgag attgtcgtgc cgaattcaga aagccaagga aaggacagtg
    31321 tggatttaat ccgagatagc ttgttttcca ttcaagtcca acaaccgtgg ttactcttgc
    31381 aatttgatga ttccaatata gaagaagttg gcgaagatcg tgttaacaaa atcctgtctg
    31441 taagtccaga tgaaaataaa ggcaaggatc gggaagaagc ggtcaaagcg gagattgaag
    31501 ataatgacaa tgcgaatctc agtattacca agaccatgaa ccgcttaggg accgtggtct
    31561 ttttggtgtt gttcaatatt ggcatatcct tctttgtctt tctcctaaca gggattatgt
    31621 tgttctcgca aattctcttt attatctttg cgatgttttt gccgattagt ttcttattgt
    31681 ccatgttgcc gacttatgag agtttgggca agaaggcgat tattcgcttg ttcaatacga
    31741 ttatgatgcg agcaggagtt actttggtga ttacgactgc atttagcatc tccacgatgt
    31801 ttttcaatat ttcggcaacc tatccatttt tcatggttgc ttttctgcaa attgtgacct
    31861 ttgccggtat ctatttcaaa ttgggcgaca ttatgaatat gttcaatctc cagagtaatg
    31921 atagccaatc aatgggaaga cgggtcatgc gaaaaccgca aatgttaatg aatcgaacat
    31981 tgcggcaact caatcgaaat gtgggacgga ctttggcttt tggtggtggt gcagcagttg
    32041 gaaataagtt ggcaaaggaa caatcaaaat ccaaatttaa gccatccggt tcttctctac
    32101 gaaagaattc acgattaccg aacgatcacg agataccatc tgacttgaat aaggaaaagc
    32161 caattccaaa taataagaaa caatcccgaa tgaatttggc cgggagaaaa gtcggcaagg
    32221 ttctggatac ccaagctttg gtgaaagata agaccaaaca agtaaaggat ggagtacgga
    32281 atacgccaac caacttgaaa tacaacttcc ataaaggaat tgaaaaaacg aaaaaagcac
    32341 cggaagagtt taagcgagga ctggtccaag aaaaagcaaa tcgaggagag ttgcgagaaa
    32401 aacaacaaca acgtagagac gaaaaaatag ctgaaaagcg caaagtattg aacgaaatag
    32461 ggaatcctca tgggaaaaga agggaaaatg ttccgataaa agcaaaggtc caatctcaaa
    32521 aggatagagc accaaagcgg attgtgagag caaattcgaa tcccgaaatc aaacgaaaat
    32581 tcgctagcca agaaatcatt tcaaaaggaa taaaccaatc ctcaagtaag aaaaattctt
    32641 ttcaacaagt gaaacggcgg tctactctgt ccaaacgaac caatcgaaaa gtacaaaaaa
    32701 gtttgaaccc tcatcccaaa ccaaggtcag gtgagaaaaa atgaacgtaa agaaattaag
    32761 tgccatttcg ttaactttcc taacaatctc ttttatgggt atcttgatgt gtgtgggctt
    32821 attgtttggt gaagacagtg aaggaagtag tagttcaggt gctcctcctg gggggagtag
    32881 tgtctcagaa gaggttttga aacaccgtgc aatggtggaa aaatatgcca aagaatcgga
    32941 tataacagag tatgttccga ttcttttagc gattattcaa gtggaatctg gtggcacaat
    33001 ggaagatgtg atgcaaagtt ctgagtcagc cggtttacca ccgaattcat taagtaccga
    33061 ggcatccatc aaacaaggat gtctctattt tgcgtctttg gttaagcgtt ccaaagaatt
    33121 aggttgtgat caagatagta tcattcaggc ctataacttt ggcggtggtt atttggacta
    33181 tgtggcaaaa aacgggaaga agcatagttt tgctttagct gaatcttttt caaaggaaaa
    33241 atcttgcggt caaaaaatcg attaccctaa tcccatcgcg aataaggaaa atggtggatg
    33301 gcgctataac tatggcaata tgttttattg cttattggtg aagcaatacc taactacaac
    33361 acagtttgac gataaaacag tccaagggat ttttgacgag gcgtttaagt atgaaggaac
    33421 ggcttatgta tttggtgggt caagacccga aacaggattt gattgtagtg gcttgactca
    33481 atggtgttat gccaaagttg gtctgaaact tcctcgggtg gctcaagatc agtatgatgc
    33541 catgacccat attgacctaa aagatgccaa accaggggat ttggttttct ttcattcgac
    33601 ttatgatgcc ggtacttatg tgacccatgt cggtatttat gcaggggaga atcgcatgtt
    33661 tcatgcagga gatccagtag gctggacgaa tctaacagaa agctattggc agcagcatct
    33721 tatcggtgct ggacggtaca aataagagaa aagaggaaaa agaatgaaga taaaaattga
    33781 acggaatcaa aaggaaaaga gcctaaaaaa gaaaaaagaa cgagtcgtat ccgtgggtaa
    33841 gcatcgaaaa atggtgcttg ccctttggct tttattatct tgtagtctta cttttggcgt
    33901 ttataaaaat ttcaccgcca ttgatcaaca tacaactcat gaaaaagtgg ttattaaaga
    33961 gaaagtagtg aatacttctg gtattgaaaa ctttacgaag gatttttcga aagaatattt
    34021 ttcgtggaaa aataacaaag aagcaattga aaagagaatg aacaacctcg aacagtacct
    34081 tacagaagaa ggtttcgctt taagtcaaga tatggttcga gcagatattc ccactaattc
    34141 cgaagttcag tcagtaaaag tcttagatgt tgaaaagagc tcggaggaat ttgtcgtttc
    34201 atttttagtg gagcaaaaga ttacagaggg aaaaaaggca caatcgattt cttctgcata
    34261 ccgagtgacg atctttgaag atgaaaacag gaattatata gtaacgagtt tgccaactat
    34321 gattgcaaaa cctgataggg caaaatacaa gacaaaacaa gtagaaaatg attcgaaaat
    34381 tgatgccaaa actacagaag aaatcgctga gtttttggaa acgttcttta aattatatcc
    34441 atcagcttct gaaaaagaat tggagtatta tgttgaaaat gatgcgatgc gaccaatcaa
    34501 tattgactta aagttttcga agttagagga tccagtttac ctacaaaacg gaaaaaaaat
    34561 tctagtgagt acaaagataa aataccttga taatgtatca aaaaccacta gctactttca
    34621 gtacaaatta atattacaaa aaaatggaaa ctggaaaatc attgatgtta aataataatg
    34681 actcaatcta aatttatgag aagttataag tagtttaact tatttcaggc agaagtcgcc
    34741 catcctctat aggtgggtga tgaatgccgt tcggtaactc gaaaaccgac aattaagaca
    34801 tagtgccttt ttattttaat ccatgtcata atcagtctat ataagaaatg gttgatatac
    34861 catggaatta gatacaaata atcattcatt atttcttctt tgttaccacc ttattttagt
    34921 agtgaaacat tgtaaacatc tcattgatga tgtaatgtta gcttatgcaa aaaactatct
    34981 ttgaaagaat atcagaatca catcacatta ccttagttga gtgaaatcgt gataaagact
    35041 actttcatat tatgctcaaa gcacaaccta aaaccgaatt aacaaaattc agcaatgcct
    35101 aagctcatgt ctgataaaat gagactttct aagggtcaaa aatttccttt gaatacgatc
    35161 attagcttag ataaaagtaa aaaccttaga actacgggga tagcttggtt aataagagat
    35221 atcgctgtta ataaagacat aagcttgcaa gtatgctcta ttcccaagaa tctcccactt
    35281 caagcactaa ggatcgttaa attcagctaa gtggcaagta gttcactaga cgataattat
    35341 ctccatattt caatctcagc ttttccattt ttcatagcaa attccgttat ttttatagca
    35401 tctaaaagat tcagcttttc cactgatctt tcaccacttt tcagcttttt tattgtgctt
    35461 aaattgatac cagtaccttt tgatattgaa ttggaagtaa aatcattaag ggtttttttt
    35521 attaaattca tatccgcaat tccagcgttc ataagaatga agattctcct ctcatagact
    35581 actttgaaaa tggtactaca gtggctgttt atatatttca gtaggttact gtagtggtca
    35641 ttttattata attagaaaag gaacctaaat caaaaatgct aaagaaaatt ttcatagtat
    35701 taaacctatc atgactaaag ccacggaatt cttgataaaa gagtatactt gcaagcctat
    35761 gtctttacta acagcgatat ctcttattaa ccaatctatc cccgttgttc ctaaggttct
    35821 tgatgttttc taaggcaatg cttgtataca tagcccttga tcaaaatatt gatactaacg
    35881 ttgacgtttc gatcatgatg tgtacggcaa ataggacaag tccattctcg aattgtgaat
    35941 gacttcttac catctgtgtg tccacattct gaaccaattt gactagacgg aaatcattta
    36001 tctatcttga tgattttttt gacagaagta tcgaaaatct ttacataatc tgggtgttgc
    36061 attcttatgc agatgagagc gttagctccg tattcataac cagttgcctc taaaacgtag
    36121 tccatcggtg gatacctagc ttgcggttgc cagtggcttt cgcagtcttc ttgtaatcaa
    36181 atcgagcatc taattgagta ctagtatttt gaagataata cttgagtttt tcggttttca
    36241 gaagttcact tcatcttcca tcatccatgg aaatcccact cacccatacg caggcctcca
    36301 aaaattcttc aacaatggtt tttcagacat ttttctgaat acctccttgt tttttgatac
    36361 gccagatagt caggaaaaag gcaacaaaac acaaaacaaa gctgcctaaa aaggtaatct
    36421 tcattccata taaaaagaca tccggccgat ctgcgatata gttagttact cgttggccca
    36481 tcttagcaga catcgcttga tataagaccg tcgtagaaag cgaaatcccg atgaccatcc
    36541 ccaaatttcg ggcaaaggaa ttcatggagc ctgccagtcc caaattttcg atagtgacag
    36601 aactcataac catcgtatta tttggggact ggaacaaggc attgccggca cccatgatgg
    36661 cagtagcgac aatgtacagc cacaaaggag agtccactga caaaaacata tacatgactt
    36721 gggttaccga caataaaccc aagcctacta atacaaaaat ctccgggcca acccggtcgg
    36781 tcaggtatcc gcttaaaggc gccccgacga ccatcatcaa gggaaagacc atcatcaaca
    36841 gtccggcata gctaggactc aacccgcgag cattttgcag ataaaaagga atcactacat
    36901 taacaaagaa gttggaagaa aaaatcaaca aggctgtaat caagctcatg gtaaaaatct
    36961 tgttcttaaa aataccgaaa gaaatcaatg gagaggctgc tttcttttca acttttaaaa
    37021 agctgacaaa ggcaatcact gaaattacga acagtacaag ggggactgga tggaaaaagc
    37081 cactttcttg ccccaaaaag attgttccaa aaaggctgat aatcgtagcc gcaatggcag
    37141 caaagccccc tccatcgatt gtatggccgc tcatggtgat atcttttggt aagaattttt
    37201 cagcaatcaa catcgttacg attcccaccg gtacattgat ccaaaaaatg tacgcccaag
    37261 aaaattggga caaaatcaag ccgccaagac ctggtccagc aatcgagcct aatgaaacaa
    37321 gcgcaccgat tgctcccaat gcccgaccac gttcatgtat gggaaagact tccgtgatga
    37381 ttccggcatt tgtagccata gtcatactag caccaatggc ttgtaccacc cgtgcgaaca
    37441 attaaatacc tagcgaatga ttgaacccac acaacagcga gcccaagaca aagatcagcg
    37501 taccaatccg aaatactttg attttcccac agctatcgcc aattttacca aacagcaaca
    37561 aacaagaaca gacaatcatc agataaacgg acactaccca ttccgcttga ttcattggta
    37621 tgcccatatc atctgagatg gtaggtagtg caatattgac aatactgcca tccaatgtgg
    37681 acatgaaggt aaacatagat atagcaatca aaatccacca gcgatttttt tggacttttg
    37741 catcttcttg ataactatgc ataataggat ttcctttcta atgatcgtgt agctaagaac
    37801 aatataacct atgtctactg cccaacaact tctcgcgact ctagccatag gaagcttgag
    37861 taatttcgat ttgttattct catgtaacca tgtaagtttg agatgcttta tttgaactta
    37921 ctctatagta actagtgata attcgcttta ctttcttcta gcaatttcta aaagtcgact
    37981 tcattgttct tcagattaat agtgtgagct atctcgtgag acgtaagaaa atttccattc
    38041 tactttttcc caattttttc atatttatgg tgttgcacct atttgttttc caatttgctt
    38101 ttcaaataca ttgctatgat attcaattcc ataggattat actaaatgtc aatagtatga
    38161 ataatatatt atagatgttt atatatcact gttaattatt atccacaata attaatgatt
    38221 acctcgatat ttgtcaatca aaggttgaaa caatttattt tcagcatctt ttctaacttg
    38281 agctgcactc ttaatagaat catacgatcc taaatagtac ctcttttttt taaagttata
    38341 tacgcaatcc atttctcttt agaacgataa actcccttaa caccacttgt attatttttc
    38401 ggtaattttg acgttagaca ctctattact gtcccttcaa cacatgtcct tgtttcttgc
    38461 ctttttaaac atccacaact ttgagtatgt ccaaaaaata agttactata acttatctca
    38521 cacatactgc cacactcaca ttgacaaacc cattttgttt tccatgaaca gttattgtcc
    38581 aaaggatact ttgcagtaag taatccgaat tttttatttt caatgtgatg cttttttgat
    38641 ttcaaacatc cacagctttt tacatcacct tttagaagtc gctttgccga tactaacttt
    38701 tttctcccac acagacattg acattcccaa acaacagttc cttcacttcg cagtttagtt
    38761 ggcttgattg caactaactt accaaaagtt ttatttgtca aatttttata tttatgccta
    38821 tttttataat ttccacatcc acaacttttg gttctacctg atagtaagtt tgatcgatat
    38881 acaatcgttt cattcccaca gtcacatagg taacgatatt ttttatatcc attatgagta
    38941 atctcctcaa caacagtaag gtgaccatat ctttccttca ctgcacctac cctcccaaga
    39001 aagaagtacc atataatttc aattaacttt taataaatca gaaaaaaata ttttcaattt
    39061 ttcttagtct gattttatga ctaatttata atacaagact gaacgatacg tacacgaaat
    39121 cctcaagata gataaattgt tttcatctag taaaacctgc tcaaaatatg agcataaaga
    39181 cggcaataat acactcgata ttcgataatg gacttgtttt atttgtcaaa ctcatcgtaa
    39241 tcgagatatc aattccagtc tcaatatcct gatcaaaggt ctaagaatcc aaccattttc
    39301 ttgttacttt gtcagattga ttaccttttt tagtctccga ataataaggt tttcggatca
    39361 actaacatgc tctgagaaag actactatct ttgcaaattt ccaaatgaag gtgtgggcca
    39421 gtactattcc cagtacttcc tacatagcca attatttgtc cttgcaaaac attatctcca
    39481 gtttttacta ggtattgttg ctggtgagca tataacgcgg ttgatccatc ttcgtgttgt
    39541 atcaccacgt aatttcccca agagtagtga aactctgctt taacaacctt accagcttta
    39601 actgcatgaa tcggctcacc ctgtgtagct gccatatcaa gtcctcggtg aaattcattt
    39661 ccacgatttc caaacaaact ggttattgaa taattatgaa taggcacttg gtatccagtc
    39721 ttactcattt gtggaccttt aacttcttta tcatagttgg ttaaatcata ggttttaatt
    39781 aattcattta gcttcaaata gtattgtgta tctgttgcat accgtcctgt aaggaattta
    39841 gttgcttctc gatatgtttt actattagct tttaatgctc ctgcataaaa atctttattt
    39901 ccactaattc ccttagttaa taatttggca taatcttcaa aactttcctc atagttttca
    39961 tacactctaa attttgattg ggtcgtataa agcgttccat tacccaaatc ttcgtttgtt
    40021 accataatga caaagtttcc attataagtt ccttttatcc caaataaatt gtaattcggt
    40081 gcctgagcta actggctttg accactggca gattctaaga tcgcttgtgc aatcataaca
    40141 gaagcatata aatcatattt ctgaccaatt tttctcgcag actcaccaat tttcataata
    40201 aaattttcta cagattcatt tttgtcaaaa tgaatttctt cattaccaga tagtattggt
    40261 tgtgattgga aagattctgt tgattctatt tgctgtggtg tcgaaaaatt ttttgtttgt
    40321 ccttccgttt ttggcgcttc cttttttgaa gactctatat ggtttggtct tgtcactttc
    40381 gaagaagtta tttcacttgt actacttgaa gttgttgaag attcggcact tgaaatcgta
    40441 ctactttcac ttgacgaagt tgttgaagac tcggcatttg aagtcgtact actttcactt
    40501 gacgaagttg ttgaagactc ggcatttgaa gtcgtactac tttcacttga tgaagttgtt
    40561 gaagactcag catttgaagt cgtactactt tcacttgata aagttgtcaa aagttcacta
    40621 ctctctgctg ttacatcgga attactcatt tcagtcgtct cggttctttc ttttgcaatt
    40681 ttgtatgttg aattttcgct tgccaacaca tggaaaggtg ttgctataaa gagttgggac
    40741 attaatataa ctgctgatgc aagttttaaa gattttttca tttgatttac ctctcttttt
    40801 tctctttaat agtgttttct attaaaaatt atgataaata ttttcaatga attgagtaat
    40861 acagtatatt tcttgatttc atttagtaac gattatgttt caatgtttaa tcctgttact
    40921 gcctttaaaa taaaattata gggttggcta aaactaggat gaaataaata atctaacatt
    40981 ttcaatttgt ctaatgtaat ttgttcttct atagcaagag aaaacattga taatagtcca
    41041 gataaatctt cttgactaca aagttgtaca cctactattc tctttgtatt tttttcaaag
    41101 ctaattctta attttacacg actatttttt ctcaaaaaaa caggtctaat gaaatcttct
    41161 atttctatat aatctataga ctcacttaat aatcgtgctg tttttaaatt caaacctgct
    41221 gcgtaaaaat tgtaattaaa tatagaaaag ccatttgtaa attgggaacc agaaggtgta
    41281 aaatcttgat tgtcaatatg ttgagttgca atataagctg ttcttagagc attagaaata
    41341 gaaaaatcaa cactacttct attaatggac gtaaaaaaag ttgtagcaca atccccaata
    41401 gcatatatat cttttatatt tgtttgctgg tattcattag ttagatatgc tccactaaca
    41461 tctctcaaga gttccttttt gccaattgga ctattagata gaaatcctat agcttgtacg
    41521 accatatcca tagagtattg ggtaccactt gtagtgacta ttttttcaat cttttttctt
    41581 cctaaatatt ttttaggact ttctgataga caaagatgta ttccattttt tgatagtatt
    41641 tcttcaactt tatctgaaaa actacggtcg taataagtcg acaacactct atctgcaaca
    41701 tcgaaaagat atacttcttt ccctcttttt cttatggctt cagcaatttc aactccaata
    41761 tatcctgctc caataattcc tactttttta atagtattat catctaaagc tttaaaaatc
    41821 tctttagcat ttggaaaatt ctttatcgaa aaaatattag ctaaattatt ccccggtaca
    41881 tctaacataa tttgctttga acctgtagct agtataagtt tgtcatattc ttgggaaaat
    41941 cttgtgccat ctttttttac acaaataatc tttttatttt gaatatcaat tttgtcaact
    42001 gctgtctcca aaaacaactt ttttatctta gaatttagtt ctcttctatt ttgataaaaa
    42061 agatcttcta aattgctaac ttctcttttt atgaacaacg atgtcccaca ttctaagtaa
    42121 ctaatttctg attttttatc tatcagaata acttcataat ttgaatcaca taagtttaga
    42181 aatcgagctg cagctagccc cgaatggttt gatccaataa ttactatttt tttcacttaa
    42241 tcacattctt ttcccttcta tgaaaagtat cttcattttt aactaacaac ctatctccca
    42301 aaataaaaaa caaaaagagg aagaaaaaga ttcttcctct tttcagagaa gatttaagaa
    42361 attgattttc tattcattct ctttttttcg atttttatac cctaataacc ctaaagttcc
    42421 taacaggatt aaaccaccac taatagtaaa gatattacta tcaatctctc ccgtcttagg
    42481 ataagctttt gaggactgct tttgaggact gcttttgcct ctatttttgt tttgcttgct
    42541 accttgattg tcagtcttat tatctttact aacggctttt tcactgtctt tttcgtttga
    42601 agaaacaact acattagtag gttcactagt attaccagat tcatcagttg cagttactat
    42661 cacttttgtt cctgcctcta atttaggaac tttaactgta aaattaccat ttttatcagc
    42721 tgtcccatgg tattgactcc cgtttggtaa tgtaactgtc acctctgcat ttggttcagt
    42781 tttaccagta acaatttggt caccctctgt aataggatca acttgaggtt tattcggtgc
    42841 ttttttatct tctacagaaa tagtaacctc tacttcttct ttagatccat ctggatatgt
    42901 tactactaca atcccttttt tgtttccagc tgtactaaca tctactttct ctttccaagt
    42961 ataagtagtt ccttttggta gatcttcttt gttcttaatt ccctcagatg cgtctggctc
    43021 tttatttaag tctgtagtca ctttttgtcc ttcaggatt
//
LOCUS       DACIRY010000007        42130 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.007, whole genome shotgun sequence.
ACCESSION   DACIRY010000007 DACIRY010000000
VERSION     DACIRY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.007"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(52..2850)
                     /gene="ileS"
                     /locus_tag="IYE22_000299"
     CDS             complement(52..2850)
                     /gene="ileS"
                     /locus_tag="IYE22_000299"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ9505541.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(3112..3819)
                     /locus_tag="IYE22_000300"
     CDS             complement(3112..3819)
                     /locus_tag="IYE22_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ9505542.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(3840..4622)
                     /locus_tag="IYE22_000301"
     CDS             complement(3840..4622)
                     /locus_tag="IYE22_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ9505543.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(4671..4958)
                     /locus_tag="IYE22_000302"
     CDS             complement(4671..4958)
                     /locus_tag="IYE22_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ9505544.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(4973..5572)
                     /locus_tag="IYE22_000303"
     CDS             complement(4973..5572)
                     /locus_tag="IYE22_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ9505545.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(5586..6263)
                     /locus_tag="IYE22_000304"
     CDS             complement(5586..6263)
                     /locus_tag="IYE22_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ9505546.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(6280..7521)
                     /gene="ftsZ"
                     /locus_tag="IYE22_000305"
     CDS             complement(6280..7521)
                     /gene="ftsZ"
                     /locus_tag="IYE22_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ9505547.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(7544..8869)
                     /gene="ftsA"
                     /locus_tag="IYE22_000306"
     CDS             complement(7544..8869)
                     /gene="ftsA"
                     /locus_tag="IYE22_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ9505548.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(9025..10245)
                     /locus_tag="IYE22_000307"
     CDS             complement(9025..10245)
                     /locus_tag="IYE22_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ9505549.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(10260..11348)
                     /gene="murG"
                     /locus_tag="IYE22_000308"
     CDS             complement(10260..11348)
                     /gene="murG"
                     /locus_tag="IYE22_000308"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ9505550.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(11374..12735)
                     /locus_tag="IYE22_000309"
     CDS             complement(11374..12735)
                     /locus_tag="IYE22_000309"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ9505551.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(12749..13711)
                     /locus_tag="IYE22_000310"
     CDS             complement(12749..13711)
                     /locus_tag="IYE22_000310"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ9505552.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(13737..15929)
                     /locus_tag="IYE22_000311"
     CDS             complement(13737..15929)
                     /locus_tag="IYE22_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ9505553.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(15930..16331)
                     /gene="ftsL"
                     /locus_tag="IYE22_000312"
     CDS             complement(15930..16331)
                     /gene="ftsL"
                     /locus_tag="IYE22_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ9505554.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(16336..17295)
                     /gene="rsmH"
                     /locus_tag="IYE22_000313"
     CDS             complement(16336..17295)
                     /gene="rsmH"
                     /locus_tag="IYE22_000313"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ9505555.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(17316..17747)
                     /gene="mraZ"
                     /locus_tag="IYE22_000314"
     CDS             complement(17316..17747)
                     /gene="mraZ"
                     /locus_tag="IYE22_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ9505556.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(17917..18285)
                     /locus_tag="IYE22_000315"
     CDS             complement(17917..18285)
                     /locus_tag="IYE22_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ9505557.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18456..19412
                     /locus_tag="IYE22_000316"
     CDS             18456..19412
                     /locus_tag="IYE22_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ9505558.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            19485..19601
                     /locus_tag="IYE22_000317"
     CDS             19485..19601
                     /locus_tag="IYE22_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ9505559.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(19656..21338)
                     /gene="recN"
                     /locus_tag="IYE22_000318"
     CDS             complement(19656..21338)
                     /gene="recN"
                     /locus_tag="IYE22_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ9505560.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(21356..21805)
                     /locus_tag="IYE22_000319"
     CDS             complement(21356..21805)
                     /locus_tag="IYE22_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ9505561.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(21912..22730)
                     /locus_tag="IYE22_000320"
     CDS             complement(21912..22730)
                     /locus_tag="IYE22_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ9505562.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(22739..23629)
                     /locus_tag="IYE22_000321"
     CDS             complement(22739..23629)
                     /locus_tag="IYE22_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ9505563.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLTRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(23631..23855)
                     /locus_tag="IYE22_000322"
     CDS             complement(23631..23855)
                     /locus_tag="IYE22_000322"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ9505564.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(23860..25197)
                     /locus_tag="IYE22_000323"
     CDS             complement(23860..25197)
                     /locus_tag="IYE22_000323"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ9505565.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(25198..26052)
                     /locus_tag="IYE22_000324"
     CDS             complement(25198..26052)
                     /locus_tag="IYE22_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ9505566.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(26149..26598)
                     /gene="nusB"
                     /locus_tag="IYE22_000325"
     CDS             complement(26149..26598)
                     /gene="nusB"
                     /locus_tag="IYE22_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ9505567.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(26591..27016)
                     /locus_tag="IYE22_000326"
     CDS             complement(26591..27016)
                     /locus_tag="IYE22_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ9505568.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(27262..28326)
                     /locus_tag="IYE22_000327"
     CDS             complement(27262..28326)
                     /locus_tag="IYE22_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ9505569.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(28498..28743)
                     /locus_tag="IYE22_000328"
     CDS             complement(28498..28743)
                     /locus_tag="IYE22_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ9505570.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(28882..29256)
                     /locus_tag="IYE22_000329"
     CDS             complement(28882..29256)
                     /locus_tag="IYE22_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ9505571.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(29339..30253)
                     /locus_tag="IYE22_000330"
     CDS             complement(29339..30253)
                     /locus_tag="IYE22_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ9505572.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(30274..31077)
                     /locus_tag="IYE22_000331"
     CDS             complement(30274..31077)
                     /locus_tag="IYE22_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ9505573.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(31110..32099)
                     /gene="manX"
                     /locus_tag="IYE22_000332"
     CDS             complement(31110..32099)
                     /gene="manX"
                     /locus_tag="IYE22_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ9505574.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(32202..32693)
                     /locus_tag="IYE22_000333"
     CDS             complement(32202..32693)
                     /locus_tag="IYE22_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ9505575.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(32884..35718)
                     /locus_tag="IYE22_000334"
     CDS             complement(32884..35718)
                     /locus_tag="IYE22_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9505576.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            complement(36125..36418)
                     /gene="rpmA"
                     /locus_tag="IYE22_000335"
     CDS             complement(36125..36418)
                     /gene="rpmA"
                     /locus_tag="IYE22_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ9505577.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(36431..36775)
                     /locus_tag="IYE22_000336"
     CDS             complement(36431..36775)
                     /locus_tag="IYE22_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ9505578.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(36802..37110)
                     /gene="rplU"
                     /locus_tag="IYE22_000337"
     CDS             complement(36802..37110)
                     /gene="rplU"
                     /locus_tag="IYE22_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ9505579.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            37392..38033
                     /locus_tag="IYE22_000338"
     CDS             37392..38033
                     /locus_tag="IYE22_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ9505580.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(38095..39726)
                     /locus_tag="IYE22_000339"
     CDS             complement(38095..39726)
                     /locus_tag="IYE22_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ9505581.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(39849..40208)
                     /locus_tag="IYE22_000340"
     CDS             complement(39849..40208)
                     /locus_tag="IYE22_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ9505582.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(40367..40440)
                     /locus_tag="IYE22_000341"
     tRNA            complement(40367..40440)
                     /locus_tag="IYE22_000341"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40404..40406),aa:Arg,seq:tct)
     gene            complement(40749..41432)
                     /locus_tag="IYE22_000342"
     CDS             complement(40749..41432)
                     /locus_tag="IYE22_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ9505583.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(41560..42078)
                     /locus_tag="IYE22_000343"
     CDS             complement(41560..42078)
                     /locus_tag="IYE22_000343"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ9505584.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT        11818 a         9212 c         7041 g        14059 t
ORIGIN      
        1 tgataacaaa agggggtgtg acaaagaaca tgtcacaccc ccttttccct tttacttttc
       61 ttcgaatcct tcagctacag cttcaggata gaagtcttca acgatttttg cacaacgacc
      121 gcatagatga ggaagttttt catctactcc tacatcttta cgaacttggc gacagcgatc
      181 gcaagtttcc ccgtctgctt tttccactgt aatagcaaca tcatcaaata ccatagcgtt
      241 atctggcact tgttcatctg ctgccataac ttcaaagtag tctggtgaaa cgatcaataa
      301 ttgtgcaata tctgcatcca aagcttttaa catttcacga acttgttgtt ttggataaac
      361 agttagtttt gcttccaaag atttaccaat caatttgctg tttcttgctt cttccaatgc
      421 ttttaagaca ttatcacgga agtccatgaa agcagtccag gtatccatca attcttcttg
      481 gtttgcaaac tcttcataac caggaaactc gcttaactga acgtactcct cttcttcttt
      541 caggaatgac cagatttctt ctgctgtatg cggaatgata ggagtcaata attttgtcaa
      601 agctacagct gtttgataga aaacagtttg catgcaacga cgttgataat catcttctgc
      661 ttcaatataa actacatctt tagcaaaatc caggtagaat gacgatagat caaccgtcaa
      721 gaagttcata actgtacggt aaatatgcat gaagttatat ttgtcgtacc cttcttctcg
      781 gatttctttg attacttggt tcaatcgaac ggtcatatat ttgtctacag aacgtaaatc
      841 attgtacgaa acagtatctt ttttaggatc gaaatcttct gtattcgcta ataagaaacg
      901 catcgtattt ctgattttac gatatacttc tgccacttga cttaagatat ccatagaaac
      961 acgaacatct gcttcataat ctacactaga tacccataag cgaaggatat ctgcaccaaa
     1021 ttgtttgatc actttttctg gtgcaatgat gtttcctaaa gatttactca tcttacggcc
     1081 ttcaccatct aaagcaaatc cttgagaaat aactgatttg tatggtgcta caccattgat
     1141 agccacacta gtcgtgatac ttgagttgaa ccagccacga tattggtctg atccttctaa
     1201 atacatatct gctgggaatg ataattctgg tcgctgacgt aaaacagctt catgagaaga
     1261 accagaatca aaccagacat ccatgatatc tttttctttt gtaaattcgc cattaggaga
     1321 acctgggtgg gtaaatcctt caggaagaag ttctttcgct tctttttcaa accagataat
     1381 tgatccatgt tcagcaaata attttgccac atgttcgatt gtttctggtg tgatgattgc
     1441 ttctccattt tcagcataga aaatcggaag tggtactccc catgcacgct gacgggaaat
     1501 tacccaatcg ccgcggtcac ggatcatatt atacaaacgt gttttacccc atgggataac
     1561 ccactctact ttttctactt catccaaaat attctgacgg aatttatcga tagatgcaaa
     1621 ccattgtgga gttgcccgat agatcacagg ctttttcgtc cgccaatcgt gaggataact
     1681 atgagtaaag aaatctagct tcagcaaagc tcctttttct tttaatagat cagtgatcat
     1741 aggatttgct ttgtcataaa agataccttc aaatccaggt gcttcttctg taaacaatcc
     1801 ttttgagtct acaggtgaca ctacagatag tttatatttg ctaccagcga tatagtcatc
     1861 ttccccatgt cctggagcag tatggactaa tccagtacca gcgtctaaag taacatgatc
     1921 gcctaaaatc acaagtgatt cacgttcata gaatggatga cttgcagtca tatattccat
     1981 ttcttgtcca gcaaattctt ttagaactgt tacattttcc caaccgattt ctgattggac
     2041 tgtttctagc aaatctttcg ctacgacaaa cttacggcca tcggcttcca ccaatacata
     2101 cgtaaatttt ggatttacag caataccttg gtttgctggc aatgtccatg gtgttgtcgt
     2161 ccaaatgaca aaagctgtat cttcatcaag aatatctttg ccgtctttga cattgaaagc
     2221 tacaaaaata gatggagatt ttacatcttt atattcaatt tcagcttccg ctaaagaaga
     2281 ctcacttgac ggagaccagt aaattggttt caatcctttg taaatatagc ttttttcagc
     2341 cattttacca aatacgcgta cttgtgcagc ttcataagaa ggatctaatg taacataagg
     2401 atgttcccaa tctcctgcaa ctcctaggcg tttgaaatct gctcgttgct tatctacttg
     2461 ggaaagtgca tattcacgac atttttccaa gtattctgcc atggacattt ctttacgttt
     2521 cacgccttta ttagccaata cttgttcaat cggcaatcca tgtgtatccc atcctggaac
     2581 atatggtgca cgatagccag acattgattt tgaacgaatg ataatgtctt tactgatttt
     2641 attcaatgaa tgtcctaaat ggatattacc attggcatat ggaggtccat catgaagaac
     2701 aaagctaggt tttccttcgt tcaactcttg tcttttttgg tagattcgat tttcttccca
     2761 ttctttttgc cactcgcctt ctcggtttgg caaattacca cgcataggga attttgtttt
     2821 tcctaattgc aaggtttctt tcattttcat tcaaccatac actcctttat ttgttttccg
     2881 gacacaaaaa agcatgttca gccgcaaggg acgaacatgc cgtggtacca cccaaattta
     2941 gttgcatcta tctagtgcaa actctctgtg accaataacg aggtcagccg ctgcttctta
     3001 ctctaatttc ggaaaagcag gtctcaagta gaggatcttt caccatgcag tgtcttaccg
     3061 aacttccact ctcatcggct cgctttaaga ataacaaagt gcttttctgt tctactcgtt
     3121 ttcattttca ccaggcacga tcgagatatc aatagcctgt gtatcaaatg ccgtcatagg
     3181 tgcttctatg actcctgtgt ctgagtttac atcagtatca ttttcattgt caagctgttt
     3241 tgctaaaact tctttgatca cttcatggct gtcagtcacg tagctggaga atggttgtaa
     3301 aatttcatcc cattccggac ttttcacttg ttctaattga gactcaagca tcaagctgat
     3361 acgttgatgg aatactcgtg tttttttctt aagatcttca gtttctgtcg caagctgtct
     3421 tgctttttct gttgcatctg tcaagatttt tcttgctttt tcttctgcat cagttgttaa
     3481 ttgatgtgca cgtttttcag cgttcgcaac taactcatct gctttgtttt gtgcagaagt
     3541 aacgatcact tcggattcct tgcttgcact tgtttttact ttatcagccg tatcttgagc
     3601 tacgatgatt gattggttca aagcatcttt taattcattg aagtactcaa gtttttcttc
     3661 agaatgtttg actgcttttt ctaattctcg atttctttga gtcaattcct catagtcacg
     3721 aacaactaaa tctaagaaat catctacttc gtcttgatta tatccccgca tttttgtcgg
     3781 gaatgtctta ttttggatat ctaatggagt taaagccatg agtaaaccac ctttttattt
     3841 tatttacgta atacaccgaa cagtaccctg tacttttctt ttttcgtctt cccctcaagt
     3901 tcttggatct ggactctgcc ataacctctg actgaaataa tatctaatag ttcaagtaca
     3961 aaatcaggtc ttgtattttc tgtccagttc acttttactt taccagcttc gatcagctgc
     4021 ttggaccgct gtctggaaat attataaatc tcagagatca cactgtctag tcgcatggaa
     4081 ctaactgttc ctctttcttg tgtccagcta tctttcggca taatcatttc tgtgtatttg
     4141 attggttcca agcgtacagc aacctttcca atctttgtta cttgtgcagc aacaaaatga
     4201 tccacctcat ttgcaataaa tacttgccag cgatcaccgt ccgagatgat atcgccaaag
     4261 taagagcgtt tgatccccgt tcccactaaa gttccaagga ttttcccatg acttaggctg
     4321 gcaaatttgc taggataatg gatttcatac aatgctgttt caaaatcttc ctctacaggt
     4381 tcgtaataat cggggaaaat caaacatctt tttctttctg cctgttcata tccgccataa
     4441 aacatgaaac gcaaatcact gctttgtcga atcagtgttt ctaaaatata ggattgtctt
     4501 gggtcaagaa attcggtcag atagggtgca tattgtcctt gaacctgttc aatccaatcg
     4561 cctaccgaat cgataaatgt acgttcatct gcccgaaagt gctggtatac atttgcatcc
     4621 aaaattttcc cctcctatct attctgagaa taaagatttt gttgcttcaa tcaataaact
     4681 aaatggacag ttatccatat aacgatctgt gctaaacctt gtacggctaa gttcaagatc
     4741 aggattgccc ctaaaattgt gaaatccaag ggtccaatcc tcaaaggcag acgatcgaac
     4801 aaactgatat acggttcgca gatcttagct aataatcgtc ctagccctga ttgatacccg
     4861 cctggaaacc aagagagcaa tgcatatacc atcaagataa tgctatagaa ataggctgcc
     4921 cgttgaagta aatgaagtaa tagtgcaata gtataaatcg tagttcctcc tcttacagat
     4981 caaacaaatt cgcatctgca aaagattgag cggttgaact gtcaatctcc atcccagtcg
     5041 gtgtacaaag aaaaatctca tctccaacac gtttgatatc tccatctagt gcataaactg
     5101 ttcctgttag aaaatcaacg attcttctcg cttgtttttc ttctactaaa tgaaaattca
     5161 ctaaggttgc ttctccagca atgattcgtt tagcaatcgc catagcttca gaataagctc
     5221 ttggttccat tacagtgatt tttccagcaa gatttgcttt aggttgtgta gaggttcttg
     5281 aagctgttct ttctttcgct acagtttttg taggtgattg atgtagttca attacttttt
     5341 tcttttcagc cggctgaata ctttcatgcg ttacaggacg tgaagtcgcc gttttttgtt
     5401 tttcatgagt attcgcagca gatacagaat gagttgattg attctgtgtt gtattttttt
     5461 tagttggctg tacagccttt tttgttgttt caggataatc ttggtattcg tactcttcct
     5521 catcagctaa tccgaaaaaa ttagcaattg ctcctctgtt taaaagcggc atttttgttc
     5581 ctcctttatt cgtctttgaa tagcgctgtt cctacacgga taaatgttgc accttcttca
     5641 atcgcaattt gaaagtcttg actcatcccc atacttaatt cactacatgg tgcatgggca
     5701 taaccttttg cttgaacaag atcacgcaat tctttcagcg taccaaacac tttatggatt
     5761 tgctcatcag aagcttcttt aggcgccata gtcatcaacc caacaacctt gatttttgtg
     5821 caggcagcta aagattccac aaacggtaaa agctcatctg gtgaaattcc atgttttgat
     5881 tcctcaccag aaacatttac ttcaataaaa caggataatt ctttctctaa ccgcttctgg
     5941 atttcttctg ctaatcttaa actgtccaac gcatgaaagt aatcaatttc atttacaatc
     6001 aattttacct ttctgcgctg taaattacca attaaatgcc actttatttc aggaaaatca
     6061 aataaatctt gtttcttttc taataattta tctactcggt tttctgcaca attcataacg
     6121 ccagctctaa ccaattcttc agcttgagcg gattcaacag attttgttac agctactaat
     6181 gtaacttctt tcggatcacg atgaacaaga gcacacgaat cctgaatttc ttgcttgatt
     6241 ttctgcaagt tgtcagcaat catgtgctct tcctctcctt tattttttac gacggaaaaa
     6301 cggtggcgta tttaattcat catcgccatg agattttgtt tcttcgcggt tgaatgtatc
     6361 aaattctttt ttttcgatat tgtcaaaatt ggaatcatca actctaggac gaacattttg
     6421 ttcacgtctg atatcccaat cgccaaaact gttttcttct tctgaagaag ttggtttagc
     6481 ttgatccata tctaatacag tcttttgagc aggtgattgc atttgtgctt gtctagctgg
     6541 tcggctagac ttgcgttcct tttttgattc atcgatacct gttgcgatga ccgtcacacg
     6601 gatttcatcg cccatttctt cattgataga tgtacctaga atgatattta catcgccagt
     6661 tgctgcattt gctacgatat ctgaagcatc ttgtgcttcg aacaatgtca tatccaaacc
     6721 gcctgtgatg ttaagaagta cttgttcagc tccatcaata gatgtttcaa gcaatggaga
     6781 ggagattgct tttttcgttg cttctactac gcgatcttct ccactagcca caccgatccc
     6841 catcaatgct gtaccttggt tttccatcac tgttttcacg tcagcaaagt caaggttaac
     6901 gtaacctggc gctgtgatca aatcagagat accttgtaca ccttgtcgta aaacattatc
     6961 tgcttcacgg aaagcttcaa gcatcggtgt ctttttatct acaacttcta ataaacggtt
     7021 atttgagata attaataatg tatccacatt ttctttcaaa cgagcgatac cttctgcagc
     7081 aaaacggcca cgtttcggtc cttcaaaagt gaatggacgt gtcacgacac caacagtcaa
     7141 tgcgcccaat tctttcgcga tacctgcaac gattggtgca gcacctgtac cggttcctcc
     7201 acccattcct gcagtgatga aaatcatatc tgcaccgtct aaagcttcgc gtaaggattg
     7261 ttcactttct tctgccgctt tttgtccaac ttctggctga gaaccagcac ctaaaccacg
     7321 agtatatttg gggcctaatt ggataactgt ttcagctttt gaatttttta atgcttgcac
     7381 gtctgtgttg gctgtaatga attcaacacc tttaacgttt tcttcaatca ttcggtttac
     7441 agcatttcca ccgccaccac cgactccgat gactttgatt actgcgccgt cattgatatt
     7501 attatcaatt gagaattcca tatcttattc ctcccaggat gaattaatca aaaatatttg
     7561 taaagaaacc cttgatttta ttttttacat tttctccgga ctcttttggt tccggctcat
     7621 cgtagattgt ttcttcaggt gcttcatagc tctcatatgt gtttgttgtt tcttgttcaa
     7681 ctaccatttg acgagctgct gaggtttcac ccgtaacagc aatttttgcc aattgataca
     7741 cttcactcaa gtttgctgaa taatccacga tacttattac atttgtaaat accgggtttc
     7801 tcagccccat gtgatttgga acatacaatt ttacatttac gccgaagatt tcttgtgcca
     7861 agtccacaac accaggaaga ctagcagctc cgcctgtcaa tacgacgcca cctggcaact
     7921 ctaatgcttc gatttggtcc aatgcatctt ttgctttgtt gaaaatctgt tccattcgag
     7981 ctgaaataat ttctgacaag tagcgttcat cgactttcac tggttctgac tgaccaatca
     8041 catctactgg aaattcttca tctggtgatg tccgttctgg gtatgcatcc ccgtagttta
     8101 ttttcaacgc ttctgcattg ttaaatgacg tatttaatac aatagagata tctttagtga
     8161 caaattcgcc accttcttgg tcaagattgg taaacttcaa ttgcttatca tgcataacag
     8221 ccgttgttgt ttggccgcca cccatatcaa tgacgatcgt accaaaatct ttttctccgt
     8281 cagaaagaat tgattcagtc agcgctaaag gagtaatcac catttcgttg acgattaatc
     8341 cggcattttc cacgcattta cgtatgttat ggatgatcgt tttaggtcct gtaaacaaca
     8401 gaccgtacat ttctaatctt acgccgatca ttccacgagg atctttgatt ccctcgaacc
     8461 catcgacagt aaaatcttgt ggcaatattg acacgatttg acgttcagga ggtattgaac
     8521 gaacaagggc agctgaagcc acatttctga catcttcatc ggtgatttcc tttgaatctc
     8581 cgttaactgc aatcattccc tggcaatttt ctacttcaag catgtttgct ggcaaaccga
     8641 cacttacgcc tttgatctgg atacctgctt tttcttctgc ttgtcgtact gctcgttgta
     8701 tcgcttggac cgttttatcg atatcaacga taatgcctcg gttaatccct tctgattttg
     8761 cgtttcctac tccaataata ttcatttggc tgtcgatata ttcagccacg acaactttaa
     8821 cagatgtcgt tccaatatca aggcctacat acattcctgt ttttgccatg aatgggattc
     8881 cctcctactt actattccat atacaagcga acaactagcg tccgttttaa aatatactta
     8941 ttaatagata tattcactgc ttccaattct accataaatt actggttttt agaaagtaca
     9001 ttcttacttt ttaaagttgt tttatcaatt ttcatttaaa cttgacggag ttgtacttgg
     9061 attatcttct atttgacttg tttcacttga atcatttgaa tcagatgatc cgtttgtgct
     9121 agacgatcca ggtgtttgtg ctttttgcgt ttctgcctct tcacttgatt cgtttgtttg
     9181 atccgcattt tcttcacttt cagttgtttc ttccgattgg cccgattctt ccgtttcttg
     9241 agtgctaccg taaggatgcg agaagatacc aacttccatg tcgatcacac ctttttcatc
     9301 catatctttg gctacttgcg aataatattt catttggttc gcaagattat tgatatttac
     9361 aataacttga ttgccgtcat tcatattcaa ttggatcaga tttttattgc ttgctcttgg
     9421 cgtgtacttg atttcagaaa ttgctttttg taattctgat gataactgat tgtattgctt
     9481 tgttaattct ttgatttttg cagagtcttt gaactcttcc aagataggga gattttttgt
     9541 aggatttgct actttttcat ctaatactgt tccattttca atcactgggt agtattgatt
     9601 gttttttgcg atcaaagcaa tctctttata ttctgttaca tccaaatcga attcattcat
     9661 ccctttgaaa tgtatatttg ctgtttcgat tcttggctgt gcttttttaa ggcgatcgat
     9721 ataagtagat cgatgaaaat actgtcccca aacattttct cctaatgaca attttgtatc
     9781 agagatgatt tcttggctgt tgactgtctt attgcctgat actgcaactg cttgtaattt
     9841 gctataagga gaaacataat agatcaaaaa aatcagcggt atacccaata cagtgatgat
     9901 caaagctaac cgacgaaata gtactttgtg ccgttgactt tttaaattgg gcagccggtt
     9961 caaaaaagaa ccgttatatg gtccaccgtt tttcttttgc ttgatctctt caaaaaattc
    10021 gatttttttc tcggactggc tttcttcttg ctgttcctct tgaaccgttt tttcttcttc
    10081 agaatctgaa aggggtgtat ctaatgttat gtcttctttt tttggctctt ggtttttcat
    10141 ctcttgttct gccagatatt ttcggttagc cttttgccaa ggagtcaaat tgttttcatc
    10201 agaagacgga tgagacaatt tttttggttc ttttttctta ctcactaaga ccaccgcctt
    10261 tattttttga ttatttgttt tactaagtca taaagacgtt ttgaagcatc agggattcct
    10321 tgttctttag aagctcggca catttgcttt tgcaattctt catcacccat gatttcgttg
    10381 actgtctgac ttagtgattc acctgttagt tcattatctg caatcatttt tgctgctcct
    10441 gcgtgaacaa gactcattgc atttttagtc tgatgatcat tcgtaacata tgggctggga
    10501 accaacactg ccggtaaccc cagagctgtg aattctgcga tcgaagttgc accagcacgc
    10561 cccaccagca gatcactgga agccatgacc tctgccatat ttttgatata aggctggaca
    10621 ctaatatttt gtatttcagg atttatttta tttaggatct cttcataata tctatctcct
    10681 gaagcataca aaacttggta agaagaagga atatattcac ttaaaaaaac tgatactgct
    10741 tgattgattt taagtgctcc ttgactaccg ccaaaaatca agactgtttt cttttctgga
    10801 tccaaatcaa atgttttcaa aatatctgat tttttagtat ctgctacttc ttgtgctcga
    10861 ggattaccta caagaactgc tttgttttca ggaaaaaagg aagccgcatc tttaaatgaa
    10921 agagcgatcc gatccacata tcggcttaaa aatttattgg taacacctgg tacactgttt
    10981 tgttcatgga taactgttgg aatacccatt ttggctgcag cgtacactac cgctccggaa
    11041 acatatcctc cagtaccgat aacgatatct ggttgaaaat cactgaggat tttttttgct
    11101 tgacggatac tcttaagaaa caatcggacc gtcttgatgt tatgcatgga aatttttcgt
    11161 ttaaatcctt gaatctccaa ggtatggaaa ggcattcctg tatcagggac gattttgttt
    11221 tctagacctc gttttgctcc cacatacatg aattcagcat tcggttcttg agtctttaca
    11281 taattgacaa aggcaagagc tggataaata tgtccccctg ttcctccgcc tgtaaccaaa
    11341 attttcattt tttatcgctc gcttctactt tttctatttt aatcggttca cagcttccat
    11401 gaatctgttt cctctagttt caaaatttgg gtattgatcc cagctggcat tcgctggtga
    11461 taataaaact acatcaccat ctgtactgtt ttctaatgct aaaggaactg cttgttctac
    11521 agaatcggct gttactattg tctcgatgcc tgcttttttt ccagcatcca tcaacttttc
    11581 ttttgtttgg ccaaatacga ccattgcttt gattcctttt aatgaaggaa ttaattcatc
    11641 aaatgtattt cctctgtcta atccgccagc caataacacg accttagacg tttcaaatcc
    11701 acttagagcc attttagtcg ctaaaatatt ggttgctttt gaatcattat aaaattttct
    11761 gccttggatt tctcctacat attgcgtacg atgtggtacg ccatggaaaa aatgcaacgt
    11821 ttctcgtata gcttcatttg atataccata caattttgct acagaaatag cagctagtgc
    11881 attttctaca ttatgcttac ctggtacgcc aagttcagat atttccatta ttttttcttt
    11941 tttatagtat attgagtaat cgtcagcata cacaccgtct tccaacactt cttttgtcga
    12001 gaaaggcagt acgcgtgctt ttgttctttg cgctaattct tgtagttctg actgattcca
    12061 gttaaggatc agataatctt tttctgtcat gtttttttga aggttccatt tagctttgac
    12121 atattctttt cttgtaccat gataatcaat atgtgcttca tagatattgg taatcaccgc
    12181 tatatgaggg cggaaatctg tgattcccat gagttggaag ctagacaatt ccatcacaat
    12241 tttatcttct gattttgctt cttgcgctac accacttgct ggatacccaa tattacccgc
    12301 taaacgtgca attccttgat ctgctcctgc attcagtagt agtcctgtca tcgttgttgt
    12361 cgttgttttt ccgttcgttc ctgtaatccc aataattggg cattccgcta cttcgtaagc
    12421 tagctcaact tccgtaatta ccggaatacc catttcttgg gcttttgcta ctaaaggatg
    12481 actgtaggga atccctggat ttttgaccat caatgaaaat tcttcatcca acaattcgat
    12541 tggatgacta cctgttatta cttttatccc taaagaaagc agctcttgtg cttccggatt
    12601 ttcgtcaaaa ggttttccat cattaacggt gacaagtgcg cccaattcat gtaataattt
    12661 ggccgcactt actccgcttt ttgcgagtcc taagacaagc actttcttat tttcatatgt
    12721 tgatattttt ttcatttccc gtcatctcct accaaacgat ccataatgtg atcaacgaag
    12781 tcacaatgct gatcaaccaa aaaactgtat caatcttcca ctccgaccat ccgcacattt
    12841 caaagtgatg atgaattgga gacattttaa aaattcgttt tcctgtaagc ttaaaggaag
    12901 taacttgaag catcacactt gctgtttcca taacatagat caaaccaatc aaaagcaatg
    12961 tccattcttg gtttagcatt atagaaattg ccgctaaaag tccgcccaac gctaatgaac
    13021 cgacatctcc cataaaaatc ttggcaggtt ttctgttgta gacgaagaaa cccagtaaac
    13081 ctcctaaaac acttaagcaa atgattaata catcatactg atgctgatgc caagcaatta
    13141 gaccgtaagc tgcaaaagag atacttccta agccagcaac taatccatcg atgccatctg
    13201 tcagatttac tgcatttgaa aatccaacaa gccagaagag agcgaaaata ccataaatga
    13261 ttcctaaagg tagatctagt ccgaagaaat tcaatacacc tggataacct tcagaacggt
    13321 aaacaagata aaagatgatt ccccctagaa tctgtccaat caatttctgc ttcgagttca
    13381 gtcccatgtt tcttttcttg aatattttta taaaatcatc aagaaagccg attactccgt
    13441 ataacgctaa tacaaaaagc aagatgaaaa gagtaggagt tagctgtttt tgccaagctc
    13501 caacccaaat tcctgtcaaa atagagccga tcagaaacac taagccgccc attgtaggag
    13561 ttccggcttt gctattgtgc cactttggtc cctcctcacg aatcgcctga ccttgctttt
    13621 tcatttgaaa ataaccaata aatagcggca ttgcggcaat tgtcattgca caactgctga
    13681 caatgggaat caatgcttgt gtccattcca tgctcttttc tctcctattt ctcttcttat
    13741 tcttctaaag taaaactaag tttgtcttca gtgatttttt catatggagc aagttcttgt
    13801 ttgacacaat aaccatctcc atcaaaacta acttcaatac cgaggatttt ccccagcttc
    13861 atgatatctg cttttgacca tcctgttacg tcaggcatca gcttatcacc accagtatat
    13921 aggatcagtt tttctccaga gatcaactga tcaccgttgg cagtagattg tttttgtact
    13981 tttttaccat taccgatcac tacaggttgc agtccacttt tttgagcatc agctgctgca
    14041 acgtccgctt caagattacg ataatcggct acggatactt tttctgtttt tgtttctgta
    14101 tctgaatctt cttcagtttc tttaaaatcc attgcccgtt tcatcaatgg atttgcgatt
    14161 ttagccaatg ccattcgatc atatgtctta gggtgtttca ttgttagata caagacatag
    14221 tctggatctt cagaaggaac catctccaca atcgaataca ggtaggctgt atctcctgtt
    14281 tgatatcccc ctgtatcaga agcgatttgg gctgttccgg tttttgccga tacattgtat
    14341 ccaggtacac tatatacacc ataagcacta ccgtagtttt cgctttcgac aacatctctc
    14401 atatattcac ggactttttc tgttgtttct tttgaaaaag gttgccctag aacttctgtt
    14461 tgagttgttc gctcttctcc agtttgggga tccaccactt tgctgatata tcgaggttga
    14521 atcattgtac cgttattcgc aatagaagtg aaagctttca tcatttggaa attcgtcaca
    14581 cctaccgctt gtccataagc actcattgct cggtcgacaa tatttaatgt aggtaaagca
    14641 ccgtttactt catcatctaa tccagaatga gtactttgtc caaatcccag tttttgtaaa
    14701 tagttatacc atcgtccgcc taatcgctgt tccagtatga ccatcccgac gttacttgac
    14761 catgaaagag cttgacgcat ggtcagcaca cctttttcac caaagtcatg gtcattgatc
    14821 gtagcatctt ctacttggat ttttccagat tggaaggttt cattctcgtt gaattctcct
    14881 tcttcgatag cagctgcagt agtgaaaact ttcatggttg atcctggttc ataactatct
    14941 tgtaccaaga aattacgcca gatagcatct tttccagtca atccttccat tgtttcagga
    15001 ttgaaagtag gtcgttgtcc cattgccaag atttctccgg tttttgcttt catcaggact
    15061 gctgtcaatt cttctggctg atattcttca tttacttgat ccatcagtgt ttctaaataa
    15121 ctttgtagtc tgctgtctaa agtcgtgtag atatcttgtc cgtcgacagc tttttcttct
    15181 tctgccactg tacctggcag aggattttga aaattatctt tttgataaat aatcttacca
    15241 tctttaccgc tcaagatatc attgtaagca gactccaaac ctagtttacc aactaagcca
    15301 ttctcatctt tgtctggaac tgcgtatcca ataaaatgag aagaaaagac gccatttgga
    15361 tacatccgag cctgatggtc gacaaaataa agaccagcga caccttcttt tttcatgtcc
    15421 gcttcgatgt tttgcttggt ttcttgggag atatttttcc catagctacc aaattcaact
    15481 tgatacagct ctttttttgc gccgtcctct aaaatattca gtgcatcttt tttgtctata
    15541 gaaagattct tatgcaggat ttctgctatt ttatcaaaat tcttttcttc aacatacagt
    15601 tttttatccc cagaggtata tgtttttgag agtatcgctt taattgaata agaggaggca
    15661 tcttctgcga gcgccacacc gttacgatca taaatcgttc ctctttttgc ttttactact
    15721 tcacttcctt ggtacaactg ttttgttttt tcagctaatg aggtaccagc aacatgacct
    15781 ccaataacga tatacgagaa acggacggca aataaaaaga acattccaat actcgtagca
    15841 aataaaatga tacctacttt tttgcgattg ttcatcggat ttagattttt cttttccata
    15901 aaccgccgaa ttttattttt aagactcatt tacttcactt tccttaaatt gtcgtcatta
    15961 atagacaatc ctttttcttc tgcgatttgt ttaatccgtt cagtacgaga aagttcgttt
    16021 ttttcttgtt ctaaactcgt tttttcttgc tttttatcat tgatttctgt ttgtattgac
    16081 gtgatttctt tttcagctcc gctgatattt gtgcgcagca taactgttaa aacacataaa
    16141 gcaacaatag caatcgccac gcttgccaca gcaatttttt ccaaagcaga aatctgcttt
    16201 aatcgttttg ccggtgattg tggaagaatg atttcttgat tcgaagaaga atcaggcatt
    16261 tgttgttccg gctgatccac caattcaggt aaatcatatt cgtaatcttg cattttcttt
    16321 aattctgcca tcattctact cctctttcat tcttattttt tctgcaatac gtaactttgc
    16381 actgcgtgat cgattgtttt ctgttagctc ttcctctgaa ggaaggattg gtttacgagt
    16441 aatgactttc agttctggtt ggaattcgtc aggtatgaca ggaagtcccg gtggtagatc
    16501 ttgtaccgta ctatattctt taaacatact tttgacaatt ctgtcttcca atgaatggaa
    16561 tgtgattacg ctgattcttc cattgacttt caataaccgg attgcctgtt ctaaagaggc
    16621 ctcttcggca ccgagttcgt catttactgc aatccgcaca gcttgaaaaa ttctttttgc
    16681 aggatgcccg ccttttcttc ttgcgggagc tgggatgact tgtttaatga tttcgacaag
    16741 ctcaccagtt gtttggattg gttttgtttt tcgtactcgt tcgatttcac gagcaatttg
    16801 tttggaaaat ttctcctcgc catagcggaa gaaaatcttt actaattcat ggtaggaata
    16861 ttcattaatt acgtgataag cggataaagg agctgactga tccatgcgca tatccaacgg
    16921 tgcatcttga tgataactaa atccacgttc tgcttcatct agctgaggag aggaaacccc
    16981 tagatcatac aaaatgccgt ctaccttatc tacatgctca ctcaagcgtt cttctaattc
    17041 tctgaagttc gctttgataa atgtgaccat accttgttcg acataagaag ctaaacgcag
    17101 cttggcgtga tcgattgctt tttgatcttg gtcaaacgca tacagatgcc ctttagaact
    17161 tagttggctt aataaatatt cactatgtcc ggcaccgccg agtgtacagt ctacataaac
    17221 gccatccggt ttgatgttca atccatctac cgtttctttc aacatgactg tatagtgttg
    17281 aaatgtttca gtcattagtc agcctcttcc tattcttata gtccgaaatc aatcatactt
    17341 tcagcaattt cgtcaaagtt ctcttctgct gctgcagaaa aatcttgcca tcgtgtctca
    17401 tcccaaattt caattcggtt agatacaccg atgactacgc aaccttttgt caaagctgca
    17461 taatttctta acgtgctggg tatattgatt cgtccttgtt tatcaagttc gcattcagtg
    17521 gcagccgaat agaagaatcg cacaaaagta cgtgcgtctt ttttagccaa cggcatttcg
    17581 ttcagtttag cttccagatt ttcccattcg ctcatcggat agccgaaaag acagccatcc
    17641 aatccgcgtg tcagaacaaa cttttcgcca agttcttcac gaagttttga gggcacgatt
    17701 aaacgtcctt ttgcatcaat attatgttgg aattcaccca tcaacattga aagcccccca
    17761 ttcctccact aactaaaatt agtctaccac aatcccccac tttctaccac cttttctggg
    17821 aaatccggga acaacttttt ttattcataa acattttttt aaaaatcaag ctgcaaaaag
    17881 ccttttaaaa gactttttac agcttttcta tttttttcat aggtaagaaa gtatgttcac
    17941 aatgatcaag acagcgtaca agacgattgt caacagaaaa accaaacgcc aagtcatctt
    18001 gaaataacgc ggataaataa tttcttcata ataatatgct tggaaaacag caatacaaat
    18061 tcccattagc aaaacagaaa taaagaaaaa cggtaagata gaaattgtcc ctgtatttct
    18121 agacaatgca tgcataccgc cccacaaaaa aggaatcgcc aaatcagggg cttttatttt
    18181 aaaacgacga tttaacgaaa ataattttac aagaaattgg caaccaaaca aaacgattac
    18241 cgggtaaata taccaaaaca agataagtgg tgtgaatgtg cccatgaacg taaactcctt
    18301 ttttatcgtt gcttcaagta tactgtatat actttacaca actgtttcat tttatttaaa
    18361 tttatgcagt tatattctat atcttacaaa cacattccat gtattccaat tccctatccg
    18421 ttataataaa agaaacaggc aacaggagga aaatcatgat caaatatttt acattagata
    18481 atgagaaact gcgtaatacc ggaaaggaaa ctgaccaaac gatctggtat tgtgtagaac
    18541 gtcctaatca agaagagatt catcagctga cacatcaatt tgaaattccg tcagattaca
    18601 tcacttccat attggacgat gcagaaaatt cccgtacaga agaattcaat caatcaaagt
    18661 tgacaaagcc agcgttatta ttgttacaat atccttttgc taaaacaagt cctagcggtt
    18721 attttcaagt ggatacatac ccgctctcta ttattttgac gcccaaaaag aagcttatca
    18781 caattacgaa tcatgaacca ctgtttttaa agaaagtctt tagccagacc tttccaaaaa
    18841 gtgatatttc gcctaatctc aatatcttct ttcaattcat ttggcaggtc acactctctt
    18901 acaacagtta tttagctacg cttcaatctc aatgcgatac tttggaagcc aaacttcaag
    18961 tatccactga aaacaaacaa ctttatcaaa tcatggatat ccaaaaaagc ctcgtttatt
    19021 tcagagaagc gacttctgct aatttagatg cattgaaaga actagcgcaa aataaacttt
    19081 tccaagaaaa tcatgctttg aaaaatcatt tacgtgatgt attggtggaa acagagcagg
    19141 caatgactac ttctcgcatt caattaaaac tagtagaaca aatgaatcag actttttcag
    19201 caatcgtatc gaataatctg aataatatta tgaaaatttt aacttcttta acgattattt
    19261 taactatacc gacaatcgtc ggcagtatct atggaatgaa cattaagtta cctattgctg
    19321 aaagggatga tgcctttata tggctagttc tattaacatt atttatttgt atcgtaacta
    19381 cctattattt aaaaaaaggg aagtttttat aaaaaagccc tcttgtggaa atggaaaaaa
    19441 gattgtacac ttgaatcaac taattaatga cgaggttgaa aacaatgcgt gaaaaaaaac
    19501 aaacaagtac ttttgctaaa gtaacaaaat ttgtcatttg gacaatgctg attttaacga
    19561 ttggatctgt tgtattgact gctgtaatga gtgtcatgta aaaataaaat atagctatca
    19621 gctgctgaaa aggacctgaa ccactgcgag gtggctcagg tcctttttgc gagtttcaat
    19681 agttcttttg catgttcgag tgtgagtttt gtaatctcac ttccagccag catacgtgca
    19741 atttcttctt ctctttcttt tgttttcaac tctgaaacag aagtttgcgt acgtccatca
    19801 atcactgctt tcacaatata atattgatag tcggcgactg cagcaacttg aggcagatga
    19861 gtgatgcaca aaacttgaga gtatttcgat atttgagaaa ttttatctgc gatggcctgt
    19921 gcaactcttc cgctcacacc tgtatccact tcatcaaaaa taatacttgt cacaccttgc
    19981 tcagaagaaa aaatagtttt caaagcaagc aacatccgag acagttcgcc accagaagcg
    20041 actttcacca aaggcttcag tggctctcct ggattggtcg taatgtaaaa ttcgatttgg
    20101 tcaaatccat catgattcaa ttgtctattt tcttctttta agaaacgtac ctcaaactca
    20161 gtgttttcca tatacagact tttcaattca tgcagaatgg atttttccaa ttcttttgcg
    20221 atttttttac gcgcttcatg aagatctgcg gcttgttgct gaagtagaag ctctttttgc
    20281 tctagttcct ctttcatttt gtccaattga ccttctgaaa aatcagaaga agctagctct
    20341 tcggtgattt cgtcatagta agccaatatt gactcaatgg attcgccgta tttacgtttc
    20401 aactgtcgga ttaattctaa acgttgcgta acttcttcta aacgactctc atctaactcc
    20461 aataaatcaa tctgacgact catatcgccg attgcatctt gcagcaaata gtaagcactt
    20521 tggatatttt cataaattgc ttcatactcc acatctagat gggcgatccc ttgcagttct
    20581 gaaacggcca agccaacacc atctacactg ttttgttcat tattcgtcag tgctccgtaa
    20641 cctgcagcta atccatcagc aattttttga taattgctaa gtttttctct ttcttctttt
    20701 aatttctctt cttctccgac ttgcaaatca gcagcagcta tttcttcttg ctggaaatgg
    20761 agcatatcta ttctctgaac ataggatttt tcattttgct gcacttttcg tacccttttt
    20821 tccagctcac gataagagat atattcttgc tgataactct ttttcttctg ttggaaagct
    20881 acatctccaa aacgatccaa taaagcaaga tgtgattcag gctggagcag ttcctgatgt
    20941 tcgttttgtc cttggatatc tactaaataa ctgccgattc ttctcagatt agctaatgtg
    21001 ataatatgac cattcactcg acaaacattt tttccagtta aggacatatc ccgtcgaaca
    21061 atcagattgt cctcatctgt ttcgatccct aattcaacca tcagttcaga gaatccttct
    21121 tgttttggta gttcaaacaa cccttcaagc acacattttt ctgctccttg acgtatataa
    21181 tcgcttgaac ctcttcctcc tgctaacaga cctaaagcat cgataataat ggatttaccc
    21241 gcaccagtct ctccggtcag cgcggtcatt ccttcgtgaa aagaaagccg caattccgga
    21301 ataatcgcaa agttagtaat actgatttct tgtagcatca cattcgctcc tcaacttaaa
    21361 ggtaatgcag gaagtctttc ttcaataaaa ctgcctggtc ttcagttcgt gtaatcatca
    21421 aaacattatc gtcatcattg ataatcgaaa ataattcttt cttgtaacgt ttatcaatca
    21481 aatttgctgc tgccgaagca tttcccggca atgttttcaa aataacaaat ttttccattt
    21541 ggtcaactgc cacgaaagcg tcttttaata gtttttccaa tttagtattt acatcttcgt
    21601 ttgtttcagc tggtatgcta taacggtagc cgccatctga agccggtacc ttgatcagtt
    21661 tcagttcctt tatatctctg gaaatcgtag cttgagtaac agatattcct ttgcttttca
    21721 ggatttcaac aaattcttcc tgctttcgga tatcttgctc actcaacaat cgagtaatca
    21781 aacgatgccg atcttttttt ctcattacgt cttcgctcct tagcatattc ttcttaatca
    21841 atgatagctt atttccttgg tataataaag aagaatccgc atacttctca cttgattttc
    21901 acagttacaa tttatttatg gtcaaactgt tcgtgtgcat ttgaaaccac ttctgccata
    21961 ttaatagctg aattgattgt tccgctttcg ggtactttcc tcaagtgagc caagaactcg
    22021 atatttcctt ctccccccgt gatcggagaa taacttaatt ccagcacatc atatccacct
    22081 tgtgccgcaa atgacaaaat gtcttctaaa actttttgat gggtctgagg gtcacgaaca
    22141 attccgtttt tccctacagc ttctctgcct gcttcgaatt ggggtttgat taaagcaatc
    22201 acttctccct cttcttttaa aatagcatgt aatggaggca gcatcagttt tagtgaaata
    22261 aaagaaacat cgataacagc aacttctgga attcctaagg taaagtcttc gggttttgca
    22321 taacgaaaat tcactctttc cattacgacg actctaggat cttgtcttat tttccaagct
    22381 agctgattgt agccaacgtc taaagcataa ctcatttttg cgccattttg caaagctgca
    22441 tcagtaaatc cgccagtgga tgcaccaata tctaacaatg tttttccttc aacagataga
    22501 tcaaatgctt tgagtgcttt ttctaatttt aatccccctc gagaaacata aggaagtttt
    22561 ttacctttga ctcttaattc actactcact ggaatttttt ctcccggttt atcaaaccgc
    22621 tcgttttttt cgttgtaaat caaaccagcc atcactgaac gtttggcttg ttctcttgtt
    22681 tcaaataatc cttgtttgac cgctaataca tctacgcgtt ctttttccat tctttccctc
    22741 atttctccac atcaaattgc ttgatcatct ggcgcatcag cccgccatca aaagaaaaaa
    22801 cgtcctcttc taatttgtct atgatcttgt tggcggataa cagctcaatc tccaacgcct
    22861 cacgtgtctt ttctaggcct aataaacgcg ggtaagtatt tttttccata cgttcatctt
    22921 taccagctgt tttccctaat gttttcgttg taccaatcac atcgagtaga tcatctctta
    22981 tctgaaaagc taaacctaaa tgttcggcta atcgttgaag tagcaataag atttcttcag
    23041 gttgcttcgc taatatgcca ccagccagca aggcataacg agtcaaatgc cctgtctttc
    23101 tttcatgaat aaatgctaat tcttctaaag aaagcttttt cgattcgccc tcgatatctg
    23161 cagcttgccc agcaaccata ccttgtgatc ccgcacaaac agctaattgc tgaagcagca
    23221 gaagcttagg agaattacct aagtgtgcca tactgattag ctggaatgct cctgtaagca
    23281 aaccatctcc ggccaatata gctaatgctt cgccgaatac tttatgattg gtaggttttc
    23341 ctctacgaag atcatcattg tccatcgcag gcaaatcatc gtggatcaag gaatacgtat
    23401 gcaccatttc taaagcagct gctacttgat agacgggaac atcaataggc tcatcaaagg
    23461 aagcaacggc cgttaaaagt aataagggac gtattctttt cccgcctgct ttcactgaat
    23521 aactcatcgc atcatagagt cctctatccg ttgtatgctc atctaaaaaa gacaagattt
    23581 ctttttctac agcaggtaga tgaatagctg aaaagtcagt taatctcatc tcagctttcc
    23641 tcgtttccgt caaaatcaac ttcttcattt gattctgtca tcattttcgt caaggtctct
    23701 tctgctttag ctaatgtatc ttggcaatgt ttgcttaatt ccattcctcg tttaaacgaa
    23761 tctagagctg attctaatgg aacgtctcct tgttctaact gcatgacaat tttttctaat
    23821 tcttgcagtg actcttcaaa agttggattt tccatatttt tattctcctt tgtctatctc
    23881 ttgtacttgt cctttaacga ttccatcttg ataatggatc gtcagcaaat ccccttgatg
    23941 aatctcggtg acacttttta ctacttgatc atttaacgtc gtatagctgt agcctcttcc
    24001 catgattttc aatggactca acaaatcaag agattgaaca gcttgagcaa aacgctgctg
    24061 ctgttgtttc atatattgtt ggattttctc ttctaagcgt ttttctagat aagcagtttc
    24121 ttggcgagct gctttaactc gataaagcgg agccacttgt tccaattggt ggcttaatgc
    24181 gatcgtttgt ttttctttcg catgataaat ttgctgtgta gcttgatata atcgctgttt
    24241 caactggtct aatttgatgg tctgaccttc atacaatctt tcaggctgcc gaaatacata
    24301 ggaacgtcga gcacgctcga accgttcttg tttgcgctgg atctggcgta caaacgcctg
    24361 ctgcaatcta gcttgccgtt cttgaatgcg catcaactct tctgacaaaa caggaacagc
    24421 taattctgca gctgctgttg gagtggcagc ccgaacatct gctactaaat cagcgatcgt
    24481 ggtatctgtt tcatgtccta cagatgaaat caccggcgta ttagcttgat aaatcgcccg
    24541 agcaacccgc tcttcattaa acggccataa atcctcaatc gaaccacccc cacgaccgat
    24601 gatcattgta tcgaaattgc ccatctcttc tactctctgg atattcctga caacattgtc
    24661 cgctgcttgg tttccttgta caactgttgg gaacaaaacc aattggacaa ttggatatct
    24721 tcttttcacc gtcgtgatga tatcgcggat aaccgctccg cttggactgg taaggaccgc
    24781 gattcgcttt gggaaacgtg gcagcatttt tttaggccct tgaaaaaggc cttccttcat
    24841 cagcttttct tttcgttctt ccaattcttg atataaagca cctattccat caggctccat
    24901 gtgctctaca tagatttgat aagaacctcc cgcttcatat aatgaaatac gcccaacgac
    24961 aaggactttc attccttctt ttggctggaa tcgtaatttt tggaatgctc ctttgaacat
    25021 aatagcagat atttttgcat ggtcatcttt taagctgaaa tattgatggg cattggcacg
    25081 taaacggaaa ttcgatattt cgcctgttaa atatacgcgt tctaaataag gatcggcatc
    25141 aaattttctt ttcaaatact tcgttaatgt agtgacagtt aaatattctt gtgccatcta
    25201 ttcacctgct ccaaaagctt cggcagattt tacggtttga tacatcagca tcgtgatagt
    25261 cattggccct acaccctttg gcacaggagt gatccagcct gcaataggct ccacttcatc
    25321 aaatttcaca tcaccgatca gcttaccgtt agcatcacga ttcatcccta catctatcac
    25381 tacagcacct ggttttacga attcttttgt gacaaaatgt ccacgtccta ttgctacgac
    25441 taaaatatct gcttcttgtg ccagttcagc cagattttcg gttttagaat gagcaacagt
    25501 aacagttgca tctgccatca agagtagctg agccatcggt ttaccgacga tattgcttcg
    25561 tccgataact actgctcgtt tacccgccgg atcgatagaa taagcttcaa acattttcat
    25621 gatgccgtat ggagtacaag gaatcttgtc cggtttaccc gcaaggagtc tgcctaaatt
    25681 catcggatga aaaccgtcta cgtctttttc agggcggata gctaacagga tcttttcttc
    25741 atctatatgc tttggcaatg gtaattggac caatatacca tgaaaactag gatcttgatt
    25801 gtatttttca atttctgcaa gtagttcgtc ttcagaaatc gtctcaggat aacgctctac
    25861 caatgaatgg attccgattt cttttgctac acgttcttta tttcttacat agatttggct
    25921 ggctggattt tcccctacca ataaaacgac taatccggga tggataccat tatctttcaa
    25981 tttttggatt ttttcagcaa tctctgcctg cattttttca gctaattctt ttccgttaat
    26041 caattctgcc atctaaccac tcccagatat tttttgtgtt ttcattctac catagaattt
    26101 tcccgattta taacaggttt atgatgattc atagaaaaaa gctaggactt acgccccagc
    26161 ttctaattct ttcaatacat tggataaaac accgttgacg aatttacgtg aacggtcatc
    26221 actaaatgtt tttgccaact cgattgcttc atttaaagca acagccgctg gtacatctgt
    26281 cacataagtc atttcaaaaa tcgcgatacg caagatcacc aaatccattt ttgagatccg
    26341 attgatgtgc caattgtttt ttaaatgttt ttcaatcact ttatccaatt catctttttt
    26401 tgcacagaca ccgccaacta gcgtatcaag ataaacagga acaaactctg attcatcttc
    26461 gttgatcatg tcgcggtggt ctaattcgat tgcgttgaaa atcgcatctt cttttgttag
    26521 gtctgcattg aagtctaatg gaaataaagc ctgcaatgcc atttcccgaa tctcgtgtct
    26581 tgataattct ttattcattc tcttcctctt cgtcttcaaa aagttcttcg attgttggtt
    26641 gttctaattt ttcaggaaca acagctacta catggatatt gacttcagat aaatcgatat
    26701 ccgtcatgaa aaggacttgc tggctgaccc gttcctgcat ttccattgct actttaggaa
    26761 ctgaaacacc atattctaga taacagtaaa gatctacttt caagccatct tcctcgtttg
    26821 ctaagtaaac accttttccg tgagctgcac gtcctaataa ttcagtgaca ttgctggcaa
    26881 aagttccgcg cattccgtat acgccttcta ctttagaagc tgcaatcccg ataattactt
    26941 cgatgacttc aggagcgatc acgatctctc ctaaatcttg gtttgcgttt aaaatcaaat
    27001 ttttttcatc tgccatttat ttattcctcc ctttgcctaa gcacgagata catccgaatt
    27061 ctctatgatg cggcatagtg atcacgtggt aagaaaactg ccgccgatca gctgcaaact
    27121 gctatgaatt ggagaaaaca cccaacttta ttatctctta cagcttaaaa gttcttctat
    27181 agtgtatcat taaatcgagt gattttctat aacaaagtct gtgaacactg atttttacgc
    27241 ttacttcttc tattgatgaa actataagat gatcaattct tttggtgaac gagtcaacac
    27301 acgatttcca tcttctgtga tcaacaagtc atcctcaata cgtactccac cgattcctgg
    27361 taagtagatc cctggttcat cagtgatcac atttcctggt acaaattgct tttctgcacg
    27421 agaagaaacg tttggtcctt cgtggatctc taaaccgatc ccatgtcctg tagaatgtcc
    27481 aaatgcttcc ccgtaaccat gttttgaaat ataatctcta gcaactgcat ctaattggac
    27541 accagtcatt cctggttttg cagcatcaat aactgctaat tgagcatcta agactatttg
    27601 ataaatttct tttaattttt ccccaggatc accaatagcg aatgtacgtg tcatatcgga
    27661 aacgtaccct tcgtaatagc aaccaaagtc caatgtgatc aagtcacctt gttcgatgat
    27721 ttttttgctt gctacaccat gcggcatagc cgaacgcaat ccgctagcaa cgatcgtatc
    27781 aaaagaaaca cctgaagcgc caagagaacg catataaaag tctagttgat ttgcgacttc
    27841 aatctcagtc atgcccggtt ggatcatttt caggatgtga tcatatgcca tatccgcaat
    27901 atgacatgct ttttcgataa tagcgatttc ttcttcatct ttcacttctc tgagttcttc
    27961 gatcatctgt gaaacaggta ctaattccgc atcgatgatt tcttctaaaa ccgaatattc
    28021 taaaaatgag actgttgttt cctcaaaagc taatgagtgc agttcttctt tgcgtactaa
    28081 gtttgctacc tcatcaaaaa taggtccgac atttttaata atttcaaacc cttgagcctg
    28141 cgctgccgcc tgctcggtat aacgaaaatc agtaataaaa aatgcttttt ccaaagtaat
    28201 aacagctaat cctgtcgtac ctgtaaagtt cgttaagtaa cgaagattgt acggactggt
    28261 aaccaaaaat gaatccaaat tttcttcttg catttttttg cgcaattttt ctacacgtag
    28321 catcataatg aaactccctc tttctttgca cgcttttttc cattatagca aacttttctt
    28381 cgtacacgac agttaaataa aaatttccgg aaaacttctt tttattttaa ataaataaag
    28441 ccaaaaagga gtcacagtct cgctgcaact ccttttagtt tactattttg taaaaactta
    28501 tgatcgtttt ccaaagaaaa atgagacaac tgctaccaaa atcactgcgc ctaaaataga
    28561 tggaataagt gccatacctg cagcttttgg tccccattcg ccgaaaatcg cttgtccaat
    28621 acttgaaccg actagtccag caatgatatt cgttacacag cccatcgaat ttcctttact
    28681 ggtgatcgca cctgcaattg ctccgatcac ggctccaaca atcaatgtcc ataaccaact
    28741 cataatataa cctccttctt tgctaatcaa ttttagcata tgggatttaa aggggacaaa
    28801 taattggttt tgctatttca tattttcttc tgcaaagtaa aaaaataaag aaaacctcct
    28861 cttagaagtt ttctttattt tttacttgcg tttaaacata ctacgaatac cacgtttcac
    28921 tacatcaaag aaactcaatg attgtaccat tctatcttca tctacatatt ctctgatcgc
    28981 acgaagcact tttttaggtt ctttggaaga aaatgtgtat gtaccatttt ttttcgtttg
    29041 aatggcatat cgaggaatcc attttccctt tagcatgact gatgctagta cataatccac
    29101 ttcttcccat ggaatttgga tatatttgcg tctatctcgc gcatggtaaa actcaaaacc
    29161 attatcacct atcatgattt gcccgtaatc tgtcagaccc gtaaacgaag tagcatcaat
    29221 taccaagtct acttttgtat tgattgattg aaccattttc tccctccaga tatttgttta
    29281 tgaacagtat actatataaa aaaagctcgg gacaacgccc gagctatatt tttatggatt
    29341 ataaaagtcc gatgacgtgt ccaacaacac cgactacgaa taaaccaagg atgataacga
    29401 ttggagaaac ctttttctta agcaaccaca tacataacaa tgttaatcct aatgcagcta
    29461 aaccaggaat caattggtct aagttgtctt gtaatgttgt taccttttca ggagaaaggg
    29521 ctaagccatt attaacttgt tcaaatgcac tcttgattcc ttcaccacct gaaggcaatt
    29581 tatcccattc gatgtaagca cctttatcta attttacagt tgatacaact ggtaagaatt
    29641 tgattgatac ccatctttct acaagggcag ctaatacgaa catccctaaa attgaagcac
    29701 cttttgttac gtcttgtaac aatccgccag aaaggtcatc agtaatttta gaaccagctt
    29761 tgtagccaaa ttcttgtgta taccacatga atgcccaacg aataatgttc caacctaaga
    29821 agaagatgat tggtccaagg atatttcctc ccatagctaa tgaagcacct agtgcaccta
    29881 gcattggacg tactgtaaac cagaacactg ggtcaccaac accagctaaa ggacccatca
    29941 tacctacttt aaccccttga atcgctacat catcaacagg cgcgccattc gcacgttctt
    30001 cttccagagc caatgtaacc cctaaaattg gagaagcaat gtatgggtgt gtattaaaga
    30061 attccaagtg acgttttaat gctgcagaac gttcttcttt tgttttgtat aattttttga
    30121 ttgctggaat catagaaaat gcccagccac cgttttgcat acgttcgtag ttccatgaac
    30181 cttgaatgaa ggtagagcgc cacgcaacag ctaaacgatc tttttttgat aatttgattt
    30241 tttcttctgc cattttattt tcctccttca agattaatag tcattcaaaa tgtcgcctaa
    30301 agggtcacca gagttgcttc cgcctccatt atttgatgaa ccacccattt tagaaaggtt
    30361 caagtaaata agagctaatg caacacctaa agcacctaga gcaatcaatg ttaattgaga
    30421 aattgcagct acaacgaaac caataacgaa gaatggccat acttcttttg tagccatcat
    30481 gttgattacc aaagcgtaac ctacagctac gaccattccc ccaccgatag ccataccatc
    30541 tgttaaccat gctggcattg attcaaggaa tgattgcaca gtttgtgctg ggataaatag
    30601 caacgctgct gctgggaccg cgatacgaac cccttgtaaa catacagcaa tgatgtgcca
    30661 catttcaacc ttacggatgt ttccttcttc agcagccgca tccatcaaat gaacgattgg
    30721 aacagcgatt gtacgtacga tcattgttaa gaacaaccct gcaacagcca aaggaacggc
    30781 aatcgcgatc gcagaaggaa cacctttaac accttgtcca cctaaaacta aaataattgc
    30841 tgatgctact gatgctaatg cagcatctgg tgctacagcg gcaccgatat ttgcccaacc
    30901 taaagcgatc atctgaagcg taccgccaag tacgatacca gcttcgaggt taccggtaac
    30961 taaaccgatc aatgtacatg ccactagtgg ttgatgaaat tgaaattcat caagaatacc
    31021 ttccatacca gctagaaagg caactaatac gactaaaatt attgaaataa tagacatgat
    31081 aagcctccta taaattttaa tattatttat tacgcttttg ctaattcagc ttttgctttt
    31141 ttcaaaatat catccatgtt atcgcgtgaa tcattaggta ctttacgtac atcgaatttc
    31201 acaccttttt gttcaagctt ctcaaacgct tcaacgtctt cttgtcccat agacaatact
    31261 ttacttacaa cgactttacc gaccgaatgc gccattgacc caacatttaa ttctttgatg
    31321 tctacgccac cttcaacagc tgtcaatacg tcttcaggat tttcaaataa aagcaatgct
    31381 ttggtatttc caaaacgagg gtcttttgct acttcgatca ttttgctaat aggaataaca
    31441 tttgctttta cacctggagg agcagcttgt tcgattagtc tcttacgcag atcatccttt
    31501 gatactgcat ctgaaacaac gataatacgg tttggttgca ctgcttttgt ccaagctgtc
    31561 gcaacttgtc catgtaacag acgtgagtca acacgtgcta atacatattt gatttttccg
    31621 tctccgacca cagttccttc tggaagtgct cctttaggtt gtgcgtcttc aactgctgct
    31681 ttcggtgctt ctgccggttc taattcttcc ggttttactt ttacaccgtc ttttgctgtt
    31741 tcaatgatgt gtgctgcaat ttcatgagca gattccattg aaaagcgtga tgcgtaggct
    31801 tcaatcaaca ttggtaaatt caaaccgctg acgattgccc atttatcttt gtgctcttcg
    31861 aataatgtgt ttgattgatt gaacggtgtt ccgccccata aatcaactaa gaataacact
    31921 tcgtcttgat tatcaaaaga agcaaccgct tcttccaatt tggctctcaa gtcatctgga
    31981 ccttcacttg gtttcaaaat cacggctttg acgttttctt gttcgccaaa gatcattgaa
    32041 ccggattgta agattccttc agcaaattgt ccatgacttg ctaggataat ccctaccata
    32101 tttatacctc ctatttactt ttgttgtaac aaattcaaaa atattaataa ttgctaacca
    32161 ttacaacaga attatttgac cttttccaac aaccattctt gctacacctt tttgattttc
    32221 gctagtagtt ccatcaaaag gattttcctg tcagcaggta cttttcgaat ctctaactcc
    32281 acttcttgac tgttcaagta acgaaaggaa tcaatatctt gttgatttac tgaaacaaaa
    32341 ttggtaatca ttgatttgcc ttcagtaaaa ctcataccgc cgatattcaa cgacttcaac
    32401 tgtacaccgc agcggacaac cgcttcaaca tcttggggat tggtgaataa aagcatcact
    32461 tttgctcgtg ataataatcc actatgatag atttgaatca ttttctgcac ggttattaca
    32521 tttgctgtga tccccggcgg tgcagcttct tttagtagaa actttctcaa tttatcttga
    32581 gcgactacat cgctgatcac aatgatacgt tcaacacctg tttgcttcga ccaaactgtt
    32641 gcgacttgcc cgtggattaa acgatcatct atacgtgcca atcgaatatc catctgcaaa
    32701 actccttctt cctcactact tcacagaaac ccctatcatt tctgtgtgaa caaccacctc
    32761 ctagcacaca ctactataag caaaaagcgt gccaactttt ttacgataca ctaattgaat
    32821 ttaaaggatt cttaaccaat acacaaaaac gatacactat aaaagtgtat cgtttttgtg
    32881 tattcatggc acagtgtatt cttttttatc taactgatta tggactagac gtagcagata
    32941 atacatttct tctatcggca cttttacttt tatctgatct tccaacagaa caactgaggc
    33001 tgccaattgg ctataaagag gatcattcgt tgtatttgtc aattcgtttt cttccacagt
    33061 caatggttca ttccgaataa tacgttcgat cattcctgct gtgtgcatca ctaggttgat
    33121 tttgaatcca tattcttcat cgccgattcc cgtcgtctga gagatttgtg tagaaaattg
    33181 ccacattgga tcgattaact tactaccatt aataaagatg aagttatcag agataaactc
    33241 aacacacgta tttttagctg actcttcatc tagggagatg aattcagttt cctccaattc
    33301 tgattccatc agcaactgat ccaaaatgac ttcgatattt tgatcaataa aacgttctaa
    33361 aggaataaat ggggcttgga tattcaaatc agtaatccca gtagtagcta tgatctgata
    33421 ttctttttgg ataactggca gttcttcttt cacttccaca atcgacaaag ctagaacttc
    33481 taattctgtt tcttgacgct tgtttaccgc tcgttcgatc aattctttga ttctttgtgc
    33541 tgtaccttcg ccagatgcgc agatagccaa aacagctttt ttcttatgaa taggttttgc
    33601 ttcctgaaca gattctacag aagatatttc tgcgtaacct cgaaaatttt tcaatgattc
    33661 atgcaacata tctagatctg tccctaatac tgagaccttt cgtacagttt ccaaaactag
    33721 agaagttgtt accatatcaa cagtacgtac tggaattcca gtgtctcttt gaatttgcga
    33781 attaaacgac gcaagggacc ccatatctac taagagtatg accccgcttc cttcatctac
    33841 ttcttgtaca ttgcgttcaa ttcggtccaa tgctgttttc ggatccatat ccaaaggcat
    33901 gtctactgct cgtacgttct ctgcatctaa caactgttgg acaacttgga ccatactgct
    33961 tgctgtgctg tttccgtgag ctgcaacgac tacaccgatt ctgccgcttg cttgatttgc
    34021 ccgtaacgag acgagtagaa cggtcaagta atagtcttca ctttctggaa tctccacttg
    34081 aaaatacgtt tcgatataac tacgcacttc tttcgcaaca gcatattcat ctggaaaatc
    34141 aaaagccatc tcacggatgt tatcattcgt atgccgttct tctcctaagt tgattttttt
    34201 caaaaaagaa ctgatatgca agctcatagc ataaataaag ttttgctgaa actcatatgg
    34261 taaagatctt ttgaccatta cataaatctg attggtcact tctatgattt tcggatctac
    34321 aaattctgct aatttgctgt ctgcattaaa agaaaaacca tggtctttat agaaagactt
    34381 cagatggatg ttgatatctg tagaaatgaa atgattgatt gcttcttgat ccaaaccatc
    34441 cgacttcagt aaagctgctt tatctccgat gatatcatat agattataag gtaactcata
    34501 ggagtccgat tggattttca tgatcgtatc atttggtgct acagttatct ttggctccaa
    34561 tattttagaa agctctgcca ttgcactgcg gttgcttgct aattgaatca atccgctacg
    34621 aatcccttct gtcaaatcat cgattgtgat cgagatttcg ggtgattgca tatgatttag
    34681 aaatcctcgt gcacatacta actggatatt tgacttcaat tgtcctatat ttccataggt
    34741 cacactgccg atcaatgctt ttgctacatc ttctgtcaaa gaaatctttc gctgcgtgcg
    34801 gtttgcttcg tgggcaatca tgatttttac taaatcgact ttctcatttg caggacgttt
    34861 ttcaaaagcc ggaagtttga tattgattgg gatacgccga acaaaagttt ctaatagaga
    34921 agaacctggg tcttctgtgg tcgcacctac gatccgtaca tttgcttcat gggatttcgt
    34981 tgtttcccca agacgactgt aagttccatg atccataaag taaaagatca tttcttgacc
    35041 ttcaggcggc aaccggtgaa tctcatccaa aaacagcata ccgccatccg cttgatcgat
    35101 aatccccatt ttttcttcat ctgcgcctgt aaaagcacct tttgcgtacc caaataaatg
    35161 actcatcaaa agctcagggt tattggcata atcagcacaa ttgaaaacaa tcagttccgt
    35221 ttcctcatct attacatgat gcgcttttgc aaaatgatac atcgcgtgtg caaaataagt
    35281 ctttcctgat ccggtaggtc ctgtaatcaa acaatttaat cctcttggcg gatacaaaat
    35341 cgcagctttt gcctgttcca ctgaattttt catgctgccg ttagctccaa tgatttttgc
    35401 aaagatatcc ttagtatcta tttggagtgt atttttttct tcagagaccg gcgtttcttt
    35461 gtaactgact acatgttttg taactggctt tacgtgcgta acgagtgttt gatatatgta
    35521 acgtactggt cgtccgtctg tctttatcag ttttccttcg cgcactagct gattcagatc
    35581 tttactgcta tttgttcgct gaatccctaa cacttcagca atttcctgtg ttgttactcc
    35641 ttgatcatat tctgcctttg ttaaatgagc ggttttttct ttgacataca tataaattcg
    35701 gtcaatgcgt tttgccaatg gtatcgctcc ttttctatag actattgtac ttaggcttca
    35761 aaacataata ctatgtattt atttctgttt cgtcaactat ttacctgatt ttgttttgtt
    35821 gataacacgc ggatagatag aaatgttcaa tttaaaagaa cgaaaaatcc cgatacgatt
    35881 tttataatgg aaacaagaaa aagaacactg tttaccatat aattatttca ctgatagcaa
    35941 acaatatagc ttccatcaat tgaaaacgat agttatcaaa acatcctgat tccaaattgg
    36001 caaaaaaata ttaagtgtaa aaagacaaag taaacttcgt agcctccact acaactttta
    36061 tttttataaa caaaaaaggc tgtgacaaaa gtcttgtcac aacctcttta atcataagat
    36121 tcaattaagc ttcttgagct actggataaa cagacacttg ttttttgtta cggcctttac
    36181 gttcgaaacg tactacgcca tcaacttttg caaataaagt gtcgtctcca ccgataccca
    36241 cgtttacacc tggatagatt tttgtaccgc gttgacggta aagaattgat ccacctgtaa
    36301 ctgtttgtcc atcagcacgt ttagcaccta gacgttttga ttctgagtca cggccgttag
    36361 atgtagaacc gccacctttt ttgtgggcaa ataattgtag attcatagat aacaacatgg
    36421 tctgcacctc ctatttatca ttaattgttt taatttggat atattctgag ttttcagctt
    36481 ctatcgattg taatcctaat aaaagatttt ctaatagaat ctcagcaata ttgttttgtt
    36541 cttgtgtcat tcctgaagtg acttcggtat aaagataacc gccttcttct tcattcattt
    36601 caacaatcgg ctcaaaaccg gctaatgagg caatcccgtt gacagtgctg atcgctaacg
    36661 cagaaacacc cgcacaaacg atatcacttc cataaggacc agcatcagca tgcccggtca
    36721 aagtgaatga aacgatctga cctgcgtcat ttcgcttaaa tgttcctttg atcatatata
    36781 ggccttcttt ctcaaacgct attaagcgtt gatcgcgtcg ataaccactt ttgtatatgg
    36841 ttgacgatga ccttgtttac ggtgtgaatg ttttttaggt ttgtatttga aagtaacgac
    36901 tttcttttgt ttgccgtgtt tttcaacagt accttcaaca gttgcacctg caacagttgg
    36961 agcgcctact tttgtagatt ctccgcctac taagataact tcgtcaaaaa caactttttc
    37021 tcctgcttca acgtcaagtt tttcaacata gattgcttgt cccacttcaa cttttacttg
    37081 tttaccacca gttttaataa ttgcgtacat gctataaagc acctccttct gatatactta
    37141 gactcgccat ccgaagtgac agcatatgct gtgcttatta cttgttctga gcggttgtag
    37201 ctgtggagtc cacaattaca acagtactag cttaccagaa cagcaaccgt tagtcaacta
    37261 aatttaaaat caaaaagtaa aacaaaaatt caagcaatac ttctgctttt ctatcctcac
    37321 agttctacca aataaaatag tcgcattgtg tttcttttat tcttataata aaagaaaaag
    37381 gaggagcata aatggaatac ccaaaagaac tgatacaaga agcttcttta agaatgaacg
    37441 gccgtcctgt agaaggattt atcgctgggc aaggacctct tcatcctaaa ctgatgctcg
    37501 tgggagaagc cccagggaaa acagagattg atacgcatgt tccttttagc ggtcaagctg
    37561 gaaaagaatt gatgcaggca ctttcttcaa ctggcttaac acgagaagag gtgtatatca
    37621 ccagtgctgt aaggagccgc ccgtatcgcg tgactcatcg gatcaataaa cggacacaac
    37681 agtcagaaac tgtttatcca aatcggacac ctactcgttc cgaagtattt gcgcatgcac
    37741 caatattgga ttacgaactg atgaaggtcc agccaaaatt aattgcgaca cttggaaaca
    37801 ttggtcttca acgtttactt ggcaaagaat atacggttac caaaaatcat ggtcaactct
    37861 atacagggcc tgtcttacaa ttaacagaac aaaaggatgc ctacgaatgg tcagaaaaaa
    37921 actatcgaat tcttccactt tttcatccag cagctatttt ctacaatcgc tcccttgtac
    37981 ctgttatcca tcaagactgg caagtactag cttcactttt acagcaatat taaaaagaac
    38041 agacaagacc ataaaatctt cagtttacag tcttgtctgt tctttttcat tgttttaatg
    38101 gctaattgca gtatatttcg catgtaaagc tgtcaacgca tgagcaactg tttgatgttg
    38161 atgcgtgaat gtatgttccc acaagtgaga aagttgtttt tctacttctg gctgcaagga
    38221 agtatagata atcgtttgtc ctctttgctc tgctttttct tgaaattcat caaaaactgc
    38281 acgtgccgtc aaatcaatac ttggtacgcc tcgcatggag aaaatgacac catcgtgatg
    38341 atttagttct tccaatttat ttttgagttt ttctgaagac ataaaaaaca gtggaccact
    38401 aatatacact actgtccagt tattcagctt ttccgtttct ggcagcccca ttctttgcca
    38461 atcgatcgtt tctaatgaaa tcgtgatagc agcgcttttg acaacaaaaa atacacagcc
    38521 gccgatcacc ccgatcacga tagcaatact taaatcaaag ataactgtac aaatcatcgt
    38581 aaggataaac aatagaactg ctgaccaata tttattagta aatagctcct tgatcgtttc
    38641 ccattcattc atacgccaag ccgtcacaat caaaacacct gcaagtgctg gcattggaat
    38701 ctgtgacatc actggcgcaa aaacaagcat ggagagcagt aagacaactg catgtatcat
    38761 accagctaat ctagtttgtg caccagattt gatggccacg cttgtacggg cgatggccgc
    38821 agtagcagga atcccgccaa agaaaggaag caacagattt ccaattcctt gcgccattaa
    38881 ttcttgattg ctatctaagg gcttatttgc cattcggcca gccgaagcac cacatagcaa
    38941 gctttcaatc attcctaata aagcaatgct aatagctgga actgccactt gttgcaaggc
    39001 agaaaagcta aaatctccca tattcaaacg attactactg atcaatgttt gtggaatctt
    39061 acccacagtt gctactggta aatggaacaa tagcataact gctgttgcca aaatgatagc
    39121 cagcagcgaa ctaggcattc gtttcgccca tttttttgga tagataaaca tccctaaaat
    39181 gactagccct cccatgatca aagaggctgt gtttatcgga aatcctatcg tttgataact
    39241 acttagtttt tcaataacgt ttgtaccttc cgagtgaaca ccaaataaat tatcgatctg
    39301 acctaatgca ataatgatag caatacctga agtaaacccc gtaattacgg gtgctgggat
    39361 aaaagaagtc agtgttccta atcggaaaag tcctgctgca agtaaaaata gacccgctaa
    39421 aaaagtagca agcaatacac cttgcattcc ctttgttgcc gcaatagaca tcaagatagc
    39481 tgccatcgca cctgtcggac ctgatatttg ataaaaaccg cctgataaac cgccaataac
    39541 gataccggca atgatagccg taatcaaacc tgcggccgca tcagctccgc ttgatacccc
    39601 aaaagccaag gctaaaggca atgctacagc agctactgtg atcccagcca aaagatcttt
    39661 ctgaaatctt gaaagatcat aacctgaaaa ttcttttttt agtgaggaaa cataattttt
    39721 gaacacctat ctaactcctt tttcatgaaa ttttctaaaa taccattcat tagaatatca
    39781 tatttcatga gataaagtaa tatttttcac aaagtttcct gttagataaa ttcttatttt
    39841 ttctatattt ataagaaaat agaaagcaat gccaacagag ctagcccacc ttgtttgagt
    39901 aaaatccaac gatcgctagt caaactgcca tataatgcaa caaaaaaaat agatattaat
    39961 agcatacttg tgatttcttt agatgcggaa gaaagataag ctccataaag tagtcccact
    40021 cctaatagtc cattgtacac tccttggttc ttaaacaatg tttgaacaga ttttctgctt
    40081 aactcttctt tactcatttt aaaaacccta cttgtcgtgt cagaatcagt tctcaccgtt
    40141 tcgatataca tgatataaaa aaattcaatt gcaactagag tcactaaaat tctagatagt
    40201 atcgtcatat cttcttcctc ttttctgatg agttttattt ttttgctcct tataattgta
    40261 ctcgataagg atgttttttt attttatttg cctttgaaaa caaacaatat tggcacaaaa
    40321 aacaaagaaa aaccgttaga aacctcagtt tctaacggtt ttttagcgtc acaggaggga
    40381 ttcgaacccc cgaccgtccg cttagaaggc ggatgctcta tccagctgag ctactatgac
    40441 aagaatcagc ggaacatcaa taattataga taattaaatt gcttctgtca atacgaaata
    40501 gttatttaat tagcaaataa tgaattattt tggcccaact actaaatcaa tcttttaaaa
    40561 cgagacattc tccttcagct gttctaaggt tatcaattga aaacaggagc tacattgact
    40621 tcatgctact cctgtttatt tagatttcct gtatccttta attgacttac aaatatagaa
    40681 acttttacat caagttcgaa ttgagccctc gattcaatcg attatctaca ggctgccata
    40741 atcatgtatc acaactcgat tgtttctgat tcctttgtca ttaagatcac accatcacct
    40801 aatggcaaga ttgtcgatgt caaatcggga tgatccatca caacacgaag aaattcattt
    40861 aatttacgat ggatagctcg tttgccacgg ggaatctctt catctgggag caagatcgtt
    40921 cctccttgga agatgtcatc taccatcaaa acgccacctt ttcttaacaa acgtaagcat
    40981 tcaggtaaaa attcaatata ttttgatttt gcactatcca taaaaataaa gtcatacgga
    41041 ccagacaact ccggcaagat atctgctgct tgaccttcaa gcaaagtcac tttatctgtc
    41101 agtcctagac gttcatagtt ttttttagct ttctcgatca tgacatcgaa acgatcaatt
    41161 gtcgtaacat gagcgttttc tccgattgtc gtggccatga cacttgatga aaaaccgata
    41221 gctgctccga tctccaagat tcgttcaggc tgaagctgtc caagtaaaaa tttcaaaaat
    41281 acaactgttt catggggaat aactggtact tctgctacat gagcttcttc ttccagtttg
    41341 cctagctcac ccggtaattt tttttgctgg gtacgcataa aatccaatag ttcttctttg
    41401 actacaggac gatgcatcat ttcattgcgc atactactcc tcctcttcac acattatacg
    41461 aacaactgac taaatctaca agctgcatga gtgttttctc gtgtttcatg ttcagaagag
    41521 tcgttggatc tctggaatac atttatgttt ttaacattct tacaaaaatt tctgtgttcg
    41581 tctccgcttc tttttcaact ctacaaaggt attccatgct gtgattcctg ttaggcaaaa
    41641 agcaatcgta aagaataacc cctgaatcca tgcaaatgcc gtttttgaaa ggactagtcc
    41701 aatatatgga atctgaaaga tgggactacc gataatattt ctctttttta ctactccagg
    41761 ctcaatataa gtctttgtat cagcttttgt ataaaaatct tcgtggttgt tttcaacggc
    41821 tattacgcga cgcgtcgaga ttttttttcc tgaattttca taatatgtaa taatatctcc
    41881 gactaaaatc tcatttgtat ttttcggtcg tacatatatc agatttcctt gtgcataata
    41941 tggactcatt tctttgtcct caataatata aggagtcaaa ccaaaaattc ggggaaccaa
    42001 gacgacgaga agaataagaa ttaaaaatat aatcaacgtt atagaactta taaaagttat
    42061 ttttttgtaa tcaggtatcg ttttcaactt ctttctttat tcaatccttc ttaatcatat
    42121 aataacgata
//
LOCUS       DACIRY010000008        41205 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.008, whole genome shotgun sequence.
ACCESSION   DACIRY010000008 DACIRY010000000
VERSION     DACIRY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.008"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(26..328)
                     /locus_tag="IYE22_000344"
     CDS             complement(26..328)
                     /locus_tag="IYE22_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9505585.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IYE22_000345"
     CDS             complement(353..2446)
                     /locus_tag="IYE22_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ9505586.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2937..3701)
                     /locus_tag="IYE22_000346"
     CDS             complement(2937..3701)
                     /locus_tag="IYE22_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ9505587.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3812..4681)
                     /gene="mdcB"
                     /locus_tag="IYE22_000347"
     CDS             complement(3812..4681)
                     /gene="mdcB"
                     /locus_tag="IYE22_000347"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ9505588.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4706..5782)
                     /locus_tag="IYE22_000348"
     CDS             complement(4706..5782)
                     /locus_tag="IYE22_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ9505589.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5806..6399)
                     /locus_tag="IYE22_000349"
     CDS             complement(5806..6399)
                     /locus_tag="IYE22_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ9505590.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6403..8079)
                     /gene="mdcD"
                     /locus_tag="IYE22_000350"
     CDS             complement(6403..8079)
                     /gene="mdcD"
                     /locus_tag="IYE22_000350"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ9505591.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8069..8368)
                     /locus_tag="IYE22_000351"
     CDS             complement(8069..8368)
                     /locus_tag="IYE22_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ9505592.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8470..10116)
                     /gene="mdcA"
                     /locus_tag="IYE22_000352"
     CDS             complement(8470..10116)
                     /gene="mdcA"
                     /locus_tag="IYE22_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ9505593.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10123..11034)
                     /locus_tag="IYE22_000353"
     CDS             complement(10123..11034)
                     /locus_tag="IYE22_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ9505594.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11154..12011
                     /locus_tag="IYE22_000354"
     CDS             11154..12011
                     /locus_tag="IYE22_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9505595.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12198..12560)
                     /locus_tag="IYE22_000355"
     CDS             complement(12198..12560)
                     /locus_tag="IYE22_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505596.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12906..14345
                     /locus_tag="IYE22_000356"
     CDS             12906..14345
                     /locus_tag="IYE22_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9505597.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14352..16904)
                     /locus_tag="IYE22_000357"
     CDS             complement(14352..16904)
                     /locus_tag="IYE22_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ9505598.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17018..18349)
                     /locus_tag="IYE22_000358"
     CDS             complement(17018..18349)
                     /locus_tag="IYE22_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9505599.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18315..19793)
                     /locus_tag="IYE22_000359"
     CDS             complement(18315..19793)
                     /locus_tag="IYE22_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9505600.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20098..21933)
                     /gene="tdc"
                     /locus_tag="IYE22_000360"
     CDS             complement(20098..21933)
                     /gene="tdc"
                     /locus_tag="IYE22_000360"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ9505601.1"
                     /translation="MKDMDIKVVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22403..23830
                     /locus_tag="IYE22_000361"
     CDS             22403..23830
                     /locus_tag="IYE22_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ9505602.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLHEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23922..24287)
                     /locus_tag="IYE22_000362"
     CDS             complement(23922..24287)
                     /locus_tag="IYE22_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ9505603.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24284..24649)
                     /locus_tag="IYE22_000363"
     CDS             complement(24284..24649)
                     /locus_tag="IYE22_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ9505604.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24841..25089)
                     /locus_tag="IYE22_000364"
     CDS             complement(24841..25089)
                     /locus_tag="IYE22_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505605.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25088..25216
                     /locus_tag="IYE22_000365"
     CDS             25088..25216
                     /locus_tag="IYE22_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ9505606.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25273..26967
                     /locus_tag="IYE22_000366"
     CDS             25273..26967
                     /locus_tag="IYE22_000366"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ9505607.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26971..27090
                     /locus_tag="IYE22_000367"
                     /pseudo
     CDS             26971..27090
                     /locus_tag="IYE22_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27172..27363
                     /locus_tag="IYE22_000368"
     CDS             27172..27363
                     /locus_tag="IYE22_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505608.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27741..28874
                     /locus_tag="IYE22_000369"
     CDS             27741..28874
                     /locus_tag="IYE22_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ9505609.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28905..29225
                     /locus_tag="IYE22_000370"
     CDS             28905..29225
                     /locus_tag="IYE22_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505610.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29254..29718)
                     /locus_tag="IYE22_000371"
     CDS             complement(29254..29718)
                     /locus_tag="IYE22_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9505611.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29849..31102)
                     /locus_tag="IYE22_000372"
     CDS             complement(29849..31102)
                     /locus_tag="IYE22_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9505612.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31131..31448)
                     /locus_tag="IYE22_000373"
     CDS             complement(31131..31448)
                     /locus_tag="IYE22_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ9505613.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31474..31794)
                     /locus_tag="IYE22_000374"
     CDS             complement(31474..31794)
                     /locus_tag="IYE22_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9505614.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32258..33673)
                     /locus_tag="IYE22_000375"
     CDS             complement(32258..33673)
                     /locus_tag="IYE22_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9505615.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33690..35006)
                     /locus_tag="IYE22_000376"
     CDS             complement(33690..35006)
                     /locus_tag="IYE22_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9505616.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35156..35854)
                     /locus_tag="IYE22_000377"
     CDS             complement(35156..35854)
                     /locus_tag="IYE22_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ9505617.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36143..37132)
                     /locus_tag="IYE22_000378"
     CDS             complement(36143..37132)
                     /locus_tag="IYE22_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ9505618.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37212..38045)
                     /gene="msrA"
                     /locus_tag="IYE22_000379"
     CDS             complement(37212..38045)
                     /gene="msrA"
                     /locus_tag="IYE22_000379"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ9505619.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38220..39854)
                     /locus_tag="IYE22_000380"
     CDS             complement(38220..39854)
                     /locus_tag="IYE22_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505620.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39841..41175)
                     /locus_tag="IYE22_000381"
     CDS             complement(39841..41175)
                     /locus_tag="IYE22_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505621.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12500 a         8475 c         6958 g        13272 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat ctgcgataat aagaacaact aaactaaaat agtgcttcct
     2701 atttttcgtt tggttgttct tattatcgca gatgcaagct tttaaacacc atttagtgga
     2761 gaggttgtga cagagatgtt cagccttgag cgataagaag cgattttgaa aaatcgcttc
     2821 ccatatttta caaaatcgtg ccttattgcc gataggctat ctctggcaca ccgtttattc
     2881 agaaaaagga gctgcgacaa ggcttttgtc acaactccgt tcataaatat ttcctgtcaa
     2941 tccagcataa tcggctgaat aaaatcggct tgccatcttt tttctttctc agaaaaatca
     3001 aaataccata tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat caatcgccca
     3061 tcttttcgat cgactgaata atcacagcat ctgaaatcaa ttcggatggc aaaactattt
     3121 tcgtggatct gtcgataatt tcctacttcc tgaatagtta tttttttagt atgatttctt
     3181 acaccgtctg ctttgttttt ttgcagaact ttctgatgtt cggcaaacag cttgtctgta
     3241 taacagcttc tggctttttc aggagtttct tcttcccaag cggattggat cttcatgaat
     3301 atcttttgga tttccagaaa ctgtgctttc ttttctttag ccgttcctga catgcgggac
     3361 aatatctccg ctttcagcct tttcttttgt cttttattct tgacgataga aaaagcagaa
     3421 acccctccta ctgacaaaac agctaatgca agaaatcccg tttgctcata actagaagga
     3481 cctgtacctg gaccataata cccataagaa tctctgccgt aagaacgact gccaaaataa
     3541 ctgcccccat atgaacgacc accaaatgaa cctctgcccg aacttcgacc gctagatgta
     3601 cctccaccat gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa caaacatagg
     3661 cccactaaca aaatgattgc taaataattg atttttttca ttgtcttcct cgttatcttt
     3721 tattacaaaa aactattttt atgatagcct tgctaaaagg aagagtcaat aaccagatgc
     3781 ataaactgtt tttttagcaa ttttgtctta tttagtcatt gagtaacatc ggacaaatgt
     3841 atgtgtcggc agtccataat tcctctatct tcagcatgaa caagcttgca gacaagagat
     3901 ctgcgcttcc tccaggagag atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg
     3961 gacgatttgg taaagcactt tgactagcag catgagcaag tttttgcatt tccactaacg
     4021 ttttctgatc actgcgatgc aatacgcatg tgtcatctag cgaactcatc aaaactaaca
     4081 acatatgcat ttgtttctgt acatatgaat cattatctag ctccttagct gcttgaattg
     4141 caagcctgat atgcggatac cctaatatcg cttcttcata tgcaccattt acgccatatt
     4201 tttgcttcac cctatgacca tgagtggttt ttttatgacg atattttgta tcaggaaaag
     4261 cagcaagctt gctgacatca tcaagtattt tcgataaaga aaaaggctgt tccatactga
     4321 ttttttgtgc aatgacactg atcaatagcc ccattgccca aatcgctcct ttatgggtat
     4381 tgatgccatt tgtagcaatc agcattgcct tttccgcttt tcgcccaatc tcagcaattc
     4441 tttctcttaa ctcctgatca atagcatgat gccatgctat ttgagccatt tcttcaaaaa
     4501 aaggagttag cgtcttagca gacttaagaa ataaagcata gtccatatcg gaatgtgcac
     4561 ccgcatccag tgcatccact aatccaggtt ttggacttaa tgttacttct gcgatcaatg
     4621 cttcctctac caacgcagcg agttgctgag tcactgcttt ttttcgttcg tcatttatca
     4681 tgacactcac tccttcttcc tactattact tgctgaaacc ttttttgata gctacccaaa
     4741 tgatatgaag taaccagctt aaagcaaatg cacaacttgc cgtcattaga taagctacaa
     4801 gaatcatgac tcctgaaggt aactgcagcc aactatcttt aaacaataat aacagtactt
     4861 ggaaaacaaa tacattggct aaatacgtac gataagcata aacagacaac catttgaaca
     4921 atggcaattt tggcgaatcg tttacaatca ttttggaagc gcctatgaag acagcaaaga
     4981 tgatgactaa actgtataaa ctttggatcg tattcaagta aggtgcattc gcaaagctca
     5041 agtctcctgg atgtgcgagc aatgaacgga cagaaaagaa cgcgataact aaggcaacag
     5101 gtaagaaaag ataacgtact ctgtccaaat attgatagac tttttcgtga taaacaaatg
     5161 cggctacccc ataaatcatg aaactagcaa caaaccgatc taataagtac cagctgtcat
     5221 gataaggccc agtcaatact tgtgtttgat agaacagata ccatgcgaca tataaaatcg
     5281 ttgaaccaat caatactggc catacatatt ttttattagc aaatactttg taaccgagcc
     5341 aaacaaaaaa cggcatgaac aattggatct gcagcatcat aacggtatac cataaatgag
     5401 gcgctccatc acctgtgaca tatttcagta aaaagcttgc agtatccgta tacggagttt
     5461 gttgttggac tgctggaaat agccaaagat aagccgtcgt ccatagaaga tacggaacga
     5521 ctagttcaaa aaacttttct ttcaaaaatt cccaataaga caaatgctga cttgttttta
     5581 ccatattata aacaattgca aaaataaaca ttggtgctgt gtatttaacg atgacgtaaa
     5641 aggtaccaag aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca aagctcaata
     5701 tcgtttgcat cataacagcc gttacagctg atactttctg gaagtcactt acgttgattt
     5761 tgattttttt gttttcaaac atctctttct ctctcttttt ctttattacc aaagttcttt
     5821 ggttaattta ggtccttcga ctgttttcaa tagaattttt ttctctggct ctctcaaata
     5881 ctcttttagt gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt gctcaaaaaa
     5941 agcaggatgc gattgaatta cctgaattgg caattcaatc ggtgaatttg cgtacagcag
     6001 caaatcaaaa tcactggttt cttttactgc cggaatccca gtcgataatt caaatcctaa
     6061 gctgccgccg attccccatt tacatcctga taatatgttt ctagcagcat gaaattgctg
     6121 ataaacagga taatccatga ttttctcttg tctatacgat tcttgttcga tgatgtccca
     6181 cggatgaatg acttgtttga cggcatgttt tgagatttct gttgaccatc tctgattgcg
     6241 ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg cacgacgaac
     6301 tgttccgaac atcttttcag ttagaacgac ccattcaggc aaggtgaacc cttctatttc
     6361 atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat cccattcttc
     6421 gtccattttg gcacggactt tatttgtttc gacacgtccg aatgttttgg ctgtttcgtt
     6481 tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa tggcttcatt
     6541 gattgcttcg atgactttgc tgacattttg ttctgaagca gcttggtcgg tgatatcttc
     6601 taggaaatga tagatcgctc ctagttttgt ataattattg atatcgtaag cgatagctgg
     6661 aactttttca gcagctgctt cgatttcagc aaccgaacgt tttgtcacac gtgcagcact
     6721 ttctttagac atcgcttgga cggtaatttc tttattgttt aaagcaatca agcggcttga
     6781 ttgtaatcca tgtgctaaaa atgcacctga aatagcattt cctgcaatca atccaataac
     6841 tggatgtcca gcttgacgga gttttgcata agcagcagca ctattagcca aagccacatg
     6901 gataccgatc aattcttcgt tatagccgta tgcttggctt ggtacatcga tcacaacgat
     6961 gattggccgt ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca aaatttgtcc
     7021 catgcggaaa ccttcaacta aaccaacttc accgtttcgt acacggtaca tcgggttttt
     7081 aggatcaggt acgattgatg tcacgatggc ttcttctatg aatttacttg atttaccata
     7141 atacacagta ctaatagaag aagttggatt tttcatgcca gttagtgccg caaaccaatc
     7201 gtaaccaaca cttgctgcag ctggttcatt tccttctgct acttctggta agtcaattga
     7261 ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca atggccattg gttttgaaag
     7321 atctaaagaa tcaagtaatg ataaataaaa tgctgagctt tcactgcgat gcgtgtcttt
     7381 tcgttcgtcc attgctgcga tgacagcagc tttgattgct tctacactat cagtcactaa
     7441 ttcatcaatg atttttgatt ctacccgttg acgagatcct aatgtattcc aaatcaagtc
     7501 tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt ctggtccatt
     7561 caaaccgact ctcgcttttt ttgttgcgat taaataactc attaatgctg aagtaatcga
     7621 cataccgcca aatgatccta cacgtcctgg aatcaaaccg atgactggta catattgttt
     7681 tagagcaatg attacatttc cgatttctga aatagataac agtccgtagt ttgcttcttg
     7741 caaacgaaca ccacctgtat ctagtacaat gactggaaag atctcatttc cttttttatt
     7801 ttcttctaac acatgttcaa gtgcagcaac gatttttgca ccggaaactt caccgatacc
     7861 gccaccttgg aattttcctt ccatagcgat tactactgct tgtttgccat tgatcgtgcc
     7921 acggctgaca acgatcccgt catcactttc agggacgatt ccttgtgctg gtaaacttgg
     7981 cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta gagcgttcac
     8041 tcgttctctg gcgtttaatt ctacaaaact atttttcatc tgaaagttcc tccattgctt
     8101 gcgtcaatcg taggtacaca actcctggtg tagcaccgaa gtcattgatc acgatatctg
     8161 ctgaaattgg ataacgatca aaaaatctcg ttaatacatt gttccatact tcgccgaagc
     8221 catcgcttcc tgtacaaatt tttacagtag attctgtttt ttctgttgga aacatcaaga
     8281 tttccaaatc tcctgaaccg accactccta catggatttt cttattgatc ggctgtttcg
     8341 ttggatattt ataagctaat tgttccatgg atctctcccc tttttgtaaa acagagcata
     8401 cacgttctgc ttattcgtct ttcttcttcc atctgggtaa atggcgacaa aaggtttttc
     8461 tcgccaattt tatgaccaag ttctgaattt cattggtggt tcatacaggc catctgacca
     8521 ttcgatcaaa tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt cactacgttt
     8581 gatgcctaga tcttctggta atttcacgat gccggctgca cgcaagctat ctagtgattc
     8641 tttcgtgcta gtcattccaa gaggtgtaac accgccaatt gctgcaattg ctgcttgtcg
     8701 ttcttctttt gtttttgctt tgtacaaata agcaattcct tcttctgtca cgatatgtgt
     8761 agtatcttcg ctataaatca tcactggtgc attggctaag cctgcttgtt tttgaacagc
     8821 tagtgcatct aattcttcga caaagactgg ttttttattt gaaccatatg tttcaaccat
     8881 ttgaacaact aatttttggc ctttccctaa tggatcatca ttgtcgcgaa gtgattgcca
     8941 tgctggtgta gaatgacgac gaccgccagg attatgtccc atgttaggcg caccgccaaa
     9001 accagataaa cgtccttttg ttacggtaga agagtttcct tcgtaatcta attgcaaggt
     9061 tgaaccgata aacatatcga tggcatattg tcctgcagct tgtgataatg ttcggttact
     9121 gcgcatcgtg ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa tatatttttc
     9181 cataccaact tcgccaccaa aactatggat gctttcaacc caacctgatt cgatggctgg
     9241 aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg attttccctt ttaaccctag
     9301 tgattcacca taagttggca gtaacaattc gatagctgct gtattgaatc caataccgtg
     9361 attcaaagat tgtacgccgt gttctgcgta gattcctttg attgccatca ttgccattag
     9421 aatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta attgataagg
     9481 tttatctgct ttcacgacaa cgtctaccca gtcaccaggg atatctacac gtggtaaaga
     9541 atcgacaact tcgttaactt gaacgatcac gataccatct ttgaaagctg cagcttcgat
     9601 gattgttggt gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt tatctgcttt
     9661 atctgcagct actaaaacaa catttggaat caaatcgatg aataagcgtc cataaagttc
     9721 caaataagta tggatatcac ctaatttcat tgtgccgtct tcaatcattt gggcgacacg
     9781 caagctttgt gcaccagcat aagagaaatc gatcttgctt gcgattcctt tttcaaaaat
     9841 attcaaatgt tctggtcgtg agatactaga catgatcaaa tgcagatcat tcactttttc
     9901 tgcatctact ttttcaagac tttctgaaag aaaacttgct tgtttttgat tgtcgccttc
     9961 taatacaact ttgtcgccgg gagcaatcag tttttccatt acttctacga ttttttcagt
    10021 ttctacatac ttgccattga gcaagttgct gattttttcc aatcgctgcg cttttttttc
    10081 gcgtttagaa gcccagttgc ggtttggttt ttccataatt agtcatctcc atttttgata
    10141 tagaaacgtg gcgtcatcaa taccatgttg gtctagattg atcgtacgga tccctttatc
    10201 tacaccaaat tttgtatgaa tcagctttgt caaagtattg ccggggggaa attcaatcgt
    10261 cagatccatt cctaattctt ttgccaagtc catcatttgg ttccactgca cagggaaagc
    10321 taggttttcc aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc ttccatccgt
    10381 attttttata tacaaacatc tagcatcaga tagatggaca gactgcatat acggcttgaa
    10441 tttcttgacc gtatcattca tcaagagaca gtgagatggc acagggactt tcagcagctt
    10501 cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta acggataaac cttcgatact
    10561 cccagataac gtatgttgca tcggacaatt ttcatttgac agataaactg gatggtcagg
    10621 atcaaatgtt tttgctacaa tttcctcagc ttcgcttctc gtcacaccta ctactacgcc
    10681 cattgcataa ccgcttggat aagcttcttt cattaagact gcgcggtgat agactagttc
    10741 cgctgcattc tcaaatctca aagatcgaca agcaacagct gcacaaaaag caccaatcga
    10801 atgtcctgct gcaatatcag gctgaatacc gtttttttcc atttcatcta aataaaatac
    10861 tgcttttgtc agtaatgcta gctggataaa aaccgtatct tgatagtttt cttctttatc
    10921 aactaaccga taccccgtca cattttctaa acgttccatg tacatttcag ggatatccgc
    10981 taacatccct aacttctgac tgccttgtcc aggaaataag aaacctgtac tcatgagcct
    11041 cgcctcctca gcacttactt tttccactgc tatcttagtc aatttctgct ataataaaaa
    11101 atgccaaaaa aagaaaaaac cataacaaaa aggtatggat gggatggata attatgaaat
    11161 tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc caagcagcca
    11221 aagaattatt tatttctcaa cctgccttga gccgtgcgat caaacatttg gaaaacgaat
    11281 taggtgtccc tttgttttac catagcggca gaaacattga gacgaccgtt tatgcagaag
    11341 aattttatcc atatgcagtc aagacattag acacattaga agaaggaatc aaattattgt
    11401 cttcggtcaa tgagaagatc gttacttccg tcatattgta tttagaggta gcctctgttt
    11461 ctattccgaa cttggtccga atttttatgg aaaagcatcc tgatatccaa ttaacgatca
    11521 tgcaacatca tttaccagat gatattaccg atcctgtact gtacatcact tcggagatga
    11581 agcctggcat gaccaatatt cctatcatga aagaacctgt ttatgtcgcc attcctaaga
    11641 aacatcctct tgctaaaaaa gaacatctgc aattagaaga tatccaacgc acccccatct
    11701 tgatgctgtc aaagagcaac gccttccgtc atacactgga tgcagcactt gaagcgaaaa
    11761 gtatcgaatt gacgatcagt tcgacgacag atgatcctgc gacattacgt tctattgtca
    11821 agcaaggatt agctgttagc tttttcccaa aagtttcttg gtcttacgat aaaaatgatc
    11881 ctttcgtctt gcgggaaatc aaagatttgc ccttgacacg aacaatttat ttatcttcta
    11941 gcttcacaga agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg gaattttatt
    12001 taggaaaata aaaaaaaaag agctgggaca aaattcttgt cgcagctctt ttttcgaaca
    12061 aacggtgttc cagaagtaat ttcttagaaa ataagccgaa atttcccaaa aatttgagaa
    12121 gcaattttcg taaattcctt cttatttctt gaagctaaac atttctgtca caacctcttc
    12181 tctattgtcc tagacattca ttgcgttgat ttaaaacttc catcaatcaa atctgctagt
    12241 tcatctagct tttctatttt acgtagatca atagagatcc aatgttcttt attcatgtga
    12301 taggctggaa aatacccctc ttttcctcgc aaaattccgc caagttcagg atcgaccttc
    12361 aaatcaagaa tattgatgat cctatggtca cttgcatcat catagatttt ttcttctgga
    12421 acagacatca ggaccccaaa ccattttttg cttttttgat gtctgaatac gcaataatca
    12481 ggaaattttt taaaaatata ttctggagac aaattgtatg tttcattgat gtgatcgatt
    12541 agttcttttt tcgttagcat ctgcttccat ctctttctta ttgattcgct ttattcaaag
    12601 ataccacgta agaatagaaa aatgaataca agtagttgct aatcaaatac ttcgatattt
    12661 ttctttttta tcaactctta aatgttcaat ccgcctgtgt tggtcttgct accatctact
    12721 attaaaatgt ttattattgt ttgcccacct attttttgat tttagtggta tcatttttca
    12781 agttcatgat gaaattctta tcatcagtaa atgctccaga aaaataaaca atgattgaaa
    12841 aatcagttga aacttgacca ccgtttattt tctccgtatt cttagaatag aagggagtct
    12901 gattgatgta ttcactaatg aaaaaaatca ttaccgaaaa agatattcga cgtcatgttt
    12961 ctttagtaga acaattgctg aatcatacaa agataaccgt aaatgaatta gctgaaatca
    13021 tagggacaac cgaacgaacg attttttctg accttcaatc tattcgctct cagctgcccg
    13081 aaggctggga catcatcagc gatcaagcag gaatcagttt gcaaaaccag caaaatttat
    13141 taacaaatga cctttgggag atttttttca aacaatcagt cagtgtcgaa ctgctgaaaa
    13201 atctgctttt tacaaaaaaa gttgctgtcc ctgattttct agccgaccat gggctttctt
    13261 atgggacatt gaaaagacat gtgactaaaa tcaatcagcg tttagcaagt tacgacttgc
    13321 agatcgattt gacgaaatat acagcttgta ttcttggaaa agagcgtgcg attcgtactt
    13381 tttatcatcg attgctgatt cctttcactc ataacaacta ttttttcgag gattattcga
    13441 tccatgaatc ccattatttt caatttttaa gaaacctcag tcaaactgaa ctagcggttg
    13501 aaacagaaga gattttcgga acatgttggt tctttatcaa tacgatccgt ataaaagcga
    13561 attgccgatt agattcttct atccatacca acagcacact ctcctccctg tatgactctg
    13621 cattgaaaaa attgtattta acggaaggta tttatcttaa agacactgag ctttcttttg
    13681 cttctttctg ctttctggaa agttggaatt acaacaataa ctatggacaa gaaaccgctc
    13741 gctgcctgca tcattctcca tttttagagg tcctcgaaac gtttgtagag gaactagctt
    13801 cagagctgtc tcttgatcaa ctaaaaaaaa catctctgac tgataacttg gctctcctca
    13861 tattaaaata tcatgaatcg ccgatattga ttgaagcatt ggaccgtcaa taccatcgtt
    13921 ttttagaaac atatgatcag caatatgcac acttgtatca acagaaagag aatttactga
    13981 acgatctaaa aaaagaaata gaaatcaatg aaccaaatta ctttctccaa ctcttgtctc
    14041 tactgatcca acaaacgatt ttttctatca aaccgaattt gttcaatgtc tattttattt
    14101 tccaaggaga accttcatgg aaggcgttct tgcagcagga attaaaagat tacttaggaa
    14161 aacgtgtctg cttcttacct atcgaactaa ccgatctttc cactatttct tttgaaaaaa
    14221 cagatatctt gatttccaat ttccctctag attctctaga tatccctatt gtatacattt
    14281 cttctattcc aacgaaaaat gagctcaatc gtttgactga actcactttc aaaacttttt
    14341 tatagtaacg gctagcgact gtattctcta gctgtttttt cttttttgcg aaacagtttg
    14401 ttcatgatcc ctttttctat ttctacaact aagaatagaa gaatcacatt tgctccaatc
    14461 aataactgtt gtgtcatcgt taaacttact gtaccgatca gctgctggcc gactggtaga
    14521 aaaataacca tcccttgcaa taaagcaagg atacctaatg aaagaaaaag cgctttgttt
    14581 tgcaaaagac cttggttcaa tgacggcttg gacaactctc tgcaattgat catgtaaaca
    14641 gcttgagcta agacgatatt ttgcagaaga atggtttgct gtaagctttg cccttcaaac
    14701 tgcatagcca ataaataggc aggaaccatg atcagtaggg agacatagat gatccgaaac
    14761 aaatgataac cagataggat tccttgcttt ggatctcttg gtgggcgctt catgatttct
    14821 ggatctggcg actcgaaccc tagtgcataa gataaagtga ttgtcgttac catattgacc
    14881 cataaaatct gtactgggga caaaggtagc gggcgattca gcataagtgc ccaaatcaag
    14941 atcaatcctt gagctaatgc agttggcaag aagaaattga tggttttttg caaattatcc
    15001 aaaatccgcc ggccctcttt tacggctttt gcaatcgtat ggaaattatc atctcctaaa
    15061 accatatcag ctgcctgttt gcttacttca ctacctttga ttcccatagc aattcctaca
    15121 tcggatcttt tcaaagcagg tgcatcattt acgccgtctc cggtcattcc gacgatttct
    15181 ccatttttct gcaaagcttt aacaattcgc agtttatgtt ctggtgtcgt tcgagcaaag
    15241 acatcgacta ttggcacttg ctgaatcaac tcttcatcag acataagatc caaatcgatc
    15301 ccttctaaaa ctttggcagt atgttttaag ccaatttgtt ccgcaatcgc ttttgctgtg
    15361 tctttgtgat cccctgtaat cattttcact gagatccctg cttgttggca ttcctttaca
    15421 gctttgactg cactttcttt tggcggatcg atcaatccgg cgatccctgc aaaagttaat
    15481 tgggttaaag tctcatgcgt gagttcctgt ttcgaatcta cttttttata agcaaatcct
    15541 aaaacacgct gaccttttct cgcaaattct gcagctgtct gactccatgc ttcttgttct
    15601 gcagaagaaa gagttgattt agcaaataat acttctggtg cacctttgac gtaaatcacc
    15661 gctccttttt cctccgcatg catcgttgcc atatatttgt attcagaact gaacggaatc
    15721 tttgcttcta tttttttcaa aggtagattg gctgtttctg tgtgtcgaat cagcgcaact
    15781 tctgtcggat tgccacttaa attttctgtt ttctgttctt cttttgtttt taattcctga
    15841 cagtttttca taatggacaa cacgcaggct tcttcttttg cagctacatc cacaactgtc
    15901 atttcatttt ttgtcaatgt tcctgtctta tctgaacaga tcactgtcat agaacctaat
    15961 gtttctaccg aaggaagtcc tttgatgatc gccttttctt ttgccatttc atgtactcca
    16021 acggatagaa tcatggtcaa aacagctggc aatccttcgg gtaccattga caccaccaaa
    16081 gcaatcgtag ccgaaagaag gatatgccaa tccataccat atcgaaatgt cgtaaaaata
    16141 actaagaata ggctcaaaca aagaatgcct tgaaaaattt gtttgtttaa tcggttgatt
    16201 tttttaatca gaggcgtcgt ttgttgttcg accgattgca atgcttgatt gattttaccg
    16261 atttctgtcg tgtttcctgt ttctacaacg acaccgactg cagaaccaga ttgtacaagc
    16321 gttcctgaaa aacccatatt tttctggtcg ccgatcgatt cttcttctgt caaaacggca
    16381 tgactttttt cagatgcttc tgattctccc gtcaaaatcg attcttccac taataaatca
    16441 tgtgcttcga ttaatcggat atctgctgga acgacatctc cggcacgtaa gacaatgaca
    16501 tctcctttta ccagttcttt cgcagggatt ttttcttgca tcccgtttga tagtactatc
    16561 gcttcttgtc ccatcagctg cttcaaacca ttcaatgatt cttccgcttt tctttcttgc
    16621 cagtaactaa cgagtccatt gacgatcact acgaataaaa taatacttcc ttcaatatat
    16681 tcactagaag caaatttcaa aaaagaggca atgatcaaca cgatcatcag cagatcagta
    16741 aaatgctttg ctattttttt ccatcgtttt gtctgctgcc ttgcttttat ttcattatat
    16801 ccttgatcag tcaggcgttt ctgtcgttct tcttgcgaca agccttctgt tgatgtttga
    16861 tagtttttca atacatcttc taccgtcaat ctgtaccagt tcatcctatc ccctcctgtt
    16921 taattgatac tgttattata cgcagggaag ttttttcagg cgtatcgatt atttcagggg
    16981 ggtctgaaat aattcgtttt gcaaaatgta tctgggccta actgatattt ctattgctta
    17041 ttcttttata tacaaaattt aagaatacaa accagataac caataaaaaa ggaataaagc
    17101 gtgtcgtgct ttcgaataac aagctgaaag caattgaagt aaaaaacacc aacaaagcaa
    17161 gatctttata tctttgtgta gtgttccgtt tcttttcttt aataaattga acatgtgcac
    17221 acaagatcga agaccacaca aatagaaaac aactcgttgt catgcctgcc aaaagctgaa
    17281 aaaacaatcg actatcagct acaaaaaaat gtagacaaag accggcaaaa aataacattc
    17341 ctgtaattaa aataccgtgt aaaggtacgc ctttcttaga aagcgcacaa aaccaatggg
    17401 gcaaactttt ttcttttgct gtttgatata aaatcctgct agtactgtag actgcactat
    17461 ttgctgagga caaagaagca accaaaatca ttagattgac aattgctgct ccgccttggc
    17521 aatggatcac ttgcagcata gtcactaaag gactttcttt taaatctaaa caatgccatg
    17581 gaacaacaaa taaaatcgac aaaatcgttc ctatatagaa aaagagaata cgccaaggca
    17641 gacagttgat tgcttttttc aaattctttt gcggatcact tgtctcgcca acggttaagc
    17701 cgatcatttc aattccagca aaactgaata aaaccatagg aaatgagact accattccag
    17761 caaagccaaa cggtagtaat tcatcgatct gtccaaaatt ttctttgatt ccgatagact
    17821 gggtatgaaa atctgagaca ctcaatccta tacccaataa gataaaaagg aaaatcccca
    17881 agatcttgat gattgagaaa aaagattcca attcaccaaa ccacttgact gcaacaaggt
    17941 tgactgccat taagatcatt agtgcgctac ctccgcttat caaagctggt acatgtacaa
    18001 accagaaatg gatatacaat cctattgctg ttaattccgc aagtccagct ccaagccagc
    18061 aaagccaata agtccaattg acaaaaaatg cccacttttt gcctaaataa tagtcaacga
    18121 aatcactgat agagttaaat gtactatttg ctaaaaataa atcacccatg gcccgcatca
    18181 aaccataaat agccaaaccg catatcaaat aaacgagaat aacagctggc cctgctgatg
    18241 caattgcctt tgctgaacct aaaaataatc ctgttccaat tgcgccacct aatgcgatta
    18301 actggatatt tcgattagtt agagtccgtt tcattgccgg tttttccatc aagttcacct
    18361 ttttcttttt caagcagcat ggcgacttcc tgttcccaag aaggtttctt tattttttca
    18421 atacagagtg gtactacaaa caaaatgaca ctgatggctg tgactgcaat tagattgatc
    18481 catgttccag ctttcatcgt cagtccgaca gaaaatccga ttgccgcaaa taaaacaata
    18541 acgacagtcc aaactccaag attgctcttt ttctcttttt ttccaattct aaatggtcga
    18601 ttcaactttg catgtgtata acgcaatctc aaaactgcga tccccatgat gacatagacg
    18661 atacagtaga taacagtagt tgaatttacg agcatcaaaa atgcttgatt gacgccagga
    18721 atcagcaaat aaaaaagtgc aaagattgag ataatgacag actgagtcaa aataacggat
    18781 ttggatacgt caaattgatt cgtcttccaa aaattgaatt ttggtggata cgcacctttt
    18841 ctagcacttt ctgtcactgt ctttgctggt cctgaggccc aagcagataa ttggactgct
    18901 acaccaatga agacaagtaa actaaacaga ttcacaataa gatgtggcaa accaagaatt
    18961 tggcaataaa tcgaaataga ttgcgcgata ttattcaatt ccatttgtcc ttttggtacg
    19021 acatttgcta caactaaagc attcaaaata ttgacgaaaa atataaaaat taacgcggca
    19081 aatactccgc gaatatacgt tttaactgga ttttccaatc gagtgatata cacagaggac
    19141 atttcgatac cagtaaaaat aaacatgatc ggcgcaaaat aaacaaatga ggatgcagtc
    19201 gtagtatctg gtattaattt gtcccatgaa aaactgccta atgttccggt tggtacgatc
    19261 ccaactttga tccacgcagc aaaccctaat aagagcatca tcgtcaaagg cacgtacaat
    19321 cctaaccaaa caccgacttt tccgccgatt tttgccatat caaatttcaa attcaaaaaa
    19381 gtgatgatcc aataaacgac taaaatgaca actaatgtaa atttgctgtt caaaccaagc
    19441 ggcacattat cgataatatt accaaacaac ggagcgagtg ctgaagcgac catcaccatc
    19501 ccaggaaaca tttggaccca taacagccat gagacaacga acccccattt ttttcctaga
    19561 gcattcgata cccaaagttc tggaccacct ttttggggaa acattccagc aaattcacca
    19621 gagatcaatg agattggaaa agcgtacaat aatactgcaa ccagcatgta aaaaatcatc
    19681 gtccagctcg ttgctgctac atctggaata tttcgtacac ttgctacaag ggcgcaggtc
    19741 attccgataa aggtcatggt cgtgatttgc tttgatgaac cagtatcttt catctttatt
    19801 ccttctttca atagcgacat atgtttttgt tttcttttat ttttgttcaa gaacagaaat
    19861 gacaaggttt catcgattca gtacaaaacc cagtatcacc gaaacatcca ttgaacgatc
    19921 ttagtgaact gggtttgata catgaaacga acgatccaac ttatagaacg ttttataaaa
    19981 aagaatatct tggcgaggaa tatatgaaaa acagggggaa atacaattgt tttgatgaag
    20041 caaataattg tctgttctcc atatgtctct tgttattctt tcacacaaaa atcagttcta
    20101 ttggatgatt tcgtttagtt tcttttccat tgctttggcg attttttttg tataaaaatt
    20161 gaaaattcga tcatcattca aataaggagt catgatagcc gcacgtagca gagtgatttt
    20221 ctgttctttt ttccattctt ctttcgttag acccattctt tcaacaaaac gaatcggaga
    20281 atcaccgtaa tcttcttgtg taaatgtagt atgagaagta ataaaccgtt caccataaac
    20341 atcgccatcc atataagaag accgatcgaa cattttttca tttaattcat tgatagcgtt
    20401 caaatctgta cagccttgtt ctttgaatac ccaattgacc atattaaaat caggatgatt
    20461 caatggatag acttcgattg tttttccttt aacagtaaaa gtcagatgat cgagaaattc
    20521 tctgaatctt tgagctgcct cgattgaagc cccaatcaat tgaccatatc cggtcacatt
    20581 caatggcaat acacgatgtg ctgtccagac tgcggcggcg gttgctccag cttttgatcc
    20641 ttccaaaata taagctccta acatatctgg agcctttact gatttttcaa acacatatgg
    20701 cgcgaaataa gaaatgatat ttctcatatt tttatgtttg attacgattc ctccagcagc
    20761 ataagggaca tagcccatct tatgaggatc tatcgtgaca gaatccgctt ctgaaatagc
    20821 tctgaaccct tcatatacct ctttgtcaat cgttacgcag tggtggaaaa catgatgttc
    20881 ttcaaagaat tcagctaagg aagcatacgg cacaaattct cctgcttcgt ttagaaacag
    20941 tgaacgcgca tagccgccat atgcggcatc aacatgtaaa tagaaataga tcccttcatc
    21001 ttttaacctt tctcttaatt ggacgatttt atctacactg tctacttgac cttcttctgt
    21061 cgttcctacg acagcaacca cacctagaat tgggattttt tgtccagcta attcacgaat
    21121 tgttttttcc aaagcattaa tatccattcg ataatcctct tgaactggga tagcaaccat
    21181 ctgatctaag ccgactccgc aaatatctaa cgccttcatc caagaatagt gttttgtttg
    21241 agggaccagc catttcccta atctttggat attttttccg ctacgggaag aagctgcttt
    21301 gacctcatcc atttcttcgt ccgtaaagct ttcggtcatt tccaaaattt cttcaacaga
    21361 taaattcaaa agttcccact cagacatttt tttcactttt tcaggcagca cttccttgac
    21421 agctaaagga atcgatttga tgcaacgggc gtaccataat ccttcaagat tagcaataga
    21481 accatctgct gcaatgtgtc cccatccgtc tgtcatattg aataatgaag caaaatcctg
    21541 tcctacttct gcttccattt gcgaagttgc catagaagat tccaatgcaa cattatttgg
    21601 gttccacagc atcgcatagt tgtaagctaa taaagcaggc atcaatgttt cagcattcat
    21661 ttgtccccag taacggcctg ctgaatgcca tgggatcgat cctgcgcgta atcgctgact
    21721 gacttcatcc aagacctcta tcatatggtc gcgggtggct agataatctg gagtgagctt
    21781 atctccttcg ctaattgcag gcatatctga aggaatataa ttttctctcc atccaagatg
    21841 ttcatctacc atcttgttca acaacatttt ataaactggt ccattttcag ctttgtcgcc
    21901 tataaagacg accttgatat ccatatcttt catttttctt ccctccatat tttgttggtc
    21961 aatgtcttgc agatacatgt gtttatctgt aacacctctt cactatagcg ccaaaaatga
    22021 gaattttttt tttataacat caaatccacg tagattttta caaacaaaaa taaataaagt
    22081 cggatttttc tgataattca acgcaaaaaa gcgaacaatt tttcaataaa tcagtcttct
    22141 aaaatagata acctaaaaaa agcgaacaaa caaataaaat gactgtcctt cattcactaa
    22201 acatattttt atttttttat attgttaaaa attcgcaatt tattttttaa tagaattatc
    22261 tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat gctttttcag
    22321 aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa
    22381 aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata ttttaagaca
    22441 gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa
    22501 tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa aaaaagaatt
    22561 tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga
    22621 acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac
    22681 ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt
    22741 catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt ttaatgactg
    22801 cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct
    22861 tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt
    22921 tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa
    22981 agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc agcaagtgtg
    23041 tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta
    23101 tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc
    23161 ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc
    23221 ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact
    23281 agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac cgttcgaacg
    23341 tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa
    23401 attttatctt ttacatgagg aacagacaaa tttatataaa gaagtccaga caattttttc
    23461 cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt
    23521 tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt
    23581 cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt
    23641 cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca
    23701 gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata
    23761 tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca
    23821 aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc
    23881 atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt ttggacatct
    23941 acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc
    24001 gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa
    24061 acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc
    24121 tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg
    24181 attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca
    24241 agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc
    24301 ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa
    24361 tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga
    24421 tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc
    24481 ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa
    24541 aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa caaatattta
    24601 aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata tacgctacct
    24661 actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt gttcgtctat
    24721 ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact ctcccatttt
    24781 tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc
    24841 ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg tacccaaatt
    24901 tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc tatttttatt
    24961 tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt ctcgtcctat
    25021 catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa acaaggaggc
    25081 gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg
    25141 cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt tatatgatga
    25201 attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt ggaagacatt
    25261 ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat tgccaatatg
    25321 gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat caccttttat
    25381 tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt ggatgaatat
    25441 tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg
    25501 cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc ttctgttact
    25561 gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca ccgaaccttt
    25621 gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa actgggattc
    25681 aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc agaagaagaa
    25741 aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt ttttgaaaca
    25801 aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc tgctcaagaa
    25861 ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca tctagtgggc
    25921 gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat cgagtacttg
    25981 aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga attcaaagaa
    26041 acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac actgactcaa
    26101 gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa tgggtccatt
    26161 acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat ggattatggt
    26221 gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg aaatcctgac
    26281 aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac
    26341 catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa tgccttgaat
    26401 tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa tatgtctatc
    26461 gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt tatttggggt
    26521 tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat taaaatgaat
    26581 ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc tggaccagtg
    26641 aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat ccctgtctat
    26701 atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt
    26761 cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc ttatcagatg
    26821 atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt
    26881 attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc tactctagct
    26941 cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc cctgcatcaa
    27001 gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt ggacttatta
    27061 ccgcagatgc aagcttttga acaccgttta ccaagaggtt gtgaaaaagc tgccctgcat
    27121 caagtaataa gaacacccaa gctaaaactg cttcttatgt ttttgacttg gttggactta
    27181 ttaccgcaga tgcaagcttt tgaacaccat ttaccaagag gttatgaaaa agctgccctg
    27241 catcaagtaa taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttagac
    27301 ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt
    27361 tagtttcgag tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt
    27421 gacttaatac cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc
    27481 tttttgaaaa caactatttt ttcaacgttt actctcttgc caatccaaca tcatctaacg
    27541 gatacaacta tttcaatgat taccattaca ctaaaaaatc ggaaacacac ctctccgaac
    27601 acaaatacat aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa
    27661 ggtaagatct cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga
    27721 atctgaaagg agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa
    27781 tcagaaacgt agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag
    27841 caacagccat ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa
    27901 aagggtatgt tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc
    27961 aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc
    28021 atttctgcct gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg
    28081 gcgggctttt cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc
    28141 taagagttcc ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg
    28201 aaaaagtttt atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag
    28261 gagtaaaaaa aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc
    28321 aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc
    28381 gtctagataa agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac
    28441 aagctggaac agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg
    28501 taggaatgga cggattagag cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa
    28561 gcggcacaat ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc
    28621 aagtaactgg tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc
    28681 gggatatcgc tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg
    28741 caatgattgc agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat
    28801 cagaagcagc acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg
    28861 ttttgaaacc ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac
    28921 gaaataccca tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct
    28981 tccgcttcta tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg
    29041 ggattttcta tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca
    29101 cctattttta tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa
    29161 ggaacatttt tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg
    29221 catgagaatt tttcaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag
    29281 acgagcggtc gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc
    29341 ataaaacgtg gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc
    29401 ttttctgatt tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac
    29461 aaacagggca caaagatttg gtgtcagatc agggcggtta tggaagtcgt tttcaatgac
    29521 gcccgctcct ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact
    29581 ttccttatta gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca
    29641 ttttgatgaa aaccaattcg cgctttgttt tactaattct gggtgttctc ttaatttgat
    29701 ccagttatat gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat
    29761 acaagtcttt atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa aacggaaaac
    29821 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
    29881 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
    29941 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
    30001 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
    30061 caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca
    30121 cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca
    30181 agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta
    30241 aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca
    30301 cgataaacgg tgcgctatgc ccagctgcga ttgcattaac gtttgcttca atggctggcg
    30361 cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata
    30421 agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa
    30481 atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa
    30541 aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa
    30601 atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc
    30661 cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac
    30721 cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac
    30781 ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc aagaaagcaa
    30841 tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat cctttccatg
    30901 attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt aagttattga
    30961 tcattactgc taatgctcct aagatcatta acggcatact caccataaat gagtcccgaa
    31021 tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa tgtttctcca
    31081 taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga ttacgcgttc
    31141 aatcgacgat acagatcaat gatctctttt gctaaatctg taaaagctat agatgtcatc
    31201 agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct
    31261 tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc tgcatctttg
    31321 attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc ttccattgca
    31381 tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag atttttttga
    31441 tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt tttctgcttg
    31501 gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta catctaaagg
    31561 aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc
    31621 taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg aagcagatac
    31681 agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt ttgtgactag
    31741 gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt ccattttaaa
    31801 cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac tttaaaagcg
    31861 ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac acactaaaaa
    31921 agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt taacgtgcac
    31981 aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt attcaaaagc
    32041 ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt tttactttgc
    32101 tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac ggtgttcaat
    32161 cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta tttcaaataa
    32221 ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt tcgtcaggca
    32281 aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt ttcaatgttc
    32341 tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta aatgcattca
    32401 ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt gcgccttctt
    32461 ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg taatcatctt
    32521 gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata ccgttttctg
    32581 ttatcagcca agggatatta tgataatttt cttttagata catggctata tcatacatga
    32641 ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt aaatcaaaga
    32701 cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa cgaggggcgc
    32761 gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga tgctgaatcg
    32821 tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac aactccagga
    32881 actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta taaaacaaat
    32941 catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag accatttcag
    33001 gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc tctactgctt
    33061 tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg ttttgttcat
    33121 gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt tcgttgaatg
    33181 taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca tacttagcat
    33241 aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga aaagctggga
    33301 cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa gagtcgatca
    33361 tttcacttat atgctgagca tattcttcat ctactataca gttctcataa tcagtaaaaa
    33421 aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc atcagatcca
    33481 catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac ccattatgcc
    33541 aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct tctttttttc
    33601 ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa ttttttggaa
    33661 tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac acgatttgct
    33721 acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag ttgcgtaata
    33781 actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg cattggccaa
    33841 gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata accaatcgtt
    33901 ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag catgatcggc
    33961 ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga taacttggtg
    34021 atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa accgatcgtg
    34081 actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat aatgttcggt
    34141 acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa aagcaacggt
    34201 tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa gacttgcgca
    34261 ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa tgggcgttgt
    34321 acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat gataaatccc
    34381 gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa actagcagaa
    34441 acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt cctcaaccag
    34501 caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc tttcgtatct
    34561 gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt cactaattca
    34621 taaacagttg gattaacaga taaataagac atcactgcga tgataccagg gaatacacca
    34681 tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata gatggtcaag
    34741 aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc taaaaaacgc
    34801 aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga aaacaatgtg
    34861 ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt tatcaccatg
    34921 atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc taatttatcc
    34981 ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca tctctttttg
    35041 tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct tttttaccaa
    35101 atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg aactgtcaat
    35161 tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc atatggacag
    35221 tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg atctctgtat
    35281 gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta aacaaagcaa
    35341 ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta gcttctgcaa
    35401 tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct cgaaaagaag
    35461 tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt gcatattccg
    35521 ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag tctcgtatca
    35581 tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc cctcgcttat
    35641 cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa gctagagccc
    35701 gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat tcatattcat
    35761 tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg atcttgtcag
    35821 tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt tcatctgatg
    35881 tattttttcg taattattat aacacgtttg aatcatctga tgattttttg tatacaaaat
    35941 tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa aagactagga
    36001 taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaaaat atgatgttca
    36061 ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta atatatggat
    36121 aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat tcttcttctg
    36181 gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt aaccgacttg
    36241 ctccgaccag tacacttgta acagattttt gtcgcaaatt ccatgcaagg gccatttgcg
    36301 ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt tccagagttg
    36361 atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta gaatcttcag
    36421 gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg aacgtgattg
    36481 cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt gcacgagaaa
    36541 acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt aaaatttcag
    36601 tcattttttt tgtatcttct ccattatagt tggatatacc aatatataat gcttttcctt
    36661 gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa aggtgtatca gggtctgggc
    36721 gatgatgata gaagatgtca acataatcta gccccattct ttggagactt tggtcacaac
    36781 ttgaaatgat ggatttacga gagccccatt ccccatatgg cccttcccac atatgataac
    36841 ctgctttgct agaaataatc agttcatcac ggtaggcttg aaaatcttct ctaaaaatac
    36901 gcccaaagtt tttctctgca gctccagcag ggcctccata attattcgca aggtcgaaat
    36961 gggtaattcc catgtcgaat gcgcctctta aaatctctct ttgattatgg ataggatcat
    37021 aatcaccaaa attttgccat aatccgagag ataaaatcgg taatttcaaa cctgaatgac
    37081 cggatttacg atattccatt ttttcataac gtgcactatc tgcaatatac aatttttctc
    37141 ctcctcatca aatcttttta aaacgctttc ttttttctat ttttagttta ctacatttta
    37201 aagaaaagga gttaatcaaa atgatttttt cgtatatttt tttgccattt gttttttact
    37261 cgttgataga tcagaaattg attacgtgct tgctggattt ttttataacg tttcggttgt
    37321 tttttgtaaa attgctgatg atagttttct gctggccaaa acgtaccggc tggttcgatt
    37381 tttgtgacga ttggttggtg gaaagttcct gaactgtcta gtttctgttt tgattgttcc
    37441 gctaattctt tttgtcgttc gccttgataa aaaatcactg gacgatattg cttccctcta
    37501 tcttgaaatt gtccaaatgt atctgtggga tcagtaattt gccaatagat tgtcagcaac
    37561 tcgctataac ttatttctct tgtgtcataa atgatctcca cagcttccac atgacctgtg
    37621 taaccaccac ttacttgatc ataatttggt ttttcaacat gtccgcccgt gtatcctgac
    37681 aaaacagata ggatcccttt tttcgattca aacggctcga ccatacacca gaaacatccc
    37741 cctgcaaata ctgcacgttc ttgatagtca gcatcttgca tggcatgctt ggaaaagtca
    37801 tatttttgct ccttatcaaa tccttccgta atcaataaat aaaagtccat tacatcaggc
    37861 gtcagattca ttcggatagc taatggacgt agtgttgctt ctagttttga taactgttct
    37921 ttaacggata tattttctct tatgtcattc tttgtctgaa tcaatacttt tcgttcccaa
    37981 tctcgtgtat tcggattcaa aatcagatta taaagtgttt gaatgaatat ttgttcctca
    38041 tccatagatt ctcctccttt ccgaagaaga caagctagtg actttagctg taatttatca
    38101 taaagtgagg aaataagcga ttacttcatc cccctacaga tttttcgtat aaaaaagaga
    38161 gaatccttat cgacagattc tcctttttaa tcaatttatt ttataaatga aaagttaatt
    38221 taccttttat ggaaaactat tttaaaaaat aaacataaat atgcagttaa tgtaacaaga
    38281 taagaaataa tagtcgttac tgtataactt gtgcttactt taatatcagc aactcctttg
    38341 actccgctta tttgtaccaa tccattagca ttcacctgct cctttacagg ttgatgattt
    38401 atttctacat tcgttcctaa ataagcatat acaggaagtg tgattacttg gctcttctca
    38461 ttagttactc tatatatgat agaactacca gttgttttat aaggaaggtt tacccattga
    38521 ctgccaatct ttgcttcgtg ttttattata tgctccttat tctccaatga agcgatagga
    38581 gaataatcat ataaaaaata atccattcct ttagtggtat acgcctctac tgtttctttg
    38641 tttacaacaa tatgtcccgg ctgagaaatt attttttgat tgaaattttt tttactagaa
    38701 acagttaaag tgattaataa caaaacagtc ataaataaat aaaatagttc tcttgtctta
    38761 ttgtatcttc tatttttgaa aaatatcatc gttaatatca gagaagagaa tattatttgc
    38821 aagcttaaga tccgccaagg aaattgtaac aatttggctg gcgtattttg cagaagatac
    38881 catggcacca aactagttgt agctagccaa gtcaaacacg taccataata aatctttttt
    38941 tctatgtttg ttaatttttt aaataaaatc aggggaatac ataaagataa cagaccaact
    39001 agtcccattg agtaagtagt taagtcgttg ttcatagctt cagtcagact atcgaacaca
    39061 ttagatgcat gagcatataa atttgtcacg ctaggtttgt tgattttttg gtagagcatt
    39121 tgttcaaaca tcggatacca aaaagcactc gtcagaacca atgtcattcc tgccgcacta
    39181 aataattttt taaatatagc aataaaatct gcattcttga aacctttaga aaccaaacga
    39241 ataaaaataa aaatgataat taatgctgta ctcattaatg ttgatagtac atggctgtac
    39301 actagcaaac tcatactcaa tgctagcttt acagacggat acttttcttc ttttccagag
    39361 atgatttgat aagcataaaa taatacaggg ataacaagtg ttattgcgat agcttcggca
    39421 attgcggaac gatagtagat gtctactgtc cgataagcag agaaaatata gagacaacta
    39481 aataaaaatg ccgctttttt ttgttttaaa aaccgctcgc cataatgata agctacgagt
    39541 gttgtacaca agttcaataa ccaaacgtaa ataatatagc ttacaatcaa attttttgtc
    39601 atccaatata acattacagc tggaaaaaaa gtcagaaagg gataaaaata atttacacca
    39661 tatccagtat aattgaatgt ctcataatta atcggactgg taaaaatagt cgataacccc
    39721 ttgattcgag ataagtgaaa agaaagatct ccagtggtat aaatactctg tgtatttttg
    39781 aaaaagatga aagaatatat actactaact ataattaatc cactcaatat tactactttt
    39841 ttatttattt tcattcttct tgtttatctc ctttttagtc acatatcgag aatctttcaa
    39901 cttattaggt gcaaatcgtt taaaacttga atacaccact aaacaataag tcagaatact
    39961 gatccataaa gccatgagag cagttggagt atattttgtt gtaatttcta tcttattttc
    40021 tccagcattt acatccacat tcactaagcc atgtttttta taagagtcat ttacgacttt
    40081 accgtttact tttacctgtg tacctaaata agcatatact ggtactgtca ctttttgctt
    40141 tttgttgctt gccactgtgt aagtaatgtt attatctgca gctttatact tccctttttc
    40201 ccacgattct cctactttta cttcatgttt ttctaagtga ggcctttctt ttatcgcttc
    40261 tgttggggcg taatcgtata atcctcccat atctgatgtt gtataacgtg ggagtgtttc
    40321 ttcattgata acaatatgac cgtgttttga ttggataact tttgcgtaat tttcttttgc
    40381 tgctatagac gtgacgagta gaaacaccac agtagcgcca atgattaacc atgaatattt
    40441 tttattcata gtctttttca taaaaacaag cgttatgatc attgaagaaa aaaagatttg
    40501 gatacctaag atacgccaag gaaactggag caagcttatc ggtgtttttt gaaacaaaaa
    40561 ccatggaaat aaattagtta cgcataccca tgtagaaact acgccaacat agatgatttt
    40621 ttcttttttc tccatttctt tcagtatgaa taaaggaaaa atcaacgcaa ccaatcctaa
    40681 taaccccata gtgtatgttg taatatcgtt atttaacgct tgaatgatac tatctgaaat
    40741 agaaagtgct tctagctgca aatctgtttt gaagggactg tgtatttctt gataattcat
    40801 ctgctgcaac ataggatacc aaaaatagct agttaatgca caagtcaaaa aaacagcctt
    40861 tactaaattc acgaccacaa attttatatc gttttttatt tgcttgctta aaaacaaacg
    40921 aactataatc atacaagcaa tcaatgcact acacaatagt gtagttaaga catgagtata
    40981 cacaagtaga cttatactga cagataaagc gatgactttt ttttctctat tgaatatcaa
    41041 tccataagca taatataaaa cagctgggat aatcgtaaga gcgattgctt ctgcaattgc
    41101 ggatcgataa tagatatcaa ctgttcgata agttgcaaaa gtatataaac aactaaataa
    41161 aaaagcagca tctatctttt taaaaaactt ttctccataa taaaa
//
LOCUS       DACIRY010000009        40607 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.009, whole genome shotgun sequence.
ACCESSION   DACIRY010000009 DACIRY010000000
VERSION     DACIRY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.009"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(45..1928)
                     /locus_tag="IYE22_000382"
     CDS             complement(45..1928)
                     /locus_tag="IYE22_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ9505622.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2194..3069)
                     /locus_tag="IYE22_000383"
     CDS             complement(2194..3069)
                     /locus_tag="IYE22_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9505623.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(3178..4947)
                     /gene="aspS"
                     /locus_tag="IYE22_000384"
     CDS             complement(3178..4947)
                     /gene="aspS"
                     /locus_tag="IYE22_000384"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ9505624.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(4962..6254)
                     /locus_tag="IYE22_000385"
     CDS             complement(4962..6254)
                     /locus_tag="IYE22_000385"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ9505625.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSIPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            6648..7595
                     /locus_tag="IYE22_000386"
     CDS             6648..7595
                     /locus_tag="IYE22_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ9505626.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(7684..8943)
                     /locus_tag="IYE22_000387"
     CDS             complement(7684..8943)
                     /locus_tag="IYE22_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ9505627.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9114..9560)
                     /locus_tag="IYE22_000388"
     CDS             complement(9114..9560)
                     /locus_tag="IYE22_000388"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ9505628.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(9571..11784)
                     /locus_tag="IYE22_000389"
     CDS             complement(9571..11784)
                     /locus_tag="IYE22_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ9505629.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(12017..12781)
                     /locus_tag="IYE22_000390"
     CDS             complement(12017..12781)
                     /locus_tag="IYE22_000390"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ9505630.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(12782..13729)
                     /gene="prmA"
                     /locus_tag="IYE22_000391"
     CDS             complement(12782..13729)
                     /gene="prmA"
                     /locus_tag="IYE22_000391"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ9505631.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(13742..14230)
                     /locus_tag="IYE22_000392"
     CDS             complement(13742..14230)
                     /locus_tag="IYE22_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ9505632.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(14196..14867)
                     /locus_tag="IYE22_000393"
     CDS             complement(14196..14867)
                     /locus_tag="IYE22_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ9505633.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            15005..16291
                     /locus_tag="IYE22_000394"
     CDS             15005..16291
                     /locus_tag="IYE22_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ9505634.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            16363..16557
                     /gene="ssrS"
                     /locus_tag="IYE22_000395"
     ncRNA           16363..16557
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IYE22_000395"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            16635..16919
                     /locus_tag="IYE22_000396"
     CDS             16635..16919
                     /locus_tag="IYE22_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505635.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            16992..17471
                     /locus_tag="IYE22_000397"
     CDS             16992..17471
                     /locus_tag="IYE22_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ9505636.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            17694..17837
                     /locus_tag="IYE22_000398"
     CDS             17694..17837
                     /locus_tag="IYE22_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ9505637.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            18415..19998
                     /locus_tag="IYE22_000399"
     CDS             18415..19998
                     /locus_tag="IYE22_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9505638.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20435..21619)
                     /locus_tag="IYE22_000400"
     CDS             complement(20435..21619)
                     /locus_tag="IYE22_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ9505639.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(21644..22651)
                     /locus_tag="IYE22_000401"
     CDS             complement(21644..22651)
                     /locus_tag="IYE22_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9505640.1"
                     /translation="MLPEKMEQGFNQKLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(22823..23182)
                     /locus_tag="IYE22_000402"
     CDS             complement(22823..23182)
                     /locus_tag="IYE22_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505641.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(23154..23525)
                     /locus_tag="IYE22_000403"
     CDS             complement(23154..23525)
                     /locus_tag="IYE22_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ9505642.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(23542..23844)
                     /locus_tag="IYE22_000404"
     CDS             complement(23542..23844)
                     /locus_tag="IYE22_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505643.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(23861..24331)
                     /locus_tag="IYE22_000405"
     CDS             complement(23861..24331)
                     /locus_tag="IYE22_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ9505644.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(24306..24584)
                     /locus_tag="IYE22_000406"
     CDS             complement(24306..24584)
                     /locus_tag="IYE22_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ9505645.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(24581..25630)
                     /locus_tag="IYE22_000407"
     CDS             complement(24581..25630)
                     /locus_tag="IYE22_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ9505646.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(25587..26516)
                     /gene="cpaF"
                     /locus_tag="IYE22_000408"
     CDS             complement(25587..26516)
                     /gene="cpaF"
                     /locus_tag="IYE22_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ9505647.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            26648..28402
                     /gene="ade"
                     /locus_tag="IYE22_000409"
     CDS             26648..28402
                     /gene="ade"
                     /locus_tag="IYE22_000409"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ9505648.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(28500..29162)
                     /gene="trmB"
                     /locus_tag="IYE22_000410"
     CDS             complement(28500..29162)
                     /gene="trmB"
                     /locus_tag="IYE22_000410"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ9505649.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(29232..30011)
                     /locus_tag="IYE22_000411"
     CDS             complement(29232..30011)
                     /locus_tag="IYE22_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ9505650.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(30218..31429)
                     /locus_tag="IYE22_000412"
     CDS             complement(30218..31429)
                     /locus_tag="IYE22_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9505651.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(31422..32159)
                     /locus_tag="IYE22_000413"
     CDS             complement(31422..32159)
                     /locus_tag="IYE22_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505652.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            32297..32731
                     /locus_tag="IYE22_000414"
     CDS             32297..32731
                     /locus_tag="IYE22_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ9505653.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            32724..33068
                     /locus_tag="IYE22_000415"
     CDS             32724..33068
                     /locus_tag="IYE22_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ9505654.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            33248..34258
                     /locus_tag="IYE22_000416"
     CDS             33248..34258
                     /locus_tag="IYE22_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9505655.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDYLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(34327..35271)
                     /locus_tag="IYE22_000417"
     CDS             complement(34327..35271)
                     /locus_tag="IYE22_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9505656.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(35255..37975)
                     /locus_tag="IYE22_000418"
     CDS             complement(35255..37975)
                     /locus_tag="IYE22_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9505657.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(37972..39192)
                     /locus_tag="IYE22_000419"
     CDS             complement(37972..39192)
                     /locus_tag="IYE22_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ9505658.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(39473..40318)
                     /locus_tag="IYE22_000420"
     CDS             complement(39473..40318)
                     /locus_tag="IYE22_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9505659.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        12020 a         8435 c         7086 g        13066 t
ORIGIN      
        1 ttcaggcaaa aacaaatgtt tttgcctgaa gttttttgta gctatcattc tgtcacgttt
       61 gtctcactgc tgttcgtact tgaacttcct cctagcaagg cttcgctgac taagtgaatg
      121 taatgtgtca tcccttcagg atttggatgg acttgatcat cataaaacca gtcactatgg
      181 tcattactgt agctgtacca atcaatcagc gtcatattgt cgtatttttt cgtcatctgt
      241 tcaagcatcg cattcacgtc attttgccag cgcttcgtcg gtactcggac attgacccag
      301 tatacttttc gatcaccgat tgcatccatc acactatcaa actgagcttc cgagaatgag
      361 ccattcgttc ctagtccgat cagaacagtg tctttcaaca aatcttgttc ctttaatttt
      421 tcaatgtacg gtacactggc atataattgt cgtccgacat ctccatctac cactactttt
      481 ggataaagtt cttgtagatc cgttgcagca tccaacataa ctgaatcacc aaatgccgtt
      541 agttctaatt tttgtgcctt attcccttgt gccttggaca actcgtactt atcaaggatc
      601 gcttggtcaa ccgaactagt ggtgttttct gaattgtttc tggtttcttc tgctaatttt
      661 ttactttcag cgatttttgc ctgcagttgt ttttgatcgg ctgttacagc gtttttcggt
      721 gccgtaacga aaccaacgag tgcaattgct acaagtaatg tgcctggaac aacccatggt
      781 ttgctcttac tgatgatcgg tttgctgaac catcctttca tcacttggaa tgtacgacca
      841 taatcaaaac gtgctagcgg acgctccaca tatcgataag aaagttcact gatgattaga
      901 atcaatgtga tttccactaa agtatgcaac aaaacatgat cggcaatatc agtcaccttt
      961 gcttcataaa aaatcataac tggaaactga tacagataaa tgccatagct tcgtttgcca
     1021 atccaagtaa atactggatt tgtcagccat cgattcaaac ttgctccggg gtgtgctgtc
     1081 actgcaacta gcactacgca taacagactc actaggagca ttcctccgta atagatgaaa
     1141 ttgtaatgat catctaaaaa gaagaagcaa agaatcagtg ccaaaagcga gattcctcca
     1201 gcgatattca gaatattttt cgcttgtttc gggatgtttt tctttaaacg cgtacttggc
     1261 caaacaaacg caagcgcact tcccaaccat atagaaaaca gacgagtgtc ggtaccgtaa
     1321 taaacacggg ttggatcgcc tcctggtgta tacaagatca tcatcaagat ggcggagagg
     1381 aaggatgtac ctaacacagt ataaaagatc cattttcttt tacgaacaaa taccatcaac
     1441 aatacaaaca agatcggcca aaccaaatag ttttgacctt ctactgctaa agaccagata
     1501 tgcgtaaacg gtgactcatt agcaaatcga tcgaaataag ataaaccgtt attgatttgc
     1561 caccagttgt tgacatacag taagctgctg accacaactc ctcgaagatt gtttaacagc
     1621 cctcgctgaa acaaagtgat ataagccgaa gctgtcacaa gcataaaaac aagtgccgga
     1681 tagagtctct tcattcttct gacataaaat tgccaaatat tgatttttcc attttgttcc
     1741 cattcctgcc gcagcagatc cgtgatcaaa taaccagata cgacgaaaaa aatcggaacg
     1801 cccaaatagc cgcctttcat actagttggc agaaggtgat agaagataac acccaccaca
     1861 gctaatgaac ggagcccatc aaacccagtg atgtagcggc tgttttttaa ccttttttgt
     1921 ttttccattc aatattccaa cttttcataa acttttttca ttcgtaatat acgggtatta
     1981 agtgaaaata gcaagtagaa tcgtgtcctt tctctactat ttttttctat catcccataa
     2041 atttaaaaaa ataaacatgc tttttttcaa ctttaagaaa atttcaaata aaaaagcagt
     2101 ttttctttgc tgattctact atggtagaaa aaaactgcag acaaacgaac gtctgtctac
     2161 agcagtattt catgaatttt tgtcaaactc gatctaaatg ccatcctcat agaaacgacc
     2221 aagtatctgg acgttttctg caatacttac aaaagcttgt ggatcagaag ctttcattgc
     2281 ttctctaagt agaggcagtt cgtatcgtgt aacgatcgtc atcaatactg tctgcttatc
     2341 atgcttataa gcaccttctg cttcattgat gatcgtgatc cctctacgca tacagttttg
     2401 gatttcattt actacttttt ctggatgttt cgttacgatc attacctgca tcttcttttg
     2461 tttagtataa acgacatcag ttacttttcc actgacgaaa atagacaacg tactgtaaaa
     2521 catatattgc cagccaaata gaattccggc aacaaaaacg atcaatgcat tgaagtagat
     2581 cgaaatagaa ccaatcgact ttcctgtttt tttacgaatc gtgatactca cgatatctag
     2641 accgcccgat gataggccgt ttctcaacgc tagcccaata cctagtccca tcacagcccc
     2701 cccaaacaac gcacagatga ttggatcggt agataggact gtttccggta aaatatggat
     2761 acaaagggag gtcaaagtga ctgtgataaa agtgaaaatt gtaaacttct tcccgatttt
     2821 taaccacgcc aatacaaaca aaggtatatt cagtaaatac aaagttgttg ataccggtac
     2881 ttcgaatcct atcagtgatt ttgacaaagt cgtcaaaatc tgagctagac cagtaatacc
     2941 acttgaataa atatgtcctg gctgataaaa gaaattcaat gcgactgatg cgatcaacgc
     3001 ataaacgata gataccgaaa gcttggtcgt gtaatcatga taagcccaat tttcgaatgt
     3061 ttttttcatg gaaaagcccc gctttatatg ttatggcttc attataaagt gtttttcaat
     3121 taaatcaagg ttgtcaaaag aagcgtattc gacaaatcct ttttaccttt ttatcggcta
     3181 ttcttctata ttcgttacat caatattcaa ttcgaacaat tgtaaaggcg aaaccatact
     3241 tggtgcatca ctcatcacat ctgctgcttt tccgttttta ggaaaggcaa tgacttcacg
     3301 aatattatct tctccggcta ataacattgc aaaacggtcc aatcctaatg caataccgcc
     3361 atgtggaggg aatccgtaat ctaaagctgt taacaagaaa ccaaattgtt cttccatttg
     3421 ttcttttgta aagcctagag tttctagtac tttttcttga agctcacgag tatgaatacg
     3481 gatagaacct ccgcctagtt cataaccgtt caagacgata tcataagctt ctgcatagac
     3541 ttttgcaggg tcttcagcca ataacgggat atcttcttct tttggcatcg tgaatggatg
     3601 gtgagcagag acatatcgac cttcttcttc tgaaaattca aattgtggcc aatcagttac
     3661 ccataagaaa ttgaatttcg attcatcaat tagtcctaat tcttttccta aacgtgtccg
     3721 gatagcacct aatgcagcag cgacgatttc acttttatct gcgccaaaca tcagcaagtc
     3781 acctggtttt gcatctgttg ctttaatgat tgcttctgtt gcttctccca tgaatttagc
     3841 aattggacct ttcaatccgt cttcttctac tttcagccat gcaagcccct tagctccgaa
     3901 ttgtcctaca tattgaccta actgatccat gtcttttcgt gagtagcgat ctgcagcgcc
     3961 tttagcattc aatgctttca cgacaccgcc gttttcaaga gccatttgga agactttgaa
     4021 ttctacttct ttgactgtat cgcttagatc gatcaattcc atatcaaaac gtgtatccgg
     4081 tttgtcgctg ccgtagcgag ccattgcttc atcataggtc atacgaggaa atggcaatgt
     4141 gacttcgata ccgcgaactt ctttcattac cttcgcaatc aatccctctg tgtaagtttg
     4201 gatttcttct gctgttaaga acgttgtttc aatatctact tgcgtaaatt caggctgacg
     4261 gtctccacgt aaatcttcat cgcggaaaca acgaacgatt tgataataac ggtcgaatcc
     4321 agcattcatc agcaattgtt taaacaattg aggagattgc ggcaacgcat agaaatgtcc
     4381 agcatggaca cgtgaaggaa ctaggtagtc tcttgcgcct tctggtgtag atttccctag
     4441 gtaaggtgtt tcgatatcga gaaagtcatt atcatctaaa taatgacgaa tcgcttttgt
     4501 tgtcgcattt cttaatttga tgtttttagt catttttgga cgacgcagat ccaaataacg
     4561 atatttcaaa cgtatttcgt cactgattgc ttgatcatct tcaatttgaa atggcggggt
     4621 ttttgcttga ttcaaaatca cgatttcact tgcaatcact tcgaattcac cagtggccat
     4681 tttaggattt actgcatttt ctccgcgtct tctaacttcc ccagtcactt cgatcacgta
     4741 ttcactgcga catttatccg cgatttccca tgcttctttg ttgatttcag gattaaagac
     4801 gatttgaacg attccttcac gatcacgtag gtcgataaag attactcccc caaggtctcg
     4861 tcttttttgt acccatcctt ttaatgtaac tgtttgttct aattgttgtt cggaaactaa
     4921 tccgcaatag atcgttcttt gtgccattta tttttttcct cctactcgaa taaagtcatt
     4981 tcatcataga cttcatcaaa atgttcatat atctcttcta atggaaaact tttttctttt
     5041 cggcttgcca ttgctttgac attaatcgtt tgactagcaa gctcatcttc accgatcacc
     5101 aatactaatt tggcattcgc tttatctgct gtcttaaatt gcgcttttgc tttacggttc
     5161 atgaagtcgc ggtcagctga aaaaccaaag tttcgaatcg cttggaccac tttcaatgct
     5221 tcgatatttg tttcgtctcc gagtgcgacc acgtaagcat ccaactcatt aaatgcaggg
     5281 ataactactt cctctgcctc catagtcaag agtaatcgct cgatacccat tgcaaatccg
     5341 aagccaggta ttgaaggacc acctagttct tccactagat tgtcataacg tccgcctgcg
     5401 caaatcgttg cttgtgcccc catttttggt gcgtcactca tgatttcaaa aatcgtatgt
     5461 gtgtaataat ctagtccgcg aaccatatta ctgtcgattt cataaggaac agccaatgca
     5521 tccagcatgg aaacaacggt atcaaaatgt ttttgagcag gctcgcttag ataatctaag
     5581 atagatggag cttctgccac aaatttttga tctcgtttgt ctttactgtc caatacgcgt
     5641 aatggatttt catgaaggcg gcgtttagag tcttcactca gctctgattc aaaaggtaaa
     5701 agatagtcga tcaatgcttg acgataggct tgacgcgttt ctttatcacc taatgaattg
     5761 atgactagcc ggatttgcgt gatccccaac tgtttgaaga aatcaagtgc catcaccatg
     5821 ctctccacat cagttgcagg gttttctgat ccaaatgctt ctactccgat ttgatggaat
     5881 tgacgtaacc gtcctttttg agggcgttcg tagcgaaaca ttggtcccat ataaaaagtt
     5941 ttgaacggtt tcgcatactc tgggccgaac aatttatgct caacaaaaga acgaacgatt
     6001 ggtgctgtac cttctggtcg aagcgtcaca tggcgttccc ctttgtcata aaaatcatac
     6061 atctcttttg aaacgatatc cgttgtatcc cctacacttc gggatatcac ttcatagtgt
     6121 tcaaaaatcg gtgtacgcat ttcattatac tgataatctc tgaataataa acgtgctgtt
     6181 tcttcgacaa actgccattt ttctgattcg cctggcaaaa tatcatttgt tccttttggt
     6241 ctttggaaac tcatagtttt ctctcccttt cttagctatg taaataaaaa aatcaccctt
     6301 gttcatcatg aacaagggtg aatgaaattc atacacggta ccacctaatt ttgaaagatt
     6361 tctctttcct cttacgaata acgctcgtac gcgggacggc ttttcactcg tccatctcca
     6421 gagtgtcttt aggctaatga aactttagat cattttcagc ctcgatgatc ctctcttgta
     6481 aagtttcaaa aacttactcg ctctttcatt gacattcgat attacttata acctactaaa
     6541 aaccagtctc aaagtcaaga gatatctgac tatttcccct cacaaaaacg ttttagttca
     6601 gctttttggt attttgtttt agactagaca aaaaggagga atcagctatg gcagaaataa
     6661 gaatcggaca ttcacaagtc tacgcagaac agcttggatt aggagcaaat gccgttggag
     6721 gacataatct ttttgacggt ttggaagatg agacaggaaa acaagtagtt cgtacagctt
     6781 tgaatagcgg gatcaatttg atcgataccg cttatgctta cggaaacggc cgttccgaag
     6841 aactgattgg cgaggtactg aaggaaaaag aatatgatcg ctcacgtgtc gtcatcgcta
     6901 caaaagctgc tcatgtacca aataaagggc gaacatttga taattctcct gagtttctca
     6961 aacaatcggt ggaagatgca ctaaaacgtc tgcaaactga ttacatcgac attttttata
     7021 tccactttcc ggatgaaagc acaccaaaaa atgaatcagt tgctacattg cacgaattaa
     7081 aagaagcagg aaaaatccga gcagtcggtg tgtcaaactt cacgttagaa caattgaaag
     7141 aagcgaatgc agatggatat gtcgatgtag tagaagataa atacagtttg atccatcggc
     7201 aggctgaaaa agaattattc ccttatcttg aaaaaaacaa aatcagtttt gttccctatt
     7261 ttcctcttgc ttccggtttg ctgacaggaa aatatgaact tggtgaggaa aaacaatttg
     7321 gtgaaggaga tcctcgaaaa agaaatccag atttccaagg agaacgtttc agagaaattt
     7381 taaccgctgt cgatgtactt cgccccattg ctaaaagata ccaagccact cctgcgcaac
     7441 ttgttttagc ttggtacatg aagaatccac gagtgtctgt agttatacct ggagctaagc
     7501 gtccagagca agtatctgac aatgtccaag ctttagattt gcatttatcc aatgaagatt
     7561 atcaaaccat tgatgaagca tttcgtggat tttgatcaat tgaccttatc aatttatagt
     7621 aatatgaaaa aaggaagatc agcactgttt gatcttcctt tttttcgata agtttatgct
     7681 aatctaattc ttgtaaagta gcgctcggct attcggataa gtcggaaact gattgaatgg
     7741 aatcgtccaa tcttgttcag gtacagagaa ctcaaactta tttactagct gatcggaaaa
     7801 cacacgtaga ctatgaagag tgatccattc tcctgcacag cgatgcattt gtcgaaaatt
     7861 tcccccgcct tgtgcaatca tctcatattc ttctttatag gaaatatctt ttgctttccc
     7921 tacataccgt ttgatcataa aggaatctgg cgcttcaact gttcgttcat catggtctgt
     7981 tccatataaa tctaaaataa cccaactgcc ttctggtata cgataaccat ctacttctac
     8041 ttctttcagc gaaatcgcag gtagcattgg gaagaaagga taatagcgtc tcatttcttg
     8101 aataaacgga tcttgtaacg tcgaaaagtc attttttaat tgctgataca aatctggtct
     8161 actgaaaagt gcatgtccca tcaatgccgc ccacacagtt agagcaaccg ttggacggat
     8221 gatgttcaat aattcaacag ctgccacttc cagaggtaaa agctgaccat ctaaatctgt
     8281 ggcattggca aaagcatata atgccacatt ttcttttcca gccacaggat tttgtcgagc
     8341 ttcttcaatc aagccttgcg cccatttttc tgattttttg cgattttcga caccttttat
     8401 gtgatcaatc ggagaagtaa acgttccgct gatcatagag atttgatatt ctgctaattt
     8461 ttcgacctca tctttagaaa tcgctgataa attgatacct gcccactcgc aaatagatgt
     8521 aaagaaaacc atcttactta aatcaaatag ttcaaattga ccatgctgtg cttcaagtgc
     8581 ttgtgtcaga ttcttatcta agatacgatg ataatcttcc attctttccg gtgtcattag
     8641 atccataaaa atatttttcc gatggtgatg agcagctcca tccaaagtct gtactccacc
     8701 ttgaccaaaa agtgtcttca atactaattt tggcatagca ccttttcgtt tgaaattcct
     8761 tggatcataa aatacttttg ctgctgatga tccgtagatc gtgatagctt ctttattgaa
     8821 aatctttgct ttgacgacgg gagcgtccgc ttcatggcgt aactcctcca acatgttgta
     8881 tcctttttga tacaattttt ttaaatctgt gattttgata tctactactg gtacttcttt
     8941 catcttcagc aacctcctat aaaaaataac ttgtataatt taagcttaac tttttttccg
     9001 actgctgaca aaagaagcgc tttttgcaga caaaaaagcc acttaagtta gagtttatca
     9061 gtctaactta agcgacgtgt ttcatagttt ttactagtga aaaccagatt actttatttg
     9121 tttttagaat caataatgat cgttacaggt ccatcattga cgagagaaac cgacatatct
     9181 gctccgaatt gtccggtagc gactgtgatg ccgcgacttc tgattccttc attgaatgat
     9241 tcgtataaag gaatagctgt ttctggtcgt gcggcttcca caaagcttgg tcgatttcct
     9301 tttttcgtat ccgcaaacag tgtgaattga gagatactta aaatttgtcc tcccacatct
     9361 tctacactaa tattcatttt tccttgttca tcttcaaaaa tacgcatttg tgcaattttt
     9421 tttaccagat agtccacatc tttttgcgta tcggtttcat gaatgcctaa taaaacaagt
     9481 aggccgtagc caatttttcc aatttcttgc tgatcgactg acacagcagc tttacttacc
     9541 cgttgaacaa ctgctctcat atctgtcctc ctatccattc gtccggcgta cactgtagac
     9601 atcaggtact gctttgattt tatccacgat actctttagg tgggataaat tctggatacc
     9661 aaccgtcaaa tggatcgtcg ccattttatc tttattcgtc cgtgcttcaa cgctgttgag
     9721 atttttcgtt agcgcattga ctgtttgaag tacatcattc aataagcctg aacgattata
     9781 gccgtaaatt tccaaatctg catcatactc ttttcgtgta ttcgatgtat cttcccattc
     9841 gacttcaatc aaacgttctg ctacatttgg tttgtcaggc tgaacattcg gacaatctcg
     9901 acgatgaatg gatatccctc tgcctttagt gatataacca acgatatcat caccaggaat
     9961 cggattacag cagcgactga tacgaattag taagttttct accccttgaa tgacaacgcc
    10021 accttcatga cgtactttca ttttgtcagg ttcttttttc ggctgattca tgatttcttc
    10081 tgcttcttgc ttttgctgct cgattttttg ttctttacgt tctttttcag tcagacgatt
    10141 ggccatcgtc aaggggctaa cttctccata ccctacagct gcatagagat catcttctgt
    10201 ttgataatta aaacgatcga gtgcttcttg cagtttgttc ttcgtcaaaa tatctttagg
    10261 cgtaaatcct agatctgtaa tacatttgac cacggattcg tggcctttga tcacattttc
    10321 ttctcgatct tgggctttga agaaacgttt gatcttattt cttgctttgc tagtagcaac
    10381 taatttcaac cagtcgcgac ttgggccaaa tgaatttgga gaagtcatga tctcaatgat
    10441 atctccgttt ttcaatttgt aatcaagctg caccattttg ccatttactt ttgcaccagt
    10501 ggttttgtta ccgatatctg tatggatact gtatgcaaag tccaatggtc cggatccttt
    10561 tggtaactca gtaacgtctc cttttggtgt gaagacgtag actttatcac taaagatatc
    10621 tccttttacg ccttccataa attcagaagc atcatagctt tcgtcttgaa gttcgagtat
    10681 ctcatggaac cagcttaatt gtttcgtcat accatctggt tctacttttt cattttttcc
    10741 ttctttatag gcccagtgtg cagcaacccc gaattcagcg atttcatgca tttcctgtgt
    10801 tcggatttga atttctaccg gatttcctgc cggaccgatc acggtagtat gcaaagattg
    10861 gtacatatta gcttttggca tggcaatata atccttaaat ctgccgggca ttggtttcca
    10921 ttttgtgtgg attgctccta atacagcata acaatccttg attgaatcta cgatcactcg
    10981 aatagctagc aagtcataaa tttcgttgaa ctgctttttc tgatctttca ttttacgata
    11041 gattgaataa atgtgttttg gccgtccata gatttctgca aaaatcccca gctcttctgt
    11101 cgctattcga atatcttcaa cagtaccact tacgtatttt tcacgttctt ctctcttcgt
    11161 ctgcatcaag tggacgatac ggtaatactg ttttggattt agataacgaa gtgctgtatc
    11221 ttctaattcc catttgatcc ggctgatccc tagacgatgt gcaagaggcg catagatttc
    11281 taaagtttcc tgagcgatcc ttctttgctt atcttctcgc aggtgcttca acgtacgcat
    11341 gttatgtagt ctatcagcta atttgaccat gataactcgt aaatcttgag ccattgctag
    11401 cagcatcttt cggtggtttt ctgctagctg ctcttcgtgg gatttatatt ttattttccc
    11461 taatttggtt acgccgtcca ctaacatagc aacgtcatcg ccaaattctt ctctcagatc
    11521 ttctaaagtg atttctgtat cttcaacaac atcatgtaag aagcctgtag ccactgtatg
    11581 gggatccatt tttaaatctg ctaatatacc tgcgacttgg ataggatgaa taatgtaggg
    11641 ttcgcctgat tttctaaact gtccgtcatg tgcagcagtc gcgtattcgc atgctttctc
    11701 aacaaatgct acatgttcgg gacccatgta ttgactaacg agctttatga caccgggtcc
    11761 cgttaaaatt tcttcttttg tcacaggaat cgatccccct ttcttttttc ggtaaaacgg
    11821 gaatcgggac aaatatctgt ttgataattg tcccaaaatc cgtgcagtta tacgtttttt
    11881 tacggaaatg ttcgcttttt aaaagctttt tcaaacgatt tttcgtatcc attgtataaa
    11941 taattgtttc tattatacaa attgttgact attttttcaa ttctttctgt cgatactctt
    12001 ttattaatga agaacgttag ctgaaagaag gattcattaa ttcccattga tagctagcag
    12061 cacttaataa ataaagaggt gctgtttctg tacgaaggat tcttggtcct aatccacaaa
    12121 gtatcccttt gtgttctaca aaataatcga cttcatcgcg ggataatcca ccttctggtc
    12181 caaagacagc taagattcga ctatctggtt ttgtttcact aagaattttg gctgcatttg
    12241 atttttcgcc ttgctttgct gattcctcat aagcaaccaa gaccacgtca taatcatcta
    12301 gccgctcaaa taaatcttgt ttcgtttcca ataactctac tgatggcacg atctgccgat
    12361 gggattgttc cgctgcctct tgagcaatct tttcaagccg ttgttgtcgt ttcatacgtt
    12421 tttttgcatc ccatttaacg acagaggctg atgccgggaa accaagaaaa gcttgcgctc
    12481 ctaattcagt ggatttctga acgatccaat ccagcttttc ccctttagga tagccactcg
    12541 ctatggtaat cgaacttggc agttcctttt gattttcttc ttttttaatt tctttcagat
    12601 aaacagcctc ttcagtaatc tccgtgattt ctgcaacaat ggctatttga tcattgaatg
    12661 ccaaaaaaac ttcatcctcg ggttccatcc tcataacccg tacaatgtgg tgatagtttt
    12721 ccccttctaa aacaaaatag tcttttgatt catatgattc ttttaaaaaa taccgctgca
    12781 tctattccac ctctggtttt ttcaaaataa ttgcatacca gtctttttgc tggaaaattt
    12841 gatccacgat aaacccttgt tctaacatgg catccagaac catttgcttc ttctcttcaa
    12901 tgattcctga aacgatcagc gtgcctgttt gcttcagtaa acgccaagca tctgggatca
    12961 tcaataagat gatatctgct aaaatgttcg caacgattac atcactttca atctcgattc
    13021 ctgtcaaaag atcgttagct gatacatgga catctttggc tatttcattc atatccatat
    13081 tttctttcgc cgaacgaacg gctacttcgt ctaaatcaaa ggcatagaca tcttttgcac
    13141 caagatattt tgctgcaata cttagcacgc ccgacccagt tcccacatct agcaatgttt
    13201 caccaccacg caagactgtt tctagtgctg tcaatgtcaa ggaagtcgtg ggatgagtgc
    13261 ctgtgccaaa tgccatacca ggatctagtg tgataatttt ttctagagga tcttctgtct
    13321 ggtagttttc ccagcttggc gcaaccgtca gcattcgcgt aatacgcacc ggatgatagt
    13381 atttcttcca ggctgttgcc cagttgcttt cctctacttc actaactgtt actttatttt
    13441 ttccaatagc tagaccaaac tcaggtaatt gtgtgatacg atctttgata aaaggcaaga
    13501 tttccggtaa gaaagtcgtt tccggaaaat aagccatgac tagtgcgcct tctttcagcg
    13561 atgtaaaatc atttttatct aaaatctcac catattgatc cgactgaaaa tgttcaacat
    13621 ctaaagaatc ttcaatcgcg acaccactcg cccctgcctc catcaaaata ttagcaactg
    13681 cttcgactgc ttcactttca gtttctactt ttacttcatt ccatttcata tctgtcactc
    13741 cttaataact tgggtacggt aaataagtat ctgcttcatt ttgctgtttt acttctacta
    13801 attctgctag ttgctgatta tcccagtgaa gctcgaatac agcttcgtca tcgtcttcat
    13861 ccaaaaaatc aagcaaatcg ctttgccctt gagctaatac ttcatttaga tattccacta
    13921 aagaatcagc tactgatttt gccaaccctt tttttccttc ataagggatt actgctagat
    13981 aattgcgttc atcaaaaaca gatttctgag gattaaaaag aagtacgccg tcttcaaatt
    14041 cgataattgg ttcttgcgaa acgatccctt ctgcatcatc aatctcaaat tgtgctttat
    14101 tttctgcaaa caagcgaaca acgatctcga tcgtatgatt ttttgtatcc caatctaaag
    14161 ctacatcata gtctgtcact tgtttttcaa ttatttcatc taaatatgtt aatagatcct
    14221 tctttttcat tttctttcct ccaaccccaa tctttctcga tttggttttt tctttgtaaa
    14281 gagatataag gatttcccga cacaacaaaa cgcaacggct tttctgtcca tacacctttg
    14341 tttgggatac cgattctagg caaagcattg atcttttttg gtgtcttttt tttctcaaaa
    14401 actaagcgta acgaactatc tgctatagac tgaccataaa gttcttgagg aattgctaat
    14461 gcttccacta attttcccgg accgttgctg atatctggac cagtttttcc gccacgattt
    14521 ttactcattt gatcaatcat tgcggcgggt tcgattgcac gtatcattac tccttgagga
    14581 atcccttctg gctgagtaat gatgttcaaa atccgatgag tatgcatcgt gtaaaggtag
    14641 atcgtacctg gtttttcata cattgcccgc acacgcggtg ttcttcttaa accatagcta
    14701 tgggcggcct cgtcttctgg acctaggtat gcttccgcgt ctacaatata tccagctaat
    14761 cttccttctg gtgtgtcatg ctccaaatac atccccaata aatcccgtgc cacttctggc
    14821 gtcgttttat tccaaaaaaa ttcatctatc ttttgtttat ccgtcatttt tctcacctac
    14881 tttatcatac acaatattag ttggataagc tatgaaaaca aaacaacaga aggctgttct
    14941 ttctaggcat cttgctaaat atgatataat taagggacgt aaaaacaaga aggaggaaaa
    15001 tcctatgcaa cagccattgg cttatcgcat gagacctcga acgatcgatg aagtcgttgg
    15061 tcagcagcat ctagtcgggg aaggaaaaat catacgccgc atggtcgatg ctcgcatgct
    15121 ctcttccatg attctatatg gacctcctgg tactggaaaa acgagtatcg caagtgcgat
    15181 cgccggctct actaattatg catttcgcat gctaaacgcc gctacagatt caaaaaagga
    15241 tctacaagtt gtagcggaag aagcaaaaat gagtggtaca gtgatcctct tattggatga
    15301 agtacaccga ttagataaaa caaaacagga ttttctgctt cctcatttag aaagtggccg
    15361 aatcatcttg atcggagcga ctactgagaa tccttatatt accatcaatc cagcgatccg
    15421 cagccgtaca cagatctttg aagtcaaacc attaacagaa caagacatcc agttggcagt
    15481 cgaacatgct ttaagggata aagaacgcgg ccttggacaa caagcaatcc agttagacga
    15541 agatgctctt cttcaccttt cgcgtgcaac gaacggcgat ttgcggagtg cattgaatgg
    15601 gctggaactg gctacactat cgactccttc agataaagaa ggaaggattc atcttacact
    15661 gtcgatcatt gaagaatgtg tccaaagaaa agctcttacc catgacaaaa atggagatgc
    15721 tcattatgac gttatttcgg cttttcaaaa atcgattcgt ggaagtgatg tggatgctgc
    15781 acttcattat ctcgcccgtt tagttgaggc aggtgatctg gcaagtattt gccggcgtct
    15841 gatggttatc ggttatgaag atataggttt aggtaatcct gctgctgcgg ctcgtacagt
    15901 gaacgctgta ttagctgctg aaaaattggg attgcctgag gcacgtatcc cactagcaga
    15961 cgtcgtcgtc gatctttgct tgtcgccaaa atccaattct gcctacatgg cattagatgc
    16021 agcacttgct gatattcgag agggcaaagc tggagatgtt cctgatcatt tgagagatag
    16081 ccattacaaa ggtgcgaaat cactcaaccg tggcgtgggt tatcagtatc ctcatcattt
    16141 tgatcaggct tgggtcaatc agcaatattt accagataaa ctgaagaatg ctcagtatta
    16201 tcagccaaaa gatacaggga aatatgaaca ggctctgggt caacagtatt accgtataaa
    16261 agagtggaaa aagcatcctc caaaaaacta gacaagaaaa cggttctgtt tctgcgagct
    16321 ctctcattgc tctttttaaa ttcatatgct actatagagg tgggaaatct gtgatgttcg
    16381 aattgtcctc tttgtgtgtt gaccgaacat ttttattgat atcttgggac ccgtcttgtg
    16441 tcactgctgg taacatgcct tatcatttgg tagttcgaag ctacgaccat gggacccacc
    16501 tgcttgaatt cgcgggatca atactacagg acaaacaacg gcattaacgg agattaccat
    16561 cttttaaaga ataaagcaat ttgctcttgg caaattgctt tttctatttg ttgcttattt
    16621 tggaggattt gttcatgatt aggattttaa tgattatcgc cactttagta ttattattcg
    16681 ttagctatta tttatttaat aaacaggata tcttttttgt attgatcaac aaaaatgata
    16741 aaaatcaagg attcctacaa ttttacggag ctgcatatgc tgttttaggc gtaatgggta
    16801 ttttaacagc ttttttcaat caacgcttta ttgccttggt ctttttactg atcgtaatta
    16861 ttgtttctgc caccttcagt atccgatttg caaaaaaaat tgctgaacca aagcagtaaa
    16921 taattctctt tttccctaaa gtgttctaca ataaaagagt acaatacgat tgacttgagg
    16981 agtgaatgct tatgttacaa caatacaaaa aaatcatggt tgccgtagat ggttctgatg
    17041 aagctgaatt agcttttaaa aaggcggtca atgtagctat ccgaaataac ggagaacttc
    17101 tattagcaca tgtcattgac acacgttctt tccaaaccgt ttcatccttt gatggaatgc
    17161 ttgcagaaca agctactgaa atggcaaagc aaacattggc agattatgaa agcaatgcga
    17221 agaaagctgg cttgaataat gtaacctctg tcgtagaata tggctcacca aaacaaatta
    17281 ttgccagaga aatcccagaa gataatcaag tagatttgat tatgttagga gccacaggat
    17341 tgaatgctgt agaacgttta tttattggtt ctgtttctga atatgttatt agaaacgcag
    17401 cttgcgatgt gcttgttgtc cgaactgatt tagaaaacca attgccagct aaagaccaca
    17461 ccaaagctta acacgtagac gacctgccaa ctatcggcag gtttttttgc acaaaaaacc
    17521 gtgataaaaa caaaagacgc ggcaagcact catcggtctt tataatgttt aaaataaagg
    17581 gaaaacagtc aatgagttca agtaaaataa aaaaaagtga acaccagggc gattagtcgc
    17641 cctggtgtgg tttaaaacga cctaagcatg ccaaagctta ttggttcgtc aatgtggaag
    17701 tttccgatga atctgatacg aaaaaatatg aagtatcgat ggcggatacc gattttatcg
    17761 ccaaactaaa attttatttc gggttcgggt accgcgagaa ggcaagtgct acattcggta
    17821 aatcattcaa gaattgatga aaggctaccg cttgccattg caaccacaac gatactcctg
    17881 aacgcgatgt tggtgagatt tccaaagaga agagctgttt aatgcaacgg tattaagcaa
    17941 atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa caccatatga tttcgcctga
    18001 acgctggttt agctcagaat acaatgatgt ggtccacgag acattaccct cagtcagata
    18061 aaaaaagctc tctgacagaa ttacacatcg ataaatatta agtaaatgtc agcgctagtt
    18121 gaactttctg acactttcag gtacactgga aaagacaact acacgaagga gggaaaatac
    18181 tatgatgcgc atgactaaaa taacaagtaa tgaaaattac caacgatacc atatcgcatt
    18241 tagccatgca attggtttta cagttgctgt tgacgggaca agtttgtcca aaacagttga
    18301 attcacgtaa aatcaatggc catctcaagg tatttttctc atgataatct gagtttaaat
    18361 aacacaggcc atggataacc atggcctttt tgagtggtct caaggagaga gatcatgatc
    18421 atacaagcaa tttctattaa aaaaacaatc ggcgggaatc cattatttga ggatttatcc
    18481 tgtcaaattg aagctggtga aaaaattgcc ttagtcggta tcaacggaac tggcaaatca
    18541 acctttttac aaatcttgac cggcagagaa ggtgtcgaca gcggtgttat cagtcgcaaa
    18601 aaaggcttaa agatcgggac ggtcgaacaa gaacttacag tttccgaagc gaccgtcaac
    18661 cactacttgc tccattctgc cgctgagata caggacctca aacagcaaat gagtcgctac
    18721 gaagcattga tgacagaacc agatacaaac ttagagaagt gtctggcccg ttacggtgag
    18781 ctgcagcatc gctttgaaga actaggcggt tacgtattag aagaccggat tgcagcgatc
    18841 ttgcaaggct tagggattcc acaccgtcag gaagcacggt taacagaact tagcggaggc
    18901 gaacgagtta aagtcgcttt agccaaaatt ttagcagccg atgccgattt acttttactc
    18961 gatgaaccga ccaatcattt agatcttaac agtattcgct ggttggaaaa ctatttacag
    19021 aatacaaaaa aatcggttct ggtcgtttct catgatcggc aatttctcga tcaagtcacc
    19081 acaaaaacat gggaactgga agaaggtgca ttgattgagt atcccggaaa ctacagccgt
    19141 tttcgagtct tgaaagaagc tcgcttggct gagctgacaa aaaattacga actgcagcaa
    19201 aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc gtcagtgggc acatgaagga
    19261 gacaatgagt ccttcttcaa aaaagccaaa gaactggaac gccgcctggc gaaactgaca
    19321 ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa gcttgtctaa tggtggaaaa
    19381 tctggcaaag aagttttcat cattcaaaac ttgcaccagc agtacgctga tcaagtacta
    19441 ttcaaagaca gcagttttgc agtctatcgt ggagatcatt tagccatcat cggcgacaat
    19501 ggtgcaggaa aatcaacctt actcaaatta ttactcggtg aggaaaagcc ttacggtggc
    19561 accatcaaat taggtagtag cttgcaaatc ggttatttgc cgcagcagct gcagtttgcc
    19621 gatcccgata gccgtttgct ggcttacgcc atcacattga ctggaaacga agaagtcgct
    19681 cgacggcaat tagccaaaag tggcttttat caaagcgatg tgggtaaacg gatcaaagat
    19741 ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac tctttttaga aaaaatcaac
    19801 gtcctgattt tggatgaacc aaccaatcac ctcgacagct atgctcgaga agaaattgaa
    19861 gaaatgctgc aagactatca aggaacgctc ctagcagtca gccatgaccg ctactttttg
    19921 cagcaacact tccaagaagc actcgttatt gatcaggagc agatcagccg ccagccgctc
    19981 ggccaactga ttgactagga cgttttttcg caacaaaaaa cttgattttc atacctgtgc
    20041 tttcacgatt caggcaaagt acaggtggaa aatcaagttt ttattattcg atcaatgaat
    20101 gtttcggatc ggttttctag ctaaacataa agcgaagaat aaagtcgaca gcccacgagg
    20161 ctgatctaaa aatgtttttt ctttttacat atgtaatgtc ccttataaag atgaatgaaa
    20221 ataaaatatt gggattttcc tgaaaatcaa taaataaaat gccagtcttt tcccttttga
    20281 ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa aaagaaggga aattgaagtt
    20341 aaattaccat tttgcggtct tctccgcaaa aaaaacacat gcaagaattc cctcttgcat
    20401 gcgtttcatc ttttatccta acgaatgctt tggcttagcc gcgcagacgc tccacatcac
    20461 gggcaatcat tagttcctca tctgttggga tcactaatac tcttactttt gattcttttg
    20521 tagagatatc tacttcttca ccacgcaatt cattcaacgc tggatcgatt tcacaaccaa
    20581 accaagacat acctttgatg atttcactac gtacatgtcc gtcattctcg ccgataccag
    20641 cagtaaagac aattgcatca acaccattca tcgttgtcac ataaccgccg atgtattttc
    20701 ggatgcggtc cacgaaaatg tcataagcta aacgaatatg ttctttttca tagttctttt
    20761 caagatcacg catatcacta gacaatcctg ttaggcctag cagaccagat ttcttgttca
    20821 aaacattgat catttcatca atatcaatct ctaatttttc cattaaatat gggagaacag
    20881 ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc agctaatggt gtgaatccca
    20941 tagaagtatc catggattta cctccatcga ctgctgtgat cgatacacca tttcctaagt
    21001 ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa taattttgcc gcacgttccg
    21061 acacataacg atgacttgtg ccatggaagc catattttct tactttgaaa tcttcataat
    21121 aagacaccgg taagctatat aaataatttt gctttggcat tgttgagtgg aaagatgtat
    21181 cgaatacagc cacactcaaa atgtcaggta aaatcttttt aaacgctttg atccccatta
    21241 agtttgcagg attatgcaaa ggagcaaatt ctgctaattc agcaatgcgg tccattactt
    21301 tctcatcgat cactactgaa tccccataag tttctcctcc gtgaacaaca cggtgcccta
    21361 ctccggtgat ttcatcataa gagcttaaaa tttgcagttc aatcaactga tctaacagca
    21421 tttttactgc tacttcgtgg ttttcgatat ccatgatttg ttcgaatttt tgattatcgc
    21481 cgtacttgat ggtaaaaata gaatcttgta aaccgatacg ttcgacgatc cctttcgcaa
    21541 ctacggtttc ttctggcatt tgatataatt gccatttcaa acttgaactc ccagcattga
    21601 ttgcaattgt tttagacata tgactctcct tctcgttgaa tgattacaga gcagatgatt
    21661 tccagttttt aaactcgttg aaaaattccg tcaccttctc tggttctttt aatgaagcaa
    21721 gtttcacaag caagacttct tttacttgtt gtgcttgtgg tccttttttc tgtaagatca
    21781 aaatactttt ttgcgattgt ttattacgga acaattcatc tggcaattga atcattcctt
    21841 gaagataacc ttcttcttta aaccattttt tgatttcttc actttgatcc gtttctaaaa
    21901 atccactcgg catcaaaaac aaaccaaaac catccggctt cacatatttc attgattgct
    21961 ctagcaataa atgatgtgca taactatggc cttcttcggt acttgttaaa aactcttttg
    22021 ctttttcgtc attcggataa taaccgattg gcaaatcgct gattgcaaaa tcgacaggat
    22081 ctattaataa ttcctgcaac ccgtcttgat gaaagtattg aacatttgct tgtgtcaaat
    22141 cacttgtaga agctgcgaca gccaacaatg tatcatcaat atccacacca attccttctg
    22201 tttggtaccc cgcattcgat aggtttaaaa gaactgttaa taacaagttg cccattcctg
    22261 ccgcaatatc cagaatctta accggtgctt ttttatttgt gtatagttgt tcaataagaa
    22321 aaacaaataa aaaaccgata ctatcaggag tgagttgatg gttcgcctgc aaatgttctg
    22381 tctggctgcc cttcaataaa agtaattgag acagtctgcg ccattcttct ggctcgaaag
    22441 aaagtttctt caactcttca taaagagctg tgatccgttg cgttgtctct tttgtcggca
    22501 ctccatccac cacacgtact tgataatcat ctattaaatt ttctgcattt tcgacatagg
    22561 cttctaaaaa ggaagttccc aatgcattct gtaaaagctg aatagcttcc agtttttgat
    22621 taaacccttg ttccattttt tctggtaaca tggtgcacct catttctaga tatattataa
    22681 caattttttc acctgtttaa ttctatcgaa caattgacaa aatttcaatc gttttcactg
    22741 ttttttattt gtgaaacggc aaacattttt ttattaaaaa cagtgttata acagaaaaga
    22801 gggcgttttc acgaacattc catcattctg tactagtatc tgtatcattt tttacttctt
    22861 ctgtgaaccg aaagataaac ggtgagactt ccacctctaa gaatagggac tgttgtaccc
    22921 actgatatgt tacagatcca gttgtgtagt aaaaaactcc ttccgaactt tcggcaagcg
    22981 tagactgttc cgataaagcc atctgcttca ttatttttgc tatataataa tttttgattc
    23041 gtatattaag atccattgac aaacggtagc tttctaatgc tgccatcaat aaaaacgtac
    23101 ataaaagaaa taaaagcaag acagaaaata aaatagagcc cttataacat tttctactga
    23161 tcgttttttg tccatatggc atatccatct tctccatttt caaagtgtac ataaaaagaa
    23221 attgtgtgtt ctttttccaa taattgaagc tcagtgatac ttgttaacat aggttgatgt
    23281 ccgccggaag ttcttctttt acgtatcatt gcctgatatc tttcgatgat atactgttta
    23341 tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga cattgtgact gctcttgagt
    23401 tcttcttcta attgtgctaa aaatattagc cactcttttt gatctttacg ctgcaaaaat
    23461 tcacttactt ggatggtttg ctgaatcaaa agattgaatg tgagcaatag actgctcaat
    23521 atcatcagtc caattagaca ttcaatcagc gtaaatgctg gaattacttg ttttttcaat
    23581 actgatcgct tctttctcca tattgacaaa cactttttcg attactccat tattatcgac
    23641 aatttggata tcgtatggga tcatttcttc ccttcgctct ggtcttttgt gatacatccg
    23701 ctgcgtcttg atatcttcat aaatcattcg aataaatagc agttcctccc cacttttctt
    23761 ttctgcctga agcaaaaaca gctggccaaa gctcactaag tagacacaga cagaagcgat
    23821 cataagcgct gtaagggact ccaacaaaac aaatcctgtt ttattcaatt ctttttgtat
    23881 agcgaccact ccctatctgg aattgatacg taatcgtccg ctctttttct tcccaataaa
    23941 attgataagc ttttagtgaa ctttcattcc cggttctagc agcaaaagta atagaagcat
    24001 ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc ccaatttatt ttatctggat
    24061 tcggaacatg aaaaatgatt tgattctctt cactattcca ataaaagcct gtttggatct
    24121 gattcacaat agcgattttt tgcgtagaat agattcgttt ttcaaattgg ccgaaaaatt
    24181 gatagactgc caattcattc ttccatgcag aaaatgcgag aactggaaaa gaaaggatcg
    24241 ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt atatcctgat gctaaaacct
    24301 taccttcaag gccgctgata gatttctgca tgttctcgtt tgatatagcc ttcattcaac
    24361 aattcctcca cagtaggaac tcttccatta ttttgaagcg catagagctc tttttgtgtg
    24421 tctaccaact ggatgatcgc ttcacgactt tcttggtcta catgattcct ataacgcgac
    24481 aaattaggga cgaacaacaa cactaataca ctgatgatca gtaagacgat tagcatttcg
    24541 atcaatgtaa aaccgctata ataagatatt tttctctttt tcatggcaaa acctcctcta
    24601 tgcttccgta aacaggtaat aaaacagcgg cataaactaa taaaatcaaa gccgctacgc
    24661 ccaaaaaaat gattggctga ataaaatgca tccagtgatg aagacgtttg aaaagctgtt
    24721 ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa acttcccttt gcctctccct
    24781 gtaaaacaat tttactgaat tcttctgata gaaaacgata attctggaac tgctcagcca
    24841 atgacatacc tgcctccaat ccttgaatca acttagaggc aagatgctga atcagacttt
    24901 cttgtttcgt ttcctttaag cagactatga tttgccttaa ttcgaaacct tcatgaaaaa
    24961 gctttccaat ctctagtgac aaataagccg attgatacaa ggaatatatc ggtccaatca
    25021 ataattgtct gcttatccat tcactctgat caacaggaga caacttcatc agacgatacc
    25081 ttactagtaa agccaataga gtaaccgcta ggatgaatag tagaacatac caatgaaaga
    25141 cttgtatcag ctgaataccc caatgctctt gcatgaccat tcctgaacca ataagctgag
    25201 gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa aaaggtgagc aatagcatcg
    25261 gatatgcaag cagctttttc aaatcattgc gaaatccttc aacaagccga aactgtttgg
    25321 caatttccaa caaagttgtc ggcagattcc catgacgttc agctaactcg atctgtacca
    25381 gttgctctgc tccatatcct atttgcgaaa gtacctcact aaacgcttgc ccttccttta
    25441 gctgatgttg gatatttgcc aaaataacag gtggaaacga acggatcgtg agtaataaat
    25501 aaagggattc ttgcagacta aaaccagata aaagcagttc tcccatggtt tgtgcaaact
    25561 ggttttgctg gcttcttgtt aatcctttag aatagttctg actgccaaat attttcctga
    25621 gcttcttcat aactttcctc ccatgatttt tttactgatt cttcctccat aaattgataa
    25681 ctccataaaa cagaaggatg gtgattggca tcttccaaaa gttcttgata aatgacacca
    25741 gctaaacatt cagatagctc ttcttttttc tttgtcagtt catggattct gaccaatgta
    25801 ccttcaagat tgcgtgcatg tacggttgca aaaacccggt gccctgttag tgctgctctt
    25861 accgcagcat gtgcagttga ttcatctctt atctcgccaa tgattagcaa gtctggacga
    25921 tgccgtaatg cgagtctcaa aagtgtatca taagtctgtg ctattttcgg attgatctgt
    25981 agctgcagaa aaaacggttc ttctatctct acaggatctt cgatagtaat cacttgcaat
    26041 ttttcttcca aagccaaccg atacattaga gaagtcttgc ctgatccgac tggtccgctg
    26101 aaaaggtata atccacgtct gacggttttc tgtaaaatag ttgcaaaatc tcgagaacta
    26161 aagcaacgat ggggtgtttc aaaagtaccg tataatagcc gaatcactaa actctctcgt
    26221 tgaaggtaat ctcctacaga agacaaccgc agacgaactt cctgtttttc taaagaataa
    26281 gtaaatgcac ctaactgagc ttttcttttt tctccgatat ccatctgtcc caaatactta
    26341 aatcttgaga tcagttgctg tccatctgcc gaagagactt tcttccagaa tgttcgtttt
    26401 tttccatagc gaaaatagat gtaaaatact tctgaagctg gcaatatata tacgtcttgg
    26461 gcatgtttgg caaaactttc acttatcatt tgatccgata atcgttttat ttccattatt
    26521 ctcccctcca caaaaaacat acgtcttttt gatctttttg tttcaaataa aatgaattta
    26581 caaaaaaata ttggcaaaaa ccgaatttat gttatactta taaaaattta tcttgggagg
    26641 cactccattg gaaacaacat taaggaaata tatcgaagta gcaagtaaaa aaaagcctgc
    26701 tgacctagtg atcaagcatg ctaagatcgt caacgtcttt acgaaagaaa tcatggaaaa
    26761 agatgtagca atctgcgaag gaatgatcgt aggaatcggt gaatacgaag gaaaaagcgt
    26821 acataatgct aatgggcaat atctagtgcc aggattcatc gatggccatg tgcatatcga
    26881 aagctcactt ttatcaccaa gcgaattttc aaaagtatcc ttgcttcatg gagttactac
    26941 tgtagtgaca gatccccatg aaataggaaa cattgctggg agtacaggtc ttgagtttat
    27001 gatagaagat gcaagacaaa cactcatgaa tatctttttt atgctacctt cttgtgtacc
    27061 tgtaacccct tttgagacca atggtgcaaa tttaaatgca aaaaaattag cccctttttt
    27121 acaaaaaccc gaagttttag gcttagctga agtcatgaac tatcaagcag tagcaaataa
    27181 tgaaacagac atcctagaaa agatccaatt gatgcaccag cataagaaaa aaattgacgg
    27241 gcatgcagct ggtatcggca tggaagagtt aaatgtttat cctgcagcag gtattcggac
    27301 ggatcatgaa gctacaactg caaaagaagc taaagaacgt ttggatcttg gtatgtattt
    27361 aatggttcga gaagggaccg ttgcaaaaga tctggcatcg ctactaccgg cgatcacacc
    27421 tgaaaattca agaagatgct tctttgtcac cgatgataaa ctgatcaatg atttagtaaa
    27481 agaaggatca atcgatcata tcgtccgtca agctattgca cttggaatcg atccattaca
    27541 agccattcag atggcaacat tgaatgcagc agaatgtttt gatttgaaaa cactaggtgc
    27601 gattgcccct gggtatcaag ctgatttctt cttaacagac gatcttacca agctgccaat
    27661 tcatgaagtt ttctataagg gaacattagt tgttcaatct gggcaactcc aagaccattt
    27721 atttgctgat cattcaaatt catatacgtg ttgtctgcca aaactaaacg ctcaaccatt
    27781 aaatccacat tcgttagagc tgcttctttc ttcttcacaa gcccatgtga tcgaaattat
    27841 cccgaatagt ctagtgacta aagatttggt agaaaaagtg gaccgacaag gcgaacgatt
    27901 cattccttca atcgaaaaag accaattaaa gattgcggtg atcgagcgtc accatcaaac
    27961 gggaaacatt ggattaggca tagtgaaagg atttcagctg aaacaaggag cgatagcgac
    28021 aacgattgcc catgactcgc ataatctagt tgtcgttggg acaaacgacg aggatatgct
    28081 gtatgctgct aatcttttga ttcaaaaagg tggcgggatg accgttgtca acaaacagca
    28141 ggaaatagct tgtgtttctc ttcctatagg cggaatcatg acacaggaac ctttttcgat
    28201 cgtctatgaa cagttgaatg aattaataac taaagcacat caactaggag cagctaaaac
    28261 ctttgatcct tttttgacac tttctttctt aacattatct gtcatacccg aattgaagat
    28321 caccgacaaa ggattatttt cattttctcg cttccaattg attccacctt gtggaaaaac
    28381 cgatgaaata aatacatttt aaatcaaata aatcccagaa aatacattac atctgtaatg
    28441 ttcattttct gggatttatt tatggatatt aatgttttta tgaaagcaga aagttagttt
    28501 cacagacttt tctttttggt cttatcttga aattgagctt ctactcgata aatacgttga
    28561 ccttttgaag aaaatttttc ttcatactcc gtcataatat tgccttcaaa ttgactttga
    28621 tgtaaatcta accatacctg tttgaggatc atgccatatt gagaaaaact tgccagagaa
    28681 tactcaaaca atccttgatt atctgttttg aaatggattt ctccatttgg acgaagaatc
    28741 tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc gtttttcatg acgttttttt
    28801 ggccatggat cggaaaaatt cagatagatt tgatctactt cgctgtcagc gaaatactct
    28861 gtcaacgctg atccatccac atgtagaagt ttcaggttag gaagcggttg ttcgatcaac
    28921 ttgtctagtg caatcgaaac aacactgacc tgcatttcta ctccaatata attgatttca
    28981 ggatgggctt ttgccattcc agtgatgaac tggcctttcc ccataccaat ctcaatgtgg
    29041 attggatgat cgttttcaaa caattcattc cattttccct tccacaatgt tggatcacta
    29101 attacaaaat tcgggtgagc cgctaacatt tccgctgctc ccggacgatt tcggacacgc
    29161 attatttatc tccattcctt tcctctaatt aaaaaggtct ggccaaatct ccgcagagtt
    29221 tgggccaaac ctcagccact gaaaattcgt tttaattgta aaatttcttc gttcatccgt
    29281 cgatgatctt cacgtttgca atatctggtg atttcttgga gcaaattcat ggccgcatac
    29341 caatgaaatt tttcaagcat ttcgtcagtc gggcgtatgc cataactgat cagccactga
    29401 ttccagctag acagtggcac ataatttcct aaaatcgttg caatatccac cgcagggtct
    29461 gcaaacatga ttgaatccca atcgactaga tataaataat ttttacatac taaccaatta
    29521 cgatggttta catctccgtg tactactgat aagttttcct tatctattga cgggatctgc
    29581 tcacgaagaa aacgatggac aatttgcaga aattgatttt tctggagttc ttccggtaat
    29641 tctttttcat atttttgcag catcttctcc ggcgtcatga cacgaccacc gatacgggtc
    29701 agcatggttt taagagaatt tgaatgatgt aaatggtaca aaacatcaat cacgtcgttt
    29761 ctttgaccaa tctcattagg agtcagcaag cgaccttcca gccactcttg tgctgtcaaa
    29821 gtatcgccat tccccgttcg ttttgtccaa acaagttttg gtgtaattcc ttcccttgaa
    29881 agagcagcca acatcggagt cgtgtttcgt ttaataaaga ctttgtcctc atcacgcatg
    29941 cctatgtatg tttttcctgt gtcgcctttg atgggacgta accgccactc ttggtctaac
    30001 tgaaactcca tcatgcattc tcccgtcctc ttactctttg ttttgcagta cttattattc
    30061 tagtcgcctg aacactacac gtcaagcaca tatacgttct ttttcattta attgccattc
    30121 aaaaaaccag cttttctcaa aaaaaataga acgaacagca ttcttactgt caacgctcta
    30181 tttttcattc tcatttttac cctacacagc tttgttttca gtctgacatc ttcttcaaac
    30241 gagttggtac atatagttta acaaagagca tagtaacaat caaataagcc gctagcggaa
    30301 cccatcgctg ctgcccatct aatacgactg cagcaatcaa accaaaaata accgacgcga
    30361 taattaatac ccatgtgagt agatactgaa tcgcctgctt cttctgattc ttatggattg
    30421 gataaagctg aacgagaacc atatagcgga actggttcgc taaaggtaac agttggaaac
    30481 cgatcaaata taaaaataag aaaccgataa tcagactaaa ataaaagtcg actactccgg
    30541 caatcaataa cccacctata attactaatc ggaaataaag accactaaaa tcagaacctc
    30601 ttgccaatct tcttgcaaac aagtaaagat aagtgttttt tgattctctt ttaatatgag
    30661 tcaatatagg atccaaatag cgtcttcttt tgatctttgc cgtaatttca ggaacatctg
    30721 tgaataaatt gataaattga tagatctgat gcaacctatg ttcttctaca tgcaccattt
    30781 tctcccaatc caatcctttt tcttgttgct tccacaaaaa ccagtaatat agacttgcct
    30841 gtactaatgc acctgctatt cctgcccata ttagccaata aaatgataga aacaaaaaga
    30901 tcagagaaga aaaaaaccag cctaatttcc aataacgaat aacttgttga tctagtcgaa
    30961 acaattctgc tctcttgatt tgcagatgac tgaatttcaa actccataat atgatcagat
    31021 agacgaaaaa catcgaaaag gattgctccg tactaaccac cactaatggc atggccaatc
    31081 cgcatgccaa tatcaaaaag atcaacggaa aaatgcaaga atagcgaaaa gctcgttcca
    31141 aataggtttg catttctttt tctttaggaa gtaaaaaaac aatatctgct gcctctgcaa
    31201 aagtagccat cttacccacg aataaacaga gcatccaaaa tacagagatt atgagccctc
    31261 caattgcaaa cggtggctgc agggttttca accaattaga ataatataat cccacaccgc
    31321 caatcaagaa aagacaaact aaaacaaaat gatcgttcaa tacataacgc atatatttca
    31381 tcattttctt ttgatgtctt gctaaacgtt tttgaaaaaa actactcatg tgcctgctcc
    31441 tttgttaaag caatatagat ttcgtccaaa gaagaatccg gcagctgaaa ttcttttctc
    31501 aacatctcaa gagaaccgtc tgctcgtatc tctccgttat gtaagaccac aaaacgatca
    31561 caatattttt ctgcagtagc taaaatatgc gtagacatca aaataccggc tccttctttt
    31621 ttcatttcta ccatcaattc aagcaatgca ttgatagcta gtggatccaa gcctaaaaat
    31681 ggttcgtcga tgatatataa agaaggctcg atcaaaaagg cgcataagat catcactttc
    31741 tgtttcattc cctttgaaaa attagctggg aaccagtcaa gacgattaga taaccgaaaa
    31801 gtctccaaca acttttctgc tcttttcatt gctatatcca taggtatatc gtaggccatc
    31861 gcagtgactt cgatatgttc tctcaatgtc aattcttcat ataaagaagg agtctctgga
    31921 atatacccaa tttgtttgcg atattcttcc gtatttttct ttaaggatag atgattgatt
    31981 tctatcgttc cttttgaagc ttccagcaga ccgataatgt gcttgatggt cgtacttttc
    32041 cctgctccgt tcagtccaat caaccctact atctctccag gctgtacatc gaaactgata
    32101 tcttttaaca cagggagatg tccgtacccc cctgtgacat gatcgataat taaactcatt
    32161 ctttttcctc cgccatttat ttttatttat tataccacga agaaaatgac ggtattttgt
    32221 ttttttcctt acaatagctt taacttatgg taaagttaag ctgaagtatt tattctttat
    32281 agaaaggatg ttttatatgg aaaactgtat tttttgcaaa atcatcaatc aagaaatccc
    32341 aagctataaa atatatgaag acgacaaggt ctatgctttt ttagacatct ctcaagcaac
    32401 gaaaggacac acattagttg tgcctaaacg tcatgtagca gatatttttg aatatgatcc
    32461 cgaacttgca ggagaggttt tttcgcgtgt gcctaagatt gctcaagcat tggaaaaagc
    32521 ttttccagaa atgaaaggtc ttaatatctt gaataacaac cgtgaactag cctatcaatc
    32581 tgtttttcat tcacatatcc atttagtccc tcgctacagc aaagaggatg atttttctat
    32641 tcactttgtc aatcatcaag atagttatgg atcggaagaa ctaaaagcta ttcaagaaac
    32701 aatcgtaaag caggtgtcct gcgatgatta aaaacttttt caaaggactg ttatttggag
    32761 ctgctgctgg cactgtcgga ggtctgttat tggcgcccag gagcgggaat gaaacaagaa
    32821 agaaactgat cgaagaattg gaagaagcaa caactctgac aaatgattta aataacagcc
    32881 tgaatcattt taaaagcgca ttgatcgaaa caaaagaaac agctgaatct gtgattcctg
    32941 ctttccaaga agcaatcaaa aaagatatcg aagattttaa attccaagcc gaaccacgta
    33001 tcacacaaat caatgaacaa gtagaaaaac taaaagctga tctgccagag tatcctgaac
    33061 aaatataata atgattattt tctcaacaga tttaactata taagtgagaa aagtttgcgt
    33121 atttcctagg aatgtgatga aattttgaat tttgtttgaa actaccacga caacgttttt
    33181 aaaggaaatg ggatatggta tagttaatat ataaaaataa taactaatta caggagtgca
    33241 aattttaatg aaaaaaaaat caattatttt ggcagctact agtgcgttgg ctgttttaac
    33301 tctagcagct tgttcaggcg atactaataa agatatcgca acgatgaaag gcggaacgat
    33361 cactgtatct gatttctatg atgaagcaaa acttgaatca tcgaaccaat ccttagtaca
    33421 acggatgatc atttataaag tattcaataa caaatatggc gataaagtaa ccgataagca
    33481 agtggatgca gaatacgaca aacaagcgaa atcattaggt gatacttttg aatcacaatt
    33541 agaagctgct ggatatacga aagatactta taaagaatat attcgtaaca atttagcttt
    33601 tgaagctggc ttgaaagctc acgtagatat tacagatgac tatttgaaaa ctgcttggaa
    33661 atctttccat ccagaagtag aagcacaaat catcaaatta tcaagtgaag atgaagcaaa
    33721 agatgtgaaa aaatcagcgg atgatggaga tgatttctct aaattagcca aagacaaatc
    33781 aactgataca gagacaaaag aagatggcgg taaagttaaa ttcgactcaa cgacaacaac
    33841 tattcctgca gaagtaaaag aagcagcttt caaattgaaa gacggtgaga tctccgatgt
    33901 tattactaca acaaatccta cttcttatgc gacagaatac tacgttgtca aaatggtgaa
    33961 aaaccaaaat aaaggcaatg acatggataa atacaaagat caactaaaag atatcgctac
    34021 tgaaactaaa ctaagtgaca atgcattcac aactaaagta atcggtgaag aattaaaaga
    34081 tgcaaatgta aaaatcaaag atgatgcctt tgaaaatgtg ttgagtgcat tcacaacaac
    34141 ttcatcaagt acgaaagact caagtgaaac tacagcttca acaaagagtt ctgacacaaa
    34201 atcaactgat tcaacaaaag aatcttcaac tgaagaaaca actgattcat caaaataaaa
    34261 gaaaacaaaa aaagcgaaga cttatgtctc gctttttttg ttttgaatga aaaaaataaa
    34321 tttttgctat aaatcttttg gtacatagaa acttcggttg tccataccga aaatccgatc
    34381 ggtgtatttt cctggttctg tttgacgaat cgaagtcaac atcatctgca tcgaagcatc
    34441 gatattgtcg atttgatgta atatttctgc ttccattatt cttggacgta caggtgagcc
    34501 atattctaat aagccatggt gtgctaaaac catatgacgt aatacgagaa catcttcgtc
    34561 agcttcgctg attttcaacg caagacaggc tttagttatt tcttcgtcaa tcaataccag
    34621 atgaccgatc aaatttcctg caagcgtata ttctgtcgac ataggaccgg aaagttcaat
    34681 cacctttcct aaatcatgca aaatgatacc agcatacaac aatgctcgat tcagctcagg
    34741 atattcatcg gcgattgatt tccctaagtg aagcattgtc aaagtatgat aagctaatcc
    34801 actggcaaat gcatgatggt ttctttttgc tgccggaaac tcaaaaaatt gtttttgata
    34861 tttattaagt agatagcgaa caatacgatt ccaatgcgca ttggtgatct caaacaacaa
    34921 ttgattgatt tcttcttcca tttcttcttt cttcaaaggg gctctttcca tgtataaact
    34981 aggctctcct ggctcatcta cccttgttaa gcgcatatga agaatcttta cttgagggtt
    35041 tccttgataa acctctcttt taccgtttag aaaaacgact gtgccggctg taaatttatt
    35101 gatttcctcc tccgaagcat cccagtattt accatcgata gtacctgaag tatcttggaa
    35161 agtaaaagca ataaattttt ttccgttttt agcgattctt acatctgcac ttttgatcag
    35221 gacaaattgt tcaaactgct catcgattac taaatcacga atttttttca ttatttgctc
    35281 cttctaattg ttttacgatt tggttcatct catggtaata gcttaccatt tctttatcag
    35341 aagaaaaaca aattatttga tacttttctc caaatttagc taaaagttca gctaattgat
    35401 actttcgctg atgatcataa tgaagccagc catcatcaat gatgatcggg catatcgaac
    35461 ggttagcttc taatgagagg taagcaaatc tcatcgccat aatcagctga tctttcgtac
    35521 ctgtagataa ctcatagatg ggaaattctg tatatccgtc tgttaaagtc agtgtatcat
    35581 ttttcagtaa tatcttctga taatttcctt ttgttaaaat acctaaataa tcacctgctg
    35641 ctcttaacaa ctggggtaat tgtctttcac tcatctctgt tgaaagatca cctaagaatg
    35701 tcccggcgat ttcaagtgct ccccattcct ccagtaattc ctgtaatttt gctttttgcc
    35761 ggctagcttg ttgataaagt tcgtccaatg tgccgtctct ttgcaactct tctacttgaa
    35821 gttggagtcg ttgccgattt tcacttgctt ctttttcttc ttgctggagt tgggattgct
    35881 tttctttcag ttggcgcagt cttacagata atttctcttt tgtcagctct tcaggaaata
    35941 gtggagtaag catttccgat aattcattgt atcttttcat ctgctgttcg gcttgtttgg
    36001 ctttttgtaa aaagacaggt acttccgaag gataagataa gcctgcttga gtcaattgtt
    36061 cgctgtattc ttgtatccat actttttgtt tctttttcaa ttgattgatt tcttgcttca
    36121 ataaagtatt ttgctgatag cttctggcaa atttcatctg ttccatctct tgtataaaac
    36181 gggaaagcaa taccattttt tctgaaattg tcttatcttg tatcggtaac cattctttaa
    36241 caaactcaaa tttttggtcg atttgtcgta gtttttcttg tatttcctgt tctcttttat
    36301 gtttccgctc aaatacgcca tataatttct gataatctat gacttcctgt ccatattctt
    36361 tcatgagttt gagcgtatcc atctttccta agtgccgatg atccgcaaaa cgatatactt
    36421 cttgctgaag aatctgtttt tcttctccag ttctcttttg tttttcttct gcttcagcta
    36481 attgagctaa ggctagatca agttgtccta gtttttcttg ccacattcct tttatctcag
    36541 agaaatcatt tttagtttta atttttggtt gccttttgta gaagaaatag ccaatcatgc
    36601 ctgcaccaat tagtaaaagg atcactttca agggcattgg tacaaatata cctgcaatga
    36661 ttattgctcc gccagccatc agcatatacg gtttagatga agagactggt gctatctctg
    36721 actgattttg taataatgct ggataggtct cttccaagtc attcacttct gcttcaatca
    36781 tctcttgctg ttctttagct atttggagac gcacagaaat ctggctttct agatcagcaa
    36841 tccttctttc ttcagccaga agatgcttcc attcttcttc atataaaaat tcttccggta
    36901 acggattttt ctgccaatgc cacctttgtt ccaaaatctt caattctgaa tttgtttctc
    36961 tagtctcaat ttgtaatctt tgccattctt cgtttaattg ttccgctgta aattgaagtt
    37021 gctggagatc ttttagttcc tgctcctgct ctaaataaaa ataatatctg gctgactgct
    37081 gatccatccc gctatgcttt tccagtgttg cttctaattc ttccaaacgc tcatttgttt
    37141 gctgataccg tttggagaaa gactccagat ctaaaagaag ttgctcatca ggaagacctg
    37201 tagtgctgtt ttttttgaga gattgccatt cttcatataa agaaaaatta agttcttgct
    37261 gtctggctaa tgaaaattgt ttgttcgcct cttttaattg tgccaataac tgctgctgtt
    37321 gcgactcgta ttcctgttct tgttggatca acttgccaaa ataagcttcc tgttcttgtt
    37381 tttgatgaat tttttcttgc agctctttcc attcatttaa tttttgattg attagtaatt
    37441 tttggccttt tttcttgaat aattgttgtg ctttctgata atattcttgc cgcttttgga
    37501 atacttgaga actgcccgtg atgcctaaag aaatcagcgc atcatgtagc tctttttctc
    37561 tcaaatgatc tagttccatc aactgctctt gctggaaagt aaatacctgc tggaataact
    37621 ctttcgtcaa aggtgcgatc agtttttcta gtaactcatc acttcccact tgatccccca
    37681 gccacacctt cgattttccg cgattttgct gcttgtagcg ctcgacagca aattcaccat
    37741 atacaggatg tttaagccat attttgccgc cataggctga gccgtctttt ggtgtataat
    37801 cctttttttt acggcctttt gaagggaaac caaataatat tgcttgaata aattgataaa
    37861 tcgttgattt tcctgcttcg tttcccccat agatcagctg attgcctgaa taaaagtcaa
    37921 aacttttttg acgccatttt ccaaaagcag caatctcgat ccttaaaatc ttcattcctg
    37981 atcctcctca aataaaaaat cttgattaag cagctgcttg gctttttcaa gtgtttcttc
    38041 ttgatattcc ggcagttcgc tcaatattct agccgcttct tgatgactat atacttcatt
    38101 taaaatttgt tggaaatctt gaggtgtctg atagtattga aataactgtt ccatcaattt
    38161 agcagaagca gcaagtgggg tcttcgtata aaaagtattc tctatgagag aaatacgcca
    38221 aataaagaaa tcatttatcc tatctgagaa ttcctctaac aaatagtcta atagctctcc
    38281 tgattgtatc cgttccaaaa catctgtatt gagatggtca taatctttta attttatctc
    38341 aattaaacta aatccatcct ctacttttga aagctgctga cgaatgtgaa caatgacctc
    38401 ttttgtcgtt cttgctttcc tcaatgatat ttcctttgtc ttccaataga cctctgctac
    38461 tttaatcgga tgtatttggc aacttccttt acttaattca actagtagaa tagatgtaga
    38521 agcttgttcc tttttagtat gaccttgagg cgcacctgga taaacaattg tttgcttttc
    38581 atttaagacc gttggcacat ggatatgccc taaagcccaa taatcgtatc ccttttcttg
    38641 catctttgag ggttggaaag gagcatagtt tcctttttga gagacgccag gttccccgtg
    38701 gtacaaccca atatggtaat ctgtgagttc tctagaagga aattccatca ctttgtcttt
    38761 ttgaatccac ggttgacgat aactaaatcc actgagagag acagtttctc cactttttat
    38821 cgttattttt tttgtctcta ccgtttcgga tgtaaataaa tggacatttt taggaaagga
    38881 gaaccaatac cgctcttttt gatagaaatc atgatttccg aatattataa atacaggtat
    38941 atggttcttt tctaaccgtt tcatctgttc tgaaaaatgt ttctgtattt ttaatgaagg
    39001 acgattttga tgaaaattat ctcctgcaag taaaacaaaa tcaacgttat ttattattgc
    39061 ctgttcaatg atatttgata aaacggtcag attcgtcttc aatagttttt cctgtacagt
    39121 tttatcaaga gttgtcagtc cttcaaatga ccgatccata tgtaaatctg ccgcatggat
    39181 aaatttcacc attaaaaatt tcctcctttt tttcttttat cataccattt tcaatgtttt
    39241 ttttcatcaa taaccgaaaa tttgttcgta ttatgcaaag aaattacgat aaatggccga
    39301 cttcaaaaaa taagtttatt cgaaaaaaag attgtgacat gatttttgtc acaatcctaa
    39361 atatctatgt tatattcgct tcgtttgaca ctgaagaagt actatttgta ctggaattta
    39421 tgctccattt ttccgtattt agaattctat gttctatact accttaatta gtttatatgg
    39481 taagaattct ttcaaatgct tgagatactc cgtctttatc atttgaaaca gtgacataat
    39541 cagctttttt catgatttct tgatgactat ttcccattgc aaaactaata tctgcacttt
    39601 caaacataga taagtcattt aaactatctc cgatcgcgat tctcaaaggt ttctttaaat
    39661 tgtagattga ttccaaacga tcaaacacca cacctttaga tacattttga ggtacgattt
    39721 caatatttac aggtgccgat tcagtaacag acacatttag catctttttc aaatggttaa
    39781 atatattagc tttcaattga cgattaggaa taaaaataaa aaatttgtaa atgctgatag
    39841 catccttttc tataatttcc tcaatattat tataagggat tgaacaaata gatttttcat
    39901 actcaagctc atagtttagc tctttgatag aaaattcact ccctacctcg tgaaaaattt
    39961 ttttcatttt atttttataa ttctgagatt cataacttcc gagatttgta tatattttgg
    40021 ttggaaattt cgataggaca gaatcaattt tttttacatc atttttatcg aaggaagact
    40081 caaatatctt tttttctcca tcaaatccaa cagaaccatt taaccctact gctggtatcc
    40141 ttaaaccaga ttttttgaga atattattaa tgtcttctac ttctcttcca gaacaaatga
    40201 atggaaaaca gtcttccggt aaatgtttca ttgttcttgc atttttttga ctaatgctct
    40261 ggtcagaatt caaaagagta ccgtctaaat ctataccaat tagtttcatt tcatccatgt
    40321 ctcctatacg ttgattataa attcactgct tattgtaagt ctaaatttct cacaaaacat
    40381 atactaatta tacctaattt aagtaagtac aaacatcatt ttaagaatag gaaaggacat
    40441 ttatatacta ttctctgtat ttttgattta taaataaata tgaattgaat ccaaatcagc
    40501 tatattacta ttttataata accaactata aaaaacgaag ttaggtgaaa aactaacttc
    40561 gatattcata aaagactcta ttagttcaac ttagcaaaag atttaaa
//
LOCUS       DACIRY010000010        39756 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.010, whole genome shotgun sequence.
ACCESSION   DACIRY010000010 DACIRY010000000
VERSION     DACIRY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.010"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(5..586)
                     /locus_tag="IYE22_000421"
     CDS             complement(5..586)
                     /locus_tag="IYE22_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9505660.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IYE22_000422"
     CDS             complement(769..1395)
                     /locus_tag="IYE22_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9505661.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IYE22_000423"
     CDS             complement(1417..2748)
                     /locus_tag="IYE22_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ9505662.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IYE22_000424"
     CDS             complement(2903..3304)
                     /locus_tag="IYE22_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ9505663.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IYE22_000425"
     CDS             complement(3390..3596)
                     /locus_tag="IYE22_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505664.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IYE22_000426"
     CDS             complement(3691..5193)
                     /locus_tag="IYE22_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ9505665.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IYE22_000427"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IYE22_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ9505666.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IYE22_000428"
     CDS             complement(5764..6381)
                     /locus_tag="IYE22_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505667.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IYE22_000429"
     CDS             6602..7975
                     /locus_tag="IYE22_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ9505668.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IYE22_000430"
     CDS             8050..9162
                     /locus_tag="IYE22_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ9505669.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IYE22_000431"
     CDS             complement(9234..10871)
                     /locus_tag="IYE22_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ9505670.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IYE22_000432"
     CDS             complement(11039..11647)
                     /locus_tag="IYE22_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9505671.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IYE22_000433"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IYE22_000433"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ9505672.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IYE22_000434"
     CDS             complement(13201..13953)
                     /locus_tag="IYE22_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505673.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IYE22_000435"
     CDS             complement(14094..15044)
                     /locus_tag="IYE22_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ9505674.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IYE22_000436"
     CDS             complement(15045..15884)
                     /locus_tag="IYE22_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ9505675.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IYE22_000437"
     CDS             complement(15881..16627)
                     /locus_tag="IYE22_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505676.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IYE22_000438"
     CDS             16865..18235
                     /locus_tag="IYE22_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9505677.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IYE22_000439"
     CDS             complement(18290..19186)
                     /locus_tag="IYE22_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9505678.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IYE22_000440"
     CDS             complement(19467..20144)
                     /locus_tag="IYE22_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ9505679.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IYE22_000441"
     CDS             complement(20235..21140)
                     /locus_tag="IYE22_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9505680.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IYE22_000442"
     CDS             21315..22769
                     /locus_tag="IYE22_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9505681.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IYE22_000443"
     CDS             22785..23816
                     /locus_tag="IYE22_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ9505682.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IYE22_000444"
     CDS             23823..25010
                     /locus_tag="IYE22_000444"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ9505683.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IYE22_000445"
     CDS             25166..26548
                     /locus_tag="IYE22_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ9505684.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26675..26905
                     /locus_tag="IYE22_000446"
     CDS             26675..26905
                     /locus_tag="IYE22_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505685.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27105..27773)
                     /locus_tag="IYE22_000447"
     CDS             complement(27105..27773)
                     /locus_tag="IYE22_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ9505686.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(27962..28759)
                     /locus_tag="IYE22_000448"
                     /pseudo
     CDS             complement(27962..28759)
                     /locus_tag="IYE22_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(29040..30419)
                     /locus_tag="IYE22_000449"
     CDS             complement(29040..30419)
                     /locus_tag="IYE22_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ9505687.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(30594..31763)
                     /locus_tag="IYE22_000450"
     CDS             complement(30594..31763)
                     /locus_tag="IYE22_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ9505688.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(31738..32889)
                     /locus_tag="IYE22_000451"
     CDS             complement(31738..32889)
                     /locus_tag="IYE22_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ9505689.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(32849..34210)
                     /locus_tag="IYE22_000452"
     CDS             complement(32849..34210)
                     /locus_tag="IYE22_000452"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ9505690.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(34207..35208)
                     /locus_tag="IYE22_000453"
     CDS             complement(34207..35208)
                     /locus_tag="IYE22_000453"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ9505691.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(35224..35919)
                     /gene="dapD"
                     /locus_tag="IYE22_000454"
     CDS             complement(35224..35919)
                     /gene="dapD"
                     /locus_tag="IYE22_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ9505692.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(35925..36641)
                     /locus_tag="IYE22_000455"
     CDS             complement(35925..36641)
                     /locus_tag="IYE22_000455"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ9505693.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(36638..37519)
                     /locus_tag="IYE22_000456"
     CDS             complement(36638..37519)
                     /locus_tag="IYE22_000456"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ9505694.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(37522..38322)
                     /gene="dapF"
                     /locus_tag="IYE22_000457"
     CDS             complement(37522..38322)
                     /gene="dapF"
                     /locus_tag="IYE22_000457"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ9505695.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(38309..39568)
                     /gene="lysA"
                     /locus_tag="IYE22_000458"
     CDS             complement(38309..39568)
                     /gene="lysA"
                     /locus_tag="IYE22_000458"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ9505696.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
BASE COUNT        10973 a         8332 c         7194 g        13257 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga
    26641 agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat
    26701 actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga
    26761 atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta
    26821 ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga
    26881 aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata
    26941 agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga
    27001 agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt
    27061 tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg
    27121 taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat
    27181 tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc
    27241 catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca
    27301 agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca
    27361 ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt
    27421 gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg
    27481 tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg
    27541 acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc
    27601 cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta
    27661 tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact
    27721 aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct
    27781 ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc atggaatata
    27841 caaggaagaa atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa
    27901 agttagactt ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt
    27961 tttacacaac cgttacggtt gttttacgaa attggacgag actttgccag tttaatttct
    28021 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
    28081 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
    28141 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
    28201 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
    28261 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
    28321 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
    28381 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
    28441 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
    28501 gatggatccg atttttggct actgctccaa cttttcctcg ataagaattg tagcgacgtg
    28561 atttacgcgt gtatgccgtt acttttattc ctagcttctt gatcaagcgc tgaactttct
    28621 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
    28681 ccttgtgctt ctcccgaatt ttaagcattt ccgcttcaat ctcttgatct ggatttttac
    28741 ggtccaatcg cctttgccat ccaaataatc atgaacgatc tttaatttaa gttcaaaagt
    28801 atatttaacc ataaaaaata ccgacctcca aaagttagat tttttggtct aacttttggg
    28861 ggtcggtaca caaatcgtgt cacagtccct tttttgaaaa taaacggtgt tcaaaagctt
    28921 gcatctgcgg taataagtcc agccaagtta aaaacataag aagctgtttt tacttggctg
    28981 ttcttattac ttgatgcaag gcagcttttt cacaacctct tttcaaatat ttcgttttct
    29041 tattctacaa atggttcttc tcctaacgct tgttgagcgg ctagattcaa catgctccat
    29101 tgtcggtcaa agcctggctg gaagaagaag tcagcttctg ctagttcttc taaagtcagt
    29161 ttgttttgga tcgctagtgc caatacattt ccttgacctg taacatcata ggtagataat
    29221 actgctcctc ctagcaattg atgggtcatt ggatccgaga ataattggat ggacactttt
    29281 ggatttcctt gttcttctgg tacatatttt ggtcgtaacg tatcttcata ataagacact
    29341 cgtacatcta ctcctgcttt tgctgctgta aagctattca atccgcttgc tgcaaaatga
    29401 tagtcaaaga cacttaatgc agaggaacct acgactccgc cgaacggttt gtttggtact
    29461 tcttcaaaaa gatgttttac tacataacgt gcttctctac gtgcaacagt cgctaaagcg
    29521 atcggcattt ttttgcgtgc tgggatagaa taagcaagtg tagcatctcc tactgcatac
    29581 acatctggaa gattcgtttg caagtattca tttgtttgga tccagccacg ttcatctaag
    29641 tcgacgatcc cttttaacca ttctgtattt gccttcacac cggcagcttg aatgactaga
    29701 tctgctggga tttctttttc acttgttttg attgcctcaa cttttccgtt accagtaaaa
    29761 gcttcgattt ttgcatttgt caaaacttca ataccgtttt catttaggtg ttgttctaaa
    29821 atatctgtca tctcagcatc taaataagtg cctaaaggtc tatcgatcac atcaagcaat
    29881 acgacttctt tcccagcttt tcggcttgct tctgctgctt cgatcccaat gtaacccgca
    29941 cctacgacag cgattttttt aatagccgga tcatttaatt tttctttgat tttggttgcc
    30001 caattgtatc cacgcatcaa gtagacattt tctagatctg ctcctggtac tggcagattg
    30061 tttggtttga caccagaact caaaatcaat ttgtcataag gatattcttt tgtttcgtta
    30121 tctgatgcgc tagtaaccgt tactgttttt ttatcggcat cgatcgtttt tacttcgtga
    30181 ttatttaaaa tatgaacatt ggattgcgga aagttttctg gtctgaaatt acgtacatca
    30241 tttacatctg ttaccttgtc ttctaaatac agctccatac cacatgacat gaaggaaata
    30301 tagtctcctg cttcaaatag cgtgatatct acattttcac cgtatcgtgt caatagttcc
    30361 aagatcgatt gatgccctcc atgagatgca cctacaatga caactttgat tttttccatt
    30421 gatgaaacct ccttgaatat tcttttaaaa tcattctaaa caatttaaac ttaacattac
    30481 attagttatg aatcaaaaga aacgcgttca tttgaactaa atttaaaaaa caatcgtctc
    30541 cttgcagaat ataccgcaaa attaacgatt gtttttttga taaaatctat gaatcactgt
    30601 tctgttaaaa agtctttgat ccgtttgcag gcctcatgta atttttccat gctgctagca
    30661 taagaaatcc ggatataacc ttctccttct gggccaaatg caattcctgg gatacacgca
    30721 acttttgcct gttccgctaa ttctacacaa aactgccaag agtcagtttg aatggcagct
    30781 gggagtttgc aaaacaagta aaatgcaccg tttggctggg tcacttcaaa tcctaaaggc
    30841 gccaattgct ggaccagata gtctcttcgt gtttgatatt gctctttcat attctcgcta
    30901 tctttcaccc catttattag cgcttcagcc gcagcttttt gcgacatcgt tgtagcggca
    30961 gtcaccagat attgatggac tttaattagt tcgtcaataa gggctttttg agcaaaaata
    31021 aagcccactc gccaaccagt catcgcatgt gacttggata acccgcttat cacgattgtt
    31081 tgttctggaa gataagtagc tatggacata tgcgttccac tataagtcaa ttcagcatat
    31141 acttcatcac ttataacaaa taccggatat ttttttaaaa cctcagcgat tgctttcaat
    31201 tgttttgcag acaatgtcgt cccagtagga ttgttaggat agtttaaaat gactgctttg
    31261 acagctgatc catattgttc aaatgctcgt tcaaattgtt ccggctgaca gacaaaccct
    31321 gtgtcagaag tatctatcgg gatcaattca gcaccagcta gttccactaa tggctgatat
    31381 cctgaataag caggcgctgg aataagaacc ttgtctcctt cttccaaaac agataacaac
    31441 gcagaggcaa tcgcttccgt tgcaccaact gtcgtcaaaa cttccgtttg cggatcataa
    31501 gttaatccat aacgctgttg ctgaaatata caagccgctt cccgcagttc ctctaatcct
    31561 ctcattccgg tatagtgaga aaagtcttct tcaatcgctg ctattcctgc ttgcttgaca
    31621 tgttcaggag ttggaaaatc cggttctccc agggtgagtt tgatcacatc tggtatcgac
    31681 gatatttgct gatcgaattg tcgtattttt gatacttcta ttctagaaag cctaggacta
    31741 aatcgattgg ataaattcat cattatttac tcgctcctct tttgaactgc tatcgatttt
    31801 tacaagaaga ttttctgtaa aaagaatcgt tttgtaccat tctacaccat tttctaaaac
    31861 atccatatca aaatcgaacg tcggatcatg taaagcaggg gtatcaccga cacctaaaaa
    31921 gaaaaataca catggatatt gttgcgtata tatgccaaaa tcttcggctt gaagaactgg
    31981 agcatctaat tcatgtagtt tgtttgaatc taacagtgct gcatataagc ctctatggtt
    32041 gattacagct ggatagccgt cattatagcg aaaagaaata gtagctgtag agtgaaactg
    32101 ttctgctaaa tggtctaatc ccctcttcaa ataattttgc atatctgaac tgtaagaacg
    32161 gatacttccg gatacaataa cttctccagc taaaacattt cggatcgttc caccttggat
    32221 ttggccaaat ttcattagat gattccactc tgccggtaat tgccggtcaa aatcataaac
    32281 attcttcaaa aatcgtacag ctgcctccag actatcgatt cctttttcac tagaagcaat
    32341 atgtgcagac tggcctttaa tgataatgtc tgtttcactg ctttgtgcca tcaaaggtcc
    32401 tggtcgagag aaaacttctc ccttagctag tccaggccat agatgaaagc caaatattgc
    32461 ttgtacttca tatttatcca acaatcctga ctgaataacg ctttgtgcgc caccggaaat
    32521 ttcttctgat ggctgataga tcagtactac attataaata agatcctcaa tgtgtttagt
    32581 tataaagtca gtcaacccta aaagcatcgc catatgtccg tcatgaccac atgcatgcat
    32641 ataaccttca tgcttagaag caaaagagag attcgtttct tctttgactg gcagtgcatc
    32701 gatatctgtt cgaaaagcaa tcgtttcttc ttgctgatta tcaaaataaa ggagtagtcc
    32761 tgtttcgccg atttcatgga taacggcctc gctgtttttt acttgttcat ataaatattt
    32821 ttttgtcttg aattcatgaa acccgatttc aggaatttga tggagtgcca ttcgatattc
    32881 tttcagcatc cgtattcacc atcccttcat agataccctt cactactgtt tcatattgat
    32941 tattcggtac accgatgatc agattcaact cctgtgctcc ttgtgataag atcgatgtgc
    33001 gaatttctaa tttgttcaat atcgataaca ctttcccaga taatccaatc aattctttat
    33061 gcggaccccc aacgacagag atcaaagcta agtcttcgat cacgacgatt tcttccacgc
    33121 caagtgtttc ttttaattct ttcgtgatga gaaataactt atcggcaatc gcttctgctg
    33181 agacgatcac tccgatattg tcgatccctg aaggaatatg ttcgattgat atctcatgtt
    33241 tagcaaaaat gctcattgct ttccagataa acccgatttc atcggacata tgacgtttga
    33301 ataaagtgat ggaaagaaaa tccttgcgtc cagctattcc tgtcagtcca ttttcctttt
    33361 ctatttcgtg attcgatatt tttgtccctt gagcgaaagg atcattggta ttttttattt
    33421 ggatggggat atcagcctct tgaactggga aaacagctgc ttcatgaaga acgttagccc
    33481 ccatataagc catctctctc agttctctaa aagagatttc ctcgatgatt ttaggatgat
    33541 cgatgattcg cggatcagcc atcatgattc ctgaaacatc cgtccaattc tcataacttt
    33601 ccgcttgcaa catcttcgcc agtatcgcgc cggtaatgtc gcttccccca cggcccaata
    33661 atttttcttt tcctgcaggg ttcgccccgt aaaaccctgg aacaaccatt ttcggatatc
    33721 ttttaaatgc ttttgtgatt tcctgtgctg ttttttcctg atcgattttc ccatcatagt
    33781 caaagaaaat caggtctttt gcgtcaataa actggtaacc aagatactca gccatcagtt
    33841 gagcagtcag atattcacct ctacttacta aataatcttc agttatcttt tcgtcttcca
    33901 gttgtttctg tatgcgtgct agttcatctt caataggtaa ctgcagggca agaacatctc
    33961 ttacttgaat gaaacgatca caaatccttt tccaaagggg tgtccaatca acatgatgct
    34021 gaaggtaagc ataaatcaaa taaagtaagt ctgttacttt gtcatcatct gacgtacgtt
    34081 tgcctaaagc agaaacaacg atcacttggc gattgggatc tgaaagaaca attgtacgga
    34141 cttttttgaa ttgctcttca tgagacatcg atgttccgcc gaatttcgct actttcagcg
    34201 tattactcat cttgccacct ccgctatttt ctgcattaaa gctgcagcaa tctgtacggc
    34261 attcgcagcg gctcctttgc gtatgttatc ggcagtcgta tagaacaaca atccgttctc
    34321 taaagaagtg tcttttctga tcctcccaac ataaaccata tctgttcctc tagcaagaat
    34381 gctcgtcgga tatttatgtg ctgctagatc atcttctaat tgaatccctg gaaactggct
    34441 gagacactct ctaatttctt ccgtcgtaaa ctctttttgc agttgaactg ccacagagac
    34501 tccatgtcca ttttcgattg gcacacgtac gcaggttgct gatactggca aagcaggctg
    34561 atgcagaatc ttacgggtct catttatcat tttctgttct tcttttgtat acccattttc
    34621 caaaggctga tcgatttccg gaatcaccgt ttggctaata ttataaggat acaaaactgg
    34681 ttcctcgtca ttttgcgtac gatgaagatc ttcaatgcct ttttgcccgg aaccagaaac
    34741 tgcttgatag gtcgaataat tgactcgctg gataccaaag gcttcttgta aagctttaag
    34801 cggaagtacc gcttgaatgg ttgaacaatt tggatttgca atcagccgat tcaatgtata
    34861 gtcttccaga ttgacctctg gaacgattaa tggaatatcc gaatcttccc gccatgctga
    34921 ggaattatct atgacaaata gtccctgact tcgtgcgatt ggcgcaaact ctttggatac
    34981 agaagcacct gcagaaaata gcgctaaatc atatccttca aatgaagttt ctgtcaattc
    35041 ctgtacagtg tgtttctttt tcttgaatac acaaacttta ccggctgaac gttttgaagc
    35101 taataatttc aatcgtccta ctggaaaatc atattcttct aacactttga gcatcgtttg
    35161 gccgactaat ccggttgccc ccacaataca ggtatctatt tcgcgcattt ttctccatct
    35221 cctctataat tcgcgtaacg cagcaatcaa tgctgtattt tcttttgtct gatcatcact
    35281 tatttttaat acacgtgcag gtattccacc gacaacagtt tctgctgcca catcttctaa
    35341 aaccactgct cctgctgcca cgactgcatc ttttccgata tggacacctt caacaatcac
    35401 tgcattcgca cctaccagta cgccgtcttc tacgataacc ggtttggccg atgctggttc
    35461 gatcacacct gctaagacag ctcctgcacc aatatggcag tttttgccca ctgtcgctcg
    35521 tccacctaag acagcaccca tatcaatcat cgtgttttca ccgattactg caccaatatt
    35581 gataatcgct cccatcatga tcacggcatt gttgccgatt gacacttgat ctctgataat
    35641 agcacctggt tcgatacgag cattaatttt tttaatatct agcaaaggaa ctgcgctgta
    35701 tcttgcagca gtttcgattt cgatttcaat aattttttct gattcagctt ctaaaaccgg
    35761 agcaatcgtc tcatagtcac caaaaacaac gaagctgctt ccttcaccaa aaactttact
    35821 ttctttaaat tcgattggct cggttacatt cagatagact ttcacaggcg tctttttaga
    35881 tgccgtttga atgtagcgga tgatttcttg agcttccacg ttttttacac ccctttgtcc
    35941 attaataaat cttctaattt gtaataacct ggtgactgat ctttcaacca gtccgctgct
    36001 tctactgcgc ctgcagcaaa gattcgatta gaaaaagatt catgtttgat cgtaaatatt
    36061 tcatcagtag cagcaaacaa tacatcatgt tcaccaggta aagaacctgc acgaatactg
    36121 tgtacaccaa tttggttttc ttctcttttt ttcccttgcc agttgtatac gggctgcagc
    36181 ttttgcactt cttggattgt ttgaagcagc atatttgcag taccagatgg tgcatctttt
    36241 ttacgactat gatgtttttc aatcagttcg atctgccagc cttgcaacat tgcagctgct
    36301 tgcttgatta gttgattcat caccatgatg ccgaacgaaa aattagcact atacatcaca
    36361 ggtacatgtt tggcatgttc ttttatttct tggaactgtg attgtgtata acctgtcgtg
    36421 ccaatgacta acggaagttg atatgataag ctatacgaaa ggattttctc taaattatct
    36481 ggatgagaaa aatcaataag cagttctggc agttccgtaa aagcaagcgt agttgcttct
    36541 ttaggtgccg tccgtaaaag aaggaccgta tctcctcttt cttgtgcaac ttcttcgact
    36601 ctatttccca tctgtccact gccaatcagt ccgattttca tttttcaacc gtcctttctt
    36661 ttaacaaaag ctgagctaat tgtttctttt tctcttttga aagagaaact aaaggcaggc
    36721 gtagaggtcc ggaaggtaag tctaataatg acatggctgc tttgacagga ataggattgg
    36781 tctcactgaa caaggcatgg atcagttcaa gtgcatttaa ttgcttttct tttgcttgct
    36841 gccatcttcc gtcctgcgta tcttttacta attcatggac tattttaggt tggatattcg
    36901 cccatacact tattacaccg cttcctccta cactcattac tggtaggatc aaatcatcat
    36961 ttccactgta aaggaagaat tcttctccaa tcaaccgagc ggcatccatc acataagcca
    37021 tatctcctga ggcttctttc aaaccaatga tattaggatg ttctgctaaa tttactaata
    37081 catggattgg aatcgacatg cctgttctag aaggaacatt atacaaaata agcggaatcg
    37141 gtgaggcttc tgcaattgct gtaaaatgag cagctagtcc tgcatcactt gttttgttgt
    37201 agtaaggggt gatgaccaaa agtgcgtctg cccccaaaga agcaaaatgc ttcgcttttt
    37261 caatcgtttc tttcgtatta ttcgttccag ctcctgcaat caccggcaca cggccagcca
    37321 ctttctttac tgtcagttga aggatctgct cctcctcaga ttgtgtgagc gtagaagatt
    37381 ctcctgttgt acctaaaact aataatccat ctgtttgttc atctaaatgg aattggatca
    37441 atttttccaa accagcataa tctactgatc cctcttcatt catcggcgtg atcagcgcca
    37501 cgatcgaacc ttttatcatc ttcattcctc caatattctt gtccattcat gaactgctgg
    37561 gccagccatt tgtatcgcat cctcaccaga gatcatcaag tctccttgtt ccaagtgaac
    37621 aactacctga tctgctgaaa ttttcccagt ttccttagca accacataag aggcacagca
    37681 acctgtaccg caagcaagtg tccaacctac tcctctttca taggtacgga cgatcaattc
    37741 ttgttcattg actacttcta caaagttgac attggttttt tctttgaaca aaggatgatg
    37801 acagatcagt tcacctcttt ttctccagac ttcctcacgc gcattttcaa caaaaacaac
    37861 ggtatgaatc gttcctaaaa ataaactagt gatcgttact ttttcaccat tgatctctat
    37921 ttcctggttt tttattggat aagtttgatt agctttgatt ttttccggag aatcatctgg
    37981 cactcccata tctacgctgc aataaaaagg tgagggatcc aacaccgtaa tttttcttgt
    38041 gcctgctaat gtctctacgt tgaattcctg ttcattcatc atgttctgat catttacgta
    38101 tcgagcaaaa cagcgaatgc catttccgca catcggcgcg cggctgccat ctttgttgta
    38161 atagatcatt tccaacggat cgatttttac ggcgatcaat ccgtctatat catatttttc
    38221 ttttgtgcat aaagaagcgg ctaattgact ataatctgtg tcttctgcgt aatctaataa
    38281 gataaaatca tttccgcagc cattatattt atgaaggatc ataggaaact tcccccctta
    38341 gtagatcaga aaatgcttgt ctgcgaacaa tcagtcttga ttggccatta ttgacaaaaa
    38401 caactgctgg tcgtgtcgct cgattgtagt tgctgctcat cgagtatcca taagctcccg
    38461 tggaataaac agctaaaaga tcacccgatt ctgctttcgg caaataagct tctttaatta
    38521 ccacatcgcc tgattcacac atttttcctg caatcgtcac tttctctgtt ttttcttccg
    38581 acagttttgc tgctaaatca caatcgtatt ctgcctgata cagagctgga cggatattat
    38641 ctgtcatccc gccatctaca aagtaatatt ttttatgtgg tgtctttttg atgaaaccaa
    38701 tcgtgtatag tgttgtacca gcttctccaa caatacttct acccggttcg atcagtaatt
    38761 gacgaatggt cagttttcgt ttttccaaag cttcttccgt atattcaatc agttttttta
    38821 gtgcctcttc gactgggatc ggctgatcat tttgtgtata acgaatccca aaccctccgc
    38881 caagattcaa ggaaagaggt tggtcaaaat cggttaagta attcgtcaat ttgtcgattt
    38941 cagctaacca agcagtaaca tcaaaaattt gtgacccgat atgagcatga aatccttcta
    39001 actctagata gctgctttct tttatcaact gaagggcttg ttggcactct ttactttcat
    39061 agctcatgcc gaattttgaa tcaatatgcg cagtgacgat atattcatga gtatgtgctt
    39121 ctactccgac attcaaccga agcatgatac agattttttt taaatagcgt tggcttaatt
    39181 ctgctaataa ttcaacttcc atcaaattat cggcaacaat atgcaacaaa tcattttcaa
    39241 gtgcatagat cagttcctct ggcgtcttgt tgttaccatg gaagtagata gtatctctag
    39301 gaaaatcagc ttgcaaagcg gtatagattt ccccaccact gacaacatct agcccgaatc
    39361 catattcacg aactaaatgc agcatctcaa tcgtttggaa tgctttagag gcgtaaagca
    39421 cttttgtatc aaaacgtttc gatacaaagc ctcgcttgaa tgacagcatc gtttgtctca
    39481 tcttttcttc atcatacaca tacagaggtg tcttatactt ttctgccaat gtgcttgctg
    39541 tgactccgcc aattatcaat tctgtcattt tttcattcct cttttcactt tttttccgtt
    39601 tttattggaa tttcatttga taaagatttg aattttattg gaataatatt aaacaaaatt
    39661 ttctgaattg tctagtatat ttcgaaatat ttttttaaaa ataagaataa aaatggttat
    39721 aaaaacaaaa tacccgaact attagaagct atttat
//
LOCUS       DACIRY010000011        38664 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.011, whole genome shotgun sequence.
ACCESSION   DACIRY010000011 DACIRY010000000
VERSION     DACIRY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.011"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(365..991)
                     /locus_tag="IYE22_000459"
     CDS             complement(365..991)
                     /locus_tag="IYE22_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505697.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1286..1621)
                     /locus_tag="IYE22_000460"
     CDS             complement(1286..1621)
                     /locus_tag="IYE22_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ9505698.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1608..1892)
                     /locus_tag="IYE22_000461"
     CDS             complement(1608..1892)
                     /locus_tag="IYE22_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ9505699.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1948..3471)
                     /locus_tag="IYE22_000462"
     CDS             complement(1948..3471)
                     /locus_tag="IYE22_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ9505700.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3464..4639)
                     /locus_tag="IYE22_000463"
     CDS             complement(3464..4639)
                     /locus_tag="IYE22_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ9505701.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4643..4828)
                     /locus_tag="IYE22_000464"
     CDS             complement(4643..4828)
                     /locus_tag="IYE22_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505702.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4794..6488)
                     /locus_tag="IYE22_000465"
     CDS             complement(4794..6488)
                     /locus_tag="IYE22_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ9505703.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6485..6958)
                     /locus_tag="IYE22_000466"
     CDS             complement(6485..6958)
                     /locus_tag="IYE22_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ9505704.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7027..7182)
                     /locus_tag="IYE22_000467"
     CDS             complement(7027..7182)
                     /locus_tag="IYE22_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ9505705.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7316..7696)
                     /locus_tag="IYE22_000468"
     CDS             complement(7316..7696)
                     /locus_tag="IYE22_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ9505706.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7700..7915)
                     /locus_tag="IYE22_000469"
     CDS             complement(7700..7915)
                     /locus_tag="IYE22_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505707.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7918..8325)
                     /locus_tag="IYE22_000470"
     CDS             complement(7918..8325)
                     /locus_tag="IYE22_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ9505708.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8591..10066)
                     /locus_tag="IYE22_000471"
     CDS             complement(8591..10066)
                     /locus_tag="IYE22_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ9505709.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10056..10904)
                     /locus_tag="IYE22_000472"
     CDS             complement(10056..10904)
                     /locus_tag="IYE22_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ9505710.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10941..11303)
                     /locus_tag="IYE22_000473"
     CDS             complement(10941..11303)
                     /locus_tag="IYE22_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505711.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11603..12227)
                     /locus_tag="IYE22_000474"
                     /pseudo
     CDS             complement(11603..12227)
                     /locus_tag="IYE22_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12224..12535)
                     /locus_tag="IYE22_000475"
     CDS             complement(12224..12535)
                     /locus_tag="IYE22_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505712.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12579..12872)
                     /locus_tag="IYE22_000476"
     CDS             complement(12579..12872)
                     /locus_tag="IYE22_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505713.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13066..13713
                     /locus_tag="IYE22_000477"
     CDS             13066..13713
                     /locus_tag="IYE22_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9505714.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13774..14919
                     /locus_tag="IYE22_000478"
     CDS             13774..14919
                     /locus_tag="IYE22_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9505715.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15000..15305)
                     /locus_tag="IYE22_000479"
     CDS             complement(15000..15305)
                     /locus_tag="IYE22_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ9505716.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15330..15761)
                     /gene="ruvX"
                     /locus_tag="IYE22_000480"
     CDS             complement(15330..15761)
                     /gene="ruvX"
                     /locus_tag="IYE22_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ9505717.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15758..16024)
                     /locus_tag="IYE22_000481"
     CDS             complement(15758..16024)
                     /locus_tag="IYE22_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ9505718.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16279..17004)
                     /locus_tag="IYE22_000482"
     CDS             complement(16279..17004)
                     /locus_tag="IYE22_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ9505719.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17365..17435)
                     /locus_tag="IYE22_000483"
     tRNA            complement(17365..17435)
                     /locus_tag="IYE22_000483"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17401..17403),aa:Gly,seq:ccc)
     gene            17552..18304
                     /locus_tag="IYE22_000484"
     CDS             17552..18304
                     /locus_tag="IYE22_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ9505720.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18320..18799
                     /locus_tag="IYE22_000485"
     CDS             18320..18799
                     /locus_tag="IYE22_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ9505721.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19050..20630)
                     /locus_tag="IYE22_000486"
     CDS             complement(19050..20630)
                     /locus_tag="IYE22_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ9505722.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20623..21366)
                     /locus_tag="IYE22_000487"
     CDS             complement(20623..21366)
                     /locus_tag="IYE22_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505723.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21542..22318)
                     /locus_tag="IYE22_000488"
     CDS             complement(21542..22318)
                     /locus_tag="IYE22_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9505724.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22566..23402)
                     /locus_tag="IYE22_000489"
     CDS             complement(22566..23402)
                     /locus_tag="IYE22_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ9505725.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23413..24342)
                     /locus_tag="IYE22_000490"
     CDS             complement(23413..24342)
                     /locus_tag="IYE22_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ9505726.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24534..24887
                     /locus_tag="IYE22_000491"
     CDS             24534..24887
                     /locus_tag="IYE22_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ9505727.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24961..25617)
                     /locus_tag="IYE22_000492"
     CDS             complement(24961..25617)
                     /locus_tag="IYE22_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ9505728.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25843..27777
                     /locus_tag="IYE22_000493"
     CDS             25843..27777
                     /locus_tag="IYE22_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9505729.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27863..29086)
                     /locus_tag="IYE22_000494"
     CDS             complement(27863..29086)
                     /locus_tag="IYE22_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ9505730.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(29080..30978)
                     /locus_tag="IYE22_000495"
     CDS             complement(29080..30978)
                     /locus_tag="IYE22_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ9505731.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30972..32090)
                     /locus_tag="IYE22_000496"
     CDS             complement(30972..32090)
                     /locus_tag="IYE22_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ9505732.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32173..33006)
                     /locus_tag="IYE22_000497"
     CDS             complement(32173..33006)
                     /locus_tag="IYE22_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9505733.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32999..33915)
                     /locus_tag="IYE22_000498"
                     /pseudo
     CDS             complement(32999..33915)
                     /locus_tag="IYE22_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(34142..35266)
                     /locus_tag="IYE22_000499"
     CDS             complement(34142..35266)
                     /locus_tag="IYE22_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ9505734.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35449..36357
                     /locus_tag="IYE22_000500"
     CDS             35449..36357
                     /locus_tag="IYE22_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9505735.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            36498..38111
                     /locus_tag="IYE22_000501"
     CDS             36498..38111
                     /locus_tag="IYE22_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ9505736.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        11701 a         7709 c         6342 g        12912 t
ORIGIN      
        1 gagactgtga agtcaacgtt ttgacctgat tattagagaa acaaatggca aaatagttct
       61 tcatatttcg ccgtttttcg agcgaatgcc gaggggcagc tgattgaaca tcgtttattt
      121 ggaaaaaggg attgtgacaa gacgtttgtc acaatccctt tttttatatc ttattaattc
      181 attcttttgt tctattcttc ttcttccgcc atgaatgaga aacccatatc aaagaactat
      241 aaaggttatc aaaacagcgt ttatgactaa atgaattaga gttatctatc actatttttc
      301 aagcgattta aaccaaatgg catacaatat ggcatacaaa aacttcaata agccgtgggg
      361 gtttttaaat ttcttcttgt tctttaaaat caatgagtgg agctacataa tttgaatcca
      421 ctttttcaaa cgtcactttt tgcatcgacg aatgttttct ttcccaagat aacgtaaata
      481 gatttgactc tggacttata aagtctactt caccttctag tattactttc ttatgcaaat
      541 attcctctaa taaatataga cttgctaaag catacaacac attttccaaa tttgcttcct
      601 tataatttga agaacgacca tgttttactc tgttatatat tttccaccaa tctggtgtat
      661 cttcaggcca cccatctcca aaaggtttta gtgaagtcaa tgttaaagaa ttcataaccc
      721 taacaccatt atccaaacac attaattcgc tagatttaat aacagcttta aaatcagtta
      781 tgcttttatt gctactgcta tcaaatccgc aaagttcttt gaaaataatc tcacactccg
      841 aaccaatcgc tagtagtagt ttttgatatt caatagagaa tgttttgaaa ttattctcat
      901 cgagcatcac aaaatctgtg gtattaataa actcattttc taaatgtaga taatacttcc
      961 aataaaaatt tgtaaactct gctttgtcca ttaaatcacc tcttttgaat tataacaaaa
     1021 aaaacacgtc cacaacagac gtgttctttg atttctacac aatcagcttg ccaaaaccat
     1081 ccaatcagtc ctagcatgcc tgtattaaaa ttgaacgttc atcatctatc atctttctta
     1141 gtccagcatg tttgcaacca cacctacaat tttcagtcca acaatagatt tcgactagct
     1201 aagtcttgca aaattcaggc agctaaccct tcatttacta ccttaattat actaaatacc
     1261 cacaacgttt acaagtgtaa tcattctaag aaatctgttt agctatgatc gttgtataat
     1321 ctttaaacgc cgttccttca ttgattgaaa taattttata actcttgttt ttccaagcta
     1381 ctttcatatc gtttgttagt tcataatctt gatcgtatcg aataataaac gtcaacgttc
     1441 cttcaagaat ggtacctaca ctagcaagca cgtcttttag catttgtgaa cgcacattag
     1501 cccaacattc aaatacagtt tcttcactat ccaccatctg cccatcttca tcttttaccc
     1561 gttttgtctt tttaaacttg actctctcag tcaattcatt tacattctta atgattggca
     1621 taataagcac ctctcatttg ttgtactaat gcttgtaccg ttactggtat ccgttcgctg
     1681 ctggcttctt gacggttcaa ataccaatgt tgagtaagta atgacaccgc caaatcaaat
     1741 tgctgataat tatccatatc tttatccgtt gcgctactat caatagcact ttttatatat
     1801 tcgcctgctg tatcaatcaa ttgttgaata aagtcatcat caatagtgtg atcgatccgc
     1861 atactttttt ttatgctatc caaaattacc attttatctc tcctaatgaa aaaaaggcag
     1921 tgggataaac cgccgcccaa tatttttcta tacttttggt gttacaactg gcgtaaactc
     1981 aatatacact gaagcatttt gatcaatttt cttataatcg ttgcgaacga tcactgcaag
     2041 tccttgtgag tagtaatcga atttttccca ttgagtcgtt acttgatttc tacgagcaac
     2101 aaagatagat tgtgcaatat cccccataat catagggaat gttccagctt taggattggc
     2161 aaacaatgta tctgcaatca atactactgg cataccgaat aatgatttac cactaggtgc
     2221 tgtcacatcg ggttgtaaaa tgtaacgtcc atctgaatct ttcaatgtat ctaggtggta
     2281 atatccgctt tggtttagaa ttaccatttt atttaatgct gggtctaatg ccacattgta
     2341 tagttgtttc aaatcgtcca acgtagcagc cgttttctta gtgaatgttt ttaataaatc
     2401 catgatatgc ttattgtcag tgttttctac caatttcgct aactgatctt tgacctcttg
     2461 tacaatattc actgctgaat cttccacaac ctcatttgat aaggcaatct taccagcacg
     2521 agtttctact ttataatcaa ctgaagtaaa catttcagcg tcaatatcac caatttcagc
     2581 aagttcagct tttgttgcta acactgcttg ttggttagtt gctactggat atttaccttg
     2641 tccatttgat actgttttta ctgttgcata ttgagctaag ttataatttg aacgtttcaa
     2701 atcaaagact tcaccgatta cttcttcggg aacaactgcc gctgcattta cagtagtgac
     2761 tccatcacgc acttcgcctt gtgaacggat atattcttca tacccacgca attctgtttt
     2821 ttcgttatcg attaaagttt tttccatgtt attactctcc ttttgtgctt tagaagtgga
     2881 ttctaagcta ttgtttgttt gttttgcttg gttgttactc ataaacgatt cgtaagaacg
     2941 cttatttact tggacatttg atgaatcata agcgggaacg gtcacgacac taatttcatt
     3001 aagtgctttg attttatcta ttgatcgttc aattgtgcca tcttcttttt tgtcgaatga
     3061 atctaatcca agcacgaacc caaaggacat agaatcaaca accccttttg agatattctc
     3121 atacacatca tttgaatagg tcgtgtcggt tagctctgct tcaaaatgta atcctgtatc
     3181 atcgacattc aatttcaacg tacctgcttt gacactagct agtggctttg aataatcgtg
     3241 tccatataat aagaacacat ttgataaatc cacctctttt aatgcttcgg gtgtaatcac
     3301 ttctacaaag ccgcctaaat cttttgacgg ttgcccgaat ttcaacgcat agcctgaaat
     3361 agttttgccg tctgttggtt gtttttcatt ttctttctct actttgggag aatcggctgt
     3421 tagctcagct tcttctgtca gccgtttttc cttttcttct tcattattca actggtgtaa
     3481 ctccttcctg ttcttttaat tgatatttct ccaacgtatc taaatacgta aagttcaaac
     3541 ttgctagtaa acgatcgcca ccatccatgg ggggtagccc cattttcgac cgcccctcat
     3601 taatcgtgag aagtgaccct ttgacctgat ccaatacatt ttttactttt gtttcaggat
     3661 cagtctctag taactgatcg gaattaaagc ggatattggt ttcaagtttc gtatctaatt
     3721 cactcacaaa cacattaaag taatgaatca gcgtactttg gatatactgg agattacttt
     3781 gaacggtgct agaatgttca ttttcaacgc ctaaacgctc aatcggtaca ccaaacgcct
     3841 tggcaatttg ttttgtgttc caatcattgg aattgataag cttcaaaaca tcggtgttta
     3901 tttctaatgt tttgtagtcc attgtttcat caagaatgat cgttctaaga gcattatctc
     3961 cactacttga accgttggct tcttcaaact tttcacgaat tgcgttcttt gctgatccgt
     4021 ctaaatctga cttatgaacc ttcaaaatac ctgacccact gacaccacga gtaaagaaat
     4081 tgtgcaatgt gcggttgcca gcttgttgaa ttttcagttc atcacgtaac gcataaagtg
     4141 gtggtaatcc tgttaaacca tcttgggtga aatatttgaa gtgcaaaata tcgctagact
     4201 tcacacgtct ttttttatca ccaatctgat aagacaacgt accattatct aattgagtaa
     4261 ccgttacact tgagttaggt aataagtgaa tttcttctac cttttcaccg ttgcgtttaa
     4321 tctctgcaaa gctattaccg tttagcaaca tattgaccgc caaagcaaac tttaaatgcc
     4381 aaccatccat ttctgaatta ggtttctcgt ttagtaactt caccagttca ttatcagctt
     4441 gtggcatatt gtttttgacc aattgaatcg gacttgaagc aatatcacta gcaataattc
     4501 gaacggctgc aaacacatca ctatttctta atgcacgaac gcttgtaaag ctggtggtgt
     4561 aatcatccga ttggattgat accacatgtt gtaggaaagg atctcccgtt gtattttgtc
     4621 tcgctttaaa aaatgccatc ttttaacctc ccttctgttc tgtatttagc atgaccgcta
     4681 ccccaataag cactatgccc agcgctaaca agccatagta gacatttgtt agtaaggtga
     4741 tagcaaccaa cacacacacc aatccaagaa gtaaaagaac ggtatgaatg taattagaaa
     4801 gaaaattctt cacttgtata aaattcattg tctgctttcg ctccttccgt tgtggtgaag
     4861 taatccattg caaacacata ggcattgatt aatgcagcta ttggatcaat tttgttactg
     4921 ttctttgatt tattaatctg aatgccgttg ttatccgttt ttaaaatagc gttgttcact
     4981 gcatgggtga gaatcgtatt tttattgtga ataacgttgc cttcataaac ttgttctcta
     5041 aaagttcggg tcggaacgtt tagagtaatc gttccttgtc ttacttctaa cattgggtag
     5101 ttggcttttt cagctttact gataagcgaa ttagcgttat acgggtcata acaaatcccc
     5161 atacattcca aatcattttc ttgaatcaaa tcttgaataa actgaaatac ttggtcatag
     5221 tccacgattc cactttctaa ttgagtaatg gaacactcac cagctttttc tagttctcga
     5281 taaggcaaac cgtcgcgctt ttctttgtct tgaagcccat atttggtggc tacaaagcta
     5341 tgtgaatcac aataaagttc gccgttatct agcggtacga tccatgaaac gctagttaaa
     5401 tcatctgttt tagataaatc cactccgata taaacgggct tgcctgtaat atcttgtggt
     5461 tctacttcgg ttgcctgcca gtcttcagca atcatatagc tgtcctcact cgcttgtctc
     5521 cataaattga agtttttcac caatacagaa ttaaggttat tttgtttcaa agcaaggttc
     5581 acatcatctt gaatggttgg aatcattact tttttgattt cttcactttc aaaaatcgga
     5641 ttggctttga tccatttctc ttgatcgtga atttcttcgg gatcgtccaa ttcccatatc
     5701 gctataaagt acctgtcggc ttgttcttcg ccttttagca cacgatctaa caataagtat
     5761 tcctcgtaca taggaacgtt taaatcaagc ccagcggtac taataacaca caataagccg
     5821 ttcttttgtt gggtcattcc tgacttaatg acgttgtacg ttttacgagt ttttgcttcg
     5881 tgccactcgt caagaattgc ggttgttggt gcaaacccat ctagcgtgct ggtttcacta
     5941 gccagtgcca tagcgaacga attagagggc aagtgctgaa tcttagagtt cataattttc
     6001 agttgtggtc ttaaaaactt actagacttg accacgtttc taagtgaatt agacagcata
     6061 tcatagccca atttagcttg tttcaaagcg ttacttacaa acaatacttg ccttgcttct
     6121 gctggttctt gttccataat caacgcattg gcagccatgc cacttgcgat atacgttttc
     6181 ccattcttac ggctcatact ggtaaacgct cgattgaatc gcctgtaatt cccttcttta
     6241 gtacgccagc cataaagact gcctagaatg aatttttgaa atcctaacat cttgattgct
     6301 ttaccgtccg tagatggtag caactcaatg aatttaatcg ctttggtggc ttgttcttca
     6361 tcgaacacat aagggaagct atctgactta gaacgctcca aatcgcttat atgacgctca
     6421 cacgcttgga taattttctt gggtgcaatc agcttacctg ataggacttt ctcaatgtaa
     6481 tcattcatga attcatcaac tctttgaacg ggtcttcggg ttcatcattt ttaaccttgt
     6541 tcaaagctag ttttgatcgt gattctaagg tcatacctag tgaggtggct tgttttttca
     6601 aatcactcat agcttgtgat tgaacccgaa ccgctggatt ggctttcttt actccgtttt
     6661 cgcatgtgat aaacgtacct gattttctta tctcggcttc ggctgttttc attcgagcaa
     6721 aggctaaaca ataggctacc aatgaaccat aatctgcttc actcaatgga taatcttttt
     6781 ttaatgttgg tacgatacga ttccattcac ttctagcgct cgctgccatc cagtcagggg
     6841 gcgtgatatt tattaatggt tgctgcttga acaattcttc tctagcgtcc acacggattg
     6901 ctttttcttc acttgatata ttgccttttg tatcgtgtaa tattttaggg tgtcccatgt
     6961 tgtcctcctt tcagacaaaa gaaaaaggaa cgtaagcaat taagctcaca ttccttcgtt
     7021 tttcgatcag aatttttctt tatctaattt atctatataa atgatttttc cgttctgcat
     7081 ttgaatggtc acttgaccaa aatgaggtaa ctcaatggtg cgctgtttcc ctgttttatc
     7141 agtaataaca attgtatttt tcaataaatc tcctactttc attatatact ttttcacttg
     7201 tgattacaag tgtaatcgtt atttgtaatt acccttatgc cttttcacaa cggttatttg
     7261 taccaaaatg agtaggcatc gattgtcagt aagtttcaca ctatgccccc ttaactcaaa
     7321 gggtactctt ttctcgtctt tgtctttcat tggctgtctt ggaattatgg tgtgcatggc
     7381 ataaagaacg aaaattagtt tcatctaacc gtctattcca atcatctttt agttcaattt
     7441 tgtgatctac aacatcagct ttccttatca agccttcttc taggcaatcc tcacatactg
     7501 gatgatttaa ccgatacaag tatgaagcct ttcgccatgt tctactttga tagaattgaa
     7561 aatatttacc ttcgctttgc tttctttctt cacgcttaat tgccgtttgc tttgctttat
     7621 gcttcccaca atacttctgc ctataatcaa tcaatgtttt acatccagca tgattacatt
     7681 gttttttaac tgccatagat taccaaccaa agcgatctcg ttcgtcagaa tcatcttgtt
     7741 cccattttga tttgtattca atataaggcg aaactatctt caagcacgtt ccgtctttaa
     7801 aattaacttt agtgatttta tcttcctcaa aactaataaa gtctacatct tcttgagtgt
     7861 ttgaatactc aatgtgtttc tcaccatcga agtatctaat actttccata tgcataatta
     7921 ttctacctcc aataaattag ttatcaattc atcatctgtt agtagcaaag ccaagaactt
     7981 atcatggatg tttaaaaaca aattatcctc attcgatgta agctctcgtt gtctcgctcg
     8041 atcatatagg ttaagcagac aattaaattc atccattagc tcacctgtat catcatagtt
     8101 gccactatct accaatgatc ccactactag cgattgacac cacgctactg actcctgcag
     8161 ccaatcaggt tcttcttcca ttatctttaa tacttctgga tcaaatgtgg aataatcttc
     8221 tgttaccaat cccacactaa atgccagcgt gttcattaga aaccgatcca tttcatctgt
     8281 atattgaaag ttcggtgctt tgtttgttga aataatattg agcatttact ttcctccttg
     8341 attcgtaaca cgtggtaaca cctacgtaac aggtacccgt taccactcaa tcccaacggg
     8401 gacaagggtt ctagcgtttt ggtaacaggt aacgggtaaa atccagtaac attattcccc
     8461 agcatattag ctaactattt caaaagtttt aaggaaataa ggtgttaccc cgttaccaaa
     8521 ttgctgtatc ccttggggca gtaagggtaa actagtaacg ggctaggtgt tactgacccg
     8581 ttactacccg ttactttttg tactttccag ctcgattttt attttcatag gcatttgact
     8641 tattgtaaaa tcctcgtcgt ttatttcttt ttggcagttg cttccgttcc caaccaggaa
     8701 tactgctaat aatcaaggca atctttttag tttctgcatc tgatcgacca ttcaataaat
     8761 cctttgcttg tttatcaaaa acagcttcca aaatatcagc ggtgagtacg ctatcaacta
     8821 aataaaactc ttgttgcata gtagctccat tatagaaatg ctgtaaattt ttctcattac
     8881 atttactgtc aggatagttc tgataataag aacgtctcgc ccacgctgga gcttcttccc
     8941 atttatacgg gatttccatt tccaaatatt tagtgattgc ttctttaatt ggattctcca
     9001 tcttggcatc ctcttgatag tcttttgcaa tttctaagac ttctttatct tcatcgggac
     9061 taaaatagat tcgttgtcct ttatcaaaaa gcacctttgc ttcagctaac acttgaagaa
     9121 aatattcatc ctttgtttta aacggattca tctttggctt attcttacaa ggcaaaggat
     9181 aaaaacggcg gtttcctgta tcatctttca aatactgccc atcattcgac gttccaataa
     9241 atacacattg ccttgcccaa gctatgacat tccgaccata aggaggtcta attttatcga
     9301 tcttggcact gatgaagttt tttatctgtt caattttagt tttgttcatt gaagataatt
     9361 ctccaagttc aataattaca ttgccttgaa gctgcatata atcatctttc ttttcaccta
     9421 gcgaatctaa tgaatcggtg aaaaaatcat ccgtgtaaag taatgctgga acggtactct
     9481 taccaatccc ttgcggtcca tccaaaactg gaacgatctc aaactttatt cctggtttat
     9541 agatacgtgc gactgatccg actatccaac gttttgttac ttctcgtgtg taaaggttgt
     9601 cgtctacccc taaaaggtca ataaacagtg tttccagtct aggctgttga tcccatttaa
     9661 ctttttcgat acgttctttt actgggttga aaacattctt tctagccaca tttactactg
     9721 ctttgtgtat tttgttgtca tcaaaaacta cattgtagtt ttcttcaata tatgccgtta
     9781 aggcatcctc aaaactgtct tcccattctc cagcggtacg attgatccaa ggagaatctt
     9841 ttaataggtg aatgtatccg ctaaattcgt tataagcaat tgttccagct aaattgctat
     9901 cattggttaa aatcaattct gcattcacaa cactgttact tttaatctga tcgtctttat
     9961 tgcatcttaa tcgtgacatc caggttttca attcaatgat attgtcttca cgctcgctgg
    10021 cttcgggaaa gtctaaaata ctatctaatt tatgcttatg agccatgagc tttccccctc
    10081 aaatcttttt taatcatcga tcgaataatt tgattcactt ctttatcttt tagcggtggt
    10141 tcattgtacg catttgctgt ataaatcaat tctgctaaag tttgattatc tacaccgtgc
    10201 catagtaacc aaccgcataa cttagttaaa tgatcgtttc tactaccttc gccagcgcct
    10261 tgaacaatca tatctaattt ttttgctgtc cctgtttttg ctacgggtcg gtattgagtc
    10321 tttatttctt gcggtttttt tagcagtagg ttcactagcc aatcgggagc tggtgcaata
    10381 ttatttagag atacgttttc aataggttca tatgctctcc caacacttct tgaaataatc
    10441 atagttggcg ctattggaac tcctttagtg ataatatcaa tcccgcttaa acttttacct
    10501 ttatcatcac gcccgaagat atcagcaaat gcacctgttt cttgttggat aattacacca
    10561 tcaggaattt taaagaagaa atggattcct ttacctggtg tgatttccgt ataagtagaa
    10621 ggaaatggat catacattga agcaagtttt ttataattct caacgccgtt tcgtgtttca
    10681 gaatgttgat ctaggtcaac taccaccatt tgatgatctt caagcattaa cccaatattt
    10741 ctagttgcat cttccgtcca ccattcatta atttgattag aatcgttagt tgcatcattc
    10801 caaccactag agccttttat tggaatttta gttccaggag ataagggata aattgaccat
    10861 cccgactttt gtaattccgt tgctgcttga taattatttg ccaataaacc tctcctcctt
    10921 tttcaaaata ctggtataaa tcattcagac acctccatga gcttcaactg ctcaagttcg
    10981 tatttcaacc catcccgaac aaattcagca ccttttaatt gattctgata ctttacccat
    11041 gtattaaatg ttttcaccct tgcttcttgc atatcagtaa tgtttaacca tgcggttaaa
    11101 atctgatttt ttagctcgtt gatttctcga caaattattt tgtgttctgc atgttttttg
    11161 agtacataca aaactgttag tttttcattg aatgaatttc caaagtaggt tttcccgata
    11221 ttattttcta gttcagcgat cccttgatta atttcctcta attcaaggct atcgagttgg
    11281 tttaatagtt ttgttttgta catctactta tgccctccag ttcttaattg atagtcgtac
    11341 tcgtcataag cagtgaagag atataacgct aatcctgcaa agatagctat ttttgcaatt
    11401 aatggtaata agccgaaaat tccgccgatt aaaatgcttc ctataattaa gtttcgtttc
    11461 ataatggttc cctccgattt tttaatatgg tataatcgga acaaagaata tcgggtttta
    11521 tcccaattaa gttgccttta tcctgtccgc caagactgtt atggataagg ctttttcttt
    11581 gttctcgatc atgttaatgc tcctaagttg tctgcaattc agcagaacga ttataaattt
    11641 tctcactcac tgaaaactgc aacccatatt tttctttaac agtcattaat tctactgtct
    11701 catctaaaat aggttcacga tcaaccaaca tttcaggagt catatctttc ttttttacca
    11761 ttttggaatg accataaata gttgatacag ctttattggc tatcacattc gctttaatgt
    11821 agtctactgg ttttggttca gtaatagcgt ggcttaattt agtcattgct tctttttggt
    11881 gttctttgtc tagcatgcgg aatacttgaa agccttcaag tcctgtggac tggcgtagtt
    11941 ctttgatgat gtcgaaaatc cagtcttgaa atgcctcggc ttctttttta cggcttttga
    12001 atgccatgcg gtaaatattt tttttcgttg atgatgttta catcttgtgt tctgcccaat
    12061 gaatcgatga ccttactagt agtaacccca tctttaggca aagagtgaat ggctctagta
    12121 acctgtttaa gacctaacgc ttccgcaaca tctttcgcca ctgcccacca ttcatcatta
    12181 atatcgacaa atcttataat gtgtccgttc caaatttcag ttttcattca acgtcctcca
    12241 gttcaagccc tagcaagctc gctaacaact caacttgctg atacaaaatt tcttgatact
    12301 cttcaataga aagtcgttct ttaaactctg cttgtactaa atcgttcaag ccacgcaata
    12361 ctccgcttac cgttggttct tgtggtagca atttttgata gtccatttgt tcgtacaaac
    12421 attcaaacgt ttcttctagc gctttgcctt ccaagtttgc taattctgcc gctgtgtctt
    12481 ctgtaagaat tgttgataat actttttcga ttgctttatt ttgtgtcgat tccattatta
    12541 tttcctccta ttaatccctt tgcccccacg gcttgcgttt atttttggta ttcttccata
    12601 aagcgatcac attcatcttg tgaaattctt acgactccat caactataat caactttaaa
    12661 ccgttaggaa catatttctg catcatagta ttgtatgaaa cgttcatata cttagcagct
    12721 tgtgccttgt tcatatattt tggaaactgg tacttttttt ctaataatga aagtaacgtt
    12781 ccttgagttt gctctacttg ttgaacaact cgatcgtcaa taattttaaa agctggatca
    12841 tcaagcgtga gtttgtcaat ttttaattcc atatttatcc cccctcgtta gcatacattt
    12901 ctatacaaag attttaaaaa aatgcaagag gctcttgaat acatttctat acctcttata
    12961 aactcaatat accatcatac cttttcattt gcaagaattt taacatactt ttttgaactt
    13021 ttatgtttca cacatattta aatcgtgata tagtagagaa aatatatgaa tggtggtgaa
    13081 aatgtggcta gaaaaaaata ccccgaagca gtggagacag gacaaagaat tagactggct
    13141 agaattgaaa agggactctc aatggaacaa cttggaggta aattatcccc accggccagt
    13201 aaaggtgccg ttagtaattg ggaaaatggc tataatcttc ccaataacac caggcttaaa
    13261 caactttcaa aggtattaga tgtatccact acctatctat taaacggatc ttatacatta
    13321 agagatatgc atatgatgcc cgaaattaag cgaaatgtgc ttgaaaagca attgaaaaaa
    13381 actattgaaa aaaataactc tataattgat tctaatcttt ctaatgctat caataacatc
    13441 gaagaagata acctgactgt ctcacagaag atttatttaa caaacgcaat aaaatatctt
    13501 aaaacaggat ctcgaattga atttcctgac gggagtaccc caattgctta tctcgcagct
    13561 cttctaaata cgcttttacg atatgactca ttaattaatg attcagaaaa aaatacagat
    13621 gagaaaaaag agtatcaaga attttatgaa aatgatcttc taaaagagtt cacagctctt
    13681 gttactgagc taaataaaca ctattcaaaa taataataaa ctgcctcctc caaccgcccc
    13741 cacggcttgc tacactatgg agggaaaact attatggcaa cattcaagca atatgaaact
    13801 aaaaaaggta aattttggct ctatgaagcc tatcttggaa tgaacaaaat gactggcaaa
    13861 cctgataagg ttcgtagacg aggttacaaa acaaaaaaag aagcccagct tgccttgtca
    13921 cgcttgcaag tggactacga taaaaacggt attaaaaaag cgaacaacga aacgtttaaa
    13981 gaagtttatg acttgtggct agaaacatat aaaaccactg tccgagaagc caccttcatg
    14041 aaaactgaaa tcaagtttag aaagtggatt cttccaaaat atggaaatct tcgtgtgaat
    14101 gaagtcacgg taaaaaaagc ccaacaaatt gtaaatagct gggctgaaac tactgaccaa
    14161 tacaaggtac ttcattcaac ttctaagcgt atttttagct acgctatcaa tcttgggatt
    14221 attgagacta atccactaga caacgtatta atgcctaaaa gaaccaccac taagcataaa
    14281 gaaaaggtca aagtttacag taaagaacaa ctgatcacct tgtttgatta cctaaacagc
    14341 aaacctgaaa cttatcgaaa cgactttgat aaaacgttac tacgctttct cttttatagc
    14401 ggggttagga tcagtgaagc ccttgcattg aattggtcag acattgattt tcaagaaaat
    14461 acggtcacca tcaataaaac actaagccaa tccaaaaatg gctataaaat cagtgatcct
    14521 aaaactgatt gtagtgctgg ggttcttcca ctagatgaaa gaacaatttc tatattaaaa
    14581 aaatggcaga ttaatcagcg gaaatatatg ctgacacttg gtgtaactga tccgactatg
    14641 attttttgtg gtatctataa agaactaatc acacaccacg ctatttatgc aagattgatt
    14701 actatttgtg aaaaagctat ggttccattt ctcggtgtgc atgtagcaag acacactcat
    14761 gcttcaatgc tcttggacgc tggagctact atggtagaag ttcaaactag attacggcat
    14821 acaaaaatta gtcagacgat cgattgttac ggtcacttgg caaaagaaac gaaagaaaag
    14881 actgttgaaa aattagtaca acacttaaat attgactaga tggcatacaa tatggcatac
    14941 agtaaaaaat aaaaaaagga gaaaccctat catatcaagg tttctcctag ctttatagtc
    15001 tattcttctt cttccgccat gaatgtattg aatacttctt caatcatgtc ccattctgcg
    15061 tctgtttcga tttgttctaa ctcgccttct gttccatctt cgttttcgat gtatgcataa
    15121 gcttgtaatt ctacatcttc atcttctgaa acaccagcag gatataaaag aacatagtta
    15181 cgtccaaatt cttcttgtcc gtcaactgtt agtaggattt catataatgt ttcatttcct
    15241 tggtcatcca ccaatgtgat gtgttcgtgt ccttcatggt cgtgggaatg gttatgttct
    15301 gtcataagat tacctctttt cttttattat taaaataatt gcttacctga gccgtctaaa
    15361 taattttgta agatcatcac agctgctaat ttatcgatga ctttttttcg tttggctctt
    15421 gaagtatttg cctgctctac tagcatacgt tctgcctgaa cagtggttaa tcgttcatct
    15481 tgataatcaa ctggcaagga gaatagttct ttgatctttt ctccgtatga catggaagct
    15541 tctgcccttg gtccgatgga attgttcata tttttaggta atccaactac aaaccggtct
    15601 acttcatatt gtgctactaa ttccttcaac cgatcaaagc caaactcttc ttctgcttca
    15661 ttgatccgaa tgatctctac tccttgagct gtccagccaa aggggtcact gatagcaaca
    15721 cctactgtct tagaaccgac atccaagccc attactctca taaattcact ccatgatttg
    15781 agaggtagtc cttgacaatt acttccatga tctcatcgcg ttcgtgacgg cgaatcaaat
    15841 ttcgagcatc acgataacga ggaatgtagg ccgggtctcc agaaagcaag tagccgacaa
    15901 tctgattgat tggattgtag cctttttctt ccaaagcccg ataaacaatc gctaatgttt
    15961 cgctcacttc ttttttgcga ctatcgtcaa aaccaaaacg tacggtttca tctgtaaaac
    16021 ccatgattct acacctcttt cccgcaaggt aattccagct taccttctac aaccttattt
    16081 tactcgaaga aagggaaaac tacaaacaaa tattccaaac ttccgaataa attaagaaac
    16141 actatttgta tgtttttttc cttttttctg tagctttatc aaaagtactc ttttcataaa
    16201 gtatacgcta tatttctttg aatgtatgtt tttacacaat tttttcataa tattcataaa
    16261 aaattcgaat agctgctact aattttttgt ttttgttgct tgtcctgtct cataattgat
    16321 cgtgtagcca tcttgaacat tgtagataaa gacattgaat tcaatttttt tgtcttcaat
    16381 gctttctgct tccaattgca ctcccctggc taccagttca ttcccctcaa aatgaggagt
    16441 gacacggtag cgtacatgat gatttgtttc tttgatatat gccgcaatat cattttcgtg
    16501 agcaagcatg tatggagcat tcaacgaacg tgtccctgtc atcaagtttt taatgttgtt
    16561 attttgacca gtcagctgga aaccaattaa atggctgcga ttatataacc attgcccgtc
    16621 acttaatttc ttttgtttcc aacctgtcgg gtcaatatac aaaggctctc tcttttcagt
    16681 agggaacaaa tcttttccaa gcatagcatt ggcttgtcct actctgttca atttatcaat
    16741 atttgaatag ctttcccatg ttcctttgga aaggctaaga tcctcttttg taaattccgg
    16801 ctgattccca ttgatttcta tttcttgatg ttttccatca tattctggca catccgctac
    16861 ttgagaagct tgtggtgtgg aatcaaaaat agttgtaatc tgctgaaaga tatctgaatc
    16921 tttttgtgtg gtggaaacac cgaatgcccc tgcaatcaca gcagcgacga tccccacgat
    16981 cgccttggtt gtttttttgc tcatttcttt actcctttac taacagctcc ctaaatctta
    17041 acatatgttc ccctataaat aaatactaga aagaaataga aacagcaaaa aataaaacct
    17101 gttttaagat ggcttaaaaa tacaaaaaag agacggattc ctcgtctcta atcagtcacc
    17161 actctatctg taaggtattt attattattc aaaaattttc ttcatactca agacaataca
    17221 ctgatttcac aaccgcttgg taaacggtgc gcaaaagctt gcatctgcgg taataagtcc
    17281 atccaagtta aaaacataag aagttgtttt tgcttggctg ttcttattac ttgttgcaga
    17341 gcagcttttt cacaacctat tttaagcggg tgacgggaat cgaacccgcg acatcagctt
    17401 gggaagctgg gattttacca ctaaactaca cccgcaatac aaaaacagta tacgcctctt
    17461 ttattccttc ctcaagtttt tttattcttt acgcttctgg tatgttttgt agtaaaataa
    17521 aactaatcaa tcaattattg gagaagatgc tatgacggga aaagaattag atctgttgct
    17581 ggacacttgt ttactcgctg ggaaaatcat gatggaaagc aatgcggaga tgtatcgggt
    17641 agaagatacg atgagtcgaa ttgctttagc ttctggaaat ttccgtttgg tcagctatgt
    17701 cactcaaacc ggtctcttta ttggtcttga tcgtacctcg accatccgaa tggaacaaat
    17761 taccaaccgt tcgattaatt tagaaaaagt agtaaaagta aatgatcttt cacgtaaata
    17821 tgttgcttca gagctaacat tagaagaact ttactgcgaa ctgcaagcaa tcgaccgaga
    17881 ccgttctttc tttcctgtct ggttgcaaat cgttagcgcg gcagtgatca gtggaacaat
    17941 tatggtgtta tttggcggaa agatgaccga tttacctgtg actttagtca ttggaggatt
    18001 gggatacgca gcttatctat atagtttgaa attttttaga atcaaatttt tatcggaatt
    18061 tttatcttct ttattgattg gcatagccgc tatctacagc gtacgttttg gcttaggaag
    18121 caatcaggat ctaatcatta tcggatgtgt gatgccttta gttcctggag ttcaaattac
    18181 gaacgctatc cgtgatttgc tagctggaca ttatgtctca ggagtttctc gcggaacaga
    18241 agcgatgatg actgctacga tgatcggttt tgcgattgct tttattttcc agctttttta
    18301 ttaggaaagg aacacaagta tggggacgat atttattcag ttttctttta gttttttagc
    18361 gactgcggca tttgccgtta ttacaaatgt acctaggcgt tcattgattt gttgtggtct
    18421 ttctggtgct tttggctgga tgatctactg gatctgtgtt caaataggcg gatctgctgc
    18481 ttttggttca ttgcttggtt ctttaggagt agcattcatc agcgatcttt tctctaaacg
    18541 tttgaaaatg cccgtaacga ttttcaatat tcccgggatg gttcctctag tccctggtgg
    18601 tttagcttat caagcggttc gaaatctcgt tactgaatcc tatcgagaag cggctcatta
    18661 tgcggtccaa gcaattatga ttgctggagc aatcgcttta ggtcttgtat tatcagaagt
    18721 ctttaaccat aacatccgta atttcaaagt aaagcgtgac gcaatcatga tgaaacgaaa
    18781 aaaacggctt tttaaataaa cgaagtgtaa caaagaaaag gttgtgaaag agacgttcag
    18841 ccttgagcga tgacaaaata tctcacaaac attgcgacaa gtaaccgcag gagcaataag
    18901 aagtaattat gagtattaaa aaaagttttg taaaaatagc ttctcacatt tttacataaa
    18961 attgacttaa tactgagaaa ctgacttttg aacaccctct atctgtaaaa aaggggctgc
    19021 gacatgattt ttgtcacggc ccctcttctt tattcaaatt ttttccaaaa cgttcgttga
    19081 taataaacga tccatagagc ggaaagtatt agaactaaac cgatcacagg aatattcact
    19141 aaccaaaatg gataaaacaa agccaacatt ccgtataaga cgataagaat aatactgatg
    19201 atcaatgaac ccatcatagt tgcgactaaa cccaaacttc ctgaacctct gttaaataat
    19261 tggttaatat tggtccagtt cagcagcagc aaccgataat ctctagcgaa ataacgtagt
    19321 gacaataagt agcagccgaa taaagaacca aatagcaagc tagggataaa aaacaaaggc
    19381 agctgaaaag acagtccgct taataatgcg atcccaccag ttaaaaaact ctgcaatatc
    19441 catccgaatc tgaacttttc ttttaaatat tggttcatag agattggcaa agaacggata
    19501 aacagaaaat tttcttgatc caaagaaata agattgctga taaaagaagt ctgattaact
    19561 gttaagaaag ccaatgcgat ccctaccagg aaaacaacac cgattaactt attatctaaa
    19621 tcagttaatt ttatttcgcc tcctagggca aaagcaagaa taaaaatgat tggagttaac
    19681 aaggaagaag aaagaacttg cataatcaaa ttaggttctt ttactaattg agcattgtaa
    19741 ctaaataaaa gctgatgcac attttggttc tttttatggg ttctttttat tttgaactca
    19801 ctgtttgaat cagtcagttg ctctgtaagc tttggtacta tcagctgttt tacagcaagg
    19861 aaaaatccca gagccgtgat agctagtatt ccccaaccca ataaaccttg ttgagaaagt
    19921 ggctgactgg cagcatcata gaatggcaat aatatcgaaa tcggatgccg atctgttaac
    19981 tcattttcaa tagtccccgt ttgatgattc atccacatga ttccagcaaa tgcgatcagc
    20041 atcgagctgc caagcaagat actagaaact gtttttctat gttttttaaa cactgctgtt
    20101 cgagccaaac caaaaacaat caaactagcc agacaaaaaa gaataatcaa aaatagagaa
    20161 aacaaaagga ttgagataat gatagtaagc aaaatcccta gatcagctcg ccatccggtt
    20221 aaaataaaca tgactagaag cggaaaaaca aaaggaatga tggtcaaagc aactaccaaa
    20281 atctttgctg aaaaaacctg ttcttgtcca aaaggcagag gcaaaaatac ttgcaggtca
    20341 tttccttcaa aaaagatatt ataaatgact gatatacttt gagttaaacc taacatacca
    20401 aaaagtgcca cgtaataagt aaagaaacct ggcaatttaa caaagttcat agcaaacata
    20461 gcaaatccgt aaatcagtaa aaaaatcagc cctgaaaaaa gattttgaga caaaagatag
    20521 atagttaatc gctttccctt ttttcctttc tctcttgctt tattcgtgag ctgtggatta
    20581 acatataaaa gattaactcg aattaattct aataattgtc gcttatacat acggtccatc
    20641 tcctgtcgct tgccttccag ccattttcaa ataaatcttt tctagcgttt catcaggtga
    20701 gccattcaat aattcactaa ctgccccctg ataaatcaat tcacctttct tcaaaatagc
    20761 cagtttatcg cataactgtt gtgcggtatc caatacatgt gtagaaaaaa tgactgtttt
    20821 tccttttgcg gcatgagcct tcatcatttc tttcaaatca aatgctgctt gaggatcaag
    20881 tccttgcaaa ggttcatcca atacccaaat atctggatct ggcagcaacg tgccgatcaa
    20941 aatcgttttt tgacgcattc catgtgaaaa acttgcgatt gtttcatttt gatgactata
    21001 catatcaaaa agcgctgtca attctgctaa acgcttttct tttttttgtg tgtctaattc
    21061 ataggcagct gaaatcaagt cccaatattc tccagccgtc aattgtaaaa aaatatctgg
    21121 ggaatcaggc acataaccga ttttttgttt gatactcaat cgattttccg tcaatttcat
    21181 tccatctact aaaatcgttc ccgatgatgg ttcaataata ctaactaaac ttttaatcgt
    21241 agttgatttt ccggcaccat tatgtcctaa aaatccaaaa atttcccctt gtcttatcga
    21301 gagagacaag tcttttaatg cttgtttttt cccgtaactt ttacttacat tgatgaattc
    21361 aatcatttct gtactccttt tgtttgttat tactttttat tgtacccttc gttattggtg
    21421 gagtaaatgt caaaaagagt gcataacaaa aaaggataga aacgactagg ctgctacttg
    21481 ggttgttctc ttctacagga aaacactctt ctaacaggta agatatgagt ctttgttttc
    21541 tttaaaatga ttcattttct atcttttctt gcataaaatt ctttagttta tcaatacttc
    21601 tctttcccat ctctagttgt tctccatttg caaatgttac tatttcgttt actctgctaa
    21661 tagatgtaat gttttcagta ttaatgatat aagcttttaa atctttacat aaatactcat
    21721 attcgagtga ttttttatat tttttaattg tgccgtttac gagaatctct tcatctttca
    21781 atactaggcc tattttattt ttataacctg gcatgctcat tagataaagg atttcatcgt
    21841 aatggatata gtgatcgcta ttaccataac ggacagttgt ccaccttttt ttcgtagcat
    21901 gttgaacagg atagttttct ttttctttca ataagtaact ttggatgctt tttttatttt
    21961 tttcataagt gaattctttt atcaaataac cactaggcgt aatctttcga ttaataacag
    22021 ttattgcttt attcggatga gacgttagat aaacaatttt acacttttca ttcttttctc
    22081 taagcatttc gcctagttcg ataccgctat aataggcgtt caaatcgatg tctaggaaaa
    22141 aaatatctga ggaatagatt tcattatttt taatgaattc aacaaattgt ataacgttca
    22201 atccctgaac gatctcaaat ttccaaggac ttaataaaat tctggattct tccaaaattt
    22261 ctactagcat tttttcaaaa accaaactat catccaaaat aaatatacgc gttaccataa
    22321 aaagtacctc cactcgatat tttactatca aaatcatttc atacaacttt tcaacataca
    22381 tgttaggaat aaattgtaat tctcttcaca atcttttcca acacctttga aaccgatatc
    22441 aaatactttc aggatgctgc ttctaaaata aatatcaaac aaatgaatac atcatgcaaa
    22501 aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg agctatgttt atttattttg
    22561 ttcttttaaa aagaacgata cacatactga tttttcggag cagagacttt ctcagcgttt
    22621 gtaacaacta cagatgggat ttcacgcttg aatggttgat aatagtctga ttggatggtt
    22681 ccttctattt tataccattc atcattttta accgttactc cttccggcat atgcgtcaac
    22741 aaaccaaaga ctcctgaatc tgccacacaa tggataatgc cgaatcggaa caagaagata
    22801 tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt catagctgat ttttttgcca
    22861 atgaattcgc tagggtaatt ataaattagc tccataactt ctaaatagtt ttcatctgtg
    22921 atcttgatag tttgcttatc cccatacttt tctttcaact cattcatttg gtctaagtag
    22981 tctgatttgt tgaaataaat actggtatcc ggctttaagt attgtgtctg catctccgga
    23041 tcccctactg attctttact tactgggaaa ttaaatcctt tggcttctac gatcgtagtg
    23101 tcgagactaa ctgttggaaa caacgtcccc acgattactg gcaaagctag cagtacataa
    23161 gcgattccac gttgataagg ttttgacaac ccatggtcat gatcgtgctc gtgtgccttt
    23221 tcttctgctt ttccgttttt gacccacaaa tacagctgaa ctaaagccaa aagagcagat
    23281 aaaatcattg aaagaatagc taagtagcga taatggacat tgatatattg atcgagtttg
    23341 ccagaaactt gcaagtacat catcaattcg aaatacccag ataaaatcaa aaaccgcagc
    23401 atattttccc tcctaaataa ataatgtgaa tccaataacg ctaagggcaa tcagtccgat
    23461 cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc atcagtagat ttttgatatc
    23521 gaccattggg ccaaagacca aaaaggcagc tacaggtgct gtgccgaaca gacttaacaa
    23581 agaagatcct ataaacgcat ctgcttctga gcaaagagac atcgtaaagg ccaataacag
    23641 catgatgata atggctaaaa tctttgttga gccgagcgtt agcataattc tagttggtaa
    23701 ataagtctgc atcgcagctg cgattaatcc gccaatgatc aaataacgac ctgtatcaaa
    23761 aaattcgtcg attgaatgtg tcaacacatg cccagtttga tggatcagct gattttttgg
    23821 ttcattcttc tcactttgtt cttcttcatg gacatgatga tggtgatttt ctgctacttt
    23881 agacgtgatt ttttctttca atacagcgtc tttttgaaaa taggcaagcc agacaccaat
    23941 cacaagggca acaaaaaagc tgcctatcac ccgccagctt gccatctgcc aagaattgcc
    24001 aaatgcgata aaagtggaaa aaatcacgat tggattgatg atcggagcag tcagcataaa
    24061 agcaaacgct gtgtgaactg gtacgccttt tttaacaaat tgatgaacga tcggtacgat
    24121 gccgcattca catgaaggaa agaaaaatcc taagatcgaa ccagtaaaaa tagagaagat
    24181 acgattttca ggaagccatc tcttcacacg ttctggggtc aaaaatactt gtaaggcgcc
    24241 tgagataaga cagcctaata atacgaaagg taatgcttca ataacgatcg ataagaaaat
    24301 cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac atgagtttcc tcctttttat
    24361 ttctagttca ttcgctttgc aaatatacgc ttttattcaa aaaaaacaag ttgctctgta
    24421 aatttttaca caactcttat tttttaccaa agcacttcaa ttgacttttt ctgttattcc
    24481 cattaaatta aggtctcata catatcactt ctatcataaa agggagaata aaaatgaaac
    24541 aaaccactaa cttgactgaa gttcaagaca tactgcaaca gtcatccgtt gccgtcattt
    24601 atctttcgat gcctaattgc agtgtttgtc acgctgttcg tccacgacta gaggaacttc
    24661 tcacacatta tgatattccg gcacttcatt tggatgcatt tgaaacacca gaagtagcta
    24721 gtaaatttga agtgctgact gcgccagttg tcctgatttt tcatcaagga aaagaagtct
    24781 ttcggcaagc acggttcatc gactttaaaa aaatccgtta tctactagat gaactgacag
    24841 cagaagatga ttccctttct tatgaagaaa tcttccgaac aaaataatcg ttgtccaaac
    24901 agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg ctttttattt
    24961 ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat agatcaaggt
    25021 ttgcaattca ttcgtcaaat caacattttg gatcaataca tctgacggag cattcaatcg
    25081 agcagcggta aagttcaaga ttcctttgat tcctgcttcc gttaactgat tgactacttc
    25141 ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg atttgttgga tcttgatttg
    25201 ttctgacata tcttccattg gataaacggg gataccgtct acgatgcgcc caacgatttc
    25261 ctcattcaca tcaaaagcac aactgatacg tatgctatta ctttgatgga atttgtattt
    25321 tagtaaagca cttcccaagt tccctacacc gatcagcgca acatttgtca attgatcgtc
    25381 gtttaatgtc tttgcaaaaa agtgcataag atcttctaca tcataaccat acccacgttt
    25441 tcctaattcg ccaaaataag aaaaatctcg acggatcgtt gcactatcta cttgaacggc
    25501 ttcacttaat tcagttgaag acactttttt ctttccagca ttatttaaaa tccttaaata
    25561 gcgataatac aaaggcagtc ttttagctgt cgcctttgga ataatatgat ctttcacgaa
    25621 atgacctcca cgtattgtct aattacatct ttgatttctc catcatagca gtatttatag
    25681 gaaaaaatca attccattgc tcagatattg tgaaatttta ttctttattc atttatttat
    25741 catttctgca aatctcgcag tataatggaa tttatggtag actactaatt gaaaaatagt
    25801 tttttatttg aggagaattc acaaagtaga aaagaggatt ccatgatatt attacaagcc
    25861 aatcagattg ctcgccattt tggtgcagaa gtcttatttg aaaacataca tttagaaatc
    25921 agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc gactttatta
    25981 aagatcattg ctggaattga agcacctgat gaaggaaaga tcgctaaaaa taaaacagcg
    26041 actctcggat acctcgctca ggacacagga cttgattcca atgaaacagt ttggaacgag
    26101 atgctgaaag cttttgaaca agttagacaa atggaagagc ggatgcgcga gctagagatt
    26161 ttcattagcg aaggtacgcc agacacacct gcttatagtt cttatttaaa agaatacgac
    26221 cggctgcaac acgatttttc agatcgaaac ggctatggtt atgaaaatga aattcgttcc
    26281 gtattgcatg ggtttaaatt cgattcttca ttttatgatc agaaaatcga tactttatca
    26341 ggcggacagc gcacacgttt agctttagct cgcatgcttt tgcaacaccc tgacattttg
    26401 attctagatg aaccaacgaa ccatttagat atcgagacac tttcctggct tgagaattat
    26461 ctgcaaagct atactggtgc attattgatt gtttcccatg accgttattt tttagataaa
    26521 gtagtcaatg aagtctatga attaagcaga agaaaaatgc gccattacaa aggtaactat
    26581 tcgaaatatc tagatttgaa agcagaacag ttagcaagtg aatggaaggc ttatgaaaag
    26641 cagcaaacag aaatccataa attagaagac ttcgttgctc ggaaccttgt tcgcgcctct
    26701 accacaaaac gagcgcaaag ccgcagaaag acattagaaa aaatggaccg gctggatcgt
    26761 cctcaagggg atgaaaaaca agcaaacttt ctattcagta tagaaaagcc ttctggaaat
    26821 gtagtcttgc aaatcgaaga tgccgctatc ggatatgata atgtcacctt atctgaacca
    26881 atcaatctag atatccgtcg tcaagaagcg attgctctag taggtccgaa tggtatcggg
    26941 aaatcgacgt tgctgaaatc aatcattcac cagatccctt ttatccgtgg aaaagaaacg
    27001 ctagggacaa atgtcaccat cggctattac gatcaaggtc aagctgatct gcatacaaac
    27061 aagacgatcc tagcagaatt gtgggatgag catcctacca ctccagaaaa agagatccga
    27121 agtgctttag gtgctttctt attttctggc gaagatgtag aaaaaccaat ccctctgcta
    27181 agtggcggtg aaaaagcacg ggttgcttta gccaagttag cgatgaacaa agaaaatttt
    27241 ttgattttgg atgaaccaac aaaccattta gatattgaca acaaagaagt attagaaaat
    27301 gcattgatcg attatgacgg cacactgctt tttgtttccc atgaccgata tttcatcaat
    27361 cggatcgcga caaaagtcgt cgaactctct gaaaatggca gcaaagtcta tctaggagac
    27421 tatgattatt atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt gttggcacaa
    27481 gatgagccag aatctgctcc tgctcccacg aacaaatctg attattacca aagcaaagaa
    27541 cagcaaaagc ttatccgtag ccttcaacga aaaattactc aggttgaaga agaaatggcg
    27601 cggatcgatg aatggatcga tcagttggaa caagaaatgg tcgcgccaga aaacctggaa
    27661 gatcacgtaa aactcaatga gctgaaccaa gatttagaag ctgctagaca agatcaagaa
    27721 acaaaattga cggaatggga agaactaagc ttggaattag aagaatatga aaattaacga
    27781 aaaaaccaaa acagaaacca ttcatgctgt tttggttttt ctttttttat tttatcaaac
    27841 aaaaattcca tatttacctt tttcaccaat aagaagacca attactagtc attctcacta
    27901 aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt atttacccca ttatttttgg
    27961 gcttattata aacagactgt gttaaaaagc catcttcttc ttccgttgta gcatatcttt
    28021 ctttcaattg tcgcataatt ttccaaacag cagactggta taagttcttt tctgttttgt
    28081 ctaatagacc tgattctatt aattctaata atccacaaac catgatcacc atactagatg
    28141 tatctttttc ttctctgcta gaatcatcaa agatcaaatc ccaataacaa atggtatctt
    28201 ctggcaaccg attcataaaa taattggcta atattttgct taaatcaaaa tatttttgtt
    28261 ctttcaaata acgataactt aatgcaaaac cgtaaatccc ccatgcttgg cctctagccc
    28321 aacaggaatt atcactataa ccttgatgcg tacttccata tcttggtttt ccatctttga
    28381 catccatata aaatgtatga taagttgaac catcttctct tactaaataa tctgctgcat
    28441 tttgaatatg agcctgagca gcttttttat atttttgatt tccagtttct tcactcgccc
    28501 aatacaataa tggaagattc atgcaacaat ccatgatcat tctaccttgt tgtgaaggat
    28561 cactaggatc tccccaagct tgtataactc ctgccttctc ccaaaaacgc tgaagtaatg
    28621 tttctgcagc atcaattgaa gcttccttca tttttcgatt tcccgttatt ttataatctg
    28681 ctacggttga taaagaatac aaaaaaccaa gatcgtgatg attagtgata atttgacagt
    28741 caatcctgtg gataaaatct atatctgctt tttcagctgt ttgttgaaat aaaggatcac
    28801 ccgtcttctc aaataacaac caattaattc ctgtccaaaa tccttctgtc caaccaatat
    28861 tgtctcttaa tggataacgt ccttttatag gacactcgct tgggaattga tactgactgt
    28921 tactgatacg tttgattaac ctctccgtgt gtctgcgaca acgctcgatc tcattattcc
    28981 attctttttc cattaattct ttataacatt catttatcca tttttctgga tgtattatct
    29041 cttcaaacat cactttatca agcatttccc caccagcctt tagcataatt ttagtctttt
    29101 tttattgttt ttattttgga tagttagccc ggttgtccca tcaaaatcaa ttttgggacg
    29161 attgttccat acttctaaac tataatcccg atcttgatga gcaaccactc ctgttccgag
    29221 ccaatgggtt ccttttccaa atgtaccctc taatccagga ataaaagtgt tttcccatgt
    29281 catcaagttt gtattgggca aacccataat agctgtggat tttcgtttaa tagtatcgtc
    29341 acttaaacag ataatacctg aaaagccagc ttcactcacc ctatagtttt ccttgtcttc
    29401 ttctatgatt attccttctt tctcaggaga acattcaata gcaaaccctg tctcatatag
    29461 aacatattct gtatctaatt ctactttgtg tacgcgaatc tgccagcctt caaaaggaat
    29521 aagccaagtt ctgatttttg cctgtaaagt aggttcccat gtagaggcaa gtacctcttt
    29581 cttacataca atgtctcgta cattatttcg aacgatgaac tgtccatagg ttttgatgct
    29641 ctcattctta aaactggtcg tgatatatgt gtccgctcgc tgaatcccta gcattgaatc
    29701 cattgccatt tctgctttca attgattcgc tcgcggtatc gaaaaaccaa attgactaga
    29761 ataagcaaat tttaaatatt tttcattggt caatgtatgg aaatttggcc ctaattgtcc
    29821 cgcattcaaa aaaaatgtat gtccattatc atgttgtatg agcaaattgg cttctgaaag
    29881 aagtttagtt ccttcacgaa taggcatagg catttcttta gccgtccaat actcatggtc
    29941 ttcaggtagc gccaacaata aaaaaatctt attcagccat aatggagata acgtactatt
    30001 atagggttca gtcaatgtca attgagggta ggtataacca atattcagga tacctttttc
    30061 atcaaatatt tctttttgca tccaccatct tatattccga ttgactaacc ccttgatttc
    30121 acctaattta aaaggcaaaa gatcggccca caacatagct gaccaaaagc ttgaaactgc
    30181 aaaacgataa atcatgcttc gaccaaaagg cacatttgca ccatcttccg caaaaaaatg
    30241 aatatagtct ctagaaaaat caatagctcg gttcttatat atctggctaa tgggcttttc
    30301 tggttctagt acgctataaa tcaaaccata ataatgaaat gcaaaaggat tataataatc
    30361 ttggcgacct cttgaactgt ctcgatacca gccattttga agataacaat tttctatatt
    30421 atcaaatgct tctttcagtg ctgattcttt aactggataa ccaaattttt ggataattgt
    30481 tccgacaatt atcttaaaaa attgccaatt accatctgca cattcatatt caaaaatttg
    30541 cgagaaccaa tgaataatct ggcttttttc tcctgtatct aattttttcc ataataattg
    30601 ttcatgatga tataacgcta ataacattgc gggcatttct accatacgct ggtctctttc
    30661 atgtatttct ccccaataat cgggatggga aggatctgtt ccagctttta ttcctgatag
    30721 aattaatttc aaccatcgtt catctaattg gtcaattgcg ggccccgctc cccataaaac
    30781 ccgagagaat ccttccattc ctgcaatttt atttccatat cctacccctt gatgtatata
    30841 atcaatttgt gcgccacttg ctcgataaaa cggctggagt ggttgattta tctttttaaa
    30901 taagtcaaag taatcctgtc tggtccttaa tagtggtact ggatgtttca ttgtattttc
    30961 tcctcttttt actaccaaat agctaattcc gactgttcta atcgaagtaa gccttcaata
    31021 aaaaaataat cagaatagat cagtggcaca ttataagtca gttctctatg gtatgcttct
    31081 gctccgcctc caatgatccc atcttcatcc atattccaat ttgcatagtg cttttctgtc
    31141 ttttggagta tattgatagc agcttcatga tacatttttg atcgtacaga ggaaacatat
    31201 cctgctaatt ccagaagacc gcatgcagcg catactccag cactggtatc aatattcgaa
    31261 tcttttacag caaaatcaat gagaggaata taaccagttt tttcgatttt ctgaataaaa
    31321 tttactgcta tcttttctgc tatagctaga tagtacggat tttttgtgta acgataactt
    31381 aacgcgaatc catataaagc ccatgcattt cctctgctcc aacaagaatt ctcattgtat
    31441 ccttgcccgc caagtaaccg aatatatttc cctgattctg catcaaattc tgcgatatgc
    31501 ccggttgatc catctttcct cactagatac ttagctgttg tatctgcatg ctgccgagca
    31561 attttagaga acctcggatc accagtttcc ctcgaagccc aatacagtaa tggtaaattc
    31621 atcatactat caataatcat ccaaccaggt ttatggtcat tccatgctac taaatagttc
    31681 cctgaaatat tatagcgtcc agcaagtacc gtagctgcat gtaaccctag ttgtcgacta
    31741 tgtttgtttt gagactgttg ataatgtgca atagccgatg gtaagaataa aaaacctaca
    31801 tcatgatgaa cgtctgaaaa atggtacagg gcagattcta ataattgttc ttgttgtatt
    31861 gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa aataccactc
    31921 caaaaaccat tggtccacca gtcgattcct ctgtccttta ttacgtctgt atattttccg
    31981 ttgtaagaaa tatatggaat tttattcccc attctgtcta attccgcatc tatcttttca
    32041 gaagctacat tcaaaatttt tcttgaccat tccttcgtct gttcattcat ggatatcccg
    32101 ccattctttt aatttttatg aatgtggagt tgctcctaga acgaactaga tggttaacat
    32161 tcgttatttt ttttacttat ttgcagctcc aatgtcattc tccttaaatt tagactgcgc
    32221 ccacacataa atcaagacca ttgggatcac tgaagttgct aaaattagca taattgcgcc
    32281 ataattaatt ggctcacctg tactagaaac cgtcatattt ttgataatta agggaaccgt
    32341 atatttatct ttatctgaaa gcatgactaa tggaaataga tattgattcc aacttcccat
    32401 aaagataagt aaaccactag taataatcgt aggtgataaa atcggtacag cgattttcgt
    32461 aaaaatcacc cactcattgg caccatcgat tctggcagcc tccatcagtg ctgtaggaaa
    32521 ttgactgcta tattgtcgca tcataaacac tgaagttgta ctggcaatcg aagggatgat
    32581 cacacctgca taagtattcg ataatcctaa acgactcata atgtaaaata aaggaatcaa
    32641 tgtggtaaaa aaaggcacca tcctagatag cataactgta ttgaacaaca gtttattccc
    32701 cataaaacga tactttgcta atgcataacc cgccattgta gaactggctg tagctaaaac
    32761 tgtactgatt acagcaataa caatcgaatt ccaaataatc aagggataat tatatctcgc
    32821 catgatggct tgccagtttt ttaatagcat atccccaaac ttgaaattga ttccgccact
    32881 taaaatttct tgattacttt gcgttgaagc tacaatcata tataaatatg gcatcaaaaa
    32941 tattaaggca ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta tcttttgttt
    33001 agtcattttt atcacctccg aatttaaatt gaatagccga taatataaca gatacaaaca
    33061 ccatcgtcca ggcaatagca gaggcttttc caaaatcaac catttcaaaa cttgttttat
    33121 ataaattgac cctacgacca aagctgaatt ttctggtcca ttggaagtcc caaagaatat
    33181 atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg ccattaaaat
    33241 gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg atgctccatc
    33301 tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta aaaataaaat
    33361 agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc gtatccaaaa
    33421 aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc cattgaataa
    33481 cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg atgtaatggt
    33541 tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg aattcaataa
    33601 aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca tgataatcaa
    33661 aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat aattgtcaaa
    33721 tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg agataatgat
    33781 gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc cataagcact
    33841 ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct tgcttggtaa
    33901 cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt aattttacgc
    33961 cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa tcacctgttt
    34021 gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa gtggctggta
    34081 aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat ttttgtcctc
    34141 cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag ttagatgtag
    34201 ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata aatgtaagaa
    34261 attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt gatcctcctg
    34321 atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc atgcgccatt
    34381 tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg ccagcaacaa
    34441 acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa tgtacaatat
    34501 ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta gtattcagac
    34561 ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg ttgataaaca
    34621 attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt tcttttaatg
    34681 cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct acacctattt
    34741 cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta gcccaaggaa
    34801 taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca taaaactgat
    34861 cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct tgaaattttt
    34921 ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata tcttcgtgtg
    34981 ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca ttcacaattt
    35041 ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt tcaacatagt
    35101 ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt atcattggat
    35161 ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag ctgaataata
    35221 gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct atcccttctt
    35281 tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa ttagtaacac
    35341 aaatatttat ataaaaaaat attttacttg tcgttttggc ttttcatttt tgttataata
    35401 gtcattaatt ataaatagaa agctttttat ataaaaagag aaaggaaagt ggttcttttg
    35461 ttaacaaaca accaatcctc caatcttgtc aaaaaaacac taacctattc catagatacc
    35521 catcttcatt tatctgctgc tccctatttt cgactactaa taaatgaaag cgctaattta
    35581 tatgctgaat ttgcacataa atctatagaa ctagataatc cttccctttt attcattgat
    35641 aaacagcaag aagtccaaat cttttctact caaaaggaaa cacaaacttt tatcattatc
    35701 cagttttatt ttgatgagca attattagca catattacgg gacaatcttt ttctatagat
    35761 caagaaataa catctatcat tacttttctg aaaaatgaac agttcaaaat tgattatcta
    35821 aaacatagta agccagctat ttcctatgat caggaaatca attctttttt catactatgc
    35881 caacagatga gcattcattt gctttcctta ttagttactt attgtctgca aaaaattcaa
    35941 ttgtcttcct taccaaggca ttatcaacag gaacttgctt tattgacaat accttcaaat
    36001 gagaaagtca ttcctaaaaa aaagcatatt ctttattcag atgaattagt tgatccaatc
    36061 atacgatatt tagaagaaca tctttcagaa aattttttgc ttgaacaatt agcccaaatt
    36121 ttttttatca gtccagccag cttaaaaaaa cattttagaa aagtgaatgg aaaaagtgtt
    36181 attagctatt ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct agaaaatgaa
    36241 ttaacctata cagaaattgc agatatatta ggatttcatt cgatccatca tttttcttca
    36301 gcttttaaaa aatatacagg gttatcccct tcaaagtatt atctatcctt aaaatagaaa
    36361 aaaattttta ggttttatcc acaaattatt tctctatatt ttctgtctat tcaaaataaa
    36421 caataatttt ccggcacaat tatttgctgt tttttaccat aacatagtac gatcaacgta
    36481 gaaaaggaga tgtttttatg aaagttgttg tcattggcgg tgtagctggc ggtccttcat
    36541 ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat cattatctat gaacgcggag
    36601 aaaatatttc ttatgcaagt tgtgctttgc cttattattt aggaggtgtg atcactgacc
    36661 gtgactcgct gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac atcgatgtct
    36721 ttactaaaca cgaagtaaca gcaatcgatc cttctactaa gcgattaaca gttaaagacc
    36781 tatccacaaa tgaagaaata aaaacagatt acgataagtt gatcatctct tctggtgcta
    36841 gaccagatta tccggatatt cccggagttt ttgaagcaga aaacggcttt gtcctccgta
    36901 gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca caacatgtcg
    36961 tcattcttgg tgcaggtgtt atgggtctgg aattagctga gaatttcaag catcgcggct
    37021 tagacgtgac tttaatcgat caattgccac aagtcgcttt cccttatgat ccagaaattg
    37081 ctaatttagt ttatgacaaa ttgctgaaag aaggattagc cgttcattta gaaacaagag
    37141 ttactgagat ccgtgataaa ggtcgagaaa tagtattatc agatggctcc gtcctttctg
    37201 ctgatatgct aatttttgct gttggtgttt ctccgaagaa tgaagtggtg aaagcaaccg
    37261 gcatacaatt atcagataca ggtcagatca ttgttgatga ccagttacaa accaatctgc
    37321 cggacatcta tgcgattggc gatattatcg aaacgactag tgtagtgact ggtcagccga
    37381 tccaaagtat gctttccagt gcggccaatc gtcaaggaca tatgctggca gatattttaa
    37441 atggtacgcc tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa atctttgatc
    37501 atacagcaag ttatgcggga atgacagaac atgaattaaa agcatcaggc ataacaaatt
    37561 ataaaactgt ttttatcact ccctttgacc atgcctattt ctatccagaa gctacaagat
    37621 taaatctaaa gctgattttt gatgcagata gcggtcgtat tttaggtgga caagcatttg
    37681 gagaaaaagg tgtcgataaa cggatgggag aactttctgt agcaatcacc ggaaacttga
    37741 cagtctttga tttgcctgat ttggagttgc cttactctcc accgtattct actacccgtg
    37801 acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc gtagaaacga
    37861 tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc gttcttttta gatatacgtg
    37921 aacctaataa agcaccttct ggcagtatct cggcaacaaa aaatattcca atgaatgagt
    37981 tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt ttacattact ttcagaagag
    38041 gattgaatac ttatacttct gcccgaatct tggcaggttt gggtatcaaa gcgattttga
    38101 ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc taggagttat ttttacttga
    38161 ggagtaaaaa tgaaaaagta tatagggttg ttggttggta tgtatataga atagaagata
    38221 aatgtgaaga aattgtgcat tatttcttca catttattag gttatagttt aagaaagttt
    38281 aaacattgcg caatttaatt attttagctg tttcttttca taaaaaaaca tgttgtgact
    38341 ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa tattacgagg
    38401 catcatatta atctatcagc actagtacct aaactagcat ccctttcgaa tattttttct
    38461 tatataataa agtttgcttt cactcctcta atctcaccaa atcgactggc ttgcgttgtg
    38521 tgatactagg aactgattgg tttagaacga cttttctatt gtaatcatat ttttcgccat
    38581 cctcgttgtc ttctaatcct tcaatgaatg atccctaatg tgtgtattaa ataatttaat
    38641 ttttttgttc ctatttattt aaca
//
LOCUS       DACIRY010000012        37819 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.012, whole genome shotgun sequence.
ACCESSION   DACIRY010000012 DACIRY010000000
VERSION     DACIRY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.012"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(170..1480)
                     /locus_tag="IYE22_000502"
     CDS             complement(170..1480)
                     /locus_tag="IYE22_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ9505737.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1492..2910)
                     /locus_tag="IYE22_000503"
     CDS             complement(1492..2910)
                     /locus_tag="IYE22_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9505738.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3150..4718)
                     /locus_tag="IYE22_000504"
     CDS             complement(3150..4718)
                     /locus_tag="IYE22_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ9505739.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4722..5132)
                     /locus_tag="IYE22_000505"
     CDS             complement(4722..5132)
                     /locus_tag="IYE22_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ9505740.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5129..6052)
                     /locus_tag="IYE22_000506"
     CDS             complement(5129..6052)
                     /locus_tag="IYE22_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ9505741.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6049..6927)
                     /locus_tag="IYE22_000507"
     CDS             complement(6049..6927)
                     /locus_tag="IYE22_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ9505742.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6914..8251)
                     /locus_tag="IYE22_000508"
     CDS             complement(6914..8251)
                     /locus_tag="IYE22_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ9505743.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8254..9357)
                     /locus_tag="IYE22_000509"
     CDS             complement(8254..9357)
                     /locus_tag="IYE22_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ9505744.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9431..10939)
                     /locus_tag="IYE22_000510"
     CDS             complement(9431..10939)
                     /locus_tag="IYE22_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ9505745.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10905..11597)
                     /locus_tag="IYE22_000511"
     CDS             complement(10905..11597)
                     /locus_tag="IYE22_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ9505746.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11619..12593)
                     /locus_tag="IYE22_000512"
     CDS             complement(11619..12593)
                     /locus_tag="IYE22_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9505747.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12893..13765
                     /locus_tag="IYE22_000513"
     CDS             12893..13765
                     /locus_tag="IYE22_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9505748.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14223..16421)
                     /locus_tag="IYE22_000514"
     CDS             complement(14223..16421)
                     /locus_tag="IYE22_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9505749.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16653..17645
                     /locus_tag="IYE22_000515"
     CDS             16653..17645
                     /locus_tag="IYE22_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9505750.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17705..19126
                     /locus_tag="IYE22_000516"
     CDS             17705..19126
                     /locus_tag="IYE22_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ9505751.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19119..20618
                     /locus_tag="IYE22_000517"
     CDS             19119..20618
                     /locus_tag="IYE22_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ9505752.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20657..22051
                     /gene="uxaC"
                     /locus_tag="IYE22_000518"
     CDS             20657..22051
                     /gene="uxaC"
                     /locus_tag="IYE22_000518"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ9505753.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22210..22893
                     /locus_tag="IYE22_000519"
     CDS             22210..22893
                     /locus_tag="IYE22_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ9505754.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22895..23731
                     /locus_tag="IYE22_000520"
     CDS             22895..23731
                     /locus_tag="IYE22_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ9505755.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23742..25496
                     /locus_tag="IYE22_000521"
     CDS             23742..25496
                     /locus_tag="IYE22_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505756.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIFNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRIYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25772..26113)
                     /locus_tag="IYE22_000522"
     CDS             complement(25772..26113)
                     /locus_tag="IYE22_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ9505757.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26181..28352)
                     /locus_tag="IYE22_000523"
     CDS             complement(26181..28352)
                     /locus_tag="IYE22_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ9505758.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28563..29420
                     /locus_tag="IYE22_000524"
     CDS             28563..29420
                     /locus_tag="IYE22_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ9505759.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29491..30348)
                     /locus_tag="IYE22_000525"
     CDS             complement(29491..30348)
                     /locus_tag="IYE22_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ9505760.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30333..33035)
                     /locus_tag="IYE22_000526"
     CDS             complement(30333..33035)
                     /locus_tag="IYE22_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ9505761.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33048..34337)
                     /locus_tag="IYE22_000527"
     CDS             complement(33048..34337)
                     /locus_tag="IYE22_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ9505762.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34487..35533
                     /locus_tag="IYE22_000528"
     CDS             34487..35533
                     /locus_tag="IYE22_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9505763.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35535..37688
                     /locus_tag="IYE22_000529"
     CDS             35535..37688
                     /locus_tag="IYE22_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ9505764.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11170 a         7789 c         6724 g        12136 t
ORIGIN      
        1 ggttcttttt aacgacttag ttatcttatc acgtctaaaa acatgtgtca actctttttt
       61 ggaattttca taaaatgttt caatgaagcg cgacaacgtt gtttaatata tcattaaaaa
      121 aaataaaggt caagctaaaa tattatttaa gcttgacctt attataattt caacgattgt
      181 caaaagtatt gtttgcagct gtgttgccag tttctataga taaatcacag cttttgaaac
      241 ataagttttg tactgcttcg attcgcccga attcttttgc agttatttgg caatgagaaa
      301 agcagacatc ttctataggc ttttcaggaa atgctactaa atcaaaagct cgggaaggaa
      361 gtgagtaacc tggagaaagg catgcctgta cgtttttgat gtgtatgtct ttgactttcg
      421 tcatttgctt tgattcaggt atttcttcag ccactgcttg ccaactttct ggcatttgag
      481 caaggtcatt gctatttttt ctattgtatt cattatgcca gtccattatc caagaaaaag
      541 gaaattgtac atcaatcata ttcagatttt ctaccctgat attttcgatc actccccctc
      601 tttcttttga agatttcatt cgaataccac agtctgactg aaaaaaatcg atatcgtgaa
      661 tatatacgtc actaactcca gcactaactt cgctaccaat agttacaccg tatcctgaac
      721 ggattttaca tctagcaacc tcgattcttg atgattttcg attgactctt gcaccattgc
      781 catctctgcc agacttaata gcaatacaat catcaccaca ggccaaatca cattcataga
      841 cacgcacatt tgttgaagaa tcaatatcaa tcccatctgt acttggtcca tcattgttgc
      901 ggatgattac tttttctact aatacatcgt tagaataagt gatttgcaag ttccaaaaac
      961 ctgatttctg aaaaatcaaa tcacgaacaa caacatgttc tgcatgatac agtaaacaag
     1021 cgcgtggacg tttaatagca taatccgcaa tccaacgaag tcccttttga tcgtattcag
     1081 ctcttgtccc gcttttttga tcttttcccc aatacagctc ccaccaatgg tctccttgac
     1141 cgtcgattat tccttttcct tctataagaa tatctttcgc cgagagaaca ttcaaaatcg
     1201 ctgctggcca tttcatttct acgccggcta ctcgtgtatc gatttctgga aatgcttcga
     1261 tatctgttct ccccttgagg actgcacctt cttcaaagat aagatgactc ttatctttta
     1321 aaaaaagggc tccgactaaa taaatgcctt ttggaataaa tacaggtaag tgttctttcg
     1381 caccttgatc gattgctttt tgcagtatta ttgtatcatc ttgttgagtg tcgtttgttt
     1441 taaagttcaa agcattgata atcccttttt ttgtccgcaa tacgtttctc cctatctttc
     1501 attgattttt ttcaattctt caccaataaa tacagttttt gaacgattca gtgggtaacc
     1561 tactagcaaa atgattgcgg atacaaataa gcagaatgct ggtaacaagt ttgccatcgt
     1621 ataaatcctt tcttgtacta aaactgattg gatggctcca cctgttgtag acgattggta
     1681 accaatgagt gccagcataa atccgcctac cgctcctgca catgcttgtc ctacttttct
     1741 cgcaaaagaa tttactccat atactgttcc atcttctctt aagcctgtct tatattgatg
     1801 ataatcaatc acgtcagtaa tgaatgccca gaccatcaaa ttgaataaac cagatcctaa
     1861 agtagcgata aataaaagaa tcagatatac ccatgcattt gttatatgaa ggaaatacat
     1921 cagcaagtac atacatgaag aagcaagtaa cgcaaatacg cttgcttcct ttttaccaaa
     1981 taattttgtc agctttgtag caaatggtgc taaaactact actgttccgt aagtaaataa
     2041 aagagcaaca gacattgctt gtttattatg gaagtaatca ttgaataaat aagtcatatt
     2101 cgtcccagct aaaatctgat tgatcacaat gaaaatgtca acaatgacta aaataatcag
     2161 tgctcgatta gctaacaaac cttttaccaa ctgagaagca gatacttttt cgtttttgac
     2221 aactcttact ctttcagtag tcaatttaca agttaacata tatgcgacaa atgctattgc
     2281 agcacatccg attgaaatcc aaaagaaatg attccctgaa acgacttgtt gtccactctt
     2341 gttatgggta tacattaaaa tcggaatgaa gaatcctgta gttgatccgc caatcgcaga
     2401 accaatgctt cggaacgtgg acaacgaggt gcggtcatta ggatttgagc taattgctgc
     2461 tgccattgct ccatacggga tattgattgt tgagagaaaa atcccataaa caatgtatga
     2521 aacaaaaata taagcaatct tcaccgtcat tggaagatgt tggacgaatg gcaaaaacaa
     2581 aatgacagtc actaagcaaa atggatactt catttttctg atccaaggaa taaatcggcc
     2641 agtttctgtt aactctgaga catcacataa tcttccaacc atcacatcag caaacgcatc
     2701 caaaattctc gaaatcaaga ataacgtacc tacgattgcc cctgaaacgc caagaacatt
     2761 cgtataataa atcatcaaga aactattgac taaacctaaa ataaaacagt tacctagatc
     2821 accgaacatg tagcctatct tatcggaaat cccaaattcc tttttttctt tttttattgt
     2881 atttgctgta acagcatttt gtgaaagcat ttgaattcct cctacctctt ctagtaaacg
     2941 ttttcaaaat tataactctt tttttcttgt ttttatattc acaatgttgg ctttttattg
     3001 cattatcttg acagataaaa aacaaaaaat aatctaaaaa cattgatgca taagattgat
     3061 aacttttttt acataacaat atatacaaac aaaaaaattc actaactagt gaaaataagt
     3121 tagtgaacct catttattaa agaattaaat tacagatgat cttttccatc atacttgaaa
     3181 aactcaaaat cagcataact tcctcttttt ttatttagat catgcactgc tatcccgata
     3241 aaatttccag taaaccctcc tgagagtata cacatgtttt tttgcggacc tactttcttc
     3301 cattcttcag ttgatacgtc tttcaaatga aactgtgctg tctctttctt tacagtgact
     3361 ttcaaaaaag tcttcttttc tgtgagtgga atcgtccaat cttctatttg tgctactccg
     3421 ttttctgatt ctatcatacg taggcatttt cccttgtcct cattccaagt aacataacaa
     3481 taaatataat tggattcatt taagtacagt accagtccag ccatctgatt aaaattatta
     3541 ggcatgtatt caaatgctgt ctccgcatca aaagtaaaat ccgtttgccg aatagctaac
     3601 aaatgatgtt cgaaggtaga ttgaagagat tcaccggaaa tcagacgtaa aaaacctggt
     3661 ctacattttg tatcacacca atcagcattt ggcaaaatgc gtctggcatt ccagttcttc
     3721 tttagctctc cttcaaatgt atccataaac tgatgatctt tttcttgttg tacctcttca
     3781 tttgtctcaa tcattatttc ttccatcgga gttgtctggc tatctttaag tcttaaccaa
     3841 tcgtcggacg tccaatacac ttcttgaata gcggtttctc ttcccaaaat agccgcaccg
     3901 tttaatggtc tggtacaaag atatgtcatg taccatcttc ctgacggggt ttgtactaag
     3961 ctcccatgtc ctgtacattg caatgtagaa gcaggtttgt cactagctgt cagcatagga
     4021 aaaccaggat caagctcgta aggaccagta atattctttg ctcggcaaac tgtaactgaa
     4081 tgtcctcttc ctgtaccgcc ttctgccgtc atcaaataat aatacccgtt acgaaaatac
     4141 aaatgtggtg cctccgtttt cgctaattct gtacctgcaa atattttata gatgggacca
     4201 tcaagcttct tcttctctgc atcatattct tgtaatacga taccgctgga tttattcggt
     4261 gtgtccagac gataatccca tagttcattg acaagccatt tttttcccgt ttcaagatca
     4321 tggaacaaag aaggatcaaa gccgttacta tttaaataaa ttggttcgga ccaaggacct
     4381 gtgatctcag atgcagtgat caaataattg tggacatctt taaacggacg gcctgcactt
     4441 ttgacatctg tgtaaacaag ataaaacaat ccatcagcat aactgatctg tggcgcccat
     4501 atacttccat ttgtaggatt tccttgcaaa gatacttgag tggtcaaaat atccgttgta
     4561 tgtgaccagt tgactaaatt tttcgactca tatactcgga ttcctggaag ccattcaaaa
     4621 gaggagacaa taatgtaata ccgctctttc actcttaata tattaggatc tggattgaat
     4681 cctcttaaaa taggattttt cactttgata gtctgcatga ttcaggctcc ttttctcatt
     4741 cttactcgat ataaactgaa tgccccagct gcgaaataaa aaaatgcaaa aggaaacgca
     4801 ataaactgag tgccaatcaa caatatgaat cctgttataa aatacaaacc agtggcagtt
     4861 gttaagtatt ttcctttagc agtcaaaaac aatgctactg ctaaaaaagc aagtgaaata
     4921 aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt attttctaaa
     4981 gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc tgacgcagac
     5041 atattactta aagtaactgc taacccgcca aagaaaatca aagatagact gccaccgacc
     5101 attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt tctaaatgta
     5161 tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag aatttgacag
     5221 tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca tgcccaccta
     5281 tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct aaatagacgt
     5341 ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct gttttttgat
     5401 ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag ctatggatat
     5461 gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt ccttcaatcc
     5521 agcaatattc ataggtctga cgacaatact caggattggc agaagccggt gtacaaaaag
     5581 gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc tgataaccag
     5641 atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca acagcctgtc
     5701 ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat acgacattcg
     5761 atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg tcaagatact
     5821 cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca agtttaaaat
     5881 tggataatct gctttctata tattcatcat atcttccttc ttttactatc aagacctttg
     5941 gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt tcaaaatccg
     6001 tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc ataaatgctc
     6061 ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac cgcctgttaa
     6121 agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt ccagcccttt
     6181 tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa ctaagctaag
     6241 acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg tcaatagatt
     6301 tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa gtatttcgat
     6361 gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg ctgtcaccca
     6421 ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag tatctgtgtc
     6481 ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca ttccccataa
     6541 cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac ctccgcgttc
     6601 ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca gtacttctgc
     6661 aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc cgactgaaaa
     6721 atctccgccg tgagaatgca ggtaaaggac cggcggaaac ggtccttctt tttctggata
     6781 agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt attcaagcat
     6841 aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc tgctagatgg
     6901 agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc agttcatcca
     6961 tattttctga ttgatttgat tggaatttct caatactgat ttctttagta ttttctaata
     7021 gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa
     7081 agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata gcaggaacac
     7141 cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt tcttgtatga
     7201 attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt gccgtgatat
     7261 tcgaaaaatg gatccttctg aatgctggtg tccgttcatc tatcggataa gccttttttt
     7321 cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc agtataaatg
     7381 gacacatcac attatccatg actatattgg atactcgaat atcttcaacg atcccgcctc
     7441 gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata caatttgaaa
     7501 tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg ccatgtccat
     7561 ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg tacgtatctt
     7621 cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa ttactaatac
     7681 gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc gctgggttca
     7741 agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc gtccaacttg
     7801 gtgactggat caatttaata tctttcacag taattcgatg acagttatgg aaactcatca
     7861 atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc caccattttt
     7921 ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca ttttgagcat
     7981 agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga acaacaggat
     8041 aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga agttcgacat
     8101 tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact acggtcccgc
     8161 ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc aattcatcaa
     8221 ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt caatcaatgc
     8281 ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac tgatgtagta
     8341 agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac agtttttatt
     8401 caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc cttgatgtga
     8461 tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta ttcctttagc
     8521 cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt tccgttttgt
     8581 ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa gtaatgcagc
     8641 cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat gaagtaattc
     8701 ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa aatgatggga
     8761 tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg catgaatcag
     8821 caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc tcaaaacatc
     8881 ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag gagaacccat
     8941 atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa aagctccttc
     9001 ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt ttttatattt
     9061 ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta catggtcgat
     9121 attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga aatagtcgat
     9181 attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa gtaattggaa
     9241 ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact tttcttcata
     9301 taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt tttccattat
     9361 ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt atttgtccca
     9421 atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt gtgattcgat
     9481 cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg tcaagctgtc
     9541 ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat cctacttcat
     9601 tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct tcattttctt
     9661 tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag aacgtcactt
     9721 cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc atatcgataa
     9781 attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg ccagtcatca
     9841 atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc ttcaattgtg
     9901 cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca tttccttctg
     9961 accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc actaattcca
    10021 tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc attccagtgt
    10081 agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa
    10141 agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa gctttcatat
    10201 actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc cagccatttg
    10261 gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca ctaggcgcac
    10321 gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt tctgtaaatt
    10381 gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct agattatcca
    10441 accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc ggtacgccgt
    10501 acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga cttaaatttt
    10561 tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac gcattgcgga
    10621 cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt tcatcagaag
    10681 ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg aatgtcagtt
    10741 ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt gaagcatgat
    10801 gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa gaactgttac
    10861 ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga cctaatttta
    10921 cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt tttgttttct
    10981 tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga atttgctgtc
    11041 cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc tggcattgtt
    11101 tggatgatca tcagataaat ccctaccata cttaatatca acactaatga gtaatgaaga
    11161 tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt ccaatcataa
    11221 tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc agcaatatac
    11281 aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa aacaatcccg
    11341 atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt aaaatatgtg
    11401 aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct aatcactgca
    11461 accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat gactaaaccg
    11521 ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc gtttatttta
    11581 tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc gtcttctcct
    11641 agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac ccctttaaac
    11701 aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt ataaatatag
    11761 gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta catatgctcg
    11821 aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat tactggacga
    11881 attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc catttctgca
    11941 gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat cgtagaccag
    12001 cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc aggggatgtt
    12061 agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac ccctccgtca
    12121 acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa gtgagggatg
    12181 taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt taatagtaaa
    12241 gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa gatcaacgta
    12301 tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg
    12361 acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc aataacgagt
    12421 ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag aatcatcaca
    12481 tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct gagcttttct
    12541 tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc cataagcccc
    12601 tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta tctgaattac
    12661 gtatataatc atttttaccg taagtaaaaa cgcttacata gcaatgattt ttgtgctata
    12721 caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct tctatttttt
    12781 tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg atgataaatt
    12841 gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct atatgaaaaa
    12901 gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc taattttgct
    12961 agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg cttctacaca
    13021 agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag acgcgtatcg
    13081 ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg gtgttacagg
    13141 tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa
    13201 gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg
    13261 ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt cttactgggc
    13321 catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa actttttcca
    13381 agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag attttggtgt
    13441 atttttcaaa attatgctgc cattagcgtt accgtctatt gctactatct cgctttttta
    13501 tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg attcatctaa
    13561 atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga tgcaagcaga
    13621 aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg ttatcgttat
    13681 tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg tcaaaggagc
    13741 actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag ttgtgtcaga
    13801 agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa atttttgtga
    13861 aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag aggttgtgaa
    13921 agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac gagtaaccca
    13981 caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa attatgcctt
    14041 attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg tgacaagact
    14101 tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata aatacaaata
    14161 ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa cagcctcttg
    14221 attcacaaat atttttttcg atattctccc ggcgtaatcg attccttctt tttaaagaaa
    14281 cgaataaaat tttgcggatt tcgatattgc aatctttctg caatctcttt tacggagagg
    14341 ttagtctctt tcaaccattt ttttgctacc tctaaacgat gattttgaac aaaatcacca
    14401 aatttttctc ctgtttcctt tttaaataca ttacttaaat agttagggtt ataatgcaac
    14461 ctatcagcga tcacatctaa agacagatca cgatcatatt ctgtgtagac gatatgcata
    14521 atattctccg acaaattttt aaattcatga tcagtgattg cttgagagca ttctgtgatt
    14581 ggcagaacta attgttctaa aacaagtttt tctagttctt tgggatgata attgttcagt
    14641 gcttttacat aaatttgttt catgttatca aagttttttg aatctactcc caataactgt
    14701 cctaattgaa cgagttcatt gatcaagcgg accgaaacta cttcccgact caaaggattt
    14761 ttattttgtg caaacaatgc atcaatcaat tcatgtacta aattcttcac ttcctgattt
    14821 ccagaacgaa tggcttcaaa caatcgattt tgttcttcta tcggatatct aataagtgtt
    14881 ttttcatgag cagcaggaac gatttcctga taaaaaataa ttgaacttgg accagtattt
    14941 acacgataat acagggcttc ttttgcccga tcaaccgatt gcttgctttc attcagcgtt
    15001 tcgaaccggt tactgatgcc tatactgata gttaaattca agtattcttt tacacgtttt
    15061 tgaatgagtc ggcaatactc catgactttt ttgcttgctt ctgcatcatc tttatccatt
    15121 cgataaatag tggcttgcat ttcttcattc aaaacgatcg gcagcattcg attttccttt
    15181 ggcacaattt ccgtgatcat gttgttgatc gctaaaagta gcaaatcacg ctccccaccg
    15241 tgactatctt ctaacagatc gatttgaacc aaagctgtat aatagcagtc attttgagaa
    15301 ctataaccaa attgctgcaa ccgctgatct agttctcgcc tttctactcg atttctaaat
    15361 aaactaagta caaatagcgt ttccagctgt ggcttttggg taactaagtg tgcacttaac
    15421 gcttcttttt ctcctatgat ccgactgact gattgagcga ccaaatctag ttctttccca
    15481 ctgaatcctt ttgatttaag attcagacgt tcctgtattt gagaaatcgg acgggcaaat
    15541 ctgtccgaaa ataagtagga aagaagacca atgagtccta ccagtgttaa agacactgca
    15601 actagcccga ttctcagatt gcggatcatt gcgccaattg cactagagtc aacttctaat
    15661 ctgtaaatcc aattattata atctgatttc agtactatat acgttttatc tttttctctt
    15721 atcactttca gctgattgta atctagatcc acgtccgtta atacagttgg ttgctcttta
    15781 ttttttactg tctgagtagt aaaaaggttg ccaacttcgt tttcaataga aagtatgtct
    15841 tctccacttt caccaacaac ttgttctatt gtatgtttct ctatcgtagc aattcccaat
    15901 gcatattttt ctttttcaaa cattggcaaa gaaatcacca tttcgattcc tttgtttttc
    15961 ttttgccaaa ataaattatt tttgttattt atgtacttct gttgatactt ttcaatttcc
    16021 tcttcggtca gttgactcaa cgatccattg attactcccc atttaccagc caaactgact
    16081 aaatcataat tattatcatc cataacaatc gtttctataa aatttagttc tttcctgatt
    16141 tcagaataag tggagtaatc tgtatatgtc agcggatgct tgaatatgtc cgagaaattt
    16201 ttggtcgaac catacgtaga aaacgaatat tcaatcgttc taattttcca ttcaacgtta
    16261 gttttaactt gcctgagcaa attctttttg tctgttaaat aactcgattc gatcgattca
    16321 taggtattct ggtaaatgaa taaactaaat cctatgacaa gtacggctcc taataaaaac
    16381 atcggtaaaa atattttata aaatactgta cttttcttca ttttcttcct ctcctccttt
    16441 taaaataaaa ggctttcatt tttctgtaaa attgatactc atctaattaa atgagcattc
    16501 cccgctttca agaatagcat aattaaaaat aaaaatccaa gtaattttta acgtgtgcaa
    16561 tcttttcata tcaaagatct attgacttat cgtagttatc gtaatatgat gaatgcgtta
    16621 acaaaaagtg aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat gtcgctaaag
    16681 cagctggcgt ttctcatacg acagtttctc gtgctttgaa taacagcagt ttaataaaaa
    16741 atgaaacaaa acaaaaaata cgacaactag ctaaggaaat gaattattcg ccaaactata
    16801 gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg actatttttc tcaagtttgc
    16861 ataaaggaac ttcatcaaat ttcctagcag attccattgc aggcatccat tcagtattag
    16921 acacaaatta cagtttatct gttgaaagta tcgatgttgt tgatttaaca gaaatcaacc
    16981 tgcaacgcta cgatggactg atcgttatga gtcaaagtga tgatgatcag cctttcatcg
    17041 actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc aatgatccag
    17101 cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta gattacggta
    17161 ttggatgcgg acatacgaag atcgcttata ttggcggaaa gaaaaatttt cgttcgacaa
    17221 aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct gtcaatccag
    17281 cctatttttt aacaggagat tacagcttag aaagcggcct ccaaaacatg gaatacctat
    17341 tatcactttc tgacctgcct actctggttt tttgcggcaa cgacgacata gcgattggtg
    17401 cattgagagc tgtacattta gctggcctcc gtgttcccca gaacatttct ctgatcgggt
    17461 tcgatgattc tccagtcgtc tcctatctca atccgccgct taccaccgta cgtaagcctt
    17521 tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa ggcaggacga
    17581 tcgaacaatc taaaattgag ctctccacct tgttgatcct tcgtgagtcg atcaattact
    17641 tgtaaactac aagtaatttt atataaaaat gttaacgtgt gcataacaca gaaaggaagt
    17701 ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac gaagcacctg taaaagttct
    17761 tcagtttggt gaaggcaatt ttatgcgtgg atttattgat tggcagattc agcagctgaa
    17821 taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa cctttagaac aaggtcttgg
    17881 aaatatgatg aaccagcaag ataatttata tacggtcatt ttacaaggac tcagcaatgg
    17941 cgaaatcgtt gatacttccg agattatcac cgtagtagaa caaatgatca atccttatga
    18001 tgaatgggat gcttatctat ctctagcaga aaatgacgac ttggcctata tcgtctccaa
    18061 tacaacagag gcaggaatcc aattcgtgga gactgataat ttaacagata ctcccccaca
    18121 gagcctcccg ggcaaactga cagccttttt ataccgacgt ttcaaattaa acaaagctgg
    18181 ctttaccatc attccttgcg aactgattga cagaaacggc gaacgtctaa aagaaatcat
    18241 tttacgctac gcagaaaaat gggatttaga acctgctttt gttgagtggt tagaaaaaga
    18301 aaatgttttc tgttgtagtt tagttgatcg catcgtccca ggttatcctc gcgaagatgc
    18361 aggtgttttc aatcaacggc ataactatga agatcaattg atggtcaaag cagaaccatt
    18421 catgctttgg gtgatcgaag ggcctaaaga attggagaat caactaccat tcaaacaggc
    18481 tggcttgaat gtcatcatca cagatgatat gactccttat cgtgaacgta aggtgcattt
    18541 gcttaatgga ccgcatacag cgatggtccc tcttgctttg ttggcggaat tggaaactgt
    18601 agaagaagta atgaaagatc ctttattctc cgcatatgtt gatcagttat tcaatctaga
    18661 attgattcct atgctttctt tacctaaaga tgaattagcc atttatgctg accaaatcaa
    18721 agaacgtttc ttgaatcctt ttgcccatca caaattagaa gcgatttcat taaatagtgt
    18781 ttcaaagttc agcacccgtc tgcttccagt gttcaagaag tatatcgaag aacaaaatca
    18841 ggtaccaccg ttaatcactg tttcactagc tgcgctactt cttatgtatc gaggcgatca
    18901 agtaaagcca catgatgatg aaaagacgat aagcgaattt actgatgctt ggtctgataa
    18961 tggaacagct attcctcgat tattgcaaaa cgctgcgctt tggggagaag atttgtctca
    19021 aataccgaat gtgacagata cagttcaaga aatagctgca aagatcgatc aaaaaggtgt
    19081 ccggtcagta ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc ctgcaaccaa
    19141 caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt ccaaaacaaa
    19201 cacaactgac gattgatcag caaacgatca ccactcttga agatatcccg caaggtcata
    19261 agattgcatt gggtgatttg aaagctggag acaacgtgat caaatatggc tatccaatcg
    19321 gtcatgtgac gacagacgta actgccggtc aatggctgca cactcataat gtaaaaacca
    19381 atctttctgg tgaattggat tatcgatatg aaaaagatgt ccatccaagt cattaccttt
    19441 ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg attcgtaatg
    19501 atttgttttt agtacctacc gttgggtgtg tgaatggcat tgctgaactg atcgtcaaac
    19561 agttcaagga aaaacatcca gatctgggac agtttgacca tattacgatt ttaaagcatc
    19621 cttatggctg ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt ttagcggacg
    19681 cagtaaatca tcccaatgcc ggaggagttt tagtatttgg attaggttgt gagaacaata
    19741 cagttcctga attcaaaaaa attttaggtg aatatgatga agaacgagtg aaatttctag
    19801 tggctcaaga tgtttacgat gagatcgaac aaggtgtggc tctattagaa gagcttttaa
    19861 cagcggcgga aaatgaccag cgcatatcag tcccgctctc taagttgaat gtcggtttga
    19921 aatgcggcgg ttctgatggt ctatcgggaa taactgctaa tcctttactt ggtgcatttt
    19981 ctgactactt gattgctcaa ggaggcagca cgatcttaac agaagtgcca gaaatgtttg
    20041 gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga agtttttgac tcaatcgttg
    20101 acttgatcaa tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt tacgaaaacc
    20161 catctcctgg taataaagca ggtgggatca ctacattaga agataagtct ttaggctgca
    20221 cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca atatggcgaa aaaatcagaa
    20281 aaccagggct tagtttattg caagctcccg ggaatgatct tgtagctgcc tctgctcttg
    20341 cttcatccga ttgccagttg gtcttgttta ccacaggaag agggactccg tttggctcat
    20401 atgtcccaac ggtcaaggta tctacgaata caactctttt tgaacgcaaa ggtcattgga
    20461 tggattttaa tgcaggcgtc ttattagaac aaccaatgga agtaatctta gaatcgtttg
    20521 ttcaaaaaat catcgctgta gctagtggag aagaaacgaa aaatgaacaa aatgacgttc
    20581 gagaaattgc catttttaaa aacggagtaa cactttgatt ttcataaaac agtataaaaa
    20641 gaaagaagga agatttatgt ttttatctga tgatttttta ttaaaagacg attgggcgaa
    20701 aaagctttac cattcctacg caaaaaacat gcccattatt gattatcact gtcacttaga
    20761 tccaaaagaa atttacgaaa acaacaattt cagcaatctg acagaagctt ggttaggagg
    20821 cgatcattac aaatggcgct tgatgcgagc ttgcggcgtg cctgaagaaa aaatcacagg
    20881 aaatgcttct gattttgaaa aatttttagc ttggtgtcaa accgtaccaa aaattatcgg
    20941 aaatccgctt tacagttggt cacatcttga attgaagcgt ttcttcggta ttgacttgtt
    21001 gataaatgaa gaaaatgcag agaaaatttg gacacaagca aatgaaattt tggcagctcc
    21061 tgattttcgc cgtcgggaaa tcgcaaaaaa atcgaatgta gaagtgattt gtacaacaga
    21121 tgatcccgca gacgacttgc actatcatca gttgcttgct catgaagaac cagatttgaa
    21181 ggttctacct tcattccgtc cagataaagc actcaatatt gaaaaagaag gatttaatgc
    21241 atgggtcaca ctactggaag aagcagctga ccaaacgatc accacctatc aagaactgat
    21301 agaagtctta gggcagcgga tagaatactt ccatcaacat ggatgtcgtg tttccgacca
    21361 cgctttagac atcttacgct ataaagaagc ggatgaaaca gtattagaag agatttttca
    21421 aaaagcacga gctaacaaac tattatcttc agatgaaatc gatgcttatc gtacagaaac
    21481 actgatacat ttgacccact tctatcattc tcataattgg acgatgcagc tgcacatcca
    21541 tgcttatcgt aactgtaata cacaaatgtt ggaaaaatta gggcccgata ccggctatga
    21601 tggaatgaat gatctttctt tgactattcc tctgcaaaaa ttattgaacc gtgcagaaga
    21661 aacggatcag ttgccaaaga cgatccttta ttcattgaat ccgaatgact atcctgcgat
    21721 tttagctttg atgggctgtt tccaaaaaga acagaatgga aaactgcagt taggttctgg
    21781 atggtggtac aacgatactc gagctggtat gcgcgatcaa ttgacacaat ttgctgatgg
    21841 tagtgctttg gggaattttg tcggcatgct gacagattca agaagtttcc tgtcctacac
    21901 tagacatgaa tatttccgtc gtgttctttg tgaattttta ggcgaaatgg tcatgcgagg
    21961 agaagcacct gaagatgaac aacttttagg aagtctagtt caagcgatta gttatacaaa
    22021 tgctaaaaat tactttggct tttttgccta attgattaat agtaacagta acgaatcaaa
    22081 aacaggatag tgaaataacg gaatccgttt atccacgttt tagagaagaa gacggaccaa
    22141 atttgctccg tcttcttctc ttgctgttct gcttcattgc ctataaacct ttatgaaaga
    22201 agagataaca tgcctactat ccatattgcc ggtgattcta ctgcggcaga aaaagatgaa
    22261 aacaagcgtc ccgagacagg ttggggagaa aaaatctctg cttatttttc acccgagatc
    22321 cagatcaata accaagcaaa gaatggacgc agcaccaaat catttatcca ggaaggaagg
    22381 ttagcccgtc tagcttcaca gtttcagccg aatgatttct tatttgtaca atttggacat
    22441 aacgaccaga aaatccaaga agaaagaggt acgcttccct tcggtagcta tttaaaaaat
    22501 ctgcagatat tttttgaatg cgcaaaaaaa gcgaacgtcc aaatggtctt actgacatct
    22561 gtgacgcgac gagattactt agaaaacgga acattgaatc cagatatact tggagactat
    22621 ccaaaagcga tgcgtgcatt tgctgagaaa caccatattc ctcttttaga tgtgttctca
    22681 agatcacaag aattgttcca gaccttctca aaagaagaga caaaaaaatt ctacctgcat
    22741 cttatacctg atacttgtaa aaattaccca gaaggactaa aagacaatac ccactttagt
    22801 ccagaaggcg ctgataaagt tgcccgaata attgttgaat tgataaaaga atcccgtctg
    22861 cctcttgcta attatttaca aaaaggagtt tgagatgttt ccattaaatt taggcatccg
    22921 tgcccacgat ttagatgttc gaaatcgtga agaaatcgtt caaaaagtat cacagcttca
    22981 cttgtcccat atccaatttg caccacaaaa agcatttcct gaacaactta aattaactga
    23041 catgacactt gggagtgctg cctattttgg tgaatacttc caaaaaaaca atatcgaact
    23101 ttccatttta ggatgctata tcaacctatc cagcaaaaat caagaagttc gtgcacaagc
    23161 attggcaact ttcaaacagc aaattgctct gtgctctgcc tatcaagcca agatgattgc
    23221 tacggaaacc ggtagtgtct ctgtcggtta cacaaaagaa aattttactg aagaagctta
    23281 tcagatcgcc agaaattcta tcattgaatt agtcgctcat gcagaaaatt ttggcgtaac
    23341 tgttgctgtt gaagcaggga tcaatcatcc aatctataac tatcagttag ctaaacgttt
    23401 aatcgatgaa gtagcttcac caaatttaaa aatcatttta gattgtgcga atttgatgca
    23461 taaagaaaat catgctgatc aggaaaaagt gatccgaggt gccttggaca atttacatgg
    23521 ttatattaca gcgcttcatc tcaaagacta tatcatggaa aatggaaaga tccgcatggt
    23581 acctgttggc aagggctgtt tagatttccg cccggtactg cattatatca aatatgagcg
    23641 tcctctaatg tacgccactc ttgaagctac tcctgagatt tatgttcctg aagcaataga
    23701 acacttgcag tttatttacc aagattgtta aggagaagat tatggaggaa cgaatacaga
    23761 aaagttatca aaaatatcat gcaaatgctt ttcagacatt gctgcatctt tatcagggaa
    23821 attattggcg ttttgccatg tcaggactct tttttattat caaacatctt ccatcttggt
    23881 taatgccgat cgctattgcc aatgtaatca atgcagcttc atcgaaagat tcttctcagt
    23941 tgcaggccat ttatttcaac gcttttttga tggcttgtct gattggtcaa aatatattga
    24001 cgaattattt tcatgtaaaa taccatagca cttcgatccg tcaagtagaa gctggactaa
    24061 gagcgaccct tatcaagaaa atccaagaac tatctattcc ttatcaaaaa gaattgcaat
    24121 ctggacgaat ccagtcaaag atcatccgtg atgtagaagc tatccaaacg ttgtcctctc
    24181 aagtatttgt cagcggatta aatatcgctg tcaatttatt cgtagcttta ggtatcacgc
    24241 tttataaaag tcctgttgtt ttcatgtttt tcttattgac tgttcctctc gccgcattcg
    24301 ttactctctt tttccgtaaa aggatcagac agtccaactc agacttcagg caagaaatgg
    24361 aagagacttc tgcaaaagtg atcgaagtga tcgaattagc accgattgct cgagctcatg
    24421 ggttagaaaa acacgaaaca actcgcttaa ataagcattt tactcaaatc tatgaaaaag
    24481 gattccactt agatatcctg caatctgttt tcggatcagt cagctgggct agttttcaga
    24541 tatttcagct gatttgtttg attttcactg ggatattagc tttacaaggg cgcatccaac
    24601 ccggcgatgt cgtgatgtat cagacgtatt tcacgacgat cttcaattcc gtttctggaa
    24661 tgatcacgct gattcctacg atttcaaaag gcatggaatc tatcacttct attggcgaac
    24721 tgttgattgc tggcgatgta gaaacttatc acggtaagaa aaaaatccaa catttggatg
    24781 gaaattttcg ttttgaggat gtttcttttc tttatccgga ctcactcaaa ccgattatcg
    24841 atcatttttc tctagaggtg aaagctggag aaacaattgc ttttgttggg ccatctggct
    24901 ctggaaaatc tacactcctt aacttattga ttggatttat ccagccgact tcaggaaaaa
    24961 tttttctaga tgatcagcct ttggatgaac tggatatgcg aagtgtgcga atttatttag
    25021 ccgttgttcc tcaaaccact ttgttgttct ccgcttcgat caaagagaac atcacttatg
    25081 gcttgaaaaa cgtatcaaaa gaacgtttag atgaagtcat cgaagctgcg caactttctt
    25141 cactgattga ccagcttcct gatggtctgg ataccttagt aggcgaacat ggcaacaagc
    25201 tgtctggtgg ccaaaagcaa cgtatctcca ttgcacgagc gttgatacgt cagccaaaaa
    25261 tcattttgct agatgaagca acctcagctt tagataacca gtcagagaaa aaaatccagc
    25321 aagccctgaa ttacctgaca gaaacaccca ctacttttat tgtggctcat cggctttcaa
    25381 cgatcaaaga ggcagataaa atcgttgtga tcgatgaagg aaaaatcgtt gaaataggta
    25441 cttacgaaaa actgttagct caaaaaggct atttctaccg cttgaaaaat gaatgacaaa
    25501 aaataggagc tatgacaaga cttttgtcgt agctcctatt tctgaataaa cggtgttctc
    25561 ctgccaactc ctcggtcaca agtcacaata gtcatcaaac atgagaagct gttttttgaa
    25621 attgttcctt gtaactcaga gttaaacggt aggctcacga cctcttggta aatggtgttc
    25681 aagagacagc ctatcggcaa taagaagcaa ttttgtcatc gctcaaggcc gaacgtctct
    25741 ttcacaccct ctgttgtttc ataaaatagt tttattcgct gtaaaggtcg cgaactggtg
    25801 tcatgatgat acggttcaag tcattgatga ttgtactgaa gccttgctct ttttgcatca
    25861 agtcattgat tacttcttct gattggattt tcacagccat ttcttgtgct ttcattgcat
    25921 cttcgtcgct gaactcttgt ccactcattt gtttttcttg taattcgatt tgcatcgctt
    25981 ggaagtcttt gaacagtttg tgtgcttctt cgtttgcttg tactttctcg tacgcttctt
    26041 tcaacgcttt gaattctggt aattcacgga tctcacgttc gatttgattc gcgctatcgt
    26101 agatgtttga catgttatgc cctcttttct tttagtagtt actctctatt gtaacatggg
    26161 gttattcgtt ttcaaagggg ctactctcct tgatatttcc gccagaggtc tttagccgct
    26221 tcaccaattc cctgaacgcc ttctttcacc atatcgccga agtttttcag cccttctttt
    26281 gcttgttcac catattgatt caagttatct tgccaatttc cagactctcc attagaattt
    26341 cctgtgtttc caacttgatc tgcagctacc acttgtcctc ctgtggcata ggcatctgct
    26401 acactaaatc ctgtttttga agtatacgga aggatgcctt ctgccgctga tttgaagact
    26461 ttcccgacaa catttccact tgtcccttcc aaatagtgca gcttgctgga ttcttcgtaa
    26521 cccatccatg tcgaaatcac tacgtctggc gtgtaaccga tcatccattg gtcatttgct
    26581 tttgtggcat cgaaattagt ttctgtggtt cctgttttcc ctgctatcgt ataacctgca
    26641 ggattcgcag cagccgctgt tccatttgaa aatgtaccaa gaagcatgct ggtcatttca
    26701 tcggctgttt cctttgatat gacctgtttt ggttttttgt ttgtgttatc gacgatcacc
    26761 gctccagtcg aatcaacgat tttcgtgata agatgcggcg tatacatctg accatcattg
    26821 gcaaatgcac tgtaagcacc cgccatgatc aatggagata ctcctttttc cagtccacca
    26881 agggctaagc cataatactt atcagatttt gctaaaggca ggccgaattc ttctgcttta
    26941 tcatatcctt tttgtaaacc gatctcatgc aaaagccata cagtaggcaa attcaaactt
    27001 tgtgcaacag cttgatacat cggtacttct ccttggtatg tcccgtcaaa attatgcgca
    27061 tcgtaataac tttgcggttc gtctttcaag atactggaag gcttgtatcc agcttccagt
    27121 gctggtgtat atacgcttaa tggtttgatc gttgatcctg gtgaacgacg catctgtgta
    27181 gcaaagctga atcctctaaa gacatgatct cctcttcgtc ctactagtgc ttgtacacca
    27241 cctgtattcg gatctaaagc tactgaagca gattggacca tggcaccatc tgaagcattt
    27301 gctgggaata atgcactgtt ttgataagta gcttctaatt gcttttgata atcctgatct
    27361 aaagctgtat agattttata gcctttattc atgacatctt cttcatcaag accatactcg
    27421 ttaactgctt catcgatcac tgcatcaaaa taataaggat aacgataacc agaatctgaa
    27481 ttatcgtatg tgtcattaag atacgcgctg atatctgtct ggctttctgc ttgttcttgt
    27541 tcttttgtga tttttccgtt ttcttccatt acacttagta ctgtgttttt ccgatcattc
    27601 gcattttttg ggtgatcgat cggattataa ataccaggac ctttcagcat tcctgccaaa
    27661 gtagcagcct cagacagcgt aacattcgtt gcatctactc caaaatactt tctggaagcg
    27721 tcttggacac cccatacccc gttgccaaaa taggcattgt taagatacat ggtcaagatt
    27781 tcttctttgc tgtacttctt ttcgatctcg attgccaaaa ataattcctt cgcttttcga
    27841 tcaaacgttt ggtctaatgt cagatacgca tttttggcaa gctgctgagt gatcgtactt
    27901 cctccgcccc ctgatgttcc gccagacaag accattcgga ctgctgcacg ggcaatccct
    27961 ttgatatcaa acccgtgatg ctcgtaaaaa ttcctatctt ctgtcgagat tactgcatct
    28021 tggatatagg gagaaatcgc gtttaattct acatatgttc ctttttgggc gtacagtgtg
    28081 ccagcttctt cgtttttctt atcataaatg actgtcgatt ggcttaatcc tgattttaac
    28141 gtttcgacat ttgcttgttt tgctaaaata aagaggtaga tacttgtcac taagacacag
    28201 atcaaaccaa tcagcaaaat caatttgttc acatgatatt ttttccagat gcgctttcgc
    28261 catcgatgga attgtccgag atatggtctg atccaacgcc aaaaactgat caaagcagct
    28321 tttatctttt ctaaaatgct ttgaaaatcc atttgcttct tctcctgtct atttgattca
    28381 atcgtcttat tttaccatag tgaaagagtg ttttcttcct gcttaagttg tagttcaata
    28441 tgttttttta cgttagtgca agtagagtaa cataagaatg aacagaaaaa tgtaagtttt
    28501 ttcttaaata tgttttaaaa atggtacaat tagtctgcga ttgcactgaa aggagtacac
    28561 ttgtgaaaat cacgatcact ttacctatga accatcctga aacaacaatt cgcgacttgc
    28621 ttgaaaagga atggttagtt cctagaaagg ttcgacactt tttgcgtata cgtaaaaacg
    28681 tttggatcaa tcaacaacct gctttgttcc atcatgaagt aaagggcaat gaccagatca
    28741 cattgttatt cgaagaaaca gattataatt atcaagaagt tcaattagga gatgcgaagc
    28801 acatccgtgt tttatatgaa gatgagcatt tgattatagt ggataagcct gcaggaatga
    28861 agactcatcc aaatgaaccg acagaaaaca gtaccttact caatcattta gctgcttatc
    28921 tcgacactaa acagcaacga ccttacgttg tccaccgctt ggacaaagaa acaagcggtg
    28981 ccgttttatt tgcaaaaaat ccatttgtct tgccgatact tggaagaatg ttggaacaaa
    29041 aaatgatcta tcggagatat caagccatcg tatggggaga aattgccaaa gatatgacca
    29101 tcaccaataa aattggacga gatcgccatg atcggagaaa aagggtcgta gatgataaaa
    29161 atggtcagag cgccttgaca catgtcactg tcaacaaagt actttctgaa caaacacaag
    29221 tctattgtgt tttggaaacc ggacgtacac accagatcag agttcattta gctcataagg
    29281 gacatccgat cgttggagac cctctctacc aaaatagaac agctgaacga ctgatgctcc
    29341 atgcattaga actgcacatg atccatccat ttactcaaga aaaaatagtc ggtaaagctt
    29401 tacctggatt atgggaatag aaataaaaaa gaacagagtc ttatcagcag ataatgataa
    29461 gactctgttc tttttgtgta atttgtctgc ttacttttga tgttcgtgtt ctgctaacag
    29521 caaactaccg ataatcccac tttcattcgg cagtccccaa cgaacgatat attcacttac
    29581 ttgaggtgtt tccatataac cacccatcaa ttcaacaaat tgctgacgga ttttttgaag
    29641 gagatgatcc tgattcatta ccccaccgcc taaaataatc ttttcaggtg ctaacgtcaa
    29701 cgtatagttg atcagtgctt gagcaatata ataagcctga atagcccaaa caggatgatc
    29761 ttgcggcaga tcttgccctt tgatccctgt gcgtgcctca atagacggtc ctgctgctaa
    29821 tccttctaaa caatctttat gataaggaca agtaccttca taatggtcat ctggatggcg
    29881 tttcacccaa atatgtccca tttcaggatg tgcgatccct gaaaaaattt cttcattgat
    29941 cacgacacct gcaccgattc ctgttcccac ggtaaggtaa atacaactat tttttccttg
    30001 tgctgctcct tgcttcaatt ccccatatgc agcagcattg acatccgttg tccaagcagc
    30061 aggaatattg tagcgttcct tgattgtgcc aagaaaatcg aattgtgccc accctttttt
    30121 gggagttgcc aaaacgtatc cgtaattatc ttttgttttg tcgataccaa tcgggccgaa
    30181 tgagccaatc ccgattccct tcaattcata acgatcaaaa aattcaaata cctgatccaa
    30241 tgtttcttct ggtgttgtgg tagggatact taatttttct aaaatttctc caccatctga
    30301 aaccgcacac acaaattttg ttccgcctgc ttctattccg ccatacattc gttttcctcc
    30361 tcgaatccga ttgtccgaat ctcataaggt tggattattg ggacaaccgc ttgcttcgtt
    30421 ggttcttcaa gcagattcaa tatgacagtt tttccattgt ctttagttat ttccaatttt
    30481 tctaaatgac tagacatatt gaaacctctt actactactt tatcactaaa cttgctgcgt
    30541 ttcaaagctg taattgcaaa cgtttcactt tttatcgtta aatattgttg ttttgaaatc
    30601 aatgttcctt catgatgttt gatttgttga gtggaaaaag gaatctgtgc tgcgtatgca
    30661 tgcaaataag tagagaattt ttcttctggt ccatgtagtt caatgctgta attgaaccga
    30721 tgctcaccta gacactgtgc ttctggtgtt ggaaaatatc cccaatcgcc tagttctccc
    30781 acactgcgaa gtagcgtcac tgcgatttgt ccgtcttctg ttacttcata ttcattcaaa
    30841 ccaaagtttc cgactgttac accgtactct tcatcatgaa tattgacaaa tgcttgctga
    30901 tgttgcggat tcgtggggtt ttcccaactt ttagacacat ggtttgggcg cttcaccact
    30961 tcaaaaatac tatctgcttc gtgatgttcc acatgtaact ttgtaggaaa caagacacgt
    31021 aagcgatgat ctttcatctg attgtcgaga gtcgtttcaa agtcgatttt tttactatct
    31081 tttctgattg tcatgattgt ttttatttga aggattcgtt tttctttgga tcgttctgct
    31141 tttcgataac gaaattccac aaccatctgc tgttcttttt ctaaacgctc gtctgcagaa
    31201 acagggattt ccatttcctg tagaatactt atttttgcta tttccggtgt atctactaat
    31261 acttttactt cagagttttc tttgttactg gataggattg gctgatcaca gaaaggttgt
    31321 ttaaagatat actcatttcc tatatcgccg atatcttcaa aaatatgcag attattcaac
    31381 tttcgtttgt ttttgcgatc ttccattgta atggtaccat ttttttcaat cgtcaaatgt
    31441 agagggccgt tttcgatgat tcgtccactc tgatgaatca ttgtttcctt ttcttctgct
    31501 ttcgcttccc cttctacaag cgcgaatgtt tcccaagaaa atgctggcat gttttctaat
    31561 ggtaaagtca ctttgatcat tcttgccata tatggcacac ggaaacggtc tttcggcaaa
    31621 tcataaccaa attggacagt ttcttcagat aaaatagctg gtatagtggc cccacttttt
    31681 gtttctacat gatacttacg tttaggcaaa ttttctaact cttgatatag ttgttcaggt
    31741 attccttcag cgaatctttt tctttctaat tcgatggtta tttctgcttc accagtttta
    31801 tccattcctg cagtatttac aatgacaaat ggaaagctgt cttttgggaa tccggtcgta
    31861 tcgattgctg cggtcagctg ttctaaggaa tcttgtgcaa gatactttcc aacttcatct
    31921 gctttttcaa aacgaggtat catttcccga tggacagaat cgactgaaca accgcaaata
    31981 ctgtcgtgag gatgattttg cattaatgtc ttccaagcat aatctaattg atcatgggga
    32041 tagtttccac taacttcata agccatcgtc gcaagtggtt ctgtaatatt ttctagctgt
    32101 cgctggacct tggtattcca ttgttttaag taaacccgag cagatgctgt attggctaaa
    32161 gtataccagc catccgtttc ttgacttgtc aattctcctt ctactgagcc taagtcttct
    32221 ggcacatctt tttgaactgc ttccagatag tcagtaaaat tagaatgaat aaactcgtaa
    32281 tctggaaaca gttcatttgc gagatggatt gcttttgata tatctttttg taccggttga
    32341 tgatcaacac cattcatcat caataagtgg ttcgtagagg catattgttc tgcatccgct
    32401 aatttttgct tccaaaaggc aagagctgct tctttttcta ctggaatctc attcccgttg
    32461 ctataccaat tggcaaatag cagaccaaag attgctgttt gatcaggtcc tttccaccac
    32521 atctctgagt attgggaaga atagttttct gcttccaaaa cttgattatc aaagccaatt
    32581 ggttttacac cacggccaaa cgctgctgct gaaataccag cttgtttcat taattgaggc
    32641 gtctgtccca tatttccaaa tgtatcggga aaatagccta acatgacagg ggttccccat
    32701 ttacggcttt cttccatgcc aatcagcata tttctcacgt tcgattccga gctgataaga
    32761 aaatcatctt gcaagatata aaatggtcca attctcaatt ttccttcatt gattgcttgt
    32821 tggattgcct gtcttttttc tggccttact tgaagatagt catctaaaat aatcgtttgc
    32881 ccatccaaat gaaaactgtt gaaggacgga tcaacttcaa atagctctag tagatcgtct
    32941 atcaattcaa tcagtcgcat gtgatgctgt tcgtaagcca tgtaccattc acgatcccag
    33001 tggctatgag aaatgatata tactttcttt ttcatttacg atacctccta cttttctact
    33061 ctaatatcaa agtaatccat gactaattcg caaaacatca tatttgccca agaaaaccat
    33121 tctctcgtat aattttcagg attgttcaca tcgaatcctt cgtgcataag gtgtgttcct
    33181 gcgtcagttg ctacaagtaa attcaatatc cgctcttttt cagatttgtc tgacgttgtc
    33241 attccttcca tagctaatgc aatcggccaa acgtattttt ctggcgtatg ggaggatcca
    33301 attccttttg cgtactttcc ttcatagaaa tatggatttt ctttactcaa caatgttctt
    33361 cttgtttgca gataatcttc ctcttgttca gaacaatatc ccaagtaagg tgcggcgatc
    33421 agattgggca cattactgtc atccatgact gtggcgtttc ccattccatc tacttcatat
    33481 gcataaatcg tttgcccttt ttgattaacc gttcgtccgt gattctgaat cccctcttgg
    33541 atctctttct tcaatttctg tgtttgctgt acaatttctt tatcatcgag tatcgtttga
    33601 aaaatttctt ccacataacc taacacgacc accgcaaaca tattggatgg taccagatat
    33661 ccataacggc aagtatcatc gctaggacgg aaaccagacc atgtcattcc tgtaaaagct
    33721 gtttttgcac ctcgcccgtc attgatcagc gtatcttctt ttcttgtcgt gtctcgtaca
    33781 aatgtataag gagagtgttc atgatcttgc tctgttttga acacgtccaa gatttttttg
    33841 ataccttcaa taaaagattg attgaattga tcagtttttc ctgtattttt gtaaagaaga
    33901 taggccaact gtattgggta gcataaagaa tcaatttcat attttctttc ccagatccaa
    33961 tcgttcattt ttgtatgatc tgtttggtgg ccagcaccgt tagcttcttc gttgaaggca
    34021 ttcgcatatg gatcgatatt gatataaaaa aattgctttt tgactaaccc gctgatcatg
    34081 tcagctaatt cctcatcttc ttttgcaatc actaaataag gacgaacttg tgcagtagag
    34141 tctcttaacc acatcgctgg aatgtctccc gtcaataaaa aggtggttcc gtcattttgc
    34201 tttttgactg ttgtcaacag agtattggca aatgctgctg taaaattctt tgcccagtcc
    34261 gcatggatat ccccacattt ttgtgtgata tcttccataa atttctgcac cgattttggt
    34321 atatctttat aagccactaa taaaaccttc tttctatttg atatatcttt aaagattata
    34381 cttcatacag ccaattatgt ctacgctttc aaaaaatatc tttttattta ctttttcatc
    34441 cattatggta ttatttgaat atgatatatc attaagaggt gtttatatga atcaaccatt
    34501 gtaccagaca atatttaatg atttgcaaac ggctatttta actggtgaat tacctgtaga
    34561 cagccaacta ccaactgaaa aagaactttc acgtacctat aaagtaagca gaatcacttc
    34621 caaacgtgca ttgactgaat tagagcaatt aggacttatt tatcgcgttc gcgggaaagg
    34681 cagtttcgtt aaatccgctt ctccggaaca gattcttgct tctcctacaa aaacacgacg
    34741 gattctgttt cttcttccat atgtcgctga tttgtctgtt ggtgatttta gcaagggatt
    34801 gaaccctgtg atgcaagata aacagattga tgtcatgatg acgacgcttg actttctaca
    34861 aaataaaaca gcaaaagagc taatcaatga atttgacgga ttgatttatt atgctcttga
    34921 tacagaacaa catcttgact tactttttga gctttcattg aaagaattcc ctgtcgtcat
    34981 attagataaa aaaatctatg agttaccttt tcctaccgtt ctttccgata atttccaagg
    35041 cggtgcgctt gctgcctctt atttgatcga acagggacat tcaaaaatcg cttatttgtt
    35101 tggtacgcag actcatccac aatccgttcg acagcgttac cttggttacc ttgaagcatt
    35161 ggacaaagct ggtttgactt tccacacttc actggatgac aaaagcgcga cattagatga
    35221 attgctttct tatatcacta agtatgaagt aactgctttt gtttgtgaaa acgatgttgt
    35281 cgctatccag accatgaatc tcttgagaca gcatggttat catatccctg aagatttttc
    35341 aattatcgga tttgatgata tccaagcagc agctcttgtt gacccgccac tgacaaccat
    35401 cgcacaagat tttgaacagt taggtaagct tgcgggagat gctttggtca cttggttaga
    35461 gacaaaagat cgaccatccg atagtaaatt tccagtttca ttcatcaaaa gacaatcaac
    35521 aaaggagaaa taaaatgaat atcggtcaaa tagatactag acatgcaaca gcgaatcagc
    35581 caaatttctc taatgggaac tgccagcctt atactggcgt tccttttggg atgaattatt
    35641 ttgcaccgca aactacggat caaaacggca gctggtggtt tcatcctgat gaccgcgttt
    35701 ttcaaggcta ccgtcttacc catcaaccaa gtccttggat gggtgatttc agtcatatgc
    35761 tgtttacgcc gatcaatgga aaactacagg agaacaccct tttccatgct caaagttctt
    35821 accgcccaga agaaagtata ttttgcccga ctcatctgtc gatctgtcaa ttacgttatg
    35881 gcatctgttc tacattgata cctagcatgt atggcggaat cttatctatt gattacagca
    35941 aaaatgacag cggtttactt ttatcctttc cgggacgcca tcagctcttt gttatagatc
    36001 cttacacagt ggaaggaaaa atcacacatt ttgctggttc agaagatcct gatttttctt
    36061 tttattttat attgagattt gaatatcctt taacaattga tgagttagct gtttccaaca
    36121 aaaatagcga ctcgatcccg ttgtattttg aacagacaaa aaaacaaact atccgatttg
    36181 ggacatcttt tatcagctcg gaacaagcag cttataacct ggaactagaa caaaaccaaa
    36241 gcaaagagac ttaccttgct cacagtcaag aacaatggca gtcctatttt gatcgtatac
    36301 agctcacaca ccatgatcaa caacaattgc ggacgtttta tcataattta tatcgggtgt
    36361 ttttatttcc acaaacattt tatgaaattg atcctagtgg gcaaaaaatc cactatgata
    36421 cgactagtcg atctataaaa cctggtgttc tctatacaaa taatgggttt tgggatacgt
    36481 ataaaacggt ttatcctctt tattctttga ttgctgtaga aaaatacgaa gaaatgctgg
    36541 aaggtttttt gaacagctat agagaaagtg gctatttgcc aaaatggctg tctcctgatg
    36601 aacgtggact tatgcctggg acattgatcg atgcggtcat tgcagatgct gctgtaaaag
    36661 atatccgacg agacttgatg cctgaatttc taacggcaat ggaaaaaggt gctacggttc
    36721 aaagcaaaca tccaaattat ggacgccaag gaacagaaga ttacttgaca tatggctatg
    36781 ttcctaatca ctaccacgaa tcagtcaatc acacattaga ctatgcttat agcgattttt
    36841 gtattagcca agttgccaag gtactagaag aagaggaaat gacgaagaaa tatcagcagc
    36901 aatcattgaa ctatcaaaat atctttgact atgagtcagg atttatgcgt tcaaaagata
    36961 aagatggcaa gttcagagaa ccattttcag ctaatcgttg gggacaagat tatgcagaag
    37021 gaagtgcttg gcaaagcagc tttgctgttt atcacgattt ttcaggactg atcggaaaat
    37081 atggtggcag tgagagatat aaagaaaaac tcattgagct gtgcaatcag cccccaacgt
    37141 ttgacccagg aggatatggt tttgagatcc acgaaatgag tgagatggcc gcgatagaat
    37201 ttggacaatt agctatttca aaccagccaa gttttcacta tccttacttg ttcagttata
    37261 taggacaacc tgaaatggca cagccactgc ttaaacagtt gatgactcag accttcgacg
    37321 attctccaat gggttaccct ggagatgagg acaatggaag tatggccgct tggtatatct
    37381 tcaatagtct gggattttat cctgtcacgc ctggaaccgg tgaatatgtt atcggtatgc
    37441 cattaattga caaggctgtt ttgcttttgt caaatggaaa ggaattgacg attgaagcaa
    37501 cgcctaatcg gccgcagcaa caatttatcc ataaaatcaa acggatggac aaagaatata
    37561 cgaaattata ttttacccat caagatttgc tgaaaggcgg cacgatctct taccaattag
    37621 ggatcatccc tcaccctaag aaatatcaag caaaagattt gcctttttcc ttatcaaact
    37681 ataattaaca agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaagca
    37741 aaaatagatc ctcatatttt tcgtttggtt ggtcttatta ccgcagatgc aagcttttga
    37801 acaccgttta ttgagaggt
//
LOCUS       DACIRY010000013        35854 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.013, whole genome shotgun sequence.
ACCESSION   DACIRY010000013 DACIRY010000000
VERSION     DACIRY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.013"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(251..1462)
                     /locus_tag="IYE22_000530"
     CDS             complement(251..1462)
                     /locus_tag="IYE22_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9505765.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(1580..2215)
                     /locus_tag="IYE22_000531"
     CDS             complement(1580..2215)
                     /locus_tag="IYE22_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ9505766.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2298..2720)
                     /locus_tag="IYE22_000532"
     CDS             complement(2298..2720)
                     /locus_tag="IYE22_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ9505767.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2738..3058)
                     /locus_tag="IYE22_000533"
     CDS             complement(2738..3058)
                     /locus_tag="IYE22_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9505768.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3351..3542
                     /locus_tag="IYE22_000534"
     CDS             3351..3542
                     /locus_tag="IYE22_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505769.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            3556..3807
                     /locus_tag="IYE22_000535"
     CDS             3556..3807
                     /locus_tag="IYE22_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9505770.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(3966..4733)
                     /locus_tag="IYE22_000536"
     CDS             complement(3966..4733)
                     /locus_tag="IYE22_000536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505771.1"
                     /translation="MDLSKHLYPNEILNVSKLVSETIAPSIEIVNRMAPITGQVSEII
                     KKNSMWTSEIFNESMLKIISDQNMRITQAIMPSLNNIQVGLDLPLLNIQKQITDSMSD
                     FSKVTQAYRSIISDLPKYDFRIDVVSQEIEQVFNEEPEETTNKLLFKDISEPTNELES
                     KLFDILNENREILNYNAEILKEINDTKTKNVADKQSNKEHSSPAFYKNKMWYWEQTLA
                     AFISLVVTDIVNVSVGTDPHNTVLLALLLRCFLQFLK"
     gene            4787..5485
                     /locus_tag="IYE22_000537"
     CDS             4787..5485
                     /locus_tag="IYE22_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ9505772.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNIKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            5663..6004
                     /locus_tag="IYE22_000538"
     CDS             5663..6004
                     /locus_tag="IYE22_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505773.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5997..6668
                     /locus_tag="IYE22_000539"
     CDS             5997..6668
                     /locus_tag="IYE22_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ9505774.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            6674..7366
                     /locus_tag="IYE22_000540"
     CDS             6674..7366
                     /locus_tag="IYE22_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505775.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            7374..7889
                     /locus_tag="IYE22_000541"
     CDS             7374..7889
                     /locus_tag="IYE22_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ9505776.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            7994..8824
                     /locus_tag="IYE22_000542"
     CDS             7994..8824
                     /locus_tag="IYE22_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ9505777.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            8841..9692
                     /locus_tag="IYE22_000543"
     CDS             8841..9692
                     /locus_tag="IYE22_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ9505778.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            9689..10048
                     /locus_tag="IYE22_000544"
     CDS             9689..10048
                     /locus_tag="IYE22_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ9505779.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            10063..10224
                     /locus_tag="IYE22_000545"
     CDS             10063..10224
                     /locus_tag="IYE22_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ9505780.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            10221..10526
                     /locus_tag="IYE22_000546"
     CDS             10221..10526
                     /locus_tag="IYE22_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505781.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            10526..10843
                     /locus_tag="IYE22_000547"
     CDS             10526..10843
                     /locus_tag="IYE22_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ9505782.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            10840..11235
                     /locus_tag="IYE22_000548"
     CDS             10840..11235
                     /locus_tag="IYE22_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505783.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            11232..11570
                     /locus_tag="IYE22_000549"
     CDS             11232..11570
                     /locus_tag="IYE22_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505784.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            11567..11935
                     /locus_tag="IYE22_000550"
     CDS             11567..11935
                     /locus_tag="IYE22_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505785.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            11932..12237
                     /locus_tag="IYE22_000551"
     CDS             11932..12237
                     /locus_tag="IYE22_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505786.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            12261..12464
                     /locus_tag="IYE22_000552"
     CDS             12261..12464
                     /locus_tag="IYE22_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ9505787.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            12461..12757
                     /locus_tag="IYE22_000553"
     CDS             12461..12757
                     /locus_tag="IYE22_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505788.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            12833..13249
                     /locus_tag="IYE22_000554"
     CDS             12833..13249
                     /locus_tag="IYE22_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ9505789.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            13584..14087
                     /locus_tag="IYE22_000555"
     CDS             13584..14087
                     /locus_tag="IYE22_000555"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505790.1"
                     /translation="MVGTSCEMVKHIQKNKDKVSYDIETGIVTTEKGTHGFICSSTGY
                     IRFRLAGKVVQAHTFFGVLIFGSDLEGMTINHKNGNKLDNRQENLEIMTLEDNVKHEW
                     QTGLCVAKKPTNRKRVIQKDLQGRKIREFESITEASKFHNVNPQRIRYVCIGRTVQVN
                     GSSFCFA"
     gene            14097..14168
                     /locus_tag="IYE22_000556"
     tRNA            14097..14168
                     /locus_tag="IYE22_000556"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14130..14132,aa:Lys,seq:ttt)
     gene            complement(14275..14619)
                     /locus_tag="IYE22_000557"
     CDS             complement(14275..14619)
                     /locus_tag="IYE22_000557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505791.1"
                     /translation="MDYYDKNGYAFCYIGDNDEIYSWDGKHIGFKNDQEVYLIDGTWV
                     GFSLNNYIFDENGYVVLYNDKAVNAPTPNVPTFKSIPEIPSIHGIPEIAPVHPIASYS
                     WSSKSPLNLFKQ"
     gene            14932..15276
                     /locus_tag="IYE22_000558"
     CDS             14932..15276
                     /locus_tag="IYE22_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ9505792.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            15281..15562
                     /locus_tag="IYE22_000559"
     CDS             15281..15562
                     /locus_tag="IYE22_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505793.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            15665..15979
                     /locus_tag="IYE22_000560"
     CDS             15665..15979
                     /locus_tag="IYE22_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ9505794.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANEPIP
                     EL"
     gene            15957..17648
                     /locus_tag="IYE22_000561"
     CDS             15957..17648
                     /locus_tag="IYE22_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ9505795.1"
                     /translation="MNQFLSYKHIQAWFNAIESGAIVVCKDQLQLKKYLEERVFTRDD
                     IYFDQQMVEDSINIPATYFPFELIPWEKFVQCFIYGCRWKKDRTLVFNRYLTLMGRGN
                     GKTGYASWNNFFLLTAKHGIKNYDIDIFANNEAQAKTSFEDVYRVIKANPSLKKSFYI
                     SKVSIRNLATNSELRYNTANARTKDGKRPGANRFDEIHENEDYATMNVATSGGGKIQD
                     YREFYDTTNGGVRGGPLDDLIEEAQMIFAGELGIDKDGASFSSLFPFICRLDDDSEVD
                     NPDLWEKACPTINYNQDLKRKMFQEYSQMQRNAGLRLTFMTKRMNKPMEDTRFAVASY
                     DDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALRL
                     QNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILKP
                     ALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLYFGDDAIMRWYCMNVYEEHISNG
                     NIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            17668..18846
                     /locus_tag="IYE22_000562"
     CDS             17668..18846
                     /locus_tag="IYE22_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ9505796.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRSQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEAEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            18809..19495
                     /locus_tag="IYE22_000563"
     CDS             18809..19495
                     /locus_tag="IYE22_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ9505797.1"
                     /translation="MTTMKTFLAVKNEGTVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSVGTEAKKEQSVEEILNDVEEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            19495..20655
                     /locus_tag="IYE22_000564"
     CDS             19495..20655
                     /locus_tag="IYE22_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ9505798.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKTAEPITALDAATMGGLMARLSKFNIEGVDDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            20665..21540
                     /locus_tag="IYE22_000565"
     CDS             20665..21540
                     /locus_tag="IYE22_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505799.1"
                     /translation="MKYILCQPAINQFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTADEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            21537..21848
                     /locus_tag="IYE22_000566"
     CDS             21537..21848
                     /locus_tag="IYE22_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505800.1"
                     /translation="MTPEQVTEELLTAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDGNG
                     E"
     gene            21838..22191
                     /locus_tag="IYE22_000567"
     CDS             21838..22191
                     /locus_tag="IYE22_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ9505801.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            22181..22582
                     /locus_tag="IYE22_000568"
     CDS             22181..22582
                     /locus_tag="IYE22_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505802.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFKLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQTLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            22575..22979
                     /locus_tag="IYE22_000569"
     CDS             22575..22979
                     /locus_tag="IYE22_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505803.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSDIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            22991..23596
                     /locus_tag="IYE22_000570"
     CDS             22991..23596
                     /locus_tag="IYE22_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ9505804.1"
                     /translation="MAKTTTVVTTFDNVSIKRIAFNFKNAENAIATDCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLRQDERIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENSKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKSSTTTTTTTKA"
     gene            23618..23980
                     /locus_tag="IYE22_000571"
     CDS             23618..23980
                     /locus_tag="IYE22_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505805.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            23983..24165
                     /locus_tag="IYE22_000572"
     CDS             23983..24165
                     /locus_tag="IYE22_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505806.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            24182..27601
                     /locus_tag="IYE22_000573"
     CDS             24182..27601
                     /locus_tag="IYE22_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ9505807.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEFVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGDTWESIKEGASSVWDGVKET
                     WSGFVDWIQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNVWSTVSSWLGGIWNSISSTASNIWNSVTSSINNAINAAK
                     SVIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            27652..28389
                     /locus_tag="IYE22_000574"
     CDS             27652..28389
                     /locus_tag="IYE22_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ9505808.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPRSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            28399..30690
                     /locus_tag="IYE22_000575"
     CDS             28399..30690
                     /locus_tag="IYE22_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505809.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHETRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVEDYPAGYVSFVDFKDKATDVASLREAA
                     KNWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGLDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQDGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYKTSIANGKITWHSKKVDKD
                     IMELEARDDIDADVGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            30714..32852
                     /locus_tag="IYE22_000576"
     CDS             30714..32852
                     /locus_tag="IYE22_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505810.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIEFGGYDYSGNPNFLSKLSYDLVV
                     NQNGSVGTVSQGENSFKYNKTTKDVDGSVALYYKAGGRANWLPSNKRIVMTVKLRAGV
                     GYIPADGRKVLIRYRYIDNGTSKIPVDLQVNSASLTQEWQEFTVTGTTQTFSQQANDP
                     WVQFYVQMGILGEIEMSYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENIANKTIKY
                     PIKTSAYATYTASNIENYQANQTYTLTMKATKPATQTFGVFLKGGARGVGNMVPVEGK
                     TDEWSLSFTVTQAHINAGVTRDLSIYQLPQATIGACQIDWLKIEKGDTRTPNIEQYKY
                     RGIGMRDSNNPKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTAS
                     INEVDTTAKDAQTKANANETAIKSTNSRVNEIENIITALTKEETYLLPASIVSGGSLK
                     FTSRGGMTVATGSFKLAKDIAFGEVLVNDLPDSMINIDNNAIGTGAVTGNSVSQIYVE
                     SNTRKVVANTAMKKGQWFTVGFSYHSKLSI"
     gene            32909..33151
                     /locus_tag="IYE22_000577"
     CDS             32909..33151
                     /locus_tag="IYE22_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ9505811.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            33167..33364
                     /locus_tag="IYE22_000578"
     CDS             33167..33364
                     /locus_tag="IYE22_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ9505812.1"
                     /translation="MILPDKYYQVIKWTVLTVLPATSVLVATLGKAYGWNGTDMTVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            33375..34391
                     /locus_tag="IYE22_000579"
     CDS             33375..34391
                     /locus_tag="IYE22_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ9505813.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGANEGSSQVAN
                     NQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSDDKPVIDPTRAGAANP
                     TLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPQR"
     gene            34576..35559
                     /locus_tag="IYE22_000580"
     CDS             34576..35559
                     /locus_tag="IYE22_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004048082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HAQ9505814.1"
                     /translation="MNEKGKSIDGLMRHMRNNHNVKINGSRDKQNLLNMGYYHGYKAS
                     MYIKERKNMQQFEEFYEVKAVYDFDLEMKTIFYPMLIKIETSLKNRLIDFLVKGEQCD
                     IEYIYKNKLRDYASKEVGSKDYKKYLAKNLKLREKIDETIAYNYGNGHPAVQHFFHCN
                     KPLPLWAYFEIITFGEFGNFIGCLNKELRINFAESINLHHSGFNQNGRVIENIVFCLT
                     GLRNATMHNSVIFDCRFNNSNTSNQLKGYLEKVTGIKNITFDTLMDFMILLVMLLKKI
                     NITKNELNRYVRHFDDIRETLFLSVPFSTYTAIIGTDAKNKIVALKNFIRK"
BASE COUNT        12611 a         5419 c         7360 g        10464 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttatata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta atagtttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ctaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gtaaagttcc gtcttgtttt gtatcaaata
      541 taaaatagtg tcttaatttc tttatattta atttccaacg aattttttta ttctctttga
      601 tcaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttcttccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggttttat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaataaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctattcgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtaccctaat acagctctta gtctatatgt ttcattgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctattgtgta ggtttgtttt ttcttagaac acgctcgctt tggtcggtgg
     1561 ggcgtgtttt ttatactaat tagttatgaa gagcaattga aactgtctct gttccaaagt
     1621 aacctgtttg tgcttgcaat acattatttt gtgcatttgc ttgggcttca gagatatcaa
     1681 aaactatttt accagtttgt tgcatatcag gatttaagct ttccataaag aaagagttag
     1741 tgatattacc attttcatct tgatttgccg acatagatgc agccgaatca gcttcgaaag
     1801 tttttccatc tgcttttaat ttgaagaatg agctatcaac agtcacagcc ttatcacctg
     1861 cattttttac agataagtca actactaaga aagtgtcttt tgcgttagta ggaagtacag
     1921 atgggccaac ttgttttact acttcaacgg agtttacttt gtattccatt ttccctacag
     1981 caacgtcatc accgatttta taagtctttt cttcttttgt tgtagtttct tttgtagttt
     2041 ctttattagt acttgttgat gttgcagtag ctgaatcttt gttttcaccg ccacttagag
     2101 caccaccaat accaaaaaac aaaagtacta ttaataccca aaaccatacc cgtttataaa
     2161 acggtttttt aaccttatac attttcccat cttgacccat aacttttttt gccatttaaa
     2221 tatccctcac ttcttgttat aatatatttg tgatctcaga aatgaggtat gagtccgtgt
     2281 tgcagcacgg gcttttttta ctgtgcataa gagtattttt tcttgaaata tgactggcaa
     2341 acattaaaac attctgttct taacttatta ttgatagagt agaattccat gaaattttct
     2401 aatttgaact gagattcatc agtcaattcg ttctcaataa agatatttag taaaatcata
     2461 attgcgattc tatcagcttc agcttcgaat tttgaatgaa aagttgtaga gttatcgtac
     2521 agtactgaat attcaaaatg tgaagcaatg aaatgaccga gctcatgggc taaatgaaaa
     2581 gcttcagaac tgtcttcgtg tagtttttca ttcaaaaata ctattcttgg ttttggataa
     2641 taaaaacctg gttcttccat ttccatatag attaacttta aattatactc actcagcatt
     2701 tctttcaact ttaaatacat acaacccatc actccaacta ttcattttcc tctaaagctt
     2761 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
     2821 atacagtatc atcttctgat aaatccacat gtttaggcgt ttctttttct tctctaccta
     2881 gaagatagtc tacagagaca ttgaaatagt cagctacctt ttgcaaatct cttgctttgg
     2941 gttctgttgt tttccatcga tagaaaagat tttcgctaaa tcctaattct aaagcgactt
     3001 ctttcatgct tttatctctt tggctagcta atgattttat cctttcaaac agtgtcataa
     3061 taatgttctc caatcctaaa caaataaaag tacacaaaat gtaaattata ctgttgacat
     3121 aagtataagt ttgtattatt atttgtttgt aagttaattt gatagaaaaa aagcaaacta
     3181 aaaatacacc ttatagcatt aagtttggcg accgattgca taataaaggg tttgttgtag
     3241 gcttatttaa ctatgtttat atagtatacg attgtatact attagtcaat agtttatacg
     3301 ttttttctat cattttgact tacaaatcta tatgtaaggg gagtgaatag atgtcaaatc
     3361 tagataacgg ccgtgctgca atcaagaagt ttatgcgaga aaacggagta actatggaag
     3421 atttggctac tgcatatgga tacacacgtg ttcgaatgca gcaaattatt gatggccatt
     3481 ggagtggacc aaaagctaat aaaactatct tagaaatcat ccgtgattac agaattcgat
     3541 aggaggaatt aatcaatgga acaactagct ttagttaacc tctcagccct caaagcttct
     3601 ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc ggaatatttg
     3661 aaaaccacga cacgcacgct aactaaagat gccgaatctg gaaagattcc agcagcgaaa
     3721 gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa aggaggaaaa
     3781 acatgggcaa attcaacaga gcactagtat tcagcgcacc gctaatcatc tacgctttag
     3841 ggctttgggg aaatagacaa gcgttgatag gaacgattgt ttacatggtt tggattttta
     3901 tggggcttga tgaagctgag tacagagcga aaaagccagt cggaagggac tgactaataa
     3961 atctattact tcaaaaattg taagaagcaa cgtaagagca aagccaataa aactgtatta
     4021 tgaggatctg ttccaacaga tacatttact atatcagtta ctactaaact tataaaagca
     4081 gctaaagttt gctcccagta ccacatttta ttcttataga aagcaggtga actatgttct
     4141 ttgttagatt gcttatcggc tacatttttg gtcttggtgt catttatttc tttaagaatc
     4201 tcagcattat agttaaggat ttcccgattt tcattcaaga tatcaaagag tttcgattca
     4261 agttcgttag ttggctcaga tatatcttta aaaagtaatt tattggtagt ttcttctggt
     4321 tcttcattga agacttgttc tatttcttga gacactacgt caattcgaaa atcgtacttg
     4381 ggtaaatcag atatgataga cctgtatgct tgggtaactt tagaaaaatc cgacatacta
     4441 tcagtaattt gtttttgaat attcaacaat ggcaaatcta atcctacttg tatgttgtta
     4501 agagaaggca tgattgcttg agtaatcctc atattttggt cgctaataat ttttaacatc
     4561 gactcgttaa atatctcgga agtccacata gaatttttct taataatctc gctaacttgt
     4621 cctgttatag gagccatcct gttaacgatt tctatagaag gagcaatagt ttcgctgact
     4681 aactttgata cattgagtat ttcgtttgga tatagatgtt tagaaagatc cacttatata
     4741 caccaccagt ttttaactaa ttatatcaaa aaggagagaa gaaataatgc aagaattagt
     4801 aattttgaaa aataaagaag ctgtgactac gagcttacag gtcgcagaaa gctttgagaa
     4861 aaaacatcaa catgttttaa gagatattga tgcgctaaaa aaagatgtgt ccaattttgg
     4921 acagatgttt gtagaaggta atgaaccaga ttcatacggc agaaatcgac gagttttttt
     4981 cattagtaga gatggttttt tcttgttggc tatgggtttt acaggaaaga aagctattta
     5041 cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttattc gcaagaatac
     5101 tgttcctcaa acaattgaag atatgatgat ctaccaacta gaagaaatga aagatgttaa
     5161 aaaagatgtt tccatgctta aagatactat gcgaattagt gggcaacaag agtttgaaat
     5221 taaacaaaaa ggaaatataa aagttatgga agttctaggg ggtaaagaaa gccgagctta
     5281 tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac gtaccttttc
     5341 aatcccaaga tatggcgagt tacctcgtaa gagatttgat gatgctgttt catttattga
     5401 gatgtggtta ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag
     5461 actttttggt gatgaaaatg aatagagctg aagcgttaaa aatcggtaaa ttgattgctg
     5521 ataattggtg ggaaaataac tatcctatta ttttaagtca acaacatatc gataagaaaa
     5581 aagcatggca acaaataaaa aagtgactcc gccggcaagc aaagagtcac aaagaaaaca
     5641 catcataagg agattttagc atatggaaaa agaactttcc actctagatc aatatttgat
     5701 tgatcctgat tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa
     5761 tcttttgacg aatgaagaac tagcttggga tcaagatgat ttaggcaacc atgtaactat
     5821 ttgggatcat gtttatctta tccatctatc gaagcattcg aataaacctg aatatattta
     5881 cgtcatcgaa gatggcttga ttgatgcgct agaagagtac gacagagata acttgattga
     5941 tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag aatttgatga
     6001 gtgaaatcaa agggacaacg aactttgaaa aactttttag tcgtaagtta aataaaattc
     6061 tcaagaaaaa aggaaatttt gattatttat cttgggctca cgcgtgggag attatgaaaa
     6121 agaatgatcc acaggcaacg gtaactatta atgagtacaa acactacagg gttgtttctg
     6181 gaactcatca agactttctt gttgaggaat ataaaccttt tcttatggat gaaactggga
     6241 cttatgtatc tgtctcagta acggttaaag gacacacgga aaccgaatta tttcctgttt
     6301 tagattatcg aaaccaacca gttgttaaac caaatgcaat gcaaatcaat aactcattga
     6361 agcgatgctt tgtgaaagca ttggctctac acggactggg attatatgta tttcaagggg
     6421 aagatattcc aacaccacct agaatcgata caaagaaatt aaacatgcta gagacgattc
     6481 tagaagcttt caatgagcag atgggtaaag atatgaccaa aaccttaatc gaatatgtta
     6541 atgagcagac agataaatta gggctcttag ctgataacgt tgaaactatt gaacagttaa
     6601 gctatgagca atgtgccttg atggagcgag caatagcagc taagagaaaa gaattagata
     6661 agaagtgata tgagtgttta aaccattaat cgattcatat tcagcggttc tgaaaaagtt
     6721 caaaggaaaa gacataagcg caaccatcaa tgaggaagtg aacattgatc gactaaagac
     6781 gatgtatgac ggctacgatg gtgatcgagt cattgaaatt cgttttattg atcctagacg
     6841 tttcactgta cagcaacgaa acttcatcta tgcgctgata ggcgatattt tcatcgatac
     6901 aggcatgcca acggacttct ggaaggaatt cttctacttc cgtttcgaag gtgtcacagg
     6961 gcgcaaaata agcctcaaag atgaatcgag cacaaccgta agtgatgcca atatcttagc
     7021 gaatatcatc ctagatttca tctttgagca tgatattccg tttaaaaatg gatatgaaat
     7081 tttgccccaa aacgaacagt atttctttta caaatgcctc acgaacaggg tgtgctgtag
     7141 ttgcggaaag aaaaatgctg atatacatca cgtagactct gtcggcatgg gaaataaccg
     7201 aaaaaaaata aacaattctg gtaggcgctt tatggctctg tgcagagaat gccatacgaa
     7261 aattcatgtc gaaggtttta ctacatttac aactaaaaga aagcttacgg cagttgttct
     7321 caaagacagt gatttaaaaa gattggggtt gaatatcatt gaatgaatta tggatggata
     7381 tcgaaggata cgagggagta tatcaagtta gtagcttagg aagagttaaa tctaatcgcc
     7441 ctaactatgt aattaataag ggatacgtta acaaagaagg atttattatg aaaccatctg
     7501 acaatgggaa agggtatcaa attatcttct taagcggcaa aggttttaga gatagaaggt
     7561 atgtccatcg gctagtagct tttcactttt taaaagaagc gtatttcgaa ggtgctgaag
     7621 tcaatcataa gaacggagat aaatctaaca atactgttga aaacctagaa tggctcagtt
     7681 ctgatgataa taaaaagcat gctagaaaaa atggattaac taacttgaaa ggaccatcaa
     7741 aactaactga tttgcaagct ttagctatca agcgtttata tacaaacaaa ttgatgagct
     7801 ccggagaaat aatgaaactt tttaatgtca gcaggcatac cgtgctgaat atagcttcag
     7861 ggaagacatt tagtttcttg gaagattaag agaaacacaa aataggtgtg actaatttca
     7921 aaaataaata tcaaatcaaa gggatcaagt taaaccagga aacaattaaa aagttaagga
     7981 taggaggata aatttggctg agataagttg gatcaaactt aaaactacta tgtttgacga
     8041 tgaaaaaata cgattaatcc aagctgttcc tgagtcggat gccatcatcg ttatatggat
     8101 tcgattacta gttttagcag gaaagactaa cgacgatggt ctgatatata tccagaggaa
     8161 catgccttat accgaagaaa tgcttgctac attgtttggc aaaaacgtaa atacggttcg
     8221 cttagcgcta actacattgg caaatttcaa catgattgat ctaagcagtg atggactaat
     8281 tgccatcagt aattgggaaa aacatcaaaa tatcgagggt atggataaag taaggctaaa
     8341 aaatgctgaa agaaaccgta aatacaggga aagaaagaga caggaacgtc tcaaattgga
     8401 aaatgacgtt agcgtgacgt cacgtgacgg tacagataaa gatatagaag aagataaaga
     8461 tatagataaa gaagaaaaga aaggtaagta ttctgacgaa cacttacgcc ttgctaaaaa
     8521 gttgcaaagt aatttaactg aagattttcc aaaagaaatg aacaaagtag atatcgaaaa
     8581 atgggcagac acaatcaggt tgatggaaga aagagataaa gcatctatag aagcgattga
     8641 gtatgtgatc aattggctac ctacaaatga attttggttt ggaaatatta gaagtgctaa
     8701 gaaattgaga gaaaaatttg agaagctcaa attcgaaatc aaagcagaca agaagaatca
     8761 taaaaagcaa agtcaaaaac tacagtacag caatcctagt gaatatgacg acttgccaat
     8821 ttaaaaagga gatgcatcac atggaaagcc tagcaaatgc tatggagaaa ctaataagaa
     8881 gagtattagt gcaaagcgga aaatgtccag aatgtagcga acctttgtat agttggcgag
     8941 ctaaaaataa ggatggttca gaacgttgta aaccaacatg catgagttgt ggttataaag
     9001 cgttacgtgt gaaagaggat atacagaccg aacggatata taacgatagc ttaaaagcac
     9061 gagcgttgag tttttttcaa aatggttcgg tattaacaga taaaactttg tttaaatgca
     9121 aaatggaaaa ttatcacgta gtggatcaag aaacaaaaat cgctttagaa aaagcaaaaa
     9181 gctatacgaa tgaggttctg ctgaaccatc ctgcacattt cattctatca gggaaatcag
     9241 gaagcggaaa aagccactta tcaatggcca cagcttggga aatacttgag cgctcaaatt
     9301 atgacaagaa aatacttttt ataagctatc aagaattatt agagcaaata aagttttctt
     9361 ataacaatac tgaactgaga aaagaaattg aaggatcgct tatagccgat attaaaacaa
     9421 ctgatttggt ggtttttgac gatattggag ctgaattagg tagtggggta tcaaatagta
     9481 ggcagtttac aaacaacacg ttaaacacgc tcttagaagc cagacagaac aaggcaacga
     9541 tcatcacaac aaacttatct ggtcctgaac taagagaagc ctacggtgaa agaattgttt
     9601 ctaggatatt taagaattca gaaggttatg cgctgaaatt ccaacaaaca gcagacaagc
     9661 gcataaaacc agtgaaaggt agtatcgcat gaataaatac cgtaataaaa aaactgttca
     9721 tcgaggtatc aagtttgatt ctatcgcaga agcagagtat tacgatctag ccttgtggca
     9781 agctgaagca aatggctgga aagtaaaact tcaggaaaga tttgagctga tgccgaaatt
     9841 tgaactagac ggaaagaagt atcgcaagat cgagtatatt cccgacttca cattttataa
     9901 aaacggcaaa cttgtcaaag tcgtagatgt caaaggaatg cagacaaaag actttaagat
     9961 caaggcaaag ttgttctgtc atcaatatca agcgccgttg atattagcta aaaaatatcg
    10021 gaatacgttc aaggaagagc gtttttaacg aggtggtcca tcatgacaac agaagaagtg
    10081 attcaaatgc gtattcgaaa cattcagcgt gaaattgacg atctagaacg aacaaaggca
    10141 gtgatggtca atgaaacggc gagaaaggca atcgatttgc acatagagaa cttaagaagg
    10201 gaaattcgta gattggagga atgagcgtgg ataagaaagc gacaatgaaa cgaattgctg
    10261 aattaaccaa gtcagaatct tggcaggaag acaaagaaat agttgcagaa gtccaaaagc
    10321 tcggtaaatc aatgtggact gaaaagccta aacggaaaac gccgagaaaa attgcaatct
    10381 ggcatggtga tcgaattcta gtaacaggta cagctgaaca gttatctgaa attactggat
    10441 taagcaaaaa cattatctgg gatagagcta ggagcttatg gattgattca aaaggacgac
    10501 agtttaggta tgtggaggag agataatgga tctcattaca caatacagtg acatcatcct
    10561 caagaaaatc atgatgaaga ttcagaaaga caaaaaatca aaagaacgag cggaattagt
    10621 taagttggaa atggctgaaa caggagcagg agtgcgaagt agcaggcatt ggaaagcagc
    10681 agcaaacatt gaattttatt acaacgaaat tcaaaaaggg ttcgatcaga tgcgtgagct
    10741 ggatcggcaa acaaattgga gcaagaaact tcatcaagat cgtttcaagt ttgtaaagaa
    10801 atataaagag atattagaca aatacatgga ggacagcaaa tgatactgaa gtacagggca
    10861 tggaataaga aaactcagtc atttattgat tatggtgatt tggttttaga tttgagaagc
    10921 ggaaaaattt acgctgggga cattgggtta gtggaaagca ccattgatgt gactgaccaa
    10981 attgaactca tgcaatccac aggaatgaaa gataagaatg gtgtggagat atttgaaggg
    11041 gatgtagtaa ttacatcaaa aggagcgatt ggatatgttg cgtatcttca acaagaagcc
    11101 ggctttgtag ttgtactaaa gaaaagtgat tatagattag gccataggaa tactggcgaa
    11161 agttatgatg tagcaactgc tcatgaagtc atcggaaata tatacgagaa tagcgagtta
    11221 ttggaggtag agtgattatc ttagctactg actacatcga ttctttaaaa gatgaaaatg
    11281 ggaatgtccc agcattgcta aattgtggaa ttaaaagcaa gaatagaatc attttgttac
    11341 ggatatcggc tgatttagca aaatatcttt atctgagcaa gacagcagta tattacactg
    11401 cggctacacg ttatcagtac aagggaaaaa caatcagtca ggattattat gatcgtttaa
    11461 tcagtcctga catgcatgga aaatcaaaat cagcgatcaa gttatttggc gcaatagaga
    11521 tatatgccga tgattttcca aatttatggt tgaaggagga agcggaatga acgaggaaat
    11581 aaaaaaatct attcgttaca ttgatagagc gttatcgcaa ctcgaaccct tgcgaattga
    11641 aagtcataaa ggaatgagtg tctacccaaa tcagtatgac aaaatgaacg ctgatttacg
    11701 taatgcaaag ctggccttaa ttaacattaa aaataacaaa ggctgccagt attgcaaaca
    11761 cagcgaaccg ttgaatgata ccagcaactc taactttgca actgcaattg agtgtgagga
    11821 aaatggatac tacattgcaa ctgagtatga cgatggcgat tatgcagata atggctattc
    11881 tgcttatatc caatattgcc ctatgtgcgg tcgaaaattg gaggtagcgg aatgaatgtt
    11941 caagtttatt taaaaagaaa tttggccagg tttcctcgaa gctgggatac aaattcccac
    12001 ccatccgagg cagaagaaga gagctatcta atgtctgcta acatgataga aattacaaga
    12061 gatgatgcgg atgagtttga taaaaaaaga tcatggtact cggtttcgaa tccaatgtat
    12121 acggctgtaa tagaagatta tcatatatcc gacagattta ttctggttga tctagaaaga
    12181 cctaagaaac gaattaaaca gtatagacgt tgcgggtttt caacaaagaa atggtgaaaa
    12241 caaaacagag gtgactcaga atgaacaaca gacatcgcag aataacaaaa ctaagaaaac
    12301 aggaactgaa tgtactaaag acaaagtttg aaaaagaata cggagtttct gcagaagaaa
    12361 catataaagt ggcaagtcag tgtgttgctg atgcaagtga tgctattcgt aagtttggaa
    12421 tttcgatatt aagtgatgat cgtaaatggg aggaaaaaga atgaaactaa aagacggatt
    12481 ttacgctagt agccacggta tcggcggttt aatgctggat atgcagacaa agaaccctaa
    12541 aacacgtaag aaaccaaaat tcaaagtcgg tgacatggtt cgctgcgaag cagaagggtt
    12601 catctatcca tttcgtggat atgtagaaca tgtctataat cactcagcga tcattcgcat
    12661 tgaaaacacg atggaatgcg acaagttatt agcgaaaagc aaagagaatt tagcagtggt
    12721 gagattggtg gatattgaac taatcaatga caaataaaaa agccggatcg ctccgactaa
    12781 cataataaaa cagacaagtt tattatatca cataaaggag cggtttgact tgatgcaatt
    12841 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
    12901 tcggcgtttg gagcgtatgg caggtcgctc tttgatagat attaagtcgc cgattataac
    12961 ggatatgccg aaggcgccga agcacggcaa taaggcagag gacgcgatca ttcagatgat
    13021 ggatatagaa gcagagagag atgcgattct agcggctttg atggctctta gtctgattag
    13081 tcgtcagata ctctactaca gcttctgcga cgtaaataag cattctaatt atgaaatagg
    13141 tcaattgata cgaggatacg gagagaagaa tgtagagaag ttgaaatcca tcgcattgat
    13201 tgaatttgct gaagcatata aaaaaggtgt attaattcag tatcgttgat tttgtagggt
    13261 ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatgg tagtgtcgaa
    13321 aattaggaaa caggacttcg acaaaataaa atgtaaggga ggaaatctcc ctcatcgttt
    13381 aattaagctt cgatagacag cagcggaaat attaagaata aggatgtgaa ttttaactcc
    13441 ttctaaattg ttcttattat ctatcatccg ttgctgtcta ttgtcattat gtcactgtgg
    13501 cggaaagggt agacgcatgg atgaagtatc ccacttctga ctgcgttttt tattttggga
    13561 atattgcttg ggaggtaatg aaaatggttg gaactagttg cgagatggtc aagcacattc
    13621 agaaaaacaa agataaagtg agttacgaca ttgaaacagg aattgttacg acagaaaaag
    13681 gtactcatgg gtttatatgt tcatcaaccg gctacattag atttaggttg gcagggaaag
    13741 ttgtacaggc tcatactttt tttggtgttt tgatattcgg atcggatttg gaaggtatga
    13801 ctataaacca taaaaatggt aataaattag acaatcgcca agaaaacttg gaaataatga
    13861 cgctagaaga taacgttaag catgaatggc agacgggatt atgtgttgcg aagaaaccta
    13921 ccaatcgaaa aagagtaatt caaaaggatt tgcaaggaag aaaaataaga gaatttgaga
    13981 gtattactga agccagtaaa tttcacaatg tgaatccaca aagaattaga tatgtttgta
    14041 taggaagaac tgttcaagta aatggttctt ccttttgttt tgcttaaata ttttattgac
    14101 gtgaagcatc tgtggtgata cgtctgactt ttaatcagaa ggtgcaggtt cgaatcctgt
    14161 cacgtcaatt agtaaccgag gctgtggtag ggaggagaaa cggacgtcga gcttttgtag
    14221 gttacttaat atctcgtcat tagttcaaat cgctgaaaca gtgagaaaaa gttctcactg
    14281 tttaaaaagg tttaatggag atttggaaga ccatgagtaa gaagcaattg gatggacggg
    14341 cgctatctct ggtatgccgt gaatactagg aatctcgggt atagatttaa atgttgggac
    14401 attaggcgtt ggagcgttaa cagctttatc gttataaaga actacataac cattttcatc
    14461 aaagatataa ttgtttaatg aaaaacctac ccaagtaccg tctattaggt aaacctcttg
    14521 atcgttttta aaaccaatgt gcttgccatc ccaagaatag atttcgtcat tgtcacctat
    14581 atagcaaaaa gcataaccat ttttatcata atagtccatt ttacatcctc cttttattgt
    14641 tttaatcaat tataacaaat ttatagggta taaatgcaaa taattcaaaa actactcaat
    14701 aagtggtctt tttattttgt acaaaggagg taacaacaat gtatagacca caatacttag
    14761 aacagaagca tgaagtaatc actgtgcaaa atggtaacgg tgagatagta cgaaagtata
    14821 gaagaccaat aaagagcgat acatataaac gaaaggaaaa cgatgaagtt attccattgt
    14881 atggcaaaag aatagctaag cattaaataa gattgcgaaa ggaaatggaa catgaccgag
    14941 gaattctata gatggctatt acagttgata agagaagatc gtttggttaa gttctatcag
    15001 tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac gagatcacta tgaatgccaa
    15061 gagtgtagaa gactaggtaa gtatcataga gtagagaacg ttcatcatat aaaggaagtc
    15121 aaggatagac ctgacttagc tttagattta gataatctta tttgtttatg tgttgaacat
    15181 cataatgaag ttcatggcag atatcttaca gcattggata aacaagagaa gaagatagaa
    15241 agctttgcta acttcgatgc aagtgaaagg tggtaagtgc atgatcatca atgataatgg
    15301 cagagagtat gatacagaaa agattgaaga gtattcatct tatactcagg gactgattaa
    15361 acgtttgata tacgttcgct atgtaggtat tagggatctg ttatcagata actgttgcag
    15421 taaatacaaa gtgaatcaag taagagaagc gttgaataaa gataataacg ttgaaagaat
    15481 aaaaaatgtt tttggatata gcattgaaga gattaattat tacattgact tcgctgaagc
    15541 tttcattccg atggtgagat aaccccccct taaaataaat cgcaaatttt ttgggggtga
    15601 tgaaacggag ggggctagca ggaaaaaaca ttttttcgaa ctttatcatg aaaggagggc
    15661 taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac gccgctcttt
    15721 aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata atgttgatcg
    15781 ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg agaacggcat
    15841 tagattggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg tcaacaaaca
    15901 aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg aagcaaatga
    15961 accaattcct gagttataag catattcaag cgtggttcaa tgctatagaa agcggggcta
    16021 tcgtcgtatg caaggatcaa ttgcaactta aaaagtatct agaagagcga gtattcactc
    16081 gtgatgatat ttattttgat cagcaaatgg ttgaggactc cataaatatt cctgccactt
    16141 attttccatt tgaattaatt ccttgggaaa aattcgttca atgttttatc tatggttgtc
    16201 gttggaagaa agatagaact ttagtattca atagatattt aactctaatg ggtcgaggaa
    16261 atggcaaaac gggttatgca tcatggaaca acttctttct actaactgca aaacatggta
    16321 ttaaaaatta cgatattgat atttttgcga ataatgaagc tcaagccaag acaagctttg
    16381 aagacgttta tcgagtgatt aaagcaaatc caagtctaaa aaaatcattc tatatctcta
    16441 aagtgtccat tcggaatctg gcaacaaaca gtgaactaag atacaacact gccaacgctc
    16501 gaacaaaaga cggtaaaaga ccaggagcga accgctttga cgaaatccat gaaaacgaag
    16561 actacgcaac catgaatgtt gctacttctg gtggtggtaa gattcaggat tatcgtgaat
    16621 tctacgatac tacaaatggt ggagtaagag gtggcccgct tgatgattta attgaagagg
    16681 cacagatgat ctttgctgga gaactaggta tcgacaaaga tggagccagt ttttcaagtt
    16741 tgtttccatt tatttgtcga ctagatgatg acagtgaagt tgataaccct gatctttggg
    16801 aaaaagcctg tccaacaatt aattataacc aagatttgaa acgaaagatg tttcaagaat
    16861 attctcaaat gcagagaaat gcaggactac gtctaacatt catgacaaaa cgaatgaata
    16921 agcccatgga agatacacga tttgctgtag cttcatatga tgatgttctt catacgaaag
    16981 aaaaagagtt tcctgaaaaa atggatgaag tgataggaac agttgatttt gctgatagac
    17041 gagattttgc cagcgttggt ttgttaggaa aatatgataa agatgtgtat tttacacaac
    17101 atacttttat ccacgaatca gctcttcgat tacaaaacat caaacgagag gttatagata
    17161 tttctataga tcaaggtaaa tcacaaatcg ttcatgggaa aaatatagaa gctgattata
    17221 tcgtaggttg gtttcttgaa atgagtaaca aatattatat taaaaaaatt gctatggata
    17281 tgtaccgtgc aaaaatattg aagcccgctt tagaagaagc aggttttact gtggaaattg
    17341 ttcgaagcgg atctgttaca catggtatgt taaaagatct ggttgatgac ctttttatta
    17401 atcaacgttt atattttggc gatgatgcaa ttatgcgttg gtattgcatg aatgtatatg
    17461 aggaacatat ttctaatggg aatatacgtt atgaaaaaat agaacctgaa actagaaaaa
    17521 cggatggctt tttttcattc cttcatggtt tgaatttttt agatgatatt tatgattctg
    17581 ctcctgtaac agtcacaaat agctcagtag aaaatacagg aactggattt actcctctag
    17641 tattctaact tgaaaggagg tgagaaagtg gggatttttc aaaaggcggt aggatacttc
    17701 acaaaaaaag caacagttcc tttagaagaa tacttttgta aattgcaagt tgattttgtg
    17761 tatcgaaaat ttgcgattga aacttgtatt gatttgattg caaatgctat gagcaaagcg
    17821 gaattcaagt catatgaaga tggaaaaaat aaaaagaatg atctttacta taggctgaat
    17881 gtagctccta ataagaaaaa taatgcaaca gaatttagaa aaaaactgat taggagatta
    17941 atattctaca atgaagtatt gatcgtttct ccatctaata attctagcga aatatttatt
    18001 gcggatagtt gggatgtcac agaatatgca ttaaaagatg atgtgttttc tcaagtgcaa
    18061 attaacaaca tagtccttga tagagaattt ctagaaagtg atgttatcta tataaaatac
    18121 gcagatcaac aaattaggca actagtcgat gcgtattatc aagcgtatgg gaaactcatt
    18181 tctagtgcca tgaatgttta caagcgttct aacgctcgta gatacgtact gaaagggaat
    18241 ttattccgat cgcaagacaa tacaacacaa gatcaaatca ataaaatgat gacatcacaa
    18301 tttaaggctt ttatggaagc tgataatgca ggtgcggtat ttcaattaca aaatgagtac
    18361 acattagaag atttcagcgg aaactttcaa agcaattcaa gagatataaa aaacttaata
    18421 gacgacatct ttgagatgac agcagcagcg tttcacgttc cgaaaaacct actaaaggga
    18481 gacatgagtg ggttatcaga tcaagtggac gcttttttaa tgttcgaaat cataccgatt
    18541 gctgaactta ttcaggatgc gtttaacgct agtctctatg aagcagaaga atacttgtca
    18601 gggaattttg tacgtgtgga tacaactatg atcaagatta ctagcttcaa agatttggtt
    18661 gacgctattg atgtaggtat tagaaatggg gtatttacaa tcaacgaagg aagagaacgc
    18721 gttggaaatg atcgctctga taaggcgatg gcagatgaaa tatttataac taaaaacaac
    18781 caacaagtat cgaaaggagg tgaggcgaat gacgacaatg aaaacatttc tagcagtaaa
    18841 gaatgaaggc acagtaccgc aaatttttat tcagggattt attggttcta gttggttctt
    18901 tgaagggaat actgacaagg gaatcaaaaa tattttggat agtctaggtg atcaagaaga
    18961 aattgaagta gtgatcaatt caaatggcgg agacgtattt caagggattg ctattgggaa
    19021 cttacttaag tcaaataaag caaaagttaa cgttgtgatt aacggcttag ccgctagtgc
    19081 tgcttcaatt atcgcaatgg ctggcgatac tataaaaatc tacaacaatg cacaattgat
    19141 gattcaccgc gcttccacat atggagaagg aaatgtcgat gatttccgca cgattgctga
    19201 tcaattggaa tcaattgata aatcggtaaa ggcttcatat aaaacaagat tcaacggtac
    19261 agatgaagca ttgcaagaac ttcttgaaaa agaatcgttt atggatgcag aaacagcttt
    19321 gagttatgga ttggtcgatg aaattatcga tgcagaaaac agcgtaggta ctgaagctaa
    19381 gaaagaacaa agcgttgaag aaattttgaa tgacgttgaa gaaaaaagag cagaaaaaat
    19441 tgctgcattt acagcagcat taaataaaac atttggacaa ggagatgcaa aataatgaca
    19501 gttaaaaatt taaaaggtgt aacagctgca agcgaccaat tgatgaaagc ttttaaagat
    19561 ggtaacgaag aatcttttag cgcagctatg gtaagtttat ctaaggaaat tcaggataaa
    19621 attttagaag aagcaacagc aaaaaatcaa gatcaattag tattaatgaa ccgtggccaa
    19681 cgtgtgttga ctacgcaaga aacaaaattc tataacgaag tagtgaaaaa cgaaggtttt
    19741 gcaggggttg aagaattagt accagctact gtatttgaac gcgtatttga agatctagaa
    19801 caatctcatc cactattgca aaaaattact tttgttaaca caactggtgt aacagaatgg
    19861 atcgtgtcac gtggagtcaa tccagcatgg tggggtaaac tttgcgaagc tgttaaaaaa
    19921 gttttagata atggctttga cgtaattaac atgaagcagt tcaagctatc aggttatatt
    19981 cctgtatgta aggcaatgct tgacttaggt ccagtatggt tagatcgtta tgtccgtact
    20041 gttttagtag aatcattgag aattgcatta gaacaagcaa ttgttgatgg tactggtaaa
    20101 gatatgccag tcggaatgat gcgtgatatg agtaaacaaa ctagcggaga atatgctgaa
    20161 aaaacagcag aacctattac agctttagat gctgcaacta tgggcggttt gatggcacga
    20221 ctatcaaaat tcaatatcga aggtgtagat gatccgattt atcgtaatgt gaatccttct
    20281 gatgtggtcc taattgtgaa tccaactgat tactggtcta aagtattccc agctaagact
    20341 gtactaactg ctaatggaga atatgtacaa gtattgccag taccagtttc agacttgcag
    20401 tcaacagctg tgccagaagg aaaagcagtt attggggtag cttcagatta ctttatgggt
    20461 gtaggatcta cgctgaaaat tgaagcttca gatgaatacc attttgttga agacgaacgc
    20521 atttatctag ctaaacaata tgcaaacggt caacctaaac gtaacgatag tttcattgta
    20581 ttagatatta gcgctttggg aactactact acaactacaa aaccaacaac cacaacaact
    20641 acaacacaag cgtaggtgat cagaatgaag tatattcttt gtcagccggc aatcaatcag
    20701 tttaaatggg agcttgaagt ttgtttaact aatctgaaga aactaggaat caaagatatc
    20761 gtattgcttt tcagcagaca cgatgatcag attcctattt tttttgagaa ggaatatggc
    20821 gttgaagttc atgtgtacga tgatctgcgg gacgacaaag agtatattcc ttcgattaaa
    20881 ccatatttat ggtggaaata tttagaagaa gatcattcgc gtgaggacga ccgatatttc
    20941 tatatcgatt cggatgtcat tttcaataaa agaattaatt tgcgcaaatt gccttctaaa
    21001 gatgatgttt ggtattgtag cgactgctgt agttatctaa gtcttgatta tattagaagc
    21061 tgtgaaaacg gagaaaatat tctaaaagat atggcaaaca ttgtaaatgt tacagtagaa
    21121 tctttggaaa ctataaacac taattcagga ggcgcacagt gggttattaa ccgtcctaaa
    21181 gcgaattatt ggaaaaaggt ttatctagat tctaatcggc tatatcgcta cctcagaggg
    21241 caaaaaacaa atatacaaat ctggacagcc gagatgtggg cacagctttg gaacatgatg
    21301 tatttcaata ttggtcctaa agttcacgag gaattagact tttgttttgc tactgatcca
    21361 atagaaaaag ttaaagaagt aaaaatcttg cacaatgctg gagtaacaac agctgatgaa
    21421 gatttatttt tcaaagggag atacgtgact tccacgcctt ttgatgaaga tttatcattt
    21481 gtaaacaaga aaaaatgctc ttacgcatat gttaaagcaa ttaaggcggt ggttagatga
    21541 cgcctgaaca agtgactgaa gaattgctaa cagctgtgaa ggataatatt tacgttacct
    21601 ggaacgaaga agatgagtca attaaaaaga tgatagctaa aaatgctgtt tatcttcaaa
    21661 gtaaagtgag tacaactctt tctttttctc ctgaaagctt agaatacgga ttgctaatcg
    21721 aaaggtgtag atacgactgg aatcgtgctt tagatgagtt tgaacaaaat tttgctagtg
    21781 agttattagg tttcattcaa cattatgcgc tacaagaata tattgcaggt gatgggaatg
    21841 gcgaataatc gtagactcga agaaacattc aacgatggtt ggttaaagat tttgacgcaa
    21901 actaccaaaa gaaatgaact aggaaaaaag attggtgtag aagatacaga aatcacttct
    21961 ttaaaattta gaaatctttc catgagagat agcgatataa cagctatgga tgcgatggga
    22021 tcgaaattaa ctaagaaagt aaagactcca tttcatccaa tcgccaagaa atttaataaa
    22081 gatcaatatt ttatcgtaat cgatagtatg cgttacaacg ttatctatgc cgattacgat
    22141 aatttttata tctattttta tcttgaaagt gtgggtgaat atggtgattg ataattctaa
    22201 agaaaaagaa cgtttaaata agcaaatttc tgctatcaaa acttccttag aagagcattt
    22261 taagctcaaa ctctttcaag actctgttgg cgaggatgag cttcctgatg attttaatta
    22321 cttcattctc gaaacaggag aaatagaaat gatcactgaa ccaaaatata gcgtgggtca
    22381 aactctatat ctaactttct attcagaaaa tagagaagat ttaacaggag attcactaga
    22441 tattatttca ttgattcaaa atcgttcgat tcgttttcag agaatggatc ccaaccattt
    22501 aaaactagag aaccaagatc gttatatcga tcaattggta tttacattta gacgattatt
    22561 gaagagtgat tgtcatggct aaaaatagtt gggagctaaa aataaatgga catgatgaac
    22621 ttcttgtgcg gatggaacgc tattcaagcg agagcgaacg actgattaac gaagcattga
    22681 aatcaaaagg ttcagatatt gcagtggata ggattacgga aaaaattcct gtttctgaag
    22741 cagatttaag aagaggacac caacacgcaa aaaatagtcg tccactaaag actcaatata
    22801 ttaatttggg tttcatcatt agacctacaa gaaaatttga gtatttaaaa tatcctgatt
    22861 tggggatagg tacttctaaa agaaatcagc cagacgaatt catgagaaga ggattaggtc
    22921 ttgcacttga tccaattaca gaacttctga ttcgtcaatt cgataaatta aataaatagg
    22981 gggaacaaca atggctaaaa caacaactgt agtaacaacg ttcgataacg tgagtatcaa
    23041 acgaattgct tttaatttta agaacgcaga aaatgcaatc gcaacagatt gtaacggaca
    23101 attagatggc gaaacagaaa tgcaaacggt ggttaaaaaa tgtggagcga cagaagtaaa
    23161 atcaaaatct aaaccaatca atatgacggt aacaattact gcgcatgtac caatggaagt
    23221 ttatcgacgt ttcaatggat tgagacaaga tgagcgtatt aaaccaggta tttactctta
    23281 cggtcctgat tccgtaggcg aagatttctc acttgctgca gagatcgtgg atgacttcga
    23341 agaaaatagc aagttagttg gtatgttagc atgcacttcg aatacaggat taacattctc
    23401 tattgaaaat ggtgcggatg aagtagctgc gttagaacta gaaacaaaag ttatgcaaga
    23461 tgaatttggt aaattttatc atgaagcaat tgttgcagaa cttgaagaag acttaacaga
    23521 tcaatggatg acgaatctat ctgctgatgt gattaaaaag agttcaacaa ccactactac
    23581 aacgacaaaa gcttaaatat aaaacggagg tagcaaaatg aacgaagatt actcaaaaat
    23641 tgaactaaac gatggaacaa ttttgaattt agaacctaaa ctgaatatca agaaattatt
    23701 gatgatcaat agagatttta acacagacga gtttgcaaaa atgactgtgg gaaaaggatc
    23761 catggatatt tctgttattc aaggtgcgaa ggctgtgtat attgcttacc gccaagcgaa
    23821 catgactgat tatatttcat tcgatgaatt tatcgataaa tgggattttg atatggctac
    23881 tgccagctat atttatcaat tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga
    23941 atttgaaaaa gcaaataagg aaaaaaagct tcaaaagtaa aaatgccaaa gctcttagtt
    24001 gaaacgtggg tcgatgtcta ttcgatgttg accgacgttt tttctatgcc ttcagatttg
    24061 gttttaagcg atatctgttt agatgacatt ttgcaaatgg cttacaacaa gagtgcttat
    24121 gaaggatgga aaaactatgc aataaaccaa tcccagaaaa actaaagaaa ggaggtaaaa
    24181 aatggctaaa aagagaacag aagcagaagt aactttcata gctaacgatg acggattgaa
    24241 atctacgtta aaagaaatca gtgctgaatt aactaaaaat agagcagaat taaaactaga
    24301 acaagctcaa ttacaacaga ctggttctga atcagacaag ttaggaagta aattatcttc
    24361 tttagaaaag cagtatgaat tacaaagtca aaaagttgaa gtaactagtc aacgtttagc
    24421 caatgccaaa aaatattatg gagaaaattc caccgaagtt cagaaacttg aaagagaact
    24481 gattaatcaa caaacagcac aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc
    24541 actagctcaa gcaaaaggcg aaatacagac gtacgagtct acaatgcaac agttggacag
    24601 tgaacagaaa aatgttcaag ctagtgcttc tctgattgaa tccgaataca aaaaatggca
    24661 agcaactgct ggtcaatcag cttctgaatc cgagaaatta gcgaaagccc aagaatttgt
    24721 ttctcaacaa tctgaaaatg cggagaaaac gatagatatc ctaagacgac agttagaagc
    24781 tacacagtct gaatttggcg ctacatccac agaagcaatg cagatggaag cgaagcttaa
    24841 tgatgctgaa cgtgaatttg aagagttagg acaagctgct aaaaatgtag atacaactaa
    24901 cttggacgat atcggaagca aaatagatat gaataatcta atggaagctt ctgacgtttt
    24961 aagcgacatt ggcgataagc ttacagaatt agggaaacaa gcagtggact ctgctaatag
    25021 tgtaggtagt tcccagagta aaatacaagc taattttggt ttgactaaac aagaggctga
    25081 agaattaacg aatgtagcca gagacattta ttataaaggt tttggagaat cgttagatca
    25141 gtccacagat gcattgattt tggtaaagcg taatttaggc gatttaaata atcaagattt
    25201 acaaaatatc acggaacaag ctatggtcct agaaaacacc atgggtgctg atatggatga
    25261 aacgttacgt ggtgtaaatg gcttaatggt caatttcggc ttgagcgctc aagatgcaat
    25321 ggatttaatg gtttcgggta ctcaaaacgg tttagataaa acgcacgaat taggcgacaa
    25381 tatggcagaa tatagccaat tatggagtca aatgggatat tcagctgatg aaacgttcgg
    25441 aatgcttcaa aatggtttag atgcgggtgc ttataacctt gataaagtca atgacttagt
    25501 taaggaaatg ggaatatcgt taacagatgg tcgatttgag caaaacatgg atatgtttag
    25561 tgaaagtact agaaaagctt ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt
    25621 tattaattcc atgattcaag attttagcaa tatggatggt caatacgacc aattaaataa
    25681 agcttctaca atttggtctg cacttggcga agataacgcg atgaaagttg tccaatcttt
    25741 gactgatgtt aatcatacat ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga
    25801 ttctactacc ccgttgcaag agttgaacgg taaaatagct gaattaaagg attcattagc
    25861 tcctataggc aacacaatca tagatgcact cgaaccagta attgattttc taggaaagat
    25921 ggctgatgcg tttaataatc ttccacaacc agtacaggat tatgccgtag cgattggcgg
    25981 attgactgct gcatttactt tattaatgcc aataatagtc ggcttcatgg ctctaggtgg
    26041 tcctactaca ttaataatag gagcagttat tactgttatt gctggagtta tagcaattat
    26101 aaaaaactgg ggcgcaatta ctgactggtt taagggaata tggagtaaat ttactgattg
    26161 gttgggtgat acttgggaaa gtataaaaga aggtgcctca tcagtttggg atggagtcaa
    26221 agaaacctgg tctggatttg tagattggat tcaagatatt tggcaaggtg tttctgattg
    26281 gtttggagag ttatggagcg gattagttga aggagcttcc aacatctggc aaggagtcca
    26341 agagacttgg caaacattcg ttgattgggt ttcaaatatt tggaacggag tcaaagaagt
    26401 atggtcgatt atttgggcag atattgtagg gattgttcaa ataccatgga cattaataac
    26461 gtcattgatt caagctggta ttaatattat cgtgggtatt tttgatgtag ctggacagtt
    26521 attaggcgca gcttggcaag ctgtttggac acctatttct gatttcctta aaaacacttg
    26581 ggatactatg acacaatggg taagcatcgc ttggaatgga attgtaacta cattccatac
    26641 tatatttgat ccagttgtgg catggtggaa cggtatatgg acatccatta gtactacggc
    26701 ttcaaatatt tggaattcaa ttagtgcaac agcttctagt atttggaaca gtatcaagaa
    26761 tacaatcact agtttggtac aagcagctgc tacagtaatt caaaatgttt ggtcaactgt
    26821 atctagttgg ttaggtggaa tttggaattc aatcagctct acagcatcaa atatctggaa
    26881 tagtgttaca agcagtataa acaacgctat aaatgcagcc aaaagcgtga ttcaaagtgt
    26941 ttggaatagt atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa acactgcttt
    27001 gaatctttgg aatggaatta caagcactat tagctctaaa gtaaacgatg gaaaaaatgc
    27061 aatttcaagc ggttggtcca atctaacagg tattgtttcc gacatattca ataatgttaa
    27121 aagtacaatt gctaacatat gggaaggcat caaaaagact gttagcgctc caattgattg
    27181 gattagagac aaaatcagtg gcatttttga taatttgaat atttcgatac cacatattcc
    27241 gttaccacat tttaaattga gtggggaatt caatccattg aagggaaaaa tcccaacgtt
    27301 gggtgttgat tggtatgcga aaggtagtgt gtttaattct ccgaatatta tcggtgtagg
    27361 cgaagcagga cctgaagcag ttttaccttt gaaaagatct gtgctgcaag aaattggtga
    27421 tcgtatcttg agtagcacct cagtttcatc tagggcgcag acggttcaac ctgtgaacaa
    27481 ttacgaattc aatttcacaa ttgatggtaa cgcagatgag gttactatga agcagacaac
    27541 tcaacaaatc attgatagca ttacaaaagt tcaaaatgat aatgcttcgg catggcgtta
    27601 aacaggagag tatttctcct gtttttttag tattaaaaag gatgtgaaaa aatgactgat
    27661 tgtatacatt ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa
    27721 agaccaacca taccatctcc aaaaagacag agagttgaaa cttctgtttt aggacggtta
    27781 ggtggcttag tgcaagatta ttcgtttgaa gatatgtcgt ttactttgca ctataactat
    27841 ttagaggatg tggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg
    27901 ttaaactatg caaaaaaatt agaattctct gatgatccca acgtctatta tgttatccag
    27961 actatcgata ttggggatgc agaaaacgat atcgttgaat ggggagagtt cgatgtaaac
    28021 attactgcga aaccattcgc aagagttcaa gaagatgtac ctataaccgt agataaacca
    28081 cggtcattta acttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact
    28141 ccttcagcta cttcatgcca gttcatctta aatgattatg tgtttagttt tgaaggctta
    28201 gtagtaggaa ctgacgtagt cattgatagt gatttgatgc tttgctacga agagcaatca
    28261 gacggagata ttttagatcg gtccaacaaa atgaagacca tgcaatatcc gacattgcaa
    28321 gtggatatca atcattttaa ttgcactggt ttgagcaaaa tacaaattta tcgtaatggg
    28381 ttaaggtagg tgaaatagat gatcgataat ttaataacta tttacgataa aaatgacgcg
    28441 aataatttag ctgaacattt atatgatacg caaggtttag gcgctttgtc agactggtta
    28501 acagccactg ttagcaataa actaaacgga gccgagatat ttcagggtac ttatccaata
    28561 agcggaacta atgcagattt gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa
    28621 aatcgagcaa aacaacgtct acggatctat tatgcaaaga cttctgtaat aggaaatacg
    28681 atagaagtaa aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat
    28741 gacagcggaa cagaaaaaat cactgctact caggcatggc aaaatgcaaa agttttagcg
    28801 aaaccagcta ttccttcgca gttttctttc tcgtcattag tagatacgct tgctaatgtg
    28861 aagatagaaa aggcgaattt tttagaattc tttggtggaa aagagggatc tattttagat
    28921 cgatttcatg gtgagtttct aaaagataat aacacattac gtcatgaaac aaggctaggc
    28981 acggatcata aaatcaaagc gatttatact aaaaacttaa ctggtcttga cttagagata
    29041 gatgctcaaa gcgttttagt tggagtttat ccattcatta gcagttcttc agaaggagaa
    29101 gacgagatca ctctaccaga agaagtcatt ttcacggatt acgtggaaga ttatcctgct
    29161 ggatatgttt cttttgttga ttttaaagac aaagcgactg atgtagcctc attaagggaa
    29221 gctgctaaaa actggttgaa aacaaacata gataaacaaa aaccacaagt gagtggttcg
    29281 attgaattgg taccattgag gcatcagaga ggctatgaaa aatttgttga tttagaaaaa
    29341 gtttcgatgg gtgacggact agatgtgtat catccacagt taaaagtgaa tatgtcagca
    29401 agaattgtgg aatatacgtt taatgtttta actaattcat acgataaatt agttgtagga
    29461 aacgtcaaaa caaacttctt agaaaataca gagaataatg taagcaattt gattaatgat
    29521 gccattgatc aattgaaaaa cggtggcgaa atcagtgatt taatcaatga tattgtagat
    29581 catcaaactg atatgattac tggtcaagat ggtggctatg ttttattaga tcctaaagaa
    29641 gcacctagtc gtattttgat tatggacacg ccagataaga ataccgcacg gaatgtttta
    29701 caaatcaaca atgctggtat tggtttctct aaaactggta ttaatggaac atatgaaacc
    29761 gcatggacgt tagacggcgg gttcaatgcc tcgtttatta cagctggtga gatagtaggg
    29821 attactatta gaggtactac attaattagt gatggtgctg attataaaac aagtattgct
    29881 aatggtaaaa taacttggca ttcaaaaaaa gttgataaag atattatgga actagaagca
    29941 cgtgatgata tagatgccga cgtaggtatt gtatcatata ccatgaaaac tggcggtggt
    30001 ttcatgatta gaaatccaca gggtaacttg gtttttagta cgtgggataa tggcaataac
    30061 agaccgtttt tatcttttgg tgcgccaaat ttcagatata gcaatgctag ttatgtaact
    30121 tctggcgacg gtaattcttt gggtatagat ggtagtgcgg gtaattcatg ggaatttaaa
    30181 gtagctggta ggactatgaa atttactagt gacggtatgc taacgttgcc aggttgtttt
    30241 tttggttcat gggaagatgg gaaacttgct aggtttgaac aatcaacggt acaagtatat
    30301 aaagatttta ctgttagagg tactaaaaac tcaaccgtac caacagaaca ttatggacaa
    30361 cgactattaa acgcttatga aactccagaa tattatttcg ctgattatgg ggaagccgtt
    30421 acaggtgatg atggtaaagt tcgtgttgat attgacccca tgtttgctga gacagtaaat
    30481 ctaagtcggt atatgacaca tgtgacacct acagaactag ttttgtgtgc tgttactcat
    30541 gaagatgttg accatttcat cattgaaact agtaagccaa acgtattggt tagatggaat
    30601 ttagtggcac atcgactagg gtatgaagat gttagattaa aagaggatac agcatatgat
    30661 agcacagtgc ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta
    30721 gcagtttata taatttggct ttagatttca gcaaagaatt aaactacacc aaagctatta
    30781 tggctcgtca aggtgataaa gggatcacgg tgactgttaa accgttttta aatggcttgc
    30841 agatggatac gagcggcgga acatttactt taaaaggaac tacgccatct aaccgttacg
    30901 tagatagtgt cgctactagt gtaactagtg aagaagtcac attttctctt gatggcacat
    30961 ttatgagtga agcagggtat tataaacact gctatgtaga atatagaaaa gacgatcaaa
    31021 ttttaacgac gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc
    31081 aagccgatga atatgtttcg caattagaag aattgattcg aaagtataac gaaacttttg
    31141 atgcttttct ggctgaaatt aaaggtagaa tggatagctt aaataaacag attactgatt
    31201 taactggtca agctaaaaca ctacaagaca agttagatgc tctgaaagaa gaaatttcta
    31261 aattaggtaa cttacatgtg atgtactcca acagcatcga attcgggggc tatgattatt
    31321 cggggaatcc gaattttcta agtaagctat catatgactt ggtggtaaat caaaatggtt
    31381 ccgttggaac agtttctcag ggtgaaaact cgtttaaata taataagaca actaaggatg
    31441 tagacggttc agtggcttta tactataagg caggaggtag agctaactgg ctaccatcca
    31501 ataaaagaat agtaatgacc gttaagctta gagctggagt tggatatatt ccagcggatg
    31561 ggagaaaagt actgattaga tataggtata ttgacaatgg aactagtaag atacctgtag
    31621 acttacaggt taacagtgct tcactaactc aggagtggca agagtttact gttactggaa
    31681 ctactcaaac gtttagccaa caagcaaacg atccttgggt acaattttat gttcagatgg
    31741 ggatacttgg ggaaatagaa atgagctatg acatcaaaat cgaagaaggc tcaacagcca
    31801 ccccgtacca gcctaactta cttgatgcgc cgtattattt gagtaaggtg gctttgggtg
    31861 agaatattgc aaataaaact attaaatatc ctattaaaac aagtgcttat gcaacatata
    31921 cagcttccaa tatagaaaat tatcaggcta accaaactta cacattaact atgaaagcta
    31981 ctaaaccagc tacacaaact tttggtgttt ttctaaaagg tggagctcgt ggtgttggta
    32041 atatggtacc agttgaaggt aaaacagacg aatggtcact atcttttaca gttacacaag
    32101 cccatattaa cgcaggggta actcgtgatt taagtattta tcaattgcca caagccacaa
    32161 ttggcgcatg ccaaattgac tggcttaaaa ttgaaaaagg cgacacccga actccgaata
    32221 ttgagcaata taaataccga ggaatcggca tgcgagactc aaacaatcca aaagattatg
    32281 tatgggatct agaacctagc tatgttgaga ataacctagc tacagatatc aaggttgctg
    32341 aaatcacagg taaagcaaac aattataccg atgggaaagt atcggagatt aattcgcagt
    32401 tgacagcttc aattaatgaa gtagatacca cagctaagga tgctcaaaca aaagcgaacg
    32461 ctaatgagac tgctataaag agtacgaata gtagggttaa tgagatagaa aacattatta
    32521 cggccctaac aaaagaagaa acatatttgc tacctgctag tatagtaagt ggcggatctc
    32581 ttaaattcac aagtcgtggt ggaatgacag tagctacagg atcatttaag ttggctaagg
    32641 acatagcatt cggagaagtt ttagttaatg atttaccaga ttcaatgatt aatattgata
    32701 acaatgctat agggactgga gctgtcacag gtaattctgt atcgcaaata tatgttgaat
    32761 ctaatacgag aaaggtggtt gcgaataccg ctatgaaaaa aggtcaatgg tttacagttg
    32821 gattttcgta tcacagtaaa ttatctatat agatttagcg tactcattcg agtacgtttt
    32881 tattttgcaa tgaaaggagg ctagttggtt gaaagatgaa gcaatacaag acgttgtgga
    32941 gagattagta agaattgaaa caaaattaga caactacgaa tcacttagag aaaaggctga
    33001 tagtgcaaaa gatttggcag ataaagccta ttcagtagca ctaaacaatg cagaagacat
    33061 caaggaaatg aagaataata ataaatgggc ttggggctat atgattggct taggcattac
    33121 gatcattggc tatttcttga ctaaattgta aaggaggtga gaagaaatga ttttacccga
    33181 taagtattat caagtcatta aatggacggt tttaacagtt ttaccagcta catctgtttt
    33241 agtagccacg ttaggaaaag catatggatg gaatggaaca gatatgacag tactcactat
    33301 caatgcagca gcaacatttt taggcgttat cactggtgtg tcggcatata atttgaaaaa
    33361 ataggaggaa aaaaatgaag aagaaaatca ttttatcatt gagcctaata atggctcttt
    33421 ttttattgcc aattaacggg ttcgcctata cgatcaataa tgaatttaat ttaggtgcaa
    33481 atgaaggtag ctcacaagta gcaaataatc agtatatttt actgcatgaa acggctaatg
    33541 aaacagcaac aggacgcaat gaagcgcagt atatgcaacg ttcatggact agtgcttaca
    33601 ctgcttacat tgtgggagac ggcggaattg tttaccaagt cggtcaacct ggttatgtac
    33661 agtacggcgc tggttcatat gctaatgcta acagtcctgt gcaaatcgaa ttacagcata
    33721 cacatgacaa agcgactttt gaaaagaact ataaagcata cgttgaactg gcaagagatt
    33781 cagcaatgaa atatggtatt ccattaacat tggacactcc ttataaccaa ccaggaatca
    33841 aatcacattt atgggtaaca caaaatatct ggggagatca tactgatcca tacggttatc
    33901 tttctgaaat gggtgttagc aaagaaaagc ttgcttatga tttagctcat ggatttacag
    33961 atgacaatcc gacaacttca gatgataaac cagtcattga tccaactcga gcaggtgctg
    34021 caaatcctac gctgacagat ggaacaaatt acgcccacat tgatcagttt ggagaaatcg
    34081 aaaacgcaaa cttgcatgta gctggttggc acattgctaa ctataaatac gagtatattt
    34141 tcattatgga ctacaatact gggaaagaat tagctcgagt aagagctgat ggaatttata
    34201 gaccagatgt aaatcaagct tataatactt ctggaaacgt tggttatcat gtatctttca
    34261 atatgcgtaa ttttcctaat aagaaagtct atgtaatgat gcgtgcaacg aatgatccag
    34321 aagggaacac taaaggcgga gcacaagatt ttcatgataa gcgttggtat ttaaatattc
    34381 cacaacgata aaaaaatagc ccctcgatga ggggcggtac ataaaattct tatataaata
    34441 atactatagc ctgtataact ttcatttaca atagatcaga acgctcaaat atataatgat
    34501 ttcatcaaat ttactctaaa actgtggtat gctagtttta gtaactattc atatttggaa
    34561 aaaaggattg agcatatgaa cgaaaaggga aaatctattg atggcttaat gagacatatg
    34621 agaaataacc ataatgtgaa aattaatggt agtagagaca aacaaaactt attaaatatg
    34681 ggatactatc acggttataa ggcttcaatg tatataaaag agagaaaaaa tatgcagcaa
    34741 ttcgaagagt tttacgaagt aaaagctgtt tacgattttg atttagaaat gaaaacaatt
    34801 ttttatccaa tgttaattaa aatagaaaca tcattaaaaa ataggttaat agatttttta
    34861 gtgaaaggag aacaatgtga cattgaatat atctacaaaa ataagcttag agactatgca
    34921 tcaaaagaag taggatccaa agattacaaa aaatatttag ctaaaaacct taagctaaga
    34981 gaaaaaatag atgaaacaat tgcttataac tacggtaacg gtcatccagc agtacaacac
    35041 ttttttcact gcaataaacc attacctttg tgggcatatt ttgagataat aacatttgga
    35101 gaattcggta attttattgg atgcttaaat aaagagctga gaattaattt tgctgaaagc
    35161 ataaatttac atcatagtgg ttttaatcaa aatggaagag ttatagaaaa tatagtattt
    35221 tgtttaacag gacttagaaa tgctactatg cataactctg taatttttga ttgtagattc
    35281 aataattcta atacttcaaa tcagcttaaa ggatatttgg aaaaggtaac agggataaaa
    35341 aatattacat ttgatacgtt gatggatttt atgattttat tggttatgtt gttgaaaaaa
    35401 attaatataa ctaagaatga gttaaatagg tatgtgagac actttgatga tattagagaa
    35461 acactttttt taagtgttcc gtttagtacg tatacagcta taataggaac agatgcaaaa
    35521 aataaaatag ttgctcttaa aaattttatc cgaaaataag cgaaaattgt ttacatttta
    35581 tattctatat gggataattt tattatcaaa tagcggtaca ctacttcggt ttgtacctaa
    35641 aacactccat gcgtggggtg ttttttattg tgttagtaat ttggtctcaa tatagagtag
    35701 cctatcaaat aaatatagat aaaaacctca accaatcatc cttggctgag gttttttcat
    35761 tttttctgaa aaagttaaga aaataaataa aaaaataatt tatcttttct caggtccatt
    35821 ctttttgttt gcagtagtcg tgatggcttc tcgt
//
LOCUS       DACIRY010000014        35388 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.014, whole genome shotgun sequence.
ACCESSION   DACIRY010000014 DACIRY010000000
VERSION     DACIRY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.014"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(3..1529)
                     /locus_tag="IYE22_000581"
     CDS             complement(3..1529)
                     /locus_tag="IYE22_000581"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ9505815.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(1544..2191)
                     /locus_tag="IYE22_000582"
     CDS             complement(1544..2191)
                     /locus_tag="IYE22_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ9505816.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(2375..2704)
                     /locus_tag="IYE22_000583"
     CDS             complement(2375..2704)
                     /locus_tag="IYE22_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ9505817.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            2881..3609
                     /gene="sfsA"
                     /locus_tag="IYE22_000584"
     CDS             2881..3609
                     /gene="sfsA"
                     /locus_tag="IYE22_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ9505818.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            3625..4638
                     /locus_tag="IYE22_000585"
     CDS             3625..4638
                     /locus_tag="IYE22_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9505819.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            4638..5915
                     /locus_tag="IYE22_000586"
     CDS             4638..5915
                     /locus_tag="IYE22_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ9505820.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            5978..8680
                     /locus_tag="IYE22_000587"
     CDS             5978..8680
                     /locus_tag="IYE22_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9505821.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            8832..9149
                     /locus_tag="IYE22_000588"
     CDS             8832..9149
                     /locus_tag="IYE22_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9505822.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            9179..9499
                     /locus_tag="IYE22_000589"
     CDS             9179..9499
                     /locus_tag="IYE22_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ9505823.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            9607..11067
                     /locus_tag="IYE22_000590"
     CDS             9607..11067
                     /locus_tag="IYE22_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9505824.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            11135..11356
                     /locus_tag="IYE22_000591"
     CDS             11135..11356
                     /locus_tag="IYE22_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505825.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            11387..11569
                     /locus_tag="IYE22_000592"
     CDS             11387..11569
                     /locus_tag="IYE22_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ9505826.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            11569..11982
                     /locus_tag="IYE22_000593"
     CDS             11569..11982
                     /locus_tag="IYE22_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ9505827.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            12105..13286
                     /locus_tag="IYE22_000594"
     CDS             12105..13286
                     /locus_tag="IYE22_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9505828.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            13675..13748
                     /locus_tag="IYE22_000595"
     tRNA            13675..13748
                     /locus_tag="IYE22_000595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13709..13711,aa:Arg,seq:cct)
     gene            complement(13785..13985)
                     /locus_tag="IYE22_000596"
     CDS             complement(13785..13985)
                     /locus_tag="IYE22_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ9505829.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(14290..15522)
                     /locus_tag="IYE22_000597"
     CDS             complement(14290..15522)
                     /locus_tag="IYE22_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ9505830.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            15776..16345
                     /locus_tag="IYE22_000598"
     CDS             15776..16345
                     /locus_tag="IYE22_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9505831.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(16523..16963)
                     /locus_tag="IYE22_000599"
     CDS             complement(16523..16963)
                     /locus_tag="IYE22_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ9505832.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            17121..17885
                     /gene="map"
                     /locus_tag="IYE22_000600"
     CDS             17121..17885
                     /gene="map"
                     /locus_tag="IYE22_000600"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ9505833.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            17917..18840
                     /locus_tag="IYE22_000601"
     CDS             17917..18840
                     /locus_tag="IYE22_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ9505834.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            18916..20055
                     /locus_tag="IYE22_000602"
     CDS             18916..20055
                     /locus_tag="IYE22_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ9505835.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            20048..20848
                     /locus_tag="IYE22_000603"
     CDS             20048..20848
                     /locus_tag="IYE22_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9505836.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            20848..21675
                     /locus_tag="IYE22_000604"
     CDS             20848..21675
                     /locus_tag="IYE22_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9505837.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            21653..22387
                     /locus_tag="IYE22_000605"
     CDS             21653..22387
                     /locus_tag="IYE22_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ9505838.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            22487..23353
                     /gene="rfbA"
                     /locus_tag="IYE22_000606"
     CDS             22487..23353
                     /gene="rfbA"
                     /locus_tag="IYE22_000606"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ9505839.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            23367..23939
                     /gene="rfbC"
                     /locus_tag="IYE22_000607"
     CDS             23367..23939
                     /gene="rfbC"
                     /locus_tag="IYE22_000607"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ9505840.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            23961..24989
                     /gene="rfbB"
                     /locus_tag="IYE22_000608"
     CDS             23961..24989
                     /gene="rfbB"
                     /locus_tag="IYE22_000608"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ9505841.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            25087..25938
                     /gene="rfbD"
                     /locus_tag="IYE22_000609"
     CDS             25087..25938
                     /gene="rfbD"
                     /locus_tag="IYE22_000609"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ9505842.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            25973..28006
                     /locus_tag="IYE22_000610"
     CDS             25973..28006
                     /locus_tag="IYE22_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505843.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            28050..29330
                     /locus_tag="IYE22_000611"
     CDS             28050..29330
                     /locus_tag="IYE22_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ9505844.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            29540..30346
                     /locus_tag="IYE22_000612"
     CDS             29540..30346
                     /locus_tag="IYE22_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9505845.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            30358..31578
                     /locus_tag="IYE22_000613"
     CDS             30358..31578
                     /locus_tag="IYE22_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505846.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            31568..33154
                     /locus_tag="IYE22_000614"
     CDS             31568..33154
                     /locus_tag="IYE22_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9505847.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            33193..35331
                     /locus_tag="IYE22_000615"
     CDS             33193..35331
                     /locus_tag="IYE22_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ9505848.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT        11212 a         6067 c         7356 g        10753 t
ORIGIN      
        1 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
       61 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
      121 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
      181 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
      241 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
      301 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
      361 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
      421 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
      481 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
      541 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
      601 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
      661 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
      721 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
      781 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
      841 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
      901 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
      961 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
     1021 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
     1081 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
     1141 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
     1201 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
     1261 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
     1321 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
     1381 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
     1441 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
     1501 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
     1561 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
     1621 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
     1681 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
     1741 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
     1801 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
     1861 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
     1921 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
     1981 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
     2041 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
     2101 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
     2161 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
     2221 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
     2281 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
     2341 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
     2401 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
     2461 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
     2521 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
     2581 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
     2641 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
     2701 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
     2761 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
     2821 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
     2881 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
     2941 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
     3001 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
     3061 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
     3121 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
     3181 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
     3241 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
     3301 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
     3361 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
     3421 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
     3481 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
     3541 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
     3601 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
     3661 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
     3721 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
     3781 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
     3841 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
     3901 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
     3961 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
     4021 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
     4081 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
     4141 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
     4201 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
     4261 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
     4321 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
     4381 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
     4441 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
     4501 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
     4561 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
     4621 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
     4681 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
     4741 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
     4801 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
     4861 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
     4921 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
     4981 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
     5041 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
     5101 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
     5161 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
     5221 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
     5281 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
     5341 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
     5401 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
     5461 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
     5521 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
     5581 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
     5641 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
     5701 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
     5761 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
     5821 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
     5881 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
     5941 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
     6001 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
     6061 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
     6121 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
     6181 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
     6241 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
     6301 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
     6361 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
     6421 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
     6481 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
     6541 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
     6601 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
     6661 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
     6721 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
     6781 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
     6841 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
     6901 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
     6961 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
     7021 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
     7081 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
     7141 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
     7201 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
     7261 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
     7321 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
     7381 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
     7441 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
     7501 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
     7561 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
     7621 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
     7681 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
     7741 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
     7801 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
     7861 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
     7921 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
     7981 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
     8041 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
     8101 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
     8161 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
     8221 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
     8281 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
     8341 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
     8401 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
     8461 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
     8521 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
     8581 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
     8641 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
     8701 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
     8761 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
     8821 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
     8881 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
     8941 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
     9001 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
     9061 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
     9121 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
     9181 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
     9241 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
     9301 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
     9361 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
     9421 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
     9481 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
     9541 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
     9601 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
     9661 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
     9721 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
     9781 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
     9841 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
     9901 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
     9961 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    10021 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    10081 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    10141 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    10201 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    10261 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    10321 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    10381 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    10441 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    10501 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    10561 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    10621 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    10681 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    10741 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    10801 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    10861 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    10921 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    10981 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    11041 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    11101 gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca gtgcaatgca
    11161 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    11221 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    11281 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    11341 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    11401 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    11461 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    11521 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    11581 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    11641 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    11701 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac
    11761 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    11821 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    11881 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    11941 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    12001 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    12061 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    12121 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    12181 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    12241 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    12301 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    12361 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    12421 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    12481 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    12541 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    12601 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    12661 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    12721 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    12781 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    12841 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    12901 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    12961 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    13021 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    13081 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    13141 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    13201 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    13261 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    13321 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    13381 aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc
    13441 ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt
    13501 ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag
    13561 tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta
    13621 gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct
    13681 gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt
    13741 caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt tgtataagta
    13801 gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact
    13861 ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga
    13921 gaaaataggg aaaaattgat tactaaagaa aaacttacta ggtcgacggt gtagttgatt
    13981 ttcatacttc aatagattcg aattaaacga tttcgattgg cttataccag tcctatccaa
    14041 cgaaagatat atcgttctta actctagcgg tataacttga agctttacgt aaagccaatg
    14101 ggaatgaact ggagatgact ttttattatt tttagtaata gctgatgggt taatacttac
    14161 aactttcctt ttcaaagtac catataaaaa aacggtgggg ttgtttagcg aaataaagga
    14221 tctaagccaa tagcttagat cctttattct atgctttata ttattttttg catgtgtgcg
    14281 aaagtatact caagcccaat cgccatttcg gaaaatcggt actgtagagc catcttcacg
    14341 gattccgtcg atatccatct tgtcagatcc taccatgaaa tctacgtggg tttgacttct
    14401 attcaatcca gcttctgcta attcttcatc ggacatttct gtaccgcctt tcacactgaa
    14461 ggcataagca gaacctagtg ctaagtgatt cgatgcgttt tcatcaaata atgtattgaa
    14521 aaagatgatg ccagattgag aaataggtga agggtcaggt acaagtgcga cttctcctaa
    14581 acgacgcgca ccttcatctg tatcaagcag ttttgctaag acatcttctc cttgttcggc
    14641 agaaaaatca acaacttttc catctttaaa agtaaatttc atacccgaga tgatggtgcc
    14701 agcataactc agtggctttg tacttgaaat gtaaccatct actcgatgac tgtcaggtgc
    14761 tgtgaacact tcttccgtcg gcatatttgc catgaatttt tctccacgag cattatagct
    14821 tccagcgcct tcccaaagat ggtttttagg caatccgata atgatatctg taccgggagc
    14881 agtatagtga agggcagaaa attgttctcg attcaattct tctgcttttt ttgctaattt
    14941 ttcatcatgc ttcttccatg ctaagacagg gtcttcttca tagacacgag tcgttttgaa
    15001 aatctgatcc cataatgcct caacttgttt ttcttctggc agttctggga agacttttgc
    15061 tgcccattgt ttaccagccg cagccacgac agtccaactg actttatttg cttgagttgc
    15121 cttgcgtaaa ttcattagtg ctttgccatt cgctgattga taagacgcta ctcgatggct
    15181 atctacacca gcaaatgcat cgggatcggc agaaacaacg ctgatcctgc ttgctccttt
    15241 ttcaagccat tcatccgctt gatcgatttt agattgtggt acattctcaa tacgatccgt
    15301 tgcagcatgt aaaagaaatt ctctttggat ctggtcgtca gtccattgaa cgataacctc
    15361 agcggctcct agtttataag cttcttgtgt gatcaatcga gcaaggggtg cctgttccac
    15421 gctgatctgt aaaacaactg tgtgtccttt ttctgtggct acgccagttt ctgcaattaa
    15481 gcgtgcatat ttttttagaa gttcattaaa atcagataac ataataaaaa ttcctcctca
    15541 ccattttttc tggtaactat tagcatgata ccatttaatt atgattcaaa caatgaggct
    15601 ttaaataaaa aaaggaggtt atttttttaa ggttaagttt catgatatat gttactactt
    15661 gctatttcag cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa aacatgttac
    15721 aatgaaatta atcaatggtg gaagcgttat aaaaaaatta aggagattaa aaaatatggc
    15781 aagaaaaaag acgattacta gagaacagat tttggcagct gcttatgaag tagctgccac
    15841 cgatggattt acacgattta ctgcgagaaa catcgcgaac aaaatgaatt gttccacgca
    15901 accgatttat cttgagttta aaaatatgga tgacttgaga caagctcttt ttgagcagat
    15961 ctatgatcat ttacaatatg aagtgttcac tgttgagcat acagggaata cgattatcga
    16021 tcttgctttt aattatatcc attttgcaag aagagagaag aagttgtatc gttcactgta
    16081 cttagaagaa tatggtggag gaaaagagat gcagaatttc tcttatactt actttgtgaa
    16141 tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa gagcagatcg aatctttgta
    16201 taatgggaca tggatcatag ctactgggat cgcagctttg atgtcttcaa gcatcatcca
    16261 tccaactgat gaacagattg aaaagatgat ccaagacagt atagatgcta tcttgaaatt
    16321 caaagaacca atcgaaatcg attgaatata ctcaaaagtt ccacaaatca gtaggtaaca
    16381 agagatgtgt caaaataagg aaaagttcgt ttttcttaca gcgtatgcat ttttaactcc
    16441 cgaagaactc ttccatgaat gcaatgaata aaagagccat acagattgaa taaatctgga
    16501 tggctctttt attgcaagta tattaaattt ttgcgactag ttctttagcg aaagcttcta
    16561 aattttcgat atcttcttct tcagcattca gatccacttt aacattttcg gctccttttg
    16621 atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac cgatttgcag aattcatcgt
    16681 aaaaagtatc accagatccg cagacaccgt aaatttttcc caatagatcc aattcttgca
    16741 agtcttcata aaagtctacg atctcatcag gcagatcgcc atcatcgtat gtataagtag
    16801 ctactacgca aatatctgcc tcctcgaatt catctgcatc tacttgggta cattcgttga
    16861 tttcgacttc gatatccaga ttttcaaatg cttctgcaac aatatcggcg atttcttcag
    16921 tgtttcctgt catgctagca tatacgattt ttgctaaagc catgatgtgc ctcctcgttt
    16981 ataattattt aacaattatg aattatacca aaacatcatt gaaaagaaaa taaaacctaa
    17041 agacacctgg ggaatgtcat ttggctaaat gtatgccgct atgttaaaat gagagggatt
    17101 tgtaaaaagg agacgaaaat atgattactt taaaatcagc acgtgaaatt gaaatgatgg
    17161 cagaatctgg agctttatta gcagatgtac ataaaaatct gcgtgatttt ataaaaccag
    17221 gaatcacaag ttgggatatt gaggtatttg tcagaaacta tatcgagagt catggcggaa
    17281 ttgctgcgca aattggcttt gaagggtatg aatatgcgac ttgtatcagt ataaatgatg
    17341 aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa cggtgatttg gtaaaagtcg
    17401 atatgtgtat cgatttgaaa ggcggcattt ctgattcttg ttgggcctat gtagttggag
    17461 aatctacacc tgagatcgac cgattgatgg aagtaacgaa gaaagctttg tacatcggta
    17521 ttgagcaagc acaagtaggg aatcggattg gtgacatcgg tcatgctatc caaacttatg
    17581 tagaaggcga aaatctagcg atcgttcgtg attttattgg tcatggcgtt ggtcctacga
    17641 ttcatgaaga accagcagtt cctcattacg gagaagctgg aaaagggttg cggttgaaag
    17701 aaggcatggt tatcacgatc gaacctatgg ttaataccgg aacttggaaa atgaaaatgg
    17761 atcctaacgg ctggactgcg tatacaagag acggcggact ttcttgccaa tatgaacata
    17821 cattggctat tactaaagat ggtccaagaa ttttgacatc acaaggagaa gaaggcactt
    17881 attgatctat ccagtcaata ggtacgggag aggtacatga aagtttggaa caaaataaaa
    17941 caaaatgaaa atctgatgcg ctttattgag acgactcaaa agcgcatcac tgattcagaa
    18001 atgagtacga cctctgtcgt cgttgcttat tatttactct tgtctttatt tccattgatt
    18061 attgcttttg gtaatatttt accgtttctc cacatcgatc aagagactgt actgacctat
    18121 ttacgagaag tgattcctga gaccgtttac cagtttatcg gtcctgcaat caaagacctt
    18181 ttgacacaaa gttcaggagg cttgttatcc gtttctgcat tagcggcttt atggtctgca
    18241 agtcaaagta tcaatgcttt gcaaattgcg atgaataaag cgtatggcgt agaaaataga
    18301 aaaaattttt tgatcgtccg tttcttctca ctaattgtca ttgtcttgtt catgattgct
    18361 attagtgggg taactatcgt tcttgggttg ggacagctga ttttggatgc tattcagcca
    18421 atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa ctttgaagtg gccaatcaca
    18481 ttggtagctt tatttgtcat catgttcttg atttatttaa ttgtccctaa tgctcagttg
    18541 aagttgaaga cgatcattcc gggcacgatc ttcgctactg tcggctggat gctgttatca
    18601 caagtgttta gtatctatgc taggtatttt gcaacgagga tcagcggtta tcagatcata
    18661 ggaagtttta ttgtactcat gctttggctt aattttgctg ctacgatcat cattttgggc
    18721 ggaatcatca atgcagttgt tcaagaatac gtgacgggtc atcaaatcaa aaaacgtcga
    18781 gatccaagcc gaaaatggtt caataaattg aaaactaaat tttctcataa aaaaaagtag
    18841 aaacaagagt tcttattgta aatttatatc agtaatttat aataattatg actcttaatt
    18901 aaagtaggtg cccatatgct tgtttctttt agtatggtta taagattttt tctgacaatt
    18961 ttattatcac tgatattaac acctgtattc aaaatcattt cagtacaaac aggtatggtc
    19021 gacaaaccaa atgaaagaag aataaataaa gttccaatgc cttcagcggg cggactgcct
    19081 atctttattt catttgttat atcgacttta ttcttattcc gtaaaattat ccctcaatcg
    19141 tatattgtac cgatcttgat tgcttcttta gtaatcatag tcacaggagt gctcgatgat
    19201 aaatatgagc tgacacctaa acaaaaaagt atagggatct tgattgcttc tcttattatt
    19261 tattttgtag cagatatacg tattgattcc ttcacgcttc cttttattgg atatatccaa
    19321 ctaggctggc ttagttttcc cgtaacgatt ttttggatct tcggtatcac gaatgcggtc
    19381 aatctgattg acggtttgga tgggctggcg gcagggattt cattgatcgg tttaattacg
    19441 ataggaatca ttggatattt tttcctccat gcttctacag tgtatattcc agttgttatt
    19501 ttctgtcttg tagcaagtat tatcggtttt tttccttaca atttttatcc ggcaaagatt
    19561 tatttaggcg atacaggagc tctattccta gggtttatga tggccgtcct ttcactacaa
    19621 gggctgaaga acgtgacttt tgtttcatcg atttctttgc tgatcgtcat gggagtgccg
    19681 gtaacagaca cgttttttgc tatcattagg agaaaagcaa accgtgtttc tttttctaca
    19741 gcagataaaa aacatcttca tcaccgacta atcagtttag gctttactca taaaggtgct
    19801 gtcctgacga tttatgcgat ggctttgatg ttttctttca cagctatggt catgaattat
    19861 actggaagaa tcggaacgat catattgatt attgccatgt tgtttgctgc aattttatta
    19921 tttgaattga ttggtctgat caatgagaaa catcagttta ttttaaaaac attgcgattt
    19981 ttagggaata aagagtaccg tacgcaagta aaacagaaat atttgaagaa atggaaccgt
    20041 catatagatg aataacaaat taaaccagcc gatccttatt agtattgtga cggcaaacag
    20101 tgataaaatt tttcggacac tagataattt tatttcaact gtaaaagatg aaacagcagt
    20161 aaaaatccga atttttgaca acaattcact tcccgagtac aaaaaaagac taacagagta
    20221 taggaaatat ccctacatcg atatctattt tcatgatgaa aattccggat ttggttatgg
    20281 acataatcac aatctgatag ctagcgattc tcgctatggc attatttgta acccagatat
    20341 tttagtggat gaagagacag ttacagcatt ggtcttgtta ttgaaagaac atccggaatg
    20401 tgcgatgtta gcacctaaga ttcttaatga agatggaaca actcagcacc tcatccgtga
    20461 gcagctggct gtttttgatt acgttttgcg ctttgtgcct ttcaaatggg tcaaaaaaaa
    20521 gtttgacaaa cgactggctg tgtttgaatg ccgaggattg cctactgatg ctaatagtta
    20581 tgtacggatg atctcaggta gttttatggt agctgatatt gataaattta aagagataaa
    20641 ggggtttgat gagcgtttct tcatgtactt tgaagacaac gacttgtgtt tgagttttga
    20701 aaaagcaggt tacaagttat tgtacacacc cttttatgca gttatccatt tttacgggaa
    20761 gggtgcgcat cgaaatgcaa aactgtttcg cgtgtttttg acttctatga tgaaattttt
    20821 caataagtgg gggtggcgtt ttttctgatg aaaccagaaa tattagtagt cgttgtgctt
    20881 tatcagcaac tattcagtca atctccagct tatggaccat taagcaaagc acttagcgaa
    20941 aagcaaattc agcttattat ttacgataat agtcctcaaa ggcagcaaaa tgagctgttt
    21001 gaaaaagaaa atatcgttta tcatcatgat ccgcaaaatc ctggtttagc ggtggcttat
    21061 aattttgcgt tgtctcaagc agatgaagga acacgttgtc tcgtcactct ggatcaggat
    21121 acgaggctag tcgacgatta ttttgaacta ttgcgtaaag taacctttac agatgaatgt
    21181 gttgctgcag ttccgatgat tttttctggg agccgacaag tttctcccct atattcagat
    21241 cgttatatca atcgtacagc tcaagcagta gaagttgaag gcccaacagc tgaacgaatc
    21301 atggcaatca attcaggtgt tgcctggtct gtcaaatttc taaaaaagat tggcgggttt
    21361 aatcctgctt tttctttaga ttttttggat cattggctgt tttggaaagt ggatcagttg
    21421 gaaaaaactg tggagatcct tcctgtacga atggaacatg atctttcggt tttagactat
    21481 gaaaaagttt caagtaatcg ataccgttct attttacaag cagaaaatcg attttatcat
    21541 gaatatgatc aaaggcattt atccagtcac cgacgacaat tattgttaag aacagctaaa
    21601 cagttcttga ccgtcaaaaa ccggcatatc tggcggatga ctttgcgctc atatgttaag
    21661 aattggaagg tgtgaattcg tgcgttcggt ttgtatagct acctttaatg gaggcagata
    21721 tttaagagag cagttagata gtattttatc tcagctttca gcagaagatg aagtagtcat
    21781 ttctgatgat gggtcagtag atgatacatg ggatatatta actgaatacg cagaaaaaga
    21841 taagcgaatc cagctttttt cggggcctcg tcaaggattg atcgctaatt ttggttatgc
    21901 gatccagcag acgaaaggag atctaatctt tttggcagac caagatgatg tttggttgga
    21961 gacaaaagtg aaaaagatca ctgactattt tgagcagaat ccggatcagc tagtagtagt
    22021 ttctgattta atcattgtgg acagcgagct gcaagagatc catgattcgt atttttcgtt
    22081 tcgaaattcc aaagaaggat ggcttaataa tgtgataaga agtcactata ttggtgctgg
    22141 tatggctttc cggtcttctt taaaacccga tattttgccg atcccgtcag aagtgccgat
    22201 gcatgatatg tggattggtt tgctgggagg aaagaagact gggttcctaa gagaaccgtt
    22261 aactctgtac cgaagacatg atttaaacgc gagtgagatt gaaacacatt ctaaattacg
    22321 gcaaaaaatt tcttggcgtg ttcatctgat tacggcgttg atcaaacgaa aatggtttaa
    22381 acgttgataa aagatgttta aatccttaat atttccatga caaatataac aattgatgag
    22441 ataataacaa agtgaaattt gaaatcaaaa aggagagcag atatccatga agggaatcat
    22501 tcttgctggg ggcagcggaa cacgtctgta ccctttaaca aaggcaacat cgaaacaatt
    22561 gatgccgatt tatgacaaac caatgattta ttatccaatg tctactctga tgttggcggg
    22621 aatcaaagaa attctgatta tttcaacgcc acaagataca ccacgtttca aggaattatt
    22681 tggaaacgga aatgatttag gactgcagat cgaatatgca gtacaagaaa gtccagatgg
    22741 attggcacaa gcgtttatca ttggagaaga atttattgga gatgatagtg tctgcttggt
    22801 attaggggac aatatttact atggtggcgg tctgtctaaa atgttgcaac gagcagcttc
    22861 aaaagataca ggcgcgacag tatttggcta tcatgtcaat gatccagaac gctttggtgt
    22921 agtagaattt gatgagaata tgcaagcttt gtcgattgaa gaaaagcctg ctcagccaaa
    22981 atcaaattac gctgtaactg gcctttattt ttatgacaat gaggtaattt ctatcgctaa
    23041 aggtatcaaa ccttcagaac gtggagaact agagattacg gatgtcaata aagcatattt
    23101 ggataaagga aaactatccg tagaacttat gggacgcgga tttgcttggc ttgatacagg
    23161 aacacatgaa tcattgcttg aagcatctac ctttatcgaa acaattgaaa aacgtcagaa
    23221 cttaaaagtg gcttgtttgg aagaaattgc ttaccgtatg ggctacatcg ataaggaaca
    23281 attgctggcc cttgcacagc cattgaaaaa aaatcaatac ggtcaatatt tattgaattt
    23341 ggccgctgaa taaggagtcc ttacagatga aagtaacaga tacaaaatta acagatgtca
    23401 aaatcgtaga aatggacgtc tttggcgatc atcgcggttt cttcacagaa agttattcaa
    23461 aagcaaaatt tgcagaacat ggattgaaat atgactttgt tcaagacaac cattcactat
    23521 cagctgaagc tggtgtcctt cgggggcttc atttccaaaa aggagaagct gcacagacaa
    23581 aattgattcg ggtagtcact ggagcagttt tagatgtagt agtggatatc cgcaaaggta
    23641 gcccgactta tggtcaatgg gacggattta tcttatctga acataatcat cgacagttgc
    23701 ttgtacctaa aggatatgcc catggattcg ttactttgac accaaatgtg aacttccttt
    23761 ataagtgtga taattactat aatgcagaag cagatggggg catcgcattt gatgatccag
    23821 atttagcgat tgattggcca attgatccat caagagcgat tatgtcggaa aaagatcacc
    23881 gtcatccgac attaaaagaa tttgaagcag aaaatccatt tgtttacgga gaaatctaag
    23941 aattaaggag aaattttctt atgaaaaaca tcattgttac cggaggtgcc ggattcatcg
    24001 gctctaattt cgttcattac gtggtaaaca atcatccaga agtgcatgtc actgttttgg
    24061 ataaactgac ttatgcagga aacaaagaga acttggccgg acttcctagc gatcgcgtag
    24121 aattggtcgt aggagatgtt gcagatgcag aattagtgga ccgtctagta aaagaagcgg
    24181 acgcagttgt tcattacgcg gcagaatccc ataacgataa ttcattaaaa gatcctttcc
    24241 catttgtaca aacgaatatc attggtacgt atacactttt agaagcttgc cgcaagtatg
    24301 atgtacgcta tcatcacgta tctactgacg aggtttacgg cgatcttcca ttacgtgaag
    24361 acctgccagg acatggggaa ggcgaaggag aaaaattcac tgcggaaaca ccatataatc
    24421 cttcaagtcc atattcttct acaaaagctg gttcagatct gttagtaaaa gcatgggtgc
    24481 gttcttttgg cttacgtgca accatttcaa actgttcaaa caactatggt ccttatcagc
    24541 atattgaaaa atttattcct cgccaagtaa cgagtatctt aagcggtatt cgtccaaaat
    24601 tatatggcga aggaaagaat gttcgtgact ggatccatac gaatgatcat tcttcagcag
    24661 tgtggctgat tttgacgaaa ggcagaattg gcgaaaccta tctgatcggt gcagacggtg
    24721 aagaagataa taagaccgtg atggaattga tcttagaaat gatgggccaa ccaaaagacg
    24781 catacgacca tgtaaacgac cgcgcagggc acgatttgcg ttatgcaatc gattcgacaa
    24841 aattgcgaga agagctaggt tggcagcctg agtttacgaa cttccgtgat gggttggctg
    24901 acacgatcaa atggtacgaa gaacatgaag actggtggaa aaaagaaaaa gaggccgttg
    24961 aagcagcata tgccaaaaat ggacaataat acagaggaaa tttcactgta ttaatggaaa
    25021 aacacatgtg aggcaggcgc tcgcctgtct cttttttaaa ataaaaatca aggagcggat
    25081 aaccatatgt ttttattaac tggaggaaat ggacaattag gtacagaatt gcgccattta
    25141 ttagatgaaa aaaatctgcc atatgtttca acagatgcaa aagacttgga tatcacagat
    25201 gctgagaaaa caatggctta tattacagaa ttaaaacccg atgttatttt ccattgcgca
    25261 gcttacacag cagtagataa agcagaagaa gaagcaaaag aattagatga gaaaatcaat
    25321 gtcgatggca caagaaatgt agcacttgca gcaaaagctg tgaatgcaca attcgtctat
    25381 atcagtacag actatgtttt cgatggaaaa aacaaaaatg tagaatacga agtagacgac
    25441 caaacgaatc cactaaatga atatggtcgt acgaaattat taggcgaaaa agcagtagaa
    25501 gagattttag aagactatta tatcatacga acttcttggg ttttcgggat ctatggacac
    25561 aatttcgtct tcacgatgca acgcctagcg gaaacccgcg acaaactgac tgtagtaaat
    25621 gaccagtttg ggcgaccaac ttggacacgt actttggcag aatttatggt atatgtcatc
    25681 gaagaaaaag caccatttgg tatttatcat ttgtcgaacg aaaatagctg ttcttggtat
    25741 gagttcgcaa aagaaatatt gaaagataca gatgtagaag ttgcacctgt tacgtcagaa
    25801 gaatttccgc aaaaagcaac acgtccgcag tattcagtca tgagcctgaa aaagacagaa
    25861 gcgttaggtt ttgtcatacc aacttggcaa gaagcacttg cgcaaatgct ggaagctgcc
    25921 aaaaaaatga ataagtaatt tcctttttat caaatagaat ttggagcatg atatggaaaa
    25981 acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg aatcaggcag attcgaattt
    26041 ggtgcttgac ctgtttctaa aactccgcta tcttattggc gttttagtca tcgtggtcat
    26101 cgttactttt aatttaaacg gaagttcact tggtgtttgg gataaatatg tgtcgcaaag
    26161 agacgatggg aaaaaaactg atgtgatttt tggacagaat cgggaagtgc ggtcagacga
    26221 atggctggtg cagacacctt tttacctctc acaagctgaa gaggactttt cgttggtgaa
    26281 cgaggattat tcattaaacg gacaaaacat gatcattgcg tataattctc ctgtaaagga
    26341 tattacggtt atcggaaaac cattcaactg gggattcttt ttcctcggaa gagatcgagg
    26401 actttccttt tattgggcga tgaaattagt cgtcatggtc ttgcttggat tcgaagtact
    26461 gatgatatta acgaagagga aaaaagctct atcgctaatc ggtgcatttg cgctgacatt
    26521 ttctccagca gtccagtggt ggttcatgca acatgtgggc gatttgattt tctttacttt
    26581 gggactgatg attgcttttt atcattattt ctacaatcat gataaaaaat ggctacgaac
    26641 gttgatgatg cttttggtgg ttattttcgg tattgggttt attttagtga tttacccagc
    26701 ccatcaagtg atgctggcgt acttgctgat tttttatttt gttggcctgc ttatttacta
    26761 tagcaagaaa gtttcatggg atctgttgga tgtagtgttg attggagggg cagttttatt
    26821 tattggcgga gtcatggttc atttctggct aacgtcaaaa gacgcattgt tagcttcttt
    26881 gaatacctta tatccaggaa aacgggtaag tactggagga aagtggacga ttggcgaatt
    26941 tttttctttc cttacaaatt ggaaaattcc ttttgaagat atcaactatt cgaataactc
    27001 tgaagtggcc ctgttctatc acttttttcc aactgtattc ctagcaagtc catttgtgtt
    27061 atttggcaaa aaagatagcg aacaaaagtt actgggacgt attttgatga ttttttgtct
    27121 gttttctatc ttttggatta ccgttgggtt gccaaaatgg ctagcagaaa tcactctgct
    27181 gtcttatgtt cctccattaa gagccatcct tacttttagt tttgcggctt gtttattatc
    27241 gatctggttt atcgcttata tttggagaga agatgttatt cctatttggt ataaattcat
    27301 tttgttggca gtaaatgcat ggtcttattt ctatgcgatt acgcattcga aaatggataa
    27361 ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg ggaacagtga ttttagcttt
    27421 tgcattattt ggctggagac gcttgtttta cctcgctttt gtcgccttag ttatcgtatc
    27481 tggctttttc gtcaatccag tcgtagaagg tacaggagcg atttttgaaa aaacactggc
    27541 aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa gtttggttgt ctgaagatga
    27601 attatataac ttcaccccaa cattgggagt gaaatccttc aatacagtac gcttttatcc
    27661 agatatagct gcatggaaga aaatcgatcc gaaagaagaa tacgagaagt attataatcg
    27721 gtatgctcat acaagagctt ttatcgctaa ggaagataca aagttcattt tagatcgtcc
    27781 agatatgttt tcggtcacgt tgaattttga agacgcagaa aaattaggga tcgactatgt
    27841 gatcagtaaa cgaccattaa ctgattacaa ccaattatat gaagcgcagt tcacgcagct
    27901 atatggaccg gataaagacg gttatatgat ttttaaagta gaatatccgg attatccaga
    27961 tgtaccacaa acacaaactc aggaaaatca gctagtcgta cagtaatagg ctagctgaga
    28021 ataatcctga aatattagta gaaaggagag tgaagataat tcaagcaatc gtacaaaaat
    28081 ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttgct gacgctagtc ttttttgttt
    28141 ggacagcagg ggtaggtttt ccagcaactt tgtttatata taacaatagt tcattactta
    28201 tcgcttttag tgtggctatt ttgctcattt taaatgttta taaagcaaaa ggcgtcgatt
    28261 ttctttatct aggtgttgcg cttgcccttt tcattttctt ttcttacttt cgggaattaa
    28321 gaaatgagtc tttatttgga gacttactgt ttcctttgat tattttattt tgtttagtaa
    28381 ttaaatggat cgagtggaca aaatgggatc gcggtattta tctgattgtc tttacggtct
    28441 tcctcggaat cacgttatat cgtgtattta cagagatcca agtaccagaa ggacaaagca
    28501 tctgggctcc aggcaattcg ctttctgata tttggatcaa tacgaataca atcggaagtt
    28561 ctttgatgac tcttgcttta ttgatcagtg gatttgccag ttcttttgaa cgatggtatg
    28621 ttcgaatcat cggtgggata gcagtcgttg cagccttttt aggtatttgg gtctgccaat
    28681 ctagaggtgc attagtcgcg ttgattgttt ttgcgatatt ggatctttta cctaaatcgt
    28741 tgatgcgtgt cattcgtgcg ccatttattg cctacacggt tacgattctt ttagctttgc
    28801 cgatttctta tctggcggct gtctctgaaa aagtcaattt gtttaccggt cgagaagata
    28861 tttggcataa attctatcag acgctaggag aaaaatctga acaagtttta ctaggaatga
    28921 aaacatttat cttccagcga ggaaaccaat ttttaggaaa ccacaatagt tataattcta
    28981 ttttgaatat ctatggattg ataggatttg ggattgctgc tttgctgctt atccttttta
    29041 ttggtcgctt aacgttgaaa gcagatttat ccaacgggca gatgactttt atctgggcct
    29101 tttttgcggt gatgatgcag tcatttatgg aggatacatt gacttcatac cgatgggttc
    29161 cgattattta tatcctgttg gctatggccg ttcatcgata tgatgaacca gaagttacgg
    29221 aggaattcta tgttatggaa gaggcatatg acgatatgac ttatgatgat acatcttatg
    29281 aggaacaacc agatagcgct cattcttcca ggatccaacg ctatcattga taggcacaaa
    29341 atacgagaga ggctgtgaca accgtcgcag ccttttttgg tttattgcaa ttgtctagca
    29401 tcttctgtaa aatagaaacg ttagagaaaa tcttgtatca tagaaaatta agaaagaaat
    29461 aagaacgcag ctcggtacca aaaatatggt aaaatggcac ttgactgtaa ttcactgaaa
    29521 atcgcttgga ggaacacaaa tgtttaaaga tttgtattta tttatgaagg acatttatca
    29581 gaacagaaaa ttactgctac agttttcgat caatgacttc aaatcaagat atgcaggatc
    29641 gtttttagga atcttatggg cgtttatcaa tccgctattt actgtattga tttattggct
    29701 ggtatttggt gttggactga aagcacaaat gatcgatgca aaatacccat ttatcgtata
    29761 tttagttacc gggatgattc cctggttctt cttctcagat gcatttgcat caactacggt
    29821 tgtttttcgg gaatatactt atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc
    29881 cacgacgaaa atcctatcca atctgtacac ccatttattc ttcattctga ttggcatagc
    29941 tgtcgcgctt ataaatggta tatatccttc agcaatgtct ttacagctga tttattattt
    30001 attttgcatg tgtgctttct tgacaggatt gacttggctg acggcatcga tccaaccatt
    30061 tttacctgat atcatgcaat taattacgat cgtgctgcag ttgatcatgt ggacattacc
    30121 gatattatgg agccctaatc agtttgattc aggtcttgta agcatattga aattaaatcc
    30181 gctgtactat gttgttcaag gttatcgtga gtcattttta agtgaagctt ggttctggga
    30241 gcatggaaca tatactttgt atttctgggt tttcacgtta gtaatgttcg taatcggctc
    30301 tgcagtgttc cgtagattga aaccacattt ttcagatgtg ctgtaaggag aatagctatg
    30361 acagaatatg caattagact aaaagatatc acaaaatctt ataatatgta cgcaaaacct
    30421 tcggatcgat ttcgtgaagc cctcaatcct tttaagaaat cctatcatga tgtattttat
    30481 gcgttgaaaa acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa
    30541 aacggatcgg gaaaatctac aatgttgaaa atcattacgg gagtactgac accaacatcc
    30601 ggcacgatgg aaatcgaagg aaaaatctca gctttgctag aactaggatc aggattcaat
    30661 cctgagtatt ctggctatga aaatatctat ttaaatggaa tggttctagg attttcaaga
    30721 gaagaagtcg atgaaatggt cgatgatatc attgaatttg ctgacatcgg cgaccatatt
    30781 tatcaacctg taaaaaccta ttcaagtggg atgttcgttc gtctagcttt tgctgtagcg
    30841 atcaatgtga atcctgatat tttgattgtg gatgaagcat tggcagtagg agatcttgaa
    30901 tttcaactaa aatgtatgga aaaatttaca gaaatcaaga actcgggaaa gacgatttta
    30961 ttcgtttctc atgatattag ttcgattcgc cgtttttgtg atcgttccta ttggttgaaa
    31021 aatggaaaag ttgtcgaata tggtgatacg atggaagtaa cgacgaatta cgaaaacttt
    31081 ttgaagaaaa aatcagttaa aacagtagat cgaaataaaa accaagtaga acgcttcgca
    31141 gctccagata tcgtagacgt gacgaaagcc gaattgttgg ataaagataa aaatccagtg
    31201 gatatgctgg aacaaggaga aaaactatac gttaaagtaa cctatgaagt aaaagacgat
    31261 acaatcaaaa atccagtttt aggaattgct ctgcgtacag tcgacaattt ttatgtgtgc
    31321 ggactcaata ctctactaga tggtatcaag atcccttgga aaaaaggtga gaatatcttt
    31381 tatctggagt atgaaaagtt aggtttgtta gctggtgaat attattttga cgttgcggtc
    31441 tttgaagaaa atgcaacggt tcctttggta tacaagacaa aatacatgaa cttgtttgtc
    31501 agcggttctt atatcggaga aggcatcgtg gtcttagacc ataaatggga agaagggact
    31561 catagcaatg aaatatgatt ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat
    31621 tgccgcacag atcaaaccag gaagtaaagt tttagagttt ggtcctggaa acggccgatt
    31681 aacgaaacat ttgatcggag caaaacaatg tgaagtgagc attgtcgagc tagataagga
    31741 attgttcgat tttgtcagcg agtttgccca agatggattt tatggagata tcgaaagttt
    31801 tgaatgggca aactattatg ctggacaaac atttgattac gttttgtttg ccgatgtttt
    31861 agaacatttg gtagatcctg gaaaaacgtt gaagaaagtt agagaatttt tgaatgaaga
    31921 aggagaaatt ctaattacat tccctaacct tgcgcataat tctgtcatga ttgatttgtt
    31981 caataatcaa ttgccatggg cttcttatgg tttgctagat gaaacccata attcttttta
    32041 tactcatgat ggcttccaaa aagtttttga aaaagcagga ttatacatca atattgaaga
    32101 ttatttgtac ttagctgtag gtgatacaga attaaaatca acttatgaag aattgccgga
    32161 agctgtacgt tatgatttca aaatgcgacc atttggcgaa gtctaccaat acttcttctc
    32221 attgatgaaa catccagtag cacaaagttc cattgcgcag ccgcaaaatt ctaattatgt
    32281 gaaagtatta gaagtaatcc agcaaacgaa acaagatgaa ataattcaac aaataccatt
    32341 caataatttc acaggggaaa atgagacact aagtttccat gtatcagaat tagcagaacg
    32401 aatggtgttt cgcttcgcgc agcaacctag ctttatcgaa ttcagcgcag aagctgctgg
    32461 agaaaaactc acatttatcg attcaaatgc tgtagtcaaa acagtaaatg attgttattt
    32521 gtttgatggt aaagatttac cagagttctt tttaacggat attgcaggga aagaagtgac
    32581 tatccattgt cactatcgtt ttatcggaga attgacacca acaatgaaag aattgctgga
    32641 aacaatccgt ccaatggctg aagttacaaa gcagctttct gagaaaaatg atgaattaga
    32701 aagagaaaat cattatctta ttgagaataa tacaagatta gatcagacgt tgaaaacaac
    32761 cataaatcgc tattgcacgt tgttggaaag tgatgaatgg acaatcaaac atagattagg
    32821 gcgacgtaag aaagaaacat cgaaaaaaat ccaagaaaaa gagctttctc tatgtattga
    32881 cgaaaaaatc tgggatgcag aaacaaagat attgaaaatc atcgggtggg gaatcgctaa
    32941 ttcggatcgc caaccattgt cctataagtt atcagccgac caatcaccat tttttgaagc
    33001 aattcctgtt ttacgagaag aagtcaatca agcaaaacag ctagctgaag gagcaaaagc
    33061 aggatttgaa ctgcggatcc tttgcgagaa ggaacgttca ttcttagttg aagcagtggc
    33121 tcaaaatggt caatcatgga tcattgaaat gtaaataatg attaacttat aaagataagg
    33181 agtaaacgaa tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa
    33241 cgggccactg gcgatttgct tattgtgggc tgggcaattg atgaagtgac caaagaaatc
    33301 cctacgataa aggtagaaaa agaaaatgtg attgctgaag caacacatgt cgtaagattg
    33361 gatatcaatc atttatataa tcttgatgtt aagacccaaa gcggttttaa ggtacgttta
    33421 tcagggaaaa tgagaggaaa agcaatctta gacttccaaa cagcaagaca tcaaaatggc
    33481 atagctgtga aattaaacgg taaatatcct tatgatgacg gcattgaatc ttcatgggaa
    33541 agaaaaaaaa gattactgaa aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa
    33601 gcaatccgcc gggtcaaact ggaattgaaa ccaggatcga tcgactatgc agagtggatc
    33661 agtcgtcatg agaaacctga tctgaaagag caaaggaaag aagtacaagg atttgcctac
    33721 cgcccattga tttccatact tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa
    33781 tgcattgatt cggtacttga tcaaacgtat gatcactggg aactatgtat cagtgatgat
    33841 gcttctactg atccagctat ccgaaagtgt ctagaatcct atcaagcaaa agatgataga
    33901 attaaagtcg ttttccgaca agaaaatgga cacatcagtc tggcaactaa ttctgcattg
    33961 gaaatggcag aaggagagtt tatagcgctc ttagataatg atgacgaatt acctccattt
    34021 gccttatatg aagtggcaaa agtgctaaac gtacatccag aactggattt gatctacagt
    34081 gacgaagata aaatcgatgc agatgggaat cgctttgacc cgcacttcaa agcagactgg
    34141 tctcccgata cattgatggg aaataattat atctctcact taggcgtata tcggacaagc
    34201 attgttaagg aacttggcgg tttccgtaaa ggatatgaag gctctcaaga ttatgatttg
    34261 gttcttcgtg tgacagaaca aattcctgca gaacacattt accacattga ccgtgtcttg
    34321 tatcattggc ggacgatccc gggatcaact gcaagcaatg gagaagctaa aagctacatc
    34381 tatgattctg gagtgaaagc attgacagat gctctatcta gaagaaatat caagggaagc
    34441 gtacatcctg gcagaatttc tggtttttat gaaatcactt atgatgtttt gcaagaagac
    34501 ttggtcagtg tgatcattcc aactaagaat ggctatgaag atctaaaaac ttgtgtggat
    34561 tcgatcatcg aaaaaacgtc ttatccaaat tatgaaatca ttattgcaga taatggaagt
    34621 acagatccta aaatgcaaga attatttgca gaatacaagc atcagctgaa agaccgattt
    34681 atcgtcgaat taattgacat tccattcaat tattcacgta tcaacaatct ggcagcagaa
    34741 aaagcaaacg gcaaatactt tttattcttg aataacgata cagaagtgat tgaacctgac
    34801 tggatgacag cgatggtttc ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa
    34861 ttattttatc cggatgatac aacgcagcat gcaggtgtat tattagggat cggtggcgtt
    34921 gcaggacatg cattgaataa ctatgatcga acacattgtg gttactttgg ccgattggtc
    34981 attgacgtca attacttagc agtcactgca gcttgtatga tggtcaaggc agctgacttc
    35041 aatgctgtca atggatttga tgaaatatta gaagtagcat tcaacgatgt tgacttgtgc
    35101 ttgaaagttt atgaactggg tcgttacaat gtctatgcac atcaagcaga attgtaccat
    35161 ttcgaatcta aatcaagagg ttatgaagat acaccagaaa aacaaagacg ttttgctggg
    35221 gaaatcaaaa aaatgcaaga taagtggcct tcttatattg ctcatgaccc attttataac
    35281 gataatctaa ctaaagaagg aatcggcgat ttttcgttga gacctgattg attaaaagaa
    35341 ctgtattttc atttttatta atgaaaatac agttctttta atttaatt
//
LOCUS       DACIRY010000015        34783 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.015, whole genome shotgun sequence.
ACCESSION   DACIRY010000015 DACIRY010000000
VERSION     DACIRY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.015"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IYE22_000616"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IYE22_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ9505849.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3191)
                     /locus_tag="IYE22_000617"
     CDS             complement(2886..3191)
                     /locus_tag="IYE22_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505850.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3336..4541)
                     /locus_tag="IYE22_000618"
     CDS             complement(3336..4541)
                     /locus_tag="IYE22_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ9505851.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5235..5621
                     /locus_tag="IYE22_000619"
     CDS             5235..5621
                     /locus_tag="IYE22_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ9505852.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5719..5910
                     /locus_tag="IYE22_000620"
     CDS             5719..5910
                     /locus_tag="IYE22_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9505853.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6424..6756
                     /locus_tag="IYE22_000621"
     CDS             6424..6756
                     /locus_tag="IYE22_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9505854.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6666..6869
                     /locus_tag="IYE22_000622"
     CDS             6666..6869
                     /locus_tag="IYE22_000622"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9505855.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            7053..7208
                     /locus_tag="IYE22_000623"
     CDS             7053..7208
                     /locus_tag="IYE22_000623"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ9505856.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7484..8824
                     /locus_tag="IYE22_000624"
     CDS             7484..8824
                     /locus_tag="IYE22_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ9505857.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9065..9472)
                     /locus_tag="IYE22_000625"
     CDS             complement(9065..9472)
                     /locus_tag="IYE22_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ9505858.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(9661..10488)
                     /locus_tag="IYE22_000626"
     CDS             complement(9661..10488)
                     /locus_tag="IYE22_000626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAQ9505859.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            10797..11015
                     /locus_tag="IYE22_000627"
     CDS             10797..11015
                     /locus_tag="IYE22_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9505860.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            11036..11671
                     /locus_tag="IYE22_000628"
     CDS             11036..11671
                     /locus_tag="IYE22_000628"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ9505861.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11864..12103)
                     /locus_tag="IYE22_000629"
     CDS             complement(11864..12103)
                     /locus_tag="IYE22_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505862.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12127..13650)
                     /locus_tag="IYE22_000630"
     CDS             complement(12127..13650)
                     /locus_tag="IYE22_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ9505863.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(13668..13790)
                     /locus_tag="IYE22_000631"
     CDS             complement(13668..13790)
                     /locus_tag="IYE22_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ9505864.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(13820..14803)
                     /locus_tag="IYE22_000632"
     CDS             complement(13820..14803)
                     /locus_tag="IYE22_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ9505865.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14827..14976)
                     /gene="rpmG"
                     /locus_tag="IYE22_000633"
     CDS             complement(14827..14976)
                     /gene="rpmG"
                     /locus_tag="IYE22_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ9505866.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(14997..15374)
                     /locus_tag="IYE22_000634"
     CDS             complement(14997..15374)
                     /locus_tag="IYE22_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505867.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15406..15585)
                     /gene="rpmF"
                     /locus_tag="IYE22_000635"
     CDS             complement(15406..15585)
                     /gene="rpmF"
                     /locus_tag="IYE22_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ9505868.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15600..15869)
                     /gene="rpsN"
                     /locus_tag="IYE22_000636"
     CDS             complement(15600..15869)
                     /gene="rpsN"
                     /locus_tag="IYE22_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ9505869.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16392..18164)
                     /locus_tag="IYE22_000637"
     CDS             complement(16392..18164)
                     /locus_tag="IYE22_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505870.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18118..19872)
                     /locus_tag="IYE22_000638"
     CDS             complement(18118..19872)
                     /locus_tag="IYE22_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505871.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(19982..20551)
                     /locus_tag="IYE22_000639"
     CDS             complement(19982..20551)
                     /locus_tag="IYE22_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ9505872.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20569..21993)
                     /locus_tag="IYE22_000640"
     CDS             complement(20569..21993)
                     /locus_tag="IYE22_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ9505873.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(21995..22663)
                     /locus_tag="IYE22_000641"
     CDS             complement(21995..22663)
                     /locus_tag="IYE22_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ9505874.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22794..23378)
                     /locus_tag="IYE22_000642"
     CDS             complement(22794..23378)
                     /locus_tag="IYE22_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9505875.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23713..23943)
                     /locus_tag="IYE22_000643"
     CDS             complement(23713..23943)
                     /locus_tag="IYE22_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ9505876.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(23958..24908)
                     /locus_tag="IYE22_000644"
     CDS             complement(23958..24908)
                     /locus_tag="IYE22_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ9505877.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24905..25747)
                     /locus_tag="IYE22_000645"
     CDS             complement(24905..25747)
                     /locus_tag="IYE22_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ9505878.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(25880..26605)
                     /locus_tag="IYE22_000646"
     CDS             complement(25880..26605)
                     /locus_tag="IYE22_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505879.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(27066..27380)
                     /locus_tag="IYE22_000647"
     CDS             complement(27066..27380)
                     /locus_tag="IYE22_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ9505880.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(27396..28991)
                     /locus_tag="IYE22_000648"
     CDS             complement(27396..28991)
                     /locus_tag="IYE22_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAQ9505881.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(29014..29904)
                     /gene="iolE"
                     /locus_tag="IYE22_000649"
     CDS             complement(29014..29904)
                     /gene="iolE"
                     /locus_tag="IYE22_000649"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAQ9505882.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(29921..30946)
                     /gene="iolG"
                     /locus_tag="IYE22_000650"
     CDS             complement(29921..30946)
                     /gene="iolG"
                     /locus_tag="IYE22_000650"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAQ9505883.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(30970..31977)
                     /locus_tag="IYE22_000651"
     CDS             complement(30970..31977)
                     /locus_tag="IYE22_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9505884.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(32090..33250)
                     /locus_tag="IYE22_000652"
     CDS             complement(32090..33250)
                     /locus_tag="IYE22_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ9505885.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(33326..34780)
                     /locus_tag="IYE22_000653"
     CDS             complement(33326..34780)
                     /locus_tag="IYE22_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAQ9505886.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT        10889 a         6932 c         5320 g        11642 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagta tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctaaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcaataagtt tggtaagatc attatcagta attgaaccta
     1141 caccataatt ttcatgatct ttgcgccgtt caattgctaa atcagccact tttttccctg
     1201 attcaaaaga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagctga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggttcc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta agctcttttc aaacgtaatt accccattag
     1681 gagcagtttg tgtatcagtt gtttcagccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggg
     2101 tgcttggaga aatttcatct tgtgcttgaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaaccaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa tagttatttt cttagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa acttatgtta actagtttac ttaaagaatt tcatgcacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ctctttctta gattaaactt cagagtattc
     2581 aaatagcgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcattcctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt attaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgcttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3301 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattatcc
     3361 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3421 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3481 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3541 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3601 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3661 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3721 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3781 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3841 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3901 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     3961 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4021 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagatttcct
     4081 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4141 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4201 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4261 aaatctttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4321 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4381 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4441 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4501 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4561 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4621 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4681 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4741 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4801 aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt tcgttcaaat
     4861 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4921 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     4981 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5041 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5101 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5161 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5221 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5281 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5341 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5401 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5461 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttaata
     5521 aaactgataa agaaattgga aaggttctgg gtattacaag acagggtgcg acaaatttaa
     5581 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5641 tttgtatgct tctttgctcg tcctcctcct tccccaaaaa ataaatagat tataagttca
     5701 tgggaagaag gtaaatttat gaatacttta aaagaagatt tcattttagc aaagaatgga
     5761 aatgaagaag cagttgtggc cattctcaaa agatttagcg ctttggtcca taagcaatcc
     5821 tggagaactg gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta
     5881 gctatctcga aattcgaaat taaagaataa ctcaactaag ggagtctgtg acataagtaa
     5941 aaagatagtt tcaaatttcg aacaataaat ttgaagccat cttctttata attataaagt
     6001 atcgatttta cagcgttcta taatgattca tagaatctaa aacgaatgat tttaattatt
     6061 aacgtttttt aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa
     6121 ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc agaactgggc catggtactt
     6181 caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta
     6241 gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat
     6301 tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac
     6361 taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact
     6421 ctattgaaaa aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag
     6481 aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc
     6541 cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga
     6601 ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa
     6661 gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt
     6721 acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt
     6781 tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag
     6841 aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa ccaaattgat gctgtttggg
     6901 tgacggatat tacttacatt ccatgtaatg acggtcgcct ctatttgtcc acctatattg
     6961 acttagcaac tcgtattcta cgttactata tggtggattc acacatgaaa aaagagattg
     7021 tcgtccgtcc attacaaatt tattaaggag aattgcctac ggtaattcat tccgatagag
     7081 gtagccagca tcggtctttt atttatcaag agctgttgac cgaccaccac atcacacatt
     7141 caatgagcca aactgggact cctgttgaca atgcagtaat tgaagcgttt catcgatcaa
     7201 tcaaatgaga attaatcgac tccaacaaat ataaaagtaa aatagagatg aaggtgttaa
     7261 tccaagatta tttagaagac tactacccaa acaaaagaat ccatacaaaa tttatgatga
     7321 ctccttgaca atttgaagcg aagaaaataa gggatctata atatctagtg ttggtgacgc
     7381 cttctgttgc caatactttt tcttttttct aataaacgca aacagacacc ttgaaatcct
     7441 ttagaataaa gtcgacgaaa accattcaaa ggagtgtttc aagatgtcct attcagagtt
     7501 taccaaagag ttactagata ttctagacct aaatcttacc tttcatgaag atgcctttcg
     7561 caaagagcgc attaatgacg aaacatgttt tgtttttgat ggcacattga cctatcaacc
     7621 agaggaatgc ttccattgcc actatcaaaa taagcaaacg atcattaaat ggggctggaa
     7681 aaaagtatcc atcttattga atgatgtcag taactacaag accattgtgc ggattaacaa
     7741 acagcgcttc aaatgtaaac actgtggaaa gactttttta gcagaagatt cagttagcga
     7801 tcgtcgctgc tccattgccc gcagagtcaa acaagccatt cttgagctat taagtgagcc
     7861 tatttctatg tctttgattg cacgtatgaa gcacatttct ccgaccactg tcattcgaat
     7921 acttcgcagc cttcggccta aaaccgtttc tttgaaccag ccgttaccag aagtggtgtg
     7981 tttcgatgaa ttcaaatcgg tgaaaaacgt ctcgggagcc atgagctttg tcatgatgga
     8041 cggcaagacc catcaactga ttgatattgt ggaaaatcgg caactcaatc ctttacgcga
     8101 ctatttcttg cgctacccta gaaaagtccg tgaacaagta cgtctagtgg tttcagattt
     8161 ctatacaccc tatcgcacat tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga
     8221 tcgctttcat attagccaac acatcggtcg ggcctttacc aaccaccgaa ttcaagtaat
     8281 gaagaccttt aaaaaagggg atcgtcggtc gaagcactta aagaaatact ggagactact
     8341 tcaaaagaat gcgtgggaat tgaatggtca gcatcgctac tggcgtccgt catttcgaga
     8401 tcatctgaca gaagcggaga tagtcgatcg gctattatat tatgatgact ccttaaagcg
     8461 aggctacgaa gtctaccagg tttttctttc cgctatcaga agacaagatg ttcctgaatt
     8521 cgtcgcgctc ctaaaagaag actacaagga gttgccagag cactatcaac cagtgttcac
     8581 gaccttcaag aaatatcgaa cagaaatcaa acgagcgttg cgagttccct attccaacgg
     8641 ccctatagaa tgtttgaaca accatatcaa agtactaaaa cgaattgcct atggctttag
     8701 aaactttcaa aactaccgag aacgaatctt tttatatcga ggaaaatatt tcaagaaaac
     8761 taagaacact acccaactga ctaaagccag aacaacaaca cggctagaca agatagctgt
     8821 ctagccgtgt gagtatctcg attattctat tgaatttctt caccaacact cattgacgaa
     8881 gagccaaaat aagtaatcta accgactgtt aactaaacca ttgcaggtcc cgttaaagat
     8941 acttgtgcct gttgtcaccg cttctttttc ttgtccacaa gcgcttaaca cacttaataa
     9001 agcccctgct attattaata ataatatctt tttcttatta aaaacctcct catctatttc
     9061 cagattagta aatgtagtaa tctttgcttt cttttttctc aatactaata actaacttat
     9121 ggggtaaaca aatactcgtt tgaccgggtt ttgagatcca tccagttcta accgcgattt
     9181 gatcaggact attatcttct ttaactcgaa ttcttccatc tttaatctca acaatattat
     9241 attgtccctt cgcaggatga taattcacga gtttatgatt gatcttatcc aaatcgaagc
     9301 gatccatttc cttgccatca atttgaacga tggcatattt gatagcatct tcagttgttt
     9361 cattctcctt ttctatagag aatacaaata agggaataaa agagggaata atcaaaatgc
     9421 caataattat gacatcgcct atcttgacac cagcttttct taaaaatttc attaacgata
     9481 tctggctaaa catattagaa tttttccatg gaaatggtga aatttttcgg aaattatctg
     9541 cattaagaga atcagttaaa caaccctcaa gttgttcctt aatacattgt tgatccatct
     9601 tcataccaat aaaaactagt ttattaatgc gttcaccaaa caacggatgc caggcttcct
     9661 tcaattcggg tttttttgta taatcatttg acactcaatt ttaggtaaac tagctaccca
     9721 ataatttaat gattcaatac ttttatggct acccgatgtt tcaaagatcg tcgaaaaatc
     9781 actatgttgg gcaaaccaaa gaaacccttt tgctcttgta atattgccag gaaaattttt
     9841 atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac cgatttcgat agataaaact
     9901 tgagatacca tattcttctg tttcaggaat atgattttga tagccaagct ctaactcttt
     9961 gaaccagccc attgaatagg aagtctcatc tatattaaaa agatttgtat ttagaatttt
    10021 cgataagtct acttcacctt taattgtcct aattattttg gcattggact gtaacttagc
    10081 aattagaccg ttaattttgt aaatcgtctt ggttgacact aagtcactat tatttaaaag
    10141 taatatgtta caacactcga attgatcaat taacaagcca cgaatatcct gttcttcttc
    10201 aactacatta ccttcagctt ttctttcctg aatcttttca cccgtttcga aattatccca
    10261 aaaacgtttt acatccacta ctataataat ggcaccaatc tctaatcgtt gggtcaaatc
    10321 aatgtctaaa gggtttttag ctaatataat tgcttgaata attggaattg gtttgctgat
    10381 tcctgaagct tcaataacaa tttgattcaa gtcagaaaat tgcactaatt gatctagttc
    10441 aacaatcaaa tcttcgcgta atgtacaaca aatgcatcca ttcggcatcg aaacaagttt
    10501 ttctccagtt cttttaaaac ctttcttgtc gactaatacc ccatcaacat ttacttcccc
    10561 tatgtcattt acaatgacag caatttcctt tccgtgatca ccgtacagaa tatgatttag
    10621 aagtatcgtt tttcctgaac caagatagcc agttaagatt gtaataggta ttttttcata
    10681 gggaacctct ttctatacct tgaatttaca ctttaagtgc aacacataaa aatgagtcat
    10741 aatcgatatc caactagtat aagtttgtcg agaacactcc tagaaggaaa ataattatga
    10801 gccagtattg tcatcttact atactcaaac gagaaagaat ctttctgctt cacgaagaag
    10861 gattttctat tagtataatt ggcaacgaca ttcagcatag cccgtttact attttacatg
    10921 aactgagaag aaacaagaaa aaaactaatc accttccaaa gcggaaaaaa attatgctta
    10981 tcgaagaaaa aaatgcggac gttatcacat tctaaatgat ccgtgagttt ggtcaattgt
    11041 tcgtcattta tttgtcgatt ttcagtggtc gccagagaaa atctcgaatc gtttgaagca
    11101 tgaacatatg gacttttcaa ttagttttat cacgatctac cgtgcaattt gcaatgatct
    11161 tttcgataca gaacccctct cacacggcaa taaaggcctg atccgttcat tgcaccatcg
    11221 aggaaaaaca agacatacaa aaaatcatgt cgagtctcgt gggaagattt gccttactca
    11281 ttcgatccat ggccatccaa aatccgcaga tgatagaagt gattttggtc attggaaagc
    11341 cgatattgta gctggcaaaa ctagtggtac ttgtttagtg actttgacag acagaaaatc
    11401 atgcttcctt ttagcaggaa agaaggtccc cagaaagaaa gccgcttatg tgagcgagaa
    11461 aatgattgaa cttttaagca gtattccaaa tgaggcggtc aaaacgatca ctccagatag
    11521 aggaaaagaa ttctccaatc atttcgcagt tacagaagta ttgaataata tgccctttta
    11581 ttttccagat cctcatgccc ttggcaacaa tgaacaaatt aaaatacgaa tagactcatt
    11641 cgagaatact tgccaaaatc gaaagagata gctacggtag ataactcaac gataaatcag
    11701 atgattgaaa aactgaataa cagacctaga aagtgtttag attggagaac accttatgaa
    11761 gtcttctttg ataaagtgtt gcgcttgatt tgacaattca agaaataata aaaagcagat
    11821 caaatccaaa taagggaaaa atgatctact ctggtattat caattaattg gccaagctat
    11881 tatgccgttt tgctaaacgt ctacttcttc cagtattgac cttgataaaa caacaatcag
    11941 gatcccgttt taatactcta tgaactgtgt caatactaac atgtaaagtt gcagcaatat
    12001 cagctaatgt actcccattt aatctcattt gctttgcaaa ttgataacgc cgataagcat
    12061 caatattctt ggaatccata gacaataata cttttcttac catgtatttc ctccaaagat
    12121 tggattttaa tgggccatca tttcttgagc aatctcatgc tcagataatg aagctggata
    12181 gaaagtaggc caattatgca tttcattata aagtttttct tgactttctc caccaaagaa
    12241 aatatggaaa tgctctgcct tacttggtgc aattccatga tcgctaaatt gaatatattt
    12301 aaaacgacca gcacctgcat catcagtttc aaaattaaaa cgaacaccac ggtttccttt
    12361 ttcataattc aaaattttgt atccaacata tttgtatgtg tattgatgat gttcaccgtt
    12421 gactacaaaa tcaatggtgt tgtctttaat gttgatagta tcaatatctg tcttatagcc
    12481 agtagtataa taatctttgt actcggcagc tgtcatgtct ttattgagtt ttgctttgta
    12541 atcgaaaact tcatccaaaa ttccatcttt taacaacgga taaacagatt gccattcgcc
    12601 tgtataatca ctcaattcgc gatccttcac atcggcatct ttaaagtacc cattagcaac
    12661 tgtcttttca tccttcgctt ctttttctgg aactacctct tcaccagcaa ccatagttgt
    12721 tttctctaac gcttttagat tgtcttccat tactgaaaca taatcttcac cattatccat
    12781 ttgttctttt gtaagacttt caagaggatt caatacttcc aattttatcc cagcttcatt
    12841 ggccaaagtt tttgcgattt tatcattagc attcttttca aagtaaatat aattgatctt
    12901 atttttttta acatactctt ttaattcagc taatcgacca gaagatggct cttcttcagg
    12961 acttagtcca gcaataggaa cttgtatcaa tccataatcc aaagctaaat atccaaaggc
    13021 tgcatgttgt gtcacaaaac ttttttgttt cgcttcttta agactagtgg tatagtcagc
    13081 atccaaccgt ttcagtttag tcaaatactt ttcagcattt gtctcaaata cttttgcttt
    13141 tttcggatac aatttaacta actgatcctt gatatttgaa acttccttaa ttgccatttt
    13201 gggtgaaacc cacgtatgag gatctagttc atgatggtga tcttcttctc cgtgatcatg
    13261 atcttcttct tcactacctg gcaataaaat catgtctttt gttccttcta ctacattagg
    13321 cccagctttc ttccaacttt ctttagcttt tggtacccat gattccatgt tttcgttatg
    13381 atatacaaaa acatcagcat catgagcttt tgccatatcc ttagctgagg gctcatagtc
    13441 atgaggttct gatcccgctg gaaccattaa atccacgttc gcttcatcgc cgacgatgtt
    13501 tttggtgaaa tcatacattg gatagaatgt tgttacaatt tctaattttc catctttatc
    13561 attcgatact tttgcatcat tagtgctgga gcaagctccc aataatccta gagctgctat
    13621 cccaataagt cctacactga ttttcttcat tctttttcct tccttaatta tgctaatttc
    13681 ttcattttat tttgtttatg ttctttgata atttttaatg ccctttttct agttttgata
    13741 ttcgggcttt taagtcgtcg atatgcacta gatatatccg ttttctccat tagaaattac
    13801 ccctaatcaa tacttatcat tatttgcttt ccgccagtaa tttatcaaaa ctctgtttga
    13861 tcctttcttc atccaacatt tttccaatta gaataatagt ggtttggtct ttttgtcgag
    13921 tggtgtattg aaaattaacc tgttcgttta ccccttgtat ggcaactata aaatcttgat
    13981 cttgcaaagc aatcaatcct ttaaaacgat acaggctccc ttggttggtt aaaatcagcc
    14041 ggtcaattaa ttggaataaa aaatcttttt ttatgtcttg atctgaagtt aagagaattg
    14101 atttaaactc atgttcttga tgatgttgat gatagtgttt gccattatag tgattttctg
    14161 atgaatcact ttcaaaaact aagggatgat tgaatttttc caaattataa aaatcctttt
    14221 tttgaatcgt ttcactatgt gtgaacattc gaatatcgga taaagggtta atacttttga
    14281 tttctctctg taattttttt agtgcagata aattcaagtt ttgttttcta gacacaaaaa
    14341 tgcgatctgc tatgacaagt tgcttaaggg cttgtggttc tctttcaata agttcaaaac
    14401 tgtcactatc aataacagtc aataaactat cgatatatac tcttcctctt agttgaggcg
    14461 ctgttaaaag tgtttgaatg attggttggg gatctgctat acctgtagtt tcaatgatga
    14521 tttgtgaaat tggttgttga tgttcttcta ttgtttccag taaagaaatc aatattgcta
    14581 ataaatcact gcgaagactg caacacatac atccattatt tagttgtaac acccgttcct
    14641 cgctatgaac taataatttg tgatcaatcc caacttcacc aaactcattt tcaattataa
    14701 taatttcttc cctggggtag ggtgagactt gcaaggcttg attgattagc gttgtttttc
    14761 cagcccctaa aaacccagaa acgattgtaa ctggtatgcc cattttgtca cctttttacc
    14821 acttagctat ttcacttctt tgaaaattgc ccgtctgcgc aattttggcg aatacttttt
    14881 taattctaac cgttcagggt tattccgttt atttttgctg gtgagataca gacgttctcc
    14941 agtttcgaca cattccaaaa taatattttg acgcataaat cttcctcctt caatacttat
    15001 cgatcttcaa gatatgcttg aatttgttgt gcatcttcaa aagttaactc gccagattcc
    15061 aattgtgaaa ctaaattttc tttcatatca acaggtatct cccaataacc atcaatacat
    15121 tgaaataact cctcgtcata ctcttcaaaa attggataga ttcttaaaaa atcatcaaat
    15181 gataagcctt ggtcctcata gtaatctttg actaatccat tttcaattgc ctgttgggtc
    15241 ggtaaacccg tttcagtaat aattcctctc tctaccatcg aacgataaaa agtgcattta
    15301 tctagcgatt gctgggtcgt ttcaagataa tactttaatc cttcttcaac taaaggtggg
    15361 taaataattt ccattttgat tccctcctaa aagttatata ttaaactatt ttgaagctcc
    15421 tttaattact ttctttccat tatagtaccc ttttaaagat acatgatggc tacgtctata
    15481 atcaccattc gcttcgtcaa atgaaatttc tggttttact acagtttgat gagttcttct
    15541 taaccgtttc ttcgccttag atgtttttct tgctggtact gccatgatat ttcctccttt
    15601 taccaacttg ctttttttac tccaggaatc aacccttgat gggcaagttc tctaaactta
    15661 atacgggaca taccgaactt tcgcatatac cctcttggac gaccatccgt ctgatcacga
    15721 agcctcagac gattcggatt tgaatctctt ggcaatttag cgagagcggt gcgatctcct
    15781 gcggctttta attcctgacg tttatctgcg tacttttcaa tcatcgccat ttgtttcttt
    15841 gctttagcaa ttttcgactt ttttgccata tacctttata ctccttttat tttgatttag
    15901 acatctcaaa tcgtaatcgt tacgttttga cactgatact ttaccaacaa gagttgctct
    15961 tgtcaacaat aaataacatg aaataaaact catatgaata gataactatt tgaaaacgaa
    16021 tttatcggta catagagata atcaaatcat ttaaaagaat atgcagttta agctctgaaa
    16081 aataagaatt cacttttatt agtccaatca aacacaaata ccgttttaag tagcttttcc
    16141 tctacttaaa acggcaatat gaaagaaact ataacttttt attcaatctt ttatgtttaa
    16201 ctggtttatc gccaattttt taaaactttg attattcctt atattataag aatagacatt
    16261 aagtgaacta attcaatcat actgattctt tcgtgtattc aaaattagat gctgtttaat
    16321 aaaattattc tttttgaaaa agaattataa ctatatccca aaaattgtag gttaatttaa
    16381 agcctatttc atcatcgtga ttgagcttct ttttcataga cgaatttctc agatagttta
    16441 gttttcttta tgagattttt gtatatttct gaccttcgat acaactcact atgagtacct
    16501 tgatcaacta tttttccttg atccatcact acaattttat ttaccttttc aatagacttc
    16561 aagcgatgcg atataattag tactgtctta tcttgtatta aacgatttaa actttcctga
    16621 atacgttttt cattttccgc atctaaactg gcagtaattt catctaaaat taaaattggc
    16681 gcatttttca aaaaagcccg tgctatggat aggcgttgtc gctctcctcc agaaagtgaa
    16741 cttccgtttt ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg
    16801 cagttagcta aacgagctgc ttttttcact tcctcatctg acgcttgact atttccaata
    16861 cgtatatttt ccaaaatact tgtattaaac aaagttacct cttgaaaaac aatagcgatt
    16921 ttgctaaaca aagaagctgg agatacacgt ttaatatcat acccatcaat taaaatacag
    16981 ccttcatcat aatcatacag tctagacact aatttcaaga tactagtttt cccggaacca
    17041 ctagcaccta ctaacgcagt cacttctcct tgtttagccg taaaagagat atgatctaaa
    17101 attggggtat tgttatcata tgagaaagag acatctcgca actcaatgtc aaaagatttc
    17161 agaggactat ctgatccctc ttggatactg gtttccttca tagctctaat tcgctgtatt
    17221 ttaggagcta aataaaatat ttcaagcact gcttctttca tagaatcgaa ggaatccttt
    17281 atttttattg cggctagtaa gtaaccaacg acgtagagta tagtcacttc acccgttaac
    17341 agtaagtgaa cacctactag aatgactact gctaaactaa catatgagaa aatagaggat
    17401 aaggccatga ctgaaaaagt acttaattct acctttaaat gaatcctttc actctcttcc
    17461 atttttttga aaagtctgtc ttgaactttt ttagatagat taaagctatt aatctcttgc
    17521 tgcaattcta tagtttcttg aaattcttcc gagttttctc tcaaagtatc gtaatattta
    17581 gtattggctt tcgtttggga tttctttgat aacaagatta agacaaaact aaataacgtt
    17641 ggcaaaataa cagctaatcc cattttgaca ttaccaacca aaagcatcac tgaaataaaa
    17701 gggaaaaaca gagccatacc gccggcttta ggtattgcat gactcatcgc atgctcaata
    17761 ccttcaacgt cagacatgat agtttgagat aaatctgata aattatgttt tgaaaaatag
    17821 gataatggta aatttgacaa atcgtctgca atttgcattc ttaaatgcgc actttcttta
    17881 taagtactat tatataagtt ttcatactcc ctatctaaca accaatacat gaaaagcaaa
    17941 gtaacgaacg atactgtaaa ataaagccaa ggcggcttta cattttctaa aactaattcg
    18001 tctatcagca tcattataat gaatgctggt accatattaa tgcagtatac aaaaaagcta
    18061 gaaatagatg ctttaaccaa atcttctgcg ccctgattag ttaaagcgaa tttgttttta
    18121 taaaaacttt tcattgtcaa gcctccaatc atttgcattc tcataattag tgaccaattg
    18181 tttatacact tgatgtttag ccattaattc attatgattt ccacgttcta ctattttccc
    18241 ttctgacaag acaagaattt catctacaga tttaatgctg gataatcgat gagctatcat
    18301 aataactgtt ttttctttca tcagatgttg aaaagccttt tgtagttcat actcattatc
    18361 tggatcaata gatgcactgg cttcatccat aataacaatc tttgcatctt ttaaaattgc
    18421 tctggcaata gctatacgtt gtttttctcc tcctgacaga taaactcctt tactaccaat
    18481 taaggtatgc tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag
    18541 cgcgtcaaat actttacttg ctggcgcttg aggatttgct atagaaacgt tatcgtaaat
    18601 actctgattg aatagtttcg tatcttgaaa gacaaaagca attgtgttaa taattgtgtc
    18661 ttttgaatag tgatttaaag gcttaccacc aattttaatc tgtcccttat ccacattgta
    18721 gaaacctgaa attagtttag ctatcgtact ctttccgctt cctgattttc ctactaatgc
    18781 ataaattctt ttttgttcta aaactaagtt gaaattttct aaaacacact cttctttgta
    18841 tgaaaagctg acattttcaa atgcaatgtc acaattttta atctgtgtct cgttcccata
    18901 ttttagttgg tcttgcttca tttctaaata aagggagtct agtgtttcga ctgcatatct
    18961 tgcttcaaac aagtgcatac ttacatacat tattctcatg aaagaaacaa aaataattcc
    19021 acttaaaaaa aacaacatca acaagtcaac agctaatatc ttgggagtgg taagagaagt
    19081 tataaaaaag gttataggaa ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa
    19141 aagaacaaaa ggtgttttcc cactcaaaga atacttatat gcaaacttag cataatcgtt
    19201 aattgcttta ttcaaggctt taaatgatgt tactttagca ccaaatatct ttataacttg
    19261 aattcctcgg atatattcta ccgtttcaga gcttaacaca tctaacgatt cttgatattt
    19321 ttgaataaaa tttgtattct ctgtcatttt ctttaacaag aatacaccaa ttactaacat
    19381 ggcgattaga accaatccaa ttttaaaatt aatgaaaaag cctaaggcaa ctgataaaac
    19441 aggtgttact atggcttgag cactatctgg aatcaagtga gcaattgcca tatgtgtttt
    19501 agccgcgttg tcatcaattg tctttcgaat atatcccgaa gaatttaaat caaaaaaacg
    19561 aaaacttgct tctgttaaac cgctaattcc cttttttctc aaattagtct ctaagcgaaa
    19621 cccaaggata tgagaaaata agccagaaaa aatataaaat aaagctccta aagttaacca
    19681 agcaacagtt tgaaccgcat aactcttagc taaatattca tctcctacaa taattagaga
    19741 cttaagaaat ttgtaaatac tgtaatatcc atagactgtt aatacgacag aaaaatcaga
    19801 aaataaagtg gctaaccatc cgtagtattt cattttaggg acataactaa ataatttttt
    19861 gtatactttc attttttcct cccccgcatt caatttcttc tcttattcgt ttacaactgt
    19921 taatgctttc cgtaatagcg taaattagag atgtacaaac agatagaagg taaactacgt
    19981 tctatttttt gtattttttt aagacttttc ttccaatcaa tacaccaatc atagataaac
    20041 ttgctacaac taacactgaa atgattcccc atatacctac cgcatagtgg atcattgcat
    20101 ccattgttgc agcagattgt cctttatcta tccaatcttg acgaaaacga tctgtaaaga
    20161 accatatagg aatccactgt ccggcagcac tgcctaattg cattagagcc caagacgcaa
    20221 cttgaggaat tgctcgatca tatccaaata cagatagaat aatatcagct aagatcgcca
    20281 tactaatcga actgataaac caaggtaagt acgccccgcc cattaacgta aaaaataaca
    20341 ataaaacaat tgtgtaaata gtaaaaattc cttttctagg gtagttatag ctaatataaa
    20401 caaatatgat tccagataat aatccaaaaa tccctgggga tatgctgtga ccaaacgccc
    20461 caccaatact actaaccatt attgaaaaaa agtaaattac tagggaaata actgtagcta
    20521 aaccaataaa aatataatct tttgttttca tatatattct cctcaattct atttcgttat
    20581 gtcattaaaa atctgttcta tcaaagttga atcttctact ctaagtttta tatctttttt
    20641 tattccacca ttttttaaat aaataattct attgcatgtt ttataaagaa attccggatc
    20701 atgagtggct ataactactg cacccttttc ttttaagtct ttaataaggt cagcaatcct
    20761 ttccatatta atataatcca acccacttgt aggttcatca aatagaaata aattagtttt
    20821 tgacaatgaa gctatagaaa ccaataatct ttgtttttct ccaccggata aatcaaacgg
    20881 taattgatct ttcattttat aaagttccat tttttttaaa attttttctt gctgatcttt
    20941 actaactatt tcattcccca gggctaactc gctcctcact gattctgtga aaaattggta
    21001 atccatttct tgcattacta aaaacggcga tgacaaattt tttttatttt cgattatctt
    21061 tcctttggta gggcgaataa tcttcatgag tattcttagt aaggttgttt tccccacacc
    21121 gtttttccct accaaaccaa taacatctcc aactttgaca tcaaaattta tgtcttttag
    21181 aatattttca taagtaattt cttcaacttt taatacatct gcacttacct tttcgatata
    21241 gggaacttca atcgaactat taaaaatgtc aaatttcctt aatgcgttat tgttttcagt
    21301 ttttaacaac tgaatagttg tagtttttac agttctattt ttaacaaata gaacttgatc
    21361 aattaattct ttcaaatagt aaaaacgatg ttcagctata acaatagtaa atcctttatt
    21421 ttttaaatca caaagtaagt cagcaagttt cttagtcgct ttgaagtcca aatttgaaga
    21481 cggttcatcc aatataatta atttagagtt tagcgataaa ctacatccaa tagaaatttt
    21541 ctgccgttca ccacttgata gcgaaaaaat gtttttatct aatatatttg aaagatctaa
    21601 taattcagct aattcattga ttcttttatc catatgctct tttgtaaatc caaagttttc
    21661 catcgaaaat actaactcac tcgttgtatt tgttgtaaag aattgtcctc taggatcttg
    21721 acttacgtag ccaatatttt ttgatatact ttcttgtgtt aaggattgta gcgagttatt
    21781 ttcaaataaa atttccccaa ccaattttcc gtcataaagt cttggaataa gtccattcat
    21841 taactttaat agtgtggatt tcccactccc agaagaacca gtaatcaatg taagactacc
    21901 tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa
    21961 agaaacattg ttaaattcaa acaattcttt cacctcaaat caaaatactt aagaaaaaaa
    22021 tagtaacgga aaacaggatg atatagtcta ataagctcat gtttgtttta tagtaagaac
    22081 ttcttttcac aggagattct atcccctttg ttattgcatt agcggtcatt tcttcagcaa
    22141 tcatagttgc acgtataaga acgggaacta acagaaattc caaatattgc agaggctgaa
    22201 aaaaagacaa gcgaatgttt ctcattttta aagattcttt aatcattctt atttcagaac
    22261 gataagtcgg aaagaatctg atcactactg taatacttaa cattattttt ttcggtaaat
    22321 gtattttttc taaagaggat aataattcat tgctagtata gacactaacc aacaaccatg
    22381 agatcattac gaccggcaac attcgaaata caatatataa ggatgtccca aaaaatatag
    22441 taaaaaactc tatcttgata gcatcaataa ttttcattac taaaaacaga actgaataaa
    22501 tcaagatgaa ataaatacat gtctttattt tacgaaacat caatactaaa actgtagcaa
    22561 taatgaaaca ccccacttga aatatatttc cctttcctaa aaaaataatg accggaaaaa
    22621 aaatatttaa agtaatcaag gttaacggat tgatttttga cactctttca cctccaaaaa
    22681 ataagatcgc actgttatct taagctgata aaaatgcacc gttattcaga taacagtgcg
    22741 atcttatcaa aaagcataac tgagaacaat tctcaatgca agcttttttt taattaactc
    22801 agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa tagcatagtc
    22861 ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa tgagtaaatg
    22921 tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc gatagtcttt
    22981 aacttatcta gacaacagac tgtctttttt gtcacaaatg atgtaatttt ttcaacaata
    23041 tcttgatatt tggttccagc tgattggaat aaaaccaaat cgaatatacg tttattctca
    23101 aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact tttctctata
    23161 tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat attaataagc
    23221 gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt aaaatgttta
    23281 taaaaagcac ccgtagttac gcctgcggtc aagcacagtt gccttatctt agtttgctga
    23341 aatcctactt tttcaaaatg gcttagtcca ctttctatta atttttggtg agttgtaata
    23401 taatctctgg tttctatggg tacttcctcc taataagatg aattaactat attatcttac
    23461 acaggacttg aagtttcaat cgttaacgtc tattactgaa ctcattcctt agagctaaac
    23521 taatgagaaa gagcaaaata actgatacaa aaagcaatcc tacaagaatt aatagactca
    23581 tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc
    23641 tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat
    23701 aaagcctttt acttaaaacc tttttttgta atcgtcaata aattttttcc ctttatcagt
    23761 taattgaaat cctatatcta aatagtcctc atatctaagt ttagtcagca tctcacttac
    23821 agatgctgga ctaacactta gaacttttgc taaatcatta ttttttaatt tcttttttct
    23881 ttttgataat tcctgaattg ctagtaaata aattagtata gtagttgttt taatttgtac
    23941 catacctacc ctccaaatta tttcgaaagg ccttcagcaa ttttatccag gttccatttc
    24001 atcatcgcat agtaactatc accatcttgt cctttttctg caattgaatc agtaaaaatc
    24061 gttgaataga taggaatatt ggtatctttt gatactgttt tcattggtct atcgtccaca
    24121 ctgctttcta cgaataagga gggaactttt gttgtgcgta atttttcaac taagtgtttt
    24181 atttgatctg gtgttccttc ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca
    24241 ttatacgctt tcgagaaata tttaaagcat ccttcacttg taacaatcat ttttttatca
    24301 ttcggaattg aagcaaattt agatttagct tctttgtcta gagaatccaa tttttcaata
    24361 tacttatcta gattttcttt atagaatttt ttattatcag gatctttttc cgctaactgt
    24421 ttttcaatat ttttagcgta aataataccg ttttctaaat ttaaccaagc atggggatct
    24481 tccttccctt tctcactctg accctctaag taaataacat ctatgccatc acttgctgca
    24541 aaatagtctt tgttttcctc tttgttcgca ttttttacta attttgtaaa ccaagcattt
    24601 cctccagttt ccaagttaac accgttataa aaaatcaaat ctgcttttga agttttttga
    24661 acatcttcag gcaaaggttc atattcgtgg ggatctttcc caataggtac gatactgtgt
    24721 aaatcaattt tatcttttgc tatattttca gtaatatctg ctaaaatcga attagtaact
    24781 actaccttta atttttcgtt cgaagccact gtatctttgt ctttactatt tgttgaacat
    24841 gccccgaata taaaaagagc aatcaaaatc cccagacttc ccagtactgc tttccatttt
    24901 ctcatcataa ctttctctcc ttctttctta aaaatttttg ttttggtgag attagaaaac
    24961 taattacaaa taagaatgcc gctgttaaaa caatacttga tcctgctgct atgttaaaac
    25021 tataaccaat gaacaaacct agcaatgatg agcacgctcc gactgtcgcc gaaataaata
    25081 tcatagtttt taaactgttt gcatataaat atgctgtagc agctggtgta attaacattg
    25141 caacaattaa tatagttccc acactttgca tagctgttac tgctactaaa gttaatagaa
    25201 tcattaatag ataatgataa aaactgacat tcatccccat tgcttttgcc attaatggat
    25261 caaaagaagt gattaataat tctttaaaaa atagaacaac tgtaaccaac actaaaacag
    25321 aaacgccaat tgtaatccac ttatcaatat cttgtacagc taaaatattt ccaaataaga
    25381 tatgaaataa atccgttgag ctatttgcaa ctcctataag gataactcct aatgctaaaa
    25441 aagaactgaa agtaatacca attgctgtat ctccttttat tacactattg ctttttataa
    25501 aagtaataat gatagaagct aatagaccaa atacaatcgc accaataaaa aaattaatac
    25561 ctaaaatata agaaattgct actcctggca aaacagcatg agaaatagca tcacccataa
    25621 gagacattcc tcttaaaata ataaaacaac ctactgttcc agaaacaatc cctataacta
    25681 cagatgtaat tagagcattc tgtaaaaagt gaaagtcata cagaccatta ataaaatttt
    25741 gaagcatatt attccccttg tatattagag ttagggtttt aaccttccgg ttttgattgg
    25801 ataaccctat gaaccatcgt tattttaatt tggtattaac tcatttcttt tgagataata
    25861 tatttattgg attgagaggc taagccgttc ccccctctcc aataaatata gtatctccat
    25921 aggctttttt taaattctct tcattaaata cagattctac acttccgtga gcaattagat
    25981 ttcgattcaa aagaataata ttatcaaaat acttctttac cttgcttaaa tcatgatgca
    26041 caattagcac tgtttttccc tgatgtttca attcttttaa cgtatccata attattcgtt
    26101 cactaactaa gtctatccct acaaatggtt catctaaaaa tatgaaatcc gcattttgag
    26161 caagacatct tgctaaaagt acacgttgaa attgtcctcc agataattct cctatttgat
    26221 gattagaata ttttcccata tcgacctttt ccaaagcctt tgaaactctt tgccattctg
    26281 catttttaat tctttgaaaa actttcattc cggcatatgt tcccatagat acacattctt
    26341 ttacttttat tggaaaagta aaatcaatat cagctttttg ctctacatat gcaatcttca
    26401 ccagtttttt attgatatct tcattattaa tggagacctc tccctgatga gggataatat
    26461 ttaacattgc tttaagcaat gtagattttc cagcaccatt tggaccaata atacctgtta
    26521 aagcaccttc ttgtattttt aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa
    26581 cattaagctt tttcacttct aacataactt tctccttcta aatgataatg attatcacga
    26641 tcatatggta tacaaaaatt atattttagt caaccctaaa ttttataatt tagatttttt
    26701 tctgttgttg aagattagga tgtcaaaatt atttgaaact actcgtatag taaatagtaa
    26761 gtaattttca aaacacgaag aaaaacaaaa agaaaaaagg aaacgccgaa gcatttcctt
    26821 tcagtgattt aatgagaaca acacgatttg acttagagtc tcaaaaagtc gaataggtac
    26881 actcatttaa gtgtctccat tcgacttttt ggggatgagc tggctattga ggtccgctta
    26941 ccctattgaa taaatacaag attgggtatt tctaataagt ccgcccttca tattggggtg
    27001 atcggggcga acatgccatc ttttcatata ccacctcctc gacactcaat aatcaaagat
    27061 tctcgttaat cttcttcgat gcttgcaatg atgttttcaa tattcatata acgaaaatct
    27121 tcttgtttga tataaacgat ctgagcggta catttttcct ttttgatgat ttcttttact
    27181 ttttcctcag aacgtgcgac ctgcggcgtt aaaagtaaaa cgtcatagtc tttataattc
    27241 agtttttctt caacgtttcg gatcgttgca taatctatct catacgatag atttttggct
    27301 ctgatcgttt caagcatttt tgatacgagc agcgacgcac tgattccggt ctcgcaaata
    27361 atcaatagat ccttatgcat aataccctcc tgtttttagc ctgcaaagcc aattggtgaa
    27421 aatgccacat aagttaaaat aacaatgact accaatgcta tagcagtcac cttggtgtat
    27481 ttccaaggct cgatatcgac ctttgtatcg aatgagttca acacaaattc tccatcaggt
    27541 ttcaatttgg aaaccaccat caaaatgata atgtccagaa cgaataatgc cgtcaaaaca
    27601 taaaggaaat ggatattttg gaaaagcatt tttgatagtg catacaagac gatatgcacg
    27661 gcaatcgtcg tttttgcccc aaaagcagtt gctttttttg aataaaaggc aaagagaatg
    27721 atcacgagca gcggcatgtt gtatagtcca ttgaattctt gaacgaactg atacaatcca
    27781 gccggtgcaa aagaaatgaa tggcgctacg ataatggaga taatccccac aacgaccgtt
    27841 actgttttac cagcacgagc gatttgctta tcatttgctt tcttattgat aagcggttta
    27901 taaaaatcca gtgaaaacaa ggtcgatgtc gcgttcaaag ctcccgcaaa tgatgataag
    27961 atcgcgccaa agataacagc ggcgaaaata ccaaagatca ttttaggcaa tacttcactg
    28021 actaaaaaag gatacgcgtt atctggattt gacattaaac tatcgccaaa aatattgcga
    28081 gcgacgatcc ctggaaagac aaggaataac gcacctaaga ttttgaagac gccgactagg
    28141 aaagcgccct tttgtgcctc agctaaattc ttgccggaca atgctttttg gacgatcatt
    28201 tgattggtac accagaaata cagattgtta aacagcatcc ctgtaaatag tgtcggccat
    28261 ggaacaacag ccgagtcgat cgatcccaca gagttaagca gccatggcgt gtttaccaca
    28321 acgtgttcga tcccgccaac aaagctgccg tcaccaagct tcattaagcc taaaattggg
    28381 accagcagac cacaagccag cagaccgact ccataaatgg agtcactatt ggcacttaac
    28441 gacaagccgc cgatcagcag atagaggatg ccgacaatac caattgccat cgctacaatt
    28501 atgatcgcga cgatcggctg gacgcccagc agctcatcaa tatggaagat cttattgaac
    28561 accagcgatc cagaataaag aaccacgggt aagaaagaag tcatataggt cacaataaat
    28621 aagatcgaaa taattcgttt ggtaaccgta tcataacgaa tctcgatgaa atctgagatt
    28681 gtattgatgc cgtatttaaa atattttggc aagaatatca cagccaaagc tacgatcgca
    28741 atcgtagcag ttacttccca ggccattact tccatccctg catgataact ttgaccattt
    28801 tgtccgacta tttgttcggt ggacaggttc gtcatgatga tcgtgccggc gattggcaaa
    28861 gcagttaagc tccgccctcc catgaagaac ccttctgatc cagaagtatc gacacttctg
    28921 gatctagaat aggcaaataa ccatacacag atgacaatca ctaggaaact gattatagaa
    28981 aaaagattca tcgtctagct ccttttcaat agattaaaga ccggtgacct ctcgaatgta
    29041 gcttctggct ttgactgcat attcaaatgg attggcttta gccgggtcct gttctgcttc
    29101 aactacgatc cagccttcat agtcgctggc atggattgct gtccagattg gagtgaaatc
    29161 aatcatccca tcaccaggaa ccgtgaagac gcctttcttc acgccgttta agaagctgtc
    29221 tttttccttt tctacttctt cagccgtttc ttgacgaata tctttgaaat ggatgtgttt
    29281 gatacgatct tgatgacggt tgtaaaccgc gattggattt tcaccagaaa agaccaaatg
    29341 tcccgtatcg aacaataatt taactttgtc tggatcagtc atttccatta aacgatcgat
    29401 ttcttccgtc gtttgaacgc cggtacccat gtgatgatgg taaacgatct ccatatcctt
    29461 ttcgtgggcc aaatccccta atttttctaa tccttccgcc agttttttcc actcttcatc
    29521 agtgaaaact ggtttatcat caaagactgg cgctgtcata atcccttgga tactatgacc
    29581 ctgctcagct actacgatga ctttagcgcc catcgcatgt aagaaatccc gatgtgcttt
    29641 gaaggcttcg gcagtttctt caaaaggttt agttgtcaga aacgcactga accatgcact
    29701 ggcaaccttt aagccgcgaa tattcaaata ttgttccaac acctctggat cttttggata
    29761 tttgttgcca atctctgttc cggtgaaccc agcaagggcc atttcactga tacattgttc
    29821 aaaggtattt tcactaccaa gctcgggcat atcatcattg gtccacgcga ttggtgcgat
    29881 ggctaattga atttgtttgg tcatattctc acactcctgc ttattttttt agttgtacta
    29941 aactcttcgt atcgaatgat tcttgacaag ccaacgcgat cttagttgat tgcaatccat
    30001 cttctgcatt aaccgctggt tgacgttttt cttgaataca tgagacaaat tcttgggctt
    30061 cacgaatgaa ggcttctctg aatcgttctg ggaagttttg tgaacatgga cggatgatcc
    30121 cttgatcgtt catgatcgtc acaagatttt tctcaggcgc ttgcgctacc cgcagcattc
    30181 ctttggttcc catgatctct gtttcgacat gatagccatg ggctgcgttt ctgccggcaa
    30241 ctagtaaagc tattgtttta tcgctcaggc tcatcattgc ggctccagtt tcaagctcac
    30301 cagcctgatc caattctgga tacgccgcat ttttcccaat cgcccacact tgatcgactt
    30361 cttggccggt aaaccagcga accaaatcaa tatcatggat cgccatatct aagaacaagc
    30421 cgccgctgtc gttattcatg gcaaatttga caaagctttc caagccttcg ctcggatcga
    30481 tcccataaca acgaatcagt gtgatttcgc cgatttcgcc atcttctacc attttcttcg
    30541 catactgata cgactcatca tagcggcgca tgaagcctaa catgaagacc tgttcaggat
    30601 gctcttcgat gactttttgg gtctttttaa tatcttcaac ttcaaggcct aatggttttt
    30661 cagtaaagac atgcttgcca ttttccatcg ccaagcggac ttggtcacag tgaaagcctg
    30721 atggtgaaac aataaagact gcatcgatca cggaactttt gaccatttct tcatagctgg
    30781 aaaaagtatg cttgacgcct aatttcttct gtgcgaatgc taattctgtt tcaactaaac
    30841 tgcacgcagc cgtaagctca caacaactga cacctgttgc taaattttgt gcatgtactt
    30901 ttcctaatct tcctaaaccg acgatgccta ctctaatgtt gttcatgaaa tacctccgtt
    30961 ttctgagaat tactcattgt atagtgctgg tcgttcatcc atttgaatca ttacttttgt
    31021 ttttgtttca cgagactcat gacaagccat catggtgatc gctgctacat acccatccca
    31081 agcggtagga ccgacaaccg catcattttt gatcgaatcg atccacagct tgaattcata
    31141 atcataggca tccaaaaagc gggtctgcca atctggtgaa acaggtgtgt aatttttttc
    31201 ttccgattta attcgtgtat aggctgggtc cgtcattcct acttctgccg tttcgaaaac
    31261 cacatcacat ttaatatcgt agccaaagcg gcaattcaca aagatttcca aatcaatcgt
    31321 tacaccggat tttgtttgaa agatcagcag ctgcggatca tgcaaatcgg catgggagtt
    31381 gctggttttt ttcggcaaga tcatttgaac agattcataa tcttcattta gcaaccagcg
    31441 aagcacatca atctcatgaa tcgcggtgtt gctgatcgcc atcggtgttg tgtagttttc
    31501 atctgcgcta ggatttctat gggcacaatg catcattaat gcttctccgt attgcttcga
    31561 ttcgattgct tctttcagtt caatgtagcc gcggtcatag cggcgcatga agccaacctg
    31621 aaccaatttc ttcccataag caacttccgc atcaacgatc cggcggcagc ctgctgcatc
    31681 catcgctaat ggcttttcgc agaaaacata tttcccatgc ttgattgctt ctaacacata
    31741 ctgctcatgg gtcgggtccc aagaagtcac aacaacgaca tccacatctt cagaagcgat
    31801 caatgcttct ccggtttcaa agaatttagc atcgacacgt ttggcaacct ctttagcagc
    31861 ttttacattg atatctgaga ctgctgtcac ataagcccct tgtgttttct gattgatcct
    31921 gtcgatatgc tcgatgccga ttgcacctgt tccgataacg cctactttta ctctcaatta
    31981 cttatccccc tgttctgttg tttaagcaca catagtatgc gcttacaaaa ttattcctgt
    32041 gtagctttta ttcttaaaag gtagagccct ctctttgctc cgccttttac tacccataag
    32101 cttgtacgta taattcctta atttgactat gactaggcac ctttttatta ttttgcggac
    32161 ttccgctggt caacgcatcc gcggccattt tgtctaaggc tgccataaac gcttgctgat
    32221 caataccata atctcgaatc gttgggatcg acagctgctc acagagctct tggatttttt
    32281 taatcagata atccgcagcc tcttccagcg ataaatcttc ggctgccgga taaataaatc
    32341 tggcgatcgt cgctaactca gtctgaatag catcctttga gaattcaaaa aagaccggca
    32401 ataacatcgc atttgatatc ccatgaggaa tgtgaaaaag tgcccccacc ggtcgactca
    32461 ttccatgaat caatgtgact gacgcattcg aaaaggcaat ccccgcctcc gttgaagcta
    32521 agctcatttg ttctctggct tcagtgtcgt cgctattttc atatgcctga gaaagcgtgt
    32581 tcaaaatttt cttcatcgct tccagtgaaa atagttgcgt tacttcattg gctttgactg
    32641 aaacataaga ttccaaagcg tgacatagcg catcgactcc cgttgccgcg gttacttttg
    32701 gtggtacagt tttcgtaaaa tcagagtcaa cgattgagat ctgtggcatg aaaacgagat
    32761 ccttcatcat cattttgact gctgtttttg tattggtaat aactgccaca tcggttactt
    32821 ccgaaccagt tccagcagtt gttgacactg caatcagcgg aatgcccgct tgcagctgat
    32881 caaaatttct tgcatgaatc gcttcgagac ttaattgatt agcagccaaa acggctacca
    32941 ccttggctgt atccagacag ctgccgccac caaaagcaat gatatgatca cattgatgct
    33001 tctgaaaaag cttcagcgca tcataaacat tttcttgcgt cggttcggct tgaatgtctg
    33061 tatactccgc taattgatca tctgtttttc ccaacagctc cttgatcttt tgaagatgcc
    33121 cggtctgttc cataaccgta tcactaatga ttaacggctt gttgcctagt tgttctgatt
    33181 cttccataag aaattggatt gttccgccgc caattcttaa attggcaggc attttcagtt
    33241 gaatattcac agaaaaaacc tctctctggc ttatagccta taacagaatc ctcgaagctc
    33301 tttaacagag cttcgaagca aatctttaaa tctcactttg cgcataacgt gctgtaacaa
    33361 ctttccgatg ggtataaaac tcaacactgt ctttgccatt ggcatgcaaa tcaccaaaga
    33421 aagagtgctt ccagcctgaa aaggcaaacc aagccatcgg tgcaggcacc ccaagattga
    33481 cccccagcat cccagcatca acattttctc tgaagtagcg aatcgctgat gcgttgttgg
    33541 tatacagaca cgaaccattc gcaaattcag attcattcgt cagctcaacg gctgccatca
    33601 aatcttttac ccgcacgatt gatgcgaccg gggcaaaaat ctcatcctgc cagatcttca
    33661 tatccatcgt gacattatca aaaatcgttg gtccaacaaa atagcctttt tctggaatgt
    33721 tttcgcgacc atctagtacc agtttcgctc cttcatcgac ccctttttgg atatagtcaa
    33781 aggtccgctg ctgtgcatct tttcgaatca ctggtcctaa aaagacccct tcgtccaagc
    33841 cattgccgat cttgatcgct tttgccgcag ccttaaattt ttcaacaaac tgatccgcga
    33901 tttcctcttc caaaacaatg acggaggctg ccatgcagcg ttcaccagca gaaccaaaag
    33961 ctccgccaat aaggtccttg actgcccgat caatattggc atctttcagc acaacaacat
    34021 gatttttcgc tccagtcaac gcttgtaccc gttttagatt ttttgttcct tcacggtaaa
    34081 cgtattcacc gacatttttt gatcctacaa agctgatgcc ttttaccagt ggatcttgca
    34141 gcaatgtgtt gacaacatcg tgggcaccat agacaacatt aaaaacaccg ctaggaaagc
    34201 ctgctttttc aaccagctca acaattttct ccatcaacaa tggtgttttt tctgaaggct
    34261 tcagcaccac cgtatttcct gccgccagag ccatcgggaa gatccagaag ggcaccatca
    34321 ttggaaaatt aaacggtgtg atccctccga ttactccgat tgggtaacga taattcgtga
    34381 tctcaatatc agtcgccgct gttgataaag aatctcccat cattaaattg gtgattcccg
    34441 ctgcgtgctc cacattttcg attcctcgtt gaacttctcc cagtgactcg gacaatgatt
    34501 tcccattttc cagcgtgatc aaacgcgcca attcactttt atgatctgtt aaaagctgat
    34561 gcagcttgaa cagataacgt ccgcgaacag gaacaggaac ttttttccaa gtttgaaacg
    34621 cagcatcggc agctttaact gcttcggctg tctctgcttt gctagacaac ggtacattcg
    34681 ccagcgcttc ttctgtagcc ggattcatta cttcttcaaa ctctgttgag cgactagcga
    34741 cccaacgacc atcaatataa tttcttaaaa atcttgtcat tta
//
LOCUS       DACIRY010000016        34605 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.016, whole genome shotgun sequence.
ACCESSION   DACIRY010000016 DACIRY010000000
VERSION     DACIRY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.016"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(311..2203)
                     /locus_tag="IYE22_000654"
     CDS             complement(311..2203)
                     /locus_tag="IYE22_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505887.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2200..3927)
                     /locus_tag="IYE22_000655"
     CDS             complement(2200..3927)
                     /locus_tag="IYE22_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505888.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3943..4593)
                     /locus_tag="IYE22_000656"
     CDS             complement(3943..4593)
                     /locus_tag="IYE22_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9505889.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4797..5093
                     /locus_tag="IYE22_000657"
     CDS             4797..5093
                     /locus_tag="IYE22_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ9505890.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5285..5905
                     /locus_tag="IYE22_000658"
     CDS             5285..5905
                     /locus_tag="IYE22_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ9505891.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5997..7163)
                     /locus_tag="IYE22_000659"
     CDS             complement(5997..7163)
                     /locus_tag="IYE22_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9505892.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7175..7528)
                     /locus_tag="IYE22_000660"
     CDS             complement(7175..7528)
                     /locus_tag="IYE22_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ9505893.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7656..8678
                     /locus_tag="IYE22_000661"
     CDS             7656..8678
                     /locus_tag="IYE22_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ9505894.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9117..9551)
                     /locus_tag="IYE22_000662"
     CDS             complement(9117..9551)
                     /locus_tag="IYE22_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505895.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9661..11520)
                     /locus_tag="IYE22_000663"
     CDS             complement(9661..11520)
                     /locus_tag="IYE22_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ9505896.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11638..12798)
                     /locus_tag="IYE22_000664"
     CDS             complement(11638..12798)
                     /locus_tag="IYE22_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ9505897.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13105..14529)
                     /gene="araA"
                     /locus_tag="IYE22_000665"
     CDS             complement(13105..14529)
                     /gene="araA"
                     /locus_tag="IYE22_000665"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ9505898.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14545..15243)
                     /locus_tag="IYE22_000666"
     CDS             complement(14545..15243)
                     /locus_tag="IYE22_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ9505899.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15200..16861)
                     /locus_tag="IYE22_000667"
     CDS             complement(15200..16861)
                     /locus_tag="IYE22_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ9505900.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17079..18479
                     /locus_tag="IYE22_000668"
     CDS             17079..18479
                     /locus_tag="IYE22_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ9505901.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18731..19810)
                     /locus_tag="IYE22_000669"
     CDS             complement(18731..19810)
                     /locus_tag="IYE22_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9505902.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19896..20855)
                     /locus_tag="IYE22_000670"
     CDS             complement(19896..20855)
                     /locus_tag="IYE22_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ9505903.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20867..22390)
                     /locus_tag="IYE22_000671"
     CDS             complement(20867..22390)
                     /locus_tag="IYE22_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ9505904.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22400..22963)
                     /locus_tag="IYE22_000672"
     CDS             complement(22400..22963)
                     /locus_tag="IYE22_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ9505905.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22969..23832)
                     /locus_tag="IYE22_000673"
     CDS             complement(22969..23832)
                     /locus_tag="IYE22_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9505906.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23846..24742)
                     /locus_tag="IYE22_000674"
     CDS             complement(23846..24742)
                     /locus_tag="IYE22_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9505907.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24917..26188)
                     /locus_tag="IYE22_000675"
     CDS             complement(24917..26188)
                     /locus_tag="IYE22_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9505908.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26178..27695)
                     /locus_tag="IYE22_000676"
     CDS             complement(26178..27695)
                     /locus_tag="IYE22_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ9505909.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27893..28783
                     /locus_tag="IYE22_000677"
     CDS             27893..28783
                     /locus_tag="IYE22_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ9505910.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28856..29680)
                     /gene="nadE"
                     /locus_tag="IYE22_000678"
     CDS             complement(28856..29680)
                     /gene="nadE"
                     /locus_tag="IYE22_000678"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ9505911.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29692..31164)
                     /locus_tag="IYE22_000679"
     CDS             complement(29692..31164)
                     /locus_tag="IYE22_000679"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ9505912.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31801..32163)
                     /locus_tag="IYE22_000680"
     CDS             complement(31801..32163)
                     /locus_tag="IYE22_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ9505913.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32386..33138
                     /locus_tag="IYE22_000681"
     CDS             32386..33138
                     /locus_tag="IYE22_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9505914.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33278..34201)
                     /locus_tag="IYE22_000682"
     CDS             complement(33278..34201)
                     /locus_tag="IYE22_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ9505915.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34216..34488)
                     /locus_tag="IYE22_000683"
     CDS             complement(34216..34488)
                     /locus_tag="IYE22_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505916.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10207 a         7209 c         5974 g        11215 t
ORIGIN      
        1 agaggttgtg agcctgccgt ttaactccga gtcacaagga gcaatttcaa aaaacagctt
       61 ctcaggtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa
      121 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agatacaaaa cggaaaaata
      181 gcttctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacaccgtt
      241 tattcggaaa aagggactgt gacacgactt ttgtcacagc ccctaaaaaa aataataaat
      301 ttgtactatt ctatgaagct tcttcttcag aaaactgact gttataaaga tctgcataga
      361 atccgtcttt ttccatcaat gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca
      421 tcacaataat gttgtctgca tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg
      481 tccggttttc caataggcgg ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta
      541 cactagatgt tgcttcatca aggatcagga catctggatt tgctaagaat gcccgcgcaa
      601 ttgtgattag ctgacgctgc ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt
      661 tatatccttc tggcaattta cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa
      721 tgatttgttc gtctgttgca tcgtcgttcc catatttgat attgtcatag atacttcctg
      781 taaacagcca agtatcttgc agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa
      841 gatcacgggt atctacacca tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt
      901 caagaaggtt gatcagtgtc gtcttccctg caccagtcgg acctacaata gcgaccatct
      961 caccaggttt gacatccaag ttaaaatcag tcattagcaa gttatctggt gtgtagccga
     1021 attgaacatt ttcaaaacga actttgtatg gtgtatcttt ttcttctgga aggccagatt
     1081 tttcattgga catctcttct tcatccaaca cttcaaatac ccgttcggct gaagcaactg
     1141 ttgattggat agtgttcatc aaacttgcaa gttgagtgat tggttgagag aactgtgttg
     1201 tatattgcaa gaacgcttga acatccccta atgtcatgtt cccatttgca acttttactc
     1261 cgcctagtac agcgacgaac acatagccta gatttttaac gaaattcatc aacggcatga
     1321 tgattgccga aataaattga gctttccaac cagcggcata cagattctga ttttcttttt
     1381 caaatacttc ctggtctttt tcagtatgat tgaatgtttt aacaatggta tgaccaccat
     1441 atgtttcttc tacttggtta ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca
     1501 cttgtgattt cggcgctacg atcatcacga tgatcaagct taaagggatc atcaataacg
     1561 cgactaacgt caattgccag ctgattgaaa gcatcatcac gatgacccca atcaatgtga
     1621 ctgcactagt tacaaattga gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat
     1681 cattgattgc tcgagacatg atatccccat ttgtatgtgt atcataatag gaaatcggta
     1741 cacgattcat tttggcttcc aaatcatgac gtaattcata aactgttcgt tgggaaacac
     1801 gtgtcataat aaattgctgt aaaaagttga atacagcaga aatcaaatac atcacgatta
     1861 ctgtcgcaat gatctgcgcg attttatcaa agtctatcgg aaattttgtt acttgttggc
     1921 cttgttgcat ctgttgagcg ccagccatta cacctttgaa tatttctgtg gttgctttcc
     1981 ccaagacctt tggtgtttgg atttggaaga tcacggctgc tattgctagt acaaatacag
     2041 cgatgatcgc aaccatacgt ttagacatat agccaaacaa tcggcgtacc gttccccaga
     2101 aatttttagg cttttcacct tttacaccga gatttctgcc aggtccacca tgtcctggac
     2161 cgcctgctgg tccatgagag ggacgtgtgt ttgttttact catgctaagt cctcctctct
     2221 tagttgtgaa tgaacgattt cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt
     2281 tcctttgcca accagttttc cttcatctag cacaaggatc atatctgcat ccattactgt
     2341 acttactcgt tgagcaacta aaacggtgat actttctttc atattcgttt tcaatgcttg
     2401 gcgaagattg gcatccgttt tgaaatccaa cgctgagaag gaatcatcaa acacatagat
     2461 atctgctgtt ttgactaatg cccgtgcaat tgccagacgc tgtctttgtc caccagagaa
     2521 gtttcctcct ccttgttcca catggctatc taagccatct tccatctctg aaacaaaatc
     2581 tttagcttgt gcgatttcta atgctttcca aatcatttca tctgttgcat ccggcttacc
     2641 atactgcata ttgtctcgga tcgttccaga gaacagaact gctttttgag gaacaaagcc
     2701 cattaattct cgcaaattat attgagtcat gtcactaatg tccgttccat ttatttggat
     2761 cgaaccagat tcgatgtcat ataatcgagg gatcaaattg actaaggtcg ttttacctga
     2821 accagtcccc cctatgattg cgacgatctc gcccgacttg gcttggaaat caatatcttc
     2881 caatgcaagt ttttctgctc catgatatcg ataattgaca tgattgaagg ccaatgttgc
     2941 gttttttcca gcaaaagaaa ctgttttagg attttcaggg tcatggatac cgattttttc
     3001 atccaacact tcattaatac ggtcagctga agcttgtgca cgaggcacca tgataaaaat
     3061 agcggaaagc atcataaagc tgatcaagat ctgcatcgca taagtcataa atgcaatcag
     3121 attaccgact tccaaagtca tatctgctat gtagtgtcca ccaaaccaag tgattgctac
     3181 gtttgtaccg ctcatgatca atgtcatcat tggcatcatc aatgcgacga tcgtattaac
     3241 tttgattgcc gtatttgtat aatctttatt cgctaaatca aaacgatttt cttcaaagcg
     3301 tgtcttatcg aatgctcgaa tcactctgac ccctgtcaat ccttctcgaa ataccaggtt
     3361 taaacggtca gttttctttt gcatactttt gaataatgga acagcaaagt acatgattcc
     3421 gccgatcaaa ataatcatta ctgggatgac aactaagaag atttttgtca actgatgatc
     3481 tttttgataa gcaagtatgc tcgcgccaat caacgtgatt ggtgcattga tcatcatacg
     3541 taaaaacatt tgtgtaacca tttggatttg agtcacatca ttggtcgttc ttgtgatcaa
     3601 agaggccgtt ccatatttat caaacgcatc ttttgtcaga ctctctgatt ttttatagac
     3661 atccgtacgc aattgcttac ccagtttctg tgattcacga gtagcaaaaa atgtatttgc
     3721 gatagcagct aagatactga tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat
     3781 ataatcgaca tctccttgcg ctaccccttt atcaataata ttggaggtaa gtgtcggcaa
     3841 atataaatct gccagtactt ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa
     3901 ggagatccgt tttacgattt ttagcatcta gttcccttct ttctatttat atactccgtt
     3961 tttcagccaa gaaagtttca aattgaattc actgattgct tgctctactg agtaatcttc
     4021 agatgttgaa aataagcgat acgaatgcgc aattgttgta aatacagtat tcatcactaa
     4081 ttggatcagt aattccaatt catgatcatt ttttactttc agcttcgtcc gatcgatcaa
     4141 ttcaagcaac tcttttgcat cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg
     4201 gcgactcgta tgaggagcga cttttcttga ggcacggtaa tccatgttca taaacaagtt
     4261 acgataaaaa gaagcattct cgccttccaa aatctcataa atcattttag aaaaatagga
     4321 ttcgaatcct ttgaatagat caccatcgga ttctttcatt agttgtaaca tatctttaga
     4381 gctgtcacgt ctcattgtct caaagtaata aaaataaaga tcctctttat cttcaaaata
     4441 ctgatagaaa ctgcctctag ggatatccgc taatttgacg atattagcaa tcgaagcttc
     4501 ttttaagggt actctagaaa actcgattct cgcagcctcg attagtcttt tttgtttttc
     4561 tttaggcaaa tggaaaaatg tctgtgtcgg catatggatt ctcctttctt tcaagatgac
     4621 acattgtcat atgacgctat gtcattataa cacctcctac ctgttttgca agacatttgc
     4681 atagtttttt tcattatcat ccgatacatt aaaaaagtga taagaggaat ccttccattt
     4741 gaattaatga gagttgccca ttaaaatagc cacatactaa acaaaggatg agataaatgg
     4801 cccaatcaat tagtgtccaa gacttcaata aattgcctct cgatgctgac agcatcgttt
     4861 tagatacaag aaatacctta gattacacaa aaaaccactt aagccactcg ctttctatcc
     4921 ccgctgctat cttgccaaga caattaaaaa atctagatcc aaagaaactt tattatattc
     4981 taagctatac aggcaggcga tcagaagcat tagccgatca acttactgct aatggttttc
     5041 gtgctgttta tatcattggt gggatggaag ctcttcagtc gagtgttgct tgaatattgc
     5101 ttgttagatt caaaagaggc tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt
     5161 ctttttttag ttatttattc ttcaacctta tcaatatgac ttgatagcct tttctttttc
     5221 gagtaaactg attttgttag tcatagttat aacaatcata gcaataaaag gagaaggtaa
     5281 gcatatggca tctgttttag tagtcaaagg tcatccccta actgcagaag aatcgcgcac
     5341 agttaaggca ttgacgtctt ttttaacaag ttataaagaa aatcatccag atgatgaagt
     5401 gactgttttg gaactttatc gcgatgatat tccggaaatc gatgaagaat tattatcagg
     5461 ttgggaagca ttacgtgctg gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa
     5521 aattgctcgt ttcaatgaat tgactgatca gttccttgct gctgataaaa tcgttatcgc
     5581 aaacgcctta tggaatctaa atatcccaac aaaattaaaa gcttggttcg ataccgtcaa
     5641 tgtagctgga aaaacgttcc gctatacaga aaacgggcca gaaggattag tgacaggtaa
     5701 aaaagcactg cacatccaat ctaatggcgg tgtatacaac ggacaagact ttgcctctca
     5761 atatgtaaaa ggtattttaa actttgtagg catcgatcaa gtagatcagc tattcatcga
     5821 aggaatcgat tatgaccctg accgtgcaga tgagttgatg caaaatgcat tagataaagc
     5881 tgctgcttta ggtaaatcat tttaaaaatt gagataaatg catacaaaag agacagagga
     5941 aaaaatcttc aaagcagcat ttgtgccttg gatattttcc catctgtctc tttttgttat
     6001 tctttctgct ttttcaacgg attttcttca taaagagaag agattgttgt gtaccattca
     6061 aaaacatgag taatcactac tttagctgct gcataaacag gaatccccaa gatcacaccg
     6121 atgattccga acaatttccc agaagtcagc agaactaata aaatcgttac tggatggatt
     6181 gctaactgat tccctagtac taatggagag atcaagcgtc cttctattgt ctgctctact
     6241 gcaaaaacga tcagtacttt gatgatcatg aatggacctg ctacaatggc taaaaagata
     6301 gccggaatca tcgctaaaaa tgatcctaag taagggatca ggtttaaaaa acccgcaata
     6361 atccccaacg tgacagcata ctctaaacca atgaccgcaa aaccaatcat aaacatgatt
     6421 gccacagcaa aagccacagt tagttgtcct cttatataag aagaaacttg actattcatt
     6481 tcactcagca cattcaacgt cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa
     6541 ggtgctaatt gtttaccgtc tttcaataga taaaataaga taaacggcat cgtcagcaga
     6601 gccactaaaa tagtcgctac cgcgccgaca aaacttccaa gactctgaac tgtactctta
     6661 gatatatctt ggatcatatc gcctgccgaa ttcatgaact tatcacctgc tgtttccaac
     6721 tgttcgcgaa attggttgaa cagcgggtcc cgcaaaatct cggttaattt attatcaatc
     6781 gtatctacat attgagggaa gttactgatg aagctcactg tttgttcctg aatcttaggg
     6841 atgatcacta cgctccccca tgcgattagt gcaacaacca aaacaaacag cccaatgata
     6901 ctgtaaagcc ttggtacttt tcgcttttca aagtaatcaa ctaccggatt catcaagtaa
     6961 tacaagattc ctgctaaaat gataggcaga cctacgattg ctaaaaattg ccagattggt
     7021 gcaaacaaat atgaaacttt tgtaaataaa aagacgatca gcaatattaa caatacaaca
     7081 agtaaggctg tgactacttg attatttaaa aaccagcgcc aaaaccacga taccggtctg
     7141 ttcttcttat tttttccttc cataattcct cctgttagtt ataaaaaatt tggcttccca
     7201 aaccaatagc aggcagtcta tgcggatgaa gatagattcc atttgcaatt tgtccttctt
     7261 ctggacaagc atcaaaaatc aatgatacgt gagctactgt ctttaaattc ccattggcca
     7321 tcggaccgac ataatcgatc gtatatacct gatcatcaaa agcaattgtt ccgcctgctt
     7381 tcaaagaaaa aggttcgtct tttgatactt cttgaatcac cgaatatttt cgtaaggcag
     7441 ctgttgctgt tttatcgaac aaaatcagca tgggttcttc cttgtttatt gcttctgtcc
     7501 caatttctgt aatctttgcc tgaatcattg cactctctcc tttaattgct ttcaaaaaca
     7561 gtataacaca ttctcattct ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa
     7621 aactatttca ttacaattag gaggaatcag ctcatatgat cgaaaagata attttaggaa
     7681 cctatacgcg tcgcgaaagt aaaggaatct atacgatcga ccttgataca gaaaaagaag
     7741 aattagccaa cctttcatta gctgttgaag aaaatagccc tacctatgta gcaaaaagta
     7801 aagctggcaa tctttacaca gttactagtg tggatggatt aggtggtgcc ggtgcgtacg
     7861 ataaggattt tcattttttg aatgcggtaa cagagtctgg cgctcctttg tgttatgtag
     7921 cggttgatga agcacgtcaa ttactatacg gggcgaacta tcataaaggt gaagtaaatg
     7981 tctaccatat attaggagat ggcgaactga aagcagcaga ttcaatattc catcaagaag
     8041 cgacaggtcc tcataaaaac caagatcatg cgcatgtcca ctatacggat ctgactcctg
     8101 accaacgatt agtcgtatgt gatcttggta cagatcgtgt gtatacttat gatgtgtccg
     8161 aaaacggcaa attgaatctt gtaactacct tcactgcaga gccaggaaca ggccctcgcc
     8221 atcttgtctt tcatccaaat ggacaattcg cttatttatt tggtgaatta gacagcacag
     8281 tccgcgtgct ttcttataat aaagaaacag gtgcttttga agaattacag aaaatatcga
     8341 ccttgccaga agaattcgac ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg
     8401 gcaagttcct ttatgcttct aaccgaggac ataactcgat tgctgtattc gcagtttttg
     8461 aagatggttc ccacattcaa aacaagcaaa tcatctcaac agaaggggat tttccccgcg
     8521 atttcgcact agatccaagc aataattttg tcgtgtgtgc caatcagaac accgataatc
     8581 tgacattata tcgccgtcat cctgaaacag gattactgga attactgaaa aaagatgtct
     8641 atgtaccaga atgtgtctgt gtttattttg atcgttagta agaaaaggag aatgaaatga
     8701 atcagcaaga cgtattagac agactacggg aagaactaaa tattcctttc tttgatggga
     8761 aaatcgaaga taaagagtat agcgaagagg aatatcagaa actcaaagcc gatttacaga
     8821 actactttga acaatatgta cagaatgtag aaaactaatc agctttccat actatctgaa
     8881 aagaacatca ggacaagact tttccgtcta tcctgatgtt cttttctaca aataaaaaac
     8941 cgccgagatg gttagactcg acggaaaagg agtatttact cgattttgag taagtacaag
     9001 gttgttgttg gttataggta tgtcattatc ataaaggatt ttgatcactc tatcataaag
     9061 gtaatattac atttatatta gataactcta gtttttattg ttatcaaaga tttcttttat
     9121 tttttcaaca acaaattagt cattttttgg atggcagctg tcaattggtc tgtcgcttgg
     9181 tcttgttttg cttcagatgt attgaataga tcttcatcta cccctgattc aagatcgata
     9241 ctttggatca tttcttcaca gcctgctaca tatgaaagat aagctttttc aaaattttta
     9301 tgaatgccca ttgcttttgc tggtggacgc aaagtactga ctttcataag cattgcccga
     9361 tatttttttg ttccttcttc aaataacagg atgatctctt caagagtcga tttgtccagt
     9421 tcacttgttt ttccttcatc aattgcgttt ctgatcactt catatttcgg atgcattcct
     9481 tctccgattg cttctgtttc cttgacgatt tgatcgatgg actgcacata atagccaata
     9541 ttcggtaaca tgttcgtttt cctactttct ccattgttgt tattatttat tctacttgtt
     9601 tttatcctca aatgacaagt acttgaaaaa atttaatggt ttctatacag ctcgccattc
     9661 ttattcgtgc tcaaagaaac catctgctaa actatattgt ttccctgcga caatgtcata
     9721 ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt
     9781 ataccactgt ccgttttgat agtacagttc tcgttcaaac gccggaagtt cctgttctaa
     9841 tgataaaagc agctgataaa agccatttgt tgtctgattc gtctgttcca ttagattcgc
     9901 tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt tgcttttcta attctccttt
     9961 actgtcgaac ataagaaatt gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat
    10021 cgtatctgaa tagatccctg gaagatgatc cccccaaaaa acaactaaag tccttctaga
    10081 aagattcttc aattcttccg tgaactgctt cagtgccgtg ctgctatagg aaatatcttg
    10141 cagataattc tctaagtcaa gtgtcccact tccatctgaa gtttttgcag gatagtctag
    10201 attatcatac tttccatcgt agggcatatg tgtctgcatc gtgactaaat gaatgaactg
    10261 aggtgttttt tcttccttga gaagtgtcat tacttctttg taagctgact catcagaaat
    10321 atatggatta ttctctatcg tatccgtata ggtcatcgta cgctcgctga tgaactggtc
    10381 aaatcctaat gtctgataaa catcctctct tttatacatc gaagtattgt atggatggat
    10441 cgctgtcgtc tggtatgatt gtgcgtttaa cgctgaaacg atagaaggaa gctgatccat
    10501 ttttggtaca agcatcgtgt aaggggtggt gagttgcgca ttaaataacg ccatagaaaa
    10561 accagtcaat gcttcaaatt cgatatttgc tgttccaccg ccatagtttt gagaaagcat
    10621 attgccacta tatgtttgat cagctacttc ataataatct gccaatggct caccagatac
    10681 ttctacgccg tttaatcgac taggatctga gaaactttca ctcatcacga acacgatgtt
    10741 cggcgactct tcctctacag ttttattttt ttcatcggct agcttctggt atttttcagt
    10801 gatttctttg atcttttctt tcgaatatcc ttcaggttca tccattggtt ctaccttcag
    10861 attgtacaaa aacccaccga taaaaccagt gttatagtag ttcattttct ggctgtaagg
    10921 tatccatagt gccgttttat tatatgcttt tcgcaacaaa ttatcgggat tgttgaaatt
    10981 actgatatac cctagcagtc caattgccac aagcagtgtc gttaatcgga tcaattggaa
    11041 tcgccttcct tttttcaggc ttcgaaacat gtaccagcaa aacaatccta tcacggttcc
    11101 tgcggcagct aagatcactg taaacagcat agtgccggtc atatctttca gcaatccgat
    11161 ttcagtgatc atcttcaaat cgtctggata aatcggttct tgccgataac tcattttcat
    11221 ataatttgca aaacctaata caccgatggc tacgacatac gtaagggaac ctaaagggac
    11281 agatcctgct agtgcaatca gaaaaataag aataatcagc aagacgagac aggctaagaa
    11341 gaatttttcc gtatgccaag aaaaagcaaa tttcaacgca aggtctacgg atagatcatt
    11401 ttgacaccac tgcaaaaata aattactaaa aacaacaaga aaaaagatac tgatgattgc
    11461 tgctgttcct tttaaaatct ttttctgttt ggtcgaaagt ctgcttatat atcttttcaa
    11521 atgcttgctc cttcatttct atcaataaaa gaatgttgct tctcttattt tagttgtacg
    11581 aaattcgaaa gtaaagaacg gaaagaagaa ttaatgaaat ccaaatattc tctttcttta
    11641 tggcctttct ggtttcttta ataagcggac ctttcgtact ccagaaattt tttctagctg
    11701 ttgtttcact tgattgagct gttcaaaatc agtactgtct atgtcaatca atatgtagac
    11761 atgctcattc ttatggttac gcgatatatc tgcgatcgct agctcgttct cattcagcag
    11821 agaaaatatc tctgctaata tattggtttt cttttgataa aatatagtga atcgaaacgg
    11881 cgcttggaaa aacattcgtg cggatggata attgaccgct tctctaaccg taccgaacaa
    11941 aagataatct tttaaggctt caactgccaa acgtccgctg tctgccaaag cttcctgagt
    12001 cgatccccct agatgaggca acataagtat acggtcattt tctaagaact cttcttctgg
    12061 aaaatcagtc aagtatttag ccagatgttg tttttccaat gcttgcataa cagcatattt
    12121 atcaacaatt tcacttcttc ctacattgat aagtacagca tcttttttca ttttttctat
    12181 attcgcctga tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga ctacgatgta
    12241 atccgaggta gaaagaactg tttctaaatc tgcttgccga acataatcca actgggcatc
    12301 gcggatagaa taacccaaga catccatccc taagctatag caacttagcg ctacttttgt
    12361 gcctatcgct ccaagaccaa gaagaccgat ggttttgcct tgcaattctc gtcccacata
    12421 agcacttctt ttgttttctg cctgttctaa aatattagga cctgttagtg tttgtaccat
    12481 acgacttgct tcgattatcg gacgggaaga taagagcaaa cagcacaaaa caagctcttt
    12541 tactgcatta gcatttacac ctggtgtatt catgacgatc gtcccgtttt cagaagcttt
    12601 ttcaacgttg atcgtgttca ccccaacacc agctcttgaa attgctagca aatctggtgt
    12661 aagccattca tctttcactt tcgtactacg aataactatt ccttcggctg gcaaatcatc
    12721 tattattttg aaatattccc cttgtaactt ttctatctct tgttctggaa tagcatcaat
    12781 caaatgaacg tgatacataa aaccccctcc ttactaacaa gtatctcgaa atttctgcaa
    12841 ttagacaact gtttaccatt tgacttccta gaagcaaaaa gagcagttag agcagagtag
    12901 gctgagtgat aaaaataagg ctatgacaag ttactgccat aagccttatt ttcgagagca
    12961 aaatattgag ggtgagatat cttgataaat ggtgttccgt tagctgacat tgataataag
    13021 actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact
    13081 aagtggcttt ttcacaacct ctttttatct aataaccact gtttccaact caactaattt
    13141 tgcccacgtt tcaatttgat ctgttgttag atttagcgag acaacggtat gatgaccgcc
    13201 acctgcctga atccatgaat ggacaccttc atggaaattc ggtttagttt tccataacac
    13261 acgtgcaaca ggtaaattag gtgcttcagt cgttggttca aaggcttcta cttcattgat
    13321 cagcagttta taatgcgtac cgaaatctgc catcgatacg actacccctt ctcctgcttt
    13381 cccatcaaat acaagacgcg ccggatcttc tctatctccc attgataaag gagaaacaac
    13441 gattcttggt ttcgtcgcag caagcgtcgg atctacttcc atcatatggg attccaagat
    13501 tgcttcttgg ccactagcta gctcgtaagt ataatcttcc atgaatcctg tatctttccc
    13561 tcgagccatg attttcagca accgatcaat cgctgcagtt ttccagtcgc cttccccagc
    13621 aaaaccgtat ccttctgcca tcaaacgttg tacagctagt ccaggtagtt gtttcattcc
    13681 atacaggtct tcaaaattag tcgtaaaagc gttatagcct ccttcttcta agaaacggcg
    13741 aataccgatt tcctgctgag cttgtacttt tacatgttcc tcccatgtct tttcttcata
    13801 atcaccatag tcgaaggtat acaattcctt gtactcttca aataattgat cgacttcttc
    13861 gtctgatacg cgatcgatta cttgtacaag atctccgata ccaaaataat caacagtcca
    13921 accaaattga atctgtgctt ccactttgtc tccttctgtg acacccacgt ttctcatgtt
    13981 gtcaccaaaa cgagccactt ttattccaaa actttcatta taggctactg caacatccat
    14041 ccaatcagca atctcttttt ggatctcagg tcgtttccag tagccaacga cgattttatt
    14101 ttgtttattt aatcgagcat tgatgaaccc atattcgcgg tcaccatgag cggattgatt
    14161 taaattcatg aaatccatat cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc
    14221 taaatgaagc aatggttttt gtagaagctt tgtcccgcgg atccacattt ttgctggtga
    14281 gaaagtatgc atccaagtga tcacacctgc tacttcttct cgataattga cctctttcat
    14341 gatttttgtg atcgtatcag cagtaactgc taattcttga aggcggattg gataaggcaa
    14401 ttggccattt tcattcagtt catcgaccat ttcttgtgca tgtttcttta cttctcgcag
    14461 tgcttcttcc ccatataaat gctgtgatcc tacaacaaac caaaattctt tttctccaat
    14521 attcaacata attagccact ccttttattt gttttgtccg taatacgcat tttttccgtg
    14581 ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag
    14641 ctgtctggta aataaattca tcttgcatac ttcatctagt acgactgcgt tcattaccgc
    14701 actttgcgca tctttccccc aagtaaatgg accgtgtccg tgtaaaagta cccctggaat
    14761 tgctaaggga tcaattttgc gttctttgaa tgtttctatg ataacttttc ctgtttcata
    14821 ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta ccgatccata
    14881 aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt gtgcccagat
    14941 cgtcgcccat gtcgagtgag tatggacgat tcctccgatt tgaggaaatg aacgatataa
    15001 aacagcatga gtaggcgtat ccgaagaggg attgagttta ccttcaatga tatgattgtc
    15061 caagtccaca acgaccatgt cgtctgctgt catctcttca taactgactc cgctaggttt
    15121 gataacgaaa tatcgtgttt caggatcaaa agcgctcaca tttccccatg tatatttgac
    15181 gagtccgtgt ttaggcaagt caagatttgc ttggtatact tcttctttca gttgttccag
    15241 cattttctat cactcctttt tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc
    15301 cctcacggta acgtctcata aatatctgat agcctgctat ttcttctgct gaaggtgtga
    15361 tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa gaagttttct aaagacttcc
    15421 catgtcgatc catcatatag gctgctaata acgcgattcc ccacgctcca ccttctccgg
    15481 ctgtgtccat cactgtaact ggtgcctcca tagcagatgc tagaatccgc tgtccaactt
    15541 ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc aatttggaca ttctcagatt
    15601 tcaatatatc cattccgatt ctcattgcac caaaagcact ggctaaatgg atccgcatga
    15661 aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa taatggcctt ccttcattta
    15721 ctcctgtaat gttttcaccg gaaaaataac cataagaaag taatcccccg caatccgtat
    15781 cgccttctag cgctttgttg aataataccg tgaataattt atctctgctt atctccatac
    15841 ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc attgatatcc gaagagcagt
    15901 tattcgtatg aaccatgcct actagttctc ctgaaggtgt agtgaccatg tcgatttccg
    15961 gatggacatt ttctagttct ttttctaata cgatcatagc gaaggcggaa gtaccagccg
    16021 aaatgttccc tgtccctttg gctacactgt tcgtagcgac cataccggtt cctgcatccc
    16081 cttctggcgg acaaaccggg attcctggtc tcaaatttcc cgtagggtcg atcagataag
    16141 cccctgattc gcttaacttg ccagcttctt ctcctgctac tagaacttta ggcaaaatgt
    16201 gatccaagtc aatcgctacg tgcgcttttt cgattaactg accaaaaata tttttcattt
    16261 cttgatggta ggttttcgtt gaaggatcga taggaaacat gcctgatgca tctcctacgc
    16321 ctagtacttt ctcaccagta agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat
    16381 taatttcttt cacatgttct tctttattca aaatagattg atacaaatga gcgatactcc
    16441 atctctgcgg aatattgtat tgaaattttt ccgttaatat tcgttctgct tcttcagtga
    16501 ttgtatttcg ccaagtacga aatggtacta gtaactgttc cttgctatca aatgctagat
    16561 aaccgtgcat cattgcactg aacccaatcg acccgattgt cgtaagattc tcgccataga
    16621 tttcgaatac ttctgctgcc atttttcggt aactgacgcg cagtcctttc caaatatctt
    16681 ctaatgaata ggtccagata ccattttcta gtttgttttc ccactcataa ctaccagcgg
    16741 caataggagt aaaagatgaa tcaatcaata cagctttgat cctagttgat ccaaactcaa
    16801 tccctaaaga tgtacgacca tctatgacat ctaacatact ttgcgcattt acttccttca
    16861 taatgattta actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt
    16921 acaatgatta ttgtatactt ttgtacgtac atgtcaagca gttatttaca agaatacaat
    16981 aaaaaacact tgttttgtac gtacataagt tataaaatga aattgcttac aaaatctgat
    17041 caaccgcctt ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt
    17101 cctcatcgtt tatttatttt tttggcgcat ttggcggcat tctatttgga tatgatattg
    17161 gtgttatgac cggcgcctta ccttttttac agcatgattg gggtttggca ggtaaagctt
    17221 ctttgatagg atggatcacg tcttcggtca tgttaggtgc gatccttggc ggctctttat
    17281 ctggtcttct ttctgataag cttggacgac gaaaaatgat tcttctttct gcacttattt
    17341 ttatggctgg ttctgtcctt tctgcatctg cccctcacaa tggttcttat tttctgattg
    17401 cagcaaggat tcttctaggt ttagctgttg gcgctgcatc tgccctagtt cctgcttata
    17461 tgtctgaaat ggctcctgct agactaaggg gaagattgtc agggatcaat caagtgatga
    17521 ttgcttctgg catgctcctt tcctatgttg cagattattt gctaaaaggg ttgcctgaaa
    17581 cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg
    17641 gtgttttagc tctaccagag tcaccgcgtt ttcttatgca atctggccgc ttggaagagg
    17701 caaaaagagt attgaactat attcggacac caaaagaagc tgaacaggaa ttcgaacaaa
    17761 tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact ttgtttttag
    17821 aaaaatatcg ttccttagtc attgccggta ttggtgtagc agtattccag caatttcaag
    17881 gagctaatgc tattttttac tatattccgc tgatcgtaga aaaagcgact gggcatgcag
    17941 cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat tttagttgct ggttctttgc
    18001 tctttttggt tattgcagac aagttcaatc ggcgaacttt attgaaaata ggcggtagcg
    18061 tgatgggact ttctttcatc ctaccagctg ttttaggaac tgtactggat gctcatacaa
    18121 attcgttgtt gattctcttg tttttatgta tttatgtcgc attttattct tgcacatggg
    18181 ctccactgac atgggtaatc gtaggcgaaa tattcccact agctgtccgc gggcgagcat
    18241 ctggcctagc ctcttctttt aattggattg gttctttcct agttggcttg ctttttcctg
    18301 tgatgactgc ttctatgtct caagaaatcg ttttcggcat ttttgggatc atctgctttt
    18361 taggggtttt atttattcaa gagatcgtac cagaaactcg tgggaaatct ttagaggaaa
    18421 tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat ttctatccat cattcttaaa
    18481 gaggttgtga cagaagtggt cagcttcaag aaataagaag gaaccgctta gtttccagtc
    18541 acaaggaaca atttcaaaaa acagcttctc atgtttgatg aatattgtga cttgtgacca
    18601 gttggtagga gaacaccgtt tagtggagag gtggtgaaag aaacgttcag ccttgagcga
    18661 tgacaaaagc tctcacaaac attgcgacga gtaaccgtag gagcaataag aggcggtaaa
    18721 aacccgtctc ttatttttcg ttttctttca ctgtttcccg ttcaactagt acaggttcat
    18781 agagaatatt tggctgtgcc tgtccttttt caatcgttgt gacgatccac cgtgcggcat
    18841 cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa ttttttattc cctaaatggc
    18901 ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc tggaatatga tagccatttt
    18961 ctgtcaactt atctagcatt aaactagcta tttgatcgtt atagcaaatg atgccatcta
    19021 aagcttgttc ttctttcaac cgttgtactg ttttttcagc gattgtttct cgtgtttccg
    19081 tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt atactgttca cacgcgctga
    19141 taaaaccttt catcctgtat tttccttgaa gatcgtcgat ttttgtgatg aacaacagac
    19201 gctgatgtcc tttttttatc aaatattctg ctgccataaa accgactttc acatcatcta
    19261 aacaaatata agaggcattt aactcttcat aagcagcatt gatcatcacc atcggaatgc
    19321 cttgttctct caaagcaaca taaagtgcta agtttgggtt atactgatta cttttcgtag
    19381 gctcaacgat caaaccatct accccataag caatcatttt cttcaaacac tctttctcca
    19441 attcgtgatc gttattcgta ctggctaaaa gcaatgaata cccagattct cgtaattcat
    19501 tttcgattcc tcggataatc gacggaaaaa tataatcaga gagatatgtg gtgatgacac
    19561 cgatcgtttt tgttctgttc aatggtgttt tttctttctt atagctgtca tctacatatg
    19621 tgcccgaccc tttttcttta cgcagatagc cctcatttac caacacagcg attgcttgcc
    19681 gaatcgtatg tctgctgacg tcatatattt cttgcagctt gaattctgag ggaatggttg
    19741 tatttacttc gtattcctca cgaaggatct tttgttttat ctcttctgca atatcttgat
    19801 attttgccat ttgccttgcc cctttttatt tgtacgtaca tatatcataa cgagtctgca
    19861 aggtttattc aagcaaaaat caaggtttct tgtgctcata aggcatacgg aacaggttca
    19921 ccaaattctg gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta
    19981 ggattgtcta acggatcccc tttttggttc ttctctggtc gagcatgata gatcaaaata
    20041 tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac caggaccaaa ttgcttattt
    20101 ttttcagaag aaacgaatac tggctccgat aatttatgcc atgattcttt taataaaaga
    20161 ttcttgtgct catcagccca cagcaggccc atggcatagt tttcatctgt tgcactacca
    20221 gaatacgtaa tgaatatttt cccattgcga gtaaggactg ctggaccttc atttactgaa
    20281 aaaccccgtt tttcccattc atactcaggt atagaaagga ggatctgctt cccctttaac
    20341 gtccaaggat tatccatttc ggatagataa agattagaat tcccagtaat ttcaggatct
    20401 tgttgggccc acacataata tagctttcct tgagaagaga aagttgttcc gtctaaactg
    20461 aaagtttccc ttttcgtttt tatttgtccc ttttctatcc aatctgtatt cattggatct
    20521 atatgctgat tttcaagtac gaacatgcgg tgatgatgag attcatcacg tatagtttta
    20581 tgattgcttg cagcaaagta aatataccat tttccattga tataatggac ttcaggtgcc
    20641 cagatcaatt cactcatcgg tccttcttca tgagcctgcc atacaatcag gcttgcagca
    20701 tgttctaatt cattcagtga ctttgccctt cttaattcaa tcacttggta gcctggaacc
    20761 gatcctgtaa aataataata accatctgta tgtttatata cccaaggatc tgcccgttgc
    20821 cgaacgatag gattattata aagtgtcttc atcataacaa atttcctcac attttcttta
    20881 ttctaattac attccaagag tatttttcta tccctacatt taattcgtca tgtttttcta
    20941 ttgaaaaata gtttatttca ggttttattt tttctggttc ttcacgtgta tttttgtcca
    21001 acagatcgct actggttaat gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt
    21061 caatcgttat cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc agctgattgc
    21121 cgttatctac gatcacactg tccacataag gaacattttt tatcttttca caatcataaa
    21181 cagggcaaat gatttgttgt ttcaatactt gacctttagc atatttcgat aagtgttgca
    21241 tcacataata agttggattc ctccatgctt gtcctttagc atctgtcagt attaaaggta
    21301 ttgtgtttac taacaatgat tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag
    21361 ccagccctaa tgaaccaaat aataatgtat cttccatagt aaagtaacac caatcgatgg
    21421 gactaccgac ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca ttccattcat
    21481 caaacgctag atacattgtt ttctcacttc tcaaatatcc ttttacataa tcacaagtag
    21541 caacgatttc ttcaatttga cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat
    21601 catcacgctc atttttagga ggtttagtca agtcttcttc atcatactta tcaatatagt
    21661 tatgcaaagc taaataatct acattctcat aagcttcttc taatgcgata cgatcccatt
    21721 cagggtacga tggtaaacgt ggcgtagagc ttccaactaa gattgtttca attgaatcat
    21781 ccactaactt catgactttt gaggcttcat ttgctaacct accatattca taagcactct
    21841 tagcccccac ttgccatgga ccgtctagct cattccctaa acaccataat ttaacgccaa
    21901 aaggcttttt ttgcccattt tttattctta aatcactcca gaaggttcct tttggaaaat
    21961 tacaatattc tactaaattt tttgcttcat caattccttt tgttcctaaa ttcaccgtga
    22021 atatcggttt cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat
    22081 ttggttctat acctcgccat gctaaatcaa ttttttttgg tcgttgttct ttcctaccta
    22141 cagtatccac ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta
    22201 attctaattc ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt
    22261 catggcccgg ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc
    22321 caaacagccg agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt
    22381 cttcgtccaa cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt
    22441 tccatacgcc accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa
    22501 atcctaataa caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccatg
    22561 cactttgttc aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc acatatatcc
    22621 aggataaaag aaacatggaa taaataagta aaactacata tccactaatt gaaagtacga
    22681 ttgtctgttc ttctaataat tgattaacaa gaaaataaat aatgatcgtt aacagactaa
    22741 ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa atgatatttc
    22801 ctgatccctc acctttccat tgcgccatta cataaaaaac agcacatgta gcaggcacga
    22861 tagtaataac aggcaaacac acgattaccc agagaatacc taataagatg taatcagaaa
    22921 ccacaatcaa ctgtccataa agtttagtct caactatttt catcttcctc actctttcat
    22981 tccagacata gctgttcctt gaacaaaata tttctgtgca aaaatataaa ccactagtaa
    23041 tggcagcatt gaaataaccg ttccagccat tacaggtcca taaaaagaaa tgttctgccc
    23101 tgtaaataaa gctaaccctg ctggtaaagt tctcattttt gaactacttg tcaatatcaa
    23161 tggataaagc aagtcattcc aactattcat cagcgtgaaa atccctaatg ataaaagagt
    23221 aggtttgctc aatggcaaca tgattttcaa aaaaatatta aactcactta atccatcaat
    23281 gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa aaggaacgca tcatgaagat
    23341 accaaatgca gttgtcatac gaggaatgat caaacctgca taagtatcaa gcatgccaag
    23401 caaattgact tcaataaata gaggaatcat gaagacttgg aatgggatca tcattgtcag
    23461 caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa atgcataccc
    23521 cgcaagagaa tcaaagaata cagaagtgat cactgtccct ccagcaaaaa tcactgtatt
    23581 tttgatgtaa tctaataaag ggatcgttgt ccaaacctta atatagttat ctagggtata
    23641 gctatctgca aatattctag gcggaaatga gataatgtct ttttcgtgtt taaaagaaga
    23701 aataatcagc cacaaaaaag gaaacgcaat aaccaacaat ataactagca tactaatcgt
    23761 tcgaatgatt tttttaccac taactgcttt tcgactattc acagtaactg taatcgagct
    23821 ttctggtgcc atacaggttt ccctcctatt tcatttgttt ttctttccgt gacatgtaca
    23881 cattagtgga gatagctata gttgcaacaa tgataaacaa aaccgaagac agtgctgatg
    23941 catagcccaa ttcataaggt gctgtaaatc ctcgactgta aatatattga acagctgttt
    24001 ctgtcttaaa ttgtgggcca ccagcggttg caacatatac ttggtcaaac acttgcatcg
    24061 aaccaatcag attcgttaaa atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa
    24121 tgttaaaaaa ctgctgtcga tttgttgcac catccatttg agcagattca tacaaagagg
    24181 gagagattcc ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa
    24241 cagtcatgat ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg
    24301 gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac attcggatca agcaacatag
    24361 aaaaaataat cgcaattgac gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac
    24421 aaaaacgatt gaatctggta tttttagata atgctactgc tgctaataaa ccaacgataa
    24481 tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa ttaactgctc
    24541 gttcatcact aaatatacgt gtgaaatttt caaaacttac aaaatttctt gatgtaaaga
    24601 aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg acagtaaata
    24661 ataataaaac taaaaaagat ggagttaaaa acaaatacgc agcacgatca ggacgattcc
    24721 aatatttggt gaatccgttc atatagtcca cctcatcttt cattttgata aaatatacaa
    24781 tcttggttta aacgaatctg aataagatat ccgaacaatc gctgtgattt ttgcaataac
    24841 acctatctgc aaatactagg tcgatcattc agatatctgt atcttgccaa cagtctatcc
    24901 tgtataaagt tctgtactat ttttgcagta attgattgat ggcatcatct gcatcgttca
    24961 tgagtgtctc aggatcttct cctgctaata atttttcaat cagtggattg atgatatcgt
    25021 tattgatcgc agagatttgg gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt
    25081 tcgataattc agatacaata ggatcatttt ttacttcttc atcttttgct actgaatcca
    25141 agtaggcagg aaaaccattt tcgatactcc attttttacc aatttcagtt gtattccaat
    25201 atttcaaaaa ctcatagaca gcttcttttt tattattatc tgtacttgcc ggaatggcat
    25261 atcctacgcc atctaaaata gctgtttttt tgtctgaatc actcccttgt ggtaaggtag
    25321 tcacattata atcaatctcg tttgctttca atccgttatt taaccatggc ccattgattt
    25381 caatggctag ttgaccagca ttcattaaat tgtccatttc ggcgccagaa atcgattctg
    25441 gaccagtctt gtctacatgg atcatttttt ggatcgcttt taacgtttta aggttctcag
    25501 gagacgcaaa ttcagactta gtataatcat cgttcactaa tttaccgcca tttgctaaca
    25561 tccagttata taacggtgca gttccatcct tattgaagac aaaacctgat acatttttac
    25621 tactatcagt taattttggt gccatttctg tcaattcttc ccaagtagta ggtggttgtt
    25681 cagtatctaa tccagcttgt ttaaacaaat cattattcca atacatatac atgccttgta
    25741 cctgcatggg gacaaaatac tgtttacctt ctacttcacc taattcaata gccgtttctt
    25801 taaaatcaga tttatctact ccatcatatt cccagaaatc gtctaatggt tgtaccgctc
    25861 cgttttctac atactgtgca aaatctggat aatttaatgc aataaagtca ggtgcttttt
    25921 tagaagaaat cgctgtagga agtttttcat tcaaattggc ccaagtcatg atatccattt
    25981 caattttgat gtttttatca tttgttttat taaattcatt aacgatttct tgcaatattt
    26041 caccatcaga tgcagtaaac ccattccaaa aagtcaaagt cacttcttta tcagaatcag
    26101 attctgctga acttccgcca ccgcaagctc ctaaaatacc tgttgcagct accaacaaag
    26161 agccaattaa ccatttttta cctttcattt tttgttcctc cttcatctga taaacgacat
    26221 ctgaccacat tccaagatag cggtttcaat aaacaattga ttttcccatc ttcagctttt
    26281 acatttgttg aatgacttgg ttttacttgt tcacttttag gaagattagt tgcttttgta
    26341 tcaaatccac tcatttcaga aaaatcaatc actccctcta atgataattc agacaagtga
    26401 acagttagat tcatttcttc ttctccacgg ttaactgcaa atatgacaag ttctttgtca
    26461 tttattacgg ctatactttc taaatacggc acttcttcaa aatcttccgt cgcatagttt
    26521 gctgaacgaa tttgtgggct taacacctct ccttgtccat acacagcagc ttgcataaat
    26581 ggataataga tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata
    26641 acatttacca attgagcaag acaagcaatc tttactctat ctgcatgttt caataaagtg
    26701 attaatagac agcctaatag taatgcatct tcaaaattat agttatcttc tagtaaagga
    26761 ggagcaatct gccaaggcgc tgcttgctca tcttgttccg ctgaatgata ccaaatattc
    26821 cattcatcaa aggacaggtt gatctgcttc ttactatgtt tcttcgcctt gactgtatcg
    26881 cacatcgctg tcacttcaga aatgaaccga tccatgtcta aggaacgtgc aagataatga
    26941 gaaagatcat ttttttggtt gccataatat tgatgcagag acaaataatc tatttgatcg
    27001 tacgcttgat caagaactgt taactcccaa tcaccaaatg taggcatttg ccgattcgaa
    27061 cttccgcata aaaccacctc aattgaatca tctacgagtt tcatcgcctt agccgtttcc
    27121 tctgctaacc gtccatattc ttccgctgtt ttatgtccaa tctgccaagg accgtccatt
    27181 tcattcccta aacaccatgt cttaattcca aaagggctct cttgaccatt cgaacgcctt
    27241 aaatcactcc aataagttcc tttaggaaaa ttacaatatt cgactaaatt acgagctgca
    27301 tctactccac gagtacccag attgacagcc atattgattt cggcccccac atgctgacac
    27361 cagttataaa attcatgaat cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc
    27421 aaccttctag gtctatttgc tatgggccca atactgtctt cccaacgaaa tcctgacaca
    27481 aaatttccac caggatatct aatcatggga atgttcaatt cttttaccaa gtgaatgacg
    27541 tcttggcgaa atccactttt attagctgat ggatgatctg gctgataaat cccttcatat
    27601 actgctcgtc ccatatgttc aataaatgaa ctgtacaacc gcggatcaac tttgcctaca
    27661 cgcttgacac tactaatcaa cattgtggct tccataactg acctccttta atttatacat
    27721 gaataataca cctcgttgaa agcgttgtca ataatataaa tgttaaactt acttattttt
    27781 tatcgatttt aattaaccaa aatattaata atgattaaaa caaataattt aatataactt
    27841 ttattaacaa atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact
    27901 acaaataaac gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa
    27961 gatcatccag ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg
    28021 agtgagcagt gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa
    28081 aacagaaaaa gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag
    28141 ttttccagaa aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca
    28201 ctatacaaat tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg
    28261 gaaagtggat gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg
    28321 gtttaccagc ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt
    28381 ggagatgttg gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg
    28441 gccttcagat atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg
    28501 agattttgct gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca
    28561 atacggcttg ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc
    28621 cttgtcaaac atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat
    28681 cgaagtgaaa gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg
    28741 taattacgat caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt
    28801 ttataaaaca aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt
    28861 tccagaaatc atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc
    28921 atgcttcgat tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa
    28981 tttcattata tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa
    29041 gatctgcagt cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt
    29101 gtttcccttg tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa
    29161 atttcgtgaa gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg
    29221 ctctttctcc agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt
    29281 tgttaaaatc gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag
    29341 ctggtttgat atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg
    29401 catcttcttc atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt
    29461 catcttttgt ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag
    29521 tagaatcttg gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca
    29581 aatatgcttt cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt
    29641 tgacacctaa ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc
    29701 tgtatgaagt tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca
    29761 gcattcagta gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc
    29821 ccatagtgaa tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc
    29881 gtaaactctt tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg
    29941 aacagtttta ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg
    30001 agggtcttca tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt
    30061 gatgcgccac acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt
    30121 gatcgtatcc atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc
    30181 aagggctggc tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt
    30241 agctttttgc atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat
    30301 ttttgcttcc gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc
    30361 catatctcca ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc
    30421 cacgcgaatc gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac
    30481 gcagtcttta tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg
    30541 gactaacgaa tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac
    30601 attgcttgtt gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc
    30661 cagttcttgt gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt
    30721 gatacgagct gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt
    30781 ttctactaat tggcagtgcg ctaaaggccc ttccacttgg attaatggtt catttgcaaa
    30841 aactagttct ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata
    30901 ctccaaaaaa ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt
    30961 gaacgtcaga ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc
    31021 gtggttaaaa ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc
    31081 taattcccag tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact
    31141 gtcgtctggg taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta
    31201 cccattataa caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac
    31261 atgttttacg gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta
    31321 ttggtattta acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa
    31381 gaaattagcg ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc
    31441 gtagccgtta agataccgat gattgaacaa atataaccaa atgtctctac aacataatca
    31501 atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga
    31561 aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata
    31621 ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa
    31681 gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg
    31741 ttttttattg tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta
    31801 ttaaatagaa tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt
    31861 gaattgtatt ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc
    31921 tcgtgtcatt tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt
    31981 cttcgtattg atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt
    32041 tcgatctaac gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac
    32101 tactttagga taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt
    32161 catctcattc ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc
    32221 tttcctagca ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt
    32281 tatcacttaa tagtataaca taatttcgtg agaaaaaaat acaatttttt cttgaccatc
    32341 tcgttacgta aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata
    32401 aagaaaatgt cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt
    32461 ggactgctaa aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa
    32521 gtcgatcgtc tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc
    32581 caagaggtct tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa
    32641 atgttgctgg aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt
    32701 gatatgtacg aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa
    32761 caaaagttga aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa
    32821 aaaaaggtta cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa
    32881 atgcaaactg ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa
    32941 gatgaaagct tagcagaccg tatcttccaa gctcataaga agtggttctc ttacagctgg
    33001 cctgaatatc agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg
    33061 ttcactgcat attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt
    33121 atccaaaaaa aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct
    33181 aataacaaaa cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca
    33241 ttctttcaca agttttttca tcctacttta aatatattca tcgttgaata aacgtataag
    33301 gtattttcac tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag
    33361 cttccagatg gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc
    33421 gtccaatcac cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca
    33481 tatccgaatt tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt
    33541 ttcctttctc tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa
    33601 gttgatgaca tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca
    33661 acgtgatgcc caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg
    33721 attgttctcg atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa
    33781 ctagaggtcc atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa
    33841 tgatcaatcc atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt
    33901 tatgatagcc cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga
    33961 tcccactgat caactgatca aaatacggat gattggtata aggcagaatc actccaatgc
    34021 ttttcgtttt tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt
    34081 tcatgaccgc aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta
    34141 cacgtgaaat agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca
    34201 ctgctgcttc gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc
    34261 gcattcaaaa aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca
    34321 atatatatct tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct
    34381 attcaaccct tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata
    34441 aaaataattg ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc
    34501 ttttgatact ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca
    34561 aagaaaagtt ttattcctct ttcattatta ccaaagtctc ttatt
//
LOCUS       DACIRY010000017        34545 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.017, whole genome shotgun sequence.
ACCESSION   DACIRY010000017 DACIRY010000000
VERSION     DACIRY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.017"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            139..423
                     /locus_tag="IYE22_000684"
     CDS             139..423
                     /locus_tag="IYE22_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ9505917.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(704..1123)
                     /locus_tag="IYE22_000685"
     CDS             complement(704..1123)
                     /locus_tag="IYE22_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ9505918.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1125..1709)
                     /locus_tag="IYE22_000686"
     CDS             complement(1125..1709)
                     /locus_tag="IYE22_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ9505919.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1756..3231)
                     /locus_tag="IYE22_000687"
     CDS             complement(1756..3231)
                     /locus_tag="IYE22_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ9505920.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3412..3723
                     /locus_tag="IYE22_000688"
     CDS             3412..3723
                     /locus_tag="IYE22_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505921.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3838..6174)
                     /locus_tag="IYE22_000689"
     CDS             complement(3838..6174)
                     /locus_tag="IYE22_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9505922.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6316..6984
                     /locus_tag="IYE22_000690"
     CDS             6316..6984
                     /locus_tag="IYE22_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9505923.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6987..8030
                     /locus_tag="IYE22_000691"
     CDS             6987..8030
                     /locus_tag="IYE22_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9505924.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8267..9148)
                     /locus_tag="IYE22_000692"
     CDS             complement(8267..9148)
                     /locus_tag="IYE22_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9505925.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9307..10035
                     /locus_tag="IYE22_000693"
     CDS             9307..10035
                     /locus_tag="IYE22_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ9505926.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKSCGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10191..10508
                     /locus_tag="IYE22_000694"
     CDS             10191..10508
                     /locus_tag="IYE22_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9505927.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10501..11109
                     /locus_tag="IYE22_000695"
     CDS             10501..11109
                     /locus_tag="IYE22_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ9505928.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11125..11955
                     /locus_tag="IYE22_000696"
     CDS             11125..11955
                     /locus_tag="IYE22_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ9505929.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12093..12581
                     /locus_tag="IYE22_000697"
     CDS             12093..12581
                     /locus_tag="IYE22_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ9505930.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12632..13504
                     /locus_tag="IYE22_000698"
     CDS             12632..13504
                     /locus_tag="IYE22_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ9505931.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13680..14042
                     /locus_tag="IYE22_000699"
     CDS             13680..14042
                     /locus_tag="IYE22_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ9505932.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYIQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14051..14803
                     /locus_tag="IYE22_000700"
     CDS             14051..14803
                     /locus_tag="IYE22_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505933.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14817..16619
                     /locus_tag="IYE22_000701"
     CDS             14817..16619
                     /locus_tag="IYE22_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9505934.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16896..17936
                     /locus_tag="IYE22_000702"
     CDS             16896..17936
                     /locus_tag="IYE22_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ9505935.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17988..20132)
                     /locus_tag="IYE22_000703"
     CDS             complement(17988..20132)
                     /locus_tag="IYE22_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ9505936.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20489..21862)
                     /locus_tag="IYE22_000704"
     CDS             complement(20489..21862)
                     /locus_tag="IYE22_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9505937.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21862..22257)
                     /gene="rbsD"
                     /locus_tag="IYE22_000705"
     CDS             complement(21862..22257)
                     /gene="rbsD"
                     /locus_tag="IYE22_000705"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ9505938.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22285..23202)
                     /gene="rbsK"
                     /locus_tag="IYE22_000706"
     CDS             complement(22285..23202)
                     /gene="rbsK"
                     /locus_tag="IYE22_000706"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ9505939.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23310..24323)
                     /locus_tag="IYE22_000707"
     CDS             complement(23310..24323)
                     /locus_tag="IYE22_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9505940.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24404..25003)
                     /locus_tag="IYE22_000708"
     CDS             complement(24404..25003)
                     /locus_tag="IYE22_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9505941.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25189..25890
                     /locus_tag="IYE22_000709"
     CDS             25189..25890
                     /locus_tag="IYE22_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9505942.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25900..28611
                     /locus_tag="IYE22_000710"
     CDS             25900..28611
                     /locus_tag="IYE22_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ9505943.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29062..30690
                     /gene="treC"
                     /locus_tag="IYE22_000711"
     CDS             29062..30690
                     /gene="treC"
                     /locus_tag="IYE22_000711"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ9505944.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30750..31931)
                     /locus_tag="IYE22_000712"
     CDS             complement(30750..31931)
                     /locus_tag="IYE22_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9505945.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32183..33706
                     /locus_tag="IYE22_000713"
     CDS             32183..33706
                     /locus_tag="IYE22_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ9505946.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33775..34503)
                     /locus_tag="IYE22_000714"
     CDS             complement(33775..34503)
                     /locus_tag="IYE22_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9505947.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT        10916 a         6180 c         7009 g        10440 t
ORIGIN      
        1 tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt cgaaaaaagg
       61 aactgcgaca agacttttgt cacagttcct tctttttttt gctatgctat actagatgta
      121 aaaaatagga gaaataaaat gagggaaatt tattcttttg aatcaaaaat ctatgcttcc
      181 gaagtcggca agggcggggc atatattatt tttccgtatg atataagaaa agaattcggt
      241 gctggaagag ttaaagtcca agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg
      301 aatatgggcg tgaaaaatcc agatggttca gtctgctaca tccttggact acgaaaagat
      361 attcgtaaaa acatcggaaa agaaatcggt gatattgtag cagttacagt gaagcaaaaa
      421 taaaagaggt tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt tcaaaagcca
      481 gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa acccatgttt acaaaatttt
      541 tcttactact cgaaacgaag cggctttatc acaacctctt gataaacggc gttcaatcag
      601 ctgtccctcg gtattagctc aaaaacggca aaatatgaga agctattttt ccattttttt
      661 ctctcatact caggtcataa cgctgatttc acaacctctt ctttcataat ggtttttcac
      721 catctttata ataaactggt tctttgatat ggaaatctat tttttccaac caatctaaaa
      781 tcttgtaacc taactcttct gacatcggaa aagaggcgtt gatttgtgca ttggagatat
      841 ggtcaacatg gataccggaa gtgatcgtac aacttcccgg aacggcagtt ttgatgatca
      901 cagctatttt ttcggctaag acatcgtctt tatggaaccg accatcgtgg ctagggaaac
      961 gtacagtttg catctctgta tcacgagaaa atgtagtgac tgttccgata tgtggtgtat
     1021 ctccgcctgt taaagtgatc aataagtctt taccgattac tttaacagca gcttggatcg
     1081 tgaaacctag tttggttact tcaactatcg tttctttttc catattaatc accctgccaa
     1141 cgttttgaat gctcatttac aatttcaaat tgatccaaaa gtttcattgt atggtgatta
     1201 acaagatctt ggatcgtttg aggctgatta taaaaagcag gaattggtgg tatgatttgc
     1261 acacctagtc gtgctaattt agtcaagttt tccaagtgaa tggcatttaa tggcgtttca
     1321 cgaggaacaa tgactaactt tcgttgctcc ttcattgtta catcagcagc acgtgcaatc
     1381 aggttgtcgc ctaaaccaac agagatacct gcgactgttt tcatacttgc cggtacgatg
     1441 atcattccat cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc tttgtttgaa
     1501 taatggtaat caaacaggct ttcgaattca gttagtgata agtcggattc cagctttaag
     1561 ttctgttttg cccatggact gatgattccg tgtgtttcta tcatcggata ttcttttagt
     1621 ttcttcacta ggttgatggc ataaatggtg cctgaagcgc cacttacacc aatcacgatt
     1681 tttttcttcc gttgtgccaa ttgttccatt ttccgcactc ctttttatga tgtcaaaaca
     1741 gttgcagatc attcattatt tcaaatattt ttcccaatct tctacttctt ggaattttgc
     1801 acgtacaaaa tgttctttca tatcgaaagg aactgtaccg tcaaagatag tttttgcact
     1861 tatcccacgg aaacgaatag agccaggatt atattctggt aattctgatg ggtccaatgg
     1921 atggttcctc attcctggga tcacgatgat atctttatct ccttggaaac gagtgttgaa
     1981 agtccaaact acatcgttca tgtcgaaaat atcaacatct tcatcgacta aaatgactgt
     2041 cttcaattct ttgaaggctg ataaagcaag aatagctgct tgacgctgaa taccttcatc
     2101 cgcttcgttt tctttacgga tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg
     2161 attgtagaca tttactactt ttcctggaat agctttatcg accaaggcaa gaatacttgc
     2221 ttctgtagga ataccggcca tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat
     2281 gattggattg tcttttcggt gagtaactgc tttgactttc actacttgta cagctggatt
     2341 agcatcccca gtatagccag gaaattctgg cattgctttt cctgagttcg tgttgatatc
     2401 ttcttgcatt gtttcatttg gcatgatttc tgcttcaatg atgaattcag cacgagcaat
     2461 acccacttca tcgactgcta taccatcaac gatttgaata ggttcgttac gcaaagctcc
     2521 tgcacaccat agttcattgt agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat
     2581 ggcaattgct ggatctaaac caatattgat agtgataggc attggtttat tcaaggcttc
     2641 gaattgtttt tggaagtgac caatgtggcg tccgccaggc atgatgtaca taccgatcgt
     2701 atctttatct tctagaacca tacgatggat cgttacatcg ctcattgttt tttctggatc
     2761 tgatcctaag actacaccca ttgtgatgta tggaccagca tcttcaggag tatttgtagg
     2821 tgcaggcaaa atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa cgacttcttg
     2881 tgctggagcg tctgctttgt ctactttcac aggtttgatt ggattttcta cagcgtcttt
     2941 caagaaacga ccaagtgttc gataatcatg gtgcaaaatt tcacctacac gtttacgact
     3001 agccattgtt ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat
     3061 agctggtcct tcttgagtcg gacgttttac tgtaccgcca gcaccgatat aacggtaaac
     3121 accagataat tctgcttcag gatcgatttc aatatctgtt tcgtgatatt gacctggaag
     3181 tgtttttagt tcttcgatga cttttcttaa atcatacggt tgatctgtca tgagagattc
     3241 ctccttagtt gtctttacct atctatagta aaaggtgaaa aagaggaaaa caattcaact
     3301 tgttcggtaa agtattctac aatggaatgg atgcgttttt ttgataatag aataaactat
     3361 gatatactta tcactaaaag aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa
     3421 atggaggagt catttcctaa agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt
     3481 acatttgaaa acggagaagt aaaatacgtg aaatctcatt ggacagaaga gataaccgat
     3541 gccttgcaat ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc actttctcgt
     3601 aatatgtgga ttgggactga agtaacgatt gaagcagatg gaacagtatt cataaatgga
     3661 aaagacaggt atacgccaga agagttgtgg tacaaaggaa agaaaagtat accagaatta
     3721 taaaaaataa ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct cgtttcttgc
     3781 tgttttctac tatttcagcg ctcttatgac tcaaatctaa atgtggttgt cacaacctta
     3841 ttctgcacgc aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc
     3901 gccaagcatc gtcaatatag tagagacgat cactagaata atcgcatgga caggatttag
     3961 ttgagcaaca ttttttagat ccgtcatgtt gtataagatg ctattgatag ggaacgtagc
     4021 tagccatgca ataaacacac ctaatatccc agaagaaata cctaaaatac acgtttctgc
     4081 gtcaaatacg cgagtgatgt cttttttacg tgcacctaaa gcttttagta caccgatttc
     4141 ttttgtccgt tcaatcacag atgtataagt aatgatactg atcatgatca tacttgttac
     4201 taaagagatc cctgcaaaag caataaggac ataagtgata gcatccatca atccgccagt
     4261 cagttcagtc atcgttccag ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc
     4321 tttattgtaa tcatctagat aatccaagat cttttctttg tcttcaaaat tgttcggata
     4381 aatcatgata cttgaaggca agctgtctcc acctaaataa ctaagcaatg tttgtttggc
     4441 attttcgtca actttttcag tagttaagac atttacacca ctgtctcttt gagctttgac
     4501 gatttctgat tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc tataagcgat
     4561 tcctggagat aagagattca ttgtagaaga agatttcacg cgtaggatcc ccgagatctt
     4621 caattcatca gaagcattct gatacatcgc atcataatct gtattaggga taaaattccc
     4681 agttggaagt tttgtatagt aagtattgtt gtttactaat ttgaatgttg taccaacgat
     4741 atcatcaaaa tcaagttttt cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt
     4801 gatattagta ttattattgc cgtctacgat cagcacgaca tctgttgctg aggctggata
     4861 acttccggca agtaaagaat aattgtcttt taaaaagttt tctttgctag tgtcaagctg
     4921 tgtagggaaa cttgacactc ccacacctgt cattgctgac atcgtactag ataaagaaag
     4981 agattccgta tcaggatttt gattcgagaa gctgacaggc tgtactttgc catttacatc
     5041 acgtaaaaga ttgattcctg tagtacgggt aaatccgata ttattactta agttaggatc
     5101 gatgtctgtt acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc
     5161 ttcttcgctg acttttgcag taatcgtttt gctgtctgga taatccgctt tgcttgcacc
     5221 caatccggca tcgtcacgag tctggttagt cgttacctta gaaatagtaa tcggaaattt
     5281 cgccatcgtt tcagcttgcg tgttatcgat ttgtttttgg aatccagaag aaagggacag
     5341 aacgatcgcg attccgatga tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc
     5401 tttttttgtt cggatattgt tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt
     5461 tctgcgtaag ttaaattgat catcttttgg tcgttcgatg tgaggatggg aatctgttaa
     5521 gatcttacca tcagaaaatt caatgattcg atctgcgtat tgatgggcca gttcaggatt
     5581 atgtgtgacc atgatcacta atttttcttt cgataactct tggatcaatt ccatgatctg
     5641 gatacttgtt tctgtatcta acgctccggt tggttcatcg cacaaaagga tatctgggtc
     5701 attggctagt gctcgagcga tggccactcg ctgcatttgt ccaccagata actgatttgg
     5761 ctttttatgc atatgatttg tcaagccgac tcggtgcaat gcatcttctg cccgttttcg
     5821 tttttcgtct ttagaaacac cacttaatgt catcccaagt tcaacgtttt caataatgcc
     5881 tagatgacca atcaagttgt aactttgaaa aatgaaaccg atagagttgt ttcggtaggc
     5941 atcccagtca ctgtccttaa agtctttagt agactttccg ttgattacca tatcaccgga
     6001 atcataatta tctagtccac caatcacatt cagcatagtc gttttaccag aaccactagg
     6061 acctaaaatc gctacgaatt ctttttgtct gaaagcgaca gacacaccat ccaatgcttt
     6121 ggtggtggtt tcgccgactt tataatattt ttttatatct tttaattgaa gcataatcac
     6181 ccttctcccg tattgaaata ttcggtatac taataagtat atcagaacaa tgatcgtagt
     6241 atacgaaata aatgttaact gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa
     6301 aacagaggga gatcaatgaa tcgtatttta gtagtagaag atgatccgca attacagttg
     6361 ctttaccgtt ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt gaatggagag
     6421 gatgcgctga aaaaactaga agcgacacgt atagaattaa tcattacaga tatcatgatg
     6481 ccgaaaatgg atggctatga cttgctagac tcattacgta gcaccagatt tgacacacct
     6541 gttctaatga ttacggcaaa ggccgatttt gaggataaga aacgcgggtt caaattagga
     6601 gcagatgatt atatgacaaa acccatcgat gtcaatgaga tgatcctacg tgtggaagca
     6661 ttgcttcgac gttcaaagat caattacagc agtagcttga taatcggaca aacaaaatta
     6721 gagctagatg cttatacagt gttacaagat cagcattcgt tgattctgcc tcaaaaagaa
     6781 tttctgcttc tgtataaatt actgtcttat cccaacaaga tcttcactcg ccaacagttg
     6841 atggatgata tttggggatt agatacgaat acagaagaac gaacgatcga tgtacatatc
     6901 aaacgtttaa ggacccgttt tgaaaagacg gaagattttc aaatcatcac agtacgggga
     6961 ctaggctata aggcggttat ttaaagatga aaaaaaagct attttcacaa ttatggatct
     7021 attttgctag tattgtcttc atttcgatct tagtgacact gctgtgtttt ctcggtttta
     7081 ttttcttatt gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat
     7141 acccgttatt cgtctttgcg gggttcagta tggtcgtggg tacaggaata tcgatttttg
     7201 ttgggcggcg tatcctgcat ccaatcagtg cgctagggac aaatatgagt ctggtagcaa
     7261 cgggagattt ttcgattcga atggatgaac agcagaaagt agcagaagta cagcaactgt
     7321 ataaagattt caacgttatg gtccaagaac tcaatagtat cgagacactg aggaatgact
     7381 ttgtgtccag tgtatcccat gaattcaaaa caccactagc aacaatccaa ggatatgttc
     7441 aattgttaca agccccaaat ctctctgacg aagaacgcca aatattttta tatcggatta
     7501 tcgaaagtat cacgcagctt tcacaattaa cagagaacat cttaaaattg aacaaactgg
     7561 aaaaccagcg tatccaactg gaaaagaaag agtatcgatt agatgaacaa atcagagaag
     7621 taatcgtttt ttttcagcct aaatgggaga aagaacagct agaattggat atcgaacttg
     7681 ctgcagtaaa ttacacaggc aatgaagagt ttttgtacca agtgtggctg aatatcatgg
     7741 ataacgccat aaagtacaat caaatcaatg gacagattca tatcaaacta tttgagacag
     7801 ccacagagat tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa gagaccagag
     7861 atcgaatgtt tgaaaaattt tatcaaggag atacaagtcg tcaaatttct gggaatgggc
     7921 taggactttc actggttaaa aaaattctag aactgcatga tggacgtatt gattatagca
     7981 gtatcgaagg agtaggaacg acagcaatga tacggttaag taaacaatag gaaaaagaat
     8041 aaaaccccta aaaattctat tgaaggtttt taggggtttt tagtcgtttt tttgtgatta
     8101 gggaaatgtt cttacggttc ttttggcata aaaatcgctg ctttcttatt gaactacgag
     8161 aaatgttaga ggtgtcattg aaacaaggac atgatgcaag aaagagagcg aaagtcatgc
     8221 gtcgctctct ttttttgggt ggatagatgg acagaaaatg tagggcttag ttttcttctt
     8281 gatttaaata ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa
     8341 atgcaagata actctctgtt gttggagaaa tagggagaat cacagtattt tcttttaata
     8401 gattcttgac tgttttttcc ataacaatag caatccccat gtcatttgca atcatattga
     8461 tgatcaagtc gattcttgct cctttataag agatttttgg ttcaaatcca gcttcgcgac
     8521 acagatctag tacaggttga tacaagttcg tagtggggcc aaggagcaaa aagttctcct
     8581 ttttcagttc gcttagatcc aatttctttt tactagctaa aggatgattt ttaggtagaa
     8641 ctgctacaaa atcgtctgtt tcagtaggca accactcaat cgaacttggt ggggcctgga
     8701 aacttctagc aaagtggatc gtgtcactat gttcattggc gtcattgtaa gttaattcga
     8761 cactttccac ttctttcaac tggactgtaa attcaggatg gcgtttcaaa aactcggtga
     8821 ttttggaaaa acctttataa ttgatcattg tcggtatcgt taagatattt aatgaaagat
     8881 tgttgtcttt cgtataggtt tcaagcactt cttgcatagc atggtattgt tctacgacat
     8941 tttttacata tgggagcaat aagcttccag cttcggtcaa ttcgattttt ctatgggcgc
     9001 gttcgaataa tttgacgtct aattctgttt ctaacgaaag aatctgttta gaaatatttc
     9061 cttgggtggt gaattgtcga tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa
     9121 gaaaaacttc gaatttatgg atatccatca tattcctcct acttattcca ataaagaata
     9181 ctttaacaca aaaaatgaat tggtgtaatc ttctctgtct gttacaatct ttcttgcagc
     9241 gaaagcaata attacagaaa tttttctgat ttttttcgct tcgtagaaaa agagaggagt
     9301 tttttgatgc caactggagt tttgatcaat gtaggttccg ttttgtttgg cggactaatt
     9361 ggcggattag taggaaataa attaagtgaa cattttaaag cacagctgac gatggttttt
     9421 ggtgtgtgtt cgatggggat gggaatctat tccattgcgc cgatgaaaaa tatgccggct
     9481 gttatttttg ctttagtgat aggaacagcg attggtttga ttgttcattt aggtaatggg
     9541 ataaataagg gggctgcgct gatgcaagta cctatttcta aaatcttccc tagtgaaaag
     9601 cttggtatga cccacgatga atttatcagc acattagtga cggtcattgt tctattttgt
     9661 gcaagtggta cggggatcta tggaagttta gattctggga tgacaggaga cagtacgatt
     9721 ttgatttcta aatctgtcct tgattttttc acagcagcta tttttgcgtg taatttaggg
     9781 tatgtcgttt ctgtgatagc aattccccaa ttcattattt tttatatttt gttcttattg
     9841 gcaaaattta tttatccatt gactacacca gacatgatct tagattttaa atcttgcggc
     9901 ggattcttga tggtcgctac cggttttaga atgatcaacg tgaaaatgtt cccagtagct
     9961 gacatgatac ctgcaatgat cgttatcatg ccactgagct ggatgtggac aaattggatt
    10021 atgccactgt tataataaaa atcaaagaga agccattttt tgctgactaa aaaggatcaa
    10081 atcttttagt cagttttttt gtatttgaca aactgatttt gaaactatat tatatgttgt
    10141 ataatataga ttgaaataaa atattacact tgagaataga aggtgacgta atgcacatcc
    10201 agattccaac tgttcttctt gatggtactg tgctagcagc attaagcaag gaagatatgt
    10261 atgggtatgc cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa tctacactat
    10321 atccggtttt gagaagattg aaaaaagaag gatatcttga gacctatgat gaaccttttg
    10381 aaggcagaaa ccgtcgatat tactcgttga caccaatggg aagtcgtcat ttagaagaaa
    10441 ttattgatga atggattgtt tttcgagatt ctttagaccg gttattggag gagagcacaa
    10501 atgaatgaat atttgaatga agttgtggat catcttcaag aaattcctga agacgatcgt
    10561 tttgatttga tccagtatta tgaggaatat tttttagatt ctggaaaaac attagatcag
    10621 atcattgatg aatatggtac accaaagcag tttgccttaa aattaaaaat cagttacttt
    10681 tcagaagaag aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg
    10741 atttggttaa tcattatcgg tttatttgct tctcctgtcc tcattccact agcactgggg
    10801 tttatattgg ttattggggg actattggtt gctttactag cgctcatttt tagtatctat
    10861 gttttatgta tcagtatctt aggtgttggc ctattcatga tcatctcagg aatcagtgtg
    10921 tttggacaat cgattctgag tggattattc tttatcggtg tcggtctgtt cgccacagga
    10981 agcgcagtat ttatttcgcc actgttgctg aaaggaacaa aagggctttt ttatgtcatg
    11041 atgaactttg ttaaatggat gggacgacgt tttattacaa aaagagatct gcattcagcg
    11101 ggcgtttgaa ggaggacaca taaattgaga ttgaaacatt ttttgattac cggtattggt
    11161 ctgatgctta ctggtggcct cctagcaggt gcaacttata cattaggcgc tcaaaagagt
    11221 ctgacttggg ataatggtcc tcggttgatc gatatggtta ctgagacaaa aaaaatcaag
    11281 gatgcaaaca aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat acgcggattg
    11341 gattttgaga taaaaacgac ttatgataag acagaaaagc cagaaataaa ggagaaaaat
    11401 ggcgtgttga ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag tttagaagac
    11461 acaagtgcac aaatcactat tgttgtccct gcagatcatg aattatctga attgacaatc
    11521 gaagggaata atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag
    11581 acacatgatg gctgggtatc gttcaatgaa tttacaggag gaacaataac tgcgaaagat
    11641 gaattaggct cacttcgatt agatgaggcc aatgtggata acttgatcgt cacaggtgaa
    11701 aacagcaact tccatctaga agacagtttg atcagcaaag aaggaaaagt catcctaaac
    11761 ggtggcaatt tatctttgca tgagatggaa aacagtggct atactatcca aacgtctggt
    11821 gaatcttata ttgaaaaaga ctaccagcca ataacaaata ctttgttaga acaaggacag
    11881 aaaaagctaa agatcactgc taaagacaca tcgatcaata ttacgacgga tcaagaggac
    11941 aatgaaacag attgatcctg tactcaactg gatattttaa gaaaaaacag caaagatcct
    12001 tccaatcttt gctgtttttc atcgcttaac gcacataact atagtaaaat aagaaagaaa
    12061 atatctaaaa agaaaacagg gtgaaagagt atatgaaggt agaagaaggt caaagagaaa
    12121 agttaaaaac agaaatggaa agattacata cttatatcac tcaactaagc caaaccttct
    12181 atgatccaga caaagaaaaa gtgatggtta attacccgaa caattcagaa ggtcgccaat
    12241 tagagcaagt gtaccatgaa gttttcaaac atttattgac ggtgaagaaa gagctggatt
    12301 attattcttt accgattatc gatacaggaa tattgaagta tgatgggaag aaggaacgat
    12361 ttatctttaa atctgttcgg gaagatttgc ctctttcagc agggatggat ctagaggtct
    12421 tggtagaaga ctattttaca gaagaaaagc actgggttcg aacaaaattg gattatttac
    12481 cgcaggcagc cggagggaca caggcaagcg gttggtacat cactgaagac aaagagttag
    12541 aactagaagg tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac attagatatt
    12601 tattgaagga taaaggagcg caggaagtag catgagagaa aaaagagcat tttatatcaa
    12661 tggttatgca gggattgtcg gattggtcgt attagcttta gttggattgt ttttcttcta
    12721 tttaggtatg tggcaagcga aagtttgggc acttttttta agtatttttc tttggttgat
    12781 cacgttattg ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa aagtaatttt
    12841 gttttttgga caatatttag gtacgattcg agaaaatggc tttttcctta cgataccttt
    12901 ggcacaaaaa atgactgttt cgttgaaagt tcggaatttc aatagttctg ttttaaaagt
    12961 caatgatttg gatggaaacc ccatcgaaat ctcagcagta gtagtcttta aagtcgttga
    13021 tacagcaaag gcattattcg atgtagcgta ttatcaagat tttgtagaaa tccaaagtga
    13081 gacagccatt cggcatatcg ctagccaata cccgtatgat acattcaatg agaatgattt
    13141 gacgcttcga ggaaacacga cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg
    13201 tttagcagta gcaggtgtcg aagtaatcga aacacgcttg aaccatttag cctatgcaac
    13261 agaaatcgct agtgccatgc tccaacgtca acaagcaaaa gccattttgt ctgctcgaca
    13321 aaccattgtt gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg
    13381 acaagaaatc aatttcaccg atgatcggaa agtccaattg atcaataatt tacttgtctc
    13441 aatcattaca gacaaaggaa cgcaacctgt tatcaataca ggcgacatga aagaccgtaa
    13501 gtgacatact ttttgtaaga taggaagaag gagtttagga cggattattg tcttgaactc
    13561 tttttttagt tgttttaaag aaaatgacaa aattgaaaga agaatgatag ttgattcgaa
    13621 ggcagaattg ttcgcttttt tctataatca gtatataaag taagagagaa ggggaaaaag
    13681 tgaaaaaact tctgatcggt ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa
    13741 tcgcagatta cgtagttatg ggtggagaag aatactatat acaaattacg acagatggta
    13801 caaaaacagt ttcaaaatca gactctggac aagaaacagt caactataga tatatcctgc
    13861 ctggctatac gaaagagggt aaggaaaaag aactggactt caagggacaa ttgactcgac
    13921 cgcttcgtaa aggagcatat ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt
    13981 atgaagaagt cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta attaaagggt
    14041 agggaaagaa atgaacaaaa tagtcgaagt acagaatgta acaaaagtat acggaaaaaa
    14101 caatgagaaa aagacgcaag cattaagcgg catcagcttc gatgtagaaa aaggcgaatt
    14161 tattgggatc atgggcgctt ctggttctgg taagtcgaca cttttgaata ttttatccac
    14221 attagataaa ccaacagatg ggcatatccg aatcaatcaa aaagatgtga ccacattaaa
    14281 aggaaatcaa ctagcagatt ttcgtgcgaa tgagattggc tttattttcc aagatttcaa
    14341 tttattagaa agcttgacag cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt
    14401 cagaccaaaa gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat ccatctctca
    14461 tattttggaa agctatcctt ctgagatttc cggtggacaa aaacaacggg tcgccgccgc
    14521 acgtgcattg atcacacaac caacgattct attaggggat gagcctacag gtgcgctgga
    14581 ttccaaaagt gcccgggatc ttttagatac gatggacgaa ttgaatacaa aagatcatgt
    14641 atcgattctt cttgtgacgc atgatccgtt ttctgcaagt tattgccaac gtattttatt
    14701 catcaaggat ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt
    14761 ctatcgagaa atactatcta ttcttggaaa cttggaacag tagggggaga ttgactatgc
    14821 tttggaaatt atcacttact gggatcaaga gccgtttaag agactacttg gtgttattct
    14881 caggattagt tatggcatct gctatttttt acatgtttga atcaatggca agcaacgaag
    14941 cttttttgaa aagtaatacg atcgttgcgg ctacagttat cattttccga ttcggttctg
    15001 ttttactagg gatcatcaca ttcgtttata ttttatatgc aaattccttt ttgatgacga
    15061 tgcggcaaaa agactatgca atgtttatga tgctgggagc aaaaggcaga aaaatcgcac
    15121 agatgatttt ttctgaaaca ttcttagtag ggatcgcagc tactttagtg ggttcagctg
    15181 tcggtgtagg attgacaagt atcgtccacc gattattagt ggatcagtta gatctcaata
    15241 tttcgcattt tacacctttt agtattcacg ggttgttgat cactgttttg ttttttgctg
    15301 ttttgttttt attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag
    15361 cattgattcg tgcggatcag acaccaacaa gaatcaagca gcataaattc ttgtttttcc
    15421 ttgaagtagt actgggaatt gcttttctag gctgcggtta ctatatgatg aaacacgtgg
    15481 ggacctatca agtctttggt ataggggtag ccttagttac gatcgtatta ggaacttatt
    15541 tcattttcca ttctgtgatc attttctttt tatcattact aaagaaatca gaacagattt
    15601 cattgaaaaa attgaacaat ttcacattgt cacagttaaa ctttcgtatt cgtgaatata
    15661 cacaaatgct ctcaatggtc gctatgctgt ttgccttggc actgggcgct ttgacggtag
    15721 gattaggttt taaaaatgaa attgcagact tgacagaaaa caattctgga tatgatctag
    15781 tgatgaataa tcctcgggca gaagatcagc aaaaaatcaa agaactttca ccaacacttt
    15841 ctgcttctta tactcaaaaa gaagatgcgg gaacgattta ctataatgca agtgaatttg
    15901 cggctcaacc attgatcatg ataaaacatg aagatggaac accgccgaag atcacgaaag
    15961 aaaaagttcc tgctaaaaaa atgaatgaac tgactgtaca agaggaactt cgaagctatg
    16021 aattgccgga acagcaagaa aaagaaatca aggtagtcag tcaatcagaa tttgatcaac
    16081 tgaacttgcc acaatccact ttacagctag ttcaggtaaa agacttccaa gcaaacctga
    16141 aaccaatcga aacacttgtt gctcaaaata aaatgaacaa tccatctttg caaaaagtag
    16201 agtacagcaa ccagaaatat gaaatgtatg aagtgtataa tggtctattc tctggttttg
    16261 aatttatggg attcttccta ggtttagctt tcttgaccat gcttgctagc tgtttgatgt
    16321 tcaagatctt gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag
    16381 gaacacgacg cgggttgctt aaacaatcga tccgtagaga agtaggcgtg ttattcttag
    16441 caccaggaat cttaggtgta gtccatgtct tattcggatt acaaatgttt ggtctattca
    16501 tgcaaaatcc atacaaagat atttgggttc cgtttacgat attcttcgta ttgtatttca
    16561 tctactatgt actgacgaca tggttatata ctggaatcgt attgaaaaag agattgtgaa
    16621 aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa aatagctcct catatttttc
    16681 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga
    16741 ggttgtgaca atatttttgt cacaaccttt ttttcacggt cttttattag aatatagtaa
    16801 attttttctc gttactcacg gaataagctg ttaagaaaac ggtttccatg tatactggaa
    16861 tcagtgcaaa tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa caaaattttg
    16921 gcgaaaacta tcacttgatt acgatcacga atcaaaatgg gacatctttg tctgtcagtg
    16981 atcttggggc acgggttgtt cattttcaaa caataatcga taatgaaaaa cgagagttga
    17041 tcttaggttt tgatgaagca aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa
    17101 tcggacgtat ggctggacgg attacgaatg gcacattcaa attagatgat gaaacgttcg
    17161 aagtaaaaac agacccggac actggtcatt gtttacatgg tggtgcacct agctttgaag
    17221 ccaagcagtg gcattataca gtcatcaatg gtgaaaacga agcatcggtc attttctatc
    17281 agactagccc agatatggaa aacggatttc ctggaacttt agatgtagaa gtacggtata
    17341 cgctgacgaa cgatgataca tggcgagtga cggtccaagg gatcagtgac cgcaaaacag
    17401 tattcaatcc tacaaatcat gtctatttca atttaaccgg tgatccagca cagtcaatcg
    17461 acgaacatat actttggttg aatagccgat tatttgctac gctcaataaa gatacaacgc
    17521 ctacaggaga agtagtttca gtaagtggca cagcatttga cttccaacgt cctaaaaaat
    17581 taggtgaggt ctttgccagt gattttcgcc aatcaatgat agtgggcggc atcgatcatc
    17641 catttttgtt gaaaaaacaa tctggtttac taaatgctgc ggaattgaca agcccagatg
    17701 aaaaagtatc gatccaagta aaaacagatg cttcaggaat cgtgattttt acagcaaatt
    17761 ttggagaaga tggaccggaa atgcgcggga ataaattagt aaatcatggc ggtatcacat
    17821 ttgagactca agaactgcca ggagcggagc gacttgaaag ttttggagat gtcactctgg
    17881 aacctaacca agtacgcgag tttgtaacgg aatacaaaat aaatataaag aaatagaata
    17941 aattaaaaag ggagtgtgac agtgtcacat ccccttttta atttattcta tttctttaaa
    18001 ggcagatcaa gccagaatga tttgtctaaa cttaacaagg ttagtacagg aacagttgca
    18061 gctttaactg tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt gttccacaaa
    18121 tcccagattg aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt tatcgttgaa
    18181 aattctaatc ctgtgattgg cacgccatct gttgtccatt tgattggcac atcgaatcgc
    18241 ttagacatat tgaggatttc agcgtttgct tcttcagttg ttgctgcttc ttttttcggt
    18301 tcatcatatt ttgtcaggtc gtacgcttcg atgatcgcgt tcaatttttg tgaatattgg
    18361 cgatctgtag cataacgtcc ttccaaatat ttggttgctt ctttgtagtc ttttgtttcc
    18421 gacttccaag ttggtttgta gaaatcagga ttgccagtca gtccattttt gatcaagtcg
    18481 gcatagtctt ccaaactttc cttgtaagaa ggatatttac ggaattgtgc gcggatttgg
    18541 tagctttgtc cggaactatc ttgttcaagt gtattgaagg tcacgctgct tcctttatat
    18601 gatcctttga taccaaaaag gttatgattt ggttcgcttg ataataagct gtttccagat
    18661 ccactttcaa ggatagcttg agccatcatg actgatgcgt agattccttc tttttgcccg
    18721 atttcctgtg catcatctgc aatctttttc aagaactgat cagttgtttg gttttgactg
    18781 aaatggaacg agccatctaa tccatctaaa atagatgagc cgcctgtcag cgaacctgaa
    18841 gcagcaggta aacctgctaa gtttactcgg acagcaaaat caggcgtgaa atctgaaata
    18901 gaagttactc gaactttatc tcctggttga ggcgcatgga taaattgtcc gttcccaata
    18961 tagataccta catggtgagt aacaccggaa gaaggtcccc agaagacgag gtcgccaggt
    19021 tgggcctgac ttactggaat ctgcgtacct gcatattgct gttcgcctgt ccagccaccg
    19081 atataatgac cggttgcttt catatatacg taataagtca aaccagaaca gtcaaaagta
    19141 tttggacctt ttgctcccca tacatatggt tttccaagat gcttgaatgc ttctcgaacg
    19201 attgcttgtt ggacattcgt tgtatttgta ggcaatacag catcaattgg tgattttaca
    19261 acaaaatcag aagaagcaga agatggattc tctataccgc tttgatttgc tgtatttgga
    19321 cgagatgaag cattgttcgt tccgccattc gatgaaccat tgtttggatg gtttggttct
    19381 ctctgagtat ttccattgtt attcgatcca gatgggtttg tttgattcga agaattcccg
    19441 ctgttcgaat tattgttatt attgtttgaa ttatttgaac cattattatt gttgttatta
    19501 cccacatctg gtttgtttgg cgtttccgga tctggtgtac tagggacttc ttcactagga
    19561 tcagttggtt tctctgttga agtatccggt ccagctttaa attctgcagt aatagataaa
    19621 ttgttcgtac ctactgtaaa ggtctttgca tcataattta cttctttggc accattaaca
    19681 gcgactgaag caatttgttc accttcagct ggcgtaatag tgtaagttac cttcgtttcc
    19741 gctaataaac cttctaccgt tccactttcc gtaatatcca aaggttgctg ttcaggggaa
    19801 tccgctgtcg aagcagtcag ttggatctta ccaatcaaag aaggagcaat tgtaagggaa
    19861 tatgtctcgg ctggcttttc attttctgat ggatcagccg gatcaatttc agttggctcg
    19921 gtcgatcctg ttgttgagtt agttgttcca gttgggtctt ctgtaaaaga atccgttgtt
    19981 tcactagtag ctgagctgga tgtagtcgta tctgttgtga ctgtttgatc agctatttga
    20041 ttatctacgg caaatacggt attggtcgac aaaacagacg gagcaatcag ccaagtagca
    20101 gctagcaaac gtatcgtttt tttatttttc ataataaact agtccttttc ctttcattga
    20161 ttttctgtct tattcagcat tcattagaca ttttttggaa cagataatcg tgtcgaaaag
    20221 ctttttcttt tcataaatct gtctgtgtat tctaaataaa acaatttgta aataagcaac
    20281 gagcattcat tcgtttttct ttgacgttca ttttattgca tagaccacct ttgtttgggg
    20341 ataaacaagt ggggataaaa atggttatgc atatgtaaat tataccaaag tttggaaagt
    20401 atcacaaaaa tttataaaaa taatgatcct aaaaagaaaa agactgaaat atgtgataaa
    20461 catctcatac ttcagtcttt ttcattaact attcataaga cattaggtct tcatcaaacc
    20521 cgctaacgag agtagtgatc attgctacga ctagactaac agccagtcct gctaagtacc
    20581 agaaaaaagt accggaaatg aatgcaggca atgtggtcag actaccaaat acaaaagcga
    20641 tcgctttcac ttgggcaaat cccattacag ctccaccagc agctgcacca atgcaagcag
    20701 caacaaatgg ttttttcaat gggatcgcaa ttccgtaaac agttggttcg gtcactcccg
    20761 caagtaaaat cgaagcaaaa gcagaaccgg agatcgaacg ggtttttgaa cgtttacttc
    20821 gcagaaatac acctaaagct gcaccagcca ttccaaaatt ggctgctcct gtcaacggaa
    20881 cgattcggtc gaacccataa gcagagatat tactgatcat gacagggttt acagcccatt
    20941 ggataccaaa tattaccaaa atattccaac caccagcgat gattgcgcca ctgatcggtc
    21001 cgttaatatc catcatccaa gtaattgcgg cagctaaacc ttcgccaagg taaacacccg
    21061 caggcccgaa taccattaac gtcaaaggaa ccgtgataac taatgaaagt aaaggtacaa
    21121 atactaattg catattttcg cgaatatatt ttttgaggaa tttttcaaca aaagaataaa
    21181 agaaaatacc taaaatcgcc ggcatgacag tagacgtata ctgcatcagt actacaggaa
    21241 tccccagaaa atcagtagct gctttgccac tgtctgaaag taaagaggtc agattcggtt
    21301 caatcagtgc tgcagcaatg acagccgcaa tatatccatt cactttaaac gttttggcag
    21361 cagaaaaagc aagaaagatt ggcagaaaat aaaagacggc attcgacgca gcagacaaaa
    21421 tatgataagt tccgcttgtt tcactaagcc agccaatcgt agtcgctaaa acaaccagac
    21481 cttttaatat accagaaccg gcaaacagcg gaatgatcgg cgtaaagatt tccgatatca
    21541 aaccaaatag tttttcgatc ggatttagct tttctttcgg agctgaatga tcgatttgac
    21601 gcgtcaattc cttgtaagca ggagccaatc cctgatccat gattatttct gcatatctat
    21661 cgagtaaaat gaaatactta atggaaggga tttgctgttt taatttttct gcattggcta
    21721 ctggctgttt tttcaatttg atacgaagcc gagtcgcaca tggaaggaca gaatcaatat
    21781 tgtcttttcc tcccaactgt tcaatcagca tatgaatctg ctcggacagt accatctgtt
    21841 caggattcat tgtcgtttcc attagaagac cactccactc actaatataa tattggcgta
    21901 aggtgtcatt tcgcctgtac gaataaatgc atgacagtta ttcaactctt ttttcatttc
    21961 ttcatgtggg ataaagctaa tcggtgtatc tggtaacaat aatttgattt tttccaatat
    22021 ttccgggttc attgtcttta tttcttccgc caattcaatc gattgtacct caagttcttc
    22081 taaaacgttt tctaaaacag ataaaaaact tggaagaccg tttgagacag ccaaatctat
    22141 tttttcagta ccaaagggga caggcatgcc tgcatccccg atacttaatt tatcaaagtg
    22201 ccccatttgt gcgattactc gtgaaatatc tgaattgatt acttttcctt ttttcatgtt
    22261 tgtttcttcc tctcattgtt aattttagac tgctgattgt tctaaagctt cgcggtaagg
    22321 gatagaaggt tgcgcaccat aagactgcac agcaacagaa gaggctagat ttccataaag
    22381 gatcgcttct ttcaagttac taaggtctgg ctgaagtgtg ctgcttaatg cgccaataaa
    22441 tgtatcacct gcggctgttg tgtcaacggc atccacttta aaagcaggga cgataccgtg
    22501 ttctttttct gtatgataga aagcgccggc actgcctagt gtaatgatta ctgttccaat
    22561 acctaattca tggagttttt cggctgctgc gaccaaactg ttatgatctg tcactctgat
    22621 tcctgtaatg atttctgttt ctgtttcatt tggtataatg atatctgtct tttcaagtag
    22681 ttcagtagga atcgtttcac gagctggtgc aggattcagg atcgttgttt ttcctgcgtc
    22741 ttttgcaata gaaaaagcta ggatcgtcgc atctaagcta ctttcaaact gagcaataat
    22801 aaagtcactg tttgtaatca gttgcttgtt ttttaggact tcttgttcat ggaaagcatt
    22861 atttgcccct gcatggatca agatactatt ttctcctgct gcgtctacca taatcattgc
    22921 ttgtccggtt gtggcgcctt ggatttcagc tatcccagat gtatcgatgt tttcttttgt
    22981 cagtagatct aataactgtt gtccttcgct gtcagcccca acgcctccaa taaaagatgt
    23041 ccgtgcgcct gaacgtttgg ctgccactgc ttggttggca cctttacctc cgccagcgtg
    23101 aaaaatctca tgcgcatgca tcgtttcgcc tggcttgggc atgttggtta aacgaagggt
    23161 agtgtccata ttaatacttc caattactgt aaccgtgttc attttacaaa cctccaacta
    23221 tctcatttat tttgatggga aaattatttt ttaaaacgtt ttagtaaaac gttttaatag
    23281 ttcgataaaa atatagcaca ccttgaaagc tatttcaagg gtgcagttga aaatcttttt
    23341 tctaatcgta caggtaatct tttttcttcc cattcttttt ctgggaattg gatccgatct
    23401 aacaataact tggcagatgt ttgtcccaat tcgaagattg gttgtgcgat cgttgtcagt
    23461 ttcggcttga tatactcgca catatctata ttatcatagc caataatgct gtagtcttca
    23521 gggatgcttt ttccagcttc ctctaatccg cgatataatc caaaagctaa ttcatcattt
    23581 aaagcaaaca caccagttgc agcagaatca agaagttctg ctgtgatctg gtagccacct
    23641 tgttttgaga actgtgtagg taagaggatc agttggtcgt gagggatttg gtaaacatca
    23701 agagcagatt taaacccctc aatccgagcg tggacatttt ccggtggatt ttcaggatag
    23761 acaagggcga tcgtttgatg tcctagacta agcaagtgca tgcccgctag gtaaccaccg
    23821 cgaaaatcgt ctgttcgtac ggcatcacta aatccttcag attttttctg atccaaaaca
    23881 atgaagggcc ggccttgttt tttcaaattt tcgttgattg catctgtaga gacagcagaa
    23941 gtcgcgataa tgaatccatc cacacctcga cgtgtgagct cggctagata ttcgatttct
    24001 ttttgatgat cagaatcagc attacataat atcgtgacga aattctgctt atacaagata
    24061 tcctcaattc ctcgcatcag tgtactgaag aatggattcg tgatatcagg aacgagtaca
    24121 ccaattgttt tcgtttcctt ggtaatcatt tgtctagcaa aatagtcagg ctgatagcct
    24181 aattcttctt tggcagctaa aactttctga acagttttcg gactgaattt ctccgtattt
    24241 ccatttaata tcaaggagac agttgtgatt gaaacgccgg aaagtttagc gacttctttg
    24301 attgtgatct tttttttcac catgttcata ctcctatcta aatcctgtgg ttattttccc
    24361 atgaaatcaa aaagaagtaa atgaaaatca cgtgtgccag tacttatttg ctttgttgga
    24421 tagcttcgat tatttgtttc gttgagcgta ctcgtgcagt tttcggaaat atctttttta
    24481 tgaacaattg atgggattct ttgtcacgat cactgcaagc atcttcagca agcacgatat
    24541 ggtacccgtg ttgaaaggca tctaatgcag tagcatatac accgtttgtt gtcgctacac
    24601 cgcttaaaat gatagtttcg attccacgcc ggcgaagttg aagatcaaga gaagtaccaa
    24661 aaaaagcact tggattgtat tttgtgatag tcaacatatt atctgtatct ttcaaagaat
    24721 ccttcaaaag taaatcagtg aattctggag gtacagaagc agtcattcca cccatatcag
    24781 tttgttgact caaataaggg aaagtggaga tgtctacgtt cactaaaact ttcaaggcag
    24841 cggtacgttt caatgttctt actaacaaat cattgtttgt cagaatagat gctgcagtgt
    24901 gtggagagag tgtaccgtta tttacgatct ctttttgcat gtcgatcgcg acaaaagcag
    24961 tttttgataa atcaaaaatc atattttcct ttaaatcatt catgatgttc cttctttcaa
    25021 ataacaatgt tttttatgta agtaaatcat aaatcgttta tttgtcaact aaattaaagg
    25081 attcctttct ttagtctaga gaaattcagt ggtaaaataa ataagtgtga gaaaacgcat
    25141 tcgatgcata gatatagcga gaaatatcgt gaaaatggag gaatcagagt gaagtatgtc
    25201 gaagtaaaaa atgaatacaa acgatacaaa atgggagaaa cgaccattac agcaaatgac
    25261 gatatttctt tttcaattga aaaaggcgaa ttagtgatta tacttggtcc aagtggagca
    25321 ggaaagtcaa cgattcttaa tattttaggt gggatggata cacctgatga aggtcagatc
    25381 attattgatg acacagatat tgcgcaattt tcagataaac agcttactgc ttaccgacga
    25441 acagatgtag ggttcgtttt ccaattctac aatctcgttc ctaatctaac agcaaaagaa
    25501 aatgtggaat tggcaacgga agtttctcct gatgcgcttg atcctgtaga ggtattgagg
    25561 caagtgggcc tagctcatcg attgaataac tttccgtccc aattatcagg aggcgaacag
    25621 cagagggttt caattgctcg tgcactagct aaaaatccca aacttctttt atgtgatgaa
    25681 ccgacaggcg cgctggattt tgaaacgggc aagcaagtat tgaaattatt gcaaaacgct
    25741 agcagacaac acggcaatac cgttttgatc attacacata attctgcttt agcgcccatc
    25801 gctgatagag tcattcacat taatgatgct aaagtaaggt ctgttgaatt aaatgatcat
    25861 ccttcatcca ttgatgagat tgtatggtag ggggatttta tgaaaacaag aacatatctt
    25921 aaagcaggtt ttcgcgaaat tcggcaatct aaaggacgat tcattgcaat catccttatt
    25981 gtcatgctag gaacactttt atttgtaggt gtcaaaactg ctggacctgt catgcaaaaa
    26041 acgatggatc actatgttgg tacagcagga ctttctgatt tgcaaatcgt ttctactggt
    26101 ggcttgacac aaaaagatat tgctcaagca gaaaagatcc cagatgcaaa agtagaaaca
    26161 ggaaaacaga tctattattc caatactggc aaaaacgaag tgattcaaat attttcttat
    26221 aacaaaaata acaagcagaa caagctacaa ttaactgatg gaaaactccc ggaaaaacca
    26281 aaccagcttg tattggatga aaaagcggaa gatgaagggt ataaaatcgg tgatatttat
    26341 cagattgatt cagatgaatt gaaagaaaaa gagtatacca tcacgggctt tgtccgttct
    26401 ccattgttca tcaacaattt ggagagagga tacgcgaacg tcggaaatgg tagcgtcgat
    26461 tattttgtct atttacctga atccgttttt aaaacagata tttattctgt cttatatctt
    26521 gattttacaa acgtaaagaa tttgaatacg tacagcaaag cttacaaaga taaaatggag
    26581 caaaatcaag aaaaggcaga aaagtattta caagatcgac ccgaagaacg cttagaagag
    26641 ctgaaaaaat cggccaacga agaattagaa ccggcacagc aaaaaatagc ggatggcaaa
    26701 gcacaaacaa cgcaagcaag aacacggtta gaagatgcta aaaaacagtt agcgcaacag
    26761 tcagcaacta tccagcagct tcctgaagcg caacgaacaa tcgctgttca aacattatct
    26821 caacaagaag ctcaacttgc tgaacaagaa aggcaattag ttgagaaaga acgtgaactg
    26881 gcctctgcag aggaagaatt gaatcgaaat gcagaaaaaa ttgaaaattt aactaaacca
    26941 tcatacctat acaatgaacg ttcagaaaat gtcggttttc aagaatttgg tgatctgtct
    27001 gatcgtatcg ctgcgattgc caatgttttt cctgttttct ttttctttat tgccgcattg
    27061 atcaccttta cgacaatgac acgtatggta gaagaaaacc gcagggaaat cgggacatta
    27121 aaggcgttag gttatacgaa agttgaaatc gcaaaaaaat atgcaatcta tgcaagtcta
    27181 gcctcaggga taggtattgt tttaggaacg atattaggaa cgaatctttt gccaaggatc
    27241 atttttgagc tgtctaacga gcggtatgat attgggagtg cgcttatttt ttatgactgg
    27301 ccgccgattt tacaagcagc tgccgcattc ttcattgccg catttggcgc tgcaatgatc
    27361 gttttattca aagatttaag agaacggcct gctgcattgc ttcaaccgaa agcaccgaag
    27421 ccagggaaac gcatttttct tgaatacatc actccgctgt ggtcacgcct tagctttaat
    27481 caaaaaatca gttaccgaaa tctcttccga tataagtcaa gaatgcttat ggcgattatc
    27541 ggaattgctg gatgtgccgg gctgatggtt gcaggcgtgg gattgaaaga ttctctaagt
    27601 tctgtttcag ccaaacaatt tggaccgatc attgactatc aagcaatcgt tacattggat
    27661 tcatccacgg aagatagtcg tcaaaaagca gcggatatgc tgcagaagca agaaaaagta
    27721 aatgagatcc tagctattca tactcaaacc gtagaattga gaaaaaaagg acatgcttcg
    27781 caaagtctga cagtgatcgt accaagtaaa acagctgatt tggatcgttt tatccattta
    27841 actgatcttg aagagaaccc acgctccttg cctaaaacag gaatcgctat tacgcaaaaa
    27901 gtagcagaac tatttgactt atctacagga gatcatattt cgctttatga tgaagatcaa
    27961 aaagaatgga aagtcgaagt aaaagaaatc gtgaaaaact atctaggtag ctttatttat
    28021 atgtcccctg aatattatgc ttcagttact gatcaatcga tgatggataa tgcatttttg
    28081 ctgcgtacag ataagatgac aacaaaagaa gaagaaatgc tttccgagaa actgcttgcc
    28141 acagacgctg tgacaaatac gtctttcatg tccaaacaaa tcgaaacgca agaagaatca
    28201 atggcgaact tagactcggt tgtcttaatc tttgtcgtat tatcaggact gctggctttt
    28261 atcgtcttgt ataatctaac taatatcaat atttccgagc gtgtacgcga attatctaca
    28321 ataaaagtcc ttggattttt tgataaagaa gtcacgatgt acattgttcg agaaaatatc
    28381 atctttactc ttttaggaat catcggagga tttggtgtag gatatatatt gactgacttc
    28441 atcttgcaac aagcgtctat ggagaatgtc atctttccat tagtgatcac atggtgggct
    28501 tatgtcttat cagcaggatt aactatcgta tttacggtta ttgtgatgat tgttacccat
    28561 ttcaaattaa agcatatcga tatgattgat gcgttgaaat cgaatgaata aaaacagagg
    28621 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggcaaaat agtttttcat
    28681 attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccat gaatcagagg
    28741 ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc aaataaaatt agctcctcat
    28801 gttttacatt tgagaagact gattgccgta gataaaagct tttgaacacc atttatagaa
    28861 gaaacaagaa tattttgcta ttttaaacaa gaggatagac aaatacatgt ctgatctctt
    28921 gttttttatt gggcaacaga cctcttagaa tgtataaaga taaattttac aaacgctttc
    28981 ggttcagtta taataataaa tgataacttg tatatataag ttgtggtagg taataaatca
    29041 gttgaatgga ggtaaatatc catgacattt aaagacaaag tcatttacca aatctatccc
    29101 aaatcttaca aagacagtaa tggtgatgga attggagatt taaaaggaat caaagaaaaa
    29161 ctcccgtatc tgaatgatct aggcatcgac atgatttggc taaaccctat ttatccaagc
    29221 cctcaaaaag acaacggtta tgatatctct gattacacag ccatcgatcc tatctttggt
    29281 acgatggagg aattcgaaca gctaatagcc gaagcaaaga aataccggat agaaatcatg
    29341 ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat ggttcaaaaa agcactagca
    29401 ggggaccagc attatcagga cttcttcatt ttaagagaag agccaacgga ttgggtatca
    29461 aaatttggtg gaagtgcttg gtcgccattt ggagatacgg ggaaatatta tttgcactta
    29521 tatgatcgga cacaagctga cttgaactgg cggaaccaag cagtaagaga ggaactattc
    29581 aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat
    29641 gttatcggta aagacgaaca attgaaaaat aataaagaaa atgatggcaa acctgaatat
    29701 acggacaaac caatcaccca tacgtatcta aaagaattga accaagcaac gtttgggacg
    29761 gattccacta tcatcacagt cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg
    29821 tatacagaac ctagtagaga agaattatca atggtcttta atttccatca tttgaaagtc
    29881 gattatgaaa atgggaataa atggtctact cctccatttg attttgcaga attaaagcga
    29941 ctatttcata catggggaga aaagatgagt gagggtaacg gctggaatgc tctatttttt
    30001 aataatcatg atcagccacg tgcgctcaat cgatttgtag ctatcgatca gtatcgaaaa
    30061 caaggagcag agatgctagc aaccatgatc catttgaacc gtgggacgcc ctatatttat
    30121 atgggagaag aaatcggcat gatcgaccca gactattcat cgatttccga ctatattgac
    30181 gtagaaagtt tgaatgctta taagttactc cttgaacaag gattcacaga aaaagaagcg
    30241 tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa cacccatgca atggtcagac
    30301 acagaacaag caggatttac tacagggaaa ccttggttaa aagttggagg caagctagaa
    30361 gagattaatg tagaaaaaga actatcttct gaagattcta ttttgtccta ttacaagaaa
    30421 ttgattcggc tgagaaaaac atatccaatc gttgcacaag gagattatca tgcttatgaa
    30481 gcaaatcatc cggaagttta tggctttctt cgccaatttg aaggacagca gctgcttgta
    30541 ttaacaaatt tctatgataa agatacaagg atcgccatcc cagaaggatt cacagaagcc
    30601 aatattctta tcgataatta ttccgatact gttcttcatc aaagtctgga gctaaaacca
    30661 taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag
    30721 ctattctcgt aagatcgacg ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa
    30781 gagttttcat actctccttt atcgccataa aatttattgt agcgttgttt gaatttacgg
    30841 aaaataaaag taacgagtaa tttgactaac gtatagatag gaataccaaa aataacgccc
    30901 acaattccca ttagatcacc catgacaagt aaaacgatta agatcgtaat cggatggatc
    30961 tgtaaacgat cacccattac tcgaggaatc accaagtcac cgtggagaag ttgtacggca
    31021 aaccagacaa tgacaaattt gacagccata aaagtagagt cttggaatgc aatgatcaaa
    31081 cctggaaaga aggcgataaa cggcccaata tatggaatga tacataacaa cccagcagaa
    31141 atagctaaaa cactagcaaa ttctaatccg atcagtaaga aacaaaccca gtagactgct
    31201 ccaaggataa atgaagcaat gatttgcccc tttagaaatg aacccaattg ttgattagca
    31261 atctttgtca aatttttaaa atctgtacga aatgctggtg ggacaatcgc taaaactgac
    31321 tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga caattggacc agtaaataaa
    31381 gtaataaaaa tagctgtaac agttgaaaaa atgttgctga tccgttgtgc gccactttcc
    31441 caataatttc caataaatcc agctatatca gacgtcacgt cgtcaataga agcgaaaaat
    31501 tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa
    31561 tcttgaaaca aatctggcaa actactgatc aatgtctttg cctgttcttc aagaaaagga
    31621 ataagccata tgcttccaag ccccaataaa aataaaataa tcacataaac gataatgact
    31681 gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga ctggttccaa aagataatac
    31741 aagatcagtg caaacaataa tgggggcaag atagtagcga taatgattcc gacaggcttg
    31801 aatataaacg agatctgatg gaaaaaataa atgatcagcg caagtaagat aactattcct
    31861 aaaacatagt aagaggtttt tcctcctaaa aagcggatca aacgattcgt tgtatctgtt
    31921 tcttttttca ctctttttcc tcctttatct ataacttcat tatagacaaa agcgggtata
    31981 aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa tcattcatat aaactttctc
    32041 atcaattaaa ataagaaaac gcttgacagt tcttttaacc atatgataaa ttaaatcctg
    32101 ttgctaaaac gtaactatta cggtttgcga aaagcgaatc ggaacattca aatgaaagaa
    32161 gatgagaaga ggagtgtcat aaatgaaacg attgataggt atattggcca tgctggtttt
    32221 agcaggtatg ttaactgctt gcggggcaag tgggaaagcc gaggacagta aagaaaaatt
    32281 gagcgtgatg acgacctttt atcccatgta tgattttaca aaagcaatcg tcggggatga
    32341 aggagaagta gagttattga ttccagcagg gactgattcc cacgattatg aaccttctgc
    32401 taaagacatg gcaaagatcc aagatacaga tatctttgtt tataatgatg aaaacatgga
    32461 aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc aatgtccata cgataaaagc
    32521 gacagaaggt atgcttttac ttccgggaag tgaagaagga catgatcatg accatgaaca
    32581 cggggaagaa ggccatacac atgagctaga cccacatgta tggctagctc caagtttagc
    32641 aatcaagcag gtagcaaaca tacgtgatca gctgattgaa gcttacccag aaaaacagga
    32701 agtatggaca aaaaatgcag cagcctatac cgagaaactt caagcactcc atcagcttta
    32761 tcaagaaacc tttaagcaag caaaacaaag atcttttgtc acgcaacatg ctgcattcaa
    32821 ctatctagcg ttagaatatg gattgaatca agtatcgatt gctggtcttt cttctagtga
    32881 agaaccgtct gcggcgagaa ttgctgaact gaaacacttt gttaaagaac atggaatcaa
    32941 ctatatttat tttgaagaaa acgccaaaga tagtattgcc agaacgttag caaacgaagc
    33001 aggtgtatct ttagaagtat tgaatccttt ggaaggattg acgaatgaac aaatagaaaa
    33061 tggagaaaac tatctttcta tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga
    33121 gacagaaaac ccgctagaag atactttgac ccccaaaaaa gaaaagagcg tctacaacgg
    33181 ttattttgaa gacgaggatg tacaagatcg ctctctttca gattgggcag gaacttggca
    33241 aacagtagat acgtatgtag aagatgggac ttttgatcct gtttttgaat ataaagaaaa
    33301 attgaatcag gataagacag cacaagaata taaagaatat tatacgaagg gctatcaaac
    33361 agatattgct tcaatcaaga tcagtgagaa agagatggca tttacgttta agaatggaac
    33421 aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa
    33481 ccgtggcgta aggtatctat tcgaagcaga agataaggaa agtggcgctt ttagatatat
    33541 tcaattcagt gatcacacga ttgcaccggc tgactccgaa cattttcatt tatatgcagg
    33601 aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga
    33661 tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa
    33721 ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga
    33781 aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac
    33841 ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg
    33901 acaacggata aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa
    33961 ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata
    34021 ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc
    34081 tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat gctcgacatt
    34141 ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt
    34201 taagattttc atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc
    34261 taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat
    34321 atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc
    34381 cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt
    34441 gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat
    34501 catatcatta ctcctttatt ttatttgtat acccgtggtg ctagt
//
LOCUS       DACIRY010000018        34432 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.018, whole genome shotgun sequence.
ACCESSION   DACIRY010000018 DACIRY010000000
VERSION     DACIRY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.018"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..748)
                     /locus_tag="IYE22_000715"
     CDS             complement(<1..748)
                     /locus_tag="IYE22_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9505948.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATI"
     gene            complement(972..2912)
                     /gene="thrS"
                     /locus_tag="IYE22_000716"
     CDS             complement(972..2912)
                     /gene="thrS"
                     /locus_tag="IYE22_000716"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ9505949.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3249..3428)
                     /locus_tag="IYE22_000717"
     CDS             complement(3249..3428)
                     /locus_tag="IYE22_000717"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ9505950.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3475..5298)
                     /gene="cadA"
                     /locus_tag="IYE22_000718"
     CDS             complement(3475..5298)
                     /gene="cadA"
                     /locus_tag="IYE22_000718"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ9505951.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5576..7855
                     /locus_tag="IYE22_000719"
     CDS             5576..7855
                     /locus_tag="IYE22_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9505952.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(7948..8595)
                     /locus_tag="IYE22_000720"
     CDS             complement(7948..8595)
                     /locus_tag="IYE22_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505953.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8774..9493)
                     /locus_tag="IYE22_000721"
     CDS             complement(8774..9493)
                     /locus_tag="IYE22_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ9505954.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9624..10775)
                     /locus_tag="IYE22_000722"
     CDS             complement(9624..10775)
                     /locus_tag="IYE22_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ9505955.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(10992..11732)
                     /locus_tag="IYE22_000723"
     CDS             complement(10992..11732)
                     /locus_tag="IYE22_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9505956.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11736..12059)
                     /gene="rsfS"
                     /locus_tag="IYE22_000724"
     CDS             complement(11736..12059)
                     /gene="rsfS"
                     /locus_tag="IYE22_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ9505957.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12116..12709)
                     /locus_tag="IYE22_000725"
     CDS             complement(12116..12709)
                     /locus_tag="IYE22_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9505958.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12693..13340)
                     /locus_tag="IYE22_000726"
     CDS             complement(12693..13340)
                     /locus_tag="IYE22_000726"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ9505959.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13353..13664)
                     /gene="yhbY"
                     /locus_tag="IYE22_000727"
     CDS             complement(13353..13664)
                     /gene="yhbY"
                     /locus_tag="IYE22_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ9505960.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13680..14789)
                     /gene="yqeH"
                     /locus_tag="IYE22_000728"
     CDS             complement(13680..14789)
                     /gene="yqeH"
                     /locus_tag="IYE22_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ9505961.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14786..15313)
                     /locus_tag="IYE22_000729"
     CDS             complement(14786..15313)
                     /locus_tag="IYE22_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ9505962.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15493..16278)
                     /locus_tag="IYE22_000730"
     CDS             complement(15493..16278)
                     /locus_tag="IYE22_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ9505963.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16271..17140)
                     /locus_tag="IYE22_000731"
     CDS             complement(16271..17140)
                     /locus_tag="IYE22_000731"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ9505964.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17141..18520)
                     /locus_tag="IYE22_000732"
     CDS             complement(17141..18520)
                     /locus_tag="IYE22_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ9505965.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18528..18947)
                     /gene="fabZ"
                     /locus_tag="IYE22_000733"
     CDS             complement(18528..18947)
                     /gene="fabZ"
                     /locus_tag="IYE22_000733"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ9505966.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(18972..19448)
                     /gene="accB"
                     /locus_tag="IYE22_000734"
     CDS             complement(18972..19448)
                     /gene="accB"
                     /locus_tag="IYE22_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ9505967.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19451..20686)
                     /gene="fabF"
                     /locus_tag="IYE22_000735"
     CDS             complement(19451..20686)
                     /gene="fabF"
                     /locus_tag="IYE22_000735"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ9505968.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20705..21442)
                     /gene="fabG"
                     /locus_tag="IYE22_000736"
     CDS             complement(20705..21442)
                     /gene="fabG"
                     /locus_tag="IYE22_000736"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ9505969.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21454..22371)
                     /gene="fabD"
                     /locus_tag="IYE22_000737"
     CDS             complement(21454..22371)
                     /gene="fabD"
                     /locus_tag="IYE22_000737"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ9505970.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22478..22702)
                     /locus_tag="IYE22_000738"
     CDS             complement(22478..22702)
                     /locus_tag="IYE22_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ9505971.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22755..23717)
                     /locus_tag="IYE22_000739"
     CDS             complement(22755..23717)
                     /locus_tag="IYE22_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ9505972.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23727..24167)
                     /locus_tag="IYE22_000740"
     CDS             complement(23727..24167)
                     /locus_tag="IYE22_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9505973.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24473..25438
                     /locus_tag="IYE22_000741"
     CDS             24473..25438
                     /locus_tag="IYE22_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ9505974.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25495..25725)
                     /locus_tag="IYE22_000742"
     CDS             complement(25495..25725)
                     /locus_tag="IYE22_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ9505975.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25811..26698)
                     /locus_tag="IYE22_000743"
     CDS             complement(25811..26698)
                     /locus_tag="IYE22_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ9505976.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26787..28010)
                     /locus_tag="IYE22_000744"
     CDS             complement(26787..28010)
                     /locus_tag="IYE22_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ9505977.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28027..29106)
                     /locus_tag="IYE22_000745"
     CDS             complement(28027..29106)
                     /locus_tag="IYE22_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ9505978.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29237..30964)
                     /gene="ptsP"
                     /locus_tag="IYE22_000746"
     CDS             complement(29237..30964)
                     /gene="ptsP"
                     /locus_tag="IYE22_000746"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ9505979.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(30964..31230)
                     /locus_tag="IYE22_000747"
     CDS             complement(30964..31230)
                     /locus_tag="IYE22_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ9505980.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31709..33943
                     /locus_tag="IYE22_000748"
     CDS             31709..33943
                     /locus_tag="IYE22_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ9505981.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34091..34411
                     /locus_tag="IYE22_000749"
     CDS             34091..34411
                     /locus_tag="IYE22_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ9505982.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9716 a         7464 c         6014 g        11238 t
ORIGIN      
        1 cgatcgtagc aaattgggcc aaaagtgaat tagttgccgc acttcgattc gtgatttctt
       61 ttggtctaga tgtttgtacg agtacatcgg ctatcttgct aagcgggtta tttccaaatt
      121 gcgtcaacgc aatgatttcg atattcatgc ttttcgccag ctcggctaat gcgactacat
      181 ctgcacttct acctgagttt gaaacaagcc aaagcacttt ctttttctca ttgctgacaa
      241 gttgcgggag caagacgtgg atatcttttt caaaaatgat tggcttaccg acacgagacc
      301 atttttgcag aatatcttct actactaaag aagaggcgcc aaccccatat aaaaaaacag
      361 tctctgccga ttccaaacac tctacgactt tttggatcgt ttcttcttct agaatatcta
      421 ctgtttcttt tatcgctact tgtgcattgt taccaagttt gtttttgata gagtaaaagg
      481 actcatttgc ttctatatca ctaaaacctt catcgattcc agtctgtagt tctgttgaaa
      541 tcgtgatttt gaagtccgtc agactttgaa aaccgatttt tttggaaaaa cgtacaactg
      601 tcggcgcact caacccggaa gcttctgcta attcattagc tgtcattgta gggaccttct
      661 caatattacc gataatgtag ttggccaact ttttttctgc tgaagaataa tcttccatca
      721 tactttcaat acgactaatt acgctcataa gttcctccac ccttttaatg atagtctatg
      781 taagtatacc atatcagacc cttattcacg gtcgtatact ggataaaaaa caaaaaaaca
      841 atccgcccaa cagaatatct tatcccatca atcccacaga ttctattgag catctgtgtc
      901 tttgaggaat acctgttatc tattgttcgg attgctttct atctactact tttttacagg
      961 aaatttacct attagttttc tctgctgtaa tttttgactt ctgcttccat agcatcaata
     1021 aagatattca aatcttctac agctgtttct ttacttccat aacgacgtac gttcactgtt
     1081 gcatcttcca attccttgtc tccaacaact aattggtaag ggatcttttg tgtttgagag
     1141 gcacgtatct tatagcccat tttttcatta cgatcatcaa cttctacacg caaccccttc
     1201 atttgcaaac gttctttgat ttcataagca taatcgccat gtgcatcaac agaaacaggg
     1261 atgattgttg cttgtatagg tgccaaccat gttggaaaag cgcctttgta tacttctgtt
     1321 aaataagcga caaaacgttc cattgtagaa acaattccgc ggtggataac tactggacga
     1381 tgcgtatttt ccccgtcttc tccaacatat gtcaaatcaa agcgctctgg caataagaag
     1441 tctaattgta tagttgataa tgtttcttct gtaccaagag ctgttttgac ttgaacatct
     1501 agttttggtc cgtaaaaggc tgcctcgcct tctgcttcaa agtaatctag ttctaattca
     1561 tccatagcgg ctttcagcat tgcttgggct ttttcccaca ttgcgtcatc atcaaaatat
     1621 ttatctgtat tgtttggatc gcgatagctc aagcggaaac gataatctgt aatattgaag
     1681 tcagcatata ctgccaccat caattcaagc gtacgtttaa attcatcttt gatttgatca
     1741 ggacgaacaa atgtatgacc atcattcaac gtcatttctc gtacacgttg taaacctgac
     1801 aaagcacctg atttttcata gcgatgcatc attcctaatt cagcgatacg aattggtaat
     1861 tcacgataag aatggatcgt gtttttgtag accatcatgt gatgagggca gttcattggg
     1921 cgaagaacaa gcatttcgcc atctcccata tccattggcg ggaacatgtc ttcttgataa
     1981 tgatcccagt gtccagatgt tttgtacaac tcaacatctc ccattattgg tgtatataca
     2041 tgttggtaac ctagactaac ttctttgtct acgatataac gttcgatcgt gcgtcggatt
     2101 gttgcccctt ttggaagcca gaatggcaaa ccagaaccta cttctgggga aaccatgaac
     2161 aagtcaagtt cttttcctag tttacgatgg tctctttctt ttgcttcttc acggagacgg
     2221 atatattctt tcaaagcttt cttgtcaaaa aaggctgtcc cgtatacacg ttgcatcatt
     2281 tgattgtttg agtttccacg ccaataagca cctgccactg ataataattt aaatacttgt
     2341 atacggccag tggatggaac atgtggtcca cgacacaaat cgatgaattc gccttgttga
     2401 taagcagtaa tcacttcttc ttctggcaat tcactaatca gttctacttt gtacggatca
     2461 cttgcaaaaa tttctaatgc ttcctgtttt gaaagaacac ggcgttcgat cggcagattt
     2521 tctttgacga ttttcatcat ttctgcttcg attgccggca aatcttctgc agttacttgg
     2581 ttttgtccat tatctgtatc gtagtaaaac cctgaatcga tagctggtcc aacgccaaag
     2641 tggatatttg gatagaggcg acgcatggct tgggccatca aatgagctgc tgaatgacgg
     2701 actaagccaa gtgcatcttc atggtctggt gtgatgatct caatacttgc atcttcctcg
     2761 attggacgag tcaaatcaac gagttttccg ttcactttac cagctaaagc tttttttgct
     2821 aagctttttg aaatgctttc agcaattgcc aatgttgttg ttccagactc aaattctttg
     2881 actgcaccat ctggaaaagt aatttttatc ataatagttt cctccttgaa atgttttgat
     2941 tgatcagatc atcaagctga aaaacaaaaa aatcctagca tctcttgaaa agagatacta
     3001 ggacgaaaag ttcgtggttc cacctaatgt tcaaacgcaa atgcgttcct caagcgttgt
     3061 aacggtccga ccgcccttgc ttcaacaagg gtttcgaaag tggtcatcct ttggttttct
     3121 cctggaaaag tttcagccta gcttttcctc tcttatcgaa gactctcaaa ttcagttggc
     3181 tttctcatca atcaattatg tgtttattta accactaata aatagaaaaa tcaagtggga
     3241 aactaaactt attttttcca ttccccattt acaccgtatg ttcctttttt catctcattg
     3301 atgatgacat ggatgtgttc ttttggtgcc tgcgtgtttt tatgaacagc ttccgtgata
     3361 tctttcatca tattttctaa ttgttccgga ctgcgacctt ctactaattc gacatgtacg
     3421 aatggcataa tgctctctcc tctttctgat cgttcttttt tcaactcgat tctatcatac
     3481 tatgtttgtc ttgggtactg aatcaggaaa aatggctgga tctttttttg ctctcaaggc
     3541 actcaaaata gagaccgtat ccacgacttc ttgcaaaaca gctccgaata atgcggggat
     3601 tagtcccgta cttgcaatta acatcaaaaa aatacaaata aagatgccaa tcagtacaga
     3661 ctgtttcgct attctcatcg tatctcttga aatcactact gcttttgcta cacgcaagag
     3721 atcatctttc aagatgaccg catctgcact ttcactagca gctgtagaac catgagcccc
     3781 cattgcaata ccgatatctg cagcagcaag tgctggtgcg tcattcacgc cgtctcctac
     3841 catcgcaaca ggtctttgat ctttagggat actttctaaa atggtcagtt tatcttgagg
     3901 caagctacgg gcatgtacag aatctaattg gactttttct gcaatcgttt gagcaatggc
     3961 agatccatcc ccggtgatca tcatcaaatt tttcacatca agttttctta gctccttgat
     4021 tgtctttgct gcttctgggc gaatctgatc cgaaaatagg atcgttcctg caaaagatcg
     4081 atcaattgtt acatagattg ctgtattgga cgtttctatt tcttcagctg aaacaaaaga
     4141 ggcacgtcca atctttactt catgattatt gactactgct tggattcctt gaccggtcac
     4201 ttctttcagt tgactaacag gatacagttg gctgtttttc acgttgttca ctaacgaacg
     4261 agctaagata tggctagatt cctgttctgc actagctgca tacagaagta gctcttcttt
     4321 ttttattcca ttgactggct gaatcgcctc tacttccaat atccctttgg taagcgttcc
     4381 tgttttatca aaagcaatcg tttttgtacg tgcgagcttt tcaatcgttg ttccagtttt
     4441 tacaacgatc ccgtttttac ttgaacggct cattcctgca actaaagcaa tgggcgcagc
     4501 tagaatcagt ggacaaggtg atgccactac cagtacctca gcaaatcgta aagggtcttt
     4561 tgagaaccac caagaaatac caccgatcaa ataagcaatc aacgtgaaag ggactgcata
     4621 acgatcagct aaacggacaa atcgggcagg cttttctttt gattcttcga ctagtttaat
     4681 aatcatttga tactggctgt ctgcggcacg tttttcagcg atcatttgga tcgatccgtc
     4741 tccatttaaa gaccctgaca taatctgatc tccaggtttc ttggtttccg ggcgggactc
     4801 acctgtcaaa gaagattcat cgaccgaaga ttctcctttg gtcactcgac catctgcggg
     4861 tacaagttcg ccaggcttga ctagtaactg gtcatcaata tttatttcat cgattactat
     4921 atcctgcaat tttccttctt tgaatcgatg ggcaattctt ggggtgttat ctaacaaaga
     4981 ctgcagttct ttacccgctc gtttattcgc gtaatcctct agactatctc cgcccgtaag
     5041 catgatcaat atcatcaaac tagcccagta ttctttgacg gctaatgttg caaggattgc
     5101 ggtaatcgct aatacatcta cgccatatct tcctgaacgt aacgtcttga tcatttccaa
     5161 aaacatcaag aaggcgagaa tccctccagt aaatgcgaca atcgcaaatg caagggccgg
     5221 ctggtgaaaa acaaattcac ttaataaagc tatcccacca ataagcaaag taagtaaaaa
     5281 ttttttgaaa tgtgtcattg ttttttctcc tcttttatgt gacttagaga ggttagtaat
     5341 aaatttccag ctgcctcttt catcttaagc atagtccttg actagcactt tttaaaacaa
     5401 taggccttaa ttgaaaacaa ctatcactat cagcgtttcc aaaattcatc agttctacaa
     5461 acaaaaatat tcagcaagga ttactttttc gattcatcag ttaaaaagta ccttttttcg
     5521 aaaaactatg ctatactttt aattcgttat ttacggcgga aggagcaaac atgcgatgaa
     5581 tgagaaacaa ctagaacaaa cgcacgtgga tgaaacgatc gaactaatcc agatcgaaca
     5641 aaatatgctg gatacaaaac aacaagaatt gactcaggaa atgaatcatt cgacaaaaga
     5701 cacagctaat cacaagattc gagctggttc caatgaagct ttttatgaat ctgctgttga
     5761 atatcgccaa catgagcagg aattgctttt gaaataccat actcttgaag cacagcaaaa
     5821 acgcctgcaa acgttgtctg tcatgaaaga tagtccttat tttgcccgta tcgactttaa
     5881 agaagaccgt gaaacagaaa cattatatct aggtatcgct tctttaagag atctggcaga
     5941 agaaacaatc gttattgact ggcgcgcacc aattgccaat ctttattatg aaggtgagat
     6001 tggtcccgca ttttatgaaa ctgaaacaga tagaatagat gtcgatctgc agttgaaacg
     6061 acaatttaaa atccaagaag gacgtatttt atctatggta gatacgtcgg aagtaatcaa
     6121 tgatgacttt ttgctagaga tattggatga atcatcaagc aaccatatga aaaacatcgt
     6181 ctctacgata caaaaagcac aaaatgcaat cattcgggac acaacaagta aagtaatgct
     6241 gattgaagga atcgccggaa gtggaaagac atccgctttg cttcaacgta ttgcttttct
     6301 gttgtatcat aatcgcaagt ggctcgaggc tgaaaacgtt cttctatttt cacccaacca
     6361 tttgttttct gattacatct cgactgtatt gccttcatta ggtgaaagcg gcgtgcctac
     6421 acagacattc aaaaattatt tggtgcagct gctgccagag ttttctttac tagatgaaca
     6481 gcaacaagaa agtggttttt tgctagggga aaaagatccg atacaagtga tgaaaagcgg
     6541 attgacctta gtcgatcaga ttgatcgcta tatccaaggc atcacaaact acggtccctt
     6601 gttccgtgat atgaaaataa atggccgtac gatcctatca aaggaatcga ttcgccaatg
     6661 gtataaagaa acaaatgaac aattaccttt acatcataga ctttctctat tacaaacgaa
     6721 gctcctgaaa aaattaggcg gtctgcaaaa agacgaaaca cgtcagcaat gggtcaaaga
     6781 tctggcggaa gaacagctcc aagaactata cgcaacagat ccaaatcttg aatatacaga
     6841 acaaaaggag cgttcattac gtaaaaaact ggccaaccaa atcgtacgta aaagattccg
     6901 tcgaatccat cgagggatca tgaattatca atttgtcaat ataacgaaac aatatctcca
     6961 ctttctacaa actgtgccac tttctcttct aacacgacat aaaatcagcg agaccaggtg
     7021 gcaggaacat ttgacaactg ttcgtacaca cttgcgtcaa agagaattgc gtcaagagga
     7081 tgctgtacta ttctttttac tgatgcgccg aacatatcca gtgacggtta cacacaaagc
     7141 tcgctatatc tttattgatg aaatgcagga tttcgcacca tcacaagtgg cattgctgca
     7201 agccttgttc ccaaaagcaa atttgacttt ctgtggagat ctaaatcaaa atgtctttgg
     7261 aaatgaaacg atcgttggat cattggaaga ccttttcgtg gaccgtgaag tcactcgttt
     7321 ccaattaacg acaagctatc gctccacaaa agaaatcact gattttgcta atcattttat
     7381 tgtgaaagaa catcaagtag aaacaacagc aagaaaagga gaccttcctt tgctcatcca
     7441 aggaacaaca gatgatatca aattgaattg gttagcagat accattcgaa cgactcaggg
     7501 aaaagcccgg tattggcgta cagcaatcat agctaaaaca caagaagaat gcgaagctct
     7561 ttatgctcaa ctgccagacg agttgaaaca acaagtccag ctgattttaa atgagagtga
     7621 ctttatgaag cgttccatta ttttacttcc agccttttta gcaaaaggtc tggagtttga
     7681 ccgagttttc ttgtgggatg tagatgcaga acatttttct tctcagcaag acaaattgat
     7741 cctttacacc atgtgtacac gtgcgatgca tgaattgacc ctttttacct ccaataatga
     7801 aagtcctttg ttaaacaata tggatatttc atcttatgag aaaaaagtac tgtaagtaaa
     7861 aagacaactg ctatcattaa tgttagcagt tgtcttttta tttacaataa taattgcaga
     7921 aaaagtaatt caatcattta aggtcattta ttctatatat aaatccaaat ctgtttcagg
     7981 atattttaat ttgacaagcg aaataatcat tttcagttct tccatgtttt ctggatattc
     8041 tgggtcaaat accttgctat gacggccaat cccgccattg tcgatcaatc ggtaaattcg
     8101 attccattca atgaattggt caattgattg gaaattgctg attggatagc cgccacaatc
     8161 caaaatctct gatacacgaa atgctaggta tgcactatcg atcactttat tttttttcgt
     8221 gtctatcttc tcatttcttt cgataagaat tttttcagga cgatgtttca agctccactc
     8281 taatagtaga aagaaagcct ccatccgata ctcttcttca aataaccgaa aggctgaatc
     8341 agaacgacga aaaagcagcg gaatcagata attttgtcta taaatcagca ttggtgcgcg
     8401 gtccaccata cactctaaat aacggttcag ataccctgta ccgacatagt ttcctaatcg
     8461 atagccttca acgccgaatc ttgcgtattt ttgcgctaat tttgaacaat cagttacgtt
     8521 cacagctaac tcttctactt tgcggtaacc gcttaagata cgttcaaaag gttctaaagt
     8581 gatttttgct tccatattct tccccgtcca tcttccacat acttcatatc atttcacatg
     8641 atacttatgc tctcatcata ccacaggttg aattagaaaa gaaaataaat cacacaaaaa
     8701 agcaagacaa tctgttagtg agattgtctt gcttttcgaa aaagactgaa tacttttcgt
     8761 taaaactggt tgcttagtct tctaatgcca cattatggaa cacatgctgt acatcttcta
     8821 gatcttccag tgcatcaatc attttttcaa atgtttcttg atcttctgga gatagtgtta
     8881 cctcgttttg agggatcatt tggatctcag cgattgaaaa atcagtgatt cctttttctt
     8941 ttaatgcttc ttgtatacta tggaaatccg ctacttcacc gtaaacgatg atttgtcctt
     9001 cttcttccat tacatcgcga acatcgatgt ctttttccat taggtattca aggatctcgt
     9061 cagcgtcttc tcctgcaaaa gcaaagattg ctgtattgtc aaacatgtaa gaaaccgctc
     9121 cggaaacacc catgttcccg ccatttttcc caaatgctgc ccgtacgtct gctgctgttc
     9181 gattcacatt gttcgtcaat gtatcaacaa tcaccattga gccgcttggt ccaaatcctt
     9241 catagcgcaa ttcagagtat gtttcatctc ctgcaccttt tgctttttcg attgctcggt
     9301 caataatatg ttttggtaca ttataagttt tagcgcgatc aatgacgaaa cgtagttttt
     9361 ggtttgcatg cggatcagga tcacctgatt ttgctgccgc gtagatttca atcccaaatt
     9421 ttgcataaat ccggctatta ttagcatctt tagcagtttt ctttgctacg atatttgccc
     9481 atttacgtcc catattttca cttcgctttc tgatttgata gtttcataac gcaaaaacgt
     9541 cctgttccat tatacttgtg aatcacgatt ctgtgaagct ttttttcttt cttccttcat
     9601 tcttctcatt tttagtcaat tatctatact cggattggga tttgcccgaa attctgttcc
     9661 ggaatattgc ctcccaattg atagatttga tcacttctaa ctaacagttc cgcttcttgt
     9721 gcctcttttt ttccgattcg ggaataaata gaaatctctt ttttttcttt taagaatctt
     9781 cttccagcat cggtaaatcc aagtagtcga agataatttt tattttgttg gacaggaatc
     9841 tcttgctttt tgatattcag caagacataa caagataatc gttggatccg tgtccatgta
     9901 tatctttttg tttttacctt tgacacaaaa tcctcaaaac tatcagcagt tgcagctgct
     9961 gttttcatgc gcgattctat tccttcggtc atctgataga tatctgtcag ttccagcaaa
    10021 gtagaagata acaaccgata acgcaaatac ggccaataag attcccaagt ctgcagcgaa
    10081 caagttgaaa gatcttcttc tgtctgaggc ggcacaagtc cagctattct ttttccttct
    10141 tctaacgcat ggcgaatggc tgttgcactg gctatcctct gatctgtgat gattttttca
    10201 tgatagcctg cacttttacg tcggatcgga tgtaattcca tagggtggac atacttggca
    10261 ttttcctttg cataacttaa tcccaagata tgatttggcg aagaaaaatc aagcgtaata
    10321 tcgggataca gactgcgaaa aacctcagtc attttttgcg gataactata cgtttgatca
    10381 gacagttctt gaaatgcctc ttctatccta tcttgattct cttggacaaa gcgtccgaaa
    10441 ctgctgtaat caaacgaagt ctcttcttct gttccaaaac acaaactgtc acagtccagt
    10501 gtttgcaaga tttttactgc acctttagca aaatagtcag ctgactgaac cgcccattcg
    10561 acgggaagct ccactactaa atccacacca ttttgtaaag cttcattcgc gcgatgccat
    10621 ttatcgatga tagccggttc gcctctttgc aaaaaattcc cgctcattac agcaatgacg
    10681 acctctgctc ctgttatttt tcgtgccatt tccacatgat agcgatgtcc attgtgaaaa
    10741 ggattgtact ctacgataat cccgcagctt ttcatgcgca tcctccttca tagttatcga
    10801 taaaaaagaa atagagacaa gtaaaaaaag gcaaaaatcg gaaaaaatac ccgaactata
    10861 taaactattc tcgcaaacac aatacttctg cgacgaaaag ctgcactgca agagcaatgc
    10921 ctatatcgtt cggatatata atttatgttg aagatttatt cttttcttgt taactcgagc
    10981 aggcaaaacc gctagtcttt gtatgctaca aagaaccagc gctttgattt ctctgatgct
    11041 gcttcgtctg taaaatcaga aaatgcttcg actttcgaaa agccgctgtt ttccagcatt
    11101 cttaaataac tttccaatgg ataggtacgc tcttgatgta gttcatcttc acgaataaat
    11161 cgaccagacc cttcttgttc ttcaacgaaa aaggtaagaa aatgttcgat gctgtgtttc
    11221 tcgcttcctg gataactatc ccacaaaaaa gcaaaattat ccccttgata atgatagcta
    11281 tattctggaa aaacctcatc tacttgatag gtggagtgta cgtcaaataa aaatcggcca
    11341 ttttcttcta acgcttgata gacttcatca aacacttgct gtacctcttg tctatttttc
    11401 atatagcaca gcgaatcaga aaaacaggta attgcctgat attgtccgat ttctgataaa
    11461 tcgagcatat tgccttccac aaattggatc gatgcgtctt cttcatatgc tcgctggctg
    11521 gcaatcatca acatctcttc tgaaaggtct aaccctgtca ctttatatcc atcttttgca
    11581 aaggcaaccg ctaatgctcc agtaccgcat gccagttcca aaatattttt tgtgtcttct
    11641 tgtaaatgac gtttcgaaaa agctaaccat tgctgataaa gtgtttcgtc catcacttca
    11701 tcgtaaatga aagcaaatgt ttcataagcc atatgttaat ccaaccattc actaagatcg
    11761 actagcggag catctgacca tagtttttcc agattataga aatttcgttc tggcgcatgg
    11821 aaaacatgta cgatcacatc tccaagatcg atcaataccc attttccgcc ttcttttcct
    11881 tcaatacgtt ttacttcata tttgttttct tcttctttat caatgatttc ttctgtaatt
    11941 gcgttgattt gacgttcgct atttgctgaa cagatcatga aataatctgc caacaaggaa
    12001 acatttcgaa cgtctaaagc gataatgtct tctgcccgtt ttgaatctgc tgcttcaatc
    12061 gcaattttta attgttcttt actatctatg atgatttcct cctaatatga gcagcttatt
    12121 tttttgctac ccagtaatta tatgtttcga ttgtttttgg ataaatctgt tgttcttgcg
    12181 caagcaaatg ggccaatgta tgttttgttt caaatgctac agcttcgtct agatcagccc
    12241 atgcaatggc tcgtgcatct tgtaccccag gaaaatctcg acctggttca atatagtctg
    12301 ctacataaat gattttgtca aggagactca tcttagcagc cccagtcgta tgcacccgga
    12361 ttgcatgaag aatctctgca tcggtgattc ctaattcccg ctctacaaac gaagcgccaa
    12421 caagaccatg ccagattgca ttattataat tcaataactc cggatcaaaa ccgtctctta
    12481 cgattaccat tttgaactcc tcatctggtc gttcttttgc ataatcatgt gtcaatgcag
    12541 caatactagc tttttcagga gaagcaccgt attttttggc aagagccacg gctgtttctt
    12601 ctactccaag cacatgcttg aagcgttgtt cgctcattct catctgtatt ttttgcatca
    12661 ggttttctct agaataagca gtatattcat tactatagtc catattcata caacccttca
    12721 ttttgaatat aatcaattac tttatctgga acaaggtagc ggatagaaca gccttctttg
    12781 atcttttgac gtattttcgt ggaactgatg tcgatctcag gtacatctac ccaaattaca
    12841 ggatatggag tatccgttgt gtatcctggt cgacgtatac ccacaaagtt gaccatagaa
    12901 gtcagctcat cgattttata ccattttggc aaatattcta ccatatcccc accgataata
    12961 aagtaataat ctgtatccgg attgttttgc gtcaattcct tcatagtatc ataggtgtaa
    13021 cttttcccac cacgagctaa ttcaatcgtt tcaatctgca aaaaaggatt gtcttccacc
    13081 gctaattcca acatgttgag tcgatgtttt gcatcaatcg tctgtttctc atcaacatga
    13141 ggtggctgat aagaaggcat caaaaatacg cggtcaagac ctaaattccg gccagcttgt
    13201 tcagccatca ctaaatgtgc aaggtgaacg ggattgaatg ttccgcccaa gatccctact
    13261 tgctttttct tctctaagaa ttgaggcatt tcttcaggaa ataattgtga acgaacaaat
    13321 gtcttagctt gcgtattcat catggtcact ccttatattg cttttacttc ttttgatagc
    13381 ctttgatact tttcttttgt ggaaggtttg tataatacaa taacacgtcc aatgatttgg
    13441 acgatttcgc aatctatttc tttttctaat acttgagctg cttcttctgc aatctcatct
    13501 gtattttgaa gtaacgatac tttgatcagt tctctttttt ctaaagcttc ttcaatttga
    13561 acgatcattg ctgaattgat cccacctttt ccgatttgaa aaatcggctg aagatgatgc
    13621 gctttgctac gtaaaaaacg cttttgtttt cctcttaaac tcataatttt ccttcttcct
    13681 taaatcaatg ctttgcgttt caatacatct acaccctttg gcgcccagcc cgcaattact
    13741 actggcgctg ttacagtgat ccagcctaga ccggcaaaaa cgatgtctgt tttttctttc
    13801 acagaaaact caaaacgaac aagctccgga aatgcctcga cttcatcagg acgaggaggc
    13861 tgtaataatc cacctacatg tttttgataa aattcgtctg ctttttccag tttcgttcga
    13921 tgaatattta catcatttga cacataagca ataaatgaag cacgattgcc gctgataaaa
    13981 tcaaatcttg caagtccgcc caagaaaagt gtttgccctt cattcagctg ataaacttta
    14041 ggtttgattt cttttaacgg tgcaatgatc cgcaaatctt ttttacctaa gtagtgcgcc
    14101 atttgatggc gatggataat tcccggtgta tcaatcaaaa aatgcccgtc atctaaagga
    14161 atctcgattt tatccaaggt cgttcctgga aactgcgaag ttgtgattac atcttgtact
    14221 cccgctgtat ttttgatgat ttgattgata agcgtcgatt ttccgacatt tgttactccg
    14281 acgatataga catcccgtcc atcgcggtat tcttcgattt tttctaatag ttccgtcatc
    14341 tcatgagatt tcttagcact agttaataat acatctactg gacgaattcc tgcttcgtaa
    14401 gcacgttcct tcatccattg tgttaattta gtttttttca atgatttagg taatatatcc
    14461 actttgttcc caactaaaag tactggattg tcaccgacaa aacggtgtag tcctgggatt
    14521 agtgaaccat tgaagtcaaa aatatccacc acattgacga tcaatgcatc tttttgccct
    14581 aattcattca gtaagcgcaa aaagtcgtca tccgtcaatg agacatcttg gatctcatta
    14641 taatggcgta atctgaaaca acgctgacaa tagacttccc cggaagctaa acctttttcc
    14701 aaagccgttt gcggcgtgta tcctaattca ccgggtttgt ttgtttgtat aacggcacca
    14761 cagccgatac attgtaattc ttctgtcatt cgattccacc tcgccagccc atatcagggt
    14821 gttttgctaa tagataggcc ataattcttt tttcccaaaa acgattaaat tgtgtcttcc
    14881 aactatctgt tgcgataatg ggttttacaa ggatgctacg tatccctgca cgattggctc
    14941 cacgtatatc agtcataatc tgatcgccta ccattactat ctcatcatct gctaaatcca
    15001 attgtttctt tgctatgttg atccctcttg ctaaaggttt cattgctcgt gcaacataca
    15061 aaagatcaaa tttttcaatc gctcgcgcga cacgttttga attattgttt gaaacaacaa
    15121 caacagggat tcctgcattt ttcatttcta aaatccaagt aagcaactct tctgttccat
    15181 ctggattatt ccatgcaatc aacgtattgt ctaaatcggt caatactgct ttgatgccta
    15241 gattctttaa ttgggcaggt gtaatctgat agatggcttc taccatccat gttggtttgt
    15301 aatttgaaaa catgctgtct cctcatctgc aaaaaatgaa ggagcacata aagtactcct
    15361 caaccccgta ttatactgca tatcagcaaa aaaaactacc tctatactcg ttcgtctttc
    15421 agaaaaagca tttctttaca aaatgaccaa aaataaagat tgatcacaga aaaatttaga
    15481 aacaaaaaac aactaatatt ggcggaacct cttgtatcgt ttttccagta atttatgctg
    15541 atctaatcga acaagttccg tcaatctttc aatcaacgct tttttcataa tgtgtgcgac
    15601 tttatatcgt ggcaactgct cgccgttgaa cgtttcagga ataactcgat caacgattcc
    15661 taactcgcgt agttctggcg cagtaatttt catcaactca gctgcttctg cggcgcgact
    15721 accgtctttc cacaaaatcg acgcaaatcc ttctggagaa agcacagcat aaatcgtttc
    15781 ttccagcatc cagacttcat ccgctaaacc tagagcaagg gcaccgccac ttccaccttc
    15841 accaatgatg atggcgatga tcggtacctt taaatcagac atttcgagca gatttctggc
    15901 aatagcctct ccttcgccgc gctcttctgc tcctacaccg caatatgcac ctgctgtatt
    15961 gatcaaggta atcaccggtc tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt
    16021 tcgatagcct tcaggatggg gagaaccaaa atttctgcgg atattttcct gcagatcccg
    16081 tcctttttga ataccgatca ctgttaccgg tttgccatct aatcgagcga ttccaccaac
    16141 aatcgcttcg tcatctgcaa ataaacgatc accatggcat tcctcgaaag aatcgaagat
    16201 ctcttcaaaa aaatccaagc ttgtccatcg ttctttagct cttgctaact gaacgacatc
    16261 atgtgctgtc ttatccacga tctatccatc ctttctgtgt atgcaattca agaagcgtat
    16321 ggattttctg tttcaattcc atgcggggaa caatctgatc gacaaaacca tgagataata
    16381 agaattcagc cttttggaaa tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc
    16441 gtcttccagc aaaaccgatc aagctttggg gttctgctag aataatatcg ccttccattg
    16501 caaaactggc agttactcct ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac
    16561 cttcattgct atgccgtttg acagcagcag agatttttgc catctgcatt agagaaaaga
    16621 ttccttcttg catccgtgcg ccacctgaag ctgtgaacaa gacgactgga agactttcct
    16681 ttgttgctcg ttcgaataat ctggtgatct tttctccgac aatcgttccc atacttccca
    16741 tgataaaatt aggatccatc actccaataa tgaagggaat cccctgaatg gttgctttac
    16801 cagtgaggac tgcttcatga agtcctgttt tatcttgcat ttttttaatt ttttccgtat
    16861 attcgggaaa tgctagcgga tctttggtta caagatccgt atcccattct tcaaaacttt
    16921 tttcatcaat aatcaaagct agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat
    16981 gagggcatac tttttcttca ccgatgtctt tcgtataaag gatatgtttg caatttggac
    17041 actttgccca catgttatca ggaacagatg gcgagttagc aggtttgctc tctctattag
    17101 gattgatccg aatatatttc tttttcttaa acagagccat ctatgcgcct cctatctccg
    17161 tttctggtgt ccaattcggt aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg
    17221 ctatgactgc aggatgacta atcaaatcca tctgaaattc tgcgttcgtg atgattcctt
    17281 ctgtgataaa ttcacttaag gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc
    17341 catggacgat tatttttgca atcattgaat cataatatgg cgggatcgta tatcccgggt
    17401 aaactgcact gtctactcgc agtcccatcc cacctgctgg aagcagtaag ttttggatct
    17461 tccctggaga aggagcaaaa tggaaagcag gattttctgc attaatccgg cattcgattg
    17521 cgtgtccttg gaatactact tcttcttgac acacattcaa aggctcaccg gctgctatct
    17581 ccacctgttt ttttactaaa tcgattcctg tgaccatttc tgttactgga tgttccactt
    17641 ggattcgagt attcatttcc atgaaataaa aatcgccttc ttcatccatc agaaactcga
    17701 tcgttccggc attttcataa ttgactgctt tagctgctcg aacagcagcg tctcctagtg
    17761 cttctctttt tgtttgtgag atcactacag aaggagattc ttccagtact ttttgattgt
    17821 tgcgttgtaa agaacaatct cgctcgccta aatgaatgac atgaccaaat cgatcaccta
    17881 agatctgaac ttctatatgg cgagcaggat aaatgatttt ttccaaatac atatcatcat
    17941 ttccaaatgc cgctgctgct tcttgctgag ctgaagaaaa atgctgtggg agctcttctt
    18001 ttgattgaac tttccgaatg cctttcccgc caccacctgc agctgctttc agcatcactg
    18061 gataaccaat tctgtctgct atttccaatg cttcttctac tgtagataat acaccagtac
    18121 ttcctggaat gactgggaca tttgcttctt gcatcaattg acgcgcattg atcttatttc
    18181 ccattgcatc gatcgttgca cttttaggac caataaatgt gatattgcac tcttcacaca
    18241 tggaggcaaa tcggctattt tcagataaaa aaccaaatcc tggatgaatt gcttcggctt
    18301 ttgtcacgat tgcagcgctc agtacttcct gaacattcag atatgaatca acggcttttg
    18361 ccgggccaat gcagattgct tcatctgcta actgtgtatg aagtgcttcg gcatctgctt
    18421 ctgaatatat cgctacggtt tggatcccta gttcgcggca tgcacgaatg atacgtacag
    18481 cgatttcacc gcggttagct atcaatactt tagaaaacat atttcaccta tccaatcata
    18541 aaggttaatt ccgcttcggc tactttttta ccgtctactt tagcgattcc tttgcctatt
    18601 ccggctgaag ctttcacttt caatatctca acttcaaggt ataaggtatc tcccggtgta
    18661 actttcttac ggaattttgc tttgtccaat ccgccgaaat aagccgtctt tcccttgaat
    18721 tgttcaagag aaagtaatgc aactgctcct gcttgagcca ttgcttcaac aatcaatacc
    18781 cctggcataa caggttcttc tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca
    18841 tttttcttag caacgatccg ttctccttca accatttctt ccactcgatc aatcaaaagc
    18901 attggataac gatgtggaat gatttctttt atttcttgta tgttcattgt tttccctctt
    18961 tcctgctttg cttatttttg ctgaatccta aagagtggct gtccatattc gacaactgac
    19021 tcgttttcta caaggatttc tgtgatcact ccgtcggcct cactagttat ctcattcatc
    19081 agtttcatcg cttcaataat gcagataact tctcctttgg caacttgatc acctactttt
    19141 ttgaaagctg gtttgtcggg cgctggttgc agataaacga ttcccacgat tggcgaaaca
    19201 acctcgattc catcaaatac tgccgattct ttttctttca ttatttctgg agcagttgtt
    19261 gcttgttcag atatgacatt gactggctcc gaaactggca acgtttcgtt tgtttgaacc
    19321 atattcatgc ctcgacttgt tttattttta ttcatataca attcaaattg accttcacgc
    19381 aaatcgaatt ccgttagcga ggattgatca aattgactga ctaattctct gatttcattg
    19441 atatccaaaa ttattcctcc cagcgtttca tgcacagaac cgcattatgt ccgccaaacc
    19501 ctaatgaatt actcattgca taagtaaaac ttgcaggttt gctttcgttt ttgacaactg
    19561 tcaagtcaat ctcaggatct tgtttttcta cattgatcgt aggtgggata aattgatgtt
    19621 ctaatgccag caaagttgcc aaggcttcga tcccgccggc tgctcccaat aaatgtccag
    19681 tcatcgattt cgtgctgctg acgtaagtat ctctgaaatt ttcacctaat gcgatttgga
    19741 ttgcttttgc ttctgcctgg tcattggctg gtgtacttgt tccgtgagca ttgatatatc
    19801 cgacttgttc cggctgtaca ccagcttctt ccatcgcaag cttcattgct ttagatgcac
    19861 cagaaccatc tggagtagga gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa
    19921 cgatttctcc atagattttg gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta
    19981 gaacgcctgc tccttcaccc atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac
    20041 cacgattagg atcagttgat tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca
    20101 tctcacaaat cgttgcttct gttcctccag caagaatgac atcagaatag ccatgtttga
    20161 tgttacggaa tgcttcccca attgaattcg ttccgctagc acaagctgtt acgatagctg
    20221 tacaaatacc ttttgcacct acacgcaaag caatatttcc agctgccatg ttggcaatcg
    20281 ccattggtac gaacattgga gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt
    20341 ggttttcgat tgttggaaga ccaccgatcc cagatccgat gatgacgcca aaccggtctt
    20401 gattcatttt ttctttatct aaaccgctca tctccattgc ttctaaagca gcgtagattc
    20461 catagatcga aaacatatcc attcgtttgc tatctttttt aataaaatat ttatcgtaag
    20521 ggaaatcttt gacttcaccc gctaaggtaa tccctgtttc tgaagcatca aatttagtga
    20581 tcggtgcgat gccattcgtc ccctctttta agctattcaa aaagctgtct gcgtcatttc
    20641 caataggtga agtgactcca tagccagtaa ttaccactcg attcatttgt ttgctctcct
    20701 tttcttatcc gtgcatgacc aatccgccat cgacattgat gacttgtccg gtgatatatg
    20761 gattttgtgc taagaaaact gctgtttgtg caatatcttc tacttgtccg aaacgctgca
    20821 tcggaatttg cttttccgct tgttccttga ctttgtctgc taaaacttcc gtcatatcag
    20881 ttgtgatgaa tcctggcgca atggcattac atgtaatgcc tctagttgca gcttctcgtg
    20941 ccacagattt tgttaatcct actacgccag ctttactagc tgcataattt gcttgaccaa
    21001 tatttccaat caacccagat acactagaga gattaatgat ggccccttct cgctgcttca
    21061 tcattttttt caatacgtgc tgcgtcatat tgaaggttcc aatcaaattg atatccagac
    21121 attttttgaa atcttctgca tccattcgca tcacaagttt gtcatttgtg attccggcat
    21181 tgtttactaa cacatggatc gatccaagct tttcttctgc ttctttgatc atttgtcccg
    21241 ctttctcata atcagagatg tcaccggaaa ctcctacgca ttttacaccg aatgcttcga
    21301 tttcttctat cttttctttt ggaatctctc cgcgaccatt caaaatgata tttgctccag
    21361 cttttgcaaa agctaaggcc atcgctttgc cgattcctct tgtactacct gtgataaata
    21421 cattttttcc ttttacatcc ataacgttcc tccttatagt attgctattg tttcatcaaa
    21481 tgtctgctta ttctcaacac gcagaactgg tattgtttta tcgatttttt tcataaatcc
    21541 gcttaatact tttccggggc cgacttcgat gacttgttcg atgccgattg tttttaactt
    21601 atggatactc tcatagaaac gaacaggttg catgacttgc tgttcaagta gtcccgcgat
    21661 tgtttctttc ttcattattt ccgttgttgt gttgcttatg ataggaaaag aaggttcaga
    21721 gaattgaatc tgacttaggt ccttcgccag tttttttgct gccggttcta aaatagctgt
    21781 atgaaaaggc ccgctgacat ttagagggat cattcttttg aacccttttt cttttaatag
    21841 taaaacagct tcgtctactg ctttttcttc gccaccaatc acgatctgct gaggtgtatt
    21901 ataatttgca ggagatacga tgccgtactt gctagcttca tgacagcttt cctcgatcgt
    21961 ttcaatcggc gcattcatca ctgcaaccat tttcccgctt ccagctgggg tagcttctgt
    22021 catataagcg cctctttttg ctactaacgc aactgcttca gtgaaggata gcgcattact
    22081 agctactaat gccgaatatt ctcctaaact cagtcctaaa gctgcatctg ggatgattcc
    22141 atgctccttc aataagcgat aatacgcgat gctgactgtt agaatagctg gctgtgtata
    22201 ttgcgtctga tccaaacgtt cattttctgt aaagcaaagt tcagccatat cgtagccgag
    22261 aatctcactt gcttcatcaa atgtctgctt tacaacggct tcttcatata aatcttttcc
    22321 cataccttga tattgtgcac cttggccact aaataagaat gctgttttca tgtttttctc
    22381 caatatatcc agaatatatg tttgattctt cctattcaaa aaggccgaga cattcctatt
    22441 tcaggaaatg tcccagcccc caacctaatt ttaatgatta tgcagtttgt ttttctacgt
    22501 aagtcactaa atcttgaacc gttttgattc cgtcttctgt ttcgattttc acgtcaaatt
    22561 catcttcaat ttcattgatg atttggaata aatccaagct gtcagcttct aagtcttctt
    22621 ggatgtcagt agtcaatttg atttcttcag tttcttttcc taattcttct gcgatgattt
    22681 cttgaatttt ttcgaatacc ataataatat attcctccga tattgtttgc tttttatttt
    22741 tataatttaa cagtctacaa ctccatgaga atacttcccc atgttaaacc tccgccatag
    22801 cctgtaaata ttactttctg ctgttttcct aaggtgatct ttccactaga aactgcttca
    22861 tccaataaga tagggatgct tgcagcagat gtattcccat atttatccat attggtcagg
    22921 aacttttctt gcggcatctt tacttttcgg gcaattttct caataattct gaggtttgcc
    22981 tgatgcaaaa gcagataatc aactggtttg tctttcatga cttcttggat attgtttgca
    23041 acgtctcgta cggcaaaatc aaaaatatct ctgccagtca tttgcaaata atacgaacct
    23101 tctgaatctt ctgaatagaa cggattttta ttttgctggt agccagcggt caatgagcta
    23161 cttctggtac cgtcagctgc taatttttct ttcagcaatt ttttctgtgg attcgcttct
    23221 aagagaatcc cacctgctcc atcgccaaac aacacagcag tcgtacgatc agaccaatcg
    23281 attagcttag atagtgtttc accaccaatc accagtccct tagttgagcc gcagcggatc
    23341 attttttcag ccatgcttaa cgcataaaca aatcctgagc acgcagcact gatatcaaaa
    23401 gcgatagcat gagttgctcc caatttccct tgtacttgtg ctgctacaga tggtgtcgtg
    23461 taatcgggcg acattgttgc tactaaaata aaatccaaat ctttagggtt tagatttttc
    23521 tgttttatca atttttccgc cactaaaatg cataaatcag aagtctcctg atttgttaca
    23581 catcttcgtt cacgaatacc cgtccttgac gaaatccatt catcagaggt atccatcatc
    23641 ttggaaagat cattattagt aataatcttt tcaggaagga cgctggctgt tgcagtaatc
    23701 attccaaatt gttccattaa atatcctcac ttatagtctt gcaagaagtc gtgcaagttt
    23761 ttcagtgcat ccagcaaggc atgctgttcc tccgtagcca ttccgtctaa gatgttcttg
    23821 accatttcac gatgaaaatg ctgatggacc cgaaatagta actttccttt tttcgttagt
    23881 cccaacttta caacgcggcg gtcatcttca ctgcggatac gttcgacata gccttttttt
    23941 actaacttgt taatagcagt ggtcaaggtc ccaacagtaa ttgatagttc tttggctact
    24001 tctgaagttg tttttttctt gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt
    24061 gacagatctt tgaattgaga ttttttcagc tcagactctt cgattgtcaa aatatcattg
    24121 aagacactaa caagataatc atttactgtt tttaaatctg gttccatgtt ccaccgtcca
    24181 ctaattagtt tgattatcaa atcttttgat gttcaaacta tatcttaact tttttcaaaa
    24241 tgcaacctta aaagatcatt tttttactat atgagctttt tttaagattt tactcattta
    24301 aaacaactga taaatcagca tttataattt cttttattag aaaaatcatc tatctcacat
    24361 attttgatta tcaaagtatt ttctgtgaac agaactatag aattccctaa aacttgtcca
    24421 ttatttcaaa atttgttata gtctttttat tcaaagcgta aaaggagatc ctatgttaaa
    24481 agaaaaaatg aaaactctcc ccatgctgcc gggtgtctat cttatgaaag accgagatgg
    24541 taaggtcatc tatgttggta aagcaaaacg gctcaaaaat agagtaagca gctattttca
    24601 tcagaataaa cagcattcaa aaaaagtatt acgtatgatt catcatataa cagattttga
    24661 tttcgtcgta gtagacacgg aattagatgc tttattgctc gagtgtcaat tgatccaaca
    24721 ttaccgtcct ttttataacc gacaaatgaa ttatttcagt aattataatt atgtacatat
    24781 taccaataag ggatttgtat taacagatac tcctacggct cgaacatacg ggccttttcg
    24841 tctctataaa aaaatgccgt ctattttgcg tataatggaa gaaacttatc aaatgccttg
    24901 gttgtctgaa attagtttgt tagctttgcg agtccagctt cctgacctac aggaaatgac
    24961 ttttgaacag aaaaaaaagg aactccaagg gctgtttcaa ggccgaaata aaaagctact
    25021 gacttacttg aaaaaaagac aacaacattt catctatcag ttgaatttcg aaaaagccgg
    25081 tatgcttcaa aaagatatcg aattagttac ttattttatt agacggatcc aggaacaaaa
    25141 gcagttttta agaacaccat cgctcacctt cagcatgcca ttagcagcag atgagagtca
    25201 gaaaaagcac tacctcgttt gttatggtca acttgcagaa acaatgatta cctctggtga
    25261 cgtttctcct gacttctatt atgaaaagaa agaagctcat ttatctttga aacgtcaatt
    25321 gtcaaaagaa gaaatcgatc ctgtacaaat cttgatcagt taccgcaaaa aattagaaaa
    25381 agaacagttt gaaattgaac tgttaaacaa aaaagaagct gaaaagcaac taaattagtt
    25441 gcttctcggc ttcttttagg catcttcaac ttcaaatgtc aattatccta aaatttattg
    25501 gattaagtca tagatttcca tcgctacgat atcgatatta tcaaattcgt attgctgatg
    25561 agtatctgtt tcagttaatt caaatgtatc ggtcgcttta tcataagtta ctgtacactt
    25621 ctctacgcct tctttttcaa aacggcgaac ttgcagttca ttatctgctg actcgaccat
    25681 agcctccagt cgttttatga ttgcgactaa ttgtgaatgt gtcatcggag gtcctccttt
    25741 tcttatttct gttatattgt atcaaaagca tagcctactt gcattgattt tctctaaaaa
    25801 aactcgtttt ttatgatttt ttttctggta tttttccttc aggccaccaa agtttgatca
    25861 aagcttgggt tccgtcattc tcaaaacctt tatctgccaa ctcttcatat agtttcaatg
    25921 cttgggtagt tgctggcaat acaagatcca ttttttctgc ttcttctaat gcaatcttta
    25981 aatctttgat aaaatgtttg acaaaaaaac caggtgtgta atcttctttt aaaatacgag
    26041 gagaataatt gcttaaagac cagttagctg cacttccacc tgaaagcgtc tcaatcactt
    26101 tctttaaatc aagtccgttt ttttgtgcat agacaagcat ttccgtcatt cctgtcatcg
    26161 tacctgcaat catgatctga ttagccattt ttgtatgctg acctttccca gcagaaccat
    26221 gcaatgtaaa tgtcgtacca aattctttga ataaaggcag tacttgatca taaacggctt
    26281 cttctccacc gaccatgatc gtcagtgtac cattctttgc tcctaagtca ccaccggata
    26341 ccggtgcatc taacgcatct gcgccttttt cttttgctgt ttttgcaatt ttttctgcca
    26401 ggcttggtgt acttgtagtc aaatcaacta aaatatgtcc tgagatatct gcagaaaaaa
    26461 tccccgactg tccatagtag atttcttcta catctttggg gtaacctacc attgtaaaga
    26521 gaatattact ttgttctgct accgcttttg gtgtatctgc ccagatagct ccttctgtta
    26581 ctaaaggatc agcttttgct tttgtccgat tatagacagc tacttcgtgt cctgcttcta
    26641 gtagatggcg tgcgacagca cttcccatga cgcctgttcc gataaaacca agtttcattt
    26701 tttcgacctc ctaaaaatca aaaatccttg cttctatgct agtatacgct agaaacaagg
    26761 attttttcat aaaaaatgtt tgaattttat cttttaaatg aatttaattt tattttgatt
    26821 cgttcaatcg agtcttcttc ttcttttctt cgcatctctt cttcatgata aacaaccatg
    26881 tcttgataaa attcgatcat agcttcgccg aaatgtgtag atgaaatatc ataaagttta
    26941 gcttctaaaa cttttggatc tttaggaact tcactggaaa tataatcaat aagtgctggt
    27001 gcaaagtctt cgtcggtttc aaaagttttc cctaatgaag gatgatcgaa caatcggttc
    27061 aaataagcat ttccttccac gacacaaggt gttccggctg ccatcgcttc tgtataagta
    27121 agcccttgtg tttctgaagt agaagcactg acaaaataat ctgccgcttt ataataaagt
    27181 gctacatcat tgttgtccac ttctcctgtg aactgcacaa attcttctaa ctgcagttct
    27241 gagatcaatt cctctaactc ttctacataa ggtccttttc ctacgataac taatctggcg
    27301 ttaggataag cggagatgac ctgaggaaac ccattaatca aggcttggat atttttctca
    27361 taagaaatcc tgcttaaaga aagaatcatc aaatggtttt cctgaagacc tagtgactcc
    27421 cttaatttgc tgatatcctg ctgagtgata tcagaacgtt tgaatttttc aatgtcgata
    27481 ccagtaggaa tcacgcgaag cggtgctatt acaccatagc tgcgtaatgt atcaataaca
    27541 cgttcacttg gacaaactac acctgtcgta tgatttgcaa ataatctaga caagtatttt
    27601 acatgggatt gcctgatcac ttttccttta ccgatataat gaaggtagtc ttcatacatc
    27661 gtatggtaag tatggatcag aggtattttc aacttcttag ctaccatctt cccaagtatc
    27721 cctgctccga attctgtatg ggtatgaata agatctagtt ctagttcttt ggcaaccaaa
    27781 taagcatacc acatgcctct aacaacaact cttcgatcct tgaaagaaat aaatggtaca
    27841 cttggcattc gaatcacatc tttttccaac ttatccgcat taggatcggt cgttgtaaaa
    27901 atatagactt catgcccatg tctttctaat tcttgtttta atgttttgat cgatgtagca
    27961 actccgctga cttgcggaaa gtaggtatcg gtaaaaaagc cgattttcat gtttttcgcc
    28021 tccaaactag tccttgacct gatttacttt ttttaatttg ctagagactt tttcattcag
    28081 tacactttcg tatacatttt taagttcttc accgatttta ttgatacttc gctcttttgc
    28141 tgtttgataa ccggcttgtg tcgtactcgg tagttcttga ttggtaatct gttggatcag
    28201 tcggataaat tcttcgtttg tcttccccat atagcagttc ttgctatctt ctaaccagcc
    28261 attatacact ggaatatctc gaacaagtac gttttgtttg cttgctaaag cttctaaaac
    28321 aacgatccct tctgtctctt cataagaagg gaaaaagaat agatctgcac ctgaatacgc
    28381 accttctatg atctctccac gaatgtaacc aggaaacagg acattttcag gatgatcttc
    28441 ttttacaatt ttccggatgt tccgaggaat cgagtacatg ggaacatgac caaaccaaat
    28501 aaatgtgtat tcgggcatct tcttagcaat ttctacgaaa tcaatgattc ctttacgttc
    28561 gaaaaacaaa ccgacacaaa tgattacttt ttgatccgga gacagtttga aatattctct
    28621 gaacttttgt tctttcattg gatcagggtg atatttctct aagtcgatcc cattggaaat
    28681 agcttgaatc ggcacacgga taccatagct ttccaacaac tgttttgaat aaggtgttgg
    28741 cgtgatcaga tagtccgctt tttggtacaa tcctaccaga tattttttta cgataggaga
    28801 aagctggttc gatccgataa aggaatttcg gaaatcttcc tctgtagaat gcgcatgata
    28861 gacaactttt tttccttctc ttctggcttt attgaccata ttatgactat tgattccgta
    28921 tgtattgatg tgcaggatgt cataatcttc tttttcatct agtgtataag agataccgac
    28981 ttccgtcaga gcacgttttt gatgatcaag cgcacgcccg attccagatt ttgcaagcat
    29041 cttttcactt tcaaaatata ataatacctt caccttacgt cccccttcac ttatatagta
    29101 taacatagcc tacaaactgt cactcaaact taagccctat ttttgtctat tcttactgac
    29161 aaaaacgttt tcttaaaaaa gaacaaaaaa actggcgtag ctcttttaca agccacacca
    29221 gcttttgata cgttgtttat tttacatatt catgaacaag ttcaacgact tcttccatca
    29281 tatcgcattc tttcaatgca cgatcagcaa gttcagccat tttcgctgtg tccaagcgtt
    29341 tcattaaact acgcgtttta agaatagatg tcgcactcat tgagaactca tctagaccca
    29401 tacctactag caatggaaca gctgtttgat ctccagccat ttctccacac atacctgccc
    29461 attttccttc agcgtgtgct gcatcaatta catttttgat caaacgtaaa attgatgggt
    29521 tatatggttg gtacaagtaa gaaacgcgtt cgttcatacg gtctgctgcc attgtgtatt
    29581 gaatcaaatc gttcgtacct acagagaaga aatctacttc ttttgcaaac ttgtctgcta
    29641 agactgctgc tgctggaatc tcgatcatga tgccgacttg gatcgtatca gatacttcct
    29701 tgccttcact tatcaatttt tgtttttctt cttcaaagat cgcttttgct gcgcggaatt
    29761 ctttgagtgt agctaccata gggaacatga tacgtaaatt accatgaaca gatgcacgaa
    29821 gcaatgcacg catttgtgta cggaacattt cgtcgccttg ttcagataaa ctgatacgca
    29881 atgcacgata tcctaagaat ggattcattt catgaggtaa ttgcagatat ggtagttctt
    29941 tatctccacc gatgtccatt gtacgaacaa ctacaggttt tccttccata ccttctaata
    30001 cagctttgta agcttcatat tggtcatctt ctgttgggaa gtctggagaa tccatataca
    30061 ggaattcagt acggtacaga ccaactgctt ctccaccatt attatggaca ccaactagat
    30121 cttttggtgt tccgatgttt gcagctaatt caaaatgctt gccgtcagct gtgacagttt
    30181 ctgcattttt taatttttcc cattctgctt tttgtgctgc gtaatctgca cctgcttttt
    30241 caaattcagc tttttgttca tctgtaggat cgatgattac atcgccttcg atcccgttga
    30301 cagccaaaat atcgccttct ttgactttag cagtaatttc ttttgtccca acgattgctg
    30361 gaatttctaa agaacgtgcc atgatagcag aatgtgaagt acgtccacca atgtctgtta
    30421 caaacgcttt tacaaaattg cgatctaatt gtgctgtatc acttggagtc aagtcatgtg
    30481 caacaacgac cacttcttcg ttgatcattg atggatttgg taaagtaacg cctaataagt
    30541 gcgctaaaat acgtttagca acgtcacgaa tatctgcagc acgctcttgc atataagcat
    30601 tatcttccat tgcttcgaac ataccgatat acatatccgt tacttctttt agcgctgatt
    30661 cagcatttac gctgttgtct ttaatatttt gcttgatttg acctaccatt tctggatctg
    30721 aaagaaccat caaatgggca tcaaatactt gcgcttctgc ttcacctaag ctttgcgctg
    30781 ctttttcacg aatttgctgc aattcttcag tagatttagc taatgcagca tccaaacgag
    30841 cttcttcagc cgcagtatct tctactgttg ccttactgaa tgttaaatcc ggttgaacta
    30901 gcaggtaagc ttttgcaacg gctacaccgt cactagcggc aatccctttt agcatttcaa
    30961 ccattattca gataagcctt ctttcttcat tgtttcaacg attgctgcca ttgcatcagc
    31021 ttcgtcagca ccttctgcag taatagtaac atcagaacct tgacctacac ctaaagacat
    31081 aacgcccatg attgatttca agtttactga tttaccttta tactctaagt ttacatcaga
    31141 gttaaatttg cttgctgttt gtactaatag tgtagctgga cgagcgtgga tcccagtttc
    31201 tgctactacg tgaaattctt tcttttccat attaatcggt ctcctttaag tgagtgttgt
    31261 ttttatgtga gggttttctt ttttagtaat tcagagaatc ctaacaaatg ccaccctttc
    31321 atctgtaaag attaacattt tttttcgtta gatacaacag ttttcattca atttcagata
    31381 aaagtattca aattattccg gattcttttt catccttttc ttcacatttt ataacaaacg
    31441 tgtttatttc ctttttcttg tcgctttctc ttctcctgac gtttatcatt ctttcctttg
    31501 accctctttg ttttcatatg aaacgaatta cttgcttccc tttttatttt tagtataata
    31561 agagtcaaag gtcaggaatg gtcaatagag atttcacctt ttctacctca taaatctctt
    31621 aaaaaactag gggttattct tttacctcat gaaaaacgag atgtaaaata tggatgtacc
    31681 catttttgtg aaaggacgtg aatgattcat gctttgtcaa aactgcggca agaatgaggc
    31741 aacgattcac ttgtatgcca ctgtcaatgg tcagcggaca caactcgatt actgtcagag
    31801 ctgttatcaa aaaatcaaaa atcaacaaaa tggaggtctg actaatatgg cccaaaatga
    31861 tccatttgga tttggcagtt tggacgatct tttccgttcc atgtctcgcc aaatgcaaca
    31921 gcaaggaaat tatgagcaaa caccaccaac acaatttggc ggaaacaaca acttcaatgg
    31981 tggtcagcca cctcaaggca atacagaagg tttattggga gaatacggaa tcaatatcac
    32041 agaacaagca cgacaaggtg atatcgatcc tgtgatcgga cgcgatgacg aaatcaaacg
    32101 agtcattgaa attttaaatc gccgaaccaa gaacaatcct gtcttgattg gagaaccagg
    32161 tgttggtaaa acagcagttg ttgaaggttt agcacaaaaa atcgtagatg gtgatgtacc
    32221 gcaaaaatta atggacaaag aagtcattcg tttagatgtc gtatctttag tccaaggtac
    32281 tggtatacga ggacaatttg aagaacgtat gcaaaaattg attgaagaga tccgtcaagc
    32341 agaaaatgtg atcctattca tagatgaagt tcacgaaatc gtcggtgctg gttctgctgg
    32401 tgatggaaat atggatgctg gtaatatttt aaaaccagct ttagctcgag gagaattgca
    32461 aatggttggt gctactacct taaatgagta ccgcatcatc gaaaaagacg ctgctttaga
    32521 acgtcgtatg caacctgtac gggtagatga accaactgtc gaagaaacga tcagtatttt
    32581 gaaaggtttg caaaaacgtt atgaagacta tcatcatgta aaatatactg atgaggccat
    32641 cgaagcagcc gcaactttat ccaatcgtta cgtccaagat cgcttcttgc cggataaagc
    32701 catcgatttg ctagatgaaa caggttctaa gaaaaacttg acgatccaaa tcgttgatcc
    32761 taaaacaatc gagaaaaaac tgcaagaagc agaagaacaa aaagttttag cttctcgaga
    32821 agaagatttc gaaaaagcag cttattatcg cgatcagatc aataaacttc aaaaaatgaa
    32881 agaacgtcag ctcactgaag aggaaacacc tgtgattacc gaaaaagata tggaaaaaat
    32941 cgttgaacaa cggacaggta ttccagtggg tgaattaaaa gaaaaagaac agacacaact
    33001 gaaaaactta gcagatgact tgaaagctca tgtcatcgga caagataacg ctgtagatcg
    33061 agtagcgaaa gcgattcgcc gaaaccgtgt tggattaaac aaacagaatc gtccaatcgg
    33121 ctccttctta tttgttggac caactggtgt aggtaaaaca gagcttgcga aacaacttgc
    33181 ctacgaacta ttcggttcac aagattcaat gattcgtttc gatatgtctg aatatatgga
    33241 aaaacatagt gtctctaaat tgattggttc tcctccaggt tatgtcggat acgaagaagc
    33301 tggtcaatta acagaaaaag tacggagaaa tccttacagt cttgtcttgt tagatgaagt
    33361 agaaaaagct catccagatg ttttgcatat gttcttgcaa atcttagatg atggtcgatt
    33421 gacagatgca caaggaagaa cggtcagctt taaagatacg atcatcatta tgacaagtaa
    33481 tgccggaact ggtaaagtag aagcaaacgt tggttttggt gcggcacgcg aaggtgttac
    33541 tcgttctgta ttgaaccaat taaacaacta ctttacacca gaatttctga accgttttga
    33601 cgggatcatc gaattcagtg cattatcaaa agaaaacttg atgacaatcg tcacactaat
    33661 gctagacgat gtcaaccaaa tgctggcagc acagcaactt cacatcgaag ttcctacgaa
    33721 tgtcaaagaa aaattagttg acttaggcta tgatccatct atgggtgctc gtcctcttcg
    33781 acgcacgatc caagaacaaa tcgaagatgg tatcgctgaa ttttatcttg atcacccttc
    33841 tatccatgag ttgaaagcaa aactggataa agatggaaaa atcatcgtta cttctaaacc
    33901 agaacgtttg gctaaagaat cagccgaaga aacggctgaa taaatccagt tttatagcgt
    33961 gagacggtat ttctttgaga aatgccgtct cattcttttt tcagaaaagt caaaatactg
    34021 ttcattttat aaaaaagaaa ttataataag agaaacaagc aattaaaaat acataaaaga
    34081 ggtgagcgcc atgaaattgg taaatgtaac aaacagccat tcccgtcttg tattaaacca
    34141 gcttgaaaac actgatgccc atctagtcaa ggtctacaca gccggaaata ccactattgt
    34201 ctacactgaa gcccctgaac ataatgaaat tctgctgatc aatgacaaac gtaaaatcca
    34261 gccgaaagaa atcgaagatg ctaaaaatta tttcttagat aaaatcaaag acgccgttta
    34321 ccacgaggat gaaatcaaag taatcgaatt ggcaggcctt gttgaaatct cgattccaaa
    34381 acatgaatta ggcgctacat tagccgtgta aacaaatttt gaacataata at
//
LOCUS       DACIRY010000019        33590 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.019, whole genome shotgun sequence.
ACCESSION   DACIRY010000019 DACIRY010000000
VERSION     DACIRY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.019"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            194..2101
                     /locus_tag="IYE22_000750"
     CDS             194..2101
                     /locus_tag="IYE22_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ9505983.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2642..5920)
                     /locus_tag="IYE22_000751"
     CDS             complement(2642..5920)
                     /locus_tag="IYE22_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ9505984.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(6191..6736)
                     /locus_tag="IYE22_000752"
     CDS             complement(6191..6736)
                     /locus_tag="IYE22_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9505985.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(6748..7440)
                     /locus_tag="IYE22_000753"
     CDS             complement(6748..7440)
                     /locus_tag="IYE22_000753"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ9505986.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(7459..7743)
                     /locus_tag="IYE22_000754"
     CDS             complement(7459..7743)
                     /locus_tag="IYE22_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9505987.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(7756..8319)
                     /locus_tag="IYE22_000755"
     CDS             complement(7756..8319)
                     /locus_tag="IYE22_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ9505988.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            8547..9209
                     /locus_tag="IYE22_000756"
     CDS             8547..9209
                     /locus_tag="IYE22_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ9505989.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            9333..10529
                     /locus_tag="IYE22_000757"
     CDS             9333..10529
                     /locus_tag="IYE22_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ9505990.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(10630..12006)
                     /locus_tag="IYE22_000758"
     CDS             complement(10630..12006)
                     /locus_tag="IYE22_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9505991.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(12043..12909)
                     /locus_tag="IYE22_000759"
     CDS             complement(12043..12909)
                     /locus_tag="IYE22_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ9505992.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(12998..14023)
                     /locus_tag="IYE22_000760"
     CDS             complement(12998..14023)
                     /locus_tag="IYE22_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9505993.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(14041..14715)
                     /locus_tag="IYE22_000761"
     CDS             complement(14041..14715)
                     /locus_tag="IYE22_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9505994.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(14899..15792)
                     /locus_tag="IYE22_000762"
     CDS             complement(14899..15792)
                     /locus_tag="IYE22_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9505995.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(15866..17563)
                     /locus_tag="IYE22_000763"
     CDS             complement(15866..17563)
                     /locus_tag="IYE22_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ9505996.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(17556..18869)
                     /locus_tag="IYE22_000764"
     CDS             complement(17556..18869)
                     /locus_tag="IYE22_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9505997.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(18869..22906)
                     /locus_tag="IYE22_000765"
     CDS             complement(18869..22906)
                     /locus_tag="IYE22_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ9505998.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(22919..23377)
                     /locus_tag="IYE22_000766"
     CDS             complement(22919..23377)
                     /locus_tag="IYE22_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9505999.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(23675..24193)
                     /locus_tag="IYE22_000767"
     CDS             complement(23675..24193)
                     /locus_tag="IYE22_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ9506000.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(24195..24812)
                     /locus_tag="IYE22_000768"
     CDS             complement(24195..24812)
                     /locus_tag="IYE22_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ9506001.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(24809..25663)
                     /locus_tag="IYE22_000769"
     CDS             complement(24809..25663)
                     /locus_tag="IYE22_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9506002.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(25660..26571)
                     /locus_tag="IYE22_000770"
     CDS             complement(25660..26571)
                     /locus_tag="IYE22_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9506003.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(26612..27946)
                     /locus_tag="IYE22_000771"
     CDS             complement(26612..27946)
                     /locus_tag="IYE22_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9506004.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            28154..28984
                     /locus_tag="IYE22_000772"
     CDS             28154..28984
                     /locus_tag="IYE22_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ9506005.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(29228..29477)
                     /locus_tag="IYE22_000773"
                     /pseudo
     CDS             complement(29228..29477)
                     /locus_tag="IYE22_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29668..29862)
                     /locus_tag="IYE22_000774"
     CDS             complement(29668..29862)
                     /locus_tag="IYE22_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506006.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(30367..30552)
                     /locus_tag="IYE22_000775"
     CDS             complement(30367..30552)
                     /locus_tag="IYE22_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506007.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(31402..31743)
                     /locus_tag="IYE22_000776"
     CDS             complement(31402..31743)
                     /locus_tag="IYE22_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ9506008.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(31743..31973)
                     /locus_tag="IYE22_000777"
     CDS             complement(31743..31973)
                     /locus_tag="IYE22_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ9506009.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            32260..32598
                     /locus_tag="IYE22_000778"
     CDS             32260..32598
                     /locus_tag="IYE22_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506010.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(32803..33042)
                     /locus_tag="IYE22_000779"
     CDS             complement(32803..33042)
                     /locus_tag="IYE22_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ9506011.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(33018..33365)
                     /locus_tag="IYE22_000780"
     CDS             complement(33018..33365)
                     /locus_tag="IYE22_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ9506012.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         9935 a         7103 c         5843 g        10709 t
ORIGIN      
        1 ctactttgaa atataaaggt ttatccacta cttgattcag ttgggatcgc ctcctttttt
       61 tcattataag tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt
      121 ttatgttcta tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag
      181 gagcgatcga tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga
      241 agaatggcaa aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga
      301 tttagttgac gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc
      361 agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga
      421 ttatgaccga agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga
      481 atcattgcag tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt
      541 accattgcca tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt
      601 ctttgcttat ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc
      661 caacggtgca caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc
      721 cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt
      781 gattgctcct catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc
      841 gcaatctttt gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac
      901 gggtttgatt gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca
      961 tttgaatgac atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa
     1021 aacagtacgt tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta
     1081 ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct
     1141 tgcctctgga caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga
     1201 agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg
     1261 tctagaagaa aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa
     1321 taacttaggt atccaagtag gctaccccga taagatccca gcattattcg accagttcaa
     1381 aacagtacct gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact
     1441 cacattaaaa gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat
     1501 gagtgctgac acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc
     1561 agcgatcctt caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg
     1621 aatcggagct gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt
     1681 tgacgaatat ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa
     1741 caaagcacaa gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa
     1801 cggaaaatta accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga
     1861 agcagcaaaa acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat
     1921 ttggcgtaca aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc
     1981 ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt
     2041 tggtatcacg gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta
     2101 agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt
     2161 ctcaaatttt tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt
     2221 attagaggtt gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg
     2281 ctttccacat ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt
     2341 tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat
     2401 agttcctcat attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt
     2461 ttagtggaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa
     2521 gcagcaagga gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca
     2581 tttccttcta tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca
     2641 cttatttttt tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag
     2701 ctaatgctag caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt
     2761 tttcactttc ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg
     2821 gtgattcatt tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag
     2881 tttctcgcac agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt
     2941 ccccatcaaa tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg
     3001 ctgaaaaaat agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga
     3061 tcttttctgg gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag
     3121 ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca
     3181 caccatttgc ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg
     3241 gttcttgtac gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa
     3301 tagcacctac agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg
     3361 gtttaatctg catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct
     3421 gctgaccttc tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc
     3481 caagagtatt ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga
     3541 tttgatcttc gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc
     3601 ggaaagataa gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag
     3661 ccacattata tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc
     3721 tattggaatc gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag
     3781 ggcccgttaa agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc
     3841 ctggtaccca gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac
     3901 ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac
     3961 cgtcaaatac atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa
     4021 atcctggatc ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg
     4081 cttcgaaagg atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag
     4141 catgaatctc ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg
     4201 cactatttgt aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat
     4261 ttccagttcc tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca
     4321 actgaatcgg gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga
     4381 aggaataagt tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt
     4441 gtgtcagaga taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta
     4501 cataaactgt cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt
     4561 cataactagt catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga
     4621 aagtaaattc gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag
     4681 tgatagccaa gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca
     4741 ataatccgtc agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac
     4801 tagaggtcac agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat
     4861 ctgtcgcagg atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg
     4921 ctgcagcaaa tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat
     4981 acatgatgtc agaagccatt tcttctcctg tttcaggatc gtaataatat gcctctaaac
     5041 cagtattcgt ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca
     5101 gattcccatg accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg
     5161 ctgaagtctc taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg
     5221 gatcttcaga tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt
     5281 tgctgccgct cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac
     5341 tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta
     5401 ctgcgtctct taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta
     5461 tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg
     5521 tcattccatc tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca
     5581 cgccgtcttc taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa
     5641 cccctttcca ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt
     5701 ctttcatcat aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtattt
     5761 cctccattgt tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt
     5821 gttcgttagc agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat
     5881 tcatcattag cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa
     5941 attagaaagc gtttacttaa agtattacct aatttatttt tactgtcaat aaaaaacaaa
     6001 attataataa gtgtatttta taaaaagaaa aaatataact taagttgttg ataataaaga
     6061 gatttattaa cgtaaaaaga ataagttttt ttaaaataac gtataaaaaa gagcataaaa
     6121 caaagtcgtt tgttttatgc tcttctttta ttattttgtg ctttttattt tatcaaatca
     6181 ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt
     6241 catgtccaac gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta
     6301 gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat
     6361 aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg
     6421 aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac
     6481 tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata
     6541 agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa
     6601 aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc
     6661 ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg
     6721 agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat
     6781 ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg
     6841 atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt
     6901 ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat
     6961 ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt
     7021 tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc
     7081 ttcataatat agaggcattc ctggtaattg tcctgggttg tagccaaatc ctgttgcatc
     7141 tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc
     7201 gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag
     7261 acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg
     7321 gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct
     7381 taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat
     7441 ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag
     7501 gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc
     7561 gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc
     7621 aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc
     7681 gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc
     7741 cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat
     7801 cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag
     7861 atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac
     7921 cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga
     7981 agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc
     8041 tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat
     8101 cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa
     8161 agcaatgatc ccgacaccgc caggatgaaa taccagttct ctcttcgaag ttcctccatc
     8221 tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg
     8281 actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt
     8341 aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc
     8401 atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt
     8461 atagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca
     8521 acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat
     8581 tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat
     8641 tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac
     8701 aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga
     8761 cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc
     8821 aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc
     8881 cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg
     8941 aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata
     9001 atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg
     9061 cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg
     9121 aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata
     9181 ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca
     9241 ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa
     9301 tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag
     9361 atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa
     9421 cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag caacagacag
     9481 ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag
     9541 caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa
     9601 aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg
     9661 taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc
     9721 gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg
     9781 aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag
     9841 ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa
     9901 acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag
     9961 aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga
    10021 tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc
    10081 atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc
    10141 aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc
    10201 tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg
    10261 ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga
    10321 aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc
    10381 ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag
    10441 aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga
    10501 aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc
    10561 ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc
    10621 cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat
    10681 cagtccaata accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat
    10741 caatacaata caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa
    10801 ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag
    10861 catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa
    10921 tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg
    10981 cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag
    11041 taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc
    11101 tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg
    11161 gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat
    11221 gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg
    11281 gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac
    11341 aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc
    11401 aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata
    11461 taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac
    11521 gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc
    11581 gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc
    11641 agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc
    11701 tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat
    11761 cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc
    11821 agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt
    11881 caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt
    11941 ttttagttct tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa
    12001 atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata
    12061 ccctaaaagg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
    12121 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
    12181 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
    12241 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
    12301 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
    12361 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
    12421 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt
    12481 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
    12541 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
    12601 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
    12661 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
    12721 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
    12781 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
    12841 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
    12901 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
    12961 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
    13021 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
    13081 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
    13141 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
    13201 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
    13261 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
    13321 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
    13381 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
    13441 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
    13501 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
    13561 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
    13621 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
    13681 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
    13741 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
    13801 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
    13861 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
    13921 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
    13981 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
    14041 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
    14101 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
    14161 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
    14221 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
    14281 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
    14341 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
    14401 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
    14461 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
    14521 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
    14581 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
    14641 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
    14701 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
    14761 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
    14821 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
    14881 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
    14941 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
    15001 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
    15061 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
    15121 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
    15181 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
    15241 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
    15301 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
    15361 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
    15421 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
    15481 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
    15541 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
    15601 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
    15661 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
    15721 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
    15781 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
    15841 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
    15901 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
    15961 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
    16021 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
    16081 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
    16141 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
    16201 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    16261 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    16321 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    16381 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    16441 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    16501 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    16561 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    16621 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    16681 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    16741 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    16801 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    16861 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    16921 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    16981 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    17041 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    17101 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    17161 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    17221 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    17281 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    17341 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    17401 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    17461 cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga
    17521 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    17581 ttttttatta ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt
    17641 cttgcttcat tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga
    17701 ccactcggct ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt
    17761 gttgcggatt cataggcaga gactggaact ttcccggatt ctaattcagc catctcctgt
    17821 ccgttaacta ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg
    17881 acctcgattc tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca
    17941 aaataatagg tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc
    18001 gaaatgaatt tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat
    18061 tgatttttca agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc
    18121 tgatatagag caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga
    18181 cgtccatact tgaagaagta tggatctctt agataaccga catttttagg atagatatga
    18241 atctcttgca gttcctcggt tgattctgtc gggtgataaa ttggcaaaat agcagcaatt
    18301 gggatagctg tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg
    18361 aaaatcaaat aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc
    18421 ccgtctccta atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca
    18481 agcctttctt ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg
    18541 acgaattctt taggagtcat gttttcagca gctttttttt catgataata atcggtcatg
    18601 tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt
    18661 tcccctgctt cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta
    18721 taatcgatat ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat
    18781 tcttctgccg acatcgcatg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat
    18841 tgaagcatca aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga
    18901 caaacaaacc ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga
    18961 ataatcgtcc ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa
    19021 gctgtaatct gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag
    19081 gcgtctctgc ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt
    19141 ttcctggaac tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa
    19201 taaagtaacc tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga
    19261 ccccttccaa caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc
    19321 ctccctcgat gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc
    19381 cttcaagacg tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta
    19441 gttcatcttc taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg
    19501 gagcagtttc gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg
    19561 caaactggaa cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct
    19621 catattcacc aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta
    19681 ctcctacggc acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt
    19741 ctaacggatt accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga
    19801 tatcgaaacc tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct
    19861 cccgataatc aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg
    19921 ttgtatacgt gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta
    19981 tagcattatc gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt
    20041 ctgggttctg gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct
    20101 tataaacaaa tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta
    20161 catatttccc gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt
    20221 cgttcacacc agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg
    20281 tttcataaag ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat
    20341 tgatattcat gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat
    20401 catactctgt gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt
    20461 cataatagga attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt
    20521 catctacaag ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt
    20581 ggaattgata cgtctcatct actaactgat aacgtggtgg tccagcgatc tctctgatcc
    20641 aaaatatgtc gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga
    20701 tcgctattcc gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg
    20761 gattttggtc atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct
    20821 tgctgacttg gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca
    20881 tccgtccagt agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt
    20941 tcactgtgca aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc
    21001 tgaccatttc cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc
    21061 cgacatacgt tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat
    21121 cccatatttc tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact
    21181 gaatatctgc cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa
    21241 gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa
    21301 tgtcattctt gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag
    21361 caatattttt caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc
    21421 catcgattcc taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca
    21481 gccaatgatg ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc
    21541 cattcgtatt gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt
    21601 cttttacctc tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag
    21661 atgcataggt aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa
    21721 aatttctgaa caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc
    21781 tccaagctcc attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg
    21841 gtgtcccatc tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt
    21901 ctttatacaa tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg
    21961 gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg
    22021 gaaagatgtc cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat
    22081 tcaccatacc gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt
    22141 ttactgcctc aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac
    22201 tcatactcgc tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct
    22261 cgcccccgat gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat
    22321 cagatgctgt gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat
    22381 aatccaagta actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg
    22441 gaacgtttct atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc
    22501 gccagttcgc tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg
    22561 gcgagccttt gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg
    22621 tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc gttttcaact tctccgctag
    22681 gatagatata ttcatagggg tcttgcgtag ctgttggccc aagacgctcc aaaataccgt
    22741 tgaataactg gcgttgaatg cgctggagtg cttgaccgct gggtacgatt ggttcgtcca
    22801 gcttttccat ctcattttct tcgctacgtt gaatttcagc ggaatcttct gtatcagttt
    22861 cattttcttg taattcttgg ttatctattc tttcatcaac caccacttga agaggtagtt
    22921 acccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt
    22981 cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt
    23041 cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa
    23101 tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt
    23161 tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt
    23221 ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta
    23281 catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag
    23341 ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat
    23401 atttgtcttc tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa
    23461 ttttaatttt aaatcaaatg atatcctttt tagttaaaaa acaccaatta tacaacattt
    23521 attattaaaa ttaaattata aaattttata acgttctcat ttaacaaaac ttaagttatt
    23581 aagacaaatg aataaacaac tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc
    23641 atttacgcac tcatgcttta ttcgtacaat taattcagat aacggcagcg cataggatgc
    23701 caataacact tcctaaaata gctcctccta aaatgtcagt aggataatgt aaaagtaaaa
    23761 aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc taatattcct gcaactggac
    23821 ttaattcgaa caacgcagct actgataaaa aggctagcgc tgtatgtcct gaaggaaaag
    23881 aagaagtctt cggtttactt gtgtaaagga caagccgacg atttttttca taaggtcttt
    23941 tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa aaaatagatc aaactgccga
    24001 taagaatggc tagaccatat tctctagtat caggtatcaa gactaaaaca gcagcaagga
    24061 gcaaccaaat aaaaccacca ttagagatat tactgataaa tttcaaaaaa ttgatttttc
    24121 gactatccgt tttccgttgc ttggcgaact tgttgataat actatcatcg attttagcaa
    24181 tcaaacgcaa catgttattc ctccttttta agaacctttt cttgcgtacg atggataaaa
    24241 agcaggactt gaaaataaaa caagaaactg aaacctaata aagcaaaata gattggtcct
    24301 gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg
    24361 aaatcattaa aaaacaagaa aaatgcagta atccaagaag cttctttttg ttccagttga
    24421 agcaagattt tgtaactaaa gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac
    24481 atcaatgtat agccaattcc cccaccccaa tagcggaaaa tctgataatc gatcaataga
    24541 agaagaaaat aaacacttgc taaactatct tgctttccat aaagagagaa gttctcacga
    24601 aactttttcc agaatagtga accgagcttt ttctcttcat tttttttgta atgataaatc
    24661 atatacagaa ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac
    24721 catagtagct gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca
    24781 ttggcttttt taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc
    24841 cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg
    24901 ttgctcctgc catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc
    24961 caatctgtaa cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat
    25021 tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa
    25081 gcatcaaatg actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca
    25141 attcattatt gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga
    25201 agaacgatgg aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata
    25261 catattgagg gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa
    25321 acatcagctt tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg
    25381 aaaggacatt accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc
    25441 aaaaaggaaa ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa
    25501 aggtcggagg ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa
    25561 gcaatggaat gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt
    25621 ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt cacctccaga
    25681 ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag
    25741 aaacaggatc attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt
    25801 ccacatatgc agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt
    25861 catgacataa gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta
    25921 aaaaataata ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc
    25981 tccatcgatt tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta
    26041 aagtagcatt gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca
    26101 ttgtggatca aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat
    26161 caatcctgca gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa
    26221 gactgccggt aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag
    26281 tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata
    26341 aacggtgttg tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt
    26401 tgctagacca atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac
    26461 tgaaaaaagc ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa
    26521 taaataggcc catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg
    26581 cctcctctgt acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta
    26641 ctgctttttg tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt
    26701 caatctgttc attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca
    26761 ccgtattttt aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt
    26821 tgtcattatt cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt
    26881 cgccgaattt ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct
    26941 caggatcttt tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc
    27001 ctgaaccttc ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat
    27061 tttccatcaa actagagtaa taattgatat tttgggcacg catggctact aatccggaaa
    27121 taaatggatc agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc
    27181 cataataatc ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa
    27241 tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata
    27301 catcagcccc taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt
    27361 ttttatctag tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt
    27421 cactgattcc agcttcttta aataactttt tattataaaa aatgacttgt gtatctgtat
    27481 tgaatgggat cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa
    27541 aatccttaga agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg
    27601 ctctttgggc aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta
    27661 ttgcagataa agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga
    27721 tctcgttttg tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac
    27781 cggatcccca aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag
    27841 aatttgctgc acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa
    27901 acaatgctga acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa
    27961 ttttaatgta accgatgccg gttttaatat aacgatatca ttttgttctg aaaataacaa
    28021 tttataaggt atgattctaa aaaaattatt aatgatacca atactttata taattcgcga
    28081 gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt
    28141 aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg
    28201 tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat
    28261 gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa
    28321 agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat
    28381 ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga
    28441 tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt
    28501 aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag
    28561 tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca
    28621 agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct
    28681 ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga
    28741 aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat
    28801 tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag
    28861 tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg
    28921 cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa
    28981 ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg
    29041 actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct
    29101 caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg
    29161 cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag
    29221 gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt
    29281 gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca
    29341 cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc
    29401 tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca
    29461 atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc
    29521 gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat
    29581 aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat
    29641 gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc
    29701 ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat ctttatttat
    29761 tagatattca acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa
    29821 ataaggatgc tttttataga atagaactag tgttgttttc atagactctc ctttcttcat
    29881 atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg
    29941 aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa
    30001 atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct
    30061 tctatctccc atccagtata cataaccttg atacttcgac cacttatata tcgaacacac
    30121 aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca
    30181 gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca
    30241 tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa
    30301 atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat
    30361 tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact
    30421 tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt
    30481 tagcagccca accaacatat accctataaa agctaataag atggttagga atcctttaga
    30541 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
    30601 aataatattg acaaaaagtg actcaaaata aaaaaaacct tacattatac tggaatgaac
    30661 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
    30721 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
    30781 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
    30841 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
    30901 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
    30961 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
    31021 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
    31081 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
    31141 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
    31201 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
    31261 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
    31321 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
    31381 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
    31441 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
    31501 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
    31561 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
    31621 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
    31681 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
    31741 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
    31801 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
    31861 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
    31921 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
    31981 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
    32041 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
    32101 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
    32161 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
    32221 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
    32281 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
    32341 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
    32401 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
    32461 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
    32521 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
    32581 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
    32641 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
    32701 tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
    32761 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
    32821 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
    32881 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
    32941 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
    33001 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
    33061 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
    33121 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
    33181 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
    33241 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
    33301 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
    33361 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
    33421 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
    33481 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
    33541 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct
//
LOCUS       DACIRY010000020        33334 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.020, whole genome shotgun sequence.
ACCESSION   DACIRY010000020 DACIRY010000000
VERSION     DACIRY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.020"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(130..825)
                     /locus_tag="IYE22_000781"
     CDS             complement(130..825)
                     /locus_tag="IYE22_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ9506013.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IYE22_000782"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IYE22_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ9506014.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IYE22_000783"
     CDS             complement(2024..2215)
                     /locus_tag="IYE22_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ9506015.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IYE22_000784"
     CDS             complement(2236..3126)
                     /locus_tag="IYE22_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ9506016.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IYE22_000785"
     CDS             complement(3113..3880)
                     /locus_tag="IYE22_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ9506017.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IYE22_000786"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IYE22_000786"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ9506018.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IYE22_000787"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IYE22_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ9506019.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IYE22_000788"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IYE22_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ9506020.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IYE22_000789"
     CDS             8496..8834
                     /locus_tag="IYE22_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506021.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IYE22_000790"
     CDS             complement(9002..9760)
                     /locus_tag="IYE22_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ9506022.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IYE22_000791"
     CDS             complement(9778..10596)
                     /locus_tag="IYE22_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ9506023.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IYE22_000792"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IYE22_000792"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ9506024.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IYE22_000793"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IYE22_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ9506025.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="IYE22_000794"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="IYE22_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ9506026.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="IYE22_000795"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="IYE22_000795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ9506027.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="IYE22_000796"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="IYE22_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ9506028.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="IYE22_000797"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="IYE22_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ9506029.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="IYE22_000798"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="IYE22_000798"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ9506030.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="IYE22_000799"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="IYE22_000799"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ9506031.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="IYE22_000800"
     CDS             20638..20937
                     /locus_tag="IYE22_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ9506032.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="IYE22_000801"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="IYE22_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ9506033.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="IYE22_000802"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="IYE22_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ9506034.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="IYE22_000803"
     CDS             21904..23877
                     /locus_tag="IYE22_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAQ9506035.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFTAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="IYE22_000804"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="IYE22_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ9506036.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="IYE22_000805"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="IYE22_000805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ9506037.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            complement(26629..28095)
                     /locus_tag="IYE22_000806"
     CDS             complement(26629..28095)
                     /locus_tag="IYE22_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9506038.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(28405..30486)
                     /gene="cadA"
                     /locus_tag="IYE22_000807"
     CDS             complement(28405..30486)
                     /gene="cadA"
                     /locus_tag="IYE22_000807"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ9506039.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(30628..30807)
                     /locus_tag="IYE22_000808"
                     /pseudo
     CDS             complement(30628..30807)
                     /locus_tag="IYE22_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(31010..31369)
                     /locus_tag="IYE22_000809"
     CDS             complement(31010..31369)
                     /locus_tag="IYE22_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ9506040.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(31399..31740)
                     /locus_tag="IYE22_000810"
     CDS             complement(31399..31740)
                     /locus_tag="IYE22_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506041.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(31737..32411)
                     /locus_tag="IYE22_000811"
     CDS             complement(31737..32411)
                     /locus_tag="IYE22_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ9506042.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
BASE COUNT        10039 a         6685 c         6359 g        10251 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag accaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgttaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatt aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggcgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 tcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagact
    18181 atgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ctgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa
    19021 gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc acacatttta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaagcaaa agcgacgatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa
    19681 taccaattat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggttaga ggaatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaagatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 agccatcaag actaattgtg taagctagca agaatgaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatc agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattggat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca acgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttactgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aattaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aagggttcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    26101 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    26161 caaacgtcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
    26221 gctgatctta cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag
    26281 tcacaatatc atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt
    26341 aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat
    26401 acgcccaatc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
    26461 tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa
    26521 aacaagccga aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct
    26581 tgaagctgac cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac
    26641 cactgtttct ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat
    26701 catacctaat aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca
    26761 tgctttagga tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc
    26821 agtgaaggca aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt
    26881 atattcagaa ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca
    26941 taggtaacgc attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt
    27001 catatctttg ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag
    27061 aagataacca ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa
    27121 ttctggatcc gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc
    27181 ggagatttgt ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc
    27241 atagtaatta cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg
    27301 aatattatcg ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc
    27361 taccattgct gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga
    27421 gttgacataa ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt
    27481 tgtgaaataa ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt
    27541 ggcaaattcc atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat
    27601 cgacatgacg aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc
    27661 ttttgttgat aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg
    27721 tactgacatc gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag
    27781 gattgcttga ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc
    27841 atagtatgcc agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt
    27901 actatccttg aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat
    27961 ggccaaaatc aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac
    28021 tacattcccc atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa
    28081 ttgtttgttg ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca
    28141 tattcaattt ttaacaaact tacaagacag tgttatttta tgtgtgtatt cttttattta
    28201 atacacatcg ttttttcata ttttttccat caaaaaaata agttattttc tcttatttca
    28261 tttaccttat tgaatttcaa gctattttga attttctgca aaattaaaaa cattagttat
    28321 tttataatcc tatctatttt ctttacattt ttcattaaga aaaggatata aaaaaagagg
    28381 acgttcattc gtcctcttca ttgatcaacg tttatttttt acatgcaagc ttcgcatggc
    28441 attcaaaaca gctaatactg tcaccccaac atctgcaaat actgcatctc ccattgatgc
    28501 aaaaccaaaa gcacctaagg caagtaccaa aatttttatc ccaatagcaa agacaatgtt
    28561 ctgtttgacg attttcaatg ttttctttgc taaatggatt gcttctgcaa tcttcgatgg
    28621 ttgatcattc atgatgacta catctgctgc ttcaattgca gcatcgcttc ccaatccacc
    28681 cattgcgatt cctacatctg ctcttgctag caccggtgca tcgttgattc catctccgac
    28741 aaatgcggtc tttttattgt ttctttgtaa gatttcctct aaccgctgca ctttatcttc
    28801 tggaagcaat tcgctatata tcttgtccac gcctacttgc ttcccgatat gatcagcaat
    28861 ttttttcgaa tcgcccgtca acatgactgt gttcttcaca ccagcctttt ttaattgaac
    28921 caatgcgtct acggcatctt ctttcaaggt atctgcaata acaagataac cactgtaact
    28981 ttgatcgatc gctagataaa gcaatgtacc gatttcttga cttgaaggaa aagagatttc
    29041 aaatctttcc atcaatttgt gattacctgc taatacttgt ttgccatcaa cttcagctaa
    29101 gatcccttca cctgcaaatt cttggatatt agctgttggt aaaagcggac ctgcgtagcc
    29161 ttctaaaaca gattgagcaa tcggatgtgt agaaaactgt tctgcactcg caacatattg
    29221 catgaatatc tttggatcga tttttgtatc gatcgtctgg atcgaaaagt cacctttcgt
    29281 taatgtgccg gttttatcga ataccactgt ctcagtttgt gcaagcaact ccaagtaatt
    29341 tgaacctttt actaatacac cgattttact tgctcctccg ataccgccaa agaaactaag
    29401 gggtacggag atcactaatg cacatggaca tgagatcact aaaaatgtca atgctcgata
    29461 gatccattca gaaaacgccg ctccagaaac aacaagtgga ggtaaaacag ctaataaaac
    29521 ggccaatcct acaacgactg gtgtgtaata gcgcgcgaat ttagtaatga aattttcagc
    29581 tggtgctttt ttactgctgg cattttctac taattctaat aatttgctga tagtagaatt
    29641 ttcataagat ttttctactt gaactaaaaa tggtttattt ttattgataa atccacttaa
    29701 tactgtttca ccaacatata cagatttagg gactgattct cctgtaagtg ctgacgtatc
    29761 gactaatgat tctccttcga tgatcgttcc atctaacggt acacgttcac caggcttcac
    29821 aagtacaaac tgaccgatct gcacgtcttc agggttcacg gtcatcaatc cttccgtcgt
    29881 ctgcacgtga gctgtttctg gacggatcgc catcaattcg cgaatcgatt ttcttgaatg
    29941 actgactgcc aatccttgga aatactcgcc gatttgataa aatagcatca ctgctacggc
    30001 ttctggatat tcaccgatca acatagcgcc aatcgtagca acagacataa gaaaattctc
    30061 atcaaagatt tttcctttga cgatattcat cactgccttc ttcactacat catatcctat
    30121 cagcagataa atcaataaat accctatcaa gcgaattggt tcattaaaat caaataatag
    30181 caataaagcg atacctgcaa gggaaaataa aatccggaac agcgtatttc tctctttttc
    30241 atttccatgt tcagatactt tttccttatc tatttctgtt actttgacat ctggttctaa
    30301 ttccttcact acttttttta tttcttgttc aatcgtagcc aaatgatctt cctctgcgtc
    30361 aatcgttagt tttccagaag tgaagtttac cgttgcttcc ttgactccat tgatcttctg
    30421 aactcctttt tcgattttca ttgcacagtt cgcacaatct aaaccttcta atctgtaact
    30481 tttcatttta atgacctcct cgcctcaaca tatgaatatc tcttcataac taattataaa
    30541 tgaacggtta ttcatatgtc aaggtatccg aaagaaaaaa tgaaaaacat ctgcatcgga
    30601 aaatttcttc catacagatg ttttggctta ttctttcttt ttcttttcga tcattgccac
    30661 tgctcttatc caacccgcac ttgccttctt gatcttgaca ggatagcaac taagtttgaa
    30721 gcctgttgct ggcaatttgt ctaaattcat taatttttcg ataggacaat atcttttttc
    30781 aatccctgca aaatgtcctt cccagatatt cgaagctcca ctacgctttc aaaaatatag
    30841 aaagagttct ttaatgaaaa ttatttcaac tttccataaa cagtaacccc atataaataa
    30901 aagacgtaaa agtcacatgg aagaacttat acaggagaac gcatagagtc aggtgaaata
    30961 catgtatccc acctgactct atgctttatc taacctttat tattctcttt tatacaattt
    31021 ctgcaccgaa tggcatcaac gccaatcgag caagtttgaa agattgcttg gcaaaaggta
    31081 tccctatgat cgtgatacat agaaggatgc cgctgacgac atgtgcgaga gcaattggta
    31141 ggcctgaaac gatcagccag atgatattaa caatcaatga catgccagac tggtgataaa
    31201 ttacttcttt gccaaaaggc gcaaaagaaa gggaggcgat cttaaaacat tgcataccga
    31261 ttgggatacc tatgatcgtg atacaccaaa gtatccccgc tgctaaccag ctaagaccac
    31321 cgattagtcc gccgaatata aaccagatga catttcctaa acaactcatt gtatttctcc
    31381 ttctattact gtttttgatt aaaactgggc aaaaaatgat tttattcttc tttttttagc
    31441 ttccagtatc aaatattctg caaatttttt ttggatttct tccgtatttt cttttgttac
    31501 taatgtgtgt tggattagtt ttcctgatgg tcgctgatcc aatcgttctt ctgactgttg
    31561 cacgtccgct tgcgcaaatc cgataatccg tttttctttt gttacagcaa cgagcaatag
    31621 tttattctta tcatttaggt cagctgccgt ttttgctttt acgaatgatt ctggatgatc
    31681 agtaatcagc tgattgattt gaggtagatc ttcttttact gcccaccgta tttccatcat
    31741 ttctcacttt tctttcgctc atttttatct ccactaaaaa aatcgactgc ggcaattgca
    31801 tctatccagc tttgtgcaat gatctcatgt ccgccaatcg ttgggtggac cccatcttct
    31861 cttgtatatt tcgaaaaacc ataaacaatc cctaaagcat tgaatatacc atctaaagga
    31921 accagcaatg cgccatattc cttagcaagt ttacgaacaa tttggatttt agggtctaag
    31981 tctttacgcc agccatgacg gtcgtcggtt ataggaagta caaacggctc catcaagatg
    32041 atgttgcaga ttcccactgt ttgaagttca tttagtaatt ttcgataaac ggattcaaat
    32101 tgctgagcag atttctcagt tccgaatata gcctgatcgc tagtattatg ccaggtatca
    32161 ttgattccga tcaaaatcga tacatagtca ggttgtaaag ctaaacaatc ttcttgccag
    32221 cgtacttgca gatctgcaat tttgtctcct cctatcccac ggttcatgca ttcaaattcc
    32281 atcgttggat attgggagat tagttttccg gcaatcaaaa gtggataacc tttacctaga
    32341 tcagttgctt ctgctcggtc tcttcctgcg tctgtaatgc tatcaccgat aaataatata
    32401 cgttttttca tttgtcaaaa actccttttt gaataaaacg atgattttct tccttatatt
    32461 atcccataag ccctatcaag acgaaagaga aaatatatgt ataaaaaatg acaccagcct
    32521 agtcccttca aacaattgaa tgaacgctca tcaagtgtct agttttttat gctatttttt
    32581 tcttaacgtg ccggattccc cttgattcct tcgttgtttg gtccagagag atcgatcaca
    32641 cctaagcttc cgtcaatcag accactgatg acgccatcag aacatatatt tataggctca
    32701 tctaagactt ctagtaaatg tccctttact aatatctctt ctagattgct tgggatagat
    32761 tggtcataac tttatctggt ccgatgattt caagggtgaa ttgaacttac atatggatta
    32821 ttgcatgctg aaaatacgga cctactccat cttcgaatgt tactattagt ttgttttcag
    32881 gtgctagatg ctgctttatt tcaacagcag aagcatcatc aaatcagatt tccatttgaa
    32941 ttaccttctt tgtaaatgtt tgattgattg cgatttcgtt atagaacctt gtgaatcaaa
    33001 tagcaatttc ttttgctttc ttctcttgtc tgctggaaac ttcctttatc ccgtatttta
    33061 aaaaaagaac tgcgagagtt cgcagttctt ttttagaata aacggtgctc tccggccaac
    33121 tcctcggtca caagtcacaa tagtcatcaa acatgaaaaa ctgttttttg aaattactcc
    33181 ttgtgactcg gagctgaacg gcaggcgcac aacctcttga tataaacggt gttcaatcag
    33241 ctgaccctcg atattagcta aaaaaacggt aaaatatgag aagctatttt tccgttttgt
    33301 ttctctaata ctcaggtcaa ggcactgatt tcac
//
LOCUS       DACIRY010000021        32151 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.021, whole genome shotgun sequence.
ACCESSION   DACIRY010000021 DACIRY010000000
VERSION     DACIRY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.021"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            206..1687
                     /locus_tag="IYE22_000812"
     CDS             206..1687
                     /locus_tag="IYE22_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ9506043.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(1790..2497)
                     /locus_tag="IYE22_000813"
     CDS             complement(1790..2497)
                     /locus_tag="IYE22_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ9506044.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(2576..2914)
                     /locus_tag="IYE22_000814"
     CDS             complement(2576..2914)
                     /locus_tag="IYE22_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ9506045.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(3025..3987)
                     /locus_tag="IYE22_000815"
     CDS             complement(3025..3987)
                     /locus_tag="IYE22_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ9506046.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(4002..5333)
                     /locus_tag="IYE22_000816"
     CDS             complement(4002..5333)
                     /locus_tag="IYE22_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ9506047.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(5341..6021)
                     /locus_tag="IYE22_000817"
     CDS             complement(5341..6021)
                     /locus_tag="IYE22_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ9506048.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(6240..6719)
                     /locus_tag="IYE22_000818"
     CDS             complement(6240..6719)
                     /locus_tag="IYE22_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ9506049.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(6846..8525)
                     /locus_tag="IYE22_000819"
     CDS             complement(6846..8525)
                     /locus_tag="IYE22_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ9506050.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9073..10575
                     /locus_tag="IYE22_000820"
     CDS             9073..10575
                     /locus_tag="IYE22_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506051.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            10588..11250
                     /locus_tag="IYE22_000821"
     CDS             10588..11250
                     /locus_tag="IYE22_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506052.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(11351..11554)
                     /locus_tag="IYE22_000822"
     CDS             complement(11351..11554)
                     /locus_tag="IYE22_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ9506053.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(11665..12849)
                     /gene="iadA"
                     /locus_tag="IYE22_000823"
     CDS             complement(11665..12849)
                     /gene="iadA"
                     /locus_tag="IYE22_000823"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ9506054.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(13006..14745)
                     /gene="yfcC"
                     /locus_tag="IYE22_000824"
     CDS             complement(13006..14745)
                     /gene="yfcC"
                     /locus_tag="IYE22_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ9506055.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(15517..16131)
                     /locus_tag="IYE22_000825"
     CDS             complement(15517..16131)
                     /locus_tag="IYE22_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9506056.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(16737..17114)
                     /locus_tag="IYE22_000826"
     CDS             complement(16737..17114)
                     /locus_tag="IYE22_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506057.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(17414..18295)
                     /gene="galU"
                     /locus_tag="IYE22_000827"
     CDS             complement(17414..18295)
                     /gene="galU"
                     /locus_tag="IYE22_000827"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ9506058.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(18338..19357)
                     /locus_tag="IYE22_000828"
     CDS             complement(18338..19357)
                     /locus_tag="IYE22_000828"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ9506059.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(19990..20994)
                     /locus_tag="IYE22_000829"
     CDS             complement(19990..20994)
                     /locus_tag="IYE22_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9506060.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(21007..22056)
                     /locus_tag="IYE22_000830"
     CDS             complement(21007..22056)
                     /locus_tag="IYE22_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9506061.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(22073..22894)
                     /locus_tag="IYE22_000831"
     CDS             complement(22073..22894)
                     /locus_tag="IYE22_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9506062.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(22881..23660)
                     /locus_tag="IYE22_000832"
     CDS             complement(22881..23660)
                     /locus_tag="IYE22_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9506063.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(23677..24147)
                     /locus_tag="IYE22_000833"
     CDS             complement(23677..24147)
                     /locus_tag="IYE22_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9506064.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(24159..24578)
                     /locus_tag="IYE22_000834"
     CDS             complement(24159..24578)
                     /locus_tag="IYE22_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ9506065.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(24659..27427)
                     /locus_tag="IYE22_000835"
     CDS             complement(24659..27427)
                     /locus_tag="IYE22_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9506066.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27627..27851
                     /locus_tag="IYE22_000836"
     CDS             27627..27851
                     /locus_tag="IYE22_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506067.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            28630..30345
                     /locus_tag="IYE22_000837"
     CDS             28630..30345
                     /locus_tag="IYE22_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506068.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            30348..32111
                     /locus_tag="IYE22_000838"
     CDS             30348..32111
                     /locus_tag="IYE22_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506069.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         9528 a         6707 c         5469 g        10447 t
ORIGIN      
        1 atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg aaataaacaa
       61 tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa ggcgacactc
      121 tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta actatataca
      181 aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag agttacttgt
      241 tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca atgacttaca
      301 tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac tcaagcaatt
      361 tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa ccaaagaaac
      421 cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc taaaagaatc
      481 caatcgtttt aactatttga tgttactaat caattatcca caaactgctg taccaaaaaa
      541 atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga caaaacagct
      601 caatcattcc ttaaaaaaat tccatttacg gatcctatct gtaaaaaatt gctgtactct
      661 tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag atacatttgg
      721 aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga acagtacaga
      781 tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt taactttatt
      841 catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa tcactgaatt
      901 gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt tttcagtgag
      961 tcgcccttgt gaagagacag aaacagagag agcctacttt aattattttt tctattgttt
     1021 ttttccaaat caaatctctt ttcgacaaaa acaggagatt ggttttttat tttccacaag
     1081 caaaaacctt atttgtcgac aagtcggtca attgatgact tacttaactc aacagtttcc
     1141 tctcaaccat ttagaaaata aaataccaat ttatttttat tttcttgtgt tggcagttgt
     1201 ttttttcgaa ctgttgggtg aatccagcag tagtttttct acactttgct ttccagaagc
     1261 cacgtccaat cattctagtt cttttataca ggaaaagaaa ttaagagaaa aaatcgatac
     1321 aattttttca aaaaatgggg aaagaaacca aatcagtcaa taccttagtc aattgatcca
     1381 acacttacta caaacagaaa cgaaagagca gttactcatc catttgcaga taaccaaaca
     1441 gtttacagga aattatcatc ttcaaagcca tttgcagcag ttatttcgca aagatgctgt
     1501 taaaattacc gcatattcag aacatgctga tttgatcatt acagatcagc atgagaaaat
     1561 ttcagatgaa acggagactt tctatctgga tacaacgcac aatacagtgt tatttggcaa
     1621 gttaataaaa gcggttcatg aactgtatat gaaaaaaggt tatgagaaca aaacaaagag
     1681 gatgtgacaa aattcttgtc acatcctctt attccaaata aatggtattc aatcagctaa
     1741 ctctcggtat tagctcagat caaagcgctg atttcacaac ttctttattt taatctcttc
     1801 ggttgccgcc gaacaataag atcaagcgca acagctgcag gaatgtcgaa agcgctgctg
     1861 caacataagt cagtgctgct gcaaataata cgtgtcttgc catcggtact tcttcttccg
     1921 tcagtagtcc gccttcactc aaaatttgga ttgctcgccg agacgcgtta aattcgactg
     1981 gtaatgtcac caattggaaa agtaatgcta aagaaaaagc gaaaatcccg atattgatta
     2041 acgtagagtt ccagctaagc aatacaccta ccataatcaa ggggaacgaa atcatcgaac
     2101 caaagttagc tgctggaaca agtgctgcac gaatcttcaa tgggacataa cccgaagcat
     2161 cttgtaccgc atgtccgcat tcatgagctg ctacacctat cgcagcaact gaagtagact
     2221 gtgcagttgc ttgagacaaa ctcaacatct tattacctga attgtagtta tctgtgagat
     2281 ctccggcaat ttgttgaaca ccgacattat ttatctgttg actttgtaat atatattgtg
     2341 ctgcttgtgt accagtcaca ttgtttttgc ttctcacttg atcgtatttt cggaaagtac
     2401 tattgacgta cgctgacgca attccggaaa taactaatcc aagaatgacc aaaagaaatg
     2461 tcggatctaa aaaaccatac attccataac catacatttt cgtttcactc cttttcgttt
     2521 actttatttt atcattagta gtaggaaaag aatgtgaaaa gaccttgaat attctttaat
     2581 tctttttcac aaattccgat ttcagtttca tcggtccaaa tccttctacc ttgcaatcga
     2641 tattatgatc gccttctact aaacggatgt tcttgacttt tgttccttgt ttaatggcac
     2701 ctgacgcacc ttttactttc aagtctttaa tcactgtcac gctatcgccc tctaacagac
     2761 ggtttccatt tgcatctaat accactaatc cgtcttctat gacagtttct tgttcttctt
     2821 tgttccattc gttcccgcat tctgggcaaa tcaacaagcc tcgatcttca tatgtgtact
     2881 ctgagccaca ttctgggcaa tttggtaatg tcatcttctt tctcctttaa aatctttagt
     2941 gagcactatt gtaacggaaa aagaagatct tgaaaagata catttgtgat ttttactaca
     3001 aaaggcacgt tttcgaacga agtatcattc tgtttttctg ctcttatacg attggcatat
     3061 atcttggatc tctttataga tttcctctac ttctgctaaa tcttttgcat tcgcaccact
     3121 tgctaatgga tggcctcctc catgatggcg tttagccaat tcattgatga tcggcccctt
     3181 agaacgtaaa cgtacgcggt aataccctga aggctgctga acaaaaatcc cccaagccag
     3241 cacttcgtca atcactccag gaagtgaaac gatcgaagct gtctctgaat catcgattcc
     3301 atatttatca agaatttctt gcggaaggat tattcttcct gcaccatttt catccacctg
     3361 gatattttga taaagataac cggaaagttt agcgactttc atcggcattt gttcaatctc
     3421 tctgttcaaa gcacttgcat caaatggata atttctcaat atcgaagcaa tctctaatgt
     3481 acgggacgtc gtggagggat acaaaaagcg ccctgtatct ccgacgattc ctgcataaag
     3541 taaccgtgca gccttaacgt ttatctttaa agattcagga aacatcaaag caaattctgc
     3601 aatcatctca ctacagctac ttgcttttgt atttacccac acaagatctc cataagggtc
     3661 atcattggga tgatggtcga ttttgatcag tttttctgcc aactgatatc tttgatcact
     3721 gattctgggt gaattagctg tatcagtcac gatgactagc gctccttggt agaagacatc
     3781 ttcaacttct tgcatttctg ctaaaaagcg aagcccttca atcgtttcac ctacttgacg
     3841 tatttctttt tctgggaaac tttcccgtaa tagttcggct aatcctactt gtgatcctaa
     3901 tgcatcagga tcagggcgca aatgcctgtg gataatgatt ttatcgtatt tttttattgt
     3961 atctactatt tcttgctgta ctgtcatttc actcctgctt tctaagaacg ttccattact
     4021 tgacaaacaa cgattgcttt ggaaacgatg gtgttttcaa taaacacttc gatatccagt
     4081 ttagcggaac gtctgccgat ttccaataca cgtggacgaa tgtctaattc gctttccaat
     4141 tggatcagcc gcaaataatg taggttcact tgttcaatca agatatttcg tttttggttc
     4201 atcaccattg ttttttgtgc gacatcagaa atgatttcgc taagtacgcc aaaagaaatg
     4261 gttccaacgc cattgaccat ttgtggtgct actacgaatt tgaacttagg gttttccaaa
     4321 cgatttcctt cacggtcagt atcaatcgta tggatatttc cagatatctg atccgaaatc
     4381 gtatctgcaa tctgcggttg tcgctggaca agctgcatcg ctttcattac atcttgccgg
     4441 gtaatcaagc cttccaatgt caagtcatcg gaaacgaccg gcatgacttc tagcccatcc
     4501 cagatcattt gatggctaac agaggcaata ctcattgttt tctttacact gattggttct
     4561 ttcgtcatca cccgatcgat gatctggttt ggcgatttcc ctaaaacatc ttttgccgta
     4621 acaatcccca tcaaacgatg atggcggttg acgactggaa aacgcgaatg ctgcgtttct
     4681 tctgacaaac gctgataatc agaaatcgta tctgtctgct gcaaataatg agtcttttca
     4741 gctggcatat agatatcact gacaagcatg atatcttttt tgatcaattg gtcgcttaac
     4801 gctcggttga tcatggttgc gactgtaaat gtatcataag aggtgcgtaa gatcggcatt
     4861 tcaagatcat cagctagctt tgcaatctca gcagtcgtat tgaatcctcc tgtgatcaat
     4921 actgcggcgc catcttccaa tgctaatttt tggacttccg cccggttgcc gacgatcatt
     4981 agtgatcctg gcgtaatata gcgttccatg gcatgtttag tcatcgcgcc gatgacgaat
     5041 ttattcaaga ccttgtctaa tcctgccgcc ccgcctaaaa cgtccccttc aatgatcctg
     5101 acgacctcgc caaatgtcaa acgttcaata tgttttttta atttgcgttc gatacggatg
     5161 gtccctactc gttgaattgt tgaaactagt ccgacatttt ctgcatcttt gattgcacgg
     5221 tacgctgttc cttcactgac attcaattct tttgcgatgc ttcttacaga aattcgattg
     5281 ccaacaggta aactttcaat atattctaaa atcaaatcat gttttgtcgc cattttctgc
     5341 ctacacttcc agcgtttccg agacgctcat tatttttcct tcactatctt ccagtaactc
     5401 tacaaaagat tcgggatctt gttcaatgac agggaaagta ttgtaatgga ttggaactac
     5461 tttcttggca cgaattgttt gcgcagcctt tgctgcatct tctggcccca tcgtatagtt
     5521 atcgccaatc ggcaagaaag cgacatcaat atcgaattct tctgcaagca atcctagatc
     5581 actgaaataa gcagtatccc ctgcatgata aatcgttttt ccttctgctt gaataataat
     5641 tccgctaggc tcgcccatat actgaacgat accgtctact tcatagcttg aactatgctg
     5701 tgcatgtacg aattttacca cgccgaatgg aaacgcatgt tttccaccga tattcatacc
     5761 gaatgaatct actcctctgc tgttcgcata atcagcaatc tcaacaatac tgataattgt
     5821 ggcttgattt tcttttgcaa taggaatcat atctcctatg tggtcactat gaccatgagt
     5881 aatcagcaac caatctgtct ttacttcttc tgcagttaag tctgtcaatg gatttcctgt
     5941 aataaacggg tcaatcatca cgcgttggcc attttccatt tcaattgtta aacaggaatg
     6001 tccatgatac gtaattttca tattttacac ctcgcgtttt ctctgtatct tttttattaa
     6061 aaacgatttc atcacttgca catcaatgaa aaaatatagc tgctaaatat attttcattg
     6121 gattgatagt acagtatgta caaagcaatt gtaacagata atacaggatg caccaagtac
     6181 caagcagata taatctcaag tttgaggtct ctgctctgct tagcttctct tctcgtccct
     6241 taactaatta aatcagcgta aataggatgg acgatctttt ctaaatcggc tacttttatc
     6301 cgaaggcttc tcccgatttc accgcttgac acaattactt cttctttttc ttttaaagaa
     6361 ctgtctgcta aaaaacaata atcaggatgt gcgagtttta ttccaatagg ggtgtttgct
     6421 ccgtggggat atcctgtatg cattaataca aaatccattc caggaaaacc aactttacga
     6481 tctttcgtca cttgtctcgt ttttttgtaa tctaagtgat cgttcaaagg aatcaatgcg
     6541 atgatcaccc cagttttgtt tcctttcact acgagagttt tacatatcaa agctggatca
     6601 atccctcttt tttgtagttc ttctgtgaaa gattcttcta ccacatgact aaaatcaagt
     6661 ggttgatagc tgatcttttg ttcttctaaa taacgttcga cttttgtttg actcatcatt
     6721 gattcactcc ctttattcta tcgtttatta taaagcgagg gatgctgtat tcaaaaatta
     6781 tttgtttttc gcaaaaaaag agcaagacga gcactctata gagaggttca tcttactctt
     6841 tttgtttatt ttttatttcg acgttgagta gcttccataa tgactggcaa gatcactggg
     6901 cggcgttttg tttgttcaaa taagtaacgg cctaattgat ctcggatgtc ttgctttaat
     6961 ttgctccact caaaatcatt gctttctaaa tgtttctcaa cgattgtttc aatcatattg
     7021 ctgctttctt tcatcagatc acggctagct tttacgtaaa cgaatccccg tgaagtaatt
     7081 tgaggttttg aaatgatttt cttttctcgc cgactgatag tgacaaccgc tacgaaaatc
     7141 ccgtcttctg acaagatttt tcgatcacgt aaaacaatgt ttccgatatc acctatgcct
     7201 aatccatcaa tcattacatt ttctgcactt gtagttcctg caacactcat acgcttgttc
     7261 ttatattcca gcacgtctcc gcgtccagta atatagatat ttttgtatgg catccctact
     7321 gcatgagcca aatctgcatg agcagctaat tcacgatact ctccttgaac tggcacaaaa
     7381 tatgttggtt tcatcaaatt gatcattaat tgcaagtcat taggattggc atggccagaa
     7441 acgcgcatat tctcagagat caacttcact gttccaccag ctcgataaat gatatcttcc
     7501 gttttcgcca cgatggtttc catcgcgatg cttggcgtag tagtgatata aaccaaatct
     7561 ccttcttcaa tcttgacatt acggtgtgta ccgtttgcca ttttttgcag agacttgatg
     7621 ggttcaccca ttcggccagt ttctaaaatg atcagttctt ctggatcata gtttttcatg
     7681 tctttcaacg taacaagcag atcttcactt ggtaattgaa gtttttctaa acgcatcgct
     7741 gtgcgaataa ttcgttcgaa atcttgacca gtcaagacaa cttttcgttg tgctttttct
     7801 gctgcatcca atacttgttg cactcgttgc aaattacttg ctacacaagc gacgatgata
     7861 cgcccttccc aatatttgat cgtatcaaat acttcatctg ctatttgggc ttcagaagct
     7921 acaggctgag gattttctgc atttgccgat tcacttagta aggctaagac tccttctttg
     7981 ccgatttccg ccaatcgtgc ataatctgtt tgatacattg ggatagccgt ttggtcaaat
     8041 ttaaaatctc ccgtataaac gatactacct tcttttgttt tcagattgat ccccactgaa
     8101 tctggtatcg tatgagtggt acggaaaaag ctcactactg tttcaccaaa gtctatttct
     8161 gtatgctcat ctaccacatg gaaatcctta aatcctttgg ctgcttcatt acgactgaca
     8221 ttcaattttg ctaactcgat cgtcaactct gtcccgaata ctggtacaga aatttttgac
     8281 aacaagtaag gaagtgcccc gatcgcatct gcatgtccat gtgttaaaaa gacaccagct
     8341 acacgatcaa tgttttcttc taaatacgta aaatcaggga ttacgacgtc gatccccaac
     8401 aactcatttt caggatattt tagcccacaa tctaaaataa aaatatcctc gcccacttct
     8461 gcgacataca tgttttttcc attttcacga acgccgccta atgggataat tttaatggta
     8521 ctcacaaact tcacctcttc ttatgacatc caagtttaaa gatgtcgatt ttcttcttta
     8581 tctaaatata tgagccattc caatgaagca tatgcaaaat cactatttct caacttatgg
     8641 agaatcagca ggaaaattac cagcacatcc gcgttctgac agttggatgg gcattttatt
     8701 ttggatatac ttcattaaca aaatggatct tttgcgaaac gctatagcca gaatacttat
     8761 ataagtattc agctgttccc cattattata caacaaataa actaaatcgc tagtatcagc
     8821 aattgttaga acaactatgc acgtaaaaat ccctctgtag catctaggat ttcttttgct
     8881 attcctcttt catttcttct tcgtcggttt gaaacatact tttttcattt taccacattt
     8941 tttgcaataa ctatagtgac tttttatata tacagaacct catgttgatg gtgtgcaaac
     9001 agcataagat ttaagctagc tttaagatag gttgtctatt atcaaatgca tctactagga
     9061 gggatgcaaa atatgaattt tatgaaacgt gcatggaaaa gtacaaaagt aaaatgggga
     9121 cgttcattgt tattgtttgc cgtgttcaca gccattctta tttttgtgct agctggcctc
     9181 acgattcgaa gcgctgcaga agaagcagcg aatcaagcac aaaaagaagt tggagcaaca
     9241 gtgactttaa gtgccaatcg agaagcttca tttaaaaaac aggaacaaac ttcttcaagc
     9301 ggttctacca ctgaagaacg tccagatcca ggcagttttt ctcttacacc agtttcctta
     9361 acggacgcgg aaaaaatagc tgaattagat aatgtaaaga gttattcctt tgaaagttcc
     9421 gcctcagcta tggcaaaaga tggaataact gccatcacta gttctgattc aagtgaagac
     9481 agttcagaaa ctactgatgc aggcggtgaa cctgctggag gaatgggcgg gaatccgcaa
     9541 atgactcaag cagactttca aatcagtggt gtgtcagcaa ctgcacaaaa tagtaatttt
     9601 tctgatggaa cagcaaaaat cacggatggt gaaggaatag atgacagcga caaaggcaca
     9661 aacaatgtag tcattgattc tactctagca gaaacaaatg acctggcagt cggagatact
     9721 ttcacaatca cgagtactga agatgaagat accacttatg aaatgacgat caaaggaatt
     9781 tatgaatcca gcgaaacaag cggcagtatg ggtatgaaat tcaacttcat gaatccgtcg
     9841 aatacacttt acacttctta cacttttgca aatgaactaa atgggacatc tgacgataac
     9901 acgatagatt ctgctattta cactctatct gatccagata aaatggatga gtttctcaaa
     9961 gaagctgaga agctgatcga cacagataca tttagcttac aatcaaatga ttccatgtat
    10021 cagtcaatgt tggaaccttt aaacaacgtg gcaagttttt ctaaaaatgt tgttttacta
    10081 gtggctgtag ccggtattat catacttact ctgatcatta tgattaccat tcgggagcgt
    10141 cggcatgagt taggcgtctt gctatcttta ggagaatcca gaagtaaaat cattctccaa
    10201 ttgtttaccg aagtggctat ttgtatgatt cttgcattag gagttgcttc gcttagcgga
    10261 aacgtcgtcg cgaatgccgt gggacagcag ctacttgacc aacagacaga aaccacgaca
    10321 caaaaccagc aagctcctgg cggtggacaa atgccaggta acagacaaga acaaggcggt
    10381 caacgcgatg gtcaacaagg aaccacgaat aatccatttg ctgtttctga tcaagtcagc
    10441 gaattggaaa tcaatgtcaa acctgcacaa ttgggactat tagccggagt agcatttgga
    10501 atcagtctgt tttctgtctt tttagcttct atagggatat taagattaaa cccaagaaaa
    10561 atcctattaa actaaaggag gaatttgatg ttacggacag aacaattggg attttggtat
    10621 caagatccgc aagatgcgct ttataaagat gtagaactga atttcgaagc tggaaaaatg
    10681 tacgctattt taggagcaag tggttctgga aaaacgactt ttctttcgct tatttctgga
    10741 ctagatgcac caaaagaagg aaaggtatac tatcaagatc gttcactgaa caaaatcggg
    10801 ttaagaagtt atcgtaaaaa tgatgtctcg attattttcc aagcttacaa cttgcttcct
    10861 tatttatcgg ctctagacaa cgtcatagca gcaatggaaa tcactaagac caagcaagag
    10921 aattatcgac aagttgcttt agatcgactg gctcgtgtag gaattgatga aatattagca
    10981 aaaaaacagg tgaccaaact ttcgggaggt cagcagcagc gagtcgctat cgttcgtgct
    11041 atctgctgtg aacatcaact aatcgtagcg gatgagccta ctggaaatct agatgaaaca
    11101 acttcacaag atatcgtcaa acttttccaa gaaatcgccc atgaacaaaa tcgctgtatt
    11161 atcatcgtga ctcatgagat tgaagtagca aaaagctgtg atgaagtctt tgaactaaaa
    11221 aataaaaaat ttcaaaaaat cgaagtttaa aaaacaggat aatgatcaaa taaaaaatcc
    11281 gaaccatatc tgtctcgcag atatggttcg gattttttat ttgcttgatt caattagtaa
    11341 taagtggttt ttattttttt tcgtcttttc gagaaaactt gtctttgact tcgtcgccta
    11401 aatttcgagc tttttctttg atgtcagcac ctagtgattg tgctttccct tctgtttctt
    11461 tgctcttgtc gtctgttgct ttgccgtatg cttcttttcc ttcaccgact actttatctt
    11521 tcatatcttt agaacgtcct tttaaatctg ccattgtgct tcctcctctt agtttgttta
    11581 ttgatctacc ttcagggtaa catcaacaac agatacagac aaacagaacg ctcgttttct
    11641 aaaaaagata gttagagctg ttgattaaaa acttttttcg aaagtccccc atactttgac
    11701 ttctttttct tggaccatta tttctccttt agcgataact gtatcaatat caagcgtttc
    11761 tttatccaag atacacagat ctgcatcagc accaatttcg atttttcctt ttttatccaa
    11821 tttcaatata cgtgctggat tactggttat agctcgtaag gcgatttcca aagggatact
    11881 ttctttttgc acagcttctt tgatccctac tagcaatgcc ttggcacttc ctactcctaa
    11941 ccctaggaaa tgattgtttt catcaaaata aggcaagctt ccttgtccat ctgaggtcat
    12001 cgtgaaatta tctaaagaaa catcttcctc gatcaatcgt ttcagtgctt tgctgaagcg
    12061 aacttcacca tctgtcttct cccaaaaatc aggatcttcg ctagcagtga agtcaatcgt
    12121 gccaccacgt ttggcaaatg ccacgcaagc ttcaaataat tctggtgtgc gatttgcatg
    12181 agtaggcaaa aattgtgtga tcggaatttc tgtttcttct acgattcgtg tcaatagctc
    12241 tagctttctt tttcctccgc ctaagtggac attgatgatc ccagcttttc cggataacat
    12301 gcctcctaca cgagcatctg ctactgcacg agcgaactct tcaaaacttg gctgagaaga
    12361 acggtgatcg gatactgcga tttctccgat tccgataatt ttatcaattg tcaaaaagtc
    12421 tttgatgata gaatctgtta ctgtttttac cggcaatcgg tacgaacctt cgtatacata
    12481 agtggagatt ccttctgctt ccagaccttt cgctttgctg atcaacgcag tcatatctct
    12541 accttctccg tctgttccca aacaaccgac aactgtcgtt acaccagctg tagtcaattg
    12601 gctcaatgtg acttctggcg ttcgattttg ataaccgcct tctcctcctc cacctaaaat
    12661 atggaaatga cagtcaatga aacccggggt tagtactttt ccctcacctg aaagttcttc
    12721 tacttccact ccttcgtagc cgccttctag ctgttcttct acagccaaaa tcttcccacc
    12781 ggcaatcagt acatccattt ttcctaagta atctggtgca aacacttcaa tttgtcgaat
    12841 gattttcata catttttcct ctttctttaa gtataactga tgataggatc ttaaaaaata
    12901 atccaaacga taggatcttt actcctttga ttttaccgta aaatgacggt tcttggagtc
    12961 cttatctact gtttggatta cttttttatt tacaggtctc cagtactact gcagacctat
    13021 tgcaatggca atcatgatga aaatagttcc taaaacaaac aggaatcctt gcatcttgat
    13081 ttggaatctt gcccattttg cccattcgat ccgtgcaaca cctagcacac caatcaagct
    13141 ggctgatacg ggtgtaaaag cgtccataaa acctgaacca agctgataag caagaactgc
    13201 tacttgtctg gatacaccta ctaaatctgc cagtggagcc atgattggca tagtcaaagc
    13261 tgcttgtcct gaatttgatg taacaaccaa gttgaacaaa ctttggaaga tgtacatcgc
    13321 ccaagcacca atgacagctg gaacaccggt caacgccgtc ccgatcccat gcaaaatcgt
    13381 attcaaagct gaaggaacag atgcatcaga tcctcccaag acaagaagaa tccctttggc
    13441 catacctact acgattgctg taccagctaa atcagctgca ccagattgga aagaagaagc
    13501 gatgtcattg actttcattc catctaattt gaaaagaaca gcaatgattc cagcaactaa
    13561 tcccatcaca aagaattgag aagcgatttc tggaatgtaa tagccttttt gagtcacgcc
    13621 ccaaacgatc cagacaagga caactaacat ctctaataag atcaatttat gcccaagcat
    13681 gaatttttta ttttcatcag atgtcttttt gatatgagaa cggaaataat catctgattt
    13741 gtaagtcaac gaactttctg gttttttacg gattttttct gcatatatca tcatataacc
    13801 agcggctaat gcagtaacaa ctacccacat aatcaatcgg aatgtcgcac cagacaacac
    13861 ggggattcca gcaatccctt gagccaccgc tacgctaaac gggctcatcc aagaggtcgc
    13921 atttccgact tgagatgcga cataagtcac ggttactgct acgatcgagt catatcctaa
    13981 cgcaatgaca aatggaacca tcaccattga gaatggaatt acctcttctg ccatcccgaa
    14041 tgtagcgcca ccgaatgaaa aagcaaagaa taatagcggt aaagctaaac gctccagtcc
    14101 cttagtttta ctgataaacg catagatacc tgcgtcgatc gctccggtac gcataataat
    14161 accaaaagca cctccaacaa caaggatcaa tgcagcgatc cctacagcag atccatactt
    14221 atctccagat actagtcctt caaaaacaaa atttaagaaa ccaaagccgc caaaatcatc
    14281 tgttccccat attttggctg ttttatgcaa ttttttagaa gtatcataga tattttcgcc
    14341 gtactgatcg taaagcacat catctgtcaa ggagatttca tctagttttt cctgcgtaag
    14401 atttttttct ccgtcagcaa tgactttttc caatcctttt tccggaacat ccagtttttc
    14461 acgttctgct ggatgatctt gcagttcttc taattgatca aacacatacg acttatccag
    14521 ctcgtaagca taacggaagg aatcttgacg taaaacagta cgagtacttg tttcgccatt
    14581 tgcatctttg tattcaatat cttgcgtgct gaactttcct gctggaacga caaaagtcag
    14641 tatccatgca aagatgacta caccaaaaat aatgacatac gtatggggtg tcgataattt
    14701 agtgaagtcg cgtttcggtt ttcctgaaga atttgttttc ttcaaattta tttcttcctt
    14761 tcttttttca gtaattttga tgaataaaaa atggataatc aaatgaatta tacagtaaaa
    14821 atgacatttc cacatgaata atttattaga aaatcacttt tttctatttt gtttttttct
    14881 cctatgtatg attatttttt tctctttcca tttatattaa gtattttatt tctctgtttt
    14941 tccttaaaat tcttttttta tttttagatt agagaaaatc tcgtcctgtt tttttcattt
    15001 tacaatcatt gaaaaattga atttcattgc aaaaaaaata ataaacactt ataaatatcc
    15061 attatctatc cttcaatttt tgtgtatttt atttctatta gaaaaaggta tcctgttgga
    15121 caattttgtt atatcaggta aacttcattg caataatgat aacaaatgta ctataattac
    15181 tttgtaaaga gttttgggga agactctttc atcaacgtgt attaagagac aagaaagaat
    15241 ccatatggat tctttcttgt ctctttttcc actacttttt atttttctga cactacatat
    15301 tcttataact catttctcat aactgtgaaa caacttataa aaataacaaa ctttaaacaa
    15361 aacaagctta aaatcaaaga tgatccatct taaaataact aaaacaaaaa aagtagtcgt
    15421 tgttaatcct cattaataat cgttttcttt ttttcaaacg ctccattttt atgtggaaca
    15481 ttattttttt aaggaacttt gtcaacatgt ggataactat tttccacttg tgtcctggac
    15541 tagctgtttt gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg caagagaggc
    15601 tgttccgcag atcgctggat ctagatttgg caaagtcaag actgcatcat gtaaattggc
    15661 gtggttgtcc aaaaccaaat cagcaaattc aaataatcgc aaaccactgc tatgttgcga
    15721 tttgatactt cgtgacaaat caaatccagt aacaatgatt aatggatgac cattttcttt
    15781 gatccactga gctagttcga tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa
    15841 aatgtcttct gatttgacag aaagctgatc aataattgcc tgtcctgcac cttctagcgt
    15901 ttcatagaca cctccggctg gattttcaat caatatactt ggtacaagat ccggggtttg
    15961 atcccaaaat tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctacc
    16021 accttgatta atttgtttcg cgatccaagt gccagcttga agcatcagcg gctcttctat
    16081 ccttaatttc tcttccaatt ggtgataata gtcaaaataa cataaactca tttatctctc
    16141 ccgcctagct atacttgaca tgaatagaaa cagctgccta atctaaggaa gtacataaaa
    16201 aaacagtagt tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt
    16261 gaacgaatac gcccaatacc ttgacatagc aaataaaaca acgtgcagac catggaaaat
    16321 atctacatag gggtagaatt ttcaccctag attaggtaca ataaattagg tcgatgcact
    16381 gcattgacta tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc attccctgat
    16441 tcttagcgcc aactaagggt cagggagcct ttttttattc agctgtttct atctgaattt
    16501 attgtaagta atctcttatc caagtgcaag caagaaaata caaaaaaaca ttggtatgat
    16561 ttatatatat ataaataaca taaaaaaagg aatggcttat cccgatacaa caagcattcc
    16621 tttaaaaaag attcttttat ataagtgaaa aaagcttttt tccaccaatt aaataaatct
    16681 ctcatctcaa tagtttatcc attattttat ctttttttgc gtatacctac taaatattac
    16741 cgtcttcttc tcgttcttct actttgttcg agagaatctg tactagaaaa agtagttgta
    16801 tgtctacctt ttctgcctat ccgagacttt cttttcatat atgggatata aatgattaga
    16861 tacagtacag caaaaaataa agcaaatacg caagcttccc aactacctaa cgccaattca
    16921 accatcaagg tagcaacccc aacggatgct gcataccaga taaaaatcag aaacgtttct
    16981 ttttttctga ccatgcgaaa catgataaat agactaataa gcaatagtgg caacacaaat
    17041 atccaattgc ttccttccat cagccacttg agtatatcaa gcagcgactg tgcaacagcg
    17101 actaattttc ccatagtgaa cacacacctt tttttataat taaaaaattc gcctttttat
    17161 tctactatga atcttctggc ttggctttca tttctcttat gtaatcattt tcttttatac
    17221 aacttaaaaa aaaattaaac cattagcata atattttctg aatctattca cgaaagtctt
    17281 tttctatctt tcgttttcca cgactcaact gatctagagt caaaaaacta aaacgacagt
    17341 tgcgcaaatg acgcacaagc tgccgcttta ataacaaata gcctttcatt ctcagcaggc
    17401 cacttatttc cagttaactt tctgttctta gctttttact caactggata atatatgcac
    17461 ttaatgaagt cccaatttcc gaatgttgaa gtccatattc aatatttgtc ttcatatatc
    17521 ctaacttatc gcctacgtcg tatctttttc ctttaaactc tctcgcatag accgcctctg
    17581 tctggttaag agtctgaagt gcatccgtta gctgtatctc gtttcctgct cctggttctt
    17641 gagtttctaa tatctcaaat attttaggag tcagtagata acgtccgata atggctaaat
    17701 tacttggtgc ttttcccatt tcaggttttt ctacaaaccc ttttactctg caacgaccct
    17761 tttcgctatc tgttttagga tcaataattc cgtatttaga tgtttcattt tctggtacac
    17821 gcatgacggc aacagcggaa gcttgtgttt cttcataatc catcatcaat tgttgagtca
    17881 atgggatatc atctttcatc aaatcatccc ccagcatgac gacaaacggt tcatttccta
    17941 taaatgcttt tgcttgaagc acggcatccc ccagtcctct tggatgagat tgacggatga
    18001 agtgaagatt gacttgagtc gtttcttcaa ctaaagcgag taattctgtt ttttcctttt
    18061 cccgcaaatt gttttccaat tcgatatttg catcaaagtg atcttcaatg ggtctttttt
    18121 cttttcccgt tacgatcaag atgtcttcga ttcccgaatc caatgcttct tccacaataa
    18181 actggatcgt tggtttatcc acgattggaa gcatttcttt tgccatggct tttgttgccg
    18241 gcaaaaaacg tgttcctagc ccagcggcag gaatgactgc ttttttgacc ttcacttgct
    18301 gttcctccta aacgacgatt aactattgat catctattta acgaagagaa aattcattct
    18361 ccatttttcc gtcacgtaac atgatttctt ttgctgcttt tttgatatct tctccattat
    18421 acaaaacatt atagattgtt tgagtaattg gcatctcaac acctagctgt tgagctagct
    18481 ctactgctgc ttttgtcgtt gccacaccct caacgaccat tcccatgttt tctagaactt
    18541 cttccagctt atgtccctgt cctaacaagt taccggcacg ccaattgcga gaatgaacac
    18601 ttgtacaagt aacgatcaag tcccccacac cactcaaacc gataaatgtc agaggattgg
    18661 cacccatcgc gacaccaaga cggctgattt ctgccaatcc tcgagtcatg attgctgctt
    18721 ttgcatcatc tccgaatcct aatccatgga tagcgccggc accaattgca atgatatttt
    18781 tcagtgctgc accagtttct acgccaatca catcattatt tgtataaatc ctgaaataat
    18841 cattcatgaa cagtttttgt acataggctg ctgctttttg atcaatactt gcagctgtaa
    18901 ttgttgtgat atcatgtact gccacttcct cagcatggct aggaccagat aatacaacaa
    18961 ttgcctgtct tttttcttct ggaatttctt cagccaaaac ttctgagatt cgtttatgcg
    19021 tatcttgttc tagtccttta cttgcatgta taatgattgg cttagtattc attttaccaa
    19081 taagttcctg tgcgactgaa cgaatcgctt ttgttggaac aacaaataaa atagcatctg
    19141 cgttatccac tgcttctgcc aattctttat atccgataat cgattctggg atcgtcaaat
    19201 caggtaaaaa atgctgattt gtatgatact tattgatttc atcaatctgt tcaggtacat
    19261 ttccccaaat acggacatca tgcccgtttt ctgccaatgt ttgtgctaaa gcagtgcccc
    19321 aagatccagg accaagaact gcgatttttt gtttcatgga tattcctcca aataaataat
    19381 tgataatttc cattatagta tacatttctt aaagttggga ctaatttcca ttgtttttaa
    19441 gtgtttgttt acttctttta tcgtttttac ttgaaataat caatgatatg ttacaatagt
    19501 ctacatttta tttttacact cgttatgctt tgcttgttcc taagaggaag cagttttgac
    19561 aatgttacta ccagcattac tattaggagg ttaggaaact tctcctagtg agtattgatt
    19621 taaaatacat aagagacaga ttgaacattt ctaaatctgt caggtcctgc aacggacaga
    19681 ttgtctctgt tgacggttga cataggaact tttgttttag tcaacattct ggatacaaaa
    19741 cgctatgtaa actggcgcaa ctcgttgtac ttggactttt actacggatc catgcaacaa
    19801 ctaaatagta ctattgcata acaaaaaatg gttggcaccc acatgtccca ttaaacagat
    19861 tataagttga actgtagata atgcaaaaaa tgtgttttat aacgtaattg atgccaatat
    19921 tttaaaaaaa taaagggagc agttatcgct gctctcttta ttttttatat tctttttatc
    19981 cagtctatcc taataatcaa actgacgata gtagcggcgt atctctaatg gatggcagtt
    20041 caactgctcc acatgagcat cgatgcgttg tgtcactaca tgcatcaata agaaagacaa
    20101 aacacgtccg cgatattttt cactgatacc ttctactgga tagtcttttg tgtctatcaa
    20161 tgtataattg ctgcaaattt ttggcaaaag tttagccaca cgttcagata acactctctg
    20221 ttcatcttcg cctaaaaata atgtgactgg ggttgtttct tctacgatct ctaacgtccc
    20281 gtgaagaaac tcggcagcat ggatagattt cgatggaagc cagctttgtt cttcccagta
    20341 acacatggca tatgaataaa cagctcccca ttgattccct gctccaataa aataatgcat
    20401 actatcacgt cgatgttttt ctgcaaatga taagccaaat gcatctgctt ttttctctgc
    20461 ttccactaac cctgttggta aataacgttc gagctgttca tagtattctg aataagcttc
    20521 gaattctcca ttcaaataca tcaatcgatc tgctgccata aaaaatttga tttgttctgt
    20581 tccaggagct ggataagtga ccacaaaatc gcatagctga gacaacttac tgttcgcttt
    20641 atcgataaat ccaatcagtg tcgctcctac tttcttgatt tgttctactg ctttcactac
    20701 ttcttgagtt gtaccggtaa cagaggacaa aataacgata gaatcttttg ttaatctttt
    20761 gtttccagta gtgtaatatt ctgcagcatg ctgtacaaag acaggcaagt tgcttttccc
    20821 attcatataa gtgacagctt gcatcgagga agcatacgtt ccaccgattc ctagataata
    20881 gacagcgtca aatttttccg cataaagttt atcgatgatt tcttctactt gcggacgtaa
    20941 agctagtgcg ccttttattg ctttgatttg ttcttcttca ttgaatttca gcatgatttc
    21001 cacctcttat aatttacttt tcaagactgt atcaaagtct tcaaaaatct ttttgttcaa
    21061 gtcaatacct ttagctactg cggttttata agcgaatagt tgaaacggaa cgacaagcgc
    21121 taatacagac agatattcat cacattctat tgctaatcca agtgttttct cgtttcttgc
    21181 tttttttgtg gtaatggtga gagtctctcc aacgtaccga ctcatatatc tttgcaactt
    21241 ttgcgaacgt tccttattta cactttcccc ttcaataaag aatagcaaat gcgaagcatc
    21301 tgcctccagg tatgggccgt gcatataagc ttctaactca aatccttgtg acggaacgcg
    21361 gattgtttca gtcaatttcg tctctgcttc tttagctgtt ccccagtttg gaccatatcc
    21421 aatggtgacg aaacgctgtg ctaaacgaaa taaactttga tatccatcaa aaaattcttc
    21481 cgttttttga ataatcgctg gtaaatggtt gattattttg cgcaactgtt gcatgtagtc
    21541 ttgttctatt tttttgctga ttttattttt acttattcca attcctatcc ctaagagtag
    21601 taactgtaaa acagtcgcag aatagccttt tgtaacgaag cctactgatt ctaccccggc
    21661 acctaaatca ataatttgtg cagcttctaa agcaagtgga ctttgcacgt catttgtgat
    21721 agcaacaaca ggtaaccctt gtttttgtat catttttact gcttcaacag tagaagcact
    21781 tttcccgctt tgtgacacag caatcacagt atcgattgaa ggagaaagtt ttccataatt
    21841 gaaaaaataa aaaggttctt gaatatcgac agtaagatca ccataagatt ccaatgctgg
    21901 cttcgctgct aaacaagcgt tataagatga acccgttgct aagatcagta aattcactgt
    21961 ttttttcata tgttctcttg tcgaaaaatc attctttttc aagatcgtac ttagtacttc
    22021 cttttcttca tagatataat cttgcatcgt aaacattttc tctgctcctt ttttaaaaaa
    22081 tccctatcca cgaaccaaaa attccaaaga aagctagtcc gatcaagatc gttaatggtt
    22141 tcacttcttt cttcaataga taaaacacta tgccaaatgc tccaagacat aagatatttg
    22201 gaacgatatc atcaaaaata ctttgaacag tcacagcatt ttctcctgaa ccgattttta
    22261 aaggaatatt gatggtgatc atcgtggcag tcattgctcc aacgaccatc agtccaataa
    22321 tagacgcgcc taatgtcaaa ctttccatca taccgtttgc ctggatcttt ttcaaaaaac
    22381 ccgttcctaa gcgatatccc caacctgttg ctagatagcg aaacagaatg tgcggaatat
    22441 tgaaaatcaa aatgaacaag atagggccta aaatatttcc ttgcatggct aaagaggtac
    22501 ccacacctgt ggcaatcaat cgcaaagttc cccaaaagaa agaatctcct aaccctgcaa
    22561 gaggtcccat caaagatgtt ttgatactat cgatcgcagt cacatcaaat tcaggatcat
    22621 ccttattttc ttcctccatt gcagccgtga ttcctaaaat cagtgtaacg atatgtgggg
    22681 tcgtgttaaa aaactctaga tgccgtttca aagctgctgc catttcttct ttcgactgat
    22741 ataacttttt caaaatcgga atcattgcat aaacataagc catattcatc tgacgttcat
    22801 aattccaaga aaactccatt tgaaaagaac gccaaaatac gtgatttagc tcttttttcg
    22861 ttattttgct gtttttgatc tcagaattct tcatcttctt ctacctcccc tgcagttgtt
    22921 tgaagtggta gttgacggac attcataata ttgactacca caactgctaa aatcgcacca
    22981 aaaatagcta ctccggtcac aggaatctct aagtaagaag ctatagcaaa acctaagaaa
    23041 aaatatggtg caacttgttt gttgatcagc aaacgagcaa gcatcgcaaa accaagagct
    23101 ggaatcaacc ctgtagctac tgacaacccc ttttgaacga agtcaggaat catatttaat
    23161 agatttccga tcgtattgct tcccaccata taagaaacca ttaccacaac accaagcatc
    23221 aaggaaagac cgataccggc aagcaagtgc atatgctcga ctcctttata atttccttct
    23281 tctgcataat tgtctgcttt atggttcata attggaatca aaagccctaa gtaaatattt
    23341 ttcaatatca atgtcaacgt agcaatcggc aaacctagta aaagcgcggt ttctgtaccg
    23401 gctccagctg taatagcaaa agccgtccct aaaatcccac cggtcacaac atcaggtgga
    23461 atcgaagcac ctactgagaa cgatccgata aatgctaact ctaaagttgc gcccataatg
    23521 atcccagttt ttacatcacc taacacgatt cctgtaaaca gccccgtcac aatcggacga
    23581 gaaagcaacg atgtccctag tgcatattct gattgtgcga caaatgcaac aattcctaat
    23641 aagactgctt gtacaatcat tgaggttctt cctttcttac aaaacttctc ttacatccac
    23701 ttttttatct gctggtactt gacggatttc tacttctatt tgtttgtcaa ttaatcgttt
    23761 caagcgttcc tcctcttctg gtaaaatatt taccgctttt ccgatgctgc gagtaccagg
    23821 ttttgccttg atccctccta agttgatggt tttgatttct ggatacgctt ctgccagctt
    23881 atatgcatcc tcgatcgatt ccactacgat gaataactta tatttatctg tcaccccgct
    23941 tttcaacgcg gcaatcgagt catcaattga tttgatcact agtttgactc cttgtggctt
    24001 ggctaatttg atcgttgtct ttcgtatctc attcgtaggt acatcatcat tagcaatcaa
    24061 aatacagtct gcgcctattg tttgtgtcca tgaaaaagct acttgtccgt gtaataaccg
    24121 atggtcgact cttgtcaata caatcatttt tcttcctcct aaaattcgtc ttcttctatt
    24181 ttctgattga gccgctcgtt acaaaactgg atcatttttt ttgaatcagt tagcacctgc
    24241 tggatcaaaa cagttgtctc tacttcagcg tccaaccgag atactagctc aatcaatagt
    24301 ggcaaattca atccggcaac gagatagaca tgatcccgat tgatatattg taagaattcg
    24361 ttgttgacac ttccgccaaa aagatccgtc agaacaatga tttctttatc tttgtacctc
    24421 ataaaaagct gccttactgt ttccgctaaa tcaaaattct catcagtata gcaatcgaat
    24481 acagtaatag aatgattttt cccatagatc agctctaatg aatctaatat ccctgaagca
    24541 aattttccat gggaagctaa gataatttct ctatccactt tttcatctcc tcttttcact
    24601 tacacatact aaaaaagcaa gttgcatgcc aaaattagtg gcaacaactt gcttatgttc
    24661 aaaattcttc ttctatcgag agaaactctg tatcttcact caaaatatca tagatatagg
    24721 caatctcaga attggggatc atgacactat aatgctcttc aatgacacta aacgcagatt
    24781 taatagcaga taacccttct ttttgacact gcttccattt ctctaatcct tgataggttt
    24841 cgattggcat ttgtcggatc aatcgttcaa tgagacagct cacatggaca tacaacgcta
    24901 attttttggg attgctgatg cttcgattca actgcacctc taattctctc ataaaatgat
    24961 cgatttctcc catcaccttc tctgtatcta gaatagtcac ggaatccagt actttttcca
    25021 atgaaaaatt acgaatgatg cgctgattga atatttcctt ctctgaagca ttcatagtag
    25081 tgtccaacca ttcagctagt acagacatct tttcccccga gatgaggtct tctaatgaaa
    25141 gatagtcgat attggtgata tcaggatctg tggtgccaat cattcctacg acttcgtata
    25201 tggaaaataa tgggtcactg tttttcttat cttttaattg ctgatattca tatgggatga
    25261 ttttcaactg acaagccgta ggtaagcttt tttctagcag ttcacttaag tggaccgccg
    25321 taccgattcc agtgaagcaa gtagtaatga gtgctttggg tttgttttcc tctggataga
    25381 gcagtcgcca ttctggctgg atctgtcgaa cagtctcttc tgctatctct tctaatgata
    25441 catttttttg caggcattcc cctactgtga ttgctaaagg tgtcgtcaca ttattcaaaa
    25501 tcaaaagcgg tacggataac tgttttttaa aaaactgatg gatttctttc agagatccca
    25561 tgtctactaa tatgatcaat ccatttgaaa catcgctgtt ttcgcaataa tcaaggattt
    25621 ctgaagcaat ctgctgtggt gtgacatcta gtggcatatc aaatgattca aatagatcct
    25681 cttgtaataa acgatttact acatttgcca tactgctagc agttgcataa ccgtgagcag
    25741 caatcatcgc ttttggcagt cgtttttctt ttgaccattt cgcttttttt agatagatcg
    25801 tcagtaaaat ccgatccata cttgtgtctt caatatctaa tttgatctta cttaattcca
    25861 gaattcgttg gacatagtga taactagatg gataagactg agaaacttgt tgttctaatt
    25921 ctttgattag ttggataata tcatgttctt ccggtaacca tttgatagtg cttcgctgat
    25981 agagatagta actcaaggca tagacactgt ttccatcgaa tttgatttga taggcagttt
    26041 ccatttgtcg aaaggtctct cggatagatt gtgtccaata aaccagcagc tcgtgttttt
    26101 cttgtctact ggtctcaaac agcaaaaaat caaataattg aatcacttct tgctttaatt
    26161 ttccttcgca agaatatagc agacagttat ttttcttgaa atcaattagg atttgctcaa
    26221 aacttttaac gattcgctgc tgttcgggct gttgcttctc cattaattgc tctaaggtct
    26281 ggtcttgcgt aatatagatt ccttgctgtg ctgtattttt agtaataggc tgcgcaagta
    26341 gttctttttg caaatgatgg atcgtgacat ggatttcagg ctgttcacgc tgttcagcat
    26401 atgcttttgc tgctgaaact ttgacgatat ttttcagctc tcctatgttt ccggataatt
    26461 tctgacttgt taaaagtgac aaagctggtc cactgactga caaagggctc cccatttttc
    26521 tttgttcttc gagaaaaaaa gagtaaatca gttctagccg ttcattacga ctacgctgat
    26581 taagtgcagg tagttcgatt cggataggta ttcttcgaat gaaagttgtt aagaaatgac
    26641 tagtaatctc ttcagtcgta gcaaaaaaga gacgtgtttt gatccggcgt gacaaattag
    26701 gttctcccat acgataaatc acaccttgat caagataggt gaacagtttt tcctgacctt
    26761 ctggtgttaa gcgatgtacc tcgtctaaaa acaagatccc accatctgct gcttcaaaag
    26821 cccctttttt attttcttct gcaccagtgt acgctccctt aacatgtcca aacaaattgc
    26881 tagtcaaaag ttcaggatta tctgcatatt gtgcacaatt aaaagtgatg aatggtgcag
    26941 aatcttctag cagatcatga agcagacaat actgatagac caaattaact aaataacttt
    27001 tacctgtgcc actttcgcct gtaatcaaaa gtggtaatcc gccatctgga tagtaaagag
    27061 ctgttttgat ttgttcgata ctttctttca aactggcgtc gtatccgatc ataaaagaga
    27121 ataagtcttt tggttgcgcg aaaaaaggct gttcctcttt gagatcatcg atagaaaaat
    27181 aaaaattcgt gcgtaactta aaaaattgtt tttcaaaagc ttctttgtgg aaataaatca
    27241 ctggtcgaga attgattttg actagtagtt gctcatcatt taactggttc aaataatgac
    27301 tgactgtgtt tctttttacc ccaaaatgat cagctaacct ttgtgctgta aagacctcgt
    27361 tcatatgttc taaatcgaaa aaggctgtct ggttctctaa gtaacataaa atttccttct
    27421 tcaacaactt attcacccct cctccatcat agaaaacgga ttcattttgt caataaaatc
    27481 tttaataaag aattgaaaaa tatatcttat tatagacagc agcctactta tcccttataa
    27541 tttttattct aaaaaacgta cagttccttc cagtttttat actggaattt caacatatga
    27601 aagaataagg aggctatgac tattcaatga aatcggataa atataaaaac aggaaacata
    27661 cttcctggaa aacttttcga gaaatcatcg gatcttatct cgctgtagtt gtatttttgc
    27721 ctttgcttta tatggctatg acagaacaaa ctcgttttat tggcgtagat acgcaatata
    27781 tagccgaaac aggattttta ttcatcttta tcttgatttt tgcgttctat aaatccttcc
    27841 atacaaaata agattcacag atttcaactt tcctgtctgt ttttagctat tacaaatcct
    27901 tatatgtggt ataacaacaa tttattggca taatgaggaa aaacgagtta tgatgctttc
    27961 agagagtaaa gaggtactgt tgtcaccatc ctttttattg attacgtaca ccactcttag
    28021 ttttttccca ttgattacga aaacaaggta gaaacattga tgttcctacc ttgttttctt
    28081 tttttatttt cagatcaaaa tctcaagtag cttttgactt gatgtcggga ggttgtcttc
    28141 gttccatcaa acctgataga atctgcgcca aaccatagtt ttctccacta ttatttattc
    28201 ctctaagata gtacccacaa taaacgaata aatatttttc tcattttcat actccttctt
    28261 tgaaaatcaa ttagatttct ctcaacccac ttttttacaa aaattacgta gcaaaaaaga
    28321 ttgcaagaac aaattaaaaa agaagtaaat agagtttcat ctataaacat ttacttgata
    28381 tattttttat gcagaattca tttatagaaa aattaagtaa aatagacgtt cctatgaatg
    28441 aagagactgg catgttttct cattttcttt tttttaaaaa aaatagtcta ttcaatttct
    28501 aaagcttctt cataataaaa ctttcattta tgaaaaggct tgttctcttt tcattctaaa
    28561 tttcctaagc tacaatgtgg tagtacttgt tttttcaatt gtggacaaat aaataatagg
    28621 aggaatcata tgattcagtt attacgttat gcaaaagact atcgtaagca aatcattttg
    28681 ggcccgttct tcaaatttct tgaagctgtc ttcgaacttg tcctgccttt aatgatggct
    28741 tccttgatcg ataatggact aaaaatgaat gaccgaggaa aaatcattga aatgggctta
    28801 tggatggtcg ctatgtctgt gattggtctg atttgtgcta tcatttgcca atactatgct
    28861 tcagtagctt cacaaggatt tggtacagag ttacgtaatc agctaatcaa aaaaatcaat
    28921 actttctctc acaacgagtt gaatcatttt ggaacagata ccttgatcac ccgaatgaca
    28981 aacgacatca atcagcttca gctagcttta gcaatggtca ttcgtttatt gatcagagca
    29041 ccgtttttaa gtatcgggtc agtggtcatg gcttttgtga tcgactggga agtaggtctg
    29101 tttttcttag cattattgcc tattttctca atcattttat tttttatcat caaaaaaacc
    29161 gttcctctct accaaaaagt gcaagaaaaa ctagatcagc tcaatgaaca cgttagtcag
    29221 aatcttagcg gtgttcgtgt catccgcgca tttgcgcaga caaaaaaaga aacagagaag
    29281 ttcgataaaa gcacaaatga tctagcgaac aattatttac gtgtttccaa tatctctgcc
    29341 ctattatcac ctgctacaac cttgatcatg aatgtcggga tcatctgttt gctgacagtt
    29401 ggcgggatca aagtaaatat tggtagcttg caacaaggac aagttcttgc tttgatcaat
    29461 tatatgaatc aaatgctttt ggcactgatc gttgtttcaa acctcgtcgt gatcttcaca
    29521 cgtgccgaag cttcaggaaa tcgggtgaaa gaggtgctag acacagaaaa ttcgattctt
    29581 gaagctgagg tcagtactac tcccgatttt tcttctgaag caattatcgc ttttgaccat
    29641 gttgacttcc gttatactcc tgattctggt ctttctctcc aaaatattac atttcagctg
    29701 aagcgaaata cagtccttgg aatcattggt ccaaccggaa gcgggaaaac gactttgaca
    29761 caattgattc cgcgtttcta tgatgtcagt tccggtacag ttataatgga cggaatggat
    29821 gttcgttctt ggcagctgga tcatctccgt cagcaaattg ctcttgttcc ccaaacttct
    29881 gtactattta ccgggactat tcgtgaaaac cttcaatggg gtaagaaaga cgccacagaa
    29941 aaagaatgtt gggaagctct gaaaatcgct caggcagaag aatttgtccg tcagcttcca
    30001 aatggtttag atacaaaagt catggaaaat gggaaaaatt tctctggcgg acaaaaacag
    30061 cgactgacga ttgcaagagc tttgattgcc aagccagcat tactgatttt agatgactct
    30121 ttaagtgcgt tggattatca aacagacttg gatttgcgta aggcgctaca gacttatctt
    30181 ggcagtacag tgattattat ctcccaacgt gtccgctcta ttcaagaagc acatcatatc
    30241 ttagtgatgg atcaaggtaa gattgctgct cagggtaccc atgaagaatt acttgctcaa
    30301 tcagcagaat atcaggaaat cgtcgcatca caggaggaag aatagttatg aaagtaaaaa
    30361 aattatcctt gcagacgctc cgccgattca ttccttacct tactgcctat gcaaaagaca
    30421 tttggctagc tgtgatacta ggaatcatca atgggattgc aacagttgtg atgacgttac
    30481 aaattggtca aagtatcgat caaatgattg gagaaggaca ggtccgcttc aatcgactgt
    30541 tccatttact tattctattc ggtggtatcg ttttaatcaa cgtagtcagt caatggctca
    30601 tccaagtgtt aagcaatcgg ttagcttact tatccgttgc aaaattaaga aaagatgcat
    30661 ttgctcatct gaatcgattg ccacttcgtt attatgatca aaactcccac ggaaatatcg
    30721 tcagtcgatt tacaaatgat atggacaata tttccattgc aatttctgcg gccttcaatc
    30781 aattattttc tggagtggca atcatcattc tttcgtttat ttttatgatg cgtctaagtc
    30841 cacttctgac attggtcgtt ctttgttcta caccaattat tttccttgtg agttggatcg
    30901 ttgctcgtgc ttcacaaaca aatttcgata gacagcaaag gattattggg gatatctcgg
    30961 gctttgtgac cgaacgaatc ggtaaccaga aaatcgtgaa agcttttcag caagaagaag
    31021 ctagtcaaca gcaatttgaa ctgctaaatg ctgatctata tgaaaaagga caacgggcac
    31081 aattttcttc ttccttgaca aacccgtctt ctcgttttgt agatcacctt gcttatttat
    31141 cgattggttt agttggcggc ctattggcat tgaagagtgg ttcgtcaatc actgttggtg
    31201 tgatttccag ctttacgatc tactcaagtc agttttctaa accatttatc gaactttctg
    31261 gaatcacgac acaactgcaa accgcttttt ctggtctaga acgagcattt gatttgatcg
    31321 atcagccaga agaaaagcct gatttgccag atgcctatgc cttggaaccc aaaaatatca
    31381 aaggaaaagt ctcttttgat catgtttctt tttcttatga ccccaagcag cgtttgatcg
    31441 aagatttcaa ttttactgct tatccaggtc aaaccattgc tatagtagga aaaacagggg
    31501 ccggaaaatc cacacttgtt aatttgctga tgcgttttta tgacacaaca aaaggaacaa
    31561 ttacagtcga tgaccatgac atccgctcta tccaacgtga tacattacgt aaaagttttg
    31621 gaatggtgtt acaagatact tggctatttg acagtacatt gcgagaaaat cttcgttttg
    31681 ggaatccgga agcaagtgac gcagaaatca atgatgccct aaaagcctct tatatgtatg
    31741 aatttgtaga acgtttgccg gaaaaattag atactaagat tggcggtcaa gggttaaaga
    31801 tatcagatgg acaacgccaa ctgttgacga ttgctcgaac gatgatcagt aatcccccta
    31861 tgttgatctt agatgaagct accagctcag tcgatacatt gacagagaaa aaaatccaac
    31921 aagccttctt agcaatgatg gaaggaaaaa ccagttttgt gattgcccat cgcctgtcca
    31981 caatcaaaaa tgcggaccag atattagtga tggatcaggg agctattgtc gaaatgggca
    32041 cccatgatga gctgcttcaa aaagatggtt attaccgacg gctctatcaa gcacaattca
    32101 cagcacaata aaaacaagca gtcttctggg atgggaagac tgcttgtttt t
//
LOCUS       DACIRY010000022        31487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.022, whole genome shotgun sequence.
ACCESSION   DACIRY010000022 DACIRY010000000
VERSION     DACIRY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.022"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(76..543)
                     /locus_tag="IYE22_000839"
     CDS             complement(76..543)
                     /locus_tag="IYE22_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ9506070.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IYE22_000840"
     CDS             complement(663..2201)
                     /locus_tag="IYE22_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ9506071.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IYE22_000841"
     CDS             2281..2967
                     /locus_tag="IYE22_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ9506072.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IYE22_000842"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IYE22_000842"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ9506073.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IYE22_000843"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IYE22_000843"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ9506074.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IYE22_000844"
     CDS             complement(4453..5583)
                     /locus_tag="IYE22_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ9506075.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IYE22_000845"
     CDS             complement(5583..7766)
                     /locus_tag="IYE22_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ9506076.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IYE22_000846"
     CDS             complement(7763..8788)
                     /locus_tag="IYE22_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9506077.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IYE22_000847"
     CDS             complement(8833..9660)
                     /locus_tag="IYE22_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ9506078.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="IYE22_000848"
     CDS             complement(9657..10724)
                     /locus_tag="IYE22_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ9506079.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="IYE22_000849"
     CDS             complement(10750..11586)
                     /locus_tag="IYE22_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9506080.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="IYE22_000850"
     CDS             complement(11583..12452)
                     /locus_tag="IYE22_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9506081.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="IYE22_000851"
     CDS             complement(12522..13805)
                     /locus_tag="IYE22_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ9506082.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="IYE22_000852"
     CDS             complement(13819..15504)
                     /locus_tag="IYE22_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ9506083.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="IYE22_000853"
     CDS             15779..16804
                     /locus_tag="IYE22_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9506084.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IYE22_000854"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IYE22_000854"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ9506085.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IYE22_000855"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IYE22_000855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ9506086.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFVDQTNPLGELTYKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="IYE22_000856"
     CDS             25476..26444
                     /locus_tag="IYE22_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9506087.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="IYE22_000857"
     CDS             26462..27367
                     /locus_tag="IYE22_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506088.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="IYE22_000858"
     CDS             27364..28170
                     /locus_tag="IYE22_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9506089.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28327..29295
                     /locus_tag="IYE22_000859"
     CDS             28327..29295
                     /locus_tag="IYE22_000859"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ9506090.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="IYE22_000860"
     CDS             complement(29612..30136)
                     /locus_tag="IYE22_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ9506091.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..>31487
                     /gene="scm"
                     /locus_tag="IYE22_000861"
     CDS             30423..>31487
                     /gene="scm"
                     /locus_tag="IYE22_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ9506092.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8834 a         6801 c         5928 g         9924 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctccct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtatgtcaat tcgccaagag ggtttgtttg atccacgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt ttccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttgcttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatca
//
LOCUS       DACIRY010000023        31115 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.023, whole genome shotgun sequence.
ACCESSION   DACIRY010000023 DACIRY010000000
VERSION     DACIRY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.023"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..399)
                     /locus_tag="IYE22_000862"
     CDS             complement(<1..399)
                     /locus_tag="IYE22_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HAQ9506093.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIW"
     gene            complement(489..1034)
                     /gene="hpt"
                     /locus_tag="IYE22_000863"
     CDS             complement(489..1034)
                     /gene="hpt"
                     /locus_tag="IYE22_000863"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ9506094.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(1034..2479)
                     /gene="tilS"
                     /locus_tag="IYE22_000864"
     CDS             complement(1034..2479)
                     /gene="tilS"
                     /locus_tag="IYE22_000864"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ9506095.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(2599..3069)
                     /locus_tag="IYE22_000865"
     CDS             complement(2599..3069)
                     /locus_tag="IYE22_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ9506096.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(3115..3540)
                     /locus_tag="IYE22_000866"
     CDS             complement(3115..3540)
                     /locus_tag="IYE22_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ9506097.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(3607..3876)
                     /locus_tag="IYE22_000867"
     CDS             complement(3607..3876)
                     /locus_tag="IYE22_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ9506098.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(3887..5482)
                     /locus_tag="IYE22_000868"
     CDS             complement(3887..5482)
                     /locus_tag="IYE22_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ9506099.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(5497..9018)
                     /gene="mfd"
                     /locus_tag="IYE22_000869"
     CDS             complement(5497..9018)
                     /gene="mfd"
                     /locus_tag="IYE22_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ9506100.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(9035..9595)
                     /locus_tag="IYE22_000870"
     CDS             complement(9035..9595)
                     /locus_tag="IYE22_000870"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ9506101.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            9948..10922
                     /locus_tag="IYE22_000871"
     CDS             9948..10922
                     /locus_tag="IYE22_000871"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ9506102.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            11092..12492
                     /locus_tag="IYE22_000872"
     CDS             11092..12492
                     /locus_tag="IYE22_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ9506103.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(12570..13292)
                     /locus_tag="IYE22_000873"
     CDS             complement(12570..13292)
                     /locus_tag="IYE22_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ9506104.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(13371..13877)
                     /locus_tag="IYE22_000874"
     CDS             complement(13371..13877)
                     /locus_tag="IYE22_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ9506105.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            14000..15799
                     /locus_tag="IYE22_000875"
     CDS             14000..15799
                     /locus_tag="IYE22_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ9506106.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(15867..17441)
                     /locus_tag="IYE22_000876"
     CDS             complement(15867..17441)
                     /locus_tag="IYE22_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506107.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(17696..18205)
                     /gene="mreD"
                     /locus_tag="IYE22_000877"
     CDS             complement(17696..18205)
                     /gene="mreD"
                     /locus_tag="IYE22_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ9506108.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(18209..19063)
                     /gene="mreC"
                     /locus_tag="IYE22_000878"
     CDS             complement(18209..19063)
                     /gene="mreC"
                     /locus_tag="IYE22_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ9506109.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(19218..19856)
                     /locus_tag="IYE22_000879"
     CDS             complement(19218..19856)
                     /locus_tag="IYE22_000879"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ9506110.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(19857..20507)
                     /locus_tag="IYE22_000880"
     CDS             complement(19857..20507)
                     /locus_tag="IYE22_000880"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ9506111.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(20520..21419)
                     /gene="rsgA"
                     /locus_tag="IYE22_000881"
     CDS             complement(20520..21419)
                     /gene="rsgA"
                     /locus_tag="IYE22_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ9506112.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(21584..23653)
                     /gene="pknB"
                     /locus_tag="IYE22_000882"
     CDS             complement(21584..23653)
                     /gene="pknB"
                     /locus_tag="IYE22_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ9506113.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(23650..24390)
                     /locus_tag="IYE22_000883"
     CDS             complement(23650..24390)
                     /locus_tag="IYE22_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ9506114.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(24403..25761)
                     /gene="rsmB"
                     /locus_tag="IYE22_000884"
     CDS             complement(24403..25761)
                     /gene="rsmB"
                     /locus_tag="IYE22_000884"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ9506115.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(25761..26699)
                     /locus_tag="IYE22_000885"
     CDS             complement(25761..26699)
                     /locus_tag="IYE22_000885"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ9506116.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(26692..27183)
                     /gene="def"
                     /locus_tag="IYE22_000886"
     CDS             complement(26692..27183)
                     /gene="def"
                     /locus_tag="IYE22_000886"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ9506117.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(27198..29606)
                     /gene="priA"
                     /locus_tag="IYE22_000887"
     CDS             complement(27198..29606)
                     /gene="priA"
                     /locus_tag="IYE22_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ9506118.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(29859..30164)
                     /locus_tag="IYE22_000888"
     CDS             complement(29859..30164)
                     /locus_tag="IYE22_000888"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ9506119.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(30169..30783)
                     /gene="gmk"
                     /locus_tag="IYE22_000889"
     CDS             complement(30169..30783)
                     /gene="gmk"
                     /locus_tag="IYE22_000889"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ9506120.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         8538 a         6720 c         5735 g        10122 t
ORIGIN      
        1 ccatataccg ctgcttgatt cttcttttac tgttgtagca actttgtttt cttttgccag
       61 ttctgttact tggctcaatg ttgtatcatt tggcaaaatg attgttgtga aatgggtagt
      121 agtcgtatct gttgaaccta agatagctaa cccaccgctg ttagcgacgg tctgctgttc
      181 tttgtattca ccagtgatct tatatacacc atttgcaggc tgcacttcaa attctttgat
      241 cttgcctgct tgtagttgtt ctgtgaattt tgagtactcg atatctgacg atcgctggcc
      301 gttattacca aaaatgaaat aaacgaccat taccatcgct aagattacga ggacataata
      361 caaggcattt ttcatcatgc cgttgttctt tttgttcata gttgccctcc ctaagcgtca
      421 atttaattta acagtttttt atcaatcctg accattctat tgtaccataa tttaattgag
      481 atacagcatt aatttgattg ataaacacta ggtttcaata caccgatgta cggaaggtta
      541 cgatatgctt ctgcataatc caagccataa ccgaccacaa attcatttgg tacgttgaag
      601 ccaacataat ctgcttcgat atttactact cttccttcag gtttgtcaag tagtgtaaca
      661 attttgactg attttgcttt gcggtatttg aaaagatcca ctaaataagc aagtgttctt
      721 ccgctgtcaa tgatatcttc aacgatcaac agatcgcgac cttctacatt cgtatctaag
      781 tctttgacga tttttacttc ccctgacgag actgttgcat tcccgtagct tgaaacatcc
      841 atgaaatcca attccaaata cgtatcgata gaacgaacga tatctgccat gaatggtacc
      901 gctcctttta agacacctac tactagggga ttggtgtttt ggtaaatttc tgttaactct
      961 ttgcctagtt ctttgcaacg aacttgtatc tcttcttgtg agatcaaaat tctctcaata
     1021 tccttttcca acatcaggtt ctccttccaa ttgcttcttt ttgatatttg taaagaagta
     1081 tgtagtgtat tttatcagtt tcttcacgaa tactcaaata tgaaaaggca aaaggtatca
     1141 agctatatat atttcttttg gcatctgtaa tgatccaagc atgctgcctt tgtgaattag
     1201 gtactttctg atcgataaaa tatctagata ctttttttgt gagattctct gttaatttaa
     1261 tgcggtctcc tgcttttctt ttttcgacat acagttgcac atcagcagga atccaaatgt
     1321 catgagaaaa tagagaccat gaatctgttg ctttgatgtc ccctgcattt tcaggtttga
     1381 ccagaccaat ccattctgtt tcattcaaat atattccttg ttctggaatc agcaacaagc
     1441 gctggtcttc tgtctggcta gttgcttgtt tttctttttt agagagtaca acaaaatgat
     1501 actctctttt cagtatccaa ttattgccaa tactgagttg ccattggctt tttttctgtt
     1561 gccactggtc taaaatcgac tccatttgtt gttctttcaa aataattcct gtttttgaga
     1621 atgctttgtc gaaaaaggag taaaaaaaat agtatcgttc agcatctgtc atttcttcaa
     1681 tcatttctgc agaaaatcga aattgttcct cccactgttc gacttccttt tcgatcaact
     1741 gtcccatata tttttgaatg acttggtctg cccattggat ctgttgcgaa aattgctgga
     1801 aatgtctcaa cgcctgtgta ttttcttgtt tcaattgagg aactaccaaa tgacgaagcc
     1861 gatttctctg gatatctaac aattggtttg tctgatcctc aaagtaaaca agctgcgatt
     1921 ccgctgcata agtatatagg tcttctttgc taaaactcag caacggacga atcagtctgc
     1981 ccgtagcaaa tggttgtact tctttgattc cagaaaaagt atttaactgt cccccacgaa
     2041 cgattttcat taacaccgtc tcgatttgat catctccgtg atgagcagtc atcaacgtgt
     2101 catattgtcc ttcttccatc acgcgagtga attcttgata acgaaagtta cgagcttctg
     2161 tttctatggc ggatttggct ggatcttccc aaatacttac atataaaggg agattcttct
     2221 cttcacaata tgagcgaaga taggcagctt cttctgctga ttcttctctt aattggtgat
     2281 tgatgtggac gatacctaaa gaaaaaccga tttggcgttg cacttgtaaa agtaaatgga
     2341 gcaaaaccat cgaatcaaca ccacctgata ccgctgctaa aattctcgat ccttgttgcc
     2401 agaatccttg ttcattcccg tgccgaataa atgactgctg taatagcggt gcttttttcc
     2461 cgcctttttc aacgttcatc ctttccacac cctttcttcc taaaaaaaag agagcgggaa
     2521 aacgtctaaa acgacaatta acctgccctc tgttgatact atgacccaaa tagtcgcact
     2581 atcattattt gcggcttttt agctgcgacg tccgccacgg ccaccgcgct ttccttctgt
     2641 gtttcgcttc aatgaagata agcggtcatc gctatctttc aaaaatgaac tcatcaatga
     2701 atcgaagtct tctttttgag ctgaaggttg actgcgagaa aaagattttt tctgtgctgg
     2761 acgatttcgg ttgtcacggg gatctttttg ttgatatttt ttaggacggc tttcttctcc
     2821 agattcagca ggttgttcct gcgctttccg gatagatagt ccgatttttc catcatcatt
     2881 tactgaaata actttaactg ttacttcatc tcctacactt aaaacatcat ggatgtcttt
     2941 tacatagcca tttgacactt cgctaatatg aactagaccc gtttttccag cacctagatc
     3001 aataaacgca ccaaaatttg tgattcctga tacttttcct tgcagttttg ctcctacctc
     3061 gattgacata aaaaaaatgt tcctcctaat tatttccctt ttttatattc ctgcttattt
     3121 tgcagttgta gaactagttg attgatctgt ttcatttgtc gagctgtctg tatttgattc
     3181 taatatagga taaacttttt caccgtcttt tgagtaaaag aatttgcttc tggcaagttt
     3241 agctacatac tcatcatctt tcagcaatga aacatcttgg tgcagctgat caacgtctgc
     3301 gttgacttct ttcatttcag tatttgcttc gactctcaat tcgttcaatt gatgcatcct
     3361 ttgcatgtca tggaaaatct gcacacctat tatcgcgaaa atgaccaaag caccaacaaa
     3421 aattgcagcc agtctgcgtc ttcgaaaaat cagttgacgt tgttgttgct gaaattcagc
     3481 atactttttc tttgcatatt ccgtatctag cgcagcgatt ttattgcttt ttttcgtcac
     3541 ttggattcgc tccaatcttt ttgattgtct ttttcattat accaatatcg tgaaagcttg
     3601 tctaatctat ttctctagtg tttctgtgcg attttcagaa ataatttcgt acattttttg
     3661 tgcatcttct tttttggttg attcatgtaa ttccgtaatt ttcacttcca acgttttgtt
     3721 gccaaactgg actttcaaaa tatcgcctac tttgacatca ctggaagatt tcgccaattt
     3781 accgttgatt tgaattcttc ctttatcagc tacttctttt gctactgatc gtcttttgat
     3841 gattcgggaa atttttaaaa atttatctaa tcgcatatct tattcctcac tttttaattt
     3901 ttttatttaa tcgcaggatt ttcttaccaa aaggcaacat tagccattct cgtactgtaa
     3961 acagacgaag acgaatcact gcataaatga acacagaagc cccgatccca actcctatca
     4021 cgctgacaaa aaaagcagtg gaacgatgag ttacaggacc aaatactata ttcaatacac
     4081 cataatacag cagcagtact actgtcatac aaagaaggct gaacatcaac ttccgtccaa
     4141 aatgccgttc tttccaaaaa gcatttagct cttttgtttc aattcgaaca aaataccaaa
     4201 gcgtcacacc taatcctaaa agggtagaca ggcttgcacc tgctgttcct aataaaccgg
     4261 tcagaaaaga agtagtcaat ccttttacca atagtcccca tcctgcgcct tttaaagaag
     4321 ggcgaaacga gttcttgctt tgagcaatac tttgataagc ttggatgact gcagtgaaag
     4381 caatggaaaa aacaaataat acaagcgctg catttcccgc ataatcttta aacaacgcat
     4441 aattgatata agggagcagc aaggctaatc ccaaagacgc agctaatgct aaagcggtcg
     4501 tcaatctaag atagattttg gctgtctgta aaaactgacg atagatccgg ttggtcaaat
     4561 gacgagtcaa cgcaggcagg aatgtcgcac ttaatgcagt ggcaatcact aaacctaatt
     4621 gtactaaagg ctgacctcga tcatagaccc cttttgctat cttcgcaccg tattctgaca
     4681 gtccgtaaac ttccaaagca ttttttacaa agaaagagtc gatcagctgg aaaaagataa
     4741 gcaatccact ataaatcgaa accagtccgc cttctaccag aaaacgccgg atcaagggct
     4801 ttgacggctg tttgaacaat ggaaaccgtc ggaagcttaa cgctcctcca tgaatctttt
     4861 gttcatagta gtgaaggatc ccatatgcgc ataaaccgcc aaaaacagct ccactcattg
     4921 ctgccgtccc tgtttggtac accgtccatc caagctttct aaaagcaaac gcactacaga
     4981 caatcacaaa aacacgaacg aactgctcaa ctacttgaga aacagcagtt ggttccatca
     5041 agaatcttcc ttggaaattc cctcgataaa aagatagacc aggcatcagc aaaaaggtaa
     5101 aggaaactac ttggatcagc ggtttcaatt gtatgtctcc catcatccaa gcaatccagc
     5161 tgctaaatgc aaagacaaac aaccacaaaa agacacctgt ccaaaatact aagggataaa
     5221 gattttgcaa agcttgcttt cgctcgaaag gattctttct ttctgcaacg tactttgata
     5281 tgaactgcgg aagaccggat aaagctaaag tcattgctat cccataaatt ggataaactt
     5341 gctgataaac ataaaaacct tcgtcgccga ctaagttttg gaggggaatt cggtaaaggg
     5401 cacttaatac ttttgctatg aaagaagcaa tcgtcaatac aaatgctcct tgcatgacgc
     5461 gccgcatttc tttatgatcc aaatcattgc tccttcctac gctgacagat atttttgctc
     5521 acgcaacgct ttgacaaact tttggatttc ctgaagccat actgcttctt ccatttggtt
     5581 ggggatcgtc aagcgtatca ccatctgttc tttttcaacg cctagactgg cttttagttt
     5641 tgtcgctgtc agtgcttcga ataactgttc cacactatag cgttttgttc caactttact
     5701 tagagtaaag actatatgcg cctcctgttt ccttatcgtt tctaggagcg ctcgatcgcc
     5761 atccattttg atctgcccaa ttgtcaagag atgtgctact tcttctggat attctccaaa
     5821 acggtcgagc agatcatctt ctagttcatc tagcatttct tgcgaatcaa gttcacgaat
     5881 tcgtttatag atctcgattt tctgccgttg atctgccacg tatgtttcag gtagatacgc
     5941 atcgacaccg aggtcgatct caacagtggt cttctccact tgactattct ttccttgttt
     6001 acgagaaaca gcttcgctta gcatttgaga atacatatcg aaaccgactg catcaataaa
     6061 tccatgctgc tgagcaccta agagattacc tgcaccgcga atggaaagat cccgcatcgc
     6121 aatcttgaat ccagatccca gttctgtaaa atctttgatt gcctgaagac gtttttcact
     6181 gacttcgtta aggatcttct gctgctcata cataaagtaa gcatatgcga cacggctgct
     6241 tcttcctacc cgccctcgca attggtataa cgtggataat cccatataat cagcgttctc
     6301 aacgaacaac gtatttgcgt tcgggatatc tactcctgtt tcaatgatgg ttgtagtgac
     6361 aagaacatca tattgacctt caataaattc aaaaagcgta ttttctaatt gcgcctctgt
     6421 catttgccca tgagcataac cgatccgtgc ttccggaacg agttcttgga tttcttctac
     6481 tttttgttcg atcgtttcta cccgattata taaatagaat acttgtccac ctcgtcccat
     6541 ttctcgatgg attgcttcgc gaatagcccc aggatttttt tccataacat acgtttggat
     6601 cggataacga ttcgcaggcg gtgtttcgat tactgaaaga tcccgcacgc ctaacatcga
     6661 catatgcaaa gttctcggaa tcggtgttgc agtcagagtc aaaacatcca cttgtgcacg
     6721 taactgtttc aatcgttcct tatgttttac accaaaacgt tgttcttcgt caatgatcag
     6781 tagacccaaa tcgctaaatt caatatcttt tgacaaaatt cggtgggttc ctacaacaat
     6841 atcaacttga cctgtacgca gttgttcgat ggtttctttt tgttgctttc ttgttctgaa
     6901 acgacttaat agcccgatat ttacggggaa accttcaaat ctttccagca tcgtttcata
     6961 gtgctgctgt gccaagatcg ttgttggaac taaaaaggct acttgtttgc tttcctttat
     7021 cgctttaaag gccgctcgaa gcgctacttc tgttttccca taaccaacat cacctacgag
     7081 tagtcgatcc attggttttt ctttttccat atcccgtttg atctccgctg cacttctcaa
     7141 ctggtcatca gtttcagagt aggggaaggc attttcgaat tctttttggt aggcgtcatc
     7201 aggtccaaaa gcatatcctt tttctgactc tcttgcggca tataatttga ttagatcgtc
     7261 agcgatatcc tcgatttttg aagagacttt tcgcttggtt ttcgtccatt cacttccgcc
     7321 tagcttattg atgcgaggcg ctttggcttc agaggcgaca tatttttgga tcagattcag
     7381 ttgagtaaca ggaataaaca gtttgtcgtc gttttgatac aaaatagtca tatagtcttg
     7441 gtgaacgcca tctacttcta gcgtttccat cccaatgtat ttcccgatac catgattggc
     7501 atggacaacg taatctcctg cttttaactc attatagctt ttcaatcgct ctgcgtttgt
     7561 tactgtttga cgtctcgttt gttttttcgt cgttttttgg aaaatttctt tttctgtgat
     7621 agctactatt ttttcttgag gaagttcaaa accagcctgc aaagctcctt caactaattg
     7681 tacctgtcct ctgatcaatg catttttttc tgtttctact actaatatgt cttcatctcg
     7741 aagcatttgt tctgctttgc gtattctgtc ctttgtcgga ataaagatca caacagtctg
     7801 tttttgcttg cgccaacggt ccatttctgt tttcagcaat ggcatttgtc cgaagaactg
     7861 ttgcatcgtc cgatattgaa aattatggat tgcttggaaa cgaagatttc ccatcccttt
     7921 ttggaagagt gagaaaaagg ttgtggtgaa cttttccttc tgcacgattt tttgtatatc
     7981 tgctgaaaat gtctgctcag aaaacacccg caattcgctg attttttgag tctgccattc
     8041 tgccgcttcc ctttcgattt cccgatttgt ttccatgatt cgctgataat catcaacgac
     8101 tagtaaacta tttgaaggga aataatccaa gatcgtcaca gattcttggt aaagaagatc
     8161 tgagtagaat tttgcttctt ctgtcgggac acctgcttgc cagcttgaag tcagctgacc
     8221 gaagtaatct tgcaaaaact cgcggtctgc ttcttctgtg gtcgcagcaa gccgtttttc
     8281 tagcattttt tgcatttgct ctattccatg ttctagatcc tctggtccgt agacttgttc
     8341 actcgttggg gaaacccaga tttcttccgt tttttcgatt gagcgttgtg tttctgcttc
     8401 aaaataccgc atagaatcta tctctacatc aaatagttct acacgaacag gataggttgt
     8461 cgttaacggg taaatatcga taatgcttcc tcgaatactg aattctcccg gttttgcgac
     8521 catctcttct cgtacatagc ccatcaaagt caattggcga ggaagatttt ccacgtctat
     8581 ctcatcacca atatgccaat tcaattgcgc ctgtagccaa gttttcttat tcggcagtcg
     8641 tttgtgcaaa gatgcgactg gcaacaaata gatacctgct tttttttctt gaagtgcgtt
     8701 aagcgttgca actctctcgc ctcgcgcttc tggagaagaa aaggccattt cagcagaaag
     8761 cacttcatcc actggaaata aatggatctg atcttgcgga atcacatgtt gcagatcttc
     8821 tgctaactga ttcgcataat acaaatttgg catcagaata atcatgtttt tatttgtttt
     8881 cttaaacgct cgagcaaata gaagtgtttt cgctgagccc gctaatcctg tgattagttg
     8941 tctaggcatt ttttcttcta aattgctgac ccattctttt acttgcggat cttttccgac
     9001 taattgaata atatccattc cttttcctcc ttcctcaatt gaatcgattc atcgcatcca
     9061 cgaacgaatg cccttctatg agatattcga ctgctttaac agattgacac atgctttctt
     9121 ctatcaaagg ctgattttct ttgctgaaag gactaagcac atgctggact actgtttttt
     9181 tcccttctgg cctgccgatt cccaccttga ttcgatcaaa gacattcgta ttcaaatggg
     9241 aaataatact tttgataccg ttatgcccgc cggcacttcc cttttgacgg agccggattt
     9301 ttcccactgc cagatccaaa tcatcgtaga ttacgactaa ttcctctgga taaatcccaa
     9361 aataagtcat tagtgggccg actgcacgac cagattcatt catgaatgtt tgtggtttga
     9421 ctaaaaggat cttttctcca ttatgaaaaa attcggcaac ttctgcttcg aacggatttt
     9481 ttttgaaagt agcttgatgc tcgcgtgcaa ttttatccac gaccataaag ccgatattgt
     9541 gttttgtata ttggtatttg gtacctgggt ttcctaaccc gacgatcatt ttcatttttg
     9601 ttttccacct aattctaaaa agcagcacaa aaggctggac tgctaattag cggtccagtc
     9661 catcgagctt ttttcttttt tatacttgtc aatgcatagt ataacacaaa acaaggcgtt
     9721 tcggctaaaa gagaatcgtt aataaatgat aaagaaatta aaaatttctt caaagtttaa
     9781 tgaaataatt gatatataaa aatagtgaaa taacgcacat gtaagctttt acatataata
     9841 caaccttgtt atctgttata ttttatagcc aaatagcgtg acatggggct tgagaaatgt
     9901 tagcataagc atgggaaaaa acaaatcaat ggaaggattg atactaaatg actgcagctg
     9961 cagaaaaaaa agatcatcaa aaagttattc ttgttggaga cggcgccgta ggttctagct
    10021 atgcctttgc tctagttact caaaatatcg ctcaagaagt aggtattgtg gatatcaata
    10081 ctgctaaaac tgaaggagac gcaattgact tgtctcacgc tttggcattt acttcaccta
    10141 aaaaaatcta ttctgctact tacgcagacg cacatgatgc tgacttagtt gttattacag
    10201 ctggcgcacc acaaaaacca ggtgaaactc gtttagactt agtaaacaaa aacctaaaaa
    10261 tcaaccgcga tgttgtcaca caaatcgttg aatcaggatt taacggtatc tttttagttg
    10321 ccgctaaccc agtagatatt ttgacttact caacttggaa attctcaggt ttccctaaag
    10381 aacgcgtaat cggttcaggt acctctcttg actcagctcg tttccgtcaa gcacttgcgg
    10441 aattagttga cgttgatgca cgtaacgtcc atgcttacat cttaggtgaa cacggagatt
    10501 cagaattccc agtttggtca catgcaaatg ttgccggctt gcaaatctat gaatgggtaa
    10561 aaaacaaccc agaaatcgat gaagaagcta tggttaacct attctttggc gtacgtgacg
    10621 ctgcttacac aattatcgag aaaaaaggcg ctactttcta tggtatcgca gtagcattag
    10681 cacgtatcac tcgtgccatc ttagatgatg aaaatgctgt tcttccttta tcagtattca
    10741 tgaatggcga atatggattg aacgacatct acattggtgc acctgctgtt atcaaccgtc
    10801 aaggtatcca aaaagtaatc gaaattcctt taagtgacag cgagcaagac cgtatggctg
    10861 cttctgctaa gcaattaaaa gaaattctag atgaagcttt tgctaaacta gacgctgaat
    10921 aatctttgaa catgacggaa aacatctatt cggtgttttc cgttttttta ttttatcacc
    10981 cttatggttt ctaaattttt tcataaagta gagataacct tcgtcttttc ctctaaatta
    11041 tgctatcatg agaccgttcc accctaaact tttacttaag gagttgtcga tatgacaaga
    11101 tcttcgcatc gaaaaaaatc gaaacatgtt gctttgcttt cagttcttgg tgttctctta
    11161 gtgctgatca gcttttatac ctatcgcagt acatactaca caaagcattt tttgccgaat
    11221 acagaaataa atggaataaa tgtcagcaat ctaacagtcg aaaaagcaaa tgaaaaatta
    11281 aaagaagctt attctgataa aaaactggct attaaagaag atggaaaagt ctggcaggaa
    11341 gtaccaaaat ccgagctcgg ctacaaagat gatttcactt cggagctttc tccgatccta
    11401 aatgaacaaa acggatggac ttggggaatg acttatgtct cagcggcaga aaaacaagaa
    11461 atagatcctg tcagtcaaaa ccaccaaaaa ctagacacta ccgttcagac attgacaact
    11521 aagctgactg atttgaacaa agaccggaca cctactactg atgcaacgat cgaaaaagca
    11581 ggtgactctt tcgttatcaa gcctgaagtc aatggaaata cgatcgacgt cgatgctgcg
    11641 gtcaaacaat taaaaagtgc ggtaaactct ggtaaagata caattgaatt gactgaattc
    11701 aaagaaaaac caaaagtaac atctgaagac agctcactga aagaacaact cgcatccatg
    11761 aatgctatcg caaatgtaaa agctacttac agtataaacg gtgagacttt ccagatccca
    11821 tcaagtgata ttatgagttg gctgacctac aatgatggaa aagtcgattt agatacagaa
    11881 caagtacgtc aatatgtaac agatttaggc acaaagtata acacaagtac gaatgacaca
    11941 aaatttaaaa gtacaaaaag aggagaagtt acggtcccag ttggcactta cagttggacg
    12001 atccaaacag acagtgagac agaagcattg aaaaaagcga ttctggcagg ccaagatttt
    12061 actcgttcac ccattgtgca aggtggcaca accgctgatc atccattgat cgaagatacg
    12121 tatatcgaag ttgatctgga gaaccagcat atgtggtatt acaaagacgg aaaagtggcc
    12181 cttgaaacag atatcgtttc cggaaagcca actacaccta cacctgctgg agtcttctat
    12241 gtttggaata aagaagaaga tgcaacttta aaaggaacaa atgacgatgg aacaccttac
    12301 gagagtccgg taaactactg gatgccgatt gactggacag gtgttggtat ccacgattca
    12361 gactggcaac ctgaatacgg tggagactta tggaaaaccc gcggttcaca cggctgcatc
    12421 aacaccccac caagtgtaat gaaggaatta ttcgggatgg tcgaaaaagg aacgcctgta
    12481 ttggtcttct aacaaaaata atatataaaa agaaagcacc cgtacgattg gtacaggtgc
    12541 tttcttttta ttatctaaca atgtgctact tacatttcag cagcagtaaa gacttctgaa
    12601 acatcatcat cgtcttctag cttatctact agacgttcca attgctcttt ttgttcctca
    12661 tttaaatcaa tcgtcgtttg tggaatcatc gtcaattctg cttgcgccaa agtgaactct
    12721 ttttccaact catctcgtac agcagcaaaa tcttctggtg cagtataaat ctcgaatact
    12781 tcaggagaag tttgcagatc ttctgccccc gcctctaata catcctcaaa catcgtctct
    12841 tcatcaacag tcaacccatc gcgttcaata gcgatatatc ctttgcggtc aaacaagtaa
    12901 ctgactgaac ccgtttctcc cagtgatcca ccgtttcgag taaaagctac tcggacattt
    12961 gtcgctgtcc gattacggtt atctgtcaat gcatggacta agattcctac tccacccgga
    13021 ccatatcctt catacgtgat ttcatcgtaa ttcgtttcat ctacggcact cgtagccttt
    13081 ttaatcgccc gttccacatt gtcgttaggc atatttgcag cttttgcttt gtccacaaca
    13141 agccgcaaag ctggattcat agatggatca ggaccgcctg ctttagctgc catataaatt
    13201 tcacgagaaa gtttctgaaa gactttccct cgttttgcat cttgggcatt ttttcttcct
    13261 tggatattat gccatttact atgtcctgac atgatttttc ctcccttgtt cttttgagaa
    13321 tacacttcta ttctagtact atttcacaaa aaaacaaagc cacagccaat ctataaacgc
    13381 ttcttgaatg ccgcaaaaag cacacataat atcacagcag tcagagcacc gatcgaatcc
    13441 aacatgacat cttggaacat aggtgttcgt ccaccggtca tcatctgatg gaattcgtcc
    13501 aaacctgcgt accctgttgc tgaaagccaa gcaaggatcg ctgtcagcca ccaaattttt
    13561 agcttgggga aaacacctaa aaacaagctg cctcccaaaa taaaataggt accgaaatgt
    13621 gcgcccttcc ttaaaaagaa ctctaaaaat ttggcgtagc ccagatggtc gatactcacc
    13681 atacttccac cataattaaa acgtataccc gctaatgtgt ctttaaatgg ctgattaggc
    13741 agccagcttt ctaatcgcga aacttgtgat tgctgtccgt aagtttgaga agaactgtaa
    13801 aataagatta taacgactat cagtgcgatt actaaataaa tatttccgtt tttcatttgt
    13861 ttttttgaag aattcataag tcacctctgt attttatctt agactttttt tgaagaaaca
    13921 acaatcattt tcatagagac ttcttccata aaaaagtgct atacttaatt ttgtattaca
    13981 atatgaaggg agttttttca tgagttattc attaaattgg aatctagatt ctattttttc
    14041 cggaggcagt cattctgatg ctttgaacca acggatgaag caattggaag accaaatgaa
    14101 cgagtattat caccgcgtaa caaaatggtc acctagttct gacaatgcag agcagttaaa
    14161 cgctattctt caattgcaag aaacgattac gaatggtttt acccaatgca gcagctatat
    14221 tactgcattg ctttctgcca atgtcaatga ttcagatgca aaggtcttat caggcaagct
    14281 ttacgcaatg ctgcctcgtc tgcaatcagc cgaaactgta ttgtcaaaga aatttgcaga
    14341 aatatctgac aatgattgga atcagatgct ttctcaatct tcgtttgaaa caattgcttt
    14401 tcgtttaaac gagattcgtc gtgatggctc acaattatta tcagaagctg aagaaaacat
    14461 tataaatacg ctttctttag atggtctaaa tgcttggagc agtcattatg atacgattgt
    14521 agccagcatt tcgattccct tcgaacaaga tggacaagta gtcgaattat ctgctggaca
    14581 agccttcaat aagatgatgg gcgatccaga tccaaaagtc agagaaacac ttttcgcagc
    14641 atgggaagaa gcctggaaag aaaaggcacc cctcttcacg gatactttga atcatttaga
    14701 tggttttaga ctttctgact atgaacttca tggtgtcaca gattttctac aaaaaccatt
    14761 agagtacaat cggttgaaga aagaaacatt gtctgtcatg tgggatacaa tccagaaaaa
    14821 caaacaaccc cttgttaatt acttgactag aaaagctaat cttttcggta aagaaaaaat
    14881 ggaatggcaa gatcaagatg cacctattat tttaggcgat ttgaaagaaa aaacctttag
    14941 ttttgacgaa gcagcagcat ttattatcga aaactttaac aaattcagtc caaaaatggc
    15001 taaatttgcc caatctgctt ttgaaaagag ctggatcgaa gcagaagatc gtcctgggaa
    15061 acgtcctggt ggttattgta ctgagttacc agaaacaaaa gaatcaagga tctttatgac
    15121 ctacagtaat tctgttaatg aggttgcgac acttgctcat gaactaggac atgctttcca
    15181 tagcagcacg atgtgggatt taccttcttt aaatcgcgaa tatgccatga atgtcgcaga
    15241 aacagcaagt acattcgcag aactgatcgt agccgatgct acattaaaag tcgcaaaaac
    15301 aaaagaagaa aaaatcaatt tgctagatac aaaactgcaa aatgctttag ctatgttcat
    15361 gaatatccat tcccgattca tttttgaaaa tcgattctat gaagcacggc aagaaggatt
    15421 agtctcagaa gataagatca cagagatgat ggttgaggcg caaaaagaag gctatcaagg
    15481 agctttagct acttaccatc catatttctg ggctgcaaaa ttgcatttct ttattgatga
    15541 cgtaccgttt tataacttcc catacacatt tggctacctc tttagtttgg gcatctatgc
    15601 ttacgccaac caaaagggag caaattttga agaagaatat atccatttac tacgcgatac
    15661 tgcctcaatg acgacggaag atcttgcgaa gaaacatttg aatgtcgatc ttacccaacc
    15721 agatttttgg caagcaggta tcgacatggt gactgaagat atccaaacgt tcatggaatt
    15781 gacagaagaa tttatctgat ttctatttta tcacaataaa aaaagagtta gtccagaatg
    15841 ggctaactct ttttgattaa tcattcttac atgctgacag caaagtcagg ggcaaaccat
    15901 tgaactgaac caacttttac gctttcgcct ggttgaggag catggatata ttgtcctccg
    15961 cctaaagcaa tcgctacgtg gtaagttccg ccttgtgacc cccagaataa caagtctcca
    16021 gcttttgctt gagaaactga aatttttgtt ccagctgatt cttgaggaac tgtccaacca
    16081 ccgatgtcac gaccagttac ttgcatgtaa acatagcgtg tgaaccctga gcagtcaaag
    16141 ccacttggat ctttaccgcc ccaaacatat ggagtaccaa tatatttgta agcttctgct
    16201 acgattgctg caccattcac acttccagaa ggagctggag caggtgttac aggttttggt
    16261 tgttcaggtg ttggtgttgg cgttggagcc ggagtactac ttcctgtgcc gtttcctgta
    16321 tcaacacttt gatctgttga tggtgttgta ggcgcaggtg ttgttgattc ttctgttgtt
    16381 gaagattcag gcactgttgt tgattcttct gttgctgaag attcaggcac tgttgttgat
    16441 tcttctgttg ctgaagattc aggcgctgtt gttgattctt ctgttgctga agattcaggc
    16501 gctgttgatt cttctgttga agattcaggt gttgttgatt cttctgttgc tgaagattgt
    16561 gtagctgatg atgattcttc agtcattgaa gattgtgtag ctgatgattc ttctgttgct
    16621 gagctttgtg cagtagatga agcttgagta gcttgtgctt cagcctctgc ttgttcgcgt
    16681 gcttcttttt cagctttttc ttgtgcagct tgttgacgag cttgttctgc tagacgttgt
    16741 tgttcacgaa tacgagcttg ctctgcttca gcttctgctt tttgacggtt caagtcagct
    16801 ttcttgtctt cagcagttgc ttgttcagca gctaatgaag ttttcagtac gttcaaatca
    16861 gcttgtttag ataaaagatc gcctttttga gattccaaag cagcttggtt ttcagctaat
    16921 tcttttagtt tagcatcgtt ttcagctttt ttgtcttcta cagctttttt atcttgtttt
    16981 tgttgttcca tcaagtcgtt gtttgctttt accatcgttg tcattgcttg aacgcgtcca
    17041 atagcatctg ctaatgaatc agcattcaat acagcatcga tgtagtttga gcttgtgttg
    17101 cttacttgtg cttcgcgtgc ttgtttttgg attgtatctt cacgtttttc aatacgttct
    17161 tgtaaatctg caatgtcttt tactaattgt gcagattctt gacgcaatgt atcttgtttt
    17221 gctaataggt cttgtgcttg tgtgttgatt tcagaaactt ggctttctaa ggcatcgatt
    17281 tgtgattgtg catctgcttg ttgatttttc aagtctgcaa tcttttgatc ttgctgttgt
    17341 atctgagaat caaaatcgtc cgccgcggca gcgatcggtg atgctacggc agtcaaggtc
    17401 attgaacata ccattactgc tgatattaaa ctctttttca ctcttcattc ctccgactgg
    17461 cttaatttaa taaatatcta aatatattta agtctttctt aacgatataa aaattgtacc
    17521 atagcagtgt gtcaacgata taatagttat gttacaaaaa tgtttcaaaa caacttttaa
    17581 aaatgacaaa actgttcgtt tcagaaaatg atcatgaaaa agccgcgtta agtctagcat
    17641 ttcttaagat aacaacgatt ctttttcgct ttcgacctaa aaataaaaaa agaaattatt
    17701 catcactgaa taatttctta aagggaaaaa tgaataatac aaatataatc atgttcaaca
    17761 gcagtgtagg tcctaaaaac cttgtaataa aatacgtgtt atttactact gctaatttaa
    17821 aaatcaactg gaccaccatc gtaaacaatt cataacccgt gacaaagatg atcatactga
    17881 aaaattcagt aaagatgttg acatggataa cgtctttcat tacatacatc aaccatacaa
    17941 ttaaagggag agctactgca tagataccga taacgccaat atagtaagcg tcatagatag
    18001 ccccgagaac aatcgtggta atcagtaaat aacgtttttc aaacgccata ctgcaacata
    18061 ataatgccaa tatcagaaag tgggcatttg acatataggt gcctttcgac cactccccta
    18121 gcattcttgt taaatgacca tctatcaaca tcaaaaagaa caaaacgatt ggcagatagt
    18181 acttcattgt ttctttcttc aacatggact aatcctccgc tagccgttgg acaatcgtca
    18241 caactgacac gtcatacatt tctgcataag gtttcacata aacttcccga tccagcccat
    18301 aactatctgg tttcgtttta ataaccgtac cgatcggcaa gttagctgga gaattccctc
    18361 caagaccgga tgtctggaca acatcccctt ctttgatatc cgtatcacca gttaattggg
    18421 tcaccactaa ggcatggagc ttttcatcat agtttttaag caaaccaaac gcttcgccat
    18481 tagccgaaga tacccgtact ggaaaatgat tggagctttc attagaggat gacagtaatt
    18541 cgattttaga cgaagccgta ttgacctcga tcactcggcc aatcaatcct ttttgcgaca
    18601 tgactgccat atttgcttcg ataccgtctt tagaaccctt gtctacgatc agcatgtcct
    18661 gccaactgtc aggcgaacga gtaatgacat ttgccgttac tttttcgtaa ctagtcagcg
    18721 tctgattcaa ctcaagttct tttttcaacg cttcgatttc tctctcgtaa ttcttgtttt
    18781 gctgtgaaac gtcatcatag ctatcgattt tttcttttag ccgctcattt tcggcataag
    18841 ttgaaaaaag attatggaca gaatctactc catttcgtac ccaccgtact ggggcagaga
    18901 ttgttttatc aaccattgca acactgtcat taacgatgga ttggaagaaa ttgggctttc
    18961 cttcattcgc tcgctgcgca gcggtcacac ttagtatcgt caccacgatg ataacaataa
    19021 ccaaggtaat aataatattt ttattaggat tgaacttctt cacaaagaca cctcgaatta
    19081 attagactct tgtgttcaat tttatcacta aatgccgtga aacaaaaggt aagttgctcc
    19141 tcttaagctt ttttttagca aataatagat tctgtcattt ttagcctgaa agacaaattt
    19201 ttttatttgt ttatggatca atctttgctt tgaataactg caatcatacc atctgaaaaa
    19261 gaaaattctg cggtatctga aagaaattcg ttacttgcat aagatgtcgg atattctact
    19321 tctttatttt ccaatacata cttgctgcct tttaatgtaa gattcgagac gggagtcaag
    19381 cagcaataag ctaaatactt catatctggt atctgtctta ctatatgctc tccaggcaaa
    19441 taataagaca aataattttg acaatctctg ataataagct gatgggcaaa gggtttgaag
    19501 cgaggttcca tacctaacca gagattggct aaaagatgat ccaatcttcc gcccgtagct
    19561 ccaatcaaag tgacttccgc ttgcgggtaa cgttcaaatg tttttaagat acctaactgt
    19621 gtatctgtat cgtctttttc tgcaggggaa cgggtatact ctttggcttt tgcaaatact
    19681 tccttctgct cttcgttcga caatgaatca aaatcgccca ctgccagatc tactgggtag
    19741 cctgcttgaa gcaggtacag cgcaccacgg tctatcccga cataggcatc gtatttggac
    19801 agcagagact gcggccagtt ttgcggatta cctccagcag ctaataaaat tttcatttag
    19861 tctaatgcct cttttaatcg ttcgatttgt ccgactggat tttcggctcc atagatatat
    19921 gatcctgcaa cgaagacatc cgcccctgca tctttacaga tttttgctgt ttctggaacg
    19981 attccgccat ctacttcgat tgcataagtc caattgttct ctctacgtag atcagcgagt
    20041 tccgtaattt tttctactgt ttcttcaata aagctttgtc cgccgaaccc aggattgaca
    20101 gtcatgacta gtacttgatc agccaaaggc aaaacatgtt tgatcatgga gactggtgtt
    20161 cctggattga taactactcc agctttaact cctaagcttt ttgtcatttg aagtgcccga
    20221 tgaatatgag gagttgcttc gacgtggaca gtaatgatat ctgctccagc ttttgcaaat
    20281 gctggaatgt agttttctgg atcaacgatc atcagatgga cgtctaacgg taatttagta
    20341 acaggacgga ttgcctgaac aatatttggc cctaatgtga tatttgggac aaattggcca
    20401 tccattacat ctacgtggat gtaatctgcg cctccttttt ccactaactc aatgtctctt
    20461 tgtaaattgg caaaatctgc actcaaaatg gatggtgcta tcttcattct tgtttcctcc
    20521 tattttcgtt taccttcttt ttttgcatac attggttttc ttttttcgat ttcttgtaag
    20581 aattgcaaat aattgtcgta acgtgtctgt gcgataccac ctgtttctac tctatgtttt
    20641 acttcgcagc caggttcttt agcatgcatg cactctctaa atttgcaatg gacagaagct
    20701 tctacaaatt caggaaattg tctcggcagt tctcctgctt caattgtcaa aaagtcaatc
    20761 gagctaaatc ctggtgtatc tgctactaac ccgccataga taggaatcag ttccacatga
    20821 cgtgttgtat gccgtcctct tccaagtgca tcagaaatct ctcctgttgc taaattcagt
    20881 tccggtgata tttgattcag tagcgtcgat tttcctgctc cagactgacc cataaagacc
    20941 gatagccgtt ctgggaagta acggatcagt tctgtcgtat cacccgtatg attgggtaca
    21001 atcaccggat agcctatttt agtataggtt tgcttgatct cttcgatttt ttctgggtcg
    21061 tctgacaagt cggtctttgt caaaaaaata atcggttcga tcgactcgta ttccaaggta
    21121 actagaaagc ggtcaagtag attataagaa aatgcaggct ctacgatact tgtgacaatg
    21181 attccaagat caacatttgt gactggtggg cgaactaatt gattttttct cggcaagatt
    21241 tccagaagat agccgtctgt ttgattttca ctctcaaaga tcacctgatc tccgacaagc
    21301 ggcgtgattt tccgattccg gaaatttcct cttcctcttg tttgataggt ctctccgttt
    21361 gcgtatacat agtaaaaacc gctgattgct tttctgattt gtcctttgat aagtgccacc
    21421 tccgtttttg tttattttac catacgaaga ggaaagtagg tgttattcca tgcaaacttc
    21481 tcaaagtata tggcttgaaa attgatcaaa agagatagaa acacaaaaaa gagaatgcaa
    21541 caaatcaatg tcgcattccc ttctgatata ttttattcag cacttatttt gaactttctg
    21601 aggtgctagc tgatgaactt gtggagctgg aagattcttc ttttgaagag ttgcttgtgc
    21661 tactggagct acttgagctg ctagaactgt ctgaagagtc tttttcagaa ctagaagacg
    21721 ctggatctgg tcctttagag taaattacgt tcacaaccat tcctttggta attgtcgttc
    21781 ctgcccctgg tactgtacga atcactttat ctttatccac cgtgtttgaa tattcggaag
    21841 tctcatgccc gatatattct gcaccgacac tagctaaata actttgtgct tcgctttttg
    21901 tatagccgga gatatcattc aatttaggct ctgttccttg gcttacgaca aaattgatgt
    21961 aagtttcttc tgctacaact tcacccgagg ctggatcctg actgatgatc gaacctgatg
    22021 gtacagtgtc gctatagtca taagtagcac taatattatt gatattgaat ccttgtgtaa
    22081 caagccaatt acgaacagtt tcgtagtttt ctcctacatg actactcaaa gtcactgttt
    22141 ctggtccttt gctgactgtg aaagtaatcg tgtctgtttt aggatcgact tctgtatctt
    22201 ctgatggaga ttgcgaaatg atcattccgc tttctacctc tgaactgaat tcctcttctt
    22261 tttgaatcaa gtcagcagaa aaccctaatt ctttcaaact tgcttcggca tcttcataac
    22321 ttttaccact gtaatcatct aatttgattt ttttcgtgcc gctactaata tacagagtga
    22381 cttcgcgttt ttgcttgact gtcgttcccg ctgccgggtc tgtcttcact acattacctt
    22441 cttcgatttt gtcatctgca atttcttctg tttttgttgc tacttctaat ttcgcttttg
    22501 cgagtgtttc tctagcagaa gcttcagaaa gatcagaaac atcaggaaca acaacttctc
    22561 ctcgcccgcc agaagcaaag aatacagctg ttccgattcc taaaacagca agaatagcta
    22621 ataggatcag ccaccatttt ttctgtcctt tctttttagg atcaggcaga acttcttcct
    22681 cctctggcaa ggtttcgttt tctggcttgt cttttggcag aggcatagat ttgaatgaat
    22741 caggcattgg tgcatcttcc gtaaggggtg tgataacttt tgtttcatta tccatcacat
    22801 gaggctgcca tttttcttcg tttgcacgag ctggtgataa tactgtagaa agatcatcag
    22861 acatttcctc ggctgactta taacggtctg caggttcttt agccgttgcc cgtaaaatca
    22921 cgttttccag tgcttgtggc acatttggat caagtgcttt tatagatgga agatcatctt
    22981 gaaaatgttt taaagcaatt gttacagcag attcaccatc aaacggtacg ctacccgtca
    23041 acatctcata aaggatgatc cctaaagcat aaatatctga ctgcttcgtt gccatacttc
    23101 ctctggcctg ctcaggggac agataatgaa cagatcccag catcgtattc gtctgcgtga
    23161 tcgatgtttc agataaagcg atagcaatcc cgaaatccgt gatttttaca acaccttcat
    23221 tatcgatcag tacgttttgg ggtttcaaat ctcggtggat gatttgatgg ttatgagcta
    23281 aagaaatagc agaaaggatc tgctgcataa tatttactgc tgtttcataa ggaacaggat
    23341 aatgtgtttg gatatatcgt ttcaagtcca ttcctttgac gtattccatc accagatact
    23401 gcatgttgtc ttcttcacca acatcgtaaa cactaacaat gtttggatga acgagttctg
    23461 ttgctgctaa agcttctctc tgaaatcgtc gaatcgcagt ctggtcattt tgaaagtcaa
    23521 atcgtaagac tttaactgct acatcacgat ccaagattaa atcgtgcgcc aaaaatacat
    23581 ttgccatacc accgctaccg atatttccag tgatttgata acggccattt aattttttgc
    23641 ccaactcgat catacactgt cctccttata ttcgataaga agaacggtga tattatccgc
    23701 tccgcctgct tcattggcac gatcaatcag ctcagttact ttatgagaca atgggccttc
    23761 ttgattgatg atcgttccta tttcttcttc agacagcatg ttcgtcaagc catctgaaca
    23821 taacagcagt cgatctttta gttgccaaag atgagtagaa acatctactt cgactgttcc
    23881 aggcatgccg acagaacgtg ttaaaatatt tttccttgga tggttcactg ccatttcttc
    23941 gctgatttct cctgatttga ctagttcatt gactaaagaa tgatcctccg tcaattgcat
    24001 gattttatct tcacgaatca aataagctct gctatcccca acattggcaa tcgtgaacca
    24061 gtcatctgac agagcggccg ctacgattgt tgttcccata ccgttatatt ccggctgttc
    24121 ttgtccacgc tgataaatca gcatattttc ttcttgaatc gattgaatga accattgggc
    24181 gattttttca tcgtcaacag cttcttgttt ttcccaagct tcgcctagat tcgttaccgc
    24241 catctgactg gcaatgtctc cggcacgatg accacccatc ccgtctgcta atacagcaag
    24301 cttgagacct gaacggctgg taaatacact ggcatagtct tgatttgtgt tacgtcgtct
    24361 tcctacatct gattgatatt caatctgcat tttttcacct cattactttt tgcgcaaaca
    24421 acaaatgaaa aatccatctg tgtaaaactg atgcggatac aaagtcaaca ttttctcttc
    24481 gattgatgat tgtagcatag gattggtcaa aacatcaatc ctctcaaaat cagggtgtct
    24541 ttccaagaaa gcagaaacaa cgtcttggtt ttcttctttt aagatcgtac aagtgctata
    24601 agtcagaata ccggaagact ttaaagttgg tgcgcagctt tctaaaattt ccaattggat
    24661 ttttggtaaa ttcgctaatt catccggctg ttttttataa cgaatatctg gttttcgccg
    24721 aagcagccca agacctgaac aaggcgcgtc tactaaaatc cgatcaaact gctctgcttc
    24781 gaactgttta ctgacttctc tagcatccat cttttgagcg tagacagcag aagagacacc
    24841 tagacgtgct gcattatctt caatgagttt gactttgtgt tcgtgtacat ctaaagcgac
    24901 cacttctcca ccgcatgacg gatctaaaaa agaagcaata tgcgtcgtct tacctccggg
    24961 tgctgcacag gcatctaaga catgatgttc aggctcgatt tgcatagacg gcgctacaag
    25021 catcgaactt tcatcttgga tcgtcatgac gccatcgtgg aacaatgagc tagaagctaa
    25081 atgccctttt ttcgctacta taccgtatgg actgacttgg cttttttcag ctgtgatctc
    25141 ttcttcttgc aaaacagtga tggcctgatc tctggaaagt cggcgtgtat ctactcgggc
    25201 actagcatgg ctaggctgat atagcgataa accgagcttt tcagtttctt cgtatccgat
    25261 ttgatccacc agtcgtttcg tcatccatag aggcaggctg attttgacag aaagtctttc
    25321 gataggatca gcaatgcctt gcagatctgg tgctccttga cgctgataat tcctcaagac
    25381 accatttaca aatttccctg tcccaggatt tccttttact tttgcgattt cgaccgcctc
    25441 gtttaaaata gcatgtgcag ggactttatc taaaaattct aattggtata cagaaagata
    25501 aagaagcgtc ttgacccagt catccacttt ttttgcttta gcgatcaatg gtgcaacgta
    25561 aaattccaat agtcgctgat ggctaatcgt accatagatc aattctgtaa ataaacccac
    25621 atctttttga tttagctcgc tttgtttgat catctcattc aacaaaagat tcgagtaggc
    25681 gccacccttg ttcactcgct ctaatgtcag taatgccata tatcgtactg attttttcat
    25741 ttttttaggg atttttttca ttatttcacc tgctgaccaa tctgaacttg ctgaccgctt
    25801 ccgttaagga aatcatgtac ggcctgtttt cctttaccag ctggctgtaa ttcattgatg
    25861 gctaagacgg tctgtttacc gcaagcaatc catagatttt ttttgtcttt ccggatgatc
    25921 gtaccaggtg ctttgtctgt agtttctgcc aatacttcaa ccgataataa tttccagcgt
    25981 atgccctcat aggtcgtgaa tgcaattggc caaggacgca ttccgcgtac ttggttgtca
    26041 atttcttctg cagttttatg ccaatcgatt gcttcttgtt cccgtgtgat gtttggcgaa
    26101 aaagttgctt ctgactcgtc ttgaggtact ggtttttgtc catccaaaat attgggcaat
    26161 gtttctagca atagctttct tcctaaaagg ctaagctttt caaacatcgt ccctacatca
    26221 tcttgtttag tgattggtat gctttcttgc gcatagattc cgcctgcatc catttttttg
    26281 atcatttcca tgatcgttac accagtttct ttctccccat tcatgatggc ataatgaaca
    26341 ggcgcacctc cccgatattt aggaagtaaa gaagcatgga cattgattgc ccctaatttt
    26401 ggtacttgca ataacttttc aggcaagaat tggccaaatg ccgcagtgac gatcacatca
    26461 ggcaccaatt caatgatttc ttccatttct ggcgaaccgg agattttctc tggctgcagc
    26521 acacggatac cgtgcttcag agccgcttct tttaccggtg tcggcgtaat tactcgtttc
    26581 cgcccaactg gacggtcggg ctgtgttacc actgcctgga tctcgtaacc tgattctatc
    26641 agcccttcaa gaatcggcac agaaaaagca ggtgtgccca taaatactag cttagtcatc
    26701 aagatgttcc tccatatatt cttcaagttc ttcttccggg atctgctgta tcattttctc
    26761 aatgaacaag ataccgtcta agtgatcgat ttcatgctgg aaggcacggg ctaaatagcc
    26821 aaaagccgtc acttctatct cttctccgtc tcgatcataa taacgcacgg tcacttcatc
    26881 tgctcgcttg accgttccat agacatgcgg aatactgaga caaccttcta cgtcaaggct
    26941 ttcacctttt gcttcgatga tttctgggtt aatcagttca aatttctcgc cttcatctac
    27001 ttcgatcaca gcaattctct tgttttgtcc tacttgagga gcagcgatcc ctattccgtc
    27061 attcgcaatc attgtctcat ataaattgtc taataaagcg attgtctcgt ccgtgattac
    27121 atcgattggc tgagcagttc tttttaattt atcattaggg tggatcaata taggatagcg
    27181 cataatttgg ccctttctta aataaagttt aatggttcgt tgtcaattgc cacaaaaaga
    27241 ccttgtcgct gttctttctg gcttccattc aaaatttcat ccaaaacatg aggaagattc
    27301 ggttcatgtt tatatttgat gattagttga taataatatc ggtttttcac tctcaaaatc
    27361 atgctgggcg ttggccctaa tagcaagctt tgagggctta acacttcttt tagctgattg
    27421 gcaatctgaa agattttctt tgccgctact tgttcttcag gatggctggc ggttattttc
    27481 acagtaaagt aataaggagg atagcctcca cgatgccgta catgcatttc ctgctgataa
    27541 aactgctcat aattttgttt tttagctaat tctatggcgt aatgttgagg attgaatgtt
    27601 tggatcacga cttcgcctgc tttttccgct cgccctgctc gtcctgaaac ttgggtaagc
    27661 aattggaatg tatgctcact agaacgaaaa tccggtaaat tcagggaggt atcagcattc
    27721 aacaccccaa ctaatgtcac ttctggaaag tccaaacctt tagcaatcat ttgtgtccct
    27781 aataatatat ctgcttcttt tgcaccgaat ttttgtaaaa tctgttcgtg tgcccctttt
    27841 cttcgagtgg tatctacatc catacgcaaa atcctagctt gcggataaag ttctcggagt
    27901 tcctcttcta ctttttgagt acctgttcca tagtagcgga ttttattgcc gccacaatta
    27961 ggacagcgat tgggaatagg ttcttcatgt ccgcaataat ggcagcgcat tgaacgtgta
    28021 tccatatgca gtgtaagaga gatatcgcaa ttaggacatg gaagaacaaa tccacagtcc
    28081 ctacacatca caaaagacga atatcctcga cgattgagca gtaggacagt ttgttctttt
    28141 ttgtctaatc gattttgtat tttttcctgc aaattagcag aaaaagtaga ggtccgatga
    28201 ttctgaaact cttcacgcat atctacgatc tcaatagctg gaagctgtgc agctgctttg
    28261 gctcggtggt ttaactgcaa taactgataa acgccttttt gagctcgtgc tcttgattct
    28321 aaagagggcg tcgcgcttcc tagaactatt gggcaatgat gatatttact gcgccaaatt
    28381 gctagatctc tcgcatgata acgcggtgtc tcatcttgtt tgtaacttga ttcatgttct
    28441 tcatcgataa tgatcagccc gatatttttc agcggggcaa aaatagcaga tcgtgcgcct
    28501 actactactt gcgcttcttc ccgctcaatc ttgcgccatt catcatattt ttcaccttgg
    28561 gaaagtccgc tatgtaagac cgcaactgat tctccgaagc gtcctttgaa tcgctcgacc
    28621 atttgcggag tcaatgcgat ttcaggcact aacataatag ccgttttatt ttctgacaaa
    28681 acatcagcga ttgcctgaag atatacttct gttttcccgc tgccagtgat tccctccagc
    28741 aaaaagacct gatctttttg ctgctgacct gctgtgatga tttgttcgac agcatttttt
    28801 tgttctgcat tcagttccaa tgccgtcgtt tggtcaaata ctcggtcttt ataagggtcg
    28861 cgataacgtt ctgattctat aaactccagc cagccctttt cttttccttg attcaagaca
    28921 gcagtactga ttcctttatt tttgaaataa gcaattggtt cttcttggct cagttcagat
    28981 aaacacaaaa gaagttcttt ttgtttcttg gcattttgcc gtaattttgc ccattcttct
    29041 tcgatttgct tttcagttaa caaagaacgg accaaacgaa tcgtttttac tttatttcgt
    29101 gtatggactt catatttgat atctactaat tgctcttggc gccagcgaat caattgagaa
    29161 aggctgcctt cttcttctgc ttccttccag gaaatcactt ctcgctgatc aaacaatacc
    29221 tgaacttctg gtgtgtctgc caaaggatag atcaatttat catattctgc tttcatcaca
    29281 ctcggaagca tcgtttgtag acaagtgatt ttgaatgcgt aagtaatttt tttcatataa
    29341 tcagctaatg aaagtaattc ttcattgacc accggagcaa ggtctagcaa acgaataact
    29401 gccttcaaag atggattagc tgattcttct gatttttgga tagccataac aaatccttgg
    29461 acatgacgat tgccattacc aaaagggact tctactcgca tcccgacttg caaagctgtt
    29521 tccatttcag agggaaccaa ataagtgaat ggctgatcgg tctgcatagt aggtacgtcc
    29581 acaatgactt cagctgttaa tgtcatggtt cgtttcctcc tttttttgtc ttttcttatc
    29641 aagcgacaaa aagttccttg cctattttac tagagatcga tggatttgtc ttatcttctg
    29701 ccaaatccta acaaaaaatt aacagcagat aagaaaaaaa agaactgtgt caaaaagttc
    29761 agacacagcc ttttctacca gacaataagt ggcgaatgtt tgaaaaggaa acagctgggt
    29821 ttgcttttct ttttagatgt tcgctctatt tttctttatt acattttttg ctcttcacgg
    29881 atacgtgatt ccaattcgtg ttgttcacgc tctttttgtg cttgacgttc ctcttcttcc
    29941 atcttcaaac gttcacgttt taattcagga tgtggatcat tgactacatt tcctgcatcg
    30001 atttcttcta aagcttgacc gacgcttttt actgaatcaa aagaatctag agtaggctgt
    30061 gcacttgcat ccaattcatg cgcgcgttta cttgctaaaa tcactagaga atattttgaa
    30121 tttacacgat ctaatagtga gtcaattgaa ggttttaaca tcataagact acatctcctt
    30181 taacatttta atatatttac cgattacgcg atctactcga aaatgttcac tggcaataat
    30241 gtttttgatt cgttcaactg ctagcggcac ttcatcattt acaacagcat agtcgtaaag
    30301 agccatcatt tcgatttctt cttttgcaac tttcattcgt tcttcgatta cttcatctgc
    30361 atctgttcct cggcctacga tccgtgattt taattccgct aaatctggcg gagtcaaaaa
    30421 gatgaataca ccatcgggca ctttttcttt gacttgtttt gctccttgga cttcaatctc
    30481 caagaaaaca tcttttcctt catccaatgt cttgttgata tatgacaaag gagttccata
    30541 ataattcccg acatattctg cgtattccag catctctcca gcttcgatca ttgattcaaa
    30601 ctcttctttg cttcggaaga aataatcaac gccttcgacc tccccttcac gcattttacg
    30661 ggtagtcatt gagatcgaat actgaaaatc attatcctca ctctcaaaaa ttgcttttcg
    30721 tactgtgcct tttcctacac cggatggacc agacaacacg attaataatc cacgctctga
    30781 catgacgacg tcctttcaac tagtcttttt gtacttatcg tttattttgc actttttttc
    30841 tgcaactttc aaggttatct ttaattatag ctagaaattg gtaaaaaagc taccctgtac
    30901 caagaggtcg taaaaaagct gtcctgcatc aagtaataag aacagccaaa cataaacagc
    30961 ttctcatgtt tttcgtttgg ttggggttat tatcgcagat gcaagctttt gaacgccgtg
    31021 tataagaggt cgtgaagaag ccgattagtt tcgagtatga agaaaaattt tgtgaaaata
    31081 gtttctcaca tttttgcata aatttggctt aatac
//
LOCUS       DACIRY010000024        30820 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.024, whole genome shotgun sequence.
ACCESSION   DACIRY010000024 DACIRY010000000
VERSION     DACIRY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.024"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(351..905)
                     /locus_tag="IYE22_000890"
     CDS             complement(351..905)
                     /locus_tag="IYE22_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ9506121.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1031..2960)
                     /locus_tag="IYE22_000891"
                     /pseudo
     CDS             complement(1031..2960)
                     /locus_tag="IYE22_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3266..3982)
                     /locus_tag="IYE22_000892"
     CDS             complement(3266..3982)
                     /locus_tag="IYE22_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ9506122.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3983..5806)
                     /gene="glpO"
                     /locus_tag="IYE22_000893"
     CDS             complement(3983..5806)
                     /gene="glpO"
                     /locus_tag="IYE22_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ9506123.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5820..7316)
                     /gene="glpK"
                     /locus_tag="IYE22_000894"
     CDS             complement(5820..7316)
                     /gene="glpK"
                     /locus_tag="IYE22_000894"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ9506124.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7452..8912)
                     /locus_tag="IYE22_000895"
     CDS             complement(7452..8912)
                     /locus_tag="IYE22_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9506125.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8902..10197)
                     /locus_tag="IYE22_000896"
     CDS             complement(8902..10197)
                     /locus_tag="IYE22_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ9506126.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10529..11206)
                     /locus_tag="IYE22_000897"
     CDS             complement(10529..11206)
                     /locus_tag="IYE22_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ9506127.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11236..12327)
                     /locus_tag="IYE22_000898"
     CDS             complement(11236..12327)
                     /locus_tag="IYE22_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ9506128.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12428..12862)
                     /locus_tag="IYE22_000899"
     CDS             complement(12428..12862)
                     /locus_tag="IYE22_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9506129.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13446..14903)
                     /gene="ascB"
                     /locus_tag="IYE22_000900"
     CDS             complement(13446..14903)
                     /gene="ascB"
                     /locus_tag="IYE22_000900"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ9506130.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14900..16795)
                     /locus_tag="IYE22_000901"
     CDS             complement(14900..16795)
                     /locus_tag="IYE22_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9506131.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16853..18628)
                     /locus_tag="IYE22_000902"
     CDS             complement(16853..18628)
                     /locus_tag="IYE22_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9506132.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18678..20174)
                     /locus_tag="IYE22_000903"
     CDS             complement(18678..20174)
                     /locus_tag="IYE22_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9506133.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLYYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20368..21990)
                     /locus_tag="IYE22_000904"
     CDS             complement(20368..21990)
                     /locus_tag="IYE22_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ9506134.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22318..23631)
                     /locus_tag="IYE22_000905"
     CDS             complement(22318..23631)
                     /locus_tag="IYE22_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ9506135.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23876..24100)
                     /locus_tag="IYE22_000906"
     CDS             complement(23876..24100)
                     /locus_tag="IYE22_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506136.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24120..24488)
                     /locus_tag="IYE22_000907"
     CDS             complement(24120..24488)
                     /locus_tag="IYE22_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ9506137.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24684..25394)
                     /locus_tag="IYE22_000908"
     CDS             complement(24684..25394)
                     /locus_tag="IYE22_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506138.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25412..25909)
                     /locus_tag="IYE22_000909"
     CDS             complement(25412..25909)
                     /locus_tag="IYE22_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9506139.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25946..26398)
                     /locus_tag="IYE22_000910"
     CDS             complement(25946..26398)
                     /locus_tag="IYE22_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9506140.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26533..27138)
                     /locus_tag="IYE22_000911"
     CDS             complement(26533..27138)
                     /locus_tag="IYE22_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ9506141.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27588..28208)
                     /locus_tag="IYE22_000912"
     CDS             complement(27588..28208)
                     /locus_tag="IYE22_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9506142.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28210..29079)
                     /locus_tag="IYE22_000913"
     CDS             complement(28210..29079)
                     /locus_tag="IYE22_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ9506143.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29102..29509)
                     /locus_tag="IYE22_000914"
     CDS             complement(29102..29509)
                     /locus_tag="IYE22_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ9506144.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29528..30817)
                     /locus_tag="IYE22_000915"
     CDS             complement(29528..30817)
                     /locus_tag="IYE22_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9506145.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8745 a         6688 c         5090 g        10297 t
ORIGIN      
        1 tgaaaatgga tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
       61 aataccgttt aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagaaacaa
      121 acggaaaaat agctgctcat attttgccgt tttttgagct aataccgaag gtcagctgat
      181 tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca caagaaataa
      241 tttcaaaaac aacttctcct gtttttggtt cggttggctt attatcgaga agtagctact
      301 gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt ttattttctt
      361 tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag accgtaagtg
      421 atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag cagcaaaagg
      481 atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc tccacttaat
      541 ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc ttttatcaag
      601 aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa aatcagaaaa
      661 agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt tacctgatta
      721 tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac aatgaccgct
      781 cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag caaaaaaata
      841 cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat
      901 ttcattttat cgccctacct cctcagcact cttttctttt tattttagct tacacgggga
      961 ataaaaccaa tgatttcact gtttttattt ttctttacag acactgatat tatgagctaa
     1021 tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca
     1081 tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt
     1141 tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg tacagatctt
     1201 ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt ttgtctaaat
     1261 atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt tggtattcat
     1321 tgacaaatat ccgatcacct tttgcaaaga gctgacgttc ttttttattc tccttcctat
     1381 ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct ttttccgcca
     1441 agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga gcaatccaca
     1501 aagcttggct ttctcctgtt tcttccaacc cgcaactgat atttaggacg aagtgcttcc
     1561 cgatcgaaag ccatagcagc cggcacaaaa tcttcatgat ctccggcgaa tttcttgatt
     1621 tctccataat gggtcgtggc aatgatcaat gccccttgct catacataga ttccatgatt
     1681 gcgatcgcta gtgcagcccc ttcatttggc tctgttccac tgccaatctc atctaaaagt
     1741 accaacgtat tcctttttat ctttttcaaa atagcagcga tgttttgcat atgaccagaa
     1801 aatgtactca acgcattttc taaattttgg tggtcaccga catcgacgaa aatctcatca
     1861 aagacagcaa gctctgtccc ttcttttgcc ggaacttgca taccgaacat cgccatcaaa
     1921 gtcaataaac cgactgtctt caacacgact gttttgcctc cggcatttgc tccggtgata
     1981 atcaagcctc gatagtcttt accgatttca acatccaatg gtaccgcatg ctcaggtaaa
     2041 aatggatgtc tgccttgctt gataatgata tgttccgatt tattgattct aggtgtgctt
     2101 ccattgattt ctcggctaaa ttttgcccga gcaaagatga tatctaacac cgtgatcgtt
     2161 tcgatcaata aatcgatcac ttcttcgttt tctgcgatag caccagtcaa tgccgccagg
     2221 acttgatatt cttcactgat ttcttcagct tttaacaact gatagtgttc attcaattta
     2281 gaaatcacag ttggttcaat aaaaaccgtc gttcttttat ttgattcatc gataatcgtt
     2341 ccatctattt tatttttata gctggctttg ataggaatcg tatagtattc gcctttttga
     2401 acaatcattg cttcttggat catttctttg tttttcggat gacgcaagaa tttcagtaat
     2461 tttgactgga tttctttttc gattagttgt aattgtttgc gtactttacg caaattacgt
     2521 gacgcgtcgt cactgacttt attatttttt attttctggt caatcaactc ttctacattt
     2581 atcaaatcag gaagatgttt gctataagca aacaacaagg gtgtctggta ttgattctta
     2641 tcaaaaaact ttgtgatcat ccgactgctt ctaagaaaat ccgcatattc gatcaaatcg
     2701 gttggctgca gaatcaaccc ttttttgact tgttctgtca gtgcatcgat cctcggcaac
     2761 cccataaatg gaacatgctg actgctttct aagatcaagc gtgcttctct tgtttcttct
     2821 tgacgatcca agacagtttg taggtttgtc gagaccgtca tatcgctgat tcgttttttg
     2881 ctatagtttc caattgctct tgcttggatt tcctttttga tccgatcaaa ttgtaattga
     2941 ctaatcattt cgttgtccat attctctcct tttcttgaag agatacctgt cccaaaataa
     3001 ataagggcct agagataaat aaaaggatag aacacagctt aaaaaagtca tgtcctatcc
     3061 ttattagaat acaaagatat tgttttcctg acaggtaccg tttcataagc acaccaaata
     3121 aactttgttt attggctatt cgacttactg attagatacc agctacactc acaaaacata
     3181 ccccattctt ctaggtctta tttattttcc acttaactat agcaagacac attttggttg
     3241 tcaaacttta gtatgtttta tagatttaca ccattaatga atagacacct accgcgataa
     3301 ttgctccgat cattggacct gcgattggta cccatgaata tgcccaatct gaagttcctt
     3361 tattagctat tggcaatacc gcatgtgcta atcgaggacc aaagtcccga gcagggttga
     3421 ttgcatatcc tgttggccct cctaatgaaa gaccgatggc taagatcaat ccacctacag
     3481 cgaatacgtt cgttccaggt gcaaattcgt tttgtccaaa agctagcaac ccaagaacta
     3541 atacaaatgt accaattaat tctgtgatca catttgctgg ataattccgt atagcaggac
     3601 ctgttgcaaa cgttcctaaa atcgcgcttt catctttcgt tgcattccaa tgtggcaaat
     3661 aagaaagcca aacaagaatg gcccctgcaa aagctcctaa aacttgcgca atgatgaaag
     3721 gaacgacaag attccaacta attgcccctg tcatcgccat agctagagag actgcaggat
     3781 tcaaatgagc tgggcccatg aatccagaaa tatagacagc cattgttaca gcgagtcccc
     3841 aaccaaatgc gatgacaatc cagccagaag cctgtgcttt acttttgttt aaattcacag
     3901 ccgcacacac accatctcca agcaaaacta ggatcagtgt cccaataaac tcccccatga
     3961 tttgagtcat atcactgttc atttatttct ccccctttag ttcccgcaga tctgattctg
     4021 caatcaatgc ttctaaacgt ttagtctgtt gcgccttctc ctcttctgtc cattcaaaat
     4081 atccagcaat tgcatcgatc acaggttgct tgatctcatc aagctgatct cgttcaaaca
     4141 acagatgatt tgtacgacga atgagataat cgcctggggc caaaaccatt tcttcttcta
     4201 aaccataacg taaccgagct gactcagcga gactcaaacc aggataaggt gccatttctt
     4261 ttgctaattc aaagatacgt cgggcattag tcccgtaaaa atcagcgata taagtagcat
     4321 cttcttcctc taaaccggct gctactcctt cttttgctaa ttctgtcact gtttcttcta
     4381 atttagttgg atcgaagttt ccacctgaaa tctgatattt tttagagtcg atttctttcg
     4441 tctctattcc gtattcttct tttaataatt ggcgtatcaa tcgcaaagct ccttctgcca
     4501 tcttacggta atcggtgatt ttcccaccag ataaagtcag caaaccatct ggttctcttt
     4561 ctaaagaact acctctggaa atcgtagaag gtgctttttc atcacggctg ttttccaaat
     4621 ggttcaacac gtcttctact tcagcacgag aaactttgtt ttccttatat tcacttactg
     4681 tatcgacaac tttattgaag ctcttgtctg aaatcgatcc attatctcca ccattgtaat
     4741 cagaaccaga attaccaatc aacagtggac gaagtcctgc ccagcttgct tcgatatcat
     4801 tcaatgtgat atttgcttct ggatagcgat gattgatcac atccaataga taatctacat
     4861 cttcttgtgt gactttcgga tccgtaaaat ctccttggta atccgtatct gtcgtaccaa
     4921 agtaagtctt gttttctctt ggaatagcaa aaaccatccg cccatcttgc tttcctgtat
     4981 cgaagtatgt gggttgcggt acaggcagtt ttttcgcatc tacaactaaa tggatccctt
     5041 tagttggacg cattttagga gagactggac gcgtaaaatt caagtttcgt actttatcta
     5101 cccaaggacc actcgtattg atcactaatt ttgctttgat ttcgatcact tcatctgtca
     5161 gcaggtcacg agctttgacg ccaacgattt gatcgccatc atataaaaag ccaaccgctt
     5221 tcattttact tactagataa gccccatctt cggcagcctt tttgatatta tcaatcacta
     5281 aacgggcatc attgttgcgg aaatctagat agacacccgc gccttttagc ccttcttttt
     5341 tcaaaaacgg ttctctttcc aatacttctt ctggtgtcag cgtataattc tcatatttgg
     5401 ttcctgtcac atttgccagt ttgtcataaa ggtccattgc tactttgacc gagaacatat
     5461 tgaaggttgt tgctccttcg ccctcataga ttggcaaaag cattggatct ggttttggga
     5521 tgtgtggggc aatactttgt acgactgcac gttcaccaac tgtgtcagct actacttcca
     5581 catcaaatgt cttcagataa cgaataccgc catgaacaag tttggtcgag cgagaggaag
     5641 tcccttctgc aaaatcctgc atttcgatca atcctgtttt gattcctgat gctgctgact
     5701 ggactgccac accggcacca gtgattccgc caccgatgat caacagatcc aattcttctt
     5761 gctgcatttt ttgaatgcta tcttgtcttg tcttgtttga aaacatatgg atttcctcct
     5821 tattcaaatg cttgggtagc ttttactgct tttttccaac cgttgtaaag tttttctcgg
     5881 cgctcttcct ccatttgaac ttcaaatagc ttgccagctt cataaaattc gcggatctcg
     5941 tctgtatctt tccaataacc gacagctaat cctgctaaaa atgctgcccc tagcgccgtt
     6001 gtttccaagt tttctgctcg ttggatcggt acattcaaga tatctgtttg gaattgcatc
     6061 agatattcat tgtttgctgc accgccatct actttcaata caggaatctt gattccagtg
     6121 tcatcttgca tcgtatcgat gatatctctc acttgataag caatcgactg taaagtagct
     6181 ttaatgatat cttcttttgt cgtaccacgt gtcaagccaa acatagcccc tcgtgctgcc
     6241 tgatcccaat aaggagcacc tagcccaacg aatgcaggaa caacataaac ctcatcttcg
     6301 cttgtagagc gtttcgctgc gtcttctgaa tcgctagctt tttgcaacat ttgaagtcca
     6361 tctctcagcc attgaatcga agaaccagcc acaaaaatac ttccttctaa agcatagtaa
     6421 acctttccat ttatgccgta accaattgtc gtcaataaat tatttttgga taattgcggt
     6481 ttttcaccgg tattcatgac aataaatgaa cctgtaccat aagtattctt gaccatgcct
     6541 ggttcgaatg ccatttgtcc gaataatgcg gcttgctggt ctcctgccat ccctgaaatt
     6601 ggtgcttcgc ttccataaaa atgataacct tgcgtataac cataaacttc tgagttagaa
     6661 gtgacttttg gtaacatcac tcgaggaata tttagtaaat ctaaaatctc ctgatcccaa
     6721 tctagctgat ggatattgaa caacatcgtc cgactggcat tcgagtaatc agtaacatgt
     6781 gcttgtccat cggtcagttt ccatactaac caagaatcga tcgttccaaa catcaattcg
     6841 cctttttcag ctcgttcttg cgcgccttct acatgatcaa gaatccagcg tatcttcgtt
     6901 gctgagaaat aggcatcgat cactaaccct gttttttcgt ggaaaaattc ttgatagcct
     6961 tcatttttta agttatctgc aattccagct gactgtctgg attgccaaac aactgcatga
     7021 tagattggac gtcctgtatc tttttcccat attactgttg tttcacgctg gttcgttatg
     7081 ccaatcccag caatctgatt tggtttgatc cctgattcga tgaacgctcc ggcaataact
     7141 gactgtacag aattccagat ttcatttgca tcatgttcca cccaactttc ttgcgggaaa
     7201 tattgcgtga actctttttg agaactgccg atgtgtcttc ctttcttgtc aaagataatt
     7261 gctcgtgagc tagttgtacc ttggtcaatc gccatgatat acgttgattc tgtcattgtg
     7321 attccctcca ttatatgcaa acgctttctt taactactct tatcatacta ctgttttaaa
     7381 aaaagaaact gtcaaaattt tattcttttt ttcttttttt gacagttttt tattatgaag
     7441 ctatttttta atcaattctt ttcttttttc agcgtctcta ttcttctttt caatcgaata
     7501 atgtcgtaag gtgacgcaaa ttctgacaga taatattcac actgtttttt gtttctctgg
     7561 caaaatgtga tcaccaaatc atattcttta ccttcttcat aacttccaat ctcaataccg
     7621 ccgattcccc tcagttctct gatcagatat tgttgaaaag caatacgata aatcggcaga
     7681 ctttctaaat cgattccaat cactactggt tttgcaatat aaaaatcgat catcaaaaga
     7741 acattggcat actggatcat caggtaacta agctcactat cttcagctct tttcctcggc
     7801 aattgctgca ccgctgtttc ctgtaatttc gccagtaact gtgttaagga atgcccgagc
     7861 attttctgct gctgttccaa aagccgatca cgctcataga cttcgatctt tccaacaaag
     7921 aaaaaccatt cattgttgat ctgggaaatt tggtagccta ctgccttttc ttctttgatt
     7981 ttcaaacgac gaggacgata atgccataac atcgtttccc gaatgtaagt atctaaaaca
     8041 gcggttggta actttttgct ccgtgtcaaa tcatactgat aaaatatttc ttcgtctact
     8101 atcgaaaaag aaacaaaaaa gctgtaaaac atcattgttt cgtatacatt cagttctgct
     8161 gctgttcgac taagatgcat cacgatgatt ggatcgattg cttgataaag ccggtccgac
     8221 tgatagagta atttcttttc tttcaaagtt gcgtacgtac ttttttcatt caatagtctt
     8281 tttttcgtaa tcccgagcca gcaagcaatc tgtgcttctg ctgatgccgt aaacgttgtt
     8341 tctaatacac ggtccaatcc tagaatgaac ggtctttttt ctggtgtatt ttgcaaaaaa
     8401 agcggtcgct gatcttccgg gatatattgg aatagttcat aataaaaata tcgtatctgt
     8461 agctcttctc cgtataattg accgtttttg atttgtaatc caaattcctc taagagctgg
     8521 ttcaattctt tgatctttcg aaaaacagaa gactcactga tattaaacgc tgtcgctaat
     8581 cgaacgatgg atacttgctg atgttctaat aaatagacga gcatcttaaa cttcaagctt
     8641 tcctgcaata aaaacaatag aattttttcc aaacttacat cttcgtccaa cacaagcagc
     8701 gcctgatact catttcgctg tacttctact tttttttcca tcattcggcc aatcaattca
     8761 atatcttcca aatattgatc aaacgaggct tttgacagtt ggacatgctc tctcaaacca
     8821 ttgatagagg ctgttccccc ttgcagaaca aggtttcgca aaatcaggat ctgtaactgc
     8881 tcttttcttt ctaataatgc ttcaatcttc ataggcagta ttcgccacct ttgaccacac
     8941 catatctggc acacggtatt ctggctggaa aaatagctca tgttcttcta actgttcaaa
     9001 cgtccatcct tcttctactg caattgtcag ttggtcaaga aggtggaaca tctctttttt
     9061 gctttggagc tgtgctccaa gaatcttttt ttcagaatca aatagaaatt tgagtttaac
     9121 tttgctttga tttattgctg taagcttgta gtctacgtat cctgtttcaa ttttttgggg
     9181 ataaaaaatg ctctcttctt ctgtcaaacc tacgcttcct aagaaatagc caaaccactg
     9241 attgccgact ggacgatagg ttccctgatc ctttgtctgt gagccactaa ttgtcttgct
     9301 gacatttcgt gctgtacgga cagcgttatt tactagagaa acatacattt tttcatttgt
     9361 tggacgaaaa gttacttgga tcgcatcgcc aatcgcataa acgtcttttt gagacgtttg
     9421 caagtagtca tctacccaga tcgttccatc atcattcaat gttaattgtt cttcccacat
     9481 ctgattgtcg ggacgagaat gattagccag caaaacaaga tcatagttct cctttctttt
     9541 ttccgtctct acaatagcgg tttcattttc ggtcaaagac acaacttgtt cttctaaaaa
     9601 aagtgaaagc ccttgatttt tcatttcctt tactaacggt tctgtcattt caggatctag
     9661 ataacgaaaa agaattgact ttcggctttc gtaaagatgg atttgttttt ttcttgatgc
     9721 caacccttca gctatttcga ttcctgcttg tcctgcacca ataattgcaa ttttctttgc
     9781 attcatgatc ttttgctgaa gagccgttac gttttgtatc tttttaactg ggtgaataag
     9841 cgttgattct gcagaaatat ttcgaaactt ttgatttgaa ccagtcgcta gaatcaaacg
     9901 atcaaaatcc aatttttttc ctcctgctaa aacgactttt ttttctttca atcctattac
     9961 tgtcttttct gtgtaaacat ggattcgata gcggtcagtc agttcttctt tcgtgatcca
    10021 agaaagttgt ttttcactgg taacctttcc ttttaatatc aaattgaacc cgctgggaat
    10081 ccatcctaac ttcgattgct tttcaatcaa atatatttca cttgttggca tcaattggcg
    10141 aagataaata gccgcatgaa tccctgcaaa cgacccgccg acaattacaa tgttcatctg
    10201 cattcctctt tcttataagg tgttctactg ctaacttctc aatcagaaaa cacagtattc
    10261 ataactcgct ttcaacctta tgatacatag cgatgcagca gaatcaaccc tctccatcat
    10321 accgtgttct aggtctgctt ctcaataatg tactagtgga cctaattgaa gagtatcatt
    10381 tctgaagaga tgtttcaaaa aatgtactct tataaaagaa aaaaactcgc cttttacgta
    10441 cctctgtcca aataggaaac agaaaagaac gaaaagacga gttttagcgt gtgtaatgta
    10501 atagttagaa tagacacatc atttattttt aattctttaa tgcttcaaca actgcttcgc
    10561 ctaattgatg agcagattga ggattttgcc ctgtaagaag ccgcccatca atttgaacca
    10621 cttctgaaaa agctgctcca gactgatagt ttgctccttt ttctttcaaa gcatcttctg
    10681 ctaagaatgg tacttcattc gttgtaccat ttgcttcctc ttcttcatta ctgaacccag
    10741 tcactgtacg tccagctaaa atagattttc cagactcatc tgtaactggc aacaatccta
    10801 caacaccgtg acaaacagct gagaccagcc cttcatttcg atagatttgt gaagcgactt
    10861 tcccaagatc tattgcttct ggaaaatccc acattgttcc gtggccacca gcataataaa
    10921 ccgctgcata atcatttgca tccacatcat caggacgtaa ggtatttccc aaggcccgat
    10981 ttctgaaatc ttcatcttca taataggccc aatcgatttc atccatattc aaaacacttt
    11041 gcggttctaa aggtacatat ccgcctttag gactgacaaa atctgcatcg atcccatttt
    11101 ttactaatac atcataaaaa tgagtcagtt cacttaacca caaacctgta ggacgctcga
    11161 tcttttcgta cttttctaca tttgttaata cgatcaatac tttcattttc ccatcctctt
    11221 tctataaaaa ttgcgttatt gataacgtgc tttcaagaaa ttatataatg ggcttggcaa
    11281 ataaagctct ttatatgctt cttcaaaagg tttcgttaca aattcatcct cacggttttc
    11341 caataatttc tctgcccagt caggatcgac gatcatttgc tgtccaattg ctgcaagttc
    11401 tgcatatttc aatacctgct ctacatcttc gcgattgcgg atcccaccga caccaactaa
    11461 agggatcttt ccttgaatcg tgttatggac cacttcaaga atcgatttat cgttgtattt
    11521 atcagaacga gctattcgat catacatatt taatgaaaca tgcagataat ctagttttga
    11581 ctcacgtaat cgttcaagaa gccagatcgt gtcttcaaaa cggatacctg gtgtttcata
    11641 ttcttctggt gagaaacgat accctacgat gaatggtttt ttagcatatt gttcaaccgc
    11701 tgagaaaacc gttgttaata attcttcaat aaaacgataa cgattttcca agctaccgcc
    11761 ccattgatcc tctcgtctat tcgaatgcgg agagaaaaat tgttggatca aatacgtatt
    11821 tgctccatgc aactcgattc catcaaatcc tgattcaatc gctctacgtg tggctttgcc
    11881 aaatgcttgg attgtttcaa cgatttcttc ttcactcatt gctcgtggtg tttccgcatc
    11941 aggacgtttt gctgcaactg cacttgcaga tacaggctgt tctccagata aagttgctcg
    12001 acttgtcatt cgtccaccgt ggaaaatctg aataatcgct tttgcgcctt ctccttggat
    12061 tgctttcgct agctcactta atcgaggaat catttcatca tgagcgatgc tgagttcacc
    12121 tggccagcct tttccgatat cttggacatt tgcagcacca gtaataacag caccaagacc
    12181 tttagaacgt tgtttataat aagtgatctc atcttcggtg atcactccat tgaaaaagct
    12241 gagttgtgtc gtcataggcg acagcatcaa tcggttcttc aattgaactc ctgaaggtaa
    12301 agtaattgat tgatcaaatt gtgacattgt cattcctccc ataattcgct ctgattaacc
    12361 tatcgagaat catctatcat taaaccactc gattagttgt acatgcaact aatttgctta
    12421 aaaaatttta ttttttttgt aacttatctg ttgcatcatt cattgtctgg gctaattctt
    12481 tacttgcttc atttcccaaa actgctttgt atgactgatg aagttcttcc cagctaccat
    12541 cgacttcctt agcaaattgg cgtccttgtt ctgtaagata aactaatgac aagcgacctt
    12601 cgctaactgt tttgacgtat cctttatatt ctaatttttc aacaaaacga gcaatcgttg
    12661 atggggcgga atagagcatt tgtgcaatat ctttttgtgg tattccatct ttttctttta
    12721 atatcaataa aataaaacca taagttggtg ccagacctgt ccttctaaac gcttcatcag
    12781 ctaatttatt caacatgcga tctagctttt tcacgctaaa gaaaagacat tcatctaacg
    12841 ctttttcaat tggcttttcc atttcatccc ctccattggc gtacatatca tagtatgatt
    12901 tttatttttt gtcaaaaaac acggaataat gtccgcctcc tctgtctaga tttgaaaaaa
    12961 agaagtagcc atcttcaaag aatttcggct tgttttcgac aaaactcctt ctggaacacc
    13021 gtgaatcaga ggttgtgaaa aagtcgttta gttttgagta tgaagaaaaa ttttgtgaaa
    13081 atggctttcc acatttttgc ataaatttgg cttaataccg aaaaactggc ttttgaacac
    13141 cgtttatatc agaggttgtg aaaaagccgt cctgcatcaa gtaataagaa cagccaaaca
    13201 aaaatagttc ctcatatttt gtcgtttttt gagctaatgc cgatggtcag ctgattgtac
    13261 accgtttatt gagaggtcgt gagcctgccg ttgaactccg agtcacaagg agcaatttca
    13321 aaaaacagct tctcatgttt tatgactatg gtgacttgtg atcgaggagt tggcaggaga
    13381 atactgttta ttcgtaaaga aggggctgtg acaagacttt tgtcacagtc ccttatttcc
    13441 ttttattata ggatttcgcc atttgattgg atgacttgtc tgtaccaatc aaatgatttt
    13501 ttctttaacc gtttctttgt gccattccct tcgtcatctt gatcaacata gatatatcca
    13561 taacgttttg acatttgtga agtagaagcg ctgacgatat caatgcagcc ccacgacgta
    13621 taaccaaata aatctactcc atctttgata gcttccttca tctgctcaat atgctgcttc
    13681 aaatagtcaa tgcggtaatc gtcttgaacg gttccatctt cttctacttt gtctatagct
    13741 ccgagtccgt tttcagctat gaacagtggt ttttgatacc tatcatataa ttgattcaaa
    13801 gcaattctca aacccttagg atcaatctgc cagccccatt cacttgatgg aagatacgga
    13861 tttttaattc ctccagttat taagtttcct tggacccctt cccaattttc tggatgagca
    13921 ctgactgcac tagacatgta gtagctaaaa gaaacaaaat caacagggta ggctgctaag
    13981 atttcttcat cacctacttc tatttttaag ttgacacctt tttcttcaaa cattcgttgc
    14041 gtatacgctg gataatatcc tcttgcttga acatcactaa agaacaaaga agttctttgc
    14101 tgctcaattg ttttttgaat atcatccgga tcacaagaat acggaacaag aagctgatag
    14161 ctgatcatac aaccgacttg tgaacctttg ataatttcat gacaagcttt tgtagctaaa
    14221 gcacttgcta taaactgatg gtgtgcacct tggtatttcg cttgttccaa atggggatgg
    14281 ttttcttcaa ttactcctac gctaacaaaa gggtgatgtt ttacacagtt gatctcattg
    14341 aaagtcatcc aatattttac tttattttta tagcgtttga atactgtttc agcgaaacga
    14401 acgtaaaact gaataagctt tcgattaggc catccaccat attctgtaac caaatataac
    14461 ggcatttcat aatgggaaag agaaacgact ggctcaatcc catattttag acattcgtca
    14521 aacacttgat cataaaaaaa taatcccgct tcattgggtg tttcttcatc accatgagga
    14581 aaaattcttg tccatgcgat agacatccga tagcatttaa aacccatttc agcaaataaa
    14641 cggatgtctt ctttaaagtg atgatagaaa tcatttccgt gtcttttagg ataataatac
    14701 ttgctgttgg gatcctgcgc cgcttttact tgctgatgcg tcatcatatg ccattgatct
    14761 gtccattttt ctttaggtaa atcgctgtcg aatgtataca catcagacac agataatcct
    14821 ttttcatcta catcccatgc accttcacat tgattggccg ctgtcgctcc gccccataaa
    14881 aatccttctg gaaatttttt catatcgatc cccctaaact tgctagtttt cttgtttgat
    14941 tatttttctt atgactagta atgatatttt tgaatctact actatttgta acaataacca
    15001 ttgtggtcgg atcatatccc tctttgatta attcttgcaa atctacttcc atcaataaat
    15061 cgcctttacg tacttttttt ccatcagata ctgcactttg aaagtatttg ccatttaaat
    15121 tcactgtatc aataccgata tggatcagta tctctgctcc ttcttcactg actaccccaa
    15181 ttgcatgttt cgttggaaaa actgttgaaa cttccccgtt tacagaactg tagatttttc
    15241 cgtccgttgg aatgatcgca aacccttctc ctaaagatcc actagcaaat gttggatcgt
    15301 ttacttcaga taaatcaatt atttcacctg ctgcaggagt acttaacact atatcctttg
    15361 aggattcttc ttcgatttca tccttgtagc cgaaaagaaa agttagcata caggaactga
    15421 tcatcgctaa gaaataacta gcaactagcc aaacaaatcc ttcaccaata taaatcggga
    15481 aagacaaaat acttcttgta gcaactgcca aagcaccggc tcctgcggca ccagctactc
    15541 caccaaatat agctccacat atacaagcaa tgtaaaatgg tcgtttatat ttaagagtca
    15601 caccataaac agcaggttca gtgattccaa ataatgctgt gataaatgca gataaagata
    15661 aactttttac tttcttattt ttcgttttca aaaatacccc aagtactgct cctgcttgcg
    15721 cagctacggc agtcatacat gctggaccta gcgtatcacg accgtagaaa gaaagattgt
    15781 tggtcattac tggtacaagt ccccaatgta acccaaaaat cactaacact tgccaaaatc
    15841 cgccgataac agcaccagat aaaacagggc taaagttata aacagctaaa tatccagctg
    15901 caatcagctg acttgcataa tctgcaattg gacctacaac taataaaata attgggacca
    15961 tcacgatgat acaaatgaat ggtgtcaata aatttcgaat actttcgtgc aagcttttgc
    16021 gaatcttagg ctcaatataa gaaagaaccc atacagctaa aattgctgga ataaccgtac
    16081 ttgtatacct tgctagaatc acgggcatcc ctagaaatcg caaggttatg ctatcactat
    16141 atgcactgac aatcgtagga taaacgagag ctgctgcaat aaccatagcg atgaaacgat
    16201 ctgtattgaa cttttttgca gcagtatatg ccaaaaatat aggcaagaaa tagaaaaatg
    16261 catcagctgc tgcattcaaa atgatataag ttcctgattc agtagttaat aaattcaaac
    16321 tcgtacataa ggcgagtact cctttgatca aaccacttcc tgccaagacg cctaaagtcg
    16381 gggcaaaaac agcggcgatc atgtctataa aagagtttaa aatgcctttt ttctcagttc
    16441 ctctctttcc actagatgca tcatcttcta gtttcatttg tttggataat acgtcataca
    16501 cttcatttac tgcatttcct actacgactt gaaacattcc tccagcttcg actacagtga
    16561 tcacaccttc tagttgctct accttctcct tatctgcctt cttattatct tttagggtaa
    16621 agcgtaacct tgttgcacaa tggtaaacat tgctgacatt ttcttcgcct ccgacaaatc
    16681 ggacaatatc tttcgctaat tgtgaatagt ccatttttac ttcccccttg ttaattaatg
    16741 ccgtagcaat ccttcttaca agataagtat agttcttaga aaacggtttc tccattcaat
    16801 tagtacccct tctaatagcg aacgaaactg tacaatagtg tgtatggttt acttagccga
    16861 gcaaatcact cactgcttga aagaaaacaa gtatatcgtt tctcttatag tcaaaggaga
    16921 caaaaaaaac acgcttgatc ttcctttgct gatgtataag tggtatctca agctgatact
    16981 ctgtgatttt tgtttctact tggcttttga taagacagca caacaaagtc tcggcattta
    17041 acggaagcat aactaggtca gaactaggag agattagttg ctgaatggaa tctatctttt
    17101 tatggatttt tatttgattt tcttctttgt agtaatgatc aagcagtttt gttttctttt
    17161 tttcttcaac tatggtcagc ttttcacgca aaatctgata aatctcccaa gattcaaact
    17221 catctagaac actaggtaga taaatcaaat catcccactc aaaaataagc tcttgctcag
    17281 tagtcaaaat tgcatcatat tccgttattt tgtttgattg atttttgaaa agatacactg
    17341 gttctatccg gcattctttg atccacatgt tcatactttg ttgaatctgt ttttttagtg
    17401 tattcaaaat actaattggc tggttactaa caatcagtat ctccccttgt tttcgaaaag
    17461 gattcaacgc ttcggcaaga tacaaaacaa aataagcgct ttcagctaaa ttgggacgta
    17521 attgaaaatg tattgggaag aaacgccgaa ttaaataaat ttctaaagga taacgggaga
    17581 tgatcttttg tacaaaatga ttcatggtat tatgtccggc atccatcctc gttttcattt
    17641 gtttgatatg ggttagcaaa aattctgttt catggaacaa taacgaaaca ggaagatcta
    17701 aaaaacgaat aatcgtttgc tcaaatgctc ttagattctt ctctatttct ttgttttttt
    17761 catcattaat catcaaataa tttaactcta atagtcgatt ttcaattgct tgtttttctt
    17821 gattggaaaa agaatagggt agttctgatg ataacttttt tgtgagggtc tctaccatcg
    17881 gaataaataa tgtatacctg tttgtttttt ctaaaagttc ttctcttcga agaatggtca
    17941 aaacgagata ccgagaaaat cgtatgaagt cctctcctga aacaatataa ttatatgata
    18001 taagaacctc ttttaatgtc ttttggacag tcggcatgat attggaaaaa attgtactga
    18061 tttgttgagg aaaccttgcg tgttggatca ccgtttctgt tccaacaaga gataagaaaa
    18121 ttctttttga attttcttca gcaatgattt ttagtccttt tgtcgacgaa acttccagtt
    18181 ctaatttagg attgcgctcg atccaacgca aaatcgtttt tatttgtaat gatacggctg
    18241 tttttgaaag aaagaaaatg tctgctatct tttgcatact gacataccct ttttgaaaca
    18301 ataagtacgt cacaatagtt gaagctcgga aatccatgct ggaatagatt ttatgtttcg
    18361 tcacctcttg tatgatttgc tgtttagttt cagattcaat tgattctaaa aaataacctg
    18421 aacgtttatt tgactggata atgattttct tctcgtagat agcaagcata tttttgattt
    18481 cagtttggat cgttttcttg ttggtaccta aagcttcagc taattcttcg cttgttatcc
    18541 aatgattctt atttcttaat actacaagaa tttggagttg tcgtttattt aagtccattt
    18601 gcttacacct cctctttgtt ataaacatac aggaaaaacc tctcaaatga caatagtcac
    18661 ttgcagaggt ttaaaaatca tttgaagaaa gatctatatt caaggttttt accattagac
    18721 tggataattt gtttataata ttcaaaacta tcttttttca atctcctaaa gcttccgctc
    18781 ccatcatttt gtctatccac ataaataaag ccatatcgct tcctcatttc ccctgttcct
    18841 gccgatacga tatcgattgg tccccaccat aagtaaccaa tgatttggca accatctttt
    18901 attgcctcag caatttcctg aagatgtatt tttagatact cacaacgttt tggatcgtgg
    18961 attcgattca attcatctgg tgcttcatct agtccaatgc cattttcaac tatgaataat
    19021 ggtaattcat aacggtctgt caattcattc atgacataac gaaaccctag aggatcaatt
    19081 ggccaactcc aaggttcggg agatttttcc ttcaaaaagg gattatcgaa accatttgct
    19141 ccgccagtat ctacgcgcat ttctgcttct tttttataga cagcgctacg ataataacta
    19201 aatgcaatat aatctgccgt gttagcttgc aagcactgtt cgtctccatc taacattact
    19261 ggtgcacagt cttcttcctt ccatgtcctg taaatataag ctggataatg ccctcggcac
    19321 atgacatcgg agaaaaacca tgaattgcgt ttaaattgca gtgaaccaaa tacattctct
    19381 ggatcacagt catatgcata agtcgctaat gcagaaaagc tacacataat acccatctta
    19441 gcatttggat cgattttctt taataattga gtagcacggg cgttcgctag aaacatatga
    19501 tgacaggctt gataaatcac tttttttgtt agtgtagcaa tgggttcaag tttatctcta
    19561 gcagggaatg taaccaatcc agcagctgca taaggcatac gaaaagcatt attgatctca
    19621 ttaaaggtca tccaatattt cactttccct tggtagcgtt caaagacggt ctgcgcataa
    19681 aattcaaaaa agtcgattaa ttttcgattt tgccaccccc catactctcc taccaatgct
    19741 aagggggttt cataatgact aagagtaatt actggctcga tatcatatcg atttaatgta
    19801 ttgattagtc tgtcataaaa tacgagacca gcctcatttg gcttctgttc gtctcctctt
    19861 ggaaatattc gtgcccatga gatgcttgtt cgaaaagctt ttaaacctaa ctccttcagt
    19921 aaacgaatat cctcttcaaa tcgatgataa aaatcaatcg cttcatgaga aagataatag
    19981 agtgattcgt ctaaagcaat ctcccaaatc tttttttctt cattccactg aatactagga
    20041 tgctggttca tgatcccgat aagcgtatct gtcacattag gtaatttgcc gtcttccaac
    20101 catgcacctt cacattgatt agcagctact gctccgcccc ataaaaaatt ttctggaaat
    20161 atttcctgat ccataactat ctctcctcgc tatcaacaat ttcttacttg aagtatagct
    20221 cgattcatca aaacaagata ttctaactat ttcggcagaa gagaagaaaa aaattgaatg
    20281 ctactcttca tttttaatat ttatatataa caaatacata agacgatccc cgaaagaaaa
    20341 cactcgttaa ccatctaagc aaacgattta ttttacttcc aacacaaatg cttcataagg
    20401 tcgtaaatca actttgccgt ctggtaactg tttcagagga taatttgaca caataaccct
    20461 taaagcttca cgtggaatag tgaacgtctg cttttcactg gacatattac aaataacgag
    20521 ccattgctgg tcttcatata tgcgagaata ggcaaagaca tgctcaggcg tcttttccac
    20581 taattcatag tctccccaga cgatgatgtc atgttttcta cgcaaatcga tcaatttctt
    20641 gtacatataa aaaacagaat cttgatctgc taacgcttct tctgcattga tttctttata
    20701 atttggattg actcgaagcc atggaattcc agtagtaaac ccagcatgtt cacgggcgtt
    20761 ccattgcata ggcgtcctag cattgtctct tcctttcgcc ttgattgatt ccaagatttc
    20821 ctcttttgta aatccagaag aaatcctttc gttatacata ttgattgtct caatatcttt
    20881 tgcttccgcg atatcatcga ttgggcaatt cgtcatgcct aactcttctc cttgatagat
    20941 ataaggggtg cctttcatca tatggagcaa gatagcaaac atcttggcac tttcttttcg
    21001 atacacatcg tcatttcccc aacgagaaac aattcgcgga agatcgtgat tattccaaaa
    21061 taaactattc catccttcat cacctaaaga tgtttgccat ttggataata cttttttcag
    21121 ttcagagata ctcaagggtt tcaaatccca tttgtctttg ccttcttgct gatctaatcc
    21181 tatgtgttcg aattggaaga ccatggataa ttcatttctt ttaggattgg aataaagttt
    21241 agcgatttcg gtagttgctc cccatgtttc gccaacagtc atcagatttt tgtcaccaaa
    21301 agttgcttga ttcatttcct gaagatactc atgcagttta ggtccattac ttgtgatttc
    21361 tttatccggt tctttgccaa tcatatcgat cacgtccata cgaaagcccc caataccttt
    21421 atcgatccag aaattcatca tttgataaat ctcttggcga acctgtttat ttttccaatt
    21481 caaatcaggt tgacgcttgc tatacaagtg cagaaaatat tgccccgttt tttcatccaa
    21541 ctgccaagca gaaccactga atattgagcg caatctatta ggctcttttc cattgaccgg
    21601 atcacgccaa atataatagt cccgataagg attttcttta tttttacggg cctcaatgaa
    21661 ccacggatgt tcatcagaag tatgattcac caccagatcc atgatgatac ggatatttct
    21721 tttctttgct tcttcaatca gttcttccat ttcttccatc gtaccgaatt ctgccatgat
    21781 tgcttgataa ttactgatat catacccatt gtcatcattc ggagactcgt atactggaga
    21841 aagccaaatt gcatcaatac ctaaatacgc cagataatcc aacctggaaa taatcccccg
    21901 caaatctcca ataccatctt gatttgaatc ttgaaaactt cttggataga tttgataaac
    21961 aactgaatgg tgccaccatt tttgttccat gattttcctc ctataaatgg atgtttctat
    22021 tttttgctta atcatttatt ttagtctctc tcactaaaaa ttgtaacgct ttcaaatagc
    22081 tattgcacct tgttatcggt aacagaataa aacaaaaaaa cagctgagga aattccctca
    22141 actgtttttt gaaaggattg atttatgcag ggaagaatgc tgatttcaca acttctttat
    22201 aaacggtgtt cactcagctg tcccttggta ttagctcaaa aaacggcaaa atataagaaa
    22261 ctattttttc gtttttttct ctaatactca ggccagaacg ctgatttcac aacctcttta
    22321 tttcacattc aatatccaca tgaatcccac aaatacaaag aacagaccat acatgattgg
    22381 agatacttct tttcctcttt tagctgctaa catggtaatt ggatagaaga taaaacctaa
    22441 tgcaatccca tctgaaatac tataagtcaa aggcatgccg atcaagatca agaatgaagg
    22501 gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc
    22561 taccataatc aatgctggtg cagttacttg tgaagtaaca actgatagta atggagaaaa
    22621 gaacaagcct aacaagaaga aaatccctgt agtaatagct gtgatacctg aacgtcctcc
    22681 aacggcgata ccagctgatg attctacaaa tgcaccaaca ggagaagtac caaataagga
    22741 acccgcaagc atagctgttg aatcagaagc taaagctttc cctacacgag gcatcttgtt
    22801 gtctttcatg aaacctgctt ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa
    22861 agtcacaagc aagaatgtca gcactacgac ccacatttga agagaattga tgtcccctac
    22921 atgttttaat gcaactaaaa atgttggttt caaacttggt gctgcggcaa caatttcaga
    22981 aggcattgga atcaaacctg tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa
    23041 gatgccacct ggtacacgcc gaacaagcaa gatagcagtt acgactaatc caaaaatcgt
    23101 caaccaagta gaaccaacgt tgagtgatcc taatcctact aatgtagatt cattcgaaac
    23161 gatgatccca ccttcgctca gaccaaggaa agcgataaat agaccgatac cgccagaaat
    23221 cgcatatttc aaatctgctg ggatcgcatc aatgatcatt tcccgcagct tgaagattgt
    23281 gatcaaaata aaaatcaaag aagcaacgaa tacgccggct aatgctgttt cccatggaat
    23341 ccccattcct aaacaaacag aataagcaaa aaaagcattg atcccaagag caggtgccgt
    23401 tccaattgga taacgggcta atacccccat taaaacacag ccgatcgcac tggctaatgc
    23461 agtcgcagta aacactgctc cttcatccat tccagaagca cctaatacag ttggatttac
    23521 aaataagata taagccattg agataaatgt tgtaaatcct gccagtacct cacgttttac
    23581 cgtggtgttc aatttttcta tttcgaagta ggaaagtatt ttttccttca tcttccatcc
    23641 ccctaaatcc gaacattata aataaagtcc gttaaaattt cgatttatta ataaaacaac
    23701 gtgttttatc atatactaaa acaaaacata agtaaacaaa aaattcggat tttaagcaaa
    23761 atccgaacgt tccatctgat taaatccctt tatctcaaag ttcatgaaca ttcactttgg
    23821 attacaagaa tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat
    23881 agtgtgattt gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga
    23941 caggattcag attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg
    24001 tcttcttttt tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc
    24061 gatacttcaa ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt
    24121 caatcagacg actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg
    24181 aaagtcaaga tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata
    24241 gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa
    24301 aacttctgca caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt
    24361 acaatactct taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt
    24421 tctacaaaga atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt
    24481 tgtatcatca tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc
    24541 attatatcac gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat
    24601 agtttctagg attgctttcc tccacaaact ttttttacaa aaaaaaaacg gcaatgaatg
    24661 ccgctcttct tatttcgatt cttttataat tctgcttctg attcatcttt ggttagtgtg
    24721 tgtctaacac tagttacgcc aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc
    24781 tgtgcgccac gtgccgcttg ttcagcattg atggattcaa tgaccagcac cgctgcttgt
    24841 gtttcttccg tcagcatcgt tctttgggct tcacgccagt tgagacagcg acagatcgct
    24901 ccttgttcat catagtagat gatttcttcc ggaagtgccg gagcatcgga atctgcacct
    24961 aaaggcagga atgattcgcc accttgggct tttcctaaat gcagatttcc tgcgatcttt
    25021 tcgatatctt ctccgccgca aggtacgcca aaagacaagg aaatactatt atagatatct
    25081 acaagcggat tgattgggaa aaattcccgt tcttgatttg cacgtttcag taaagcttca
    25141 atagaagaac gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg ccactgagca
    25201 atgacttcgt tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc
    25261 tcattcaata attgatgata cgtttcttca ctttcttttg ttacgtggtt atcgatacca
    25321 tttacggata agatttcgat ggttgcttca ggaaatactt cccagaaatt ggtatccact
    25381 attacttttt tcatctgttc acacctcttg attagaataa ttgcttgatg aatagatagt
    25441 ctgtttgggg atcatctccc atataaaaaa catgttcgct gtatttgata aatccttttt
    25501 tacgataaaa agcgagcgca acatcgttgt gttcccaaac accgagccaa atggctgttt
    25561 tttgcttttc ttttgctttt tcaatagctg tatctagtaa aaatgtaccg ataccttgat
    25621 gtttaaattt ttttcgtaca tagattcgct cgatttctaa aaaatgatca gccatttcct
    25681 cagattgact cgttcctgta tttagtttca ggtaacccgc aggctcttga tcgatactca
    25741 ctaggaaaaa atcggtttct ttatctttta gctcttctgt aagtttttct agtgcataag
    25801 cttgtgtcaa gtaatcagct aaatgctccg gtgaattatc ttttccaaat gtatctgtaa
    25861 atgtttcgat actaatttgt tgtagtaagt ctacatctga aggcgtcatt tttcgaattt
    25921 cgtatttatt cacggcttct tcctttcaat agatcctttt attgcctttt ttgacaaaat
    25981 tccaatcttc gctaacattc ttgcatactt tatttaaaag atattccaat tgttttgctt
    26041 cttcgtcaga aagaccattt aatgcgactg cattggagta gttgttttct cgaatgatga
    26101 aaggatagat ttctgctcct tttttagtcg gatagagttt cttgattttt ttattttctt
    26161 tatcttccaa acgttcgatc atcccattgg attctaattt cttgattgct cgggcagtcg
    26221 ttgtacgatc tactttgatc atttctgcta gtttttcttg gatgattcca ggattttcgc
    26281 aaatacgtac taaataaaga tactgtcctc ttgttaaatc gacttctttg aattcgatat
    26341 tgctaataga atctagcgct cgggcaatca ttccgatgtc tcgaaggatt tcagccattt
    26401 catcccacct ttattttgtt tgtaagacta gttcacctct ttttgttgca aatgcaacta
    26461 ttttcaataa aaaaatcaag ttctttttta ataactagta aagtatattc ttttcttctt
    26521 ttacgaaaaa gctcaatcac aatagatctg gtttttgttt tctaaaataa gtaactgttt
    26581 cttcgttgtt cgcttttctg atagctttgg caactcttct atactaaaaa atcccatgtt
    26641 ggaagtttca ttattctcaa taaacttggc ctcttcaccg tggatcgcac aagcaaaaac
    26701 aagtttataa tactgaaaaa gctgtggaat atctttttgt ttattcgtat caaaaactgc
    26761 tcgcaactga gtagtttcta ctgttaatcc tgtttcttca tagacttctt tacgcacatt
    26821 ttcttcagga gacaatccta tttcagcaaa tccaccagga agtgaccatt ctttcgtccg
    26881 tttatcttcg attagtagga ccttgccatc ttttttgata taggcacgaa catctacttt
    26941 tggtgttgga tgtccttctc caatcgttgt gagcgctttt atttcttcga aagtttcttt
    27001 tcctatttca ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac gctctttatc
    27061 aaatacatct tttccataat atagaccagc ttgagaaagt gcgagtagtc ttttgtattg
    27121 agccaacttc ttgttcattc tcttcccttc ttttttagca aatgtatgac tatgtaatca
    27181 agtatagaag actggtttaa ttacggcaaa taaatataat gaaatctgca ataaataaaa
    27241 gaatgttaaa aaactcgaga acaaaagaca ttcgacttct ctggggctgt gaataaatgg
    27301 cgttcaaaag cttgcatctg cggtaataag ccccaccaaa cgaaaaatat gacgagctat
    27361 tcttgtctgg ctgttcttat taattgaggc aggacagctt tttcacaacc tccccactaa
    27421 acggtgttct cctaccaact cctcggtcac aagtcacaat attcatcaaa catgagaagc
    27481 tgtttttttg aaattgctcc ttgtgactcg gagctgaacg acaggctcac gacctctcgg
    27541 taaacagcgt tcaaaagcca atttttcgat attaagccat ttctttgtta cttattgtca
    27601 ttgatttctt tttctaattt atccaatagt ggagtgtaat ccacttgagt atagtctgtt
    27661 acatcaaatt catatagtgt tcctaactgg aatttgtcat attttcggtc ggcgatgatc
    27721 tttttgaatg cttctttcgt aatatgattg gtccctttcg ttcgatcttc caatacgtca
    27781 ccatagtgat aatgatccat gatatagctc aaatgacgat ctgattcacg atcacgtttg
    27841 taccgacgtt gccataacac ttcaaagtct gctgttaggc gaatcgtgat cacctgatat
    27901 ccgtatctat ctgctaattc ttggagtcgt gctttttgct tatcactaaa aggatactcg
    27961 gacacaatca ctttctttcc tgcttccata tacaaatcaa gtgctttgta atagaaagga
    28021 taaactttcg tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc gatggattct
    28081 gcatagtatt ccttgatttc atcgagcgtg atggcataca tatctggaaa tcgttcccgt
    28141 agtttattca taagatatgt ttttcctgta ccaggactac cagccagaag aatgaaatac
    28201 tttttcatct catttatttc ctaagttaat tggactttcc aagttcataa acagtccaat
    28261 cttatgcttg acaatttctt ttactgcttc atttgctgga atcacattat ggcgaagaga
    28321 cttgttgatt tctgtttcac taaaggtttc tttagctact ttcgtccagt tgactaaaag
    28381 ctccgttcca acattgaatt tgcggatccc gttattgatc aattcaggat aatcttcttc
    28441 tttcacacct gttcctccat ggataactag ggggacatca actactgcat cgatttcttt
    28501 cagcaaggga atgtttactt ctgtttttga tttgaattgt ccatgattcg taccgatagc
    28561 aatagctaat gcatccacgc ctgtagcttc tacaaatttc actgcgtctt ccggtcttgt
    28621 atataccttg tcattttcag cgacatggat tccttcttct gtaccgccga ttgttccaag
    28681 ttctccttct acggaaacac cgtgcgcatg ggcatattct actactgctt ttgtcttcag
    28741 gatattttct ttgaaaggca gatgggaccc atcatacatg acagaagtat atccgctgtc
    28801 gattgcttct ttgatatcat caaagtctcg ggcgtggtct aagtgcaaga ctgcatcaac
    28861 catttcttct tctgcaatag ctgtcattac attgactaac acatgcatgc cgatatattt
    28921 agcggtatcg acacttgttt gaataatgat cggcgaaccc atttcttttg ctgcgcctag
    28981 catagcagga agcatttcta ggttatgcgt attaaaagct ccaactgtaa aattcaactc
    29041 ttctgctgtt tttgttactt ctttcaatgt tgtatacatc ttttattgcc accttatctt
    29101 tttatttgtt tcctttgatc gttacttgtt ttgcaatttg tcgcatctca tccattttgc
    29161 ccatgtagta atcgatccct tgatcatcac cagcacgagc agcatctgca cgcttctggt
    29221 tatagaggct catcagtttt ttatacccat ctccgatcgt ctgtttattt tccaaactct
    29281 tttctaaata ttggatggct tgatcgttca catacaattc agaaaattta agtccaactt
    29341 gttcataaag tttcgctaat ttatgcttat cttgggtagt ggaaatctct tgtttcaatt
    29401 gtgtaatctc tgattgaatc ttttcggtcg cttctggcga tagggctttt tcttcttttg
    29461 ccagaacttt tttcggttct ttttcttttt tcttaaaaaa tccaaacatc attttcccta
    29521 ctttcaatta aaatgctttc aaaactgggc ttaagatcca ggcatataag agtgctgcgg
    29581 ctactgtatc tacatcggtt gctgtagcat tgttgaatcc catcgaatta aagatcgtta
    29641 ctagcaaagc gggcaataat gtaataaaga aaccatgagc aataccgcca atgatcgctc
    29701 ctcggcgtcc gccaactgcg ttgccaaata tccctgctgt ccctccggca aagaaattgg
    29761 ttagcatacc aggtaaaatc atcgctaagc cgaacatggg caaagtaaac atggcaataa
    29821 tcgtaccaat cgttgtcgta atgaaaccta aaatgacagc atttgggcta taaggaaaga
    29881 aaactggaca atctaaggca gggatcgcat caggaactaa tttcatagct atccctcgaa
    29941 atgccggcac gatttcacct aacagtagac gtacaccagc tagcaataca tacacaccca
    30001 ctacgaattg gatggcttgt aaaaaggcaa acatgatata gttttgatct cctgataatt
    30061 cagaagcgaa tggttcgcct gcaaataaaa cagtgatgat atatagcggg accatcacga
    30121 ccatgacaga caaataagta tcttgcagaa attcgaaatg tgtcggcaac ttgatatctt
    30181 cgatcgactt tttattttcg cctttctcac caaaaagctt agcaacaccg gcttcaaata
    30241 agtacccgat cgtacaaaaa tgacctaaag caatatcatt tgatccagtg attttacgaa
    30301 tgatcggttg cgctactgcc ggcatagcaa tggcgaaaca agctccgata aaaccaccga
    30361 ccaagatcaa tacgatccct cgcagaccgg caaaatagcc aaagactgtc gtcatcgttg
    30421 ccatccataa aattgcttga ccagttaaaa aaatgtactt ccattttgtg aaacgagcca
    30481 aaataatatt gaatacaaaa atggctaaga acgtcagtgc aatatctcta cctaaaccca
    30541 aatcattcat tgcttgacca ttgatagatt caatagaagg gatgatccct tgcatatgaa
    30601 atccttcagt aaagattttc ccgaaataag ttaaacttcc aacgatgatg ctcgaaccgg
    30661 cactcaacac ttgaaatcct aggagtgtct ttaacgtgcc agagatgact tgtcctgctg
    30721 atttcttctg cagtagtaga cccaacatag caatcaatgc aatggtgatc gatgcctgag
    30781 tcaaaatatt ttcgatgata aaattgataa cgcccatgtt
//
LOCUS       DACIRY010000025        30309 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.025, whole genome shotgun sequence.
ACCESSION   DACIRY010000025 DACIRY010000000
VERSION     DACIRY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.025"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            4..1086
                     /locus_tag="IYE22_000916"
     CDS             4..1086
                     /locus_tag="IYE22_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9506146.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1304..2446)
                     /locus_tag="IYE22_000917"
     CDS             complement(1304..2446)
                     /locus_tag="IYE22_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9506147.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2451..2636)
                     /locus_tag="IYE22_000918"
     CDS             complement(2451..2636)
                     /locus_tag="IYE22_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ9506148.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2990..3235)
                     /locus_tag="IYE22_000919"
     CDS             complement(2990..3235)
                     /locus_tag="IYE22_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9506149.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3229..3630)
                     /locus_tag="IYE22_000920"
     CDS             complement(3229..3630)
                     /locus_tag="IYE22_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ9506150.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="IYE22_000921"
     CDS             complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="IYE22_000921"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ9506151.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5648..7414)
                     /locus_tag="IYE22_000922"
     CDS             complement(5648..7414)
                     /locus_tag="IYE22_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ9506152.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7427..8221)
                     /locus_tag="IYE22_000923"
     CDS             complement(7427..8221)
                     /locus_tag="IYE22_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ9506153.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8231..9607)
                     /locus_tag="IYE22_000924"
     CDS             complement(8231..9607)
                     /locus_tag="IYE22_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9506154.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9727..10374)
                     /gene="eda"
                     /locus_tag="IYE22_000925"
     CDS             complement(9727..10374)
                     /gene="eda"
                     /locus_tag="IYE22_000925"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ9506155.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="IYE22_000926"
     CDS             complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="IYE22_000926"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ9506156.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="IYE22_000927"
     CDS             complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="IYE22_000927"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ9506157.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13041..14672)
                     /locus_tag="IYE22_000928"
     CDS             complement(13041..14672)
                     /locus_tag="IYE22_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ9506158.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14976..15647
                     /locus_tag="IYE22_000929"
     CDS             14976..15647
                     /locus_tag="IYE22_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9506159.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16372..16707)
                     /locus_tag="IYE22_000930"
                     /pseudo
     CDS             complement(16372..16707)
                     /locus_tag="IYE22_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16804..17256)
                     /locus_tag="IYE22_000931"
     CDS             complement(16804..17256)
                     /locus_tag="IYE22_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506160.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17263..17811)
                     /locus_tag="IYE22_000932"
     CDS             complement(17263..17811)
                     /locus_tag="IYE22_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9506161.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17856..18188)
                     /locus_tag="IYE22_000933"
     CDS             complement(17856..18188)
                     /locus_tag="IYE22_000933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506162.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18202..18432)
                     /locus_tag="IYE22_000934"
     CDS             complement(18202..18432)
                     /locus_tag="IYE22_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506163.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18654..19196)
                     /locus_tag="IYE22_000935"
     CDS             complement(18654..19196)
                     /locus_tag="IYE22_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506164.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(19217..20308)
                     /locus_tag="IYE22_000936"
     CDS             complement(19217..20308)
                     /locus_tag="IYE22_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ9506165.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20316..22526)
                     /locus_tag="IYE22_000937"
     CDS             complement(20316..22526)
                     /locus_tag="IYE22_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506166.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22542..25070)
                     /locus_tag="IYE22_000938"
     CDS             complement(22542..25070)
                     /locus_tag="IYE22_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ9506167.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25122..25532)
                     /locus_tag="IYE22_000939"
     CDS             complement(25122..25532)
                     /locus_tag="IYE22_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506168.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25545..25763)
                     /locus_tag="IYE22_000940"
     CDS             complement(25545..25763)
                     /locus_tag="IYE22_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506169.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25760..26752)
                     /locus_tag="IYE22_000941"
     CDS             complement(25760..26752)
                     /locus_tag="IYE22_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9506170.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26831..27742)
                     /locus_tag="IYE22_000942"
     CDS             complement(26831..27742)
                     /locus_tag="IYE22_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9506171.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27761..28105)
                     /locus_tag="IYE22_000943"
     CDS             complement(27761..28105)
                     /locus_tag="IYE22_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506172.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28125..28681)
                     /locus_tag="IYE22_000944"
                     /pseudo
     CDS             complement(28125..28681)
                     /locus_tag="IYE22_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28762..29262)
                     /locus_tag="IYE22_000945"
     CDS             complement(28762..29262)
                     /locus_tag="IYE22_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506173.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29263..29808)
                     /locus_tag="IYE22_000946"
     CDS             complement(29263..29808)
                     /locus_tag="IYE22_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506174.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29805..>30309)
                     /locus_tag="IYE22_000947"
     CDS             complement(29805..>30309)
                     /locus_tag="IYE22_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ9506175.1"
                     /translation="PLFFNFYEKRFEIANREKISELEALTKYGIYNRYELRLANEKAT
                     QAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARIVDKRWAKLFRSMEELDFTV
                     KPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQEGIDNAELNDEQQNYLEWMG
                     TSGGKIA"
BASE COUNT         9535 a         6144 c         4169 g        10461 t
ORIGIN      
        1 gatatcaatg atgatggctc aagaaaaaaa gtggagaaag ttggcggaga cacccgacca
       61 gaggccgaag ctgctttacg aaaagtttta tcagatattg acgaaacagg acaatacttt
      121 ttaggtacgg atactcgagt aaaacaatac cttgattttt ggatggagga atacgttaaa
      181 ctaaatctaa aatacaatac ctatgaaaac taccgattta ccatcaaaaa tcatataaac
      241 ggttatttag gaaagaaaaa acttacggat ctctcccctg ctcttttaca aaatttcatc
      301 aatgctgaat ttaaaaaggg ttactcgaag aaaacaatga ctattactca ctctgtcctt
      361 aagaatgcgc tgaatatggc ggtttatcct tggggattaa tcaagcaaaa tcctatgctg
      421 tatgtaaaaa taccaaaata tgaagcacga ccaacgacta aaaaagatct aaaaatcatt
      481 tctcttgagg actttgatca tatgctagaa attactcctg aaggacatcc tttctatatt
      541 cctttgaata ttggatttta tacgggaatg cgcgttggcg aagtttgtgg tctgacgtgg
      601 gatgatgtcg atttttcaaa tggaacaatt actgtagaga aacaaatggt aaagaatgac
      661 ggcgcatggg tatatggtac gccaaagaca agcagttcca atcgaacgat ttttattgga
      721 caaaccttgc tagcaattct gaaaaaacat aagaaacaac aattagaaaa tcgaatgaag
      781 tatggaaagc tctacattga ttcaaatgca gtatgtacga aggaagacgg tgagctagtt
      841 acgccaagtg tagtgaaatg gaatactagg aggatatcga atgcactctc cctctctttt
      901 aacttccatt ctctcagaca tactcatgct acacttcttc tcgaaaatgg cgcaaaaatg
      961 aaagaaatct ctgaacgatt gggccacagc agaatttcaa ttacgatgga tacttactcg
     1021 catgtaacag ataagatgag aaatgaaacg gtcgatatca tggaaaatct tagaaagaat
     1081 tcttgatttt tgccaccgaa aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa
     1141 catggtttat tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc
     1201 gtccattttc gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa
     1261 aaataggcta tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg
     1321 taatttcatt tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag
     1381 tagttttaat agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt
     1441 caattaaaaa ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg
     1501 caatttcagc tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat
     1561 caaaagaaaa aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt
     1621 ttaaatcttc aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg
     1681 tttcagcaaa ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc
     1741 tgccatttat taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac
     1801 ctgtaacaaa aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat
     1861 aaacactatt atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct
     1921 gagtttttat tttacctata gtatctacag gattttcttc aataatttca agtacaattg
     1981 cccttttaaa aactactctc aacttattga atatctgttt aatataattt ggtgataatg
     2041 gttgctcaat gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta
     2101 tatgaattgg tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta
     2161 aaatatgatc tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa
     2221 aatactctcg ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag
     2281 aaaaactttg ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc
     2341 ttctggtcac ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc
     2401 aagtgcctct ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc
     2461 caagaaatat ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta
     2521 cgataaaaaa agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta
     2581 gcatagtact gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata
     2641 gcaccttcct tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg
     2701 aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta
     2761 aatcatcgct ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac
     2821 agcaagaaat catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat
     2881 ctatttaatt ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac
     2941 tattagaaga ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt
     3001 taaattttaa gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta
     3061 cataggtaac gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat
     3121 aagaatgata atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata
     3181 agataatgga tagtggaact aataggtact gccgcttttt agatttatct accataggtc
     3241 ataccccatt ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct
     3301 catattgaga attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat
     3361 aatctttctt cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat
     3421 taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata
     3481 cctgccttca tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt
     3541 tcgaatagac ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg
     3601 tatagatatg tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac
     3661 taatacaaca atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata
     3721 tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg attaggctag
     3781 gctaatcgtt ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg
     3841 tattgatgac catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt
     3901 aaagattcct ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc
     3961 aaaattccac acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc
     4021 ttcgtaataa gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc
     4081 agctactgta tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat
     4141 ccatccatct agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac
     4201 ataccaacca taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac
     4261 atctggcgtt gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc
     4321 tttagttaat tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg
     4381 ttcatttgca atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc
     4441 cttgatcacc gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc
     4501 cacattatca tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc
     4561 atcaataact acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata
     4621 atgtgatgtt ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt
     4681 tacagcctca ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt
     4741 aaaataaaaa ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat
     4801 accaaattct tctgtatagg tatcgatcac tttattatca tcaattaaac taattttcat
     4861 actatataaa taagcgtcta gaggttccca aaaaattgga ttcttaagct gaatcattcc
     4921 tttctttcct tcagcttcag ctacaagttg agcctcttcg tcatatatcg ctattaagat
     4981 ttttccggtc cattctgtat cgatattgaa ttctacactt gcatccaaat cagttattct
     5041 tgtattaata gtaatatcac ttatatagtc tttcggagtt gtatataact taacaggtct
     5101 atgaatacct gcataattga agaaatcaaa attaactaag ttttcctttt ttatatcccc
     5161 attttcctgt actgtttcct tcataatccc cacaggtaaa gtactatcgt caataatatt
     5221 gtttacagca actgtaattc gattattccc gtcttttaca aaccgattaa tttcagactc
     5281 aaatggtgta aacccacctt tgtgttgagt aataaacttt ccattcaaaa aaacttttgc
     5341 ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatc ttttaggaag
     5401 agtcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa
     5461 taaatcatta taagaagagg gaactgaaat cggataactc tctttttcat ttattggttc
     5521 taaagcaagt tcttcctgta agtcatcatt tgtatctaat ttaaatctcc agagaccgct
     5581 taagtcaatt acttgcctcg tctctgtcat aatgggatac aacatataaa ccactcctat
     5641 ctaaatatta aataaatgta tcctttaaac cacaataagc atctattgga cttactccct
     5701 taaattcaga aagacccatt agtttgtcca ccaatatttc taaagtttct tgttttccat
     5761 cataagtatt aatataagtc tttacttgag gtacatcagc caaatgaaac ggacactgta
     5821 cagatacaaa aattgttgga atttcatgaa tgtaaaacgg tatatccgga gttcctttac
     5881 ttgctggcca gacaattcgt tgaactgtgc ctggattaac attagctata ttaatgatca
     5941 agtcatattt tttagttaaa tttttaattg gtctcttagc agcatatatt ccagctatat
     6001 ttttaatttt ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa atgtattttt
     6061 caacttcaaa tccttttgct gacaaaatct gtttcaataa atcaatggct tgaggtctat
     6121 ttccagcaac tacttcacca aacccacctt ttaccccatg tacatctact agtaaaatcc
     6181 ttttatattt ttctggtaca ataggccaaa tatcttgttt atctttaact aatgtaattg
     6241 cttggtcagc cacctcttta aaaattgatt tattttcaat taaaccaatt ttttccagag
     6301 cttcttgctt aggtaataaa atttgttctt ttgctttttt gtgtaatccc aatttagctt
     6361 ttaaccctaa aattcttttt aaggcatcta ataaccgatc atcagtgata attcctttac
     6421 gatagccttc catcatataa ttaaagtctt catctggatc attaaaaaat agaaatagat
     6481 cacatccagc tgcaatagaa gtgggcaata attctgatcg tttcattgcc ccagtcattc
     6541 ctaacatatg actagcatca gtaactacta atccattaaa tccgagtttt cctctaagta
     6601 agtcagtgat taattctttt gataaagttg caggaagtaa atcttcatct ttcagatcag
     6661 gattaaaatg ttttgaataa gctggcatat ggatatgacc tgccataatt gaaggtaatc
     6721 cagcttctat taagcctgag tatacttttc caaaagaatt atcccattct tccgtaccta
     6781 aactattaat actaaaagat agatgctgat ctcgttcgtc aataccgtca ccagggaaat
     6841 gtttagctgc aggcaaaatt ccactctcct gaataccttt catataagct aaactaagtt
     6901 ctaaaacttt atcagggttt tgtccaaaag atcgactaga aatgatagga tttctccaat
     6961 ttctattaat atctacaata ggagcaaaac tccagttaca tccaatagcc gatgcttcaa
     7021 tcccagaaac tctccctaat tcataagcat atttcgcatc atttgttgca ccaattttta
     7081 cttctacacc aacttcagta ccatctgtac aagctccgtt accacctgct tccgtattag
     7141 cagcaattaa tagaggaatt ttactttctg tttgtaagat atagttttga tcatatacct
     7201 catcagcagt tcctggatta tatcgtacag ctccaatatg gtactgattg accatattac
     7261 tcaagtaatt tttatcacga ctagatccca tattaacaaa caattgacct attttttctt
     7321 ccaaagtcat agaatcaatc gtctcttcaa tccaagaaat agcatcttca tctaaattat
     7381 atggtttttt tcgtaaatct acttttatag tcattttctt tctcctctac ttattagtaa
     7441 gatttttcca aaaagtatct atatattttt gttcaatatc tacataatct cccgataaac
     7501 atttaaataa aacatcagtt cttaagtttt cccatgattc tttttcttta ttgaccaaaa
     7561 taggataaaa gaaaacttta tttttatttg cagcttctag atcaccagga gaatcaccaa
     7621 tcattaatat cttatccaaa tcacaaccat tttcaattac acttcgaata atatcttctt
     7681 tttttccttt atcttgacaa cacagttcat caactaatga taataattca tgtctttccc
     7741 attcttcttg aacagcttcc ttattagctg agcttacaat aattatcttc ccatattttt
     7801 tcagagcttt tatagtatct ctagcactta aaaagggttt atctaatcct tttgcttgac
     7861 taatctgttg attgacttct tccgaccatt ttaaagcttt taataaatcc tctgactgag
     7921 aatattctat ttccctcttc aaagatggat tagacaattc ttttgttaga gatacccagt
     7981 gctttaatcg attaatcgat gttacatcat ttttttctaa tgttttaact aaacctaaaa
     8041 atctgtttat tccacgagtc tctgaatata aattaatatt attccaatcc tgtaggaatt
     8101 tttctctatg atgaacatca aaaatctctg cagcaattgg tccgaaaaag agattgtgtt
     8161 tataagtcat cgtatccata acacagccat ctgaatcaat acaaaatatc caattattca
     8221 tatttctctc ctatttatgt ttttgtttta aaagctcgca attttctaag actttatctt
     8281 tagataatgg ataaaggaaa aataaaatta atcctacaat cagaaaacaa attgatggaa
     8341 ttaatgttga agcgttatat aacttttcta aaacatctcc cgtttgtaca tttgctaaag
     8401 agtcatagcc tataatagaa agagtccaac ctccgacgcc tccagctaga gcttgaccta
     8461 tttttctagc aaaagaatat accgcatata cggtcccatc ttctctttga tcagacttta
     8521 cttcttgatc atcaataaca tcagtaatat ttgcccaaat aacagtatta aacacaccca
     8581 aacccatata gccaaccgaa ctaagaatta agaatacata catattacta gcttttatca
     8641 aaaataagat caaatatact attgctgaaa agaacattcc tactgatgcc agttcttttt
     8701 ttccatattt tttacttaaa aacaaagtta aaggcgaaac aactattaaa accaaaatag
     8761 gattgataaa attcattaaa actattccct gtgcatcatt atagtaatca ggaaatacat
     8821 aattattcat actcataatt aataattgag caaggagtaa gaaaatagcc gcaccaatta
     8881 tagaaagtaa tgccctattt ttaactaacc cttttaaaga ttttaacaat gaaactttgt
     8941 attgagattg gtcaattctt actcgttcag tagttagttt ataacatatt aaataacaaa
     9001 taagtgcaca tatcgaaaat attcccgcaa caattgtaaa agttgatcca ccacgaacta
     9061 tttgagcgcc tgaatcatct cttgtataaa taaataatgg tgttaataca ccaatgacca
     9121 aacttgctaa agtagctcca acatttcgga atgtcgatag cattgttctt tcttctgtaa
     9181 tagaagtaat agctgatgcc attgatccat aaggaatatt tatagatgtg taaaaaatac
     9241 tcccccataa tatatacgta acatacatgt atactacctt aacgctcata gaagctgaaa
     9301 tcatagaagt ttgatacatt aaaaaacttg ctaaagcgac tggaccactc attcttaaaa
     9361 tccacggttt aaacttaccc tgcttagttg ctttcgatct atcaacaata cgccccatag
     9421 ttacatcagt aactgcatcg ataaatcttg ctactaaaaa taatgttcca acggcagctc
     9481 cgcttatccc caatactttt gtataaaaga tcattaaaaa agaactagca aaaatgaaag
     9541 taaaatcgtt tccaaaatct ccaaacatat atcctatttt gtctcttata ccaaattttc
     9601 tttccataca attctctccc ttctgttgtt aagcgttttc attctgatga aagtacgtac
     9661 tatactctga agcgtaaaga gtcagtattt attttttaca tttcagagta ttatttgttt
     9721 tttctcctaa taattcttta tctcataaaa cttatctata tatttactag ctactcgact
     9781 tacttcttca aaatcacctt tttcaacaga agccaataaa ttcccaccta caccaactgc
     9841 aacaactcct acttcaaacc aatcttctaa gttctctata cttactccgc cagtaggcat
     9901 tatatttacg tgtggtaatg gagcttgata ggcttttaca atggatggac caaaagcaga
     9961 acctgggaat agtttaatga tatctacgcc agcagtaagt gcctccttta tctcagtaat
    10021 tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctcag ctgtttgtcg
    10081 atcaaaagat ggactaacga tatattctgc cccagccata attgcgatac gagcagtaaa
    10141 cgcatctaaa accgtaccgg ctccgattat tacatctttt tgatcagcat attttttagc
    10201 aatttctttg attatttctt cagcattagg aactgtaaaa gttagttcaa tccctttaat
    10261 tccacccgca ataattgaat ttattgcttt aatggcctca tccttatttt tcccacgtac
    10321 aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt tcatatctat
    10381 ctccttcctt tatggataca tactttgtct agctgtacta acttacaaag aataaattta
    10441 agcttttatt tcaagtatta ttttctactt ccgatttcaa aaaaattaaa ataattatac
    10501 gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc atctggaatt
    10561 cggcctgctt taacccactc gcttagtaat tgacataata ctcgacgaaa atactcatgg
    10621 cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa
    10681 ctttgttgtg ccatcgtagt caactgttga cgcattcctt cagctgtatc attgaaccac
    10741 caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg cattgctgtt
    10801 gctagtggaa gccaatcagt tggatttaat gaatataaaa ttgtttttgg taagcaccca
    10861 ctcttttcag cggtatcgaa caatgctaca acattttgtg tcaaactagc ttgatccccc
    10921 atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga attatttctt
    10981 aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga catcaaatgt
    11041 aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct
    11101 aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata aaaataagta
    11161 ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aatgatccac tcgttggctc
    11221 aatgcttgct tcaacccatt aaagtctttg attggtatag ccgctgtttg actaagtttt
    11281 aaaatatagt tcgaaaagcc tgatgatttg atactccaga ccttatctgg acggaaagta
    11341 ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatacca caagtcatct
    11401 gtaggatcat ccgttgtaca gatggctttc acatccatca tctccaacaa ttctttcggg
    11461 cgataggaat ccgtttgaat tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc
    11521 tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa
    11581 ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt ttcataatca
    11641 tcacctggac cactgatcaa ctcttcttct ataccattag ctcgcatcaa tcgccattta
    11701 taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt gtattgctta
    11761 ttttctagca aatatgctgg gtctaaatga caatgatagt cgataattgg ttggtgtttt
    11821 gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt cttatctaaa
    11881 aacatattta aacactcctt tccttccgtt agtttttaga agagaaaatt tttcctctaa
    11941 ataaactccc ttatataatt atttactcgc ccccttttca agcgcttccc aaataccatt
    12001 taaataaact gcacctagag ctcggtcata taatccataa ccaggtctac cggtttctcc
    12061 ccaaatcatc cgaccatgat ctggacgaat atagccatta aatccattat cgtacattgt
    12121 ttttaatatc ttatacatat ctagagaacc atattctgat ttatgcggcg cttcgtaaaa
    12181 atcgagtcca ttgtcactta aatatttgat attacgaaca tgtgcaaaag gaatccgatc
    12241 ccgttgacaa aattcagata gaatttcata gacatcatta tctggtcgtt ctgcaatact
    12301 tccagtacat aatgtaatac cattagaagg agaatccact acattacaca accaatctaa
    12361 atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg
    12421 gtgaactgcc attttaatcc caacttcttc acatgtcggg ataattgctt tgagaaaata
    12481 tgcaaagttc tctcgtaatt tctcttcatc aacttcttta taggcttcta ataattgctt
    12541 aatttgtgat aaccgatgag gttcccaacc cggcaattca aaattattag tagaattttc
    12601 tacttctcta ataatttcct ccggatcagt agggatattt tttgaaatat aggctaacgt
    12661 agaagaccca tcatctaaac gataatccaa atccgatttt acccaatcaa atactggcat
    12721 aaaattataa cagataacct caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata
    12781 attctcaatg tattgatctc gtgttggtaa accaattttg atatcatcat gaacattaac
    12841 actctcaata actttcaagg taagtccatt ggcttcgact tcatctttta aagatttaat
    12901 tcgttctctc ggccaaacct ctccggctgg tatatccatt aatgttccaa caattccttt
    12961 cattgctggg atctgacgaa tttcttgtag tgagattttt tcttcatttg ccccatacca
    13021 tctaaaagtc atttccatag ttactttcct ccaacttctg ttatatattt attgattgtt
    13081 ttacgaactg cttgaggacc tactagcatt tcgcaaaaga ttttttcagt tttctttcct
    13141 accccaactt gatataaatc taaaccaaag atttctttat tttctaaaat ttcctgtacc
    13201 gcacaatgaa tctttgaagg atcttgttca cctaatttca atactgataa tttatcctgc
    13261 aactctacca ataacggatc aggacttggc acaaaatttt ccccttgatc atcgactgcc
    13321 agtaaataac gtaaccaagc agcaatagcg ataggaataa atgttagatt ttctgcattt
    13381 ccgtcttttt cgacatactt tttaattgtt tcaccatagc gaatcgctac tttttgagaa
    13441 gtatctgttg caattcgttg tggtgtatct ggaatatagg gattaggcaa acgtttatta
    13501 attacttcat caataaattg ttttggatca ataatgcctg gattttcaac tactggtaaa
    13561 ccttctaaat aacctattcc tttgattaaa ccaaccaaat catcatcctg catctctgct
    13621 gaaattgatt gatatcctag cagacaacct gttactgcca aagcagtatg aagtggattc
    13681 aagcttgtcg taaccttcat gatgtctgct ttatcaaccg tctctctatc tgtcataatc
    13741 acaccagcct tttccaattt aggccggcca tttggaaaac tatcttcaat aactaaataa
    13801 tgaactacct cagtattagt gaaagcagca atattggtat gtttttttgt gtgaatgata
    13861 tctaaatcct cgagccccat catcaataga tcttccttta cattctctga aggatttggc
    13921 gtaattcgat caatcatact ccaaggaaaa gtcacttttt ctttatcctt taaataagca
    13981 ataaattttt cagaaacata tccttctttt tgccaagctt ctgcaatcgt aaaaatacta
    14041 tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac
    14101 tctccagcat taaatctgct atataacaag gatgttacaa tactcatcgt atgttgagga
    14161 ttgctaggtc cagatttgat atcagctttg actaccgaaa agaattgccc tttagaatct
    14221 tttaaactgt aacctttttc agtaatagta aacgttgtaa actgtaattt aggatcttca
    14281 aaatatttaa ccactctttg atagtccgct atattcttcg gattgcaata taaactatca
    14341 gccgttactg ccaaaagtct ttttttgagt tcaccatctc cactcataac aatttccaaa
    14401 atgtcattgt tatagtcatg ataagctttt tccactactt cttcatcaaa tgtttcacac
    14461 acaactactc ctgtttctaa ttcattttga tctgctaagc attgagcaat ttctgcatga
    14521 aatcctcgat aaagatttcc tccaccaaaa tgaagccata ctggatgttg ctggccttct
    14581 tgcttaactt ctttgacatc aaaacacggc agtagaatct gcatttcatt gaattcttcg
    14641 atagaatttt gccaattttt aatcgaaacc ataattaacc tcctcaaact gatatgttag
    14701 ttttgataca ttttatattt taaaacccat tgtaaccgtt gtcaatataa aaagggaata
    14761 aacttgcttt tttctaccat atcagaatga ttgtattttt tattcagaaa aataaaatac
    14821 tccttctttt tggactgatt ttatcgaaac ttattttttc tttttttcat gtattctttt
    14881 atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa cagaattaaa
    14941 atatcagtta aaataaaagt taaggagcat tgtgtatgac aattaaaact agtctccaaa
    15001 atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa cctggaaaaa
    15061 aagtatcaga aaaaaaatta gaagaaacac tttctattgg tcgtacacca atacgtgaag
    15121 cactgattca actccgacaa caaggtcttg ttgatacaat tcctcaaagc ggaaattata
    15181 tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa catttggaac
    15241 gacaaattat gttagagtgt tgtgccaaaa tgaataacca aaaaaaacgg attttagaaa
    15301 ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt ttttttcata
    15361 atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa gtatggcaat
    15421 ggttagaaaa taacaataca cacttagaac gatttcgatg gctacgagta aaaacggaag
    15481 gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca ttaacaaaac
    15541 aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg cttaatgagc
    15601 aaaatattgt tttagctact catccaaaat atttcaaaaa tatataactt ttaactcttt
    15661 ttaaattaaa tggttgttct gatcagaaaa atatcgcttg ttttcttatt atgtaatata
    15721 tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg ataactaatt
    15781 atgactgctg ataacttttg atattaaact agtatctcca atacaaataa aaaatagata
    15841 tcttgttcat caaaattagt tcgtggatac ttaccttcat ttacatcatt cagcggtttc
    15901 ccattttctt ttattctatt atttcatttt ctaatagact tggcttcatt tcgcaatact
    15961 ttttttcaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt ttgtgacatg
    16021 ataagtatgc tcctttttct cttttccact aatacaacga tcaaagggtg cttttttcaa
    16081 aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg tttcaactac
    16141 atcttatatt tcacaataga ataaatagcc ttttgcatag ctgcttaact tataagaaat
    16201 tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat tcttcttaca
    16261 gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta ggattgatgc
    16321 cgattactta tattctacgt atagttttag tattcttatt ttttatcacc agtaaccaat
    16381 tgagaacagc cctaaagcag aatagtcttt tatctcaaaa cgcttaattt caggcttagg
    16441 aatatgaata atcttttgag tatattcttt ctcttttaat cttactgaag aaataccact
    16501 gcctatcttt tttacaaata tttcatttgg tgtacagact aataaaacaa tatttgtaat
    16561 caatccttgc actaaagaat taaaaggaag actatttaaa acagggtgtg cataactata
    16621 acatgtgaag tagttatttt gaactttaac ttttaaaaac tttaaaaata atcttatttg
    16681 ttcttctaaa caatcatcta tactcatata atcactccac taattaatct ccccaaaaag
    16741 acacagcatt aagagatata ccaacacaac agtgcaaata actctacatc ttccttcgca
    16801 accttatttc aataaaccat aaaaattatt ttttaataga aactgaaaat taaaactaga
    16861 aaaagtagtt ttaccgaatg taagagcatc atcaccatca atatagaaat acattctttt
    16921 ccagttacat ttaattttta aacaaaattc tccccaaaaa ggaacttttt ctagtgaaaa
    16981 cttggtaatc tctttgtaat taattcttgt taaagaataa ttaaacactt catttttact
    17041 tgttattttt tcattgacta taactagttc atctggtaaa aaaattaacc aacaaacatc
    17101 gacaaacagt aaatgatata atccatttcc acctccaagc atattcaata ctggtgaaca
    17161 tgccttagaa acacgaatat agttatggtt taaatagata ttttctttaa acattgcttg
    17221 tttaaaagcc atatgctctt gttctaaact tttcataaac ttttactcta atgcacagct
    17281 agcagcagct gctgcaattc ctgctacaat atttacctta gctaactctt tagctgcagc
    17341 ttttcctaat tgtttttctg caatttttaa taataaatcc gcagcttttt caaaattaaa
    17401 agcttttaat gcttcaataa aatctccctg cgtacctata gcccaagcca ctcctcctcc
    17461 aggaagagaa ttaatggcta cacatccagc atatcgtttc caatcaactt tctccattct
    17521 aggaacaata atatgttcat aaaagactct tgctgctctt ccttgttcgt tatcttgact
    17581 gattcgttca cgaatagcac caaaatcctt tatatggtat acaccatgtt cgtctaaaaa
    17641 accaactgta tcaaaataaa aagttaattc atctactaat ttattcttta tttctaaatc
    17701 aactgtattc actttttctg tggcatacac acttgttcca gtactaacaa aagtacttaa
    17761 acctaataag aaaacaccta aaacaccaaa tattttaatt attcttttca taatttctcc
    17821 tccataaaaa ataagttact ttttaaatat cttgtttatg aattcattcc ccaaaggcct
    17881 tttaagattg atacagcagt ccgttcactt cttttagact tgtcatacaa gtcttttaaa
    17941 tgatcccata tcagactagt ttgtctatct agcttcaatc cattaaacgc taatgaacgg
    18001 ttcatttttt ctattacttt agataaagct acagaaattt ttataccttc acaacgcgca
    18061 tcttggattt caataatagt atcatcaatt agtttttgaa tttcttgcct atcaacatat
    18121 tcaatcttag aaagagaatc ttttaaagct actaatgatt caacgatttc ttttttctct
    18181 tcagacattt tcttcctcct tctagtgacc cgtaaagcca tgtagagcag agtccattga
    18241 accagtaatt acactatcta caacgccatt ccaatttact ctacattttc catttttcag
    18301 atttgtcggt ccatacggat agcctttttg acattcaatg ttcggattat accagatagg
    18361 attataccat tcagccgcag aagcagattc agtcgataca aacaatccca caccaacaaa
    18421 tccaatacac atcacactat agccaattag ttttttcatc tcgtatccat ctcctaaaaa
    18481 atagaatagt tcttatagtt attcataaga catatttttt ccgtttttgt tttatccttt
    18541 ctgtcctata acaacttttt tcaagtaaag aatgacatag tttttcttaa aaaaacagtc
    18601 tgtttttttc tatttcttcg gtgtataata aatatgagtg ttcatttttt attttaatac
    18661 gcactccgta cataataaat cttgatcgca tcaatctttt gagcttcttg atctactttc
    18721 aattgaagta atacaaattg agtaacctta ttcattgttt cttctccaaa taacaaatat
    18781 tgatccggat tctctatatc ttgtgtcacc tggtaaagtt tcccagttgt tttcatttgt
    18841 acatgcgggt ctacatcaat actattgtct ttaatacaac gatctgtcat atagtccctc
    18901 acagcttgat tgcgtttata aacatctgtg aaattactcc attttctagc aaaatctttt
    18961 aagaaatttt ttgtcatttc atctatttgc atttgttgcg ctgtctcgtc ttctaatgaa
    19021 ccaatttgac tgttactttc agtactttga agagttccac ttgtttcttt tactgcttcg
    19081 attgtttctt ctgtcgttct caattgtgtt ttttgttttt cagaagaaaa ccaatgacaa
    19141 ccagtaagac ctatcaaaca taaccaacta aatactaaaa ttatcccttt tttcacgttt
    19201 ctagtcactg cctttcttat ttcaccttac gaaccagact acgaattttt gtttgattgt
    19261 acgtgcgttg ataccgagcc ataatccgtc cttgctcggc attctgttcc acactagaga
    19321 aatgttgtcc gccatatgaa acatctgtta taattccggt gtgaccatag attccaggag
    19381 acaatgcacc acctgcttgc caattcacca catctcctgc ttgtaaatca cttggttttg
    19441 ggttcatgat tacttgccaa ccataggttg tccagttata atcttcccct attaacatcg
    19501 caaacatgtg tccagaaccc attaactgtg ggccacctaa ttgagataca taccatgccg
    19561 taccaccata acattcccca ttatttacgg atcgtccaat gatccgatcc aaaaccttcg
    19621 cattgccttt actttttcct gcaataagcg aagttttttt tgttggtagg ctattccaat
    19681 gcttgatcgc aaaagtatca tattccgtta agttatagga cgttatgata ctcattgctc
    19741 ctcgataata cggactccct tcggtaatac ctgctgccgc atagcctcca cgacctagaa
    19801 tcaataaaca acttccagga tctttattgc ccacacattg attaaagttg ctttggctcg
    19861 ccaccataaa gccataatag tcataacaag cttttaagtt cggaaacttc atatagaaac
    19921 caccttcaga accaccaatt ccacgtgcag aaccttgtgg gtagggacaa ccaggaaacc
    19981 aggtaatccc aaaaaagttc gtatcgtttc ttccagattg tgactgtcca taagctgatt
    20041 catagcctaa ttgagaaaga atcacactcg gtaataactg atactttaca gctgcaccaa
    20101 ctgctgcttg cgcatgttca tctgtgattg gaccacgtcc tttaatctct gaaaaaatcg
    20161 gtaatccttc ggtatattcc ccattaaaag cgacagctag acctgtagct tctcctccat
    20221 gctcagaaag ttctcctgaa aaaaagaatg gcaatagaaa aaagggaaga ataaagaaaa
    20281 gaagacaacc tagacaccct ttcttcattt cttcctcatc ctttcttcga taatggtttt
    20341 tggactgttc gtccttttgg taaagtaaaa tcagttcctg gttttgcgtc caaaatcggc
    20401 agtgttttag gatccttttc tattttttcc gattgctctg ttggcttatc taaagaagaa
    20461 aatgcaggtt gtaacgattt tggtgcggaa atcgacggtt tactttcaga cgaacgttgc
    20521 cccggttgtt gcacagaacg atttcctgat agagtggcta ccgacgaatt aggaacagag
    20581 tattggttca ataactgctg cattgctgga taactcccat ttttcgcctg atttttcact
    20641 tggcgaatct gttgtgaaag agaaccagca gctgttaatt gtggcacggg tacttttcca
    20701 gaaggcaacg gaatcgattg accaacaaaa gcgatacctt tatttaacaa tggttttctt
    20761 tgattattcc cttgttgctt actcaattgt gccaatgatt gtacctgttg ttttggtctt
    20821 actgaattca tggacgaatt tggaatcaaa ccatttgaaa gaccagaatt ccactctgtt
    20881 ttcgatttgg tagaagacat ttcaggctct accccatgat aacgaacatg acgaccacta
    20941 taaggcaagg cttgatttga acgcccacct agttcaaagg gattaaccac ttgcctccct
    21001 tttaccgatt cttgtggtgt acgtttttgt tgaaactttt gaaatccatg tatcgctgca
    21061 gaagcgagtg gattcgttgc tcgatacaca ttccctgcta atcctgcacc tttgattgcc
    21121 aaattttgtc cagcagttga tagttgacca ggcagttgtt tcatttgaga caaagaatga
    21181 cttgctatgt ttttcgtgtt gtttatagtt tgatctgcag ctcctaaccc tagtttcttt
    21241 aataaatctt tccgttttac aatgagtaaa attgatagga gtgctttgac aaacatattt
    21301 cctaaaaaac caaaaacagt attcattgga attaaatcgt ctactatgtt atagatcaaa
    21361 aatgccacca aaataatcac actgtaaaaa gatttttgaa acatcaagcc caaaaaggac
    21421 tttaaggtat tcatgagcat ataatcaaat actggaacaa aagaagcgag taatgaaaaa
    21481 ggaatcacaa gtaataaaca caacaccaac atttgaaaga taaaacgaat catgccaatc
    21541 actacaagtg gtaatccaat acttagatta agcaatggca ccatagaacc aacgactgct
    21601 ttataaacag ctttagttgg ttgaatatag acacgataag ccttcttttt ccctctatct
    21661 tctcctgcag cagtggtcac ttttgtctca atatcagata attcttcact tgacgcttcc
    21721 ttggctagaa attcttgtgg ttcaatctct gcttgataca attcttctaa atttgttgtc
    21781 ccataattta acaatatata cggatcaaca acagccgctt tataataaag ttggcgcact
    21841 ttttgaatgg cttccttttg tgtcgttggc actaaaacac cattgacatc ttttgtcgcc
    21901 aattcatcta gactatttgt agcagaaaaa ataaagcctt ctacctccac ggacaaatca
    21961 tttaaccctt tggtaaaagc ttctccttta ctaggtacgt tactaccata gccaaaccaa
    22021 accattgaaa aaatgacaac gaagagaaat cgaataaaca ccatttttgc gtaatgtttc
    22081 cctctaaata tacgaagagt aaatagatat aacacataca agacgacaat ccccgcacca
    22141 ataggatcgt atatttttga ataaatagta tccgaaaaag tgaaaaactg gtgaattaac
    22201 tcgttcattg cagctccttc atagagcttc tcaatgacaa aatcaaggcc ttgccaaatc
    22261 aatttactta aactaaagaa catattcgtc atcattttta gaaaatctgt gccatttgcc
    22321 ccaaataaca ttttgcctaa tgaaaattca ctatcagata aataagaaga aaaggtatca
    22381 atatgaaatt tcccaatctc atagatcgat tgatccacct gcttttctgc cgcatacacc
    22441 aatccaccaa caaaaagcag tccgaatcca atcgttaaaa aaaacaaaac gcaccatttc
    22501 ttttccttat gctttatata agccattgtt tcacctcatt tctattgcat gattaacgta
    22561 tcctgttctt ctacttgaga gtgacttgtt ttttccgttg tttgtaatgc agcggtcatt
    22621 tcttcaaaca aactgtgtac attgagtttt cctacacgtc catagggatc taacatccaa
    22681 cactgtcctt taatcatgtg gctcatttca gctaagttcg cttcatttac ttccaaacgt
    22741 aaatgattca aaatcttttc tcgctcactg tcttcatcaa aagcaaagac gacaccaaaa
    22801 ttcccttttt ctttatctga acctaagtca ccaataaatt gtgtatctaa cactaattga
    22861 ttcatctggg aacgccccac acgtgccatt gctgcaataa tactacgtcc tgcgttcgaa
    22921 ttattaaata cccatgcttc tgtaaaataa atcgctgttt tcttactact atctcgtttt
    22981 ccaaacattt cacaaaaacg acctaaacta atcatcaaac acaaagactt tcgatccgca
    23041 tctgtatata gttctggtcg gacattgtca tttggtaatt tcaaatcttg gatctctaaa
    23101 atcgttgttt tggcattaaa gtctaatccc gggttttctc cataagaaaa tcctaaacgt
    23161 aaaatcgaat cttctaccgt caagcgtaaa aactcaccat attctcgaac ctctttttgt
    23221 tccatttgtt ctaaatagtc gaccacatgc atcaagccaa cttgttgtcc ggcttctttt
    23281 tgaactaaaa cttcttggat tccctttaat accgcccctt ttaaaaggtg attggtacct
    23341 aacggactta actcatcaat catatcctgg gctacttgtt tcgcatctac tcgggataaa
    23401 caaacaattg gatctaatac accataatta cggggatttg tagcatctaa cgtgacataa
    23461 ctcatttttt gcaaatgttc tacaaacaaa gggtaatgtt tctgataata cgggtcttga
    23521 atgagtttcg taaaccattt gcgcttttct cctttgggat ccacaaataa actttgtaca
    23581 ttcataaacg aactatacaa aaagagcaag ccaactaaaa aggtcttccc tttaccggtt
    23641 tttccagtta ctgctacatg aggcgcatca aacaacgcac ctttgattcc ttccgcaatg
    23701 gctagaagat tcaaaaagac aaaacgattt gacgcataaa ttaattcttc taatggtgtt
    23761 ttttctcctc gagaaccacc atcccctaat gtatcttgcc gaccaatata ccagccggat
    23821 tccataccta attctgatac tgctgaaaac aaccattcgc caactgcttc tacagttgtt
    23881 cggtgcttcc aatgtaactg ccgtcccaat ggttgcccta ataaacattt atagaacaat
    23941 tctcgttgat cattttttgc acggtggata tggataatcg tatgaagttg ttgaataacc
    24001 gttgcactat tttgacgaac ttcttcaacc gtttgaccgt atacaataaa acatgcgatc
    24061 caatcgacga caggttgccc attatctact cgttgcttta aatgttttac taatcctcga
    24121 acagttaata agcgttcact aggttcttca tcattttgaa ccaattcctt atgctctgtt
    24181 ttgaaccgtt ccgatagcca attcagtttt aaaccaatac ctccctgtcc tttcaagcgt
    24241 ggatagtgaa ttttcattcg caattctgct gggaaagaaa gatcctgagc aaattgaaat
    24301 agatcacaat ttctcatatt ttcaggtgtt tccccaatag gcaacaatgc taaataacta
    24361 ctccctaact catcttctaa ttgaagaagg cctttatgtt ccttcggtat acgtacatac
    24421 gttccttgat tgtctttcag tacttcccaa ttattttgtt cttctacttt gtgagccatt
    24481 cctcgaatat attgcagacg attgagatac aacagctctt tttttactaa gggacgaata
    24541 gaaaagctat aaagtaattg tgtcacttct tttacataag gatcactttc agaaaacaaa
    24601 gtttccaaat catctacttc taatccaaat aagcgtgcta aacttcctgc taaatctaac
    24661 cagattagat ttattacatc atgaaaactc tccaccgttg ctttttcttt gactttgatt
    24721 cctaaaataa atgaaggacg atagacttga ccttctcgtt ccaacaatgt cactgtttct
    24781 tttgcataat acgcaccaat tgcttgacta tcgggagaaa atccctttcc taatgtgtca
    24841 aacttcgtcc gtaaattcat tgttcgtgga tacatcacca actccacatc ttcataatct
    24901 gctaaaagca acagtaaacg agccatcatt tcttttattt cctgtgtctt ttttggtgtg
    24961 ttttttgaaa ttgttcgtgg aaaaacatga tagtaggccc agatacaatc ctctccattt
    25021 aacaaacaat ttttcttgaa tgcttttact ggttctttta caagcaccaa tgaatttcac
    25081 ctcccaataa aaaaggaaca accagttgat tgtccctttt tctaatcctc taaataaaca
    25141 tacttattca caaaaagtaa ttcttccaat gcattgtttg ataccttgtc ccagttataa
    25201 tacacacaat caccattcat catacgaatc atataacaaa accaatccca taaataaaaa
    25261 tgcacttttt ttccatcagg ttttagtttt gccagtgcta acacacataa ataggggagt
    25321 cctatataaa aaacaactcc tgctggtgga aaaaaagaaa tgacccaacc aataaatgaa
    25381 cgtaaaaata aatattcaaa gattacgacc aacaaaaaag ttgctgcatt gactaatgca
    25441 actggaaatg gaagacgcca attgcttgta atctgttgta cgcgataagg ttccgcataa
    25501 acaatcgaat aatcataagg ttcccaatcc acttcactat cctcctaatc cataaataat
    25561 ttttcaatga ttttaccaaa cgcatcaaat acacgtgttg gatcatttaa gaagaaataa
    25621 actgcggcac ctgctgcgat agatattaat gcatttccta cttttgattt cataaaattt
    25681 cgcaatccca gataaacaac caccattatt aaaaaatagc caccttgcgt taatataaaa
    25741 tctacaattc ccttcagtgt catttccgtt cttctccttt cctatccaac caattatgtg
    25801 ttaatttttc aacataatac tgatttgttt ttttcgtaat gacaatcgtc atttgttctt
    25861 tttgtaaaag tgacgtatca ccatctcgaa agacgaccgt tgcttttaca ataaaaggtt
    25921 ttttcgtttc tttataacgc aatactttca tctctacttg atcaacttta gctgtttgat
    25981 tgagtccttc aggttctttc atcatgtatg acatatcttt tgcattgttt ttcgcatact
    26041 tatctaagaa atcttccaca aatccttcaa catctttcgc ttccgacgca tcaaccgttt
    26101 cgtataatgt gtcatcataa gtaatcattc gttgtttttc tgtattcttt ggtagtgccg
    26161 taaaataagg ataagcaata atacgacagc ccttgtttga aatagaaaag ggaagattga
    26221 gtacttgtgt gattgtcaca cttgtttctt tcttactctc tggattcatc gagcgaatat
    26281 cataagttac tttataacta gcagttggaa tggtttcaat ttcttttaat ccaataaacg
    26341 aagcagcagt taattttcga acacctgaaa cacttcctgg ttcttgaata aagttttgtg
    26401 cataaaactc tttttttaat gtttcttgac gttgttgttc ttgttcatga ttatcgaatg
    26461 tattcatata caaagcaaca aaacgattca taaaggattc aatttcaggt gtgtacgtta
    26521 attctgaccg actttgtact tctttctttt gtaattgttg gatttcatct ttcaaaaacg
    26581 acacttgaac cttcgttttc atggattgaa aaaatgccaa gccccctgaa aaacaaagaa
    26641 ataaaaccaa acaccaaatc catttcactg ctttttttga atggattttc ttttgcttcg
    26701 gtactttctc tgtataaact ttttctttcc gccgtagttg tcgttttttc ataagctact
    26761 ccttctagta tgtttttact agtacgtacg attgacaacg ataagcttgt ttttctgaaa
    26821 aatagtccat tcaattaccc tcattttcta ttccttttct ctttctttta tatccaacaa
    26881 atccagcaat acttgtcaac actcctccta gaacagaaag tgcaacatgt atttcttcac
    26941 cagtctttgg taagactttt gtttttccat ctgctgtttt gatttcaata ttgccacttg
    27001 gaagtttctt tacaggaatc gacaaatcct ttaccaaatt cccttcacta tcttggatta
    27061 atggaatacc atcttttaca ccaacaattg tttgatcacc tgctactttt aatggggtgg
    27121 ttggtgttgg aatatgggtc aattcctcta cattactagt gacaatcggt acactcattc
    27181 cttgagaagt attcgtcgta atttcaccgc ttggggttac cgatactgtg acttcttttg
    27241 ctggaacaat aggtttattt tcttttggaa ttgcttctga tttttcaaca ggttgtttcg
    27301 gttcttcttt ttgttcttca ctggtagacg acatcgttgg ctgttccgta cttgacgaat
    27361 cagtattctt ttcagaagaa ctggttgttc ctgattgttc cggagttgtg actatacttg
    27421 aatccgttgt tgttgaacta tgcacacctt cttcttcgat cgaatcagtc ggtaaagttg
    27481 attcaggaaa ctctggttct ggtgtcactg gctctttttc aggtttctct gactgcaatt
    27541 gttcaggttc tgctggtttt tccgttgttc ctccatttag ctctgtctca acatctggta
    27601 atccttcccc tggcaatgtt tctacaattt ctgacttagg tgtttctaca gtttcatctg
    27661 cataaaccaa tgaactccct aattgaaacg aaaataatga aattcctagt gttgttacga
    27721 ctgctaatgt tgtttttttc atgattattc ctccaatttt ttatgcaatt cgtgttttgt
    27781 tcatagacgc cgcactaata ttatctgaac gataaaagaa ctcccgtccg ttttcttcta
    27841 acccaacata tggattattt ttgaataatt gaatcaatag ttcactggtg ttgtattttg
    27901 gatagtttgc aaaaaaataa tactgtgctt gtcgcccatc cgaaaattca acaagtaatt
    27961 cataacgata ccgcttcaat ccttttcgtt gttttaaagg atctctatac caacttgtcc
    28021 atcgttccat tcgtggatag agatagacac gatacacata gaacaatgcc ataccacata
    28081 ccaccaaaaa taataatcgc cacataaaaa ttcccccttt tcttctacct ttcttgaatc
    28141 atcgaacatt cataccctct atagagattt gaaaaatcca tcatcaattg ttcatataaa
    28201 cgttgttgca aatagcgatt gtttcgaata cataattctt catagccatc aatcacttga
    28261 tcttcttttt catagcatct tgtagctcta tgacgctcag cttgcaagac ataaccaaac
    28321 tctgcaccat atcgttctac tgttaataac tgttgactac gcaataatgc aatataacaa
    28381 tcacaaaaat gttcaaccga catttgattt ttctcacgtt tgtgaaatag gtattcttta
    28441 gaaagcacat tattcgttaa acaatctgat aaatagcgat aatctggcgg tcggttattt
    28501 tcatctgtga atttttccca taataaactg atttttattc gttcagcggc tagatagtct
    28561 tgttcgcgtg gataaaattc atcaatcgca caaagttttt tatcacctga ataaacaaat
    28621 ccatataatg cgaaacgaac tcttcctttt caattggctc tcctaaaaat tccagtgaca
    28681 taccatcttc tccttcctaa aaaaaataaa aaagcagaaa ccgatttggc ttctactttt
    28741 ctttcctttt atagctcttt tttacggaag aatccgcaaa tgacattttt tcaaaaaatc
    28801 aatcggcaca agttcacgaa aacttggagc aattttggat agaatcactt ccccatcttg
    28861 taaaatacaa tcataaaaat catcagttac ttgatactta cgcttcttac aataattgat
    28921 aaaatagtag cgatcatttt ctatttccat tcgttgttct actaagcgca tacgaacatc
    28981 atcatagaaa taagccacaa atcgcccttc aagttcatgt ttttcaatgg gatactcttc
    29041 tggtaacaat ctattttttc gtcgtttata cattgctgga taccttagaa gaagttcatc
    29101 taaacgtctg tcagtatatg gtaactgacg aatcaacgat tctaatcgtt ctttatacag
    29161 atagaataat tggcgcaagc aatagtgtac cgcctctgga ttttctaata ctgttggatc
    29221 acgtgaccaa tacgcaccaa cataaatcat aatttttttc atttagacaa tcacaccagc
    29281 aatactaatg accgatccac aataggtaga acaagtggca tagcgaccaa tttcctctaa
    29341 ttctatctca atctcttcta aagaaagata tgtctgttct tctgttggct ggcgactcat
    29401 ttcccaagtg acagtcacca atgaaatttc ttccataaga ggtttttcgc tgctaagtgc
    29461 tgccacacag ttttcatata tcttcatttt atcgcagcct tttttaaccg ctaaaatcgc
    29521 aggtattggc aatgaaaatc gataaaagcg aatgaattct agccttcctt tccttcggct
    29581 aaattccaac tcttcgagtt cttgttcgta tcccttacga acttctaata agcgtgcaat
    29641 ttcttgccag ttctcttttt cctgataacg tccagctttt accgcttgtt caatataagg
    29701 aactcgaaaa cgaaaataag acaaatcaac catcttttca tttaatgctt cgattgcagc
    29761 ctttaaatcc aacaggcgca gccattctgt tgtcgtatat gcattcatgc aattttccct
    29821 cctgacgttc ccatccattc tagatagttt tgttgctcgt catttagttc ggcattatca
    29881 atcccttctt gtacaaagtc tcttcctaaa atacgatctg cttcttgtaa caactttaat
    29941 gtcggagcta cttgtttcaa aaaccagcgt tctgaacgat ctaatgagaa tttttctggc
    30001 tttactgtaa aatccaattc ttccatcgaa cgaaataatt ttgcccatcg tttatcaaca
    30061 atccgtgcat cactttccag tctatcccaa acaataagtt ttgcattaat taaaccaacg
    30121 ccaatctctc ctaaacgttt cggtgaatca cctaaaataa atgcttctac tgcttgtgtt
    30181 gctttctcat tggctaaccg taactcatat cgattataaa taccatattt cgttaatgct
    30241 tctaattctg aaattttttc tcggttcgca atttcaaaac gtttctcata aaaattaaaa
    30301 aagagagga
//
LOCUS       DACIRY010000026        29913 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.026, whole genome shotgun sequence.
ACCESSION   DACIRY010000026 DACIRY010000000
VERSION     DACIRY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.026"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            283..1152
                     /locus_tag="IYE22_000948"
     CDS             283..1152
                     /locus_tag="IYE22_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ9506176.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1361..2386
                     /locus_tag="IYE22_000949"
     CDS             1361..2386
                     /locus_tag="IYE22_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ9506177.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2425..3375
                     /gene="manA"
                     /locus_tag="IYE22_000950"
     CDS             2425..3375
                     /gene="manA"
                     /locus_tag="IYE22_000950"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ9506178.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3469..5172
                     /locus_tag="IYE22_000951"
     CDS             3469..5172
                     /locus_tag="IYE22_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9506179.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5174..7207
                     /locus_tag="IYE22_000952"
     CDS             5174..7207
                     /locus_tag="IYE22_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ9506180.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7218..7658
                     /locus_tag="IYE22_000953"
     CDS             7218..7658
                     /locus_tag="IYE22_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9506181.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7858..8985)
                     /locus_tag="IYE22_000954"
     CDS             complement(7858..8985)
                     /locus_tag="IYE22_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506182.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8988..9665)
                     /locus_tag="IYE22_000955"
     CDS             complement(8988..9665)
                     /locus_tag="IYE22_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506183.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9929..11695
                     /locus_tag="IYE22_000956"
     CDS             9929..11695
                     /locus_tag="IYE22_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ9506184.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11710..12147
                     /locus_tag="IYE22_000957"
     CDS             11710..12147
                     /locus_tag="IYE22_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9506185.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12160..13311
                     /locus_tag="IYE22_000958"
     CDS             12160..13311
                     /locus_tag="IYE22_000958"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ9506186.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13373..14071)
                     /locus_tag="IYE22_000959"
     CDS             complement(13373..14071)
                     /locus_tag="IYE22_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ9506187.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14064..15416)
                     /locus_tag="IYE22_000960"
     CDS             complement(14064..15416)
                     /locus_tag="IYE22_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ9506188.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15639..17039)
                     /locus_tag="IYE22_000961"
     CDS             complement(15639..17039)
                     /locus_tag="IYE22_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ9506189.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17200..17937
                     /locus_tag="IYE22_000962"
     CDS             17200..17937
                     /locus_tag="IYE22_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ9506190.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18062..18541
                     /locus_tag="IYE22_000963"
     CDS             18062..18541
                     /locus_tag="IYE22_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ9506191.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18538..19305
                     /locus_tag="IYE22_000964"
     CDS             18538..19305
                     /locus_tag="IYE22_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506192.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19535..20104
                     /gene="lepB"
                     /locus_tag="IYE22_000965"
     CDS             19535..20104
                     /gene="lepB"
                     /locus_tag="IYE22_000965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ9506193.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20208..20810)
                     /locus_tag="IYE22_000966"
     CDS             complement(20208..20810)
                     /locus_tag="IYE22_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ9506194.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20916..21385
                     /locus_tag="IYE22_000967"
                     /pseudo
     CDS             20916..21385
                     /locus_tag="IYE22_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21491..22075
                     /locus_tag="IYE22_000968"
     CDS             21491..22075
                     /locus_tag="IYE22_000968"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ9506195.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22078..23151
                     /gene="prfA"
                     /locus_tag="IYE22_000969"
     CDS             22078..23151
                     /gene="prfA"
                     /locus_tag="IYE22_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ9506196.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23144..23983
                     /gene="prmC"
                     /locus_tag="IYE22_000970"
     CDS             23144..23983
                     /gene="prmC"
                     /locus_tag="IYE22_000970"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ9506197.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23976..24155
                     /locus_tag="IYE22_000971"
     CDS             23976..24155
                     /locus_tag="IYE22_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506198.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24177..25202
                     /locus_tag="IYE22_000972"
     CDS             24177..25202
                     /locus_tag="IYE22_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ9506199.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25377..26621
                     /locus_tag="IYE22_000973"
     CDS             25377..26621
                     /locus_tag="IYE22_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ9506200.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26774..27721
                     /locus_tag="IYE22_000974"
     CDS             26774..27721
                     /locus_tag="IYE22_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9506201.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27855..28484
                     /gene="upp"
                     /locus_tag="IYE22_000975"
     CDS             27855..28484
                     /gene="upp"
                     /locus_tag="IYE22_000975"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ9506202.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28623..28865)
                     /locus_tag="IYE22_000976"
     CDS             complement(28623..28865)
                     /locus_tag="IYE22_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506203.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28986..29058
                     /locus_tag="IYE22_000977"
     tRNA            28986..29058
                     /locus_tag="IYE22_000977"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29019..29021,aa:Thr,seq:cgt)
     gene            complement(29177..29815)
                     /locus_tag="IYE22_000978"
     CDS             complement(29177..29815)
                     /locus_tag="IYE22_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ9506204.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10172 a         5277 c         6085 g         8379 t
ORIGIN      
        1 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
       61 aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc
      121 tttttcacaa cctcttttta taagagtttt tactcattaa tttacaaaaa acaagcaaaa
      181 tgcgaatttt ctcacatttt ttttaacaaa atcaaagtta ttgcttgaaa gaagaagaat
      241 ccatcgttaa aataaagctt gacgtagagg ggagcaagaa atatgaaaga ttttcaattg
      301 ggggctgcca cctatttaaa aagtgtagcc attcgggtga aagaccgaga taaaatgatt
      361 gatttttaca agaacgttgt tggctttgat ttgaaaagag aagaaaacga attagccatt
      421 ttaggaacag aagaagctgg aagtgaaatg ttgctgctgg aagaaagtcc tagagcgaat
      481 gatcacatgg gggtgatcaa aaagctgaac cgtttttcat tgattatccc tagcacagaa
      541 gaaatggcag atatcctttg tcgtatccgt aaaatgaatt atccaatcaa agaagcttta
      601 gaagatcatg gacgtatggg gattttgctc gtagacccag aagagaatga attggaaatt
      661 taccatgaag agaataagaa cgaccaaaga gaaattccag aaacaatgga tcaagaagca
      721 ttattaagaa aatctgaaga aaaattcagt aaactatcaa caggttctta ttttgaaaaa
      781 atccatttaa atgttactaa cttaacaaaa gaacgccaat ttcttaatca tgtacttggt
      841 ttgaaggtac ataatgaaga agagggcatc catgtattga atgaaggaga tttccatgtt
      901 ggtttgacag aagctagcgg aggcgcaatt gatttgccta cagatgaagt gattggactt
      961 gattttctga aattcatgat cgatactgaa agcatggaag gactgattgc tcacttgcag
     1021 caactatcag ttgatttctt cttagacaaa aagcacacag tactcacaat ctatgatgct
     1081 ataggaatcg aatggtggtt cgtcagtgag ccaaaagaaa agaataaaac tgacattcga
     1141 tgcgacgctt aactttttct ttcttaacga ttgtcgcatg tctcgtcatt ttatgaacgg
     1201 tcatactatc gcttattcta gagatagaaa gagaaaatag tataccgggg gaaactgcgc
     1261 ggtttttcgc gcagtttttt tgtgtaaaaa aggttgattc ctcatagatt aagctttaag
     1321 cttggactgt aagaatctga tagaaaagca ggagaaaaag atgtatccac acacgaaaca
     1381 gcttattttg aaaaagatgg aagaaggatt ttatccaggt gtagcagtcg ccttcataaa
     1441 agaaaatcaa gtagaaacat ttacagccgg ctttgcccaa gtcgttcctg aaaagcagct
     1501 gatgacagaa gatctgttgt ttgatgcagc ctcactgacg aaagtcgttg caaccacttc
     1561 attagtttta cgtctattag aagatggaaa gatagaactc gatcaaccgc ttcataaata
     1621 tcttcctgaa tttgaaaatc agacaatcac tttgcgtcat ttactgacac atacttctga
     1681 tatcaaaaca tggataccaa acagagataa gctatctcag gaagaattga tcgccgctta
     1741 tttgaagcta gaaccaggtg agcaactagg aaaagaagta aaatatacag atgccggtac
     1801 gattcttctt gggttgatgc tagaggaaat ttaccaagaa actgcagtgg aaatctttca
     1861 agaacaagta ttggaaccat taggaatgat gaacagctta tttcttcctt ttccttccaa
     1921 aaaaatcgtt cccacagaac aactgccttc gggtgaggtt ttgcgtggac aaactcatga
     1981 tccgaaagca agagttttgg ctgaacatgc aggtaacgcg ggtctcttta ttgatttgaa
     2041 agacagcatt acttttgtcc aaatgtattt gaacaaggga aaaatccgta atggtcgttt
     2101 tttagaagaa gagacgattg attctttact gcaagatcaa acaccgaaaa aaaatggcta
     2161 tcgttcttta ggatgggact taaaaaagga tgtagaagac ggtcatgcga ttctttttca
     2221 tactggttat acaggtacgt ttttcttgat tgatataaaa acacaagaag cgttgatatt
     2281 tttgtcaaat cgcgtacatc ctgtcgatca tcgtgcggag tatctcatat gtcgagatga
     2341 aattgttgaa aactttttga aagagaaagc ggtattaccg aaataatgct ataatagcta
     2401 tgaaaaatga gaggagtttg aattatgcag gaacctatgt ttttaaaacc agtttttcaa
     2461 gaaaaaattt ggggaggaag tcgtttacgt tcagtctttg gttttgatat ccctaacgat
     2521 aaaatcggag aagattgggc gatcagtgcg catcctcatg gtgtcagtgt agtagaaaat
     2581 ggcgaattca aagggaaaag attagatgaa ctatggtcag aacacaagga attgtttggc
     2641 catccttcag aaccagtgtt tccattattg atcaagatct tagacgcaga agatgatcta
     2701 tctgtacaag tacatccaga tgatgcctat gggatgaaac atgaaggtga attaggaaaa
     2761 actgaatgct ggtatatcat cgatgctgaa cctggtgcag aaatcatcta tggtcatcat
     2821 gcaaagacaa gagaagaact agctgagatg atcaaagatg ggcgctggga tgaccttttg
     2881 aagaaagtac cagttaaaaa aggtgatttc ttttatgtac caagcggaac gatccatgct
     2941 attggtaaag gaatcatgat tttagaaaca cagcaaagtt ctgacacgac ttaccgcgtg
     3001 tatgactacg acagaaaaga tgcgcaagga caaacaagag agctgcatat ccaacaatcg
     3061 atcgatgtaa caactgtccc tgcgaaaaca ccagaattac aaatcaaaga agtccgaaaa
     3121 gggaattcat cgatcgtcac ttatttagaa actgagtttt tcaatgtata tgagtgggat
     3181 attaaaggaa tcacatcttt caaaaaacaa gccccttata cattgatgac ggtaatcgaa
     3241 ggagcaggag accttgtcgt tgacggaaaa acatatccat tagaaaaagg aacaagctgt
     3301 attattccaa atggcgtttc tgagtgggcg gtccaaggtg aactagcaat cattgcgtct
     3361 gaaccaggca aatagttccc agatagctga aagcagcaga cggtttttcg tttgctgctt
     3421 tttctacata aaatccgtcc atcaataaat agaagtctgg tgaaaaagat ggaatacaaa
     3481 tggaaagcaa acaacagcag tcgcgtgtct atgaaacaac tgagaaaagc tggacatcac
     3541 ttaaatgaaa tgatcatgcc aaatctaagt ctgtttatcg cttggggaat cttgtcatta
     3601 gcaacaaaag gtgtgtctgg cgatttgcac accacattag aagaggtatg ccattggatg
     3661 atccagctgc ttttacccat cttgatcagt tattcaggcg ggaaatcggt agggggacaa
     3721 aaaggggcag tcgccggtgc aattgcttct ttagggttga tcgctcgttc caatgctcca
     3781 caaatcgtag gagcgatgat tatcgggccg cttgccggca tcgtatatga acaatttgtt
     3841 tctagattcc atgtgaaatt caaggctgga tatgaaatgc ttgtcagcaa cctactagta
     3901 ggactgactg gtagcgttat ttgtataatt ggaatcgttt ttattgaacc agttttaaat
     3961 agtgttcact tgttgctcgc ctccgttgtg tcttggctag tcacaatgaa tctgttacca
     4021 cttgtcagtc tgatcttaga acctctaaaa gtactatttt tcaataatgc aatcaatcat
     4081 ggtgtattga cacctttagg aatcgaattc tcacaaacag tcgggtactc ttccttattt
     4141 ttgatggagg caaatcctgg acctggttta gggatcttac tttctatcct ttgttttgca
     4201 gaaaaacagg aaaaagtaaa tgcgagtgga gcattgatga tccatgcgat tggcgggatt
     4261 cacgaaatct attttccatt tgtcttgctg cgaccgtatc tatttttagc tgtaatggct
     4321 ggtggggcaa gcggcacgtt gatatttcaa tggttagata cgggattagc tgcacctgta
     4381 tcgcctgggt cactactcgt gatttttgcc aatgcgcctt atccacaact agctggggta
     4441 agtataggga tagcaatcag tacatttgtg accttcattt gtgcagcgat actattgaaa
     4501 ttaccaaata aagaaaaaga gaacaaaacg gtcaaagaaa ggggagaaat cttggaacca
     4561 tctatcattc attccgtcat ttttgcttgc gactcaggta tgggatcaag tgctatggga
     4621 gccgccctgc tgaaaaagaa attagcagca gaaaaaatcg atctttctgt cggatacagc
     4681 tctatctatg aattgaataa ccaagtaaat cagctggtta tcgtgcaaca tgaactagcg
     4741 ggtcttgcaa gagaaaaagt acctcaaagt caaatctatg tactggatca ctttttggaa
     4801 acagatatct tgctgtcacg aataaaagaa gcgatccaat ttccaaagcg ggaagaaaaa
     4861 aaagagatag cagatatcac agaacaaaaa acgtcgcctg ctaatattgt atttttgtat
     4921 aaaaacaaca gacgcggctc acaaactatg ggcatgacga ttttaagtca attagcaaaa
     4981 aaagcgaaaa aacagtttgt tatctcaaaa gaatcaatag aaaaaatcaa aatccaagaa
     5041 gaaacgattt atatcgcaca aagagaacta gctgtaactg aacagctaga agaaaagatc
     5101 cctcggctag ttctagttga acattgggta gctacgaatg aatacgaaaa actcgtgaag
     5161 ggagaaatat aagatgtatt tatcgcccag agaaaaacaa ttattagcag aatttctcag
     5221 tagcccgtca cctgtatcta tccaaaaaat gatgaattta ttgaaggtca gtaaacggac
     5281 tatttaccgc gaattagata atcttgaagt ctcattgaaa tcggtaaatg cttctttgaa
     5341 aaaagcagct agaggctcat ttgtcattga ggcatcagaa gaagtattag agcaattaaa
     5401 agaaaataca gatgggaaaa gtaatgactt atccactgct aaacgacaac acgcgatttt
     5461 agccgagttg ttactgacga cagagccgtt gagtcttgct catttcatgg aatcatatcg
     5521 catcagtaat accacttttt atgcagatat caaacagttg gaagaaagta ttcgccaact
     5581 gcctttggaa atcattcgga accagggata cgagatcgaa ggaccggaaa aataccgccg
     5641 gcttctgaca gcgaatgtat tagaattaga aatcaatgaa tatgaacttt ttcatagtat
     5701 ttctttcgat gcatcactga attatttttt tcgattcgtt gatccgcaac atctatctct
     5761 tgcacgcaaa gtaatcgggg aagaattgat ccaaaaaaag accaatctaa gtgatagaaa
     5821 gcttgaacat ttggttttga tgctgacatt gacgatggag cgtgtgacga aaaatcattt
     5881 actgacaaat gagacatata atagtctggc aaacaaagaa ttactacaga cagctaaacg
     5941 attgttttca cggatcgctt ctgagacaaa acaactttat ccagtaaatg agatcgtctt
     6001 ttttgcagga ttattaaatg acttcacgaa ctcatttgat cagaatttct ttgaagagac
     6061 gttcgataca cagcttgctt atttagtaaa acaactgatt caagaagtaa gtgaagagac
     6121 gaatgtccat ttttatgaag atgaaacttt gtacaaaatg ctgttgactc atttatctgg
     6181 tgtgttttca agagccgttt tacaagaaga gacactgacc aatccgattt tagaaaaaat
     6241 catggttcaa tacgaggaag ttgcgaatgc aatccgtaaa gcagtagagc ggatttttca
     6301 agaaaaaaaa ttatccgaag aagagatcgc ttatatggtc cttcattttg cgaattcatt
     6361 ggaacgaagt ccgaaagaaa gcaatatcag tattgcagga ttttcgccaa gtggattagc
     6421 ttctactagc atgttggaac tacgattgaa aaggtatttt cccttcatcc aacaaattca
     6481 tttctatcgg attgctgatt taaaaaaaat cgatttacaa gaagaatatg atgttgttat
     6541 ttctacatct attttaccag gatacggtgg aaaatatatt ttagtctccc cgctactgct
     6601 agaagatgaa gtcaagcagc taaaagaagc tttttcgcat attgagaaga aaaaaaggca
     6661 aaaaattacg tataacagca ataacgagtg gatgatagaa gatacttatg aggaaacaat
     6721 gcatttcatg gatcaaatca atcaattact cggtcgtttt tttatccaag aactggataa
     6781 tcctgagacg atttccgaaa cgattcgcag cttatttcct tattttcctg aaggactagt
     6841 aacggatgaa gaggtggttc atcgccggct gcttaagcga tatgaacaag cacctatcgg
     6901 tattcctcat acaaatatgg ggttattcca tacatccagc tcattgattt ctaaaccgat
     6961 cttttgtatt ttcaatttga aacatccgtt gaccattgaa gggatggata gacagccagt
     7021 cgatttgtcc cgtatgttag ttatgctggc gccttcgcca atccaagaaa aagacgggat
     7081 tcttttagga aagatcagtg gagcaatcat catgaatgac ttgaacaccg agatcttccg
     7141 ctcaggaaat aaagaaatcg tttatcagtt gttagcgaaa atcctgattg aagaaataaa
     7201 gaaatgaagg tgaaaagatg atacagctac cagcagaaaa aatattgctc gatcaatcct
     7261 atcaaaattg gaaagaagcg ttgaccgaga tcggccagtg gatgacccaa caagggggaa
     7321 tcaaaccggc atatatccaa tcaatgatcg aaagacaaga aaaagcgagt gtctatatcg
     7381 ggaatttcgt agccttgcca catgggactg acaaagatga agaagtagta gaagaaggaa
     7441 tctatttagt ccaagtgcct gatggtgtgg actttggaac aaaagaggaa ccaaaaatcg
     7501 cgacactctt atttgtagtg gctttaaaaa aagaacatca gttagcggtg ctccaagatt
     7561 tagccttttt ctgttcagac atcgaccaag taatggcgct cagtgatgcg gaaaccattg
     7621 aagaagtcca aatgattttt gaacatggac atatataacc aatgttcaca gtaaaaaaag
     7681 ctgccttgca tcaagtaata acttcaacca agataaaaat aagaggagct atttttgttg
     7741 ggaggttctt attactgcgg atgcaagctt ttgaaaacta tttattctag aaaaccgttt
     7801 atttgaaata ggggacggtg acaaggtttt tgtcaccgcc cctattattt ttgtgtttta
     7861 atagctcttt tttatataga tagtgcccat acctatgaat aataaaacga tactccatat
     7921 accaagtgtc aagataccat tcatgaaagt aacctgttct gaagctagtg gcgagaaaat
     7981 atctcctcct atgatcacat tttgaaaatc aacataagaa gttggcaagt aatgactgat
     8041 tgattctatc catccaatcg aagaaaacaa gcgatactct gctaagaaaa gacaaagaag
     8101 tgtacctaaa agattgacta gagaatgttc aaataaagca gatagaagaa aactgattgt
     8161 acttaaaaag agcacccaca acaacaggaa cagaaagctc tggaaaagat actcatacat
     8221 cggcaggatt cgtatctcct gaccaatgat cgttcctaca ggataacgga agtctcccaa
     8281 accatttttg attattacta gtatagagac gatcaaaaat ggcatcacga gacttagcat
     8341 agctgcacca taataaatgg taaaacggat gctatgtttc tttaccatac tatccggtaa
     8401 tagattgact agagagatta cctgctttct tttttcataa gagatgatgg aagaaagaaa
     8461 caaggcggtt atacataaaa tcaaaaaact agaaatcatt cctgaaaaaa tattttcgta
     8521 gtaatttgct aaagagagat gcgctggcaa gtcaaaaata gaatatcttt ggatctgatg
     8581 ttctttcata taacggagca actcgacacg tttttgttgt tcaatgattg gaattccttg
     8641 tctttgacca gaaatcagtc gatccagtat atctttttca taatgataag ttgcttcgac
     8701 catttgctgg acattccctg tgttttttgc atgtaagatt gcttcgatca gtttgttgct
     8761 tttctctaaa ctttccacaa attctttctc attctcatct tctttcaacg tttcaatcaa
     8821 acgttgcatc tcttcatagt tatgattcag ttcatcgaat tctgtataag caagtttatg
     8881 atcaaaaaaa agtgggaatg caaagagcac gaccaacaat agaggaaata aaaagtttac
     8941 tttgtttttt agtaagagat tgcgttcaaa tgtaagtaaa gacatagtta cacctcatat
     9001 aatttacgat aaatagctaa aatctcttcc atatttgagg attctgtgac gatttctcca
     9061 ttttttagaa aacatgcttt agaacaaatc gattggatat tcgcgatagc gtgggaagaa
     9121 agaagaatcg tcttcccctc tttctgccat tgtctgatga ggctattcaa gatatcaata
     9181 cttcctgggt ctaatccgtt caacggttca tcgaataaca tgacagatga atcactgatc
     9241 aagtaagcag caagcaagac atgttgtttc attcctagag acatttttcc aatcggcaaa
     9301 gtaaggtact cagtcatctt caatcgttgg aaaacagttt ggcattctac tttgctctgc
     9361 caatagtatt tgatcaaagt caaataatca acagccgata gatagtcgga taactgattc
     9421 gttgattcta gaaaaaagaa agaccggcga taatctgtat aagcagtagg agagatgttg
     9481 tgaagggaca attgatcata agttgttggc agcagttggg caatgccatt cagcaaagtt
     9541 gttttgccaa atccgttcgg ggcaatcaaa gcagctactt ctcctgattc tactaaaaga
     9601 ttcaattgtc gaatcacacg ttttttacca cgtttcatgt tcatgttttt gatcagtaat
     9661 gtcatagata ggcctccaat ataaaattta ataacataat agaatggaaa acatctaaat
     9721 taaatgctaa caaaagttat tgtttgcata ttataaaaaa attaaaataa cagttttatt
     9781 taaaattgaa gttttctgat accgaaatct ttttattttg ttgacatact caaacctttg
     9841 gcacaaagtt tcgtgccaaa gtacgactat ttctcttttt agtaagcgga tacaattaga
     9901 gtatcaaaag gagagaggga gagacaaaat ggaacggacg attcccaaaa acaacatttc
     9961 gctaaaagcg agggtacaaa aattaggcag tactttatca agcatggtca tgccaaatat
    10021 tggagcactg attgcctggg gtgtattaac ggctctattt atcccagatg gttatttacc
    10081 aaatgaggct ttcgctacca tggtaagccc aatgctaact tatctgattc ctttattgat
    10141 cggttatact ggcggaaaag taattgctgg tgaccgcgga tcagttgttg gggcaattgc
    10201 aacgatgggt gtgattgtcg gaacagatat tccaatgatg ctaggcgcga tgatcatggg
    10261 acctcttggt ggatatgcca tcaagaaatt tgatcagcta ttccaaaaac ggatcaaatc
    10321 cggttttgaa atgctagtca ataatttttc agcaggtctt atcggttttg gattagcgtt
    10381 attaggtttt tctgcgattg gtccagttgt cgatgcattg actcaagcaa tggcaaaagg
    10441 tgtagagatc attttatctg cccatttgat tccattgaca agtattttta ttgaaccagc
    10501 aaaaatctta tttttgaata atgcaatcaa tcatgggatt ttgacaccat tgggaactga
    10561 acaagtagca gaggctggaa aatctattct attcttgctt gaagcaaatc ctggtccagg
    10621 acttggcgta ttacttgctt ttactttgtt tggcaaagga gcagcaaaat cttcagcacc
    10681 aggagccatg atcattcact ttttaggcgg gatccatgaa atctattttc cttacgttat
    10741 gatgaaaccg ttattattct tatcagttat aggcggagga atgtcaggaa gtttcatttt
    10801 ccaattgatg gatgctggac taagagcgcc agcttctcca gggtcgatca ttgcgattct
    10861 agcgatgaca ccgttcaatt cttaccttcc tgttattttg ggcgtgattg ttgccgcagc
    10921 tgtttcgttt ggtatttctg cagtaatttt gaaagcggat gcgaaagata caaatgaaga
    10981 cttcgaaaaa tctgttcaag agacaaaggc agctaagcaa gcttccaaag gtgcccaagg
    11041 tattcctcaa ggagctacat tctctggcgt acaaaaaatc atttttgctt gtgatgcagg
    11101 aatgggatca agtgcgatgg gcgcttcgat cttgagaaaa aaagtacagg aagcagactt
    11161 gcctcaaacg gtgaccaatc aagcaatcag ccaactgaca gatgactcga atacattgat
    11221 cgttactcag gtcgaactac aagaaagagc aaaacaaaaa gcaccgagcg cgcgttttgt
    11281 ctcagtagaa aactttttga attcacctcg atatgatgaa atcattcaag aattaagcag
    11341 caagcagaaa acatcagttt ctgaaattga gaatgagcca gcagaagagt ttctttcagc
    11401 aaacatcaaa caaatcaaag agattctact tgtccacgat gatcgttccg gatcagcaac
    11461 aatggggatg accgtgctca aagatatatt agacaagaac catttaacta tcccgttaag
    11521 aaaagtcaat atccaagatt taaaagatga acctgattca ctgatcgtag ccaaagaaag
    11581 tttagcggaa aaagtacaga tggctgcacc tcaatccatt catttatcag tcaacagcat
    11641 ggtcaatccg caaaaatacg aatcgattgc acaatatttg aaacagacag cttaagggag
    11701 gaaaacagca tgcaaagttt gccattcgac atgatcgagc tcaatcgatc ttttaaaacc
    11761 aaagaagaag cgatccgcta ttgcggtcag atgcttgtag aagcagggtg cgttgatgag
    11821 gaatatatcg atgccatggt agatagagac aaaatgttat ctgtttatat ggggaatttt
    11881 atcgcgattc ctcatggaac agatgaagca aaaaaatatg tgaaaaaatc cggcatctgt
    11941 gtcgttcaag taccagatgg tgtagatttt ggtaccgaag aagaagaaaa gattgcgact
    12001 gttttgttcg gtattgcagg agtcggagat gagcatttgc aacttgtaca gcaaatcgca
    12061 ctttattgca gtgatatgga caatgttgtc cagctagcag acgcgctcag caaagaagaa
    12121 gtaacggaaa atctagcaat tgcttaagga gagagaaaaa tgagagcagt acattttggt
    12181 gcaggaaata tcggtcgagg atttattgga gaggttttgg cagctaatgg atttgagatt
    12241 actttcgtag atgtgaatca cacgatcatt gatgctttaa atgaaagaaa agaatacgaa
    12301 atcgaattag cagatgaatc aaaagaaaaa atcactgtaa aaaatgtcaa aggaatcaac
    12361 aatcaaacaa atccagaaaa agtaatccaa gaaatcgctg aagcagattt agttactacc
    12421 gccattggcc cgaatatctt accttttatt gctgagttga ttgcaaaagg tatccaagaa
    12481 agagaaaagg aaggaagcac aacatcttta gatatcattg cctgtgaaaa tatgattggc
    12541 ggttcagcat ttctagaaaa agaagtgtat aaatatcttc cggaaacatc atttactgac
    12601 aaatatatcg gctttccaaa tgcagcagtc gatcgaatcg tcccattaca acaccatgaa
    12661 gatcctttat tcgtccaagt ggaacctttc aaagaatggg tcattgacga tagccaaaga
    12721 aaaaataaaa aaatccaatt aaaaggggtt ttatatgtag atgatctaga accatatatc
    12781 gaacgcaaac tatttagtgt caatactggt catgcgactg tcgcttatac aggtgccttg
    12841 ttaggttacc agaccattga tgaagcaatg caagatgcgt tagttgtggc acaattaaaa
    12901 tcagttttgc aggaaacagg aagtttgctg attgccaaat gggggtttga tgcagagcag
    12961 catcatgcgt atattgaaaa aatcattcat cgcttccaaa acaaaaatat ctctgatgcg
    13021 atcacacgtg tcgctcggac accattacgt aagttaggtt atcaagaaag atttacacgt
    13081 ccagtgagag aactacaaga acataacttg acttgcccac atttgacggc aacaatgggg
    13141 attattttta attactatga tcctgaagat gaacaaagcc gtcaattgca tgaaatgaag
    13201 atccatgaaa acttggaaca attgattcaa gaagtcacag gaatcaatga tccgaaaaca
    13261 atcgggaata tcaagcaaaa cgtgaaccgt tatgccaagc aagtcgctta aaataggata
    13321 cgaaaaaggg gaatgtgacg aaatttttgt cacattcccc tttttcgaat aactaggctg
    13381 tttgtccttc aagatagggc agatccaatt ctttaccgta acgttgttct aaagctaggc
    13441 ggatcaaacg agctgccagg ttctcatttc ttgcaaggat aggtccatgg aaataagaac
    13501 cgaagacatt tcggtaaatg acaccttcag attggtcttc accattattt ccttgacctt
    13561 gtaccacttt ccccaaaggt cgttcacctt cacctaagaa agtccgacca ttgtggtttt
    13621 caaatccata ataagtttca ttgaattctt cgttatggat aacgatatca ccgataaacc
    13681 ggttattttc ctgacttaac gtgtagtgat ccagtgcact gattccttgg atcttttctc
    13741 cattagctcc catgtagtag tgacctagca attggtagcc accacaaatg gctaacatga
    13801 cgccatcatt ttcgatatag ttgatcaaag aatctttctt tttctggatg tcttttgaaa
    13861 tgattaattg ctcaaagtct tgaccaccgc caaagaaaac caagtcatat ttatcagcat
    13921 caaaaggttc atgtatacta acgatctccg tatggcaaga aacccccatt ttttctgcta
    13981 tatatttcaa catcaaaagg tttccgttat caccatatgt gttcatcaaa tttccataaa
    14041 ggtgggcgat attgagttca taattagcca cggttcattc ctcctttgat aaatccttga
    14101 gcagctagtt ctttacgaag ctgcaataca gcagtgtaag tagctaaaat atagacatgc
    14161 tcagttggca attctttgat tttttcgatt acttctggta attcttttgt ttctgtcaat
    14221 ttattttctg gaatcccggc aacttttaga cgtaaagtca tatctgtatg acggtctcct
    14281 ccagaaataa cagctgggat atccatatcg gcaaagtttt caaaattacc atcccaaatc
    14341 cagctgacat cgattccgtc tgcgtaattg gcatttaata aagccactaa agaaaatgga
    14401 taaggagcta gaccaatcat atcgattact tgattgatac ctacaggatt tttgacaagt
    14461 actaatgtac attttttccc attgatattg atgacttctt gtcggccgaa tactttctcg
    14521 tcatatccta aaccttgttt gattttatca ggatcgacat tgaagtactc tgccactgca
    14581 gtagctgcta aagcattgta gacattatac ataccgccaa cttcgattcc gtattcatga
    14641 ccatcgatca cgaaatctgc tgacacattg tccatccgta ccatatcagt taatcgataa
    14701 tccaattcag gacgtttgaa gtcacaattt ggacagtaat atttccctaa gtttgcataa
    14761 gtgatcattt tataatgcaa gatgtgatga caatttggac ataagacacc gtctgtattg
    14821 taatgagcca tttgttcacg atcttcttca tggtcaaagc cataatattt tctaggattg
    14881 actgtttcga tagaattgaa gattggtgaa tcaccgttac tgataatcgt tgcatttggt
    14941 gactttgcgg caccgtctac aatcatttta taggtcgtat agatttctcc ataccgatcc
    15001 atttgatcac ggaaaatatt tgtaaataag aacagttctg gtttcatata ttctgtgact
    15061 ttgctcaaac ttgcttcatc tatttctagg acagcaaact ttttctgtcc tttttttggt
    15121 tttgcctgca agaaagtcga tacaattccc tgtaccatat tcgcaccagt agggttggtt
    15181 agaacttcat cgaattgctg gcgcaaaata ttgactgtta aagcggtagt caatgtttta
    15241 ccgtttgttc cggtgacaac gacgacttgg tagtcttttg ccaaactatc tagtatatgt
    15301 ggatcgactt tcagcgctag tttacctggt aaacttgatc cgcctttaaa aaaagttttt
    15361 aatgcccatt gcgacgtttt tccaactgca atcgcaaaat gactgcgtat tcccattatg
    15421 taacctccaa aaacgatttg ctgacgaata aattcaacta atcataccat atgttgaaaa
    15481 aaaggttaaa ttgcttttct tattcttaag aaaaggttac ggctgaaagt gcatgaaatc
    15541 ggacaaaatc gcatgattta acaaaaaaac agcttgaaac aaaatgattg tttcaagctg
    15601 ctgtgatttt attattccct cttacctata ttcccatttt aaccgataat aattttctcg
    15661 cttggatatt catagcctag atctcgtgtt tcttttggca cgaataacat caatgtatat
    15721 agcattccga tccgcccaat aaacatcagt gtagcaatcg tgattttgcc cacaacagac
    15781 aagtctccgg taatccctaa agataaacca gttgttccaa acgcagaagt tacttcgatg
    15841 atgatcgaga tcagcgtttg ttcttctgta gcagataaaa aaacgataca aaagaaacac
    15901 ataccaagag aaagcataaa gacaacgact gattttcgca catcatcttg atcgattctc
    15961 cgtccaaaaa tattgatgtt atcttcactt ttgaggaagg aatatagata caaaccgatg
    16021 attgctacag tagttgttcg aatcccgccg cctacagaac taggactgca tccgataaac
    16081 atcagtaaag aaaaaatgat cagtgttgtg atttgaaaat cacccaaatc gtgaatctgc
    16141 agaccagcat tacgagtcgt aatcgaataa aacatcgaat tgatccattt gacactcata
    16201 ttggaatctt ggaataaatg gtccttttcc aatagataaa tcaagacagt tcccgaaaca
    16261 aacaagataa caaaagctaa cacagctaat ttggtaaaca aggaaaaacg aaacggaagc
    16321 ttcgcattgg aacgtttgta taatagccat tcccggcact ccatcaatac aggaaaacca
    16381 attcctccga caaaaatcaa aaacatgatc aggatcaaga aaaaatagtc atgtgcaaag
    16441 ggcttgatgg aatccccagt gacgtcaaac ccagagttcg tgacagccga tatcgcctga
    16501 taaaaaccgt aaaaaacggc atcacgccaa cgatcaaaat agcctctgta ataaaaataa
    16561 atagaaaaga atgtcccaaa aagtaattga aaccagatta aaatagcaaa agtgatccga
    16621 atcagtcgga cgataccaga caaacgcggt tgattcatat ccgtcatgat cagctgtcgc
    16681 tgtttcagtg tgatccgtcg tttagaaaaa atgacaaaag cagtagaaat catcatgatc
    16741 cccaaaccac caacttgaaa taatatttcc agcagtataa taccgttatc attgaacaca
    16801 gaattgatat caaaagtcga tagtccagtc acactcactg tactgattgc catgaacagc
    16861 atgtccacaa aagaaacatg gctatctggt tcgcgaaaaa tcggcatata aaatagaacg
    16921 agtgataaga ccgtcatcaa tatataataa aaaacgatga tttggatcga tgagaaatga
    16981 ttggcagcaa acttctgtcc tcgccgctgc agccttcgaa agaataaatc aacatgcaca
    17041 gtactatcct cctgacaata gtcgtttcta ccagtataca aggggaatta ggaatactca
    17101 aagaaattat caaacagata acaatacagc tttcgattaa aatagaaatg gcttataata
    17161 aggaaaatcc tagaaaaaag aaggaggagg aataacaaat tgattaatgc tcaacaaatc
    17221 gtagaaaaaa tgaaaaatca aaaaataaat tatgatacag ttctgaaaaa aatgatcatg
    17281 caatgggaga aagaagaagt aaagcctaag atattaatac atacatgctg tgctccttgc
    17341 agtacgcaca cattggaatt catgtctcaa tatgcagaga tcacgttatt tttttcaaat
    17401 tcgaatattc atcctaaaag tgagtatttg cgaagattac atgaacaaaa acgatttgta
    17461 gaccaattta atgagaaaac tgaccatcat gttgcgctaa tcgttgatga ataccgtcca
    17521 aatgaattca ccaaaatggt tttaacaaac catcttgaaa acgaaaaaga aggtggtgct
    17581 cgctgcagtg catgcttcaa tatgcgatta gatcttgttg cgcaaaaagc gcaagaacta
    17641 ggatacgatt actttggaag tgccctgacc atctcgccaa agaaaaatgc agctttgatc
    17701 aatcagatcg gaatggatat ccaaaagatt tacgggacac aatacttgcc aagtgatttc
    17761 aagaaaaaca aaggctatga acgctctttg caaatgtgca aagagtatga tatttatcgc
    17821 caatgttatt gtggttgcgt gttcgctgcc aagcagcaag gatgcgatct gaaagaaatc
    17881 aatcgggaag caaaagctta cttaaaaaca tcaaccgtag tttttgagga aaattaaaga
    17941 aatctttcgg aaaaacgtaa gaaaaaaata aagaatttat ggtagaaata aggtagactg
    18001 tataatttgt ccgtagtaaa gtaaggaaac aagaataaat ttcaatgaag gaagagataa
    18061 gatgcgaaaa gtagcaccta ttttgaagcc aatcgctgta ttaacaatct cgacgattgt
    18121 cgcgactact acaggtattg tgaatagttc cagctaccgt gcaccaagtt caagttctag
    18181 tactgtagaa gaaacaacac caactacttc agtctcgaca tctgaaccaa caacaacgga
    18241 aagtttgaat gaaaccacta cgacaagtac atgggacgat acaacaacta gttcgtcgta
    18301 tgatgagaca tatacaactg acagctcaag cgaaacaagt aatacggtac cagatggcac
    18361 tccatcggat tcaaccaatc caagtacaag tccggataca agtgctagct ccgaaacgat
    18421 accaagcagt acgactgtag atgataacac gcaaaatgat gatgcagcac gtacagcaca
    18481 gcaaaaccaa gattcaggaa atacccaaac gtataaccag caagcaggtg attctcaatg
    18541 acatttttac tagaccttat caacggggtg cataaattcc ttggctattt agacatcagt
    18601 cctaaatatc ttaaccgtgg atatacgatt ttaagtgtga ttcccacgct ttatttattg
    18661 agaattgttt atggactttg gcaaaaccaa aactatcttc aatttttctt atatggactt
    18721 gcatttctag tgcttgttta ttttactgtt ttaaatgtat tttattactt tttagataaa
    18781 aacacgaaag ccgatgtcac tcagcttttt gtgaagtatt tacctgatga agcgttcaac
    18841 atccagacag aagaatcgat agataatgga aattcgatcg ataaagtgaa tacacaagag
    18901 gtcatggttt cttatgacga agattatcaa ttgaaattag ccgagaatat gcggtatttg
    18961 attggaaatg gtgagatcaa aacaaatgaa ctcggtcgca tggatggatt tctggtcgat
    19021 agaaatacat tgtatcctta ttactatgtc aagaaaacag gagagaaaga gtatgttcta
    19081 aatattggac gaagctactc ggacatggaa aaagtaggga ccattcgact tgactcaaca
    19141 gaagacacat tggaacctgt cggattgttc atcgtcggcg gtgattttgt caaagacggg
    19201 ttacgctatc atgagcctta tcgtctgaaa ttgattgcaa aaaaggcaca gtcgcaggaa
    19261 gaaccagtgg tttatagtcg tagccaaaga agaagacatt cataatgtga tgaaacgccg
    19321 tgtatcagtg gttgcgataa aaacgtttat tcggaataag ggggatgtga cacgtctttt
    19381 gtcacgttcc ttttttatat aacgagaaag ataagacgaa cacatcactt attctttcct
    19441 ttcatttata aacattgtag aataaagaaa gtacaacgaa gtattgtgaa aaaacgtaat
    19501 aaataacgag gaaaatacaa gtaggaggta agctgtgaaa gaatttttga aaaattgggg
    19561 gattttgatc cttgtattag cggctatttt attagctaga gtgtacgtgt ttacaccagt
    19621 tactgtaaac gggcattcga tggaccctac attaagtgat ggacaacgat tgatttcatc
    19681 taagatctca aactatgaac gaatggatat tattactaca aaggaaccgg gggatgaaga
    19741 acggatgatt gttaaacgga tcatcggtat gcctggagat acagtcaaga tggaaaatga
    19801 ccagttgacc attaatggca aaaagtatga tgaaccttat ctggatgagt tcaaaaaaga
    19861 attttcagaa gataagttac aaggagaata tgcttatagc tcaggattcc aagcacaagc
    19921 agaaagttca tccaccttta cgagtgactt tgaatatact gtgccaaaag gaaagtatct
    19981 tgttctaggc gataaccgct tgatttcaaa agacagccga atgtttgggt tagtcgacaa
    20041 agatatgatc caaggaaaag ttgttttccg ttattggcct ctttcagaaa ttaaaatcat
    20101 gtgatgaaaa aataaaaaga agctgtgaca tgttttttgt cacagcttct ttttccgaaa
    20161 attccttctt atttcttgga gttgctcact ttttttgcaa tctcatttta agctttttgg
    20221 ctgatggata agaaaagcga agcgtttttg ttcataaata gcttttcccc acgatggaag
    20281 gtatgactag tagcagagac aaagcccgtt ttttccaatc gattattcaa ttcttcttgg
    20341 ctaaagccat tatggacttt cggatgacta atttgttcat tcttgtcaaa gtctacgatg
    20401 atcaattgac cgccaggagc taaaatttca taaagttttg taagaatatt ctccgtatca
    20461 ggaatgtgta aaagtacaag tgatagaagg atgagattag cttggacagc aggaaattcg
    20521 acagaagcat cagcgtggat cgttcggaca tttttcagat ctgctgcttg gatcttttct
    20581 tctgccattt tcagcatagt ttctgaagca tcggcaatga tcagttcttt gaatctttcc
    20641 gcaagcggta aactaacaag cccagttccg cctccgtaat ccagcaggac tttttggtgg
    20701 ctgtcgcgcg gtagataagt agttagttct tcgcgaataa tcttggctaa tgcttgacgg
    20761 tcaggggaat cataatggtt agcgatggaa tcaaaaatat tttttgtcat caaatagctc
    20821 cttacgtaaa actttgaaat tttaacttat tatagcacgt gaaaagaaag aaagcatgct
    20881 atactcaatt caaaaaggga gaatctagaa agaatatgcc gatatcgttt gtaaaagata
    20941 gagaggaaaa aggaaagtgt gtccgagaaa tattactaga cttgcctgag tggtttgggc
    21001 ttcctgaatc aacggaaaaa tatatcgagg aatcaagtaa actaccttta tggtgtgaaa
    21061 aaagaaaaga agaatattta ggctttatca ctctttcaca gacaagtgaa gatactgccg
    21121 aaatctattc tattgtatgg gaataaaaaa agactatcat caccaaggat taggaacaaa
    21181 actttttaaa gaagcagaag gttacgcagc aaaacattac aaatatttgc aagtgaagac
    21241 tgtagacgag gggcactact ctatatatga tcaaactatt tgtttctacg aatcattagg
    21301 attttcaagg ttggaagttt ttccaaattt atgggatgaa tggaacccat gtttagttct
    21361 tgtcaaaaaa ttggaacaaa agtgagcaat agatttaata ctaatctgtg cttgatgaaa
    21421 attctcactg taaaaaatgc tgaaaatcaa gtataatacc ataggaacaa actggaaggt
    21481 gaaaaagagc atggcacaac tattttttaa gtatggcgca atgaatagcg gcaagacgat
    21541 cgagatccta aaagtcgcac ataattatga agaacaaaat aagccagttg tcttaatgac
    21601 aagcggtatt gatacaagag atggtttagg cgttgtttca agccgaatcg gactgaaacg
    21661 taatgcgatg ccgatatttg aagaaacaga cgtatttgac gtaattcaga aaatggaaag
    21721 caaaccctat tgtgtgctga tcgatgaagc gcaattctta aaaaagaaac atgtcattgc
    21781 ttttacgaga attgtggacg aattggatat tccggttatg gcatttggtt tgaaaaatga
    21841 ttttcgaaac gagttatttg aaggatcaaa atatttattg ctttatgcag acaaaatcga
    21901 agaaatgaaa acaatctgtt ggttctgcca caaaaaagca atcatgaatt tacattatat
    21961 tgatggaaac cctgtttatg aaggcgatca agtgcaaatt ggcggaaatg aagcttatta
    22021 ccccgtttgt cgaaaacatt actttcaccc atcaatgatt acagaaggag aataagcatg
    22081 tacgatcaat tacaagcaat tgaagaccgt tatgaagagc ttggagaact tttaagtgac
    22141 ccagaagtga tttctgatac aaagcgtttt atgcagcttt caaaagaaga agcaaacacg
    22201 cgcgaaacag tcgaagttta tcgtcgttat aaagaagtag tacaaggaat caccgataca
    22261 gaagaactgc ttggtgaaaa actggatgat gaaatggctg aactagcaaa agaagaatta
    22321 tctgaattaa aaaaagaaaa agaagtctta gaagaaaaaa tcaagatctt gcttttacca
    22381 aaagatccaa atgacgataa aaatatcatc atggaaatcc gtggagcagc aggcggagac
    22441 gaagcagcat tattcgctgg cgatcttttc aatatgtacc aaaagtatgc tgaagcacaa
    22501 ggctggaaaa cagaagtgat ggaagctaac gttaccggaa tcggtggata taaagaagtg
    22561 atcatgatga tcaccggaga caacgtatat tcgaaattaa aatatgaaag tggtgcgcac
    22621 cgcgtccagc gtgttccttc aactgaatcg caagggcgta tccatacttc caccgcaaca
    22681 gttgtcgtga tgccagaagc tgaagaagta gagatcgata ttgaagacaa agatatccgt
    22741 acagatatct atcatgcttc aggtgctggc ggacagcacg tcaataaaac agcatctgct
    22801 gtacgtttga ctcacttacc aacagggatc gttgttgcta tgcaagatga acgttctcaa
    22861 atcaagaacc gtgaaaaagc aatgaaaatc ttgcgtgctc gtgtgtacga taagatccag
    22921 caagaagcac aaagcgagta tgatgccaat cgaaaatcag cagtcggaac tggggatcgt
    22981 tcagaacgga tccggacgta taatttcccg caaaatcgtg tgactgatca tcgtatcgga
    23041 ttaactatcc aaaaattaga tcagatttta gcagggaaat tagatgagat cgttgatgca
    23101 ttgatcatgt atgatcaaac atcaaaactg gaagctatgc aaaatgggta aaatgaccta
    23161 tcgagaagtc ctgtcacggg cttcttcttt tttagaggat catgggaaag aagcatatag
    23221 tatccaattt ttatttttgg aacgaaaaca atggaaaaaa cttgattggc tccttcatat
    23281 gaacgaagag atttcagaag aagaacagcg gttgatcgaa accgatctgc gacttctgct
    23341 tgcagatcat ccacctcagt atttgctagg atatgcagat ttttatgatc atcggttaaa
    23401 ggtaactgaa gcaacactga tccctcgacc agaaacagag gagttagtcg aatggtgttt
    23461 agacgagacg cctggtgtac cgttagaagt gatagatatt gggacaggga ctggagcaat
    23521 tgctattagt ttgaaagcag caagaaaaaa ttggcatata tctgctgtgg atctttcaga
    23581 agaagcatta gaagtagcga aagaaaatgc tcagaaagaa ggaacgaaaa tcagttttta
    23641 tcatggagat acattaggac cagtaatgga tcagcaattt gatgtgatta tttctaatcc
    23701 gccgtacatc agtcggaatg aatgggaact gatggatgaa agtgtccggt cattcgaacc
    23761 taaaatggcc ctttttgctg agaacgatgg attagctatt tatgaaaaaa tagcagttga
    23821 agcaccaagc gtactaaaat caaatggaaa gattttttta gaaatcggat ttcgccaagg
    23881 tgaagcagta aaaaaaatat ttcagcaagc atttccagat aaaaaagtgg cgataaaaaa
    23941 ggatcttttt ggcaatgaac gaatgatcca agtttatggt tgaagttgtg gatgaaaaat
    24001 tttttatttg tttttttatt ataaaaaaac acttatttga caaggatgta aacgactttt
    24061 tggtttatca acaagtttat ccactattga caaatagatt atccacaaac agtggaaaac
    24121 ttgaggataa actcagacaa aataagaatg tatgaaaaag gggttgtgga taaactgtgg
    24181 aaacgaaacg atatgatgaa acagaattgc aagaggcagc cgaagcactg aaagaaggag
    24241 aacttgttgc ttttcctacc gaaacagtat acggattggg cgccaacgct cttttaccaa
    24301 acgctgtcaa gaaagtgttt tctgtaaaag ggagaccgca agataatcca ttaatcgtcc
    24361 atgtcgcctc ttttgaacaa gtaaaagagt atgtggacaa ctttcatccg gcagcagaga
    24421 agattgtgaa gaacttctgg cctggaccat taactttgat atttaagatc aaaaaagaca
    24481 ctttgccctc agttgtgacg ggaggattat ccacagctgc atttcgtata ccggataaca
    24541 aaaaaacact agaagtaatc gaactttccg gtgttccgct tgtcggtcca agtgcaaata
    24601 cgtcgggaaa accaagtcca acgactgcag atcatgtata tcatgatcta caaggaaaaa
    24661 ttaccgggat tattgatgat ggagctacgc gaattggtgt agaatccact gttcttgatt
    24721 taagtgatcc aacagccatg cctatgattt tacgaccagg tgcagtaacg aaagaacaaa
    24781 tcgaagcagt catcgaatct cctgttgcta ttgatcagca tcttgtaaaa gaaaatgaaa
    24841 caccaaaagc ccctgggatg aaatacaaac actactcacc cgatacacgt gttctgatgg
    24901 tcagagatgg cgattggtca actgcagttc aatgggcaaa aaataagaag atccgtgcag
    24961 gtgtcatagc aagccccgaa attgcggatc aagtgcggac agatactgca gctgtttata
    25021 tgtataacga caactcagta gaagcggcgg ctaaaggatt gtttgcaggt ctaagaggat
    25081 tagatgaacc aacacttgga ttagatttga tttttgtaca agtgtatcca gaaactggat
    25141 tggggaatgc ttacatgaat cgcttgaaaa aagcagcagg acaaaattat tttgaaaaat
    25201 gatcaattgg ttattgtcgc attcttttag tatgatacaa tgctatcaag taaatcttct
    25261 atcagtgaac gaaaaactaa gcgtaactaa tcgtaataga agagatacga atatttggca
    25321 atttgttttt gatgggaaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg
    25381 tagattacaa aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac
    25441 aagaacataa tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg
    25501 cacaaggaag tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg
    25561 gtgggtgtga atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat
    25621 tcggagcgaa atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt
    25681 atctcgcttt ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac
    25741 atctaacaca tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg
    25801 gtgttgatcc gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac
    25861 atcaaccaaa attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga
    25921 aattcagaga aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg
    25981 caggattagt agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga
    26041 ctaccactca taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag
    26101 cattagcaaa aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc
    26161 atgtgattgc gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat
    26221 atagtgaaca aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg
    26281 gaaccagagt catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt
    26341 taggtataaa tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata
    26401 aaaactcgat tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca
    26461 cgccagcaat cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag
    26521 ttgtcaaagc attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg
    26581 tacgcgaatt aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt
    26641 aaacagaaaa gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga
    26701 caatatcggt gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca
    26761 gggaggaaaa gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga
    26821 tgcaaatgcg cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact
    26881 agaagaagta caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt
    26941 ggatactggc gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga
    27001 taaattacca gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac
    27061 ccatgataaa ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga
    27121 atatagtcga tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt
    27181 gcaacagctg aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc
    27241 aggagattca gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa
    27301 gggcctccca atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac
    27361 agattcctat catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt
    27421 ttgggaccaa tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag
    27481 agcaaatata aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga
    27541 tgtattaaga gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt
    27601 tacacaagta cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca
    27661 gacaaaggca acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata
    27721 agctttgctt gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc
    27781 gagtttgact agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa
    27841 ggagagctta aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac
    27901 taactattat tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa
    27961 tcgctatgtt gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg
    28021 aaacaccgat cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc
    28081 caatcttgcg cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta
    28141 aagtgggaca tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg
    28201 tcaaacttcc agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa
    28261 caggcggatc agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta
    28321 aatttgtttg tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg
    28381 atattgacat ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc
    28441 ctggactagg ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg
    28501 gtgtgacaca acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag
    28561 ctttttcaca gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa
    28621 attcaaatct tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc
    28681 aagttcgcat tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt
    28741 gaaatcgttc cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg
    28801 aatttttcac caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt
    28861 ttcataaaac ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt
    28921 aaactaattt tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg
    28981 ataaggccag cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt
    29041 cgatccctat agctggcatt aaaagagaag acagatatat caaggatttt taatccattg
    29101 atacatctgt ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta
    29161 ttatttctat aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata
    29221 tcacatgaac tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata
    29281 acaatcttgc caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat
    29341 tatcataagt actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag
    29401 gaatgctgaa cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa
    29461 gtatcatgat aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc
    29521 ctgttaaacc ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta
    29581 cattgataaa aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt
    29641 ttttcgtatc ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag
    29701 aaaaaatgct aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa
    29761 tagtgcccaa tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa
    29821 taactcctac tattcagttt ttgaagatag agtcagtata catgaagagt taaaataggc
    29881 aatgtttttt tatggatatt tgtattgcta aaa
//
LOCUS       DACIRY010000027        29638 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.027, whole genome shotgun sequence.
ACCESSION   DACIRY010000027 DACIRY010000000
VERSION     DACIRY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.027"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            174..575
                     /locus_tag="IYE22_000979"
     CDS             174..575
                     /locus_tag="IYE22_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ9506205.1"
                     /translation="MNYSPSFQPDSVRTPEDFQRFLEREFYASNRPTIILSYSLSLGL
                     VLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESANLMLNVMGIGSMAAAVVGFIHFD
                     FILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            726..1892
                     /locus_tag="IYE22_000980"
     CDS             726..1892
                     /locus_tag="IYE22_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9506206.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            2122..2769
                     /locus_tag="IYE22_000981"
     CDS             2122..2769
                     /locus_tag="IYE22_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ9506207.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSETSTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            2853..3797
                     /locus_tag="IYE22_000982"
     CDS             2853..3797
                     /locus_tag="IYE22_000982"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ9506208.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            3932..5383
                     /locus_tag="IYE22_000983"
     CDS             3932..5383
                     /locus_tag="IYE22_000983"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ9506209.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            5404..6240
                     /locus_tag="IYE22_000984"
     CDS             5404..6240
                     /locus_tag="IYE22_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ9506210.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            6240..6899
                     /locus_tag="IYE22_000985"
     CDS             6240..6899
                     /locus_tag="IYE22_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ9506211.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            7025..7714
                     /locus_tag="IYE22_000986"
     CDS             7025..7714
                     /locus_tag="IYE22_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506212.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            7720..8676
                     /locus_tag="IYE22_000987"
     CDS             7720..8676
                     /locus_tag="IYE22_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ9506213.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            8720..9232
                     /locus_tag="IYE22_000988"
     CDS             8720..9232
                     /locus_tag="IYE22_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9506214.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(9488..10036)
                     /locus_tag="IYE22_000989"
     CDS             complement(9488..10036)
                     /locus_tag="IYE22_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9506215.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(10125..11306)
                     /locus_tag="IYE22_000990"
     CDS             complement(10125..11306)
                     /locus_tag="IYE22_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ9506216.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(11542..13050)
                     /locus_tag="IYE22_000991"
     CDS             complement(11542..13050)
                     /locus_tag="IYE22_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ9506217.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            13209..13400
                     /locus_tag="IYE22_000992"
     CDS             13209..13400
                     /locus_tag="IYE22_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506218.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            13603..14520
                     /locus_tag="IYE22_000993"
     CDS             13603..14520
                     /locus_tag="IYE22_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ9506219.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            14513..15586
                     /locus_tag="IYE22_000994"
     CDS             14513..15586
                     /locus_tag="IYE22_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506220.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            15734..16252
                     /locus_tag="IYE22_000995"
     CDS             15734..16252
                     /locus_tag="IYE22_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506221.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            16233..16625
                     /locus_tag="IYE22_000996"
     CDS             16233..16625
                     /locus_tag="IYE22_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ9506222.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            16701..17636
                     /locus_tag="IYE22_000997"
     CDS             16701..17636
                     /locus_tag="IYE22_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ9506223.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            17769..18548
                     /locus_tag="IYE22_000998"
     CDS             17769..18548
                     /locus_tag="IYE22_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ9506224.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            18541..18858
                     /locus_tag="IYE22_000999"
     CDS             18541..18858
                     /locus_tag="IYE22_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ9506225.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            19102..20712
                     /locus_tag="IYE22_001000"
     CDS             19102..20712
                     /locus_tag="IYE22_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ9506226.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            20869..21717
                     /locus_tag="IYE22_001001"
     CDS             20869..21717
                     /locus_tag="IYE22_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9506227.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            21948..22427
                     /locus_tag="IYE22_001002"
     CDS             21948..22427
                     /locus_tag="IYE22_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506228.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            22460..24688
                     /locus_tag="IYE22_001003"
     CDS             22460..24688
                     /locus_tag="IYE22_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ9506229.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            24817..26460
                     /locus_tag="IYE22_001004"
     CDS             24817..26460
                     /locus_tag="IYE22_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ9506230.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(26533..27078)
                     /locus_tag="IYE22_001005"
     CDS             complement(26533..27078)
                     /locus_tag="IYE22_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ9506231.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(27101..27586)
                     /locus_tag="IYE22_001006"
     CDS             complement(27101..27586)
                     /locus_tag="IYE22_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9506232.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(27576..28388)
                     /locus_tag="IYE22_001007"
     CDS             complement(27576..28388)
                     /locus_tag="IYE22_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ9506233.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(28527..>29638)
                     /locus_tag="IYE22_001008"
     CDS             complement(28527..>29638)
                     /locus_tag="IYE22_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ9506234.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9735 a         5365 c         5905 g         8633 t
ORIGIN      
        1 tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct gaaattttgg
       61 taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt gattttgaga
      121 aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat acgatgaatt
      181 atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa cgatttttag
      241 aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt ttaagtcttg
      301 gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt ttcttgtgga
      361 tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca aatttgatgc
      421 tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc cactttgact
      481 ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta tgggtttttg
      541 tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa
      601 tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa aaattaaact
      661 tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt ttaaggagga
      721 aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc tcgctccctg
      781 tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac actcctcccc
      841 aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt aacgcttctt
      901 tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt atccaagcat
      961 tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat tatctaaaat
     1021 ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc catgcgcatt
     1081 atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact tattcttgtg
     1141 cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa gtccatcata
     1201 tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt tgtggctggg
     1261 gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg atcacactaa
     1321 gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta gaagataaat
     1381 gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat catatcacaa
     1441 gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc aaagtggtaa
     1501 aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga caacaaggcg
     1561 gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc cctggtgtta
     1621 ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg gaaagcttac
     1681 gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc
     1741 aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat ttacaagctt
     1801 gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt gttcaacctg
     1861 gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt ttaaagacta
     1921 tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc taacttctag
     1981 gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag tttggttctt
     2041 taatttttaa acatgacaga aaattttttc ttcgttatac ttgtaataga atactatttt
     2101 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
     2161 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
     2221 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
     2281 tctagccaga caaaaagttc tagtgaaaca agtactcagt cagatatcaa agtgtctgca
     2341 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
     2401 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
     2461 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
     2521 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
     2581 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
     2641 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
     2701 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
     2761 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
     2821 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
     2881 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
     2941 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
     3001 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
     3061 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
     3121 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
     3181 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
     3241 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
     3301 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
     3361 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
     3421 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
     3481 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
     3541 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
     3601 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
     3661 tacagattgg taccgagctt cataaagaag ggccggaaat tttttctaga atcataaaag
     3721 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
     3781 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
     3841 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
     3901 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
     3961 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
     4021 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
     4081 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
     4141 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
     4201 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
     4261 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
     4321 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
     4381 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
     4441 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
     4501 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
     4561 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
     4621 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
     4681 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
     4741 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
     4801 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
     4861 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
     4921 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
     4981 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
     5041 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
     5101 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
     5161 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
     5221 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
     5281 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
     5341 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
     5401 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
     5461 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
     5521 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
     5581 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
     5641 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
     5701 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
     5761 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
     5821 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
     5881 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
     5941 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
     6001 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
     6061 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
     6121 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
     6181 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
     6241 tgtactttcc tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
     6301 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ctggtagggt
     6361 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
     6421 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tattttgggt
     6481 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
     6541 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
     6601 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
     6661 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
     6721 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
     6781 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
     6841 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatgcatcag tcaatctgaa
     6901 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
     6961 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
     7021 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
     7081 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
     7141 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
     7201 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
     7261 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
     7321 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
     7381 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
     7441 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
     7501 ttttccaaca gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
     7561 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
     7621 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
     7681 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
     7741 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
     7801 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
     7861 ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc
     7921 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
     7981 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
     8041 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
     8101 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
     8161 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
     8221 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
     8281 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
     8341 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
     8401 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
     8461 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
     8521 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
     8581 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
     8641 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
     8701 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
     8761 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
     8821 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
     8881 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcaa gcgtggatcc
     8941 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
     9001 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
     9061 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
     9121 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
     9181 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
     9241 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
     9301 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
     9361 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
     9421 tcgagaggtc gtgacatagc ttttgtcacg acctctcggc ttatcctacg agtattattc
     9481 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
     9541 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
     9601 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
     9661 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
     9721 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
     9781 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
     9841 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
     9901 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
     9961 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
    10021 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
    10081 aaatatttcc aaagctttta aaagtaactt tgcttttttg tatattatac cagcaagcca
    10141 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
    10201 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
    10261 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
    10321 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
    10381 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
    10441 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
    10501 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
    10561 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
    10621 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
    10681 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
    10741 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
    10801 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
    10861 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
    10921 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
    10981 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
    11041 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
    11101 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
    11161 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
    11221 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
    11281 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
    11341 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
    11401 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
    11461 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
    11521 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
    11581 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
    11641 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
    11701 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
    11761 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
    11821 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
    11881 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
    11941 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
    12001 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
    12061 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    12121 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    12181 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    12241 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    12301 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    12361 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    12421 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    12481 cagttggttc aaggaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    12541 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    12601 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    12661 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    12721 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    12781 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    12841 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    12901 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact agcttcccac caaattctaa
    12961 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    13021 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    13081 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    13141 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    13201 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    13261 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    13321 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    13381 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    13441 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    13501 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    13561 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    13621 aatgcaaatt atgagccaga cgaactagag gagacgaatg cggatcagga tacaggacaa
    13681 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    13741 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    13801 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    13861 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    13921 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    13981 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    14041 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    14101 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    14161 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    14221 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    14281 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    14341 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    14401 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    14461 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    14521 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    14581 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    14641 agaggaaaca aaaatacacg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    14701 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    14761 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    14821 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    14881 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    14941 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    15001 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    15061 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    15121 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    15181 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    15241 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    15301 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    15361 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    15421 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    15481 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    15541 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    15601 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    15661 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    15721 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    15781 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    15841 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    15901 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    15961 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    16021 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    16081 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    16141 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    16201 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    16261 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    16321 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    16381 gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg cgatgattta ctgagtattt
    16441 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    16501 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    16561 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    16621 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    16681 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    16741 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    16801 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    16861 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    16921 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    16981 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    17041 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    17101 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    17161 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    17221 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    17281 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    17341 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    17401 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    17461 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    17521 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
    17581 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
    17641 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
    17701 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
    17761 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
    17821 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
    17881 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
    17941 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
    18001 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
    18061 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
    18121 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
    18181 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
    18241 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
    18301 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
    18361 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
    18421 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
    18481 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
    18541 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
    18601 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
    18661 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
    18721 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
    18781 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
    18841 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
    18901 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
    18961 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
    19021 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
    19081 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
    19141 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
    19201 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
    19261 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
    19321 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
    19381 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
    19441 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
    19501 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
    19561 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
    19621 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
    19681 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
    19741 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
    19801 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
    19861 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
    19921 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
    19981 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
    20041 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
    20101 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
    20161 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
    20221 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
    20281 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
    20341 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
    20401 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
    20461 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
    20521 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
    20581 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
    20641 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
    20701 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
    20761 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
    20821 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
    20881 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
    20941 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
    21001 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
    21061 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
    21121 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
    21181 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
    21241 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
    21301 cttgcacata tcgatcgaat ccgagataat acctacgctt atctgattgt agatgatttg
    21361 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
    21421 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
    21481 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
    21541 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
    21601 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
    21661 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
    21721 cttttattca aaaaacatac caaaaatatt ccaacatcat cttggacttt ctacatgtta
    21781 ctaatgagcg tgtaaaagaa cgagatggtg tttttatttc tatcaatgaa gctctttttc
    21841 tttgcaatcc tcacttcaat cctctataat gtcgcatata gtttagtaaa tgagtaaact
    21901 actaatttac ttatttacta aaaataaaag gatgaggagt taaaataatg gaaataccaa
    21961 caaatttaaa acataaacca gtaatcgttg tagaagacta tgataaagtt gatggaagaa
    22021 atgcattaaa tacggatgca aaagggcttt cactgggcct tgctcagtgg aatgatcgtg
    22081 gaaaagtcga tatctcaggc aaggtatggc gacatactgg agaaaaatgg tcacgacagt
    22141 cggaagaatt accaatcaca cgtattttag atttagcgat cttagctgca caaggaagtt
    22201 tatacttcca agatgcttat cgttatgaaa aattttatga tccagaaaat ccagtcgtag
    22261 acatcattgg attacaaggg aaccgaatga cggtagaagt agataccaaa aatccaaaga
    22321 tcgatgaaga catcattttg tattacgata cactacaaaa agacggagaa ctgattgggg
    22381 aacgattccg tattttaaaa cgtcttttag atgagctagg atattaatag acagagatga
    22441 tcaaaagggg aagagaagca tggagaattt ctcactaaca gaaaaccgaa ccttttcacg
    22501 gctagaaaag caaatgatct ggaagaaacc gaaaagccat cggacaagtg cagaagaatg
    22561 gcggatcgcc tccgaaatca aagggaattg gcaagatcct gaaatgaaaa tcagtaacgt
    22621 acttttggaa ggagatgccg ggtcggggaa gacacaatta gccaaagcac tatctgctga
    22681 tctgcaactt ccctatacaa aagtgacctg ttttgcagat atggacaaat cagatgtttt
    22741 cggagcctta ttacctgtag tagattctaa taaggaacaa gaccaagaat tactagaagc
    22801 tatttaccaa acagatacac ttgaagctgt actgacactg atccagaacc attacggcat
    22861 tgatcctttt tctgctaaag aaaaattagc agagctagtt caaaggatcg aagctgatta
    22921 tccagatgca atccattaca gctattaccc ttctgaaatc gttcgagcga ttgaaaaagg
    22981 atatttatta gagattcaag agccgactgt catccgagat gcctctgttc ttgtggcact
    23041 taactcagcg ctcgaaccaa atgggatgtt gaatttaccg acaggaatca ttcgtcgcca
    23101 tccagattgc gtcattgtga tcactacaaa tcgaaattac caaggaaatc gcccattaaa
    23161 tgaatcttta agagatcgga tgcaacatgc ggaaaaaatg gatcttcctg ccttagaagt
    23221 catggcagaa cgtgcgatgg cgaaaactat gatccaaaaa caagaacttc tcttaaagat
    23281 ggcagagatt atccgtttgt tggatgaaac tgcaaaagct aacgccatca aaggtgtagc
    23341 aggaatgcgt tcttattttt attgggcaaa tacctataag caaggacaag atttactcca
    23401 gtcgatttat ccaaaagttt tgtataaatt atccactgat tcagatgaat tggcaattct
    23461 tgaacaagct ttgtctgaca gtggattgct actggaactt caggacatat tgagaaaaga
    23521 aaaatggggt catcctcaat ctgagcatac aaaaggaaga accattagcg ccgaagaagc
    23581 aaatgaacga aacatcactg aaaatgctga agaaattctg cgcttagatg aaaagaaatc
    23641 gttgtctgct gaagaaccaa atgagggaga agagaaacaa gaagaaacaa tagaaaaaaa
    23701 cgagattcct cctcaaatgc aaaaagaacg cagtagcgat atcgaaggaa aaaatcaaaa
    23761 agaacaacaa acaggagatg attctcaaga aatgtctgct tctgacatgg aagcacatga
    23821 taaggaaatc aagaagcaac tgaataaaga agcgcgacaa atcatgaagg aaacgattca
    23881 tgaaaaagaa ggtctcatcg ttcaccgacc agctttccaa ggaaaaacgc cggaagcacg
    23941 tgagctacac atgcaagtaa tgccaatagt agaatcactt agccggcaaa ttttagagtt
    24001 actggataat gaacaaacag agacgtacca aaaaggaaaa tacgaaggtc agcggttcaa
    24061 tgcaagcaaa gtagcatatg gtgatttgag aaattttgac aaaaagaacc cgccacatga
    24121 gcaaccttct ttagcggtag ctgtacggat cgatgaatct gggtcaatga tacgggatga
    24181 tcggatcgaa gcggcaaaaa aagcagcgat tgccattgcc gaattttcta agaaagtcga
    24241 tatcccattg ttgatttacg gagatacagc agaccgttcg tcaagagaaa agacttcttt
    24301 gttttcttat aaagaatttg aagacggatt tcaatggtta aatgaaaaat tagtcactat
    24361 gaaaccaagg caaaacaatc gtgatggtgc tgcacttcgt ttagcagcag aaaaattaaa
    24421 cagacaatcc gcaacaacca aactgttgct taatattagt gacggtcagc caaaagcgtt
    24481 gccggattat acaggcaaaa aagcaaaaga agatatccag tctgttttga aagaatatga
    24541 acgtcaagga atcctatttg tctccgcagc tattggccaa gacaaagaag aaatcaagtc
    24601 gatctacggt gaaagtcgtt ttgtagatat tactgacttg aatgaatttc ccaagcaaat
    24661 gattcaactg attgcccgct atctatgata aaataaagct caacgaacgc acttagcaga
    24721 ttcgttgagc ttttcgtttc tagttaccgg taacaaggat acttctaaaa tagaatcggt
    24781 tataatcaag agggaccgtt aaaggagtga aacgatatga tacaagaaca aaaatggtgg
    24841 aaagaagcag taggctatca aatctatcct gctagtttca aagatagcaa tggcgatggt
    24901 caaggagaca tcaatgggat ccgtgaaaaa cttaattatt tgaaagatct tggtatcggc
    24961 tttatctgga tcaatccgat ctatcaatct ccatttgtcg ataatggtta tgacgtaagt
    25021 gactatcaag atatagaaga gagattcgga acgatggaag attttgattt attgctaaaa
    25081 gaagcacatg aattagggat caaagtgatt atggatcttg tcatcaacca tagttcggat
    25141 cagcatcaat ggtttgaaga atcgaaaaaa tctaaagaaa gcccgtatcg cgattattac
    25201 atttgggtcg acggggtaga tggcaaagaa ccgaataatt ggacatcgat tttcggcggt
    25261 tctgcttggg agtattctca tgaaactgga cagtattatc tgcatgtttt tgcaaaagaa
    25321 caaccagact taaattggga aagcgagaaa ttgaaagaag aactattcaa tatgatccga
    25381 tggtggttgg ataaagggat agatggattt cgtttagatg cgatttctca tgtaaaaaaa
    25441 gatgaatatt cagtaaaagc cacagagaac cctttctcac cctttcaaaa tgtctcagga
    25501 atcgaagaac atttgaccga tttgaaacat gtttttgaag agtacgatat catgactgtc
    25561 ggagaagcaa gtggtgtcac tgcagaagaa ggaccacaat gggtagggaa agacggttat
    25621 ttcgatatga tattcgaatt tgatcatatc catatctggc agcaagaaaa agaagggcaa
    25681 ttagatgtcc ttaaattgaa acttgcatta agcgcttggc aaacttcatt ggatggtatc
    25741 ggatggaacg ctttatatat ggaaaaccat gatgttcctc gtgccgtttc agtgtttgga
    25801 gatactcgtc ccgatttttg ggctatgtct gcaaaagcaa tcgctatgat gtatttcttt
    25861 ttgcaaggta cgccatttat ttatcaagga caggaaatcg gaatgacgaa tatgcccttt
    25921 gaatcgatcg atcaagttga tgcagtagat tcgaaaagac tttataaaag gctgcttgcg
    25981 gaaggtaaga caagagaaga agcattggat atcattcgtg aaacaacgag agacaacagc
    26041 aggacaccca tgcaatggac ttcggaacaa tatgctggtt tttctacaca tgaaccttgg
    26101 ctgatcacta atccaaatac caaaacgatc aatgtagaac aacaagaata tgaaccagag
    26161 tcggtcctgc aattctataa gaacatgatc cgcattagac aaaccaataa aggtctgata
    26221 tacggttctt ataaagaata tcttcatgaa catccacaac tttatgtcta tgaaagatat
    26281 ttagaagatg aacaatatct tattatggta aacttaacag aatcacttgc tgactatgaa
    26341 cttccaaaag aagcagatca gtcctggaca ttactcttgt caaattcctc tagcggagaa
    26401 tttgaagcta aaggcatact agcaccttat gaagctcggt tatataaaac aaacaagtaa
    26461 aacttcctcg ttaaagttca ttagttggac tttgcgagga agtttttcag attgctctgt
    26521 caattactca tttcaataat tcgctctagg accaccgata tatttaggac gagagcgaag
    26581 ggctgtcacg tgagcttgtc caagggtttt caaacgaggt ctgacaggta cttggacatg
    26641 tttgatatgc ataccgattg atgtgtcacc gatatcgata ccggcttgtc caactacgtg
    26701 ttccacttcg acaggatcat tgaactggtc aaaagcagca acagaacaag cacctccagc
    26761 atgtagtgca ggaacaacag agacaatttc gaaatttttt gcttctgcca ctgttcgttc
    26821 tactactaat gctcggttca aatgttcaca accttggaca gctagcgcaa tttctttagg
    26881 atccaacaac tcttttaacg tacggatgat ccattgacca acttccgcac tggaattttt
    26941 tccaatatgt cctccaacga cttcgcttgt gctgcatcct aaaacaaaaa gatcaccttt
    27001 tttcaagtgt gcctcagcca atatgtcatt aactatttca gtcaattggt cttttaattc
    27061 tttttctgac aacttcattt tctaaaacct tctttttcaa ttaatattgc ttccgtgctt
    27121 gaatacgttg aagacttgca gttataggaa aggcaagtcc cattccaatc agattttgga
    27181 agatgtttcc tggtatagaa gccactccag ccggccaatc atataaaatc caaccggcga
    27241 taccatagcc gatgatcata actaagcttc ctaataaaag ccctaatccc cacatacgag
    27301 tagaagattt gctgaaagaa ccagcaacat atccttgtaa cccgtggatc actaatgaaa
    27361 aaactgccca ctgcggatat cctgaaatca aatcgatcaa tagaccactg gaagcaccaa
    27421 caactaatcc acccgttgga ccataaagaa aagcagcggt atagatcccg gcctcgcata
    27481 atgtcacaaa tccattcgta gccggtacag ggatcaaaat tgttaatgaa agggcaactg
    27541 tcaacgcaat cagcatagca tagatcgtta attgcttagt tttcatttct cattcctcct
    27601 ttttgaatac cgccagacca gacacttccg gtatgatcgt tcatcagcac gccattagaa
    27661 atacaatggg caacaaattt tttactttct gctactgctt tggtaaaatc tgcccctaac
    27721 acaagattcg ctgttattgc agaagaaaag gcacaaccgg ccccgtttac tgttgtagtg
    27781 tcgatttttt tatgagaaaa tacctcataa tgatcaattg aatagagaag atccacagct
    27841 tcatctccag ccatccttgt gccgcctttg atcacaatgt tttttgttcc tgaagcagtg
    27901 agttctttag cgagatggat catttctgtt aacgaagagg gaagagcttg accagaaagt
    27961 aaagcagctt ctgccaaatt aggcgtaatc atcgtaacaa gtggaaaaag ttcttcgatg
    28021 agctgcgaaa tatacaattg attgatcaca gcttcagttt ctttgaatgc caaaacaggg
    28081 tctaaaatga ttgggatatt ttgttttgtt tttatgaagt ctcgaacaac catgattgct
    28141 tcacttgaat tcaataaccc aattttgata ccatctaatg aagtcatttt atcaatcgaa
    28201 actaactgtc gtttaagcaa ttccaaagat atagattcca gcataaatcg ctcattaata
    28261 tctagtgccc ctatagaagt caaggcgctt agtccgaata gaccaaaatt ctcaaaagtt
    28321 tttaaatctg tttggattcc tccaccagca aatggatcag aaccgccaat tgtcaagatt
    28381 cttttcataa cattttcctc catgaataag agtataacga aattttcaaa aaagaaaaac
    28441 agccaattgg catattttta tctcatccaa tttaaaaaaa caaaaagaaa agaaagacca
    28501 aaatggatct ttctttcctt aaatagttag gacttttgtg attttaattc tttctcgatt
    28561 tgaagccgtt gcactttcat tgtagctgtt cgcgggattg catcaaaatc acggatgata
    28621 gggacgttca agcgaggaag ttcatggact tgctcccacc atcgattcca atccatttct
    28681 ttatcaggag ctaaagcaat aatcggttgt ggagaatgat ttttatccct tacaatcact
    28741 acttctgcca aaaaatctaa cgaatctaat aaaaagtctt caatagctaa tgtactattg
    28801 atcgtttcga tcaaatccac ttgccgatct ttcaaaaata ggtggccatg cttatccata
    28861 aaaccgtaat ctccgctatc ccaccaatcg ccataaacat tttcctggaa ccgtgcatct
    28921 tccttgtaat aagtcaaagc ccgtccttta gacagtaatt gtatatgacc atctgtgttt
    28981 tcaggtagga cattcccaac tgaatctgta atccgagcgc ttgtcatgtc ttccagtcca
    29041 acgcccatat ctcttgcatc acttgttttc aaactctcta gtgtatgagc cttaagaatc
    29101 attggtccgc attcactttg gccatatact tgaagaaata ccgcatcctg tgccatcgac
    29161 gttcgcaaaa atgtcttcat tgtctggttg tttatagcat caaaagtgga gtgatagtat
    29221 cggatacccg aaaaagcttc tggatgctcc ttagcagtaa aacgccactg aacaaagtta
    29281 ttcggatggg tctctagtgc aattggccgg tgagcttcca acatatgaac aacttttgaa
    29341 ctttgtgcgt ttgctaaagg catcatagga aatcccatgg acatcaaaga agagatacca
    29401 atattgaatc tggaatgcac tggagagatg tgaaaagcga ttaatttctt ttccgaaatt
    29461 tttgtgaaaa ctgttttttg ccatttcgtc cgccatccca tagaatgagc agaatgacaa
    29521 ataagtttag ggataccagt tgtaccagaa gtatgggtca tataagaaat ttcatttttg
    29581 tctaaaaatg attgagaaac agacaaagca ggttgttcca ttaaataaag aataggaa
//
LOCUS       DACIRY010000028        28196 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.028, whole genome shotgun sequence.
ACCESSION   DACIRY010000028 DACIRY010000000
VERSION     DACIRY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.028"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            71..940
                     /locus_tag="IYE22_001009"
     CDS             71..940
                     /locus_tag="IYE22_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ9506235.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            960..1307
                     /locus_tag="IYE22_001010"
     CDS             960..1307
                     /locus_tag="IYE22_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506236.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1320..2582
                     /locus_tag="IYE22_001011"
     CDS             1320..2582
                     /locus_tag="IYE22_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506237.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2662..4038
                     /locus_tag="IYE22_001012"
     CDS             2662..4038
                     /locus_tag="IYE22_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ9506238.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            4035..4664
                     /locus_tag="IYE22_001013"
     CDS             4035..4664
                     /locus_tag="IYE22_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506239.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            4676..5146
                     /locus_tag="IYE22_001014"
     CDS             4676..5146
                     /locus_tag="IYE22_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506240.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            5143..5625
                     /locus_tag="IYE22_001015"
     CDS             5143..5625
                     /locus_tag="IYE22_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506241.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            5622..6284
                     /locus_tag="IYE22_001016"
     CDS             5622..6284
                     /locus_tag="IYE22_001016"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_002351343.1,
                     RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ9506242.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            6271..6639
                     /locus_tag="IYE22_001017"
     CDS             6271..6639
                     /locus_tag="IYE22_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ9506243.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            6720..7166
                     /locus_tag="IYE22_001018"
     CDS             6720..7166
                     /locus_tag="IYE22_001018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506244.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRITEQAIEKQKDKEKKEIMSIYLSSGASFFHEID
                     SMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            7818..7871
                     /locus_tag="IYE22_001019"
                     /pseudo
     CDS             7818..7871
                     /locus_tag="IYE22_001019"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7875..8474
                     /locus_tag="IYE22_001020"
     CDS             7875..8474
                     /locus_tag="IYE22_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ9506245.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            8490..8966
                     /locus_tag="IYE22_001021"
     CDS             8490..8966
                     /locus_tag="IYE22_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506246.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            8983..9885
                     /locus_tag="IYE22_001022"
     CDS             8983..9885
                     /locus_tag="IYE22_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506247.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            9958..10149
                     /locus_tag="IYE22_001023"
     CDS             9958..10149
                     /locus_tag="IYE22_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506248.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            10313..10900
                     /locus_tag="IYE22_001024"
     CDS             10313..10900
                     /locus_tag="IYE22_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506249.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            10900..11799
                     /locus_tag="IYE22_001025"
     CDS             10900..11799
                     /locus_tag="IYE22_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ9506250.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            11799..12713
                     /locus_tag="IYE22_001026"
     CDS             11799..12713
                     /locus_tag="IYE22_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506251.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            12765..12980
                     /locus_tag="IYE22_001027"
     CDS             12765..12980
                     /locus_tag="IYE22_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506252.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            13165..13419
                     /locus_tag="IYE22_001028"
     CDS             13165..13419
                     /locus_tag="IYE22_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAQ9506253.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            13598..14641
                     /locus_tag="IYE22_001029"
     CDS             13598..14641
                     /locus_tag="IYE22_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ9506254.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            14814..15038
                     /locus_tag="IYE22_001030"
     CDS             14814..15038
                     /locus_tag="IYE22_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506255.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            15041..15298
                     /locus_tag="IYE22_001031"
     CDS             15041..15298
                     /locus_tag="IYE22_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506256.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            15400..15753
                     /locus_tag="IYE22_001032"
     CDS             15400..15753
                     /locus_tag="IYE22_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506257.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            15806..16600
                     /locus_tag="IYE22_001033"
     CDS             15806..16600
                     /locus_tag="IYE22_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506258.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            16629..17747
                     /locus_tag="IYE22_001034"
     CDS             16629..17747
                     /locus_tag="IYE22_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506259.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            17780..18100
                     /locus_tag="IYE22_001035"
     CDS             17780..18100
                     /locus_tag="IYE22_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506260.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            18355..19044
                     /locus_tag="IYE22_001036"
     CDS             18355..19044
                     /locus_tag="IYE22_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506261.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            19081..19248
                     /locus_tag="IYE22_001037"
     CDS             19081..19248
                     /locus_tag="IYE22_001037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506262.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            19268..20896
                     /locus_tag="IYE22_001038"
     CDS             19268..20896
                     /locus_tag="IYE22_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506263.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            20947..21318
                     /locus_tag="IYE22_001039"
     CDS             20947..21318
                     /locus_tag="IYE22_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506264.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            21403..21603
                     /locus_tag="IYE22_001040"
     CDS             21403..21603
                     /locus_tag="IYE22_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506265.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            21737..22963
                     /locus_tag="IYE22_001041"
     CDS             21737..22963
                     /locus_tag="IYE22_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506266.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            23560..23955
                     /locus_tag="IYE22_001042"
                     /pseudo
     CDS             23560..23955
                     /locus_tag="IYE22_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            24033..24545
                     /locus_tag="IYE22_001043"
     CDS             24033..24545
                     /locus_tag="IYE22_001043"
                     /inference="COORDINATES: protein motif:HMM:NF024954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9506267.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKNC"
     gene            24803..26023
                     /locus_tag="IYE22_001044"
                     /pseudo
     CDS             24803..26023
                     /locus_tag="IYE22_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352449.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            26016..26363
                     /locus_tag="IYE22_001045"
     CDS             26016..26363
                     /locus_tag="IYE22_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506268.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            26714..27535
                     /locus_tag="IYE22_001046"
     CDS             26714..27535
                     /locus_tag="IYE22_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ9506269.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            27555..28115
                     /locus_tag="IYE22_001047"
     CDS             27555..28115
                     /locus_tag="IYE22_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAQ9506270.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT        10744 a         3705 c         5717 g         8030 t
ORIGIN      
        1 tgacaaaatt tacaatatag tttattatat atttatctta ttatgataac acaataaaag
       61 gaatgatggt atgaaaaaga agaagtttag tcgatcgttg ttaggaattt tagttttagt
      121 tgcggttggt cttagtggca agggttactt tcagaataac agcactcagg aacatgattt
      181 tcagatcaaa agtagtgttc aggtaggtaa taaagcagac agtgaatcaa aagaattttt
      241 agggatacgt tatgatccaa acaaatatcc aaataatatc gtaaacatta atgatgggaa
      301 atatacactg acaaaagaag accatgaagc attatcagaa aaaggcagtc aaaccttttg
      361 ggtaaattat gcagatttag atcaacttgg aagagcagga gatgttgaag cgttgatcac
      421 ctatgatgct ataaattctc actcatctaa aaatgtggaa cgtccatctt tcaaaagttc
      481 tgtccatatt gcaggagaat acaaggatgg agtatttgat caagagagag atacttgggt
      541 cggcaaagat tcaaacaatg aaatcactca gctagaagga tataaaggtt atctctataa
      601 caaaagtcat tcattagcat ggtcgctagg aggagatatg gaggtacaca atttaacact
      661 tggaacgaga gcgcaaaatg ttgggaaaaa tgatcagaat ggagggatgg cttactcaga
      721 aacgattgta cgtgatgcaa tctataaaaa taagaatgaa aaagtattgt ataaagttga
      781 tccaatttat aaagggaatg aattggttcc tcgtggttct catgtcgaag ctttctcagt
      841 taacgataat ggaaaaacgg taaaccttaa tgtttatgta tttaacaatc aaaaaggaat
      901 caacatcaac tatcaaaatg gtacttgggt aagagattag aaattgagaa ggtgaagaat
      961 tggaaacaat aagcatcaat ggattagata aatcagttgc aaatcgaata aaaaaaggaa
     1021 atggctacta tcttctgaag aagggtaaat attcagttgc tagagagtta gaaacaatga
     1081 gtatcgtgta tgtagctaag aatcattttg acgtagaaca ggctattttt gatccagatt
     1141 ctgaaatagg gaaatgtgtt gaattatcag agaaaactgt tagtacaaaa ggggcgatga
     1201 tcattgagag agaaatgtta gacgaaatca gagtaatcat tcctaaagat acgcagatgt
     1261 gtgaggacag cataagtgac gatgaaccaa aatcagaaag aaaataagga gatattcaga
     1321 tgagcgaaaa atccattgaa ttaaaggaat ataagcgaag cgatggagaa aaatattatc
     1381 ctgtagccag tttttctaaa tcagtatatc tggttggacg tgcattggta gttggtgcaa
     1441 ttggcatata tattttgcat tgggtaacaa aggttgtaca actgcttgta gataccttag
     1501 taagtattct tgtctcgtta aatgatgtta acggaacaga tgatttagga ttaaaagaaa
     1561 tgtttaaaga gttactcaat gcgttttttc ccaattattt aagttattca tataatgtac
     1621 aaagcttttt tgcaaagcta cacttggtag acatctctat tttagtatta gaagcaggaa
     1681 cggtattctt tgttggatat ttggttactt tgatgttcag gcatcactat ggagaacaaa
     1741 gaccattttt tgatgatatg gaaagtagaa ggttgaaaag agaagctcta gaagctatgg
     1801 atgtgaagta tgatccaaac aagattatag gagatgaaac gaacaacaaa aaaagaaaac
     1861 gaaacaaaac gcaattagaa tccgatgtgc tatcaacgct taaaaatatc aaaatttctg
     1921 ttcatacaag acgtgatcta agtgtcaatg aagatattcg agaatatatc gtaaaaatcc
     1981 cgcaaccaaa agggaagaaa gttagagaaa cagttttacg acaaattcaa gatttagggt
     2041 cgatcctgac aagagcaaca agcggacgag ccaaatttgg tgaaaaagaa gatgaagttt
     2101 caggcgatta tttcattttc cgagcatcag taaatattga aagagaacgt aaggaaagag
     2161 taagtaccaa aaacaaagaa acggaaagag caacatcttc aatcgcagtt tcaaattcac
     2221 aattttcttt tccaatcaat ctctttgccg ataattccaa aaatatccaa aaccaaaccg
     2281 aaaaagctag aaaatatgcc gaaactcaaa agcaatcttt agaactttat ctatcttctg
     2341 ctgacgtacc tgcaagtcta acagatatgc acgttgggaa tacatctatt gaatatttgt
     2401 ttactttatc tcgtaccaga aaaattcaac taagctcaat gaaagaagaa attgaaactt
     2461 acttgcatat atcaggtgta atcgtgcgga tcaacgcagg gcgtattcaa attactctac
     2521 cattaggaga aaatgatcgt attccaattg atgtaccatc aatgatcaat agcgtgtttt
     2581 aaaagacatc acaaatagaa acagaaaata caaactacca gttcacaaac agaaaagtac
     2641 cgagaaaagt gaggaataga aatggctatg aaattattag agaaagtcaa aaagagagcc
     2701 gaaaaagctg aatctatttt gctagataaa gaacctaata taaagcttaa tggaaaattc
     2761 agtgtcaata tgttgaacac aaaggctaaa gacccgacag atgctatttt tggaattaag
     2821 cctgatggca caattgctca tttttgcatc agcgaaatgc cacacatgtt ggttgcagga
     2881 acgacaggat cagggaaatc agtattactg aatgaaattc ttgttacagc aatgtgtcac
     2941 tcaacgcctg atgaattaaa aataggaatt gtagacccaa aaagggttga atttggaaga
     3001 tacaaaaaat tgccgttcat gttggctgat ccaattacag atatggatga agcttatgat
     3061 ttttttgaat atcttgtgat cttgatgcat gaacgttaca aactgatgga aaaagcaggg
     3121 ttacaaaaca tcacgctata taacgaatat gccgaaaaaa atggattaga tagatttccc
     3181 tatgtgatct tattagttga tgaatattca cagttggttg gaacacataa agaagtagaa
     3241 ggtttgattg ttcaattagg acaaatggca agggcagcag gtattcatgt tatcttagca
     3301 acacaatctc ctcgatcaac agttgtcact ggtattatca aagccaattt tccgtcacgt
     3361 gtttgcctta tggtagcaag tgatttagaa agtaggattg tattagatga aggcggtgga
     3421 gaaagtctaa gtccaaaggg agatatgctt attaagcttg ttgatggaag tatggttaga
     3481 gcgcagggag cttacatcag taacaaagaa attgaaacaa tcttcaatca ccttagaaat
     3541 actatgccag aaccagaata tgttgattac cgagcaatcg tagctgaaac tcgtggtgag
     3601 ggcggtgaag acgaggattt tggtggaatt ggtaaagctc ctgtaagaaa atctgctaaa
     3661 agaaatttga acacaccttt agcaaatcca tcatctaaag agccatcaat tgaaaagaag
     3721 agtgcgtttg ctattaagct tgaaaaaaat aaagaagaaa aagctaatca gggagctaag
     3781 tcactagaaa aacttcagca agaaaaatta gaaaaactag aaaaagcatt atcatcatca
     3841 acttctgaaa ctcaatcttc aaagggtata gataatggac aatcaattac aactaaaaac
     3901 aagcctaaaa atagtaattc aaatctctcg gtaaatgttg ttatcacaac tgataaatct
     3961 gaaaaaaaac aagtgaacgg aactacttca cccatcatgc aaaaaatgat aaaggcaaaa
     4021 aaacaggtgc agaaatgatt atcattagtt cctttttgag atggctaaca ccatataaat
     4081 atacagatga aatagttgag aagcgagcga agaaaaaagt taaaaaaacg atgttaactc
     4141 ctaaagaaat gctagatcgt ggaatattgg gagtgaaggt tttagaagta aaccaaccgt
     4201 ttgaattgga taatgcgaga gataggttgc ttagagctga agttgatgtc gcatactttt
     4261 atcatgataa aagcaagcat caaatagcta tcaaattcaa tccaaaatcg gcatggcgat
     4321 accaagcaag agatgaccta aagcgtagga agctgaaacc gatgctctat cctgaaggaa
     4381 aagaggcatt tgatagaacc catattgttc ccattggatt tcatggtaca gagtcagata
     4441 accgcctatt ggtagggtgg aacagctcac aaaatagagt tcagatcaat aaatttgaga
     4501 agaaggtaag aaaatttaac gatcggaaaa taatactttg gtttgccaat attcagaagc
     4561 aaccagatgg gacagcaaag tgggaagcgg tcatatttga tgaagatggc aaaaaagtaa
     4621 tgtctgaagt atggcatgat aaagctaaat ttgtttggca gtagaggtga aaattgtgtt
     4681 aactaaccta aaactatata gagagggatt taacgaaaaa gtaagtatcg gtgaatctat
     4741 tgaatattta ggaaagaagt acattattat tcgtattctt gaaattaaaa aactttattt
     4801 tagtaaatat aataatttag cagttagagt tctcgtccaa aaggttggaa atccaagtga
     4861 ctatagaagt gattaccata actataaaac agaatcatct gtaattaagc gttataatca
     4921 atcaaaaaat gatgagagaa tattcaaagt aggagaaatt attatttcta agtatgggat
     4981 agcatacgaa atagtttcta ttaaaaaatt ttattatgaa tttgttgatt tggttgtaga
     5041 atattctgtt cgattgatcg ttccgtggag taaagaagaa attaatgagg cattaaaaca
     5101 tgaacgaaaa agtacattca aagttttaga aggtgaaaaa aagtgaaaac agaccttact
     5161 aggcaagctg agaagtgctt atggcatgaa acaaatcgca tgggtgtatt tggttgtttc
     5221 gaagtcacta tcggttggga tggcagtgaa agagtagatt atataactta ttcagtagat
     5281 aacacagttc gatgttatga gatcaaagtt actttagctg atttaaaaag caaggcgaaa
     5341 cagactttta tcggtgatta taactattta gttgttacta gtgatttgtg ggagaaaatg
     5401 caacaagacg ataaacttat gcggaaatat tactatcaag gcgtatacgt gttcaatgaa
     5461 aaatatccta gcataataag tgttaagaaa gcaaggaaac aaaaagtatc gctaggtaca
     5521 agatcaactg tattagaaag catggttcgt tctatgaatc gtgaagttaa gaaactctat
     5581 aaagtaaaac cattttggga gatagcagag gtggaaaaag gatgattcaa acatttgcaa
     5641 aaatgagtag aagtgcttgg attgaaaagc tggaaaatga tttgagtgtt atgcgattag
     5701 ctccaaagaa attcaaacga gagattgcag aaaaagaagc aattttgtcg gtattgaatg
     5761 cggtagaact ggacgaaccg aaaaagtcag ttgatgtacc tgaaaatgta gcagagttac
     5821 tagattatta tcgtaattcg tcagatgtcg atttgttagc tctgatatta acatttcacg
     5881 attggtatta ctgtcaaaat gaagatactc agcatgataa acatgataaa gcagttgact
     5941 ggttaataga gaatccagaa aagttcatgc gtgcttggct agatggctat gatagcaaga
     6001 aaaagccact ggaaagtaat gaggaaacgt atttaacggc agaacaatat gactatgcta
     6061 gaaaaacagg agattgggaa tctggtaaga ctttcaatca cagaaaagat caaccaaaaa
     6121 ttagtttttc agatatagcc gcaattatcg aaggtatcta ctatggacaa gccgatgaag
     6181 agatcttttc tatacaagat ttattagaat ttttgaacaa aaataaggat agctttgtag
     6241 aactaactga ttatgaggag gatacagcaa atggaaaatt atgatccaaa tatgcgatgg
     6301 ggtacacata cgcttaaagt ttcttttcaa cgttgggatt acaagggatt tgtgactttt
     6361 agaaaagctg gaaattgtaa aggattagat gtcttagcgt tggatgaaga ctatctatat
     6421 gaccatccat tgacggataa tccaatcgga tttggattgc ttcctgaaga tgatgagggc
     6481 aatgaatggt tcaaaatgat tcttacaaac gataaaggtg atcaattatt tgtagaagat
     6541 acttggtcat atttgagtga atatattgtt agcattaaaa tcattgattt tgtagcagat
     6601 aaagaaaaag agattggaga aggtaaatct aattactagc taatgaatag caactttgga
     6661 ggataagtca aaagaataaa aaaagaatac tgtgtgtcat tgcagggatg tatctttgca
     6721 tgatagtatc tgcggttacc atttattggt taggaaatca gcaaaacagt ttaattgttg
     6781 atctggggga gttaaaggtt ggattacgaa tgactagatg gcttttatta gctcaaacag
     6841 ttggtttagc catattgact tggatatgtg tggaaaaaat aacatctaat aataatcaag
     6901 agattgttga atcaacaaac ctaagaataa ctgaacaagc aattgaaaag caaaaagaca
     6961 aagaaaaaaa ggaaataatg tcaatttatt tatctagtgg agcatcattt tttcatgaaa
     7021 tagattcgat gaaactgagt agtcaagaaa tcattgatag atggaaaaac atgcctctag
     7081 gtggtgagtt aatcgttgaa gatagtgatg gttggtcagc ttataagaag gacgagattg
     7141 tagctatcac gattttgttt gaatagtaat atattaccac tagataatct gaatatgttg
     7201 atctgctagt gacgtaccaa atgcgtttaa tagaaccttg gtcacaggca gaaattgata
     7261 aagcagtaaa attgaaccga ttatcaaaat ttaacgtatt aggaaatgcc ttttaaaaat
     7321 cgcaatacac aaacagaaaa ataccaaaaa attagcaatg aacgtaggta agaacatgac
     7381 aataaataat aataacccta acaaatataa tgctcttgtc caatgctata tcgaaaacaa
     7441 aattacaatg caagaagtcg ctgatgaatt aggcaaatca aaaagcctag tatcacacga
     7501 ctttaagcac aatgtttttc cgaacccgat tgagctgagg aaaaaatttc aaaagagagt
     7561 ttatcaagaa atagggaagt ggattgatga agggaatgca attgaccctt taattgaaaa
     7621 atatcctttt ttaaaaagca aaaataaaat tcaaaggtta attcgtcaag gcgatattca
     7681 agctaaagaa cctgttttga caaccaatga tttggctaaa ataaatcttc ataaacagat
     7741 tattgaagac tttgaaaaaa atctttcaac aactgaaatt ttaaaaaaat acaatattcc
     7801 gagaggaaag ctaacatcga ctctttttag agaatatgga gaatcggcag ttactaaagc
     7861 aaaggaagta gcgaatgaga gaaaaaaaca aaaatagtag tcgatactta gtagcgactg
     7921 tagtttttgt ttgtgtattg attttcctgt ttgatttttt acagattgga aaaagtataa
     7981 ttggaaatac attgagtgca aaggaaatgg ttgctttagg tggtttgtat gaaggacatt
     8041 tgttcccgaa tatattcatt tctatcgttt ctcatattaa cttgtaccat ttaatttcta
     8101 atttattgtt cttagtagtt cttgaagaag cactgaatga tcagtatgag tggttaacat
     8161 tattgatcgt tttaatcata tcaggatttc taggaagctt agttcctttt ctgctacaaa
     8221 gcaacatcgt aagtatgggg cttagtggag gtatttttgg atggattgct ttatttgcga
     8281 ttgcatacat ccattttgat agcaaggaac atttattact aagtgttttt gtattattga
     8341 taatcggcat tttattggta tctacttttg aatcaggcga ggcaaatgtc ttgtcacatg
     8401 taatcggatt tgtctgtgga atgatcttgg gatttattct aaagaacgtt tcattgatca
     8461 aacaaaagaa ttaaaaggga gtgctaaaaa tgccaaaaag aaaagaaaag aaaattccag
     8521 tgtttttgtc tgtctcagaa atgttatcac ttcaaatact gttaagaaaa aagattgaaa
     8581 caatagacga agaagttaga aataaaaggg ctatgtacga acaacgtgaa gcgctaagag
     8641 aacgaatcca ctattttgat gatatacaca ctaaaatcta tgaagcacgt acaaatcacg
     8701 aagacgaaag taaattagga ttagctcaac aacggatact tgaatggctt aatacgaata
     8761 ctgatcaaga accgttaaga gtgattagta atctttttga atttggaacg aagcattggt
     8821 ttaatgctgt agagaaggat gctgacttac aagaagcgtt tgatacgcta agtaaggata
     8881 gcaaatccat gcgagattta ttgattactt atctaaatga gcatgagttt gaacaaacga
     8941 aaaccaaaaa gaaaaataaa gaatagaaag ttgtgatgaa tcatgctatt aaccctgtta
     9001 ggttatttat gtttaattct tgcttttttt ggggcaatcg ctataattat tggtcaattt
     9061 caaacaaata atccgattat gcgtaacata ggcattggaa ttgtgattgt aacctttatt
     9121 gtgtttaatg ccactaagat ttttggaagt aacgataaac aatttggtat atcgggagtt
     9181 aataatagtt atgctccaga ttctttggga agggtagtaa tagatggaac atttgaaaat
     9241 gtaacgagtt ataaaaatgt ttcgctgaca gtgaaggaag atggcaagaa agcatcagac
     9301 gacgttctcg ctactattaa gccgtctcaa tcacaatttg aagtgtttta tacagttaaa
     9361 gagaatagcc ctgtacacaa gctgacattg gtttttaacg ttgatggaga agaatactat
     9421 aaagatgtaa aaataaataa aacatcaacg aaaaagatta tcgaatcagc gaaaaaagaa
     9481 gtagctacag aagaaagtac aactgaatca agttctactg tcgaagcgga actaccagat
     9541 aaaattgatt ttgatcctga aaaagtcaaa tcgtttgaat ctgtttctgg ttctatggct
     9601 gatcggttta acactgaagt cttcaaagct atcaaaactg ttaaattgga tactgaaaag
     9661 caaatgatta atgttacctt attcaattca gttgaatcta tggatcagga taaaaaagtt
     9721 acggttgcaa gagaactagg aaaaagagta attggtttgt atcgtggtac tgctaaacca
     9781 gattcagacg gtgcagattt gccagttcat gtagtttatg aagacggtaa agtttttgga
     9841 gattctacag caaacgaagc attaacgatt aaattggcta aatagccaga aagttataat
     9901 aaacaaatgg tgtcacacca ataatatata ggtgtgacac cttggagatg atagctgatg
     9961 gtagtaaata aagaaaaagt tagaaagtat gtgactttaa aaatagcttc tgttaaatat
    10021 ttagaaaagc gagctaaaga agaagaaaga gcaagaggta agaaagtaac agtttcagag
    10081 ttgttagaag aattgatcga gaatgatcaa acaataagac gtgcatttac taataggtct
    10141 atcagataac aatataatta ataaaaaaga aagagtgatt tggtcttaga ctaaatcact
    10201 ctttttatat aaagtttctt tacactcatt ccgaataatg ttaaaattct ttatatgata
    10261 tatagttcct tgtcagagga agagataacc agcaatattg gagggataat aagtggaaaa
    10321 caataatgat attagacatt ttagcattgg ggaaagagtt acaacagtaa gtgaattatc
    10381 aaaggattat agagataaag agggaacgat tatttctctt aataagaatc gaagaggcgt
    10441 ttggcaatat gttgttgatt taggtgatgg cgttacagtt gagtgtaaag atagtcaact
    10501 gatcagaaat aatggcagtc attccattga aaaagcaatt cttgtatacc caaaagtatc
    10561 aagtaagatc atgaatgact ggttgcttgg aaaatttggt gagtacaata aaaaagaaat
    10621 cgcagaagtg atcggcaaat cttatccaac agtattaggg tatctaaaaa agataacgag
    10681 taaagaagca aaaattgagg atgttttaac agtaagagag cttcaatcat taatcgagta
    10741 tcggaataaa caaataacaa atcctgagta ccttagaaaa gaagatggtc tattgaaaca
    10801 attggaacaa gtaaataaaa tccggaagat gcaaaaggta gctcctttaa gccttgatac
    10861 ctttaaaaaa atctatttga aagagatcaa aaaagactaa tgtggactgt aactttaccg
    10921 ttccaacctg tatctgcacc aagaccaagt gcaagaagga atgctgctga tgaaactaat
    10981 gacgtagcag agaaaaggat ttcaacctac tatgataaaa agtatcttga ttatttgaaa
    11041 aaaataaaga gctttatcga agatcaagat ttattagatg atgaatttta ctcgattgta
    11101 aatgatccta tgggcgcaat tatggaagtg gacttttact accagatacc aaaaagctat
    11161 aaaaaagtct ataacattat gaagacaaca gcgccagatt tagataattt agtaaaaagt
    11221 gctatggatg gtatttttaa catctcagct atacgagata gccatatcac aggcttaatt
    11281 gcttttaaat ataatgtggc gaacgaagga aaaacagttg tgaggatcaa acgatattca
    11341 gaatttgagt gggatacttc tgaaggattg aacggagata tatgggaagc tacatttgat
    11401 tttaaaccag tagcaacgcc aagaccaaag tctaaattta gaggaaatgg gattatcact
    11461 tactatccaa aagagtacaa cgactatttg gaaaatatca agaatacgct taaagagaaa
    11521 gatttggtaa atgatcaact gaaaaaggta atgaatgctc ctatgggggt tatagctcag
    11581 atagattatt tttaccaagt gagaaaagat aagaaaaagc ttgattcact gatgcgtact
    11641 tcacagccgg atattgataa tttagtcaaa ggaacattgg acggaatttt taacaaggaa
    11701 ataggcatca aagatagcag ggttgtaggg ttaattgctt ttaaatacaa tacttttgaa
    11761 aagtcgaaaa caaacgtgag actacaggag atgggctgat gaataataat gaaaaaattc
    11821 ttcatgtact tgatagcttt gaaataattc aagaagaact aaaaaaatat cgggatgttt
    11881 tggaacaaag atatgatttt gtcaatcagc aaaagagtaa tcacatggat ttcattctca
    11941 atatgaatga cttgaagaag aaattagttg aacgaaaaga acaagaaaag ttgatcaaag
    12001 cttactttga actcggtgaa aaagaagtta aaaatgcaat ggaactgaat gaagagcgca
    12061 gggttttaga tcagctgtta gaacaattat tggtgatgtt tcaaaaagga cgaatagatg
    12121 aggatttaat tgaagaagga ttgagaaaat atccttcaaa ctcaggtatc ggaatagtgc
    12181 tgaaagcgat tgatgaagaa gaaattgaag ctttcatacc gccagaggat tttgaatcgg
    12241 caatggaata tataaaatat tatagccaag ggatcactgc ttttagagag tttgatcctg
    12301 aagatgttat tcatgacttg aacaacctaa aagaatggtg tgaaagttat ggagtcgatg
    12361 attccggatt ggactacttg atcagtatta tggaaatcga agaagagatg ccggataagc
    12421 ctgatccaac agatatttta gaattgatcc atgaagctag aaatccaatt gcctatattt
    12481 ctagaggata tactgtattg gaatactata agccttatat atcagcgatg aaccatttaa
    12541 gacgtgtatt aagggaaaaa cgtgaatacc gctcagtgtt gaatgcaacg aatagattag
    12601 aaaaagctgt ttcagagcta gatgcttatt atcgtgaaca ttacctgcaa gcaggtggta
    12661 tgccaagaaa tacgaaagca aatatcagta ggtatataaa aaaagctgag taggcggagt
    12721 tagcccataa aatggcaaaa ggtaacctga aaggggcatt ttgaatgaaa agggatgttt
    12781 ttgaattgta cgtattagaa cgtctagaag acggcatgat ggcacaagag ataattgatg
    12841 aaattgatga tatgatcagc agatttggtc aattggagtt gaatgatgga aacaaaaatt
    12901 cggtagtgct aaaacaattt tggttgaaca atttttcgta taaggatttt atcaaagaaa
    12961 aggcaaatac attgtactga ctaaattcac gaggagcagt gatgagagct tattgctaag
    13021 agcagttagg ctcttttttg tttgtgtaaa attatttgtt ttataataca catttataca
    13081 caaaaaatat tgacagatag tacacaaatg tggtaggata tatttgtaag caagttagat
    13141 taacaagtta aaggaagagc agttatgaaa aaagaagaac taatagatat atttcaaatt
    13201 gttgaacgag caaataatat gggaattatg ttttttgatc gtatttcttt aaagatggat
    13261 ttaagcgttg cccaccaaga atttaatctg cgattaaaag ccttgttgaa ttctgatgat
    13321 gttaatttcg ctcatgatgt tgtaggtata cagaaccata tagatagaga aaacaaacgt
    13381 atgggtgatg aattcttacc aagatattca agtttatgat ttggcgaaag attcaagagt
    13441 atgtgtcatt attgataggt actcttgaat tggaaaggag gaatgaattt aagacaacaa
    13501 cgattatgat cttaaaacaa tcgtttatgt aattggcaat cttacgaata attttcatgc
    13561 aataatgaca ataaaaaatg aaaaggggta aaatcaaatg aaaaaatttt tagtaggaat
    13621 tctattcaca atggggctag tattattatc accttcagtt tcagcagaaa gctctatttc
    13681 catgtatcgt atgtataatc ccaatagcgg agagcatcta tacactagaa gcgttggaga
    13741 aagagacaat ctgaaacgtt atggatggca ttacgaaggt gtcgcatggc aagctccaac
    13801 aagtggtact cctgtttatc gtttgtataa tcggtataac ggtgaacatt tttacacagt
    13861 tgatgccgga gaaagagata agatttcaca cacaggatgg acgtacgaag gtgttgcgtt
    13921 ctattcctac aacggagcaa atagagttcc tgtaacacgt ttatataaca aatacgtgaa
    13981 ttggcatcac tacacgttag atcaaaatga aaagaacgta attagtaaac aaggttggaa
    14041 gattgaaggt acaggctggt atgcaatgcc aaacagcgta ccaaatccta cacctgctcc
    14101 aagcactgaa aaagtgttga acgttcctgt tgtatggcaa ggaaatacaa tgttatgcga
    14161 aggagcttct ttattagaag gtttacacta caaaggtgta gccacaaatc aaagcttaac
    14221 cagttttgta aactatatgc caaaagctaa tgacaataat ccttatcatg gttactcagg
    14281 agagtggcgt cataacgtta atggaacgta tcaaggcatg atggctgacc cagtagttaa
    14341 atgggcaaaa aacgttggcg gtaatgccgt caatatcact ggttcaggaa cgaatggaat
    14401 caagaatgaa attcgcaatg gtaatccggt tgtaacttgg attacttata actatgctac
    14461 gccagagttc aaacaaatgc cttggggtcg tgcagtatgg aatggacatg tagttctcgt
    14521 ggatggcttc agaaatggtg cataccatgt tgttgaccct gtatttggcg caaaatggat
    14581 caatgcaagc acgtatgaga gagcatttaa tgtgactgga atggcggttg ctgtacgtta
    14641 attgatagaa ttatcaacta aatgaaataa gagcttaatt actgaaatgg taaataggct
    14701 cttatttttt tagaggaaat ttttgtaata cgaatctata acaaaaaata ttgaatgtag
    14761 tacacaaaag tggtatatta aaatcgtaga gattatgcga agaggtgatt ttgatgaacc
    14821 aaagaagaag aaaaaggtta gaagaaataa gcgagcgact ttccgagcta aaggatgagt
    14881 tagaagtaat tcaagaagaa gagtacaaag aaaatatacc taaaaatcta cagggatcag
    14941 aaagatatga aaaagcagaa gaatccgttg atcaacttga agaagctgtt gatggtttat
    15001 ccgacatatt agaattgatt gaagatatag ctagctagag atgattaatg aagatttcag
    15061 gagtgagaat aaaatgattg atatttcact gatagagaag ttacttttct caaataagta
    15121 caccgcaaaa gagctggcta caagatggca aatatccgaa tcagctatta aagcctatcg
    15181 tcaaaataag ggaagcgtag gatggcgtga ttggaaaggg atcagcattg gaaaagcaga
    15241 agacataatg aaacgtgaag gacttcgatc ctataccact tctgaaaaag acaaataact
    15301 taatgtttga aaagataaaa tatctgtctt aaagctatgt caaattcaaa tggtgaatgt
    15361 ttgctaaata taatgcaaaa aaaggaaggg aaatattaaa tgataagcga aaatttgtct
    15421 tatgagaata ataaacatct agttaaagaa aataagaaag tagaagatta taagaacatg
    15481 tatatagaaa tgaaaaaatc accactgtgt attcaatata aaaatgataa ttggaagcaa
    15541 gtagtcgaat ttgtaaaacc tcatagtata cgaagagtac gaagggggaa gaaaaaaagt
    15601 gaacttgtaa atagcggaag agcggtaatt agagttataa ccaaagatta tccagttgaa
    15661 tatggagatt atattttaag gttaagttat catgcttttg ttgtgttgaa agaagagcat
    15721 ttcaacgagt ttttcaattt aaaaagtaag taatcatatt tttttgtaga cgtgtataaa
    15781 gttaactaaa ttgcgaggtg atgacatggt agcaaaaaag aatactctaa aagaagcttc
    15841 tgaaaaatat caagatatga aaagacgagg attaataccg aaagacaaaa aaggtatatg
    15901 gagagaattt gttaaggaag tcagtagtga tcgaagtggc ggtgactttg ttggacgata
    15961 tacagcaaaa ggctatagca aacgattaag gatcactgtt gaatatggaa cgaatgaatc
    16021 agctacagag ctgaagcaaa ggtacttaga tacacgaagg gaagcttcaa aaaaatataa
    16081 tgatttcaag agcagaggtc tgattcctaa aaagaaaaag ggaatttggc gtgagttttt
    16141 caaggaagaa gatagcaatc ggaataaggg agacttcatt ggacgataca cagcaaaagg
    16201 ctacactaag aatttaggga tcaaggttga atacggaact ggtgaaagtt catgggaatt
    16261 gagaaatcgc tatttaaata cattgttaga gaaatataaa gcagaattat tggagttgtt
    16321 cgatgttaat gattttatcg agatggctga aaagtatggt catgaagaag gttttatgat
    16381 caatgacatt atggttattg ataattttac ggatttatta gcacttgcga aaaaagcaag
    16441 ggatttaaga gaaacgcaga tgtatctatt cagaaataaa aacggaagaa accggagaac
    16501 tgaatcgagt gagattgaag attactacga tgcctttaca cacgatctgg gagcctttgt
    16561 agacgagata agtggtgtaa tgaacaaata tagggtctag aataaaaaag ccggagatga
    16621 gcagataaat gggaaacaat agcatataca cacctgaaat gcagaataaa attaaaagac
    16681 ttgtcggatt agggtggatg acaagaggaa aattagattc ttttagtacg atcaaatatc
    16741 ttttgatgga catttaccgt cagaacaagc aaacaggaat acattttttg ggctatgata
    16801 agccggaaga tgcacctttc tttgttccaa gttcagccta tagagccagt acctccggta
    16861 gcatgtataa cttggatttg tttgaggaag gagcaaagat gatcgaatat gatttcaacg
    16921 aagcctattc gaggatcatg agaaattatc ctttgccgtc aaacacttat ttgaaagcaa
    16981 aatataatac agatcaagct attttgagag cgataaatag accaaaagta agaccgtatg
    17041 aaaatttaag aacgtatatg ttctttttgg ttaattttga aggaacagcc aaagaaggaa
    17101 catatgctaa tccaaaatca cagtcgatgt ttgcggatta tggcgaaaat cccaaaacag
    17161 ctaatgagcc tatttggtta tcggagattg agctaacact tatccttgat tattatgata
    17221 ttaaagagat ggaagtccta gatagccaca catttttgac gaaaaaagga ttactcaaag
    17281 aatattttga aagaatcgag ccattaaaaa ctgatcctga tacgttgaag ctttataaaa
    17341 atcttagaac gaaagtattt ggtgctatcg gacaaaaatc attaagagaa ggagaaacaa
    17401 gcaccttcga taaatacagt atgtataatc gagcgtattc ctccatggta gcaggcgtat
    17461 ttagagatcg tatggttaga tatgaacaga aatacgttca tagcgcatat gggctattaa
    17521 aaattcgtac tgatggattg tatttcagat ccgaagtacc agagtttgaa gagctgtata
    17581 aacttggagt agttaagaaa aaagttcata caatatccaa agaagaagtc gaaaaagctt
    17641 tagaatttaa aaacaaagga tttgaaaata agggctcctt tgggaaatct aaagatgaag
    17701 ttaagggtgt tgtgatagac gatgatttcg agtttgattt tgactaaata ctctttggaa
    17761 aagatggtag gaggtaaata tgaagaagaa aattgtggaa ggaaatgtag gtgttattat
    17821 tctaaaagat tcaactgact atgctaaatc aatggcacta aaagacgaaa aagttgcacg
    17881 catggataaa gatttagttg aatttctgat ggatgataca gaaagattaa aaggggatca
    17941 gtttttgagt ttaatgaaag acaaaggctt aaacacatat ggtttatttg attataagaa
    18001 attagctata gagtgggtaa aagaaggaga aatatttttt attgaagaat gggaaggtac
    18061 tgagaactta gttcttcatc agtatctttt gaaagcttaa tctaagtaaa acagttctga
    18121 ctagttatgc ttgtcagaat ttcaaaggaa gttttccata accatcatca ttcaaaaata
    18181 aaggatttga aaataagggt tcctttggga aatctaaaga tgaagttaag ggtgttgtga
    18241 tagacgatga tttcaagttt gattttgact agattttttt gaaaggttta agagtaaaca
    18301 aggtaagaac taagatcatg aagattgaga gtaaagaaga tagaggacga atgtatgaga
    18361 acacactata tttatttaga agataaaatt gtagatgaaa ttccggcaaa aagtcgagaa
    18421 caagctagaa aattatttat tgaaaaatac aaagagatca atggagatag tgaagttcca
    18481 aaagtggtcg taacagttag aaaaaggcaa acaaaaaaga gaagtacgtt tcatgtcagc
    18541 cctgatatat ataatcttat agagcgacat agaagtgaag ataatatttc tatctctcaa
    18601 tatattgcta cagttattga tgaattgatt tcaggaaagt atgactatgc ttttgatttt
    18661 gagggtgaaa aggattacaa aagtgttgct gtctttcaat ctgattttga gaagctaaaa
    18721 aactatgctg gcaggagaaa tgttaatttt gcaagactac tagaagagtt ctactccgaa
    18781 gagttgggag taggaaatga atacaagaga gaaacttatg cgaaattgtc tcataagtta
    18841 tcccaagcgc ttaaagaagt gacagaagtt aaagcggaac tagatgctaa acttgaagaa
    18901 gtgaaaaaat atcaaacaga gaggaagaac aacatgaaga atcaagtgga atttgacgaa
    18961 cagaaagaat ttgaaaaagc aagaaaacgc tataacaaaa ttatgaatga acttagctat
    19021 gaacacttaa cagttcatga ataattgagt gagggtacag aaaaatatac gatcgcagat
    19081 atggttaatg aagtggaaac tcttttagct tctttctttg aaaatggtca cgtaaacgat
    19141 gaatggcgaa aagaagaacc taaaagggtt aacgcttata ttgctcgatt aaaaaggttt
    19201 attgataaat ataaggagaa tagtgacaaa ttttctaaag ataaataagg agagagtgtg
    19261 attacatttg ccagaaatcg aaggaatatt ttcgccaaaa ataggtaaaa aacagttaga
    19321 tttcagcagt tttccgagcg taaatagaga ttataatcta ggtgatgagg tacagatact
    19381 aaatattgat ttcaaagaac tagacttcgc tgttgctgtt tccaacaaac ccaaggcatt
    19441 ggaacgagtg aaaaaaatat accaagagaa tgatcaaaat atcaaaaata tgatcaaaaa
    19501 gtatagacga gataattcaa aagttgaaga cgttttattc aacggtacgg caaaacatga
    19561 tttgaatgtt tatatggcat taggagtggc tttgtactgt aattcaatta atcagacaga
    19621 tattttggtt aatatcgtga ggaatagcta tcaaaaaata ttaaaaataa gtagcgaaac
    19681 aaacccttat tatgcaatga aatatcttgg ggataattta ttaaatgata aaaaattgat
    19741 gcacaacact gaagagctgt tatctaattt ggctgcgatt aactttctat tcaaaacagg
    19801 ttctgaagat tattttatag ataacgtact aaaaggtgct tacggaacag cattttatcc
    19861 gctttacaat atgaaacagc ataaagccga acaaaaggat aacaaacttg aaattaaaaa
    19921 gatgaaagaa ctattaggta taggatttgt aaaacccaat tcaaaagtca ctattgcttc
    19981 gttagcaact actaacaatg atgggaaaat agtaaatggg tttgacgatt cagatataga
    20041 tatattgtca aaatcatatt tttctttgta tggagatttt gcagatttga tgggtattga
    20101 tagttactta gtcaataatt ttgagatcaa gggtagcgag ctgaatgata ttgtgtattt
    20161 cgttttaatc tctctatatg caattgatca gaataccgag taccgacaaa ttgactattt
    20221 gctagttttt tatagtttgt tatatcagtt cgttttaact cggttgttta aaaatgctaa
    20281 ggaagaattg atcctgaatt ctgattatga aatagcaaaa caaattgcaa gtattagaaa
    20341 agaaaacaaa tctgaagtaa tcaaattaag aaaagaatta agtattgcga gtaaagaaaa
    20401 cggtcggtta gaatctttag tcaaatcggc aaaaaaagaa gaggaacttt tgaagcaaga
    20461 gattaaagaa ttaaaaggaa tcagaaaaga acttgaacag ctgaggtttg aaaataaagt
    20521 attaaagcaa ttgcaggagc aagaacatag acaatcatta aatggtaaaa ctgaagagcc
    20581 ttcactgtct gaaatgatag cttatctaaa acataaaaac attcttttag tgggaggaca
    20641 tgtaaatttt caaaataaat taactgaact attaccaaat attaaaatgt ttgatgtaag
    20701 cgaaaataat ggaaagatac ttaaaaattt gaatacgtgt gatgcgattt ttctatatac
    20761 agattacttg aatcatggtc tatattatcg tgttatgagt gaaatttcta ataaagataa
    20821 gatattttat ttggataatc atgtgaatat gaacttaaca attaaacgta tttacgatct
    20881 tttatcactt atataaagag agaagtgata aataggacac aaaatttgga aagtaggtaa
    20941 gtcactatga gagaactaac tttatcttct aaggaattag aaaagaaaac tgctttatta
    21001 aacgaaatat ttagtgctga ttgtataatc caaagtttaa aagaaagttc attttcaaaa
    21061 ctaacagatc aggaaaaaat ttcttatatg ctaagtttag ataatctagg aactaaatat
    21121 gatgaatcag caagtagcga taagcttttg attgaattag tatcaagtct tagtagtttt
    21181 ggaaaaatga aaagatttaa tgtgatatta gaaatttttg cttggttaat cccttcattt
    21241 atttggtttt tttatatttt catagttgaa tctgagttaa tagccaaggt ctctcttgga
    21301 ctgttaattc cgatataaat aggttcagca atttacttta ataacttgta tacacctatt
    21361 caaataaaaa agcagttatt acatgaagga ttaagtggta atatgttaaa aagaattgca
    21421 tgggataaag ataataaaac agatattgag cttaaagaat ataaatacaa ttatttctcg
    21481 agatgtatag atagaaagtt actgtttaaa aaagaaggat tagaatttag ttttgatgaa
    21541 gatgaatttg aaaaattagt gatggactgg cgtaagaatt tactggatac ggtacaattc
    21601 taaaagcaat tgaggtcgaa attccgatac ttgaatcatg atctatatta tcgtgttatg
    21661 agtgaaattt ctaataaaga taagatattt catttggaca gtcatgtgaa tatgagctta
    21721 acaattaaac gtatttatga tcttttatat gtttataaac attaatgagg tgctaaaatt
    21781 gacaacaata actatgacaa aaaaaataga agtatatgtt gatggatcac aagaagaaag
    21841 ggattccttt tttgattttt tttggaaaaa tagtagagaa tatgtaaaag cgttgaatct
    21901 ggtatataaa cagctaatgt ttgaagagtt gataaaagat gcaattaaag aaatggatca
    21961 aggttatgct gagagagaag aaagtattca aaatcggatt gaaaagttag catatactga
    22021 tccaatagat caaaaaaaac ttgataaagc aaaagctgaa ttatctaaat tgagaaatga
    22081 aaaaaataaa gaagcgagag aaatattaac gcaagcgata gggttgaaga gtcaaacgag
    22141 aacacgagat gtagcaaaaa aattagactt tgactttgga gattttgttg attccgcaaa
    22201 tatgaaagct agtaaagatt ttggcaatga ttttatcgga attgtgaccg gacaaagaac
    22261 gcctagaata tataaaaata gaaacaaata tggattatta ccttttagag gtcgagatat
    22321 taaaatccat aaagatgata agttttatat ttatttacct aaaggcttta ggtttaaagt
    22381 gaatttaggt agcaagccaa gaaaagcaag agatgttgct tcagttttac aacacattat
    22441 ggatggcgat tacaaattaa atcaaagtaa tcttattttt gatggaagaa aattattttt
    22501 taatctaaca atttcttttg aaaaaagcta taatgaaatg gaattcaaga aaggtaatga
    22561 attgatcgta aactttgcag atttgtgtag cggtgaatgt ttaattaatg ataaatctgt
    22621 aatgtctttt ggtgatagcc aatatataag aaacttcaaa agtaaaattt tagcaatgaa
    22681 atcaaaagaa caaagccgag gagtcttttc taagggcggt catggtcgaa aaagaaaatt
    22741 agatcaagat cgatgggaag caataaagaa aagagaaagt aattttgtta aaacttataa
    22801 taatcaatgt tctgcaaaaa tcgttaaaac tgctattcgc aataaagctc aaattattcg
    22861 tatcattgaa ccagataagg cttatggcga atgggcttac tatcaattga aagaacaact
    22921 agtaaacaaa ggaaaaagat atgggataga agtcagaagt tagtaaatta aacaatcgtt
    22981 gatttggcaa cgaaatagtg agggaaaatc cactcactga agggtaaact ccgtcaagtt
    23041 gtgtgaccag acggaaggac gaatgttgtt gaggttttag aaccatataa aacagcatgt
    23101 ttctcaaatt tttaggataa gggaaattgc tagcagtttg ttttagaacc atataaaaca
    23161 gcatgtttct caaatgagta ctttaaatta attgatggcg ttcctgcttt agaaccatat
    23221 aaaacaacat ggatttaaaa caagaagtaa gtcagttctc cttttacttt agtaaggaaa
    23281 aataaagtat tctcaataaa acttaaaata agcaaacctt tgttttaaaa tgattgtcaa
    23341 gttaaaaaaa tctgggatca tataaaatag tatagttctc aaatatgatt agaaacccta
    23401 ttaaagagca atattaagcc aacttactta aatcgtttaa ttaagaacaa taatctcttt
    23461 tagtcagact tttagtctga cttttttgta tttccaaaat ttattcttta caaaaacgaa
    23521 catacgtttg tataattact tgtgaatggg ggaataaaaa tgtttgatta cagtagagaa
    23581 ccggtgaatg actatttttt aatcgacatg aagtctttct atgctagcgt ggaatgtatt
    23641 gaacgaaatt tagacccaat gactacaccg ttaattgtca tgagtagatc ggacaacact
    23701 ggttcaggtc tgactctagc ttcttcacct actgcaaaga aaaaatatca tattactaat
    23761 gttagtagag ctactgattt accttatcca ttaccaaaag aacttcatat tgttccacca
    23821 cgaatgaact tatatataaa aaaaaatatg gaagttaatc agatttttag aaagtatgtc
    23881 gcagatgaag atttattgat ttattcgatt gatgaatcaa ttttaaaagt gacaaaatcc
    23941 ttaaatttat ttactatata aattgagtta aattgtattc gtttttcctc ctataggcgt
    24001 atactaaaga aaacacttga ggagtgggga acatggaaac agaacgccaa atccgtgaat
    24061 tggaaaaagc gatgaatacg acagacaacc gccggttgta cgagcgtttc ctggcagtca
    24121 agttggttct tgaaggccac acccggaaag aagccgggca caccatcggc cgcgacgagc
    24181 atactgtcag ccactatgtg aaaaactact gccataatgg aatccaaggt ttagtctcca
    24241 aaaaacaaag cggacgacct tcacggctga cagatgaaca ggaagcggaa ctggtccaaa
    24301 tcgtcgccta ccacactccg gaggaagtag gtttcaaatc ccgtgccaat tggacattgg
    24361 cgattgtgtg tgacctgatt ctccgggaat ggagttatgg atatacgcaa aaaggcgtgg
    24421 cggacctcct tcaccggcta ggactaagct ggacccgtcc gacgtatacc ctcaaaaaag
    24481 cggattccaa gaaacaacag gtatttttgg aagaaacctt cccgagccta aaaaaaaatt
    24541 gctgaatagc gatattgacc acctcctatt cgaggacgaa accatgatcc gggattacca
    24601 agccattggc tgcacctggt ttctccgcgg ccaccagcga aaaatcccca cttatggaca
    24661 acacaaaggg gtaaaattga taggcacctt ggattatgaa accggtcgga tcatttgtac
    24721 agaagaaggg gcatacgatg cagcggcctt cctccgtttt ctggagcgga cgttgcggga
    24781 ataccccacc ggaaaaatcg tgatggtatt ggataatgcc cttatccacc acgccaagct
    24841 gatccagtct tttcttgaag aaaacgctag gcgcctggca ctcatcttcc tccctcccta
    24901 cagccctcag ttgaacctaa tggaaggcgt ttggaagtgg ctcaaggaat cggtcatcaa
    24961 caatgttttt tttgaccacg tccagaaaat caaacagtcg gtccgcggtt tcttagccga
    25021 tgtaaacgag cgtccccttg aggtgattga ccgactgtgc gtgcggatgt aaagtattta
    25081 attcaaccta tatagttcag gaacaagaag cgagagaaga agagaattag ctaaaataat
    25141 caaagatgag attcagaaag aaacaggtct aatagcaaca gtcggaatag gagataaccc
    25201 attattagct aaacttgcac tggacaatga agcgaagagt agtaaagaat ttatagctga
    25261 gtggacttat gagactgtac ctgaaaaaat atggaaaata aacgagatga ctgacttttg
    25321 gggcattgga tcacgcatga aaaaaagatt gaatcatatg ggaattatga gtatagaaga
    25381 tttagcaaat tggaatccct acacaattaa aagtcagtta ggtgtaatcg gactccagtt
    25441 atatttccat accaatggaa ttgatcgtac ggatattgct attccaccag aaccaaccaa
    25501 agaaaagtct tatgggaatt cacaagttct accaaaagat tatacgagga gagaagaagt
    25561 agaactagta gtcaaggaga tggcagagca agtagccatt cgaattagac aacacaattg
    25621 caaaactggt tgtgtacgct tagcaattgg tacgtcaatt ttggaagaaa aatcaggttt
    25681 ctcgcaccag atgaaaatac caattacaga taatacgaaa gaattacaaa attattgtct
    25741 ttttttattc aataagtttt atgaggggca ggaagtacga catataggag taacttatag
    25801 cagattagta tacacagatt ccctacaatt agatttattt agtgatccaa gaaaaaagat
    25861 tgctgaagaa aatttggata ggattattga taagattaga caaaaatatg gatttacagc
    25921 tattatacat gcttcatcta tgttagaagg tgcaagaagt attacgagaa gcactttaat
    25981 aggaggacac gcaggaggat taggaggttt gcataatgat tgaatataac gatagaggac
    26041 gtttaaaatg gtcgggattt tatttgtctg atcatactga aagaatcaat gcagacaatg
    26101 tacaacgtgc taatcataat ttggcaaaag aacaaatgac gactgaagag attgcgaaaa
    26161 tactgagaac tgctgtcata aaaaatagac ccgtctgcat tcaaaaagag gaacgaaacg
    26221 ctgaaggtta ctatccagat gatgtagtag gaaaaattat tggatatgac gagcttggtg
    26281 tatttgtgga agaagaaaaa gttcactatg atgaaattag gaatgtttct ttctcggaat
    26341 tattaaaatg gagtcagata tgaacattat taaaaaatac gaattcggtt atattacgta
    26401 tgaacagcta atagaggaat tatggggata tggtcaacaa ttaattaatg aagtggggat
    26461 tgattgcttt tgtttctata ttgaatccgg agcaggatac cataagaaca gatactatat
    26521 atttccatat ccaccaaaat agtctcttag atatttttaa attttctcca aatactttca
    26581 attattgtga aatatttccc tttaattaat caaatttttt ataaaagtaa tatataaaat
    26641 aaattgcttg aagtattata tttagatatc ataatttatg gaagaaaaat taattaaaaa
    26701 ggatgagatt ataatggggt tactggttcc gagagctaat acttatacta tacctttaac
    26761 gaaacgccaa ttaattgcta aaagatatca aaggattact agagccatca atagagagtt
    26821 ttggaattct gaaagtgata ctgctcacag tttatacgta ggttcttatg ggagaggaac
    26881 ggcaattagt actagtgata tagatataat agttgaatta ccgatggcag aatttgacag
    26941 attcaaaaat tatttatcaa atggcccgtc taagttatta caagttataa aaaacgcatt
    27001 tcaggaaata ctaccaaata gtgatattag agctgatgga caagttgtta aaataaattt
    27061 ccatgatgga atcaaatttg aaatagttcc agcttttaat gaaaaagatt attggggtga
    27121 gtcaaaaggg tttatttatc cagattcaaa catgggaggg aactggaaag ctacaaatcc
    27181 caaaaaagag caagaagcca tgaaattaaa gaatacaaaa agtaataatt tattatatgc
    27241 tacgtgtaaa cattttcgac atgtacgaga tacagaattt acgagttatc atctttcagg
    27301 aatagtaatt gacagtttcg tatatgaggc tatgggaaat tggaaattcg ttgaaaataa
    27361 cagtgggggt caaaacatta gttctgtatc ttatgagaca gctttattag aatactataa
    27421 ctcacataaa gttatgggcg gtttaaattt atactctcct ggaagcaatc aatttgttaa
    27481 ttctgatagt agtatcatat gtttagaaaa agtattaaaa aaaatagctt tataaagttt
    27541 tatggaggaa caacatggga gacgaaaaaa agcgcttgga agttcaatta cgtgaagcat
    27601 atggacgagt aacgtatact tacaccactc atttaaagca agccgataat attatttctg
    27661 acaataaaat tttaaggtgt cttcaaattg gtttgtcagc ccttactact ggaggggtac
    27721 ttagtaccat tattactgat aatagtgagt taacctttgt tgctggatta tgttcaacaa
    27781 tttctcttgg attaaactta tactttaaga attttgatct tgctgaagaa gctaaaaatc
    27841 atcaaattgt tgccgataaa ttatggttga tcagagaaga atacgtaagc ttactaacag
    27901 atttaaatcg tttaagtgtt gaagatattg tgaaaaaaag agatcaatta atgaacgaaa
    27961 ctcatgaagc atataaaaat gcccctaaaa caaatagtaa aagctatgaa tcagctcaga
    28021 aagcattaaa atctgaagaa gagcaatttt ttacagaaga ggaattggat aagatgttac
    28081 cagagcatct tagaaaaaag agttctaaat attgaactct ttttttatgc ttaaaattct
    28141 cttgtaattt ttctattcat ctatatttaa gaagaaatat gatgatagaa acatct
//
LOCUS       DACIRY010000029        27521 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.029, whole genome shotgun sequence.
ACCESSION   DACIRY010000029 DACIRY010000000
VERSION     DACIRY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.029"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            155..2344
                     /locus_tag="IYE22_001048"
     CDS             155..2344
                     /locus_tag="IYE22_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9506271.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2341..4134
                     /locus_tag="IYE22_001049"
     CDS             2341..4134
                     /locus_tag="IYE22_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ9506272.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4334..4648
                     /locus_tag="IYE22_001050"
     CDS             4334..4648
                     /locus_tag="IYE22_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ9506273.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4669..5046
                     /locus_tag="IYE22_001051"
     CDS             4669..5046
                     /locus_tag="IYE22_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ9506274.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            5056..5829
                     /locus_tag="IYE22_001052"
     CDS             5056..5829
                     /locus_tag="IYE22_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506275.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5851..7254
                     /locus_tag="IYE22_001053"
     CDS             5851..7254
                     /locus_tag="IYE22_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ9506276.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7436..8620
                     /locus_tag="IYE22_001054"
     CDS             7436..8620
                     /locus_tag="IYE22_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ9506277.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8617..8907
                     /locus_tag="IYE22_001055"
     CDS             8617..8907
                     /locus_tag="IYE22_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506278.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8904..9125
                     /locus_tag="IYE22_001056"
     CDS             8904..9125
                     /locus_tag="IYE22_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506279.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            9167..9946
                     /locus_tag="IYE22_001057"
     CDS             9167..9946
                     /locus_tag="IYE22_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ9506280.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            10013..10507
                     /locus_tag="IYE22_001058"
     CDS             10013..10507
                     /locus_tag="IYE22_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ9506281.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            10563..11201
                     /locus_tag="IYE22_001059"
     CDS             10563..11201
                     /locus_tag="IYE22_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506282.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            11273..11668
                     /locus_tag="IYE22_001060"
     CDS             11273..11668
                     /locus_tag="IYE22_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9506283.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            11652..14105
                     /locus_tag="IYE22_001061"
     CDS             11652..14105
                     /locus_tag="IYE22_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ9506284.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            14102..16129
                     /locus_tag="IYE22_001062"
     CDS             14102..16129
                     /locus_tag="IYE22_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506285.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            16126..17148
                     /locus_tag="IYE22_001063"
     CDS             16126..17148
                     /locus_tag="IYE22_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ9506286.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            17165..18094
                     /locus_tag="IYE22_001064"
     CDS             17165..18094
                     /locus_tag="IYE22_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9506287.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            18339..18455
                     /locus_tag="IYE22_001065"
     CDS             18339..18455
                     /locus_tag="IYE22_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ9506288.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            18471..19274
                     /locus_tag="IYE22_001066"
     CDS             18471..19274
                     /locus_tag="IYE22_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ9506289.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            19449..19745
                     /locus_tag="IYE22_001067"
     CDS             19449..19745
                     /locus_tag="IYE22_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506290.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            19752..21704
                     /locus_tag="IYE22_001068"
     CDS             19752..21704
                     /locus_tag="IYE22_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ9506291.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(21776..22273)
                     /gene="dfrG"
                     /locus_tag="IYE22_001069"
     CDS             complement(21776..22273)
                     /gene="dfrG"
                     /locus_tag="IYE22_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ9506292.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            22635..23675
                     /locus_tag="IYE22_001070"
     CDS             22635..23675
                     /locus_tag="IYE22_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ9506293.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            23773..23961
                     /locus_tag="IYE22_001071"
     CDS             23773..23961
                     /locus_tag="IYE22_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9506294.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(24019..24372)
                     /locus_tag="IYE22_001072"
     CDS             complement(24019..24372)
                     /locus_tag="IYE22_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9506295.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            24903..25325
                     /locus_tag="IYE22_001073"
     CDS             24903..25325
                     /locus_tag="IYE22_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ9506296.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            25322..25552
                     /locus_tag="IYE22_001074"
     CDS             25322..25552
                     /locus_tag="IYE22_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9506297.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            26050..26250
                     /locus_tag="IYE22_001075"
     CDS             26050..26250
                     /locus_tag="IYE22_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ9506298.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            26277..27470
                     /locus_tag="IYE22_001076"
     CDS             26277..27470
                     /locus_tag="IYE22_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9506299.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         9577 a         4424 c         5182 g         8338 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     2461 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     2521 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     2581 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     2641 tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat
     2701 actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat
     2761 gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga agttcagtat
     2821 actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat
     2881 atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt
     2941 gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac
     3001 aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat
     3061 attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa
     3121 atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag
     3181 agattagtag acttctactc taattttcag agttgtcctt acgagattga aagcagaggg
     3241 aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta
     3301 cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat
     3361 atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa
     3421 gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat
     3481 agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga
     3541 agaaagctaa ttgcaaacga tattttaatt atattaaaaa atgaatttgg aatagaagtt
     3601 aaagattatt ttattgactt agatttatta cagctaataa acagtgtgaa aagaatgtat
     3661 agtggagacg acaacggtgt tgatttatac aaatttgtta ccaaacatat atttgttaat
     3721 cttaatattg acgagaaaaa ttacccgtct atttttaagc actatctatt ttttattaac
     3781 gaagtagaag atcggataaa aaataattca ttatctagta gtgtatctag ttttaagaag
     3841 atgtttaaat ctcgagatgg tatcacactt actacagcac ataaagttaa aggaaatgag
     3901 tatgatactg ttatcgctat taatcttcta aacgggaaaa ttcctcattg gaatgaaatt
     3961 tttaatgttt ctgataaagg aattagttca gcgagaaaac tactatacgt aatttgttca
     4021 agagcaaaaa ataatttatt tcttttctca gaggtgggtt attttacgag taaaggagac
     4081 ttgctttatc ccactcaatt attagataaa atagaatttg attataatat ttaaaatacc
     4141 taggtagtcg ttgttttaaa tatagtagga atcggacgct cagtacggag cgtctatttt
     4201 tttacccaat tttgaaagga agtgaactta tgaaaataaa aaatcaagaa tcaaaaggtc
     4261 gctctccccc ctttaaaacc atcacacaaa taagattcat tcaaacataa aaacagaaag
     4321 gataggtaaa aatatggaac ttaaatatgt cattcccaat atggaaaaaa catttggaca
     4381 attagaatat gctggtgaag ggaacattga acaacgtcga ataaatggca gaccaaccat
     4441 tctctctcgt agttacaatt tatactcaac gattcaacga gcagatgata ttgttgtcat
     4501 tcttccctcc gaagctggcg agaaacattt tgaatccgac gaaaaagtca aactcatcaa
     4561 cccacatatt accgcagaag gttataaaat tggcaatcga ggatttacca attacattat
     4621 gcacgcagac gatatggtga aagcataaga aaggaaaggt gaaaaaatat gagattagca
     4681 gaaggtattg tgattgaaaa agaccagaca tttggcacat tgaaattttc cgccttgcgt
     4741 cgtgaggttc gtatccagaa tgaagatggt acggtttccg aagaaattaa agaacgtacc
     4801 tatgacttaa aatcaaaagg acaaggacgc atgatacaag tcagtatccc cgcttctgtt
     4861 cctttgaaag aatttgacta caacgcagaa gtggaattaa tcaatcctgt ggcagatacc
     4921 gtagcgacgg ctacttttcg aggggcagac gttgattggt acgtgaaagc agacgatctt
     4981 gtattaaaag gacagacaaa acctcatcag aaatataaaa aagaagcgtc cgaaagtgaa
     5041 aaataggtgt tgcttatgac aaacacacca ttgattttga aagaaattcc gaaggatgaa
     5101 gccatttctt ttattcggca ataccattat tcaaagatac tccctagatt gtgtaaatac
     5161 tttctaggga ttttttcaga agaaaaatta ttaggcgttg tggaattagg ctggggtacg
     5221 caacccctac aaaccatacg caaactcttt cccgacagtt cccttcaaac cacagattat
     5281 ttagaaattg gaaagatgtg ttttcttcca gaaatgaatc aaaccaatta ttttggcagt
     5341 caagctcttt cagctctaat taagtggttg aaagaacata cggactgtca ttttttatat
     5401 acactggcag atggaattga gggaaaatgt ggctatgtct atcaagcgtc caacttcttt
     5461 tactgcgggt atttcaagac aagtgtctat cgtgataagc agtcatggga aaagatacac
     5521 cctcgaagtg ctcgcctttt attagaggaa aatgctcgct ttgaacaggt agagaaaaag
     5581 cattggcttt cccaggcatt ttgcgaatac aaagggattg agaaaatcaa tggacgcatg
     5641 tttcgctatc tctatccgtt gacaaaagaa gcgaaaaaac tgttaggaca cactctctat
     5701 cgacgtcatt attatccgaa agaaaaaaat ttacgctttg aaaagcgaat cgcttatcag
     5761 aaatatgaag ccatttctca accaactttt gataaacagg ctcgtatcta caatacacaa
     5821 ttattttgat aggtggaaag gaggattttt atgccattgc ttcatgacag aggagagcga
     5881 atcaaagcca acgataagca cttggtctat cattttgtac taatttcatt gctggtagtt
     5941 tttctagtcg tcctactccc ttttcattgg agagcgttac aatcggtgaa ctggcaagca
     6001 gttcagttag atacattttt ctatcaactt catacgccat acttttggag cagtattctc
     6061 actgctcttt ttgtatgcgg acttatcagc tggctttatt atcactatcg tatagattct
     6121 ataaaacagt tagagcaccg tcaaaagtta gctcgtatga ttttagaaaa tcattggtat
     6181 gaagccgaag aaactcaatc agagggattc tttaaagatt tacccagcaa gaaaaccaaa
     6241 caacgaatcc gccattttcc taaaatatat tatcgaatga aagatggact aatccatatt
     6301 catgtagaaa tcaccttagg gaaatatcaa gatcagcttt tgcacctaga aaacaaacta
     6361 gaaacaggat tgtattgtga attagtggaa aaaacgttga aagattctta tgtagagtat
     6421 gtcctacttt atgatactat tggcaatcga attggcattg aagacgtggt agctcaagat
     6481 ggacgtgtca aattgatgga atcggtctat tgggagttcg attctctccc tcacatgtta
     6541 attgctgggg gcactggtag tggaaaatcc tattttattc tcacattgat tgaatctctc
     6601 ctgcatacaa atgcaaagct ttatattctt gaccctaaaa atgctgattt ggcagactta
     6661 ggggcggtca tgaataatgt ctattaccgt aaagaagata tgttggcatg tatcaatcaa
     6721 ttttacgaag atatgattga tcgcagtgaa accatgaaac aacaccctaa ctataaaaca
     6781 ggagaaaatt atgcttatct tggtttgtct gccaattttc ttatttttga tgaatatgtg
     6841 gcaatgatgg atatgctagg tcgtgaaagt accaccgtta ttcataaact caaacaaatt
     6901 gtaatgttag gacgacaagc aggcttcttt ttaattcttg cctgtcaacg tccagacgca
     6961 aaatatttta gtgatgggat tcgggacaac tttaacttcc gtgtggcttt gggtcgtatg
     7021 tctgaaatgg gctttggtat gatgttcgga agcgatacac aaaaacaatt tttcttaaaa
     7081 cccatcaagg gaagaggtta cgtggataca ggaaaaagtg tgattagtga gttttataca
     7141 ccccttgtac caaaaaggta cgatttctta ggagaaatag gaaaagtcat tcaaaaaaag
     7201 caatctgagc ctgtaccgca cgaagtgaaa ggtacaggtt cagacttgct ttgattggtg
     7261 tggcgttagc cacgccaatc ctttagcccc ctctatctaa caggggggca caaatacaac
     7321 tgaaaacagg caattctcat caaccaatgt tgatggtaca aaggattgcg gaaaatatga
     7381 aaggtgtaaa ctcaagggtc atagtttaca tcttttttag attggaggtt tgcgaatgga
     7441 aaacgaaaaa atttggatta aggaattgaa agaaaagcga ctggcttatg gcgtttctca
     7501 aaataaactg gcggttgctt ctcatattac cagaccttac ctatcggata ttgaaacagg
     7561 caaggcagtc cctactcaac aggtgaaaga agatttactg aacgctttgg aacgcttcaa
     7621 tcccgataac ccattggaga tgttattcga ctatgtacgg attcgattcc ctaccaatga
     7681 tgtggctcaa attatcggtg aggttttacg tttgaatatg gattatatgc tacacgaaga
     7741 ctttggctat tattcttatc ccgaacacta tcgtttcggt gatattgttg tcctagtctc
     7801 tcatgatgta agtaaaggtg ttttattaga actgaaagga aaaggctgtc ggcaatttga
     7861 aaactttcta ttggctcaac atcggagctg gtatgatttc tttcaagatt gcatggaaca
     7921 taaaggtatt ttcaaacgct tggatttagc tatcaacgat aaaacaggga ttttgaatat
     7981 tccagatttg gcaaagaaat gtaaacagga agaatgcatt tccgtctttc gaagctttaa
     8041 aaattatcgc agtggtgaat tggttcaccg tgatgaaaaa ccatacatgg gaaacacact
     8101 ttatattggc tcattaaaaa gtgaagtcta tttctgtata tatgagaaag attatgagca
     8161 atacgtgaaa aatgatgttc ccttagaaga cgcagaagta aagaatcggt ttgaaattcg
     8221 gttaaaaaat gaccgagcca cccaagccat gaaagattta ttggcacatc aacaggcgga
     8281 aaaaacagcc tttgaaatta tcaatcgcta tatccgcttt gcagataaag acgacactaa
     8341 acgtcggagt gattggaaaa ctaatgaacg ttgggaatgg tttatcggta aaaatcgtgg
     8401 agcattacgc ctaaccacac agccagagcc ttattctttt gaacgaacat tgaactggtt
     8461 acatcatcaa gtcgccccta cattaaaaat cgcaagcatt ttagatgttc tcaacggtac
     8521 aaccattatc tccaccatga ttcaggaagc caaactgaca gaaaaacatg agaaattaat
     8581 tgaacaacaa catctagcca tggaggattt gattacatga gccttttaac aaaagaatta
     8641 aagaagttag ggtttcagtg tggcattgag tttcaagcgt atatacaaaa tacagggaaa
     8701 tatacgtctc tcattattga gggaaaacgg caagctggtg ataccatcta tacctatgat
     8761 ttttacaagg tgagatttta taataattat accaatcgag taaccgtcta tggagagcat
     8821 ttaacccctt ttcaattatt aaggcgtgta aaaagctata tctattatcg agaaaaatat
     8881 cttaaggaaa ggaggacaat cacatgaatt ttgggcagaa tttgtacaac tggttcttgg
     8941 ataatgcaca gtcattggta ttgttagcga ttgttgtcat tggactgttt ttaggtttta
     9001 aacgagaatt ttcaaaactc attggcttct tgattattgc tctaatcgcc gtcggacttg
     9061 tgttcaatgc agctggcgtg aaagatgtgt tattaaactt atttaaccga atcattggtg
     9121 cttaacagat atttttcatg ataaaatagg aagtatatat tttgttatga aaggatttat
     9181 gaatatgttt acaaatgatc aatttaaatc tcttttaatt aaaacattag aaagaaagag
     9241 ctcttcaaat tattatgaag gaggtaacga gattgtttct aaaatgaaaa tatcagaggt
     9301 aactttagat gagactgacg attttactta ttataaagga tataaaaagg gctggaatac
     9361 gctttgcaca tatcttaaaa taagagttcc ttttgatgat ttagactttt ttgaaagtca
     9421 taaagatttg attacccaga cagcaagctc catatatgat aaacaaggtg ataatgtatt
     9481 agtggatact attttagtac cacttccaga gaattacgaa gtaattaact ttagtcagtt
     9541 gaaaattagt gatgtggttt cacaagctat tgaagacgct gagtctttta tgagtaatgg
     9601 tgaatatcaa cgtgcctttg acagagttca cactgctttt catggatatt taattgaaat
     9661 actaaaaaaa tatgaaatta cagttcctag agacgaaaat ctatctaaat tatatagtag
     9721 gatacaacaa ttaattgaaa aagaaattca acctactgaa cttgctgata tagtgaagac
     9781 aacgataagg tcatcaaatg gaatgattag ctctcttaat gaagcaagaa ataggcattc
     9841 acttgcacac cctaatacaa atattattgg aaagagagaa gcaaaactaa taataggtat
     9901 ttcaagtaca gttacagatt atattagcgg ataccttgat aagtagcgtc attaactgat
     9961 tgctactttt tttatgacta tttttataaa aaggatgtga taagaattgg aaatgaaagt
    10021 ctatgtggca aatttaggac ggtataatga aggtgaatta gtcggagcat ggtttactcc
    10081 accaattgat gaggaagaaa tggcagaaag aatcggttta aatgaggact acgaagaata
    10141 tgcaatacat gattttgagc ttccttttga tgtggacgaa tacacgccga ttagtgaaat
    10201 caaccgtcta tgtgaagcca ttcaagaaat cgaaggtaca cctatttata atgaactcaa
    10261 agaaattcaa ggcatgtggt ttagtagctt ggaagaatta ctggaaaaca aagaggatat
    10321 tcattgttat agtgattgtg attcgatgga agatgtcgcc cgttattacg tggaagaaac
    10381 aggacagctt ggtgaagtcc cttccaattt acaaaattat attgactacc aatctttggg
    10441 acgtgatatg gaaatagagg gtaattacct agttacctct catggggtat ttgaatattg
    10501 tcagtaagtt taacaatccg tgttataatt aaattaataa atagcacgga gaggtataaa
    10561 ttatggtaaa tgtatttgaa aatcttaaac tattaaaact agacatgaaa gataaaggct
    10621 gggtgataga ttccttttac tttcgttaca aacagcaaaa ttatattgta cttgttaagc
    10681 tatttgaaaa agaggaaaaa gttccagaat atgctctgtt aaaactagaa tttttaaagg
    10741 aaaacgattt ttcagatatg ctagctgtct atgctaattc ggtaaaatta tatacagaca
    10801 cgaaaacaat aagagaatat tttggtatcg aatacagtag taacttggga gatgttttat
    10861 ttcaatttag ccaaactctt gctcgattta ttccgacaga agttagtgag aagaaaaatg
    10921 aagaccaaaa agaagccatg tgtttctcat taagtcaaag tgattcagaa gatcccagaa
    10981 aaaaatattg tttttcagtt agaagaaatc ctttaaaggg aaatggtgag ctgggaaaac
    11041 gaagtccttt taatgacaat aaaacgagat tgtatagacc cagcttatat gaacgattag
    11101 ggtcagatac caatttaagt ttcagatatt caatgaatcc agatgatgaa gaaactgatg
    11161 aaggtattat tgcaaaatgg acaaaaaata aaattgaata gttaaagcat tgaaagagca
    11221 atcacctatt ttgattgttc tttttctatt tttacggaaa ggacgtgaag acatgaaaaa
    11281 aataagaagt tacaccagta tttggtcagt agaaaaagtg atttatgcga ttaatgattt
    11341 acaactccct ttcccaatta cctttacgca aatgacatgg tttatcgttt ctttgtttgt
    11401 cgtcatactc tttggcagtt taccaccact ttctctgatt gacggagcac tactcaaata
    11461 cttagggatt cccattgttc tcactgggtt catgtgccaa aagacctttg acggaaagaa
    11521 accctatgga tttctaaagt ctatcattac ctatgctcta cgccctaaag taacctacgc
    11581 tggcaaacaa atacgattaa aaaaagaaac aatgaatgaa tcgattacga ttgtaaggag
    11641 tgaacaacta gatggcatat ccgattaaat atatggaaga taatctcgta tttaatcatg
    11701 atggcgaagt attcgcttat tatgaattaa ttccctataa ttacagtttt ttaagttccg
    11761 atgaaaaaat tcaagttcat gataatttct gtcaattgat tgcccaaaat cgtgatggca
    11821 agattcatgc tctgcaacta gcaaccgaat caagtattcg agccacacag gaacaatcga
    11881 aaaaagaaat cactggacga ttaaaagaga tagcttacga aaaaatagat gaacagaccg
    11941 aagcgttaat ttctatgatt ggtgacaatc aagtagacta tcgctttttt attggtttta
    12001 aactctacct caatgaacag gaagtatccg taaaaaatgt tgggaaagaa attcaaaaag
    12061 catttgcgga attctttcat ggtgtgaatc acaaattaat gggcgatttt gtttctatgc
    12121 ctatggcgga aatcgaacgc ttttctaaga tggaacagtt gttagaaagt aaattatcca
    12181 gacgctttca gattcgtaaa ttggagaaag atgattttgg ttatattatt gaacatcttt
    12241 acgggcaaac aggtgtccct tttgatgact acgactacca tttaccgaaa cgatacgaag
    12301 ataaaaaagt gttggtaaag aaatacgata ttctcaaacc cactcgctgt ttaatcgaag
    12361 aaaatcaacg ctatttacgg attgaacagg aagataccac tacttatgtg gcgtatttta
    12421 caattaatag tattgttggt gaattggatt tccctaatag tgaaattttt tattaccagc
    12481 aacagcagtt tactttcccc gttgatacca gtatgaatgt tgaaattgtc gccaataaaa
    12541 aagccttatc taccgttcgc aataaaaaga aagaactgaa agatttagat agccacgcat
    12601 gggaaagcga caacgaaacc agtactaatg tggtggacgc tttagaatct gtcgatgaat
    12661 tagaaagtaa tttagaccaa accaaagaat ctatgtacaa actaagctat gtggtacggg
    12721 tatcagcaaa gaatttggac gagctgaaac aacgttgtga tgaagtaaaa gatttttatg
    12781 atgatctgtc tatcaaatta gtacgacctt ttggcgatat gctaggcttg cataatgaat
    12841 ttattccctc aagcaagcga tatatcaatg actatattca atatgtcacg tcggattttt
    12901 tggctggtct tggtttcggt gctacacaaa tgattggtga aaattctggt atttacgtcg
    12961 gttacaatct cgatacaggc agaaatattt atctccaacc tagccttgca agtcaaggaa
    13021 ttaaaggctc tgtaacaaat gcattagcgt ccgcttttat tggttcatta ggtggcggaa
    13081 aatcattctc taacaatatg ctggtctatt attcagtact ctttggcgga caggcaacca
    13141 ttgttgaccc aaaagctgaa cgtggtgaat ggaaagaaac cttacctgat attgcccatg
    13201 aaatcaatat tgttaatctc acgagtgaga acgacaataa agggttactt gacccttatg
    13261 tgattttaaa actcccgaaa gattcagaaa gccttgccat tgatattctc acgttcttaa
    13321 caggtatttc cagccgagat agcgagaagt ttcctgtcct gcgtaaagct attcggagtg
    13381 taactcaaag caaacaacga gggttgttat tggtgattga tgaattgcga aaagaagata
    13441 cgcccatcag caatagtatt gctgaccata tcgaaagctt tgtcgattat gattttgccc
    13501 atctgttatt cagtgatggc agtgtcaccc aatccattag cttggaaaaa caactcaata
    13561 ttattcaagt ggcagatttg gtcttacccg attccaatag ctcttttgaa gaatacacta
    13621 ccatggaatt attaagtgtg gcaatgctga ttgtcattag tacctttgct ttagacttta
    13681 tccactctga ccgttccatt tttaaaattg ttgaccttga tgaggcttgg agttttttac
    13741 aagtggcaca aggaaaagct ctatctatgc gattggttcg agcaggacgt gccatgaatg
    13801 caggggtcta tttcgttact caaaacgcgg acgatttact agatgaaaag atgaagaata
    13861 atattggctt gaaatttgcc tttcgttcca ctgacctagt ggaaatcaaa aagaccttag
    13921 aattttttgg tgtggacaag gaagatgaaa acaatcagaa acgcttacgt gaattagaaa
    13981 atggacaatg tttagtttca gacatctatg gtcgtgtagg ggttatgcag tttcacccga
    14041 tttttgaaga gttgcttcat gcctttgata ccagaccgcc tgtgcgaaat gaggtggaag
    14101 aatgacacaa aaacaaaaga aatttcttca ctatagttgg ataacgctcc tttgtgtggg
    14161 cgttatttta cttttattag gcacgctggg caatgcagta caagcgacag gcttagttga
    14221 tgaaaccatt gatacaagca atgaatactc caagtatggt ttgaatcaat atcagcttga
    14281 ttattatgtg gacaatagct ggggctggct tccatggaat tggagtgatg gcataggaca
    14341 atcggtgatg tatggattgt atgccattac taattttatt tggacaattt ctctttatct
    14401 atcgaacgcc acaggttatt tggtgaaaga agcctatgcc ttagatttca ttagtcaaac
    14461 atcccaagca attggcgaaa acatgcagac gattgcaggc attacaccga atggcttttc
    14521 cagttcaggg ttttatgtcg gtttcctcct tctttttatt ctcattgttg gaatctatgt
    14581 tgcctataca ggattggtta agcgagaaac gacgaaagtg atacgagctg ttttaaactt
    14641 tgtcgtggtg tttattctat cggcttcctt tattgcgtat gcacccgatt atattgcgaa
    14701 aatcaatgat ttttccaaag atgtcagtac cgccagtctg aatattggga caaaaattgt
    14761 tcttcctaac acggatagtc aagggaaaga cagtgtggac atgatacgtg acagcttatt
    14821 ctctgttcaa gtaaagcaac catggctatt gcttcaatat ggctccactg atgtagaagc
    14881 gttaggggaa gaacgtgtgg aaagtttatt gtctaccagt ccagatacaa acaatggtga
    14941 agatagagaa aatgtagtga ttgaagaaat tgaagatcat gacaatacct atttaaccct
    15001 accgaagacg atttccagat taggaacagt cttctttttg tttgtattca atattggtat
    15061 ctcgattttt gttttccttc taacaggaat catgattttt tcgcaagtgc tatttattat
    15121 tttcgctatg tttttgccaa tcagtttcct attaagtatg ttgccgagtt ttgatggcat
    15181 gggcaggcat gccattacca agttatttaa tgtgattatg acaagggctg gtattacctt
    15241 gattattacc gttgccttta gtatttccac tatgctttat tcgctgtcag caagttctcc
    15301 ctttttcatg attatgtttt tacaaattgt gacctttgca gggatttatt ttaaactggg
    15361 cgatttaatg agcctattca ctttacaaag caacgactct caaaacatgg gcagacaagt
    15421 gtttcgcaga cctcgtcaaa tgatgaatcg acaaaccaga agatttcaac gcagtatgag
    15481 taagattttt cgtggaaaag cagggtcaac gaagcaaaag acaatgacaa caaagcagaa
    15541 tcaaaccaat aaagctaacc atactcgtca aaatgaacgg actacaccaa agaaagaaac
    15601 agcactttcc aatgtgaaaa agcaacaagg cggtatcggg caacgattag gtgaaaagac
    15661 agcccatgta ttggacacga aaaatcgtct agtcaataaa agaaagcagg caaaacaaca
    15721 gatacaggcg acaccaaccc atgtcaaata tgcccttcat caaggaaaag agaatgtcac
    15781 tcataatgta acagacttta aacaatctat ggcagatacc aaagctcaac gcaaacaaca
    15841 gcgtatgaac cagcaggagc aaaaacgaaa gaacattgct caaaagcgtt tggagctgga
    15901 taaagcaaaa gaaaaaagac gaaaagaaaa cccatcatct cctgttcaga ataaccagca
    15961 gcatgttcgc aagcgtcagc aaaccgtcac gcctgttttg tcacaaaaca ctaaacgtga
    16021 aaatcatcag cgaccattga aaaaagagca aaaacaaaag acaccaccga agcaattcaa
    16081 tcagaggaag ctaaataaaa gaacggataa gcgaggacaa caaacatgaa gctaaaaata
    16141 acagtgattt gtagtgtggt cttctctctc ttcttttttc tcttattatt cctagtcatt
    16201 ttctttgcag atgatacgga cagtggcgaa aacaacaagg attcttctat tccacaaggc
    16261 ggtgtgaccg tttcaccaga agtgttggca caccgtccgt tgattgaaaa atatggtaag
    16321 gaatatggga ttgaagacta tgtttcttat atcttagcca ttatgcaagt ggaatcgggc
    16381 ggtacagcag aagatgttat gcaaagttcc gaatctttgg gtttaccgcc caacagtttg
    16441 agtaccgaag agtccatcaa acaaggggtt aaatatttca gtgaattatt aaccagtgca
    16501 gaacaacaag gagtggatat agattctgtt atccaatctt ataattatgg cggtggcttt
    16561 ttaaattatg tgaggagtca tgggaaaaaa tatacctatg aattagccga acagttttct
    16621 aaagagaagt cgggtggtca aaaagcagac tatcccaatc ctatagccat acctgtgaat
    16681 ggtggttggc gatataacta tggaaatcag ttttatgttc agttggtttc acaataccta
    16741 accgacactt ccccaacaga atttgatgat gaaaccgtgc aagtcattat ggacgaagcc
    16801 ttgaaatacg aagggtttcc ctatgtcttt ggaggggcgt cgcctaccac ctcttttgat
    16861 tgtagtggct taatacaatg ggtgtatgac aaagctggca tttctctccc tcgtgtcgcc
    16921 caagatcagt atgacgctac gcaagaaatc tctatggaag aagcccaagc aggcgacctt
    16981 atcttctttc attcaaccta taatgcaggc acatatgtga ctcatgtggc gatatattta
    17041 gagggaaatc gcttttatca tgcaggcgac ccaatcggtt atggcgactt aagcagtcgt
    17101 tattggcaag accatctgat tggggctaga cgtgttattc ataactaaga aaggaatgaa
    17161 tcaaatgatt ataaaaatcg agcgaaaaga aaagaaggag aaaccaaaga aatctaaaaa
    17221 accgagcaag caaagaaaag ttccgatgat taaagttggc acgcataaga aattaacctt
    17281 tgttttatgg gttttattga ttggtagtgt cggatttgga atctataaaa attttactgc
    17341 cattgacact catactgtcc gtgaaacaga aatcattaaa cagcagattg tcgataccaa
    17401 ccaagtagaa agttttgtga aatcctttgc gaaagactac ttttcgtggc aacaatccca
    17461 agaagccatt gataaacgaa atgaaaaatt gacccattat ttgacggaag aattgcaggt
    17521 attaaatgaa gaaatgattc gtaaggatat tcctactagt tctagtgtga acgatataca
    17581 ggtgtggcag gtttctcaag tgaatgagaa cacctttgag gtactgtttt ctgttgaaca
    17641 agttattacc gaagacaaag acaaggaaac tatatcgtct agctttcatg tggttgtcca
    17701 tatagatgaa tcagataata tggttattat caaaaatccg acaatgagta agaaacctca
    17761 aaaatcggac taccaaccca aacagctaga aagtgaccat acagtagata cagaaacgat
    17821 ggacgaaatc actagctttt tagaaacgtt cttccagctc tatcctaccg ccacagaaaa
    17881 agaattgact tactatgtaa gtaatcatgt gttacctatg attaacaagg aatatgtttt
    17941 cgaggaattg gtgaatccta tctttaccag aaaagataac caagtaatcg tgaatgtcgc
    18001 tgtgaaatat ttagatcaag aaacaaaggc aacccaaatc tcgcaatttg agttgatatt
    18061 agaaaaacaa gataattgga agattgtaaa ataacaaata ttggtacatg attacagata
    18121 ctttgtaatc atgtactctt tttgataaaa aattggagat tcctttacaa atatgctctt
    18181 acgtgctatt atttaagtga ctatttaaaa ggagttaata aatatgcggc aaggtattct
    18241 taaataaact gtcaatttga tagcgggaac aaataattag atgtcctttt ttaggagggc
    18301 ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag tatccagaga
    18361 atatctgtat gctttgtata cctatggtta tgcataaaaa tcccagtgat aaaagtattt
    18421 atcactggga tttttatgcc cttttgggtt tttgaatgga ggaaaatcac atgaaaatta
    18481 ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca gaaagcttat
    18541 tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg
    18601 ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc
    18661 agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag
    18721 aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg
    18781 gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa
    18841 tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata
    18901 ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata
    18961 tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg
    19021 accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg
    19081 aaagcataag atttcagaat tgttctctgt tccctcttta tcatggaagt gcaaaaagta
    19141 atatagggat tgataacctt atagaagtta ttactaataa attttattca tcaacacatc
    19201 gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa aaaagacaac
    19261 gtcttgcata ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta aacctaaatg
    19321 agtatcaaat tgcaactgaa tttgatactc gttttttatt gttaaatcaa cgtttgttgt
    19381 taatttacac cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt gaaataatta
    19441 ttgtaggaat gcaaattggt gtgttaaaat ggttacaatt tactaagcaa aaaggggtgc
    19501 attttcctat gcaatttctt gagccaaaaa ataagaacgc taagtctgtg gattgggaaa
    19561 tatcagagca agtaagggtg atagttaagc aatacgctga atatgcagag cgtactgaaa
    19621 gcgaagcagt ggatgaattc ttattgaaca ttttagacga taagaagttt attgaatgga
    19681 ttgccaacaa gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa gatagagtgg
    19741 gttgatagac aatgaccaag cttaaaagat tagccacgaa ggaagatgaa attgtagatg
    19801 ttaaaattcc gatttcaaac gaagaattaa aagatagggc taaacaatat gatttattaa
    19861 ctccaaaacg atttgcaaca aggtataaca aaatgttgtt cttgccaacg tcttttaaat
    19921 ggaacggttc tgaatatccc attcaatata attactgcat caatccgttt tgttgtaatt
    19981 ttgggaaaga acagcacaaa ttcaaagatg taaaagggaa accaagtcga tataaaatga
    20041 ctggtagttc caaagataaa ggtcataaag gtatgtactg caatgacaat cctattggca
    20101 gaggtgtatc gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt gttgaagaaa
    20161 ttaaacgcct tatagaaatc aatagcatcc aagatgttga gccagattat caatttcata
    20221 aagaaggctg ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa ttttacaaaa
    20281 gggggaaaag taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag aagttcacta
    20341 atgttctacc aaaaagagaa gaaaccacta cttatcacca acaaaagaac accattcttc
    20401 caatgttcgc taagatggtt gttggaaggg tttcagtcag tcgtacttgt gatattttag
    20461 gaattggagt tggaacctac tatcataagc tggagtggct gtataggcgt tgtttggagt
    20521 ttttagaacg atatgagaca caaccccttc aaacaaagaa atttaacgag atgtggctta
    20581 atacggacaa gatgcactac tacctaaata acgtccgtaa gaaaggacaa ggttcaaaga
    20641 aatatacggg gtttgaagac cttaatatgc aaacgtatat agttgtatct gctgaggtct
    20701 tatcaagata tgtgttccgt tctgatgttg cctatgattg gaatatttca atggatgaat
    20761 taaatgaaga tacaagaaaa ttcaaagaag accatctcaa tacttttagt cgtaaaaatg
    20821 ataggttaga ttggtcttat tatccacaag aaccatccgc taacgatagc gaaaatagga
    20881 atgcttatct tcacgaatta gggaaaatta ctaataggag tagatttgtc gatggtttaa
    20941 atgttgatgc tccttatact acaactgctc actattggtt aattaaacaa atggttaatg
    21001 ccgacgaatg gcgaatgatt agtgatgatg acttctctat tagaaacgcc ttttatagag
    21061 tattcacgaa agaattaagg ttgagcgatg cacatcattt tatttgccaa gttaacaaga
    21121 cgaaaagcag aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa ctgctcgatt
    21181 ggggagatat cagaggattc aaaacaaagt ttctacggac tttggcatct cattatctaa
    21241 ctgaattact aacaagtcat caattccacg aagaggctat tagtaaagat ggagaaagat
    21301 atcgcaaata tgccgataac cctatcaaac atccattagc cactaaggat aaaggatttt
    21361 attcagttga ttgtagaacg gatttatctg ctctggaacc aaacgaaatc gcaaagatgc
    21421 tattaaatgt taatgaccat tcgaccaata gctttattca acagataaga cgttatatat
    21481 catctcttga aagaccatta acaacagctc gtggtgataa gaagagctat atttacgcta
    21541 acttcaatcc gaagtatgct caatttgcga ttacaattct aagaacttac tacaactttt
    21601 gtcgcccttt taaatcagcg gataaaaagg ttttgacacc tgctcaaaga ttagggatta
    21661 cagataaaca atttgattgg aaagatataa tttatttcaa atgacaaaaa gagcttggag
    21721 taatcccaag ctcttgtgct ttatctttac tgttcttctt caattaacgc acccgttaac
    21781 tcaataagtt ttttctttca tatacatgaa aatagtagtt atacggattt ttatcattct
    21841 ttatcccttt ttgggcaaat acctcattcc attcctcata attcacttct ggaaaaaaag
    21901 tatctccttc gaattcatga tgtatttttg tgatgtacat tttctcaaca taagggaaaa
    21961 acaaattata aatctgttct cctccgaaaa taaaaatttc ttcttcgttt ttacataact
    22021 caaaaacatc ttctattgaa tgaacaattt cacaaccatt aaaggtaaac cccttatctc
    22081 tcgtcagaat aatatttctt ctgtcaggta aggctcttcc gattgattca aggttcttcc
    22141 tacctaatat tatcggatgt ccctttgtag tatttttaac atattcccag tccttgggaa
    22201 tcctccaagg aatgtcattc tctttgccaa tcactctatt cttatccatc gcagcaatca
    22261 aagaaacttt catttataac acagcctttt taactaattt accatctaat ttcttatttc
    22321 tatcaaaaaa ccttttctca ttatcctgcc cgttagttga ataagcaatt ttttctataa
    22381 gttatattat cataatacca caaaatcaca aattttcatt caattatcat tttcaattgc
    22441 attttataaa tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca
    22501 ttttgaagta cattttgtca ttttgtactc caaaactgaa ctacttactt gcatatatac
    22561 gcctttatag tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa
    22621 taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt
    22681 attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag
    22741 atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa
    22801 ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct
    22861 cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt
    22921 ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg
    22981 tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag
    23041 aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg
    23101 tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact
    23161 taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat
    23221 tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtacggttta tactatagcc
    23281 ctgttagtac tccagcagat tttcggatgc ttactcctat tgtactggag caagccttta
    23341 gaaaagctgg aacagaattg ttagagccat atcttagttt taaagtttat gcaccacagg
    23401 aatatctttc acgggcatat aacgatgctc ccaaatattg tgcaaatatc gtaaatactc
    23461 aactgaaaaa taatgaggtc attattattg gagaaattcc tgctcgatgt attcaagatt
    23521 atcgcaatga tttaactttt tttacaaatg ggcttagtgt ttgtttagca gagctaaaag
    23581 gatatcaggt taccactggc gaacctgttt gccagacccg tcgtctaaat agtcggatag
    23641 ataaagtaag atatatgttc aataaaataa cttagtgcgt tttatgttgt tatataaata
    23701 tggtttctta ttaaataaga tgaaatattc tttaatatag atttgaatta aagtggaaag
    23761 gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca
    23821 agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt
    23881 aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa agagccagac
    23941 gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg ctctttttgt
    24001 ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac cttttgctag
    24061 tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt ctacctgtaa
    24121 ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa tagatacatg
    24181 gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt tgttttctat
    24241 atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac gagtgagacc
    24301 ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt atttatcttc
    24361 ttttttacgc atactaaatc acctctatac attttactgt tcctatataa ccataaacag
    24421 gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg aatttattaa
    24481 tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat acaactttat
    24541 aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga gggaaaagtc
    24601 gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc aaaaaaactt
    24661 atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac atagatagaa
    24721 aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg tcacttaata
    24781 aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg tcggggttta
    24841 gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag ggggtgaaaa
    24901 gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac
    24961 aagttattga agatgaacga aaagattact tcaaacatct agacagacta tcagcaaaag
    25021 aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact gttgatacgt
    25081 acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt gaaagtgatt
    25141 tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt ttacttcatt
    25201 actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat cgttcgactg
    25261 tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag
    25321 aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga
    25381 cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt
    25441 acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa ccctacgtgg
    25501 cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat agtcccattt
    25561 tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt tgttattcca
    25621 tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac gttctttgac
    25681 aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc
    25741 atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc tttagcagga
    25801 ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata gtccgagcgt
    25861 tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt gaaattcctg
    25921 tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt gtattattat
    25981 aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat aattgggaaa
    26041 ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata tggaacttct
    26101 ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg gcatatctac
    26161 tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat tctaggaatt
    26221 cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta aaaactatgg
    26281 ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca aatctaggtt
    26341 atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact aaaaaagaag
    26401 caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt gagaataagg
    26461 ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat aagacacagg
    26521 taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc tcttattttg
    26581 ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa ctaaaattat
    26641 caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca gttgctatgg
    26701 atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt ggcaatgtga
    26761 aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa gtaatctctc
    26821 taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg tttttattta
    26881 tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt gattttgact
    26941 caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat tatagatttg
    27001 ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc acattaactt
    27061 atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt gtaatgagct
    27121 ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc tatgctaagc
    27181 tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct tccttgttaa
    27241 tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa gatattgaga
    27301 cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta gctaacaaat
    27361 taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca ccaaagaatc
    27421 aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta
    27481 aaaagataag aagagccaga aacccttggt ataagaggat t
//
LOCUS       DACIRY010000030        26283 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.030, whole genome shotgun sequence.
ACCESSION   DACIRY010000030 DACIRY010000000
VERSION     DACIRY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.030"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(141..1439)
                     /gene="eno"
                     /locus_tag="IYE22_001077"
     CDS             complement(141..1439)
                     /gene="eno"
                     /locus_tag="IYE22_001077"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ9506300.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1555..2310)
                     /locus_tag="IYE22_001078"
     CDS             complement(1555..2310)
                     /locus_tag="IYE22_001078"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ9506301.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2414..3604)
                     /locus_tag="IYE22_001079"
     CDS             complement(2414..3604)
                     /locus_tag="IYE22_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ9506302.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3714..4715)
                     /gene="gap"
                     /locus_tag="IYE22_001080"
     CDS             complement(3714..4715)
                     /gene="gap"
                     /locus_tag="IYE22_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ9506303.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4756..5793)
                     /locus_tag="IYE22_001081"
     CDS             complement(4756..5793)
                     /locus_tag="IYE22_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ9506304.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(5996..7330)
                     /gene="rpoN"
                     /locus_tag="IYE22_001082"
     CDS             complement(5996..7330)
                     /gene="rpoN"
                     /locus_tag="IYE22_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ9506305.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7390..7461
                     /locus_tag="IYE22_001083"
     tRNA            7390..7461
                     /locus_tag="IYE22_001083"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7423..7425,aa:Arg,seq:ccg)
     gene            complement(7653..8537)
                     /locus_tag="IYE22_001084"
     CDS             complement(7653..8537)
                     /locus_tag="IYE22_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ9506306.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8624..9523)
                     /locus_tag="IYE22_001085"
     CDS             complement(8624..9523)
                     /locus_tag="IYE22_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ9506307.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9540..10046)
                     /locus_tag="IYE22_001086"
     CDS             complement(9540..10046)
                     /locus_tag="IYE22_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ9506308.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10235..10855)
                     /locus_tag="IYE22_001087"
     CDS             complement(10235..10855)
                     /locus_tag="IYE22_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ9506309.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10931..11368)
                     /locus_tag="IYE22_001088"
     CDS             complement(10931..11368)
                     /locus_tag="IYE22_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ9506310.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11531..12361
                     /locus_tag="IYE22_001089"
     CDS             11531..12361
                     /locus_tag="IYE22_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ9506311.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12345..13712
                     /locus_tag="IYE22_001090"
     CDS             12345..13712
                     /locus_tag="IYE22_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9506312.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13731..14540
                     /gene="yidA"
                     /locus_tag="IYE22_001091"
     CDS             13731..14540
                     /gene="yidA"
                     /locus_tag="IYE22_001091"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ9506313.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14583..15026)
                     /locus_tag="IYE22_001092"
     CDS             complement(14583..15026)
                     /locus_tag="IYE22_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ9506314.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15160..15531
                     /locus_tag="IYE22_001093"
     CDS             15160..15531
                     /locus_tag="IYE22_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ9506315.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15700..16482)
                     /locus_tag="IYE22_001094"
     CDS             complement(15700..16482)
                     /locus_tag="IYE22_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ9506316.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16479..17450)
                     /locus_tag="IYE22_001095"
     CDS             complement(16479..17450)
                     /locus_tag="IYE22_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ9506317.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17542..18936
                     /locus_tag="IYE22_001096"
     CDS             17542..18936
                     /locus_tag="IYE22_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ9506318.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            complement(19128..20483)
                     /locus_tag="IYE22_001097"
     CDS             complement(19128..20483)
                     /locus_tag="IYE22_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ9506319.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(20513..21148)
                     /locus_tag="IYE22_001098"
     CDS             complement(20513..21148)
                     /locus_tag="IYE22_001098"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ9506320.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(21155..22531)
                     /locus_tag="IYE22_001099"
     CDS             complement(21155..22531)
                     /locus_tag="IYE22_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ9506321.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(22524..24305)
                     /locus_tag="IYE22_001100"
     CDS             complement(22524..24305)
                     /locus_tag="IYE22_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ9506322.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMCG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(24327..24638)
                     /locus_tag="IYE22_001101"
     CDS             complement(24327..24638)
                     /locus_tag="IYE22_001101"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ9506323.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            complement(24628..25614)
                     /locus_tag="IYE22_001102"
     CDS             complement(24628..25614)
                     /locus_tag="IYE22_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ9506324.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            complement(25629..26213)
                     /locus_tag="IYE22_001103"
     CDS             complement(25629..26213)
                     /locus_tag="IYE22_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ9506325.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
BASE COUNT         7378 a         5596 c         4660 g         8649 t
ORIGIN      
        1 aaacatcata tacagaagct agttgcaaaa aaactctcta cctgctgata tttcagcaag
       61 tagagagttc gattacttaa ctaagtgatc atactcgact atctttcaat agtcgatcat
      121 gtatcttcat tagaaaagaa ttatttgttt tttaagttgt agaaagattt caaacctttg
      181 tattctgcaa cttcaccaag ttggtcttcg atacgtagta attggttgta tttagcaata
      241 cggtcagtac gtgatagaga accagttttg atttggccag cgtttgttgc aacagcgata
      301 tcagagattg ttgaatcttc tgtttcacca gaacggtgag atacaactgc tgtgtagcca
      361 gcttctttag ccatttcgat agcttcgaaa gtttctgtta atgtaccgat ttggttcact
      421 ttgataagga ttgagttagc aattcctttt tcgatacctt cagataattt ttgagtgttt
      481 gttacgaaca agtcgtcacc aactaattga actttgtcgc ctaatacgtc agttaatttc
      541 ttgaatccgt cccagtcgtt ttcgtctaat ccatcttcga ttgagatgat tggatattta
      601 gaaactaatt cttcgtagaa tttgatcatt tcgtcagttg ttttttcgcc ttcgcctgag
      661 tcagctagta cgtaaacacc tttttctttg tcgtagaatt ctgaagaagc agcatccata
      721 gcaagaacaa cgtctttacc aggtacatag ccagcttttt cgattgcttc gatgattact
      781 tcaaaacctt cttcgtttga accaaggttt ggagcaaatc caccttcgtc accaacagaa
      841 gttgctaatc cgcgagcttt caagattgaa gctaatgcgt ggaatacttc agcacccata
      901 cgtagagctt ctttgaatgt tggagcgcca acaggcatga tcatgaattc ttggaagtcg
      961 attgagttat cagcatgtga tccgccgttg atgatgttca tcattggtgt tggcaatact
     1021 tttgtgttga atccgcctag gtagtggtat aaaggtactt ctaggtagtc agcagctgca
     1081 cgtgcaacag cgatagaaac acctaaaata gcgttcgcac ctaatttacc tttgttagga
     1141 gttccgtcta aagcgatcat agctttgtcg atagccattt ggtcacgaac atcgtagcca
     1201 atgattgctt cagcaatgat gttgtttacg ttgtcgacag ctttagttac acctttaccg
     1261 ccataacgag ctttgtctcc gtcacgtaat tcaactgctt cgtattcacc agttgaagca
     1321 cctgaaggaa ccattccgcg gccaaaagct ccgctttctg tgtatacttc tacttcgatt
     1381 gttgggttac cacgtgagtc taagatctcg cgagcgtaaa catcagtaat aattgacatg
     1441 ttttgtctct cctttgagtt tgttttttcc taagggattt ccctcatata cgattttagt
     1501 taattttgct cattagtgca aacatttctt gcatttttcg caaattttca acaattattt
     1561 tacagcgtct aacaatgaca agaatgaatc tgcttgcaag cttgcgccac cgactaaagc
     1621 accgtcaaca ttttcttttg ccatgtattc agcaatattt tctggtttta cagaaccgcc
     1681 gtattggata cgaactgctt ctgatacttc ttttccataa agtttttcaa ctgtttgacg
     1741 tacaactcca caaatttcgt cagcgatttg tgcgtcagct gatttaccag taccgattgc
     1801 ccagattggt tcataagcaa taaccatgtt tgacacctgt tcagctgaaa gatcagctaa
     1861 accagctgta atttgtcctt cgatccattc agcagttttg cctgcttcat aggtttctaa
     1921 agattcgccg cagcatagga ttggtgtcat accatttgca aagatagctt tcgctttttt
     1981 attgatgtct tcgtctgttt catggaaata ttcacgacgt tctgaatgtc cgatgatcac
     2041 ataatgaaca cctagatcag caagagctgc tggtgaagtt tcaccagtga acgcacctga
     2101 gttttcccag tagcagtttt gtgctgagat ttttagttct gtaccttcag ctgatgtcat
     2161 taattcttgt aagaataatg caggagatcc gatgactgaa tcaactttgt catttgaagg
     2221 gattttgttt tttactgctt cagcaaattc ttttgcttct gatgcagttt tgttcatttt
     2281 ccagttacct gcgataatag gtttacgcat tgaggaacac actccttaat ttattgaaaa
     2341 atggctaggg agtcatgaaa actcccttca ccttttgttc gattactcgt cagaattgag
     2401 caaacttggt cgtttattta tcgttgattg ctgctagtcc tggtaattct ttaccttcaa
     2461 gcaattccaa gcttgcgccg ccacctgttg agatgtgtgt gaatttgtca gcaaatccta
     2521 attgttctgc tgctgcggct gagtctccgc caccgatgat tgttgttgcg ccttctaagt
     2581 tagcgattgc ttcacaaaca ccgattgtac ctttagcaaa gttactcatt tcaaatacac
     2641 ccataggtcc gttccaaaca actgtttttg caccttgtaa tgtatcagca aataatttta
     2701 ctgatgctgg gccgatgtct aaagccatca aaccatcagg aatgtctcct tcgattactt
     2761 gtgtttcaac atcatttgaa aattctggtg cacagacatt gtcgattggc aagacaagtt
     2821 tgtcgcctgc tttttcgatt aattcttttg ctagttccac tttatcagct tctactagag
     2881 agttaccgat cttgattcct tttgcttcgt agaatgtgta agtcattccg ccaccgatca
     2941 agattttatc tgctttagga atcaagtttt cgattacgcc gattttatca gaaacttttg
     3001 cgccaccaag gattgcaatc attggtcgtt ttggttcttc tactgcttca ccgatgaact
     3061 tgatttcttt ttccatcaag aatccagcaa ctgttgggat accagttgaa gcgataccta
     3121 cgttagaagc atgtgcacgg tgagctgtac cgaaagcatc gttaacaaat acatcgccta
     3181 atgaagccca gtatttacct agttctgtat catttttgct ttctttttta ccgtcaatat
     3241 cttcaaatcg tgtattttcg aatacgacaa cgtcgccgtc attcatgttg cggattgctt
     3301 cttctaattg ttctccgcga gtttcaggta cgaatgttac ttctttacct aataattcgc
     3361 ctagacgttt tgcaactgga gctaaagatt ttccttcttt atcttcttct gtttttacac
     3421 gtccaaggtg agagaaaagg attgcttttc cgccttgttc tagaacgtag ttgattgttg
     3481 gcaatgctgc tttgatacga gtgtcgtcag tgatcacgcc gtctttcaaa ggaacgttga
     3541 agtcaacacg gacaagaact tttttgtctt ttaaatcgat atctttcact gttttcttag
     3601 ccattttatt tcctcctgaa taaacttatt atcaaaaaaa gcggaagaag cgcgattgct
     3661 tccccgcttt taaatgtaca tgtctataat ttacacttgt acgtttgtct atcttataag
     3721 ttagcgaagt attctaaagt acgtactaat tgtgcagtgt atgacatttc gttgtcgtac
     3781 caagcaacag ttttaactaa ttgtttgtcg ccaactgtca ttactttagt ttgtgtagca
     3841 tcaaataatg aaccgtaagt cataccaacg atatctgaag aaacgatttc gtctgtgttg
     3901 tatccgtaag attcgtttga agcttctttc attgctgcat tgatttcgtc aacagttact
     3961 tctttttcaa gaactgttac taattctgtt aatgaacctg ttggtacagg aacacgttga
     4021 gcagctccgt ctaatttacc gttcaattcg ggaattacta aaccgatagc tttagcagca
     4081 cctgttgagt taggaacgat gtttgcagca gcagcgcgtg cacggcggaa gtcaccttta
     4141 ggatgaggtc cgtctagagt catttggtca cctgtgtaag cgtggatagt tgtcattaat
     4201 ccttcaacaa caccaaattt gtcgttcaat gttttagcca taggagctaa acagttagta
     4261 gtacatgaag cacctgagat aactgtttct ttaccagtta atgtttcgtg gtttgtgtta
     4321 taaacgattg ttggaacatc gtttccgcca ggagcagaga taacaacgcg tttagcacca
     4381 gctgttaaat gtttttcagc tgctgattta gaagtgaaga aaccagtaca ttctagaacg
     4441 atatctacgc ctagttcgcc ccatggtaat tcttctgggt tgcggttagc taatacttta
     4501 acttcttttc cgtttacgtt gaatgaacct tcatgaactt caactgttcc gttgaaacgt
     4561 ccttgagttg tgtcgtattt taacaagtgt gccaacattt tagcatctgt caagtcgttg
     4621 attgctacta cttcgattcc atctacatct tggatacgac ggaatgctag acgtccaata
     4681 cgtccaaaac cattaatacc tactttaact gtcatttaag atttcctcct tatgaaaatc
     4741 aaaaaaatat ttattttaaa gggttacccc ttttaaaatc tcatttgcag cagcttcatc
     4801 agtgatgagc cacgtttgtt ttggtgcatt tttcatataa gctcgaattg ctttagcttt
     4861 tgattttccg cccgcgattg ccatgatgat cgggactttt tccaaatctt gcaattctat
     4921 gccgattcta gggaccttat aaacgacttc acctttttca ttgaaaaagt agccaaaaga
     4981 ttccgcaaca gcatttgctt gtttcagcat gactaattct ttttcagtca tcttacgacg
     5041 tgctgccatg tgtaatgcac gcccaatact atgaataaca caattcgctt cactaatcaa
     5101 gtttaagact tcttggatag atggctcatt gagtaaagaa ttgtaagttg ccagacttaa
     5161 ttgttccgga acataaagtg cgcggtggtt accgtttgct ttagcagcca ttcttgcact
     5221 gacagaattt gcctgtacag ttatcgcttc accaatcccg cctcttgcag ggacaaacag
     5281 gttatgccgt ttcttatttt ccagattaga caattgttcg gctaccacag ccatagtagt
     5341 tcctcccatc acagcgatga tattctcgcc ttctggaagt agtcggtcta aggcttcgct
     5401 cagcgcttcg ccgaatgcat cagccacttt ggtctgctct tcgctgtttc ctgaaacaac
     5461 gatacagcgt tggttaccaa aatattctgc caactgttgt tcggtttgat gcgtacctaa
     5521 aagctgatcc atgaagtttt caaggctttg ataaacttct tcgcccttag ttgtcagcgt
     5581 cataccgctt tttgaggtgt cgatcaaatt cagatttttc aaaaggtctg tttctgttcg
     5641 taagactcgt tctgtgatcc ccatactttc cgataaactt cgacgcccaa tcggctgcat
     5701 ccaatagata tttcgtaaaa ttttatagcg ttcttgcaac acttcgataa tatctggtgc
     5761 aacagattca atcattttta tttcatccag catagcgatc tccttagttg gacactttac
     5821 gtccaatgta gacttaaaac gacccatata cgataaaaaa agaaattaac tattcgaatt
     5881 tcacatcaat aaagggaaat ctcaacctca cgactgtaag tctaacagtt ttgctctcgt
     5941 ttttcaacta aaaagactag aaaaagtgaa atactttttc tagtcttttg actgattagt
     6001 tatcataacg ttttcttttt gatgaactcg ggattcctaa taaatcacga tattttgcta
     6061 ctgttcgtct tgagatcgct atctcatctt ttttcagcaa ttcaaccaat ttctggtcag
     6121 acaacggctt gtttttgtct tcttgatcga ctaattcctg cagctttttc ttcgctgtat
     6181 ctgctgaaag gtcttctgtc tgttctgtac tattcgtagg aattcgtgta gtgaaaaatt
     6241 tttttaattc aaatacaccg aagtctgttt ccaaatattt cccgttgact gcacgactga
     6301 ccgtggattc atgcacatct atctcagttg caacttcctt taacgtaaga ggcttgattg
     6361 gacgttcctt gtcgataaag aaagcttttt gccgggaaac aatgacttga gcaacccgca
     6421 aaattgtatc cccacgttgt aggatcgttt tcttcaacca ttcgaattcc tgtttccgtt
     6481 cttttaaata attttcagtt tctttatctg cttgttgtga catttgtttg aagtaactct
     6541 cttgaaagcg tatatttggc tgattttttc ggtttgagat aacttgtaca tttttatcgt
     6601 cgtccactaa tacccggaca tcaggaataa tgtacaattc gctgctacgg tcgaaaatcc
     6661 ggccagggct tggactcaac gtttgtaaat agtcgaatat ctgctgaaca gcatgtagat
     6721 caacagaaaa acgttgtgcg atttctttcc attttcgctc caccagttcc tcaaaacatt
     6781 cttccaacac gatataagct aattccggcg cataatcatc tcgttctgtt tgaagcatga
     6841 gacattcttg caaactacgt gcacctactc cagctggttc taattgctga atcagagtca
     6901 acgcatcgag catttggata ggcgtagctc caatctcttt tccggcttct tctaaactaa
     6961 tcgtcaaaaa tccgttcaaa tcgatgtatt ctacaagata aagaacgatt ttcctcaaaa
     7021 aagtatctcg gtaattcaaa tggacttgtt cgatcaatga atcaaataaa gacccttttg
     7081 tatcaggaat ctgatttagg taatttgtct cttctccttc ataagaagat gaagaagctt
     7141 ttatgtaatc tggctgccat tccggctcaa caacttccac caatggattt tccattgctt
     7201 gattctcaac gaatgccaac agctcctcac tgttatattg taaaacttgg atggactgtt
     7261 gcaactgttg cgtcatggct agtttctgtg tctgctgctg tttttgtgaa aaattttgct
     7321 caaatttcat ttttttgtcc aatccccttg taatttttta tctgtttagt tacaattatc
     7381 ccagtgcttg cgtccttagt gtagtggata tcacgtaaga ttccggttct tgagacgggg
     7441 gttcgattcc ctcaggacgc gttctactgc tattttagca taatttacga atagttgctt
     7501 ctttattttt aagtgaaaac tgtccgccaa agaaaattgg tggacagttt tttgttgtac
     7561 ctgtatagat attgttaagc ctttgcaagt aaggtagtta tgtgttcaac tacttattaa
     7621 aatgtgttat ttattaaaga tacttgtctc acttactgaa ctggccgatg atcgtttctg
     7681 ccataatagt tctcgacagc cgttggatgc cctaataaat ccccaactgt cgtgatgcga
     7741 tacccttgct ctttcagata atcgatcact tgaggaacgg cacgaatcgt ttcaggatag
     7801 atatcatgga ataaaataat tgccccatcg tatactgttg cttggatctt ctgcaaaatc
     7861 aaatcaggat ttcttgtctt ccaatcttct gaatcaacag accattcgat gatcggtcgc
     7921 tggatgatcg ttgctcctac attcgtgaca gaaccatagg gcggacgaac aaaagtgggg
     7981 gttttgccag tgactttttt gatctcagtg tctgctttcg tcacttcctc cgtcatcgac
     8041 tgggcagaaa gagtggccag attctgatga tcgtatgtgt gattgccaat ctcgtgccct
     8101 gcttcagctg tctgcttcac gatttcagga tgctgcgccg tattttctcc taagacgaaa
     8161 aaagtggcat gcacgttttt ttcttttaag attcgcagaa gctgcggtgt tgtggtcgct
     8221 cctggtccat catcaaagct gattgcgacg cttttgacag ccggatctcg gctcgttttt
     8281 gtactgtctt gtgttgattg tgatgactga ctttgtacct tttccgctga ttcttgaatg
     8341 gcttctttga cgttagctgt ttcagttgat tggcttttaa tggttgattg ttctgattgt
     8401 ttgaaagatt gtgacgaatg ctccgaagag tgtgtttgtc cttttacttc ggtgatcaag
     8461 gaggccaccg aagtaatttc gaaaaacaaa agcacggata aagcaataat actgataact
     8521 aaataatttc ttttcactat atctcttctt tctctttttc tcttcttatt atttacttgt
     8581 acttacttga aaattgaaac tgctagtcgc aactacttct cgtctaatga tccaaataat
     8641 cttccgatgc caaaatcgtt tttgtcacgg acttaggaat gacaaattct ggtgtaccca
     8701 tataaccagg agcaatttcg tattgagaaa aagtcaaaac taattgatgc tctttgttca
     8761 aataaaaact tcgtgtaggc gtaacaagtg ttggtttttc tatcgtccct tcttgaatat
     8821 cttgctcagt ccaatagtac ttagatggat cttccttgac ttgttcggcc atctgccgtt
     8881 tgacttcttt tgtcaaaaca gatagatagg tgtcatcttt gaataaaagt ggaagtgaga
     8941 gctggacact gtttttcttg tcgattgtgt cgtatctttt ttcaacatgg ctatctgctg
     9001 ctgtttgggt gatctgcctt tcaatcacga gaaaccggtt atcgtctacg attttctgat
     9061 aatctcccaa aacttggtaa tgatccgttt caaaatttcc atattctttt tcagcttttt
     9121 caaaatctgc ttgtccttcg cgaaagtatt tttgatttaa tccatgaatt gttttatttt
     9181 ctttctcatt ttgtgtattg atcttcggta cctgaatatc gattttttca gatcgatcag
     9241 taaagcgttc gcctaagatc aattcaatga ctgatcctaa gatgggtaaa tcttctgcaa
     9301 agcttcgaac ttgactatta aaaagaaggc tacttgttgt aatcattacg atagccaaag
     9361 atactccgaa cccttggaga cgtctctttc tccgctttgc ttttataaat gattgctctt
     9421 cgataataaa acgctggcgg atctcctctt taagcccttt gggaattgct tgttctttat
     9481 aagttttctt tagttttttt aaattttctt tttgggaata catggcactc ttctctcctt
     9541 cattctagga tttgacgtaa agcattcaat gagcggtaca ttttcgtttt aactgtgttt
     9601 tcattcatat ccaagatagt cgcaatttct ttcaaacgaa atccttcaaa aaagtagagg
     9661 atcagaatct ctttttccgg actgtctatt ttatctaaaa tcatatggag ctctgcaacg
     9721 gaagtttcta aaggtaactg ttgggggata tcttgaattt caaataagtc cacaggcgtc
     9781 cttttttgtc tgcgccaact atctaatgcc gtgcggatca atatccgata aaaccatgca
     9841 ctaaaatgct cgatctcttt ttctttttca aaatagttca gggctttatg aaaactatct
     9901 tgcatcacat ctaaggcctc ttctttatca tgtacgtaat tcatcgtgat acggaacatt
     9961 ttttgataat cagtttcgat gagtccattc agcaggtgac ggtatttttc gatctgccgt
    10021 tttttcttat atccagtaaa aatcattctc cactcctttc tgcctcactt aatatgacgt
    10081 acaaggtgcg ctaaaagttt caattttttt attttttatg tatttacttg ggatttttat
    10141 atacaaaaaa gaaggtgtga caaaaatcgt gtcacacctc cttattctga taggctgatc
    10201 gtctctgtca caacctctcg taagaaaata atgtttattc tttttcagag tcagaaaatt
    10261 cttcctcttc ataatcttca tcatcatcgt cttcgtctat gatacttaaa tcttcttcga
    10321 tatttcctgg caagtcttct tcatcatctg tcgggtcatc tgctccgatt tcttgcaagt
    10381 cagaattata agcagcgatt tcttcatctt catcatcatc gtcatcaaac agatcatcgt
    10441 catcttcttc tgtcagatct gtatcttctg ggtcatcgtc attgtaatcg atcgcgtctt
    10501 catcatcatt gataaaggcg ttgactttct tgcgtttacg gcgacgtgga gtatcttcat
    10561 cctcttcgtc cattccatgg tttacttctt catcgatcga atcgatagca taccatgaac
    10621 gcaagcccca acgattatcc cctaatgaga taaagctgcc gtcaatattc aagtcagtat
    10681 aaaattgtgc gagtgaatca cgaatttcac tgtctgattt cccaagatag ttttggatct
    10741 ggttagctaa atcagaaaaa tccatgacat cgccacgttg ttctaagatc gcgtgtgcta
    10801 cttcgatcat tgataattcg cttttgtttg caccttcaaa tacactaatt tccaatcagg
    10861 acacgtcctt tcacagtcta ctccttatca tacaaaatca gacacaaaaa atcaaacttt
    10921 tcttttatat ttacgctgta aaatccaagg tcaaataata ttcgccgatt ttttccggtc
    10981 ccatgaacag atcataatcg attcgaacct tccctcctgt gggacggtct ttcaaactaa
    11041 catgcagttc tttcgtaaaa gtagcaatct ggatcatacc gtatggtgtt cgataggatg
    11101 tttctaaccg ttctttatag ccaaatttca aacgcatccg catttctcct gcacggatca
    11161 gctgtacatg accatctgga actaatttga tcgttacagg aatctcatcg cccgtctcct
    11221 cctgaatctc tttataacgt atgtacagcg tatcgccaat tgtcaccatt tgccctttca
    11281 aatcaaaata aaaatcttgc acttcccctt gctgttttac ttttgtacgc aatttgattg
    11341 aaatcggtgt tccttgtgat aattccatta ttcattctcc tttacttctc atagctacat
    11401 tgttcctagt ttcttttgtt ttgtcaacca acttgtccat ttttacagaa aacctttttt
    11461 ccaaaacaac tctttgattt tagagtataa tattgtagaa aatatgaatt tatacaaagg
    11521 agccggttga atgaaccctg cgattcgact agatcagcac atttatcaaa aaaacgactt
    11581 ttcccctttt gcattaacag atatattaac ggaatattct aaagaacacc agcaaatctt
    11641 tttacatttc tggcaatatg actgcacagt catcttaggt atgaaggatg ttcgaactcc
    11701 ttttctaagt gacgggatcc aaacactgaa agatgcaggt tacgctccag tgatcagaaa
    11761 ttcaggtgga ttagggatcg tatcagatga aggaattttg aatatttctt tgattttccc
    11821 ccaaagtgac acaaaaaaaa taaccataga tgacgcttac gagcaaatgc tcctattgac
    11881 taaatacgct tttccaaagg caccgataca agcattcgaa atcacccatt cttattgtcc
    11941 tggaacttat gatttaagca tccatggcaa aaaatttgcc ggtatcgctc agcgtcggat
    12001 caaaaatggg atttctgtta tgatgtactt aagtgtcaac ggtaatcagg aaaaacgcgg
    12061 gcaagtcatg aagcgatttt atcaatctgc tttaaaagaa gactttggta caaatggcta
    12121 tcctgcagtg gatcctgcta caatggcaac acttgaagaa ctgcttgacc aacccctttt
    12181 tgtaaaagaa gtcaaagaag catttgcaaa agcttttgaa aagctgtatt ctgcaagtga
    12241 tttacaggat tctaccgact ggctaaaaaa agaaatccag ctggagcaat ggaacaacca
    12301 aatccagaga atggaacaac gtaataacat aaaggagcta cattatgaca accccttata
    12361 aatatcaaat gctgcctatg gaaaaagttt ttcgtgatcc agtccataac tatatccatg
    12421 tccagcatca agtaatctta gatttgatca attcgaaaga agtccaacgt ctacgacgca
    12481 ttaaacaatt aggcacttct tctttcactt ttcacggagc agaacatagc cgttttactc
    12541 attcattagg cgtttatgag atttcaagac gaatctgcga tatcttttct cgaaattttt
    12601 caaaagaaaa aataggcaac ggaggctggg atgatcagga acggctggtg actttatgtg
    12661 cggcactgtt gcatgatgtc ggtcatggtc cttactctca tacatttgaa catatcttcc
    12721 atacgaatca tgaagccatc actgtagaga tcatcacttc tccagaaaca gaagtgtatc
    12781 agattcttaa ccgagtagaa gaaggttttc ctgaaaaggt agctagtgtg atccaaaaaa
    12841 cttaccccaa cccacaagtt gtccaaatga tttctagtca gatcgatgca gatcgaatgg
    12901 actatttgct tcgggatgct tatttcactg gaacagaata tggaacattt gatcttaccc
    12961 gaatattacg tgtgattcgt ccttatgaag gcgggatcgc tttttccatg agtggcatgc
    13021 atgcagtgga agattatatc gttagccgat atcaaatgta tgtgcaagtg tacttccatc
    13081 ctgtttcccg tggtatggaa gtaattttgg agcatctgct tcatcgcgca cacgagttgt
    13141 atcaagaaga tgccagcagt tttgaactcc attctcaatt acttctgcca tttcttgacg
    13201 gaagctttac tttagaagaa tacctcaaac ttgatgatgg ggtcttgggc acttacttta
    13261 ctcaatggtc tgaatctgct gatcctattt taaatgattt agctaaacgt tttttgaatc
    13321 gtaaaccact gaaatctgct actttttctg gcaaccggga ttcaaaatta gtccaagaac
    13381 tcactttgtt agtagaaaag gtcggatata atcctgttta ttacactgct gtgaattcta
    13441 gctacgatct accttatgat ttttatcgcc cagaacaagg cagacatcgc acacaaatcg
    13501 agattttgcg aaatgacgga actttgatag aattatcaca ggttagtcag cttgttgctg
    13561 cattagcggg acaagaacaa ggagatgagc gatttttctt tccaaaagaa atggttgatc
    13621 cgtccttgcg tgatcattat gatttatttg acgaaactta tcaagaattt gccagccata
    13681 tccgtaatgg cgcattgatt gaaataaatt aaaatatatt ggaggaaatt atgtctatca
    13741 aactagtcgc tatcgacatc gatggtactt tattaaatga gaaaagagaa gtaacaaaag
    13801 aagtaaaaga agcgattgcc gctgctgttg caaaaggtgt ttcgatcgtt ttatgcactg
    13861 gccgtccctt accaggtgtc caggaacagt taaacgagtt gaaccttttt caagataatg
    13921 attacgttat tacttataac ggggcactcg tacaacaaac gaaatcaggt aaaatcatcg
    13981 cccgtcacgg actgacacat gaagatttcc tagagatcga agtgatggca cgccgtgtcg
    14041 gcagtcatct ccattcgatc gatgacaaag cgatctacac gcctaatcga gatatcagcg
    14101 cttatacgat ccacgaagca agtttagtca agatgccgct aaaatatcga acacctgaag
    14161 aaatgacacc agacatgaac atcgtcaaaa tgatgatgat cgacgaacca gaaattttag
    14221 atgcagcaat tgcgcgttta ccgcaaactt tccgtgataa atacacaacg gtcaaaagtg
    14281 cgccctatta cttcgaagta ctaaataaag aagcaagtaa aggtgcggca gttgcgaatc
    14341 tcgcacagca ccttggtatt gatcaagatg aaatcatggc tatcggagat aacgagaacg
    14401 acttatccat gattgaatat gctggtctag gagtagcaat gggaaatgcc gtttctcttg
    14461 tcaaagaagc agcaaatgtc atcacttcat caaacgacga gcacggtgta gctgaagcaa
    14521 tcaaaaaata tgtactgtaa taaataaaaa aactgagaga aagctgatct actcgctttc
    14581 tttcagtttt tatttttttc gatccatctc tcgactgccc atcggtggct accacgtttc
    14641 aatttcacaa gcgcttcttg agggctcacc caccaaattt tattggtctt ttcagttggt
    14701 tctcctatac gcttccaaga atcagcaaca aagaaatatc ctggattata ataataagtt
    14761 gcacgataat tcgagtaaaa atactcatca gcttctccca gataagaaga aatttctgcc
    14821 acaattccca tttcttctat cagctcacgg acaatcgctt cttctttcgt ctcttctcct
    14881 tcaatttccc cgccaggaag aaaaaaagca ccatttggag cctcgattac tgcaatctgc
    14941 tgatctcttt ctatgacaat atatgctgca taacgggttt gataatcagc aaattgcttt
    15001 ttttcaccaa aaattggttt atccatgaat agtttccacc ttaacagttt tattttctat
    15061 ccattttaca tgagaaattt ctcatgaaca agcacaaatc aactatttcc ttacttcttt
    15121 ttgttatgat tgagtaaaag gaaaaggaga gatgaatcta tgaaaatggc tcacacatgt
    15181 gttcgagtga aagatcttga agcatcaatc gaattttatc aaaaggcatt tggttttgaa
    15241 gaaagccgcc gccgtgattt tccagaaaac aaatttacac ttgtttattt gacacttcct
    15301 ggggatgact atgaattaga gttgacttac aactatgatc atggtgcgta tgatctagga
    15361 gacggctatg gtcatatcgc tatagcagct gatgatctag aaaaattaca cgagcaacat
    15421 aaagctgccg gtcttgagat tactgactta aaaggattac ctggcactgc gccttcttac
    15481 tactttgtca ttgaccctga tggttataaa atcgaagtca tccgaggata gttagtatgg
    15541 ggcattttac tcttgattga ataaaaaaca aacaccaata attaaaatga gacgcaaact
    15601 tttcattaaa ttgaaaaagt ttgcgtcttt ttttaacaac cattgtagct ataaggttgc
    15661 cactctccac caatttattt ttaattcatt tgaaagaaat caaataaacg aggtaccttt
    15721 agaaaacaaa actgcttttc ctcctattat cacacgatct tcttttaatt cacattctag
    15781 atccccgccc cttgatgaca tttgataagc tgacaatttt gttttattca ttctttctgc
    15841 ccaataagga ataagattag aatgtgctga tccacatact ggatcttcat tgattgttaa
    15901 ttttggaaag aaggttctag aaacaaagtc tttttgcaca ctttctgctg tcacgatgac
    15961 tcctattcct aattcaaaat ttttgatagc tgtaaaatct ggctgcaatt tagccaccgt
    16021 tttttcatct tctaatacga aaaataaatc acgtgctaag taggcttctt taatcttagc
    16081 cccaatcgct tcttcatatt cagacaatat cggcactttt ttcggcataa tactaggaaa
    16141 atccatgtaa taataatctt taattcttgt cacaaataga ttaccgcttt gagtagaaaa
    16201 tttaatcgtt tcatcaggta ttttgtaata gttaaataaa acaaatgctg ttgctaatgt
    16261 cgcgtgacca caaagatcga tttctcgatc tggcgtaaac catctaagct caaattcttg
    16321 gtttttcttc actgtaaatg ctgtttcaga taaattgttt tcaatggcaa ttttttgcat
    16381 cgtaccttct ggcagccatt cttctagcac ataaaccgct gctggatttc ctttgaacac
    16441 ctcatctgca aatgcatcaa ctacgtaata atctaagttc attttaaagc ccctttctct
    16501 aatttgttat aaatatattc tgcacaacaa agatcttcaa tagctagacc aactgcgtcg
    16561 aaaatcgtcg gcctcgattc tcctttataa aatgtttttt tctcagaaac taattcgcct
    16621 aatgtaccaa caatacaaga ttcctctaaa tctccttctg ccatggctat aagcaggtcc
    16681 ccgctttctg ttaaggcagc tgagtaatca tctacataga tttgagaagt ttccatcaac
    16741 gatgtactta tctctcttgt atcaggagta aatgtgccca cagcattgat atgtgcgtct
    16801 tcccttaccc actctttttg aagaaatgcc tctttactag aggtcaacgt acaaatgatt
    16861 tccgctggac gtactgcatc ttctactgat attgcattta taaacgttat tttaggatac
    16921 tgttttttag ctttagcgat aaaagtattt cgtctttctt ctaaaatatc gtaaacaaac
    16981 actgtagtga tgggacgaat ggtagaaatc gcagtcaaat gcgacaatgc ttgttgacca
    17041 gctccaatta aagcaagtgt ttctgcatgc ggatttgcta agtaatctgt tgccagcgca
    17101 gatacagcag ctgttcgaat ccaggtgact gcatttgcgt ttatcaatgc tttgggtagt
    17161 ccgttttgag catcaaacaa gaaaatctct cctaaatgag aaggtaaatg atactgatga
    17221 ttatccggaa aagcggtaat gacttttgtg ccaaaatatc tgttctcccc caaataagca
    17281 ggcatcatcg caaacatatt tttgcctttt ccgtcaggta aaatttccac caatcgagga
    17341 ttttggctga ttttaccact ttcataatca aaaaaacatt ttttcattac ttgaatcgct
    17401 tcttcaaaac taaaagaagc tactacttcc tcaaaattaa taattttcat agacaattcc
    17461 ctcctcacaa aagattaact tctaaaatca atacaatcaa tggtaaaatt gtatggtata
    17521 caattttaag gagtggagaa aatgcctatt aattcttatg acaattaccc gttaacttgg
    17581 cgaccagaca aacaaaaatt gaagcgtcct tattatcgat cgttgattca acaattagaa
    17641 caagatattt taaagaataa attacagaaa aacactcagt taccctccca aagagagcta
    17701 gctgattatt tagatttaaa ttttactacc gttggcaagg cttataaata tggaattgag
    17761 aaaggtttgc tgtatacgaa tattggaagt ggcacattta tttctcccaa tgtctttgag
    17821 tccatcacca tctctaccaa tgaagtaacc gaacatatca ttgattttgg tctggttagt
    17881 tcttttgaag aatgtaataa attcgttgtc ccgtttattc aatcaactag taataaccca
    17941 aatcttgttg gtttgcttaa ttaccaagat cccaaaggca caaattttca attgactact
    18001 gcagcaaatt ggttaaaaac tcaaggagtc attccgtcct tagaatctct atctattgtt
    18061 tccggtgttc aaaatggatt ggctatcaca ctttctgcac ttttctctcc aggtgataga
    18121 atagcaacag accgatatac atacgctaat ttcattgaat tggctcagtt atatcattta
    18181 gagatcgttc caattgattt tgacgagcaa ggaatgcgac ctgatatatt gattcaggaa
    18241 tgtagaaaaa agaagattca tggtgtcttt cttatgcctt cttgtaataa tcctataggg
    18301 tttcaaatct ctcacaaaag acgagagcaa ttagccgaag tgattgaaaa agaaggtcta
    18361 tgggttattg aagatgatat ccattcattt ttaacaactt actatcaaca tacgatcatt
    18421 cctcctttcc aaaatttatt acctaaccaa acaatctact tagctggaat gacaaaattt
    18481 atttgttctg gattgcgagt tgcttatctt gtagttccag tacatgtcca aagcaagata
    18541 gaacaagcaa tttttaatat aaatgtgaaa acttctggat tagatgtgga agtcatcact
    18601 caaatcctcc attcaaatga agcaaatagg atcttagaaa ctaaattaag tcttacaaaa
    18661 agagccaatc agctttttga ttctcttttc aaagtagaaa aaccaacgaa tcgtgtccct
    18721 tactatcgaa ttcttccagt tgatcctgag attgacccta agataattga gaatgacttt
    18781 cttgctaacg gtatccgact ttatcattcc agacgcttta ctacacgtgc gcaaccagat
    18841 gcattcgttc gaatttccct atcctctaat aaccttgaaa ttttagcttc tggactaagt
    18901 atcgtaaaag aaaaaataaa aaaatatgaa ttataaccta ttgataaata aaggaatcaa
    18961 atcttattga ttcctttcaa taaaaaagag gcactacccg aggttcaaca acgggtagtg
    19021 cctcaaaaaa taaacgaaca ttttgcactt aaagtatcca agaagcaaga aaaattattt
    19081 tattttctta ttttactaaa tcactaggtt tctgtcagaa gatgaaatta tccaatcatg
    19141 acactttctt ctggatattt gtattttgat tgtgttcttt gtgcttttgt catcagagag
    19201 aagaccacag tcaatacacc aactcgtcca atgtacataa gcgctataat gatcaatttc
    19261 cctattgcag tcaagtctgg cgtcaatccc atcgtaagac ctactgtacc aaacgcagag
    19321 aagacctcaa aagcaatata ttcgatgcca gaagttttag gtatcgtttc agtcaccgat
    19381 aaaatcatga tggcgactac acacaacgtc aacgtaacaa aaaataaagt aagtgcccgc
    19441 aacactgcgc cttgtcggat ggtacggcca aatgcttccg ctctcgtacg tcctttcaac
    19501 atcgaacgca tctggatcag caacacacct aacgtagttg ttttcaatcc gccggccgtc
    19561 gacccagatg ttccgccgat gtacatcaaa aacatcgtta aaatcagacc tgcatggctc
    19621 atatggaggt aatcaactga ataatatcca gccgttctag gtgtcacact cataaaaaag
    19681 gtgtttgcca atcgttgaaa catattccct tccactagcg ttgccccatt gcgctctgtg
    19741 aaaaagaaca caacgaatcc gcctaataaa agggtacctg taacggttaa tgcgattttt
    19801 gaatgaatcg tcatcttctt tttctgatga tattccagta aatcacgcca tacgatgaag
    19861 cctaacccac cagaaatgat caaagcagat accaccatga tcaaataaac attttgctgg
    19921 tgattggcta aactgtctcc taacaaatca aaccctgcat tacaaaaact agagattgca
    19981 tggaacaaac tgtaccagat acctttcaac cagccaaatt caggcacaaa tacaaaagat
    20041 aaagcaaccg taccaatcaa ttggataaca aaggccaatt ttaggatgta aagcatgagc
    20101 ttcatcacac ctgatacttc ttctagattc agcgcttctt ttaatataat acgtgtgctg
    20161 agactgactt tcttctttat caatgcaaag catagaatcg gaaccatcat aaaaccaaga
    20221 ccgccgatct caatcaaaag catgatcaaa aattgaccag cactgttcca atgctctgct
    20281 gtattcaatg ttgtcaatcc cgttacacag acagcagaag ttgcagtgaa aagcgcatcg
    20341 ataaaatttg tcccatgccc actattacta aaaaatggta gggtaagtag gccgccgcca
    20401 actaaaatca ggagaaaaaa tcctaatgcg agaagctgaa cagccggcaa acgtttcttt
    20461 atttgtttgc tttgtttaat catcgttttc ctcttttaaa aaaagatacc acttagtcag
    20521 tggtacccat attttttact ttgatcaacc ttgttacttc tgcgcgctcg ttttcttcca
    20581 gcttcatacg aatataatag atcgtttctt ctaattgagg gatcgtcata tattctaatg
    20641 cattgactcg ccgacgtgtc ttttcgattt cactcgccat cagttgacaa gttttttcga
    20701 tttctgtcag ttccaaaagt tttggaagca gggtagtaaa ccgatcgatt gaccgatcca
    20761 gttcggcact ggaattcaaa tacccgtatt ccaaaggtgt gtctgtcagc tgttcatcat
    20821 attcaaaatt catcacaggt acttttacac tcatgatatt tttgtctacg atagacaaat
    20881 caacgttttc cgccggcaaa acaaataatt cttcgatgaa aggttccgtg atcgaagcat
    20941 ttgccaatac aaaatcagac attgcttctg tcatagactc ttccatttct tgacgcagct
    21001 ggttgttctt tcgaatcaac aggatgaatt gacgcatcag ttcatcttgc ttatctttta
    21061 ataatttatg ccctcttgtg gcagttgtca actgtttttt caatcgagtc aattccatgc
    21121 gggtcggatt cacattcaat cgtgccatca gaactcactt cccttctggc agatatttgt
    21181 ccagcatgtc gtctttgatt cgttttaatt cggttcttgg caacatcgat agcagttccc
    21241 accctagatc aagtgtttca gtaatcgaac ggttcgtgta gaatccttga ttgacatatt
    21301 ctttttcaaa ccgctcagca aacttcgcat agatcttgtc tacatcagat aaagcagatt
    21361 cacctaaaac tacggctaat tcttttgcct gttttccttg tgcataggct gcaaaaagct
    21421 gattcatcgt agctgcatgg tcttctcttg tctttccggg tccagttccc ttatctttta
    21481 accgcgaaag agacgggagt acatcaattg gtggctgaat cccactcttg taaagctctc
    21541 tagtcaagat gatctgtcct tctgtaatat aaccagtcaa gtcagggatt ggatgagttt
    21601 tatcgtcttc tggcattgtt agaatcggaa tttgagtaac cgatcctttt agtccgcgga
    21661 ttcttcctgc tcgttcgaat aaagtagcta agttcgtgta aagataccca ggatatcctc
    21721 ttcttcctgg aacttctcgc cgtgctgcag aaatctctcg caatgcctct gcatagttgg
    21781 tcatgtctgt catgataacc aaaacatgca ttcccttttc ataagcaaga tattctgcag
    21841 cagtcaaagc catacgagga gtagcgatac gttcgattgc agggtcgttt gctaaattca
    21901 taaacatgac agaacggtcg attgcgcccg tttgacgaaa atcttccatg aaaaattcag
    21961 cttcttcaaa agtgattcca atagcagcaa agactacggc gaaatcatct gatgaatcga
    22021 gtaccgttgc ttgtcgcgcg atctgagcag ctaattcttt atgaggaaga ccagagccag
    22081 aaaatactgg taatttttgc ccacgaacta atgtattcaa atgatcgatt gcagagatac
    22141 ctgtttggat aaactcatct ggataatccc gagctactgg attgattact tctccgttaa
    22201 tatccaaatg cttttcaggc aggatctctg gtccgttatc tttcggtcgt cccaaaccat
    22261 cgaatactcg accgatcatg tcttccgata cacctagttc taaaggatgg cctaaaaatc
    22321 gtacagaaga gtcacgtaaa ttgataccac ttgttccttc gaatatttgg accattgctt
    22381 tatcttcttg gacttccaat acttgtccac gacgaatctc gccattttgc atacggactt
    22441 cgattaattc ttcatatttg acaccagcaa ctttttcaac cgccatcaat ggaccgacga
    22501 cttcaccgat cgtacgatat tctttaatca ttgctcatgc ctccttctga aacgatcgtt
    22561 tgcatcgttt gcttgatttc accaataatt tgatctagtt tttctaattc tgtttccggg
    22621 atatatttgc ttcgtccgat cctttcacga atcgatacgg taccgtccat gatctcactt
    22681 aaataagcac ctaacgacaa cgcacggcgt ccttcttcac caaaggtcaa gatcatcttc
    22741 aacattttga attgtttctc tcttgaagtg aatgtatcga catcatcaaa agcattttgc
    22801 tgcaaataat cttcacgaat ggattttgcc acttctaatg tcagacggtc attatctgaa
    22861 agggaatcaa tccctaccaa acgcacgatt tcattcagct gctgttcttc ctgcaaaata
    22921 cgcatgccct cggtgaccat ggatgcccag tcagcttgaa ggatatgatc catatacttc
    22981 cctacttctg ttgaataaag agaatagctt tgtatccaat cgatcgaagg aaaatgtctt
    23041 ttttgggcta aactggaatc aagaccccag aatactttta cgacccgcaa tgtattttgt
    23101 gtgactggtt ccgaaatatc ccctccagac ggggatacag cactgattgc ggttatactt
    23161 ccttcgcgac cttctgatcc taatgcaatg actcttccag atcgttcata gtattccgca
    23221 agccgtgaac caagataagc tggataacct tcatcaccag gcatttcttc caaacgtccg
    23281 ctcatctcac gaagtgcttc tgcccatcgt gaagtggagt ctgccatgat agcaacgtca
    23341 taacccatgt cacggaaata ttccgcaatt gtgattcccg tatagataga agcttctcga
    23401 gctgctactg gcatgtttga tgtattagca atcaataccg tacgttccat caacgattct
    23461 cctgtattag ggtcaatcaa ttcagggaat tcatttaaaa catccgtcat ctcatttcca
    23521 cgctcgccac atcccacata gacaaccatg tccacatcgg cccattttgc aatttgatgc
    23581 tgaacgactg ttttccctgc accaaaagga ccaggtacag ctgctgctcc tccttttgtc
    23641 actgggaaga atgtatcaat cactcgctgt ccagtgatca tgggtgcatc cgggtttaat
    23701 ttttctttga ttggccgact acgtcttact ggccattttt gaagcatcgt caaatttttg
    23761 tcaccatttt cggtttcaat gacacatact gtttcatcaa tagtgaatga acctttttcg
    23821 attgttttga tcgttccttt tatcccattt ggcaccatga ttttatgttt tataactttt
    23881 gtttcttcta cgatcccgat aacatctcca gcactcaccg tttctccggc ttctactgtt
    23941 ggttcgaacc accatttttt ctcatggtcc aatgcgggca attgaactcc gcgccctaag
    24001 aaattacttt gtgtgatttc cataaaggta tctaatggcc gttggattcc gtcaaacatt
    24061 tgtgaaatga tccctggtcc taattcaaca gataaagctt caccagtaga gcgaaccggt
    24121 tcgcctggac caattccaga agtctcttca tatacttgaa tagacgccac atcgccacac
    24181 atctcgataa tttctccgat aactcctaga tctcccacta gacacatgtc ttggatactg
    24241 gcatcagaca tgttctcagc cattacaaga ggtccggaga cttttatgat ttttccaatt
    24301 tgcaaaattt ctatctcctt ttcatactat aagatatttt gacctactgc tttttctacg
    24361 tttttttgaa tttcttccag ccctatccct aatgatcctt gatgattggg aatcaatacg
    24421 atagcaggta tcatttgttc cttgtaacgt tcgatcgttt ctgggctcaa tgctgcacat
    24481 tgctctgtga cataaatcac gccatactca ttctttgcca tttcttcaat tgcctgacga
    24541 acggtttgct tggttgtacc ataacgaaca tcaaagccaa acaatttgaa tggcaaaaca
    24601 gaatctttgt cgccaactac accgatctta tgcgtcatag acttgtctca cccgctttct
    24661 gattgtttct ggttcgaaat ggcttctttt gccgataacc agtaaacgaa ggtttttgct
    24721 ttctacctct tttgcgttca aaaaggctag tagtggcagt ggtccgaaag cctgggtttg
    24781 tgcttcttga tacaatgaac ttagataatc atctttcaac tgttcaagct ttacgagatc
    24841 aagatttccc tcacggatga cagggcgcag aaaatcagca taatctgtct ctaatagaaa
    24901 attcgtgtaa tcttccatat ctttatctac aaagttcaaa aatacttctt tttcgatact
    24961 tcccgagctg gaaagtaccg tcgtcatgaa cccatatgtt cttttctgca atattcctcg
    25021 agccatcgtt gtgatattgg tcaagtcaat aaaagcaatg acttctgtca gcaattcttg
    25081 atatcctagc ttatcggcaa gccgtcgctg ctctgtcaaa aactgtcgat cataaatcac
    25141 gtcaattccc tgaagaatgg tagactcttc aaaataatca cgtacttctc gtatactgtc
    25201 cataatgctt tcagggagtt catttgaaac ttgcgtatga atggcatcct tcaaggtttc
    25261 taatgaataa aatccatcgt cgatatacaa atggtccaga ttttgatttg tcatctcggc
    25321 ttttgtcaaa accttcagat tatggaaagt atagcgcatc gtatagatcc aaacgatttc
    25381 aggctccggt gctgattcct ttaaccagcg aaatagacgg ctctgctcct ttaaaagatt
    25441 ctgttcgaag tcatattcat atccgtcata aatatatgga ctgtaaatcg tggatttcaa
    25501 tatttcacca agtgcttcga cagaatttgt ttgaatcatc cgttcaaatg tctcattgga
    25561 caaaagttca agctctcttc cgcgaatcaa gggatttagt tcgtggtatt ccatacactg
    25621 atccctcctt attcgaataa ttgattggca atctcaaaac tcatcgttcc ttggatatct
    25681 tgtacaaggc tgctgaaaag aaaattatac tgaactcctt gatcgtttat cagaaaaccc
    25741 gcttgatcag caacagtttc atcacttaaa accatcataa agggaagttc attgttccat
    25801 tcatccagca atgtctgaga aagataagct gctgattttt ctccagcaat gaatgttact
    25861 ttgcctgtca aaggtaatga atacaatgaa tttttgataa actggatttg ttcagattca
    25921 tcccaattct ccatttcagc tattgcatct gcaaacaatc gtcgtaaaaa ttcctgcttc
    25981 gcattcaaat tttcttgtcg tacttctact tgttggcgat tcctaagctg acggtatttt
    26041 ttttcaagta attcgatttg tttttctttt tgtttttgat gttctgtttc gatttgcgta
    26101 cgtttttgtt caaagttttg atcgatcttc attcgctctt cttgctctaa cgccgctctt
    26161 tcttgctctg ctgcttcatt catctgagag atgatttttt caatggcgtt catctgcttt
    26221 tcctccttta tgcttaggca ttcaatacta ataggaagga aataacgaaa cctaaaatcg
    26281 cat
//
LOCUS       DACIRY010000031        25789 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.031, whole genome shotgun sequence.
ACCESSION   DACIRY010000031 DACIRY010000000
VERSION     DACIRY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.031"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..723)
                     /gene="queA"
                     /locus_tag="IYE22_001104"
     CDS             complement(<1..723)
                     /gene="queA"
                     /locus_tag="IYE22_001104"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ9506326.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLN"
     gene            916..2091
                     /locus_tag="IYE22_001105"
     CDS             916..2091
                     /locus_tag="IYE22_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ9506327.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2095..2736
                     /locus_tag="IYE22_001106"
     CDS             2095..2736
                     /locus_tag="IYE22_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506328.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            2733..3650
                     /locus_tag="IYE22_001107"
     CDS             2733..3650
                     /locus_tag="IYE22_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9506329.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            3647..4318
                     /locus_tag="IYE22_001108"
     CDS             3647..4318
                     /locus_tag="IYE22_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506330.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4859..5347)
                     /locus_tag="IYE22_001109"
     CDS             complement(4859..5347)
                     /locus_tag="IYE22_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ9506331.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5548..5814)
                     /locus_tag="IYE22_001110"
     CDS             complement(5548..5814)
                     /locus_tag="IYE22_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ9506332.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(5814..6077)
                     /locus_tag="IYE22_001111"
     CDS             complement(5814..6077)
                     /locus_tag="IYE22_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ9506333.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(6070..6342)
                     /locus_tag="IYE22_001112"
     CDS             complement(6070..6342)
                     /locus_tag="IYE22_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506334.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            6553..6738
                     /locus_tag="IYE22_001113"
     CDS             6553..6738
                     /locus_tag="IYE22_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9506335.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(6880..8703)
                     /locus_tag="IYE22_001114"
     CDS             complement(6880..8703)
                     /locus_tag="IYE22_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ9506336.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9301..12006)
                     /locus_tag="IYE22_001115"
     CDS             complement(9301..12006)
                     /locus_tag="IYE22_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ9506337.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12312..13259)
                     /locus_tag="IYE22_001116"
     CDS             complement(12312..13259)
                     /locus_tag="IYE22_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ9506338.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13448..13717
                     /gene="rpsN"
                     /locus_tag="IYE22_001117"
     CDS             13448..13717
                     /gene="rpsN"
                     /locus_tag="IYE22_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ9506339.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(13893..14255)
                     /locus_tag="IYE22_001118"
     CDS             complement(13893..14255)
                     /locus_tag="IYE22_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ9506340.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14266..15387)
                     /locus_tag="IYE22_001119"
     CDS             complement(14266..15387)
                     /locus_tag="IYE22_001119"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ9506341.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15403..15753)
                     /locus_tag="IYE22_001120"
     CDS             complement(15403..15753)
                     /locus_tag="IYE22_001120"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ9506342.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(15883..17394)
                     /locus_tag="IYE22_001121"
     CDS             complement(15883..17394)
                     /locus_tag="IYE22_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ9506343.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(17716..19068)
                     /locus_tag="IYE22_001122"
     CDS             complement(17716..19068)
                     /locus_tag="IYE22_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ9506344.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19221..20297)
                     /locus_tag="IYE22_001123"
     CDS             complement(19221..20297)
                     /locus_tag="IYE22_001123"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ9506345.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20493..22238
                     /locus_tag="IYE22_001124"
     CDS             20493..22238
                     /locus_tag="IYE22_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ9506346.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22518..22955)
                     /locus_tag="IYE22_001125"
     CDS             complement(22518..22955)
                     /locus_tag="IYE22_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ9506347.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(22939..24312)
                     /locus_tag="IYE22_001126"
     CDS             complement(22939..24312)
                     /locus_tag="IYE22_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506348.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(24634..25122)
                     /locus_tag="IYE22_001127"
     CDS             complement(24634..25122)
                     /locus_tag="IYE22_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9506349.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25144..25746)
                     /locus_tag="IYE22_001128"
     CDS             complement(25144..25746)
                     /locus_tag="IYE22_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ9506350.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7395 a         5477 c         4781 g         8136 t
ORIGIN      
        1 gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc
       61 ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata
      121 gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc
      181 agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg
      241 gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat
      301 ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc
      361 tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg
      421 tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac
      481 ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt
      541 attattcata actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt
      601 atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt
      661 taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa
      721 catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt
      781 ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta
      841 tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga
      901 aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa
      961 gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt
     1021 acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca
     1081 caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga
     1141 cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa
     1201 aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct
     1261 gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa
     1321 ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat
     1381 atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa
     1441 gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac
     1501 atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc
     1561 caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg
     1621 attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg
     1681 ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg
     1741 agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt
     1801 atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg
     1861 aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt
     1921 ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc
     1981 ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca
     2041 actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag
     2101 cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt
     2161 tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca
     2221 cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct
     2281 tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt
     2341 atcgcactct ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa
     2401 aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc
     2461 attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct
     2521 gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc
     2581 gatttgattt ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc
     2641 cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg
     2701 acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg
     2761 ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag
     2821 atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc
     2881 tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg
     2941 ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc
     3001 gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa
     3061 aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt
     3121 cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc
     3181 atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata
     3241 cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct
     3301 ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa
     3361 aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca
     3421 tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca
     3481 agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca
     3541 gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag
     3601 tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga agcaattgga
     3661 taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt
     3721 tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt
     3781 cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg
     3841 tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg
     3901 attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat
     3961 catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa
     4021 aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc
     4081 tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg
     4141 tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga
     4201 gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga
     4261 ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg
     4321 ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc
     4381 tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct
     4441 tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc
     4501 tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc
     4561 atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga
     4621 attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt tccatcagtc
     4681 ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact
     4741 tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct
     4801 agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt
     4861 atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac
     4921 ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat
     4981 cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt
     5041 taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg
     5101 ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca
     5161 acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca
     5221 tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat
     5281 aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc
     5341 ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca
     5401 aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc
     5461 caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac
     5521 tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc
     5581 tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg
     5641 tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg
     5701 atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat
     5761 ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga
     5821 gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt
     5881 actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct
     5941 tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta
     6001 tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc
     6061 atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt
     6121 aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac
     6181 atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt
     6241 acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt
     6301 cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag
     6361 tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta
     6421 ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt
     6481 ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat
     6541 caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag
     6601 attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat
     6661 ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc
     6721 aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta
     6781 atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat
     6841 taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct
     6901 ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt
     6961 ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt
     7021 agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg
     7081 attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt
     7141 tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat
     7201 cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt
     7261 cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt
     7321 ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa
     7381 cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa
     7441 aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa
     7501 ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg
     7561 aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa
     7621 cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc
     7681 cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac
     7741 tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg
     7801 aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt
     7861 gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa
     7921 gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt
     7981 cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga
     8041 agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc
     8101 agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa
     8161 cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag
     8221 cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg
     8281 accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc
     8341 tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc
     8401 aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg
     8461 acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga
     8521 ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata
     8581 tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt
     8641 gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc
     8701 caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag
     8761 taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa
     8821 ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct
     8881 aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag
     8941 aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt
     9001 tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg
     9061 gtgtttaaaa gcttgcatct gcggtaataa gctcaaccaa attaaaaaca taagaagctg
     9121 tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac
     9181 acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac
     9241 tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt
     9301 ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat
     9361 gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca
     9421 gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac
     9481 cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg agacttggat
     9541 cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt
     9601 accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat
     9661 caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat
     9721 attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg
     9781 agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc
     9841 tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa
     9901 cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac
     9961 aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc
    10021 gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc
    10081 tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac
    10141 ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg
    10201 ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc
    10261 attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt
    10321 cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt
    10381 ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa
    10441 gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg
    10501 gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc
    10561 attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc
    10621 ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg
    10681 ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg
    10741 caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc
    10801 aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc
    10861 ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat
    10921 tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga
    10981 ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc
    11041 tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc
    11101 ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc
    11161 ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt
    11221 caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg
    11281 gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact
    11341 ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga
    11401 taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat
    11461 ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg
    11521 cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc
    11581 atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc
    11641 ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc
    11701 ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga
    11761 ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc
    11821 actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata atgctttgat
    11881 gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat
    11941 cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga
    12001 ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac
    12061 ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct
    12121 cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga
    12181 aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa
    12241 agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat
    12301 tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca
    12361 actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa
    12421 acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga
    12481 tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga
    12541 tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt
    12601 tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc
    12661 agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt
    12721 tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg
    12781 atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg
    12841 atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct
    12901 gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact
    12961 tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt
    13021 gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg
    13081 aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc
    13141 acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca
    13201 tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg
    13261 gtcaccatct ccctttttct ttaagtttac gtttatgtct ttattttgac aaaaaaatgt
    13321 cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta
    13381 aattatttat ctaaaagcgt aaagattacg atttacgcta ataaaaaata attggaggaa
    13441 tttgttgatg gctaaaaaat caaagattgc caaagcaaaa aaacagcaag aaacgattga
    13501 acgctatgct gctttacgcc aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa
    13561 acttccttta gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg atggacgccc
    13621 tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa
    13681 aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa
    13741 ggaggagggc gcatggacca ctccttggat catcaaaaaa gatacgttcg cttcttctag
    13801 cagaaatgct ggtactagaa gcagacgtat ctttttttac gcacagctgg tcctttttta
    13861 tatgtaaagg ctgcatggtt acttcgctat atttatttag taacaggagt cagtgcttcg
    13921 atacccacac taattttcaa tgctcggtca acttctgcca taatgggtcg atccaaatgg
    13981 cagacctttt ctttcaatct tgatttatcg atcgttctga tctgctccaa caggataacg
    14041 gagtctcttt ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt
    14101 ttagccattt ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga
    14161 ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac gactggagaa
    14221 agatcagcaa aataaatatc tcctcttttt accataggat cctccttatt cttgatattc
    14281 tcttggtatc cgctgtccta atccgcaagc cacttcatag tggatcgtac ctaattgatc
    14341 tgctatatcc tgcatagtga tttctttgtc tgcatttttg ccgataagcg tcacttttgt
    14401 tccgacagga aattcttgag gcaatcggat catcagttga tccatacata cacgcccaat
    14461 cgtttcacag tagttccctt ctaccaatag agaaaaaccc tgcatctttc gtggccagcc
    14521 atctgcataa ccgatcggta tcgttccgat ccactctgct tcaggagtga tatatgtttc
    14581 tccatagccg attccttcac ctgctgggag ttttttgact tgaatcaatt cagaaaccag
    14641 ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg tgcccagagg gattaagtcc
    14701 gtacattgct actccataac gaatcatatt gccgattgtt tcatcatgcc ataaagccgt
    14761 tgcactattg ctgacatgta catatcgagg aagttcggga agttttttca gcacttctat
    14821 aaagcgttct ttttgcagat tccaatatgt atcatctgcc tgatctgctg tagaaaaatg
    14881 ggtaaataaa ccttcccatt ctaatacacg actttctttg atgaatgcag ctgcctcttt
    14941 tacttcttca ggtgtacaaa atccgatacg acccatccca gtgtctgctt ttagatgaat
    15001 cgaaagaggc gtctctagct tacttttctc taaaacagca gctgctgctt gcaaccattc
    15061 ccgtgtacct gcagttactg ataaatcatg agcaacagct acagtcagtg cgtcgtaagg
    15121 aaccatattc aagataagga tgggctgagt aaatccagct tctctcaact cgactccttc
    15181 atctaaattt gaaacacaaa agcccgaagc tccacctgca actgctgcct cagctgtttc
    15241 aatagctcca tgaccgtatc cgttcgcttt tactactgca aacaattctt ttccttgcgg
    15301 aagtcttgca acttcatttg ctacattttc agttatagct tttttatgta tcactgcttt
    15361 cgttgggcgg tgccaagcga caaccatcgt ttttccttct ttctagtctt ccaaaataat
    15421 ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc catacattgc cttcatgagg
    15481 tgaacgcgtc acaacaggct gaccattttc attttttaaa atctcgacat cttgaaaggt
    15541 aacttttccg atcccagttc cccaagcttt agagaaagcc tctttgcaag cgtaacgccc
    15601 gcctaaaaat tctacttggc gatgaaaagg caaggactgg aatagtttca tttcatcaga
    15661 ggtcaagatt cgtgcaatga attttggttt ttcttcgatc agccgagtga tcctaggaag
    15721 ttcgactgca tcgatcccaa ttccttttat catatgcttt tctcttttca ataaatttag
    15781 aacactctca ttctaccaaa ttataaccat tttgccacaa ataagtccac aaataacaaa
    15841 aatgaaagtt tctcttaaaa aagagaaact ttcattttat gtttaatcgt tatttgaacg
    15901 gattacaaat ccgcgttttt tatcattgtg acgtttaccg ccatcttttt ggctgttatt
    15961 atttttgcta taaccgcctt ttgatttgtt tccacgataa ttaccgccgt cacgacgatt
    16021 acggttgttt ccgccaccac gtttgccatt tttattgtat ccttttttct gcattggcaa
    16081 tgggcgttct ggtgtgatct tcactggcac tgcatcagct ggatcttttg ctgtcgtttt
    16141 tagcaaaagt gcaactaaat cttgtgcaga atattcttct aataatttat ctgctgtttg
    16201 caggtattta tctagaccat tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc
    16261 taattgacct ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca tccgtttttt
    16321 cgtcaagttt tcaatcacat gcaagtagtc catttcattt ggtgtaacga aagtcacaga
    16381 cataccgcct tttccggcac ggcctgtacg tccgatacgg tgaacgtagc tttctggatc
    16441 ttgagggata tcatagttgt acacatgagt gacacctgaa atatctaatc cacgtgcagc
    16501 aacgtctgtc gctacaagga tatctaaatg accgctctta aatgaacgca atacgctcat
    16561 tcgtttttgt tgagacaaat cgccatggat tccttctgca cggtaaccgc gtgcttctaa
    16621 tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg aagacgattg ttaattcagg
    16681 tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt tcgtattctt ttgcgcggac
    16741 atagtattga tcgatcaagt cagctgtcat ttctttcgct ttgatcttca catgatgtgg
    16801 atttttcatg aatttaacgc caatattctt gatagctggc ggcatcgttg ctgagaaaag
    16861 taatgtttgt ctttgatcag gaacttgtga aatgattttt tcgatatctt ccaagaagcc
    16921 catattcaac atttcatctg cttcatccaa aactaatgtt tgaacggttc caagtttcaa
    16981 tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta cccacaacga tatgtggacg
    17041 atctttcaag ccacggatct gacggccaat atctgctccg ccgtaaacgg cttgtacacg
    17101 tacttttttg tcgcgcccta aacgatataa ttcttcttgt gtttggatag ctaattcacg
    17161 tgttggtgcg ataactaaac cttgtaattc gtgacgatct ggatcgattt tttctaacat
    17221 tggtaatcca aatgcagctg ttttccctgt acctgtctgt gcttgtccaa taacgtcttt
    17281 tccagctaat gctagtggaa tcgtttctgc ttggataggc gttgcttctt caaaaccagc
    17341 gcgttcgact gatttcaaca attctggtga taattctagt tctttaaatt tcaaatgaat
    17401 cctcctatat ggttgttaac tgaggatgta gcttttttgc taccccgtcg atttgttcgg
    17461 tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat tcttagcccc
    17521 tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg
    17581 cgttcagctt tatcattatt atgacatctg gcttcaacaa gttgtttcta tggcaaaacc
    17641 agtccaattt ctcttcatct ttcggcaata ggttattcta acatagtttt tcgttttctg
    17701 caagtttaca gatttttatt tcatttgctg aagttgttct accacttcaa ataagcccat
    17761 gctgttgctg ccttttagca taatactatc tgttgctgca agattttgtt tgatagcatc
    17821 aatcaattta ttcttttctt tttcaaaata gaacacaggt agttgggggt atttttggtt
    17881 caattcttct tttagtgcat gcatttgttc cccataaagg aaaatcatgg cgaatttctc
    17941 aggatcgata tgttcactca tttgcgcatg catatttaaa gactctttcc ctaattccag
    18001 catatctgct aacacagcga ttctgcgtcc ttttgttggc agatttttaa aagtatctaa
    18061 taccagtccc atggctgttg gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt
    18121 tcctgccgtt agccattcgg ttctgttttg agtgatagaa acatgggcga gcccttggcg
    18181 tatctcaggt atggacagtc caaagaagcg cccgatcgta tacgcaatca aagcattcgt
    18241 tacattataa cttccaggca gcgggatcgt aaatgtttct ccttctacta caaaatcggt
    18301 ttgtgctttt ccttttgctg tcacttccgc atgaacatcg ccatttccaa taccgaatgt
    18361 tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac
    18421 aacgagtaat ccgtctgata caagtccgtc agtgatttcc attttggctt tagcgattcc
    18481 ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt
    18541 tggttgagcc ataagtgaaa gttccgtgat ttcattggcg tgatccatgc ccatttccaa
    18601 aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg taaggaagac caatattatt
    18661 gttgtaattt ccttgcgtct tatatgtttt gtatttttca gacaagacag acgcggttag
    18721 atctttcgtg gtggttttac cattgcttcc tgtaattgca acgacttctg cgcctacttt
    18781 ttccaagtaa taggttgcca attgctgcat ggctttcaaa ggatctgcta caggaataac
    18841 tggaaaattt tttggagctt gttcaacagg caagctccaa aatgaagcag atgctccgtt
    18901 tgtgatcgct tgctggataa attcatgtcc atctcgcgta ccagcaagag gaacgaataa
    18961 acttccttcg gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga
    19021 gcattcgctg cccacggctt gtgcaatttc ttgtattgtt agattcattg agatctctcc
    19081 taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga ctctcacttc
    19141 caagtaaatt tctctattcg tatttagaag ctagaatgaa cgcggttacg caatcactcc
    19201 agcctcttct ttatttctct ttattcattt ttttcaaaaa aagattgacg ctgatggtat
    19261 cgattcattc ctaactggat cagttcttca atcaaatcac cgtatttcaa gcccatattt
    19321 tcccataaga gtgggtacat actgaactcc gtaaatcctg gcatagagtt taattcattc
    19381 aggaataatt catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct
    19441 aacatcgtgt aagctaactt cgcgtactct tgcgcttttt gataaacttc ttctggcact
    19501 tcggctggaa tctgcatttc gattttatta ttgatatatt ttgcttcata atcatagaat
    19561 gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca
    19621 gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga atcatactga
    19681 taggctgttg ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt aatgccgaca
    19741 ctagaaccca tattcgccgg tttgacaaac atcggataaa gcaaagaacc ttcacattga
    19801 tcaaatactt ttttaggatt ttctttccat tgattcttaa gtactggtac ataaggaact
    19861 tgcggcacac cagcagcttg taaaatatac ttggtcatga ttttatccat ggcacatgca
    19921 ctggtcaata cgcctgcgcc gacataaggc atattcaatg tctctaagaa gccttggatc
    19981 gttccatctt ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc ttcttctttg
    20041 atttcgcccg gattgatcac ttttcctgta aagccttcct ctgtctgtcc acttgggtcc
    20101 catgaaagat gcaagacatc tttgctggca ggtttttctg ttagtaatgg acctttgacc
    20161 cattgtcctt cttttgtaat aaatacgagt tgaacttggt aataattata ataaatggca
    20221 tttaaaactg aaaatgcgga aagaatggac acttcatgct ctgcgctgcg tccgccatat
    20281 agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa atcgttcagt gatttactct
    20341 catattttag cacaaaacaa aaataaacga ataacttatt tataaaaaaa cgagccagct
    20401 tctaaaagaa cgagcgtcat ttattttttg tcagctagat tatttgtccc gttttttata
    20461 aaaagttatg ataaagaaaa ggagggatga tgatggcgca aacaaatcca cgcagccgct
    20521 tggcagaaat cattcgagtg ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa
    20581 aaaatcccga acaggtaaaa aaagcctttg aagaactagg ccccaccttc atcaagatag
    20641 gacagatgct ttctgttcga gaagatttgc tttcttctgc cttcactcaa acattcaaaa
    20701 cattacaaga tagtgtacct agcgacacat tttcaacggt caaaaagacg atcgaaacag
    20761 aactttctct ttcattatca gatatattcg atgatttttc aaaatcgcct tttgcctctg
    20821 cttctatggg gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga
    20881 tccaacaccc taacatcgcc gaagagatcc gacttgatct tcaactattt gaacgagcga
    20941 tccctttgat caaatacatt cctgaaacta gcgtagttga tctaaaaggg gtactccaag
    21001 aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac
    21061 aattctatca aaagaacaat ggctggaaag agatccgcag ccccaagatt tatgacgctt
    21121 tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg aatcatttaa
    21181 tgaacgccga aaacaaaact gaaactttta tcactggaat tcaaaacaag cagctgaaac
    21241 aagaagttgc aaagctttta gttgagaact ttatgaaaca ggtgtttgat gatggttttt
    21301 tccatgctga tcctcatcca gggaatctgc tattccatgt actaacaaaa gaagaacaga
    21361 cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc
    21421 gagcctccac ttctgcagaa gaccctgttg cgccatatac catcaactac atcgattttg
    21481 gtatgatggg gcatctttct gcaggactta ggcaaaaact cacacaagcc gttttagcgc
    21541 tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag
    21601 gctcgtttga tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa cagtattatg
    21661 attctcctat tgatgaaatc aacttgcaag aagtattcac acatgtcgtg acgatttgtc
    21721 atcaaaataa tctgcagttc gatcgtgata tcacgctttt gctgaaagca tttgggacat
    21781 tagaaggggt gatccgcgtc ctcgatcctg aagtttccct catggaagtc gcttcgcctt
    21841 ttgctcaaca ttattttctc actcatctgg atgtagaaga tacattgaaa caaagcggat
    21901 tagacttatt ggaagggatg aaggctgcgc ctaagatccc tcaacagctc caccatctat
    21961 tagaaatgtg gacttcggga caaggaaaag tcaatttgga attgaaaaag caagacaaat
    22021 tattgagcag gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt ttagctgcat
    22081 tgatcgtagg gtcctccctg cttgtccaag cagcacctgt agaaaacgca gaggtcgttt
    22141 ccttattagg gatatttacg tatgctattg ccgcattcgt cattattttt ttggcaatcg
    22201 atgcattgat ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg acagaaagaa
    22261 tcagcttcaa gaaataagaa ggggttttac ataaattgtt tctcaaattt ttgtgaaaat
    22321 tcggcttatt tttcgaagag gctacttctg aaacaccgag aataaaaaat gtgaaaaagc
    22381 tgttctgcac ccagtaataa gaacaatcaa aaagggaaca tggaacacat ttatcctgtt
    22441 tccctgttcc ctttttgatg tattcgagca taaataaaat accttcgtta cctactatct
    22501 tctgtacttg gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa agacatatga
    22561 tctttctccg taaagggttt tggacccttt gtccttatgc cagcttttct taactttcca
    22621 ctatgataac gttgttctag aagcgtgccg atattcgctt cgtcaaattc ttttccagag
    22681 tgatggaaca ctcgcacacc cttcggcaat acaagtgaag ctaaaccata atcttgcgtg
    22741 atcaaaaagt cattcttctt aaccataccg acgattttat aatccgcact atcagctccg
    22801 cggtctacgt agacgatttg gacatgatta gcgtattctt tggtcgtata atggtccaca
    22861 ctggtcacga taaccacgtc aagaccatag gattctccta attttatgac atcttctttt
    22921 actggagaac catctccatc aatgaatagc ctcattcttt ttcgccttct tccataaatg
    22981 ataaacagtt actaatccgc tcagtaaaag accaacattg atccattttg ccaagataag
    23041 cagttgtaca ctttctatcg gcactgattc atggttcagc gtgatcaata atagatggat
    23101 tggctctccc atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa actggtggat
    23161 cacactccct ttattttgag tagtatactt gacaacgatc cggcgatcca ttggaatagc
    23221 tgtatcagtt tgataatatt ccttatttag ccagctgcct gttactacgt gccagaaact
    23281 aagcagaaag acacctatcg caaaaccatt tggaatcaag aggaccaata aagaagaact
    23341 cagtccaagc aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc
    23401 gactattttt gctgcaatca agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt
    23461 ttctaatccg tacagtatgc caacataaaa actgctaaat aaaaaaagga aattccctag
    23521 cattccattt agaaaagtaa acagatttag ccagcttggc aacttccatt ccttcttctg
    23581 gtgtcctaaa acaaacacga acaagatgaa cagcagcaag aaaccataaa tagcaagatc
    23641 aaaaagcctt tcatcgaata acaatcgggc atttctcaaa agaaacaata agccaaagaa
    23701 tatcaaaaat aaaacaagct ctcgcgtagc taccagattt cttttgatgt ctttgaaatc
    23761 cagttcgtaa cgcggataat gactaaccgt atggtaggcc atgacgtaaa aaagtgtata
    23821 aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag
    23881 aaatagccag atagctgcaa ataagtactt tttcccatcc atattgataa aaccttgcgt
    23941 cttttcgtga aaattgaccg tggtattagc aggtacaagc catgctgcac tcagccctag
    24001 caaaacactt gaaaaataaa ataaaggaaa ataaaatacg cctaatattc caagcaatga
    24061 accagcacca ccaatcagca gagcgatcat cagcaacgtg taactatcta gaccaaatcg
    24121 tagactgttg actaaaaata gtccagtcat ccgaaaggca taaaacagcg taaatggcaa
    24181 aatcgtcagc caattatgcg agtctgttag tcccaaaaat aaaagataag gaataaaaat
    24241 aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag
    24301 tttttcgaac atgacaatgg ctccttaaac agtcttataa ctaatctgct cttcgatttt
    24361 agcattaaag gggaaataaa ccaattttac agaatatatt cttgtaaaaa aaaatacaga
    24421 aagaagcata agcttctttc tgcattttta cggtacttga ttactcaagc agacaagccc
    24481 ttctccacaa aagtcttgcc cgacacccct gcatccggca ggtgataaag atagaaatca
    24541 attatcctgt gcctccaaaa tcaattagga gctctgtaaa ccagagtcct tctcgacccg
    24601 tcgcataaat ttactttaac acagttccta tgtttatgcg agagcttttt tcattttata
    24661 atcgaccatt tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc
    24721 ttcatgctta taaacctcga ttgcttgatg attcccctga ttcactcgca aaaagatctc
    24781 tctttccttt gtttgcgcag caagttcttc ataccagcga aaaactgaag aagtcaatcc
    24841 ttgaccacgt actttattta gcaaatataa tttacttaaa taaatttttc catttatctc
    24901 ttcataagca gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt aggacacacc
    24961 ttctgtcagt tctttttgaa tctgtttttt tgactgataa gttttcagca tgtaatccac
    25021 ttgtttttga ccaatgatcg gcacaaaagc ttccggccat acttcttgta tgactgccat
    25081 cagctcatcc agttcatttt ctgttacttt tattttttcc atacacccac gtcctttttt
    25141 acattataaa tgaccttgcg cttgatggaa taaaaagagc atgatcatgc tatacacacc
    25201 caccgaccat ggtttcaaga aaatcacaat ctgcataaat tccctaaaag ataatttcgt
    25261 caaaccagct actagacaga tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac
    25321 tagtactttt ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt tctgatactt
    25381 ttttggcgat aaaataagct ggacaaaggc tacgccatag taacggacaa gcaaaaatcc
    25441 gattgcttcc ccaatcacta ggccaataca actatataaa agcccaaatc cattgccaaa
    25501 catcagcatg cccgctactg aggagatccc tcccggcaaa ataggaatga tcacttggat
    25561 cacttgcaac agaacaaata tcatcactgc atacttgcca aattgctgga taaacgcttg
    25621 caaagacgtg ctcgatcgaa aaatcccatg atgataagca taaagaatca acacaaagaa
    25681 aaaaaacaga ccaataatgg gcatgatctg aagacatttt tgtaaaactg atcctttttt
    25741 agacatctat attccccaca tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DACIRY010000032        24617 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.032, whole genome shotgun sequence.
ACCESSION   DACIRY010000032 DACIRY010000000
VERSION     DACIRY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.032"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            66..767
                     /locus_tag="IYE22_001129"
     CDS             66..767
                     /locus_tag="IYE22_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ9506351.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IYE22_001130"
     CDS             764..1885
                     /locus_tag="IYE22_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ9506352.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IYE22_001131"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IYE22_001131"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ9506353.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IYE22_001132"
     CDS             3264..4172
                     /locus_tag="IYE22_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ9506354.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IYE22_001133"
     CDS             4207..4479
                     /locus_tag="IYE22_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ9506355.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IYE22_001134"
     CDS             4490..5536
                     /locus_tag="IYE22_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ9506356.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IYE22_001135"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IYE22_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ9506357.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IYE22_001136"
     CDS             complement(8547..9218)
                     /locus_tag="IYE22_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506358.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVNLKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IYE22_001137"
     CDS             complement(9211..9705)
                     /locus_tag="IYE22_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ9506359.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IYE22_001138"
     CDS             complement(9725..10945)
                     /locus_tag="IYE22_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9506360.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IYE22_001139"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IYE22_001139"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ9506361.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13123..14034
                     /locus_tag="IYE22_001140"
     CDS             13123..14034
                     /locus_tag="IYE22_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9506362.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVLSRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15030..15281
                     /locus_tag="IYE22_001141"
     CDS             15030..15281
                     /locus_tag="IYE22_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506363.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15364..15636
                     /locus_tag="IYE22_001142"
     CDS             15364..15636
                     /locus_tag="IYE22_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506364.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            15726..15965
                     /locus_tag="IYE22_001143"
     CDS             15726..15965
                     /locus_tag="IYE22_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506365.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            16112..20395
                     /locus_tag="IYE22_001144"
     CDS             16112..20395
                     /locus_tag="IYE22_001144"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ9506366.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            20494..20859
                     /locus_tag="IYE22_001145"
     CDS             20494..20859
                     /locus_tag="IYE22_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506367.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            20861..21313
                     /locus_tag="IYE22_001146"
     CDS             20861..21313
                     /locus_tag="IYE22_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506368.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            21927..23237
                     /locus_tag="IYE22_001147"
     CDS             21927..23237
                     /locus_tag="IYE22_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ9506369.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            23260..23571
                     /locus_tag="IYE22_001148"
     CDS             23260..23571
                     /locus_tag="IYE22_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506370.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            23932..>24617
                     /locus_tag="IYE22_001149"
     CDS             23932..>24617
                     /locus_tag="IYE22_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9506371.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGG"
BASE COUNT         8330 a         4064 c         5206 g         7017 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaatt gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggatgagc aagatagtgt gaaataactt ataatcaatc gaaaaaccta ttaaataaac
    12961 ctttttgagt agatgcaaca ctgaggatta tctgtataaa agattttaaa ctgtcatttg
    13021 gttatcacgg cgatactaaa attaacaaaa ttggaatatt tttcttgaag atttttagct
    13081 gtcagtatat gatgaaagca tctcatagga ggtgcttttt tcatggttga taattcaagt
    13141 ggaagggttc ttaaatctct aagaaaagag aaaaaactat ctcaaaaaaa gttagctgat
    13201 ttagcaggta tttcacaaag tactttagtt aaatatgaaa aagggtcaag aaaaatccct
    13261 aaagacgtag ataatactct aagcaagatt ctgaatatag aaactctaat aaaagatgag
    13321 gaagataaga ttgagattct catcggtaaa ctaattgcat acagagatat gaataagtta
    13381 cttaacaaag agttagctga taatatagga attagtgaag tattactcag ttatgtgtta
    13441 aatagaaaga gaaatccgtc aaaagaaatg caaaaaaaaa tcgatatttt tctattaagt
    13501 aatgaaaaag aaattttgaa ggagattaat cgtgatagtg agatatttag tttatcaaaa
    13561 gacgataaaa ttgttatggg aaaaagaata cgagaagtcc gaaagaaccg ggaagagacg
    13621 cttgaaaaat ttggtaaaaa ctttaccatc tatacaggca aaaatgttct aagtagatgg
    13681 gaaaaaggta ttaatattcc agatattgaa aaactaatga atattgccta tcttggtaag
    13741 gtcactgttc cttaccttat gtacggagaa gattataaga atatacttcc aaaagatgaa
    13801 agagttagcg attttaaaaa gataaattct tttagtatgg gattaaggat gaggaaaatt
    13861 aggaaagatt attatctaga aagggaggag tttggaaagc ttttttctcc ttcaatttca
    13921 aaatggtcca tagataggta tgagaatggt agagatatac ccaatactaa tcgaattatc
    13981 caatatgctt atataggaaa tctttcttta gaatttttga tttacgggat ataaaaatag
    14041 tttacgtcga tacttattaa gttatcgacg ttttttatta tagaaaaaat ttataaaagg
    14101 aggtttcttg atatgataag aaacaaaaag ggtttgttga aagttgcttt actggcagct
    14161 tttttctaag taactttttg acaagctcaa tggcaacaca agcggaagaa gtaatacaag
    14221 aactaactgt tatgaataac agctagataa aatgggctat gtatgttatc caacaattta
    14281 gtaatcacta gaagcctagc gaaagttcgc ttgataagtt atagatgagt attgatttgt
    14341 agtgtttcca aatcaaaagg gtacagagcc acatgaaata tgttgtcctc tgcagcgtca
    14401 ggatgggaat ccataccatg gagattcgga gatctgtgag ggaatactta cgtggttaat
    14461 atgtcagttg tgttggacgt agtaatggtc atagtgggta gattgactga attcttatat
    14521 gagaaaatga aaccaacaag aagatagggt tatttataca gaattgggga tcttttctgg
    14581 aaaagtaatc ggataatgcg agattcgtac ggtctttttt aaatttctct aaaaaaaacg
    14641 gcatcgaatg ggttgggtga ttgataagca atgaatcata gactgatata gaataaaaaa
    14701 tgattctatt tatcttttta catctatcgt tttttcaagt agccgcaaat tatatcagca
    14761 ttcaacaatt tttgtataca agttctagta aaaggatctg aagaaaaagt tggttgttgt
    14821 gaagataaaa aaagaatcag gttttactga gtttttcttc aaaatttgtg ttattaggtt
    14881 aggacaaaga taagaagaag tatgaagatt taaaaaagga atgaggaaaa gatagtaatt
    14941 tccgattatt ggagtttttt tatttataaa gaagaagcga ataagttgtg attcctagat
    15001 ttcgcgcgtg attaattaat gagcaaaaaa tgcggaaagt tttagtgttt aatacacgag
    15061 gacaaagttt cttaggtatt gaaggcacac gtgtaacggt tgatgggtgg tgtgattgtt
    15121 tcgaggttgg aaaacatgtt gaacttatgg catttactga gctaaaagat aagaacaacg
    15181 tggaattttt cgagagtgat attggttggg acgatcatga agaagtttat ggaaaattta
    15241 ttttgcggaa gggaaattct tatacgaatg gggaagcata agtagcgatt tgttagagat
    15301 cattgatagg attgaaattg tcgggaatat ttatgagaat ctagagttat ttgaggtgat
    15361 agaatgagtg atggttttga acaagtatca ataattaaaa atcaagttcg ggagattctt
    15421 agaaaaaaag gctatttggt agatagttat tttgaaggag attacgaaac atgggtaggt
    15481 gtctatgctc gtccagagaa taaacctacc tatttagatc ctacgacgtc agaagatgcc
    15541 tatttacaaa ataggtatcg agtagatggt tttaagcaag actttgcgga gtggtttgaa
    15601 tgggaaattg aaaacggaga agtaaaagaa gaataagagt acttgttgta tcaaaataag
    15661 ccagtagaac aaccaaacca gtagctagta atgacaataa tactgaggaa tcggatcatt
    15721 atggtatgat tgatatttct cttccagaag atcttaagcc agatcctgta acaattggca
    15781 atagtgtcat gatttttgat tcattagatg aagctatgtc atatggttgg aaagaaatta
    15841 aggatgaagg tagtaattgg tatggcatga gatttggtga tggtgaagtt gccaaaaaag
    15901 ctggtattcc aattgggaaa tggacagttg atttccacga agcaggttct ttttttgttc
    15961 tgtaattata gtgggagggt aaaatcattg atgaaatgtg ttcagataag agcgccagat
    16021 aaaaatagaa gagacagttc acgtcgttat cacttttggt aacggcgttt tgttttatta
    16081 tataaaattc tcaggaaaag gaggaattct tgtgtatgca aaaacgaaaa tgaattggtt
    16141 gaaagcactc gcattggtgg ctttttctca attctgtttc ttggtgggaa ctgctcaagc
    16201 acatgcagaa gaaacgacgg ctatacaacc tacgatggaa gccattgccc aagtcgcttc
    16261 tgaagtgaca gaagtccaac cagcagaagc cggggtccca gctggtgaag ccactgttga
    16321 acaggcccct gtgacagaag cgccaacggt caattccaaa acgactctcg atcagacgat
    16381 tggggaaaca caacacccag ggaatagtcc tggtacttca caaccagttg aagaaactgc
    16441 ctcaattaca acccctgctt cagaaacgtc cactctagaa ggagaaaaag ctgccgaagc
    16501 aacgccttct gtgcagccag agtcttctga gaaacaagcg gaagaaaaga aacgaataga
    16561 agaaacagtg gaacaaccag cggctactgc tgacaaagaa ggaaaaaatg aggaaaacgt
    16621 tgaaaaaaca atctcttcaa ccgcagctag tcaaaatcaa ccaacgaaac caaacaaaga
    16681 atctacggct agtattgaaa atcaaaagaa aaaagagcag aacattccaa aaacaggtac
    16741 acctacactg ttagtaacag taccaaccaa tcgttcggtt cgtgttcgcc gtgcaaccaa
    16801 tgcagctgtt ttaggtggtc ggaaaattga ttggggcgaa aatattggtg ttacttctag
    16861 tccgaacaat ctaaacttca ctttaaatta tatggttcgt tttgagatta atggacaagt
    16921 ggcgttttgt attgagcctg gtgtctcagc cgcagaaggt caagtttata cagctactgc
    16981 tttgaaaaac tatttgaaaa attcaaatgt acggaaaaaa acaagcttga tctcttattt
    17041 aggatatgtc acaaataaag acaaatccga tgaacaatac attgcggcac aatttatgat
    17101 ttgggaagct ttgggcacaa aaatcccaat caaaaatatc cgtgttgatt atgatcgtcg
    17161 aaaagcagaa attcaacgtt tgattaataa ctttggtgaa aaagcaactt ttaataatca
    17221 aacacatacg gtaaaaattg gcgaaacgtt gaaagtaaag aataaaaatg attttttgaa
    17281 atatgtgaaa gacattcaaa aaccaaaagg tgtcaatgta tctattcacg gcaatgaagt
    17341 atggattaca gtcacaaaag aggcaccgga taaagcaaaa attcaattga ataaaattaa
    17401 agttcaaggt gcgccattag cctatcgtcg tccaggagct caaacaattg gtgtgttaca
    17461 tccaacagaa cctaatgcgt ctgttattaa tttacaaatt ctaaaacaag gaaacattcg
    17521 tgtgtttaaa gaagatgcgg aaactggtgt gaaagcacaa ggtgcagctt ctttagaaaa
    17581 tgcggtctat ggtttgtatc aagcagatgg gaaaaagatt aaagaaatta ccttgaaaaa
    17641 aattaacggc aaagtacagg cagaaattaa agatcttgat cctggcaaat atgttttaaa
    17701 agaaatcaaa gcgccaaaag gttatgtgct ttctgatgaa gcaattgaaa ttgttgttga
    17761 acctggaaaa actgtcaatg cgaccgcaaa agatcaagtg atcaaaggaa ccattgattt
    17821 agtgaaagtt gctaataaag atttagtcga ttcaacgaat ccagataaca aaccaaaatt
    17881 agctggcatt gaaatcactt tgacgtcaaa aacaactggc aaagtggtga aaaccgttgt
    17941 gacagacaat gatggttatg ctacttttgg taaaaatacc gttgtctttg atacgtacat
    18001 tctttctgaa accaaaggaa aagaagggta taaactattt gaaccacttg aagtcacaat
    18061 ttctgaaaat gggcaaactt tccattatgt tttagaagat aaagtaattg aacaacgtct
    18121 taaagtggtg aaagtggatc aagaaacagg aaaagtgatt ccacttgcta atacagaatt
    18181 caagattttt gatactttag ccaatcgtta tgtcacaatg gagattccaa atgatacaga
    18241 aacaacggat atctttaaaa ccaatgaaaa aggctttttc ctaactaacg gtacacttac
    18301 ttatggaaaa gatcgctatc gtttggaaga aatcaaagcc cccgtaaatt acgttttgaa
    18361 tcaaaaccca ttagtgttct cgattaccaa tgatacagaa ccgatcaaag tgatccattt
    18421 tgcgaaccgt caagcacgga aaagtgtaaa attattcaag tatgaagaat ggaacaagaa
    18481 gaaaacgccg ctcgcaggtg tgaactttac cttgtataac ggtaaaaatc aagcaattgg
    18541 tgaatatacc actgatcaaa acggggaaat tttagtgaaa ggtctagttg ccgatgacta
    18601 ttacttcttg gaaaatgccc cattagaaag tttccaacca aatacagata aacaagcatt
    18661 cacggttgca ttcaaacaaa atgcgccaga tgatggggaa acgttagtgg tttctgtacg
    18721 taattacttg attccaccaa aactagaaac acaagcgtca aataaagccg atggtgaaaa
    18781 agtggttgat ccattagaaa cgatcgtcat taaagacagt gtttcttata ccaacttgtt
    18841 tgtcggaaaa gaatacacca tttcaggagt attgatgaac aaggcaacga atgcgccaat
    18901 tttacaagat ggcaaagaaa tcacaagtac cttggccttt attgcagaca aagtcaatgg
    18961 cacaaaagac cttgaatttg tggttaatgc tagtgttctg aaaggtcaaa cgatcgttgt
    19021 ttttgaaaaa cttttccgtg atggcattga agttgcagca catgcggata tcaacgatga
    19081 agcccaaagt gtgaaagtga ataatccaac cgttcatacc acggctgtct ttgaagacgg
    19141 attgacagtc gcagacccaa caggattgtt taccttaaac gataccgttg aaattaccga
    19201 gattcttcca ggaaaagcat acttggtcaa agggatctta atggataaag caaccggtga
    19261 acccttgctt gtgcatggaa atacggtaga aacgagtgtt ggctttattg ccaaagcaga
    19321 aaatgaaacg gtgatcgtac catttacctt tgatttgaca ggtataaatg gtcgtgaaat
    19381 cgtcgtgttt gaaagtcttt ttagaaatga tgaagaacta gcaaaccata aagacatcaa
    19441 cgacaaaggg caaacggtac gagtaactaa cccaagtgta cgtacaacgg ctacacataa
    19501 acccactggc ttgaaagagg ttaatccatt aacgaaagtc accattacag acgctgtgaa
    19561 ataccacgat ttggtcgttg gaaaaacata cacagtcaac ggtgttctaa tggacaaagt
    19621 aacggaaaaa ccagtgctag tagatggaaa agaagtcaca agtaccttaa cgtttgtggc
    19681 agaaacaacg gatggtacag tcaatctaga ctttattcta gacgcacgtg cgttgcgagg
    19741 caaagaagtc gtggtctttg aagatctgtt tagagaaggc gtattattgg cttctcacgc
    19801 agatattacc gacaatgacc aaaccatcaa aatcagtaat ccaaccatta cgacgaaagc
    19861 gacgaataaa aatggcggaa aagagatctt ggccttgcca aatcaaactg tgcttgaatg
    19921 ggtcaaagta gacggtcttg cgattggtca aatctatcgc ttaattgttc aaggctactt
    19981 aacaccagac ggcacaccat ttgaaggcac tcatgcggaa aaagtctttg cggccgataa
    20041 agaaatgatg gaattcttat ttgaattctt agtggacggt cgcaatcttg caggaaaagg
    20101 cttgagtttt gcggaatggc tccaagcgaa aacggaagat gagaaagaaa cgtttgaaga
    20161 agtggcgaaa cacaatgtag atctaacaaa caaagatcaa acggttcaaa ttgtggaagc
    20221 gccaaaacca ccgcaaccag tgaaaccaga acaacgggtt aaaccagcta ctgttgtacc
    20281 aatgttgcca aaaacaggtg aacaaacaag tagtatactt atggttcttg gattagtagt
    20341 attagtcatt gctttaggta taacacttta taaagtacgt aaagccaaag attagtcatg
    20401 aggaacaagc ggttaggatt tttaaccgct tgtttgattg taatgtagaa atatttagaa
    20461 tttattttgg tttttaagag gaggaaaaac tttatggcag aaaaagcatt gcgtatgaag
    20521 aaagagtttt taggtcaagt atttttattg ggttcacggg aacctaatcc accaaaggaa
    20581 attaatggtg tgtctgggcg atatgttcaa ttacaagcag agtgttcgcg agaagaacag
    20641 cctgcttttt ttgtttatct cacagctgaa gaggataaaa gattacgatt accagaaaaa
    20701 gaggtgcgta tttcatttga agaagcttat tttgtttttc gtaaagaacg agtgaatagt
    20761 cgtgaaacaa atgatatggg acaaacagag cgtcgaacaa ctggctatca agatgttggt
    20821 gtctttttaa ctggtgtaaa aattggggag gttttgtaag atgggattaa aatttccaaa
    20881 taattatgac attacagaag cattagatga aacttgttta ggagccgtac gctttggcgc
    20941 attagaagag tctgatcgtg tatttgataa tgaaacaggc gattatacgg gagaaattcg
    21001 atcacagcga gcacaattag tttttggaaa tccagatgaa ccaaagattg cacgacgagt
    21061 taaatttcca gcagatgtag atttgactgg ttttaaacga cgtgatcctg tgaaattaat
    21121 tgatccagaa tatcatgtgg tatatattcc agcacaagat ggcaatcgtg aatattcaga
    21181 tttagtgatt tatgcgaaag gattagagaa agtagaagct ccaacaaata atcctaatat
    21241 gaaacaatca caagaaaaag gtttagatca aaaacaacca agtgttgatt ctaaggaaaa
    21301 agagaaaaaa taaggttttt ttgaaagaat aggaaggagt cttcctattc tttttatcta
    21361 agatagaagg aaggggagat tttgaattca gataaaacca atcaaatact ttgagagatt
    21421 gtgcttgtta tcaaaatatc caacaattga ttgagtattt tggagacaat ccaacgattt
    21481 ttgtacaaac aaatgcatat atttgtgcgg ttattgggca gtgtatgttt ttacgtgatc
    21541 ttgttcgttt ttcattaccg taccagtgga aacaagatca tcaggaagtg atttggattc
    21601 gtatagtacg aatatgtaat atgtgtgcac acccttataa aaatcgatta aatctttttc
    21661 gattatgttt ttttactaaa tgtgatattc ctgtattagt tgctttttgt caaaagtttt
    21721 tagcacagga aaagtaaaaa cagaaaggag gaatgtgaca attgaaagct cattttttca
    21781 agtatcgtgg tcggcgaatt cgctatttca atcgttattt atggttttgc tattatggtt
    21841 tctttttgtt tccatttttg attctgttag gttggtttag ttggaaagta ttttatccac
    21901 gtttaagtac gttttcgaat tggcaaatgt atcttattcc tggaataagt attgtgttgt
    21961 atttgttgct ggttagtggg ttaattttag ggttgatgca ttggtcgcga ctaaaagaag
    22021 gatattttgc ctctgtttat tggcgtcagt tacttgttcg aatgttaatt gacaatcggt
    22081 ttgtttatac aaagaaacaa gttagtgaga aacaaacaag ggaaaaaatg agattaccaa
    22141 aagtttattt ttatcaaaag aaacaagaat tagtggtttc ttttccttta gatggtgggc
    22201 gttttcacga acgattttta aaaatgggtc atttgttatc tgaagtattt ttgcgtgatt
    22261 taattcgtga agaacgagaa aaagcccgaa tcaattatgt attcctttca gattctggtc
    22321 ggattcctat tgatcaatgt atagcagaaa atggacgtat tcatttaatg aaaactttgg
    22381 attgggtata tgatcaatca ccaaatatgt tgattgctgg tgcaatcggt ggtgggaaaa
    22441 cctatttatt gtattcttta attcaagctt gtttaagtgt tgggacagta gatatttgcg
    22501 atggaaaagc ggcagattta gcagcattag gtgacgtcga tgtattcaaa ggacatgtgt
    22561 tttacaaaat aaatgaagaa atgattattt gtttacgaaa tgcattaaaa gaaatgaatc
    22621 aacggtatgc ctatatgaaa accatgaaag atcctaaata tgaaccaggg aaaaattatg
    22681 cttattatgg gttaccaccg cattttatct tttttgatga atgggcagct ttttatggtt
    22741 cgttggatat ggaagcgaga aatagagtcg ataaattgat tcagccatta gtattaaagg
    22801 gtcgtcaagc agggatgtac attattttag caatgcaacg accagatgca gaatattttc
    22861 caagtggtgt tcgggataat ttaacattcc gtatttctgt tggtcgattg tctcccattg
    22921 gttatttaat gacttttgga gaagatttta aaaataagcc attttacaac aaagatcaac
    22981 ctggtcgtgg atatgctttt ccaggatttg atgtacctgt agagtttttt gcgccatttg
    23041 tttacaaaga ttatcacttt atcaaagaat ttgagaaatt tactgaaatg attgtctatg
    23101 attttacagt aaaagaggat agtcaagatc aaacgatggg agatgataat cagttgttac
    23161 aagaacaact agagcgccaa atgtatttag caaaagatcc aaaaggaaca agtttttctg
    23221 atgcgcatat tttgtaatca actaaggagg tgaagttaaa tggcagagag ttgttattat
    23281 agagtagcta cagcaatacg aatgattaat ccttctcttt cttccagaac gttttatgat
    23341 tggttgaatc gtattgaaca agtaactgat taccgctttt tgcgcaaaga acgtgttttt
    23401 acaggaaaag ttattaatca agtattgcta acgaaaaaag atatagaacg attgactcga
    23461 ttgtatcatt atcgagtaga tttagaagag gatttaactt tgtcaattta tcgcgttttt
    23521 tctcctgaaa aatatagtga aataacaaaa ttagatcatt taattttgta gttattatct
    23581 atcgggaggg ctctagacgt agtctggtag ccgcactctg cctggcggtg cgcattaagg
    23641 ctgcgaagca tgggcctata atgcgcactc gctagcagcg tgtgcagctc acaaagaggt
    23701 cgtgcgcttg cgcacggcct ctttgtgtaa agccccccga tatataatat ggggggcaga
    23761 aataacataa cacatagaaa aaagttttat aaaccctttt gtatcaagtg attatctaag
    23821 gtcagcagtt gtactacttt tcccaaaaaa cgagggggac tagttgatat atcggtgttt
    23881 ctaaagggtt aaaaaaaatc gattttgtta ttttataaaa ggagcgagaa aatgacaccg
    23941 attgagctaa aaagctacag aaagaagtta agtgcaattc ataatataag tggagataaa
    24001 aagccgctat cacaagcaag atttgcgcaa ttgatgggct gctcaaaaga ttacattaga
    24061 aagatggaac aagggaaaag aaaaatccca gaggattttg aacaacgaat caaggaacgc
    24121 tttcgattgt atatgaaaag cggaatagat cttcgagcaa tgattgatta tttgcgtctt
    24181 tccttttttg ggacagacta caaaacagtg attcaacgtg tattacgaat ggatctgaca
    24241 gaatttgaaa cacgtccaac gggcttgtat ctttttgact gtctttctgt tcgtgggaat
    24301 atctgggtgt tctggcattc aaaacgaact gtaacagaaa atgtacttgt gcaattttca
    24361 ggacaaggtt gccgggaata tgaggaagtc atgcgtgaac aacaaacaga ttggcgaaca
    24421 tggctatatt ccatttggaa aaatcaagga aagttggaag ctggttatac acgtgtgcaa
    24481 tgtagccgat tcgatattgc gttagatgaa ctttggggat tggaaacgga agaacatttc
    24541 gatctctttg aattactaga aaaacaaaaa gcaggtttat tagaaatgga ctttaagaga
    24601 ttccagaata ttggcgg
//
LOCUS       DACIRY010000033        24426 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.033, whole genome shotgun sequence.
ACCESSION   DACIRY010000033 DACIRY010000000
VERSION     DACIRY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.033"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(188..2533)
                     /locus_tag="IYE22_001150"
     CDS             complement(188..2533)
                     /locus_tag="IYE22_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ9506372.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            2812..3714
                     /locus_tag="IYE22_001151"
     CDS             2812..3714
                     /locus_tag="IYE22_001151"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ9506373.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            3927..5702
                     /locus_tag="IYE22_001152"
     CDS             3927..5702
                     /locus_tag="IYE22_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ9506374.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5768..7354)
                     /locus_tag="IYE22_001153"
     CDS             complement(5768..7354)
                     /locus_tag="IYE22_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ9506375.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7605..7829
                     /locus_tag="IYE22_001154"
     CDS             7605..7829
                     /locus_tag="IYE22_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ9506376.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7971..9722
                     /locus_tag="IYE22_001155"
     CDS             7971..9722
                     /locus_tag="IYE22_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9506377.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            9722..11506
                     /locus_tag="IYE22_001156"
     CDS             9722..11506
                     /locus_tag="IYE22_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506378.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            11832..12683
                     /locus_tag="IYE22_001157"
                     /pseudo
     CDS             11832..12683
                     /locus_tag="IYE22_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12737..14488
                     /locus_tag="IYE22_001158"
     CDS             12737..14488
                     /locus_tag="IYE22_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9506379.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            14492..14662
                     /locus_tag="IYE22_001159"
     CDS             14492..14662
                     /locus_tag="IYE22_001159"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506380.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(14702..15604)
                     /locus_tag="IYE22_001160"
     CDS             complement(14702..15604)
                     /locus_tag="IYE22_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9506381.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            15835..17238
                     /locus_tag="IYE22_001161"
     CDS             15835..17238
                     /locus_tag="IYE22_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ9506382.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            17238..18671
                     /gene="ascB"
                     /locus_tag="IYE22_001162"
     CDS             17238..18671
                     /gene="ascB"
                     /locus_tag="IYE22_001162"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ9506383.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18816..19742
                     /gene="trxB"
                     /locus_tag="IYE22_001163"
     CDS             18816..19742
                     /gene="trxB"
                     /locus_tag="IYE22_001163"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ9506384.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19956..21683
                     /locus_tag="IYE22_001164"
     CDS             19956..21683
                     /locus_tag="IYE22_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ9506385.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            21755..22048
                     /locus_tag="IYE22_001165"
     CDS             21755..22048
                     /locus_tag="IYE22_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ9506386.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(22140..23000)
                     /locus_tag="IYE22_001166"
     CDS             complement(22140..23000)
                     /locus_tag="IYE22_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ9506387.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            23092..23520
                     /locus_tag="IYE22_001167"
     CDS             23092..23520
                     /locus_tag="IYE22_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ9506388.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23682..23858
                     /locus_tag="IYE22_001168"
     CDS             23682..23858
                     /locus_tag="IYE22_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ9506389.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23885..24331
                     /locus_tag="IYE22_001169"
     CDS             23885..24331
                     /locus_tag="IYE22_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ9506390.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7791 a         4315 c         5167 g         7153 t
ORIGIN      
        1 ttatgacaaa attttgttat aatccttttt ttgtgtctta aaaaagagag aaagtggttg
       61 aaattacatt tatcgtatat aaaacaatcc gtgtatagat gctgcatttt gttcaccacc
      121 atcaaaaaaa taagggactg tgacaatact attgtcacaa tcccttatta ctgtgtattc
      181 tattaaatca atcatctttc tcatctgatt ctttagttgc ttgattcgct cttgcataag
      241 ctgaagcggt gttccagtaa tctgtatagt tttcatcggt accgccaacg ccaaatttaa
      301 aggcgctttt cttcggtcca tttttcgccc atagcgattc aactgttttt cctggtgttt
      361 ggatggattt tttattgaca gtaacttttc caggtgtttg accagtaaat ccagatactt
      421 ttttcttcat cacatcagat gataattcga atttctcatc agtgccaaaa atgtcaggtt
      481 ttgcttggta agcttgattg atcagattgg caagataatt agccgaacgt atccttgctt
      541 tgctgtccat tccagtattg tcatcgtgcc ctgcccagct actgatggta atagacggag
      601 tagagacaat tagccatgaa tctcggtatt cattggtaga accagttttt ccgacccaat
      661 ctgcttttcc tagatttccg tttaagcttg agatggcatc tttgaatggt gtagtgattt
      721 ttgcattgat cacactacgc atcatgtcat tcatgatcga tgccgttgct tccgaataga
      781 tccggacaga gttgctttcg tgtttataaa ttacatttcc tgcgttatct gtgatggaat
      841 cgatgatata gccttcttca tagacgccgc cgtttgctaa agcctgaaat ccgttggtct
      901 gttgaagcgt cgtgacatct actgtaccta aaggagcaga ttcaacgccc caattgtcat
      961 ttgccgggta gttcatcttt gccaagtagt gctcgtaagc atattgtttc gatccaccgg
     1021 aattcaacac atcttgatat aagtgataag ccgggatatt gttggaccat tctagtgact
     1081 cacggacggt ttggaaggtg ttcgatcctt cgttagtagc attgacgatt ttctcgccag
     1141 cattttctcc ttcttgccaa gttgttgcat aatctgatac acgggattct gatccgataa
     1201 gccccatgtc gattgcaggt ccataaacaa gaactggttt gattgatgag ccagcttgac
     1261 gttctgtatc gaaggcatgg ttattttgat tctgactgta attccgtccg ccaacgaatc
     1321 cgtagatgcg gccagttctg ttatccatca atacatttcc ggtttctact tgactgccag
     1381 caccatcatc taacatgtat ccataatttt caacgccatt ttgcaatgca gcatagacat
     1441 ctttatcgat tgtcgaatgg attgtatatc ctttattttg gatcgtttgc tgtgcctttt
     1501 gataataagc gtcactgacc gatgaatcag acatatcagc gttgtctttt tcagccaatt
     1561 gttgtgcaat gattcttgtt gcttcattca tcacagtata atacaagaat tctctgtcag
     1621 tctgttctgc gatttgttgt ggtagaaagt cttttgtaag gtcataggct tttgcttctt
     1681 cgtattgttc tttcgtaatt tggccttcac gatacatact gaataaaaca aaatcttttc
     1741 tggcaaggcc ggcagaaaga tcttctttta gcgcgcctgt gttcgtgtat ggactatacg
     1801 tgatcgggct ttggggtaat ccagcgatat aagcagcttg cggcagcgtc acatcttttg
     1861 catctacacc aaagattcct tttgcagctt cctggactcc agcaatgttt tgtcccttat
     1921 tattacgacc aaatggtgag acattcaaat acgtagcaat gatctcatct tttgaaaagt
     1981 atttttctac ttgtgcagaa agaatgatct cgtttgcttt acgtttgaat gttgtttcat
     2041 ctgtaaggat ctgctgtttg actaattgct gtgtcaaagt tgatccacca gaagagccaa
     2101 taccagtagc ttcagatact aatgctcgta aaacagcttt aggaacgtaa ccttggtgtt
     2161 tatcgaagta ttcatcctct gtgctgataa ttgcagtttt caataaaggt gaaatcttat
     2221 cagatgaaat agtcgtgcgc atcagatctg tctggatttt tgatatctct gtattatctg
     2281 cataaacaat ctttgacgtt tctgtaatgt ttcctagttc tgtctgcagt gcttttttag
     2341 tcggcaattg tgtgtcttcg acgagatagg caaaatagcc agcgcctatt ccaataccta
     2401 gagctgcgaa catcaaaaga agaacagtaa taaaaacagt caatgattga aaaactctaa
     2461 aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt tttcgagtat
     2521 ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt atagcaaata
     2581 atagaacaaa aaaacagaca aggctgtttt tggcaggaat ttctttttta aaagagttaa
     2641 aacgtgagaa aatgatcttt attataaaag agttcttaag cttttcttta gagaaaattt
     2701 cagttactat ggagaacagg gcaaagaaaa atcaatcatt ttctttgtat atcatcttac
     2761 caatatatag gaattgttag taacaattgc ctttcaagga ggaatccatc catgttatta
     2821 ggctcacacg tcagcatgag cggaaagaaa atgctgttag gagcagcaga agaagcagcc
     2881 agttatggat cttcgacttt tatgatctat acaggcgccc ctcaaaacac tagaagaaaa
     2941 gccatcgaag agatgaatat tgatgccgga aaagcgtaca tggcagaaaa aggtctaaca
     3001 aatatcgtcg ttcatgcccc ttatatcatt aatttgggga atacgatcaa accagaaatg
     3061 tttccttttg ccgtccaatt tctgagagaa gagattgttc gtgcggaagc tttaggcgct
     3121 actcaaatta cacttcaccc aggtgctcat gtaggggcag gaacagaagc cggaattgct
     3181 agaattattg aaggcttgaa tgaagttttg acaaaagaac agacggcaca aatcgctttg
     3241 gagacaatgg caggtaaagg tacggaaatc ggtcgaacat ttgaggaatt agcgcaaatc
     3301 atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgacacctg tcatacgaat
     3361 gatgccggtt ataatattaa agaagacttt tcaggtgtaa tggaagagtt tgataagatc
     3421 atcgggttag atcggcttaa agtactccat atcaatgatt ctaaaaatcc gcaaggttcg
     3481 cataaagacc ggcacgccaa tattggtttt ggaacaatcg gattcgacac gctgaataag
     3541 atcgtccatg atccaatgtt tgacaatatt tcgaaaatct tggaaactcc ctatgtaggc
     3601 aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tcgccatgtt caaagcgcaa
     3661 acattcgatc cagaagtgtt tcctgagtta ttagcagaag aagtgatact ctaagatttt
     3721 ctatctgaca gcagcaaaaa aggaccaggc acattgcctt gtcctttttt gtttaagaaa
     3781 caaaggagta ctttttgcag tctttttcat catgcatctt ctcccccctc tttttaaatt
     3841 tagacaaaca aggtatactg gtagaaaagt aaaaaaacga aaagtttcac aagcaagtgg
     3901 caaaacgtga ttggaaggac gaaaagatgc tgaacacttt aaaacccttg atcaaccaac
     3961 taaaaaagaa acatacatac tttattcttg gcatagccct attatttaca attattcgtt
     4021 tgtatttata tagtaaagca atctatggaa cagacatgtc ggcaacctat gatgaccagc
     4081 tgctgattcg ctatgctcaa aatattttgg acggtaaatg gctaggggag tatacgacta
     4141 caacactttc caaaggaatc tcctacagct tatttttagt acttgcacat aaactgcatc
     4201 tttcttatgc agttttgttc ggcatgttca gtatttttgc cagtatattg gttgcactcg
     4261 cgtttaaacc gattgtgaag aacaagtatc ttcttttaag tatctatttt tattttttgt
     4321 tttctccgat tagttttaca catgaatatt ccactcgaac atatcgtaat gccctcgtga
     4381 ttccggctgt tgagatcatt atcgcttgtt tattagctat ttattaccgc aaatacggtt
     4441 ctagcagaaa attgctgcca tggtttttag ggctttctgt cgcttttcct ttcttttggt
     4501 tcattcgcga agattctatc tggcttcttc cttttactgt agtagctttg ggtatcagtt
     4561 tcgtccagat cttgctaggg caagactttt ctttcaaaga agtaaagaag ttaaaaaaac
     4621 gactctttct tctctcgaaa cgggaatggc tgaaactttc attgttttta cttcctatag
     4681 ttggcttttc catcgtctat caatggatcg aatataaaaa tgagacaaca tacggtattg
     4741 caaccatcaa tgaccgttcc agtggagaat ttggacgttt gatgaaacaa ctgatccgaa
     4801 tagatgatga aacgaacttg aataaagaaa acagtgagat atgggtctca catgctgcac
     4861 taaataaagc attagccgca tctcctacat tccatactgt ttctacccgt attgacgaat
     4921 tatatacgac tcacgcgtgg actcaaggtg gaaaagtaaa agaattaccc ggagatatca
     4981 tcttttgggc attgcgagat gtggtaagcg aatctggtta ttaccaaaac aatgcaaaag
     5041 aaaccaatga tttttggaaa aaagtcaacc aagaattaga agaagcctat cacaagggaa
     5101 caatcaaaaa gaaaaaagag ttctatctga tgggatcagg agatggaaag acgatgagtg
     5161 atctgccaat cgtattcgat ttccttcaat caggatacgc ttatgcatgg cgatataaag
     5221 atttccatca aggaggaaat tatagtttcg gaacgcaaga acaagtcaaa caggctcaag
     5281 aattattaaa ccttccgcta atggagaatt ggatcgatag taatgaaccg agaacaccta
     5341 atttccaatt aacaaaatca gcaaaagtcg cgaatgtcat cataaaaatt taccaagttc
     5401 tggctattcc cgtgctgatt gcttcaatag cagggatatt acttatgata ctaggaaccg
     5461 tatttgcaaa agctgatcgt gcctattata gttcctcttt gattgtggtc accggattaa
     5521 tactgacaca attggttttt cttttcggtg tctcatggtt ttgttcttat gcgccagaaa
     5581 aaaaggacta ttttttaagt gtctacactg gtgcaggcgt gccattaacg caattcatcg
     5641 tagtaacaag catgctagtt ttcagtaaat taaatctgaa aaaagcttta tcttctaaat
     5701 aaaaagaccc ttggtagcta gatttttaac ctaactatca agggtctttg tgtggaaaaa
     5761 tcagttttta ttcgatttct tctaccgttt cctgcaaaag ctgttcatat tttttgtttc
     5821 ctttatataa atcaccaatc gtccatgcca acaggagaag gacaagaaca atgattacat
     5881 aagcgatgtg atcagccaat gctagttgag cggaagtggc ttgctcgccg aagaaacttt
     5941 cgatctgtcc tggtaatcct cgtaagttca agtaagttag actaatcaca gagaaccagc
     6001 caagaagttt tacgatgaat gaatttttaa atcggctacc catctccgct tggctgttgg
     6061 tcatcatcag taaaggaatc atcgaaaacg gcaacgcaaa agccaagaac acctgggatt
     6121 cattcattaa gttattcaaa gcggcatgtt cttcgatcgt tccttttgta cttgtaaaca
     6181 aaacacaaat caatacagga atcacagaaa gcaatcgtgt gaccagtctt cttagccaaa
     6241 tcgggatacg catatggatg aatccttcca tgatgacttg accggtcaac gtaccagtaa
     6301 tagtagagtt ctggccagaa gcaagaagtg ctacagcaaa taaagtggac aagataccag
     6361 attttgctac agcaatcaaa ataccattgc tcatagctga tggatcagat aaagcttcat
     6421 acagaccaaa aaaggaagga tcttggacag aaccggtttt gaagacagcg acacccatga
     6481 tcaatagcag tgcattgact agaaaagcca ttgacaactg gatattagaa tcccaagtag
     6541 aaaaacgtac agcttgtgct acatcttctt cgttttggtg atcgatcttc cgagtttgtg
     6601 aaactgctga atgaagatat aagttatgcg gcatgactgt tgcaccaata atccctaaag
     6661 ctccagtcag cggagtttcg ccgccgatga ccggtgagtc tgagaatgta tgactgttag
     6721 ggataaagcc agcaaataaa ctgtgccaat ctgggttaga caagccaact tgatagacaa
     6781 aaacgaataa aatgacgaaa attaagcaga caaccagtgc ttcgatctta cgaaacccga
     6841 cttttgtcag tagtaagaga acgaaaacat ctaatacagt aataaagacg gcaatcacca
     6901 atggaatatc aaaaagtaag tagagggcga ttgctgcacc aatgacttct gcaatatctg
     6961 ttgccataat ggctaattca gtcaagatcc ataaaatgac acctaatgtc ttacttgtgc
     7021 gtgctcggat tgcctgtgcc aaatccattt ggctcacgat tcctaatttt gctgccatat
     7081 actgtaaaag catagcaatc agactagaca tcaaaataac tgacatcaat aaatattgga
     7141 aattttgtcc tccggtgatc gaagtagacc agttacccgg gtccatatac ccaactgcta
     7201 ctaacgcacc aggtccagag taagcaagaa gtgttttaaa aaaacttttc ccctttggta
     7261 catcaactgt tccatttact tcagctaaag ataaaccatt tgtatgttca ataagtcgat
     7321 gcttatgaaa ttgtttattg gattgattat tcaagaaaaa accacctttc ctatttatct
     7381 acgattacat tctacacctc aaaaagaaaa aattaaagaa aaagattcgg tacgcctaat
     7441 tttttttcat ggcgcgtctt gctagatgac gaaagaaatt ttgttctcct ctaaaaaaac
     7501 cactattttt tgcatgaaac taaagaatac gcctttcttt tttcaataaa ctttagtaaa
     7561 ataagtcatt gagagcaatt cggaaataga aaggagagat ttatatgtta tcaacaggta
     7621 ttgtcgtact tattgctatt attgctttgc taatcggcgc agtaggtggc tttttcctag
     7681 cgcgtaaata tatgaaagac tatttagaaa aaaatcctcc cgttaatgag gacatgttgc
     7741 gatcaatgat gatgtcaatg gggcaaaaac catctgagaa gaaaattcgc cagatgatgc
     7801 agcaaatgaa gaaccaaggg aaaaaatagg ttctgaccca acgacttttt acgggctgat
     7861 tttttcagtc cgttttttat ttcgtaattt gttagactat tcagattatt cagcaaaaaa
     7921 gacaagcaca gacaatccta ggctcttgat aaagacaaag gaggagtaaa atgtcgattt
     7981 ttagaaaatt aggctggttt tttcgtcagg aaaagaaaca ttatttaatt ggggttttct
     8041 cgctattcgc agtggccttg gtacagttag ttcctccaaa agtgattggg atcatcattg
     8101 atgagatcgc cgataaaaat ttatcaataa agatcatttt cgggtggatc ttcctattga
     8161 ttatcgctgc ttttttacaa tatctttttc gttatatctg gcggacgaat atttggggaa
     8221 gtgcagctcg attggagaaa gaacttcggc agcagttatt caaacatttt acagaaatgg
     8281 acaatctctt ttaccaaaaa tatcggactg gggatttgat ggctcacgca acgaatgatt
     8341 tgaatgcgat ccagaatgta gcaggagctg gtatcttgac ctttgcagat tcagtgatta
     8401 cgggcggaac aacgatcatt gcgatgattt tgtttattga ttggcgctta acactgattg
     8461 cgctgattcc tctgcctttg ctggccgtca cttcacgagt gctggggtca aaactccatg
     8521 atgcttttcg tgattcgcaa gaagcttttt ctagtatcaa tgataagaca caagaaagta
     8581 tcactgggat caaagtcatt aaaacatttg gacaagaaaa agaagatcta gcggattttg
     8641 aagaaaagat tgaagatgca attattaaaa acaaacgagt caatttttta gactccttat
     8701 ttgatccatt cattaccttg attatcggtc tgtcttatgt tgcaacaatt attttaggtg
     8761 gaaagtatgt cttggaaggc acgatctcga tcgggcagat ggtctcattt attagttata
     8821 tcggtatgct tgtttggccg atgtttgcga ttggtcgatt gttcaacgta ttggaaagag
     8881 gaaatgcttc ttatgatcgc gtcaatgaac ttttgcatga aaagacgcat atcgtggaac
     8941 aactgggtgc agtgcagacc ccggcaaaag gcgagctgaa tgtagctatt tcctctttta
     9001 attatccgaa agaagaatca ccagcattag aacaaatcaa gtttgcgatc caagaaggag
     9061 aaacattagg tattgttggg aaaactggtg cgggaaaaac gacgatcatc aaattactga
     9121 tgcgtgaata cgatgattat caagggaaaa tccaatttgg cggacgttcg atcaaagaat
     9181 attcattgaa tgctttatta ggatcaatcg gctatgtgcc tcaggatcac ttcttgtttt
     9241 cgatgaccat tcgagataat atccgttttt ctaatccaaa actttcagaa gaagaagtag
     9301 agcatgcggc ttctttagca tttatcaaca atgaaatcaa ggaattacca aaaggttatg
     9361 ataccttagt tggagaacgt ggagtctctt tatctggcgg acagaaacaa aggatttcga
     9421 ttgctcgagc attgatcaaa aatcctgaat tattaatatt agatgatgca ctctctgcag
     9481 tagatgccaa aacagaagaa gctatcttgc ataatttaaa agaaataaga aaagagaaaa
     9541 caactatcat tgcagctcac cgtttaagta gcgtgatgca tgcaaaagaa atcattgttg
     9601 tcgatcaagg gaaaatcatt gaacgaggaa cccatcatga gctgcttgca ctaggcggct
     9661 ggtatgaaca aatgtgggag aaacagcaat tagaagcgaa gatagaagga ggcgaggcct
     9721 gatggatgaa aattatcaat cggaatggac aaaatcgatt ccacttaaag aacagaaaaa
     9781 gatcatcaaa cgactgatga agttcgccaa accttttaga agaacgttca tcgttgctat
     9841 cttatttgcg tttgctttgt ctgtgatcaa tgtcctattg ccaaggatca tccaaacttt
     9901 tatggatgat catctggcta aacagtcggc tacgacacaa gttattttgt tttttgctgg
     9961 tatgtactta tttggagtca tagtaaaaag tatcatttgg tttttccaat ggtatttgta
    10021 ttccatggcc tcattgaaga cctatcagta tattagggta aaattatttg aaaagctgca
    10081 tacactaggt atgcgttatt ttgatcagac accggcagga tcaaccgttt ctcgagtgac
    10141 gaatgacaca gaaacgctat ttgaattttg gtacgtcttt ttgatggtat tgactgggat
    10201 ctttgcagtc gtttcttcat tccttgccat gtttcaaatc aatggagaaa ttgccttgtg
    10261 gactttggtg tttttgccga ttttaggaat cgtcatctgg tattaccaga aattcagttc
    10321 tcgtatttac cgaaatatgc gggaaaaatt aagtcagctg aacacgaaat tgaatgaatc
    10381 gatttccggc atgcgtatta tccagcagtt tcgccaagaa gctcgtttag ctaaagaatt
    10441 tgaacaagtg aacaatgagt atttagacac gcgttatgcg atgatcaaaa cgaattcgat
    10501 tctgttaagt ccgatcatca attttctata tgcattggcg attgcgttgg tgttgacttt
    10561 gtttgggatc gatgcgttgc attctcccgt agaagttgga ctgatctatg catttacgac
    10621 ctatgtccag ggatttttta atccaatgtc tcaaatgatg gactttttga gcatcttcac
    10681 agatgggatg gttgctggta gtcgtatctt gaagattatg gatacggaag aatatgcacc
    10741 acaacaaaac cctggagcag atcaaatgat tacttcaggt aagattgagt ttcgcaatgt
    10801 aagcttttct tatgatggag agcacaatgt attaaacaat atctcttttg ttgctaatcc
    10861 gggagagacc gttgcattag tgggacacac cggaagcgga aaaagctcga tcatcaacgt
    10921 attgatgcgg ttttacgaat tttatgaagg tcagatattg attgatgatc atgacattcg
    10981 agaatatcca atggaagagt tacgtaagaa aatggggctg gtcttgcaag atgctttctt
    11041 gttttatggg gatatcgcag gaaatattcg gatgatgaat ccagctatca cagacgaaca
    11101 gatacgccat gcggctgagt ttgttcaagc aaacaaattc atcgatgaat taccaaaagg
    11161 atatcactca aaagtgatcg aacatggtgc cagctattct agtggacaac gacagctgat
    11221 cacttttgcg cgaacaatcg tgactgatcc taaaattcta gtattggatg aagcaacagc
    11281 aaatatcgat actgaaacag aaggattgat ccaagaagga ttggcaaaaa tgcgtcaagg
    11341 acggacaacg attgcgattg cccatcggct gtcaacgatt cgagatgcag aattgatttt
    11401 agttttagac aaaggtcata tagtagaacg cgggaatcat gaaacacttc tagcgcaaaa
    11461 tggtttgtat gcagatatgt acaaactgca aagcagtggt atataaaaga tgttgtgaca
    11521 gtagaatttt gacctaagca ctagagtgaa aataaaacat agcttctcat atttttcatc
    11581 tgtgagagtt agtgtcgtag tcaccagatt taaaaaaaga ttcgacagct agtcagaagt
    11641 gcctaatagc cactaagaaa gcgttgacaa aagcatttgc tttcgataat ataaagaaaa
    11701 cgatacaact tggatagtga ttgcctgatg caagctaaac ctgatgagtc tcccatggtt
    11761 tcatggacgg ctttttgggt ttttcttttt gttttggata cagccctata catcatggaa
    11821 aggagcaaaa catgctgatt gagaaagtac tgaataacaa cgtcgtaata accagggatg
    11881 aaagaaatca ggaaatgatc gtgatgggaa aaggtttggc attcaaaaag aaagtgggtg
    11941 acccgatttt tgactcatgc atcgacaaaa aatttcagtt gacaaatgac gggctttcac
    12001 aacagttcta agaattggca caagcgattc ctcttgagta cttagaaatt tcttgtgaaa
    12061 tcattgaata tacaaaagag gctctacaag tccagttgaa tgattctgtg tatattgcat
    12121 tgacggatca tttgtatacg gctatcgaac gtcacaaaat gggaatattt gtaccaaatg
    12181 tattgctgta tgatatcaaa caactatttc ctaaagagta tgccatcggg aaaaaaacag
    12241 tgaagaaaat ctcggataaa taccacatca agttatcaga agatgaagcc ggatttatcg
    12301 cattgcattt agtcaatgca caaggcattc aaagccagca agacaatacc tacctgttta
    12361 ctataacaat acaggatatt ctaaatatcg tccgctatta ctataagatt acatttgatg
    12421 aagattcagt ccattttttt cgattcgtca cacacttacg tttttttctg acacgcgtcc
    12481 tagatggaga aaaatcagaa gtgggagaag tttctgagga attgtttcag gttattcaaa
    12541 aaaaatataa ggatacgttt ggttgcgttg agaaaatagc agacttttta gccgagacgt
    12601 tcaactacca gatgtccacg gacgaaaaaa tgtatttaac gatccatact gcgcgtttag
    12661 tgcatgagaa tcgttgtaaa taaaaaaagg aggataaatt aacaagaacg tatgaaataa
    12721 gggtagtgac attcagttgg ctaaaccttc cactaataaa ttggagggaa tagaaatggg
    12781 aaaataccaa gaattagcga gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa
    12841 tggtttgaca cattgtatca caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa
    12901 tgatgagatc ttgaaaaata tggacggtat tgtaacggta atgcgagcgg gagggcagta
    12961 tcaagttgtc attgggaatc atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa
    13021 ccttactttt gatgaggctg tttcaacaga aaaaatgaga ccgtttgata tgcttattga
    13081 tggaatatcg ggctgtttcc agccattcct tgctatccta gctgctggcg ggatgattcg
    13141 tggactgaca gcattccttg tcttcttagg cgcgtttgac cgcggttctg gcacatttgt
    13201 tatgtttgat aatatcggtg attctgtatt ccaatttatg cctgttatta tcggtttaac
    13261 agctgcccgt aaatttaaag taaatgaatt cgtagggatg ttgcttggcg cagcattaat
    13321 gaatcctagc ttgtcgctgg aagctttgag tggtgcagca gaagcaccac taacgaccat
    13381 attttctggc actatttttg aagcacctat ttatcaaaca gtattcggca ttccctggat
    13441 tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt attgtgttgt tagctagcca
    13501 aattcagaaa ccaattaaaa agttagtacc tgagatgatc gcgaatttct tcgttccatt
    13561 cttcaccgtt cttattacaa tgcctttagg ttttttactc gttggaccag tattcacatt
    13621 cgcaactgat attttaatgg ctggatttga aacactttta gcactttcgc cagttattta
    13681 tggagcaatc gtaggcttct tctggcaaat tctggtgatg tttggtctcc attgggcaat
    13741 cgtaccaatg ggattgatgc aattttctgt gaatggttgg caaaatatta tgacacctgt
    13801 tgcagtagtg tcttttggac aaactgcagc acttaccgca ctctacttta agcttagaaa
    13861 tccaaaagac aaagcaattg caataccagc cattgtttct ggtattgtgg ggattacaga
    13921 gccagctatt tatgggttta cattaccacg taaaaaaata tttattttta cctgtgtcgg
    13981 tggtgcgtta gggggcgcgt atagtgggtt aatgaattta acttcttgga atcaaggtgg
    14041 actaggcatc tttacaattc ccaactatat tagaccagat ggtgatttaa cagatgtgat
    14101 taatgtattg attgggatcg ccattgcgat ggtggtatcc tttacgctga cttttttctt
    14161 ttggaaggat gaagcaggtg aaacagatga tatacagaaa aaatcaggta aagaaattgt
    14221 aaaaacaccg atacaaggac aaatcgctcc actaaatgca gcaaaggatg cagcttttgc
    14281 acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc
    14341 ttttgacgga acagtcatga ccctgttccc gacgaaacag gcaattggaa tggtttctga
    14401 aacagggctg gaattgctga ttcatgtcgg attggatact gtgcagttgg aagggaaata
    14461 ttttgaaagc ttagtgcagc aaggataaag agtgaaacaa ggcgatctgt tgctacgctt
    14521 cgacaaaaag aaaatcgaaa aggaaggata cagtttagaa acacctgtga tcgtcaccaa
    14581 ttacacggat tatttggata ttttggaaaa ccaaggggag acggtcgggc cagacgatgc
    14641 acttatcacc gcactgacct gaacggttag gtgaagaatg gactaggctt aactattctt
    14701 tttattccac ttctttattt tcaaaattga aaggctcttt catgatggct gagctgatag
    14761 ggcgtttatc gaatttttga ccgacagaaa ttagatggac taaattctgt tgataatttt
    14821 ctgcaaaaac acaactatat aacacgtcta gaagataaca aattgatgta ttgatcgtaa
    14881 aattaccaat ttttgaatac agtcgctcgc gtgtcgtaag tcgcaaagcg gctgtactca
    14941 ttgcagatag cgttgaatca ccgatactgg tcaaagctaa aattggaaca tgattttgtt
    15001 tcaatatttc tgcggtacga aggatcacat tgttttcacc cgtataagaa atcagaatcc
    15061 cgcacatatc actcgtgcaa ttgtacgctt catagaatga ttcaccaaaa gtggtgacaa
    15121 tcgtgacatc cattttgatg cgcttcattt tcagcatgaa atcttgagaa atcaaagtat
    15181 tcgagttact ggcgaaaatc acgatttttt ttgcttgaag cattagttgt ttggcttttt
    15241 gcagttggtc atgatgaatg agagaaagtg tatcatgaat cgtcatctgt tctaaagaag
    15301 ccattttatt tgcgatcgtc ataataccgt ctgtggtgga aaaaggaaga ttcgcatcta
    15361 cgtcttgaaa attcccttca agataggctg tttcagctaa aaaagcatta cgaaaactga
    15421 cccagccatc aaaacccatt ttttttgcta cacggatgag tgtagagggg tggacataac
    15481 atcgctcagc aatcgcttgg atggtcattt cgccaagtgc tggagaatcg cttaagagaa
    15541 actggatcac ttcattttct gaaggagaaa aggagatttc tttgattttt tcacgtaata
    15601 acatgtagat accttcttct tattttttta cgtaaattat aacttttttg aaaatgattt
    15661 gtaaacggat ttatactata taacttaaaa tagacatttt ttgacaagat atttacaaaa
    15721 atccactaga aaacagtaaa ttttgtaaat gttactggaa agaaacagat ttctattgtt
    15781 tgttttgttt acaaaaaaga taatgaaagt gtaaaaaagt aaaggagtga gaacatggca
    15841 aaaggaatca aaatcgctac gattggtgga ggcagcagtt acacccccga gttgatggaa
    15901 ggatttatca aaagatatga ggaattaccg atacgagaaa tctggctcgt agatgtagaa
    15961 gaaggaaaag aaaagcttga gatcgttagt cagatgtctc agcgtatgtg ggatgcttct
    16021 ccgtatgaag tcaagatcta tgcaactctg gatcgggaag aagcattgaa agatgcggat
    16081 tttgttacga ctcagtttcg tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc
    16141 cctagttatt atgggatgat tgggcaagaa acaaatggtg ctggcgggat attcaaagct
    16201 tttcgtaccg ttccagtcgt tttggcaatc gttgaggaca tgaagcgttt atgtccaaat
    16261 gcttggctga tcaatttcgc gaacccaagt gggatggtga cagaagcagt tatcagatat
    16321 ggcaaatggg ataaagtgat tggattatgc aatgtgccag tgatggcgat gatgacagaa
    16381 ccagcaatgc ttggcaagaa aaaagaagag ctgatctaca agtttgccgg tctaaatcat
    16441 ttccattggc acaaagtagc agataataaa ggaaatgacc gcacggcaga attgattgat
    16501 cgtttatacg atgaagagaa tggattaccg aaaaatatct ttgatatccc gtttttcaaa
    16561 gaacaattaa aacaaatgaa catgatccct tgtggctacc accgctatta ctatcgtgaa
    16621 gaagaaatgc tggaacatgc tttagaagaa tatcaaacga ttggtacaag agcgcagcaa
    16681 gtgaaagaaa cagaagcaga gctatttgaa ttatacaaag atccagaatt agaccataaa
    16741 cctgaacaat tgcaaaaacg aggtggcgct tattattcag acgctgcctg tgaaacgatt
    16801 gcttcaatct atggaaataa agaaacacag atcgtggtat ctacaaagaa tgatggagct
    16861 gttcctgacc tgccggcaga ttgtgtagta gaagtcactg cctatgttgg cgcacaagga
    16921 gcgagaaatg tagcgtttgg ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg
    16981 aagaatatgg aattgctgac gatcgaagca gctgtgactg gtgattatgg gacagcttta
    17041 caggcgttta cgatcaatcc acttatacga tcaggaaaga cagcaaaacg gatcatggat
    17101 gagttgttta ttgcacacca gcagtattta ccgcaattca aggagacaat cagccgatta
    17161 aaagaagaag gaatcgaagt tcaagatgaa ctggcaagag aattatcagg taatacacat
    17221 ataatggagg aagtataatg ggattcagaa aagatttttt atggggcggt gctacagcag
    17281 ccaatcaatg tgagggcggt tatgatgaag gtggacgcgg actagcaaac gtagatgttg
    17341 tgccaatcgg aaaagaccgt tttcctgtta tcaccggaaa gaaaaaaatg tttgaatttg
    17401 atgaggatca tttttatcca gcaaaaattg caatcgatat gtatcatcgt tataaagaag
    17461 atattgcttt gtttgcagaa atggggttca agacttatcg aatgtccatc gcctggagtc
    17521 gtatttttcc aatgggggat gagaaagagc cgaatgaagc tggcttgaaa ttttatgagg
    17581 atgcctttaa agaatgccaa aagcacggaa ttgagccact tgtgacgatt acgcattttg
    17641 attgtccaat gcatttgatc aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt
    17701 tttatgaaaa tctgtgtcga gtcatcttca atcgctataa aggacttgtc aaatattggc
    17761 tgacattcaa tgagatcaat atgatccttc atgcaccatt catgggcgca gggctgtatt
    17821 ttgaaaaagg agaaaatgaa gaacaaatca aatatcaagc ggcacatcat gagttggttg
    17881 caagtgccat cgcaacgaag attgctcatg aagtagatcc ggagaacaaa gtaggctgta
    17941 tgctggcagc aggagcatat tatccataca cttgcaatcc tgaagatgtt tgggcttcta
    18001 gaaaatcaga tcgagaaaat tatttcttca ttgatgtcca gtcacgagga gaatatcccg
    18061 cttatgcatt gaaagaaatg gagcgaaatg gcattcagat cgaaatggaa ccaggggatg
    18121 ccgcattgct gaaagaacat actgttgatt ttatctcttt ttcttattat gcttcccgag
    18181 tttcgactac cgacaacgaa ctcttggaac agactgcagg aaatatcttt gcttctgtca
    18241 aaaatccata tctgcaagca agtgaatggg ggtggcaaat tgatccatta ggcttgagaa
    18301 taacgatgaa tgatatttat gatcgttatc aaaaaccatt gtttattgta gaaaacggct
    18361 taggtgcagt cgatacgcca gatgaaaatg gttatgtgga agatgattac cgaatcgatt
    18421 acctggcagc gcatatccag gcgatgaagg atgcagtaga actagacgga gtcgatctat
    18481 taggctatac gacttgggga tgtattgatt tagtatcagc tggtaccggt gagatgaaga
    18541 aaaggtacgg attcatttat gtcgatcgtg ataaccaagg aaatggcaca ttagcccgtt
    18601 caaagaaaaa atcattcaac tggtataaaa aagtaattga ttcaaatgga gaaaatcttt
    18661 cgaacgattg atcaatgatt tataaaagtc tattgttttt cagcggattt ttcgagtata
    18721 ataaagctat tcgagaaacg caagcagaac aatagacttt ttttcgcatt ggctgtaaag
    18781 tatgcttact tgattaagta aaggagaatc tacatatgta tgatgtaatc gttattggcg
    18841 caggtcctgc tgggatgact gctgctttat atgcttcacg ttcaaactta tccgtattga
    18901 tgatcgaaca aggtgcccct ggcggccaaa tgaacaatac ggcagaaatc gaaaattatc
    18961 ctggattcga ttcgatcatg ggccccgatc tagcttataa gatgtatgaa agtgtctcaa
    19021 gatttgggac agaaaatgct tatggaatcg ttcaagatat caaggatcac ggcagccata
    19081 aagaagtcat ctgtgaagat aaaagttacg aaggaaaaac agtcattatc gcaacaggat
    19141 gtatccaccg taaattaggc gtacctggcg aggaagaatt tgctggacga ggcgtttctt
    19201 actgtgcagt ctgtgatggt gcgtttttcc gtaacaaacg tctagtagtc atcgggggtg
    19261 gggactcagc agtagaagaa gccatctatt tgacacaatt tgcttctgaa gtagtgatcg
    19321 tccaccgtag agatgaatta cgtgcacaaa agatcatcca agaccgtgca tttgcaaatg
    19381 aaaaaatttc ctttatgtgg gatacagtcg ttgacgaaat cgttggaaac gaaatggtag
    19441 tgacaggtgt gaaagcacgt aatgtcaaaa ccggcaatga atttgattta gcagcagacg
    19501 gagtattcat ctatgttgga ctggatccat taactgcacc ttttagagaa gcaggtttga
    19561 cgaacgaagc tggctggatt ccaacagaca acgaaatgaa aacgaatatg ccaggcgttt
    19621 ttgccatcgg agatgttcgt gaaaaagatt tgcgacaaat cactacagct gttggtgacg
    19681 gcggtattgc tggacaacaa gtgtataaat acttggagtc tttagaagaa acaacagcat
    19741 aagtttaaaa agagtagaag aacgggttca attgttcttt tactcttttt tttcgcgtga
    19801 aatttactga atctgattta tgcgaacacg tccaacacct tttgataaaa gaatcctttc
    19861 ttgatttaag cgttttttgg tcctcatact ttcatatcta ataagtgaat gctatactta
    19921 ttaagaacca tatttcagaa agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac
    19981 aatgggtaca tgaagaaaat ttagaagaaa acttaaaaaa acaattaaat gatctgggtg
    20041 aagatcctga aaaacttgaa gatgcttttt atgcaccgtt ggaattcggt actgcaggta
    20101 tgcggggaat cttaggacct gggatcaatc gaatgaacat ctatacggta agacaagcaa
    20161 cagaaggact agcacgtttc atggatacac aaggtgaaga aacaaaacgc cgaggtgtcg
    20221 ctatcgctta tgactcacgc catcagtcac cagaatttgc aatggaagca gccaaaacac
    20281 tagcacatca caatatcccg tcttatgtat tcgaaagtct gcgtccaaca ccagagcttt
    20341 cttttgcagt tcgtcatttg aagacgtttg cgggaatcat ggtaaccgca tctcacaacc
    20401 cagcggctta caatggatat aaagtctacg gagaagatgg cggacaaatg cctcctgcag
    20461 acgcagatgc attgacgaaa tatgtccgtg aagtcagcaa tccgctaaaa gtcgaagtat
    20521 tatctgacga agaagctaaa catagcgggt tgatcacgat catcggagaa gaagtggacg
    20581 ctgcttatct agaagaaatc aaagcagtga cgattgatcg tgaattggtt gaaacaatgg
    20641 ggaaagattt gaaacttgtg tacacgccac ttcatggaac agggaaaatg ttgggcgaac
    20701 gtgcgttgaa acaagctgga ttcgaacaat tcgtactagt tcccgaacaa gctgtggcag
    20761 atccagattt cactacggtc aaatcgccaa atccagaaga acattcagct tttgaatatg
    20821 ctatacgact tggagaaaaa gaaggagctg acttgctgat tgcaacagat cctgatgcag
    20881 accgtttagg cgcagcggtt cgtttgccag atggttctta tgaagtattg actgggaacc
    20941 aaatcggagc attgtttgct caatatattt tggaagcaca tcaatcaaga ggcactttac
    21001 cagaaaacgc agcggtattg aaatcaatcg tttccagcga attgcctaca gcaattgcgg
    21061 aacactacgg tgctaccatg ttcaatgtgt tgacagggtt taaattcatt gctgaaaaaa
    21121 tccaacaatt tgaagaagac cattcacata cttttatgtt tggattcgaa gaaagttatg
    21181 gttatttagt taaacctttt gttcgcgata aagacgcgat ccaagcattg cttttattag
    21241 cagaagtagc agcttactat aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga
    21301 tttttgaaac ctatggctat ttcgcagaaa aaacgatttc tgtgacaatg agcggacaag
    21361 aaggttctgc gaaaatctct gctttgatgg aaaaattccg taaagaagca ccgacagaat
    21421 ttgccggagc aaaagttatt caaacagaag actttaaaga attgacgaga acagaccgtg
    21481 aaggaaacgt agaaaaacta acaacaccgc catccgacgt attgaagtat acattggaag
    21541 aaaatggctg gattgctatc cgcccttcag gaactgagcc gaaaatcaag ttttatattg
    21601 gtgtaaaagg aagttctaca gaagacgcag ccgaaaaagt agcctcttat gaagcgatta
    21661 tcaaaaagat gacagaagaa taaaaaactt gagactgatg gaaaaagtgg taaactttag
    21721 aagtcagtct tttttcaagt tggaggtgaa tggattgcca gacacgcaaa aatcaaaagt
    21781 agagaaagtc agtcaattat tcaaaatgtt gagtgatccc actcgtctta aaattctgct
    21841 atatctgaaa gacggggaac aaaatgtgac agcaatcacc caagcagtgg aaatggagca
    21901 gtctgctgta tcgcatcaac tacgtttatt aagagaaaat catgtcgtca agtctcatcg
    21961 cgagggaaaa gcgattttgt atagtcttga tgattctcat gtactggata ttttgaacca
    22021 gacactcaaa catgtggaac atcaataacg ataggtgcaa atagataaac actggagtgt
    22081 gacaacgaag aaatttcgtt gtcacactcc agtgtttttt aagaaaaaga caaaaatgat
    22141 caatcgcttt tttctgttgt ttcagttcca taataactat gatcttctag gtctaaagca
    22201 ttcaaaataa gcgaagccgt ttcttcaata gataaagatg ctacattgat cactacacat
    22261 cctagttttt cataaagatc attcgcaaaa tcgagttcag ctctaatttt ttcgatatcg
    22321 gaataagcag tatcagggtt taatccgtaa gagcgcatcc gttctttacg gatattattt
    22381 aaaatatctg ggttatttgt tagtcccacg atttttttag gatcgatctc ccaaagctgt
    22441 ttgggaatat gtgcttgtgg gatcaaaggt aaattggcaa cttttaaatt tttgttagct
    22501 aaaaacaagc tcaaaggcgt tttggaagtt cgggatacgc ctagcaaaac gacatctgct
    22561 tctaaaaaac cacgaggatc tttcccatca tcgtatttta cagcaaattc cattgctttg
    22621 atccgtttga aatagttctc attcaataaa tggagggcac caggttgccg aattggcttg
    22681 ataccagtta aacgttcaat ttctgatatc ggtggcgtca tcacatcaaa actaaataat
    22741 tgatgttctt cacaaaaaga acgtgttatc tcgatcagtt cttgattgat taatgtatgc
    22801 aggacaagtg ctttttcttc taatgcatct tgcaatgcct gccttaatgt tttttcatct
    22861 gtcacgaacg ttctgcggaa taggttgaac tcaacggtag gatactgagc catagtagct
    22921 tgcgctagtt ttgtcgcagt ttctccagca gagtcggaga tgacaaaaaa agtaacgatt
    22981 tctgtagaat cggaagtcat ggataaaacc tcacttgcat tttttttatg tttattatac
    23041 catactctag aatgcgtact tcgtaatcat tttgatttgg agggataagt catgaaaaaa
    23101 cagacattga tcgaacaatc attagaaaca ttgaaaaata acggttttaa atatactaaa
    23161 aaaagagaag cattgttaac ttatttagtg aagcgtaacc gctacgtatc tgctaaagaa
    23221 gtttttgact atatgaatca ggaattcaaa ggggtaagtt atgatacgat ttaccggaat
    23281 ctccatgatt ttgcagagat cgatttactg gaagaaaccg atttaaatgg agaaatgaag
    23341 tttcgttttc attgctgtca ggggaaaatc ggccatcacc atcattttat ctgtacagtg
    23401 tgcggaacaa cgaaagaaat ccatatgtgt ccgatgaatt atttcgaaga acagctaggt
    23461 ggatgcacga tcgaagggca tagattcgag attttcggaa gatgtgaaaa ttgtcaataa
    23521 ggatttaaat aaaataacat ttgataattt taaaaaaaag caagatttcc ttgacggtca
    23581 aaacagcatt agatataata tttacaggac agtcttgttt tttgagatat atttcaataa
    23641 caattacggt tactttttaa gctcggaggg agggaattaa catgtcaaaa acagtcgttc
    23701 gcaaaaacga atcacttgac gatgctcttc gtcgcttcaa acgttccgtg tcaaaagcgg
    23761 gtactttaca agaatctcgt aaacgtgaat tctacgaaaa accaagtgta agacgcaaga
    23821 aaaaatctga agcagctaga aaacgcaaaa aattctagtt ctgtgatgaa ttggagttgg
    23881 tagtgtgtca ctactaagta gattaaacga tgacatgaaa gcagcgatga aagcgaaaga
    23941 taaggaatct ttacaagtca tccgaatgat caagtcatct atacaaaacg agcagattaa
    24001 agaaggtcac gacttgacag aagaagaaga actaacggtg ttgtctcgtg aaatgaaaca
    24061 gcgacgtgat tctttgcatg agtttgaaga agcaggacgt gacgatttag ctgaaaaagt
    24121 aaaaagtgaa attgtaatag tcgaaaaata tatgccggaa caactttccg acgaagaaat
    24181 tcgacaactt gttcaagaag caatcactca aactggtgct tcatcaatga aagaatttgg
    24241 caaagttatg ggtgcaatta tgcctaaagt caaaggaaaa gcagatggaa accaagtgaa
    24301 tgcgattgtg aaagaactat tacaatctta attttatcat ttaaactgcg gacagtgatt
    24361 atccgcagtt ttttgtgttc gtaaaaaaag aactaaaaaa cagctagaag aaagaatctt
    24421 attctt
//
LOCUS       DACIRY010000034        24390 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.034, whole genome shotgun sequence.
ACCESSION   DACIRY010000034 DACIRY010000000
VERSION     DACIRY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.034"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IYE22_001170"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IYE22_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ9506391.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IYE22_001171"
     CDS             complement(1551..2972)
                     /locus_tag="IYE22_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9506392.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IYE22_001172"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IYE22_001172"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ9506393.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IYE22_001173"
     CDS             complement(5383..6639)
                     /locus_tag="IYE22_001173"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ9506394.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IYE22_001174"
     CDS             7219..8520
                     /locus_tag="IYE22_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ9506395.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="IYE22_001175"
     CDS             complement(8589..9188)
                     /locus_tag="IYE22_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9506396.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9442..10323
                     /locus_tag="IYE22_001176"
     CDS             9442..10323
                     /locus_tag="IYE22_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506397.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10336..11946
                     /locus_tag="IYE22_001177"
     CDS             10336..11946
                     /locus_tag="IYE22_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ9506398.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12023..12682)
                     /locus_tag="IYE22_001178"
     CDS             complement(12023..12682)
                     /locus_tag="IYE22_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ9506399.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12965..13630)
                     /locus_tag="IYE22_001179"
     CDS             complement(12965..13630)
                     /locus_tag="IYE22_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506400.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IYE22_001180"
     CDS             complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IYE22_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ9506401.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IYE22_001181"
     CDS             complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IYE22_001181"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ9506402.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IYE22_001182"
     CDS             complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IYE22_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ9506403.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15944..16264
                     /locus_tag="IYE22_001183"
     CDS             15944..16264
                     /locus_tag="IYE22_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ9506404.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16376..17245)
                     /locus_tag="IYE22_001184"
     CDS             complement(16376..17245)
                     /locus_tag="IYE22_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9506405.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17341..18645)
                     /locus_tag="IYE22_001185"
     CDS             complement(17341..18645)
                     /locus_tag="IYE22_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9506406.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVSFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18642..19151)
                     /locus_tag="IYE22_001186"
     CDS             complement(18642..19151)
                     /locus_tag="IYE22_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506407.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19326..21461)
                     /locus_tag="IYE22_001187"
     CDS             complement(19326..21461)
                     /locus_tag="IYE22_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9506408.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21544..23199)
                     /locus_tag="IYE22_001188"
     CDS             complement(21544..23199)
                     /locus_tag="IYE22_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ9506409.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23201..23515)
                     /locus_tag="IYE22_001189"
     CDS             complement(23201..23515)
                     /locus_tag="IYE22_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ9506410.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23685..23945
                     /locus_tag="IYE22_001190"
     CDS             23685..23945
                     /locus_tag="IYE22_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ9506411.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23959..24273
                     /locus_tag="IYE22_001191"
     CDS             23959..24273
                     /locus_tag="IYE22_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ9506412.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6948 a         5042 c         4402 g         7998 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcata tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt
     9361 ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt
     9421 agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc
     9481 tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt
     9541 ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt
     9601 ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta
     9661 tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact
     9721 ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa
     9781 gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa
     9841 ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta
     9901 tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt
     9961 gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc
    10021 gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga agaaggaaca
    10081 ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat
    10141 agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa
    10201 tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg
    10261 aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga
    10321 tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac
    10381 cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt
    10441 ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa
    10501 accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct
    10561 gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct
    10621 gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg
    10681 attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct
    10741 gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat gatttttttc
    10801 caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg
    10861 gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt
    10921 tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat
    10981 gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt gacctatcca
    11041 tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata
    11101 atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt
    11161 gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta
    11221 aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc
    11281 tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt
    11341 atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt agtcttgatt
    11401 tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga agaaacccac
    11461 ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta ttggaccaat
    11521 atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg tgcactaggg
    11581 ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat gtttcttaaa
    11641 gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg ctttctattc
    11701 ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc ctttgcactc
    11761 aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc tgtccctagc
    11821 tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac gttatcatgt
    11881 atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga cttactagaa
    11941 ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc aaaaatccaa
    12001 catttggagg tcattttttt atttacaagt cttctaactg ctctttatca atgatccaga
    12061 cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat tttctagata
    12121 aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt ggcaggaaaa
    12181 agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact cgttcttcta
    12241 ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc tcaacgttga
    12301 ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag aaatcttttt
    12361 gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac tcatttggaa
    12421 cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt aaaagctgtt
    12481 cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg atcactaatt
    12541 gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga tgtttgacaa
    12601 gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa
    12661 cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt ctaataaaga
    12721 aacataactt cccttattcc aactgataat tttcttttat catagcataa acgtcaagcg
    12781 aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt ttccttcttt
    12841 tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt gaataatcaa
    12901 aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc ttatttcgtg
    12961 ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt tcttgtaagg
    13021 tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat agtttaccca
    13081 tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt gtatcggaca
    13141 aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc catatgtaat
    13201 aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac tggttatacg
    13261 ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa tagatttggt
    13321 attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga gattcgcttc
    13381 ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt tcgattttag
    13441 gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga atctctttcc
    13501 atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct gttggatggc
    13561 tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa tctgattttc
    13621 caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa ttttgcgata
    13681 tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac agccaaacaa
    13741 aaaactgcga tgagcatcac cgcagtttta ttgaacttaa ggttgattga tcacatctaa
    13801 aagcgttgaa aataaaatgg cactagaagc agctcctggg tcgatatgtc cgattgcacg
    13861 ctctcctaga taggaagctc gtcctttttt agctaccaag actttggtgt gttcttttgc
    13921 ctggtcaatg atttcttttg ttaaattccc tgccttcaat gcctctgtga ctggcagcca
    13981 aacatctaac atcgttttat cgtcagcttc tgcctttcct cggctttgaa tacccgctgt
    14041 tccctgctcg atcacttcgc ctaatttttc ttgagaatca atagaatcga cctcttttgt
    14101 cgctttcatc atgttcatga atgcagatcc gtataacgga ccagaagccc caccgacttt
    14161 cgatatcatc gccatagaga atactttgaa tgtatcgctg attgtttcag gatgatcggc
    14221 ttgaaaagct tcttcataag ctgaaacccc tcttcccatg ttatttccat ggtctccatc
    14281 gccaatcggg gtatctagat cactgagata ttgtttgttc tgattgattg cctctgcaaa
    14341 ttgcgtgagc cattcttgga tttcttgaac ggttagtttc atattatcac ccttttatct
    14401 tgagattttt taccatgaaa tcgtctgtac attgctttct aaagaatctt tccattgtgt
    14461 ctccgtcaag tcgatcagtg ttagagaaag accttgcata tctagtgagg tcataaaatt
    14521 accgactttt cggaaaacta cattgacgcc tctttcttcc aacagagcta aaacatcatt
    14581 catgaacacg aactgttcca tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc
    14641 ttcttttggt aaattcgagt aatcatctaa aatcttagta acaagttcct ttgccagtac
    14701 tttagaaggc tgcatctttt ctcgacgata gcctggctca ccgtggatac caacaccata
    14761 ctcgatctca tcttctccta gttcgaatcc tggtttccct acttctggta cagttgctgc
    14821 tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc ttttctccca gttctttcaa
    14881 ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg attttgtgta caaggacagt
    14941 acctgcaact ccgcgttttc cagcagtata cgtactattt tcaacggcga tatcatcatc
    15001 tacgatcaca ctttctactt caatatcgtc catagcagct aattcctttg ccatatcgaa
    15061 gttcaatgca tcccctgtat aattcttaac gatcagcaaa actccttttc cactatcggc
    15121 ttccttaata gccgtctgaa tctgatctgg agttggagaa gtaaacacat caccaagcac
    15181 cgctgcactt aacatccctt cccctacaaa cccggcatgg gatggttcat gtccgcttcc
    15241 tccaccagaa accaaaccaa cttgttccgc tttctgattc ttggctacaa cacgggaaga
    15301 ttctacacga tgaacaagtt ctggatagct ttttactaat ccttccagca tttcttcgac
    15361 aatattgctt ggatcattca tgattttttt caatttattc ccatcctttc tttccggcat
    15421 tttgtgcttc tgttaagatt tgctgagtat catctgttat accggcagtg atggctgctg
    15481 caaaagctcc ttccactaaa ggcgcatcca ccagatgata acgttgctgc tgctcttctt
    15541 ccaacatgtc ttttgccagt tccgagttca ataccgcgct accgatatcc gcaaaaacaa
    15601 aataactagc tgcatcgctt gattcattga ctgcttctac gattttcatc gggtctgatc
    15661 cgatctcgcc atttattccg ccgagagaaa aaatttgcac ctcttcggat ttttgcatct
    15721 gctcaatcat ttctttaata ccatctgtta ctttttgact atgagaaata agcaaaatac
    15781 tttttttcac aagaatcttc ctttctttta catagttgct aacgattggt ttcgctttca
    15841 ttaatgaaca attcaagtat ataataaatt tctaattttg ggaaaatatg tgtagtgctt
    15901 cttgaagttt gttatacttt aattaaaaga gaggagtgat cgaatgatta caacaacaac
    15961 gaacagtgta gagggaaaag ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat
    16021 tactgggatc catgcattta aagacttgga ggcggggttg cgaaatatct ttggcggccg
    16081 ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa gaagcacttg ccgaaatggc
    16141 cgaacgtgca aaagcattag gagctgatgc gatcattggc gtaaaaatgg actatgaagt
    16201 attaggcagc gacaatggca tgctgatggt cacatgtagt ggaacagctg tctccacgag
    16261 ataagtaggg gacatctgtt ttaataacta aagagagatg ggacatgtag tggcactcaa
    16321 gaattagatt tttcagtcta acttcgaggt actctacctt cccatcctct cttttttatt
    16381 tctttttgcg gatgattccg taagctgctg cgactaacac acccgcccct gctaaggaat
    16441 aactgacaga agctgttttt tcatttgttt ttggcaaagt ctgacttgtt gatttggatg
    16501 gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga ggtcactgat ttttctcctg
    16561 ccgaatgcaa aatatctttt attcgttgga aactagctgc taagtcagct ttttttgctt
    16621 caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc gtggattgct tgactggccg
    16681 ctgtgatatc cgcttgagtt tgacttagct ttccagaagc tgtttgagca acctgattta
    16741 attgctgatt gaattctttt gcttgttgga tcttcccgtc aagatctgtt tggacagctt
    16801 ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg tgcacggtca tttcccactt
    16861 tatttttaag atcagctaaa tcttgatcga ttgctgcttg gtcttctttc aatttgtttt
    16921 ttgcagcttc taatgcttct tcgagttctt ttcttttatt ttccaagtct tgcaaatgat
    16981 cgatttggtc agaagaggta gaactattta ttgatgtatc ttctgaagat gaaatcgttt
    17041 ctcctgaatt atctgtcggt acattggaag tcatttctgt ttccgaggca ccgttttcag
    17101 caccagaaga gcttatggtt gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa
    17161 aagctgtctg tcccaataag aaaatactgg aaacagcaat tggccagatt aacggcttca
    17221 ttcttttttt catcactttt cccatcctta ctacttttta actaatttct tattataagc
    17281 aaaaatgttt ggaagaacca taaacacaac aggctgtttg tctaaaaaac ggtgcatctt
    17341 ttattttttt gtacgttccg ggcttttcca tgtcccgctt ccataaccta aaatcgtttt
    17401 gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc actatccaga acaaaagcat
    17461 atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta caatcagcga
    17521 agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt
    17581 aaaagtaata taaatctgtt cgtagtttcc ttgatataca taatgcccga ttaatccagc
    17641 aaaaagaact acggaaatac aaaagaatat tgaccagaca atactcaatg tttgatcaat
    17701 gaagatgatc gttcttccaa tatgtttaaa aggatggagc atcacttttt tgaaattcgt
    17761 cagccagact tctgttccac cttttgccca acgttttctg tgtcgatata gcatcttcaa
    17821 cgtcacaggt acttccatga aaaagataat ctcaggagca aaaacagata accagtcatt
    17881 taactgatga tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc
    17941 cacatcgatc aaggcttctt ttcgatacat cgtatttgct ccactgtacg cataaagacc
    18001 accgaaaaca gctgactgtg ttcgtttaat gatgcccacg atacttgaga attcaaccgt
    18061 ttgagatttt gcaatcaatt tggtacgatt ttggacatcc atatttgcag tgaccgcagc
    18121 aatatgtctt gcaccagggc gaatgaaata attcacatac ctaatcagtg catctggctc
    18181 cggtaccgtg tctgcatcat tgcttaaaat caatttccct tttgcaaagg caagtccgat
    18241 attaaacgcg tgggcttttc ctttattttt ttcgatccga accacacgaa gattagcgta
    18301 ttttttcatc agtctagcta atatttctgg tgtttggtcc gttgaaccat cgtctgtcac
    18361 taatacctca tagttgtgat aattcaattt cgtcatcaaa tactcgattg tatattcgat
    18421 cacaatttct tcattatggg ctggcaccat gatcgtgatg aacggttcga cactcaaggg
    18481 aacatctacc cattcttttt gcttatgttt gtataaaaac acataacacc agacaccaat
    18541 aaacgataca aatccgccta aaatcggata agaaacgaga aaaaaataca taatagacaa
    18601 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
    18661 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
    18721 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
    18781 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
    18841 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
    18901 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
    18961 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
    19021 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
    19081 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
    19141 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
    19201 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
    19261 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
    19321 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    19381 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    19441 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    19501 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    19561 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    19621 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    19681 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    19741 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    19801 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    19861 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    19921 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    19981 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    20041 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    20101 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
    20161 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
    20221 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
    20281 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
    20341 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
    20401 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
    20461 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
    20521 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
    20581 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
    20641 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
    20701 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
    20761 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
    20821 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
    20881 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
    20941 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
    21001 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
    21061 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
    21121 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
    21181 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
    21241 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
    21301 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
    21361 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
    21421 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
    21481 taaagtggtg gaaccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
    21541 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
    21601 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
    21661 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
    21721 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
    21781 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
    21841 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
    21901 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
    21961 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
    22021 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
    22081 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
    22141 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
    22201 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
    22261 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
    22321 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
    22381 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
    22441 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
    22501 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
    22561 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
    22621 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
    22681 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
    22741 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
    22801 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
    22861 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    22921 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    22981 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    23041 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    23101 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    23161 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    23221 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    23281 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    23341 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    23401 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    23461 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    23521 gcacctcatt tacattttta taaatacctc acggggtata ttaaacagga gctttattaa
    23581 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    23641 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    23701 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    23761 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    23821 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    23881 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    23941 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    24001 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    24061 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    24121 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    24181 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    24241 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    24301 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    24361 aggttgtgaa aacgctgtcc tgcatcaagt
//
LOCUS       DACIRY010000035        24345 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.035, whole genome shotgun sequence.
ACCESSION   DACIRY010000035 DACIRY010000000
VERSION     DACIRY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.035"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1497
                     /locus_tag="IYE22_001192"
     CDS             <1..1497
                     /locus_tag="IYE22_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ9506413.1"
                     /translation="IFVGLLTGIVVSLFRLSIEKLSDLVRSIYEMSREQPIYLIGLAI
                     CCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSMNWFSVLWKKFIGGVLSVGAGL
                     FLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSGASSGLAAAFNAPIAGLLFVLE
                     EIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYLGDLPSLPLKYYGSLVILGVLL
                     GILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLILPIGYLWPSTLGGGNQIVLAFG
                     HASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPILTLGALIGSFYGNIMVDFGMDP
                     IYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLNHLMPLGLAALVAYIVNDLLGG
                     NPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSLDGLMVRDFNWPKNMLLISIRR
                     GNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEAKSSITNLKQSF"
     gene            complement(1608..3539)
                     /locus_tag="IYE22_001193"
     CDS             complement(1608..3539)
                     /locus_tag="IYE22_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9506414.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3557..4471)
                     /gene="pfkB"
                     /locus_tag="IYE22_001194"
     CDS             complement(3557..4471)
                     /gene="pfkB"
                     /locus_tag="IYE22_001194"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ9506415.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(4468..5220)
                     /locus_tag="IYE22_001195"
     CDS             complement(4468..5220)
                     /locus_tag="IYE22_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9506416.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(5379..5588)
                     /locus_tag="IYE22_001196"
     CDS             complement(5379..5588)
                     /locus_tag="IYE22_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506417.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(5599..6927)
                     /locus_tag="IYE22_001197"
     CDS             complement(5599..6927)
                     /locus_tag="IYE22_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506418.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(7292..7879)
                     /locus_tag="IYE22_001198"
     CDS             complement(7292..7879)
                     /locus_tag="IYE22_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ9506419.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(7962..9212)
                     /gene="clpX"
                     /locus_tag="IYE22_001199"
     CDS             complement(7962..9212)
                     /gene="clpX"
                     /locus_tag="IYE22_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ9506420.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(9459..10748)
                     /locus_tag="IYE22_001200"
     CDS             complement(9459..10748)
                     /locus_tag="IYE22_001200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ9506421.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(10902..11834)
                     /locus_tag="IYE22_001201"
     CDS             complement(10902..11834)
                     /locus_tag="IYE22_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506422.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EAWTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(11991..12650)
                     /locus_tag="IYE22_001202"
     CDS             complement(11991..12650)
                     /locus_tag="IYE22_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ9506423.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(12653..13678)
                     /gene="sppA"
                     /locus_tag="IYE22_001203"
     CDS             complement(12653..13678)
                     /gene="sppA"
                     /locus_tag="IYE22_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ9506424.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(13691..13981)
                     /locus_tag="IYE22_001204"
     CDS             complement(13691..13981)
                     /locus_tag="IYE22_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506425.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14391..16196
                     /gene="glmS"
                     /locus_tag="IYE22_001205"
     CDS             14391..16196
                     /gene="glmS"
                     /locus_tag="IYE22_001205"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ9506426.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(16268..17623)
                     /locus_tag="IYE22_001206"
     CDS             complement(16268..17623)
                     /locus_tag="IYE22_001206"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ9506427.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(17647..18816)
                     /locus_tag="IYE22_001207"
     CDS             complement(17647..18816)
                     /locus_tag="IYE22_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506428.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(18813..19700)
                     /locus_tag="IYE22_001208"
     CDS             complement(18813..19700)
                     /locus_tag="IYE22_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ9506429.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(19889..23569)
                     /gene="nifJ"
                     /locus_tag="IYE22_001209"
     CDS             complement(19889..23569)
                     /gene="nifJ"
                     /locus_tag="IYE22_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ9506430.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            23767..24288
                     /locus_tag="IYE22_001210"
     CDS             23767..24288
                     /locus_tag="IYE22_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ9506431.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6758 a         5252 c         4368 g         7967 t
ORIGIN      
        1 atatttgttg gactgctgac aggaatcgtc gttagcctgt ttcgattatc tatcgaaaag
       61 ctaagcgatt tagttcgaag tatctatgaa atgagtcggg aacagccgat ttacttaatt
      121 ggcctagcta tttgctgcat ccttgcagca tttttcgtgg gttatctggt caaaaatgaa
      181 ccggatatca agggaagcgg gattccacaa gtagagggac agcttcgcgg ggaattatca
      241 atgaactggt tctctgtttt atggaaaaag ttcattggcg gtgtactttc agttggtgcg
      301 ggcctttttc taggtagaga aggaccttct atacagcttg gcgcatccat tggacaagga
      361 gctggacaat tgtttcggag tccatcttca gaagaaaaga ttttaatttc tagcggtgca
      421 agtagcggac ttgcagcagc attcaatgct cctatcgcag gattactctt tgttttagaa
      481 gaaatccatc actccttttc tccacttgtt tggctgacat catttgcttc agcaatcact
      541 gctaacttcg tctctcttta ctttttcggc ttgagacctg ttttatatct aggtgacctt
      601 ccttctttgc cgttgaaata ttatggttcc ttagtgatct taggagtact tctaggcatc
      661 ttaggtttta tttatcagaa ggtcttgctg gctttgccaa aattttacaa ccgattgcct
      721 ttaccagctt attttcacgg gttcgttcct ttcttgctga ttttgccaat tggttattta
      781 tggcctagta ctctaggcgg aggcaatcaa atcgttttag cttttggaca tgcttccttg
      841 ccaatcatta tgattatcgg tttgtttgtt cttcgattcg ttttttccat gatttcttac
      901 ggtggtggtc ttccaggggg gattttcctt ccgatattga cattgggggc tttaatcggt
      961 agcttttatg gaaacattat ggttgatttc gggatggatc caatctacat caaggatttt
     1021 gtcgttattg ccatggctgg ttatttcaca gcgattggaa aagcaccgct gacagcaatt
     1081 attttagtga cagaaatggt tggctctctt aatcatctga tgcctttagg attggctgct
     1141 cttgttgctt atatcgtcaa tgaccttctc ggcggtaatc caatttacga atcattattg
     1201 gaacgtctac ttggaaatac tcagaccgcc tcactaaaag gtgcaaggac aacttttgat
     1261 tttccagtaa cggcagagag ttctttagac ggattaatgg tacgagactt caattggcct
     1321 aaaaatatgc tgctaatctc tatacgcaga ggaaatcagg aaatcttgac tcacggtgat
     1381 acgatcatga atgtaggaga tattttggtt atcttgactg atgaagcaaa cttgccaaga
     1441 atcaagcagg aaatcgaggc taaatcttct atcacaaatt tgaagcaatc attttaacga
     1501 acatggaaaa actattttac aacaactatt catctacact acttgattct atatgattct
     1561 ataaaaaaag aagagactgg gaaaaaccag cctcttcttt tttatttcta tgcttctgct
     1621 gctgcaggag cttttctcaa taaaccataa atcacaccag atacgactgc tccgatagca
     1681 ataaacacca aatacatgac cgggtgactc agtaagaata caacgaaaat cccgccatga
     1741 ggagccagta atttgatccc gaaagctccg actaatccgc cagttaaagc agaaccaata
     1801 ataaaacttg gaatggcacg aatcgggtca gctgctgcaa atggaatcga tccttccgtg
     1861 acaaacgata gtcccatcac gatgttcgtc aaaccagcat cttgatctgt tttgctgaat
     1921 ttattcttaa acaaacgtgt agcaacgaaa attgccaatg gaggtaccat tcctccagcc
     1981 ataacagaag ccatcacgac acttccgcca gttgcaacag tcgtagctaa agttgctgta
     2041 ccaaacacat aagcggcttt gttgattgga ccgcctaaat ctgccgccat cattccagct
     2101 aataatgcac caagtaaagc agcattcgaa ccactcatac ttgtcaagaa accgttcaat
     2161 gcatcattga ttgctttcat agggacattg accagcaaca tcaagaaacc agtaatcagt
     2221 aagccaaaca ctggataaaa gaggatcgtt ttaattccat ccaatgattt tggtaagttc
     2281 ttgaataatt tacgtaaaaa tacgatgaca taaccagcta agaatccacc aatcagtgca
     2341 cctaagaatc ctgcaccgcc tgtattagct aatgcaccag cagcaaatcc aacgatcaaa
     2401 ccagggcggt caccgatact tgaagcaata aatcctgcta ataccggcag catgaaacca
     2461 aatgcagctt gtccgatttg gttgaaccag ctagcagctt gattgtaatt ccctagattt
     2521 gataattgat catgtggcac gccaataaat tggtcgatca tgaaagaaag cgcaatagcg
     2581 attcctccac caataacgaa aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa
     2641 attctttgtc cgatagtacc atccgcattt ccttcttctg aactgctttc ttttccatca
     2701 ccatggaata ctggtgcagt accactaagg gctagattga tcagctcttc tggtttacgg
     2761 ataccatcac tgacaggacg attgatcagt tcttttccgt caaagcgatt catctctact
     2821 tttttgtctg ctgcaacgat tacaccttct gcacgggcga tgtcttcagc agtcaatcgg
     2881 tttttgattc cttctgaacc gttcgtttcg actttgatct ccacacccat ttctttcgct
     2941 tttttcttca gagcatcttc tgccatgtaa gtgtgagcga tccctgttgg acaagctgta
     3001 acggcaacaa cgaattttct ttctggatga gcagaagctt ctgcagcttg tgtttcttgt
     3061 tctgcttttt cttctgcttc tttttgctct tcagctgttt ggaacaagtc ttggacttct
     3121 tctggtgtat ttgcttcttt taattttcct acgaaatctg catcaatcag caatcgagat
     3181 agtgctgcta atgcttgcaa atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa
     3241 aacagataag ttggctgtcc gtctaaggct tcataatcta ctcctttttg gctttttgcg
     3301 aataaaactg ttgcttcttt aactgcactg tttttcgcgt gaggcatggc gattccatca
     3361 ccaagacctg tagaagtttg tgcttcgcgg gctagaattc cttctttata tgtttcgata
     3421 tcggaaatcc ggccggcatc aaacattttt tggaccattt catcgattgc gcctttttta
     3481 tcagtcgctt gcaaatccat gatcatcgct tctttgacca ttaaatcctt gatattcatg
     3541 attatcctcc ttgaaactaa tcaatttttg tgatttcaac ttctggcagc aactcatcaa
     3601 tcaattcgcg agctgccaag tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc
     3661 atttaaaggc ttcgattgga tctttcgtac ggctgtaagc cccaataaag ccggcaatca
     3721 ttgaatcccc tgcacctact gaatttttca atgggcgaac taatacattc gttttataag
     3781 ttccttgttc cgtgaagagc aaagcaccgt ctccagccat tgagatcagc acattctgtg
     3841 ccccttcagc tagcaatttt ttaccatacg gaatgatttc ttctacagat tggaaggttg
     3901 tattgtaaag ctcagctaat tcgtgattgt ttggtttgat caacaatggt ttcttaacaa
     3961 gtgcattttt taggtcatca cctgttgtat caatcacgaa atctgcacct ttttctcgaa
     4021 tgatatcaat cagttcttga taaaatcctt ttcgcaggct agccggcgcg cttcctgaca
     4081 atacgacgat atctccttgt cctactgttc ctaatgcttg cttcaactca tcgatttctt
     4141 catctgaaat agatgggccc aaaccgttaa tttcagtttc agtatctgat tttagtttga
     4201 tattgattct tgtatcttct tttacggttg tgaacaaaga ctggatttgt tctttttcaa
     4261 gccacttcga aataaattcg ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag
     4321 aatcgatccg tttcaaaata cgggaaacat tgatcccttt tcctccaggt aacttgaagt
     4381 cttccttcat tcggttcaaa tcgcctagtt ttaacccatc tacgcgtacg atataatcaa
     4441 ttgatggatt aagtgttact gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc
     4501 acggaattca ttaacgaatt ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa
     4561 aggtgcaaca gaggtgaagg acaactggtt gaatttggta tgatcaatca atacaaatgt
     4621 ctcttcggaa tttttcatgg ctaaacgttt aacagctgcc tcttctgggt ccggagtcgt
     4681 gaatccttgt tcaagatggg caccgttcat tcccataaat gctttattaa aatgaaaatt
     4741 ttttagttgt tctatcgcat tagatcctaa aacagcatta gttgccagct ttatcgttcc
     4801 acctaaaata atggtagcaa gctccaaatc aactaaattt gccgcatgtt gaacggaatt
     4861 tgtcactact gtgatctgtt ttccttgcag aaaaggaatc atttctaacg tagtagagcc
     4921 agcgtctaaa taaatgacat cttcgtcatt aatggtttca gctgccgatt tggcgatttg
     4981 ctgtttttcg tgaacgtttt tgagtgattt ttcactcata ttctgctcaa aattcaataa
     5041 ttgctctttc ttagctccgc catgaactct ttctagaaac ccactattct ccagctcttg
     5101 taaatcacgt cttatcgttg attcagaagc attaaaaagg tttacaagat cttgtgattt
     5161 cacgattttc tgctgttcaa gcaagtctag gattttttgt ctacgttcct ctgtaagcat
     5221 tttcacgatc acctcaatac aaataatagc atattttttt tctactcgca atataaatca
     5281 gtcaaaaacg ttcataaaaa ttataaatca gtcaaaaaaa aataaaaaac cagtcaaaaa
     5341 gactggtttt ttattttttt gtctatcatc aagccgtttt atcgtttttt attttcgctt
     5401 cttgtttttt tcatataaac agaagcaggt tttctttcct tatgaagaag ataataataa
     5461 agtcccatcc cgccaaacat gaccaaaaaa ccgatgactg atcgaatagg aaaattagga
     5521 acaaaaaaga gacagacgga taataatgat gccggaacca ttattcgata atagagttgt
     5581 ttatccattt tcttcacctc atattttttg taaagtaatc aattgttcta attctcggta
     5641 atctaaaaat tgcacacgca gatgctgctc acctttcttg acatcgaatt ggatacttcg
     5701 ccgctttttc tttattcgtt tgaaggtaac atcagagagt ctatgtttga tttcctttaa
     5761 cggtaattct gacagggatt ctgaaggaac atagattctt aaggataaaa gatactcttg
     5821 atatttttca tctgttacct ctacttctag atattcccta atataataac aataagcttc
     5881 gattccatta tccaaagcaa cagtcggatc gataacttta tagcacatca tgcgcgagcg
     5941 attctcgtca acccaaggaa aatacgcctg agcatttagt gttccgcgaa cacgcctttc
     6001 tttataatct ccttgttgaa tatcataact ttttgttaca aaaaatgaag cattcttttt
     6061 aggaacaggt tgcatttgtg cattctgata ctggacagat tggatcgtat ggtcagtgta
     6121 aatgtattca acgaatggcg gtttcaataa accgacacca ttgtcccaag caagatgatc
     6181 aaacccaagc tggtccctaa aataggattg aaacatttga ttgtttggct tttgccagtt
     6241 aaaggggtct cgataaagct ggatacatat gacttcgttt tctaattcct tcactttttc
     6301 tacttgttgg atgtcgagaa aaatcggttt agggatgatt tgatgataat agcgactata
     6361 atacatttcc caacacgaag tgaaacataa accttggtaa aaaagatcgt atcctggaaa
     6421 ttggttgcaa tcgactacta cttccccgtt ttgttgtttc attttaggta aatgttcgat
     6481 ttcatccttt ttctcaaaca atagccgttg agagatctgt tttgtattgt gatagataaa
     6541 ctcgctataa gctcgcatat aggcgaacac cccatgagca atcatttttt tctgaatgaa
     6601 gtctcgaatc aaaaattgct tttctttgaa aacagagata gacaatacat tttttatcaa
     6661 taattgatta tcttccaagc gaaaactaca ttcgttctcg tcatttccta caactaacca
     6721 actttcgtct cttaccgatg ctagcattat tttttcttga agctttcgaa gatcatacgg
     6781 atccgcttgt tgtgtgaaag agcggataaa tgttggctgg atcaacagac gtgtcaaaat
     6841 agcccaatcg cttatttcct gttttttttc ttcttcattt aagtctgtga gctggatagt
     6901 catcaataat ttatcttcgc caatcatttt ctcacctctt taaaatcttt ttataattta
     6961 attataacca acgataaaga aaaactaaaa ctttatgtct tagaaaccaa tttggacgat
     7021 tacatgatag actaagtaaa aagaaacaaa gaggtgttta caatggggtt gaaatttgaa
     7081 cgtacagata atttagataa acttttcgaa tcatttgctg tcgatccgga aaaaacagtt
     7141 gcaccaaaag aagagatcga tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa
     7201 gacaagaaac aaaagtaagt taagctcaaa aaaagactta ccagccaaac ttttatgaaa
     7261 gagtttgact ggtaagtctt ttttattctt tttatgattc aattgcttgt tcgattgctg
     7321 cccacgcttc ttcttttccc tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc
     7381 gatcaaaatc caacgctttt ttgatttcgc tctcatgctt gttccattta ccacgaggga
     7441 tcttatctgc cttagtggca acaacaatta cagggatatc ataatacttc aaaaattgat
     7501 acatctggat atcttcttga gaaggcttat gacgcaaatc tactaaagaa acgaccgcac
     7561 gtagctggtc tctagtcgtt aaatatgtct cgatcatctt tccccatttt gctcgctcag
     7621 ttttggatac ttttgcatac ccatatcccg gaacatcgac aaaatgtaat gcgttctcga
     7681 tcaaataaaa attcaatgtc tgggtctttc ctggcttacc ggaagtacgg gctaaattct
     7741 ttcgatcaat caatgtgttg ataaaagaag atttccctac atttgatcgt cctgccagag
     7801 ctatttctgg caattgtgta tctggatatt gttttggtgc cactgcactt atgataattt
     7861 cagcttgatg gactttcatg gtctctctcc ttaacaaggt gatgaataaa gcaagcggct
     7921 gtcgcaaaag ccaaaatgac ttttacggca gcctcttttc atcaacccgc ttttttctct
     7981 gaatctttat agattactgt tggctcacca gacaattcag ctgcttcttt tgtgataatc
     8041 actttttcga tgttttcatt tgaagggata tcaaacatca catccatcat gatttcttca
     8101 atgatggaac gaagtcctcg agcaccggta ttgcgttcga ttgctttttt cgcaatggct
     8161 ctcaacgctt caggttcgaa ttcaagttcg gtattatcta gagaaagaag tttttgatat
     8221 tgtttgacta gcgcattttt cggctcagtc aagatccgta ccagatcatc tgttgtcaat
     8281 ttctctaaag ctgccataac aggtaatcgt ccgataaact cagggatcaa tccgaatttt
     8341 agtaaatctt caggaatgat atgctgcatg acactttcgt cttcagaaag tttttggttt
     8401 tgtgtaccaa atccaatcgt cttttcgccc atacgattct tcacaatcgt ttcgatgcca
     8461 tcaaatgcac cacctacgat gaacaatatg ttcgtcgtat caatctgaat aaattcttga
     8521 tgtggatgtt tacgtcctcc ctgaggtggc acgcttgcaa cagttccttc tagaattttc
     8581 agcaaggctt gttggacacc ttcgccggaa acgtcacgag taatagatac attttcgctt
     8641 ttacgcgcaa ttttatcgat ctcatcaata tagatgatcc ctttttctgc acgttccaca
     8701 ttgtaatcag cagattgaag caatttaagc aaaatgtttt ctacatcttc ccccacataa
     8761 ccagcttctg tcaaacttgt cgcatctgcg attgcaaaag gtacattcaa tgtttttgct
     8821 aatgtttgtg ctaaaaatgt tttaccagac cctgtaggtc cgatcaagca gatattgctt
     8881 ttttgcagtt caacatcatc tgtgttttct tgtgcttgct gattgacacg tttatagtga
     8941 ttgtatacgg caacagctaa cgagcgtttt gcgcgatctt gtccaatcac gtagttattc
     9001 aatacttcaa gaatttctag tggtttaggg acttcagtga attcgcgtac agcttcttca
     9061 taaaattctt catcaatgat ttctttacat aggtcgatac attcattaca gatgtagacc
     9121 cctggaccag ctacgatctt tttcacttct tcttgggttt tcccgcaaaa agaacagcgt
     9181 acggtctcat tgctacttgg attgtcatac atggtcttca ccccttaact tgcaaaaagt
     9241 atgcaagaaa ctatcaaaga ctttacagtc ttttactatc ataacatagt ttagtaaaaa
     9301 gaaaaagtgt ttaacaaagg atcaactgac ctataccttt gcatttttta ctcttaacaa
     9361 aaaataagga actgtgacaa aatcgtgtca cagtccctta tttcgaataa acaattttct
     9421 agaagtagct tcttcagaaa tgatttatta cttgatttct attctttcgc ttctgtcgct
     9481 gtacctgtga tcaattcgat tgcttttttc attgtcacat catgttccag catatcttct
     9541 gtcaacacgc gtttgatttg ttcgattggc atgttgtatg tttctgataa ttcttggatt
     9601 tctgcattga tttcttcttc tgttgcttga agattttcag cagctgcaat tgcttcgatc
     9661 acaagatttg ttcttgtgcg catttctgct tcaccttcga attgtttatg caagtcttct
     9721 tctgtagaac cagttaattg atagtacatt tcaggagaga taccttgacg ttgcatgttg
     9781 ttcaagaatt cgtccattga acgatgtact tcatcatgaa ccatcacgtg tggcaattca
     9841 acgatttctg cgttttcaac agccatacgg atcgctgctt catcttttgc atcgtccgct
     9901 gctttttctt ttgtttctac taattctttg cgatattttt ctttcaattc gtctaatgtt
     9961 gatacttcgt catcaacatc ttttgcgaat tcgtcatcta attcaggcac ttcttttgct
    10021 tttacttcat ggattgtcac tttgaagact gcttcttttc cagcaagatc ttctgcttga
    10081 taatcttctg ggaaagttac atttacatct agactttcac ctgatttatg accaactaat
    10141 tgatcttcaa aacctggaat gaatgagcct gaacctaatt caagtgagta attttcgcct
    10201 tttccgccat caaacgcttc gccatctaca aagccttcga agtcgattac tactgtatcg
    10261 ccattttcag ctgcagcatc ttctttgata actaattcag cttgtgcttc ttgttcacgt
    10321 ttgatacgtg catccacgtc agcatctgtt acttcacggt cttgtttttc aacaactaga
    10381 tctttatatt gtcctaattt tacttctggt ttcactgtta cttcagcagt gattacccag
    10441 tcttgacctt tttccatgct ttcaacatcg atttttggtt gagaaacagg atcgatgcca
    10501 gcttctttga cagcagcttc ataagcttct ggtaatacag cattcaatgc atcttcatat
    10561 aaagcttctt caccgtacat acggttgaat acttgacgag aaactttccc tttacggaat
    10621 ccaggtacgt tcaagctttt tttcacttta ttaaacgcag ttgttaatcc tttttggatt
    10681 tcgtcttgcg caatcgtaaa ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt
    10741 gcagtcattt aattccctcc gagtttagaa attcatttta tacatcaaaa acgtttcatg
    10801 cataccttta aatagtacac taaccctttc aaattgtaaa catattgaat ggatagaatc
    10861 cttgtttttc ataacatttt tatctttttc actgataaaa tctaatggaa gaagatcatt
    10921 tgctgcaaaa ttttttctct tttttttaaa aaatcttgtt tttcatcttc tgtaccttca
    10981 tacatcgttt ttcctgtcca cgcctctaca gtcagttgaa accagcgctt cgggtcttcg
    11041 atgtatgtat catagacagg ataaacaatt gcagattgca acgtgaattc ttcatttaac
    11101 acggaactaa gcagcgcatc ttcattaccg taacgatctg aaaatacaca taattgctgg
    11161 taacttgcct gctgctgcgg ctttggcaga tcttttggta tgactttgac taacttttct
    11221 tcaatcgtca aatagaaaac aggatggcat tcgttcaatt gcctaagtgt ctccagtaat
    11281 tttgcccttg ctaaaatatg gacctctggc gatacaataa gtttactcgc tgtttggata
    11341 aactcatgat aaggaagctg ttcgatctca tcaatagacg caagctgacg tgcaggttcc
    11401 aacgaaggaa gttcctccga tttattgtgg agtgccttga tcttctgcct ctccatttgt
    11461 ccggataatt cttctgcaaa ttggattttt tcaatgaccc gctgctcgaa agcttcataa
    11521 aaatctgtac ttgctatcaa ttttctagca tataaaaaat cttgtgtact gattaacaag
    11581 tcaaaataaa attcagcaaa ctcctcattt tgaagatact gtttttcatg caagagtgct
    11641 tgcttcaatg cttctttttt ctcgtccaac tcgtaaagac aaaaaacaag caggctatta
    11701 gcctcaaagc tttctttcaa ctcgtaagct cgttgaaata attcttttgc ctgctgatat
    11761 tgctggtttg ctagtgcttg ttgtgctact cgcattaaat tctcatattt ttctggaaaa
    11821 tcaatcggct tcacgcagtg tcctcctctt gctcatgttc agctcatgtt caatagtagc
    11881 aaaacaaaaa aagcatgaca attaaaaata actgaagcac cctctaaaag ctagatttta
    11941 gatctgactt ttagaaggtg ctttcaactg ttactgcttt ttattgctca tcagctgttg
    12001 acttcctggt tgaatgcttt gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa
    12061 tcgcctagat gttgcttttt ctgattaaag attgctggaa gataccctaa catcagcaaa
    12121 ggaaatttta gaatgaaccg gcaagcacct tctctaacta aaacggttga ccagctaagt
    12181 tcttcttcat caaagctgat gactcgaata ccaaagatca ttttcccaat cgtttgacca
    12241 tgattgaatt tcgtcaataa aataaaatac gctagataaa taagtaaggc tgtgaatccg
    12301 tagatactca gataagaatc tgaaaaatta agccaaccta gatttttggc aataccaagt
    12361 gttaaacgcg taatagctcc gatacacaaa agatcgacca agaaagcaaa cattcggata
    12421 aaaaaacctg catagaaata cttaggaaaa tcatttgctt ttggcggttc atttcttgtg
    12481 tccgtatact ttttccattt acgttgtttc tttttgattt cttcgggcgt caaaggctct
    12541 ctttcaaaag agctcagtac cttttgggaa aagttggagt caggtcctac agcaggctgc
    12601 tgttgcttat ctttttcttg ttccttattt tcatttgaaa gctctgacat gcttactcac
    12661 ctccgtaata atacattgct tttggtgatt ccgctgtccc tagattctca agaacggata
    12721 agatctgact agtctcagaa ggcttcaatc cctggaattc tgctagttta cttcctaacc
    12781 atgtactagc aaacccagat ggcgtggaag aatattctac tagttccgca tcttcaagct
    12841 gttgttcctt tcgcatagct tttaaagcat cttcaggaaa tcccaattca tcgactaaac
    12901 ctacttcttt tgcttggaca ccatcataaa tccttccatc agcaattttt ttgacttctt
    12961 cttcagattt attccgtccc tcgctgacga ttgctacaaa acgactatat gcgctgtcga
    13021 tatatgcttg aagcaccgct tcttcttctt ttgtctctgg tcgcgttcct gaacccatat
    13081 ctttcaacgc tccgctttta actgttgtat cttccacacc cagtttttct agaagtcctg
    13141 aataattcaa tcctgacatg atcacaccaa ttgagccggt cactgtttct tctgttgcaa
    13201 agattttatc tgcttgagca gaaatatagt agccaccgct agctgccatg tttttcatgc
    13261 tgacatacat tgggatatca tgttctttac gaatcgtatc caatttctta gcgatttccg
    13321 cactttcgta gacaccgcca ccaggagaat ttacttctaa aaagatacct ttgactgttg
    13381 gatcttcttc aatcgccttc agttgttcca taaaattttt atgattgtaa ccttcagaag
    13441 caaataaacc tgaagcccct cccgcagcaa tcgttccatc tactgataat tttacgattt
    13501 tttgcgtact gtctccctct tccaacacat ttgaagctag ttcttcatcc ccatataacc
    13561 aactgttcaa tccctcaatt tgatcgtttt cctgaggctt agtcaaacta gctgaaacta
    13621 acgaaaccac aaataatcct gcagcaatca gaactgctgc ccaacgtctc ttattcattc
    13681 tttctccttt ctaatacaag ttttttatga cgatcacagt ttctgactgc tcagaagtgt
    13741 cgaaccgctc tgctttttct tcccgcgcat acccggaaaa cgactctttt gtcaattcat
    13801 cttctaccgt aaactcacga gcttcagcaa cttccaaggc atcaggtgcg atttcttgga
    13861 tgctatccca gccagtatct aaaaatattt tcgtatcatc tgatacacca gctgatgctt
    13921 cgaatttgga caattgtttt tttaacattc ccagtgtcat gatctcaatt tttgaatcca
    13981 tttttactcc tcctctatcc gattactttc tattgtaccg aactcctgaa aaagatgata
    14041 gataaaaacc cattattttt tgacataaaa agagcatttg acaaagcatt tgatatatac
    14101 caatttattt tttattcttt ttttgttgac aagaacattc gcaaaaggtt atgattttaa
    14161 atgtttccag ataaaaaaca aaagaggagg atagttatac cttttaggat agcgccaggt
    14221 ctgtgtaaac agatgacgag gagagggttt atcgaaagat tcggcggatg gccctaggga
    14281 ctgcactcta cagaaaatcc acaaaaaata tctgcaaaga taaaacaaaa ggattttcct
    14341 gcagctggaa gcattcatac gaattcatta tagaaagtag gatgtttttt atgtgcggaa
    14401 ttgttggaat gattggatta aaaaatgtaa caccaggatt gattgacggt ctagaaaaat
    14461 tagaataccg tggttatgat tcagctggta tctttgtctc agatggcaca actgattatc
    14521 tggtcaaagc acaaggcaga attcaaaatt tgaaaaacaa aataaatatt gatactacag
    14581 gaaatatagg tattggtcat acacgctggg caactcacgg gcaaccttct gaagaaaacg
    14641 cccatccaca tacttcccaa agtggacgct tcgtgctcgt tcacaatggc gtaatcgaaa
    14701 actttgaaga acttaaaatt gcttatttag caaatgacca ctttattggt gagaccgata
    14761 ctgagattat cgctcatcta attgaaactt tcgcgaagga tactacaacg caagaggcgt
    14821 ttttaaaagc tttgcgggtc atcaaaggtt cttacgcttt cgctttgatt gatcggacag
    14881 cacctgatgt tatttacgtc gctaaaaata aaagtccact attaatcgga ctcggcgatg
    14941 gattcaatgt gattgccagc gatgcaatgg ctatgcttgc tcatacgaag gaattcgttg
    15001 agattgaaga tgaagagatg gttaccgtca catcagaaaa agttattatt caaaattttg
    15061 caggtgacat cgtagaacgt tcttcattcg aagcacaagt agattcttca gatatcgaga
    15121 aaggtaccta tcctttttat atgctgaaag aaatcgacga acagcctatt atcatgcgtc
    15181 gtatcgctca aaaatacgta acagaagaca atcgtgtctc tcttgatcaa aagctggtcg
    15241 atacattgtc tgatagcgat cgaatctata tcgttgcttg cggaactagt taccatgcgg
    15301 ggttagcagg taaacagact ctggaaaaat tgacacagat ccctgttgag gttcatttag
    15361 ccagtgaatt tggctataac acacctcttc tttcaaaaaa accattcttt attttcttga
    15421 gtcaaagtgg cgaaacagca gatagccgcc aagttctggt aaaaatcaac cgtctcggct
    15481 acccttcact tacgattaca aatgttgctg gttctactct ttcgcgggaa gcatccttta
    15541 cactgctatt gcatgctgga cccgaaatcg cagtagcatc aacgaaagct tatactgcac
    15601 aaatagctgt actaacttta ttagccaaag ctatcggtga taaaaaagaa tttgaaactt
    15661 ctcttgtatt cgatatcact catgaattga gtttagttgc taatgcgatg gaatcggtca
    15721 tcgcccaaaa agattattta gaagagttgg cagaagtata tctcagcgat acaagaaatg
    15781 ccttttacat tggacgtggc gctgactaca acgtttctct tgaagctgct ttgaaattga
    15841 aagaaatcag ttatatccag gcagaaggtt ttgccgctgg cgaattgaaa catggaacga
    15901 ttgctttgat cgaagaagga acaccagtga ttggaatcat cagtgaagaa gtgactggtg
    15961 cgcatactcg aggaaacctg aaagaagtcg aaagccgtgg cgctaaaaca ctagttattg
    16021 tctcagaggg attagaaaag gaaagtgacc agctggtgct accaactgtc cacccttatc
    16081 taactactct aaccacagtc gtaccgactc aattacttgc atattacgca acgctattac
    16141 ggggattcga cgttgacaaa ccacgtaatt tagcgaaatc agtgacagta gaataataaa
    16201 agaacaagga ccagtgtcag acatctgaca ctggtccttg ttcttttatt ttttatgaaa
    16261 atatctctta atccagtccg atttcttctt tgactactgc cgcgatttta tcgacatagt
    16321 aattaacttt ctcatcagta ggcgcttctg ccatcactcg aagtaatggt tctgtaccgg
    16381 aaggacgaac taggattctt ccttctgatc ccatttcagc ttctgcctct tcaatcactt
    16441 ttttgattgc cggaacatcc atcgcaccgt ttttatcact tactcggata ttcactaatt
    16501 tttgcggata gattgttacc tctgctgcaa gctctgataa tttcttacct gtctgtttca
    16561 tgatattcag taactgaatt cctgaaagca tcccatctcc agttgtgtta aaatctaaga
    16621 aaatcatgtg cccagactgc tcgccgccaa agttataatc gtttttgcgc atttcttcta
    16681 caacgtaacg atcaccaact tgtgtaacga catctttcaa accgattcct tccaccgctt
    16741 tgtggaatcc taagttactc atgacagtcg taacgatcgt atcttttttc aatcgatttt
    16801 tttctgccaa gtatttagca cagataaaca tgattttatc accgtcgaca atgtttccaa
    16861 gttcatccac cgcaataata cgatcgccat cgccatcgaa agctagtcca gcatctgctc
    16921 ctttttcaac gaccatctca gctaaacgtt ctggatgggt agagcctacg ccatcattga
    16981 tatttaaccc gtctggattt gtgcccatgg tataaaaatc tgtttcaaga tcggcaaaca
    17041 aacgattaac acttgttgaa gttgctccgt ttgctgcatc gacacaaatg gtcaaaccgg
    17101 aaaggtcacc gttgatcgtt tgtacaagga attgagaata tttcaataag ccttctggaa
    17161 attcttctac agttcccaat ccttcagctg atggacgagg caattcatcc acttcagcat
    17221 ctaataatgc ttcaatttct agctcttggt catctactag tttaaaacca tctgcaccga
    17281 aaaatttgat tccattatct tgtgctggat tatgagaagc agaaatcatg acccctgcgc
    17341 ttgctttttg caaacgtgtc agataagcaa cacctggtgt agagatcaca cctagctgga
    17401 acacttcgat acccaccgat aaaagtcctg aaatcagcgc ttcttctaac atttgacctg
    17461 agattcttgt atcccgccct actaacacgc gtgggcgttt tccttcttct tcatgctggc
    17521 ttaggacata acctccgcac cgtcccagct taaatgcaag ttctggcgta agttccttgt
    17581 tggcttcacc gcgtacgccg tctgttccaa aatatttacc catagttgta aaattactcc
    17641 tttaacttaa ttttctgagg aatttgttct gctcatttgt gttgattcgc ttgtttctga
    17701 tgtttcttct gtacttgtgg tcgagctgct tgtcccagat tccgaactgg aagaaatggt
    17761 tgttgtcgat ggttccgttc ctgaatcagc agatgaaata cttctactcg tacttgtatt
    17821 ttcttgtgtc cgactgctcg tcgaagcaga gctactgctt gacgtttgca acacgggtgt
    17881 aatctccaca ctaactgatt cgggcttgat acttaacccc tcttctactg gtatttcaat
    17941 tttacgagtt gttcgatgat tgatacctga gacattgatc ggtacagcaa tactgtcacc
    18001 gatcgatgca agcaaatcgc tagagccgct tacttctgct tccagttttg aaaaactgaa
    18061 atcatagctt tctaccccgg cagctggtgt tccttgctgt atcccataaa gtcgtaccga
    18121 cttgcttggc ttgatcgaat cagctctcac tgttacttgc actggatcag agacgatact
    18181 taatacttcc cctgctgcat tcagcgcctg tattgggacc tcactattaa tcccatcatc
    18241 tgtgaccatc gaagcgtcca catcagctac tactcgtttg atttctgcca atgttttgtc
    18301 acctgtagtg atagagactt ttttaggact aacggaagta tcatcaagtt catatccaga
    18361 tggtgtgaac gttgaaggaa tcaacgtttc cacatcgaaa tcttttatta cctttttctc
    18421 aaccgttact gtaatagtcg atggttcgat ctttgctgtg acagcactgc tcaaattttg
    18481 aatttttagc ggaacctcat gtgttccttc gcccagctta gtgatatctg ctgtgacgcg
    18541 gaacatccgt gtatcttcgt ttgcctctgc attcaactga actcggtttg cactggaaag
    18601 tttgacagtc actgcatttt catatccatg gatatagtag cgctttgaat cataagaaat
    18661 cttgattggc accccagtaa ctgtttgatt atacgcttca tttccagaaa gcgtatttgg
    18721 aacggactgg ccatttgcat tgaagtacag cgcaatactg aacagcaatg caataaaact
    18781 gtaaagaatg cttgtccgtt tctcttttga gatcatttgc gatcccctcc cttagtcata
    18841 ccgtctagga aagattgaat cacgtttttc ttgttttttg ttttctcttc agcaatcagt
    18901 tctctacgca aaattgccaa atattcttgc tgggataagc cagccatcag ttcattatct
    18961 aatgtaatgc tgacatcccc agtttcttca gagacaacga tggtaatcgc gtcgctgact
    19021 tcgcttatac cgacagctgc ccggtgtcga gtcccgaatt cttttggaat caacatactt
    19081 tctgatagag gaagataggc gcttgctact gcgatctttc cttcttttac aataactgca
    19141 ccgtcatgca gcggtgtatt aggaataaag atattgatca gcagctctcc agtgatatct
    19201 gcatctaatg caattcctgt ctcaatatac tcatctaatc cagtatgccg ttcgatagta
    19261 attagcgcac cgatttttcg cttggacata tattggatgg ctttgtcaaa agacaagatc
    19321 atcttctcat cttcctgctg ttcgcttttt gattgtttga aaagcgaact gcgtccaaga
    19381 tgttccagtc ctctccggat ttccggctga aaaatcacga tagcagctat caccccgtac
    19441 gtaatgactt ggttcatcag ccaagacaag gtgtgcagac caatgatttc cgctacgatt
    19501 cggataacaa taaaaatagc tacacctttc agaagctgaa tcgcttttgt gcctcgcagc
    19561 agttggatca gtttgtatac aagataccag acaaccagaa tatcaagtat gttcaccaaa
    19621 aaattcagag agaacaagtc accggaaaac atttgctgcc aatagtccaa attaaaaagt
    19681 tccccgattt gaaatgacat aaaaagcctc tcttctcttt tgagatttcc tctctagtat
    19741 atcatatgtt ttaattttag aggctgatct gctcgtattt ttcttgagaa aaacgcataa
    19801 ttttcaggaa aattgcaagt atgcgtttta tttttctctg caataaatat ttttaaactt
    19861 cagagaatta ttcattatat tttttctgtt atttttcctt tttttctttt gtagcttgtt
    19921 ccattttttt acgtcgttct gctctggcag ctcttctttc ttctgcttca aggtccgtcg
    19981 ttttttcttt tctagtctta acgggtacat cgatagattc ctctggttca agtcgtttct
    20041 tgattttttc taattctccc gtcagtgcag catagttgta aaaacgtgtt ttagcgtctt
    20101 ggatagtctt ttcaaataaa gcttttgcaa gatttggatt tgcgttctct agcgaggaaa
    20161 agcgaacttg tttcctcatg aaatcaggca tagcagagaa atcaggtttt ttgtagtcca
    20221 aagtcattgg gttcttgttt ttttcgacca ttgcagggtt atatcgatat aatgaccaat
    20281 agccagaaga aacagcttct tttgcttctt taagcgattc tttcattcca ccaagcaaac
    20341 catgtgtgat acaaggcgta taggcaatga ttaaagaagg tccaggatac ttttctgctt
    20401 cttcaaaagc ctttatcgtc tgcatttgat tggcacctga agcaatttgc gccacataaa
    20461 catttccata tgtcatcgcc atcattccta gatctttctt tgaagcatac tttccactag
    20521 ccgaaaattt agcgattgcc gaagctggtg ttgcttttga ggtttgaccg ccagtattgg
    20581 aatagacttc gttatccaaa acaagaatat tcacgtctgc tccgctggca agtacatgat
    20641 cgatcccgcc aaaaccaatg tcgtaagccc atccgtctcc accaatcatc cattggctgt
    20701 tttttacgaa tagatccttt ctcttgtata tatcctccag ttcagggaca attgatcgtt
    20761 cgctttcgat cgcagcttgc agttttgctg ctctttgctg cgttccttcg ctctcaaaca
    20821 tgtgtaccaa ccagtcttcc atcaattctc gtagttcttc tgatactttt ggcaatactt
    20881 ctttgattga acttgccaaa gcctcgcgac gagtctgtgc cgctagaagc ataccataac
    20941 caaattcagc attatcttcc aataaagaat ttgaccatgc aggcccttga ccacattcat
    21001 tggtcgtata aggagaagca ggagaagcac cgccccaaat agaagaacag cctgtcgcat
    21061 tcgcgatcat cattcgatca ccgaacattt gtgtcaataa ttttacataa ggtgtttcgc
    21121 cacagcctga gcaagcgccc gagaattcaa gaagcggttg attgaattgt gtagacaaga
    21181 cagtattctt tcctttgact ggatttgctt tttgtcttaa tgtcattgca aaagcccaat
    21241 tcacagcttg ttctttctgt tcttcataag gcttcatcac taaagctttt tcttttgcgg
    21301 gacaagcctc tacacacaga ccacaacctg tacagtcttc cactgaaact tggatacggt
    21361 attttacgcc gtctgcgcct cgcatttctc tcacgataaa tccttctggc gcttcttcca
    21421 tttcctcttc atctgctaaa aatggacgga tggcagcatg aggacaaaca aaggcacatt
    21481 cattacacat cgtacagcga tcactaatcc actcaggtac ttctaaagcg acgccccgct
    21541 tttccataga agccgttcct aaaggcattt cgccagttgt cattccattt gctacaagtg
    21601 taccaacaga gagttgattt ccttcctgtg cattcactgg ttcaagtatt tcttgtatat
    21661 aaccagtgtt ccttttattg attttttcag gcatctccaa cgttctccaa tggtctggga
    21721 tcgcgacttg atgaagcagt tcggtcgtcc gatcgattgc ttgtatgttt ttttctacga
    21781 tagccattga tttatgtcca tagcttttca gggcttcttc ttttagcagc ggaagcacct
    21841 catcaaaagg aatgatgtca gctaatttaa aaaaagccgt ttccattgcc gtattgatcc
    21901 ggcgtcccaa accgacttct tgggccaatc tcatggcatt gatcgtatag aatctaatgt
    21961 tattttcagc caagtaacgt tttaatttta agggcagatg cgtttctaat tgttcgtcgc
    22021 tccaaagagt attcaataaa aaaatgccac ctggcttcaa tcctttgacc aagtcatagc
    22081 tatgcaggta agctggtgtg tgacaggcaa caaggtccgc atgctcgaca agatacgctg
    22141 aacgtatcgg cgtatctcca aaacgaagat gagagacagt cagaccgcca gattttttcg
    22201 aatcataata aaaatagcct tgcgcatatt tatcggtgtg atctccaata atcttgatag
    22261 ctgatttatt tgctccaact gttccgtcag atccaaatcc ccaaaacttg gcctggaatg
    22321 tttgcggttc tgtcaaatcc agactttcct ttggttccaa agattgataa gtcacatcat
    22381 ccacgatccc tatggtaaac cgttttttgc gtatgcttgg gtcttttttc aattcatcaa
    22441 aaactgctga gatttggtcg ggagtcacat ctttagaccc caatccgtag cgaccaccaa
    22501 taacagccgg gcgaagttca gaatcatata acgcactttg aacgtctaat aagagaggtt
    22561 ctccacctgc ccctggttct ttacttcgat caagaacagc aattgttttt actgtatgag
    22621 gcaacttttg cacgaatgtt tccactggaa aaggacgata aagatggata ttcaaaaaac
    22681 ctgttttccg tccgtttgca ttcaagtaat caatcgtttg ttcaatcgtc tgtgcaactg
    22741 atcccataga aacaatgact tcttccgcat cgtctgctcc gtaataagtc accaagtcat
    22801 aagaagttcc tcgtaatcgg ttgatctctt tcatatattt ttgaacgatt tcaggtaatt
    22861 tatcataata ggggttgatc gtttcccgtt gttgaaaatg gatgtctgga ttctggttcg
    22921 taccactaac agtcggatga ttgggattca tccctcgttt acgaaatgaa gctaatttct
    22981 cctgattgat cagaggagct aattcttcat attccaaaac ttcgattttt tgaatctcgt
    23041 gactcgtacg gaatccatca aagaaattca aaaaaggtac ttctccttca atagaagcaa
    23101 gatgggcaac aggtgctaaa tccatcactt cttgtacact gctttcagct aacatagcaa
    23161 atcctgtttg tcttgcagcc atcacgtctc cgtgatctcc gaaaatattc aacgcatttg
    23221 tcgtcactgc ccgactagca acatggaaaa cacttggcag tagttcccca gcaatcttgt
    23281 acatattcgg tatcatcaaa agcagccctt gagaagcggt atatgtagtt gtcagtgcac
    23341 cagttttcaa tgatccatgg accacaccag ctgctcctgc ttcggattgc atttctacta
    23401 cttttactgg ttgaccgaat acattttctt ttcctgctgc tgaccattga tctactaatt
    23461 cagccatggt cgaactgggt gttattggat atattgcagc aatctcggta aatgcataag
    23521 aaatatatgc ggctgccgta ttgccatcca ttgttttcat ttttcgcatg attcatcatc
    23581 cttgttcata ttctctctta ttatctgttc cattataacg taaaacattc cattttttct
    23641 tttatttata aaaatatttc acatatgtct atttgaattg gtcatctaat ttttagacaa
    23701 acagtctaaa tcgttgcctt tttttgtcag acatgtataa actataagcg aaagaggtgt
    23761 tttaacatgg atatatcagt gattgatgca acgaaaacaa atacacaaaa aggaatcctt
    23821 tatggttcat cgaatgctcc taaaaaaatg gtcgaattca tcaacctatc ttgtccgtat
    23881 tgccgacaat ggtttgaaga atcttatgag ctattagaag aagcagtcca aagcgggcaa
    23941 ttgcagcgag tgatcaaatt gttcgataaa gaaaaagaaa gcttgcagcg aggaaacgtg
    24001 atgcaccggt atctcacaat cagcgatggt caaaaagcga tcaaagagat caaacaaata
    24061 ttcgatactc aagacgagtg gaaacatctt tctctccaag aagtagcgga ttttgcagta
    24121 gataaactaa agctgacaga acaaaaagac gaacagctga gccaagctgt catcaacgaa
    24181 gctgagcaag cacatattcg atttgttccg actgtcatct tgggtaaaga aatctttgat
    24241 gaatctattt caatagagga attaaaagaa ttgatccaaa caaaataaaa aaataaggag
    24301 cgtaggacaa aagtaaagaa cacttttgtc tcacgctcta aacac
//
LOCUS       DACIRY010000036        23829 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.036, whole genome shotgun sequence.
ACCESSION   DACIRY010000036 DACIRY010000000
VERSION     DACIRY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.036"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(53..787)
                     /locus_tag="IYE22_001211"
     CDS             complement(53..787)
                     /locus_tag="IYE22_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ9506432.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1526
                     /locus_tag="IYE22_001212"
                     /pseudo
     CDS             1114..1526
                     /locus_tag="IYE22_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
     gene            1519..2220
                     /locus_tag="IYE22_001213"
     CDS             1519..2220
                     /locus_tag="IYE22_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ9506433.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IYE22_001214"
     CDS             2220..3806
                     /locus_tag="IYE22_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ9506434.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISPMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IYE22_001215"
     CDS             3825..4046
                     /locus_tag="IYE22_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ9506435.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IYE22_001216"
     CDS             4043..4804
                     /locus_tag="IYE22_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ9506436.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IYE22_001217"
     CDS             5184..5693
                     /locus_tag="IYE22_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ9506437.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IYE22_001218"
     CDS             complement(5768..6307)
                     /locus_tag="IYE22_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ9506438.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IYE22_001219"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IYE22_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ9506439.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IYE22_001220"
     CDS             7374..8171
                     /locus_tag="IYE22_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ9506440.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IYE22_001221"
     CDS             8199..8834
                     /locus_tag="IYE22_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ9506441.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IYE22_001222"
     CDS             8854..9627
                     /locus_tag="IYE22_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9506442.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IYE22_001223"
     CDS             10035..10832
                     /locus_tag="IYE22_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ9506443.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IYE22_001224"
     CDS             10810..11223
                     /locus_tag="IYE22_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506444.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IYE22_001225"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IYE22_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ9506445.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IYE22_001226"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IYE22_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ9506446.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IYE22_001227"
     CDS             16000..16560
                     /locus_tag="IYE22_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ9506447.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IYE22_001228"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IYE22_001228"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ9506448.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IYE22_001229"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IYE22_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ9506449.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IYE22_001230"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IYE22_001230"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ9506450.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IYE22_001231"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IYE22_001231"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ9506451.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IYE22_001232"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IYE22_001232"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ9506452.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IYE22_001233"
     CDS             21907..22485
                     /locus_tag="IYE22_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ9506453.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IYE22_001234"
     CDS             22537..23019
                     /locus_tag="IYE22_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ9506454.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IYE22_001235"
     CDS             23136..23510
                     /locus_tag="IYE22_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506455.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7780 a         4286 c         5117 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct ccaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagtt
//
LOCUS       DACIRY010000037        23541 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.037, whole genome shotgun sequence.
ACCESSION   DACIRY010000037 DACIRY010000000
VERSION     DACIRY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.037"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            145..1008
                     /locus_tag="IYE22_001236"
     CDS             145..1008
                     /locus_tag="IYE22_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9506456.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQAIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1137..2759)
                     /locus_tag="IYE22_001237"
     CDS             complement(1137..2759)
                     /locus_tag="IYE22_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9506457.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3008..3529
                     /locus_tag="IYE22_001238"
     CDS             3008..3529
                     /locus_tag="IYE22_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506458.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3715..5445)
                     /gene="spxB"
                     /locus_tag="IYE22_001239"
     CDS             complement(3715..5445)
                     /gene="spxB"
                     /locus_tag="IYE22_001239"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ9506459.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5610..6428
                     /locus_tag="IYE22_001240"
     CDS             5610..6428
                     /locus_tag="IYE22_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ9506460.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6531..6728)
                     /locus_tag="IYE22_001241"
     CDS             complement(6531..6728)
                     /locus_tag="IYE22_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506461.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6897..7475
                     /locus_tag="IYE22_001242"
     CDS             6897..7475
                     /locus_tag="IYE22_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ9506462.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8521..9024)
                     /locus_tag="IYE22_001243"
     CDS             complement(8521..9024)
                     /locus_tag="IYE22_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ9506463.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9383..10408
                     /locus_tag="IYE22_001244"
     CDS             9383..10408
                     /locus_tag="IYE22_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ9506464.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10755..11633
                     /locus_tag="IYE22_001245"
     CDS             10755..11633
                     /locus_tag="IYE22_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506465.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12109..12225
                     /locus_tag="IYE22_001246"
     CDS             12109..12225
                     /locus_tag="IYE22_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ9506466.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13153..13491
                     /locus_tag="IYE22_001247"
     CDS             13153..13491
                     /locus_tag="IYE22_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506467.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13585..13815
                     /locus_tag="IYE22_001248"
     CDS             13585..13815
                     /locus_tag="IYE22_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506468.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14215..14586
                     /locus_tag="IYE22_001249"
     CDS             14215..14586
                     /locus_tag="IYE22_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ9506469.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14860..15552)
                     /locus_tag="IYE22_001250"
     CDS             complement(14860..15552)
                     /locus_tag="IYE22_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ9506470.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15555..16340)
                     /locus_tag="IYE22_001251"
     CDS             complement(15555..16340)
                     /locus_tag="IYE22_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9506471.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16577..17977
                     /gene="lacG"
                     /locus_tag="IYE22_001252"
     CDS             16577..17977
                     /gene="lacG"
                     /locus_tag="IYE22_001252"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ9506472.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18002..18319
                     /locus_tag="IYE22_001253"
     CDS             18002..18319
                     /locus_tag="IYE22_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ9506473.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18332..20005
                     /locus_tag="IYE22_001254"
     CDS             18332..20005
                     /locus_tag="IYE22_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9506474.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20019..21650
                     /locus_tag="IYE22_001255"
     CDS             20019..21650
                     /locus_tag="IYE22_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ9506475.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22522..>23541
                     /locus_tag="IYE22_001256"
     CDS             22522..>23541
                     /locus_tag="IYE22_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9506476.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYSPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQI"
BASE COUNT         7344 a         3974 c         4763 g         7460 t
ORIGIN      
        1 tatttcttga agctgaccac ttctgtcaca acctcttatt ttgattagaa cttgcgaaaa
       61 taaaccggtt aaagtgtgtc tagcgcaaga gatttgctat aatagacgat aagggtaaaa
      121 tgtaacgaga aggggttgaa aaatgtgtat caaattgtaa cagattcttg ttgtgatctg
      181 ccataccatg tattagcaaa agaaaaagtt gattttttat caatgaatct ccaattgaat
      241 gatcaagagt tagtagatga cctcggaaag acattcaatt atgaccgcta catgaatcag
      301 ctgaaaaatg gtgcgatgcc tacgacatca caaatcaatg ttggccgata tttagaattt
      361 ttccgttctt atgcggaaaa acagattcct gttttatatg tggctttttc cagtggatta
      421 agcggctctt acagtagtgc gcttcaagcg gttgaattgc tgaaggaaga atatgaagac
      481 cctgagatct atgtttttga tacaaaagct gcaagtattg gggaagggct tttagtattg
      541 gaagctgtaa gattgaaaaa agaaggtaaa agcatcgaag aaaccctaga gtggctggaa
      601 agtaattatt tgaaagtcca ttcatgggta acagtagacg atctgaaata tctagaacga
      661 ggcggtagga tttccaaagc aagtgctact cttggcagtc tattgagtgt aaagcctatt
      721 attacggtgg ataaagaagg aaggctgacg aatgttgaca aagttcgagg aagaaacaag
      781 tcgatccagg caatcgtcaa tgagacggta aaaacaatcg ttgcaccact tgatcaaatc
      841 gtttatgtgg cttacgctgg ggatctagag agcgcggaga aggcaaaacg gcttttggaa
      901 gagcagataa agatgaaaga tgtgaagctt tatcccttag gaccaacgat cgccagtcac
      961 acaggctatg gctgtatcgc gattttttcg atgggaatca gtcgataaaa gcagtaactc
     1021 attctgtaaa aaagcccaga aattgattgt aacaaaaaca gcgactctta aaagatagat
     1081 gagcatccta tctcttagag gtcgctgttt ttttattttt taagctaaat aggccattat
     1141 gcccataact cgttcaattg tttttgaata tctgcatttt ccaaaaattc atcatatgtc
     1201 gtatcagctc gatcgatcac acctttatcc gaaacggcaa tgatgcggtt tgccaatgtt
     1261 tggataaatt gatggtcatg tgacgcaaat aagattgagc cagtaaatgc catcaagccg
     1321 tcattcaatg ctgtgattga ttctaagtct aaatggttcg ttggatcatc taatacaagt
     1381 acgttagctt tcgaaagcat catttttgaa agcatgcaac ggactttctc tccaccagat
     1441 aaaacattca ctggtttcaa tacttcttca ccagaaaaca acattcttcc taagaagctt
     1501 cttaagaagg tgttatcatc ttcttcttta cttgcatatt gacgcaacca gtctaaaatc
     1561 gttagatcag tatcgaattc tttcgttgta tcctttggta aataagaatt acttgttgtc
     1621 acaccccaac gtactgttcc agtatctggc gtaatttcac ccatgataac tttgaatagc
     1681 gttgttgttg ttatatcatt ttctgcgacg aaagcgacct tgtcttcttt gctcaaatta
     1741 aaagatatat catctaaaat tttctttcca tcaatcgtta cacttacatt ttctacctgc
     1801 aataagtcgt ttccaatctc acgttcaggt ttgaatccaa cgaatggata acgtctagaa
     1861 gaaggttgga tatcgtctaa agtaattttt tcaagcattt ttttacgtga agttgcttgt
     1921 tttgattttg atgcattcgc actaaagcgg gcaataaatt cttggagttc tttgatttgt
     1981 tcttcttttt tggcatttgc gtttgcttga agtttagccg ctagttgact agattccaac
     2041 caaaaatcat aatttcctac ataaagtttg attttaccaa aatctagatc cgccatatgt
     2101 gtacatactt tgttcaaaaa gtgacggtca tgggatacaa cgatcacagt attttcaaaa
     2161 ttgatcaaaa attcttccag ccaattgatc gattggatat ccagaccgtt tgttggttcg
     2221 tctagcaaaa gaacatcagg tttaccaaat agtgtttgtg ctaataacac ttttaccttt
     2281 tgtccaccag ttaattcact catttgctga tggtgcaatt cttcagggat gttcagacct
     2341 tgcagtaaaa cagctgcttc tggttcagct tcccatccat taagctcagc aaattctcct
     2401 tctaattcag ctgctcgaat accatcttcg tcagtgaaat cctctttcat gtaaatcgca
     2461 tctttttctt tcagcacttc atacagacgc ttgtgtccca tgatgactgt ttcaagtaca
     2521 gtatagtctt catagtcgta atggttctgt ttcaatacgg ccatccgttc atctggtccc
     2581 atagtaacgg aacctgtgga aggctgcaga tcccctgata acactttaag gaacgttgat
     2641 ttacctgcac catttgcacc aatcaagcca tagcagtttc cgggggtaaa tttaatattc
     2701 acatcatcaa atagtttgcg gtctgaaaag tgcaaactca catcatttac agtaatcaat
     2761 ttttttcctc acttttatca ttattagttt tgacaagtct tatagaatta tataggggaa
     2821 aggtaaaaga ttcaaggcaa tcgaacattc tttgtgtttc acgtgaaaca gaatgaaaaa
     2881 aataaaaata aaagcggagc gaaaatagct actataggat ttattagttt gcaacagcat
     2941 aatttttgac gttatttata agagggagta ctataaaact gtaaaagaaa tgaaagaagg
     3001 attgaaaatg gatgaaaaaa gaaccatcga ctggggctca ttgattttgg gaattttatt
     3061 tgtacttgtc tcattgttat catttcaaga ccctgtcgga aatttagttg caattgtagt
     3121 agtctttgcg atttttgcct ttattaaagg gatatttgaa ctgtttgtac ggaacagatt
     3181 aaaagagcta acaggatata aaggaaagat gcccttaata atcgggatca tcgatattct
     3241 agttggcgtc tttttcttat tcaatattgg tgcaggagtc gcagcattgc cttttgtttt
     3301 tgcagtatgg tttattgctg attcagtttt agcgttattt actgctgact tggcaagagg
     3361 agtaagtgaa ggatattatt ggtttacgat catcgtcaat attttaggaa ttttattagg
     3421 aattttctta ttgtttaatc caatttcttc tgcgttgaca ttaagctttt tagttggatt
     3481 ctattttatg ttatttggta tcacacatat cgtctatgca tttagataag taaaatcaaa
     3541 caaataaaaa agccggcttc cgattacagt tttactgttg taattggaag ccggcttttt
     3601 tatcatggac aaatatgcac gaggtgattt ttaggtgtcg agacaaattt atgcaaaaat
     3661 gtgagaagct atatacacaa attttatctt aatactcgaa gcatagcgca gttgtcacaa
     3721 cctcttttca tataattcat tcaatactgg caaatcatgg acttcatatt tttctttgaa
     3781 tgcttggatt tcttcttcag aatattttct tggatctaaa tgcagatctt ctactggcaa
     3841 tgggcgcttg ttattgatct tcacatcgat tactacagga cgatctgatt ttgcagctgc
     3901 atcaaaggca aatcgaagtt gcgagtattc tgtgactgta aagctatttg ccccgagcgc
     3961 ttctccaact ttaccgaaat ctgcatcttc taaaaataca ccaaatttct tttgttccgt
     4021 atcttcttgt tctgcttcga tgaaaccaaa agaatcattc gaaaagacaa cattaataat
     4081 tggaagctga tatttgactt gtgtaatgat atcttgcatg accatagcaa acccgccatc
     4141 accacttaag gtaaatactt gtttttctgg gtagcttagt tgtgcggcaa ttcctcctgg
     4201 cacaccatta cccatagttg cgaaccatcc agatgtcgta tgtttttgtt taccgttcat
     4261 attcaataaa cgaatagaat ggatagtcgt gttaccgaca tctgtgacga aaatcgcatc
     4321 gtcttcagca atacgattga tttctttata gacagcctct ggacgaatcg gttcttcctc
     4381 tcgatcttca aaagatttca accaatttac ccatttttct ttattttctt gatttgctag
     4441 gtaccagcta tcagcagggc gagcatcccc taactcgaca agtctttgca aagctgtgtt
     4501 tgcatcaccg aggatcgaca aagatgtttc atggcgtcgt ccaaattttg acgcatcgat
     4561 atcgatttgg ataaattcag cagttggatt aaaaaatgca cgtccaaatg ggaagtcgct
     4621 accgacaaat aagatcagat cggcttctgc caatgcttca tttccgggtt tagtagcgac
     4681 acgtccagca aaaccaagaa agttttcata atcatcagga ataattcctt ttgctagaac
     4741 ggaagcgaca acaggaactg aaaaatgttc tgagaattct ttgatttgcg gaaatccgtt
     4801 tcgcgttcct tgaccaacat aaagaactgg tttttttgct ttttcaagat aaggcaaagc
     4861 agctagcaaa tctttttctt ctggtaatat cacacttgtt ttgtgagtgt gagcggtgga
     4921 gaaaggttgt tcgtcaattt cttcaaaacc aagatcaaca gggatagtca cgatagccac
     4981 gcctttatgc tcataggcgg ctttaatggc ttcatctact acatgcggaa gactttgtgg
     5041 tgtcatgact gtacgattgt atacgctaac atctgcgaaa ataggatttt catttagttc
     5101 ctggaaagag ttatagttca tggatgtaga agcaacttgt cctaagaacg ctactagagg
     5161 cacatggtcc atctgtgcat catacaaacc attgatcaaa tgtgtcgcac caggtcctgc
     5221 agagccgaag acagcgccga ctttccccgt taattttgca tctgcagcgg ctgcgagtgc
     5281 gccgacttct tcatgacgaa cttgaatgta gttgatttcg gttttttctt gatatagcgc
     5341 atccataatt gaattgaaag atcctccggg aatgccgtaa atatggtcga taccccagct
     5401 ttcaagaact tttaccattg cgattcctgc attgattttt gacatggtgt ttcactcctt
     5461 tagcaatgat gatacataaa ttttttgtat ctagagtata gaacttttta tcgaaaatcc
     5521 caactaattc gtcttttcac aaaaatgtag tcatgttttt gggaatatgc tagagtagta
     5581 catagataga ttcaagagag gggaatggca tggttgaatg gctattgaga cttgaacctt
     5641 ggcagcaagc acttgctggg acagctttta cttattttat gacagctctt ggtgccgggt
     5701 tggttttttt ctttaaagaa atcaaaaaag aagtgctaaa cttgatgtta ggatttgctt
     5761 ctggtgtcat gattgctgca agtttttggt ctttgttaga tccggctatt acaaaagcag
     5821 aagaaaatgg agatattgct tggctagttg taagtattgg gtttggttta ggcggtgtgt
     5881 ttttatatat ggcagataaa acactgcccc atatgcattt tggaccacaa catgaagcag
     5941 aaggattgcc tacccattta aaacggacga tcttgcttgt tttttctatt acattgcata
     6001 acattcctga aggtttagcc gtgggggtag cattcggtgc agcagcaact gcggataatc
     6061 cgacagccgc tatattagcc gctgtttcgg tggcgctagg tattggtatc caaaacttcc
     6121 cagaaggagc agctgtatca atcccgttgc gacaagaagg tctgagtcgt aaaaaggcct
     6181 ttgtttacgg acaagcttct ggaatcgttg aaccaattgc tggagtgatt ggagcgctac
     6241 tagtaacgaa agtggagctg ctgcttcctt atgcacttgc ttttgccgca ggagcaatga
     6301 tttatgtcgt ggtggaagaa ttgatccctg aagcacagca gacattgtcc agcaaacgtc
     6361 attttgcagt ttttggcgtc atgagtggat ttatcattat gatgattttg gatgtggcat
     6421 taggataaat aaaaaaaata tatatgatat atcaaatagg gccgcgaact tatatcgcga
     6481 ccctattttt ttagtaagtt atatcataat gaagtgaatg gtatcataca ttaataatag
     6541 ttttttctat gtgtatttcc tttgatccag cgaacaagcc agatgataag tgcgattggg
     6601 attagaactt tgaccgcaaa ccataaaagc ccgcttagga cactgacaaa aagactgatg
     6661 acgaagctag ctgcaattag cactaaaaga accatgaaga tatttaagaa catatttttt
     6721 tgacgcatac aaaaccctcc tcttgttcat tctttactat agaaaaagta taacagggat
     6781 tgtttaaaaa cacctccaac tgaagactga tttttacttc atctacttaa atgatcaaac
     6841 aattgctttt tctctggttt atcatctaaa atagagcgga aaaggaggag aatatcatgc
     6901 ctaaaaatcg tcttgaagca tttacagatg ctgtaatcgc gattatcatg actattttag
     6961 ttttagaact tcatgaacct aacggagaca cgtgggaggc gctcgctgca ttaggttacc
     7021 gattttttat ctatattgtc agttttatca ttttagcgat ttattggaac aatcatcatc
     7081 atttatttca agttgtacat aagataaatg gacgggtctt atgggcgaac agtttctttg
     7141 tttttgcgct ttcgcttttt ccgtttgcaa cttcatggat cagtgagtac atcaatagcc
     7201 tggctccaga aatgacttat ggttttgtta ccttgaccgc taatattgcg tattatatct
     7261 tggcaaaaga acttgtcaaa gcaaataaag gccacgagaa agcattgaag gcgatgttga
     7321 ataattacaa gaaatcgtat ctatcgattg gtttgaatgt gttaggaatt gtcttagggt
     7381 atttagtagc gccagtagcc gtgctgatcg caaacatcgt gattctagct gcttggatca
     7441 tcccaagtcg taccatcgaa tcacaatatc ggtaaaatga tccgtgttta aaatttaaga
     7501 gaaaaatata gaaaaaataa ataaacgatc aaatatccga gtgatactag tattcaaaga
     7561 gggatgcaga tggatcaaaa aagaattcat gtaggactga ttactagttt tgtcacgatg
     7621 cttatttatt tggatcaagg tcatttgact tggacagtag ttggtgtcgt aggatttttg
     7681 acattattca cagcaataaa aaaataaaca tctttatgta atctgatgtt ctatctattt
     7741 aagaaaagca tgtttacagc agtatgattc tgttgtaaac gtgtttttat ttgtggaaaa
     7801 tttaattttt agaaaagtgt atcgaaaaat agatagttag tttttccaca gtggataact
     7861 tcgtaagaga taaaaataaa aaattagtca aagaagggcg tatagatttc ttttgatagg
     7921 agatatacaa cagaaatgaa taaatattaa cgtgttttca aacagtataa aacggtgtgg
     7981 tttttggaat aaaaaaaata acatatctca ttttacgctt ttgttatttt atacttaaaa
     8041 aataacgaca cagaaaatac cgtgtctcag atttctctac tacttctaaa aactactgtt
     8101 tttttccttt gaaatgcctt caaaaagtag gatcaggcag aatatactgg cagaatcaag
     8161 gagaagggag cctaaataag gagaaaaacc atagaaaata taaagtaatt acttataaaa
     8221 caagcttttt atagaaaaat aatatacctg agtagggtat aaattaagac ggaattcctt
     8281 tctagattga gatattgact aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa
     8341 aacccttttt gttaaataag gggaatatag cctgtcagtt tcaaactgca agtcgtcctg
     8401 ctgcaataat atagtagatt agagatgaaa atagattttt ttaaaccaat gactgaataa
     8461 aaagaccgtc atagatacac attggaatgt gtctattacg gtcttttttt acgaacgatt
     8521 ttattgaaga cggacagttc gggtcaaaaa taaaaaagct acgatcagaa ataagaataa
     8581 aatgtatccc aaaataatgg ctagacggtc taacttattt gtcgatagat gtttgtttag
     8641 aagttgatag gcccataaac ctatcagtcc tcctgttgta ttagttatca aatcagtgat
     8701 gtccgctacg ccaataccga ataggaattg aatcaattct gccaagaagc ttacaaggaa
     8761 aataatccct aattttttag aaaaagaaag tctcttgaaa ttgacattca aaagcacccc
     8821 aaaaggaaaa aagacaatca agttataagt ggtctcacgt aataccgtcg tattttcaaa
     8881 agaaaaagta gaaaaaggga cgagattgac attgctgtaa gaataagtga gaaattgtgg
     8941 aagattaaat gatagcttga ataaaatcaa ccataataga aagaataaat aaatccctaa
     9001 taaaaatttt gatacatacc gcatagacat ctcctccatt taaaaagtat accatcgaaa
     9061 accgctgaaa aatcataaaa aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa
     9121 attagaagct tttttttgtt gggaatccgg tttctttaac aaagctaaga tagcgagttg
     9181 cttggaaaag ttgctatctt ttttctgtca aaacgactcg gaatgttcgt tttttgttct
     9241 aatttctcca agttgtttac tttttgatac taagtttatg aaaaatcaag aaatagttgt
     9301 tatactaggt aatgctgaca aacgggattg gcatttcatc ttggttcagc tttttttcca
     9361 attactagga aggagggata gattgttcaa gacttatttt cgtttcaata aaatacaaga
     9421 taatcctttt cgtatcatga atctgatgct acttatcttt attgtattat ggttaggtta
     9481 cataaatcat gattttttga tttcaagttt ttcatcatta gggatcttta cttttttcta
     9541 ttatcaaaac ataccgatga aatatttgct gaaacgtatg tttctgatcg gttgcggact
     9601 gttggttgct ttcgttttgg ggatcttatc gacttatgtt ttatggcttg aaccatttgc
     9661 cgtagctttg gttgcttttt ccagcagatt cgtgttgcga ttattccata tttctaaacc
     9721 aggtggcttg ttttttgcga tgctttctgc catgggaacg agcatgcagc tgccaatcgc
     9781 tcaattaccg atcgtctcac tttatttttt tatgggtgtc gtttttgccc tgattgcggc
     9841 agtgattaca aaattactgg attcacggcc tgagcagaca attgaaaaag caactttaaa
     9901 agaaagattt cacgaagagc ctttagtgat cattgattct gttttctaca gcgctgcctt
     9961 atttctttcc gtttatgtca gtcacggctt gaatctgcat aatccttatt ggttgacctt
    10021 gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg atgaagcatc gacaagtcca
    10081 atatctgatt ggatcgatgg gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt
    10141 tacgcagttg cagaccattt ttctgatcac ttttctatat ggaatcgcac aatttctagt
    10201 tgctagaaat tatgcagttg ctaatatttt tcttaatccg atggcgttga tgctatctac
    10261 acttattcgt ggagcatact tgatttcttt gatcgaatat cgttttttag ggatcgtcat
    10321 aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg gttggtttgc aacattattt
    10381 aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat atgtgccaaa agaggttatg
    10441 acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa agccagattc tcagcattaa
    10501 gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa aattttatat ttattctatc
    10561 aaaaaatttt atatttattc tatcaaaaaa ttttgtttat gatggtaact gaataaactt
    10621 tcctgagatg gtcaatgctt tccttgaaag tcttacagat attctttctt catagaaagt
    10681 ttaagtttta tagttagtgt acaataaaag tatgtttata ccatatggtg tccggataat
    10741 cagtcattta aacaatgagg ttcaaagata tgaaaagggg aaatttgatg aagaaaataa
    10801 gcaatgaaat ccaaattctg gcagaaactt taactcttcc caaagtcgtt atagagcaaa
    10861 tgattgtaca agcaaaaaac atccttgatg aggtgtttga gcaatatagt catgaactaa
    10921 tggatcctaa taaagcaaag gcgactgcaa agaaaataga caaagtctat gaagaggaac
    10981 cttttgcagc attagctatt tatctatatg cagcttctca aagttggaaa aaaatttatc
    11041 aaccattgaa gattccaaga actatctatt tggcaacaat gaatgctttt actagattta
    11101 ttcatgaaca tttccaagta acaggaagct atcgctttga ccgtggtttt tggatttggc
    11161 attacactag tggcttgatc tttcggattg gtgaattgga gtacgaaatg acatattttc
    11221 cgaaagggta cacggatcta agattagaag gaaagcagtg cctttctctt catattcctt
    11281 ccgatgcaga tttatctcag aataagataa gagatagtta cgaaagtgcc attcgttttt
    11341 ttgaacttta ttttcctgat taccattacc aagtgatgta cacagatact tggttactat
    11401 cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat ctgtcttttt gcagccgatt
    11461 atcgattatt atcagttgat gaacaggacg atagtggagt cccttggatc tttggacgag
    11521 tagatgccca aatacatgat tatccagaaa gtacgagttt acagcgacaa gcaaaagagc
    11581 aattgcttgc aggcgagcac attggctctg gcttaggtat tcttacttca taagtttagt
    11641 acttcgaaat agtttaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
    11701 taaacacgta taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca
    11761 aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt
    11821 cccagcctct tttttagtgt tctatcgaac acacagtacg ttcaataggt aaactagcta
    11881 taacaataga tattaaagtt aacggacaag agggcctttt ttaggtagct aaaacgtaat
    11941 aatgaaaatt tatttccaga gataggagtc aatcagctag tctgatcgac tgttttaaca
    12001 agttgacttt cattttaact tttgatacca tttaaaacac aaatcgtaat tgtttcgatt
    12061 tgtgtttttt agcatgacta caatcaaaga agaaagtggg tcgtccatat ggaaaaaaca
    12121 gatatctcaa gtgcataccg ccgcttgaaa agcccaaata tcaaaacacg aaaacgtgcg
    12181 ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa ggtaagaaga aggaaactta
    12241 attaaaaaaa tagccgaaaa acgtttttcg gctattttca ataatcggtt tttgtgtaag
    12301 gtttccgaaa gaactgctgt tgattttcca tttttaacat atgataaatt ggtagaatct
    12361 ttcttttcaa attgatacca ttccctaatt ccagttatat agttaactac agaaccacaa
    12421 agcacgtacg tttataaaag tattatctgt agttattttt caattccctt tcaacctctt
    12481 attttgacca gtagtattga tcactttacc ttttaacacc tttgttcgtt tttgaaacat
    12541 ttttagtcaa gaatggaaac gtaactacat aatcaaaggt tttgcgaaat atttcatgaa
    12601 atatttcatg tagaagcgag caatacggtt gcaaataacc attatcgttt aataagcagt
    12661 tacgaaagct cccaatatct ctaacgttca gctcactcca tttcttttct tcaaaaaaat
    12721 cagtgattcg tatctctttc tatttgtcga aaaagttaac tttaaaaagt aacgccgaaa
    12781 aaatatgcaa aaaaataaaa aagcagttac tattgctaga ataacatcaa ggaataataa
    12841 gttttggttt tcagataata gtcgataagt tgtgtattgg aattttagat acagtaataa
    12901 agtatttttc atgaaatttt gtgagaatca ctattacaag tatctttaat aaaattaatg
    12961 gttaaggagg aacttaaagt agaatgtaaa tgaatagttg tagtcagtcg attgtgatac
    13021 tagactcaaa gttgcaggaa taagtttttc aatttgtgat tggaagataa aaaatgaaaa
    13081 agaagggctt ttttttatac tttgttgatt gtagtgttta ttttccctct gtttagccaa
    13141 gtaacatttt ctatgactca accagtttca agtgaggaac atgataacct ttttcgtgct
    13201 acagaagagt ttaagaaata tcaggaaaat attgttccgg agactccatt agctagtgag
    13261 ccaattactg atattgatgg aaaccaaata ggatggtata tacagtacga agttatggtg
    13321 gtaaataatg actcaatagt aaaaaataca gtaggcttag agctaacacc gattgaaaag
    13381 gtgagttcat tgttaacgta tatttatact gatgaaagtg acgagactga gatcatttta
    13441 acggattact caaaaattgc tactgataaa aagtatatat tgtggatgta gacactagtg
    13501 aaatacttta tgatatagaa attgatgaaa atttactaga tgtagcacaa gaaatcgatc
    13561 atcaaaagca ggaagctatt cgatatgctg gtagaaaagg gaaaagaaaa tatttattac
    13621 gtgaatgtag caaaagttag agaagatgaa aatgagtgga aagaattcaa gagtcgctac
    13681 tcaattaact cgactccgac atttacagtt tacagagaag gatcgataga aaaaactgtt
    13741 ttttggacta aagaaagtgg aatgtcttta gctgaagtag aagagttttt ggattatgtt
    13801 agtatgcaac agtaagggag ttcttattat gaaaaatttt gacacatatt atgttattgt
    13861 tttttcgata tctttgctgt taatattact aactttaatg cagtataaag agtatccaga
    13921 gtattttatt ttaacttttg tatatttagt aagtagctac acacttctat ataaattcag
    13981 aagttcttct agagtttcta aagtttgttt cttaattagc ttattgattc tataatattt
    14041 ttgatttata taggaaatta aaaatattat agaatcaaca ctcagagaac gggttggttt
    14101 ttaatagatt taataagtac tgataatgaa atttctcctg gttgggaaaa cgaagttggt
    14161 taccctgtag aagaattttt tcaagattac tttgcggagt atgccgatag gataatgaga
    14221 gattacgtaa cagataaaac tcaagaagtc atcaatcaat cccagtcatt aaatgaatta
    14281 tctgataagg cgaaagaaga agcagttgcc attatttctt cagggattag tgactatatt
    14341 gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa ttgtacgcat gataaaagct
    14401 tttatcaaaa cttttgatgt gaaggcagat cttaagttta atgatactta cctagtagga
    14461 aaattaatta atatccttgg gacagagaaa gatacggaaa cgtttatttc ctctttgtct
    14521 atgttgttta cagataagca tttttcaact cagcttggtg gagaggaact agtggaaatc
    14581 ttttaaccat tacgcccagc attaataaaa gtatatcaaa aaagtactcc tgatgatttt
    14641 taagattggt ttgagaaata atgtgaaaac actattttta atgttaatta tagatagaga
    14701 tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga tacactgcta tatttcacta
    14761 aggatatagc agtgtatctt tcatgtcgtt tcactggcag ttttttataa aaggaggtgt
    14821 gcatttcagt tgcttttctt tggtagttag ttgaatcgtt taagaattaa cggatgtggc
    14881 agacaggtgc tctgtccaaa aaccagtaaa atctttgatg gttccatctt tatttagctg
    14941 tgcctgttca aatactgttt tgtttttacc gaatggatca attgcaataa ttgttccgat
    15001 ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct tcgataacaa gacaatcagc
    15061 cggagctaag ctcagtttgt ctgccgcttt aagaaaaata tccggttgcg gtttcccagg
    15121 gaaagagccg tcgtcataga caatcttgtc ccagtcaaac cagcgggcta aatcgaaaat
    15181 atcaaaataa aaagcaacat tctctttgac agtagccgtg gcaatcgtta atggaatctg
    15241 ttggtcagtc agctggtcaa gagtcgcttc aagccctttt gtcaactgaa gctcgttttc
    15301 attttgcaag caaagcatac gataatagct ttctttttct tctgacaata cggcgatctc
    15361 agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc tccgtattcg tccgaccatg
    15421 aatatttttt aaaatatcct cgtctgataa accatctttt gtatgatcat gtatcatatg
    15481 cagccaagca gcttcatgca gatgagagtc caaaaacatt gttccgttga aatcaaatat
    15541 aatccctttc atagttacgc tcctatagtg gtattttcgg ttgaatcagc aatttgcaga
    15601 tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga tctggcgatc cgtaatgagc
    15661 agatcgaaat catgcaaatt ataaaacaca taaaaatcat ctcgattgaa ttttttatta
    15721 tcaacgagta gatatttctt acgggaatta tttagagcga tctgctgtgc ttctccttct
    15781 tcggtactgt aagtagagat cgcttcgttg tgaacgccat ttgaactgat aaatgctttt
    15841 gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc cgacaaatgc gccggtattt
    15901 ttccggaaat caccgccaat cagtagaatt tctggtccat ctgcttgagc aagaatatca
    15961 aaaactggat aattatttgt aatgatccga attttcttgt tcagtaaatg cgtcgccaat
    16021 agttcgattg ttgtacctgg tcctaagaaa atcgtttctc cctcgtgaat caaggaggct
    16081 gctgtggcag caatttcttt tttttcttga atctgcagag tggctttttc agtgtgagaa
    16141 agttcttgat ctaaagtgta ggaaagactt tgagcccctc catggacacg cagcagcttg
    16201 cccgattttt ctaattcatc taaatctctg cgaacggtca tatcagaaac atctaattga
    16261 ttgatgattt cattaacggt aaggattcct tttttattga ccatttctac gatagtaagc
    16321 aatctttctt ttttcaacat tgtcttcacc tctaatttaa ggataatcta tcatataaac
    16381 ggacaacagt caatctaatt cgaacaaaag taaacattta gggtggtttt tatgtttgtt
    16441 acaatagatt ttattgctgt tttctggttt ttatgattat ttgtgtttgt ttttgttgac
    16501 aaatgttttg gatgcgttta caatgcaatc agttagaaag agcgaaaact gattatagat
    16561 ttgaggagag atgtacatgc ttaaattacc agaagatttt atttttggcg gagccacagc
    16621 agcctatcag gttgaaggag ccactaagga aggtggcaaa ggcgctgttg cttgggatga
    16681 ctttttagag gaacaagggc gattcagtcc cgatccggca agtgattttt atcatcaata
    16741 tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat ggtctaagat tatccattgc
    16801 ttggagccgc atttttcctg atggagctgg gaaaccaaat ccagaaggaa tcgcttttta
    16861 tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg ccgtttgtga cgttgcatca
    16921 ttttgatacc cctaaacgtt tgtttgacca tggagatttt ttgaatcgag aaacaataga
    16981 agcatttgtt tcctatgcca ttttttgttt tcatgaattc aaagaagtca aggtgtggag
    17041 tacctttaat gaaatctatc cagttgcaac caatcaatat ttgttaggtg ttttcccacc
    17101 aggaatcaaa tatgatttta caaagatcgt cgcctgtctt cataatatga tggttgctca
    17161 tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg ggtgaaattg gtgtagttca
    17221 ctcattagag acgaaatatg cggcaacaga cgcgccggaa gataagcatg ctgccttttt
    17281 agacgatgca ttgtcgatcc gtttcttatt agatgctacc tatttaggct attattcaac
    17341 tgaaaccttg actgctcttg atgagatatg tgaagcaaat caggccagct atcattttcc
    17401 tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt aatgactact tagggatcaa
    17461 tcactaccaa tgccattttg tcaaagctta cgatggagaa aatgcaatcc atcataacgg
    17521 tacgggtgaa aaagggacct ctgtttataa agtgaaggga atcggagaac gtatttataa
    17581 agaaggaatt cctcgaactg attgggactg gctgatttat ccagaaggtc tatatgactt
    17641 gttgctgcga attaaaagcg attacccaca ttacaacaaa atctatatca ctgagaacgg
    17701 gatggggtat aaagatcagt ttgaagatgg cattattatg gatcagccga gaatcgacta
    17761 tttgagagtc tatttggaat ccttaagcaa ggcgataaca gccggggtaa atgtgaaggg
    17821 gtattttttg tggtcgctta tggatctttt ctcatggaca aatgggtata acaaaaggta
    17881 tggcttgttt tatgttgatt ttgaaaccca gaagcgttac ccaaaagaat cagcttactg
    17941 gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa gtgataatgg aggaagaaaa
    18001 gatgaataaa gaggaattac aaatgcttgg ttttgagatc gttgcatatt caggcgacgc
    18061 tcgcagtaca ttattgaaat tattgaaaga agtaagacaa ggaaactttg aacatgttga
    18121 ttcggcgttg aaagaagccg acgaaaattt aacccttgca cataattcgc aaacaaaaat
    18181 tttagcggaa gaagcatctg ggaaagagat ggagatggga ttcattttta tccatgggca
    18241 ggatcattta atgaccactt tattgctgag agatctgatt caagacttta ttgtactgta
    18301 cagacagaat caaggatagg agaagaacgc tatggatgga atcattaaac aaatcgaaaa
    18361 agggaaacca ttttttgaaa aaatctcacg taatagttat ttaggagccg ttcgtgacgg
    18421 atttttaaca gcgatgccag ctattttatt ttcaagcatt ttcattttga ttgctgcggt
    18481 tcctgagatt tttggttacc aatggcctga atctgtctcc acatggctat ggaaagtcta
    18541 taattattcg atgggtgttg tagggttact cgtttcagca accactgctc gctgcttggc
    18601 tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc aacaccacct ctgtgatgct
    18661 ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg attgaaggtg gattttcaac
    18721 ggaatatatg gggacaaagg gactactggc ttcctttgtc gctgcatttt taacagttaa
    18781 tttatataag ttttgtgtgc tgaaagatat taccattaaa atgcctaaag aagttcctgg
    18841 aacgatttcg caaaccttcc gagatatttt tcctttctct ttttcagtat ttgcggccgt
    18901 tttaattgat ttaggcattc gacaaatctt ccaaatctca tttgcagaag caatcattac
    18961 ggtgctgcag ccgctattta cagcagctga cgggtactta ggaatcgcta ttatctgggg
    19021 agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg tcgatcgtgg agccggcaat
    19081 tgcggcaatt atctatgcca atgtggaaac caatctgcaa ttgttcaatt ccggtgaaca
    19141 agcttctaaa gtactgacgg tgggtttagg gaattttgta ggaactatgg gaggaactgg
    19201 ggcaacattg gttgttcctt ttatcttcct actctttgct aagtctaagc agttgaaggc
    19261 agttgggaaa gcttccttta ttccagtaag tttcgctgtc aacgaaccat tgctatttgc
    19321 ggcacccatg attttgaacc cttatttttt tattccattt ttgttaacac ctgttgtaaa
    19381 cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg aacagcttta tgtacgtttt
    19441 gccttgggca actccagcac cgatcggttt ggttctagga acgggcatgg gtatcttagc
    19501 ttttgttcta gcagcggttc taatcgtcgt tgacttcctg atctatctac cattttgcaa
    19561 agcatacgat gctgtcttag tagagaaaga agcacaaaaa gcagaggtga tcgcaaccga
    19621 ggaaggtgct tctgccgatg tacgtgagga agtacctgtg gcagcgagtc aagttatcag
    19681 cgatgcaaat aaagaaatca atgtgttggt actttgtgct ggcgcaggga caagtgcgat
    19741 gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa gcaccgatca gtgcaatggc
    19801 gggtgcttat ggttctcatt acgaaatgct gcaggatttt gatatggtgg tcttagcacc
    19861 gcaagtcaat tcttactatg atgatatcaa agaagacacc gatcgcttag gaatcaagct
    19921 ggttgcgaca aaaggagcag aatatatcgg attaacgaga gatcctgaaa aagctgttca
    19981 gtttattctg aaccaatttc aataaaggag gagccataat gaaaatggga atttggttgc
    20041 gtaaagctgt tttatttgca gctgtgttag gggctttggg ggctgtttca aaaactgttg
    20101 aagcatctgc cagcgaattt attcgcggag cagatatttc gatcctgtca gatatggaaa
    20161 aatctggtgc aaaatattat gaagacgata tttcaaaaga tgccttagag attttaaaaa
    20221 ataatggtgt caactatgtt cgactgcgtt tgtggcagga tccttataat agtaatggag
    20281 aaagttatgg agcagggaca aatgatctgg aaacgacgat tgaccttgca aaacgagcaa
    20341 aaaatctggg acttaaggtg ctgctggatt ttcactacag tgatttttgg gttgatcctg
    20401 ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt tgaagaaatg aatacggcat
    20461 tgtacgacta tacaaagaac gttctgtcgg aaatgaagca gctggacgtt tatcctgata
    20521 tggttcagat cggcaatgaa ctgaatagcg gaatgctctg gccttatggc aagtcttggg
    20581 gagaaggcgg gggagaattt gatcgattgg ctgctttttt gaagtctggt atccaagctg
    20641 ttcgagacac tcaacctaaa acaactccag taatgctcca cttagctgat ggtggcgata
    20701 caggagcatt tacttggtgg tttgatgaaa tcacgagtcg aggtgtgtca tttgatttaa
    20761 ttggagtgtc gtattatccg tattggcacg gttcaatgaa tgaacttcaa accaatatga
    20821 atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt tgaaacagct tatgggcata
    20881 caacagcgaa tgccgatact atgccaaatg cttttggtga agccgaagca gctgcggggg
    20941 gatatgaacc aacaccgaca ggacaagcag agtatttgct ggatcttgcc gatcggattc
    21001 aggcagtacc gaataatcgc ggggcgggct tcttctattg ggaacctctt tggtacaacg
    21061 gaaatgtgag ttgggcaaca caagcaggta tgaattatct cagtgttcaa tccactatgg
    21121 gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg aaacgccttg ccaagcttgc
    21181 gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa aaatgcaagt ttcgaaagcg
    21241 atggaccaac gaccagtcct tccaattggc aaacctggtt tcaacaagga accgctaagg
    21301 gtacgatcaa aacggaagcg ggcacttttt ttggggacta caaactaact ttttggcagg
    21361 atcaagcata cgaggcatca gcttatcaaa atgttagtgg cataagcaaa ggaacttaca
    21421 cattgagtgc ctatgtaatg tctgggggag agtttgatac taatcagtta tatatcaaaa
    21481 attatggtgg agcggaacta aatactgcgg ttgttaaaac aagtgaatgg acaaaaatta
    21541 caatcaaaga cattccaatt acaacaggtg tttgtgaaat tggggtttat agcaaggctt
    21601 caggaaatgc ttggacgaac gttgattttg tttcattggt gaaacaatag actgcggatc
    21661 gtttcgattc aagatcggag gttcccttca gccacatcgt tagcgtagaa ggcaggaatc
    21721 gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga tttccagtca tttttaccaa
    21781 aacgagtaaa gcaccagact tcatctacac tttgtattat gctaaagaga gttcaacgat
    21841 ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag ataacataaa gtttgtttaa
    21901 gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga gcacttagat ggatagaaaa
    21961 aagttctcgg aaaacagttc gtttttcgag aacttctttt tatgataaga atgaaacaaa
    22021 ttaacttata ttatagtctc ttttggtagg gaaccatctt atttttaatg aaaatcaagc
    22081 attttagcta ttgaagaatg ttgatttata gggattcccc ctccatatta tgttggctct
    22141 ttattcccac tctgttttca acgtaaagtg tataaaacag aatgcgatac tatcaaggga
    22201 gttaaaacaa tatacagaac agtaaaacac tataaataaa aataaaagtg caataaaagt
    22261 gcaatttttg cacctaatca tactctgttt atatatgaaa actagattct gaatccaata
    22321 tttggttaac gagaggcgtt cgaatttgaa cgtcttttat atattacttt caaataaata
    22381 tgaggtagaa tactatctat tttcttttta acccaattgt tataatggag agtgtaaaat
    22441 attttgtgta aatagaaaaa aggaagtccc ttctgtagaa tagagttacc acaacacatt
    22501 cacagaagag aggacttccc tatgaacgat tttactacag aaattctaaa gactctagcg
    22561 aacaaaggcg atttgaatga attattccgt gtccatttgg agaaagcagt caatacgctt
    22621 ctcaaaacgg agttaacggc tttcctagat tacgaaaagt atgatcgcat tggttttaac
    22681 acgggtaatt ctcgtaacgg ctcctatgac cgtacggtca agaccgagta tggggaactt
    22741 catctccaga ttccgcgcga ccgcaatggt gagttcaagc aacagactgt tcctgcttat
    22801 agacggacga atgacacgtt agaggagacc gtcattcacc tcttccgaaa aggtattacc
    22861 atgtcggaaa tcgcagactt gattgagaaa atgtatgggc attactactc gccccaaacc
    22921 atgtccaata taacaaaatc atttacagaa gaggtaacgg cgtttaatgg gcgggagctt
    22981 catgaccgtt atgctgctat ttatatggac gcaacgtata ttccgttaaa gcggaaaacc
    23041 gtcgccaagg aagctattca tatcgcagtt ggcattcgcc cggacggatc aaaggaagta
    23101 ttgagctatg cgattgcacc gactgaatcc atcacgattt gggaggaaat tttattggac
    23161 cttcaagagc gcggtttgaa aaatgtcctc ctgttcatca cggatggctt aaaggggatg
    23221 gtaggagcga ttagtcggtt ctatcccaaa gctcgttttc aacattgttg tgtacacgtt
    23281 tcccgtaata tcagtcataa agtgcgtgtc gatgatcgta aggaagtctg tgatgatttt
    23341 aaaatggtgt atcaagcctc atctaaagag gtggcgttgg aagcacgtgg tacttttgcg
    23401 gaaaaatgga aaaccagcta tccaaaagtg gttgaatcga ttctttcgaa cgatcacttg
    23461 ctcactttct atgatttccc cttggccata cgcaagagta tttattctac gaacttgatt
    23521 gaatccttta ataaacaaat c
//
LOCUS       DACIRY010000038        22864 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.038, whole genome shotgun sequence.
ACCESSION   DACIRY010000038 DACIRY010000000
VERSION     DACIRY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.038"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(153..551)
                     /locus_tag="IYE22_001257"
     CDS             complement(153..551)
                     /locus_tag="IYE22_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ9506477.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IYE22_001258"
     CDS             complement(548..1321)
                     /locus_tag="IYE22_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ9506478.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IYE22_001259"
     CDS             complement(1318..2001)
                     /locus_tag="IYE22_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ9506479.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IYE22_001260"
     CDS             complement(2168..3055)
                     /locus_tag="IYE22_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9506480.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IYE22_001261"
     CDS             complement(3052..3921)
                     /locus_tag="IYE22_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9506481.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="IYE22_001262"
     CDS             complement(4058..5344)
                     /locus_tag="IYE22_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9506482.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="IYE22_001263"
     CDS             5617..6195
                     /locus_tag="IYE22_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ9506483.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="IYE22_001264"
     CDS             6195..7124
                     /locus_tag="IYE22_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9506484.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="IYE22_001265"
     CDS             7121..8857
                     /locus_tag="IYE22_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ9506485.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IYE22_001266"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IYE22_001266"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ9506486.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="IYE22_001267"
     CDS             complement(9753..10262)
                     /locus_tag="IYE22_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ9506487.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IYE22_001268"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IYE22_001268"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ9506488.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="IYE22_001269"
     CDS             complement(11563..12654)
                     /locus_tag="IYE22_001269"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ9506489.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IYE22_001270"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IYE22_001270"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ9506490.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IYE22_001271"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IYE22_001271"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ9506491.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IYE22_001272"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IYE22_001272"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ9506492.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="IYE22_001273"
     CDS             complement(16617..17591)
                     /locus_tag="IYE22_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ9506493.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="IYE22_001274"
     CDS             complement(17572..19455)
                     /locus_tag="IYE22_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ9506494.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="IYE22_001275"
     CDS             complement(19473..21698)
                     /locus_tag="IYE22_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ9506495.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="IYE22_001276"
     CDS             21813..22847
                     /locus_tag="IYE22_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9506496.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT         6564 a         4979 c         4121 g         7200 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atgcaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaa
//
LOCUS       DACIRY010000039        22550 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.039, whole genome shotgun sequence.
ACCESSION   DACIRY010000039 DACIRY010000000
VERSION     DACIRY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.039"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            194..1168
                     /locus_tag="IYE22_001277"
     CDS             194..1168
                     /locus_tag="IYE22_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ9506497.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            1493..3388
                     /gene="asnB"
                     /locus_tag="IYE22_001278"
     CDS             1493..3388
                     /gene="asnB"
                     /locus_tag="IYE22_001278"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ9506498.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(3583..4029)
                     /locus_tag="IYE22_001279"
     CDS             complement(3583..4029)
                     /locus_tag="IYE22_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ9506499.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            4257..4406
                     /gene="rpmG"
                     /locus_tag="IYE22_001280"
     CDS             4257..4406
                     /gene="rpmG"
                     /locus_tag="IYE22_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ9506500.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            4494..5012
                     /locus_tag="IYE22_001281"
     CDS             4494..5012
                     /locus_tag="IYE22_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ9506501.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(5089..6045)
                     /locus_tag="IYE22_001282"
     CDS             complement(5089..6045)
                     /locus_tag="IYE22_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9506502.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            6211..7017
                     /locus_tag="IYE22_001283"
     CDS             6211..7017
                     /locus_tag="IYE22_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506503.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            7014..8003
                     /locus_tag="IYE22_001284"
     CDS             7014..8003
                     /locus_tag="IYE22_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ9506504.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            8000..9004
                     /locus_tag="IYE22_001285"
     CDS             8000..9004
                     /locus_tag="IYE22_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ9506505.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            9136..9678
                     /locus_tag="IYE22_001286"
     CDS             9136..9678
                     /locus_tag="IYE22_001286"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ9506506.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            9698..10396
                     /locus_tag="IYE22_001287"
     CDS             9698..10396
                     /locus_tag="IYE22_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ9506507.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            10417..10629
                     /locus_tag="IYE22_001288"
     CDS             10417..10629
                     /locus_tag="IYE22_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ9506508.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            10629..11591
                     /locus_tag="IYE22_001289"
     CDS             10629..11591
                     /locus_tag="IYE22_001289"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ9506509.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            11609..12004
                     /locus_tag="IYE22_001290"
     CDS             11609..12004
                     /locus_tag="IYE22_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ9506510.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            12167..12532
                     /locus_tag="IYE22_001291"
     CDS             12167..12532
                     /locus_tag="IYE22_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ9506511.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            12529..14340
                     /locus_tag="IYE22_001292"
     CDS             12529..14340
                     /locus_tag="IYE22_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ9506512.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(14402..14569)
                     /locus_tag="IYE22_001293"
     CDS             complement(14402..14569)
                     /locus_tag="IYE22_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ9506513.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            14818..15807
                     /gene="galE"
                     /locus_tag="IYE22_001294"
     CDS             14818..15807
                     /gene="galE"
                     /locus_tag="IYE22_001294"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ9506514.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            15819..17303
                     /gene="galT"
                     /locus_tag="IYE22_001295"
     CDS             15819..17303
                     /gene="galT"
                     /locus_tag="IYE22_001295"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ9506515.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            17327..18307
                     /locus_tag="IYE22_001296"
     CDS             17327..18307
                     /locus_tag="IYE22_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ9506516.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            18396..19220
                     /gene="tsaB"
                     /locus_tag="IYE22_001297"
     CDS             18396..19220
                     /gene="tsaB"
                     /locus_tag="IYE22_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ9506517.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            19204..19746
                     /gene="rimI"
                     /locus_tag="IYE22_001298"
     CDS             19204..19746
                     /gene="rimI"
                     /locus_tag="IYE22_001298"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ9506518.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            19753..20217
                     /gene="rimI"
                     /locus_tag="IYE22_001299"
     CDS             19753..20217
                     /gene="rimI"
                     /locus_tag="IYE22_001299"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ9506519.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            20214..21230
                     /gene="tsaD"
                     /locus_tag="IYE22_001300"
     CDS             20214..21230
                     /gene="tsaD"
                     /locus_tag="IYE22_001300"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ9506520.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(21802..22503)
                     /locus_tag="IYE22_001301"
     CDS             complement(21802..22503)
                     /locus_tag="IYE22_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ9506521.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         7110 a         3870 c         4766 g         6804 t
ORIGIN      
        1 aaggggctgt gacaagactt ttgtcacagc cccttattgt tgtactttta acctacaata
       61 ttttttgatt ccccctgagt actatttttt ataaatgcta gtaaatcaat cttctttggc
      121 cttttttttg ataaaaaaaa atactagatg taagataaaa gtgtaaaaac gatttgtttt
      181 aggagggaaa acgatgtgta ccgcaatcac ttatgcaacg aaagaccatt attttggaag
      241 gaattttgat tatgagatgt cttataatga agtagtcact attactccaa gaaattatcg
      301 tttcgatttt agaaaggtaa aaaatttaga taaacattat gcaatgatag gaatagcagc
      361 tggggtatcc aactatccac tctattatga ggctacaaat gaaaaagggt tgagtatggc
      421 aggactgaat tttcctggca atgctgatta taaagaatta caagaaggaa aagataacgt
      481 tgcaccattt gagtttattc catggatact agggcaatgc tcaaccatag acgaagctaa
      541 agaattattg gctactatca atctagtcaa tatcgatttt agtgaaaaac tacccttgtc
      601 acctttacac tggttattag ctgataaaga aaaatcgata gtaattgaaa gtatgaaaga
      661 tggacttcat ttatatgata atcctgtcgg tgtgttgaca aataatccgc catttgatta
      721 tcaattattt aatctgaaca attatcgctc gctatcaaat ggaacaccag aaaatcattt
      781 ttcaaaccag atcagcttga atgtttatag tcgtgggatg ggaggattgg gcttacctgg
      841 agatctctct tctgtttctc gatttgtaaa agccacattt actaaaatga atgctgcatc
      901 aggtgattca gaatctgaaa gtatcagtca attttttcat atactaggct cagtcgagca
      961 acaaaaagga gtatgcgata ctggagaagg aaaatacgaa tatacgattt attcttcttg
     1021 ctgtaacgtt gataaaggga tttattatta tcgtacgtat gaagacagtc aaattactgc
     1081 tgtaaatata aataaagaag acttggatcg tcatgaattg gtcagttatc cagtcataaa
     1141 agagcaacag ataaactatg tcaattaatt aatgaataga tccgtttggt atcatatttt
     1201 ctgatacagt cataattaga cacgatcttt aggtcgtgtc tttttttgct gttttttttg
     1261 gaaatagcat ccactaatat ttttttaaca cgaagagaat aaggtagcct caaaaaagaa
     1321 aacatggtaa attagatatt gggactttat tttctgtaaa aacagaaact ttcatctttt
     1381 gttttcacgt gacgaaattg taagagagaa aatattcgtc cgctatttat tctatgataa
     1441 gatgcatatg aatgaaataa acagtaattt tatattagga tgtgaaagtt aaatgtgcgg
     1501 tattgtaggt tttgttaacg acaaggacaa caaaaaaaca attattaata caatgatgga
     1561 tcgaatcgtt caccgtggac ctaatagttc aggagaatat atcgataaac atgttgcttt
     1621 aggattcaga agattaagta ttattgactt agagggcggt actcagccta tttacaatga
     1681 agatcggaca aagatcatta tttttaatgg cgaaatctat aattaccagc ctttgagaga
     1741 agaattgatt gcagctggcc acgtttttca aacacatgca gatacagaag tccttttgca
     1801 tggttacgaa gaatggggaa cagaattatt gcaaaagatc cgcggaatgt ttgcttttgc
     1861 tatttgggat aatgaaaaga atgagttatt cggtgcaaga gaccactttg ggatcaaacc
     1921 atattattat gcagaaatga acggaacatt tatgtttggt tcagaaatca aaagttttct
     1981 gcctcatcct gattttaaca aagaattgaa caaagaagcg ttgaaacctt atatgacttt
     2041 ccaatattct ccattaaacg gcgaaacatt cttcaaagat gtctatcgtt tgcctgaagg
     2101 ccattattat acgtataaag atggaaaact agacattcag caatattggg atgcagattt
     2161 tgaaacaaaa gaaacgcatt cccgacaaga atggatcgaa aaaatcgacg aaaccgttca
     2221 agcttctatt gaagcacata cggtcagtga tgtagaagtt ggttctttct tatccagcgg
     2281 ggttgattcc agttatgtaa cctctgtatt gaaaccagat cattctttct caatcgggtt
     2341 tgatgataaa acctataatg aagcgattga agccagaaaa ctaaccgaac tattggactt
     2401 agataataca gcagcagtga tcgacggaga catgtcgttc aaagcattcc ctttgatcca
     2461 gtatcactta gatgaacctg attcaaatcc ttcttgcgta cctttgtact ttttagcaaa
     2521 tcttgcttca caaagtgtgc gggtagtcca atctggagaa ggggcggatg aactgtttgc
     2581 gggataccaa acgtatggat tccatacgaa ttcaaaattc atccgtgtga ttgctcaagg
     2641 attgaaaaaa ttaccaaaag gaacacgcta taatttagga cgtaaaatcg gaaaaatgaa
     2701 aaatttccat ggacggatcc atttatatga atcacttgct cctgcaaaag aatattttat
     2761 cggtcatgct cgagtgttcg aagaatcaga agcatcagaa gtgttaacac caaaatatca
     2821 aacagcacca tctgtggatg aaatcatgac tgttcattac gagaaaacag aaggaatcaa
     2881 agatgaagtc aataagatgc aatatgtcga tcttcatcaa tggatgccaa aagatatctt
     2941 gttaaaggca gataaacttt ctatggcaag ttctttggaa gtccgagtac ctttattgga
     3001 tatcgaagtc atgaaattgg ctcaacaaat accaagtaag tttttattga atcaaaacaa
     3061 cacaaaagat attttcagac aagctgcgaa caaacatctt ccggaagaat ggtcgaatcg
     3121 tgtgaaatta ggattccctg tgccaatcaa agcttggttg aaagaagaac acggctacga
     3181 acaagtcaaa gcattattcg aagcagattt tgcaaaagaa ttttttgatc aagaaaaaat
     3241 catgaagttg ctagatgacc atcacgaagg acgtaaagaa gagcaaagaa aaatctggac
     3301 aatcttcagt ttcttgacat ggtataaagt ctactttgta gatgaaacga ttccgcaagc
     3361 agaagcaatc gactatgtga ctgtttgatg aaaatcaaat atcaataaaa aagaggtttt
     3421 ctcctaccgt ttaacactga gtgataagaa acaatttcaa aaaacagctc ttcccgtttt
     3481 atagatatta tgacttatgg ttcggagttg gcagaggaac accattgttt cgaacataag
     3541 ggggttgtga cacgattttc gtcacaaccc ctttacatat gttcaataat cgatttcttt
     3601 ttcttttgtc ccatcttttt tataaatgaa caaagttgat tctttgtttt gtgctacttg
     3661 ctttcctttt tcgattgctt ctttttttgt atcaaaatgg ttgcttgcct tttttgcccc
     3721 tttagatttc acgatccatt gttcttcctc atattctacg atcacatctg aatcaagcag
     3781 ttttccagca cgaggattcg tatcgtgttc atccgtcttg cttggatttt tttctttttc
     3841 gaaagttcgt ttttctgatt cactagcatc ttcataccat ttttttgctt gatcaatggc
     3901 aattgggatc gcacggttat cagggtaccc ttcatctaat aatgcatttg cgatgtcgat
     3961 tgcttttttt cttgtcaatt tatctaagtt tttcattgat gcaggaaagt ccttcatgtt
     4021 ccatggcata ttagtatccc cctttattaa aaaacataat ctatataaac aagtgtaagg
     4081 gtatttatac aaaaagaaaa agattttgtt tgagaagtaa aaccgttgta tttatcgtca
     4141 agtagtttta cttaacaaaa tcgtgaaaaa cactagcaat ctagaaaaaa tcatggtaag
     4201 ataatcaagt ctgagagtca tcttagagga ttcttgtaaa aatgggaggg aaaaacatgc
     4261 gcgtaaacat cactttagag tgtacttctt gtaaagaacg taactatcta acaagcaaaa
     4321 acaaacgtaa caatcctgat cgtttggaaa aacaaaaata ttgcccacgc gaacgcaaag
     4381 ttactttgca ccgtgaaaca aaataatgtt tttaaagttc tcaattcctt gctggtcaaa
     4441 gaattgagac tttttttatc tcaaaacatc taaaatcata aggagcggat aatatgttta
     4501 tttccatgtg ggcacaagat aaaaatggtc tgatcggcaa aaatggtttg ttaccatggc
     4561 gattgccaaa tgatatgcgt ttctttcgcg aacatacaat ggatcgaatt ttagtgatgg
     4621 gaagaaagac ctatgaagga atgggggatc tttcacttcc ttatcgccat atcatcgtgt
     4681 tgacaacgca accaaatttt cgcacgaaag aaaatgcgga agtaatgcat tcgatcgatg
     4741 aacttttgtc gtatagtcaa acgatctcag aagatatcta tgtttcgggt ggtagtcgga
     4801 tttttcaaga acttcttcct tacactggtc ggatctggcg aacattgatc gatagcacgt
     4861 ttgaaggcga tacgtatatt gggaatattg atttttcaga cttcgccctt atagaggagt
     4921 atgaggggat aacggatcaa gaaaatctat atgcccatcg ttttcaaaaa tgggaaagag
     4981 taaaaaatag ggttgcttca aaagaagaat aaaatctatg cgtctagttt gttaataaga
     5041 aaaaaagcat ctcacgacta atcgtgaaga ttcttttttt agttaaaatc aatttagttt
     5101 ttctttgata tcattgacca tttcctgact ggcaataggt ccgttatata gccaactaga
     5161 ttcaggtccg aattgcagta catgattgtc tgtcactgct ttaagattct tccagcttgg
     5221 ttcattgaac atggcagcat ctttgtcact gttcacaagg atgatgtagt cagcatctaa
     5281 ttcagctagc ttttcagagg aaacagcaga ccagtcagaa gtggcttttt tggagacctc
     5341 ttccactaga tcaggaacac caaattccag atcgtcgtat acgattcttc cacttgaccg
     5401 atttttgctg accataaagg ctgagttgtt ggtcacccac aacactgcag ctgtcttgtt
     5461 ctcgatttta tctgctagtt cttctcgagt tttcttgatg gatttttgat aatcggaaat
     5521 gattttttct gcctcttttt cttttgccaa agctttgccg acatctttta gttgttcttc
     5581 ccatgtgaca tctgttccgt tttttacaac ataagttggc gcgattttgc tatattgttc
     5641 gtatttcccg ccttcgacag tagcggaaga agaaatcaac agcaaatcag gctcaaagct
     5701 taacactttc tcataaggaa ggtcataaga aatcgttggg acatccttta aatcatcttg
     5761 cagataatct tgaatggaac cactgccaac agtccactgg gcgataggtt tttcccctag
     5821 tgcaatcaga tagtcttcta agtaactgcc gatgatccgt ttgggcgcgt ttggaatctc
     5881 tactttatgt ccaagtgtat ctgtgactgt atgagtacta ttggatgaag tcgtcgaatt
     5941 ggcagttgta gctgtttctt tatctgaaga gggggagcaa gcgctcagta ctccagccag
     6001 cagcaatatg ctagataatg tgaatacttt tgtttttaat ttcatttctt tcctcctaac
     6061 ataattgaga atctttctca ttggttatta ttcctgatat ttcgaacgct gtcaaccgct
     6121 tcatttggct atagatatga aagtagcgct tgacgaaatg ttgaaacagc gtcataattg
     6181 agaatgattt tcagcgaaag gatgggtgaa atgacgagat taagagcagc tgacattgca
     6241 gccggctacg aaaacaaaaa aatcattgaa aacatgtctg ttacgattcc tgaagggaaa
     6301 atcactagtt tgattgggcc aaatggaagt ggaaaatcta ctttattgaa aagttttacc
     6361 cgtatactac ctgtgaaatc aggaaagatc attgtcaata actctgatat ctctacatac
     6421 agttcaaaag aacttgcaaa aaaaattaca ttattagcac aaactagtga acaccctaca
     6481 ggtatgacag tagaagaagt agtatcttac ggacgatacc cttatcaaaa actgttcagt
     6541 ggcatggaca cgcaagatca tgaagcaatc gagtgggcgt tggatgctac aggtctagct
     6601 ggatttcgta tgcgggatat cgcaacgctg tctggtggac aaacccagcg tgtctggatc
     6661 gctatggcgc ttgctcagga aacagatatc ttgatcttgg atgaaccgac aacattttta
     6721 gatccggcac accaattaga gatcctacag ttattgactg aaatcaatca aatgaaacaa
     6781 acaacgatct tgatgtcgat tcatgacatt aatcatgctt cacgtttttc agattattta
     6841 ttagcaatga aaaacggtaa gttgatcaca gaaggtacac ctgaagaagt gatcacaaaa
     6901 gagtgtatga aaaaaatatt tgagattgat gcaacgattg tcacgcttcc aagaacaaat
     6961 aaaccagttt ttctaaccta tgatttgttg ccaaaagagc aggaggatca attatgagga
     7021 ctatgaagaa aaatagactc ctgcttttgg tctgttttgt tcttttgatt attttttttg
     7081 ttgctctttc aaatggcgca gcgactgtgg gaaaagcaga tttttggcag gcgttgtttg
     7141 attttgatag atcaaaacaa agtcaccaaa tcatacgaaa tattcgtttg cctcgtgtgg
     7201 cgggagcatt tcttgtaggg agttgttttg cattgagcgg tgcattgatg caaggagtga
     7261 caagaaatcc tttagcagat tcaggcttac taggaatcaa cgcgggtgct tcgttggcaa
     7321 tggcccttag ttttgctttt tggcctaaag cttctgattt ttctgtgttt ctaatgtcat
     7381 tggcaggttc ttttgctgta actttgctcg tatttggttt tttgcagttt tcgaaaatgg
     7441 gcatgaatcc tttacagctg attttagctg gcgtagctat tagttctttt tttactgcta
     7501 tcagccaagc acttacccaa ttctttaatc tgcagcagga tcttgctttt tggtttgtag
     7561 gcggagctgc caatatcaca tggaaacaat tagcagtatt gtttccttta tatatacttg
     7621 ctgttttggt ttctttttca ttaggaaaag caatatcttt gattcatttg agtgaaacac
     7681 atgcattagc tcttggaagt catcctaaac gaacgcggtt gattgttttc tgtctagttg
     7741 cagtgttagc agccattgcc gtttcgttag tcggacctgt ttcatttatt gggctgttgg
     7801 ttccgccagt tgttcgcgga atggttggaa cggactatcg ttatgttcta cctgtctctt
     7861 ttttagttgg cggtggattg gttatgttgg cggattttac agcgaggatg atcaatcccc
     7921 cttttgaaac accttttgga ttagtcattg cattgattgg cgttccgttt ttactgatac
     7981 aagtaaggag ggaacaacgg tgaaaaaaat atattggacg ttctatttct tgctttttgt
     8041 acttgtcatc ctgtctatag gtagtttaat gattggtacg ccttttattt acctggatca
     8101 gctttttgcc acgttcatag ggaaagaatc atcttctttg gcgctgattg ttagagaatt
     8161 tcgagttcct cgattactga tcagtttatt agcaggagct tgtcttggcg tttctggtta
     8221 tatcttgcaa ggagtaacaa gaaatgaatt ggcagattct agtatattgg gcattaatgc
     8281 cggagcagga tttttagtga tgctttattt aggtttcttt tcgcactatt cgctgccgtt
     8341 tctattgcta gttgtagcct ttctaggtgg tgttttagct gcttgctgtg tctatcttgc
     8401 ggcctatgat cgaaatggct ttttgggtat gaatcgaatc ttattgtccg gtattgccgt
     8461 taatgccggc ctttctgcat taacattgct atgcacgatc caattatcta aggaaaatta
     8521 tggatttgtg aatgcatggt tagcgggatc aatctggggt gcgagctggc cttatgtttg
     8581 ggcattgctt ccatgggcgg ttatcttaat tccgctagga ggactttatg cacagaggat
     8641 tggattgcta tcatttggtc aagaacgcgc gcaaggatta ggccttaacg tggctaaaag
     8701 tcaaaaaatc cttttgttgt tagctgttgc tttggcaagc ggtagtgtag cggtgacagg
     8761 tagtttatct tttgtaggac tactagctcc tcatgctgcg aagctagtcg tacgtaaaga
     8821 aaataactgg acctttgttc tgagcggttt gttcggttcc atttttctgc ttagtgcaga
     8881 tatggttgct agagtgattc taccaagtgg ggaagtacct acaggaattt tgattgccct
     8941 ttttggtgcg ccatattttc tgtatgcact ttttatcaga caaaaacacg cgctgaacag
     9001 ttgaaattta ttaaaaaagg aaggccaatc atttctaggt cttcttttta ttctatttat
     9061 gcatttattt gaaagttcga ttgctatttt tctttttttc ctataccatt ttatgagaga
     9121 aggaagggtg agtttttgga ccaattgaaa gtaagattac gccggaaagg aatcgaaaag
     9181 ctgaaaaatc tggccaaaag tggcaagaaa aaggaaaaag aacagcgaat ctataaacag
     9241 ctattcgcca gcgagtactg gaagtcagca aaagtgatag ggacgacttt atcgaatgaa
     9301 tttgaactgg atacacagcc ggttatccaa cgagcagaaa aagaacaaaa gaaaatagtt
     9361 gtacctaaaa ctttgccgaa acgtcagatg gcgttttatg aaatggatga acagtccgtt
     9421 cttgtacgtt cgagttttgg tgttcttgag ccacaatcag agaagctcta tttgccggag
     9481 atgatcgatc tcctcattgt accaggtatt atctacaata gagccggtta tcgaatcggt
     9541 tttggcggtg gttattacga ccggtattta aataattttc ataaaaaaac atgtagcctt
     9601 gtttttcgtg aacaattgaa tgaacaatgg caaccggaga gctttgatca gaagatccaa
     9661 caattgttta ttgactaaaa tgaaatggga ggttttcatg aattatcaac agcaaataaa
     9721 attgcgtgca tggttaagac gaccgttatg gacctatgct tttttaggga tacagacagc
     9781 ggtcttcctc atgatggaat tatttccgcg acttgagatc ccttattatg ccggaatgta
     9841 tggtccttat cttgttcact tcaatgaatg gtggcgttta gtgacaccaa tcttcatcca
     9901 ttttggactg atgcatttcg taatgaactc actgatactg tattttatgg ggcagcaaat
     9961 cgaggcaatc tatggccatt ggcgtttttt cttgatttat atgttcagcg ggataatggg
    10021 aaatacagca agctttgctt ttaacgaagc gaatgttctg tctggtggtg cgagtacttc
    10081 gatattcggt ttgttcggtg cgttgtttat cttagggttt cattttaaac ataacccagc
    10141 gatccagcag ctagtcagac attttctttt atttattgtg ttgacatttg tttttggtct
    10201 tctggatacc tctgtagatg tttggggaca tgtcggtggg ctaattggcg gattagtgtt
    10261 agggaatgtt ttgggtctac cgaaacaaca gacaagctat tcgatacacc aaaggattct
    10321 ttctacacta gtttttgtat ttctttttgt aatatgtatt ttactgggtt taaaaaaata
    10381 tggtttactt gtataatatg aaggaagtgc ttaaagatgg agagcttata tgatgtacag
    10441 cagctgttca aaaaatttgg catctttatc tatgtgggtg cacgcatcta tgacattgaa
    10501 ttaatgacta tcgaattgcg taatctgtat gatagccgtt tgattgatca tgatacatac
    10561 atgacagcgt ggcgcatatt aaaaagagag caccgaattg aagaaagccg aaagaaagga
    10621 aatttgtaat ggacaagaag attattggga ttgatttagg tggaacaaca gcaaaatttg
    10681 caattttaac accagaagga gagattcaac aaaaatggag catcgatacc aatattttgg
    10741 atgatggtaa gcatatcgtt cctgaaatca ttgaatcaat caaccatcgt ttgaatttat
    10801 ataatatgaa agccgaagac tttatcggaa tcggcatggg taccccagga agcgtggaca
    10861 gcgaagctgg aacggttatt ggtgcgtata acttaaactg gacagaagtt caatttgtga
    10921 aaaaactgat cgaggcagga acaggaatca aattcgctat cgataatgat gcgaatgttg
    10981 ctgctttagg tgaacgatgg aaaggtgctg gagaaaacga tccagacgtt gtattcgtta
    11041 ctctaggaac aggtgttggc ggtggtatcg ttgctggcgg aaatttgatt cacggcgtga
    11101 gcggagcagg cggagagatc ggccatatca ctgtggatcc tgaagggttc gaatgtactt
    11161 gcggtaaacg cggatgttta gaaactgtgt ctagcgctac tggtgtggta cgtctaggtc
    11221 gttacttggc agaagaatac gcaggagatt caaaactgaa agcaatgtta gacaacggtg
    11281 aagaagtaac aagtaaagat atctttgaaa tggctcaaga agacgatccg tttgcattga
    11341 tggttgttga tcgcgtatgc ttctacttag gtttagcttg cgccaacttg gggaatacat
    11401 tgaatccgtc aagtattgtg ttaggtggag gagtatcggc agcaggagaa tttttacgta
    11461 gccgtgtaga gaaatatttt aaagaattta ctttcccgca agtaagagaa tcaacaaaaa
    11521 tcaaattagc agagcttggt aatgaagctg gtgtcattgg tgctgcctca ttagccttga
    11581 aatttgctta aaaaaacgga ggcattacat ggttttatgg gtaattaata tcattttgct
    11641 attgattgtg cttgcaatca tcttttggga agtctatcta cgagtaatgg ccaagcgttc
    11701 agcaacgact ttgacagaag aagaatttcg agaaggaatg agaaaagcac aagtaatcga
    11761 tgttcgcgaa aaagatgtgt ttgatgctgg gcatatttta ggtgcaagaa acatccctta
    11821 cactgttttg aaagattcat taggatctat tcgaaaagac cagcctgtct atatttacga
    11881 tcaaaagaaa agcttgagca tccgtacagc aaataaatta cgtaaagcag gttatcaaga
    11941 gatttattta cttaagggtg gatatgacgg atggtctgga aaaatcaaaa gtaaaaaagt
    12001 ataaaacaga tgattgtatg acagctttaa aaaccgaatc aatcattaga ttttgaccaa
    12061 gatcacatga acggcgcaat ggatagttgt gccgttcatt ttttttattt tgtgctatta
    12121 tctacatata taagctaagc gaaaaggcag aaagaaggaa acaactatga tcaaattaaa
    12181 acgtgtttac cttgatgctg aaaaagagga tggctataga atactcgtgg ataggttctg
    12241 gccacgagga atttcaaaag aaaaagcgca gttagatctg tggttgaaag aaattgcacc
    12301 tagtaatgaa gcacgcaaaa cttttgacca tgtaccagaa cgatttgctt catttgcaaa
    12361 gaaataccag caagaattga ttttcgaaga agaaaagaag caagctattt ttaaactcag
    12421 tcaacttatg aaagaacatc cagtcattac ttttgtttat ggtgcaaaaa atgaacatca
    12481 gaaccatgct gttttattga aaaaatttat cgaaatggag gcatgcaaat gaaaataggg
    12541 atcattggtg ccggacctag aggattaagt atagcagaaa ggattctcca taataatcag
    12601 gacagacatt ctcttgaact ttttcttttc gatcccaatg gtcctggagg tcgagtttgg
    12661 cggttgaatc agccaccgga gttattaatg aattctgttt ctcaacaagt gacgcttttt
    12721 acagatgaaa cattgtccag tggaggattg gtaacacagg gacccaactt gtttcaatgg
    12781 agtaaaggac ctgctacaga ttatatcgaa cagacggtaa ccaaaaataa gcaactaatt
    12841 atagaagaaa tcaatcgtct ggaaaaagat cagcaatcta ctcgatgttt atacggactc
    12901 tatcaacgct ggtttttctc aaagttgcaa gaagaatttc cagcttctat tcagtttgtt
    12961 tattctttag taaaaaaagt cagcaaaaga aaagatggtt atcttttaca gacagatcag
    13021 caaagctatt ctatcgatca gcttgtttta gcaagcggtc actgggaaaa tgatgagaca
    13081 gaagaagaaa gagtgtgggt agactacgca aagaaaaatg atttactgta tcagcttcct
    13141 tctaatcctg cagatgtcga tctttcgaag ataccgccta aagaaaatgt ctttttaaga
    13201 ggtctaggct taagtttttt tgattatgtc ggattgttta cagtatcaag agaagggcgc
    13261 ttcgagaacg aaaaaggaaa acttgtctat catccatcag gcaaggaacc gattgtatac
    13321 agtggatcaa gaagggggct cccttattac cctcgaggaa gaaaccaaaa gcaaggcgga
    13381 tcaatggcat ggccacggtt actaacaaaa gaaaacctgg agcaatggca tcagtcggga
    13441 tttttgactg gcgaaacatt ttttgactat ctgaaaaaag acgttgagtt gttttattac
    13501 aagaaaatca tcgaagaata tcatttgccg attccaaaga aaaattttga gcatgatttt
    13561 ctctccctct cttcagagga gtgtctcgga aagcatccag cactaatccc ttatagatgg
    13621 gattggtcgt atttagaaat tcctcttacc tatcaggatg agtcctttgc agaagctagc
    13681 cgagcattta ttgaagggca gataaaagaa gcggaaaaag gcaattgcac aggagcattt
    13741 gcttcaacat ttgatgcatt aaaagactgg cgtgatccaa tccggcaggc tattgaatgg
    13801 cacgtttttt ctgcaaagga atacaaagaa atactatggg gatggtttac tcatctaaat
    13861 agctttttaa cgatcggtcc gccagtcatt cggacgaaag aattagcagc actgatagat
    13921 gcgggtattt ttcatttgtt cgaaccgcca tttgatattc agcaaaagca caaccgctat
    13981 gttatttctc tacaagataa agaaatcagc agtcgctatt tgattgaagc tcgattgcca
    14041 caaacaagct tttctcgttc aagtaatcct gtcataaaaa atttgaagga aaacaaactt
    14101 gttcgccctt tttcattttc agataataca ggaagttatc attcagaagc tatagatgtt
    14161 gagcgaggta cgaatcgcgt gattgatgct tcaggggaaa tccagtctga cttttattgc
    14221 tatggaatac ctgttgaagg tgtggattgg ctaactgcag cagtagctag accttataca
    14281 gatccatgga atctgagaca ggcagataaa atagcacaac ttatattgac tgacaaataa
    14341 aaatacgcat gatgacttat ctgttctttc acaagtcttc atgcgtgttt ttaaataatt
    14401 attaacgaga aacgcgtttg cgcattgctt ttttacggtt ttcttcgatc attgcttctt
    14461 tttcgtcaat tggatcgatc actgtttttt tcacagaagc gaccaaacca gcaacggcag
    14521 cggctgttgc cagacttcct actaatattc cagatacaaa ttttttcatt gaatccacct
    14581 catttattat ttgttatatc tattatggaa caaaagagaa aaaaaggcta gttttacgtt
    14641 tgtttgtaaa cctttgcaaa aaatccttga aaatgtttac taaatgtttt attataaatt
    14701 taccatatga atttacgaaa ttttactttg tttgttttca ccataaaagt aaacttcgtt
    14761 tttttaaatt atatataatc aacaaacaca attttactaa tcaaaggagt gttttttatg
    14821 acaattttag tactaggcgg agccggttat atcggttcgc acgctgtaga ccagctagtt
    14881 caaaaggggt atcaagtagc tgtggtagat aatttgctca caggtcacaa acaagcagta
    14941 catccagatg ctcattttta tgaaggagac atccgggata aagaatttct acgttccgta
    15001 tttgaaaagg aaccgattga aggagtaatc cattttgctg caagctcttt agtaggagaa
    15061 tctgtcgaaa aaccattgat gtatttcaac aacaacgtat acggtatgca aatcttactt
    15121 gaagtcatgc acgagttcaa tgtgaataaa atcgtgttct cttctactgc agcaacttac
    15181 ggagaaccaa aagagtctcc aatcacagaa gatacaccag ctaatcctaa aaatccgtat
    15241 ggtgaaagta aattgatgat ggaaaaaatg atgaagtggt gtgaccaagc atatggcatg
    15301 cgctatgtcg ctttgcgcta cttcaatgta gccggagcaa aagcagatgc atctatcgga
    15361 gaagatcata caccagaaac gcatcttgtc ccaatcattt tacaagtagc tcttggtcaa
    15421 agaaaagcat tggctgttta tggagacgat tatgatacac cagatggaac atgtatcaga
    15481 gattatgttc aagtagaaga tttgattgct gcacatattt tagctttgga atacttgaaa
    15541 gaaggaaatg aaagcaactt cttcaattta ggcagcagca aaggatactc agtcaaggaa
    15601 atgctagaag cagctagaga agtgacagga aaagaaattc cagcagaaat agcaccaaga
    15661 agagctggag atcctagccg attagtcgct tcaagtgaaa aagcgagaga ggttctgggt
    15721 tggaaacctg aatacacaga tatcaaagca attatcaaaa cagcatggga ttggcatgtc
    15781 agccatccaa atggttatga agaatagggg aatgagatat gtcaatcagt caaacgattt
    15841 gtgattttgc cacacttgcg atcgaagctg gcggatggat ggaaatggac cgcttatatt
    15901 tacagaatcg tcttttaggg atggtaggag aagatgctct agaaagcatt gaagtaagac
    15961 cagttaagga agaatctgta gacttattgg atcagcttgt cgctaaggca agagaaaatg
    16021 gtgttctttc agaattgcca gcagaacaag agatttttga agcgcaatta atggattttc
    16081 tcacaccgcc accttcagtc gtcaatgcat tctttgcaca gcactattcg aaaagcccag
    16141 aggaagcaac tgagtatttc tttaatttaa gtaagaaaaa tgactacatc aaaacaagag
    16201 ccatcgcaaa aaacatcaaa tttccggcag agtcggagta tggtgaactg gaaatcacaa
    16261 tcaatttatc taagccagaa aaagatccaa aacaaatcgc agcagaacgc gaggcggtaa
    16321 aagtagacta tcctacctgc atgctttgca tggaaaacga aggatataga ggtcgcttga
    16381 actatccagc ccgcaccaat caccggatta ttcgaatgaa tctagacggt gaaagctggg
    16441 gattccaata ttctccttat gcttattaca atgagcattc gatcatttta tcagaacaac
    16501 accgcccgat gaaaatcaca aaggaaacat ttgctagact gcttcggatt actgaagtct
    16561 tgcctcatta ctttgttggt tcgaatgctg acttgccgat tgttggagga tcgattcttt
    16621 ctcatgatca ttatcaggca ggacgtcatg aatttcctat ggcaaaagca ccgatagaaa
    16681 aacttatcaa actagcagat tatccgcaag tcatcgcagg agtcgtgaaa tggccgatgt
    16741 ctgtgatccg tttgcaatca acagagaaaa aattgttgat tgaagcagct aactcagtat
    16801 tggaaaagtg gaaagcatat tcggatgaaa cagtatctat cagagctttt tcagcagatg
    16861 gtacaccaca ccatacgatt acgccaattg cacgaagaag ggacaattac tttgagttgg
    16921 accttgtcct tcgagacaac aatgtttcag aagaacatcc tgatgggatt tttcatccac
    16981 ataaagatgt ccaacatatc aaaaaggaga acattgggtt gatcgaagta atggggcttg
    17041 ctgttttacc acctcgtttg aaaccagaac tagtagaagt agagcatttc ttattaggag
    17101 aagaaaacca gatggctgaa tatcatcgtc cgtgggcaga acaattaaaa aaagaacatc
    17161 ctgatttgac gaaagatacg gtcaccgatg tcgtacaaaa atctgtaggt caggtttttg
    17221 cacgtgtact ggaagacgct ggagtattca agcgagatga aaaaggacag gcagcgtttg
    17281 aacgatttac tgcaatttta taaacgtaaa aaaggaggag agacaaatgg caacaattaa
    17341 agacattgcg cagttggcag gagtttctcc tgctactgtt tcaagagttc tcaactatga
    17401 tcctgatctt tccgttggct tagccacaaa gcaaaaaatt tttgaagcag cagaacgctt
    17461 aaattatacg aaacatcgta aaaatgcagc aaatactaag caaacgcttc gcctgatcca
    17521 gtggtacgac gatacggaag agcttgaaga tctttattat ttatctattc gtctaggaat
    17581 cgaaaaaaag gcagaagaag caaacgttac tttattgaaa gaaacgtggg attccattag
    17641 tgatcttccg acagatggaa cgatcgcact aggaaaattc gacgaagctc agttattaga
    17701 aatgaaagga tcccaaaatg ccttgctttt ggtagattct gacggcactt ctcaaggaat
    17761 cgactctcta gttgtcgact ttaagtcaag tgttcaaaaa gtcattgaac attttgtctc
    17821 ccataaagcg caacatatcg ctatgcttgc tggaacagaa tatacgaaga aaaaccatca
    17881 gcggctaaaa gatcccagaa ttatggcatt tcaacaagtt ttggcagaaa aaggctacga
    17941 cgaaggtcca attatcgaag cagatttttc tgtcgagtca ggatatcaag cagtgaagca
    18001 gtatttaaaa acgaatccgc atgttcctga tgcgcttttt gcagctaatg atgcattagc
    18061 gattggcgca ctgaaagcga ttcaagaaaa aggtttagtg gtaccagaag atatttctgt
    18121 gatgggtttc aatgatgtca gtgtagcgaa atatgtaacg ccctctcttt ctactgtaaa
    18181 agtttatacc gaatggatgg gagagctagc agtggagacg attcttcaat taagtaattc
    18241 caaagcaccg gtttcaagaa agattattgt ggaaacagag ttgattattc gagaatcaac
    18301 gaaataaaag atggaaaagc tgtggggaaa tgaagtgata ccgcagcttt tttgtgtttc
    18361 aggaaggggc aaaatttgcc cgccagcaga taatcatgta tcattgtact agttttgcct
    18421 ttggtatttt catcctcaga ataaaaaagt atgctacaat ggaaggcgag aatgtcatag
    18481 cagaaaggat caaatatatg attacattag gaatagatac tgcaaaccaa actttggcga
    18541 tcggtgtggt agaagacgaa caaattcttg gacaaataca aacaaatatc aaacgcaacc
    18601 acagtgtaac actgatgcct gcaatcgacc aattgtttgc agatcttcaa ataagtccaa
    18661 aagacattga ccgaatcgcg gtttctgatg gtcccggttc ttatacaggg cttcgtatag
    18721 gtgtaacgac agcaaaaacg attgcttata cattagataa agagctagta ggagtatcta
    18781 gtctgaaaac catcgcggct aattgcatcg gtgtggataa aattattgtg cccatgtttg
    18841 atgctcgaag aaaaaatgta tacgctgggg cataccgctg gaaagaaaat agattagaaa
    18901 ttgtacttga agatgtacat atttctgctt ctgatttgtt cacacaacta caaaagctga
    18961 atgaagctat ttatttcgtc gggagtgatt gtcacaaatt cttctatgaa ataaaggaaa
    19021 tattgcctag tgcgatgatt aatacagttc cagcatggga tattccaagt ggtgtaactg
    19081 tggctcaatt aggtgcttta gctgaaccag ttgcagagaa acaagctttt cttcctcgct
    19141 atttaaaacg ggtagaagca gaagaaaaat ggttggagac gcacacacct ggagacgaaa
    19201 gttatgttga aaaaatttag agcgtatgcc agaagctttt ttcaagataa aatgatttat
    19261 acagaaaaaa caatcgaaca aatcgatcgt cgttacgtac taagagaatt gactacagaa
    19321 gatatcaaag agctgctgga tgtcgaacga gaagtctata atggcgaatt gccatggaca
    19381 aaaagtgcct ttatatcaga attgaagtca ccgcttcctc atctatatct aggtatcttt
    19441 gataatggaa gaatgattgg ttttatcggt tcaagagcgt tcggtttaga ttgccatatc
    19501 acgaatattg ccgttcgacc ggcatatcag cgtaaacgaa tcggttcgct gcttattgac
    19561 gaaatcgaga actttgctat aatgaatcgc tgtgagacga tgtcattaga agtgcgtatg
    19621 agtaatcaag atgcacaaag actttatcgg aagttaggtt ttcaagcaac aacgatcaaa
    19681 aaaagatatt acactgaaac aaaagaagat gcgttggata tgataaaact tttagaaaat
    19741 gagtgagatt agttgatttg cagaaaaaaa gattttgatg aagcgtactt ggcagaatgc
    19801 gtatggatga ttagcgaaaa tgcttatcca catggttcac cttggacgaa aatgcagttt
    19861 ttggcagaca tccaacagcc tcactctgat tatctgcttt tagttgaaga taaccagatc
    19921 tgtggcttta ttggatactc gaaagtaata gatgaagtcg agatcacaaa tattgctgtt
    19981 gcagtttcag aacaaggaaa agggcatgca cggcatttgt tacaattact gattacggaa
    20041 caaataaaag attcgctgca tgttttttta gaagtacgtc tatctaatga agcagcaaga
    20101 aaactttatg aatcagaaaa gtttcgtata ttaggtaaac ggaaatcata ttatcgcaat
    20161 ccgacggaag atgcgatcat tatgagcaga aagttaaaaa cggagacgat gaaatgaatg
    20221 aagaattgat tctggcgatt gaatcaagtt gtgatgaaac gagtgtagcg gtcgttagaa
    20281 atggaacaga gatcttatct aacatcgttg cttctcaaat aaacagtcat aaacgttttg
    20341 gtggcgtagt tcctgaagta gctagccgcc atcacgtaga acaaatcacc ttatgcttgg
    20401 aagatgcact ggtagaagct ggtgtgtcgg cagaagattt atcggcagtc gctgtcactt
    20461 atggaccagg acttgtcggc tcgttgctga ttggtatttc tgcagctaag gcttttgctt
    20521 gggcacacca attgccgctt attcctgtaa accatatggc agggcatatc tatgcagccc
    20581 gtctggttaa accattccag tttccattga tggcacttct tgttagcgga ggacatacag
    20641 aattagtcta tatgcaagaa gatggatcat atgaaatcat tggggagaca agagatgacg
    20701 cagcaggaga agcttatgac aaagttggaa gagtattagg tctttcttac ccaagcggaa
    20761 aagaaatcga tcagctagct catcagggaa aagataatta ccattttcca cgagcgatga
    20821 tccatgaaga taattatgat ttcagtttta gcggattaaa aagtgcattt atcaatttgg
    20881 tgcataatgc acaacagcgt ggagaagatc tagacaaaaa tgatttagca gctagctttc
    20941 aagcaagtgt gatcgatgta ttgattacta aaacacttcg agcttgtcaa aattatccag
    21001 tcaagcaact gattgtagct ggaggtgtag cggccaatca agggttgcgt gaaggattgc
    21061 aggatgcttt ggcaactaaa ctccctgagg tagaactagt gattccccct cttcgacttt
    21121 gtggagataa tgctgcaatg attggagcag cagcacatgt ggaaatgcaa aaaagaaatt
    21181 ttgctagcta tcagttgaat gctgatccaa gcttggtttt atctgaataa agaggttgtg
    21241 aaaaatctgc cctgcatcaa gtaataagaa cagcccagga aaaacggctt ctcatgtttt
    21301 tgacctggct gttcttatta ccgcgtatgc aagcttttga acgccgttta atcaagagtt
    21361 cgtaagcctg tcgtttaact ctgattcaca aggaacaatt tcaaaaaaca gctcctcatg
    21421 tttatgaaca ttatgacttg tgaccgaaga gttgacaggt acacaccgtt taatcgaaat
    21481 aagggctgtg acacgctttt gtcaaagccc tattttttac agcttattca tcattatccg
    21541 ttcctctgat tgccctcact gattaatcgt ttattgtcga ggaatatcga ataatctagg
    21601 tcggatgagg acaagacgct tcctctagtt aatctatcag tattttttgc aaacagtgaa
    21661 tattacataa ctgaaacaca catagaaaat ctttgagtct tgcccagtga tctaaaactg
    21721 ccggatcttt cagaagtttt agggaggaaa ataagcagtg accctgataa atatcagggt
    21781 cactgcttat ttttgttcgc gttattctct catagctgga gtagtagctg tcttacagaa
    21841 agtaaaacgc tttttgtcat aatctaacag tccggaatca cgtagttgtt tgattacttg
    21901 tccagcagtt tcacgtgtcg tgccgctatt cactgctaat tcttttaagg tgatcggata
    21961 aggaatggtg atatgaccat cttttgttgg tacgcccaat tcttctttca aaatgaataa
    22021 attttgtttt acccggtcaa aggcgctaga ggtcaatccg atttggatac gcaattcatg
    22081 tatttcaact actttagaaa ttttttgtat catatagatc aactgttcgg gattttttcg
    22141 tgctaatttt tcatagtagg aaacaggaag gtagaatgtt tcaatatcag tcatcgcttg
    22201 agctgtataa gtgtaggtct cttctccaaa cattcctgac ataggaaaaa ggttatttgt
    22261 attcacatag tcatagtagt aataagttgc actttcatca taacgttcta aacgaacgag
    22321 tcctttggtc aagatataca accgttgtct gggatcacca tcgtcgaaaa ggatctgtcc
    22381 ttttttataa gaacgaagaa cagtatgttc cttgatctgt tggaactctt catccgagaa
    22441 atgttcaaaa tcaggttgat atctgagttt tgaaaagtga aaatcgattt tattaacacg
    22501 cattaaaaag tcctctcctt attgttcact atatttattg tcactttttt
//
LOCUS       DACIRY010000040        21100 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.040, whole genome shotgun sequence.
ACCESSION   DACIRY010000040 DACIRY010000000
VERSION     DACIRY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.040"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(55..432)
                     /locus_tag="IYE22_001302"
     CDS             complement(55..432)
                     /locus_tag="IYE22_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506522.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            688..1416
                     /locus_tag="IYE22_001303"
     CDS             688..1416
                     /locus_tag="IYE22_001303"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ9506523.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1416..1670
                     /gene="purS"
                     /locus_tag="IYE22_001304"
     CDS             1416..1670
                     /gene="purS"
                     /locus_tag="IYE22_001304"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ9506524.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1672..2343
                     /gene="purQ"
                     /locus_tag="IYE22_001305"
     CDS             1672..2343
                     /gene="purQ"
                     /locus_tag="IYE22_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ9506525.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2344..4566
                     /gene="purL"
                     /locus_tag="IYE22_001306"
     CDS             2344..4566
                     /gene="purL"
                     /locus_tag="IYE22_001306"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ9506526.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4551..5990
                     /locus_tag="IYE22_001307"
     CDS             4551..5990
                     /locus_tag="IYE22_001307"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ9506527.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6022..7065
                     /locus_tag="IYE22_001308"
     CDS             6022..7065
                     /locus_tag="IYE22_001308"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ9506528.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7062..7640
                     /gene="purN"
                     /locus_tag="IYE22_001309"
     CDS             7062..7640
                     /gene="purN"
                     /locus_tag="IYE22_001309"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ9506529.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7637..9172
                     /gene="purH"
                     /locus_tag="IYE22_001310"
     CDS             7637..9172
                     /gene="purH"
                     /locus_tag="IYE22_001310"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ9506530.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9188..10462
                     /gene="purD"
                     /locus_tag="IYE22_001311"
     CDS             9188..10462
                     /gene="purD"
                     /locus_tag="IYE22_001311"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ9506531.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10584..11498
                     /locus_tag="IYE22_001312"
     CDS             10584..11498
                     /locus_tag="IYE22_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ9506532.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11631..13196)
                     /locus_tag="IYE22_001313"
     CDS             complement(11631..13196)
                     /locus_tag="IYE22_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ9506533.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13189..14640)
                     /locus_tag="IYE22_001314"
     CDS             complement(13189..14640)
                     /locus_tag="IYE22_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ9506534.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14654..15670)
                     /locus_tag="IYE22_001315"
     CDS             complement(14654..15670)
                     /locus_tag="IYE22_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9506535.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15890..16978
                     /locus_tag="IYE22_001316"
     CDS             15890..16978
                     /locus_tag="IYE22_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ9506536.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17032..18558
                     /locus_tag="IYE22_001317"
     CDS             17032..18558
                     /locus_tag="IYE22_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506537.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18560..19765
                     /locus_tag="IYE22_001318"
     CDS             18560..19765
                     /locus_tag="IYE22_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9506538.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20022..20999
                     /gene="guaC"
                     /locus_tag="IYE22_001319"
     CDS             20022..20999
                     /gene="guaC"
                     /locus_tag="IYE22_001319"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ9506539.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6763 a         3574 c         4799 g         5964 t
ORIGIN      
        1 tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc
       61 gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc
      121 gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt
      181 gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata
      241 aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa
      301 tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt
      361 atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat
      421 tgcatcttcc atcggcaagt gcctcctcga acccattaac caaattttta gttaattcta
      481 atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg
      541 ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat
      601 aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt
      661 taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa
      721 gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa
      781 gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat
      841 caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt
      901 gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg
      961 gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa
     1021 gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt
     1081 atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat cagtgagctg
     1141 aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg
     1201 gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat
     1261 gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa
     1321 gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt
     1381 ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa
     1441 cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta
     1501 tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg
     1561 atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg
     1621 taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt
     1681 gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag
     1741 atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat
     1801 ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct
     1861 cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc
     1921 ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga
     1981 cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg
     2041 gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa
     2101 ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc
     2161 tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa
     2221 gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga
     2281 tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta
     2341 tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc
     2401 gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg
     2461 cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa
     2521 aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct
     2581 ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa
     2641 agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga
     2701 atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg
     2761 ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc
     2821 ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct
     2881 tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat
     2941 atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca
     3001 ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa
     3061 cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg
     3121 tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca
     3181 gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg
     3241 acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg
     3301 gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa
     3361 ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa
     3421 tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa
     3481 gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag
     3541 ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag
     3601 caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc
     3661 aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa
     3721 gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga
     3781 ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg
     3841 gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta
     3901 tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca
     3961 ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt
     4021 ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca
     4081 ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa
     4141 aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag
     4201 agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac
     4261 tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg
     4321 atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt
     4381 gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct
     4441 gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag
     4501 atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg
     4561 aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg
     4621 ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta
     4681 tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg
     4741 tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac
     4801 gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc
     4861 atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa
     4921 aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc
     4981 atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg
     5041 aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg
     5101 acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg
     5161 cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg
     5221 gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc
     5281 agctttctat ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag
     5341 ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag
     5401 taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg
     5461 cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa
     5521 catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg
     5581 tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta
     5641 cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga
     5701 ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta
     5761 aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt
     5821 ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc
     5881 cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact
     5941 atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca
     6001 agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa
     6061 gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt
     6121 atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa
     6181 gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa
     6241 gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta
     6301 gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg
     6361 ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg
     6421 ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta
     6481 gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac
     6541 ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt
     6601 gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg
     6661 aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt
     6721 ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta
     6781 gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg
     6841 ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg
     6901 tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa
     6961 gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc
     7021 gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg
     7081 cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg
     7141 aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa
     7201 ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt
     7261 atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg
     7321 gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata
     7381 tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag
     7441 ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac
     7501 caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag
     7561 aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa
     7621 atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt
     7681 agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa
     7741 gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc
     7801 tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg
     7861 aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga
     7921 ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat
     7981 agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa
     8041 aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga
     8101 attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt
     8161 tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga
     8221 aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga
     8281 aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc
     8341 agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc
     8401 tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa
     8461 tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc
     8521 agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac
     8581 agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga
     8641 agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca
     8701 ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga
     8761 tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac
     8821 tccagcagaa gcaaaagcat tagtgtttgc ttggagagct gtcaaacatg tgaaatctaa
     8881 tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg
     8941 agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt
     9001 agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat
     9061 aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa
     9121 taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt
     9181 cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa
     9241 acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac
     9301 aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt
     9361 tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg
     9421 gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc
     9481 tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac
     9541 agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg
     9601 tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac
     9661 cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga
     9721 tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc
     9781 gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt
     9841 tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat
     9901 ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat
     9961 gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga
    10021 aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct
    10081 ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt
    10141 agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta
    10201 ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt
    10261 ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta
    10321 tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt
    10381 aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa
    10441 tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata
    10501 atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg
    10561 tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag
    10621 cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga
    10681 ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg
    10741 gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc
    10801 tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac
    10861 atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt
    10921 tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg
    10981 aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa
    11041 tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct
    11101 tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta
    11161 tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat
    11221 ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg
    11281 aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg
    11341 cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg
    11401 tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt
    11461 acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa
    11521 agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct
    11581 gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt
    11641 ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta
    11701 aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata
    11761 cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa
    11821 tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat
    11881 gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata
    11941 aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga
    12001 gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg
    12061 attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct
    12121 ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga
    12181 gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat
    12241 aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct
    12301 tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt
    12361 atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca
    12421 atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct
    12481 acctgataac tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg
    12541 aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa
    12601 taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat
    12661 acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca
    12721 ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa
    12781 acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt
    12841 tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt
    12901 acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga
    12961 gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt
    13021 tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca
    13081 ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt
    13141 tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt
    13201 gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg
    13261 gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt
    13321 gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc
    13381 tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa
    13441 ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt gacggacgta
    13501 cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga
    13561 aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat
    13621 ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc
    13681 ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc
    13741 ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa
    13801 atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt
    13861 caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt
    13921 aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga
    13981 aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa
    14041 gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt
    14101 taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac
    14161 acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat
    14221 gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg
    14281 ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag
    14341 tgtatttgta ttatttttgc tctctcgaat atcattccta agttcttcta ggatattttc
    14401 ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac
    14461 tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg
    14521 ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa
    14581 tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat
    14641 gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga
    14701 cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat
    14761 aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg actacacaat
    14821 tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga
    14881 tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca
    14941 tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc
    15001 gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag
    15061 tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg
    15121 attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt
    15181 ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg
    15241 cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat
    15301 tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta
    15361 atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg
    15421 caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga
    15481 gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc
    15541 agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg
    15601 tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa
    15661 cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg
    15721 gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag
    15781 acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc
    15841 tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa
    15901 atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg
    15961 tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa
    16021 atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg
    16081 ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga
    16141 aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt
    16201 aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt
    16261 actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt
    16321 atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac
    16381 gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt
    16441 gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac
    16501 gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga
    16561 taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga
    16621 tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa
    16681 acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc
    16741 cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga
    16801 aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga
    16861 taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa
    16921 ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga
    16981 ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat
    17041 tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc
    17101 gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga
    17161 aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa
    17221 atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc
    17281 atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt
    17341 tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa
    17401 acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag
    17461 attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt
    17521 ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg
    17581 agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg
    17641 aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag
    17701 acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt gggcagagat
    17761 ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa
    17821 gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg
    17881 catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa
    17941 ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag
    18001 gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat
    18061 gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg
    18121 acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta
    18181 actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt
    18241 gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca
    18301 caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat
    18361 gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct
    18421 tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt
    18481 attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg
    18541 aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa
    18601 actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt
    18661 agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa
    18721 tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt
    18781 agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc
    18841 aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg
    18901 tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat
    18961 accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt
    19021 tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta
    19081 tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat
    19141 catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt
    19201 atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt
    19261 actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct
    19321 tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat
    19381 ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat
    19441 cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc
    19501 tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc
    19561 ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat
    19621 tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt
    19681 agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta
    19741 taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca
    19801 agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag
    19861 actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt
    19921 tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat
    19981 gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg
    20041 aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa
    20101 cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga
    20161 ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc
    20221 ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta
    20281 cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag
    20341 aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta
    20401 ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg
    20461 ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg
    20521 ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct
    20581 ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg
    20641 gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca
    20701 tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg
    20761 gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa
    20821 atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg
    20881 aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca
    20941 ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa
    21001 aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag
    21061 ccaaacaaaa atagttcttt atatgttttg tttggctgga
//
LOCUS       DACIRY010000041        20818 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.041, whole genome shotgun sequence.
ACCESSION   DACIRY010000041 DACIRY010000000
VERSION     DACIRY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.041"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(39..1240)
                     /locus_tag="IYE22_001320"
                     /pseudo
     CDS             complement(39..1240)
                     /locus_tag="IYE22_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1357..1872
                     /locus_tag="IYE22_001321"
     CDS             1357..1872
                     /locus_tag="IYE22_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9506540.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2071..2517)
                     /locus_tag="IYE22_001322"
     CDS             complement(2071..2517)
                     /locus_tag="IYE22_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ9506541.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IYE22_001323"
     CDS             complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IYE22_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ9506542.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4396..5025)
                     /locus_tag="IYE22_001324"
     CDS             complement(4396..5025)
                     /locus_tag="IYE22_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ9506543.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5009..5473)
                     /locus_tag="IYE22_001325"
     CDS             complement(5009..5473)
                     /locus_tag="IYE22_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9506544.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5960..7228)
                     /locus_tag="IYE22_001326"
     CDS             complement(5960..7228)
                     /locus_tag="IYE22_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ9506545.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7459..8196)
                     /locus_tag="IYE22_001327"
     CDS             complement(7459..8196)
                     /locus_tag="IYE22_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9506546.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8263..8832)
                     /locus_tag="IYE22_001328"
     CDS             complement(8263..8832)
                     /locus_tag="IYE22_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ9506547.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8960..9832
                     /locus_tag="IYE22_001329"
     CDS             8960..9832
                     /locus_tag="IYE22_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9506548.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10174..11046
                     /locus_tag="IYE22_001330"
     CDS             10174..11046
                     /locus_tag="IYE22_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9506549.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11043..13001
                     /locus_tag="IYE22_001331"
     CDS             11043..13001
                     /locus_tag="IYE22_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9506550.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13252..14103)
                     /locus_tag="IYE22_001332"
     CDS             complement(13252..14103)
                     /locus_tag="IYE22_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9506551.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14226..14693
                     /locus_tag="IYE22_001333"
     CDS             14226..14693
                     /locus_tag="IYE22_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ9506552.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14956..15438)
                     /locus_tag="IYE22_001334"
     CDS             complement(14956..15438)
                     /locus_tag="IYE22_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9506553.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15538..16401)
                     /locus_tag="IYE22_001335"
     CDS             complement(15538..16401)
                     /locus_tag="IYE22_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9506554.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            16999..17847
                     /locus_tag="IYE22_001336"
     CDS             16999..17847
                     /locus_tag="IYE22_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506555.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18018..18359)
                     /locus_tag="IYE22_001337"
     CDS             complement(18018..18359)
                     /locus_tag="IYE22_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ9506556.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18445..19152
                     /locus_tag="IYE22_001338"
     CDS             18445..19152
                     /locus_tag="IYE22_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ9506557.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19139..19462
                     /locus_tag="IYE22_001339"
     CDS             19139..19462
                     /locus_tag="IYE22_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ9506558.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19551..20588)
                     /locus_tag="IYE22_001340"
     CDS             complement(19551..20588)
                     /locus_tag="IYE22_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9506559.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6202 a         4309 c         3695 g         6612 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt ttcaaaccga tccgactaga taagaaacct gtaaatagca
     1021 tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag
     1081 cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag
     1141 cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg
     1201 tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct ctattagcat
     1261 catacatgat ttattttaca tgtaaatatg cacatgtaaa acgtggaaac tttactgtga
     1321 tataataaag aaaataagac gagaggtagg atttttatga atactttagc ctatatatta
     1381 ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt
     1441 ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt
     1501 tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat
     1561 gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa
     1621 gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga
     1681 ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg
     1741 aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga
     1801 attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag
     1861 ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc
     1921 ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc
     1981 tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc
     2041 caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac
     2101 acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat
     2161 tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac
     2221 accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat
     2281 catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac
     2341 accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa
     2401 caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac
     2461 catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct
     2521 gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa
     2581 gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa
     2641 agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta
     2701 aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac
     2761 ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac
     2821 ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa
     2881 gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca
     2941 tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt
     3001 ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa
     3061 tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac
     3121 ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt
     3181 ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt
     3241 ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt
     3301 gtttagagga agacaaccga tccggtctga tttgttgctt tttcttttta ctggacactt
     3361 tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct
     3421 ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga
     3481 gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt
     3541 tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat
     3601 aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct
     3661 cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt
     3721 cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg
     3781 ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga
     3841 aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga
     3901 gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga
     3961 cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta
     4021 tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat
     4081 cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta
     4141 ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca cctattctac
     4201 tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg
     4261 aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc
     4321 cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata
     4381 gtaactgaaa accttttatt cgaacgtagt gatcttttct ggtgctaaac gaacagactc
     4441 gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt
     4501 atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc
     4561 atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt
     4621 gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa
     4681 ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt tatagatttc
     4741 ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc
     4801 attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc
     4861 agcgaccaca aagcgccatg gttgcatatt gacagaagaa ggtgcagtcg tcgcttcttt
     4921 gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga
     4981 tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt
     5041 tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt
     5101 gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca
     5161 tttcacaatg ctcgattttc ttctgggctt tttctgttag cgctacatac acttcccggt
     5221 tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac
     5281 gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat
     5341 tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt
     5401 caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct
     5461 cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa
     5521 taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc
     5581 aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa
     5641 agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg
     5701 cagtagaaaa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg
     5761 aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg
     5821 tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac
     5881 caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca
     5941 gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca
     6001 tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt
     6061 catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt
     6121 cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat
     6181 ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc
     6241 aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca
     6301 atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag
     6361 ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac
     6421 cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt
     6481 tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct
     6541 tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg
     6601 cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc
     6661 cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt
     6721 tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg
     6781 cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag
     6841 cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt
     6901 gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct
     6961 ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt
     7021 cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac
     7081 tctctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt
     7141 ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa
     7201 cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc
     7261 taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt
     7321 gtttcttttt gataaatttg ctttttctac ataaaaagac gtaaatgaag aatattcttc
     7381 tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta attctctttg
     7441 gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca tgttactgac
     7501 agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcggaggt
     7561 caatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt
     7621 ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg
     7681 ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata
     7741 aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc
     7801 agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat
     7861 gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg
     7921 catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc
     7981 gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc
     8041 ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag
     8101 tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc
     8161 catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa
     8221 atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac
     8281 aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt
     8341 aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt
     8401 tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct
     8461 tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa
     8521 tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa
     8581 gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt
     8641 caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg
     8701 ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttcagctt ggatctgatg
     8761 gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat
     8821 gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg cttatcatgt
     8881 ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt
     8941 taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag
     9001 agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa
     9061 acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcggg gctcccgaga
     9121 gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt
     9181 ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat
     9241 tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa
     9301 ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa
     9361 actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata
     9421 ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc gaaatcatcc
     9481 tttagcaaag caaaccgtca tccatccgca ggaactgaaa actgttcctt tgatcatttc
     9541 ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt
     9601 atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac
     9661 ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa aaatattcat
     9721 cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct
     9781 ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt
     9841 tttttccttc gctttcagct ttttaacaac gaataagata agcaaaagat ccaacgatta
     9901 aataacataa gtttcttaat ttatttcttc cttttatttc tgataattcc agtaaattac
     9961 tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa
    10021 caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg
    10081 tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa
    10141 cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa
    10201 aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga
    10261 tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg
    10321 gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta
    10381 tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa
    10441 catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt
    10501 atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt
    10561 attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac
    10621 gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat
    10681 cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg
    10741 gaaacagata tccattcaga accattttca ctgaattatt caagactggt gaatcattta
    10801 cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta
    10861 gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg
    10921 ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat
    10981 atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa
    11041 aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac
    11101 ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg
    11161 tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg
    11221 ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt
    11281 tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga
    11341 atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta
    11401 ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga
    11461 tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt
    11521 tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt
    11581 ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc
    11641 gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag
    11701 caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa
    11761 tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta
    11821 tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ctagcagcag
    11881 cttttgcttc attctttact ggtgtgacag aaccacttga attttcattt atgttcgttg
    11941 cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat
    12001 tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt
    12061 tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag
    12121 tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc
    12181 gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg
    12241 cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg
    12301 ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata
    12361 aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca
    12421 tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg
    12481 cagcacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtgaaacga
    12541 tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag
    12601 atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag
    12661 aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg
    12721 gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact
    12781 taaaaggcga accattcact ttatatgtag aagaaggtca aaaagtcgca agaggtcaat
    12841 tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg
    12901 ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa
    12961 aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac
    13021 ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg
    13081 aataaaaacg gcgttctcct gccaactccg cggtaataag tccagctaaa cgaaacatat
    13141 gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct ttttcacaaa
    13201 atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg
    13261 atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc
    13321 taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa
    13381 catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc
    13441 acttgcttca ataatttcta actctttacc tgtcgcacgc tctaatgcct tggctaattc
    13501 ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac tttctactag
    13561 tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt ttccatttcc
    13621 tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta attcattctc
    13681 tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc
    13741 agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt
    13801 ataagcgata taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc
    13861 ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc
    13921 tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag
    13981 tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt
    14041 ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg
    14101 catatttgcc actccctatt ctttttattt gttaagattt acagctgtac atttataaat
    14161 tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac
    14221 aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa
    14281 tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg
    14341 cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa
    14401 ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct
    14461 atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt
    14521 gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa
    14581 aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt
    14641 tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc
    14701 tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg
    14761 acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca
    14821 tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca
    14881 aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc
    14941 acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt
    15001 cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc
    15061 tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg
    15121 cgattctgct aaagcgctta caacaagata cgttcctttt gggaatggga tgattcgcgt
    15181 agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt tcccgttata
    15241 gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt
    15301 tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc
    15361 cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc
    15421 gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa
    15481 attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta
    15541 tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca
    15601 ttttgtaaaa gagatctcgg gagcttcgac taacccataa tctgaagaag gaagccattc
    15661 tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa
    15721 aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt
    15781 tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc ggtctccatc
    15841 aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc
    15901 ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc
    15961 aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat
    16021 acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga
    16081 tttttgtatg ccgtatctac tgatttctga aggcagatat cctgaccaat cacgaaaagc
    16141 tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga
    16201 tccatctaga agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag
    16261 cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt
    16321 ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa
    16381 ttggttcaga tctttaagca tatttatctt cctccttgat tgccttttta tcatattatc
    16441 aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt
    16501 atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct
    16561 atctatgttt tacctttaga aaaataaatt ttggggaaga cagattctaa gtaaacaaag
    16621 cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa
    16681 ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc
    16741 tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata
    16801 aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt tttctcacat
    16861 tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt gattatcaaa
    16921 tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata aataagtgtt
    16981 tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa cgttttggaa
    17041 cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac aagcaagtac
    17101 ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg ggatgaaaag
    17161 accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa acacatggct
    17221 gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc actttccgaa
    17281 ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt aacagagatt
    17341 cttatagaac atccgcactt tatcgaacgc ttcaaagtta agatgccatt tcatgcaata
    17401 aattattcag ataaattaac aactatcatc cataaatacg atcatattat caagaaaaac
    17461 aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa agttcatcca agaagcagtt
    17521 gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa tgaagtgatt
    17581 gctactctcg aaacattcta ttgggagcct gaggatgaac gaacccttct tctgatagag
    17641 gactgttatt atttatggaa agaattatta gcagaagcac ctcatgcaga aaaaaggcaa
    17701 atgttctctt ggttcgtctg ccaagtcgat catactgacg cctcttattc aaaacgctac
    17761 tctataaaaa tcctgaaaga agactttcgg gaaaaagaat tttctaacca aaagaaaaaa
    17821 atagataaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa tagcaagtaa
    17881 acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta aaaacacgaa
    17941 tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc atttgactat
    18001 taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg atagattttc
    18061 cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg ttccgctgca
    18121 tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca ccctaaccct
    18181 agtgcagcaa tcattgttgc gcctaatgca ggtccttcct ccacacttaa acaatagacc
    18241 gttgcatcga aaatatcagc ttggatttgt agccatgcaa gatttttcgc gcctcctccc
    18301 accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt tacgtttcaa tcccaccatt
    18361 gacaaatgct cattaaaaat ttagaatatt cacaagttta acgacaatta tgaaaattta
    18421 tgtcgtgcta aaggagtttt gttattggat gaaaagttag atataaaaac tgcgatcaaa
    18481 gacacgttac ctaccgtttt cggttatatc ggtattggac ttgcatttgg tatcgttggg
    18541 aaagctgccg gatttcatcc actagtcgtc acgttgatgt ccctgctggt ctatgctggt
    18601 tctgcccaat ttatcacagt cagcatgctt gctagtcaca gcccattgct ttccatcgtt
    18661 ttctcgacct ttctagtcaa ttcccgaatg attctgatga gtatgacgat tgctccttat
    18721 ttcaagaaaa atcggctgct tcaaaatctg ctgattggca cattgctgac agatgaaagt
    18781 ttcgcactgg gaatgaataa attgaactat actggacaaa agttaaactt tcgttggatg
    18841 aatacagcta attggatttc ttatttgacg tgggtgggtt cttctcttgt tggcgcactt
    18901 ttagggaatt ttattactga tccgaaaaag tttggtttgg atttcgccat cgtcgctatg
    18961 tttatcgggt tactttatct tcaagtcatt tctgatagaa acacctctaa acgattacag
    19021 ttgatcctga ttggtctcac tttgattctg gtatatgtcg gactgatttt tatcccaagc
    19081 aacttagtga ttgtagttgt aacactgatt gggtgcggat tgggggtgtg gatcaaacat
    19141 gccttctttt gagtttacat tgctgactat tttaggttgt actttagcaa cttggctttc
    19201 tagggtccta ccatttgtct tactgaaaaa attcgattta ccgcaaccgt tgcttgagta
    19261 tttaagtttt gtcccgatcg tcattatgtc tgctttatgg ttcagcagtc tatttaccca
    19321 aaatatcgga catctgccgc agatcaatct cgaaaatgct ttggcttcag ttcctacact
    19381 tttagctgct attctttcaa aaagcttatt ggtgatcgtc ttggcaggaa ttctttccct
    19441 ttcgttgatt cgcttattat aaataaaaaa acaagagctt atggcaaatt actgtcatgc
    19501 gctcttgttt ttatcaaagt caaataagaa aaaaatcggc gtattcttgc ttatccgttg
    19561 aacggaccac taacaacgac ttttcctaac atatgtcctg tttctacgtc tttggttgct
    19621 tgtttcaagt tcttcgcatt catcccatct gaatagacct tgctcaatgt actgcggata
    19681 atcccttcat ttgctaaagc agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca
    19741 tagttatagt ctgttttagc gaacatatac tcccagtcga agctgataga ttggtttttc
    19801 caaacaccta tatctaacgg ctctttgatc cctacgatcg ttcccacatg tccaaatggt
    19861 cggacaaggt gcttgatttg atcaaagtag cgggtaatat cgaataatac agcgacatac
    19921 tctacttgat cgattcctaa ttcacgaaaa cttttactta agtcgtgatg ataatcgatt
    19981 ggatagtcta cacctgtttt tttcagccat tcaaaattct tcggacttgc agtcgcgtag
    20041 acttctaggc cagcccaatg cgctagttga ttcaaaatcg aaccgacgcc cccggatccg
    20101 ttgatcacta agattttttt cccgcgattg gcattttctt ctggagttag accaaatttt
    20161 tcaaataaca attcataagc ggtcaatgag gtcaacggca aggctgcagc ttcttcatct
    20221 gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa ctagttggta ttcttgattg
    20281 cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc
    20341 acttcgtcac ctacagcaac aactttcccg actccatcaa aacctaagat acgtaaagca
    20401 tttcttatcc cgttatcttg gcgaagtttc gtatcgacag gattaacaga tactgcgttg
    20461 acttttacca gaagatcccg tgaactgggt acaggtatct gttctttttc atcaataaag
    20521 ctttttggat catcgatcgg caacccttca taaaaaccga ctaccttcat taattccttt
    20581 tctgacatac gaattcctcc ttagcttcat caagttggag catccataca gtgttcattt
    20641 caatatatat agtatgctat ttgttccacc ttttttattc atcataatta ttctcttact
    20701 taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga cttttgtcac
    20761 aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc acaagtcaaa atattcat
//
LOCUS       DACIRY010000042        20812 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.042, whole genome shotgun sequence.
ACCESSION   DACIRY010000042 DACIRY010000000
VERSION     DACIRY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.042"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1299
                     /locus_tag="IYE22_001341"
     CDS             <1..1299
                     /locus_tag="IYE22_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9506560.1"
                     /translation="QPKAEVLLIDEDHKDGLGYVSNGINLYLKGKIRSLAEVAHNMRT
                     LQESGAKLITEWRVTELDPDKHQLILASKEGKNETITYDKLIVATGSSPVTLYKQIEA
                     ENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLELADALRNKGYIIHLVDYMPNVL
                     TRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSEEKVVSVQLLSQAIKADMVIFSA
                     QTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYAIGDIVPVSFDKNKRHLFLPLVT
                     RAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFLGTVGLTEDEAPFLEQNTGSCSG
                     EFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQEFLLSDLNLLADIVKHKTTIPQ
                     LAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRVDKRL"
     gene            1470..2540
                     /locus_tag="IYE22_001342"
     CDS             1470..2540
                     /locus_tag="IYE22_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506561.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            2555..3223
                     /locus_tag="IYE22_001343"
     CDS             2555..3223
                     /locus_tag="IYE22_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506562.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            3243..3821
                     /locus_tag="IYE22_001344"
     CDS             3243..3821
                     /locus_tag="IYE22_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9506563.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            4224..5135
                     /locus_tag="IYE22_001345"
     CDS             4224..5135
                     /locus_tag="IYE22_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ9506564.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(5391..6308)
                     /locus_tag="IYE22_001346"
     CDS             complement(5391..6308)
                     /locus_tag="IYE22_001346"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ9506565.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            6510..6947
                     /gene="zapA"
                     /locus_tag="IYE22_001347"
     CDS             6510..6947
                     /gene="zapA"
                     /locus_tag="IYE22_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ9506566.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            7005..7553
                     /locus_tag="IYE22_001348"
     CDS             7005..7553
                     /locus_tag="IYE22_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ9506567.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            7668..10028
                     /locus_tag="IYE22_001349"
     CDS             7668..10028
                     /locus_tag="IYE22_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ9506568.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            10115..10429
                     /gene="trxA"
                     /locus_tag="IYE22_001350"
     CDS             10115..10429
                     /gene="trxA"
                     /locus_tag="IYE22_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ9506569.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            10611..12389
                     /gene="uvrC"
                     /locus_tag="IYE22_001351"
     CDS             10611..12389
                     /gene="uvrC"
                     /locus_tag="IYE22_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ9506570.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            12534..13433
                     /locus_tag="IYE22_001352"
     CDS             12534..13433
                     /locus_tag="IYE22_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506571.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            13426..14688
                     /locus_tag="IYE22_001353"
     CDS             13426..14688
                     /locus_tag="IYE22_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506572.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            14851..16749
                     /locus_tag="IYE22_001354"
     CDS             14851..16749
                     /locus_tag="IYE22_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ9506573.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(17048..17767)
                     /locus_tag="IYE22_001355"
     CDS             complement(17048..17767)
                     /locus_tag="IYE22_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ9506574.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            18104..18436
                     /locus_tag="IYE22_001356"
     CDS             18104..18436
                     /locus_tag="IYE22_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506575.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            18423..20426
                     /locus_tag="IYE22_001357"
     CDS             18423..20426
                     /locus_tag="IYE22_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ9506576.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            20445..>20812
                     /locus_tag="IYE22_001358"
     CDS             20445..>20812
                     /locus_tag="IYE22_001358"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ9506577.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILA"
BASE COUNT         7006 a         3507 c         4426 g         5873 t
ORIGIN      
        1 caaccgaaag cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt
       61 tcaaatggaa tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat
      121 aacatgcgga ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa
      181 cttgatccag acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata
      241 acctatgata aattaatcgt agcaacaggt tcctctcctg tcaccttgta taaacaaatt
      301 gaagctgaaa atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct
      361 ttaaaagaag caaaagaagt agtgatcttc ggtgctggtt atattggatt ggaattagca
      421 gatgctttga gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta
      481 acaagatatt tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa
      541 atcaattttt atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta
      601 tccgtacaac ttctatctca agcaataaag gcagatatgg tcattttttc tgcccaaact
      661 cgtcctaata cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata
      721 gtcaatgaat atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca
      781 gtcagttttg acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat
      841 atggcaagag cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa
      901 aagataacag cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat
      961 gaagcgccat tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca
     1021 caatacgatg aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta
     1081 gaaataattg gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt
     1141 gcggatatcg tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt
     1201 ctgtctgaac acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct
     1261 aagagagcaa atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt
     1321 gtgtggggat ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc
     1381 ggaagaattt tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat
     1441 aaaatgatta tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt
     1501 caaagttacg ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt
     1561 ttttgactgg gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg
     1621 aggcagattc gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta
     1681 ctaagaagat agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc cagacacctg
     1741 gagtcgtgac tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta
     1801 tcgataaaaa tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg
     1861 atgaggcagt aggcgatatt agtttgaaag aagaatatgg tctagccgta ggagatacag
     1921 ttaagttatc tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat
     1981 tcaatgtctc accagttctc tatacaacca tcaatgctta ccaagagatc cgcttcgaaa
     2041 aagaagatac aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta
     2101 atttacctga agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat
     2161 ataatgcgca agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta
     2221 ttgtgatcgg gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa
     2281 tgaaagcaca agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca
     2341 ttttatcatt tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc
     2401 tacctgatgc ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga
     2461 tgctggtagt agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc
     2521 cgttgaaggc aattggataa gaaggaggaa gagcatgaaa gcaatagaat ttaaagaagt
     2581 agaaaaaagt ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag
     2641 tatcgataaa ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt
     2701 tttgactttg gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca
     2761 agaattcagt aatcaaaaag aaagtgaacg cgttaaacta cgttttaaag aaattggctt
     2821 tattttgcaa gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt
     2881 agataagatc aaacatgaac atcgtcaaaa agaagcaaaa gaattatttg agcagcttgg
     2941 cgtggataag ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt
     3001 cgcaatcgct cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc
     3061 tagtttggat tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga
     3121 caaaaataaa gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag
     3181 aatatttatg atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa
     3241 aaatggtcca tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact
     3301 tgtttatgtc caagggatat ctcggcactt caacaagaga gatcgcacaa aaagttggga
     3361 tcacccaacc caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt
     3421 tagaaaacca cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa
     3481 cgggatttca agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg
     3541 atttgtttct gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc
     3601 ttttctttct atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc
     3661 aatctgtgct aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt
     3721 tagcacctta catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat
     3781 tgatcgattt atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa
     3841 aaatagttag gtgaagaagc aagatatgac gaagatcgtt atatcttgtt ttttttatat
     3901 aggtagtttt tcataaatac aaatttgtta ttctctcatg attatcaggg tatattttta
     3961 tttattatgt cggtgttcca ggaagtgttt taatttaata acgaagaaat taattgtttg
     4021 tagaatgaag gaaactattt attgggtaga tgaatcacca cgaatagtca aaaggagatt
     4081 tcaggatatg ccaaaaatga aaaaaatcat tttagcagta atgttatttt ctacggttat
     4141 atcttttaca gccccttttt tgcgtactta agctgatttt aagttttgac ccttagtctg
     4201 gatctacgag gtgagagtca agaatgaaaa aaataattgg agcttttgta tttgcaatca
     4261 ttatcattat tatctgtttt caaatagatg tttttggtca aaaaaagata gttggacaaa
     4321 cggtcagcag tacgaacagt acggttacaa agacaaagga atcatttgat gatacagaac
     4381 aagagacatt aatcaaaatc ggaaaaaatg accgtacact atatacagat gactcgctaa
     4441 ctaccccact tgcaactata aacggcggag agctggcgga gtttctttct gaaacaaaaa
     4501 atgcttatca gatcaagaca aatgatggct atactggata tttagccctc gaagatggaa
     4561 ccaaagtcac aaagaatata caaacaaaac cggaagagct atctgatgca gtgattgtat
     4621 tagatcctgg gcatggcgga aatgatactg gggcattaag caatgatgaa caaacagaag
     4681 aaaaagatat aacattaagt acagccataa aagtaaaaaa ggcgctagag gatgctggag
     4741 caacggttta tttgacccat actacagatg aattagtaca gcttggagat atatgcgact
     4801 acagtgaaga aaaaaaggca gatgtcttta tcagtttaca cgcagactct acggaatatg
     4861 cgaatgaagc aactggaatc acgacatatt attactatgg acaagaggaa acattagcac
     4921 agacaattgc tgactccttc actgatctgc ctctggattc aagaggaatc tccactggga
     4981 actatcaagt tcttcgagaa aatcttcaac cttctatttt aatcgagatg ggttatatga
     5041 ataatgatag tgatttaaag gaattagtaa cagacagtta tcagcagcag attgctgcaa
     5101 gtttgacaca agcattgacc acttattttc agtaataaat aagcccagcc aaacaaaaat
     5161 agctcctcat atttttcgtt tggccgggct tattatcgca gatgcaagct tttgaacacc
     5221 gtttaaggaa gaggttgtga gcctgccgtt taactccgcg tcacaaggag caatttcaaa
     5281 aaacagcttc tcatgtttga tgactattgt gacttgtgac cgaggagttg gcatgagaac
     5341 accgtttatt cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt
     5401 agcagcttca aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata
     5461 ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct
     5521 gatggaaggg tgattccagc ttcagctgat gctttgtgta actcttccaa aaagctggca
     5581 cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag
     5641 tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc
     5701 ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc
     5761 aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg
     5821 atctcattgt atttttctgg agataagaca agcagtttat aggggatggt tttttttaag
     5881 atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt
     5941 ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggacca
     6001 aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt
     6061 ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta
     6121 tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta
     6181 atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa
     6241 taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg
     6301 ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc
     6361 gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt
     6421 atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa
     6481 ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcag
     6541 tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg
     6601 taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata
     6661 atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag
     6721 aaagaatctt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta
     6781 cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat
     6841 tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt
     6901 taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca
     6961 aaataagaaa aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga
     7021 ttttattttt attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa
     7081 tcatattcgc gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat
     7141 tagcaagtca tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag
     7201 cattttttga tcaggtattt gcttttcacc tagatgaagc tttttatgca ggaactgctt
     7261 ttctgattat tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg
     7321 gattgacgta tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac
     7381 taatcatggc ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag
     7441 atgtggtcca aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc
     7501 catttcttac caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat
     7561 agtgctggaa aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg
     7621 tatgtccggc acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa
     7681 tccttgaaac attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg
     7741 cacaaggaaa agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa
     7801 actggcttca agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta
     7861 tacctaaact tgaaaatatc cgtccccata tgaaacggat cgagatcgga gcagacttga
     7921 atggagtgga gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt
     7981 tcattgatga tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc
     8041 agttagtcac gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac
     8101 gcgtgactga tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg
     8161 aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa
     8221 gtgatgcgat catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc
     8281 gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc aggacagact ttatttattg
     8341 aaccaaaaca agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa
     8401 atgagattca acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa
     8461 tcatccacaa tgcttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg
     8521 gtaaagaagt aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac
     8581 aagcaagaca tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa
     8641 aagactatca agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga
     8701 agactttagg tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag
     8761 aaagtcaaat cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg
     8821 agcaaagtct atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag
     8881 tcaatgaaaa aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag
     8941 gtgctgctct agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg
     9001 caacaacgca ttatcctgaa ttgaaagttt atggatacaa tcgggcaaat acgattaatg
     9061 ccagcatgga gttcgatgtg gatacactaa gtccaacgta tcgtttgctg attggcgttc
     9121 ctggacgaag caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg
     9181 atgaagcgaa gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt
     9241 tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg
     9301 aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg
     9361 aaaaagaaat ggaaaaagca aagaaaaaag caaacgaagt cgtgtcagag gcggaagaaa
     9421 aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg
     9481 tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa
     9541 ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag
     9601 acgaagtgtt ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc
     9661 agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg
     9721 ttgctcctca aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta
     9781 gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg
     9841 tagatcaata tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg
     9901 gaaaaggaac aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg
     9961 tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat
    10021 ttaagtaaag cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata
    10081 ctaatcatag attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac
    10141 atttaatgaa gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg
    10201 tccatgtcgt atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga
    10261 attcagaatt gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat
    10321 catgagcatt ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg
    10381 tgttcattca aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc
    10441 aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt
    10501 taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt
    10561 ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac
    10621 gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata
    10681 aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat
    10741 attttactgg cagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg
    10801 aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga
    10861 aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga
    10921 taacgaatga aaaatatccc cgtttggtga ttactcgtaa agtactaaaa gacaaagcac
    10981 tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc
    11041 gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc
    11101 atttgggaca atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa
    11161 tcgttgatga agtgaaaaag tttttaaacg gaggacacga aaaaatcgag tcagagatcc
    11221 atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag
    11281 accagatccg tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac
    11341 tggatcgaga tgtatttggt tatgcgattg acaaaggatg gatgtgtgtc caagtgtttt
    11401 tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag
    11461 cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc
    11521 ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta
    11581 cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgaataaga
    11641 atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga
    11701 tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt
    11761 cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg
    11821 atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg
    11881 atgattatgc atcaatgaga gaagtcatct atcgcagata ttcacgagtg attcgcgaag
    11941 ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa
    12001 aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg gcaaaaaacg
    12061 ataaacataa aacaaatgag ttgctattcg gtatagattt ggctgtagta ccgcttgagc
    12121 gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa
    12181 tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg
    12241 aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata
    12301 tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa
    12361 acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg
    12421 tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg
    12481 cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag
    12541 aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc
    12601 aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga
    12661 cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc
    12721 atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt
    12781 atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa
    12841 gaaaagagat tgaaccaaaa atcgattttt ggatggaaaa atttcaggta aaagggaaaa
    12901 agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca
    12961 cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg
    13021 taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca
    13081 ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc
    13141 gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta
    13201 cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga
    13261 aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg
    13321 ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac
    13381 cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg
    13441 attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata
    13501 ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat
    13561 agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc
    13621 caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa
    13681 gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata
    13741 aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa
    13801 caactgactg gcttgcaatc gatgcttcga gcaagtcaat taggcatttc accagaagaa
    13861 gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa
    13921 ggaaaaatga cagttggaga agataactcg acagtccaat atgtcgtaag ttatgtagca
    13981 acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct
    14041 tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat
    14101 ttttacggaa aactaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt
    14161 attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta
    14221 gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg
    14281 atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa
    14341 gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatatatg
    14401 cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat
    14461 attccattct tatcttcata tattatgccg gttcgcttag caaatgagac agtcggtatg
    14521 ggtggagcat tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg
    14581 tctgcaaaca tgtacaaatc gaatgtactc gtgtatagcg aaggcggatt atggagttca
    14641 ttgaaacaat cgatttcaat tatgagaaat gaacgaaaaa aagcataaaa tatagaaaaa
    14701 tatagaagga agctggacat ttattgttcc cagcttcttt tttgtataga cctatttcta
    14761 tttttttatt atattggata tatatattca ttgaaaatag agcataacaa ctttataatg
    14821 ttgagtatac caatttttgg aggcgtttta atggaccgct atttagattt actttcagag
    14881 aagttcccta caacggaaga tgtcatcacg gaaatcatca atctcgaagc aattttgcag
    14941 cttccaaaag gaacagagtt atttttgagc gatatacatg gcgaatttcc tgcttttgat
    15001 catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttctctgaa
    15061 caaatgacgg aaacggagat cagccaattc actattttta cggcttatcc agaatatgtg
    15121 ttgacgactg attggtacaa aaaacaagat aaatcccagc tgatccatcg tttgatcgat
    15181 cttttacggt ttacgacagt caaatacact cgatcaaaag ttcgaaaagg attgccgaaa
    15241 gaatatagct atatcattga ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa
    15301 gactatgtaa aaaaaatcat tgaacaactt gtcttgatga aggaagaaga aaaattttta
    15361 acaaaacttg cgcagacgat ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat
    15421 atttttgatc gaggaactca agcagccaaa gtaatggatc gaatcatgga tttttcctct
    15481 gtagatatcc agtgggggaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag
    15541 gcatgtttat tgacgctttt acggattgcc gcacgctaca actatttatt tgaattagag
    15601 aaagaatatg gattaaattt acgtccatta ttttcttttg cccatcaaac ctatcagaag
    15661 aatccagtat ttacccctaa aaacaggaaa gatctatctg aaagagagca agaagaatta
    15721 gaaaagatcc atcaagcact ttctatcctc caattcaaat cagaacatca gttgttgaat
    15781 cgacgacctg aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa
    15841 tcaacgatcg ttttagaagg tcagctctat gagttgaaag ggacttgttt tcaaacgatc
    15901 cagccagatg atcctaaaaa attcttggaa gaggaacaaa aagtgatcga tagtttgatg
    15961 tattcattcc agcactcgtt aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg
    16021 ggaatgtatc tcacgaacaa tcagcatgta ctgttccatg gctgtatacc tgtagatgaa
    16081 aaggggcagc tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctggga
    16141 ttttttgaaa aacagataac tgaaagcgca aaagatttga ctgcaaatga agatttggcg
    16201 accgatttga tttggtatgc gtggagcggg ccattttcgc ctttattcgg caagagtaaa
    16261 atggcaactt ttgagcgcta ttttctcaca gagcctcata cgcacgtcga aaaaagcaat
    16321 ccctattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc agaattcgat
    16381 gcaaaagaag aaggttcagc aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga
    16441 gagtcaccga tcaaagcaaa cggaaaagca tttgttattg acggaggtct atccaaagct
    16501 tatcaaaaaa caaccgggat tgctggctac tcattactat gcaattctta cggtttccaa
    16561 atcgtgacac attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa
    16621 acgaatgtaa aaaaagtgat cgagcagcag ctcccacgca aacttaatcg gaatacgaca
    16681 aaaggaatgg aattacagca acagattact caactccaaa ggctgttgga ttatcgaaag
    16741 gacgattgag aaaaatgcta gccaacattg ctcaatagac taaaaagcga ctgcaacaaa
    16801 aggcgtgtcg cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt agaagctatt
    16861 tttctgttta tttctctaat actcaggtca gggcgctgat ttcacgacct cttccttaaa
    16921 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccaacaaac atgagaagct
    16981 gttttttgaa attgctcctt gtgactcaga gttgaacggc aggctcacga cctaatcaga
    17041 aaaacgatta aaattcattc acaaagaaat cgaataaacg ctgttcagat gaatctacac
    17101 gatgagaaag ttccggatgc cattggacac caaggatttt gtgcatatta tctttacttt
    17161 ggactgcttc aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat
    17221 ctttgactgc ctgatgatga taggaattca caaagtatga atcagggaga aggtcactta
    17281 ataaactgtc cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt
    17341 gttcgtgttt tacggaccat tttggatata atgaaagatc ttgataaagt gttccctcca
    17401 acacgacatt caatagttga agtcctcggc agactgcaaa aatcggtttt ctttgtttca
    17461 atgcttccaa gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt
    17521 ttggatgagg gtcttccatg taaagttgcg gactaacatc ctgaccgcct gcaagaatga
    17581 gtttgtcgat tttctcaatt agttgaggcg ctgaatctgg agacatgact ggcagaataa
    17641 gaggtaatcc ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta tagcttactt
    17701 gatttccttg gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg
    17761 gtttcatttt tattccccct aaagtagact aattgtaaca aggatttaaa aaaatttaaa
    17821 ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa
    17881 ttttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag
    17941 taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga
    18001 aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta catactttca
    18061 atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga
    18121 agctttggaa caaatccgaa aagcagaaga agaaaatgag aagcgtgctc gaaccctacg
    18181 gcagcagttg aagacctatg cagaagaaaa agagcaagaa ttggagataa tccaaacgaa
    18241 cagtcggcaa cagctgaaac agttggtaga agaaaaagaa caattcgaaa agcaagaatt
    18301 agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga
    18361 ccaatatcaa aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac
    18421 ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga
    18481 ggattttaca agcagtacaa ggaatccaaa atgttgaaat ccgggatctg tatcaaaata
    18541 gaacaaacaa ccagtgggta gaaaaatatt ttccagatac cacgattatt gataaagaag
    18601 caaaattaag cgctttaggc aatcggctga aagacatcca agaagctatc caattcatac
    18661 aacaccacgg cgatagccgt caaaagaaac tccatttgaa acgtcaagaa ctttccttac
    18721 aagacttgga agcaaattat tccgaagaaa atttcatgaa taaattagaa gaagtccttg
    18781 atttaaaaga aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa gaagaggaag
    18841 aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga
    18901 ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactaa
    18961 gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt
    19021 cgtatgttgc tttaaaaaaa tcagttgctg ctatagaaga aattgctggg aaatacgggt
    19081 tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac
    19141 agcaattatc cattgttcac gagcagcaaa aaaagataac gacagcgtta ggtcagtgca
    19201 gcggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacggg
    19261 aaaaagtaaa agaacggttt atccatgcca cttatctgat cgttttgcaa ggttgggtag
    19321 atgcagaaga aaaacagaat ttgatgcatg tgttaaacga aactgtatca gaagaagata
    19381 tatacgttag tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat
    19441 tgaaaaacca tccgcttatc gaaccttttg aattgctgac tgaaatgtac agtctgccaa
    19501 aatatgaaga agtagatcca acgccttgga tgacaccatt ttatcttgtc ttcttcggga
    19561 tgatggtcgc agatgttggt tacggattgc tgatgctcat tgggacaata ctagctcaaa
    19621 aactatttgt tttaccccgc gggatgaaac gtttcgtaaa attctttgag attctctcgg
    19681 taccatcgat catctgggga ttgatctaca gttcattttt cggacaggca ttaccaaaag
    19741 aactatttgg tatccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga
    19801 ttttgatttt atccgtgatt ttcggtctga tacagatctt ggttggatta ttcgtggcag
    19861 caaaggaaaa tatcaggagg aaagattact tgagtgctgt cagtgatggt tttgcctggc
    19921 aagggatcct gattgggatc gttattgcat tgattggtgt catgttgata aaaaatacag
    19981 catttgttta tcttggcgcg gggctatcga tcattgctgc tttatgtatc ttaatcgtgc
    20041 cgattatcca atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat aatctttacg
    20101 gattgactgg ttatatcggt gatttagtca gttatacacg tttgatggca ctaggtattt
    20161 caggcggaag tattggcgcg gcattcaata tgctagttgc tttcatgccg ccagccgctc
    20221 gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac
    20281 tgttaagtgc ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt
    20341 atacaggagg cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca
    20401 atcataagaa aaaaaacaat aaataattgg aggattttta tacaatgatg gattacttaa
    20461 ttagtcaaaa tggtggaatg gtatttgcag tattagcgat ggcaacagcg acgatttttt
    20521 cagggattgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa
    20581 caagtcaacc cgaaaaattc ggacaggctt tgattctgca attgcttcca ggtacgcaag
    20641 ggttgtatgg attcgttatc gcctttttga tttttatcaa cttaggaaat gatatgtcag
    20701 ttgtacaagg attgaacttt ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt
    20761 caggtatcgc ccaagggaaa gttgcagcag caggtatcca aattttagcg aa
//
LOCUS       DACIRY010000043        20458 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.043, whole genome shotgun sequence.
ACCESSION   DACIRY010000043 DACIRY010000000
VERSION     DACIRY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.043"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(164..778)
                     /locus_tag="IYE22_001359"
     CDS             complement(164..778)
                     /locus_tag="IYE22_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9506578.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            922..1484
                     /locus_tag="IYE22_001360"
                     /pseudo
     CDS             922..1484
                     /locus_tag="IYE22_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1595..2569)
                     /gene="lacD"
                     /locus_tag="IYE22_001361"
     CDS             complement(1595..2569)
                     /gene="lacD"
                     /locus_tag="IYE22_001361"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ9506579.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(2588..3043)
                     /locus_tag="IYE22_001362"
     CDS             complement(2588..3043)
                     /locus_tag="IYE22_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9506580.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(3071..4516)
                     /locus_tag="IYE22_001363"
     CDS             complement(3071..4516)
                     /locus_tag="IYE22_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9506581.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(4513..5448)
                     /gene="pfkB"
                     /locus_tag="IYE22_001364"
     CDS             complement(4513..5448)
                     /gene="pfkB"
                     /locus_tag="IYE22_001364"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ9506582.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            5616..6374
                     /locus_tag="IYE22_001365"
     CDS             5616..6374
                     /locus_tag="IYE22_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9506583.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            complement(6869..8068)
                     /locus_tag="IYE22_001366"
     CDS             complement(6869..8068)
                     /locus_tag="IYE22_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9506584.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8511..9002)
                     /locus_tag="IYE22_001367"
     CDS             complement(8511..9002)
                     /locus_tag="IYE22_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506585.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            complement(9029..10174)
                     /gene="dgoD"
                     /locus_tag="IYE22_001368"
     CDS             complement(9029..10174)
                     /gene="dgoD"
                     /locus_tag="IYE22_001368"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ9506586.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            complement(10189..11556)
                     /locus_tag="IYE22_001369"
     CDS             complement(10189..11556)
                     /locus_tag="IYE22_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ9506587.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            complement(11581..11859)
                     /locus_tag="IYE22_001370"
     CDS             complement(11581..11859)
                     /locus_tag="IYE22_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9506588.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            complement(11878..12336)
                     /locus_tag="IYE22_001371"
     CDS             complement(11878..12336)
                     /locus_tag="IYE22_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9506589.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            complement(12348..12968)
                     /locus_tag="IYE22_001372"
     CDS             complement(12348..12968)
                     /locus_tag="IYE22_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ9506590.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            complement(12979..15021)
                     /locus_tag="IYE22_001373"
     CDS             complement(12979..15021)
                     /locus_tag="IYE22_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9506591.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGKDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            complement(15399..16538)
                     /locus_tag="IYE22_001374"
     CDS             complement(15399..16538)
                     /locus_tag="IYE22_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9506592.1"
                     /translation="MNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGS
                     YDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTLAKE
                     AIHIVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAG
                     AVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFA
                     EKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQ
                     NEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            complement(16535..17119)
                     /locus_tag="IYE22_001375"
     CDS             complement(16535..17119)
                     /locus_tag="IYE22_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ9506593.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFISKKAI"
     gene            complement(17309..20350)
                     /locus_tag="IYE22_001376"
     CDS             complement(17309..20350)
                     /locus_tag="IYE22_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ9506594.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
BASE COUNT         6208 a         4345 c         3393 g         6512 t
ORIGIN      
        1 tacaaaaata tttttattgt tggatacaga aatctatttt taactgggga tatctttgcc
       61 agctaatatc tttgagacta cacctatctt catctaaatg gatcaattac tcgctttttc
      121 cattgacctt cgtggtactt ctgctttctg atagtaaaaa acattactga atgatcttac
      181 tatataacat acagcggaga tctacttctt tattaaggat cttcatgacg ttatcttttt
      241 cttttttccg ttcgaaaccg cgtcgttcat aaaattgata cgtacagccg ctatccgtga
      301 acagaaatac ttcttttcct tgctctcggc gttcaaattc ttttaataat ttactaccga
      361 tccccttgat ctttaaatca gggttggcag ctaaaaagac aatttctcca tctggggaat
      421 gtttttttaa atagtctgaa agcaattcgt cattggtgtc gtcatacccg cctttgctgt
      481 ctttcacaaa tgcattttgt aagaaatcaa atagtttaac atataacttt tgactaaatg
      541 aatgatgctt ttttgatttt cctttgatgt ctgctaataa aacacctgcc aactcatcat
      601 ccgcatataa agcaatgatt tgtgtcgcgc gattcaattc tagataccaa aagtatcttc
      661 cataaagatt caaaaggagt ttgttcttgg tataccaatc aaagtgcata cctttaatcg
      721 cgaattgaat ggcttttttg tagtctttct tttccagttg cttaaaggct aattccatca
      781 cttattctcc tattctttaa atattgatat gctatactta tattataata gacaaggtgt
      841 cggttatcta tgttcagata gcggtgaaat tgtcggataa ccgacacaat tccttgatta
      901 tgttgattag gaggctgcaa aatgaaaaaa gaggatcgcc gcgtcaaaaa aacagaaaaa
      961 gcgttagcag aagcattatc aaaattatta gtagataaaa aaattcaaaa catcaccatc
     1021 cgtgaactga ccgaaactgc tgatcttcac cgttcgacat tttatacaca ttatacggat
     1081 atctatgatc tttatgaaaa actggaaaat agctttttcc aagacttgga tgaaatcatt
     1141 tcctacgata cgacacatag ctacgaggat ctatacgctc aattgatcga ctatgtctat
     1201 acgaatacgt tcgtatacaa gttattcgtc atcaaccacg gagatatcca atttcaaaag
     1261 agattaaata atctgatcga agaaaattat ttaaaaatct ggctacaaga agacaaaaaa
     1321 aaccgtgatc acagaagaaa tgcgttactt caccatctac catatccaag ggtgcctctc
     1381 cgttttaaat cattgggtag aaaagcaatt ttgcatactc taagaaaaaa atgctgacct
     1441 tcttgcgctt attgaacgac aacatagaac ctatcatgcc ttaaaaacaa acgaaaaagg
     1501 aagcgtcggg acgcttccaa ttctttattt aaaagctcaa attgattatt aaaaatcatt
     1561 aggttacaaa cttaaggtta tagttaaagc tttcttacaa tttaatagct gttgctgttt
     1621 ctttcaaaac attattcagc tcttcgatgt tcttgcgtcc ttgtgtctgc atccattctt
     1681 tggcagcata ttcaccttta gtaacgaatg gttctacacc attcgcccaa gttgcgcgcc
     1741 cacagagtac cccattgaat gtagagccag cctcttttgc aaatttcaag gtttcttgga
     1801 acaattcagc agttactcct gcgctcaaga aaataaatgg caggttggta gtctcggatt
     1861 gttctttgaa ataagcagca gcttcttctt tggtataagc aacttcattt ttagtgtaac
     1921 catccacata gttcatatta acaggaactt ccactttcaa aacgtcaact ccatagcgag
     1981 gcttagaaaa ttccttcatc atttcattta ctttatgcgg ttttttcaag gcatattcct
     2041 tcgaagaagt atcaggatcg ttggcatcat aagaaaccaa ttctaagaaa aatggcaaat
     2101 cttcagcaac acattcagaa ccaatccgct ccataaacgc cttcttttgg tcattgatgt
     2161 catcagattc atctacatcg tagtataaca agaacttaca agcatcggcc ccttgctcct
     2221 ttagacgttt tacagaccaa atagataaaa tatccggtaa acgtcctgga gttgaagcat
     2281 cgtaaccagt tttttcatag gccaacaaca gaccagcatt tttatcgcga acttcggctg
     2341 ccggcaaccc atattctgga tctaataaga ttgctgaagc atatggagtc aattcagaag
     2401 aaatgatttt tttgaaattg atgatttgtt catcggttgc tgatttattt tgaaatttac
     2461 cgatcatttt tttcaaggca ccacgttgat cgattgctaa cgcactgatg atgccacttt
     2521 cattacttag ttgacgaata taagttttct ttttttctgt taaaggcatg atttcactac
     2581 ttccttttta gttttcttct aatttttggt ttaaaagttc agctatttct tcagcagact
     2641 gggcttgttt cagaccttct gtcacatctt tgtgcatcaa aagtctggct acctgagata
     2701 aaaattttag atgggttgtt ccagcctctg aatctggaat aaacagagcg attacaaaat
     2761 ccgttccttc tccatctaaa cttttccaat cgaccttacc gtttaaccga acaatcacaa
     2821 tatcggcatc cttgatacta gcatctttgg catgagggat ggcaaaacca tccatcatac
     2881 cagtcgtgct ttcttcttct ctttccatca atttttcgta aactgcatct cggtcttcac
     2941 taatgctcaa gacaattgac tgatctgcaa ggaagttcat tacctcatct tgcgtcgtaa
     3001 aatttttatt cataaaaact ttatttgttt tgatcaaagt catttgaaat ccttctttct
     3061 agaataacct ttagttgttg gttgcttctt tcatttcaac agcagctgct tctgcttcac
     3121 gtttttctac actacgtttt tggatcaacc ctaaaattac tccgccaacg attgaaccaa
     3181 gtaaaatcga ccctacccat gcaaccttac cagtaacaac aggtaatact agaaaacctc
     3241 catgaggtgc gggaacttgg acaccaaaca tatatgtcag tacagcagca attgatgaac
     3301 ctagcatcat agttggcaga acacgcagcg gatctttagc cgcaaatgga attgctcctt
     3361 cggtaatgtg cgttgagccc aagataaagt tgactaaacc agcgttgcga tcattcgtgt
     3421 cgaaatattt tccaaaaaac agtgcggcaa atccagtaat caatgggggc gcgatacaag
     3481 ctgccgaaac ccctgccata aatgtatggt taccttgtgc caacaacgca gtccctgtaa
     3541 cataagccgc tttattgact ggacctccca tatcaaatgc tgacatacag ccaacgataa
     3601 tccctaaaat taatggatta gaattttgga atcctgccaa gaaattcatc attccttggt
     3661 tgactgcttc cattggattt gctaataaag acatgactac accaacaaac aagacaccga
     3721 tcaccggata taagaagatc gccttcagac catccaattg tttaggcaag cctttcatga
     3781 ccttttgcaa tagtaagata aagtagcctg ctaagaaacc tgcaaaaatt ccgcctaaga
     3841 aaccggctcc gccagaattg gctaacagtc cgccaacaaa cccaacaacc aaaccaggac
     3901 gtttagcgat tccttcagcg atataagctg ctaaaatagg aaccatcatt cccattgccg
     3961 catcaccaac accctttaac atagcggcag tcggattgta agtaggatca ttgggatcaa
     4021 aagagtacac accccagatg gcaaacgaaa ttgcaataat taccccacct gccactacaa
     4081 acggcagcat atgagaaaca ccgttcatga gatttttata aatactgtga ccaatgcctg
     4141 ctgcaacccc catctccgct ttctcaccag cagcgtcaac tgttttgttt tctgcagtga
     4201 ttgaaccttt accagccaac gcatcttcga tcaattggtc agcatcttta atccccttgc
     4261 tgacaggcac atcgattaca cgcttacctg cgaaacgttc tgcttgaaca tctttgtcag
     4321 ctgcaatgat tacagcgtca gcgtttttta tttcttctgg tgttaattca ttctctatcc
     4381 ctacttgacc atgggtttca actttgatag ataccccttt gcgcttagct gcctgctcca
     4441 atgcttcttg agccatataa gtgtgggcaa ttcctgttgg acatcctgtt gcggcaacaa
     4501 ttttgtaagt tgtcattttt tactcgctcc ctctttgatt tcgatttgtt tttccaactc
     4561 tgcaacatcc gaaaaatctg ttaatccttt acgaaaagcg gttgaacttc cagctgctaa
     4621 acttttcttc aaattaatat ctacctgttc accttttaaa tatcctgcaa gaaatgttcc
     4681 caacaaagtg tcaccagcac acgctgtatt gactacttta cctttaggag aattcccaga
     4741 ataaacgata tccttattta aaaatattcc tccctctcct cctagagaaa ggagaacatt
     4801 tttcgctcca tcggcaatca agcgttcagc ataatgaata tatccttcac gatcactaag
     4861 ctcgacatta taccaagatt gcaattcctc ctcgtttggt tttaataaga acggctgata
     4921 cggcagacaa tcaattattt ccggatagct tgaatcaata atcagatgga ccttattttc
     4981 aaaacaaata cgactgatat caataagaat ttcgggtgag acccctcgcg gcaaactgcc
     5041 agaaacacac aaataatcgt cagcttgcag cttttgtatt tgatttagaa aagttttgat
     5101 ttgttcctga ggaatctcag gtcctttatt aaccaatttg aattcttcat tggttttatt
     5161 aacttgtgta aaaacgttga ttctagtcat tcccggtacg ctaataaaat cagtgctaat
     5221 atctttttga tgcaaataat cctcaatata attcccggta aaacctgctt taaatcctaa
     5281 agctgtacta tcgataccta attgcttcat gatcaatgaa acattcacac cttttccatt
     5341 agcttgtata tcatcatcgt ttgttcgatt gacttgggat ggtttcattt catctgtttc
     5401 gataaaaagg tcgattgcca agtttaaagt acatgtataa actgacattt acgctcctcc
     5461 tttcttacat attcattatg tatcaagatc gaatgtaaat gttttcataa atgactaaat
     5521 tcattgaaaa cttttacaaa atatattaaa taaacttgaa gtaaaggaaa ctcccgcttt
     5581 acaatagaat tatcctaact aatcggaggc aaaatatgga tacaaaatta agtgaatcgg
     5641 agaaatatct ctgggatttt attcaaagtc acatactcga aatcccctcc tattccattg
     5701 taaaactaag tgaatatgcc aacgtttcaa cagcaaccgt cgttcgtact atgaagaaaa
     5761 aaggatatga ggggtttacg tcttttaaac attcgttaaa agaaaaagaa aatacaaata
     5821 tcaattttgc cgctcttgat aaagttgatg aaggaataaa gactgctatt cttaaaaatg
     5881 agcaagaggt aatccgcacc atcaatatgg tagaaagcgg gaatattgaa gatgctattc
     5941 aacgaatcaa ggctgctaga agaatcatga tttttgcccg aggtttttca gaattgattg
     6001 ctcaggaaat gatggtcaaa tttcaactta ccggtaaata ttgtgagatg catactgatc
     6061 ccaatatcat aaaaaacatt agtagaaaat tatccaaaga tgacatcgtg atctttgttt
     6121 cactaaacgg tgaaacaaag gaattgacta ccgcagctga aaactgttat aaagctgaga
     6181 tcgggacaat tttgcttact gctaatcgtc attcaagttt gatggaacat atcgagattc
     6241 cttttgttgg ctttaaatca gaaggctcct tcttcccaga ttatgaggta cgctccagac
     6301 ttccactttc tatactggct agaatcttac ttgactccta tgcactgcga acatcaggat
     6361 tcacacagca ctaactctat atctgtaaga ctgtacctaa aatatatctg tgtgaattaa
     6421 ctaaggttgt ctagaaaaat atttaatcgc tatttgtatt ctcgtgcttt ctatcattgc
     6481 tccttctata acttcttagt ttcaagatag aatagtgcat tgagactacc aggactactt
     6541 acgcaaatat ataataataa gaatgtgata agcagttcga agttcctgtc taatatttct
     6601 tgttaattat tatcaatttc caacaattct agaattataa tacgcaaaaa aaggagaccg
     6661 tctaaccgtc tccttatagt ttattattca gattacaatc agttatctaa aatgttacag
     6721 aagttttatt agggatattt tcttatcttt ttagatgagg tttctcaatt atttagaaat
     6781 gcttacttaa gttattctcg ttttttgtaa ttacgccaat aatcactata tgttatttat
     6841 ataaaactag tatctaagta tttttatgct actcaacaag agaatcacta tcgtttcctg
     6901 tcttcggtat ttttcgaatg cccaaaatag ttaataaagc acctagcacc gcaaaaccag
     6961 taccgaaaat cagcatctcc catacaccga aatgctgcag cagactgccg cccaagacat
     7021 ttcctatcac cccaccaaga gttgtggcga tgatgatcat cgtttgtcca aatacacgat
     7081 catcatccgc cagaaattga ttgaacaaat aagccgaagc tggaataaac aaagcgaaac
     7141 tgaaggcttg taggagatgc gtcatttcca tcatcaggat cgaactggcc agcagcatca
     7201 tgaatcctct gacagcaaat gaaatactgg caacagacag ccaaaatttg ctggatttct
     7261 ttttgacaag ctgttcaaaa aagatcattc ccggtaattc gcataaagcg gcgatcatca
     7321 atgcgatgcc aacttcttta ctgccgccgt ggatattttc aaagatctgt gccaaaaagc
     7381 tgttgatcat ggtgtggaaa gtaaaaagaa aactgaaacc taaaaataac aaaatcagga
     7441 aaggatattt ttttctcaaa gaaactgagc gatcctgttc ttttgtcata ggaaaaactt
     7501 cttgattttt tacatccggc agcagatgca aaataacgaa aaatatcact gcttcaacta
     7561 taaccaagat tggcaaaatc gcggcagaat aaatcgctaa cagctgtccc agtaaaaaag
     7621 aagttacagc ataagaaaat gaaccaccag ctcttgaaaa gccaaagttg atcctgtagc
     7681 cggtatttga atactcaaat gccagcgcat tgagaaatga ttgcatagaa actactaaaa
     7741 tcccacccaa gatataagta atgatcggca aattcggaat caatacccca gctaagagaa
     7801 tcactcctaa tcctgctatc atcgtcttca gcacggtgat cagtgggatc tttcttttct
     7861 tgatgatcac ttgactgaca aaaggctgaa gcaccgttga aacaatattg accaatgcta
     7921 agatccctcc gattttagct ggatcaatct tgaaataaat caaaaacaat gtcgcaaacc
     7981 catagacaga acaaaacatc atccaataaa gaaaatgcaa acttgtataa taaaaacgaa
     8041 tcatttttcc gcttgtttga tcgcgcacac atctcccacc ttattggttt ttctttagca
     8101 atttactata gcatacttgt aggggtttta ctatttttaa aagtggtgaa gaactatgga
     8161 aattagggta gattacaata tgcgttgatc aattaaaaca tctgttaata aagacggaaa
     8221 aaagatcata gaagcatcgt acttttttag catactagct tggtcatttt tttctggaat
     8281 tgaagaaaat atatatattc taaaaactat ccatcatctc ttagattacg agcttttttt
     8341 gctgcaaagg aagttaaaaa aagcagggat cgagtaatcg attcctgcta tatcttgtac
     8401 caatatattt ctttccaaaa cttaatatac attgtatact tcaatcaata tgattcatta
     8461 aaacaagttt cattattcgt gataaagaaa tcataatttc ggtagtagaa tcattcatta
     8521 tcttttttta caactgctct tttttcttcc gaactttgat ctaaagtaat tttggaatgg
     8581 atatcaagtt cttgaaagac atccatcatt tcagtttgat ctttatcaaa aatcattgaa
     8641 aaagaattat tcttgctttt gtaaaatgaa ataaatgatt tatcgtcacc tagtttttct
     8701 acttggactt gaggatatcg attaaagtta ccgttcaata ctccaaatct aacaacagaa
     8761 tcttttgcta gaccctcctt caaaaaacca ttgatcaaaa tgacagaagc aaaagcaacc
     8821 agtttaatct gatcagtcag atgttccggc cagaaaatca acaggatggc gactgtcagt
     8881 aaaatactca aactcttcct tacaggacta ctctgagcaa acacgataat tttcttttga
     8941 tcgattattt gcaaaataat cagcagtcct aaaaaaactg tgatcataag tgcttctgcc
     9001 atctttcatc atcctttatt ttcatttatc accactcagc gatcgtaccg tcataatttt
     9061 tccattgcgg attggtccaa atcaaacctt cttgtgcaat ttctttgatt ttgtcttcat
     9121 ccatttccaa gcccagacct ggttttgaag gtagatctac gtagccgtct ttatattgga
     9181 aaacttcttt gttcttcaca aaatctaaca gatcaaaacc tttgttatag tggatcccta
     9241 agctttgttc ttggataaag acgtttggcg tacatgagtc tacttgcaga gttgctgcta
     9301 gtgcaacagg accatatggc gcatgaggcg caacagccat gtcgtaagct tccgccatcg
     9361 ctgcgatttt acgggtttcc aagattcccc cacaaagagc cacatctggt tggataatat
     9421 caacagtacc ttgtttaaag aggttcttga attgccaacg agtgtacaag cgttcaccag
     9481 tagctaatgg tacagccaca gaatcagcta cttctttaaa gtgttcttca ttttcaggta
     9541 aaacgacttc ttccaaaaac attggatgga atggctccaa agcttttgct agcacttttg
     9601 ccatcggttt atggacccgt ccatgaaaat caacaccaat atccattgca tcaccaaaac
     9661 gttcgcgaat agacgcaact cgttccacca cttcatcaac tttttggtag gaatcgatat
     9721 aatgcagctc ttctgtcgcg ttcatcttga cggccgtaaa gccccgatca aaccgatctt
     9781 gtgcttgttc tgcaacttca cttggtcgat ctccaccaat ccaagaataa accttgattt
     9841 tatcacgtgc tgcaccgccc aataattcat aaaccggaac attaaacgct tttcctttga
     9901 tatcccacag agccatttct aaacccgaaa cgatggttcc attaatagga ccgcctcgga
     9961 agaaagatcg atgcatttct tggaagaggc gctcgatttc aaacggatta cgtccaatca
    10021 gccggtttcc tatctcataa gctccagcaa caaccgtttc tgttttagtt cctgagatca
    10081 tttctcccca gccttcgatt ccttcgtcag tcgagatctt aacaaaaatc catcttggtt
    10141 ttactttata cactgtgaca tctgaaattt tcaatttaaa ccctcctact attgtgcctt
    10201 caatggttca tccatttcat gattaacttt tttgatacca tcgaagtaat accatgcgcc
    10261 cgcaaaggct aataacaaca ctggaatacc gatgattggt ttgtaagcaa atgcgataaa
    10321 taccaaataa ctttggatca tggaagtcgc agcaaatgat gagattaaca ttgaagaagt
    10381 tcctaagtta ataccaaccg atttggcaat tgctgtcagc attggcgcag tagctgaccc
    10441 acaccatagc aatgaagctg tcacaactaa gccaataatg atatttttta ataaattacc
    10501 acgagtcaag gcaacgacca ttgccacgcg gaaaggaaca accgctaaat ctgcgaatgg
    10561 taacacacgg tttcctggca atactaaagc taatgcaatc gttagtggaa taatgatcaa
    10621 tgctgttgtg ataacatctg gattacctac aacaactgca gcatccaatc cgataaataa
    10681 acgtgtacct ttgaattttt tttgtgacca tttttgtgca gcatctgaaa ttggcatcaa
    10741 tccttccata aataatgaag tcatcttagg aatcaaaacc aaaacagctg acatgctgac
    10801 acctaattgt aaaaccgctt tgatatcata gccggctaaa acaccaatca ccatcccaat
    10861 aactaacccc atgatcatag aatcaccaaa aaatcccaaa tatttttgtg catctttgat
    10921 ggtgaatttt gattttctga ataacgggat acgatccagc agccaattta acggccaagc
    10981 aataaccagt gatgaaagcg cagaaacagt tggtaaagaa acaccaggga ttccgaagaa
    11041 actttctacc atcggttgcg accagtctga aatcttaaaa gtcgcaattg ccatcactac
    11101 tgatgcacct acacctaaga agacattctt agtcacaaaa tacactataa caccaacgat
    11161 tgccatatgg tgatagttcc agatatcaac atccagcgta tgtgtacgtt tcaaaatcag
    11221 caaaacgata ttgaccaata gaatggcaaa aatcaagatt ggaatgattg gcgatgccca
    11281 agtaacagcc gcaatcgaac cccagccaac atccaaagca tctagtttaa caccagttcg
    11341 atcaaccatc gccttggcag ctggtccaac attggtcgtc ataaagtcca atatcaactt
    11401 gatcccggca aaaccgatac ctaatgtcaa acctgattta aaagcacgag aaatctttaa
    11461 cccaaaaatc aaaccaataa tcgtgatgat gactggcaac ataactgttg gtccagcatc
    11521 aataaagaag ttaaagacgt tcataatgat tttcattttt tcagctcctt tgatcagcta
    11581 ttatttcagt aagttcaaaa tctcttccat tgtctgatcg gcaccgatac ctgttaaaat
    11641 cggcatccct ttgattactg gtacaccacc tgtttgacca tcaaattgac cagtcgtcac
    11701 caatagatca tagtcttgaa cttttcctgg aacttccatc aattttgttt gagtcgttga
    11761 aacatcaatg ccatgttgtt gcaaatactc tctaactttt gttgctacta ctgttgaagt
    11821 ggcgatacca tttccgcatg ctactaaaat tcgtttcatt atgatttcct ccttatatta
    11881 aaaaaccgct ttctttaata gttctaataa ttcagtatca ttgcctgctc tgatcatttc
    11941 ttgccgcagt ggctcatctt gtatgattcc aacaattttt tgtaacattt caacttgccc
    12001 atgtggttcc ccaatcgcca tcatgatcac aatttggatt ggtaactgct cattgttatc
    12061 ttccatatta tgaaatagga ccggatcttt aagagtcgca attgctaaag ttgttttatt
    12121 gaccatttca tagtttgcat ggggaattgc gattcctggt ggtgttgatg gtaatccagt
    12181 agggtattct ttttcccgtt cttggattgc ttgaatatat tcctccttca cgtagttttt
    12241 tttgtacatc tcgcttgcta aaaacgctaa gacttcacta tctgtttttg cttgaacatt
    12301 tcgaaaaacg agttcctcat tcaaataaag ttccaaagtc ctcactccta tttaatagcc
    12361 tcaattaatt ctttcatcga acgtgctaag tttttaacat ttagctcttc gatgtcttgc
    12421 ttgttgaaca taccggaacc aagacctaaa tagctagcac cttgttcaaa aaactctttc
    12481 gcattctcct tactgattcc accaaccccc attagtggta acttacctaa aggtgcttga
    12541 atatccttga aaaaacgtgg agtaacaacc gctgcaggaa atacttttac aatatcagct
    12601 ccttctttaa acatacggtc gacttctgaa ggagtcattg cggcaggtac cgccaataat
    12661 cctttcttat tggcgtaaga caacatttct tttgtgaaca tatgtggtcc taataaaaat
    12721 tttgcaccag cctcatagca ggcttgggct tgttctaatg tccttaccgt cccagcgccg
    12781 atataagtcg tgtcaccata acgttgattt agttcatgta tattttcaag agctcctttt
    12841 gtgttcattg tcatctctac ggcaaattga tggtcaaatt cctgcattgc ttgtaaaata
    12901 gcatctgcct gatcaaaagt ataaccacgc atgatcactg tcaatttagg ataatctgct
    12961 agtctcatgt tttcctcctt aagatctttg tcttaatagt tgataaactg tcttttcgtc
    13021 atcagcaatc aataactgct caatcaatga atcatctttg gcgatatcag cgatttgatt
    13081 gatggctcgc agatgcttcg ttaaatcaaa aaatgaaagc ggcaaaatca agctgacatc
    13141 tttaccatta ggaaatcgta tcggcttttt acaaactaaa atgctgacac cgtcactgat
    13201 tacaccttta tcgaccgttg tatggggaat gcaagtcttc attcccaagt aacctgcata
    13261 tcccggttgt ctgatttgat ccaagcaatc agcgataaaa cgcgagtcaa tgatttgatg
    13321 ttccaataac ggtcggcaag ccacctccac tgctcgttcc caagacatct cttcttcaac
    13381 gatttgtaca taactcggcc gcaagtaata agtcaatgaa ggcaatacat tgaagttgtc
    13441 cagcggcgtg cttccatctt ccttgatcaa aaagtactgc agttcttctc gcaacgaaac
    13501 tggattggtg atatttgcat atttcctaat gattgttaac aattcatcca ttgctcgatc
    13561 aaccttagta attcctaaat cactcaaaac tcgatatcgt aaactgattt gttgctgata
    13621 tgtcatgatt ggctcgatca taaactgcag catagaactt tttagtggcg tcgtagtaaa
    13681 gaccagatca taatcagctt caaatttgta gaaatcacgc acagataggg aagctaaaaa
    13741 atggatctct ggaaaaagtt tttccatatt ggctcgtact aactttgaga ccatgacacc
    13801 attagtacaa acaactacag ccctttgtcg ttgcttattc aatttattat gattacttaa
    13861 ttgatagcca aagtaatagg ataaaagatc taattcatca tttggaaagg ccttatttag
    13921 ccaattctca ataggaataa tcaattcttt catcaaatca atcaaaatcg catgattact
    13981 atccttgatc agactatcta ttgaataaac tcctaaactc aagttatagc gtattcgaaa
    14041 acaggctgga cgcagatgac tcagtatcct acgttcaaag tcttctcggt catcgatcaa
    14101 aatcaacgtt tgattttgga aattttcaac catttggctg atcaattttc gcaattcttg
    14161 atccgaatca taattttgag tagttttgct ctcaaaaata ttggaagtta aaaataataa
    14221 cgtgaaccac tccatataac ttttggatag actccaacct gtttcatgta ccaagatcat
    14281 cagcattcgg aattcaggag tatccgtcac atcatcggga aaaaacgcgt gcttatctga
    14341 catcttgatc ccacggctta ctaaaaatct cgctgatgtc tgcaaatagt ctaccgactc
    14401 atcagaataa ctaagatgca acatttgttc catgttatgg atcaaatgga cgatttcttc
    14461 ttcagaaaca tcttgtgcca attttctgcg aatcatttct cggcgaaaaa tcggaaaccg
    14521 ttttactaga tccgataaca gctgtaagat acggtgttcc gaaccaacca attggtaacc
    14581 attcatccga ttatatcgaa ttgtcagact ataatttctg atgaacggtt ccgttcgttt
    14641 gatatcttcc ataacagtcg tcttactaac atcaaccgca tctaccaaat gatccagact
    14701 gatgtaatcg atattagcga tcagagtcat cactatcaat gcttgtcttt cactatcagt
    14761 gagaacgaca agttcctgat catcacctat gtcgtaatca ggctgtgata tcatttggat
    14821 aacctcgatt ggtaccgtaa tatttccaga atgacttctc tgaattaacg gaatgttctt
    14881 tgttttcaat tcttcattaa attgattgat cgcgtaattg atttgacgac gtgtcaaatc
    14941 aagttcactt gttaaagtgg aaagtttcat ttcagggttt ttaataatca gtttgagtat
    15001 ttcacgaatt cgtttattca ttttgactat cctcctgaca aaatcattat atacttgttc
    15061 acatacgttt ccggaaagct ttcatcacgt tttttgtcac acttggttta atgttttgtc
    15121 acactaacag ctatttttaa aatgaacgct gttatatcaa atgtaacatg gtatgacaat
    15181 tattttaggg ctaatgtgaa tattaaatat tcggtagaat attggatgct ttagtaaata
    15241 aacacgggac gttgcttctt caacacatta aagtatctat tccgtaacta aatcaagtca
    15301 gtctaattga aaaagaataa tattaaactg ctaaaaacaa tgcttttggg agcgtcaata
    15361 attttgtgta aatggttctt ccctactgta gattgtttct aattctccat cggttgactc
    15421 agcatttgtt cgagctcgcc ttcggcctgt tggaaacctt tatgactgcg acctaggaat
    15481 ttttggttgt aagtatcaaa agacgagact aagaaacgtt ccatcgactc ttcattttgg
    15541 aactgttcct tgcggtggct gtatttcttg atttgcttat taaaggattc aatcaagttc
    15601 gtagagtaaa tagtcttgcg tatggctaat gggaaatcat agaaagtgag caggtaatcg
    15661 ttcgaaagga tcgattccac catttttgga taactggttt tccatttttc cgcaaaagca
    15721 ccacgtgctt ccaatgccgc ctttttagat gacgcttgat acaccatttt aaaatcatca
    15781 cagacttcct tacgatcatc gacacgcact ttgtgactga tattacggga aacgtgcaca
    15841 cagcaatgtt gaaaacgggc tttggggtag aaccgctgca cggcccctgc catccccttt
    15901 aagccatccg tgatgaaaag gaggacgttt ttcacgccac gttcctggag gtctattaaa
    15961 atctcttccc aaatcgtgat agattcagtt ggtgcaatcg catagctcaa cacttccttt
    16021 gaaccgtccg ggcgaatgcc aactacgata tgaatggctt ccttggcgag ggttttccgc
    16081 tttaaaggga tataggttgc gtccatataa atagcggcat aacggtcatg aagctcacgt
    16141 tccttaaatg aagtgacttc ttccgtaaat gatttagtca tattggacat ggtttggggc
    16201 gtgtagtgat gcccatacat tttctcaatc aagtctgcga tttccgacat ggtaatacct
    16261 tttcggaaga ggtgaatgac ggtctcctct aaggtatcat tcgtccgtct ataagcagga
    16321 acagtctgtt gcttgaactc gccgttgcgg tcgcgcggaa tctggagatg aagttccccg
    16381 tactcggtct tgaccgtacg gtcataggag ccgttacgag aattacccgt gttaaaacca
    16441 atgcgatcgt acttttcgta atcgaggaaa gccgttaact ccgttttgag aagcgtattg
    16501 acagcttttt ccaaatggac acggaataat tcattcaaat cgccttttta ctgataaaac
    16561 gataaaatga tagtggttcg tttgttgcat actcatttaa tagatgttga attaaaatat
    16621 agttcttcga ataattactt tttgatttaa catctgcttc accacttact agaatactag
    16681 caaattcttc attcccctca tcccccatta ctcgagacat aatcagaatt ttatctggtt
    16741 taacctcacc tgttaaatca tcttgttccc ataacgcggg ttcaatttgg cttttcaaga
    16801 ataaagcttc cttttcacaa ctattttcta atttagcata gatagctctc aaaaataaaa
    16861 atagcgtagt tatcctttgc tgtccatcaa tgatttcttg ctcgttatga tcattttcat
    16921 aagccacaat gcttcctaaa aagtaagtac tctcgttatt gtttgctgtg tactccgcta
    16981 aatcatcaaa tagtacttgg ttttgctcgt cagtccaggc ataaggacgt tgatattctg
    17041 gaataacgaa ttttttgatt ttccctgttt caagaaactg cttcacgctt tgtttgttca
    17101 cttcaattgt cgttgacata gtttaaatct ccgagacgac actgtttata aaaataaatc
    17161 tatttccttt attctgacat agctgatatg acagagttca gacaatttag tacgtaacaa
    17221 atttttcagg gctttcttaa aagcaaacaa tacaacttct cctttatttt tgagagaaat
    17281 tgtattgtct aatccttatt tataaatttt atttcgtccg taataacttg tataaatctc
    17341 tagcaactaa tgtatatact agatcaaagt acttatcatc atgcccaaac attttctcaa
    17401 aagctgcatt gttcttctcg tactgctcca tcaccttatc tttaaataca tcagggaaca
    17461 gcgatttttc aaacatctga gcatcgttat tcttagccat gctgacaacc ttcaaatctg
    17521 ggttatcggc aaatgaccga tacaaaatct ctactaacac ccggtctcct tcgtcaaaat
    17581 catttggaaa tcgttcattg attttatgaa tgatttcttc tagtgaatct cgttcatccg
    17641 gcggtttgat tcctgcatca attgactttg gatttactag ctcgccttct ttattcacta
    17701 gatggatttc accggaaaag tcctgacgta acttataata ttccaacttt actttgtcag
    17761 aaatatcaat attttctcgt gtttctttag gtagaaactt cagcaagtat ccagtaaaaa
    17821 ttcgttcctc caatagttcc atatcgaata accgatcaag ttgtgaaata taatcatacc
    17881 acttgccaaa gttacgtaga gtcacccgaa actggtactg attatccttg ttcatttcct
    17941 catagcgatc cataatcggc ttaaacatgc tagaaattcg tccaagcaag cgctcattct
    18001 gttgcttttg ggcttgtttc accaatttca tgacacgttc aatatcacct tgattgtaaa
    18061 cattaaattt tcgtagcttg ctttgagtat cataaatcaa gttaacattg atttcttctc
    18121 tcaaatttgt ttgttcataa aagggttgga aagcagcttc aatatcttca acatcattta
    18181 caaaatcaag aataaaggta tccttcttcc caggcattgt tcgattgagt cgtgataaag
    18241 tctgaacagc tttaacaccc tttaacttct tatcgacaaa catggtatgc agcaacggtt
    18301 catcaaaacc cgtttgatat ttttcagcaa caatcaagat attaaaatta tctgtatgga
    18361 aagcttcctt taattgattc tcccgaacat tctcaccaga ttttgtgaca ttcatcttcg
    18421 tttctgtata ttcagtctct ccatctacta ccgaaccaga aaaagcaacc aacacatcca
    18481 agtcattata acctttgttc gctatatatt ttttaaattc tttcatatag cgaacagcgt
    18541 gtaagcgtga tgcggtaacg accattgctt ttgcctgccc accaatagct gtacgagtaa
    18601 ccgctcggaa ttgctcaacc atcacagccg tcttttgagt aactgcccaa ggatgcaatg
    18661 attggaaacg aacaatcgct cgggttgctt cactctttgg taattctggg ttatcttcaa
    18721 tttcacgggc aattttgtag gaagtactat aggtcatata gttcttcagc acatctaaga
    18781 taaagccttc ctcaatcgcc tggcgcatcg agtaaacatg aaagggttcg aaatgcccat
    18841 ctggttgttt cgtaccaaac atttccaatg tcttttcttt cggtgtagcg gtaaaagcaa
    18901 agaaacttaa attttgatgt ttaccttgtg ctaccaattg ttgaactagt tcatcttcag
    18961 catctggtaa tacatcgtca acttcgcctt ccagttcata atactcttcc aaggatttag
    19021 atttgtccga aagagcggct tttagcttct gggcactgtt cccagtttgt gaagaatgtg
    19081 cctcatccac aatcacagca aaattacgac cttccacttg atctacttcc tgatagatca
    19141 ctgggaactt ttgcaaagtg gtaataatga tcttacgccc atcattaatg gcttctttta
    19201 aatcttgaga ggatttgcga tcgtcaatcg tttctaccaa acctgtctga tgatcaaagt
    19261 tcatgatagt attttgcaac tggcgatcca acactgttcg atcagtcaca ataataattg
    19321 atgaaaaaat tgggtagttc agttgatcat gaatagaggc taaatggtat gccagccatg
    19381 caatactatt agattttcca gaaccagcac tatgctggat caaataattt tctccactac
    19441 catggctttt tacatctgca accaattttc gaaccacatc taactgatga taacgaggaa
    19501 aaatcaacgt tttctttgtg acttctctgt cgccttgctt ttctttttta atctctagat
    19561 tcataaaacg ttggataata tccatcaaat tgtctctagt aagcaccttt tcccacaagt
    19621 aggcagttgg gtagccatct ttattggacg gattgcctgc tccaccaact cttcccggcc
    19681 cattggaacc ttgattgaat ggcaagaagt acgttttttt accgtccaac tttgtcgtca
    19741 tataaacatc tgtatggtcc accgcaaaat agactaagaa gcggcgatta aaacgaaata
    19801 ccaattcttt tggatcacga tctttttgaa actggagttt ggcattttct actgtttgac
    19861 cagtgtactg gtttttcaat tctaaagcaa cgagcggaat accatttaac gacaaaacca
    19921 tatcaatggt tttatggtta ttaggactgt aagcaaactg gcgagtacag gtaaaactat
    19981 ttgcttcata cttttccatc gtttcatcat tcagctctga agcaggtttg aagtaagcta
    20041 ctttaaagcg cacaccgcgg tctttgatcc cattgcgcaa tacatgtaac atgccgtggg
    20101 tttctacctc ttgattaaag cgttgaagga acttgacttc tccatcattg ttatagtttc
    20161 gttggtgacg tgcccactgt tttggttgag tcttctggac aaagcctagc aatttttgac
    20221 aatccaaacc aattatggga tcaaagtttg attcattgaa tgtttctttc ttccatttgc
    20281 catacgtagt cagtgaagtc tcaatatcct gctcaaaacg aatttctttg ttctctacca
    20341 aatgaatcac ctcctaggct tcttttttac cggttacgta ttcgtagatg agagcttttt
    20401 tataactctc aagttccatc aattcagcta tcttattatc aatcatttga tttattat
//
LOCUS       DACIRY010000044        20342 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.044, whole genome shotgun sequence.
ACCESSION   DACIRY010000044 DACIRY010000000
VERSION     DACIRY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.044"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            88..1275
                     /locus_tag="IYE22_001377"
     CDS             88..1275
                     /locus_tag="IYE22_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ9506595.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(1468..1698)
                     /locus_tag="IYE22_001378"
     CDS             complement(1468..1698)
                     /locus_tag="IYE22_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506596.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(2134..2700)
                     /locus_tag="IYE22_001379"
     CDS             complement(2134..2700)
                     /locus_tag="IYE22_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ9506597.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(2700..3656)
                     /locus_tag="IYE22_001380"
     CDS             complement(2700..3656)
                     /locus_tag="IYE22_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ9506598.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(3656..4312)
                     /locus_tag="IYE22_001381"
     CDS             complement(3656..4312)
                     /locus_tag="IYE22_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ9506599.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            4682..6700
                     /locus_tag="IYE22_001382"
     CDS             4682..6700
                     /locus_tag="IYE22_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9506600.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            7127..9541
                     /locus_tag="IYE22_001383"
     CDS             7127..9541
                     /locus_tag="IYE22_001383"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ9506601.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            9889..11442
                     /locus_tag="IYE22_001384"
     CDS             9889..11442
                     /locus_tag="IYE22_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ9506602.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(11823..13607)
                     /locus_tag="IYE22_001385"
     CDS             complement(11823..13607)
                     /locus_tag="IYE22_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506603.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(13622..15343)
                     /locus_tag="IYE22_001386"
     CDS             complement(13622..15343)
                     /locus_tag="IYE22_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506604.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            15443..16204
                     /locus_tag="IYE22_001387"
     CDS             15443..16204
                     /locus_tag="IYE22_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9506605.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            17171..17439
                     /locus_tag="IYE22_001388"
                     /pseudo
     CDS             17171..17439
                     /locus_tag="IYE22_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            18111..>20342
                     /locus_tag="IYE22_001389"
     CDS             18111..>20342
                     /locus_tag="IYE22_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506606.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6663 a         3606 c         4101 g         5972 t
ORIGIN      
        1 taaaagtgaa gcatcaaaaa agccattgat ctcagttagg agtacaatgt tttttgcaga
       61 aaacatactc gaataggact gatatcaatg actcaagtac attttacttt tgaaagcgaa
      121 gaaattcaag caattataaa cgaaagcggt gcgaatgata ccgcaaaaac acttatgact
      181 atcatgttta atcagctgat ggaagaacaa cgaaatcaat atattcaggc aaatgcctat
      241 gaacgttcag aagaacggca atcacaaaga aatggttact acgatcgttc gtttacaact
      301 cgaattggta cgttagaact tcatgttcca cgaaccagag acggtaagtt tagtcctact
      361 atctttgaaa gatatcagcg aagcgaaaaa gctttgatcg ctgccatgat tgaaatggtc
      421 atttctggtg tctccactcg caaagtaact aaaacagttg aactgctaac ggatggtgcg
      481 actgtttcta aatcattcgt ttcaaatctg atgaaacagt tggatccatt tgtttttgag
      541 tggagaaacc ggagcctaga aggctcggaa tatccatttt tcatgtgtga tgccctgtat
      601 atgaaagtaa gggaaaatca tcgtattgtc tcaaaaggtg tatatattgg tatcggcatt
      661 gattctgatg gacgacgtac gattcttggt tttgatgttc aagacggcga atcggaagat
      721 aattgggata ccgtttttca atcgttcgtt caacgaggtt tgttcggtgt aaaactggtc
      781 atttctgatg cacacaaagg gttagttaag gctgttcgca agaacttttt aggtgcgagc
      841 tggcaaagat gccaagccca ctttttgaga aatatttttg ataagctgcc aaagaaagtt
      901 tcttctgatg taaaagacga gttgaagagt atctttaaag cctctgaact tgaattgaca
      961 cgtgaaagaa aagagcactt cttggaaaaa tatggttgtg attcaaaatt aagtgctgct
     1021 tgcgacattt tagaaaacgg ctttgaagat gctattcaaa ttttgtcttt tcctgaaaat
     1081 atccgccgaa ggattcgtac aaccaatgtg ttggaacggt tgaacgaaga gattcgccgg
     1141 agagaacgag tgattcggat ttttccaaat atcaattcca tcactcggat aattggaaca
     1201 cttttgatgg aaaaagacac tgagtggctg gcttctccac gaaaatattt agaatttaat
     1261 tcgaataaca tttagcacca tgaacctgtg cgagtctctt ctttctgagg agagacatca
     1321 agagtcgctg cgctactgct cctgacctct ctcctcagaa agaaaggcat ggcagttagg
     1381 ttcacaacgc aaaacattgt gcttttgatg aggttgaact tttacacaag attgtggact
     1441 tgactgtctc attaaaaagc ataaccatta ctccgatctg tttttaaaat tagtttctac
     1501 ctctaatata tatcgagttt ttattttgcc ttcagagaat actgtttctt tttttgcagt
     1561 tacaggttgt ttattttcgg aaaaagctaa tatagctaag attgaaacat ccatctggaa
     1621 tttatctttt ttactgcttt gatcataaaa gtctgcatat tcatcactga tattttttct
     1681 aataaattct tccatcataa aaatcacctc aaaacgatta tacgaactta cgttcttgtt
     1741 gtaaagcgat atttgagggg caaaaaaggg gcataatttg actgatatta agttagctat
     1801 tgatatatga atgacataca aaaatataaa atcttttaaa aatgcttata tatcaatgtt
     1861 tttgccattc ttttatatat ctgttatatg tcaaaaaaat gtgttcaatt tcaatttgta
     1921 tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaatcaa
     1981 aaacagcttc tcatgttttt gatttggttg gacttattac cgcagatata agcttttgag
     2041 caccgtgaat aaagaggtta tgaaatcaac gctctaacct gagtattaga gaaaaaaacg
     2101 caaaacatat catttatgtt ttgcgttttt tatttatctt tttttctcaa tacaatataa
     2161 aataggcggc tggttctttt ggttgataaa ccgataattc agcacattat atgtttcctg
     2221 tggcagtttg gaacaaaaag aatcaaccat atccagttct ttttctccac cttcatgacc
     2281 atagtagacg acaaggatca atcgtccgcg aggcacaagt cgtttcaata tttcttccat
     2341 agctgtgcgt gtagtctctg gcatagtgat gattgcttta tcactttttg ggagataccc
     2401 tagattgaaa atccctgctt tgagattttg ttgttcagct atcacttctc ctaacgtttc
     2461 gtggccttgt agaaacaatg agacacgttc ttctaactga cgctgttcta gcctttcacg
     2521 agtagcatcg attgcttgtt gctggatatc aaaactatag acatgaccag tttttccgat
     2581 gtgttctgct agaaaaaccg tatcgtggcc attgcccatc gttgcatcta cgacgtgatc
     2641 ccctggttgc aagatttctt gcagtaatgt atggctgaaa tgcaaagcag tttgcaacat
     2701 cagacgctca cctttttccg tacatcatat tttccttgga agctatctct gcgaagaagt
     2761 tcttcatcaa ttgcattcaa gacttcccat tttttcaagc tccacattgg tccaatcaag
     2821 gaatcccacg gcgcatcccc tgtcaaacga tggatgatga tctcttttgg tatcatttcc
     2881 agctggtcgc aaatgacatt gacatagtca ggacggctca ttaattgaag gcgaccttca
     2941 tgatagtccc gcaacattct cgttttgcgc atcagatgca tcaaatggag cttgattcct
     3001 tggatatctg aatctaaaat ggtgcgtctg acattttctt tcatcatttc taaactttcg
     3061 cctggtaatc cgttgatcaa atgcgtacaa acacggatat tatgtttgcg taatttagcc
     3121 acgccatcta aataagtttg ataatcatgt gctcggttga tcagttttga agtttcttca
     3181 aaagttgttt gcaatccaag ttctacccat aaatacatcc gctcgttcag ttcagcaagg
     3241 taatcaacta cttcatctgg cagacaatcc ggtcgtgtac cgactgataa tcctacgaca
     3301 cctggtaaat tgactacttg ctcaaatcgt tctttgatta tttcaactgg cgcatgggtg
     3361 tttgtaaaat tttggaaata aacgatgtat tgttggacat gaggccattt ttgatgcatc
     3421 atgtcgattt ctttctggaa ttggattgga agagggtctt caggtgcaac gatcatgtct
     3481 cccgaaccag atacactgca aaaagtacaa ccgccatggg caacagtccc atctcgattc
     3541 ggacagtcaa atcctccatc gatcggcacc ttgaaaattt tcccgccaaa ctcatttcgt
     3601 aaagcgtaat tccaagaatg atatctttta tttccttctg aataaggaaa agtcattaaa
     3661 agtccttctt tctgtaagag ttatctattt gttttctttt ttctctatac actggataag
     3721 tcaggcacag ccaagaaaga ctgagtaagt agccgccgat gatatcagta ggaaaatgaa
     3781 cgcccagata aatgcggctt atcccgacac ttactataat aaacatcatc agcaattgaa
     3841 gcaatgtgcg taaggttctt ttttgaacaa aagctggaag cagaaatagt aaagaaccgt
     3901 aaagaatcat actgcctgct gcatgtccac tagggaaact gtaacttgtt tcaactacta
     3961 agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt gatcaccggg ttgatgattc
     4021 cagcaattac aataaatcca gaaacgaacc aaaatgtttc tgcccttttt ttcttatacc
     4081 acatccataa agccgcacaa gcaaataaaa tgatgacggt aagaggatta ccgaatttag
     4141 tcataaagag ttggtagctg ttcagatcag gataagaagc tctgacaatg cctgtaaacc
     4201 actgatcgaa accagaaagc cacgacagat ggaactttac gatccagccc aaaatgaaaa
     4261 acaggacaag aaagaagcta ccagccacct gaaatgatgt tttatatttc atacaattcc
     4321 tcctttgaag caatgactta ttatagcatt tttggtccag cgaaaaaaac ggtaaagaga
     4381 taaaaccaat gtatgattcc tctttgaata accaatattc ttataaaaaa aataactgta
     4441 atcggaaaga cattttttgg taatgcaaat gaaatgttag ggaaataaaa tcagtgtttt
     4501 tcgaaatcct tttaaacaag aacgtagacg taggaagaac ttgaaaatga acagaagttg
     4561 ttcattacgg taaattacaa tatgattaca aaaaacaaca aagctcttaa ataactttta
     4621 ataaatatta agagaagtta taattattaa taatagataa attgaagttg gaggatagaa
     4681 gatggaaaac attgcaagaa aagaacggag acgtttagaa cagaccaaac gttttcgaaa
     4741 agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta ggctgttctg tcgctgcccc
     4801 attagtacag ccggttcctg ttcaagcgga tgaaacacct gttcagttcg gcgcaagaat
     4861 caatacagcg gcatttatca cagaaattgc tacttatgct caaccaatcg ctcaagccaa
     4921 cgatctatat gcatcagtga tgatcgcaca agcagttgta gaaagcggtt ggggcagtag
     4981 tgcgttatct caagcacctt attataatct ttttgggata aaaggcagct atcaaggaca
     5041 aaccgtctac atggatacgc ttgaattttt aaataatcaa tgggtaaata aaaaggagcc
     5101 tttccgacaa tatccttctt ttgctgaatc tttcaatgac aatgcctatg tacttagaaa
     5161 tacttcattt ggcaacggtt attactatgc cggtacttgg aagagcaaca cgaaatccta
     5221 tacagatgcg acagcttgtt tgacagggcg ttatgcgact gatcctggtt acgcaggcaa
     5281 attaaataat atcatcgcaa cttacggatt gacccaatat gatacaccag ctagcggaaa
     5341 cgcaggcggt ggagcgacta caggtggcaa taatactgga agtgcaggta gtactggcgg
     5401 ttcaagcaat tctggtaata caaactcagg ttcagcaact tcgaccactt atactgtcca
     5461 atctggtgat tctgtttggg gaatctcaaa taaattcggt atcacaatgg atcaattgat
     5521 ccaatggaac aacatccaaa acaatttcat ttatccagga caaaaactga caatcaaagg
     5581 tagccaggaa aatggctctt cgacgaataa ttccggtaac aataccaact cttcaggaaa
     5641 tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa tacactgttc aatctggtga
     5701 ttctgtttgg aagatctcaa atgatcacgg aattacgatg gctcaattga ttgaatggaa
     5761 caatatcaag aacaatttcg tttatccagg acaacagcta atcgtatcaa atggcggttc
     5821 ttctaatgct ggagcaaata ctggctcaaa tactgggaat acaagtggct ctagttcgtc
     5881 cagctcaagt tcttctaata cagcttcagg aacgaaatat acagttaaag ccggagactc
     5941 tgtttggagc gtctcaaata aatacggaat tacgatggct cagctaatcc aatggaacaa
     6001 tatccaaaat aactttatct acccaggaca acaactgact gtttcaaaca gtggatcaac
     6061 aaacagcacg tcaggtaata caggttcgtc aaccaataca aataacagca gttcagttaa
     6121 tactggcagc agttcgtctc aaggcactta taccgtgaaa gcaggtgaat ctgtttggag
     6181 cgtggcaaat aaaaacggca tcacgatgga tcagttaatc gaatggaata atatcaaaaa
     6241 caacttcatt tatccaggac agcaattgat cgttaaaggt ggaacttcgg caaatacaaa
     6301 tacaggttca actacttctg cagcgaaacc aaatacaccg aatacgtctg ctacgacaag
     6361 tacatcatcc actggtaata cgatgtatac cgtgaaagcg ggcgagtcag tttggagcgt
     6421 agcgaacaaa catcatatca cgatggatca gttaatcgaa tggaataata tcaaaaataa
     6481 tttcatctat ccaggacagc aagtgattgt gaagaaaggt tcatctcaat caacaaacca
     6541 acaatcagtt actggttcga aaacctatac tgtgaaatct ggagaatctg tttggagtgt
     6601 tgctgacagt catggaatta caatggctca actaatcgaa tggaacaata ttaagaacaa
     6661 ctttatttat ccaggtcaaa cgttgattgt agctaaataa aatagctgtt cgaagaaata
     6721 acgattgaat tccacaaaaa tatttgttac tataaggcta cttaaatatg acaacacttg
     6781 aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca tggctggtga gaatgtgcac
     6841 ggatgctttt gaactcgcct ttgagttgcc cttgctgaat acccgagtaa gcaagcggcg
     6901 gtgacggccg ttacaacgtt cgaggccttt tcactaaggt aaaattaggt ggtaccgcgt
     6961 agattacgtc ctataaaaag agcaatcttt ttataggatt tttttatttc ttttgtgaag
     7021 tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat tctgccggtt ttttcgttat
     7081 atttataata gaaacaagtg ttttcaatag aacaggagga cagcaaatga gttacaatca
     7141 taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa acgaatactt tcaacaccca
     7201 tgatgatcct gaaaagccaa aattttacgc actggatatg tttccctatc catccggtca
     7261 agggctacat gtagggcatc ctgaaggtta tacggcaacg gatatccttt cacgtgtgaa
     7321 aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg gatgcattcg gtctgcctgc
     7381 tgaacaatat gcgctagata caggaaacga tccagctgag tttacccaaa aaaatatcga
     7441 aacattccga cgccaaatca attctttagg atttagttat gactggaatc gcgaagtgaa
     7501 tacaactgat ccggagtatt ataaatggac acaatggatc tttacgaagc tttatgaaaa
     7561 aggtctagct tatgaagcag aagtagcggt aaactgggta cctgaattag gaacagttat
     7621 ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt ggctatgatg tagtcagaaa
     7681 accaatgcgc caatggatgc tgaaaatcac tgcttatgct gatcgcttat tggatgatct
     7741 agaattagtt gattggccag aaagcattaa agaaatgcaa agaaattgga tcggtcgttc
     7801 agtaggagca aatattgaat ttaaagtggc tggtacggat aaatcctata ccgtatttac
     7861 gacacgacca gatactctat tcggtgcaac ttactcggtt cttgcacctg aactagatct
     7921 tgttagagaa attacgaccc ctgaacaaaa agctgctgta gaagcttata tcgaagagac
     7981 agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa gaaaaaacag gagtatttac
     8041 tggtgcttat gcaatcaatc cagttaatgg caaggaaatc ccaatctgga tcgccgatta
     8101 tgtattatct tcatacggca caggagccat catggctgtt ccagcacatg atgaacgtga
     8161 ctttgaattc gctcaaactt tcggactaga aattcttcca gttatcgaag gtggagatgt
     8221 tgaaaaagct gcttacacag aagatggtac gcacatcaac tctgaatttc tagacggcat
     8281 gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta gaagaaaatg gcgtagggaa
     8341 aaaagaagtc agctatcgct tacgtgactg gctattctcc cgtcaacgtt actggggtga
     8401 accaatccca atcatccatt gggaggacgg aacagtcaca gctgtcccag aagaagagct
     8461 accattacgg ttgcctaaga caaaaaacat caagccaagc ggtactggag aatctccatt
     8521 agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta acaggtaaaa aaggccgtcg
     8581 cgaaacgaat acaatgccac aatgggcagg aagctcatgg tattacctgc gttatatcga
     8641 ccctcataac aaaaaagaac tagcagatta tgaaaagttg aagcgttggc taccagttga
     8701 catctacatt ggtggagcag aacatgcggt acttcattta ctatatgcac gattctggca
     8761 taaattctta tacgatatcg gtgttgttcc tacaaaagaa ccattccaaa aactatacaa
     8821 tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca aaatctcgtg gtaatgtagt
     8881 caatcctgat gatgtggtcg aaacttacgg tgcagataca ctgcgcatgt acgaaatgtt
     8941 catggggcca ttagatgctt cgattgcttg gagcgaaaat ggcttagaag gaagccgtaa
     9001 attcttggat cgtgtatggc gtttgatcgt tgatgagaac aataaaatgc gtgaccgtat
     9061 cactacattg aatgatggca agcttgataa agtgtatcat caaacagtga aaaaagtgac
     9121 agaagactat gaaaatcttc atttcaatac agcaatctcc caattgatgg tctttatcaa
     9181 tgaagcctat aaagtggacg ctctgcctta tgaatatatc gaaggatttg ttcaactact
     9241 cgcaccgatt gctcctcaca tgggtgaaga actatgggca atccttggaa atgatggtgg
     9301 tatttcttac gcaccatggc caacatacga tgaagcagca cttgttgaag acgaagtaga
     9361 agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc aatgttcccc gagacttagg
     9421 aaaagatgag ctagaaaaag cagcattagc caatgaaata gtgcaagaat atattgaagg
     9481 caaaacagtc agaaaagtta ttgcggtacc aaacaagtta gtcaatatcg ttgctaatta
     9541 attggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg gccagaatga
     9601 acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg atcaaaaagg
     9661 ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg gtcttgtaag
     9721 agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga tgtataaact
     9781 acctgaaatt gagagtacat accaaattca taaatttttt attagtggga ctagtgattg
     9841 tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat ggatcaagaa
     9901 ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga
     9961 gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt gctttttttc
    10021 ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc tgaagggatg
    10081 caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac aattgttgtg
    10141 cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg tcatttcgat
    10201 caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc tcaagatgat
    10261 gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca aaaaagtgat
    10321 tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga aagcaaatca
    10381 aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag atgggaaaaa
    10441 agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt cactggtttt
    10501 ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc aaaaatgtct
    10561 tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac gcatatacaa
    10621 aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac aacacaaaag
    10681 ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac agatagtggt
    10741 gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga atacaccctg
    10801 acaacggggc tgattcctga aggttattgg atggacaaca catatgatat ttccgccgat
    10861 ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc aagagataat
    10921 ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg ttatcgaaaa
    10981 gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg gatgtctgca
    11041 gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa aagtggaagt
    11101 acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt aggttttgtt
    11161 tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa agaatcagtt
    11221 ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc accaaatgaa
    11281 gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa aaaaggacca
    11341 atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg
    11401 caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga gattgtgagc
    11461 ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc atatttgttg
    11521 aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt tgaagtatag
    11581 cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt
    11641 attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga aactgaagga
    11701 gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc actagtacat
    11761 ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt agataatgtt
    11821 tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg tgattggagt
    11881 agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag gatcatgtct
    11941 gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca
    12001 gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga agttgcttca
    12061 tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa taattgttgc
    12121 tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc gtctggcagt
    12181 gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg
    12241 gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag ccaggtattt
    12301 tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt aatatcaaga
    12361 ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag attgactaaa
    12421 gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg ctttactgaa
    12481 aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac atttttgaaa
    12541 gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg aatttcatct
    12601 gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg gatcgagttg
    12661 atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg aagataagct
    12721 tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa taccgcactt
    12781 atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca gttcataaat
    12841 tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc agcttctgca
    12901 tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc ttccattcga
    12961 ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt tttgttcgca
    13021 atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa agtaagcttg
    13081 ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga agtaaaaaat
    13141 tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt tgtccgacta
    13201 atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt gaattttttt
    13261 gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat tgtccgttcc
    13321 tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa gacagggaac
    13381 aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt tcttatttct
    13441 gataagagat catcgatccc gattcccatg atcaagggca tggctacgac taataggttc
    13501 ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt gatcattttc
    13561 caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt atcccttctt
    13621 tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac agtagttctt
    13681 catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta tctgcttgtt
    13741 ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg tgtttatgaa
    13801 gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta tcatcgaata
    13861 catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt ttctggcccc
    13921 ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga agggtgtcca
    13981 ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt gaggcatatt
    14041 catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc ttctgaggga
    14101 cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga ataccaccaa
    14161 gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc aaactacttt
    14221 ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa
    14281 tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt tcaaaagcag
    14341 ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct tcctcaattt
    14401 cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat ttagggataa
    14461 tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag tattcgacta
    14521 cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg ccaaaccaca
    14581 aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc caagcaaata
    14641 ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc gttcgctttt
    14701 cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag aattctctca
    14761 atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt gcccctcgtt
    14821 tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta acccatgtta
    14881 gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc ggtgctggta
    14941 agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca ttcgtcattc
    15001 ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc tgttggaatt
    15061 tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat gctgaaaagt
    15121 aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt tcaactccaa
    15181 ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc tttgctacta
    15241 attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag gtaaggagag
    15301 cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc ctcctttttt
    15361 cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa aaaagaataa
    15421 actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag tatcggaact
    15481 atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa tccgttcaag
    15541 tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta tgctttatga
    15601 tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt tatatggaaa
    15661 aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc ataaagaact
    15721 ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt tgaaattgat
    15781 gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact gtatgatctc
    15841 aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt caagctttgg
    15901 gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta tcgaccctag
    15961 tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg
    16021 tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt tagaaatcag
    16081 agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt atctattgac
    16141 gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag aaatgaaaaa
    16201 ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta taaagaaaaa
    16261 ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata gatagaattt
    16321 tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt agcgatatat
    16381 acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt gttatgtata
    16441 attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag tgagattgta
    16501 tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa atacattgtt
    16561 gcttacatta ttacaatatg ttaataaggc aaaaataaat agatggattg aataaacata
    16621 ttttttaatc aaatagtccc tactgtgact ttcctcagct atcggtgata ctaccgatag
    16681 catttttttg cttataaaat aattataagt atttagagat ttttcttcta acattcttca
    16741 aacaagaaaa atcacaaagg ttattgttat ttttatatat tctttttaag aatgattgtt
    16801 tttttataga aattttaaaa aataaaacac gttattgatg gatagttagt ttgttatttg
    16861 gtaatattat agaagttgac tggtagtgat ttatttttcg tcgttttata aaaatgttat
    16921 tttttggaaa aattcacaaa tatgtaacga atgtttttct ggaatttgct gtgttaaaaa
    16981 gcagtatgac taatataaat actattgtca tttatgcaat tttttatgga tagtaatttt
    17041 agaggggaat caatgaagga agaatagaag ggaaaaatag ttgcaaatac gccagttagc
    17101 aaaaatagca atacatcttc aagctttaaa attcgagaga atcatcttgg tagtggaatc
    17161 aactacaaca aaaggattaa accaattaat agatagaatt accaatggaa aaatagaaaa
    17221 ggtagttatt ctatacaaaa gttgtttaat tcgcttcggt tatgaactta gtgaaaccct
    17281 ttgcaatata tggtacagaa tatagaaatt attgacatta cagaaaatga tgaagattta
    17341 atacagattg tcactatttg tagatacctt ttacaaggaa accgagccaa taaagctaag
    17401 aagatgatta aggagttgat ggaagacgat acaagttaag aaagtgaaac tacgcccaaa
    17461 taaagagcaa aaagagtaat tggacgtgga aaatgcaaga aatgaaccat cgattcggtg
    17521 gacagtttat tagtaatgac ggtttgagaa catccatgac gagaatgaag gaacatccta
    17581 aatgcgttta gttgaaccaa gtgccaaata atatggccaa ataagcgatc agttatcgat
    17641 gaatcccaag tattctaaac tatatattca ttttgatgct aagcattgat atattttttt
    17701 gggattgatg aaggttaacc aatacaggga ctcactgatg aaattctaga gtctgacctt
    17761 ggtatcaacg atttagtgat tgtttctaat ggaaaagtat aaaagtatat aagtacatta
    17821 acaaaataac tattagggtt acgctccagc ttgtgcaagg cactggcagg aggaatcgtg
    17881 aaatcataat tatggttaag ccacacggac aactaaaagt catacaaagc tatcgaatca
    17941 atcaacagaa gacaatcggc aatcattagc tttgtttaca gcttacattg caacgcttga
    18001 gttgaaccga aaatacgagg gaggtaacgc agccattttg agttggggaa tctttttaga
    18061 aaattatcgg gtaccttgtt gtttagtcaa aattataagg gggagtaata gtgaggaaca
    18121 aatttcataa actgttaagt atgtttctag ttatgcttat tgtgatcaat caatttggga
    18181 acataacggt tctagctgag tctttagcag aacagccaga gcgttcgctg acgatcgaag
    18241 ttgatcaaga ggaggctgct ttgggtgagc agttccagtt gcgagtcaga ggaacaccag
    18301 acctgctaaa tgcattggtc attgataaga gtgaggattt gattgaaggg gaagtgaagc
    18361 aactcaatga ttacgaacgt ctgattactt attcttccca gcattcaggt aaatacgaat
    18421 tgaccgcaag aagcggtgat attcaatcaa atacggtctc aatagaaatc agtgtaccag
    18481 aagaaagcac aagtgagtct tttaatgtac cagaagaaag cacaagtgaa tcttttaata
    18541 taccagaaga aagcttaagt gaatctttta atgaagccag atcatctgag ccatctataa
    18601 cgcaggaatc tcagattgaa agacaagagg agctcttgaa ttatgacatc ggcatctctg
    18661 ctgagcgccc cactttgccg agcaggcata cacaaacgtt aaagggatac attcaaggag
    18721 gcgctggcga tattcttgaa gaatatgggg agatggtcgt acgagttcct agagcatcga
    18781 ttggcttaat tgagttcatt agagtggagg tcgctgatga ttcaccttta aagccactgg
    18841 cattttacca agaaggcaca gacgttgttt ttccatttac ctttgattat gaaggattaa
    18901 atgaagccgc tcagtcgtat acattcagta tcacgtttga agttgctttc tttcaatcag
    18961 gcggtactga gccaaataat gactttcaag tgagtcttac ttatgctgag cgagcagcaa
    19021 acacggaatt ttcaattacg cctattcctt catcaagacc cattttttcg aaattatact
    19081 atggctctag agaaatcgac agccaaggaa atcttgggaa tctgctgatt gacgggcaag
    19141 acgtgaccaa cagtaataaa tttattttgc aggtaaatta tgaaggatca cgaaaagacg
    19201 gtgtagtggt taccgatgta ctaccgcaag gaacaacttt ggcaccgaat ccgactacat
    19261 ggagttccac tggaaataga gaacctattt caggtggggt tcggatcgca gaagtaacga
    19321 atccaggtga agcaaacgtt agaactcatt atgtttccga ggaatttgtg gacaaagtat
    19381 cttttaataa ggaaacgaat acacttgttg tggactttgg taatctagca gagggtcaat
    19441 cgtattttat tgaatatgct cttgaagtga ccgatgtcga cttgttcaat gaaggaagac
    19501 aagaggatgg gttttctcgg aatcgagcga gtatgaccta caataatcag gtaatgagcc
    19561 gagactaccg cttgagaatc cctgaaagta atcttccggc aatctcttta agaaaaagtg
    19621 tggacaaaaa tgttatcaat ctcaatgaaa atgaactaac gtatactctg catgtctcga
    19681 tgttttctgg aacaagaaat acgcttgtta ttgaagatgt tttggaagaa ggcatggaat
    19741 ttgtgtcatt tctatcagat attccctctt tcattgatgc cgaagtcaat aatggaatag
    19801 ttcgtttcag tgcgaataat acgattggtg gtccgacaag ttttaccctc tcttttaagg
    19861 tcaatgtaga ggcgtatgcg gtcggcgatc aaattttaaa ctttgcaacg cttgtgaata
    19921 gtgacaatcg attgaatacg agtactgtaa gaacaaggaa aatcgatggg cgtattaaag
    19981 taattaaaac tgatgagaat ggccaaaggc tggcaggagc aaaatttgaa attttatcaa
    20041 gcgattcaca agtgatacaa gaaggtataa cggcagaaaa cggagaattt ttaagtgaac
    20101 cgcttcctgt aggtagctat caagtccgag aaacagaagc accggaaggg tatcttttag
    20161 atgcgacggt acatgatgtt tttataaatg aatactccat agaagaagtg acgttaacca
    20221 ttgaaaacat ccttgatacg ggtggtgttg aactaattaa acatgctgaa ggcagtggtg
    20281 aagtccttca aggagctgtt tttgagttac agaaccgaga aggagaaacg ctacaaaccg
    20341 gt
//
LOCUS       DACIRY010000045        19304 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.046, whole genome shotgun sequence.
ACCESSION   DACIRY010000045 DACIRY010000000
VERSION     DACIRY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.046"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            238..1629
                     /locus_tag="IYE22_001390"
     CDS             238..1629
                     /locus_tag="IYE22_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ9506607.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1688..2491
                     /locus_tag="IYE22_001391"
     CDS             1688..2491
                     /locus_tag="IYE22_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9506608.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2524..3378
                     /locus_tag="IYE22_001392"
     CDS             2524..3378
                     /locus_tag="IYE22_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAQ9506609.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3375..4076
                     /locus_tag="IYE22_001393"
     CDS             3375..4076
                     /locus_tag="IYE22_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAQ9506610.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4084..5151
                     /locus_tag="IYE22_001394"
     CDS             4084..5151
                     /locus_tag="IYE22_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ9506611.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5144..6109
                     /locus_tag="IYE22_001395"
     CDS             5144..6109
                     /locus_tag="IYE22_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9506612.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHQENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6154..7128
                     /locus_tag="IYE22_001396"
     CDS             6154..7128
                     /locus_tag="IYE22_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9506613.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7199..8686
                     /locus_tag="IYE22_001397"
     CDS             7199..8686
                     /locus_tag="IYE22_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAQ9506614.1"
                     /translation="MFIYILRIVIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAE
                     LGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKN
                     STDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIA
                     LYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIG
                     NMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGN
                     FAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILI
                     VLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIG
                     TIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGG
                     STFLQFITKTLITIVTTSILYLLLYFRREEFTFIKKILFRYILKK"
     gene            8743..9858
                     /locus_tag="IYE22_001398"
     CDS             8743..9858
                     /locus_tag="IYE22_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ9506615.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            9855..11270
                     /locus_tag="IYE22_001399"
     CDS             9855..11270
                     /locus_tag="IYE22_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ9506616.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            11303..12250
                     /locus_tag="IYE22_001400"
     CDS             11303..12250
                     /locus_tag="IYE22_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ9506617.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(12290..13450)
                     /locus_tag="IYE22_001401"
     CDS             complement(12290..13450)
                     /locus_tag="IYE22_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ9506618.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            13615..14790
                     /gene="mltG"
                     /locus_tag="IYE22_001402"
     CDS             13615..14790
                     /gene="mltG"
                     /locus_tag="IYE22_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ9506619.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            14926..15405
                     /gene="greA"
                     /locus_tag="IYE22_001403"
     CDS             14926..15405
                     /gene="greA"
                     /locus_tag="IYE22_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ9506620.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            15573..16304
                     /locus_tag="IYE22_001404"
     CDS             15573..16304
                     /locus_tag="IYE22_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ9506621.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            16301..17368
                     /locus_tag="IYE22_001405"
     CDS             16301..17368
                     /locus_tag="IYE22_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ9506622.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            17375..18007
                     /locus_tag="IYE22_001406"
     CDS             17375..18007
                     /locus_tag="IYE22_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9506623.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            18147..18812
                     /locus_tag="IYE22_001407"
     CDS             18147..18812
                     /locus_tag="IYE22_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ9506624.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            18840..19133
                     /locus_tag="IYE22_001408"
     CDS             18840..19133
                     /locus_tag="IYE22_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ9506625.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(19201..>19304)
                     /locus_tag="IYE22_001409"
     CDS             complement(19201..>19304)
                     /locus_tag="IYE22_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ9506626.1"
                     /translation="FQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         6740 a         2703 c         3713 g         6148 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
     1561 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
     1621 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
     1681 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
     1741 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
     1801 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1921 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1981 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     2041 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     2101 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     2161 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     2221 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     2281 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     2341 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     2401 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     2461 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     2521 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     2581 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     2641 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     2701 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     2761 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     2821 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2881 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2941 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     3001 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     3061 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     3121 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     3181 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     3241 gtttttggtg actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca
     3301 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     3361 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     3421 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     3481 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     3541 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     3601 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     3661 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     3721 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     3781 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3841 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3901 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3961 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     4021 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     4081 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     4141 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     4201 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     4261 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     4321 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     4381 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     4441 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     4501 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     4561 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     4621 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     4681 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     4741 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     4801 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4861 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4921 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4981 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     5041 gtacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     5101 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     5161 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     5221 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     5281 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc atcaagaaaa
     5341 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     5401 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     5461 gaaaattcat gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata
     5521 taagaaagct aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc
     5581 tgttttagaa ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact
     5641 ttttaaagat aatcgttact tggatggaaa aacatttgag gatgcatata taataataga
     5701 tctattattt caaacagaaa aaattgttgt ttccaatgaa gagaaatatt tctatcttca
     5761 aagaaatgat agtattatgg gaaaaagttt ttctatgaat aatttcgatg ttatagaagc
     5821 atggcaatat aacaaagaga agattttaga tgcttttcca gatttgcatg attcctatta
     5881 tcgccgacta tgttgggcat atttttacgt gttggataaa atggttttat caagtagtta
     5941 tcaacaaatt ccacgtaaaa aaacagtaat tgaattttta aagaagaaca gaaattttat
     6001 attaacttat aagggattca ctaaatcgcg caaaatagct attattgctc tgagctttag
     6061 agtagattgg tataggaaat tggttgaaca aaaaaataaa aaaaattaga atgtttaaat
     6121 tagtaaataa aagaaaagga attgaaaact aaaatgtgca gaataagtat aatagttcca
     6181 gtttataatg ttgaaaaata tttaaaaaag tgtgtcgatt caattctagg ccaaacattt
     6241 accgattttg aattattatt aattgatgat ggttctacag ataattcagg aagtatatgc
     6301 gatgaacttg cgaaaacaga caaccgaata aaagtgatac ataaggaaaa tgggggtcta
     6361 agtgatgcca gaaatattgg tattgaggta gcaaagggag attttattgg gtttatagat
     6421 agtgatgatt acattgatga agatatgtat gcatttttat ataacaatat attgaaatat
     6481 gatgcagagt tatcaatgtg tggtatttat gacgtccata agaataaaga aattaaaaag
     6541 ttaactcctt tttctcaatt ggttactaaa tctgaagcaa ttgaattagt attagatggt
     6601 aagctagttg ttgctaatgc agtaagcaag ctatataaaa aagaattatt tgagaatgtg
     6661 agatatccaa aaggaaaaat tgctgaagac gcagcagtaa tactgaaaat tatcaatcag
     6721 tgtaataaaa tccatgttga tacttctcaa aaatattatt attatcatcg tggagatagt
     6781 attactagta aaaaatttac aaaaaaagat tttgatataa ttgatgtatg gaatgagaat
     6841 gaaaaatgga ttattaaaaa ttatcctcaa atgtacgata aagtacatac tagagtttgt
     6901 tgggcttact ttgttgtgtt agataaatta gttttaactg aagaaaaagg actgtatcaa
     6961 gaaaaaaaac aagtaagaga gtatttaatt gataatttct tttttattat aaaaaataaa
     7021 cgattaactt ttcaaagaaa aatatctatg ttattgctta cttttggaat tgctgcgtat
     7081 aaaataccgg ttaaatatca gtataagaaa gtaaaggctg ttaattaatg ctttagttag
     7141 gatgaattag atgaaaaaaa gcaggttaga gtattcttta ataaattctt ctatatcaat
     7201 gtttatatat attttaagga tagtaataca atttgtaggt agaagttttt ttatttattt
     7261 cttaggagcg aaatatttag gattaaatgg tttatttaca aatatactta gtttcttgtc
     7321 gttggctgaa ctaggcatag gctcttctat tatttattcc ctatacaaac cattagccca
     7381 aaaggatgaa agtaaagtaa aggctttaat gaatttgtat aagaagacat atgaatttat
     7441 tggtattttt atcggcatag taggagtatt gattattccg ctgttacctc tatttattaa
     7501 aaatagcaca gacataccac atatgtatcg atattatgta ttatttttat taaattcagt
     7561 tcttagttat tttttcacgt ataaaagatc acttgcaata gcagatcaaa aaaattatct
     7621 ggttgcgatt aatgattttg cctttttatt ctttatgaac attgtacaag taattgcttt
     7681 gtacttttat tctaatttta ctctatttct aatagtgcag attttgttta ctctattttc
     7741 taatataaat atttccagaa cagttgacaa gagatattca tatttaaaag agaaagatgt
     7801 agtttctcta gataagcaaa caaaagatga agtaaagaaa aatgtcatag gtaatatgtc
     7861 ctcaaaaata ggcggggtta ttgtaatggg gacagataat atattaattt ctacatttgt
     7921 taatttaaca gctgtaggaa tttattctaa ctattcattg attgtcacaa gtgtgcaaaa
     7981 tttatgtaag caaattacta attctatcac ggcaagtgta ggaaattttg ctgtatcaaa
     8041 taaaaaaaag gattcatttg agctatttaa aaaacatttt tttatcaacc actcactaat
     8101 ctttttttct tctatattgt tattgacttt aatgaatccc ttcattagct tgtgggtagg
     8161 agccaaatac atgctaccta tgtcaacgac tattttaatt gttttaaatt atagtgtaca
     8221 ggtttataga aatacatcct tcgtttttat agagtcattt ggtttatatt ggtctcaacg
     8281 gaaaaagcct ataattgaag caggtgtaaa cttgttatta tctttgcttt tgttaatagt
     8341 ttttgatatg ggaatcaatg gagtacttat agggactatc ggatcgtctc taggatttgt
     8401 gatttggtat gaagcattta tcatatataa aacggtattt gagaaacgtt ttattcattt
     8461 ctttttaact ttctggcgaa gttttataga attaataatt tcggcaatag tcgtttttct
     8521 attaaatgaa tatttagcaa atggaggcag tacgttcctt caatttataa ctaaaacctt
     8581 aataactata gtaacaacat cgatattata tttgttatta tattttagaa gagaagaatt
     8641 tacatttatc aaaaaaattc tttttaggta tattttaaaa aaatagcttt ctataaagga
     8701 gtgttttatt tgaaaatagc tatactatct ccagctttct caatgggagg agctagtatc
     8761 gtggcctcaa aaattgcaaa aggattaata gatagtggcc atgaagtttt ttttgtttct
     8821 tacttagaaa gtgaaaaacc acaagtagaa tctaagtatt atgatttata cgaaaaaaaa
     8881 atttttttat ttaaaaattt ttctcgattt cataagatat tagagtatag attgtttgga
     8941 ggctttgttc ctagtagata tataaaaaaa gaaaaagaac aactaaaaaa agttctaaag
     9001 aaagaaaaac ctgatgtcat catattcaat acttttatac ctagtgtact attctccgaa
     9061 ttcctaaagt atgaatttcc aacagtgaaa ttaattactt ggatgcactc tgatcctgat
     9121 tacagcttaa atcatattgc taaattttat aaaaaagaat atagaaaaag ttttgaacgc
     9181 gtggattcca ttgtttgtct ttcgcaagat acaaaagaaa aactttcatt atacaataac
     9241 catattaaag ttatttataa cccattgaca ttaagcggct ctagtttaat aagtaacctt
     9301 gataaaaaaa ttatttcatt tactgccaga tacgatataa atattaaagg gtttgattat
     9361 ttatgtgaag tagctaaaaa cttacctgtt ggatgggcta taaggattgc tggagatggt
     9421 acagaagaag aacgtaaaga gctgaaaaga ttaattgaga aaaatggtgt gttagataaa
     9481 attatttgcg caggtgcatt atttggagat gaattaatca atcattatat agaaagttct
     9541 attttcatat ctacttctag aagtgaggga ctaccattag tgttgataga agcaatgact
     9601 tatggattac ctgtaataag ttttgatcat ggtggtgcta aggaaatatt acaagatgga
     9661 aattatggaa ttttaattaa gaattttgac gtacaagata tgaataatga gatagaaaaa
     9721 ttgtctaata atcgtttact tatggaaaaa tatcaaaaat tctctttaga aagagctaag
     9781 gattttaatg aaaaaaaaat aattggtgaa tggcaaacat tgttaaatga attaggaaat
     9841 gaggatgtta gaaattgagg acttcgatta aaaattttaa tttatggaaa gaatggtatc
     9901 tatatatatt ttttataggg atgattattc gtgcaataaa tttttatagt ttattaccgt
     9961 ccacttttga tagtattttt tttaaactta taggaatttc aggagttttg ctattaggga
    10021 tagatttcat aattaaattt aataaaagaa aagtaactta taacatttta ttgatagcat
    10081 atatggtgat tttagtaata acgagttttg ttcataaaga atatggtcta ggagaaaact
    10141 taaagactat aatgtgggca atcttacagt attttttgat ttatcagttt gcttttgaga
    10201 atagcgataa taaacgattt tttaaaagga taagttgggg atttatttat agttggtttg
    10261 ttttagtatc aatgtcaatg gttcttttct ttggtaaatt cggctatgag aaatattata
    10321 atgctagaca taggatcaga attggttttt tagaatcaag actgtttggg acgttttctg
    10381 atattaacaa tgctgctata gcatctttag ttgttattgt cctaatctta ttttatcttt
    10441 ataataaaaa agtgacagga ttaaataaaa tcattttttt aattaatctt ccattacaat
    10501 ttatttatat agtattaagt gggtcacgat ctacctatgt ggtaagtata ataatagttg
    10561 ccatatcatc ttttattgtt gtgttaaaac gaaatggaac aaaaaaagat ttaaaaaact
    10621 atatccttgc tatagtatct tctattggtt ctgttctgat tttattcgct ttgtttaatg
    10681 caactaagat actttttcaa gaattattac ctcatttaaa agaaataaat ttttttaatt
    10741 ttacagagcg tcataaagaa ttaatgaata atagtcaaca tattaataat actcaaacac
    10801 ctgataatac tcttgttagg aaagacgtag ctgaaaatac agatatttct aatgctagaa
    10861 ttactatatg gaaaagtgca tgggaaatat ttaaaacaag ttggcttatc ggagtctctc
    10921 cgaaaaatgt agtatcttat gcacagtcaa ttattccaga tggatatatt gcgaaagtag
    10981 gaattgccat tcataatgca tacttgaatg tattaacatc gacaggtatt ctaggaataa
    11041 ttcctttcat ggctttttta ataaagagtt ttgtgctgaa tgtttatcga atcatctata
    11101 ataaatgcat tggtaaagat tatcattttg tttactgttt agttatttta tcttatgcac
    11161 tatacgcagc tcttaacaat gaattagtct atgaaaacac tgtaggaacg tttgtttttt
    11221 ggctattttt aggaagagtt aatagcaaaa ataattctag ctatttttag taataaattt
    11281 tatcttgaaa agaggagaaa aaatgttgaa ttacttgatt accggtggcg ccggcttcat
    11341 tggctcgacg ctcgcgaatt atctaggaaa agaaaacaat gtagttgtga tcgatgattt
    11401 atcgatgggg aaaaaagaga atctggatga atcgaagcac atcacgttta ttgaagggga
    11461 tgtcgctgac aaaaatttga tggaaaaggt catgaaagaa taccatttcg attatatttt
    11521 ccatctagct gctgtagcta gtgtggcaga ttctgtagaa cgtcctctag aaactcatcg
    11581 tgtaaatttt gacagtgcgc ttttattgct tgaattggta cgaaaatatc aatctggttt
    11641 aaaacgattg gtatttagtt catcagctgc agtttatggc gatgaaccaa cgttgccaaa
    11701 aaaagaagaa tcggtcatcc gtcctttaac accatatgcc atcgataagt ttgcagcaga
    11761 acaatatgtt ttggattact gtcatttata tgatgttccg actagtgctg tccgattttt
    11821 taacgtttat ggtccaaatc aaaatcctaa ctctccatat agcggagtta tttcgattct
    11881 agtggaccga tacaaaaaac aattggcagg agaaaaaaca gaatttactt tatttggtga
    11941 tggtagccaa tctagagact tcgtttatat agaagatgtt atacaagcac ttttacttgt
    12001 tgctaaggaa gaaaaagctt taggacagca attcaatgtc ggtacaggaa aaagcactac
    12061 gctacttgaa ttgattcatt caattgatca aatcttagga acagagctag cgcttaaata
    12121 tgaagcggag cgttcagggg atattcgtga ttctttagcg gatatttcaa agatccgttc
    12181 tttaggttat caacccaaat ttgatatatt gaacggaatg gaacgttatt taaagacaga
    12241 aatcgattga aacaaaaaac cagcatcctg ctggtttttt tgtctcgact tagcttttta
    12301 aattaacacc ttccatcagt tcgtaaatct gatttcttaa atcagtggcc ggcaatttga
    12361 tgtttttatc agattggata ttactttcca tgacgattgc atcttttgtg tcttttttca
    12421 tcgccgctac tgcatggaat cgattgatat ttccctgagc aaggatataa tcatttccac
    12481 tgtaaacacc gccgctgtaa ggatgtttat caccattatt ttgccacaat gagaaaagta
    12541 gttcttttgg tataatatcg ccagacagaa cactggtaaa gaatttgttc aagtcataaa
    12601 cagacatact tatatttcct gtgcctaatt catttcggat gtctgtttca ggctcgacca
    12661 atgcttttga ataatcgtca gttgcagaca tttggtaaga tacagcatgc tgaggggaat
    12721 tttttacatt ttcataaaaa tctgtatgtt ctagatggag tggccgaatg atttcttttt
    12781 ttaaaagagt ttcataaggc tgattcatta gtttgatcaa tacccctgta agcaatgtat
    12841 aatttaatgg ttcataatga tacgtatcat aaccagtaaa tttcaaatga tgaagggcaa
    12901 actgaatgat ctcactatct gacataggtt tttttggaga agcggttcga tgaagaccag
    12961 agcgcatgta aagcatattt ttgattgaga tttgctgact tccaggaatc tgaggataaa
    13021 attcagatag ctttgtgtca aaagttatct tgccagattg aatttgtttt aggataagta
    13081 aagcagtcat cgctttttgg atcgaaccga tttgatagta aagagaagga gaattcgatt
    13141 cttttatgcg tagatttgcg tacccatatc ctttttctaa tatcactcga ttatttttgc
    13201 gtacataaat cgaaccgata taatctgatt tttgcagtaa ttgatcaatt ttgctggcaa
    13261 gcatctgctc attagatgtt aaattggaag caagttgtgc gggttccgtt ttataaattt
    13321 gtggttgagg ttgttttgtt aattgccatc ctgtaatgat gcctgttgaa aagaagctga
    13381 cagccagcaa caaaactgcg agaaaagaaa gagagatctg ctgttttttt tgatgttttc
    13441 cttttgccat attaaatcct tcctatccat ttttaactat ttttcattgt aatcctcaaa
    13501 aagagcattt gccacttaca attttgacat aatatttggt aaaataattc acggcagtag
    13561 aaatttcatg tataataaac agagttacga agatgatagt aaggaggagt tgttttgtca
    13621 ggaaacaaac aacacccaag caaagacaaa aaagaagcca gtaaggcagc tacagcagat
    13681 ataatcgaca agcggagaaa tctgcgtaaa aaagaagata agatcgtgag gaagatcatt
    13741 ttagtgatcg ctcttacatt attgattatt ggcggatttc tcgggtttac cgtatatcgt
    13801 tatgtagata gcgggttgaa gcctttagat aaatcggacg accaattagt ccaagtggaa
    13861 atcccaagcg gctcttctaa taagcagatt ggcgaaattt tggagaaaga caatattatt
    13921 aaaagtggga ttgttttcaa ttattataca aaatttaaaa acttaactgg ctttcaagct
    13981 ggttattatc agttagcgcc taatatgact cttgatgaga ttggcaagca gttgcaagaa
    14041 ggcggcacat ctgagccaac aaaagttgca gatgggaaaa ttgccattcc agaaggctac
    14101 gacatcgatc agattgctga gcgggtagcg aaggtaactg gtaaagacaa gaaagaattt
    14161 ttagatttag taaatgatga gactttcttt aacgagttgt atcaaaaatt cccagaattg
    14221 ttggaaagtg cctctaaagc tgagaacaca aaataccgtt tggaaggcta cttattccca
    14281 gctacttatg attactacac gagtacttcg cttaaggatc ttgtgattga gatggtgaac
    14341 aaaacaaata cggtcatgca aaactattat tcagctatca aacagaaaaa tttgactgta
    14401 caagaagttt taactttggc ttctctagtt gaaaaagaag gcgtaaaaga aaatgatcgt
    14461 aaaaatattg cacaagtctt ctttaaccga atcaaggcaa atatgccact tcagtcagat
    14521 atctctgttt tatatgcatt aggagaacat aaagaattgg tgacgtatga agatacagct
    14581 gtagattcac catacaattt gtataccaat accggctatg ggcctggtcc ttttgacaat
    14641 ccaagcgaag aggcaatcaa ggcagtatta gaaccagcag aaaatgacta ttattatttc
    14701 gtagcagata cgagcactgg aaatgtttat ttcgctaaaa cgtatgaaga acatatggaa
    14761 ctagtgcaaa aatatgtaaa taattcttag aaaaacaggg gctagtcatt gatcattttt
    14821 agaaaaacat ttacatttta agaaaagcgt ggtaatcttt tttggactat attcctcaaa
    14881 agattacctt ttgcggtatt ttatcgaaaa aaggagacgt tacacatggt agagaaagtt
    14941 tatccaatga ctctagaagg taaagaaaaa ttagaaaaag aattagaaga actaaaaaca
    15001 gttaaacgcg gagaaatcat tgaacgaatc aaaatcgctc gcggtttcgg agatctttcc
    15061 gaaaactccg aatacgaatc agctaaagat gaacaagcat ttgttgaagg aagaatcaca
    15121 acaattgaaa atatgatccg ttttgcacaa atcatcgata atgacggcgt agatgcagat
    15181 gaggtatcga tcggtaaaac agttacgttc atcgagttgc ctgatggaga tgaagaagag
    15241 tacacgattg tcggaagtgc tgaagcagac ccgttttctg gaaagatttc caatgattct
    15301 cctatcgcgc aagcattgat tggcaaacgt aaaaatgatg aagtaaccat tgctacacca
    15361 ggtggcgata tgttagttaa aatcgtcaaa gtagaacaag cgtagtctga ctgaaagatc
    15421 tagagagttc atcttactga tgacgcactc tagatctttt ctctttgagt attgcaggtt
    15481 tcactgaacg atcttacatc taaagtccta tagccaacag tcaaaaaaat aggtatgatg
    15541 aaatgagaat gtgtcaggag ggagaaaaag cgatgaatag ttcttggcgt ttttttgtgg
    15601 tagtcgaagc actgctgctg ttatttgctg tttggcaaat cgtaaacaat actggacttt
    15661 tgcttttagt aatattcggt attttcaata tatatctagc tatgcgcaaa tatcctcgta
    15721 ctaaatttca aaattttcaa ttgatcttag ggagcttggt catttttttt agcttagtca
    15781 acagtcctgc tttatggatg atggttgtat tggccgtttt atttatcggg ctgaaaggtg
    15841 tagagatttc aggaatagat ttgacaaaaa atgctttttg gcgaaaaaaa cagatcatga
    15901 tggtccaaac agagcaatta aaaacacata ataatgagcg taaaagacag caattatttg
    15961 gtaaccaacg tattggaaac gacgtctatg aatgggatga tatcaacatt gccatcattt
    16021 ctggtgatac tatcatcgat ttgggcaata cacttttgcc aaaagatgat aatattgtga
    16081 ttgtaagaaa agggataggc agaacacgga tcttagtacc cctgggagtg gctattcgtt
    16141 tagagcatgc gacattagta ggaaatgtct tgtttgaaga agagcagttt tccttaaaaa
    16201 acgaacagat caaaatctgc agcagtgatt atgatgaaaa tcctcgcaga ttgaagatca
    16261 ttaccaatac tttggttgga gatatcgagg tgatacgaat atgatgggaa gaatatccaa
    16321 agcaatgcta gctgtttatt cggggattgc ggctttcctt attattctat tttcactttt
    16381 cacttatttt tatgccagta accaaagtca ttggtgggga gaattgctgc gtgcgcgttt
    16441 attatacgtt ccgcttatct tccatttgat ggcaatttca ttaggtgtag gattgatcgt
    16501 atttctattg ttatcactta ttcaaaaggc aaaatacggg aaaatcgaag aaaagcttcg
    16561 tgcactttct tctggcaatt atgaatccaa acttttgcat cagccgatcc caagcgcgtc
    16621 ggatgatctg tacatcaaag atattgatat ggaaattact aaaataaaag aaaaaatgat
    16681 cgaagtatcc agtgaattac agattatgac tagccgtcct caatatgtag atggacaaac
    16741 caaagaagag attttaaaat tagaaaggca tcgattagct cgtgagcttc atgattcagt
    16801 ttcacagcaa ttatttgcag cgatgatgat gatgtcggca ttgacggagc aagcagaaaa
    16861 aagtgagaca ccagaaatgt tccgtaagca gttgaaaatg gtggcagaga tcatcaacgc
    16921 ttcccagtct gagatgcgtg cgctgcttct tcatctgcgt ccagtcaatt tagaagaaaa
    16981 aagtttaaaa caaggcattg agcagttatt gaaggaattg cagaataaaa tccagatttc
    17041 gctaaaatgg gaggtagaag atgtaaaatt aactagttcc attgaggatc acttgttccg
    17101 aatcgttcaa gaattgttat caaacacatt aagacatgct aaggctaatg aattagaagt
    17161 ctatttgcat aaaatagata acaatctttt attacgtatc attgacgacg ggacaggatt
    17221 taatatgaat gaaaccaaaa cgggaagtta tggattgaac aatatcaaag aacgagtagc
    17281 tggtattggt ggaacagtaa aaatcattag ttttaaaggg caaggtacga gcgtggagac
    17341 caaggtccct ttgatgaagg aggcatagat aaagatgata aaagttttat tagtagatga
    17401 ccatgaaatg gtacgcttag gcgtctcttc ttatttatct atccaagaag acattgaagt
    17461 gattggagaa gctgaaaatg gacgtcaagg ctatgaaaag gcgatggcac ttcgaccgga
    17521 tgtcatttta atggatctag tcatggaaga aatggatggc atcgaatcaa caaaagcaat
    17581 cttaaaagat tggccaaaag ccaaaatcat tatcgtcaca agttttattg atgatgaaaa
    17641 agtttatcct gcaattgaag caggagcagc aggatatttg ttgaagacgt ctacagcaca
    17701 tgaaatagct gatgctataa gggctacgca acgcggcgag cgagtactag aacctgaagt
    17761 gacaacaaaa atgatggaaa aaatgagtcg gcgaaatgat ccagtattgc atgaagaatt
    17821 aacgaatcga gaaaatgaaa ttttgatgct gatttctgaa gggaaaagta atcaggaaat
    17881 cgcggatgaa ttgtttatca cactgaaaac agtcaaaacg catgtgtcga atatattagc
    17941 taagttagag gtagaagacc gaactcaagc agctatctat gcgtttaaac atggtttggt
    18001 gaaataaaaa cgatgataaa gaggataatc aaaagctgag aaaataacta ttatttttct
    18061 tggctttttt acaataatga tgcttgcttt tgtttttgga aaggctttgt tatactgaga
    18121 aaagaagata ctaaaggagg aaaatggtga aacaaaattt tgcaattgta ggccttggac
    18181 gatttggcgg aagcatctgc cgtacactga tcgaagctgg acaagaagta ctggctattg
    18241 acagtaatga agatcgagtc aatgaatata tgaatatagc cacccatgct gttgtggcaa
    18301 atgcacagga tgagatgaca cttcgttcat taggtataag aaatttcgat catgtaattg
    18361 tcgctattgg agaagatatc caagccagca tcttagttac tctgatggta aaggaaatgg
    18421 gcgtaccgaa tattttagca aaagcccaaa atgagtacca tgcccgtgta ctagagaaga
    18481 tcggagcaga tcgtgtcgta caccctgagc gagacatggg gatacggatt gcacataact
    18541 tagtatcaaa gaatattcta gactacttag agctgtcaga taaatattct ttagcagaaa
    18601 tccgagtctc caatcctaaa tttttcaaca aaacattagc tgaattaaat ttcaggcaac
    18661 gttttgactt aacagtagtc gccatacgaa gagtagatca aacggttatc gcttctcctg
    18721 cagcagatga atatgttcgt gaaaatgata atctactggt tattggagag acaggcgatg
    18781 tggatattct agacgataag atgaatcaat agaaagaaaa gagaaagtag gttgttggga
    18841 tgaaattaac ggtaacgcca aaagcacaaa agtggtttga ctcagaaatt gatctgccag
    18901 aaacttacgg aatacgtttt tttggaaaag tctacggcaa aacagaggtt catgatggtt
    18961 tttcgattgg aatgtcagtt gaacagccag aacatcctgt aaagaaagtg gaaatggatg
    19021 ggacactttt ttttatcgag gaagcagatg aatggttctt taaaggatat gatttacttg
    19081 ttgattatga tcccaacctg gaggagccaa cctatcactt taaaaaacaa taaaaaaaga
    19141 gagggtgaca caatttttgt caccttctct tttttctttt gaaaatattg ttttcagcta
    19201 ttaatatcct gcagacaatt ttacttgatt ggtttgaagc tcatgggtgg caaagtagct
    19261 tttcagattg gttaaaaaaa tagccattaa tttcttttga aaat
//
LOCUS       DACIRY010000046        18725 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.047, whole genome shotgun sequence.
ACCESSION   DACIRY010000046 DACIRY010000000
VERSION     DACIRY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.047"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            433..660
                     /locus_tag="IYE22_001410"
     CDS             433..660
                     /locus_tag="IYE22_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ9506627.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            783..1109
                     /locus_tag="IYE22_001411"
     CDS             783..1109
                     /locus_tag="IYE22_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ9506628.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1177..1890
                     /locus_tag="IYE22_001412"
     CDS             1177..1890
                     /locus_tag="IYE22_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ9506629.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2200..3051
                     /locus_tag="IYE22_001413"
     CDS             2200..3051
                     /locus_tag="IYE22_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9506630.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            3172..5037
                     /locus_tag="IYE22_001414"
     CDS             3172..5037
                     /locus_tag="IYE22_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9506631.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5051..6493
                     /locus_tag="IYE22_001415"
     CDS             5051..6493
                     /locus_tag="IYE22_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9506632.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            6601..7302
                     /locus_tag="IYE22_001416"
     CDS             6601..7302
                     /locus_tag="IYE22_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9506633.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7474..7953
                     /locus_tag="IYE22_001417"
                     /pseudo
     CDS             7474..7953
                     /locus_tag="IYE22_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            7964..8470
                     /locus_tag="IYE22_001418"
     CDS             7964..8470
                     /locus_tag="IYE22_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAQ9506634.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            8881..9456
                     /locus_tag="IYE22_001419"
     CDS             8881..9456
                     /locus_tag="IYE22_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ9506635.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            9456..9896
                     /locus_tag="IYE22_001420"
     CDS             9456..9896
                     /locus_tag="IYE22_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAQ9506636.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            9912..10613
                     /locus_tag="IYE22_001421"
     CDS             9912..10613
                     /locus_tag="IYE22_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ9506637.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(11111..11536)
                     /locus_tag="IYE22_001422"
     CDS             complement(11111..11536)
                     /locus_tag="IYE22_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506638.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            11767..13092
                     /locus_tag="IYE22_001423"
     CDS             11767..13092
                     /locus_tag="IYE22_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9506639.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            13094..15394
                     /locus_tag="IYE22_001424"
     CDS             13094..15394
                     /locus_tag="IYE22_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAQ9506640.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            15401..16372
                     /locus_tag="IYE22_001425"
     CDS             15401..16372
                     /locus_tag="IYE22_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9506641.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            16394..17791
                     /locus_tag="IYE22_001426"
     CDS             16394..17791
                     /locus_tag="IYE22_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9506642.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            17947..18570
                     /locus_tag="IYE22_001427"
     CDS             17947..18570
                     /locus_tag="IYE22_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506643.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
BASE COUNT         6268 a         3065 c         4059 g         5333 t
ORIGIN      
        1 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
       61 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
      121 aaaggataag tttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
      181 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
      241 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
      301 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
      361 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagca aatccctacg
      421 atcacaaaaa aaatgctgac ccagcagtta agagagttag aacaagatga aagtatccat
      481 cgaaaagcct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
      541 tcattaagag agattctagt agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa
      601 aaagcctcaa gggaagaaga cacgctactg catacacatg gtggctacct aaattattga
      661 ttttctatga catttatttt aaataaaaga aaacaggagt ttttttaaaa gcatcatcta
      721 ttttttatgt aaaaaaatgg tatgattaat caagtaagaa gtgattggaa gaaggcaagc
      781 aaatgaatag tatggaaaaa gtcaaccagt ttagagatga acgtaattgg cgacaatttc
      841 ataacgaaaa agatttggct atctctattt ctttggaagc atctgaattg ttagaacttt
      901 ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt agaacgaatc aaagaagaac
      961 tggcagatgt attgatctat tcatacatga tggcagacaa ccttcacctg gatctagatg
     1021 agatcattga agaaaaattg atcaaaaata atttgaaata tcctgtagaa aaaagcgcag
     1081 gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa
     1141 aagaaaaaca gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa
     1201 agatggctag tcaatatcct cttgttcctc ttgttattag taggactagc tttgattttc
     1261 aatgaacaga tcaaggatta ttttgtcaga gagacaggag ataaatatgc tatagcgaat
     1321 gtaacaaaag aagatctgaa aaagaataac gataaagatg tcagttttga ctttgatgcg
     1381 gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac agatctacca
     1441 gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt taccggtttt caaaggcttg
     1501 gataatacgt cactacttta cggagcagga acattatctc cagatcaaga aatgggaaaa
     1561 ggaaattatg ccttagcaag ccatcgtgca acgaaccctg aattgctgtt tacaccgcta
     1621 gaaaatttag agatgggtgc caagatctat ttgacagatt tagaaaatgt gtatacatac
     1681 aaaacatttt ttaaagaaaa agttgctcct acagataccc aattactgaa tgaagtcgag
     1741 ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg acgccgtcac gcgactagtt
     1801 gtacaaggag aactggaaag tgttacatcg ataaaagatg cgacagatga tatgagatct
     1861 gctttcaatc tagagacaaa aacattttag gatactggga cataagtcgt tacagattaa
     1921 aaacagccaa aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg
     1981 caaagaggtg catctctgat ttggttgttt tctagataga cttgtgttcc agttttttta
     2041 ttttttatat gaaaaggctt gcataaataa aaattcgatg gtatgatgat tttgaataag
     2101 aaatggattg ttactgtacg gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa
     2161 ttctaggttt ttttattttt cttgagaggg cgaagcaaaa tgaaaattga taaaattttg
     2221 aataacaatg tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa
     2281 ggactagctt ttcaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     2341 tttgttttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     2401 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     2461 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     2521 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     2581 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     2641 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     2701 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     2761 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     2821 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     2881 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     2941 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     3001 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     3061 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     3121 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     3181 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     3241 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     3301 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     3361 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     3421 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     3481 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     3541 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     3601 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     3661 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     3721 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     3781 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     3841 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     3901 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     3961 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     4021 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     4081 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     4141 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     4201 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     4261 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     4321 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     4381 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     4441 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     4501 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     4561 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     4621 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     4681 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     4741 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     4801 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     4861 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     4921 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     4981 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     5041 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     5101 caatcaatta gaaggggctt atgatcgaga cggaaaaggg ctatccgttg cagatgctat
     5161 gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact ggacgattga
     5221 tcaagaaaaa tatatttacc cgaatcatcg aggaattgat cattacgatc gtttcaaaga
     5281 agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga ttgcctggac
     5341 acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag aattttatga
     5401 tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga tttctcatta
     5461 cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa aactgatcga
     5521 cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag tcaaatattg
     5581 gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc aaggattagt
     5641 aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata atcaattcgt
     5701 tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc aaatcggctg
     5761 catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc aggcagcaac
     5821 aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac gtggagaata
     5881 ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa aattagagca
     5941 aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg gatttagtta
     6001 ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag aagggaactt
     6061 gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc agatcgatcc
     6121 agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac cattattcat
     6181 tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga ttattgatga
     6241 ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag tagcagacgg
     6301 tgtagatttg atgggctata cgccttgggg atgtatggat cttgtcagtg catcaacagg
     6361 agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag gaaatgggac
     6421 actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg cctcaaatgg
     6481 cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca tgtgatttgt
     6541 gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa gggtgacgtt
     6601 atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga agatattttc
     6661 tcaggaaaat atccagtcgg ttcgatgtta ccgacagaaa gtgaattaga agaactgttt
     6721 caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga tgaatatgtc
     6781 gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa tcgattatcc
     6841 aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg gaaagaaacg
     6901 ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt tttcggtgag
     6961 caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat ttattttgtt
     7021 cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa agaatctttg
     7081 tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga tttctcggca
     7141 gtatttttaa cagaagaaga acaagaaatt ctgaaaacag acgcacagat cggcatcaaa
     7201 cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga ggcggtctac
     7261 catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa agaggttgtg
     7321 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatattag
     7381 cttctcatat ttttcgtttg gttaggctta ttaccgcaga tacccggctg tttctggatt
     7441 accatttatt ctggataagg gggtgtggca cgattttcag aaggaatcta tgaaccaatc
     7501 actgtattga ttttagcttc gatggtaacc ccattcggct tgacgatcgc ttatttctta
     7561 gggaaaatca ttcgtaaaaa tatattcaat cgtcaagaaa tcgatacatt gaagaccgct
     7621 tttcctatgg ggatctgtca gatcacagag ggatgtatcc ctatcgtatt gaacgacttg
     7681 gcacgcaatg tcatagcaac gggagtcggt ggggcagtcg gcggtggatt gagcatgttt
     7741 tggggcgcag atagccgtat tccggcatcg ggaatgtttg cagtcgctac gatgaccaga
     7801 ccatgggcat tcattggtgc attgttagct ggttcttttg ctacgggaat aactatttta
     7861 ttattgaaaa aaccagttga cccaaatgca gaagtcatcc aagaagaaaa agaggaagaa
     7921 gagatatctt gggatgactt gacaatcagt taggaggaac agaatggatt acaagaaaag
     7981 agtacaacaa atgttcctag attcaggagt agtattgaaa gaaacagaat tagaaagaat
     8041 cgaatataca gattttggat taggagcaat cgagactgaa ggattgaatt tatttgttta
     8101 tgaaaataat gagcgatact gtgggaaaga actggtatta ttgccttatc aaacttgtcc
     8161 agagcatcgg cacccacctc gagatgagaa tgaaggaaaa aaggaaacct ttcgttgtcg
     8221 aaaaggctta gtatatcttt atgtcgaagg aaaagaaaca aaaaaacttt cctgtattat
     8281 tccaaagggg caagaacgtt actatacggc aaaaaaagaa atcatacttc acgctggaga
     8341 tcagtacacg atcgaacaaa atattaaaca ctggttccag gcaggtgcaa caggcgcggt
     8401 tatcaccgaa ttttcttcca gcagtgatga tccaagtgat agttttacga atccagctat
     8461 ttatcgttaa tacaaaggtt tgggaacaag ctgatgaacc aaaatgatca aatttcgatt
     8521 atttggttcg tcggcttttt tatttagaga aacatcaggc gttggtgaaa cataacacga
     8581 gaataaaatg gatttttcat taataggaga aacaagtgtg tgactattta aataatgcgt
     8641 gttattgaca tgcaggacga aatcccaatt tcttctataa gtaagtcgtt tcaattttat
     8701 atttactagt tatttatttt tttggttact gtaaaatagc tgaaaaatta tttttttgca
     8761 aatactatat atagtggtgt ttgatctata taccactaaa aaactacaaa atctagtctt
     8821 ttattttaaa aaaacacgtg tagaatgaag atagactttt gagttaggga gtgttttttt
     8881 atgaaactta ctgttttttg cggttcccgt tttggcaata aagaaagtta caaatttatt
     8941 gcgcaaacgt taggaaaata tatggcacaa gaagaaatcg aactggttta cggtggttca
     9001 tactccggga ttatgggagt gattagtaaa acagtactgg aaaataacgg aaaagtcaca
     9061 ggtatctatc caaatggcct ttttgaaact gagctgcctg ggaaagatgt tactacattt
     9121 attcctacag agacaattga tgaaagaaag gtattattat ttgaaaaagg agatgctgtc
     9181 ctcgttttcc ctggaggtct aggtacattg gaggaatttt cgcaacttct ttcttggata
     9241 gcgatcggcc tgacacctga taagccaatc ggaatattga atattggcgg ttattacagc
     9301 ggcttgcaac aattattaga aacttttgca aaagaggatt ttatggataa aaaatggttg
     9361 gatcaagtga tcttttccaa caatccattg gaactggtca atgaattacg agcagtatct
     9421 attaaaaatc agttattatt gaaggaggca aaataatgag tcaaatatat ttagcaggac
     9481 catttttttc agaggagcaa atcgatcgag taagtcgtat cgaaaaagcg ttggaggaaa
     9541 acaaaacaat cacaagtttc tactctccac gtcatcacca agaaagcaat tatgaactat
     9601 tcagcgctgg atgggcgcag gaagtatacg aaaaagatat ggaagagttg acaaatgcag
     9661 aatttgtttt ggctatcctt gactttgagc atcaaacaat cgatccagga acggcttacg
     9721 aattaggtgt ggcaactatg ttgaaaaaac cgatgatcat cgtacaagaa gagacggtac
     9781 ctacgaactt gatgattaca caaagtcttc acacgtattt aaaatcagac caagctgtac
     9841 gtgaatatga ttttgaaaaa ttgcctgttg aaacttacgt aggcgagtac ttatagaaga
     9901 ggaagggttg tatgtcagtg attttgattt caggaacgat tggtgcaggt aagagcagtt
     9961 tgacggatat gcttgcaaaa gagattgatt ctaaaccatt ttatgaaaat gtcgaagata
    10021 acgaagtatt gccgcttttt tattcaaatc ctgagcagta cgcattttta ttgcaaatct
    10081 tctttttgaa taagcgattt ttagcaatga aaaatgcttt agtgaacgat gacaatgtgc
    10141 tagaccgctc catctacgaa gatagtctgt tgttccatct aaatgctgat ttaggtcggg
    10201 taacggatat cgaagtacag cagtacgata gcttattaga tacgatgcta aacgagttag
    10261 atgatgttgc tccaaaaaaa cgtcctgatt tgatggtcca tatcaaagtt tctttggaca
    10321 cgatgctaga acgtatcaaa aaacgcgggc gtgactatga acagctagaa agcgatgaaa
    10381 cgttatacac ctattacgaa acactgaaca cccgctataa tcaatggtat gaggattttg
    10441 atgtatgtcc taaaatacaa gtggatgggg atacattcga ttttgtagaa aatccagaag
    10501 atgcaaaaaa ggtgattcgt cagatccaag aaaaattagc tgaattggaa ggaaagcctg
    10561 ctggaaaata ttttacagat aatcagcatg cgaaaggtca agaattaggc taaaagtaaa
    10621 aaaccaaacg acgggaaaga aaaattacta cgtgatgtag aataagtctt tctatcgttt
    10681 ggttatttta tattcaaaga tgtgaaataa ataaaacgtt actctttgat ttgtgaactg
    10741 aaatgatcca ctgtttgttt tactgattcc agtttttgaa gcttgatcaa aggaagaagc
    10801 tgagtttgaa gttcataaaa atcagccata gtgagttttg cttgattttg cttatcttct
    10861 tttgcttttt acataatttt tgacaggata agattcaaac gatttaaaag aatctttgac
    10921 tagtttgtgg caattgtcgc atatactcaa acttccttga tcattaaatt ctgtatggat
    10981 atttttgttg ttattacatg tacaataatc aggactatcg atcatccatt tcgtctcctt
    11041 tacagtcatc ttgcttcttt actatagcag aaaagaaggg gagaaacaaa agaagatcca
    11101 acagatggca ctatagactg tagatgttgc catccttatc cttcaaacgg taattaccga
    11161 ttttggtaaa tgtgactgtt gcttcttttt tattttcttt tcctgaaata atatattcgt
    11221 catctttggt agtaatcttg atatctgtca gcactgtttt ttcttcagat gattcagaag
    11281 aatcagctcc ttgtgtctga tacgtgcgtg tccattccat ttttttatct gtaaatgtca
    11341 actgtcccgc ggataaatcc atatctttac gtacttttag cgtatataca ctgttggtga
    11401 tatctgcttt ttccgctttt aagacagaag aatcggttgt ttcgtccgag gattggtcag
    11461 agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc cgcagacaaa ataaatagta
    11521 cgattaattt cttcaaaata cttcccccat ttgataggat aaagaaatta taccattgga
    11581 aagtaaaact tcaaacggat ggaagagaaa aaattcacaa aataccatta aatagaataa
    11641 tttttcaaaa gtcataaaaa atccaccacc tttagaaagc gttttaaaaa aatgtgttgc
    11701 aatctttaaa aaaagtgcta tattaaaaat gaaccggttc aattttagaa tctaggagat
    11761 gaaataatga aagtcgtaga cagactatct gaaaaaatgc taccaatcgt atctaagatg
    11821 agtagccagc gacacttgct ggccattcga gacagtttta tcgttactat gccgttagta
    11881 atggctgctt ctgtcatggt tttgctgaat gctctgattt tttcaaatgc aacagtccaa
    11941 agaatacttg atctaagtcc attggctgaa cttgcttcaa tcgtgaataa tggtacgatg
    12001 agtattttag ctattatcgt gtgttacaat atcggctgga atttggcaac ccactatatc
    12061 caaacaggcg tgattgatga tccatcattt agtcctgttc atgcaggaat tctaagtaca
    12121 gccgtaatgt ttattttgat gtctttatca agtacagtca ctttgatgga cggaaataca
    12181 gcagaagcta caggggtttt cttacaaagt ctgacttctt cgtctggctt agcaacggcg
    12241 atgattgctg cccttgtcag tacagaatta tttatcaaat tagcgaaaat gaagagtttt
    12301 aaaatcagga tgccagaagg agttccaccg gcagtagctg cgtcatttaa ttctttagta
    12361 ccagaaatag ttgtcatttt atttttttca gttgtcgttt ttactttaaa ccatacgact
    12421 ggtttgaatg ttccgcaatt aattgaatta gcgattcaga caccattgaa agcttttgtt
    12481 ttatccgtcc caggcatctt atttttacag tttttctctg atttcttatg ggtgtttggt
    12541 atgcatggtt cgagtatttt ggctcctata cggcaagcac ctttgttaca gtctattcag
    12601 gaaaacatgg atgctttttc agtaggaaaa gaaattccta atattattac ggagcccttt
    12661 accaatgcat ttggattgat cggaggtggc gggtgtattt taccgttagt tattgctatc
    12721 ttgtgggctt ctaaacgtca agaacagcga tcaatcgcta aatttggtct gactacttgt
    12781 ttatttaata ttacggaacc aattatgttt ggattaccag tagtcatgaa tccaatatat
    12841 atgattccat gcgctattat accatctatt aacttggtta ttgcctacgc agccacaagt
    12901 cttggaatca tcagtaaaac agttgctgct gcaccttgga tcactccacc ggttatacaa
    12961 tcttttattg ctacaggagg cgatatacga gcagcagtct taacagtaat acttatcatt
    13021 ctagatgtat ttttattcct gccatttgta ttagccgcta ataaggcaaa attggcagaa
    13081 ggaggatatt aaggtgtacg attacccgta tcaaaaccca aagttaacca tccgccaaag
    13141 agtagaagat ttactttcga ggatgaatat tgctgaaaaa gtaggacagg taaaccagca
    13201 tttgtatggt tgggaatgtt atgaaaaaaa cggagacaag atcgaattaa cagaaaagtt
    13261 aaaagaacat gttaaatggg gaggcggtct gggtgcctta tacggtctgt ttcgctctga
    13321 tccgtggtct aaaacagatt ataagaatgg gattcctgca aaagaaagct ggaaggtggc
    13381 taacctagtc caagaatatg tactacagca ttcgcgatgg aaaattccag ttttgcttgt
    13441 agaagaatgt ccacatggtc atcaaggctt ggatgggatt tcttatccaa cgaatatcgg
    13501 ccgtggaaat actttcaatg tagcattatt agaacaatcg agtcgattga tggcaaaaga
    13561 actggctgct aaaggtgttc atttagcgct ggtgtcgact ttggatctgg caaaagatcc
    13621 tcgttgggga aggacagaag agtgctttgg ggaagatccg attctctcag ctcgtttttc
    13681 tgaagcggtc gttcgagggt tccaaggaga gattatttca gaaaaagttt cttttcttga
    13741 tcagacagta gaggaaatca ataagaagaa ggatcagatc ggtgttgtgt taaagcactg
    13801 tatcgcacaa ggagaagctt taggcggtca taattcaggc acagtaacga ttggacgcag
    13861 agagttctcc gatatttacg gaccattatt gaagagtaca agaaatgcag taggtgtcat
    13921 ggctgcttat aatgacatcg acgggatacc ttgtcatatc aatcgagcgc tttttgagca
    13981 aacattaaga aaagagatcg gttatcaagg aatcgttatg gcagacggtg tggctttgga
    14041 tcgtttatcc gatgttttta cagataaaaa gactgctgca gcatatgcat tagcggcagg
    14101 aatcgatctc agtttgtggg atgagaccta taccaagatt gcagaagcca ttgataatca
    14161 agtcgtagat gaaaagctac tagatcaagc agttcgccga gttttatctg tcaaattttt
    14221 attaggttta tttgaacagc catttgcaaa cgatccaaag gagtattggg atcatctcat
    14281 ggaggaatca gagcacttaa atttagagac agcaaaagaa agtatcactt tactaaaaaa
    14341 tgacggtata ttgccgttgg atcaacaagt aaaaaacatt gctgttatag gtccaaatgc
    14401 tcacgatgtt taccacttgt tgggagacta ctctgcacca caaacagaag accgtttgaa
    14461 aaaaaagata gtacaagaga taaaaacaga gtttccccaa tcaaaggttt cttatgcaca
    14521 aggatgtgaa gtaagaaatc aggaggatca ggaagaaaaa ttattagaag cggtgaccct
    14581 tgctcaaaac tcagatgtga tcattgcagt tctaggtgga tcaagtgcgc ggaactttga
    14641 tatggaattt cttcgtaacg gggcagtttc atcgaaaggg atcaacatgg attctggaga
    14701 aaatgtagat gttgcttctc tttctttagg tggataccaa gaacagctga ttgaacaatt
    14761 gtatcaacta ggcaagccaa tcatcacgat acttgtgcaa ggaagaccct acgatttgat
    14821 gaaaatcgag tcatttacga atgccattgc aacagcatgg tttcctggac aggagggtgg
    14881 aaaagccatt gcgcagatga tttctggaag aaataaccca agcggacgat tgagtttgtc
    14941 ttatcctaga aatagtcggc aactacctgt ttattactat cagcgtgctg cttctaaaca
    15001 agagaattat tatgatttaa gcggaagtcc attttatcct tttggacatg gtctaagcta
    15061 tacaacattt gaatacagtc agatggaagt caaaacggat attgacaaag taataatatc
    15121 aattaaagtt aaaaatacag gaagtgtttc aggtaaaaca agtactcttg tctatgtccg
    15181 attaattgac ggcgttgtca tccaaagaac gaaattattg aaagcttttc gtaaagatga
    15241 attgcggtct caagaggaaa gactgcttgt ttttgaactc acttctgaag actttagtta
    15301 tatggatatt gatgacaagc ggcattatgc aaagaaagcg gtcatcatgg tcgaacaaca
    15361 agaaaaagaa atcaaatgta actggcagga gtgaaaatgt atggcatcga tccgtgacgt
    15421 ggctaagatg gctggtgttt cagtgggaac agtttcccgc tatttaaacg gtcagcagct
    15481 gaaggaaaag aatatggaaa aaatatcaga agcgatttca gcgttggact ataaagaaaa
    15541 catcattgct aaaggattga agaataatcg aagtttctct attggactgt tgataaacgg
    15601 gatatctaac cgatttggtt cggaggttgt ttcagggatc gaaagagtag ctgaagaaaa
    15661 tgggtatagc ttattattaa gcgggtttgc cgatcagcca gaaaaaatcg aacaaaaaat
    15721 cgaatacctg atgaagcatg cgatcgatgg attgatcgtc ttcttatcag aagaagagtg
    15781 gtctggattt gaaatgctga caaaaatgag tatcccagtt gtcgcattga attgtccggg
    15841 tgggccagca ggtattgata caatagtagt gaatgatcga gaaagtgtag caaaagtgat
    15901 tgctcatcat ggcggacaag gacataaaac aataggattt attgctgccg ctcaaacaga
    15961 ttacgtagca agagaacgtt tagctggagc caaagaagcg gtacaaaagg atccgtctat
    16021 ccatttagag atctttacag gagactattc tagaaaaagc ggctattacg gggcaaaagc
    16081 attattagaa aaaggggcaa ctgctatttt tgtcagcaat tacaacatgt cgattggtgc
    16141 catcgaatgt ttcaatcaag aaggtgtcag aatcgggaag gatattgcgt tcagtcacta
    16201 tgattattta gataaaaacg atctgagtat ccttccaaaa atcacgatca cgccaccaac
    16261 agataaaatc ggggaacgag cagcagaaca gctgttgaaa cgaatcaaaa aagaagagga
    16321 agaaaatgaa aaagtgattg tgatggaaaa tatcatacat ggaatcaact aaaaaagaaa
    16381 aagaggtgct ttgatgaaaa gatatcaatt tccaaaaaat ttttggtggg gctctgcagc
    16441 aagtggtcca caaacagaag gaagaatttc aggcgatgga aaaggagaga atatttttga
    16501 ttattggtac aaaaacgaac cgcaaaagtt ctttgatcaa gtaggacctg aaaaaacctc
    16561 gcaagtttat acgaagtatc aagaggatgt acagttgatg aaacagactg ggcataattc
    16621 atttcgaacc tctatccaat ggagccgttt gatacctgat gaatctggaa caatcaacga
    16681 agaagccgtt cgtttctatc atcactattt tgatgagttg attgaaaacg gtatcgaacc
    16741 ctttgttaat ctgtatcatt tcgatatgcc gatgtatctg caagaaaaag gcggatggtt
    16801 gaaccgagaa acggttgaag cctatgaaaa atacgcaaaa agttgttttc aattgtttgg
    16861 ctcgaaagta aaaaaatggt ttacccataa tgaaccaatc gttccagtcg aggcaggcta
    16921 tttgtatcga tggcattatc cggaagaatc agatatgaaa aaggcaatcc aagttggtta
    16981 tcatgaagcg ttggcttcag cattagccat caaagcttac catgaaatgg atcaaagcgg
    17041 tgtgatcgga atcatcttaa atttgacacc gagttatcca agagacgaat caaatcccga
    17101 agatgttaaa gcagcacgaa tagcagatgc gttcttcaat cgttcattcc ttgatccagc
    17161 tgtaaaaggc gagtttccca gtgaattgat tgagagaata aaagaactcg atctaatgcc
    17221 ggagattcat ccagatgatt tgacgatcat taaagaaaac acagtagatc ttttgggaat
    17281 taattattac caaccacgcc gaataaaagc aaaagaaaca ccaatcgata ctaaaaacgg
    17341 tcctatgcct gaggattact tcgacaatta tgaaatgcct ggtcgtaaaa tgaatcctta
    17401 tcgcggatgg gagatctatg aaaaaggaat ctatgatatt ttgatcaatg tacgagatca
    17461 ttatgggaat atcgactgtt ttatttctga aaacggcatg ggtgtagaaa atgaagcacg
    17521 atttatcaag gaagatggta tgatccacga tgattatcgg atcgaatttg ttcaatctca
    17581 tctgaaatat gtccatcaag ccattcaaga aggtgccaat tgtttaggtt atcatatgtg
    17641 gacatgcatg gataactggt cgtggaccaa tgcctataag aatcgttacg gttttatttc
    17701 agtagattta gcacaagatg gaaaaagaac gatcaagaaa tcgggtcatt ggttcaaaga
    17761 tgtatcagac agaaatggat ttgaagctta aagaatagcc aagagagaat gtatgaaaat
    17821 tcccgttctt accgcttcag gaaaaagaag ttcaattatt tacgatcttt ttcaactttt
    17881 ttgtctttct atttcatttt ttgtatataa tagaatagtc aaaattaaat aggagtggag
    17941 gaaggcatga ggaaaaaaag accgtttctt ctcttttggt tattcggttt tttcttactg
    18001 cttattttat ccgcagcatt ttcaggaaaa atgacagcag atcttgctca gaaagaaaca
    18061 gaaaaaatcg tagcaagcaa agaatcagta gataatttca aaaaagaatt ctcaacttat
    18121 tggtgggagg aatataaatt aactgccaat tacgaaaaag gtgaattgga catctatttg
    18181 ccaaaaggaa aagaagaaaa tcaatttatc aatatcgatg acgtacttag tgcgatttct
    18241 ctcagacaat atcaattcaa agacaaacat ttggtcgttt tgcttttgac tcatgatggc
    18301 gacaaggtag cacgtttgaa aaatggaaaa gtggttacag cttattctga aaaagtggac
    18361 aaaaaagtgg tggccgatcg actagcggaa tggaaaaaga ccaacgaaca agtcaagaaa
    18421 acaactgctt cttctcaaaa aaacgagtcg acagaactaa gcccttcaga ttctggcagt
    18481 ggacaaacga taacgggaaa tgaagaaaat caagcctcaa tcattacgaa tgagtcatca
    18541 gaaggacaat atcaaaccta tggacaatga aaaacagaag ttgtgacaga agtggtcagc
    18601 ttcaagaaat aagaagaaat ttacgaaaag tgcttctcaa gtttttgtaa aatttcggct
    18661 tatttccgaa gaagctactt ctggaacacc gtgaatcaga ggttgtgaaa tcagcggttt
    18721 gacct
//
LOCUS       DACIRY010000047        18594 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.048, whole genome shotgun sequence.
ACCESSION   DACIRY010000047 DACIRY010000000
VERSION     DACIRY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.048"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            189..836
                     /locus_tag="IYE22_001428"
     CDS             189..836
                     /locus_tag="IYE22_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ9506644.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1229..3874
                     /locus_tag="IYE22_001429"
     CDS             1229..3874
                     /locus_tag="IYE22_001429"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ9506645.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4184..5497
                     /locus_tag="IYE22_001430"
     CDS             4184..5497
                     /locus_tag="IYE22_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ9506646.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5487..6140
                     /locus_tag="IYE22_001431"
     CDS             5487..6140
                     /locus_tag="IYE22_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ9506647.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6195..6887
                     /locus_tag="IYE22_001432"
     CDS             6195..6887
                     /locus_tag="IYE22_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ9506648.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7037..7237
                     /locus_tag="IYE22_001433"
     CDS             7037..7237
                     /locus_tag="IYE22_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9506649.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7491..9170)
                     /locus_tag="IYE22_001434"
     CDS             complement(7491..9170)
                     /locus_tag="IYE22_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ9506650.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9172..9384)
                     /locus_tag="IYE22_001435"
     CDS             complement(9172..9384)
                     /locus_tag="IYE22_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ9506651.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9777..11507
                     /gene="efrA"
                     /locus_tag="IYE22_001436"
     CDS             9777..11507
                     /gene="efrA"
                     /locus_tag="IYE22_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ9506652.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11504..13264
                     /gene="efrB"
                     /locus_tag="IYE22_001437"
     CDS             11504..13264
                     /gene="efrB"
                     /locus_tag="IYE22_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ9506653.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13359..13556
                     /locus_tag="IYE22_001438"
     CDS             13359..13556
                     /locus_tag="IYE22_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506654.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13709..14194
                     /locus_tag="IYE22_001439"
     CDS             13709..14194
                     /locus_tag="IYE22_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ9506655.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14298..14891)
                     /gene="clpP"
                     /locus_tag="IYE22_001440"
     CDS             complement(14298..14891)
                     /gene="clpP"
                     /locus_tag="IYE22_001440"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ9506656.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15071..15598
                     /locus_tag="IYE22_001441"
     CDS             15071..15598
                     /locus_tag="IYE22_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ9506657.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15689..16426
                     /locus_tag="IYE22_001442"
     CDS             15689..16426
                     /locus_tag="IYE22_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ9506658.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16430..17347
                     /gene="miaA"
                     /locus_tag="IYE22_001443"
     CDS             16430..17347
                     /gene="miaA"
                     /locus_tag="IYE22_001443"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ9506659.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17349..18578
                     /gene="hflX"
                     /locus_tag="IYE22_001444"
     CDS             17349..18578
                     /gene="hflX"
                     /locus_tag="IYE22_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ9506660.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         6013 a         3217 c         4037 g         5327 t
ORIGIN      
        1 gtaataagaa cagccaaaca aaaatagttc ctcatatttt acgtttggtt gagcttatta
       61 ccgcagatgc aagcttttga acaccgtttt atttgtctca cacccttttc cttcctactt
      121 gtaaccttgc tcccgtgtta gaaacatgtt acactaaata tgtgaaatta ttaacaagga
      181 gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa agattacctt
      241 tatatttacg ctacttgaag atgctggatg actcaggtat aagtagaatc aaatcaaatg
      301 agttcagcga aatcacccaa attccttctg caacgatccg cagagacttc tcgcaattag
      361 gcgagctagg cagaagcgga tatggctatg atgtcccttt tcttatcgat gtattcaata
      421 atatccttaa tactaaagaa gaaaaacgaa tcgcattagt aggatatgga aatcttggca
      481 aagcattgaa acacaacaat tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg
      541 ataatgatcc agcactgatc aatcgagtaa ttgatggaga aatgatctat ccgattgatc
      601 gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc aatctctacg gtaccaagta
      661 aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt
      721 ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa
      781 ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa
      841 atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag
      901 tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga
      961 atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta
     1021 tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat
     1081 ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta
     1141 ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac
     1201 gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata
     1261 taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa
     1321 accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac
     1381 tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca
     1441 aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc
     1501 gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg
     1561 tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg
     1621 cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga
     1681 tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat
     1741 ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat
     1801 cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga
     1861 tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc
     1921 gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct
     1981 acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg
     2041 gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg
     2101 tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg
     2161 aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga
     2221 aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac
     2281 aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc
     2341 gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt
     2401 caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt
     2461 atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg
     2521 gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg
     2581 gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga
     2641 ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg
     2701 ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt
     2761 cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa
     2821 tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt
     2881 atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc
     2941 ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg
     3001 aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct
     3061 tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg
     3121 tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga
     3181 aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat
     3241 cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac
     3301 agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc
     3361 tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga
     3421 attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat
     3481 tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag
     3541 ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc
     3601 agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt
     3661 gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga
     3721 agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga
     3781 agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa
     3841 agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca
     3901 gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca
     3961 ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc
     4021 tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag
     4081 ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat
     4141 gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc
     4201 aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga
     4261 agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg
     4321 aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct
     4381 gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa
     4441 tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat
     4501 caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc
     4561 aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt
     4621 aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat
     4681 tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc
     4741 tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac
     4801 tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta
     4861 taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga
     4921 aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc
     4981 aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga
     5041 aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag
     5101 taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt
     5161 tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg
     5221 aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta
     5281 tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag
     5341 tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat
     5401 gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca
     5461 tttattaata caattaggag gagatcatga agtataatgc agtgattttt gacatggatg
     5521 gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga
     5581 tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag
     5641 tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca
     5701 tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc
     5761 ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt
     5821 ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat
     5881 ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca gaaattttct
     5941 tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac
     6001 aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc
     6061 ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa
     6121 tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga
     6181 aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa
     6241 gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct
     6301 tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa
     6361 cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag
     6421 ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc
     6481 agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat
     6541 tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga
     6601 agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc
     6661 atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc
     6721 ataatcatcc ttctggtaat gttattccat cgcagcagga tatgaatttt accaaaagga
     6781 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
     6841 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
     6901 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
     6961 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
     7021 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
     7081 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
     7141 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
     7201 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
     7261 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
     7321 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
     7381 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
     7441 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
     7501 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
     7561 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
     7621 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
     7681 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
     7741 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
     7801 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
     7861 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
     7921 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
     7981 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
     8041 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
     8101 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
     8161 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
     8221 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
     8281 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
     8341 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
     8401 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
     8461 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
     8521 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
     8581 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
     8641 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
     8701 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
     8761 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
     8821 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
     8881 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
     8941 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
     9001 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
     9061 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
     9121 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
     9181 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
     9241 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
     9301 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
     9361 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
     9421 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
     9481 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
     9541 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
     9601 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
     9661 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
     9721 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
     9781 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
     9841 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
     9901 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
     9961 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    10021 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    10081 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    10141 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    10201 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    10261 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    10321 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    10381 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    10441 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    10501 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    10561 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    10621 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    10681 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    10741 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    10801 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    10861 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    10921 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    10981 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    11041 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    11101 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    11161 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    11221 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    11281 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    11341 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    11401 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    11461 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    11521 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    11581 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    11641 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    11701 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    11761 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    11821 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    11881 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    11941 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    12001 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    12061 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    12121 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    12181 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    12241 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    12301 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    12361 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    12421 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    12481 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    12541 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    12601 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    12661 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    12721 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    12781 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    12841 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    12901 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    12961 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    13021 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    13081 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    13141 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    13201 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    13261 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    13321 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    13381 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    13441 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    13501 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    13561 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    13621 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    13681 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    13741 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    13801 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    13861 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    13921 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    13981 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    14041 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    14101 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    14161 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    14221 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    14281 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    14341 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    14401 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    14461 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    14521 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    14581 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    14641 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    14701 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    14761 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    14821 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    14881 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    14941 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    15001 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    15061 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    15121 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    15181 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    15241 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    15301 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    15361 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    15421 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    15481 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    15541 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    15601 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    15661 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    15721 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    15781 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    15841 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    15901 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    15961 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    16021 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    16081 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    16141 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    16201 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    16261 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    16321 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    16381 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    16441 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    16501 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    16561 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    16621 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    16681 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    16741 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    16801 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    16861 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    16921 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    16981 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    17041 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    17101 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    17161 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    17221 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    17281 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    17341 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    17401 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    17461 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    17521 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    17581 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    17641 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    17701 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    17761 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    17821 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    17881 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    17941 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    18001 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    18061 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    18121 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    18181 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    18241 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    18301 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    18361 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    18421 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    18481 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    18541 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DACIRY010000048        18486 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.049, whole genome shotgun sequence.
ACCESSION   DACIRY010000048 DACIRY010000000
VERSION     DACIRY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.049"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..471)
                     /locus_tag="IYE22_001445"
     CDS             complement(<1..471)
                     /locus_tag="IYE22_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ9506661.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKN"
     gene            complement(629..1471)
                     /locus_tag="IYE22_001446"
     CDS             complement(629..1471)
                     /locus_tag="IYE22_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9506662.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            1603..2256
                     /locus_tag="IYE22_001447"
     CDS             1603..2256
                     /locus_tag="IYE22_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ9506663.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(2319..2846)
                     /locus_tag="IYE22_001448"
     CDS             complement(2319..2846)
                     /locus_tag="IYE22_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ9506664.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(2866..3813)
                     /locus_tag="IYE22_001449"
     CDS             complement(2866..3813)
                     /locus_tag="IYE22_001449"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ9506665.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(3825..5711)
                     /locus_tag="IYE22_001450"
     CDS             complement(3825..5711)
                     /locus_tag="IYE22_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9506666.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(5871..7949)
                     /locus_tag="IYE22_001451"
     CDS             complement(5871..7949)
                     /locus_tag="IYE22_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ9506667.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(7962..8237)
                     /locus_tag="IYE22_001452"
     CDS             complement(7962..8237)
                     /locus_tag="IYE22_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506668.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(8599..9054)
                     /locus_tag="IYE22_001453"
     CDS             complement(8599..9054)
                     /locus_tag="IYE22_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506669.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(9208..10416)
                     /locus_tag="IYE22_001454"
     CDS             complement(9208..10416)
                     /locus_tag="IYE22_001454"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ9506670.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(10528..10860)
                     /locus_tag="IYE22_001455"
     CDS             complement(10528..10860)
                     /locus_tag="IYE22_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ9506671.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            11008..11883
                     /locus_tag="IYE22_001456"
     CDS             11008..11883
                     /locus_tag="IYE22_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9506672.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(11965..12546)
                     /locus_tag="IYE22_001457"
     CDS             complement(11965..12546)
                     /locus_tag="IYE22_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ9506673.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(12552..13811)
                     /locus_tag="IYE22_001458"
     CDS             complement(12552..13811)
                     /locus_tag="IYE22_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ9506674.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(13813..14859)
                     /locus_tag="IYE22_001459"
     CDS             complement(13813..14859)
                     /locus_tag="IYE22_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506675.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(15094..15369)
                     /locus_tag="IYE22_001460"
     CDS             complement(15094..15369)
                     /locus_tag="IYE22_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ9506676.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(15612..16922)
                     /locus_tag="IYE22_001461"
     CDS             complement(15612..16922)
                     /locus_tag="IYE22_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ9506677.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(17104..18333)
                     /gene="rpsA"
                     /locus_tag="IYE22_001462"
     CDS             complement(17104..18333)
                     /gene="rpsA"
                     /locus_tag="IYE22_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ9506678.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         5153 a         3905 c         3101 g         6327 t
ORIGIN      
        1 gtttttcagt ggctttttaa aggaattgac cgttaacccg aataaattat tttggataac
       61 tttcatcaaa tcattaccgc caacagtcag tgtgataaaa tcagctgttt tgatattgtt
      121 tcgaatttct tcttttgttt ttactcgctt taagatctgg tcacttcgtt caccatttac
      181 accatagttt tctgtttcaa ccgcggtcaa attataacgg tcttttagat catttgcgac
      241 aaggggaacg aatcctcctt gcttggtttg atctcctact ccttcagtca gcgaatctcc
      301 gatagctatg tagtgaagtt tttctttttt atttggatct tctctcacag tagttactgg
      361 tttcattagt gggccagctt tcggtataga agcagctaaa cctgcataga tgatgatcgc
      421 aattaataat gggaacaaaa cgaggatcaa atgtcgtttc gttaatttca ttttttcacc
      481 tcttgtattc gattggttct aatgttatca aaaattttat caaaaaggta gtctttatga
      541 aaaaagaaaa caattatttt cctaatttta taaagaaaaa gagaataagc tgcaagcctt
      601 tcaggctgag ggcttatcct cttttcgact aatctacata atacatgatt gcaaacgcgt
      661 ctttaccagt atgcgtagcg ataaccggat tcgtatgcag tacagggata tgcatgtccg
      721 ggaataattc ttgcaaaccc tctttgaatt cttgcgccaa ttcttttgca tccgcatgtg
      781 aaataccgat ttgtcgcaca tttggcaatt ctttcagttc atttttcaaa ttattaaacc
      841 atttatcgaa cgttttcttt cctcgcccct tagttacagg gattaattca gaatgggcga
      901 aattcatcac cacgcgtatg ttcaaaacac tagacagcaa accagttgtt cggctgattc
      961 ttccaccttt taccaagtta tctagtgtgg atattccgat gtataatttt gtattgtctt
     1021 gtactttttt gacttcttca aggattttct ctactgtttt accttctttt gctaattcag
     1081 ctgctcgaat tacctgaaaa gataatcctt ggtcagtggt atcactgtcg ataaccgtta
     1141 ctttcgtttt tgttagatta ctggcttgtc ttgctgcttc tactgttcca ctcaagcctt
     1201 ttgtcatatg gatcgaaatc acttcactgc cgtcttctcc tagctcatca tacatcgaga
     1261 tgaattctcc aattggtggt tggcttgttt tgggcagatt ttttgctttc gccatcattt
     1321 ccataaatct ttcgccgtct agttggtcat catcgggata tactgttccg tcgatcatga
     1381 tggatagcgg catgacacga atgcctaaag catcgcgtac atttttttcc atcgtacatg
     1441 aagaatctgt tacgattttt acatttgtca ttattttctc tctctctttc tttaacaaga
     1501 tgccttattc atgttaaact acatgtaaga caatttctat ttttagtata acagacaaat
     1561 aatgattttt cattaattac acctagtaaa ggagccgttt tcatggaaaa aacgaaattt
     1621 aatcgaacgt atgaaatatt aaatgaagtg tttaatgcag tcacccatgg aattgggact
     1681 ggattgagta tcgcgggact agtggttctt ttagtaaaag gtgcccactt gcattcctcc
     1741 ctccatattg tctcttatgc tatctatgga tcgatgatga ttcttttatt tttaacgtct
     1801 actttgtttc attcattgat ttttacaaaa gcaaaaaagg tgttccaagt attcgatcat
     1861 agttcgattt ttcttttgat tgcaggaagt tatactcctt tttgtttgct gagtatccga
     1921 ggatggctag gttggacttt atttgtatta atctggttgt ttgcgattct tggcattata
     1981 tataaagcgt tgacgttgca taaacgggag agagtatcaa aagtatctac gatcatctat
     2041 atttttatgg ggtggctctg tgtcgtggct gctgttcctc tttatcactc tcttggaatg
     2101 actggtgtta ttttgatggt agcaggcggg ctttcttaca cgataggcgc attcttttat
     2161 agcttgaatt ctatccgcta tatgcatgtt gtttggcatt tgtttgttat gttaggtgca
     2221 ggttttatgt acttctctgt tttattcttt acttaataaa aaaaataaag ctatggatgg
     2281 gacagctcta aataacagct agtctatcca tagctttttt atatcgtcaa ttcgcctgtc
     2341 ccgcttttta caggattctc attacgctga tacgtttcaa attgatgcgg ataacgattt
     2401 ttttcatcca tttctccttg actagtattg atcagtgacc aatcagacca gtcaacaggt
     2461 ggaaaataag catctccatc gaatacatga tggatgactg tacggtataa tatcgtacaa
     2521 aatggcagaa attcttgata aacagctgac cctccagcga tgaaaaagat accgtctgtt
     2581 tctttttcca gagcaagcac gtcttccaca ctatgcatga catgcacgcc ttctgcttta
     2641 taggaagaat ctctcgtcaa aacaatcgtc tgacgattcg gcaacggacg gctacccatc
     2701 ccttcgaatg tttttcgtcc catcactagt gtatttgctt ctgtcatttg tttgaagaat
     2761 ttcagatcat ttggcaaacg ccaaggaaga cggtcttctt ttccaatcaa tccgttttca
     2821 tcctgtgccc aaattgcagc taacattttc tcatcctttc tgattctaaa cagcgatagg
     2881 agctttgatc gccggatgtg ggtcatatcc ttctaattgg atatcttcca tctcaaaatc
     2941 aaatactgac tgcttttcct gattcaaaac taaagtcgga aatgcgcgta tttctcggct
     3001 caattgttct ttcatttgtt cgatatgatt gatataaaga tgagcatctc ctaatgtgtg
     3061 aacaaattcg ccaaccgcta aacctgtttc atgtgcaatt aggtgagtca gcaacgcata
     3121 acttgcaatg ttgaacggaa cgcctagaaa cacatcccca ctgcgttgat acaattggca
     3181 gcttagccgt ccctcttgaa cataaaattg aaacatcgta tgacaaggag gcaaagccat
     3241 gctaggaaca tcttccggat tccatgcaga aacgatcaat cttctagaat caggagtctt
     3301 tttgattgct tcgatcactt gtttgatttg atcgataaag ccaccgtctt ttgtttccca
     3361 gtgacgccat tgatagccgt agatgtgtcc aagatcgccg tatgtttcag caaaggaatc
     3421 atcggttagg atcttctcgc aaaaaagctt gctctgcttt ttatattctt cattgaattc
     3481 aggatctgtc aaacatctat ggccaaaatc agtcatatct ggtccattat agtctggact
     3541 tttgatgtag cgttcaaatg cccattcatc ccaaatatgg tttttatttt gtaatagata
     3601 gcggatattc gtatctcctt ttaaaaacca aagcaattcg cttttgatca atccaaaagg
     3661 tactcgtttc gttgttaaca aaggaaaccc tttttgcaga tcaaaccgca tttggtatcc
     3721 gaataagctt aatgtgcctg tgcctgttct gtcttcttta cgatgccctt cttccaacaa
     3781 ttttcttcct aagtcaagat acgcttgttc catccttttt cctcctaatc ttctacccat
     3841 tcactcaagt attcccaccg tgccattttt tcttcaagtt gtgcttctgt ttcagatacg
     3901 tcgttttgca attcttgtaa tcgagtgaaa ttatctcctt gatgattcat ctcttcttgg
     3961 agcaatgaga ttttttcttc aagttctgca atctcatctt caatcgtttc ccattctttt
     4021 tgttccatgt aagataattt cttcttttca gcaggttcct tgctctgtat ttttggtttc
     4081 gctttttcag caggctgctg ggtctctttt tgcttcagta aataactgga catgtttccg
     4141 taataaagtt cttgttttcc gtttccttgg aatactaata acttatctgc taccttgtcc
     4201 aagaaataac ggtcatggga aacagtgatc acagcacctg gaaaacttct aaaataatct
     4261 tccaaaatcg tcaatgttgc tatgtccaaa tcattggtcg gttcatccaa taataaaaca
     4321 ttaggttgct gaatcagcag tttcaacaaa aataaccggc gtttttctcc gcctgaaagt
     4381 ttgcctatca gtgtcccatg cataaatctt ggaaataaaa atcgttcaag taattctgct
     4441 actccgattt gactgccatc tgctgttttt gcttcttcag cagcttcttg taagtaagca
     4501 atcatccgtt tatctggtgc catttcttca ttttcttgtg tataatacgc aagatgaacc
     4561 gtttcaccga tagacattgt accgctatcc aggggaattc tgccagctag aatattaagc
     4621 aatgtcgatt ttcccgcacc atttttacca gtaataccta gtctttcccg agattgaatc
     4681 aataaatcta aatgttctag cagtgtttgg tcatttatac ggtaactgcc atctttgatt
     4741 tccagtactt ttttccctaa acgttgtgtc gctaggttca gctctaaatc atcttcttgg
     4801 tttacttggt acaagttctc ctttagatct tcgaaacggt tgatccgtgc ttgctgcttg
     4861 gtgccacgtg cctgtacgcc agccctcatc caagctaatt cctgtttata aagccgcttg
     4921 cgcttttctt cttgttctac ttctacacga tcacgttctg ctttttctaa gacgtaagct
     4981 tcataatttc ctttatactc gtataatttt ccaaatgaaa gttcgaagat tcggtttgct
     5041 actctatcta gaaaataacg atcgtgggta accatcaata gtgcgccacg gtattgtttc
     5101 aaatagtttt ccaaccactc gatcgcttca taatctaaat ggtttgttgg ttcgtctagt
     5161 aatagtaaat cgggttcatc aatcaacact tgcgccagac taactcgttt tttctgacca
     5221 cctgataaag tactgatttt tttatctaat tcactgattc ctaatttttg caaaataatc
     5281 tttgcgttgg tatctgtagt ccaagcatct tctttattca tttgttcttc tgctttagca
     5341 taacgttttt gcacatcagg gtcttctccg ttttccccta gttcgatcaa tgcttcctca
     5401 tatgctcgaa cagtttggat caaggggctg tttccttgaa agactgcttg gagaacagtt
     5461 aattcatcgg aaaattcagc tgcttgagaa agataaccaa tacggtaatc agttggataa
     5521 aacactgttt gccgatcacc gtcgctgcta tcgataccgg caataatatt taagagactt
     5581 gttttacccg tcccgttcgt cccaatcaac ccgatacggt ctttttcgtg gaccaaaaaa
     5641 gaaatctggt caaacaaatc tttttcacca taagtttttt tgaggtcagt tacttttaat
     5701 tctttcaaca tgttccctcc ttatcttgga tattgtatca taaaaatagt gaacttcagg
     5761 aaaatttttt tggtatttgt tctttttgtg ttctcaaaaa aagaacagga ccgattcgat
     5821 cctgttctga tatgttttgt tttttataaa acttgaaaat ttttaccttt ttattttatt
     5881 ggactcgttt tttttgactt tgctcgaaac gcaaatcgga aagaaaagtt atcagatggt
     5941 ttgttcatcg tataagattc taacaaattg atattcctgc gatagctgtt cgcagaaaac
     6001 gtcgtgattt tttcagcaac ttcgtattca tcaatgattt ttctttcaag ataataacct
     6061 cttagcattt ttttcgtaaa cagactatct tgctggatca tccacgaaga aatcaaattc
     6121 cccgagttca tttttattcc ttcaagtttg cgttgctgga taaaaaattg aaccatcgtt
     6181 tcatcataaa tatcagtcaa gttatctaaa ctttcaataa tcaactgagt atttttttga
     6241 tatgtctctc tgatttcttg gatatcttca tcactaataa atgtctgacg atcagcatat
     6301 cttctttgcc aaaacatttt cggatggaga acgattcgca ttaacctacg gacaacaatg
     6361 aaccaaaagc cgataaaaga caaaatctgg cttgtgatag aatgttcgaa tcgagaaaga
     6421 aaacgggagt aaaaacgata accattagaa gaaagcttgc ccgaacgata actttcatcc
     6481 aatccatccc gttcgatcga caaaatcagt gcttgtattt cttgtacttc ttgcttttca
     6541 tcatcggtta aatcttccaa gtacagatca cgtatccgtt cttgataatt ttctatcact
     6601 gcttcagtgg ctaaaaattc ttttgtatta cgatcaattt cctttatttc ttttcgcaaa
     6661 acttcaacga cctcttctaa aatcatcaaa gcattcaaat ccgtgctttt cgcttcctct
     6721 gtttccgtca gcataggcaa gacaatgatc cctatgacca aagtcactaa gatgacaccc
     6781 gccgtcagaa acaaaagcaa agaccgctgg taaaacatca tattattgat tgaaaatggc
     6841 aaaataaaaa tcgtagccaa actgactgtt ccctttacac cgccaaatgt taagatcaag
     6901 atctcgttca actgtttttt gaacttttta ctgccatctt tgaatacata aaaaagactg
     6961 atagaaatga atctgatcac aaaaagcatg acagagatta atacgataat cgctaataaa
     7021 agaccgtttg gatataaacc gtctccccat actggcgaga acacttgcgt caattcgatt
     7081 cctaaaaata taaatactaa tgcattcagt gtaaaagcaa tagttgtcca tgtactgtgg
     7141 gacaagctcg acaattctgc atcaaacaca gagattttcc taaaaccaga agcttggaga
     7201 atacctgctg ccactgcagc aatgattcca gatacatcaa atgcctctgc caatacatag
     7261 gctagaaaag gaagcaaaag ttcgatcagc agataccctg tgacatcttg tgctgatgct
     7321 ttttcgatca aatgaattat tttttgtttg aaccagacaa gcaaaaagcc gatcaaagct
     7381 cccccaatac tagaaactac tagactcatc cccgcgttta ctgctgaaaa agatccggtg
     7441 atcagcgcac caagagcaaa ctggaaggca gtaacaccag atgcatcatt caaaagacct
     7501 tctccttcca aaatatgcat cgccttttta gggatattca ctcgtccaga aagagaactg
     7561 actgctaccg catctgtcgg acctaacgct gctccaaaag caaagcaggc agcaagtggg
     7621 acacttggca acaggaatga taacgtcgca cctaccccga caagggtcaa aatgactcct
     7681 ccaaatgcca agaacaaaat tgtcccgaaa tttttgagaa tcgaagaaat atctgctttt
     7741 tctccttctc taaataacaa tggtgcaata atcataacta agaacaattc aggttcaaaa
     7801 tcgattgatt gtccccactc tgtcagacct aataccactc caaggaaaat ttggatcagt
     7861 ggagctggaa tcattggcaa aatgcggcta gctatgttcg ataaggtgat cgttactgca
     7921 aatacaataa ctaactcagc aaattccatc gtccatcccc tttatttttt attttgtgta
     7981 agcattttta aaaattgttc ttttttcttt aaaattttgg tttcgactac ttcaacctgt
     8041 gtttggttcc cctctaattc acactgtaca cgttttgact ccaaacgaag cagatcatgt
     8101 ttcagacgtt ccaactgacg atacatcaat atatttcttt tacgcaactt acaaagtatt
     8161 tgacggcggt catccatcga aagggcgcca tagcgatcat gcaacagttt tttttgttgt
     8221 tcaaaactct ctctcatttt ccagatctcc tatacacaaa cttccatcat tatagcaaag
     8281 ttctttttgg atgaaaaata tttcattgtt tcatcgttta ttgctagtta gaaatttcca
     8341 taaatttttc aacgcctcat tctattccaa atagtatcag atatcaagta aaacttaaga
     8401 aagataaaag tttctgacaa aaggatgttt actttcccat tcctgccttt cttgttcttt
     8461 tatcacaaca aaaaaatggg gctgcgacaa aaatcttgtc acagtccctt attctgaata
     8521 aacggtgtgg aagagacagc ctatcagtag taataagaca catctctttc acagcctcat
     8581 ttttgcgtaa aaatcgattt attcgaaaaa ttccccgtat acatcttcta cacgttgatc
     8641 tggattgatc acgatataca agttacctgt tttttcgctg atttttgttg attgatattg
     8701 attgtctaat ccttctgttc ctttatctag atatgggaag tacacaagat caggtgtgcc
     8761 atttcggtaa aggatttcca ttcgctcgat atcttcgtat ttcaatgcgc gattaaacat
     8821 gcccatttcc agtccgccta ggttgatatc atgtgtcgca actgcgtccc cttcatggaa
     8881 gatttcgatt ttaaatccag cacacggatg gatttcaaca aattcactgc cattgattcg
     8941 tccaaaactt gttgtgattt gtttgatcca aagatcgccg atatgacggc ggttgatcgt
     9001 ccaggtttct ccatttctaa aataaaattt tagcgctgtc atttctttac tcattaaaaa
     9061 gactccttta tgatgtaatt tattcttgta ctacctcagt ttagcacaaa tttatgtaag
     9121 tggtcacatc ttatgttttt tcttctaatg acgtatctgc ccactttaag tagccaacaa
     9181 agctgagcag atacagtcta gtgtttttta aatcaatttt tctttcgata aaaaatccaa
     9241 aacctgtttt ttatcatttt ttattctttg ttctacgacg gctttttcag ctaattgcaa
     9301 agcttcacta acccacttac caggtttttt tccagtttct tctagcaaga ttttaccatc
     9361 tactgctaat tcgcttaatg acttgatagg taaacgatca taggattctc ctacggcatc
     9421 catctgaggt gtttcgtcaa aatagatcat caattcttct acttgcagtg catgtttttt
     9481 gcctaagaag tacaattcga atggttccaa agctccagtt tctcgttgtc gtagtccttg
     9541 gacaagtgct tgaacgtctt taatcatttg gttagaacac ttccacgatt tcaaaaagga
     9601 acgaatctca ttttctttta gatccagttg gctgatcaat aatgcccatg cttggctctc
     9661 ttctttcaat gggacggatg gtaattcagc aaaacgtaaa agtgcttcgc cttcttcttt
     9721 caaaccggga cagcaaatgt agcactctgt ttcaataaat gcatggattc ccgcggcccg
     9781 ttcatttccc aagagcatct tgatgaattc cacatttata cgttctacag atattttacc
     9841 taatagctcg tggttttctt tgatggcgta gaaggtttct tcctctaatt tgaaccctag
     9901 ttgactgaca aaacgcaacc cgcgcatcat tcgtaaggca tcttcatgga accgctcatg
     9961 aggattgcct acagctcgca agatctgttc ttctagatct tgcagaccat cgaacaaatc
    10021 gatgatttct ccatcttctt tcaaagcaaa cgcattgatc gtaaaatctc ttcttttcaa
    10081 atcttctgcc aacgaacgaa cgaattcgac atgatctggt ctacggtagt cctgataagt
    10141 tgattccgtc cgaaatgtgg tgatctcata ttgttcatca ttttctaata ccaacacagt
    10201 tccatgttcg atcccaatat ctatcgtttt tggaaaaagc tgtttgattt cttctggaaa
    10261 tgcgcttgtt gcgatatcca catcatggat tggctggttc agcaagatat cacgaacact
    10321 tccacctaca aaataagctt catatcctgc attctctaat tttttcagaa ctggaattgc
    10381 cttttggtat tccagaggta attgttttaa tttcattttt ccaacctttc ctgcgtgttt
    10441 cttgtcctga gataaagact cagttgcatg gtgactgttt gacaagtccg tttcagagtc
    10501 ttcaaggcca ggaccacttc atcttgttta ctcgtcctta cgctcaaagc gattatggtc
    10561 tctttgattg aatttttcca tgtttttttc aaaaacttca gttaagtcga tattcaatga
    10621 attagccatg atcatcgtaa cgaataaaac atcaccaagt tcttctgcta ccgagttaac
    10681 tggttcagaa gcctttttgc ttttttctcc ataagtgtgg ttgacttctc ttgccaattc
    10741 acctacttct tcagttaatc gagccatctg agctaatgga gagaaatacc ccaccttgaa
    10801 ttgctggata tattcatcca cttcctgctg catactgtat aaagaacgct ttttatccat
    10861 ctttttccct ccaaaactat cttaccaaaa ctttaacgca aatcctagat aagagcaatt
    10921 gagaaaatcg aatttccatg ctacatttag gaaggaattt ctaaatggaa attctgtgaa
    10981 aatacataat tgaaagctgg aattcatatg gtcaaacgaa aaacagtaaa agaagttctt
    11041 tttatcatta taggaacaag tatttatgca tttggtcttg tttacttgaa catcgctaat
    11101 catttagcag aagggggagt ctcagggatc accttgatct taagagcttt atttggtatt
    11161 gatccagctt atacgacttt attaatcaac atcccattga ttttactagg aggaaagatc
    11221 ctcggtaaac gttccttggc ctatactgtt ctaggtacac tttctttgtc attcttttta
    11281 tggatctggc aaagggttcc actagaaatc aatttacagc atgatctact gatcgtttct
    11341 ctacttgcgg gtttagttgc aggtgtagga agcgggatcg tctaccgcat gggtggaaca
    11401 acaggcggca gtgatattat cgctcggatt ttagaacaaa attacggcat cactatgggg
    11461 cgttccctat tggcatttga tatcatcgtc ttacttgctt cattaaccta tatcgacttg
    11521 aaacgtatga tgtatacgtt gattgtttcg tatgttttca gtcgtgtgat tgattcgatc
    11581 ttagatggcg gatattctgc aaaaggtcta cttgtcgtct ccaacaaaag cgaagagatt
    11641 gcaccactac tgatgaccgg tctgcaaaga ggtgtgactt tcctttctgg agaaggcgca
    11701 ttctctggta attcaaaaaa aatcatctat atggtcgtta gctctagtga attaactgaa
    11761 gctaagagga tcgtccacga gattgacgaa aaagctttcc tttcaatcat caatgtgcat
    11821 gaagtggaag ggcaaggctt tacttatttg aagccacaga caagattact gaaaaaacta
    11881 taagaaaaaa gacaaagata ggctatgagg aaagattcca catccagctg acctttgtct
    11941 tttttagtac tatactattt ttaactaatt gttatgttca cttacccggt tacttttctt
    12001 ttgtgtctgc aaaaggatct ttcggttaac ttcatcagat aatttcaaaa aagcatccct
    12061 atctccatct tctagtgctt gatcgatttg agcataaagc atttttagtt ttgcctcttc
    12121 ttctttttct tgaaaatatc gttcaattaa ttcagtatct tcttcactga ctgaagaatc
    12181 ccaagaagaa aatggattgt cttctaagat agatagatat tctcttgtct gccaagcatt
    12241 ttcgaatata cattccacat ataaaggctt tttccaattc aaacggattt catgaaaaat
    12301 ctggtctgtg tccgtaaatt cttttccctg caaaaacaat ttcatgggtt ccccttcaac
    12361 agatagatca ctgatctgca gtcctcgatc cgcaaaattc gccccttcaa caaagtggac
    12421 attgcttaaa atcgcttcat gattggcaag ataattcaag atccaaaaaa cttctcttcg
    12481 gctaaaagat acatgattga ccatccacgt taaaaaattc ttcttttccc gtacatcaat
    12541 tagcattttt ctcacctctc tgttaggtca tctaataatg acagtgcctc catatctcct
    12601 ggttcaagtg ttaaataata agtcaataat tcttgcgctc ttttcagctg cccttcttct
    12661 cttaagaaaa aggcatactc tttcaagaaa tctggttcat gtgatagttc atgataagcc
    12721 tgttcatagt gtacagctgc aacggtaaag tcttctaatt cattataagc atgggctagg
    12781 ttccattctg cataaggatt agcagtttct tccatctgat tgatcacttg tatcacatct
    12841 tcatatcttt catcatccaa gtaaagatta cttaaagtaa gtaatgtttc atcttgtttg
    12901 tctcctactt ctaaagcttt cagtaaaagt tcttctgctc gctgtctatc atgcagacgg
    12961 aaagcatttt ccgaggccag gtgataaagt tcgacttggt aaggattttc tttgattcct
    13021 tcttctaaca ctgtttgtgc ttcttcaatc atttcttctt cttgaagagc ttgtcctaga
    13081 tatagatata acgattgata atgcggatta acttctcgaa gttcttccag atagttgatt
    13141 gcctgctgat tttctttcaa ttgaagattc gtaaaagcta gctggaacag tcgatcatct
    13201 gtcctttctt ctttcagtgc ttcttctaaa ggtacgatag ctgcttcgaa ttctccttgc
    13261 atacttaagg attgtcctaa acgttcctgc atcgagattc ctgaaatttc atttatcccg
    13321 cttgccaaca atgtttcata aaccactgct gcctctttga aacgatcaac tgaaaaatac
    13381 aattcagcta gcgcaaactg gatcaatggt tcatcaggaa gcaacccagc tgcttctttc
    13441 aattttgctt cactcacttc agggattccg ataacttggt acaaatcagc tatcgccaat
    13501 aagctttgcg gatagtaatc actttgctta tccacttttt ccaggtattc aaaagctaag
    13561 tcgatctcat tatcttcgat tgcaatttct gctaacggga tgttcagtcc gtcattttct
    13621 gggtattggt tgatcagttg ttcaaaaatc tgttttgctt cgatcaaaaa gccaatagcc
    13681 agcaattctt cgcctagttc agctaatata tctgctgggt cttcttttaa tgctttttct
    13741 aaaaagattt gtgcttgtga caagtcttca tctgataaag cttgcaacat tttttcgcta
    13801 tttgtattca tttcaattcc ctcttatctc actgacgatt tgttgaaata ccgtcattgt
    13861 tctcaataat tctttttcgt ttggatgttg gtatccgtct attataccta ttttttctaa
    13921 aacaagtatt tttttgcttt tttctgccaa aaaaagaact ttttccaaat actccatctt
    13981 tgaaatctgt tccagccagt ggattggata gccaagcaag tccagccaca caaaaaagtt
    14041 ttttagttgg aaatcaaaag gatatgcttg gagattccac acgaaatgaa acatcattcc
    14101 taaaaatttt ttcatactag cagaaagtag atggccattt tcggtcagat aaaaagcttg
    14161 ttcaaacagt ttaccataat ttgacaggtt ttctgcctct aattgataaa aatagatcat
    14221 ttcttcgatc atcccggcaa acggccgagt cgttaattgc tttttatttg gatagttttt
    14281 atacagattt tgcaaaaaac gatgatcaca aatcattccg caacagataa aagtcaaaaa
    14341 gtcaatctgc tttccgtcca cctgccgctc gctgattgtt tgatcataaa cgatgcgttc
    14401 tggcagattg atggtttgca gcactacacg atttggtagc tgcaaaatcg tccgctgcgg
    14461 tgttaatgct cggcttagag aacggatcga tacagggatc atccatagtg atgaatcaag
    14521 ggttgtatgt ttttgaaaaa aacctgccag atccatgatc ccttcatttc caaatccgat
    14581 gataaacatg gattgatttt cgggatagcg tttgacgaaa tcaagcaggt tctttaattc
    14641 tgttaaatga ttgcattgag tgttggcaca aatataccag tcattgtttg cttgatttgc
    14701 aaataacggg ttgatcttat ctgcaaacaa atcataatag cgctgattcg ttaaaaataa
    14761 gattggctga tcaatggaaa gttctcttag ctgactgact agcgtttctc cgtaaaacac
    14821 tttcgtcaac gcatcttcta cttgataatc aagttccata cgcatccctc ctcttcttat
    14881 ttttatttta ccattagtaa gcaaattcta gaaaagataa gagcaaagct cacctcataa
    14941 cttgcaaata aataaaaatt aaataaaata catgtgtttt ctatgtatga catacggatt
    15001 cggaatctct gtctcttatt tattcctttt ctgctaggaa ataaaaagaa aagcacttgt
    15061 agccctagag ccacaagcgc tttttcaact gtcttattta acagcgtctt ttaatgcttt
    15121 acctggtttg aatgcaggta ctttgctagc tggaatttcg atttcttcac cagtttgtgg
    15181 gttacgacct ttgcgagctg cgcgttcgcg aacttcaaag ttaccaaaac cgattaattg
    15241 aactttttcg ccgttagcta aagcatcttg aatagttgaa aatacagcat ctactgctgc
    15301 agttgcatct tttttagtta aatcagtagc tgaagcaact ttttcgatta attctgcttt
    15361 gtttgccatg gatagattca cctcctccac aagagatatg atagaaatat catttagtta
    15421 aaactttctg agtggtccag aagtttaaaa agaaatattg attaacttca atattctgtt
    15481 atcacgatat catatgaacc gcttaatagc aaggttttta ccaattttca catgcttttt
    15541 ttagtcatag aaacgtaaca aacgaaactg tctttttctt ttgaaaaaaa aacgtcgata
    15601 tggtaaaatg cctattttct cctacgaggg ataattcgga tggacgtccc ttcaaaagta
    15661 aatgcttttc tgatttgatt ttctaagaaa cgttcataag aaaaatgcat caattcctct
    15721 tcattgacaa aaacgacaaa tgtaggtggt ttgacagcga cttgtgtagc ataaaaaatc
    15781 ttcaggcgtt ttcctttatc tgttggtgtt ggattgattg ctacagcatc cataatgata
    15841 tcattcaaca cagccgaaga gatacgaaga ttctggttca tacttacttg ttcgatcatt
    15901 tcaggcagac gttccaaccg ttgtttagtc actgcagaaa caaaaacgat tggagcgtaa
    15961 tcaagatacc ggaattcatc acgaatctct tgttcgaaat cacgcattgt attggtttct
    16021 ttctttacaa gatcccattt attcacgacg ataataattc cacgaccagc ttcatgggcg
    16081 taacccgcta ctcgcttgtc ttgttcccga atcccttctt cagcattcag taccatcaaa
    16141 acgatatcag aacggtcaat ggcacgcatt gcacgcatca cgctgtattt ttcagtattt
    16201 tcgtatactt ttccgcgttt acgcatacca gctgtatcta tcattaagaa tttctgacca
    16261 ctttcagaaa cgaagtgggt gtcgattgcg tctcgggtcg ttccttcaat atccgaaaca
    16321 attacacgtt cttctcctaa aatcgcattg atcaaagagg atttaccaac atttgggcga
    16381 ccaatcaaac taaacttaat cgtgtcgtcg tcctcttctt cagtctcttc agagaaatac
    16441 ttcactgctt catctaaaat atcaccaatc cctagtccat ggcttcctga aacaggataa
    16501 ggatcgccta gccctaagga ataaaattca tagatatcgt ttctcatttc tggattatct
    16561 actttattga ctgctaaaat cactggttta ttacttctgt ataaaatttt cgcaaccatc
    16621 tcatctgcgt ctgtcacgcc ttcgcgtccg ctgactacac aaatgattac atctgcttct
    16681 tcaatcgcga tttctgcttg atgcttgatc tgatccataa aaggttcatc gccaagatca
    16741 attccgcctg tgtcgatcac gctgaattct cgacctaacc attcaccttt tgcataaata
    16801 cgatcacgag ttactccagg agtatcttcg acaatcgaga tacgctcacc agcgatgcgg
    16861 ttaaaaatcg tagacttacc gacgttcggg cggccaacga ttgcaattgt tggatttgcc
    16921 atattatttc ctcctcacaa gtttccaatc tcacttagtt taacgaatgc attgccaact
    16981 ttcaagcata gattgtcaat taaacgaagt atttttattc cttattgaaa tactaatcgc
    17041 ttttcaaaag aaaaactgag acaaaagcag ctgctttcat ctcagtcaca gatacaaaat
    17101 caattatttt tcagaatcat cggttgcttt ttcttctact tcttctttca acgcatcacc
    17161 taaaatgtct cccatagtaa atccagtatt ttcttctggt aattcataag actcaacatt
    17221 ttttggttct tcttctgaag ctggtttttc ttctaaagct ttgatactca atgcaatacg
    17281 atgttcttct ggatggatct ctaatacttt cactttcact tcgtcacctt catgaagcac
    17341 ttcatgtggt gtcgcaatat gtttatgtga aatttgagag atatggacta gcccttctac
    17401 tccagggaac acttcaacaa aggcaccgaa gcttgtcaaa cgtttaactg ttccgtctaa
    17461 tactgtgcca actggagctt ttgtatcgat ttctgaccaa ggtccaggct gtgtgtcttt
    17521 gattgacaag gaaacacgtt cttgttcagg attgatagac aacactttga catttacatc
    17581 atcattgaca gccaatacat cacttggttt tgctacatga ctatgtgaaa tctctgagac
    17641 atggactaga ccgtcaattc cgcctaggtc gacaaatgcg ccaaaatcag taagacgtgc
    17701 aacacgtcca ctgagaacat ctccctcatg gatagttgaa agaatttctt ttttcttttc
    17761 ttctctttcg ttttccacta ccgctttatg cgataagatc aaacgatttt ctgatggttc
    17821 gatttcgatg attttaaagc tcaatgtttt gcctttatag tctgaaaagt ctgcaacaaa
    17881 atgatcttcc accattgatg cagggacaaa accacgtaca ccgacgtcaa cgactaaacc
    17941 gcctttgaca acatttgtaa caggtgcttc gatgaccttg ccctcttgga aatctctttc
    18001 gatatcttcc caaacttttt tagcatctaa acggcgtttt gaaagtagat agctgccgtt
    18061 ttctttatct ttgccgatcg tactgataac gactaaatct aatacatcac cgacttttac
    18121 tacttcgctg atatcttcta ccggcaaagt tgataattct tttgccggaa cgactccttc
    18181 aactccagcc ccttcgattc cgacaatcac ttgtttgtct tcaatgacta aaacttcgcc
    18241 cttaacaaca tcgccaactt ttacttcttg tacgcttttc attgcgtctt ccattgactg
    18301 gttgttctct tgattttgat caaattctgt cataacactt gtcctcctaa ccaacattct
    18361 gccgtaagta cggtactgtt tttagtttaa ttgattattc aaaaaaaata aagcgatttc
    18421 gttcgttttc cttgaaaaaa caattgtttt tcattttttt tcttaattta aaaaagaaga
    18481 taacct
//
LOCUS       DACIRY010000049        18318 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.050, whole genome shotgun sequence.
ACCESSION   DACIRY010000049 DACIRY010000000
VERSION     DACIRY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.050"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(48..725)
                     /gene="phoU"
                     /locus_tag="IYE22_001463"
     CDS             complement(48..725)
                     /gene="phoU"
                     /locus_tag="IYE22_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ9506679.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(738..1496)
                     /locus_tag="IYE22_001464"
     CDS             complement(738..1496)
                     /locus_tag="IYE22_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506680.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1512..2318)
                     /gene="pstB"
                     /locus_tag="IYE22_001465"
     CDS             complement(1512..2318)
                     /gene="pstB"
                     /locus_tag="IYE22_001465"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506681.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2333..3217)
                     /gene="pstA"
                     /locus_tag="IYE22_001466"
     CDS             complement(2333..3217)
                     /gene="pstA"
                     /locus_tag="IYE22_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ9506682.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3217..4137)
                     /gene="pstC"
                     /locus_tag="IYE22_001467"
     CDS             complement(3217..4137)
                     /gene="pstC"
                     /locus_tag="IYE22_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ9506683.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4252..5109)
                     /locus_tag="IYE22_001468"
     CDS             complement(4252..5109)
                     /locus_tag="IYE22_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ9506684.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5446..6894)
                     /gene="cls"
                     /locus_tag="IYE22_001469"
     CDS             complement(5446..6894)
                     /gene="cls"
                     /locus_tag="IYE22_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ9506685.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(7046..7939)
                     /locus_tag="IYE22_001470"
     CDS             complement(7046..7939)
                     /locus_tag="IYE22_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506686.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(7923..8609)
                     /gene="ftsE"
                     /locus_tag="IYE22_001471"
     CDS             complement(7923..8609)
                     /gene="ftsE"
                     /locus_tag="IYE22_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ9506687.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(8714..9815)
                     /gene="prfB"
                     /locus_tag="IYE22_001472"
     CDS             complement(join(8714..9751,9753..9815))
                     /gene="prfB"
                     /locus_tag="IYE22_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ9506688.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(9941..12475)
                     /gene="secA"
                     /locus_tag="IYE22_001473"
     CDS             complement(9941..12475)
                     /gene="secA"
                     /locus_tag="IYE22_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ9506689.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(12697..13248)
                     /gene="raiA"
                     /locus_tag="IYE22_001474"
     CDS             complement(12697..13248)
                     /gene="raiA"
                     /locus_tag="IYE22_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ9506690.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(13376..14095)
                     /locus_tag="IYE22_001475"
     CDS             complement(13376..14095)
                     /locus_tag="IYE22_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ9506691.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            complement(14092..15405)
                     /locus_tag="IYE22_001476"
     CDS             complement(14092..15405)
                     /locus_tag="IYE22_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ9506692.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            complement(15486..15806)
                     /locus_tag="IYE22_001477"
     CDS             complement(15486..15806)
                     /locus_tag="IYE22_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9506693.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(15914..16201)
                     /locus_tag="IYE22_001478"
     CDS             complement(15914..16201)
                     /locus_tag="IYE22_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506694.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            16327..16971
                     /locus_tag="IYE22_001479"
     CDS             16327..16971
                     /locus_tag="IYE22_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ9506695.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            complement(17030..17737)
                     /locus_tag="IYE22_001480"
     CDS             complement(17030..17737)
                     /locus_tag="IYE22_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9506696.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         5119 a         4004 c         3199 g         5996 t
ORIGIN      
        1 tttcacaacc tcttttccta aacaaacggc agaatcaccg ctttagttta gaaaatctca
       61 ttgtggttcg tatttaactc agtgattttc cctgtagcca agtagacgat ccattcacag
      121 atgtttgtca catagtctcc gatccgttct agataactgg caacattcaa gtagtctgtt
      181 ccactgatga ctgtctcagg attttcctgc atatttttga ttgttgcatt atagattcga
      241 ttgaaatagt cattgactct ttgatccata ttggcgatcg tacgtgcgtc tttttcgtct
      301 gttttgacat aagcaactag gacgttgtcc accatttttt tcacataatc ggacatatcg
      361 gagatctctt tttcgatttc cggtattctc gtctgccctt ttacgcgaat cgtggattta
      421 gcaacagaca ccgcatgatc ggccatacgt tctaaatccg agctagcttt catgaccgta
      481 atgatcatac gcaaatcagt tgtaactggc tgttgcaatg cgatcatttc aaaacttttc
      541 ttctccaagc gtgtttccat gttattgata tttatatcgt tttcgataac ttcttgggct
      601 aagattttat cgtgtttgat gtatgcacga actgacttgt gcacggcgct gctcaccatc
      661 atccccattt cgtagaactg attatggaga ttcagcaatt cttcttcaaa ttgtgtacgt
      721 agcattccgt ttcctcctta tccaaatcga cctgaaatat aatcttctgt ctcttgtttt
      781 tctggattca tgaatatctt cttcgtatca ttgtattcga tcaagtgacc atctaaaaag
      841 aatgccgtct tatctgaaat acgtgaagct tgagacatat tatgggtcac gatgatcatc
      901 gtataccgct ctcttaattc taacagcata ctttcgattt tcccagtaga aacaggatca
      961 agcgcacttg ttggttcatc caataagatg atttccggat taactgccaa aacacgtgcg
     1021 atacatacac gctgctgctg cccaccggat aaagataaag cacttttatg caatttgtct
     1081 ttcacatcat cccaaacaga cgctgccttc aggctttctt caactacttg atcaaggact
     1141 tttttatttt tttccccttt taatttcaat ccatagatga cattttcata gatagaaaaa
     1201 ggaaatgggt ttggctgctg gaataccatg ccgatttctt ttcttagttc aacaacatct
     1261 gtttttggac catagatgtc tttgtcttta tataaaacac tcccggtgat cgttacattc
     1321 ggaatcaggt cattcatccg gttcaaagaa cgcagataag tggatttccc gcaaccagaa
     1381 ggaccgatca tcgcagtgat ttccccttga ttgaaactta gatcgatccc ttttagtgct
     1441 tctttctttc cataatacag atgaagatct ttagatgaaa taatttcttt tgtcatgttg
     1501 aggcctccgt cttaaccaaa atgaccggac acataatctt cggttgcctg aatttttggt
     1561 ctggtaaata tcttgttagt tttgtcatat tccattactt ttcctagata gaaaaaggca
     1621 gtatagtcgc tgatacgagc cgcttgttgc atgttatgcg tcacgatgat gatcgtatag
     1681 tcttctttca agtttacaag tgtttcttca accgttcctg tcgaaatagg gtccaaagca
     1741 cttgccggct cgtctagcag caagatatca ggtttcatcg cgatcgcacg agcaatacat
     1801 aaacgttgct gttgcccgcc agataaagcc aaggcacttt tgtccaagct gtcttttact
     1861 tggtcccaca aggcagcttg tttcaagctt gtttcaacga tttcatctaa tttctttttg
     1921 tttttttcgc cgtgctgttt caaagcaaat gtaatattgt cataaatcga cttgctgaac
     1981 ggattaggac gttgaaaaac catgccaatc cgtttgcgca tctcataaac atccacttga
     2041 ggcgcattaa tatctacgcc ttgatacatg attttcccag tgattttcgt attagcgatc
     2101 ccatcattca ttcgattcaa cgaacgaaga tacgtagatt ttccgcagcc ggaaggacca
     2161 atcagtgcag tgatcttatt tttttcaaat tccagatcca ctcctttgat tgcttcattg
     2221 tcgccatacc agacatgtag atcatccgta tatagtgcta cttctttctc ttttggcatg
     2281 gtgatgacat ttgtttcatt ccagttatat tttttcattt tgcttcttct ccttaggctg
     2341 aagtcatttt tcggtataat cgtttgccaa tcgcacgagc gccgaagttg aacagtaaaa
     2401 cagcaatgat caacacggca gaagcgccag ccgaaacaga aacaccatca ggcatcgtgc
     2461 cttctgtatt gatcttccaa atatggacag ctaatgtttc agcttgacgg aaaatgctga
     2521 tcgggcttga aacacttaat ggattccagt tcgtaaaatc taacgcaggt gcactttgtc
     2581 cagccgtata gatcaatgca gctgcttcac caaaaattct tccagaactt aggatcacac
     2641 cagtcaaaat ccctggtgtc gcttctgaaa ccaccacttt tatcactgtt tcccagcgtg
     2701 acaaacctaa agccaatcct gcttctcgtt gtgtgtaatg gaccgcttgt aaagactcct
     2761 caactgttct agtcaaaaga ggcaagttga atacagtcaa agccaatgca ccagaaataa
     2821 tcgagaaacc atactggaat tgaataacga aaaccaagaa accgaataaa ccgacaacga
     2881 cagaaggcaa tgagctcaag atttcaatcg acgtgcgaat cacatctgtc agccagtttt
     2941 ttccagcata ttctgaaaga tagatacccg cacctaaaga aataggaaaa ctgatgatca
     3001 tcgtgatcaa aagcaaataa atcgagttaa atagctggat cccgattcca ccgccttctt
     3061 ggtaggcttt ggaaggttcg gtcaagaagg accatgaaat gtgcggtatt ccccgaacta
     3121 agatataaag tagtaaagct gctaaaatca atacgatgat ccccgaaact acatataaaa
     3181 cacctgtagc aattttatct gagcgttttg cgttcattat ttcagctctc ctttcttccc
     3241 aatcatacgg atcacgatat taaagaataa tgacatcaat agcaagatca gcgctaatga
     3301 ccataacaca ttattttcaa ctgttcccat gattgtattt ccgattccca ttgttaagat
     3361 ggacgtcaat gtagaagcag gagtagtaag tcctgttggc atcaatgctg cattaccgat
     3421 gaccatttgg atagctaagg cttcaccaaa cgcacgagcc ataccaaaaa cgactgctgt
     3481 taaaatcccg ggcacggctg ctcgtagcac gactttgtag attgtttgcc atcttgtggc
     3541 accaagcgct aatgaagctt cccgatagtg ccgtggtact gcctttaatg catcaacagt
     3601 catcgttgtg acagtcggca agatcataac gaacaacaca aatgtaccag ctaagatacc
     3661 aaaccctgtc ccgccaaaaa tacttctgac aaaaggcacg ataactgata aaccgataaa
     3721 accataaacg acagaaggaa tacctacaag cagctcgatg accggctgta agatcttcgt
     3781 tccgttttta ggagagatct ccgtcatgaa tactgctgca ccaatggcaa atggtgtggc
     3841 aacaatagca gataaaaagg tcacaataaa cgaacctgtg atcataggca atgcccctac
     3901 cattggtttc ccatttgctc cgatttggct tggattccaa tctgtaccaa acagaaaatc
     3961 aaatacattg atcttatcag taaaaaaggt ggccaatcct ttactagcta caaaataaaa
     4021 aatagataat acaaccaaca cgatcagcgc gatacataaa aaactgatga attttccacg
     4081 ctgttccatt tttgaacgtt ttgattttgt taataatact tttttcacat cttccacaga
     4141 tgactccccc ttaatcaggt ttaacatact cactcagtgt acaaattgaa catcaatttg
     4201 gttttaataa catgtaaaag tttagctaag tttgtaaatt ttgtgtaaag attactcttt
     4261 aacgatattt ccttgccaat cacgttcgac tttcatatga gaaacaggga tataacctag
     4321 ttctccaacg atgttatctt gtacttcatc agataagata tagtctaaaa attcttttgt
     4381 cagttttttt ggatgatctc ttgtgtacat atgctcatat gcccaaatcg tccatttgtt
     4441 cgttgtcaca ttttcatctg tcggttctac accgttaaca ttcaacgtat ctacatcatc
     4501 ggaaacataa gaaaaagcaa catagctgat tgcaccgggt gtgtcggcaa cgatctggcg
     4561 caccattccg cttgaatctt gttcttgtgc ctgaacagct gtttctccgt ccaataccca
     4621 tttttcaaac gtgctacggg ttccgcttcc tgaagcacgg ttcaatataa ctactggctg
     4681 atcttttcca ccgacttcac tccagttttt gatctcgcct gtaaatagtt ttttcaggtt
     4741 ttccatactg atgtctttta cgccaatatc cttgttcaca ataggagtga tcccaacaac
     4801 tgctactcga tgatcgacta atgctgaggc atcgatcccc gatttttctt ccgcaaacaa
     4861 atcagagttt ccgatgtcga ctgctcctga ctgtacttga gacaaaccag ttccgcttcc
     4921 gcccccttgt acattgatga attttccagg attttctgtc tggtaagttt cagcaactgt
     4981 ttctactaaa ggctgtaaag cagaagagcc aacagccgta atggattctc ctcgatcaat
     5041 ccactttgaa cagcctgata aaagaatagc taatcctatt attggtaaga ataatttaag
     5101 ttttttcatt agaaatctcc tttaatccta aagtatgttt attttatcat tttcattact
     5161 tgatgaaaac gaatcaaaat cattaaataa caaaataaat cctcataatt gtaaaaaaaa
     5221 ctatctcatt aaaagtttct atgctatact ggctttgaaa tgtgtctctt ttaatagcat
     5281 atattagctt atttcttgct ttccccaaaa aacaagagat accccaaagt taatttcgct
     5341 catgcgagac gctttctcca tttctgacgt actctcagaa atggccccgt tagaaaagga
     5401 cacgagatct ccatctgttg atggttctca tgtccttttt gtgttttaaa ggagcggcga
     5461 aatcaatctt gctaatcctt ctttcatctt gatgattatt cctcggtttt catacatttc
     5521 ttttgtgaga tgcgtagaaa ttttcgagtc ttcaaaaaat gcttttctta cacgtgaagc
     5581 cattcgttca tcataaatca gtgtattgac ctcaaagtcc aagcgaaatg aacgaacatc
     5641 gatatttgct gagccaactg aaacaatgcc accatcgata atcatcgtct ttgcatgaat
     5701 aaatccattc tcatatgttt cgatccttgc tccatactcg atcagctctg cagcgaaaga
     5761 atacgttgcc cagtaaacga gaagatgatc tggtttgtta ggtatttgga tatgaacttt
     5821 tacgcctgaa agaagagcca acttcagtgc ttcatgaatc gatccatccg gtatgtaata
     5881 aggcgtttgg atcaatatct cccgtttggc taacgaaatc attttcaaat aagtcatttt
     5941 gatttgttca tgttcggtat ctggtccgct tgtcacgatt tgaacagcta tatcaccagt
     6001 agagtgaatg gacggaaaaa atttgggaga atagcctact tcttttgtat gctgagaatt
     6061 ccagtccatc aaaaaacggt tttgcaagat atacaccgct tctccgtaaa tacgcaaatg
     6121 attgtctcgc caataaccaa atttctttac caatcctaga tattcatttc caacattgaa
     6181 cccacctgta tatccgattt tcccatcaat aactacgatt ttacgatgat tgcgataatt
     6241 gatccgagga ttaatatacg gaacaaatag aggaaagaaa aatgcgattt ctccgcctgc
     6301 ttttttcaaa ttctgaaaaa agctggaaga aacttgtgtg gaaccccatg catcaagaag
     6361 gactctgact tttacccctc gtcttgccgc ctctgtcaat gcatctcgaa cttcttcacc
     6421 taatgcatca ctgcgataaa tataatactg gaagtggata tggtcttctg cttgcttgat
     6481 atcttcaatc agcgcatcga atttttcacg accatctgta aataaagtga tttcattttt
     6541 agtcgtatag agcgtacttt caaataccgt gagcatatag atcaattgtt tcacatctac
     6601 ttgtccagta ggcggatgag gataaagccc tcgcatcaat gcctgtttgt cttcttcaat
     6661 ttccaggttc ttaccgattt tcccttggat cttcaaatca aagattttct ctttggaaat
     6721 ccctcttcct aaaaaaatat ataaaataaa tcctacaact ggaatgaaca tcaaaacgag
     6781 cagccatgcc catgtttgag cagtttcctt tctttctcga aaaacgatga taaaggaaag
     6841 caaaatattc ataatcaaaa ggatcgtaaa aaaattgtca acgataattt tcacggcttc
     6901 acctcttcct ctaccaactt aagaataccc aagttgtttt ttaaagtaaa gggaaaaaag
     6961 ccgcttcctt ctattctcct agccaataaa aaaagaaagc tggaacgaaa ctgtccaact
     7021 ttctcatttt atttatattg tggtattaga atttcaagaa acgtctcata gaaatgatcg
     7081 agccaagtga accaatgatc acaccaatgc cgaccattaa aagattgatt ttccaaacga
     7141 tattttgtgg atgaatcaat gaataatggg aacgtaataa tgatggattg atcaattgga
     7201 aaaaccatgc ataaccaaat gtgatgatca aaatcggaag aatcgcaccg atcagaccga
     7261 tccagccacc ttcaaggaaa aacggccagc gaatatatcc attttttgca ccgactaaac
     7321 gcatgatttg aatttcacgc tgtctagaca aaatcgtgat ccgaatcgtg ttcgaaatga
     7381 gaaacatcgc tacgaacagc agcaatgccg ctgcaactaa tccccatgtc cgaactgcac
     7441 cagcgatttg gaaaatccga tctgaggaag ctccaccgta atcagaacga gaaacattct
     7501 tcaaatctgc tgcttcttct gctacttgtt ttgtataaga aggatcagta gcacttacta
     7561 catacacatc atataacggg ttattgtcac cttcaaacaa tttccaggaa tcacccattt
     7621 cgttttggat ttttttcaat tcctgttctt tacttgaaaa ttcaactttt ttaacatgat
     7681 ctaagtcatt caattctttt tctagctttt tcatctcttc gtcttttgta ccgatatcta
     7741 cgaatacaga tacatcaacg tttccttcta tatcctgagc taatttagtt gcattcatga
     7801 taactgccat gaatacaccg actaaagtca acgtgatcgt taccgcactg actgaagcaa
     7861 ctgtcatcca gccgttacgt ttcaaactct tgatactttc taataaatgg cggaaaaagg
     7921 ttctaatcat cgtatccata ttccccttcc gcttgatctc gaataatgcg gccgttttcg
     7981 atagcaatta ctcggtgccg aatcgtattt acgatcgtac tgttatgtgt agccatcacg
     8041 acagtagttc cttgggcatt gatccgatct aataatttca tgatttccca tgagttttct
     8101 ggatccagat ttcctgtcgg ttcatccgcg ataagtactt taggcgtatt cacgattgcc
     8161 cgagcaatag agacacgttg ctgttcccca ccggataatt cgctaggaaa gactcgaact
     8221 ttgtgtttca aaccaacaag gtccagcact tccattactc gttttttgat atctcgaggt
     8281 tttctgccga taacttgcat agcgtacgca acattttcat atactgtttt tcttggtaat
     8341 aatttatagt cttggaacac cacgccgatt tctctgcgta gataaggaac ttcgcggttt
     8401 ttgattgcta ttagatcatg acctgcgacg ttcaaagttc cttttgaagc tttttcttct
     8461 cgatacatca atttgatgaa tgtagattta ccggctccag acggaccaac cacatagaca
     8521 aactctcctt gttcgatgac aatcgagaga ttgcggatag cggtcgtacc atttgagtac
     8581 ttcttcatta catccttcat ttcaatcatg gcgttctctc catatctttt tcagtcttta
     8641 gctattatag catggcaatg tttcaacatg cttgcaaaat cttacaattt tatttcaatt
     8701 taacaatggg ttcttattgt agatgcattt ttaaatagcc gtcaataaat aaatcaaggt
     8761 caccgtccat aactgcttgg acatttcctg tctctacgtt tgtccgatgg tcttttacca
     8821 tagaataggg atggaaaaca taagaacgga tctgtgaacc ccagccgatt tctttttgtt
     8881 ctcctcgaag tgcagctgct tcttgttctt tcttttccac ttctaattga taaagcttag
     8941 cttttaacat ccccatcgct tgctcacggt ttttcaattg tgagcgttgt gcctgactag
     9001 ctactactgt tcctgtaggc aagtgagtga tccgtacagc agattctgtt ttattgatgt
     9061 gctgaccacc tgcaccactt gctcggtacg tatcgatttt caaatcatct ggattgattt
     9121 ctatatcgat cgtttcatca agctctggca tcacatctac cgaacaaaaa gaagtatgac
     9181 gtcgtttcgc tgagtcaaaa ggcgaaatcc gtacaaggcg atgaacacct ttttctgatt
     9241 ttagataacc aaaagcgtta tgtcctttaa tcagcagggt cacactcttg atccctgctt
     9301 catcacctgc ttgataatct agtgtttcta cttgatagcc atgctgctct gcccaacgtg
     9361 tatacatacg taaaagcatg ctgccccagt cttgggactc cgttccgcca gcccctggat
     9421 gcaattcgac gatcgcattg tttcgatcat aaggtccgtc taacagcata gataattcat
     9481 acgtcttcat tttttcatct aatgcattga tacgtgtttc tgcttcttct tgcattgctg
     9541 catcaggttc ttcttgcagc atctccacca tcaattcgat ttcttctagt tcttcggcaa
     9601 gagaatggaa ttggtcatag gtttctttat tagcgttatt ttcactgatc actttttgtg
     9661 cggcttcgct gtcatcccaa aaaccaggtt cagccatttt tgcttctgct tccgcgatgt
     9721 cttcttccag ctgatctaag tcaaagagac ctcctgaaac ttgtgacctt ttcctgcatt
     9781 tctgtcagga gatttcgaat ttcggatatt tccatgtttt ttccttcttt cactatactt
     9841 ttttcattat atctagtcta acagaatcag caaataacag aaagaaggtg agacgaattc
     9901 ttaaaaaaag aatttgtctc accaatcctg ctgtcagaaa ttagtttgca ttttttccat
     9961 gacagttctt atattttttc ccgcttccac aaggacaagg atcatttctt ccgaccttgt
    10021 tcacatggat tggctgtttt ttcgcgctag tattactatt ttctttggca tctccgtctt
    10081 ctgagtgaga agctgttcct tgagaaacct gttcacgctg tacgttttga cggatctcag
    10141 ctttcataaa gattcgagtg acttcatact cgatggcgcc taccatatct tcaaacatct
    10201 tatagccttc tgtttgatat tcgactaatg gattgttctg tccataagca cgtaagccga
    10261 ttgactgacg taattgatcc attgcgtcaa tatggtctgt ccatttagag tccacaacac
    10321 gcaagatcac gactttttca aattccaaca gttgctctgg accatttagt tgagccgctt
    10381 tggtattaaa tacttcttgt gctcgttcgt ttagatactc tttgatctcc tgagctgttt
    10441 ttccttcgat atctgcaaga gtaatactat cttcatggac aagagtactg ccggcaaaat
    10501 cgatgatact ttccaaattc caattttctt tctctaattg tgtatgcgca tctactacac
    10561 gactgattgt ccgtttgacc atgttcatta gtggcttgga taagcttttt tcttccatga
    10621 ttacttcttg tcgctgttta tagattactt cacgttgttc acgcatcaca tcatcgtatt
    10681 gtaagacgtt tttacgtgtg tcatagttat ttccttctac acgtttttgt gcagattcta
    10741 cttgtttcgt cagcatcttg ctttggataa cagcatcttc ctcaccgatc ttcatccgat
    10801 ctaagaaagc tttgattcgt tctgaaccga atcgtttcat caaatcatct tccaatgaca
    10861 agtagaactg tgacatacca gggtctcctt gacgtcctgc acgtcctcga agctggttat
    10921 cgatacgacg agattcatgt cgttctgtcc cgataactgc tagaccacca agttctctta
    10981 ccccaagtcc taacttgata tctgttcccc gaccagccat gttggtagca atcgtcacag
    11041 cacctttttg accagcattc atgatgatct ctgcttcttt aaagtgattt ttcgcattca
    11101 gcacttcatg aggaatacgt tcttgattca gcatatctga taataactca gatgtttcaa
    11161 ccgcaacagt tccgactaac acaggttgac ctttgcggta gcgatctttg atatcttgca
    11221 cgacagcgtg gaacttgctt tgtaatgttg ggtaaagcaa atctggacga tcatcacgga
    11281 taaccggacg atttgtcgga atctggtata cttggatgtt ataaatttct ctgaattctt
    11341 cttcttctgt tttagctgta ccagtcattc ccgcaagttt cttgtacata cggaaatagt
    11401 tctggaaagt gatcgtcgcc atggttttcg tttcgtcttc gatctctacg ccttctttag
    11461 cttcgattgc ttggtgaagt ccatctgaat aacggcgtcc atccatgatc cgaccagtga
    11521 actggtcaac aatcagtact tttccgtctt ggacaacata atcgatatct aaaagcatga
    11581 tatagttggc tctcaatgct tgatccaagt gatgggtcaa tgcagtattt tcaatgtcgt
    11641 ataagttatc caaaccgaat gtttcttctg ctttttcgat ccctgcttcg gttaaactga
    11701 ttgtttttga ttgtacatcg attttataat cttcttcttc tttcaaacgt ttcacgaagt
    11761 tgtctgctct tgtatataaa gctgtggatt tttctgcttg ccctgaaatg atcagcggcg
    11821 ttctcgcttc atcaatcaag attgagtcaa cttcatcgac aatggcataa ttcagcggac
    11881 gttgtaccat ttgatggcgg taaacgacca tattgtctct caagtagtca aatcctaatt
    11941 cattattcgt actataagtg atatcacaat tataagcctc gcgtttttca tccgaagatt
    12001 ttgagttgat attcaacccc actgttaatc ctaagaaatt ataaagttcg cccatttccg
    12061 tcgagtcacg ggttgctaaa tattcattga cggtaactac gtgtacccct tcgccagtca
    12121 gagcatttag atatactggc atcgttgcgg tcaaggtttt accttcacca gtacgcattt
    12181 cagggatatt tccatcgtga agcacgattc cacccatcaa ttgtacgtgg tatggatata
    12241 aacctaatac acgttttgct gcttctcgga cgactgcaaa agcttccgga agtaattgat
    12301 ctaatgtttc tcctttttgg tatctgccct tgaattcagc agtttttgcc tgtaattgtt
    12361 catcagttaa tgctgccatt tggtcagcgt atgcttcaac tttttttgca atgctatcta
    12421 aacgttttag ttctttctta tcgttttcga taagtgaacg aatcaaattg gccatgcgtt
    12481 gtgtaatctc cttttccttc tgtgtattat ccagactatg tagactttac ttttcatttt
    12541 atcattagtt atagataatt gaaatatctt aagcgtatta aagtaagaac ttcataattt
    12601 attcatattt catctaaaag aggaggaaac tcaaaaaaac ttgcggcagc tgtcagtagc
    12661 tgctgcaagt tttcgttgtt atttaagtaa ggctgatcag tctgtttcaa tcagtccgta
    12721 ttttccatct ttacggcggt aaacgatact tgtaccattt gtttctgcat cttcaaaaat
    12781 aaagaagtta tgtcccagca tgttcatttg caagactgct tcttcactat ccattggttt
    12841 caatgaaagg cgtttcgtac gtacgatatc taattcagaa tcattttcgt tttcgtcctc
    12901 ttcattgaat aaagcagcag catttgcagt attcatgccc gtttcacggg atttacgatt
    12961 aatttttgtt ttgaatttgc ggatttggcg ttccaatttg tcaacaacca aatcgatact
    13021 tgcgtataaa tctggagaag tttcttcggc acgtaataca agataaggta atgggatcgt
    13081 tacttccact tttgctgttt tttctgtata aacttttaag ttcacatgcg ccgttgcatc
    13141 gggtgcgtca ctgaaatagc gttctagttt gcctactttt ttctcaacgt agtcgcgaat
    13201 cgcttcagtt acttcgatat tttcgccgcg tacattatat ctaaacataa aaattacccc
    13261 tttcgtctac cacaatcaag aaggagaagg accactcttt ccctcttctt aattctatta
    13321 tatcgaactc taggttattt gcaaattaaa tcgctatcaa atttccacat ctaattcatc
    13381 tcgccaaaga aatagttcgg atgattttcg gctgtgcgat ctgcaaacaa tctgctgcgt
    13441 ggaaaagagt ctgtcctgtt gtatagacat catcaataat caatattttt ttattttgta
    13501 tcttagaaaa ggtgactgca agttcaaaag gttgaggcat ctccaatctt tcctttcgcg
    13561 tgttttttgc ttgagccagc agatgcttct ctcttttgag tagcatctga aaaggaattc
    13621 ttgctgcttt caacatctcg cttacttgat tgaatcctct ttctgttcgc cttttttcag
    13681 ataaaggtat tggacaatag atgtatgtcg ggtattcttt ttttatctgc gtccatttat
    13741 atgcaaaggt gcctgctagt cggatatctc ccataaactt gtattgattg agccaggcct
    13801 tcatattttc atcgtacaca aaaaaggcac ggtgctgaaa atcataggtc ggatatttct
    13861 tcttccattc ttgacagtca ctgcagtaaa cggggataga atttactttc tttttcgtac
    13921 tctctagatt gaaatggatt ggtttttgac agccagaaca gcctcttgtt tccaataaat
    13981 gaaattgtgc gcgacaaact gaacaagtct gaggtaatct gtatttcgaa acaccaataa
    14041 tctcgctgaa tgttaaattt tggacgatat ctttactgca gcaactgcac ctcacagtat
    14101 cttcttccct tcttcattca tttctttgat ttcctgacaa gcttttcgaa tagctgcagt
    14161 tttttcttca tagaaaaaat atacttgact gttcgtataa ggccctttac gatcggctct
    14221 tcctgcgatt tgtaccaaag aagatttagt aaaaaccgga tgatccgctc ctaaaacgat
    14281 aacggatatt ctttcaaatg tgactccacg ttcaaggatc atcgtggtta acagtatgtc
    14341 atattttgct tctcgcattt tctggacttt ttccgcccgc tgattgtctc ttgaagaaac
    14401 atctgtgatc tcatatctcg gaagagcctc ttgaagtgtg gctttcaaac gatgcatcat
    14461 cgtgatactt ggacaaaata aaaggatatg gttttcttta attaattgat gaatgatccg
    14521 caccaaagat tttatcttct tcttgttctt gcatttttgt cgccaattat tcatgaaaac
    14581 caagacgggt tcaggaagca aacgccgatg aaagcgtaaa gcaagtttgt ggatgtcaaa
    14641 cgtctgcttg atctccttta gtaacttttt atcaggtgtg gcacttaagt agatgaatcg
    14701 tccgtctggt ttaatcgctg tttgcacagc atgagctaat cctgcatccc cgttataagg
    14761 aaacgcatcg atctcatcga tcactattaa atcaaaggac cgataaaaac ggtatagctg
    14821 atgaatcgta cagataacaa aagaagtaaa ggtataagcc gttgctgtat tcccatggag
    14881 caacgtgatt ttttcttctg gaaaaacata acgatagcgt aaaaacaatt cattacaaac
    14941 atcgactcgc ggtgtacaaa tcgctactct tcctccagct tttaaagttt ccactaaaat
    15001 aggataaagc atctctgttt tccctgcacc tgttacagcc caaataagat gatgtctttt
    15061 ttctgaatgg caaagtattc tctttgagat ttccgtttgt ttttcagtaa gctgtcctgt
    15121 ccaaaaaaag acgacttctc gaggttttgc ttcaggctga tcaaacaaat ataaatcttg
    15181 ttgagtgtcg catcgcccta aatatagaca atgtgtacaa aaataaccgt aatctgcaag
    15241 ctgtccatca atgacggacg ttctcattcc acagcgatga caaagaatcc agttgctcaa
    15301 acgttccact gctgcgatct tctcataatt tggtaaattt acctcctcac agtctattgc
    15361 tttaatatct ctcgtcagaa gccttctacc ccaaaaatga ttcatattat tcgccctaaa
    15421 aaaatatacg aaaaaacaag cgatctattt tttggatcgc ttgttcgata gttatactgt
    15481 tctatttatt cacctatgag cgtgattgct tgatctaaca ctttttcgcc attcatcata
    15541 ccataatcag ccatattgat gacatctaaa ggaatgcctt ttggttccag acgtttctca
    15601 aaatcagctt tcataaagcg aacttgtggg cctaataaca acacatccac gtctttattt
    15661 gcaagattgt tatctgcgtc agaagcggac acagcgaaaa tatcagagtc gattcctttt
    15721 gcttcagcag ctttttgcat ttttgttact agtaaacttg tgctcatacc agccgaacat
    15781 acaagcatga ttgttttttt agccatactt ttctctcctt tttaaaacaa actacgcaaa
    15841 cgttttcttc gcgtctgtta ctctattata taaaaagaaa gaaatttgtc aacgttttct
    15901 aaacgcggtt tatttaatca aatagccaac tagatttcac taatttattg aacacgacat
    15961 attctttgtc caaattgatg acaactactt catagcctgc atttatccag ccataggcac
    16021 ctttttcagg gtgtttataa tcatttgccc accaatctgt actttccctt gctgttttgg
    16081 gaagtccgtt actgggaaac agcagttcgt caaattgggt aaaggtcaac gttacttgag
    16141 agccgccatt gttcttcaaa taatgcgtaa ttgcatcgta ctttttcatt cgttcctgca
    16201 tccccagatc cacctgcctt tgtctttatt atcgcatact acagcaattg cgcaaccgtt
    16261 acagtgttga aacaacctag aactttattt atactgaata aagaaaatga aaaaagaggt
    16321 gacaagatga tcgagcgcta tcgtacaata aaagaaaatg gacaaagtga gatcgagatc
    16381 aaaaaatcac gctttatctg ttctcttaga cgaatagaat cagaggacga agcaaaaagt
    16441 ttcattcaag aaataaaaaa agaacattgg aaagcgaacc ataactgcag cgccttcatc
    16501 attggagaaa aaaatgaaat ccagcgaagc agcgatgatg gagaaccaag tggaacggcg
    16561 ggcataccga tgttggaagt cctcaaaaaa aatcaactga tcaatgtagc tgcagtagtc
    16621 actcgatatt ttggaggaac gaaactcggc acaggcggat taatacgagc atacactcat
    16681 gcggtctctc aagcattgtc tgacattgga atcgtagaag gtaccttgca acaggagatc
    16741 tctgtgatga tcacgtatcc gcaactagga aaattacaga attatcttga actccaacat
    16801 ttttttataa aagaaatcca atatactgat caagttagcg tcatctgtgc tgtagatgaa
    16861 aaaagtgctg accactttaa agaatccatc attgagctgc tgaacggaca agtccaattt
    16921 gaagagggtc ctgtttctta tcacgagcaa ctggtaactg atcatatata aaaacaggtg
    16981 attctgattc tcttttattt ctaatacgga atcagggatc acctgttttt tatttttcca
    17041 aataaaactc aaaacgattt cctacgtatt gcgtacgcac atactcaaac ggcgtaccgt
    17101 cttcaaaata agaaatctgg cgcaaacgaa gaactgcatc tcctcgtttt acctctaaat
    17161 agtcagcaat tttctcagaa gccaacacag cagtaatcgt ttgattcgct ggaccgatct
    17221 ttttcccgcc tttttcttct agcgtgcgat acaacgattc agtaatctct gctttgctgt
    17281 agccatcgat cagcttttgt ggcacactgg caacttcaaa acaaataggg acatcatctg
    17341 cgaaacgaat acgttccatt cgcaagatcg tgtcatcttt actcaagttt agtttttcca
    17401 tttcacttga gcttggcgaa gtcaagaaat aagagattgt ccgactagac ggtactcgat
    17461 tttgtgattc cataatttct gtaaaacttg ttgcaccaga cattttttct tgtacttttt
    17521 ctcgtgcaac atatgtaccc gaaccgatct ttctttctaa gattccttca tctgccaagt
    17581 tttgaattgc ttgtctcaac gtcatacggc tgacattgaa tttcactgct aattctcttt
    17641 ctgaaggaag acggtctccg actttccata taccttgttc gatttcattt tttaattggt
    17701 catgaatctg tatatataca ggtaattgat tcgacataac gtacacccgt cctttcgttt
    17761 cttctattat aactggtata tacctgattc acaactgaaa aagataaagg tttctatcca
    17821 tcaacagagg atttagacac acatcgttct tcctactcct attgtcagaa acaatttttt
    17881 agatatgtcc aacctagaaa ataacaaaag accctaaaat cgatgaaaat gccgacttat
    17941 ggatcttttg ttattattag cggtttttag ccatccattt ttttgtatga ttcgttgggc
    18001 gttttgattt tacccggacc gcttttccgc cttttttcgg aatattcact tcaatattgt
    18061 atttttctga tttgaccaaa tcatataact tttcagctgc ttcgtctact ttttctttcc
    18121 atttcatgtg gatccctcca attcttgtag ttaacttaac tctatcttaa atcagcagaa
    18181 tcttcaagga acaaggtagc aaagcaaaaa aaagagctct cgaactaacg agagcttacg
    18241 attgtggtat actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg
    18301 ccttaccgct tggctata
//
LOCUS       DACIRY010000050        17740 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.051, whole genome shotgun sequence.
ACCESSION   DACIRY010000050 DACIRY010000000
VERSION     DACIRY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.051"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1030)
                     /locus_tag="IYE22_001481"
     CDS             complement(<1..1030)
                     /locus_tag="IYE22_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9506697.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IYE22_001482"
     CDS             complement(1112..1801)
                     /locus_tag="IYE22_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ9506698.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IYE22_001483"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IYE22_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ9506699.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IYE22_001484"
     CDS             complement(2328..2831)
                     /locus_tag="IYE22_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ9506700.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IYE22_001485"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IYE22_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ9506701.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IYE22_001486"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IYE22_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ9506702.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IYE22_001487"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IYE22_001487"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ9506703.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IYE22_001488"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IYE22_001488"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ9506704.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IYE22_001489"
     CDS             complement(6443..7462)
                     /locus_tag="IYE22_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ9506705.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IYE22_001490"
     CDS             complement(7635..8522)
                     /locus_tag="IYE22_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ9506706.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IYE22_001491"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IYE22_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ9506707.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IYE22_001492"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IYE22_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ9506708.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IYE22_001493"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IYE22_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ9506709.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IYE22_001494"
     CDS             9753..10397
                     /locus_tag="IYE22_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ9506710.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IYE22_001495"
     CDS             complement(10453..10956)
                     /locus_tag="IYE22_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ9506711.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IYE22_001496"
     CDS             11553..12518
                     /locus_tag="IYE22_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ9506712.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IYE22_001497"
     CDS             complement(12617..13789)
                     /locus_tag="IYE22_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506713.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IYE22_001498"
     CDS             complement(13776..14921)
                     /locus_tag="IYE22_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506714.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IYE22_001499"
     CDS             complement(14935..15867)
                     /locus_tag="IYE22_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506715.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IYE22_001500"
     CDS             15984..17063
                     /locus_tag="IYE22_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ9506716.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IYE22_001501"
     CDS             17060..17689
                     /locus_tag="IYE22_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9506717.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
BASE COUNT         5332 a         3697 c         3192 g         5519 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttagctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg
//
LOCUS       DACIRY010000051        17008 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.052, whole genome shotgun sequence.
ACCESSION   DACIRY010000051 DACIRY010000000
VERSION     DACIRY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.052"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..2435)
                     /gene="parC"
                     /locus_tag="IYE22_001502"
     CDS             complement(<1..2435)
                     /gene="parC"
                     /locus_tag="IYE22_001502"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ9506718.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPE"
     gene            complement(2447..4492)
                     /gene="parE"
                     /locus_tag="IYE22_001503"
     CDS             complement(2447..4492)
                     /gene="parE"
                     /locus_tag="IYE22_001503"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ9506719.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(4658..5092)
                     /locus_tag="IYE22_001504"
     CDS             complement(4658..5092)
                     /locus_tag="IYE22_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ9506720.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5219..5857
                     /gene="plsY"
                     /locus_tag="IYE22_001505"
     CDS             5219..5857
                     /gene="plsY"
                     /locus_tag="IYE22_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ9506721.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(5989..6864)
                     /locus_tag="IYE22_001506"
     CDS             complement(5989..6864)
                     /locus_tag="IYE22_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ9506722.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(6946..7737)
                     /gene="codY"
                     /locus_tag="IYE22_001507"
     CDS             complement(6946..7737)
                     /gene="codY"
                     /locus_tag="IYE22_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ9506723.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(7755..9155)
                     /gene="hslU"
                     /locus_tag="IYE22_001508"
     CDS             complement(7755..9155)
                     /gene="hslU"
                     /locus_tag="IYE22_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ9506724.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(9169..9717)
                     /gene="hslV"
                     /locus_tag="IYE22_001509"
     CDS             complement(9169..9717)
                     /gene="hslV"
                     /locus_tag="IYE22_001509"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ9506725.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(9754..10659)
                     /gene="xerC"
                     /locus_tag="IYE22_001510"
     CDS             complement(9754..10659)
                     /gene="xerC"
                     /locus_tag="IYE22_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ9506726.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(10857..12233)
                     /gene="trmFO"
                     /locus_tag="IYE22_001511"
     CDS             complement(10857..12233)
                     /gene="trmFO"
                     /locus_tag="IYE22_001511"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ9506727.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(12202..14280)
                     /gene="topA"
                     /locus_tag="IYE22_001512"
     CDS             complement(12202..14280)
                     /gene="topA"
                     /locus_tag="IYE22_001512"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ9506728.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(14402..15259)
                     /gene="dprA"
                     /locus_tag="IYE22_001513"
     CDS             complement(14402..15259)
                     /gene="dprA"
                     /locus_tag="IYE22_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ9506729.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(15315..16082)
                     /locus_tag="IYE22_001514"
     CDS             complement(15315..16082)
                     /locus_tag="IYE22_001514"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ9506730.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(16075..16935)
                     /gene="ylqF"
                     /locus_tag="IYE22_001515"
     CDS             complement(16075..16935)
                     /gene="ylqF"
                     /locus_tag="IYE22_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ9506731.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         4329 a         3800 c         2959 g         5920 t
ORIGIN      
        1 atctcaggag catcgataac ttcgatgact tctccacctt ttttctcatc catcgcaaat
       61 gatccattcg atgttcgatc acctataggg aaatcagttg ggtcaatcgt taactgctgc
      121 cctttttgat tcatcaaggt tatttctttt gatcttcctt ccgacatata gataatacgg
      181 tgcggatttt ttttcagctc acgcatgacc gtcactcccc gcttcgcacg tgaagtttgg
      241 ctgatttctt gagcaagcat gcgtttaaca gctcctcgct gagtaacgat tacgatcggc
      301 gtatcacctt ctgctaacac aagcagcccg ttgataatcc tatctccatc tttcaaattc
      361 atagacttca ctccagctgc ctttgctcca acaacgggaa catctgttaa aggatagcgc
      421 aaacccattc cttgatgcgt tactaagaaa acatctaagc ctggctgatc ttctttcaga
      481 tacacagcaa tcagttcatc ttcttccgcc tttagcttca tacagctgag cggacggctc
      541 ttataggtgc gccatggttc gaactctgtc attcgtgttt gcttgatcaa tccattcttg
      601 ctgatgaata caaatgtttt ctctgcttga attttttgat atgggaaaac agctaaaatc
      661 gattcgtcta cagacagatt ggagattgtt tgagaaatgt gctcaccagc atctttccat
      721 ttcaaatcgg gtagttcatg gacagggcgg tagatcacat ttgctttatt tgtgatcaga
      781 agaacatggt ccaatgtact caattcacct gagtatagca agtagtcgcc ttctcgcatc
      841 ccgatttctt ctggtttgga agcactataa gaacgaaggc ttgttcgctt cacatagcct
      901 tctcgcgtca cactgacaat tacgtcttct tgtgccacta agacttgcgt atcaattttg
      961 atttcttcga tttcgtcttc gattgtcgtt agacgagcgg aagaataatt ttttttcact
     1021 tctcgcaatt cttttttcat aacagagaaa agctctttgt catttgaaag gattttattc
     1081 aactctgtga tttgcgcaat caagctttcg gattcctttt gaagctctgt gatatccgtg
     1141 ttcgtcaaac ggtataattg caatgtgaca attgcttctg cttgttcttc tgtaaattga
     1201 aagacgtcga ctaaattctt tttggcgtct tttttgtctt tactttcacg aatcgttgcg
     1261 attacttcat ccagaatcga taaagctttc atcaatcctt ctacgatatg ctgtcttttt
     1321 tgagctttgg ctaaatcgaa ttgactacgt tttgttatga cttgctttcg atgctcgata
     1381 taacttctta aaatgtcttt cagtccaact tgatgcggag tcatatggtc gatagcgacc
     1441 atattgaaat tgtaattgat ctgtagctca gtgtttttga acaaataatt caaaattcct
     1501 tgagcattgg cgtctttttt caattcgacg acaatttgca gacctgttct atcactttca
     1561 tctcgtactt cagctattcc atcgattttc ttattcaaac ggatctcatc catttttttc
     1621 accaaggtcg ctttatttac ttcatatggg atttctgtga tcacgatttg ctgtttatta
     1681 cccttcattg cctctatctt tgttttcgaa cgaagaataa cttttcctcg tcctgtttca
     1741 taagcttttt tgatttcatc ttttccttgc aagatccctc ctgttggaaa gtcaggtcca
     1801 ggaatatatt ccatcaattt ttcaaggctc gcttgtggat gatcgatcat atagattgtg
     1861 ccatcgatca cttcagctaa gttatgagtc gggatttctg ttgcatatcc agcagatata
     1921 cctgtcgatc cattgacgag aaggtttggg tatcgagctg gaaggactgt tggttctttt
     1981 tctgtatcat caaaattcca cacgagatcg actgtttctt tctcgatatc tgctagcatc
     2041 tctccactta acttggataa tctggcttca gtataacgca tcgcagcagg tggatcaccg
     2101 tccatactcc cgttattccc gtgcatttcg atcagtactt cccgcaactt ccagtcttgg
     2161 ctcatgcgga ccatcgcttc ataaatactg atgtcaccat gaggatgata attccccatg
     2221 atatttccga cagattttgc tgatttacgg aatcctttgt catacgtatt gccatctttg
     2281 ttcatggcaa ataatatccg gcgttgcact ggcttcaatc catcacggat atcaggcagt
     2341 gcacgttctt ggataatata tttggaataa cgtccgaagc ggtcacccat cacttcttct
     2401 aaagttaatt cttgtacttc ttgtcgattt tccatcaggt gacctcctat tctacttcaa
     2461 ataactgatt gttttcattt ttatctgctc gttcttcatc taataaatca ttagaaactg
     2521 aaggagagat ttcagtatcc tcttttttat ctaagatact gccatcttct tcgagcgtaa
     2581 attgcacgtg attttcgatc cattttcttc gaggttctac tttgtcaccc atcagtgtgg
     2641 ttactcgacg ttcagcttgc gcagcatcat cgatacggac gcggatcaac gtacgggaag
     2701 tcggatccat cgttgtttcc cacaattgtt ccgcattcat ctctccaagt cctttatatc
     2761 gctgcagcat atatcctttg ccgacttttt taatcattgc tgccaattcg tcatctgtcc
     2821 acgcatattc aatgacttgc ttctttcctt gtcctttgga tactttatat aaaggcggta
     2881 acgcgatata tactttccca gcttcaatca gcggtttcat ataacgataa aagaaagtaa
     2941 gcagtaatac ttgaatatgg gcgccgtctg tatccgcatc ggtcatgatg attactttgt
     3001 cgtaattaca gtcttcgata gaaaactcag gaccgactcc tgcaccaatc gtatagatca
     3061 tcgtattgat ttcttcattt ttcaagatat cttgcatttt agctttttcg gtattgatga
     3121 cttttcctcg taaaggtaga attgcttgga actttctgtc tcttccttgt ttagcagatc
     3181 cgccagcaga atccccttcg actaaataca attcattttt cttagggttt cgggattgtg
     3241 cgggtgtcaa ttttcctgaa agaagagatt cgcctttttt acgcttcttc ccatttcggc
     3301 tttcttctct ggctttacgt gctgcttctc tcgcttctct ggctttgatt gctttacgga
     3361 ccaacatctg gctcatttcg ctattttcct gcaaataaaa gcccatttgt tcactgatga
     3421 cgttatctac tacggtacga gcaactggcg taccaagttt ttcttttgtt tgcccttcaa
     3481 attgcagaag attttcaggt acacggatag aaagtacagc agctaaccct tctctgaaat
     3541 cactaccttc cagattcttg tcccgttctt tcagcaaacc aacttttctc gcatattcat
     3601 tataagattt cgtcatcgcc gctttcatac cagcttcatg ggttccccca tcttttgtcc
     3661 gaacgttatt gacaaaagat aaaacatttt ctgaataacc gtcattgtat tgataagcga
     3721 cctctacttc gataccttct ttttctcctg aaaaataaac aactggtgtc aatgtatctt
     3781 tttcttcatt cagataatca acaaattctt tgattccttc ttcgtaatga aaaatttcct
     3841 tgactggttc ttctccacga agatcagaaa gagagatttt aacgcctttt agaagaaagg
     3901 cagattctct taaccgttcg gctaaaattt catatgaaaa tttcgttgta ctgaatattg
     3961 tatcatctgg taaaaaatgg acagtcgtcc cgtttgcttt ttttgttttt cctatctttt
     4021 tcaaagtacc gtcaggttta ccgccgtttt taaatttctg ctgatactct acgccatctc
     4081 taacgatcgt tacaattagc catttcgata aggcatttac cacactggct cctacaccgt
     4141 gcagtccacc agaagttttg taccctcctt gaccaaactt tccgccagca tgcagcacag
     4201 taaaaatcac ttcaactgtt ggtattccgg aagcatgcat tcctacaggc atcccacgtc
     4261 cgctatctgc aacagttata ctattgtctt catggatggt cacatcaatc tcactgccat
     4321 aacctgataa tgcttcgtca actgcattat cgacgatttc atagactaaa tgatgcaagc
     4381 ctcggctgtc agtggatcca atgtacatcc ctggtctttt tctaactgcc tctaatcctt
     4441 ccaatacttg gatggacgag tcattgtatt cgttgtttac tttttttgcc aaggcaaacg
     4501 ctccttcaat aatactttgc ggcaaaaccg caccacgatt atcatactag aaaaggtcgt
     4561 gaaaaaaaag agggtagacg agaaatcaat tttaattatc tcctaaccgc tttttcgaca
     4621 ttcccctcta tcgatcatct cttctttgtg gtcattttta tttttggttt tctttcaact
     4681 taccaagttc aatcttaata caacgatcca ttacgatatc tgtacgtcct gcttttctta
     4741 gcatctcagc tgcttctttg ttttcaatcc ctaattgtgc ccagaacact ttagcatcag
     4801 ttttcaaaaa atcacctgct atttcaggta aaaattcact ccgtctaaat acatctacga
     4861 tatcgattgg atcatcgatt tctgtcaaat tcgcatagac tttctcgcct agaatttctt
     4921 caccagccaa taatggattt actggtatga ttttatatcc attgtcttgc aatacctgtg
     4981 caaccatgta acttgttcgt tctggtttat tgctcaagcc tacgacggca atattttttg
     5041 cttcttccaa atagttgaaa atcgtttctt gtgttggatt ttttacagac atttctgcca
     5101 ccccttgctt ttctttttat tccatctatt atacttgtat ctttcatttt ttcaattgtt
     5161 cctgttacct attcatgcta aaataaagga tagttttcaa cttgaaagag ggtaaaaaat
     5221 gaaattgatc ttattactta ttttagcata tcttttaggt tctattcctt ccggtgtctg
     5281 gattggcaaa ttgtttttca aaaaagatat ccgacaacac gggagcggta atactggaac
     5341 gaccaataca tttcgtgtat taggcaaaac agccgggatt gttgtcctgc ttatggatat
     5401 tttaaaaggt acattggcta cttgtttgcc gatgatcttc cacctttctg ggattaaccc
     5461 tctttggttt ggggtatgcg ccattcttgg ccatacattt ccgatttttg ctgggttcaa
     5521 aggaggaaaa gctgttgcga caagtgcagg aatgctttta ggatttaatc ctgtattttt
     5581 tgtttattct tgtatcatct ttatcgttag tttattttgt acaagtatgg tgagtttgag
     5641 cagtatgatc agtgcagtat tgatcacttt atctacgatc gtccttcctt atgtagctcc
     5701 tgtgattttg gctaagccca attggttact gacgatcatt gcctttttag tttcttcctt
     5761 tatcttttat cggcataaag acaatatcaa gcgtatcaga aaccatactg aaagtcgaat
     5821 cccgtttggt ttgaatagca gcaagaaaaa atcctgatac tttttcattc caaagagata
     5881 aaacgggagt gtgacaaatc gatgtcacac tcccttatta cttacaggat agctttttta
     5941 cacaccttgg taaacggtgt tccctgctaa cttctcggtc acaacctctt acttcactgt
     6001 gatggaatat tttgtcttga agatttcatg ggcacctagt tgattgatcc ctactttttc
     6061 catcaagttt ccactactgt tcaccgtgtc agcgattccg caccacggtt cgatacaaac
     6121 gaaaggcgct tcttttggat aaggagacca gatccctaca taaggcatat ctttatagct
     6181 taaagttacg ctatgggaag atttttcact tcgaatggcg tacgcattta gaccccttgt
     6241 ttcaaaaacc aaggcatctt gctcaaaaag ttcacgattc aaagccagat tcgtatttgt
     6301 ctgacctaac gtcttttgat ccatatcgat gaatgggcct ttcaatggta ggcgtaatct
     6361 tgatttcaat ggagaaaatg ccagatagaa atcttcaaat gacaatcctt cttcaagagg
     6421 tacattgaaa gcaggatgtc caccgataga aaagaacatt tctttttcac cggtatttgc
     6481 cacttgataa tgaaccgtga tgccgtctcc tcctaattca tagctgatca ctaattcaaa
     6541 atcaaatgga tacttttcta atgtctccgg cgtactcctt aagatgaaac tagccatttc
     6601 ttgtccatgt tcaatgactt caaaatccat atcccgagca aatccgtgtt gccccatttg
     6661 atatgttttc ccttgataaa catactgatc atcttttaaa cgtccaacaa aagggaataa
     6721 aaccggtgca tgtctgcccc aatacgctgg atcaccttgc cagatatact ctaaaccatg
     6781 ttcttttgat ttcagactga tcaattccgc accgtgttca gcgatttcgg cgatcagctg
     6841 gtcattttga ataataacag tcattgccat tcctccaact tcgacttact ttttaaaatg
     6901 ggaaggctgg aacaaacatt tctatctgcc ccagtcccct tacttctaat aactgtgttt
     6961 tgctaactcg tctttaaaac gggaattcaa tacttttaaa taagttccct tcatgcctaa
     7021 cgaacgagat tcaatgattc cagctgattc caattttcgt aaagcattga cgatcacaga
     7081 acgagtgatg ccgatactat cggcaatatt agaagcagtc agacgacctt catctccatc
     7141 caatgcttca aagatagctt gtacagcctt caattcgcta taagataatg tgttgattgc
     7201 catttggaca gccgttgcac tccgtacatc tgcttcgatg cttcgagact tctgataaag
     7261 gatctgcata ccgacaaccg tagctccata ttctgctaag actagatcgt catctgcaaa
     7321 cgattgttcc atacgagata agataatcgt acccaaacgt tcgccagctc caaagatcgg
     7381 aacgacagtc gtcagaccaa atggatattt ctctcttgac tctaccggaa atgcagtaat
     7441 atcactagta attgggatat tagcttcagt tttcatcaac tgtgccactg tatcagtata
     7501 agattcagga aattgtttct caacaaacat tgttttgatt cgttcattgt tcacatcgtg
     7561 acgttcatta taacctagta atactccgtc acttcctatg atataggtgt tgctgtcaag
     7621 aataccacct agtgtgacag ccattttatc gtaaggcaaa tctgcttgca tgtcaaaagt
     7681 attcttttgc tgcaaaagtg tgtttatctt gcgggttttc gttagcaagt ctgtcattgt
     7741 cgtcctccga ttttttataa aatatagcga cttaaatctt catttgccgc aattgagcca
     7801 agtttgtcat ccacatatgc ttctgtgatc gttatttctc ccatttgcat atctggtgct
     7861 tcaaataata gatcttctag tagtttttcc aaaatggtat gcagtctgcg tgcgccgata
     7921 ttatctgtgt cacggttgac atcgaaagcg atttgagcga tccgttcaat tgcttctttt
     7981 gtaaacgtaa ctgacacatt ttctgtaccg atcaacgcga cgtattgttt tactaaagca
     8041 ttatttggtt cggtcagaat cttcacaaaa tcttcagctg tcaaatcatc taattctacc
     8101 cgtatcggga agcgtccttg aagttctgga attagatcac ttggtttaga caaatggaaa
     8161 gctcctgaag caatgaatag gacgtgatct gtttggatga tcccgtattt tgtgtttact
     8221 tgtgaacctt cgacaattgg caatatgtct ctttggacgc cttctctgga tacttctcca
     8281 gattgctgtg atttagaagt gatcttatcg atttcatcga taaaaataat tccactttct
     8341 tccgctaatt tgattgcctc actatggata tcagcatcct tgactaattt tgctgattct
     8401 tccttgatca ataactctaa agcttctttg actgttaccg tccgttccac ttttttcttc
     8461 ggtgtcaaag cacctagagt ttcatttagg tcgatcccca tttgctccaa tccattgttc
     8521 atcattggtg caggtttctt ttggtcttca acttcaatgg ttacttcacg actatccagt
     8581 aaaccctttt gtagttgttc caagactgta cggcgattga ttcgaatatc ttctgtcact
     8641 tcttcttgcg gttcagcggt attttgctga ctagcattaa aaagattcat catttgttca
     8701 aattgattag tggattgttt ctgttctttt ttgataccag gtaccaagat ttttaccaaa
     8761 cgtttttctg catttttttt agcacttgtg tatacacggc tgtactgctc tttttctacg
     8821 atttggatcg cattttctac taaatcccga accattgatt ctacatctct tccgacataa
     8881 ccgacttcag taaattttgt tgcttcgact ttgacaaatg gtgcattgac gatttttgcc
     8941 aaacgtcgtg cgatttctgt tttaccgacc cctgtaggtc cgatcatcag catatttttt
     9001 ggtgtaatat cttgttgcat tttttcatct aactgcaaac ggcgatagcg gttacgcaaa
     9061 gcaacggcaa cagatttttt tgctgcgtgc tgtcctacga tatattcatc taattcactt
     9121 acgatttgtc ttggtgtttg ttttaattca tacatatcta ctcacctctt acagttcttc
     9181 tacgataata ttatgattag taaagataca gatatctgcg gcaatattta acgcattttc
     9241 tgcaatttct ttcgcactca tgtcgttttg agcatagttt ttcattgctc gggctgcagc
     9301 caacgcaaaa ttccctcctg atccgatagc taagatacca tcatctggtg tgataacttc
     9361 tcctgtacct gaaacaagaa gcatttcttt atcattcatt acgatcagca tcgcttcaag
     9421 tttttgcata gattgctgtg tccgccattc ttgagctaat tcgacagctg cgcgttgtag
     9481 attcccgtta tattcgttta gtttgccttc aaatttttct tctaaagtaa acgcatctgc
     9541 aacacttccg gcgaaaccta caacaacttc tccattgtag atacgtcgta ctttctttgc
     9601 agtacctttc atgacaacag attcacccat tgttacttgg ccatctccag ccatagcaaa
     9661 tttcccgtct ttttcgacgg cacagatagt ggttgaatga aatggtgatt cgaccatgtt
     9721 tgtttcctcc taaatttgtt cttttcctta tttttacgct cttggatgga aagaacgata
     9781 gtttttttgt aaactttctt ttgttacatg ggcatagatc tgggtagtcg ataaattggc
     9841 atgtcctagc aattcttgga ctgtccgcaa atctgcccca ttatttaata aatgagtagc
     9901 aaatgtatgg cgcagcatgt gaggatggat atcactatcc aaactgcttt ttttgatcat
     9961 ttgattcaag acatattcaa ttccagtagg cgtgattggt tttccatgat gattcacaaa
    10021 aacgatatca tgctcttcgt gatattttgt catcaatacc tggcggcctt ccctgaaata
    10081 ttcttgcaga gcatcttgag caaaagaacc tagaggagca tatctctctt tgttcccttt
    10141 accatggatc aacatgacct ctgaatcaaa atcaatgtct gataccgaca aactgctaca
    10201 ctcacttaaa cggatgcctg acccgtaaag gacttccagt aaagcgcggt tcctcaaatc
    10261 caaaggacta tctcctgcta cgctgtcaaa aagtgcttgc atttcatttt cataaaaaaa
    10321 acgtggcagt ctcaaatttt tctttttgag atgaacgtaa gaaaatggat tttcttttat
    10381 cacttcttct ttgagaagat actgataaaa cgaacgtagg ctggctatct tgcgactgat
    10441 agaatttctg ctatagctag gacgatgatc agctaatgca ctcaaataga cacgaaccgt
    10501 aaaatattcc gccttcagca gatcagcgtc tccagattcc tccaaaaaat gtttgaaatg
    10561 gagaatgtct tcttcatatg cttctttcgt tttttctgaa tatcctcttt cgacaatcaa
    10621 ataacgcaaa aatttttgtt cccacgtggc ttctgtcatt ttctcacctt ccttttgtcc
    10681 cagagaataa tttagcacag cgtttataaa aagacaaata aattgtcaga atttaaccaa
    10741 tatttctaag tttgttcaca attggcgcaa ttcctcacat tttgtttgag aaattcttaa
    10801 gaaaaacgca agaaaaagag cgagactatc cgtctcaccc tcttcatgga gtttccttac
    10861 tcatttggga ttgcagtttt ttctaattct gtttttattg cctcattagc ttttaaagct
    10921 cgttgtgcaa ttgcttcata tcttgctttt ttgtctcgaa tgcgctctgg cagttcagga
    10981 aacagaccat agttggcatt catcggttgg aaatgttttc cttccgcatg agtaatgtag
    11041 taagccatac ttccaatagc tgtttcttgt ggaaactcga ctggttccat ttcttttgct
    11101 aagcgtgcgg cgttgattcc agcaagtagt ccgctcgctg cactttcaac atagccttct
    11161 acaccagtca tctgacctgc aaagaacaga tcttcgcgtt tcgttgattg gtaagtcggt
    11221 ttcagcaatt ctggcgaatt cataaagctg tttcggtgca tcacaccata gcgaacgaat
    11281 tcagcatttt ctaacccagg aatcatccgg aaaacacgtt tttgctcgcc ccatttcaag
    11341 tgagtctgaa agcccacgat gttgtataaa gaagctgcag cattgtcttg acgcagctgg
    11401 ataacagcat aaggacgttt gccagtttta ggatcttcca atccaactgg tttcataggt
    11461 ccgaaaagca tcgttttgaa tcctcgctgt gccattactt caattggcat acatccttca
    11521 aaatattttt ctttctcaaa agatttaagt tctgccactt cggcattgat tagtgcctca
    11581 tggaaagctt tgaattcctc ttctgtcatc ggacagttca agtaagctgc ttctcctttg
    11641 tcatagcggg acttcaaata tactttatcc atatcgatcg tagctttgtc tacgattggt
    11701 gcagctgcat catagaaata aaaaccatct gaaccattga atgctttgat gctttctgct
    11761 aatggatgag aagtcaaagg tcctgttgct actacggtga ttccttccgg tatttcagta
    11821 atttcttcgt ttatcactgt tactaatgga tgctctttga cttttcgggt aattgtttca
    11881 gaaaaagaat cgcggtcaac agctaaggca ccaccagctg gcactgctgt ttcatctgcc
    11941 gaagtgatta ctaccgaatt caatcggcgc atttcttctt tcaataaccc tactgcatta
    12001 gccaaactgt ttcctcttaa tgaattggaa catactagtt cagcaaaatt acttgtgtga
    12061 tgcgcctcag tcgatttgac tggacgcatt tcatataaac ggacagggac tcctgcttgt
    12121 gcgatctgcc aagcagcttc acttccggca agccctgctc caacaacatt taccattttc
    12181 aatgaaagaa cctcttttct attatttttg gatgtcttct tcgtaatctc catttacaca
    12241 gactacttgt ttaccgcctt tgactttctt ctcgaccaga tatccgtcac attttggaca
    12301 atttctgccc actggtttat cccaagaagt gaattcacat tctgggaaac gggaacaacc
    12361 atagaatagt ctgtttttct tagattttcg ttcaatgact tgtccttcat gacatactgg
    12421 gcaagtcaca ccgatttctt ttacgattgg tttcgtattc cgacaatcag gaaaattact
    12481 gcaagcataa aacttcccat atttccctaa ttttatcacc atcggatgtc cacagacatc
    12541 acaatcgaat cctgctggtt catctttgat ttgaattttt tcgatttttt cttccgcatt
    12601 cgtcagttct ttttcaaacg gtttatagaa acgatcaacc actttgaccc acgcttccgt
    12661 gccttcttca attttatcca aatctccttc catttctgct gtgaaatgga tatcgacgat
    12721 ttgagggaag aattcacaga ttaatgaatt gacaatttct cctagttcag tcggctcaaa
    12781 tcttttttgt gtcaatttta catagtagcg tctttggatc gtatcaagtg ttggtgcata
    12841 agtggaaggt cgccctaccc cgttttcttc tagtgcacga atcaacgtag cctcactaaa
    12901 acgtgctggt ggttgagtaa aatgttgttt tggttcaata tcaacagatt ggactacatc
    12961 gccttcttca agttctggaa gaatattttc tttctcttct ttgccgtcat ctctaccttc
    13021 cacatacact tgcatgaatc ctttgaattt gatttttgaa ccgttcgcaa tgaagatgac
    13081 accattttgt tctaatgtca ctttcattgt atctaaaata gctggtgtca tctgactagc
    13141 cacaaaacgt gaccagatca atgtataaag tttcaattga tctttatcta aatattgttt
    13201 gagttcatct ggtgtacgaa gcacactcgt tggacgcaca gcttcatggg catcttgtgc
    13261 gccttgagca tttttgatct ttcggtgacc atgtctagaa aattcttttc catacgtatc
    13321 ttcaataaat gctgcggcct ctgcttttgc tgtatcggca atccgggtag aatcagtacg
    13381 catgtatgta atcaagccga ctgttccttg tttacccaaa gtgattcctt catataattg
    13441 ctgtgctacc atcatggttt ttcttgttcg aaagtttaac ttacgggcag cttcttgttg
    13501 caggctactt gtagtgaaag gattcgcagg gtttcttttg cgctctttct tttctacttt
    13561 tttaacttcg aagtctgttc catcaattct agcagtgact tcttttacac tttgtgcatc
    13621 tggtaatttc tttttcttac cgtcgattcc ccagaaattt gctttgaatt tcttgcgagc
    13681 ttttttaaaa ttaccgtcga tgctccaata ttcttctgga acaaattctc ggatctcttt
    13741 ttcacggtca ataataattt ttaaagcgac agattggaca cgtcctgcac ttaacccttt
    13801 cttgacctta cgccagagaa taggactgat cgaataacca acgattctgt ctaaggtacg
    13861 acgagcttgc tgtgcatcta ctaaatctac atcgatcgtt cttggttcct taaaagctgc
    13921 tttgactgcg tcttttgtga tttcattgaa gaccacccga tttttttctt tcagatccaa
    13981 cccaagtagg tacgctaaat gccaagcaat cgcttctcct tctctatccg ggtcacttgc
    14041 caagtaaact ttttgagctt tttttgctgc ggctttcagg cttttgatga catcgccttt
    14101 tccgcgtata gaaatataat ggggttcata attgttttca aaatcgatac ccattttact
    14161 tttcggtaaa tctcttatat ggccaacact ggccattact ttatagtttc gtccaagata
    14221 tttctcaatt gtcttggctt ttgcaggtga ttctacaatc actaaatatt tatacgccat
    14281 tactgaatgg actcctttcc attgtcgtgt tcctactatt aaaagaggat tcatacaaat
    14341 cgtagctaat catatccatt cagaaaacgt ttgtcaagaa aagcaaatga aaagtatggt
    14401 gttattcttt aaattctgcc aagatatctt cacccgaaac cgtgcaaact gcaccgtctt
    14461 ggatcaaatg atgacatccg gtagaatgtt ctgttaaaat atttcctgga acagaaaaaa
    14521 ctgttcttcc aagatccaat gcttgctgtg ctgttatcag agagccgctt tttttcttgg
    14581 cttcaaagac acatgttcct ttagacaaag ctgcgataat ccgatttctc attggaaaac
    14641 aatgcttttt cgggccggat tgaggaggat attcactcaa aagaagatga tcatttgcaa
    14701 tcacctcttg caagtcacga ttttcagcag gatagacttg ctctatccct gttccaatga
    14761 ctgcgattgt ccgtccacca tgagagattg ctgcttggtg accgtatgta tctatccctt
    14821 tcgcgcagcc actgacgatc acatatccag cttctatcac tggttttagt aaataataaa
    14881 ctgttttttt ggctaaagca gaagtgtgtc ttccaccaac aacgcttaac atgttacaag
    14941 aaagtaaaga tagatcacca ttataaaaca gtacaattgg cggataggtc aagtgaagca
    15001 gctgaggggg atatttggga tcaaagcaag tgataaacga ctgtttcgat tggattttgc
    15061 cccagttagc gtgaatttcc tgccaacttt tgcgaaacag ctcccggtgt ttggttattt
    15121 tacctatctt aatgatgtct tcaaatccga tttctttata aggatactgc atcatccagt
    15181 tagcaagtgc ccattttcct tgactactga ttccttttgt ataagcaagt ttcaataaat
    15241 tttcttctat ttgatacatc ataaaaccgc ctttcgtctc tctttctaat aagatacgta
    15301 aaagcggctg ttttttattg taaatatttt tttacaggtg caaaactttt acgatggatt
    15361 ggcgtaattc cttgtcgttc aagtccttct aagtgttcct ttgttccata acctgcatta
    15421 tgcgagaaac catatccagg ataaagcacg tcatattctt tcatcaattg gtctcgatat
    15481 actttagcaa tgatacttgc agcagcaatc gacacagatc gtgcatcccc tttgattatt
    15541 ttttcctgct gaataggcaa atccaaggtc atcgcatcga tcaacaaata gcttggtaca
    15601 atggacaatt tttctactgc ttggatcatc gctaatttac ttgcttcata aatattcaca
    15661 tcatcgattg tctgctgatc aacgatcccg atcccgattg ccagggcttt ttcttgaact
    15721 tgttgataaa gaagttctcg ctttttctct gataattgtt tggagtcatt cagccctaat
    15781 atgatctcat ttttcggcaa gataactgca gctgccacga caggtccagc taatggtcct
    15841 cttcctactt catcaatgcc ggcaatccat tggtgacctt gtgcgtaccc ttgcttttca
    15901 aagcaaaaca tttcttcata tttttcctta attaatgctt cctttttcaa tcgtcgttca
    15961 aaagaagcca gtgcttgctg cacgcctttg cgtggatcat ttcttaatga ttctagtgta
    16021 ggatcatcta ttctctgtat gcttttcaat cgttctttga tactttggat ggattcagtc
    16081 atcacttttt tgctccattt cttcgcaacg atccagtgtg tatcgtccaa gttttccttg
    16141 acgaatatca tgcacgacta attcgctgcc acgatcatag tcttctttat acccacgttt
    16201 tgctgtaata tccatcagta attccggtgc caatttttct tcttgctcga ttgataaacc
    16261 ataacgatcg ctgattcttc ctgaataata tcgggcaaaa aagtctaatc cgtaaatcgc
    16321 taaatcatct aaatggacaa gctggtcttt gatcgcgcct gtcagcgcta gttttttgcc
    16381 gatttcttca tcttcaaatt tgggccataa aatccctggt gtgtctaaca attccaactc
    16441 attacctaaa cgaagccact gctgaccttt tgttacgcca ggcttatttc ctgtctgtgc
    16501 aattttcttg cctactagtc gattcatcaa cgtagatttc ccaacatttg gaatacctaa
    16561 gaccattgcc cgaatagcgc gaggcttgat cccccgagca gcatctcttg cccgtttttc
    16621 tttcaatgct tctttagccg caggtactag acctttaatt cctttacttt cttgtgccgt
    16681 gatagctaat gcaagatggc cttgtttacg aaaatattct tgccacagct ttgtctgttg
    16741 tggatctgcc agatccgctt tattcaataa aacaaggcga ggtttttgct gaatgatctg
    16801 atctagcatt ggattacgag aagataaagg taatcgtgcg tctaccaatt caaatacgat
    16861 gtccacatat ttcatttttt cagaaacttc tcgtcgggct tttgccatat gtcccggaaa
    16921 ccattggatg gtcattgttc tctccattcc tttcataccg gaatattccc cggctttact
    16981 tgtttttttc tttttcaata taaattca
//
LOCUS       DACIRY010000052        16506 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.053, whole genome shotgun sequence.
ACCESSION   DACIRY010000052 DACIRY010000000
VERSION     DACIRY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.053"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1338)
                     /locus_tag="IYE22_001516"
     CDS             complement(<1..1338)
                     /locus_tag="IYE22_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9506732.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFY"
     gene            complement(1335..2021)
                     /locus_tag="IYE22_001517"
     CDS             complement(1335..2021)
                     /locus_tag="IYE22_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9506733.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2214..3635)
                     /gene="gndA"
                     /locus_tag="IYE22_001518"
     CDS             complement(2214..3635)
                     /gene="gndA"
                     /locus_tag="IYE22_001518"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ9506734.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(3805..3984)
                     /gene="rpmF"
                     /locus_tag="IYE22_001519"
     CDS             complement(3805..3984)
                     /gene="rpmF"
                     /locus_tag="IYE22_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ9506735.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4042..4611)
                     /locus_tag="IYE22_001520"
     CDS             complement(4042..4611)
                     /locus_tag="IYE22_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ9506736.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            4797..5699
                     /locus_tag="IYE22_001521"
     CDS             4797..5699
                     /locus_tag="IYE22_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9506737.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5734..7518)
                     /gene="pyk"
                     /locus_tag="IYE22_001522"
     CDS             complement(5734..7518)
                     /gene="pyk"
                     /locus_tag="IYE22_001522"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ9506738.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7592..8554)
                     /gene="pfkA"
                     /locus_tag="IYE22_001523"
     CDS             complement(7592..8554)
                     /gene="pfkA"
                     /locus_tag="IYE22_001523"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ9506739.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8722..12036)
                     /gene="dnaE"
                     /locus_tag="IYE22_001524"
     CDS             complement(8722..12036)
                     /gene="dnaE"
                     /locus_tag="IYE22_001524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ9506740.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12267..12452
                     /locus_tag="IYE22_001525"
     CDS             12267..12452
                     /locus_tag="IYE22_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ9506741.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12514..13710)
                     /locus_tag="IYE22_001526"
     CDS             complement(12514..13710)
                     /locus_tag="IYE22_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ9506742.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(13880..15316)
                     /locus_tag="IYE22_001527"
     CDS             complement(13880..15316)
                     /locus_tag="IYE22_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ9506743.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            15609..16418
                     /locus_tag="IYE22_001528"
     CDS             15609..16418
                     /locus_tag="IYE22_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9506744.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4843 a         3464 c         2913 g         5286 t
ORIGIN      
        1 ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg
       61 aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt
      121 agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg
      181 ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg
      241 atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc
      301 attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa
      361 gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg
      421 atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt
      481 ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat
      541 acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat
      601 ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag
      661 taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat
      721 caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa
      781 agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata
      841 tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt
      901 acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc
      961 caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat
     1021 cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt
     1081 cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa
     1141 acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag
     1201 attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa
     1261 agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga
     1321 ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag
     1381 ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg
     1441 tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat
     1501 accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca
     1561 attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa
     1621 gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat
     1681 gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct
     1741 ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt
     1801 ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc
     1861 atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg
     1921 ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt
     1981 gccaagttct tttcatcttc aatgattaga atattgctca tttattattt tatcctttct
     2041 attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag
     2101 tcattttatt atggatagca attttaaaaa agagctgaag tctactcata acaatcgact
     2161 tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct
     2221 tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg
     2281 ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa
     2341 tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag
     2401 gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct
     2461 agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat
     2521 cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc
     2581 caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt
     2641 ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac
     2701 tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca
     2761 gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc
     2821 gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct
     2881 actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct
     2941 atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct
     3001 actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct
     3061 ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata
     3121 tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca
     3181 actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct
     3241 tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca
     3301 ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta
     3361 tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt
     3421 agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct
     3481 ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg
     3541 aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg
     3601 gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt
     3661 tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
     3721 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
     3781 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
     3841 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
     3901 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
     3961 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
     4021 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
     4081 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
     4141 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
     4201 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
     4261 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
     4321 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
     4381 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
     4441 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
     4501 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
     4561 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
     4621 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
     4681 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     4741 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     4801 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     4861 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     4921 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt
     4981 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     5041 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     5101 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     5161 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     5221 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     5281 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     5341 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     5401 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     5461 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     5521 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     5581 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     5641 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     5701 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     5761 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     5821 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     5881 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     5941 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     6001 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     6061 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     6121 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     6181 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     6241 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     6301 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     6361 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     6421 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     6481 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     6541 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     6601 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     6661 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     6721 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     6781 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     6841 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     6901 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     6961 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     7021 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     7081 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     7141 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     7201 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     7261 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     7321 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     7381 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     7441 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     7501 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     7561 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     7621 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     7681 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     7741 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     7801 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     7861 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     7921 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     7981 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     8041 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     8101 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     8161 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     8221 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     8281 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     8341 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     8401 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     8461 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     8521 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     8581 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     8641 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     8701 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     8761 aagttgccgc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     8821 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     8881 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     8941 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     9001 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     9061 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     9121 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     9181 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     9241 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     9301 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     9361 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     9421 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     9481 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     9541 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     9601 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     9661 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     9721 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     9781 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     9841 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     9901 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     9961 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
    10021 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
    10081 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
    10141 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
    10201 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
    10261 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
    10321 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
    10381 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
    10441 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
    10501 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
    10561 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
    10621 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
    10681 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
    10741 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
    10801 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
    10861 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
    10921 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
    10981 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
    11041 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
    11101 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
    11161 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
    11221 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
    11281 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
    11341 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
    11401 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
    11461 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
    11521 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
    11581 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
    11641 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
    11701 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
    11761 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
    11821 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
    11881 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
    11941 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
    12001 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
    12061 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
    12121 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
    12181 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
    12241 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
    12301 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
    12361 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
    12421 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
    12481 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
    12541 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
    12601 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
    12661 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
    12721 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
    12781 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
    12841 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
    12901 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
    12961 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
    13021 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
    13081 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
    13141 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
    13201 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
    13261 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
    13321 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
    13381 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
    13441 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
    13501 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
    13561 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
    13621 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
    13681 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    13741 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    13801 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    13861 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    13921 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    13981 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    14041 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    14101 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    14161 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    14221 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    14281 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    14341 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    14401 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    14461 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    14521 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    14581 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    14641 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    14701 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    14761 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    14821 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    14881 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    14941 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    15001 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    15061 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    15121 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    15181 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    15241 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    15301 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    15361 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    15421 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    15481 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    15541 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    15601 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    15661 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    15721 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    15781 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    15841 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    15901 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    15961 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    16021 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    16081 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    16141 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    16201 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    16261 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    16321 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    16381 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    16441 aagaggttgt gacaattttt gggctctata atttatggga ctgatgaatc agaattgatt
    16501 cttcag
//
LOCUS       DACIRY010000053        16203 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.054, whole genome shotgun sequence.
ACCESSION   DACIRY010000053 DACIRY010000000
VERSION     DACIRY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.054"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(61..558)
                     /locus_tag="IYE22_001529"
     CDS             complement(61..558)
                     /locus_tag="IYE22_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ9506745.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(688..1398)
                     /gene="budA"
                     /locus_tag="IYE22_001530"
     CDS             complement(688..1398)
                     /gene="budA"
                     /locus_tag="IYE22_001530"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ9506746.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1411..3075)
                     /gene="alsS"
                     /locus_tag="IYE22_001531"
     CDS             complement(1411..3075)
                     /gene="alsS"
                     /locus_tag="IYE22_001531"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ9506747.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3280..3942)
                     /locus_tag="IYE22_001532"
     CDS             complement(3280..3942)
                     /locus_tag="IYE22_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ9506748.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3952..4767)
                     /locus_tag="IYE22_001533"
     CDS             complement(3952..4767)
                     /locus_tag="IYE22_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9506749.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5029..5472)
                     /locus_tag="IYE22_001534"
     CDS             complement(5029..5472)
                     /locus_tag="IYE22_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ9506750.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5606..5944)
                     /locus_tag="IYE22_001535"
     CDS             complement(5606..5944)
                     /locus_tag="IYE22_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9506751.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5932..6309)
                     /locus_tag="IYE22_001536"
     CDS             complement(5932..6309)
                     /locus_tag="IYE22_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9506752.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6533..7726
                     /locus_tag="IYE22_001537"
     CDS             6533..7726
                     /locus_tag="IYE22_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9506753.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7892..8314)
                     /locus_tag="IYE22_001538"
     CDS             complement(7892..8314)
                     /locus_tag="IYE22_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9506754.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8904..9359
                     /locus_tag="IYE22_001539"
     CDS             8904..9359
                     /locus_tag="IYE22_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506755.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(9521..10693)
                     /locus_tag="IYE22_001540"
     CDS             complement(9521..10693)
                     /locus_tag="IYE22_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9506756.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10899..12328)
                     /locus_tag="IYE22_001541"
                     /pseudo
     CDS             complement(10899..12328)
                     /locus_tag="IYE22_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(12325..13827)
                     /locus_tag="IYE22_001542"
                     /pseudo
     CDS             complement(12325..13827)
                     /locus_tag="IYE22_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(13824..16151)
                     /locus_tag="IYE22_001543"
     CDS             complement(13824..16151)
                     /locus_tag="IYE22_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9506757.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4618 a         3242 c         2866 g         5477 t
ORIGIN      
        1 actgtctttc cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt
       61 ttagttatta ataaatgaaa aagcatttat attaaaattt gctaataaaa gatctacata
      121 tttttctaga aatgcttgat cgatttcatc ataccctttc gttactccgc aatcgatatc
      181 taaaacaccg attaaattac cttcttttac cattggcact acgatttctg attgtgcggc
      241 tgaatcgcag gaaatgtaat ttgcatgggt tttgacatca tcaacgatca atgtttcttg
      301 tttttcagct gattctccac aaactccttt tcccatcttg atacgtgtgc aagaaactcg
      361 cccttggaac ggtcctaaaa tcaattcatt accatcgaat aaataatatc ctgcaaagac
      421 agtctcaggc aaagtctcat tcaacaatgc agatgaattt gctaaatttg ctaaccagtt
      481 atcttctgct tctaataatc cttgttgctg taataataaa atttcgtatg catctttttt
      541 ctgtttttct gtccacatga taaaaaccgc tcctttttga tctatggaga agattatagc
      601 tcagtttttt tgtaaacgct aggttttctc ctcaaaaaaa cgagatacgt caggttaatc
      661 accttgaacg catctcgctg tctttttcta ttctgcttgt tggatttctg ctgctaaatc
      721 agcataatca atcgtttcgt gaacaaaagt cggatcatca actggaaaat gctgtacaag
      781 ggacgagatg cggctaatct caacttttcc ttcagccact tcaaaatcca ttacatgtcc
      841 gccaaatgta tgtgtatcat cgataaaatg caggtgaaat cccgctgcag caactccttg
      901 gaataattca ggcgtaaaga acccgactaa tgttccttgg acattgcttt gttggaattc
      961 tggttgagac tctgaaattt ttgctagtct tctgtaaggt ttttcttgct tcggcatgat
     1021 acggatgtgc atatttttga ataatccagt tatcttcact gccaaaaata ggttttcccc
     1081 ggcttctctt actaatatgt cttttttcaa ttgttctcca tctgcagttt ttgagacaga
     1141 aaaactagta tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt cacttccatc
     1201 taattgggca actgagccat ctgatcttcc ttgataagct acgccatcta aaaagatgac
     1261 ttcaccatca agcccatgca atgttcccaa tcccagagtt cctttttcta atatatgagc
     1321 aatcgtttct gttccatcca tcaagccagc catcaaagct cctaatgtcc cgtgctggta
     1381 taagattttt tcagtcatgt cgacacttcc ttaatagaat tgatctggta agattgtttc
     1441 tcccaatttc tcattatcac ggtaatcaat cggaatatca ataattacgg gtccttctgt
     1501 cgcaaatcct tcttctaaag ctttttctaa ttcctctaca cttgtagctc ggattccttt
     1561 agcgccaaaa gcttctgcat atttcacaaa atcaactgga ccaaagtcta cacctgacgc
     1621 ccgctgatat ttcatttttt cttggaactc taccatattg tagtgtccat cattccaaat
     1681 caaatgaacg atattcaatt ttttacgtac cgccgtttct aaatcctggg cagagaataa
     1741 aaatccccca tcgccggaaa ctgatacgat ttgtgtattt ggacgcacca atgcagcaga
     1801 aatcgcccat ggcaatgcaa caccaagcgt ttgcatacca ttactgaata acaaatgacg
     1861 aggttcataa cttctaaagt ggcgtgccat ccaaatataa tgactaccta catctacggt
     1921 aacagtcata tcatctgtta cttttgactg aagcgtgtta ataacttcta aaggatgcaa
     1981 gatacctgct tctccttttg attgaacaat gtcccgctcc gttaattctg cttgaagaga
     2041 tgctaaatat tcttttgcat cttctgacac ttgttgaggc tctaatgaac ctgtcaataa
     2101 atcaagagtg gctgagatat caccgatcaa ttctctttca ggctgcataa aaggatcgat
     2161 ttcagctggc gcttcatcga tcacaatgat ccgcgcatct ttttccgcat tccagtttct
     2221 tgcttcatat tcgattggat catagccaat agcaataact agatcacttc gtttcaaaag
     2281 catatctcct ggttggtttc taaataaacc cacacgacca aagaaatgcg cttctaattc
     2341 acgtgagatc actccagctg cttggaatgt ctctacaaca ggcaatgctg tctttccaac
     2401 taattgcctg atggctaacg tttctttttc acttgatcct cgcataccta ctaatagtac
     2461 cggtagtttt gcttctctaa tgcgttcagc taggtaacgg atatcatctg cagaagcaga
     2521 acctaattta ggatcgctca atggcttgat gacattccct tggacaggtg catccactac
     2581 atcttgcggg atactgataa agctcgcgcc tttttttgaa ctcttagcca tacgataagc
     2641 attggcaatc acttctgaaa ttgtttctgg atcttggatt tctgcactgt attttgtgat
     2701 tggctggaac agtgctgcat tatccatgct ttgatgggtc aacttcaata gatcgcttcg
     2761 ttttacttgt ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc
     2821 tgtagctaag ttactagcac caggaccact tgttgcgata actactcctg gctctcctgt
     2881 aatacgtccg attgcttgtg ccataaatgc agcattttgt tcgtgacgag tcacgattaa
     2941 ttcaggacct tgatcctcta attcattaaa tacaccatcg atttttgcac ctggtatacc
     3001 gaatacataa tcaacatgat gattaatcaa actttctact acagatttcg aaccttgttt
     3061 cattttttcg tccattgaac atcgtccttt ttatgcttta tttttaatta ttagttagaa
     3121 ataatcacaa ataattgata cagtttctaa acctgtttca ttcaaaagta tagcacgttt
     3181 tatttcattt gtttagccat atgtttcaaa aaatgaaaaa gaatgacagt ttttacataa
     3241 aaactgtcat tcttaattga aagtttcaat ttataaagtc tacagatcca tagttctcat
     3301 caatttacga ttgttgaagt aaatcaaacc aaatacgata gaaggaccta gtagaataca
     3361 ccattggggc tttacgaaaa agaagagaaa caaacttgtc aatagtaaaa aatctagtaa
     3421 actcacatac cattttttca acgtatagta gaacgctatc cgtagaactt gatttggttt
     3481 cgacgctgga tttctcacct gaaaatacca gtagttgatg ctaaaggaaa ctgataaaca
     3541 agcaagtaaa acaaacaacg gaatcaacca ttttccataa gaaaaacgta taaaaaagaa
     3601 acaatcaaaa atcattatca cactaactat ccaagcaaat agccaataaa aaaatccctt
     3661 tagccaaaat tggcgaaacg tctttatgaa caaacgtact ggtgaaaaac ttttttcttt
     3721 tgctaattct tctgtcatac ctatcatcac gatgcaacct ggaccaataa aaagtaaaga
     3781 aaaaataaag gaaaagacat tccttccatc tatcgacaaa aggaacgcag ctgctatata
     3841 caagatattg acacaccaaa aacaaaggct acttgccaat gattgataca accaataaaa
     3901 gaattccata taaacatttt tgtcaaaacg ctgctctttc ataatcacct cctattcctt
     3961 cattcctgat gaagcgatcc cttctacaaa atatttttgc atcgctaaaa atataaaagc
     4021 aacaggaaga acagaaacaa ctgaggcagc catgattaat gcgtactctg catcaaatag
     4081 cccaataaac attttcaacc ctaactggat tgttttattt tccgtagaag acaaataaat
     4141 gaatggcccc atataatcgt tccatgtatt aacgaatgtg atgatcgtca aactggcaat
     4201 cgccggtttt gtaagaggta aaatgatctt gcgatagatt ccccactctg ataagccatc
     4261 gatccgcgca ctctcacata aagaatctgg gatactactg tagtattgct tcaacaagaa
     4321 cacaccaaaa gcattgaacg cttgtaatag caccaacgac cacaacgtat atgttaaacc
     4381 cacttttctc atcataatga actgcgggat catataagac tgccaaggga cagcaatcgt
     4441 gccgatatat gctaaaaata aaatatcgcg ccctttaaaa tttagtttag aaaacccgta
     4501 agcagcaaaa ctagaagtaa acaactgaat caacgtgatc acaacgctta aaaaagctgt
     4561 attcttaaaa aatgtcacta aaggaatacg agaccaaata accagataat tctctaaatg
     4621 ccaagtttta gggatccatt gaatcggaat cgtgaacact tcattatttg ctttgaatga
     4681 agcagaaacc atccataaaa aaggaatgat cgtcaataaa gctaatacaa tcagcagaat
     4741 catacccaac cattgaataa agggcatgtt ttttgaacga atttttgctt tttttctttc
     4801 atttcgaaca cctccaaagt tataatcttc atttctttgt aatgattaca atccagaaca
     4861 acggttatta tagctgattc atttccgttc ttcttatgtt tgtaagcgct tatttaacaa
     4921 gttatgtaaa tctctttatt ttttttgatt taaaaatgaa aaaatacaaa agcaagactg
     4981 tcatacgtct cgcttttgta ttgaaatttc ttcgattaga gatacttatt actgttcaaa
     5041 agcttctttg aaacggtgaa tcaattcatt gactacaggt gataaaatcc gattcttttt
     5101 ccagactagc atgcagttcg tttcaatcat tgggtcaagc ggaacgaatg tcactcgctt
     5161 tgaaagatga cttacagcac cttctatgac taaagcagca cctacctgat tctcaattag
     5221 cgcaaataca ttgaaaatca aattgtacgt tcctgtgatc agcaactgct ccttttttat
     5281 gcctaaccag tcagcaaaca ttcgctgtat ttctggacgc tttgagatca aaagaggtac
     5341 attcgcaaag tcttcttttg ttatcacgtc tttttttgct aaaacagaat tttctaaaat
     5401 gagcaatccc catttttcct ttcttggtaa caaaatcgtt tcgtatttct ctgtatctac
     5461 tggatctagc aaaatcgcta aatctaacag gcttttttct agtcactccg tgatatcatc
     5521 acttgttccg ctgtggatat gaaattgaac gctgggataa tcacggatca acttttctag
     5581 taatagtgcc aatgtattcg aattatcagc ttccacacag ccgatcgtaa atcggccgct
     5641 gaataattgt ttttttctgt tctcgaattc ttgagtggtt tgattcgtta aatctaaaat
     5701 ttcttctgct cggctttttt agaaaataac ctgcttctgt caagatcatt ttgcggtttt
     5761 cgcgtacaaa cagttctgtt cctagttcct cttctaactc cttcagttgt cggcttaagg
     5821 ttggttgtgt aagatgtaga acttctgcgg cttgagagat tgttccttct tctgcaattg
     5881 tccaaaaata ttttagtaag cgtaactcca tagcgatgct ccttgatttt tttacttgat
     5941 acattccatt ttatttctag ataacacacg atcatatttc ttgatttttc cttgtaaacg
     6001 ttccaaacat tcttgcattt cagcaatttt tttcgtcaaa agttcacttt cttcaatcaa
     6061 aagcttacgc cgctcaaata cagttgcttc tccttcgcta gataggcgaa tatattcaat
     6121 caaagactcg atcgataatc cggcatttcg catacagatc gtaaagtcga cccattttag
     6181 gtcttcttct gtataatcac ggattccgcc ttttgtccga ttaactggtg gaatcaaccc
     6241 gattcgttca taatagcgca atgtatcggc cgaaatgccc ttttcttcac tgacttgttt
     6301 gatattcatc tgatcactct ttctcttaat ttcttgccac tttacttatg tattttattt
     6361 attctacaac tttaagtgta ctccaagtca atactcattt ctattaaaac taagctggat
     6421 tatctatatc ggttgtttgt gtattatttc aaataaaaaa gtcttgtttt tttattcttt
     6481 tttggatata cttttaacaa tttagggaaa ttttaggaaa aggggtataa aaatgacaac
     6541 gtatcagcga aagttattat tatcgacatc ggcgggaatt gctttagaga atatggatat
     6601 tatgttttta gctttttcat tatcttcaat gattacttct tttcatatta gtggtacgca
     6661 agcgggtttc atttcaacga tcactaattt ggggatgctc attggaggta ttttttttgg
     6721 catcatagct gataaatatg gacgtgtgaa agtattttct caaacggtcc ttctgttttc
     6781 gattgcttct ttattgatgt attttgcttc aaacatctac ttggtttatc tctttcgctt
     6841 tatcgcaggt gtaggagcgg gaggagaata cggggcgtgt atgtccttga tttccgaaag
     6901 tttttcaaaa aaacaaattg gacgagcttc ttcggttgct ggtattggtg cgcaggtcgg
     6961 tgcggctttg gctgcgattt tagctgctgt gatcattcca acgtttggtt ggaagatgtt
     7021 gtatgtcgta ggtgtactgc cggtgttgat ggtcttgttc attcgtcgcg gcttgactga
     7081 accaaaggaa ttcaaatctg tacaatctgc cgaaaagaaa accaaactta gcagactgtt
     7141 tgaatcaaaa catcttgctt ggcaaacatg tggccttagt atcatggtaa ctgtccaaat
     7201 tgccggctat tttgggttaa tgaattggct gccttcaatt atgcaaaatc gattagggct
     7261 ttcggtttct gggtcttcat tgtggatggt cagtactatt ttaggcatgt ctttggggat
     7321 gatgactttt ggcataatca tggataaatt ggggcctaaa ctagcctttt ctttgttttt
     7381 aatttgttca tccttatctg tctatctact tgtgttagcc catagccagt ggagtttggt
     7441 agcagccgct gtgatagtcg gctactttat caatggcatg tatggtggat atggcgcagt
     7501 gattagtagt ttgtatccaa cagaaatccg cgcgacggcg aataatttca ttatgaacct
     7561 tggacgagct gttggtggtt tttcttctat cgtcatcggt ttcttgatgg atcattatag
     7621 tttaactgcc gtaatcttgt ttttgagcgg tatttaccta gtcagtctgc tggtcttgac
     7681 aactcttact ggtgttacag cattaaggga aagcttgcag cattgatagt tgagtctgta
     7741 aatgacaaga aagaggtgtg gcaaaagctt gcatctgcgg taataagacc agccaaatca
     7801 aaaacatgag acactgtttc cgtttggctg gtcgtattac ttgatgcagg gcagcttttt
     7861 caactgctaa tttccttcta tctggcggct actattcttt gacgtccgtc atttcaaagt
     7921 actttactaa taacttagca taacgaaaaa ctgtggatac actcgtatat tgagcgtctg
     7981 cataatcttc caaatttttc tcaggatgtt gatacatgaa ttcaatcaaa tgataattat
     8041 atgattttct gtagaaatga agataaataa tgtagattgg gaagttctcc tgcttttcca
     8101 tagaaaagcg ccgattattc tcattcttca aaatgacctt atcaggatat ttttggaaca
     8161 attcttctaa atctgttaga taattcaaga tagacttact tgataccttc aattcgtttt
     8221 gtagttcttc tattgaacac caacgttctg tttcaagcag taaatgaatc attttaattt
     8281 tcttttgaga aacttttgtt aaaaaagtct gcatattttc tcctcctgta tctactttac
     8341 tatactacat acaagaaaaa aaacggatac aaatatcata ttgtcacacc gtcaatagtg
     8401 cataaacctg ttattatttc tctttttttc ctaagtattc tattgatatg aatcatttaa
     8461 tcgtttatta tataaatgtc aataatctaa taataaaata acgtacatat tttattattg
     8521 catacttaat aacagaacta tagcaaaaaa tcccgtgtat caaaacggga ttagattaag
     8581 tttcctcaat cactattcat aacacaatta ttataacaag taagtaaaca attgaaaagg
     8641 acatttattg aaaacggttg ttttttactt aaggaggaaa tttagtgaaa aaattagttg
     8701 cattgttttt ggtcgcagga agtttgttga gtgggtgtgg tgctactacg tctactactc
     8761 aaagcacaag ttcagctcag gcttccagca gcagtaccac gatctccgaa agtacggcag
     8821 attcatcgaa agaacaagaa caaattgaaa agaaaaaaga agaagagaaa caaaaagcgg
     8881 aaaaagctgc actagaaaaa aaagtgacag aagcggaatc tgctttgtca caagctgaag
     8941 caaatcctac aaaagaaaac tatgcaactg cttcagcagc tatccaagcg ctacctgaaa
     9001 acaaacaaga gttgacaagc cgtcttgctg caattgattc cacaatcaaa gcaaaagaag
     9061 cagaagaaga acggaaaaag caagaagaaa ttgcacgaca acaagcagaa caagcgcaac
     9121 aagaagcaat cgcacagcaa caagcagaac aggcgcgaca gcaagcagca gctgaacagg
     9181 cctctcagca ggcacaagcc gcagctcctg agcaaaataa tgaacaaact gtctatgtca
     9241 caccgaccgg ttcaaaatac catacacata aatgtggaaa tggtacgtat acccctgcca
     9301 ctttaagcga agctcaagct cgtggtttaa ccccttgctc caaatgttat ggaaactaat
     9361 tcaaaagtta tactataata ataaaattct tctgaaacat aaaaaaatag aagctggcgt
     9421 tccagatagt aaaccaaaaa tagcagttta cacgtccatt tcgtcagctt ctctcttttt
     9481 tcatttagtc tgttccctct gcttttttgg acatcttcat tcattctttc tcaatcaaat
     9541 agtacacctc tttttctttt cgcagtttat atcttccttt tttcaatttc attgaaaact
     9601 gtttcgaatg tttatgtttt acatggattt taagcaggag ctcctcatgt tgcaagcgat
     9661 agttcctgcc ttttaaatca gaaaatctca ctagtccgta ttcatcagaa gctttcacaa
     9721 atggaacttt ttctctagta atatagtgtt tgccgttgag cgtttttaca gaaaagacca
     9781 ttccttcgat tggtcgtccg tttgtgttct ttacataaaa agataaagga tagtttcgct
     9841 tagcgatggc caaagaatct aaataagcga tgagtagcca aactaagccg attgatccaa
     9901 gtagtatacc gataaaccaa ataaaaaatt tccatttttt ccattgatca attccttgga
     9961 tcgctttttt tgcctctttt gcttgataag gaatacgatg ccccgtcaca agcagtcgat
    10021 gtgtattgac catataagga gtacaggtaa gcaaagtcac gagatctctg cctttttgga
    10081 tacccaaaga atctacttca tcaggtaaga ccacttggat tttttctact tgatacgcca
    10141 atgtctttcc attgatctgg atgaaaaact ggtctccttt tttcaactta ggcaaatctg
    10201 taaataattt tgcttctggg agtccacgat gcccagaaag aacggcatgg gtgtttttcc
    10261 cgccagtcgg ataagaactt tcttccaata atgacgttcc tttttgcaaa aatatttccg
    10321 ttgtctgatc gaatataggt aagctgacat tgatttttgg gatttcgatg actccaagca
    10381 tatgttccat aaaaaaggca ttactaggta aatcttttaa tgcttttgca tcaactgctt
    10441 ggttgaaact gcctatccct ggtgatacgt tttgctctgc caactgttgg tttttctttg
    10501 tcattttttc ctgctgtttt tttatttcct ctgaattctt attcgatgct tgcttttgat
    10561 aatggatgat catttgctgg gcaagaaaat cattcagtga atcttctaca aaaggataag
    10621 aaaagatccc gatcccaacg actaaaatga ttgccatgaa gatgtccagt attcttattc
    10681 tgcttttatt catcatgtcc acctattttc taatatgtat atgaagaggt tgtgaaatca
    10741 gcgccttgac ctgagtattc gctcaacaaa cggcaaaata gttcctccta ttttgccgtt
    10801 tgttgagcga ataccaagag accgaccact tgtcacaacc tttctcccca tttgaatcac
    10861 tatcgtttat ttatcgtatg atttttgttt ccaatagttt aagcttctgt ttcttgatgg
    10921 cgttttttga agtagatcac tgctcctcca actaaagcag tacctaccaa gatgaatacg
    10981 atgatcccca taccaccagt tgaaggcaag attcctttag gttggttatc gatcgttgtt
    11041 tcatcagaag ttgttggatc atcatctact gtaaattcag tgtacgcagc ttcattctta
    11101 ggtaatgcgt agccttctgg tgcttttgtt tcttgaaggc agtatgcccc ttgtttcagc
    11161 ccttcgattg aaaatttccc ttctgaatca gaaatcaatt tcacttcttc ccagtcagaa
    11221 cctgtgactt catctgacca agcatatggg tattctcctg aggcaagatt tgctattttt
    11281 ttcattaatt taacagttgg atcttttgct cttagaatag aaaattctgc atcggctaat
    11341 ttttcttgcg tgttcacatc ttgtttgatg aagtggaaat caccgtagtt ttggacattt
    11401 tctacagtaa tcgtgttatc cgtagttgta ttttcccgat tgactacacc gtttggattc
    11461 ccaagattgg cttcatctgc gccatggatg ataccggttg tttgttgcaa ttttccgttt
    11521 tttactataa aagtagtttc tttttgtgga ttggttaatt gataaccatc tggtgctttt
    11581 gtttcaacta aagtataatt gccgtctatt aatccattga cactgatgga tccttcagta
    11641 tcagttgtga atgtcaacgc tgactcagca ggatcataga gagcgacacc agtgttttca
    11701 tcaaaaccgg tcacgaattg attctctgaa tttttgatct ggaattgcgc ccctgctaac
    11761 ttatctaccc ctgaaaagtt cccagctttc tggattttga tattgctttc tttgatgaca
    11821 tttttagcat ataggtgaat cgtgtctaaa ggtttgtcag ggtctgatga attcgggtta
    11881 taaaagggta agcttaacac gatatttggc gacgtttcga caccgggctt tggtgtttct
    11941 acaaagacgt atacagcatc tttcccatca gatttacctg gaagagtcag gttagctaat
    12001 ccttcaccag aagtcgttac acttgcgaag ggtgtagcgt ctgctggaac tgctgtgtct
    12061 ttacttggat agtacgtagc agtgatgtca tatacgttga attcaacgcc gttcaacggt
    12121 tcgctattcg gaaatgtcaa ttccgttccg ttgtttattt gattttctgg tgctgaagtg
    12181 aatttcaatt tatgcaaatt aaccgtaaca ttttctggtg taggtgatac ggcctagaaa
    12241 cctctgcagt tcctaggccg agaacgaaag gaagcagcat gatagcagtt cctaaaaacg
    12301 tccatatttt tgttttgatg tttttcattt tatttccctc ctattgtttc ctttttgttg
    12361 ttttttataa aaccaagctg tccctgttga aaggactagc aacaacccta aaattgagaa
    12421 acctccaagc atctcttcgc ttgtttttgg aaagcttcct ccattttttt catttgatgg
    12481 accatttgtc tgcatctgaa ctggcgtttt tccattattc gtcggatttg cttttggttc
    12541 gttgatgatt tccaatggta taggatgaag cggatcataa ctgccttttt caacttcaaa
    12601 gagaatggtt gattgactca atatatatcc tttaggcggt ttaatctcca ctagttcgta
    12661 acttccaaaa gacaatccat ggacagaaaa tgttccatct ttactggaag taaaaatcgt
    12721 tgctcttgaa tcatctttgg ctacccaatg atttatcccg ttttcttgcg ctaagtattg
    12781 gtcatttttt ttgactagaa attgagcatc tgctagtttt tgtttcgttt gccgatccac
    12841 tttgacaaat tgtttgcctc ctgtttggat ggcaagatgt gtttctactg tttgattgtc
    12901 agtcgtcaaa cgtacatcgt tatcaaatgt cgttttttca ctgttttctt tcaatgtcat
    12961 ttggtaacgg atcgttaatt ttttcccagc aaacgaacta agatcttttc cagaatctga
    13021 taagacaaaa ccatgttttg ttttttgtgt gtgaaatgta tgaatcttgt ctccaccaat
    13081 caaaaaatcg atcgtttctg gttctagcca taactgagga tcagctgtat ctttgatcgt
    13141 ataggttcct atctcgttga tgttctcagg aattgttacg gtgatgagat aaggaacttt
    13201 atccccatct gcaaaatctg tctgcttatt ctctactgtt tttgttaatg tgagtgcagc
    13261 aggtattttt tgttcgctct ttggatacaa gtggatggtc gtcaatttat gattagatga
    13321 atctgtcaca ggaagcacaa cgacaaatgg tgctgatttt tcttttactt aatctgaagt
    13381 ctttatttcc gcaaatcggt aaacagcgtc acgaccttgc tcatctttgt ccgatgcact
    13441 aaaagaagca atgccctctt cctcgtctac tgtttttgtc acattttctg ccagtatttt
    13501 ttcactatca cttttttgtg ctagttttct ttgtgcgtct tccacactac ttccttctga
    13561 ccgcagcttg taaaattcat ccgagacatc atatatagcg aaagtaacgc cgttcaaccc
    13621 ttttactcct tgaaagttat ccgggatatt tattccgtcg ttttgactga tacttggcat
    13681 cttatcgctt tcgaacattc gattatgcaa agtaaatgta ataggtgatt ctctagctga
    13741 cacagtcgga gaacctataa aaaagaccag taggataaaa aagagaacag caaatatatc
    13801 cacacgtaca ttttttgttt ttttcatcgc tgttcacctg cttttttctt acgatggata
    13861 aaataaaaga agccagaaaa aataaagatc agtcctaaaa agacaaaggg cagtagacct
    13921 atcccacctg tcgaaggaag caagagagtc ggattgatcg tcaacttagc ggtatctgtt
    13981 gcgataatcg tataattatt gtttacactt ccttcgccta aactaatcgt tactatttgt
    14041 gatccaatat ctgtccggct gttaactgtt agggtataat ccaatgattc gcctaagaca
    14101 acaccggtaa cgtggagatt tccaaccgaa atcgagtgac tttgggcatc ataattaaag
    14161 gcagcgtcat cgacagtaat cactgcgtta gctttataga tggtataagt tcctgatgtg
    14221 ttggctatat cagtaaatga caacttggga tttgctgctt tgactttgtc tattccgcta
    14281 ttcgttagtt taattgtata gctgccagca ttaaccatat tttttgctga ttgtgcatct
    14341 gctgtgtaat ccatactgct aagtgccact tgtactggag taagcgaact agtgacaccc
    14401 tctgctagct tgacatggat tgacgggaca cctgcttttc catcatatgt cttactgcca
    14461 ctgagctcta ccgcaccgct gatttgctta atcgtataga catcagatgt ctgcacgata
    14521 gtaaataaat agtttgttct ggcagctagt ttagctagtc cgctttttgt caattgccaa
    14581 gacgtatagg ttccaatttc agtaggcgca gttacttcaa ctcccttttc attcatcgct
    14641 ttgaaatcag ttggattcca tgtgatttga tcaatactga gatcattgtt taaaagggat
    14701 gttgattctg ttttatcatt aaaggcatat ttaacgatcg gtacaacaga aagaccatca
    14761 tagtacttac tgccatttgc attgattgat actgttgcat ttgtcttata atataggatg
    14821 acttcagaaa tctgttttcc atttgcgtcc tttacgatta catttccatt tgtatctaaa
    14881 ctagctaaaa cactcattgc tgattggttc tttgtttctg tcgctgaaac agctgtaaag
    14941 gtatatccgt tgatagtagg cgtattagtc gtcaggtctt gtgcagcact tccgtaagta
    15001 agattcatta ttttagaagg agcaatcatc tgtccaaaat agtcttgata cttaatagtg
    15061 attggtgttt gtttgcctgt ataggtcacc gtatcgacgt tactttctag ggctgtgaaa
    15121 gaaccgtttg tttgtgcaaa agaatagccc gttactgctg ttggagtgat aaccactttg
    15181 tcacccacat ggattccaga taatgtagtc ccgtacgttc cgatttttgc tgctccacca
    15241 ttgactgtat aagaaaaagt tcctgtccga ttcgcattga cctgaaccgt tgctgcttga
    15301 taggttggaa aatttgaaac atttccctta ccatcaacga tctgcccatt tttgatcgag
    15361 taccccgtga atggaatatc atttgctaac tgtataccga ttgaaaaagt actcatacct
    15421 cctccggcag gaagccctcc ccatgttgag ggtgtaaaag aataactttt atccgcattg
    15481 ggtgtaactg aatcgatcgt atttccagcg gtagccaccc ccatatagat actttttggg
    15541 aatgtgatag tagtaccatt tactgtggag ccaataaaca gattatggat atctggacgg
    15601 ttatcaataa aagcgattgc tttggttacg ccttgtaacg cagttttatc cgcactcatc
    15661 gatccgttaa ccgcaacaat gactccatct ttggcatata tcactgtata tccatctggt
    15721 atggtatgtc cgttgaaatt cgcagtggcc gcaacagtac taaatgtctg gcttctacct
    15781 gtttctgaat aagctttctc ccctgctgct tttgctgctg tgatttcttt ttctgtcgga
    15841 tcaatcagtt gagtcgagga tgtggttgag gttgtctcgt tgttgctaga ctgattggtg
    15901 ctttctatgg actgtatttc agaatagggt ggactatttg tctcctctgt cgtttggtta
    15961 gcaaaatttg acgtttctgt ataaacgttt acataaatac ttgataagct ccctaagaaa
    16021 gtaaaaatca agatgatcga aatcataatg ggatgtaagt attttctttt agatacagct
    16081 ttgagtctag aaacaaaatg tttttgacat atacgttgga ttttattttg agttttctta
    16141 atcattttca tccctcctct caataaaaaa tcatcaaata atcctttaca cgttattcta
    16201 aat
//
LOCUS       DACIRY010000054        16122 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.055, whole genome shotgun sequence.
ACCESSION   DACIRY010000054 DACIRY010000000
VERSION     DACIRY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.055"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(103..1521)
                     /gene="nhaC"
                     /locus_tag="IYE22_001544"
     CDS             complement(103..1521)
                     /gene="nhaC"
                     /locus_tag="IYE22_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ9506758.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1925..3148)
                     /locus_tag="IYE22_001545"
     CDS             complement(1925..3148)
                     /locus_tag="IYE22_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ9506759.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3180..4019)
                     /locus_tag="IYE22_001546"
     CDS             complement(3180..4019)
                     /locus_tag="IYE22_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ9506760.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4036..4716)
                     /locus_tag="IYE22_001547"
     CDS             complement(4036..4716)
                     /locus_tag="IYE22_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506761.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(4718..5791)
                     /locus_tag="IYE22_001548"
     CDS             complement(4718..5791)
                     /locus_tag="IYE22_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9506762.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(6441..7112)
                     /locus_tag="IYE22_001549"
     CDS             complement(6441..7112)
                     /locus_tag="IYE22_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ9506763.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(7200..9878)
                     /gene="mgtA"
                     /locus_tag="IYE22_001550"
     CDS             complement(7200..9878)
                     /gene="mgtA"
                     /locus_tag="IYE22_001550"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ9506764.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWFFITVVAYLALVTAVKKIYIRRFQELL"
     gene            10538..10975
                     /gene="ndk"
                     /locus_tag="IYE22_001551"
     CDS             10538..10975
                     /gene="ndk"
                     /locus_tag="IYE22_001551"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ9506765.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(10997..11863)
                     /locus_tag="IYE22_001552"
     CDS             complement(10997..11863)
                     /locus_tag="IYE22_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9506766.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(12097..14175)
                     /locus_tag="IYE22_001553"
     CDS             complement(12097..14175)
                     /locus_tag="IYE22_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ9506767.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            14668..15885
                     /locus_tag="IYE22_001554"
     CDS             14668..15885
                     /locus_tag="IYE22_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ9506768.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
BASE COUNT         4707 a         3475 c         2785 g         5155 t
ORIGIN      
        1 ttcgtttggt taggcttatt accgcaaatg caagcttttg aacaccgttt attcgtaaaa
       61 gggagtgtga caaaagtctt gtcacactcc ctttttacgt ttttattgtt ctaattgcgc
      121 tgtcttgctt actgcttctt gcggtttcac aaatttcttt gagatccccc aacctgtaaa
      181 ggcactgacc atcgaaaaca caggtgaaag caaactgaaa aacacgaatg gcaagtaagt
      241 gagtgtagga atgccaaatg tacttgctac gaaacttccg gcgattcccc aaggcactaa
      301 gtagttgatt actgttccac catcttctag tgttctccct aatacagttg gatcaaatcc
      361 tgctttttcg taagtttctt taaatgcatt accaggcagg ataacagata gaaattgttc
      421 tccaataaag atatttactc cgatgcttgt catcaatgtt gtaaaaatca attttggggt
      481 gttcgtcatc tttcttgata ctcgttccat aacggttgag atcaacccaa attcgatcaa
      541 caaaccacct aatgacaatg ttagaataat caatgacaca gttggcatca tgctttcgat
      601 tcctccacgg ctaagtagtg aatcgatttg ttggtttcca gtatttgaaa caaatcccga
      661 ttcgatgacc cctgccaact tcgttaagga agtttgtggg ttttgaataa agatcattcc
      721 tactgctaca gcaatattca acaaaatcgt caaaattgcc ggtacctttt tccatgcaca
      781 caagagcatt aaaaatagag gaatcagtga ccagatcgaa atagtaaagt ttgcttctaa
      841 aatatcagtc acttcacgaa tagatgaaag acttgcggct ttacttgtgt gtcctaaaat
      901 catgaataaa aagaaagaaa caacaaatgc cggaacagtt gaccacatca tatgcttgat
      961 gtgtttgaac aaatcggcat caacaatagc tgctgctaaa tttgttgatt cggataatgg
     1021 agacatcttg tctccaaaaa cggcacctga aacaatggct ccaacaacaa gggctggatt
     1081 tagtcctagt gttgtcccaa taccgaaaaa tgcaatccca atcgttgaca taacagtaaa
     1141 ggcactccct acagcgctac ctacgattgc acaaacaaca aatacagaag ggacgaacca
     1201 ctggacacta atcaatttaa atccaatgac catgagggtt ggaataattc ctgcttgtat
     1261 ccatacagca atcaatgaac cgactaaaat aaaaataaag attggaatga tccccggttt
     1321 gatgccattg atgatccctt cgtgcagcca atctgtcggg tattttttta tcactgcgta
     1381 caacatgacc attccgatag ctgccaaaat cgtgatatta ggtgaaacct ttaatccgac
     1441 aacgccaatc gcaatgccta aaacaatgac catcaataca agtgttgctt ctaaaaaact
     1501 tggtgttcct tttttttcca tgataatctc tcctttttta gttgttctta tgttctaaaa
     1561 aagttaagat atcggattac tccgccacaa ttcatccgcg attcctcctt tataaaaata
     1621 aaaagacctg ctttcaacta aggacgaatg actccgcggt accaccttaa ttaaaaacag
     1681 atctgttttt cactttcaaa cgattattca aatgtatgga ctttccatgt aattcgtcaa
     1741 ttaggcttga tcggttcaca gcgaccaccg actttctgaa caaaacacct aaccactact
     1801 gcgtgaaaag ttgacttgta tgaagtttac cttccataag atcatttgtc aatgaaaaag
     1861 aaaatatttt ttcatttttc tgaaaattat ttttataact ttcgcgcaca actttatctt
     1921 tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgtt
     1981 tgattttttc taatagcttg ggttctctca ataaatctag agctgtattg gcaagtaatt
     2041 ccgctgcaat cgcaatagaa gcaagtcctt tttcactttt agcagcagct ttgaattctt
     2101 ctgtatgtcc ggcaatatat gtctcagaga ttgctacagt aggctgtatc gttggaataa
     2161 cttggctaac atttcctaca tcagaagaac ctagacttgt tctttgttcc gtttctattt
     2221 cttcatcagg aacccctgct tcttttgcat gttggaagaa taagtcatcg aaagaaggcg
     2281 ttacgatgac atcatcgact gcattttgga acaatccaaa ctcaaaagtc gtacccgtgc
     2341 ttaatgcagc acctcgtacg atattttcca ctttttcata tactttgtct aatgtttgtc
     2401 gatttcctgc acgtaaatag aaacgtccag cagcgtagtc tggtatgaca ttggccgcca
     2461 cgcctccatc tgtaatgatt ccatggatat tgacatcttt tggcaattgc aagcgtagag
     2521 cgttgattcc gttaaacact tggatcaagg cttccaaagc atttactcct ttttcaggtg
     2581 ctgctgctgc atgagaagcg acaccgtaaa atttgatatc gactggatca tttgctaaag
     2641 attctgtcgt tttcccataa cgaaaagctg gatggacaca taatgctgca tcgacatcct
     2701 caaaaaaacc ttcccgaacg aagcttccct tagctgatcc attttcgcct ccttcttccc
     2761 ccggagtacc atacacacga atttctccgc caatctcatc aatgaattgt ttcactatgc
     2821 tggctgctaa tacggagtaa gtaccaaata aattatgtcc gcatgcatga ccaatacctg
     2881 gtaaagcatc aaattctgcc aaaaaggcaa ttgtcggacc tggtttattg gaaatataac
     2941 gtgcatcaaa gcctgttcgg tgcccagcta cttctttttc tacccagaac ccttcttttt
     3001 ccaattgttt gattaaagct gctgaggaat aaaattcata attacttact tcaggatggt
     3061 cgtggatagc tagtgcaagt gcttgatatt ctggtaaacg gtattgaatc agatccttca
     3121 attgatttgt tgagagtgtg gttgtcataa aaatttcctc ctatgctagt cgttttttct
     3181 tattttctcc cgaatgtttc ccacgcaggt acgcttgaac cttttgatgt tttttcgatt
     3241 actttggctg tttcttctgt ttgataggca tctactattt tttgatacgt tttattgtct
     3301 tcatcttctt ttcttgcaac gatgatattg acataaggac gtgcatgctc atcgactggt
     3361 tctaagaaga ttgcatcaga agcgggatta aatccagcat ctacagctac accgctattg
     3421 atgactgaaa tatctacatc tgacaaagca cgtgctgttt gggaagcatc taactcagaa
     3481 atgtctagat tttttttatt ctcggtaata tcagaaacgg taggcgcttg gccttttgcc
     3541 ttgtccactt tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccattcgtg
     3601 acatcattag ggattgcaac ttttgctcca tctttgatat cagccacatc ttttaacgta
     3661 ttggaataaa ttcccaacgg tgcattaacc gtattcccaa tcgatactaa atcagttccg
     3721 tgttcttcat tgtaattgtc taaaaagaat tgatgttgga aggaattcaa atcgatcgat
     3781 ccatctgcta aagccgcatt tggctgattg taatctgtaa atttcacata ttccaaatgg
     3841 atgttttcct ttttcaaacg gtctttcaca tcgtcccata catctgtatc ttccccaatg
     3901 atccctagtt ttacggtcgt ttcttttttt tcagttgtag ctgcttttcc ttggctgcat
     3961 cctgccaacc aaataattgc tacaaatgta atgactgcaa aacgaataat ttttttcatt
     4021 ttttctcctc cattttcaat gtgtgacttt tttaatcagt tgattactga taaattgact
     4081 caaaaaaact aagaccagaa tcaataatgt agcgacaatc gttacatctg tttggaatcg
     4141 attatatcct ctcgatatag caagattccc taatcctccg gcacctactg ctccagccat
     4201 ggtagtcagt ccgatcacat tgatgatcgt caacgctgag acccgaatga taccaggtaa
     4261 cccttctaac aaataaacac gtaaaatgat gccgattggg ctcgtgccca tcgcttctgc
     4321 tgcttcgatc accccaggat caacttctag cagtgcattc tcgatttgac gggcaaaaaa
     4381 cgggacaacg ccaaaaatca aagggactaa agctgctttg gcaccaatcg tcgttccaac
     4441 taagaaacga gtagtcaaag ctaaaagaga aagcaagata atgaaaggga tggaacgaat
     4501 gatattgatc aacttatcaa gtatttcgaa cagcagcctg ttagctagta atccatttgg
     4561 acgtgtcact actaacgtca cacctaagat gattccgatg atcccaccaa tgaatgccgt
     4621 ccaaaaagac atatatagtg tttgctttgt cgcttcgatg aattcatccg gaatcaaata
     4681 gacattcggc aaatatgttt taaaaaattc agtcatttta gtttcctcct tcgatgactt
     4741 tgatacggtt atctaattca tttttttgta aaatagttac ttttacacca gatttaatca
     4801 aataatccaa tgctttttgc cgctgtttgg tttctccagc taaagtaacg atcaaacttc
     4861 ctaagggtgt ttcttgtaat agctctacat taccgtataa gatattcgct gttacttggt
     4921 acttgctgta aagatgagcg attagaggtt cgttcgtctg gttgccgata taacttaatt
     4981 ccaccaacca tttatttgca atctgatcgg caaaagcagc atgggcactg attgtttcca
     5041 atgcttggtc aaggtgtgtc gctgtccgaa taaaatcttt agtcaattct tcttcaggat
     5101 gactgaaaat ctggatgctg ctaccttttt caatgatttt ccccgcttcc ataaccgcta
     5161 ccttgttgca gatttctttg acaacttgca tctcatgagt gatcaggacg attgttagac
     5221 ctagctgacg atttagtttt tttaacagtg caaggatctg aagggtcgtt ttcggatcaa
     5281 gtgcactagt tgcttcatcg cataacaaca ctttaggttc atttgccaga gctcgtgcga
     5341 ttgccactcg ttgtttttgc ccccccgaca gttgtttagg atacgcagat gctttttctt
     5401 ccagaccgac aagttccagt agttcattca ctttttgacg acgttcctgt tttgattttc
     5461 cagaatactt tagagaaaaa tctacgttgt caaaaatcgt tcgactttcc attagattga
     5521 aatgctgaaa gatcataccg atggcctttc tttcttttcg caattctttt ggagagagct
     5581 ggcttaaatt cgtctgattg acgatgactt ctcctgctgt tggtttctgc aacaaattca
     5641 tcacgcggac taacgtactc tttcctgcac ctgagtaacc gacgatccca taaacatccc
     5701 ctttttcaat cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga
     5761 aacatacaga aatatctcgc aattcaatca ttaacattcc tccttgtcta taaaaaaagc
     5821 atcctcaaga aaatgaaaat agtcattttc ttgaggacgc ctttttgcgt gttaccacct
     5881 catgttcaag ctgtgttcac acacaactcc tcttcaagta ccatcatact ctagcaagat
     5941 atcggtcgca gccgtcaccg ccttacatct atgctcggga tgaattgctc gatggccatt
     6001 ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa aggttcttct
     6061 atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aaatcctttt actaacataa
     6121 actatgtcag taaaaggacg acgtatcgtg gtaccacctt ttttcggaag tttccttccc
     6181 tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga acatttccga
     6241 atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc ttacttgact
     6301 cttcacacca gccagagcct ctctgcaaag tttccaagct tactttcctt ctcaacgaaa
     6361 tcaatatttt gttgttggga ataatctaca ggaggaaaac tgaaaagtca agcatgaaat
     6421 ccaaattatt ttttctatat ttatttttct gtttttttat ctaacatttc aataacagtt
     6481 cgaagatctt ttccttccgc aagaaggttc aatttttcct tctcttcgac gctataattt
     6541 cgaacaaaac tggaatcggg aatcaaaaca acgcctagtc ctgcacgtga agcggctgtc
     6601 gctcctacta aagaatcttc cactgcaaaa gctgaaagag cttctaatcc tgttcgttcc
     6661 aaagctgtca gataagggac tggtgaaggc ttgttctccg atacttcatc tccgaacgta
     6721 aacgtatcaa agtaatcggc taattgaaag tgatccaaga tatctgttgc tcttttcgta
     6781 acagttgtcg tagcaatccc tagtttcata tgacgttctt ttaagtctgt caatatttct
     6841 tttgcatagg gtttcaattg gatagctcca cttgtcagct gttgataaaa atattcttca
     6901 cgctttgctc ggacttcctg cactttttca ggactgcctg ctgcctgcac taaaatatca
     6961 aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttctttcgg gatatgaata
     7021 tcaaattgct ggaatcccca aagccaacca tcccgatacg ttctttccgt atccactaac
     7081 aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc caaaacaata
     7141 cacctagttt agcatgactg atccaaaatg aacagaagca tagagcccag ccgcatcact
     7201 tataagagtt cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga
     7261 taagcgacga ctgttataaa aaaccataac cagtaattgc cagggagcgg catcaagcct
     7321 aactcagcac caaatcctgt gaatggaagg actgaaccga caatgattcc taatgtagtg
     7381 ataaccagca tggcaaatgt tgcattgctt tggataaatg gtattttcgg tgtacgcaat
     7441 gcatggagta ctaatgtttg tgtccacagg gattcgacaa accatcctgc atggaataat
     7501 gcgataaaaa tcacttgttg tgagctatcc aatgtatgga aacttccacc aactacagca
     7561 ggacaaataa caaaatacat caacaagtaa gtagtgatat caaaaatcga acttgttgga
     7621 ccaaaataaa ccatgaattt tccaatacta gatgcattcc atttctttgg ctcatccaaa
     7681 tattctttat ccatccgatc ccatggtaag gaaatgcaag aaacgtcata gatcagattc
     7741 aaaaatagaa gttgcaaagg caacattggt aaaaatggta agaacgtact ggcaatcagt
     7801 acggaaaaca tattgccaaa attagaactt gctgttgcct tgatatattt catgatgttt
     7861 ccaaaagttt ctcttccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa
     7921 aggatcacgt ctgctgattc ttttgcgata tccacagctg tatccacgga aataccgata
     7981 tcagccactt tcattgctgg agcatcattg ataccatctc ctaaaaatcc gacagtatgc
     8041 ccatttttac gtaaagctgt cgtcaatcgt gctttttgct gaggattcaa ttttacgaat
     8101 acttcgtgtt cttcagctac ttgcgctaat tcatgatcat tcatttccga gattttctca
     8161 ccagtaatca attcattgat ttctaggcct acttgacggc acacagaccg tgtgactaat
     8221 tcattgtccc cagtcagaac ttttacagct acaccatgtt cttttaaagc tttcaacgct
     8281 tgttttgttg tttcttttgg tggatcaagg aaagcaagat aaccaatcag aaccatgtct
     8341 gactcatctt tgactgaaaa ttctccaaca acgcttggat tggtcttttg tgcgacacca
     8401 atcactcgta agccgtcttc attaagtgat tgtacttttg caagcaccgc ttcttttcgt
     8461 tcttttgtta atggtgcgac agtaccacca agatcaataa atttagaaat gttcaacatt
     8521 tcttcaatcg cgccttttgt gatcatctgt gttttgccag ccggatcagc gactacaaca
     8581 ctcatacggc gacgttcaaa atcaaaagga atttcatcta ctttttgata attgattgta
     8641 tctgtatcta aagtttctgc tgcttcatca atgatagcca catctaacaa atttttcagc
     8701 cctgtttgat agtagctgtt tagataagca tggcgcaaaa cacgatcatc ttcatttcct
     8761 tcacaatcca tatgatattc aagaatgatt ttgtcttgtg tcaatgttcc cgttttatcc
     8821 gtacataaca catcgatcgc accaaaattc tggatagcat ttaaattttt gatgactgtt
     8881 ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc
     8941 atttcaggcg ttaaaccaac tgccacagat aatccaaaaa gaaaagcttc taaccaatct
     9001 ccttttgtaa gcccattgat aacgataact gctggagcca tcaatgacat aaatttgatc
     9061 agcagcatgg atgttttgtg gatgcctaat tcaaaacttg attggatggg tttttcagat
     9121 agcgtttctg ctacatgccc aaatagtgtg tcatttccga cagaaacaat gattccttcc
     9181 gcgcttccac tgatgacatt gcttcccata aagacaagat tttcataact tgtttcactt
     9241 tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat
     9301 gctgcttgag aaatgaatag atcctttgcg gacaataaac gcatatccgc tggtatcata
     9361 tctcctgctg aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgta
     9421 tactcgccat ttcgtttgac agctgccgtt actttcacaa gtgatttcag tttttcagct
     9481 gcttgattag aacgcacaga ctggatcaac gtcatcgtcc cactgacgat aaccatagtg
     9541 aagataatgg ctacaccaaa gagatcttta tctcctggag cggctaatac gtattccgta
     9601 atgaatgaaa taattcctaa tgcaatcaac actaatgtaa agggtgtgat gtatgctttc
     9661 aatatttcta taatgaatgg tgtttttttg ccatatgaaa tcgcattttc accataagtt
     9721 tctcttgaac gctcgatttg ttcttctgac aatccatttg gtgatgcatg gaacaattca
     9781 aataattctt gcttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt
     9841 ttttccatct cgatctctcc tttgtgtcca aaagacagct gttctcgctg tttttggtct
     9901 tttgctattc atcggatcga tagagaaaat gctgaaaggg gctgagaaca gatagataat
     9961 agccctggcc tttcctctaa atccttgttt ttcgaatgac tgtcatccat ttgttctcat
    10021 ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata gttattttcc
    10081 caactcgctc acctcccttt ttgttttttc acctatggga tttgcggcaa caaaaaaacc
    10141 tctcgccaaa tactcagcga aaggtttgaa aaagcaaatc aaaaaaagct tctcagatct
    10201 catcgttgag ttttgacact gtacaacgta aaaagcagat atctgctttt agctacctta
    10261 tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg
    10321 cagtagccta tgtttgtata ggagcctcac ctaacaaatc ctattcggtt ttccctcgtt
    10381 aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tactttttta
    10441 aaatacacgc gttatatttc ctgtctcacg cagttttatt tgtctcattt tccacctccc
    10501 gctataatag aattatagta ggaaacgagg tgctgttatg gaacgaacac ttgttattat
    10561 caagccagac ggcgtaagac gccatttggt cggaagcatc atccaacgtt ttgaagcaaa
    10621 gggactggcc attgctgaaa tgaaatttgc aacgatgaca cctgaactag caaaagaaca
    10681 ttatcagcac ttgactgaac gtagtttttt tgatgagctt atcgattaca tgacttctgg
    10741 tccggtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt
    10801 cggagcaaca aaaggggcgg atgccgttcc aggtacgatc cgaggagatt atgcactgcc
    10861 ggggactgaa aacatcatcc atgcttctga ttcccgtgat gctgcaacaa aagagatcgc
    10921 ccgctttttc ccgccattcg atacaaacaa accgtctgaa aaagcggcga agtgatacaa
    10981 atcctaaact gttggtctaa ctgctgggtt tagcaatggc cagcagtttt ttctctaaag
    11041 aattcctttg attcttccct aatgtatatt cgatcactgc gaaaaactct tggtgatttt
    11101 ccgatttttc ttcccctaaa gcagcagaaa tcagtcgttg gtaataatcc gccatcaatg
    11161 tttcataaat cactgtactt ctttctttat gcgctaaact ccgtttcata tcttcattca
    11221 cgctacgtgc cttttttaag cgaccatttt ctatataaaa caaagtcaag ttcgtcaaaa
    11281 aagtaaagag atactgctcg tgattattca gttgcgcgat ccgtcgcatt ctttttttga
    11341 ttttcaaata attattctca ataaaacgat cactgaatat ccaaggaaaa tctgctagaa
    11401 tagatacttc aaattgccca tactctggta cttgcatcaa atattcttca atgatacgac
    11461 attcataacg caaaaattca aattccgtct cagatacttc tgtttttttg gacatgtgca
    11521 gattcaaaac caaatagtac agatcttctg ttttcttata gtgcctgcgg ttattagcaa
    11581 tttctttgta aggatcaaag ctgtcttctt gaatccgatc tagcattcgt ctttttttct
    11641 ttaatttttt tggctcatca agctgatttc gtatccattc atattcggca agtgtcatgt
    11701 tcattcgctc taaaaatgat ttcaatgttc caaacgatac ttttttcact gaatttgtct
    11761 cgatcccgac tagcgaagtt ttagaacaga ttccttccgc taattgggtc tgtgtcagct
    11821 ttttgttgac tcgaagcttc ttaatcaatt ctccatcata cataatagtt ctcctttttt
    11881 aattgaaatt aacccttggc ttttccagaa tgcttgccaa taagaggaat gattatttca
    11941 atttaaattt tccgcattca ataaatagat acgttaaaaa taaaaaatac acgaaaaaaa
    12001 taaaaaaatg tttttctcat aaaaaatacc aaccttcaag catgagactg ctgtctaatc
    12061 cctgaaggtt ggtttgattg tttattgatc atttttttac tgctccgcat tggttcctgt
    12121 tactggatat aggatagtcg ttgttttatt gccgtttttt gaaaagacac tggtcgaatg
    12181 ttcacctttt tgcttttcga ttgcttcatt acgttccgct tcgtttttat agtcatattt
    12241 tttcggatca atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt
    12301 cagcttatct gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc
    12361 gtcgattcct gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc
    12421 tcctaggacg gtgtatttgg gcgtcacgaa gttcccattt cgaaaagcaa ctacttgatc
    12481 gtgctgagaa gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa
    12541 tagatgtagt aatgtcggca aaacatcgat ttctccacca tattcatgat tgactgtccc
    12601 tttagtgtaa ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa
    12661 ttcatcccat gtatctggat ctttaccaag agcacttgct aaatctttat tatctgtatt
    12721 cgagatccca aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt
    12781 tttcaaatac gtaaaaaatt gttccaatga ttgatccaga taatgggccg ttcgaacata
    12841 attatctact gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt
    12901 ataataaggc gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg
    12961 ttcaagatat tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt
    13021 ttcatcggat tcatcaaaat aagatcgatc aaagaaattg tcatacccta aatttttata
    13081 tacatgatct ctattccaga aactagctac attcccatgg aaaacagcac ttgtatagcc
    13141 ttctgtttga ccaagggtcg ctggtgctgc ttgaaagaca ttatctgacc caagttctgt
    13201 gaataatgag ccttgaggta aaccaaatgt gccagtttcc aacatgtttt cagcatcact
    13261 tgtctttccc tgtcctactt catgaaagaa attgtcaaaa ctgatcgttg cttgattctg
    13321 gaaaatacta tttaaaaacg gcgtgacttc ttgtccgtct accttcatat tgatcaaaaa
    13381 ctgttgaaaa ctttctaaat gaaggacaat cacatttttt cctttagcaa tcccaaatgt
    13441 ttcaggattt ggctctgcat aatgtttctt tgtatagtct aaaacttcat caattccatt
    13501 actgcttgca tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt
    13561 gtacgcatcg attcctagat atttgacaat ataggaacgg tcaaatgtcc gctgtagcaa
    13621 ttggggacgg ttggcttcgc tcaacgctaa atttcctgaa aaaatcaatc ctgaaagaca
    13681 ggtaacagcg atagccatcg gtttgctgac agggttgctt ttgataggta ttttcttgac
    13741 cttcaaccaa accagtagcc caatgaacac agcgatatcc aaccaataga tgacatcatg
    13801 gggtttcatc aaagaaaagg agcttcctga caacccttgt gacactttcg aaaagcctaa
    13861 tacagatttg actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa
    13921 caaaagcgta tttaaaatat caatgatcaa aagaatggct aacgctggct ttggcttttt
    13981 aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca atcgggttga tccacaagat
    14041 aaagtactgg atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt
    14101 cttcagccag aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt
    14161 tttaagttgt gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg
    14221 aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat
    14281 tattccattc atttttcaca gcataaaatc gtttcggtgt aagcggttaa aatagtaagg
    14341 agataaaaat cttgtggtga ccgatttact ctatagtatt caaaagtagt ttctcggtat
    14401 tcgtacaaaa aaaacatact aagattagag gggaacatca ttgacgaatg atgttctcct
    14461 ctattttcta tttttagatt atagtgaaga gtgaaagaaa gttcgaacaa agccttgagg
    14521 actacgagtc cctatcaaaa accgaacttc tttactgtca ttttagaatc aggtttacgt
    14581 atgttggggc catgagcccg tgtataggaa attggaatcg aaatgaagca gtaaagttct
    14641 ttcaacactt aaaaaggagg aaaacaaatg ccttacattc ttgcattaga tgtttcaatg
    14701 ggacacagct acagtgtcct atacaaagac gacacctgct tatttgaaga cgagatcgaa
    14761 cacactcaaa gaggatttca tgcattactc gaagagatcc agcacttgcc tgaacgtcca
    14821 ttgattgttt ttgaagcaac gggtatttat tccagaccag tcgaaaagtt ctttcaagat
    14881 catcactttt cttactgttt gttaaacccg ttagaagcga aaaaacagat ggaagaaaac
    14941 accttacgca gttggaaaac agataaatcg gatgcccatc ggctcgcaca gactcatctt
    15001 aaaaatcaac gtcaaccaaa agaagtacaa agcaattttt acttagagat gcgcgattta
    15061 gcccgttttt atcaagaaat tgagaagaaa atcactcgac tgcgcatgga tttacacaac
    15121 tgccttcagt tgacttttcc agagcttgaa cagttctttt caaacaggct gaccccttac
    15181 gcattgacat tgatccgtct ttttccccat ccagattttg tattggcatc tactcggaca
    15241 aaaataaaaa ataaactgat caacgaaaca cgggaaaaaa tctccgcgaa tcgtgccgag
    15301 caaaaggcag atcaaatcat ccactatgca cagtgtgcct atccagctgt tgaaaaagac
    15361 agtattcact gtcaaaaaac gatctattac gccgaacttc tccaagattt actcgaacaa
    15421 aaaaaagcac tggcgacaca aatgatcaaa aaagccgaag cctctccttg ctttttgttg
    15481 taccaaacgt tccctgggat tggcgcactc acagccgctt tattgctcgg tgaattagga
    15541 gatatcacac gatttaaaac ccataaacaa ttgaatgcct ttgtcggaat cgacatccga
    15601 cggtatcatt caggtaagta tactggccaa gaccgcatca ataagcgtgg taatcccaaa
    15661 gcacggaaaa ttattttctt caccattcga aatatgattc gccagcaacg cgcagctcct
    15721 aatcatattg tcgattatta ctacaaatta aaaaagcaac ctatccctaa gaaagataag
    15781 gttgccacag ttgcttgtat gaacaagctt ctgaaatgta tgcacgctat ggttagggca
    15841 catacagagt acgactatgc gtacgcggtc tcagtggacc attaactaaa ccacttacag
    15901 tataccactt ctttgcccag atcgaaaatt ttttacgaac ggggctttat ttagtgtgcc
    15961 ttgattttgt cgctttcgtt tacgagctat ttttcttgca ccttttactt gactaatcgt
    16021 aggaaagagg ctgtgacaaa attcttgtcg cagctcccta tttcttgatg caggacagct
    16081 ttttcacaac ctctccacta aacggtgttc aatcagctga cc
//
LOCUS       DACIRY010000055        15739 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.056, whole genome shotgun sequence.
ACCESSION   DACIRY010000055 DACIRY010000000
VERSION     DACIRY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.056"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(30..314)
                     /locus_tag="IYE22_001555"
     CDS             complement(30..314)
                     /locus_tag="IYE22_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ9506769.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(304..1038)
                     /locus_tag="IYE22_001556"
     CDS             complement(304..1038)
                     /locus_tag="IYE22_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ9506770.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            1167..1790
                     /locus_tag="IYE22_001557"
     CDS             1167..1790
                     /locus_tag="IYE22_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ9506771.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            1982..3103
                     /locus_tag="IYE22_001558"
     CDS             1982..3103
                     /locus_tag="IYE22_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ9506772.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            3110..6238
                     /locus_tag="IYE22_001559"
     CDS             3110..6238
                     /locus_tag="IYE22_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ9506773.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            6257..9451
                     /locus_tag="IYE22_001560"
     CDS             6257..9451
                     /locus_tag="IYE22_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ9506774.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(9597..10304)
                     /locus_tag="IYE22_001561"
     CDS             complement(9597..10304)
                     /locus_tag="IYE22_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ9506775.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            11069..11467
                     /gene="spxA"
                     /locus_tag="IYE22_001562"
     CDS             11069..11467
                     /gene="spxA"
                     /locus_tag="IYE22_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ9506776.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            11799..12452
                     /locus_tag="IYE22_001563"
     CDS             11799..12452
                     /locus_tag="IYE22_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ9506777.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            12678..13676
                     /locus_tag="IYE22_001564"
     CDS             12678..13676
                     /locus_tag="IYE22_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ9506778.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            13852..15660
                     /gene="pepF"
                     /locus_tag="IYE22_001565"
     CDS             13852..15660
                     /gene="pepF"
                     /locus_tag="IYE22_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ9506779.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         5475 a         2635 c         3243 g         4386 t
ORIGIN      
        1 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
       61 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
      121 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
      181 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
      241 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
      301 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
      361 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
      421 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
      481 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
      541 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
      601 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
      661 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
      721 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
      781 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
      841 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
      901 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
      961 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
     1021 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
     1081 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
     1141 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
     1201 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
     1261 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
     1321 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
     1381 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
     1441 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
     1501 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
     1561 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
     1621 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
     1681 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
     1741 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
     1801 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
     1861 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
     1921 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
     1981 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
     2041 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
     2101 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
     2161 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
     2221 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
     2281 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
     2341 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
     2401 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
     2461 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
     2521 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
     2581 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
     2641 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
     2701 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
     2761 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
     2821 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
     2881 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
     2941 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
     3001 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
     3061 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
     3121 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
     3181 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
     3241 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
     3301 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
     3361 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
     3421 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
     3481 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
     3541 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
     3601 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
     3661 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
     3721 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
     3781 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
     3841 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
     3901 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
     3961 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
     4021 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
     4081 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
     4141 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
     4201 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
     4261 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
     4321 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
     4381 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
     4441 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
     4501 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
     4561 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
     4621 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
     4681 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
     4741 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
     4801 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
     4861 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
     4921 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
     4981 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
     5041 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
     5101 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
     5161 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
     5221 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
     5281 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
     5341 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
     5401 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
     5461 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
     5521 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
     5581 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
     5641 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
     5701 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
     5761 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
     5821 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
     5881 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
     5941 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
     6001 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
     6061 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
     6121 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
     6181 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
     6241 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
     6301 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
     6361 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
     6421 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
     6481 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
     6541 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
     6601 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
     6661 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
     6721 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
     6781 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
     6841 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
     6901 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
     6961 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
     7021 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
     7081 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
     7141 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
     7201 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
     7261 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
     7321 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
     7381 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
     7441 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
     7501 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
     7561 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
     7621 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
     7681 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
     7741 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
     7801 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
     7861 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
     7921 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
     7981 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
     8041 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
     8101 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
     8161 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
     8221 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
     8281 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
     8341 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
     8401 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
     8461 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
     8521 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
     8581 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
     8641 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
     8701 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
     8761 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
     8821 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
     8881 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
     8941 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
     9001 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
     9061 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
     9121 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
     9181 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
     9241 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
     9301 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
     9361 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
     9421 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
     9481 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
     9541 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
     9601 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
     9661 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
     9721 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
     9781 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
     9841 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
     9901 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
     9961 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    10021 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    10081 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    10141 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    10201 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    10261 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    10321 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    10381 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    10441 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    10501 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    10561 agatttattt ttttaataca gtctcaatat aactattcaa acataaaata acgttatttg
    10621 atttatttgt ttatagtaaa ttttatttag taatcattgc tttaaaatac tacgcttata
    10681 taacagagaa aataagcgtt ataataaaga acagtattat ttaaaatgac agaaaacatt
    10741 actattaaag aaaaaaaaaa cataaaaaat cttaagaaat cggtttcatt tagcttttga
    10801 gataaaataa tagtacagtt ttatacgctg tttcatatgt ttagaaaatt tgaattagat
    10861 ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta
    10921 accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt
    10981 tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga
    11041 ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac
    11101 ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa
    11161 tattttttca gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga
    11221 cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt
    11281 agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg
    11341 ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg
    11401 tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg
    11461 attatagaag gataatacga aacttggaaa agactattac aaagatagtt tttccaagtt
    11521 tttttgtttt tacaaaaagt attcttgatt agatgtataa aaaggtagta gaagatgatg
    11581 attgcacgaa aaatagagag ggaaaattag ctgaaaagtc ggctatatag tattcgtctg
    11641 ccaaaaatgt tatagttaaa taagatagct tgaaaggttg aaaaatccgc tggttttatt
    11701 aaattcgctt aacagcgaga ttttttttct ttacatttaa gctgatttcg ctacaatgaa
    11761 tctatattaa acattgtggc aaagtgaggt gtagcaaaat ggaaatggaa catattaatg
    11821 aaaatacgat ccgtgtacta attggtcatg aagatttaga ggaacggggc gtttcatttt
    11881 tggatttact tggcaatcac aaagaaatcg aaaatttctt ttatagtatt ttggaagaag
    11941 ttgatatcga agaggagttc cgaggaagcg aagctgtcac tttccaagtt ttgccaaaag
    12001 gagacggatt agaactgttt atcagtaaaa atcttccgga tgaagaaatt tctcaatcag
    12061 aagattctac tgttgattca gaagatgtga gtgactttct tcgtcagcag attattggca
    12121 gcgatccgga ggaagaatta tctggaagcg ccctagaaga acaggaaaga caagcaactt
    12181 ttgcttttga agattttgaa caagtgatcc aacttgcaac agatgtcgaa ctagagtctg
    12241 cttggactga tttgtattta ttcgaagagc gctattacct agtcgttcac ttctggatgg
    12301 aaaatctcaa ccaagcggat gtagagaatc agattgctcg aattttggag tttgctgaaa
    12361 agagcgatcg tacaccagaa acattgaatg aatatggaca atgtctgatg gcgcgtaatg
    12421 ctatcgagcg aacgaaattt tattttgtat aaatcagcat tatagtaaga gactgtgaca
    12481 aaaattgtgt cgcagtcttt ttttcgtata aatgtgttca aaagcttgca tctgcggtaa
    12541 caagcccaac caaacgaaaa atattgggaa ctatttttgt ttagctgttc ttattacttg
    12601 atgcagaata gctttttcac gccctctttt ttcgtgtaaa taaattctta gacgtaaata
    12661 agttcagagg gagaaaaatg ctgcttgcaa tgaatgaata taataagtgc tgttttgcta
    12721 aacatgcaga aaaaggaaag aaatattatt gtcgtggctg tcgaaaaaga gtgattttga
    12781 aaaagggaaa gaaaaaatgt gctcattttg cccatcaaaa atcagataac tgttcggtct
    12841 ttagtgaaag agaatcaaaa gagcacttac agttaaagga atgcttcatg gattggctgg
    12901 gacagtcagt agaaccagta tttttggaag cctatttgcc gaggttgcgg caaaggccgg
    12961 atattttgtt ggcgaatcta gcaatagaaa tccaatgttc tagactgtct caccagagat
    13021 ttattgaaag aacccaaaat tatttgaata acagttacca agtatggtgg atacttggta
    13081 attcttttct tggacaatcg caattctcat tgatagaaaa aagttgctgc tactataata
    13141 gaaaaagagg agtgcattgc tggaaagctg atctcaagaa acaaaagctt tatttgtatc
    13201 atcatattac agagactgta tcagggcata tttccttttg cagtagctgc tggacttttt
    13261 ctagtaggga ccttaaagaa atattcacta gtaacgaaat taaaatgaac caaatgaaaa
    13321 aaatagaaaa agtgagtgaa gatgggaaga aatggctgac acgccaactt attcacaagc
    13381 aaaagaatac agtatccata caagaacaat gctacttgcg tcataaacat cttttgcatc
    13441 tatcatcgtg gatctatcaa aaaagtcggt ttttctttta tttacaagaa caggtttttc
    13501 tttatcgaat gttgtacgaa gaagccttgg aaaaccagaa agttcctgat ttcaactcat
    13561 ggctttgcca aataaaagaa cataaaaggg aatggctttt tcctatgatt gatgaagaga
    13621 tggtctatca gcagtttttt aacgaatgca tacacttgtc ttctttgaaa aaatagtaag
    13681 tataaaaata ggctaggaat cggttttcta tgtaaaacaa gatttagtta tgctaaaatt
    13741 gatggaagca gacacatgaa tgagtagaaa agttagatgc tgtgacatgt ggatgttaga
    13801 ctatgatcta ttttgataga tagagctaag ggaagaaaag gagtggattt catggaagta
    13861 aagcagttgc caaaacgaga agaattgcct gaaaatttaa cttgggactt gaccaagatc
    13921 ttttcaagcg accaagagtt tgatgagaaa tatttggaat tatcagaaga gttaaaacaa
    13981 tctgaaaaac acaaaggaac acttgatcaa ggcgcttctc aatttttaaa tgcgattgaa
    14041 ttcgtattga gggtttatcg ccaaactgaa gtcatttatg tatatgcgca ccttaaaaac
    14101 gatcaagaca ctggaaatac agattaccaa gcgctttatg caagagcaag cagtctgttt
    14161 tcgaaagtta gtgaagccgt ttcctggttt gaaccagaaa tattgcaatt gtcagatgac
    14221 cagatttggc aatatttcaa agaagaacca aaattggaag tctatcgcca ttatatccag
    14281 caaatagtag ataatcgagc ccatgtctta tctgctgagc aggaatctct tcttgctgga
    14341 gcaggtgaaa tctttgatgc ttcaagtgat acatttgctg ttttgaataa tgcagatcta
    14401 gtttttccaa cgattgaagg agaaaatggt gaaatagtcc aattatctca tggcgtgtat
    14461 ggtcagttgc tagaaagcac ggatcgaagg gtgcgcgaag cagcatttaa ggggttgtac
    14521 agtgtttacg aacaatttag aaatacattt gcttctactt taggcacaca tataaaagga
    14581 cataatttta aagcgaaagt ccgtaattac agctctgcca gagaagcgtc tttgagcaat
    14641 aatcatattc ctgaaagtgt atacgatact ttggtagacg tggtaaacaa gcatttgcct
    14701 ttgttacatc gatacatgga attacggaaa cgtttattag aagtggaaaa actgcacatg
    14761 tatgatcttt atacaccggt cttaggggaa gctccaatta cctttacgta cgaagaagca
    14821 aaagaaaaag ctttagaagc actgaaacca atgggtgaag aatacatggc catcgtagaa
    14881 aaagcattct ctgaacgttg gatcgatgtt gtcgaaaata aagggaaacg aagcggtgct
    14941 tattcttcgg gaagctatga cacaaatcca tatattttat tgaattggca tgatacgctg
    15001 gatcagctat ttacgcttgt ccacgaaatg ggacatagtg ttcatagtta tttcactcgt
    15061 tcgaaccagc cttatgtgta cggcgactac tccatctttt tagcagaaat tgcttcgaca
    15121 acgaatgaaa atatcctaac ggagtattta ttggaaacag aaaaagaccc tcgtgtacgg
    15181 gcttatgtac tcaaccatta cttggatggg tttaaaggaa cagttttccg ccagacacaa
    15241 ttcgctgaat tcgagcattt catgcatacg gaagatgaaa aaggtgtgcc attgactagt
    15301 gaatacctaa gtgatagtta tggtaaattg aatgcaaaat attatggtcc agcagtcgaa
    15361 gaagacccag aaattaaatt cgaatggtca cggataccgc atttttatta taattactat
    15421 gttttccaat actcgactgg cttttctgct gcttcagcac tggcgaagaa aatactaaac
    15481 caggaaccag aggcactaga gaactacttg gcttacctaa aagcaggtaa cagcgattat
    15541 cctgtggaag taatgaaaaa agcaggagtt gatatgacac aggctgcata tattgaagat
    15601 gcaatgtcga tgtttgaaca acgtctaaat gaattagaag aattgatcga tcgtttataa
    15661 ggctttctat ttttgatttt ctcgaataga aacgaaaaaa atacgtcggc tctcttgtaa
    15721 gggagtcgac gtatttttt
//
LOCUS       DACIRY010000056        15724 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.057, whole genome shotgun sequence.
ACCESSION   DACIRY010000056 DACIRY010000000
VERSION     DACIRY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.057"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..2179)
                     /gene="nrdD"
                     /locus_tag="IYE22_001566"
     CDS             complement(<1..2179)
                     /gene="nrdD"
                     /locus_tag="IYE22_001566"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ9506780.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVK"
     gene            2505..3272
                     /locus_tag="IYE22_001567"
     CDS             2505..3272
                     /locus_tag="IYE22_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506781.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            3262..5304
                     /locus_tag="IYE22_001568"
     CDS             3262..5304
                     /locus_tag="IYE22_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506782.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            5585..5734
                     /locus_tag="IYE22_001569"
     CDS             5585..5734
                     /locus_tag="IYE22_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ9506783.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            5745..7259
                     /gene="dltA"
                     /locus_tag="IYE22_001570"
     CDS             5745..7259
                     /gene="dltA"
                     /locus_tag="IYE22_001570"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ9506784.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            7256..8461
                     /gene="dltB"
                     /locus_tag="IYE22_001571"
     CDS             7256..8461
                     /gene="dltB"
                     /locus_tag="IYE22_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ9506785.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            8506..8739
                     /gene="dltC"
                     /locus_tag="IYE22_001572"
     CDS             8506..8739
                     /gene="dltC"
                     /locus_tag="IYE22_001572"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ9506786.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            8742..10010
                     /gene="dltD"
                     /locus_tag="IYE22_001573"
     CDS             8742..10010
                     /gene="dltD"
                     /locus_tag="IYE22_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ9506787.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            10151..11233
                     /locus_tag="IYE22_001574"
     CDS             10151..11233
                     /locus_tag="IYE22_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ9506788.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            11233..12036
                     /locus_tag="IYE22_001575"
     CDS             11233..12036
                     /locus_tag="IYE22_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ9506789.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            12106..12846
                     /locus_tag="IYE22_001576"
     CDS             12106..12846
                     /locus_tag="IYE22_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ9506790.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            12857..13861
                     /locus_tag="IYE22_001577"
     CDS             12857..13861
                     /locus_tag="IYE22_001577"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ9506791.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(13906..15669)
                     /locus_tag="IYE22_001578"
     CDS             complement(13906..15669)
                     /locus_tag="IYE22_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506792.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         4922 a         2649 c         3132 g         5021 t
ORIGIN      
        1 gtttcacacg tgaggaaatt tctttatggc ggccatgtac catcggacga gcttgcggat
       61 ttcctaaata gccacaagta cgtttgacta catcacatgt ttttggatca tgatttccac
      121 attctggaca ttcgaaccct cgttctgtcg gtttgaaatc accttcgaaa ccacattgat
      181 agcaatgatc gattggcgta ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc
      241 atacagcttc taaagctttc ggattttgtt ggagcatagg gtattcacag tagtggatga
      301 atccacctga acaatatttt ggataatcct tctcaaattc caatttttca aatggcgttg
      361 ggtttttacg cacatcatag tggaaactgt ttgtataata ttctttatct gtgatatttt
      421 cgatgatgcc gaatttttct gtatccaaac ggcagaaacg gtcagttaga ctttcacttg
      481 gagttgaata gacactgaaa tgataaccat attcatcccc ccatgcatct gcattagctt
      541 ttaattcttt gacgatatca agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc
      601 caccgaagaa agctgaagca acttcataca atccaatgta gcctaacgat acagtagctc
      661 gacggttttt gaataattca ttgacagaat cggtacgtga aagacgtttc ccgaaagcac
      721 catacatata taaaattggc gcatttgctg gaatggcttc tttacaacgt tctacacgat
      781 agagaagcgc atctttcatg atttctaagc ggtcagacag taactgccag aatttattct
      841 gatctccttt tgattcgatc gcgattcttg gcaaattcaa tgtaacaaca cctaaattca
      901 ttcgtccgac atttacttct tgaccatttt cgtctttcca cccttgcaag aatgaacgac
      961 agcccatagg tactttgaaa cttccagtta attcaacgat tttatcgtaa ttcagaatat
     1021 caggatacat tcttttcgtt gcacattcca atgctagttg tttgatgtcg taattaggat
     1081 cttcagggtt taagttcaca ccttttttca aagtgaagat cagtttaggg aagatagctg
     1141 tccgcttttc actccctaat ccattgattc gaatctgtaa aattgctttt tgaatttctc
     1201 tttcaaacca gttttctcct agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg
     1261 aagtaaataa ggtattgatt tcatattcca aactttgcat agcatcgaag atatcttttt
     1321 tggtttttgc ttttgcatat tctttttggc gttctggcga atcgatccat tcttccgcgt
     1381 cttttagatg ttttttatag ttcaattctg caaaaggagc aagcaattca tctgttcggt
     1441 cggcagaaca gccaccatat tgacttgacg ctacattggc aataatctgt gagatttgtg
     1501 ctgtggcggt ctggatagat tttggcgatt cgacttcggc attgccgatt ttgaacccat
     1561 tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt catcggtgta taaggatgat
     1621 aatccaaatc atggtaatgg atatcgcctt tttgatgcgc atttgctata tgtggtggca
     1681 acattttcag tccgattgat tttccaacga tcccggctgt cagatctcgc tgtgtattga
     1741 aaacattact gtctttattt gcgttttcgt tcacgacagt ttggtccttg ttgattagct
     1801 tttcaatcga aaagttgata tctgttgctt tactgcgagc aaaatctcgt ttggtacgat
     1861 aattgatgta ctcttctgct aattcatatt cgtttttttc tagtaatata tgttccacga
     1921 tgttttgaat ctcatagatt ttgacatttt cagcaaaccg gcttttgatt tcttggacaa
     1981 ccaagtctgc aatcatttga attttttcat aagtaagtgg tgataatgag ccgtgaattt
     2041 tcttttctgc tttgatcagt gcttcattga tcttttgttc atcgaaatca acatggcggc
     2101 catcgcgttt gatgactttc atctgggatg tctctggctg ctgagtaacg acagtttttt
     2161 cttcataatt tactttcatt atcttcaact cctgctaaat tgttcttgtc tatcataaaa
     2221 caaaatcggg gaaggagcaa ctaaatatcg gctatatata gtattcaatg tgctgtataa
     2281 ttggatttct atactatatg tagtgtatat tttattgtga aaaaaggtga aaacttgtag
     2341 ctggtggatt tgttttttgc atatttaatt ttgctgaata aaataattga ataaaatttt
     2401 ttattcagtc caagtaaaag tgacaaaaac ataagagcaa attattcgta gatatgctac
     2461 tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga agaattggaa aagatgatcg
     2521 aagtgaaaca tctaagtaaa agctacggca gcaaactttc tcccgtcaaa gccttagata
     2581 atatctcatt cacagtagaa gaaggcgagt ttgttgggat tatgggccca agtggtgcag
     2641 gaaagacaac gctgatgaat atcttgtcca cgatcaattt gcctacgatg ggcagtgtct
     2701 cgatccaagg aaaagaaatc acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa
     2761 aaaaactagg attcattttt caagagttca atttgatcga taccctaaat gcaaaagaca
     2821 atatcctatt gcccctagca gttgaacgga tgactaaaga ggaaatggaa aagagagtaa
     2881 gacatgtagc tcaattgttg aatatagaag aactgctcaa acggtatccc gatgaacttt
     2941 ctgtcggaca gagacaacga attgcagctg ctagagcact tgttgttcag ccacaagtga
     3001 tttttgcaga tgaaccaact ggctctttgg actcgaagtc ggctacagaa ttgttgaact
     3061 acttgaagac catgaatcaa aaagaaaaag caacgatact gttagtcacc catgatccat
     3121 atacagcaag ctactgcgat agaattttat ttattaaaga tggtgtgatt ttttctgaag
     3181 ttgtcagaag agggacaaga agagagttct ttgagaaagt aatagacatg caggcgacga
     3241 ttggcggagg aggaaaaatg aatgctgtat aaactagccg ttaaaagttt ttttcggcag
     3301 tcacgaggct atcttgttta ttttttcagt ttgactttat ccgtaatgat ctattattcc
     3361 ttcagcgcaa tgacctacga tcaatcttta gtcagaagag caagtcaaga tacttcgata
     3421 gatggtggat tgagttttgg tggatttatc attactgttg ttttgctttt tttcgtattc
     3481 agtgccaacc gatttttctt gaatagaagg caaaaggaaa taggcatcta tcagcttttt
     3541 ggtatgaata aactgcagat atcaggtata tatgtgttag aaatcatgat aattggattg
     3601 ttcgcctgta tttctgggat attactcggt attatttttt ccaaactctt ttcaatgatt
     3661 ttggttcgaa tgatggatat ggatcttact agtccatttt ttatctccat tccatcagta
     3721 gtagatactt tatttgcttt tttcatcatc cttttagcag tatctgtata ttcaatatgg
     3781 aaaatatggc gctatccaat gattcgaagt tttggagaaa aagaacaagc agatagttca
     3841 acaatgcgct tcaggacaag gcaccgatta ctcgcagtca tcgggttact acttttaacg
     3901 agcggatatt ttggagctag tcattttagg gaaatcactt catggatgat cagttatcat
     3961 tatcttgata cattagtttt cttttttcct tttttaattt ttttcgtttg tgtggtcggt
     4021 acttatttgt ttttctgtta tacatttcgg ttatttttga tcatattgag taaatggaag
     4081 atttattact atggtatcaa ttttttgata atgggtaaca cgcaagttca tttgctcaaa
     4141 agttggcgta caaattctct gattacagtc attctcggaa tttctttaat gacaattggc
     4201 ggcacggcta gtatctctac tattttatcc taccaggaga aagtttcctc cccgatggac
     4261 tatcagtttg atgttgagac agagaaaaaa atcaagccga ttctagcaga ggaagggcag
     4321 aagataacta aagaaatcac cttgcattat aaagtcattg ggagtatcta tagtcaaaat
     4381 ctcgaagaac ctatgtttca aactgctaat ctgatcacgg aaacacaata tcaagccttc
     4441 agaaaagtta atccagattt gccgaacatt tctttgaaat cacctggtca tactgttttg
     4501 cttgatcaaa tgcaaactct ttttcggaat atttcattat atggatctag tattggactg
     4561 ccagataatc aagaattgaa gattcaacaa atgcttccaa gtgtattagg tgacgaaaca
     4621 atggtatatc taagtcctgt actgattgta gatgataccg tttttgaaaa agcaaaaggt
     4681 gtggattatc aagtagtcaa tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa
     4741 cgactgagca atgacctgtc gataaagtgg ctcaatgctg tttattattc ttatgatatc
     4801 gtaaatggaa gtctacaagg agaaattagc acaaagaaac ttccggatga ccaaatgaag
     4861 gaagcacaat ttagccaaga aagctctcgt ttgaatttct cttccagcta ttcgaaattg
     4921 cgtgcagtta atcggcgaat cggtatcaat atttttgtca cactatttgt cgggatgatc
     4981 gtgattattg ctactggtag tattttgaca gtccggcaat tagcagaagc agaggaagaa
     5041 aaagaaaatt atcacctatt gactaagctg gggattccac aaagaaggat cagacggatg
     5101 atcttcgagc aaaacgcact taccttcttt ccacccatga tattagggat cactcacgca
     5161 gtatttgcaa tcaatgtctt cactcagtat ataaaaactg ccgattactg gttggcttat
     5221 cttgtgagtg gcttattgat ccttatctat cttttgcttt atgtcattac gagtcagtta
     5281 tattgtcgga ttatcgagga atgatatacg gaataagaca agataataaa tagaactaaa
     5341 ttttgaatac catttatttg gaataagagg ttgtgacacg atttttgtca caacctcttt
     5401 attttttata ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg
     5461 ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc tgtaagctag
     5521 acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat gaggagggaa
     5581 tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat tcatcattcg
     5641 aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact ataaaaatat
     5701 tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa atcaaaacga
     5761 ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct tatcaggcgg
     5821 atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct tattatttag
     5881 aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag tttgaaatga
     5941 ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt gaagagcata
     6001 cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg gtcatcagta
     6061 tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag
     6121 aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt acagaaaatt
     6181 tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa atcagtcatg
     6241 ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag cctggtatgc
     6301 atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt tatcctgcat
     6361 tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac tttcgtcaat
     6421 tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca tcatttatgg
     6481 atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg gacatgtttt
     6541 tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa
     6601 atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt tcaagtattc
     6661 agattgacca gcatgtacta gatagttatg accgactacc gattggctac gtaaaaaaag
     6721 acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa acgggtgaaa
     6781 tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa aagacagctg
     6841 ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca ggtagactag
     6901 atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa ttacatggat
     6961 accggattga actcggggat atcgaacatt atctgctcca agacaaccga atcaaacaag
     7021 ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct tttgtcgtat
     7081 tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa gaacaattac
     7141 tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat ctgctgcctc
     7201 agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat
     7261 gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag ccattgcgct
     7321 tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt atcaatcagc
     7381 agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac agggagcggc
     7441 gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg tttatcggca
     7501 aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa ttccactgtt
     7561 tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg gttttttagg
     7621 aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag atggcatgat
     7681 caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa caatctcttc
     7741 tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc ctgttcgtga
     7801 taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa
     7861 attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac gcattgcctt
     7921 acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca gtatgtattt
     7981 gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca tgatgggata
     8041 tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag aattctggaa
     8101 tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc gattgatgtt
     8161 cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata tcggttattt
     8221 tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca tcgtatatgg
     8281 actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta agaagaagca
     8341 taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt tgacatttca
     8401 agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt tttttagcta
     8461 acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga agaacaaatt
     8521 ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat
     8581 ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt cgagctagac
     8641 ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg gagcacaccg
     8701 aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag aaactttttg
     8761 ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt acctcctcct
     8821 atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga
     8881 acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa tatattcctt
     8941 tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt ctggcagaaa
     9001 aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa tcgttaagcc
     9061 aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc gtctttatta
     9121 tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat acgtattatt
     9181 ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct gctgatcgct
     9241 atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg ttgacggcga
     9301 ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat cagctgcgca
     9361 gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg ttgaaagatc
     9421 gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa gaaacagaat
     9481 tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca ttcgggttga
     9541 aaaacgattt ttatacgcat cgtatccgag cccacgagcc agagctcaaa cagtcacaaa
     9601 aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc ttggatcaat
     9661 tagcaaaaaa ccataacgaa gtattgttca tcatcccacc ggttaacgag aaatggagcg
     9721 attataccgg actatctcaa gaaatgttac aaggatttgc caaaaagatc aaatttcaat
     9781 taaatagcca aggattcaat cgaatagctg attttgtcaa tcaagcgggt accaactatt
     9841 ttatggaaga cacgatccat ctaggctgga aaggctggtt ggctgcagat caacagatca
     9901 gaccgttttt agaagaaaat catattactg ctagtaagta tcatcttgac gatgcttttt
     9961 ttagcaaatc ttggcaacat caaataccag ataaactaca attaaaataa agaaaacagg
    10021 cgagtgttgc tcgcttgttt tctttatttt tgaatattct gaataaaaga taaaaatacc
    10081 aagtttgcct gaacaaaaat cataagcaat ggtataatca caatatgaat atagaaatgg
    10141 gggaaaacat ttggatacaa aagaagaaca gctacttatt gatttaactt ctgcaaaagg
    10201 agtacctgga aatgaagaag aggtaagaga agtattcaga gaatatgcca aaccattcgc
    10261 tgatgatatt ttttatgacg gtttaggcag tgtgattgcc aaacatggtg ctggcggagg
    10321 ccccaaggtc tttatttctg gacatatgga cgaagttggt tttatggtaa caaaaataac
    10381 cgaaaaagga ttcttggaat tccaaactct tggaggttgg tgggggcaag tcatgcttgc
    10441 tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa
    10501 accaccacat gttttaacac ctcaagtaag aaataaacct tatgaaataa aggatatgtt
    10561 cattgatatt ggcgcatcaa gccaagaaga agccaaagag tggggcatac gtcctggaga
    10621 tatggtcact ccatatattg aatacaaacg aatgaatggc agtaaatatt tactggcaaa
    10681 agcatgggat aatcgtatcg ggacagctgt ttctctgcga gtattagaga atctatctaa
    10741 agaagcacac ccaaatgttt tattcgcagg aagcgatgtg caagaagaag taggcttgcg
    10801 aggtgctcga acaagtacac accttgtaaa tcccgatatt gcttttgctt tagatactgg
    10861 tacagcagga gacactccag gaatgacacc aaaagaagca gattcgattt taggaaaagg
    10921 cccacagatt ttgatttttg atgcatctat gatcccgcat aaaaaattat tgaattttgt
    10981 tattgatgta gcagaggaaa tggatattcc attccaatat acagtgattt ctggcggagg
    11041 aactgatgca ggacaaatgc atttgacaag agatggtgtt ccgtctgtag ccattaccgt
    11101 tcctgttcgt tatctgcatt cccatacatc agtgattcat gaagatgatt atttgaatac
    11161 agtgaaacta gtcacagaat tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg
    11221 gagttatgaa taatgaataa tagaatacct atttctgaat atcggaaaaa agcacgagtt
    11281 tctttggaaa atcaatgggg aatcaatact tggcttgttt tcttatctat aatatttacg
    11341 agcgtcatcc agaacttttt tgacggtttc agtccttttc cggaagaaag catgcagcag
    11401 cacttattga gttttctagt cgataattta cttttattcg cattcaacta tgcattgttt
    11461 tacattgcgc tagtagttgt acggggtggc agaccaaaaa gtaacctttt attttcagtt
    11521 ttccaaagaa agtattttgg ttcgatactt ggcatcaatt tgatcaattc agtcatcaac
    11581 tggctactca acagtttaat ctttctgccc atgtttttaa tcagcggagt gagtacttat
    11641 atgcagctgc ttttcagttc aaatgcggta cctacgggtg tcatgccaga agattttctt
    11701 gatatcagct ttgttttatc gatgataggg atgatttttg tttcagcact catcatgagt
    11761 attgttggcg gtttattcca atttgctgca tggacaaagt tcgactatcc tgatttgtcg
    11821 gttattcaat gtttgaaata tggctggttt ttattgaaag atagaattgg tacgtatatt
    11881 ttgttgcagc tgtcttttat cggctggtat ttgctaggag caatcgcttt gttcatcggt
    11941 cttttgtggg tcattgttta tgtgaatgtg tcagttgcag aattttatga acaagcacgg
    12001 attgaaaaag gaggtccaga cgcctatttt acttagcatg taagcagttt attcatcaaa
    12061 aaaataactt ttgtggtagg ataaatcttt gagggggaat gtacgatgaa attaactgtt
    12121 ttaggatgct taggagcgta tccttataaa aatcaaggga caacaggtta tttgcttcaa
    12181 tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa attacaagaa
    12241 caccttgatc cacttgatct agatgctgtg atccttagcc attatcatca tgatcatatt
    12301 gctgaccttg gtgtattaca gtattactgg caacttcatc ctaagagaaa tccggagaca
    12361 ttgctttcaa tttatggaca cacaaaagat tccattcact ttgaagaatt gacgttggaa
    12421 aatgtctcaa aaggacatcc ttattttgaa gctgaagaat taaaaattgg tccttttctg
    12481 attactttta tggaaacaat ccatccagta gtatgttacg ccatgcgtat cgtagaagaa
    12541 acaacgggga aagtattcgt atttacaggt gattcaggtt atttggaaag tttcgctgat
    12601 tttgcgaaag atgctgattt gtttctagca gatacctatc tttttgaagg aaatgaacat
    12661 catcatgccc atttcacttc aaaagaagct ggagaacttg cgaaatcagc taacgtaaag
    12721 aaacttgtac ttacacacct acctcagttt ggtgatttag aacaattgaa aaatgaagcg
    12781 caaaaggcag caggcaaagg gattgatata gaacttgctg cagttgacaa agtgtttaac
    12841 atttaggagg aagaacatga tttttgtacc aaatgaaaac cacgacccaa gaatcaattt
    12901 agcaattgaa caatatttgc tgcaagaaat gcctttagat gaacctattt tgctctttta
    12961 tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg
    13021 agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt
    13081 ctaccatgat tttggaaacc tgaactttag ttttatcatg cctgatgatg gggattcatt
    13141 ccgtgacttt gcgaaagtaa cacagccaat catccaagca cttcatgaat tgggtgttgc
    13201 tggggcggaa ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat tttctggcaa
    13261 cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga acaattatgt tcgactctga
    13321 tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat
    13381 caaatcgatc cgttctcgtg taacgaatat caaaccattt cttccagaag aaaagcaaaa
    13441 aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa attttcggcg tagagtcagt
    13501 agaagaagta aaaacgtacg aattgactga tgaagattgg aaaaaaatca atcgaatctc
    13561 tgaggaatat tatcgaaatt gggattggaa ttacgggaag tctccagatt tcaatttctc
    13621 tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat ttgaatgtct cagaaggaat
    13681 gatcaaagaa gcaaaaatct ttggcgattt cttcggacta ggagatatct ctgacgtgga
    13741 aaaaaaattg gttggacaga aatatgataa agcatctcta tcaaaaatag tcagccaaat
    13801 tgatgtgaag aaatattttg gtgcgataga accagaagaa ttgcttacct tactttattg
    13861 aaaataaaaa atagcggaat ccacatatat ggattccgct attttttata caggattcgg
    13921 gtaatttcga gagagttgaa cagtgatccg atcaatgaaa gtatcttttt cctgcgttag
    13981 tgttcctgtg taacgatccg ctacgtcttt catcatcatt aagtcaaaac gttcctgatc
    14041 atctaattct tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg cactatgttc
    14101 gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac aaataaattt ttcgatacag
    14161 tggattttcg atggcatgag tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg
    14221 agccagatcg tcatcgttga taggaagttc ttcgggaaga tctgcttgga aggttatctg
    14281 aatattccgt ttggcagcta acttctggta acgcgctaag ataagatttg tcacctggtg
    14341 gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct tctaatatct tttccacata
    14401 gtttttagct tttgtttggt ccgtatgcat taaatcttgc agattctcca tctcttggac
    14461 aaattccagg cgaagtgtat ttacttcttg gatatgctgt cgaagctgct cataatgttg
    14521 ctccaacaaa tcatttttga tttttaagaa atctacttgt ctccgatctt tgtcggtatt
    14581 taagatgtga tcgattagat tgtagctgat aataacgatt agaatgataa tatataaaat
    14641 cgtagaccaa ttgatcgttg tttgatagat acctaatgcc gtttggatgt aaatcaagca
    14701 atggataatg gctaccaaga cgatccaagg tgtacaagct acataaaaac ggttattctt
    14761 atatgcttcg ccaatgcatg caagtgaagt gactaaagtg cttataacac tagcaatatt
    14821 agcaatcggc attaattcag gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc
    14881 gataaaaatc aaaagttgga acgtacaaaa aattccgtac cattttttat agtaaaacat
    14941 tcggctccaa tagtagcaga tgatgaaagc tgacgttaag ataataaata aatgagataa
    15001 aaaagaatga acgaatggtt caaaaagaat accacttaaa atatcttctg atacagcttc
    15061 aaatcctaat gccaaagcaa agcaacttaa gagaataagt gagtattcaa gacgtttttg
    15121 tttatacatc aagtagatcg tatagaaaaa ggtccctata gcgataaaaa cagcgattat
    15181 catcgtcaat acttgcggca ctgattgtga ataaataaaa ctaatcaatg gtccagttgt
    15241 tcctagataa actttcggtg gcagtccagc atagttctga taaggagatg agactgtaag
    15301 ggtcaatttt tttccgatat aatttgctgg taaatctatc acagccaatg agagtccggg
    15361 attgctttct tcctgataat ctttccgttg gtataaaatc ttttcgccta gatatacagt
    15421 aatccattga tggtttcctt tgattagtaa atttgcttcg tccaacggtt tttcaagtgt
    15481 cgtttccata atgaatgttt cgtctttatc cacggtagga atcgtacgag tgtacttgct
    15541 gtcaaactta aaatcaggat catcttttgt atagaaaacc cacgaatctt ttatttcttt
    15601 catttgattg ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa ttcctgttaa
    15661 aaataacaat gataatataa tgattatgct ccactttata cgtaaataaa aagattcttt
    15721 ttta
//
LOCUS       DACIRY010000057        15695 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.058, whole genome shotgun sequence.
ACCESSION   DACIRY010000057 DACIRY010000000
VERSION     DACIRY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.058"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1110)
                     /locus_tag="IYE22_001579"
     CDS             complement(<1..1110)
                     /locus_tag="IYE22_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ9506793.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKK"
     gene            1171..1911
                     /locus_tag="IYE22_001580"
     CDS             1171..1911
                     /locus_tag="IYE22_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9506794.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1997..2755)
                     /locus_tag="IYE22_001581"
     CDS             complement(1997..2755)
                     /locus_tag="IYE22_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ9506795.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2752..3780)
                     /locus_tag="IYE22_001582"
                     /pseudo
     CDS             complement(2752..3780)
                     /locus_tag="IYE22_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(3827..6967)
                     /locus_tag="IYE22_001583"
     CDS             complement(3827..6967)
                     /locus_tag="IYE22_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506796.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            complement(7191..8075)
                     /locus_tag="IYE22_001584"
     CDS             complement(7191..8075)
                     /locus_tag="IYE22_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ9506797.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(8170..8607)
                     /locus_tag="IYE22_001585"
     CDS             complement(8170..8607)
                     /locus_tag="IYE22_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506798.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(8824..9735)
                     /locus_tag="IYE22_001586"
     CDS             complement(8824..9735)
                     /locus_tag="IYE22_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9506799.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(10192..10620)
                     /locus_tag="IYE22_001587"
     CDS             complement(10192..10620)
                     /locus_tag="IYE22_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506800.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(10648..11286)
                     /locus_tag="IYE22_001588"
     CDS             complement(10648..11286)
                     /locus_tag="IYE22_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506801.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(11290..13326)
                     /locus_tag="IYE22_001589"
     CDS             complement(11290..13326)
                     /locus_tag="IYE22_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ9506802.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            13452..14117
                     /locus_tag="IYE22_001590"
     CDS             13452..14117
                     /locus_tag="IYE22_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9506803.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT         4759 a         3100 c         2553 g         5283 t
ORIGIN      
        1 ttttttagag gtttttcctc ttttgaccat ctccgatagc gtcaaattcg ttggatttgc
       61 cttgttcgct atactaaaac gtgatccctc tttttcttcc agcagattga tcgtaatcgg
      121 actctgatcg gctacacgat gatcgaatgc gttagaaata aaaatagata aacagcgaat
      181 aaaaacaacc agatcaatcg ggattgtttt cacttcgaca ggaataacta cttggatcgg
      241 gattttctcc gattctgctt tttctagaaa ttttaaaaag acacccctga cagctgggga
      301 tacaatattt gttagctgtt gtatttgcgg tttttcaaaa atagctgcag aatgatcttc
      361 taatatttct aaaaattcca acgtttcttt tttattttcc tgaagagtaa tttttaatga
      421 aaacagtaga cttttataat catgtttgaa ttcctctaga tgatggtaat attcttcatt
      481 tatttgatac agttgaagcg attcctgatg ttgtctttct tttacttgga aactgatcaa
      541 cataatggaa aaaaacaaag aaaaaaaact gatgactatc gacaaaagag atagaagcag
      601 ataattagat gagcggaagt gaacacttcc caataccaag taaatcacgc cccagcttaa
      661 taaaatacaa atacctccaa aatagtgttc agttggataa gtaaaaagca agttgagaaa
      721 tctcgtctgt ttgtctatac tttttactaa tagatagaaa ataaatgcga taagtagtgc
      781 aaaaaaactt gctgttccaa gatgtctctt tcctaaataa tccatcagat caaaaaccag
      841 aaaaaagctg agtccagtga tagcaaaaaa aagattactg atcaaacagc tgataaacag
      901 gtttttcgta gtaaaataaa aaagaaacat caataataag ccaattaggt aaggaaagct
      961 cactttaggg ataggaagta aaagtgtcac caccatcaaa gctatacaac caaagctgtt
     1021 ttttactagc agttgtttcg aaaagaataa atagatacct aaatctaaaa tcaaagtaaa
     1081 ggtcaaataa agtgtcactg ttgcgaccat cttattcctc cattgttttg tttcaaaatg
     1141 tacacatatt tttttaatag gagcctgcat atgaaaaagt attttatcat agaagataac
     1201 cagtttcaca gcgaatggat catcagagaa ttggtcaatc atcatatcga gaatatcgtg
     1261 cagctcactt ctatctctca aatcaatcag ttgatccagt tgggcactat tgataatcac
     1321 gatgttttct ttatcgatat cgacttgaag caatcactca caggtattga tgtcgccgaa
     1381 caaatacgta aaaaaaatgc acatagtaat attgtttttt tcaccgctta tacgaatttt
     1441 gcagtagaga tcatcaatcg gaaaatcctt ccgattggat atatcagaaa agacaggaat
     1501 ttatctgaac aaattcgtca agtgctagaa cagatcaatt ggaaagaaca gcagatcgta
     1561 aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa
     1621 attttatata ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa aacaactaca
     1681 gaagataaat tagtgaatac ttctttaaaa caattgaaga cagagttaga aaagtatgct
     1741 tactttttac ttttaaaatc ttactgtatc aacttatcgc aattgcagaa aattgattct
     1801 gctcattatg tgttagaatt tttcaacggt gactccttac tcgttggtcg aaagatcttt
     1861 gaaaagacaa aggaacgttt ccatgacttc caaaaaactg cttcgagttg atgaataaga
     1921 gctgagacgt aagcatagca gatttcaggg agtgggcctt gaatcggctc cactccctga
     1981 agtcgttgat atgattttac gcttcctctt ctgtaggagc atctgttaac gaccatgtga
     2041 atgttgaaac gtagttgtct acagcataat tggatacagg aacaaataat tccactgatt
     2101 gtctcccttg ttccacattc tctcctaatg aataaatcca tgtgccaatc ccttcatttg
     2161 ctcctgcagt tactagatct actgcctgat ctgctgggat ttcgattcct tcagcgccag
     2221 ttccagaaac ggtagatggc attccactgc tattagttgc gctatttcga atcacggcgt
     2281 ttttgaattt cacctttgaa ccagttagtt tattcgtatc agatgttgta ccaataaatt
     2341 ctgttcgttt gactgataat ttccaaccgg cataatttcc gcttttatcc gttacttgta
     2401 cgaaattctg tacttgtgtt tgtgctccag tactgtccgt tccgctttgt aatgcagcat
     2461 atactgtttt tgctgtacct aacttgattg ttccaaaatc aagatctgga gcttggtcaa
     2521 tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga gtatcaggat
     2581 catttggatc aacaggagga actggaacaa cgtcttcagg atttgcagca tctggtttgg
     2641 tcacagaaat cgttccttct gtagtggctg agctcttatt ctcagcaaaa acactttgtg
     2701 tacataataa tagacttcca gctagaacgg tacttaatgc aattgttttt ttcatttctt
     2761 tttccttctt tcttttaaaa taaataatcc agcaaataag ccaataagaa tgactccaac
     2821 aagtatcagc catcgccagt caaatgtttc tggctgattt tttaaatcaa tatccttttc
     2881 attcaattgg tccgctgcat catcagttat ttcaaaatcg gtatggagat tccagcgttt
     2941 ttctttccaa gcacccttca caaccgcttg ataatgtccc ggagctaatt tttttccttc
     3001 taatgggaca agtaaatcaa aatttgtatt gggtgctaca tctatttttt ctttttttat
     3061 tgaaaggtaa ggctcttttg tttctttttt gtagatttca accgatgtag tcaagccgaa
     3121 catataagaa gcatttggat tttgaagatt cgccttaatc acatttcttc catcgctttg
     3181 atccgcataa gcttttttta atttcaaatc tggggaagac tctttttcgc tatttcaaat
     3241 caagattgct ttcacttgag aaaactgatt gacgacacca actgtacttt tgttagaaac
     3301 gttctctttt ttttcttgaa acgtgatccc tcctgcgacc acaccagcca gctctttttc
     3361 tggcgctttc agtgaaagtg tctcagtgac cgcactttga ggaggaacag ttatttctcc
     3421 cactgttttt gctaagtttt tcatatcata acttaatgtt tcatcaaatt tactttttga
     3481 ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca atgtctcaac
     3541 tgttactgat ttatctgttc ggttaaagac cgtgtagggg atttcctgtg ttttcccagg
     3601 ctccatcctt acatttatat atgttttgtt cttatcaatc tgattagccg gtaattcaaa
     3661 atctaccgca aaattgaatt cggaggcatg gacaggtgtc tgccaaagga tgaatccaag
     3721 gagaaggatc ataagtaaag agcagttgtt tcgatgactt ttattccata ttcgattcat
     3781 caatcatcct ccttgatctt tattctgtcg gcgcatctgc taatgtctat gttatctctg
     3841 ctgagtaatt accagctatc tgattttctt tggttaagac aagtttaaac gcccgaccgt
     3901 tatttaaata ttctgaataa tctgcttgca cattcgttga attttgtcct ctgacaattt
     3961 ccagttcttc ttcattcact aattgatact cctctccttt cttgaaatat aggacatcct
     4021 gcatagaaaa tcctgcatca tttgtgaaag gctgagataa ttttatcgtt aagatccacg
     4081 gatttttcgt ttctcgctga tcataaatcc ataatttttc ctgtatatcc atttggccaa
     4141 atgcttcttg tgtcaaacca gttgctttga tctgaccaaa atcgagtttg gaaggggcaa
     4201 gaaatgagag accaccttct actacgatct tcgtagcagg tgcaaaaggc ggggcatcat
     4261 gataaacaac tggcgtatca cttggattgt gattcccaat ttcatcgttg gtacttggct
     4321 tgttctgcac acccttagca gtagcttctc ctgcttggtc gtttaataca atttctaata
     4381 catctcctgc aaaaagttga ttttctggga tagaaagaac aaattcccct gtatcatcaa
     4441 tagttgtttc tgacaaaaca gcttgatttt ttttcaatga aaccaaggct cctgcttcac
     4501 ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa ttagcaggtt
     4561 caggaggaat cacatcgact gtcgttttgg cagagtccag ttctgtcact tcttggccac
     4621 ctcttacttt tttagcagca acaacttgca cttgatcctc cgtttttaat ctctcatcat
     4681 tgggtacttg aacttcgaac aagccgcctt gttcgccttc tccccatggg ttatatcctg
     4741 ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt aatttctaca gatgatttcg
     4801 tcgtatctgc gtaagtaata cgtaaagtca cttctacttc gcctgtccaa gcatctcttg
     4861 gctttgcctc gacaccttcg ggaacaacga catgcccata gattttttta tctgcattcg
     4921 ttggcactct taaggattca atcatcggga gctgattatt ggcactgatt cgattataat
     4981 ttttcattcc ggcacctgtc tgagcaaatt catttgttaa acctgcacta gggcttgata
     5041 cgaaattgcc taagtcggtc ccattgatcg ataagtctgt catatcagaa aaataatccg
     5101 ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg atttctgaat
     5161 gggcgatctt taaagaagaa tctccatttc cttggaatac ccagccgcct atgccattgg
     5221 tctcgcttcc aatccgtttg ttttggaaat caaaataggt aggagattct aagtccacat
     5281 tcaacacatc cccactgatg atgccggtct tatcttcccc tgttttcccg accatgacta
     5341 aataagtttt ttcacctgcc gtaaaattta aatcaatatc tccagttgta tctacagttg
     5401 ctccggaatc tgcgcgtata tcgatatttg atttttctcc agtgactgta aaatcagctg
     5461 aacctggact ttctagaaaa ccagaagcgt cataaaattg gatcgcttga tttccttgat
     5521 tgagtctgtt cacgtaagat ttgccatcac ctgtatttct taccctcaat gagcctccat
     5581 tggttactgt cagcttttgc gtaccattct caaaacgaac tgcgctggaa cgcccactct
     5641 gcttatcgat gtcgataact gatccggctc cttctacgat caaagaaagg ccttttccta
     5701 caaaacgcat cccattgttc ccgggttgat cgtcatctcc agccattttg accgagattt
     5761 gtccaccact tgatacatgg atttgactag aatctccttg caagcgaaat ccgcccgtag
     5821 ttgctgtatt gatggtgatt ttcccaccat tcgatacagt gactttcgat cctgaacctc
     5881 tcatttggat gattccgcga ttgttgtcat tggtaatatt gatctttgta tttaccgaaa
     5941 attcgcttcc cgaaccactg acttcaaaca tgacattacc aggtagcgta tcgactgcct
     6001 ctccttcatc gctaacgcca tctgcccgat cataatcaag agagaccttt gaaccgctta
     6061 agatatggat tcctggcgct gtttttccac taccagtctc atctcctgaa tctagaaaac
     6121 cgtagaattt ccctagatac ggtccataaa gttgactacc attgatcaca ttcaattgat
     6181 agcttctaca aaaataggct ttacccggag cggcattcag tccttccttc actggttgga
     6241 catccaacac accggaaaca ccatctaatg tgacagttgc acgaggagcg taaatcagtc
     6301 ctaatttgga cccatcatac aaatcaagtg tcccggtaaa ttttatttct cccgtaaaat
     6361 gatatctgct tccccaaaaa tcggtcatat aagcaatgat aatggcattt ccatcgatat
     6421 ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa attctggaac tcgatcgact
     6481 gaggtatctc attgatataa atatggtgtg tgccgaaatt gtgagcgaat ccaccaaaat
     6541 caataacgat atctcgtttg atctctaatt ccctgtctaa agtcaaaggc tgaatcagtc
     6601 ggatcttagt tactgattcg tctgcgagaa gctgcgccag ttcttctgtt gtggcagctt
     6661 cgctgtaccc atccgctgaa acagctcttg ccagtgccgc ttcactgcta tcagacgttt
     6721 gttcagctac ttctgaggtc tcacttgtct tattttcttc ttgcctttta ttagtagaag
     6781 cctcactttg agattctgta gtttcagaag taatcgattc ttctgaaaaa gtagtcttgt
     6841 caaaactttg attctctgat tgcgttgatt cgtttgtttt ctctacatcg gcaaaaacgg
     6901 atgtcaccat caagagaggg aagacaaaca aaatcgacgc aatcagcttt tttacacatt
     6961 gattcattct ccaccatcct ctctgacgtt ttgacaattt tattttatcg ttttaatcta
     7021 tttccaattg tactttcata tttaaaatat gtatttttat ggaaaaattt ttctattagg
     7081 tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca cagtccctta
     7141 tcccgaacaa gcggtgttct cccgccaact cctcggtcac aacctccatt ttaacaataa
     7201 acctgtaaat gtgagggcaa tactttgatt gtcacaggta atttatcccc ttcatctcca
     7261 tcaacattcg tcgtcaattc ttcctcatca gctaatgaca caactaattg ctcacaagta
     7321 aggtaggcta gatttttcgt agattcatct actcctttta ataggtctgg caatgtttta
     7381 agcgtatcga tcaaagaaga atcttttaaa taaaccaaat ggagcttacc atcatccact
     7441 tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc tatcagcaag
     7501 gtactgctct caatttcttc cttctttcca tcaacttcta cgtgaaacga gtaggattgt
     7561 gtggaagcta actgtttgat tcctgacatg aaataagcta gttttccaaa tttcgtcttc
     7621 ttctccgcat ctacatcatt gattgattca gggatagagc caattgcgac gacattcata
     7681 aaatagtcgt catttatttt tccaatatcc aatggcttga ctgattcaat cgaaaaatga
     7741 ttgatcgctt cctcaggttc tagcggtatc cctaatgctc gagccaagtc attcaccgta
     7801 cccaatggga aaaatccgaa atttggccga tgttcttgtt cagcgattcc actgattcct
     7861 tcgttgactg ttccatctcc gcccatgaca aacacactat gatacccttc cgtagccgct
     7921 tcgcgagtaa agttttttgc atcccctgct ttttttgtat gcaagacgac cacttcatca
     7981 aacaccgatt ccaattttgc tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct
     8041 gaactggggt ttaccactaa taaaactttt ttcatcactt tctttccctt ctgattgatt
     8101 tctttaatat aacgatagca taaagaaaaa aattaggctg tgatgaagta ctgttcatga
     8161 aacattcatt tattttttga caagataaaa catccatact atgaaactga ggaaaattaa
     8221 aacaatgata taggaaataa aagaaaatga taggcattgt atacctaata aagtaaaaat
     8281 cttaggtggc agataaaaac acaaaaatag caagagaatg taatagcgtt tcttccactt
     8341 tttgttatca gaccttatag cgatcagagg aataccaaat gcataactga aaaactcttg
     8401 atgtactgtt aatatgtcac ggccttgaat aataaatgcg acaatccccg ctataattag
     8461 tgtgcctaac accaaaacgg aaaattgatg aatatatttt atatacaaat gatccattgt
     8521 acttcctgcc tttttatttg tgagaaaaac aaatccgtat acgtgaataa cttctagaaa
     8581 taaaagaaat ttgcaaaacc actccattta tctacttcta cgtgcatttt ttgatattgt
     8641 tttctttatt taagattata cagaaaaata tctagggtcg ctggattcta agtaaaaaaa
     8701 atttttaaaa taaaaagaac taaatcaagt agtaattaaa ctactgttga tttagttcta
     8761 ataaaaagat ttagatgaac agttttattg atttaaatag ttcatatttt ttatttatac
     8821 cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa
     8881 gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg
     8941 gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt
     9001 atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat attccgtagt
     9061 ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa
     9121 ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc
     9181 gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc
     9241 tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat
     9301 ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa
     9361 tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa
     9421 tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt
     9481 ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc catatttatc
     9541 ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt
     9601 caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt
     9661 ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa
     9721 aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca
     9781 aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga
     9841 atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac
     9901 atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt
     9961 atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgc
    10021 tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt
    10081 attatgatat atttttatac aactaaaaat gaatatatta aaactaccaa aaataacttt
    10141 taacgctgtt tcattatatg ttttttctat tttatataaa cttttggata cttaatacca
    10201 accggttgga ttgtaccaac atgagaatgt gtcagttcta tttcctgtcg cacttgcata
    10261 ggcccatact ccttttgttg catttgctga tcgaatagag tttccaactt tcgcttttgc
    10321 accgtgacga ttatagacac gactatagtg gttagaatga cctgtttttc tcgtacaagt
    10381 tgtatcacta taataaaccc atcttccaga gctatgatta attgctgttc tagaaatttc
    10441 ccggtctact ggtaaatttg acttaataat attaccttta gcatcaaaat gaatctctcc
    10501 tggctttaaa gatttgtaat agatttcacc gtgaactgaa tttgcttcca cttcagatga
    10561 catagcgttt cctccaacaa aacctatccc tagtgcacaa ataataactg cttttttcat
    10621 aaaacgacat cctttccttt ttgattttta aagcatgatc ttcttgtttc ctattttggt
    10681 cagatatgga tcatgcgtga ctacgataac taacgtacct tgggaagctt gttgcgataa
    10741 gatttcaact actttatcct tatttctttt atccaatgac ccagtcggtt catcggctaa
    10801 aatgattttg ctattcttca ataagactct cgcacaggcg attctttgct tctcaccacc
    10861 agataatgtg tagataggcg tacttagctt cttgtttata tttacttttt tcaatgcctc
    10921 atactttgct gttcgcttat ctgaaacatt taaattcgaa aacttcaatc caatatctaa
    10981 attaaaatcg attgtttcat attcaatcaa cccaaaatct tgaaaaagaa aagatatggt
    11041 atttcttttt atcaagcgct tctctctccc agttaattgt ttcgtctctt gatcgaacaa
    11101 atagaattgt cctgaactga actgatcatt caatcctaat atattcaata acgtactttt
    11161 acctgctcca ctttctccaa caacagtcac aagatcccca gcatccgcag tgaaacgatc
    11221 caaatgaaga atcgttttgt ttcgaatgat ttttttcgca ccttttatca ctatcaacgg
    11281 ctccaattgt taacctccat tcatcagcaa taactgactt ttcttttctg tagacaaaat
    11341 aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa aaagatattt
    11401 aggctctctt tctataagaa aaaggaagat tacacttgtc gttccccatt tgatgaggtt
    11461 catcatgaag aaatctttat acgtttcaaa gagcgaataa ccacttgaac gaaagatagc
    11521 gattttccga caattgtaga caaaatatat gcataacatg acatagctca acaagaagaa
    11581 agaaaagaaa gtgagtaaaa atatctgtat caagtaatga aatgatctgc ttaacacttt
    11641 gacagctatc tcttcagtct gagtatacgg aaatgataac tgcaagttat cacgtaactg
    11701 actttcttca atcagctcaa ataacctttc tttcgacaaa gagcctatct tgatctttaa
    11761 cggtggatag atttctccgc tgagaatatt tctttccaaa tacatactgt ttttcaatgt
    11821 cagtatttca acaaccggat aatgttcgat ccaggggata gaaggaataa atgtataaat
    11881 gggttgattg ttttttataa atagaaattt cggttctttt tcactgattt cttgataata
    11941 ttctgttatt tgcgccaggt cattttttaa atctgtatgt ctatatcgtt cagggatcaa
    12001 tataacaggg tgcgtttctt tttcggttat ttggatcaac cttccagaga catcaatcaa
    12061 aggaaatttc ttaagataat tcagatttat ttttatccct ctgccaaact gttcattttc
    12121 ttcattcaag taatctgata cattgactaa tatacttccg tttttattca aagtttcata
    12181 taattcctga tctgctgctg atgcaaaacg attggaataa ataaaatcca cgtggttttt
    12241 cccgatggtt actggataaa atacatgata gtcctcacca gcgatttctt ttggtggttc
    12301 atgggaaaca gctaactgtg aagaagaaaa aacgatagtt gctaattcga cattggcaac
    12361 ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga atgtattgga tatagttgaa
    12421 cgcttctctt aacgaagcga ttttttcaac taataaaaga gaaatcaaag agataccata
    12481 tattccgctg aacaagaaaa cgtacatctg gaacaaataa agaaattgct ccttataata
    12541 cataaaaata aaaccaccaa tgattcctgc aattatgaat gtaataatga ttttcttttt
    12601 ccccaataaa caccacaata cttgatgtaa tgaaaagtca ttattcaaca ataaactgat
    12661 ttctttattc atcaataaga aaaaggatga taacaaaagg aataaaagaa tcaatccaat
    12721 agttaaatac actcgataat tatatacgtc tgtatttgaa ataaccgaat acgaatagct
    12781 attttcaaag tcttcaaaaa tattagtggc ataatcagtg tgaaatgctt gattgaatcg
    12841 atcagtgaat agtgaaatga cccgttcata ggcttctttt tctctcgtca tcaagaaaaa
    12901 ttcaccttca aaatctttta ttttgatcga ttcttcaatg gacgttacgc gaaaggaggt
    12961 attagaatag ggtaccttct cgtgagaaga tttgacatcc ccttgataat acaatgtcat
    13021 ttcaagcttt ggctgtatct tgaagttcca agatgtatct acttcattgg attcatttac
    13081 tgtttttttc atataagaaa gctcagtttc tgatgagaca ttttcaaata ccatgattag
    13141 ctgctcatat tcttcttgcc caataaaatg ttgtggcaat cgaatcggag tataagtatc
    13201 tgtttgatgg aaaaattcat ttgtccgata gagtacctgt ttatttatac taaccaaggc
    13261 aaaaccactc atgataccgg tcactatgat tgtgagtaaa actgtgaact tttttttgat
    13321 tatcaccact ctcccttact taaatgattc tttgataaaa tcataccgtt tttcatcaaa
    13381 aaaacgtatc tatcaagtgt ataggtcatg tagaataaat ctatatactg tagaatcgaa
    13441 aggagaagaa gatgaacacc ggagaaaaaa tacgaactta ccgcagaaat tgtaagctta
    13501 cgcaaaaaga attaggcgaa aaaattggtg tttccgataa aacgatttct agttgggaaa
    13561 attctcgtac aatgccagac tttgaaatgc tgtcactttt acatcatact ttaggtctgc
    13621 cgattgattt tccttctatt gaatcagcta ccgaatcaat agaaccaaca ttgccagatg
    13681 aacctcttgt taacaagaaa tcttattcat caaaacaacc cttctcactc aaaaagacac
    13741 tatctttctt tctgctcgtc ttttgtttgg tctatctttt tcttatcaat tatcaataca
    13801 aaaatgaaac gatcgatcga ttcaatcctt ttttgaaacc acaaatcggc tataccacac
    13861 tccctgtagg atacgtggac aattccatcg aacgttgggt atgtaatgat atctttggcc
    13921 atggtacatg gctgtctttt cacgctggag aggtagaaaa agagaaaaac catgctgtaa
    13981 tcaaacacaa aggaaaacac gtcatgaacg ttcgcctgct cacttgggaa gccattccct
    14041 cagattacca agaaatcctc agccaagact atgacgacgc cttaaaaaga agccctcagg
    14101 agccagatag gcattaagta ggagatgaac gtagagatag aaaaaatcaa tgcacatgat
    14161 cttatccaaa aaacaggggt acaaattaat tgaaaaagcc ttgagatgaa agttcctaaa
    14221 taaaaaagtt cagaatgata ggtatttctg gcttatagta taatatttac gccacagcct
    14281 tccaatgatt ctttttggaa tcataatcaa ggatggacat tcacaaaaat attttgctca
    14341 ttcgatccga atagattaac gaccaatcta gtaagtaata tagattggta cgtagtcaag
    14401 ctgcacataa tttagatcaa ttttagttct tttttatatt tctatgatct gctcatttac
    14461 ttttatatga tggcttacag taacataaat aaatatgata atcgctggta gtttatccaa
    14521 gagtactcaa taaaatacaa aatggattgt tcaagcgcct atcataatga taaaatgaaa
    14581 caaataaacg atacaaggag gaagagactt tgagtaatgg cagaacaatt ttttcattgt
    14641 aaaaattgtt cagttgttaa tctatgggat ttcggtagca tgggcatttt tttatagagg
    14701 aatcacacca acctggccgt tttatctctt gttagtggga atattagtag gaaattttgc
    14761 ccctcttcac tatcgtacga acttttttcc aactgattct ggcctttttc ttaaaacaaa
    14821 gacgcgaaaa ttagaaaaca ttattgaaat gacaattacc tgtataatca ttttcttagt
    14881 tatattccta aacgtcttct aataagaatc tcttaaaatt agcttgttac agttagttac
    14941 ctagtttata taacacagtt atcttatctt ccttatcaac atcttttgcc aactctttgt
    15001 cttaaattcg aaacatataa gcagagctac gaacaccaag actgtttcgg ctctgttttt
    15061 tgcatcaaat tcactcttat atagctgata atctcggctc tttaaaagaa attattttcg
    15121 cttcatttta ttataaaaag gctaggaacc ttgatgttat caggttccta gcctttattt
    15181 aagtattacc ccacagaccc ttccatatag attttcaaat tacacactag tttaagataa
    15241 aacaaacaag gaaaaactta ccatttactc aaaaaattaa ctcatataag tacattagaa
    15301 tgtaaaatag actggtcaaa atctggtcaa aaaagtagat gtgacacaag ttcgcattca
    15361 aagcgatgat ccatgatttc gtaatggtca attatatgga tcaaagccac gcgacaagag
    15421 cgcaatggat gtctctaccg attggttgaa taaacgaaat aaatagaatg tgaaaaagga
    15481 tcggacaaat gttccagttt gctcgatcct tttttatttg aattcatttt cgctttatca
    15541 aacatcattt gattactcag ttaaatactt ttgacatgag taccaatgaa acatttattc
    15601 attaaaatta cttatttctt atcgcctttc tagccaatgc caatccgccg attattccag
    15661 cactatcacc taattcgggc gtaacaatgt agcta
//
LOCUS       DACIRY010000058        15232 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.059, whole genome shotgun sequence.
ACCESSION   DACIRY010000058 DACIRY010000000
VERSION     DACIRY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.059"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(21..1586)
                     /gene="guaA"
                     /locus_tag="IYE22_001591"
     CDS             complement(21..1586)
                     /gene="guaA"
                     /locus_tag="IYE22_001591"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ9506804.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1645..2568)
                     /locus_tag="IYE22_001592"
     CDS             complement(1645..2568)
                     /locus_tag="IYE22_001592"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ9506805.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2764..3369
                     /locus_tag="IYE22_001593"
     CDS             2764..3369
                     /locus_tag="IYE22_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9506806.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3677..4693
                     /locus_tag="IYE22_001594"
     CDS             3677..4693
                     /locus_tag="IYE22_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9506807.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4834..6135
                     /locus_tag="IYE22_001595"
     CDS             4834..6135
                     /locus_tag="IYE22_001595"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ9506808.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6181..6843
                     /gene="deoC"
                     /locus_tag="IYE22_001596"
     CDS             6181..6843
                     /gene="deoC"
                     /locus_tag="IYE22_001596"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ9506809.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            6864..7262
                     /locus_tag="IYE22_001597"
     CDS             6864..7262
                     /locus_tag="IYE22_001597"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ9506810.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7328..8410
                     /locus_tag="IYE22_001598"
     CDS             7328..8410
                     /locus_tag="IYE22_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9506811.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8622..10184
                     /locus_tag="IYE22_001599"
     CDS             8622..10184
                     /locus_tag="IYE22_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506812.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10177..11310
                     /locus_tag="IYE22_001600"
     CDS             10177..11310
                     /locus_tag="IYE22_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506813.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11310..12266
                     /locus_tag="IYE22_001601"
     CDS             11310..12266
                     /locus_tag="IYE22_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9506814.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12365..13531
                     /gene="deoB"
                     /locus_tag="IYE22_001602"
     CDS             12365..13531
                     /gene="deoB"
                     /locus_tag="IYE22_001602"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ9506815.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13555..14373
                     /locus_tag="IYE22_001603"
     CDS             13555..14373
                     /locus_tag="IYE22_001603"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ9506816.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14390..15097
                     /gene="deoD"
                     /locus_tag="IYE22_001604"
     CDS             14390..15097
                     /gene="deoD"
                     /locus_tag="IYE22_001604"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ9506817.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         4756 a         2820 c         3424 g         4232 t
ORIGIN      
        1 gatttctagc tctcctacaa ctattcccac tccactgttg ctggtggttt agaagtgatg
       61 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
      121 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
      181 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
      241 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
      301 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
      361 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
      421 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
      481 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
      541 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
      601 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
      661 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
      721 cgatctcttg catcgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
      781 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
      841 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
      901 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
      961 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
     1021 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
     1081 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
     1141 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
     1201 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
     1261 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
     1321 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
     1381 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
     1441 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
     1501 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
     1561 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
     1621 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
     1681 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
     1741 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
     1801 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
     1861 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
     1921 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
     1981 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
     2041 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
     2101 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
     2161 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
     2221 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
     2281 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
     2341 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
     2401 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
     2461 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
     2521 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
     2581 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
     2641 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
     2701 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
     2761 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
     2821 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
     2881 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
     2941 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
     3001 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
     3061 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
     3121 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
     3181 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
     3241 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
     3301 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
     3361 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
     3421 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
     3481 ttcaaaaaga aataatagga acagagtcgt tgttttttac gataaaataa ataagataca
     3541 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
     3601 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
     3661 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
     3721 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
     3781 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
     3841 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
     3901 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
     3961 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
     4021 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
     4081 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
     4141 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
     4201 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
     4261 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
     4321 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
     4381 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
     4441 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
     4501 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
     4561 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
     4621 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
     4681 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
     4741 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
     4801 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
     4861 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
     4921 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
     4981 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
     5041 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
     5101 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
     5161 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
     5221 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
     5281 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
     5341 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
     5401 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
     5461 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
     5521 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
     5581 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
     5641 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
     5701 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
     5761 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
     5821 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
     5881 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
     5941 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
     6001 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
     6061 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
     6121 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
     6181 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
     6241 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
     6301 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
     6361 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
     6421 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
     6481 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
     6541 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
     6601 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
     6661 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
     6721 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
     6781 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
     6841 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
     6901 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
     6961 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
     7021 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
     7081 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
     7141 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
     7201 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
     7261 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
     7321 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
     7381 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
     7441 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
     7501 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
     7561 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
     7621 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
     7681 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
     7741 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
     7801 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
     7861 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
     7921 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
     7981 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
     8041 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
     8101 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
     8161 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
     8221 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
     8281 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
     8341 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
     8401 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
     8461 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
     8521 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
     8581 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
     8641 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
     8701 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
     8761 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
     8821 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
     8881 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
     8941 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
     9001 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
     9061 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
     9121 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
     9181 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
     9241 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
     9301 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
     9361 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
     9421 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
     9481 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
     9541 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
     9601 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
     9661 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
     9721 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
     9781 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
     9841 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
     9901 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
     9961 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    10021 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    10081 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    10141 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    10201 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    10261 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    10321 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    10381 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    10441 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    10501 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    10561 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    10621 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    10681 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    10741 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    10801 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    10861 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    10921 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    10981 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    11041 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    11101 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    11161 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    11221 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    11281 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    11341 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    11401 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    11461 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    11521 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    11581 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    11641 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    11701 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    11761 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    11821 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    11881 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    11941 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    12001 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    12061 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    12121 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    12181 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    12241 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    12301 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    12361 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    12421 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    12481 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    12541 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    12601 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    12661 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    12721 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    12781 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    12841 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    12901 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    12961 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    13021 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    13081 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    13141 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    13201 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    13261 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    13321 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    13381 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    13441 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    13501 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    13561 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    13621 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    13681 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    13741 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    13801 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    13861 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    13921 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    13981 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    14041 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    14101 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    14161 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    14221 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    14281 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    14341 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    14401 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    14461 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    14521 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    14581 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    14641 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    14701 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    14761 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    14821 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    14881 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    14941 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    15001 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    15061 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    15121 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    15181 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aa
//
LOCUS       DACIRY010000059        15070 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.060, whole genome shotgun sequence.
ACCESSION   DACIRY010000059 DACIRY010000000
VERSION     DACIRY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.060"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(573..1138)
                     /locus_tag="IYE22_001605"
                     /pseudo
     CDS             complement(573..1138)
                     /locus_tag="IYE22_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1317..1973)
                     /locus_tag="IYE22_001606"
     CDS             complement(1317..1973)
                     /locus_tag="IYE22_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506818.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(2541..3476)
                     /gene="whiA"
                     /locus_tag="IYE22_001607"
     CDS             complement(2541..3476)
                     /gene="whiA"
                     /locus_tag="IYE22_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ9506819.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(3510..4508)
                     /locus_tag="IYE22_001608"
     CDS             complement(3510..4508)
                     /locus_tag="IYE22_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ9506820.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(4505..5389)
                     /gene="rapZ"
                     /locus_tag="IYE22_001609"
     CDS             complement(4505..5389)
                     /gene="rapZ"
                     /locus_tag="IYE22_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ9506821.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(5524..6255)
                     /locus_tag="IYE22_001610"
     CDS             complement(5524..6255)
                     /locus_tag="IYE22_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ9506822.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(6259..7524)
                     /locus_tag="IYE22_001611"
     CDS             complement(6259..7524)
                     /locus_tag="IYE22_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ9506823.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7787..10603)
                     /gene="uvrA"
                     /locus_tag="IYE22_001612"
     CDS             complement(7787..10603)
                     /gene="uvrA"
                     /locus_tag="IYE22_001612"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ9506824.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10613..12607)
                     /gene="uvrB"
                     /locus_tag="IYE22_001613"
     CDS             complement(10613..12607)
                     /gene="uvrB"
                     /locus_tag="IYE22_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ9506825.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            12866..14368
                     /locus_tag="IYE22_001614"
     CDS             12866..14368
                     /locus_tag="IYE22_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9506826.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14370..>15070
                     /locus_tag="IYE22_001615"
     CDS             14370..>15070
                     /locus_tag="IYE22_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506827.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNER"
BASE COUNT         4362 a         3262 c         2531 g         4915 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctgagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaaattccc ttaacgagta atatttatgt
      181 acggtgattc acactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcgtttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actcctaact cctctcattt aatagttgct ctctttttta ttcagataca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt aatagatttc gattattcaa agcgattttc tattggtgga
      601 ttgtgttccc aactaacaag acggattcca aagtcattat ataactcttc aagagttaac
      661 cctttcctgt tcccaataga tgtatctctc ccacgctcga tataaaatat ggagtttcga
      721 atgccaagac ctggcaaatc tttccttgtt acagttacac tgctactaac ttccccatat
      781 aaaatccagc tcctccaaaa tgataatata tttttcgacc atcatctaac tcatgtaaca
      841 ataattgttg tgccctcttg tcactcgcca aatgcaaacg ccacgtgcga taccatgcca
      901 tgctacctgc atctgcagaa aatccatcaa ataatgcaac aattgtatct tctatcacaa
      961 tcctttcccc atctacttca tattctaatc taaaatcaaa ctcccctcgg gtaatcgcag
     1021 gtcttggtgg cttaggaccc aatattattc tacttaaaac cgcattcgtt cccagatgaa
     1081 acacagctat aacaacaata atgattaata ttttgtttct atttgataaa tttctcatta
     1141 ttcgcaaatc acctctacat tctcacatac tagttcgtaa tactccttta atatcaaaat
     1201 atctactgat cgtaataata ttcgctatga attcctacta ctcgtataag aaaggccaaa
     1261 tcatcagctt ttacttgctg atgatttggc ttgtttctat ttatcgtaat tgttttctaa
     1321 tcaccaaaat tgttaacaat cggttcagat atttcccaat tcactagcct tatattatat
     1381 tgatttaaca attcttcttc atttattgta ttttgatcaa gcggaccaaa ttgttctaat
     1441 aattctttgt catgtaaaat atcccttcta gtaatgcctc tttccctttc aatgaaagaa
     1501 gcactaggaa aagtatgatt ataaccattc aacctatctc ccatgtaata attagcaggt
     1561 cctggaaaat aaaatatttg cttattatca ctaacttcta acagcaacac taaatcttct
     1621 ccgctggcta aacgacttgt ccatctacga tatctcccta tgccttcgtt cattccaaat
     1681 ccatcaaatt caactattaa cgtgtcttct atcacatgta attcttcatt caattcatac
     1741 tcaagtctaa aaggaaattc tccatatgta atctctggca atggtggtgc tggagttctc
     1801 caaataaata caataactgt gaatagaaaa aatccaacga tcgatataaa tgttatgaga
     1861 cctgctcgaa gtaaaacccg atataataaa aatgctgtat ttcgctttgc tatatctaga
     1921 aaaataaaaa gtactgcaat cataatgata gtaaaagttg taaaaaaacg catttttgcc
     1981 ctccctttat aaacatcgat tctcaaattc taaaccaaat cttggtagtt caagttcagg
     2041 tataacgtat tccaaccata catctcaatt actatttatg aacttatgat tatcactgaa
     2101 taaatcactc catccccttg ttaaaacttc tacattaatt acacctggtt gttttcgact
     2161 agctaatcca atgaacttat caaccatcct tatccaccaa ccacgagaat cttgaccaga
     2221 agtaagaaga gcagttctca aggatgatcg agcagttcct tccagttttt taattaatca
     2281 ggccagttat agataaatac ttgatcttat ctatatcagg acttctatat taagcaaaaa
     2341 tttatgtaca aaactaaaat cttcattcag ttgtctcccc gctttttata cgattgtatt
     2401 aaaaacaata gaagcctttc caaattatgt tactccagta aagatggaat tatagtttga
     2461 cggaagaaaa gcaagtataa cttttaaaaa aaactgatac attcgcatct cctgcttcat
     2521 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     2581 attttgcgaa ttcggtgatt aattcccgat tttgatatcg cacctgaggg aatcatttcc
     2641 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2701 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2761 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2821 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2881 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata agaaatatag
     2941 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     3001 tcttggttat gctgttcata aatagaaaat atttccaaat gataacggct cgtttccgga
     3061 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     3121 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     3181 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     3241 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     3301 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     3361 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     3421 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     3481 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     3541 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttccaaaaag
     3601 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3661 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3721 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3781 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3841 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3901 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3961 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     4021 atggttttac ggtcgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaag
     4081 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     4141 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     4201 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     4261 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     4321 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc cccatcatct
     4381 gcaacagtca ctactgctgt gatatctgct ccttgatttc gcaaactttt caggataact
     4441 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     4501 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     4561 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4621 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4681 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4741 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4801 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4861 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4921 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt cgtcgtatca atgatcacgg
     4981 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     5041 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     5101 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     5161 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg taaatccact cctaaagcca
     5221 tttttgtgac tttgcctgat tctttgatta attcccagaa ttttggaatc aatcgaggcg
     5281 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     5341 ctgcaccgct catacctgtt atgatgacta attgcaaatt atctgtcata attacgaact
     5401 cctctcacat tgtgcatagt aaaattatat cattccgggc gtatcattac cagttttctt
     5461 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     5521 tttttacttt tccgatgctg tatccaatgc cttgcttctt tcagctagcg ctcgttcaat
     5581 cgtccgatct gctaaaatag attgggcatc gatcactgga taatggttat cttcagcaaa
     5641 ttcatgcatc aatgacaatt ctgtgcaacc taagataact ttttcacagt ttaggcgctc
     5701 taccgcttct tctaagattt cgtaatatag ctcttgattc aaataatccg attccttgat
     5761 ctcatgatag atcagataat tgattttttc ttgaagaaca gtatcaggaa tcactgtttc
     5821 gaatccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5881 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5941 catatgaagg ataggaatat ccgttgccgc ttgtaattct tcaaaaaagt agtgtgcggt
     6001 gttacaagtc aacacaatga agttcggctg caataaattt tgtttctcaa tatcatccag
     6061 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     6121 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     6181 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     6241 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     6301 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgaccggt ctttttcata
     6361 aaacacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     6421 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     6481 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttaaaca ctcgatcttc
     6541 tgtgacgaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6601 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6661 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6721 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6781 taacagtgga tgaccaagac acatcatccg aacttgatga tcttcatcta catacgcatt
     6841 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6901 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6961 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     7021 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     7081 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     7141 gcttacttta ttgatcagtc tttcaaataa agaataatca atatatggac agataaaata
     7201 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     7261 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     7321 gataaacaca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     7381 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     7441 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     7501 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     7561 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aactcctccg
     7621 attgctcaat tcttttttga acggattgaa cttataggaa gtttttgtct ataccaatca
     7681 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7741 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7801 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7861 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7921 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7981 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     8041 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     8101 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     8161 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     8221 ctacagcatc ttctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     8281 cttctaatgt ttcagagtta tatcgtttgc catgacatac ttcacaagga acatagacat
     8341 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     8401 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     8461 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     8521 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     8581 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8641 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8701 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8761 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8821 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8881 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcagat
     8941 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     9001 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     9061 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     9121 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     9181 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     9241 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     9301 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     9361 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     9421 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     9481 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     9541 aaagatcatc tgtcccattc aagatgatat cctgatgctc ttccggtaat tcttcgaatg
     9601 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9661 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9721 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9781 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9841 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9901 gagcatagcc gtctgccaat cgcagagccg cctctaaaga atcaaataga cgagaacgaa
     9961 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
    10021 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
    10081 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
    10141 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
    10201 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
    10261 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
    10321 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
    10381 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
    10441 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
    10501 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
    10561 tatgggcacg tgcaccgtga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10621 aagcttttaa ttctaaaatc atatctcgca cattagcggc tgtttcaaaa tccaatgctt
    10681 tcgccgcatt tttcatttct ttttctaatt tcatcaataa cgtatctttt tcttctttcg
    10741 tcatttcttc atacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10801 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10861 tgtacttctg ctgaatcgta cggcgtcttg atgtttcatc catcgctaga cgcatagaat
    10921 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10981 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    11041 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    11101 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    11161 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    11221 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    11281 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    11341 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    11401 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    11461 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    11521 ttctcgctcg gtcaccatta tacattcctc tgacttgagg catggttacg tgcgattcgt
    11581 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11641 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11701 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11761 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11821 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgcc acgtgttccg
    11881 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11941 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    12001 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    12061 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    12121 cttgtttttg gtactcaaaa ggcgaaccta atccaaaaat acatgaaacg gatgcaatga
    12181 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    12241 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    12301 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    12361 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatt agtgttggtt
    12421 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    12481 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    12541 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12601 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12661 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12721 ttttttatct tattatgcat gttacgtttt ataacataaa atgttagaaa ctcgcatttt
    12781 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12841 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12901 cttaatgaca ggacttgtga cacttgcgtt tggacatgtt gcacaagctg acaacactac
    12961 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    13021 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    13081 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    13141 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    13201 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    13261 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    13321 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    13381 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    13441 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    13501 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    13561 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13621 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13681 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13741 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13801 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13861 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13921 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13981 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    14041 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    14101 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    14161 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    14221 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    14281 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    14341 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    14401 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtgtccatc aacgaaggag
    14461 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacatttttg cgttgcttga
    14521 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    14581 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccag cactttaacc
    14641 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14701 aatctagaga agaagcagag aaaaaagcaa tcaaattgct gcagacagtt ggtctagaag
    14761 ataaaaaaga tatgtatcct gaaatgcttt ccggtggaca aaagcagcgt gtggctattg
    14821 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctaca tctgcactcg
    14881 accccgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14941 cgatggttat cgttacgcac gaaatgggct tcgctaaaga agtggccaat cgtgtcatgt
    15001 tcattgacga cggaaacttt ttagaagacg gaacgcctca acaaatcttt gaaaatccgc
    15061 aaaatgaacg
//
LOCUS       DACIRY010000060        14956 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.061, whole genome shotgun sequence.
ACCESSION   DACIRY010000060 DACIRY010000000
VERSION     DACIRY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.061"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(5..823)
                     /gene="purR"
                     /locus_tag="IYE22_001616"
     CDS             complement(5..823)
                     /gene="purR"
                     /locus_tag="IYE22_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ9506828.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(990..1814)
                     /locus_tag="IYE22_001617"
     CDS             complement(990..1814)
                     /locus_tag="IYE22_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ9506829.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(1787..2467)
                     /locus_tag="IYE22_001618"
     CDS             complement(1787..2467)
                     /locus_tag="IYE22_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ9506830.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(2490..3428)
                     /locus_tag="IYE22_001619"
     CDS             complement(2490..3428)
                     /locus_tag="IYE22_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ9506831.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(3673..4524)
                     /locus_tag="IYE22_001620"
     CDS             complement(3673..4524)
                     /locus_tag="IYE22_001620"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ9506832.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(4678..7251)
                     /gene="mprF"
                     /locus_tag="IYE22_001621"
     CDS             complement(4678..7251)
                     /gene="mprF"
                     /locus_tag="IYE22_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ9506833.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            7392..8198
                     /gene="proC"
                     /locus_tag="IYE22_001622"
     CDS             7392..8198
                     /gene="proC"
                     /locus_tag="IYE22_001622"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ9506834.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(8277..9833)
                     /gene="rny"
                     /locus_tag="IYE22_001623"
     CDS             complement(8277..9833)
                     /gene="rny"
                     /locus_tag="IYE22_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ9506835.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10244..11293)
                     /gene="recA"
                     /locus_tag="IYE22_001624"
     CDS             complement(10244..11293)
                     /gene="recA"
                     /locus_tag="IYE22_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ9506836.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(11402..12643)
                     /locus_tag="IYE22_001625"
     CDS             complement(11402..12643)
                     /locus_tag="IYE22_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ9506837.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(12883..13461)
                     /gene="pgsA"
                     /locus_tag="IYE22_001626"
     CDS             complement(12883..13461)
                     /gene="pgsA"
                     /locus_tag="IYE22_001626"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ9506838.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(13670..14554)
                     /locus_tag="IYE22_001627"
     CDS             complement(13670..14554)
                     /locus_tag="IYE22_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9506839.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         4289 a         3324 c         2602 g         4741 t
ORIGIN      
        1 cctcttattc tgaaaaataa tttcctggca caaccgtaat atttttcgtt tgagtatcta
       61 catctttgac ataaagcagt gacgtataat catcgatggc gcgatgaccg ttgaattttg
      121 attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa ttcttcaatc aagctcttca
      181 ttccgttgac tgttccgccg cctttcatga aatcatccac tacaaggaca cgtgaaccac
      241 gtttcaaact tctcttagac agttccatct tttcaattct ttcagaagat ccagacacat
      301 agttgacact tactgttgat ccttctgtta tttttgagtc ccggcgtaca ataacgaatg
      361 gcgcattcag ataataggac acagcttggg caataggtac gccttttgtg gctacagtca
      421 ttacagcatc gattttttca ccaaggtatt tagaagcaat gattcttcca acttgacgca
      481 gcagttcagg ttctcctaac aaatcagaga gataaacata tcctcctgga agcagacggt
      541 cttgttctga tagacgttct gctagagatt caatatattt tttcgcctct tcatatgaaa
      601 tttcagggat gaacaataca ccacctgcag cacccgggat tgtttccaag gttccatacc
      661 ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc actgatagaa gatttagcag
      721 attcataacg ttcggcaaag cttgttaagg aaatcaattc atgcgggtga ttcaataaat
      781 actgtgtcat gtcaactaga cgttcactgc gacgtacttt cacctattcc aactcctttt
      841 aagaaatttt atcgttttac attatataag tttttcttca aaaattcgag cattttatga
      901 aaaaataaga aaatagttcg gtatttatta aaataaaaag aagcctataa aaaatttttc
      961 gtttttataa ggcttctttt ctttataatt tagttatttt ttcttctgaa ggcaaccaat
     1021 attcgtttga tgatatttac aatcaggaac aaagcaataa agactaatgt gattgttgct
     1081 cctggaggtg tatctagata aaacgagcta gttagtcctg acagcatccc tagaaatcct
     1141 acacaaacac tgatagcaat cacgacattg aagcttttgc ctaatcgcat accgattgct
     1201 gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg caatcgggat catcacagca
     1261 atagccacac cagtaatcac attgaacatc atcgacatca aatgaacggg tagcccatcc
     1321 acgtgggcgg tatcttcatc aaaagtcaga acatacatcg gccgtttgaa taaaaagaac
     1381 aatataccga tggcaacgaa taagattcct aaaaagatga cttgctccca tgtaatggtc
     1441 acgatcgaac caaacagata ggattggata cttgttgctg aattccctcc gctcaaattc
     1501 atcaatacta acgctagcgc caaaccacca gccattaaaa tggcaatgga gatttctgaa
     1561 taggttcggt agatcgttcg cagatactcc aatatcacag ctgctaatac aacgactagc
     1621 atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta acgctacccc tgctaaagag
     1681 acatgagaaa gcgtatcagc catcagcgat tgtcttctga tcactaaaaa cacacctagc
     1741 atcggggcga ttccggcaat gaagatagcg gcaagaaacg ctcttctcat gaattcatat
     1801 gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg gatctgtctg tccgcatact
     1861 gtttgatatc ttcatgatca tgggtgatca ttagaactga tttcccatgg acatgcgcac
     1921 tatgtcggag aagacgataa aattcatttc tggattcttc atccatacct gttgttggtt
     1981 catcaagaac aaataagtca gggtcagtag caaaaatacg cgctaaactg attcgctgct
     2041 tttgtccgcc agacaattca cctattcgcc gatttcgcat atcccacatc cctacagctt
     2101 ctaaagcacg ctgtacatgt tgatgatctt tttcagagag acgcttgaac cagcggcctc
     2161 ttggatatct tcctgaacgg actaattcga tcaccgtact aggaaaacct gcattgaaag
     2221 atgcaacttg ttgaggaata taaccgatac ttaatttttt tccttcttga tttttctcag
     2281 caatctttat cattcctttt gaaggtttca gtaatcctaa cgatgctttc accaaggttg
     2341 acttggctgc tccattttct ccagttaaaa tgacaaattc acccgcatct acatgataag
     2401 agacatcttc tagtacaggt tcatcatcat agtaaaaggt caaatcagat atttcgatat
     2461 aacgcacctt acaacctcca ttctaaaact taattgatac tttcttttaa agctttcaga
     2521 tttttttcca tgacagaaat ataattttcg cctgctttga tttgttcatt agtcaagctt
     2581 tctaaaggat tcaacacttc tagcttcact cctgtttcat tcgacaatgt ctcagctacc
     2641 ttcgaagagg cattttcttc aaaataaatc actttgatgt tgtttttctt cacaaattct
     2701 tttagctcag ctaagcgact tggtgtgggt tcctgatctg gtgaaacgcc cgaaatcgct
     2761 acttgtttta agtcgtattg attcgctaaa taagcaaaag cagcatgctg agtgacaaat
     2821 gtgcggtttg ttgcatcttt tagctcatcg gagtattttt gatccagtgc ttcaagcttt
     2881 ttgatataag catcggcatt cttcgtaaaa gtttcctttt tatctggata tttttcgcct
     2941 aattcttcag caatcgttct gacttctttt atagctaata ccggatcgag ccaaacatgt
     3001 ggatcgtatt catgtgaatg accatgccct tcttctgatt cttcatgttc gtctgcttct
     3061 tgcgattcta aacggtcgat tcctttagct gcttcgatca tcaaggtctg tttgctatcc
     3121 actgaatcct ttaacgaatc cacaaaaaat tccatgtctg aactattata cacgaaaaca
     3181 tctgcatcgg aaatttctgc tctttctttt gcacttggtt cgaaatcatg cggctccgtt
     3241 ccagcaggga tcaaaagttt cacgtttcct tcatccccta cgatctcttt tgtaaaatca
     3301 tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt ttttgtctgc ttccttgttc
     3361 gtatttccac atgctgcaaa cagtaaagca cctatcatta ccgtagctgt caaaagatat
     3421 tttttcattt atatgctcct ttcaatgaac aaatcggaat tttacgattt attaacgtga
     3481 actaatgtat caaaataaaa aaacacagtc aatagaaatc catcaatttc tgatttaagc
     3541 atgtatattc ttgattaaat cgtaaaatgg atgagttgct gctcatatct agccatttca
     3601 ctacaataaa aaactgaaga ataccatttt aggattcctc agtttttcat cacctatttt
     3661 acttcttata ttttattgca gggttcgtac taaataaact tcctcacaaa aaccttttaa
     3721 cccattatat actctctgcg ctcttgaata atgttgacac aaggcaaaaa cggttggtcc
     3781 gcttccactc atcaatgcag catctgctcc gtacttgatc atacgctctt tgatttgatg
     3841 gatgactgga tgccgctttc ccgttatatc ttctaaactg tttcccattt ttttgatcat
     3901 ctgctgataa tcttgctctc ggatggcttc agcaagtccg tggatgtctt gatgttggat
     3961 ggcttgcgta tctacttctc taaacactgt acgtgtagag acactcattc ttggtttgac
     4021 caacacaacc cagcactgtg gcatagaagg taatacttgg atttcttccc ctttgccacc
     4081 gacaaatgcc gtacttccgt agacacagta aggaacatct gtaccgactt ccaaaccgat
     4141 ttctgccagt tcttctaacg aacagcccag attccataat tggttgatcc ctcttagagc
     4201 agctgcacag tctgtacttc ctccgcctaa tccagctgct actggaatat tcttcttaac
     4261 aaagatcttg acaccggttt tgatattgta tttatttttt agcaataaag cagcttgata
     4321 aacattgttc cgttcatcta ccggtaaaaa tgctttattt gtttctacaa tgattttgtc
     4381 ttctgttatt tcttcaaaaa atagatgatc tgccaagtct acgctcgaca tcaccatttc
     4441 caactcatga tacccatctt gccgtctata taatacatcc agtcctaaat ttatcttggc
     4501 tggcgctttt tcaatcaatt ccatcctctc acccattcga aaagtctaag tcgcaagtat
     4561 tctatcatag ggaaaaataa aagtatagct tatgtctgcc tagtggttga ttttgtcatc
     4621 gtttcaacag ctttcacaga attttctctt cataatccaa acaagttcta gtgattctta
     4681 taattctgtg agcacttctt ctgccaaact cggaacattt cctttttctt ttcctatcag
     4741 actgagtagc tgcatggtag caaatccgaa attacttcct ttcctataag caaaataacg
     4801 tccttcccaa tgatcgacat attttaattt cttttcataa gcagactgga aggaatagaa
     4861 aaaattacca taattatata cgatatgcat gactttttct tgaaagaaag aaaaatctgt
     4921 ttctccaaca ttagctaatg gagctaagcc tagattggct tccgagtagc ccattttctc
     4981 gtatacaata aacagattgg ctaacagata gtcagctaat tcctcagatt ctttaggtaa
     5041 gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg tgatgaagcc
     5101 aacgactttt tgatctttac ggacaatacc gacaccgcta gataatacat atgatttatc
     5161 aaatcgtccc acagagaagt atttttctcg tgcaccttga agccattttt gtgaaacttc
     5221 atttacagct tgatgtaatt cttccggcag ttgatcgtac caagtaaaat ggtatccttc
     5281 tttcatcaaa cgttgatatt caagttgctg actcagccga gacaagtctt ctgttgctag
     5341 atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa cccaattcat gaagcatcac
     5401 tacgtagctt tctgatatct tataaaacgc aagctgatag cctaaacaat ctgcttgttt
     5461 cataaactga ttcgttgctg cccgcatcat tttaggattg ccaactggat ctcctaaaac
     5521 aaagcatttg tttcctttga tctgataacc aaaaacgact tgatcttggt cgtcttcttg
     5581 gtaataaaaa taactgtaac ctgacaagcg aagctgatgg cttgtagctg tgcctccgta
     5641 tgtttccaat aaacggtcaa aacggtcttt ttcgtactca ctgccaagct tcgtagtatc
     5701 ttcagctaaa tattgataaa gcgccactaa agctagcaat gagatgccca aaccaataag
     5761 ccctgagaac cacacatcat ctgaaggaaa caaaataaaa tgatttcctg ttattcgacc
     5821 gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa acgataaaca aaaagccgaa
     5881 caagctagca tcaaagatta tagctcccca agaataaacg aattttgttc gataaaattc
     5941 ttttctggat aaccacacag ctaacagaat caagacatat accaatatca actgccaaga
     6001 taacgtacgt gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg taggcagaaa
     6061 tgcctttttg acttttactg aaagtgctct agcaaggccg agtaataaaa agccaaccaa
     6121 tagattcact gtctgatcta aaaaattgaa tgagaacggc aataatactt gaaatagccg
     6181 actgattgca gacagattag tgattgttga tagaagtacc atcatgatcc ccgcaaaata
     6241 gacagcactt acgagcacga tatgcgcaac cttttggaaa aacagtcgcg gaatgttatc
     6301 taaaaatcga ttgattttga ctcctgtatg tgtgatgaac aatagaagtc ccgtcataaa
     6361 aggtagaaca taataaaaca accgataata taatagccaa actactgcta catcttgccg
     6421 aacaggcaat tgactcattc ctaagatcat caaaacgtca aaagtcccca ttccacctgg
     6481 gaccatcgtc agcattccta tcaatgtagc aatcatgaac ataggataga tcgaaagcat
     6541 cgaaatatct attctcatca acttgccgat gatcaaaaat accgcaaggg ctcccgtcca
     6601 ttgtccaaag gatgccagta gcagaccaag caagcctttt ggataaaact cgaagaacaa
     6661 tgtctttctc tttagataag caaaaaagaa aatgactggt gctaaaaaac taccgccgaa
     6721 aagccagatc caatagcttc gaaaaatact ttctgactga ataaaaaaga catcaataaa
     6781 tgtaagaaac gaccatacag acaaaccagt cagcataaat agtgcgactt ttgatacagt
     6841 ggctaaaacc atttttctat ttgtctgctt tccataaaaa ctagcccgca gacttgctcc
     6901 taccacacca ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg cagcaagaag
     6961 aaactctttt ctttccattt ttggttttcc ttgtttttct aagactctga tcgtcaccca
     7021 atcatacaac aacatcggag tgacacccag cagtccagcc agaatcatga gcaggatcgt
     7081 catgcggctt tgttgtccca tcctttgcca aatatcttgc caactcatgc cattagcaat
     7141 atttgcgact tgatttgcca caaagactaa cacggagccg aaaaaaatca gttttaaaat
     7201 cagactatgt tcctttatcc attgcagcat caagtatcgt ttatttttca cattttccca
     7261 ttcctaactt gtatactttt gtgtcatacc ccaatgttat cttatttatt ttaatttttc
     7321 cactttagac ctgttctttg cttgcgatga tagaaaaaga aagatatcct aatgctaaag
     7381 gagaggataa catgaaagta ggatttatcg gcacagggaa tatggcacaa gctattattg
     7441 atggtttatt aaaaaaagga acagtctcga aagaagaggt gttcgtttcc agtggacatt
     7501 ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa agtaatcagg
     7561 aagttgcatc acaatcagat atcgttcttt tagccgtgaa accaaaaatc attgaaaaag
     7621 tattggaaga actaagagaa tatagccaga aagtcttatt catttctatt gcttcaggaa
     7681 aatcattaac agatttagaa acagcgcttg aagatcctca ggcctccatc gtacgtgtga
     7741 tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa aatgaagctg
     7801 ccacggagga acagcttcag caagctacga tgatttttga agcaatcggc agcgtctatc
     7861 ctttagcaga atcagatttc agtacattca ttggccttgc tggcagctca cccgcattcg
     7921 tttacatgct gatcgatgcg atgggacgga ctggtgtact gaatggtttg tccaaacaga
     7981 cagccactga gattgctgca aaagcaatta tgggttcttg tcaaaaattc ctttcaagtg
     8041 ataaaaatcc atgggaactt gtagaccaag tttcttctcc tggcggtaca actgttgcag
     8101 gtgtagttgc cttagaagaa gcaggattca tcccagcaat catcaaagga atcaatacga
     8161 cgatcgagaa agatcttgag atgttgaata aaaaataatg atagcaatgt tttttcgtgc
     8221 cttacaaaaa aagaagagct gtggcttttg ccgcggctct tcttttgtat tttttattat
     8281 ttagcataat cagttgcacg agtttcccga ataacgatta ctttgatgtg tccaggataa
     8341 tccagttctt cttcgattcg tttacgaata tcacgtacta aacgaacagc gtctaaatcc
     8401 gagatttctt ctggcttgac cattacccga acttcgcgtc cagcttgaac tgcgtaactt
     8461 gattctacac cggcgaagct gttcgaaata ttttccagac ttcttaaacg acggatgtag
     8521 ttttctaatg attcacttcg cgcaccaggt cttgctgctg agagcgcatc tgctgcagct
     8581 acaaggacag agataacaga agtagcttcc acatctccat gatgagaagc gatcgcgttg
     8641 attactactg ggttttcttt gtacttggca gctaattccg caccgatctc aacgtgagat
     8701 ccttcaatct catggtcaag tgctttccca atatcatgga gcaacccggc acgttttgct
     8761 aattggatat cttcgccaag ctctgctgct aagataccag ctaattttgc aacctcgacc
     8821 gaatgcttca agacgttttg tccataacta gttctgaatc gcagacgtcc gatgatcttg
     8881 attagatctg gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc cccgtattcg
     8941 cgaatacgtt catccatttc tttacgagat ttctcgacca tttcttcgat acgcgctgga
     9001 tggattcgtc catcttgtat cagtttttcc aaggtcatcc gtgcgatttc tcttcggatt
     9061 ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat cgatgattaa atcgatacca
     9121 gtcagcgtct caagcgttcg aatattacga ccttctcgtc caatgatacg acctttcatt
     9181 tcatcattcg gaagggtaac gactgaaaca gtcgtttctg aaacttgatc tgctgcgcag
     9241 cgttgaatcg ctaatgataa taagtttttc gctttgcgat ctgcttcttc tttggcacgc
     9301 tgttcagatt cttttaccat tagagttaac tcatgattca gctcttcttc agtcgatttc
     9361 atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag ttctttttgt
     9421 tgatcttcaa tcattttttc aacgtctttt tctcgctctt caattaattg ctgttttgaa
     9481 ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt tttctaacga gtcatcttta
     9541 cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt gagattttaa ttctaactta
     9601 ctttctctca gctcactttc aacttcagaa cggtatttct gattttcttc cttagcttct
     9661 aacaatgctt ctttttttag agtttctgct tctttttttg cactatcgat gattccagca
     9721 gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg actttgcgac cataaaccct
     9781 aaaccaagac cgacaattaa accgatgata gcgaggagaa tgttgaatac cataaaatcc
     9841 acctccatac tatctttttc atttttatca tctatacagc ttctatgata aattattcta
     9901 aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa ctttattcta
     9961 atgtttgtac cccgtagtgt caactttaaa taaacaaaga aacggctttc tacttattca
    10021 ttaaacgatg gtttttaaaa taaatgacac tagtttcata taggtaaacc tttaaattga
    10081 gaaacttttc tattaaatta ttctttgtag atgaacagat atttttattt tatgtaccca
    10141 ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca ggtcaaggag
    10201 agtacctact cttcagacca gccgtttttg tttatcataa tttttattct tctactaaag
    10261 gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca ataccataag
    10321 ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc atatagattt
    10381 tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa gcaccgcttt
    10441 tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt gagatcccta
    10501 aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg ttttttacga
    10561 ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt tctgcacgac
    10621 gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg
    10681 gattcccaaa catcacgcct actttttcac ggatttggtt gatgaaaata gcgattgttt
    10741 ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa cgtgcttgta
    10801 acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact aaagcagcaa
    10861 ctgaatcgac tactacgata tctacagccc cacttgatac taaagcatca gcgatctcta
    10921 gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc acacctaatt
    10981 tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc gttccgccat
    11041 ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc
    11101 catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg acatctaacg
    11161 caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc aatttcataa
    11221 tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag gctgctttac
    11281 gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac atttcattat
    11341 ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt
    11401 atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc gcattatatc
    11461 tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg tctttagatg
    11521 aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt ccagccactc
    11581 cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg gccatctgct
    11641 ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca atctccagaa
    11701 aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact tcagatacac
    11761 cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc gctgctgtta
    11821 cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa ttatcatcgc
    11881 cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat gctaaaagag
    11941 cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc acttcatttg
    12001 gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct tttagctcag
    12061 tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt agtttttctt
    12121 cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt ggtttcaact
    12181 cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa aaagtcccta
    12241 aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg gactgttgca
    12301 gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat tttttgtatc
    12361 cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct tttgtcaggt
    12421 catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt gtttcagcta
    12481 gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga taataaactt
    12541 cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc acgacttgtc
    12601 ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc cgctccctta
    12661 tatagtatac gctgtatttt tatatttttt tatttaaaaa gagggcaagg agctgtgaca
    12721 aaaatcttgt cacagctcct tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc
    12781 ataagtcaca atatacataa aacatgaaaa gctgtttttt gaaattatcc cttgtgactc
    12841 agagttagac ggcaggctca caacctctcc actaaacgat acttacattg atcctttgaa
    12901 cacaccgctg tttttgatga agtaatccac acctgaatag atagtaaaga tcagacaagc
    12961 ataaagcatg atttgatcta ctggaatacc gatcaggctg aatgggatat tattgataaa
    13021 cagcaagata atagcaacca tttgtgtagc cgtcttgact tttcccggcc aagctgcagc
    13081 catgacttct ccatgttcga caagtagtaa acgcaagcct gtgacggcta attcacgaca
    13141 gacaatgatc gctactaccc aaccaggggc ctttccttgg tcaaccaaca tgataaaagc
    13201 agtcataact agcattttat ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag
    13261 gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg atactagcta aagcaaaaat
    13321 aactgcacct actagctgtg tgacagccag tgtggtgtct cctacgttaa attgtcccca
    13381 atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc atacaaattc taagtacagt
    13441 taatttattt ggtaaattca cactatcgac tcctttcttc cctattctac gtgactatca
    13501 gcgaagtttc caccgatatt atttactttt ccaaatgaat ttattcaaag gagtattttt
    13561 attgaaattg atctttttga attaacgaag aaggaccgcg acacagattt gtcacgatcc
    13621 ttctttttta tacctacaag taaacagatg cgtaaactaa tttatacgat cattctgcgt
    13681 attgaagagt aaggttcaca ttttttggac tctgattgtt ttcccctgga ttaactggta
    13741 cggattctcc attcgctttg atcgttgcat tgcttgctgc accaatcgtg atggtcgcat
    13801 ttgttgctgt ttccggtaac acggtactct gtgtttcatt tgctgccagt gtcccttgat
    13861 aaacataggc attgtctact agcacaccta accatacacg attgacagca gtaaattcaa
    13921 gggttacagg cttggtagcg tctgtgatat ttactgcgat ggcactgcca gaatcttctc
    13981 ctgtagctat tgccattttc ttttcttcac ttgattttgt tgttgattct actgtagaag
    14041 actctttggt ctgcgtgctt tgctctgagg aagaggactc ctcgactgat ccttctactt
    14101 taagcgaaga agctgtgtct ccgattattg gatcagcttg acgatcttgc caagtcatat
    14161 agcccacgac aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg
    14221 tccaaaagcg acttggatgc gattcttcct gatgcattgc ttttctagaa gtacttactg
    14281 tttcaggctg cggacgttca gggaccgatc ctgtcaattt gcttttccca tcaaaaacat
    14341 cgactaaatg gtctccatct tctcctacag cttcagcgta ttgccgaata aatgcccgca
    14401 cgtaaaattt accaggcaat tggtcaaaat catcattgtc gattgcttcc aaataacgtt
    14461 tttgaatttt tgtaatctgc tgtaattcat ctaaagatat ttttttattc aggcgtgctt
    14521 gccgtaattt ttctccgatg gtttcaaaag ccactcgttc atcttctcca gtctgtttga
    14581 ttctccgaag catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt
    14641 catgtcacag ctcctttttt ccgaataaac attgttctcc tgccaactcc tcggtcacaa
    14701 gtcacaatat ccaataaata taagaagctg ttttttgaac ttgttccttg tgactcggag
    14761 ttaaacggca ggctcacgac ctctttctta aacggtgttc aatcagctgt tcctcggtat
    14821 tagctcaaaa accggcaaaa tataaggaga tatttttccg tatgtttctc taatactcag
    14881 gtcaaaacgc tgatttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg
    14941 taataagtcc aaccaa
//
LOCUS       DACIRY010000061        14888 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.062, whole genome shotgun sequence.
ACCESSION   DACIRY010000061 DACIRY010000000
VERSION     DACIRY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.062"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            252..815
                     /locus_tag="IYE22_001628"
     CDS             252..815
                     /locus_tag="IYE22_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506840.1"
                     /translation="MEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVL
                     LAYYQDYRKGWMLPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKK
                     NGNHERARATLLGSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGER
                     YYKVHDNWGDYHKVIPASWSNGTVSLP"
     gene            895..1869
                     /locus_tag="IYE22_001629"
     CDS             895..1869
                     /locus_tag="IYE22_001629"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ9506841.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2058..3194
                     /locus_tag="IYE22_001630"
     CDS             2058..3194
                     /locus_tag="IYE22_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ9506842.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            3331..3675
                     /locus_tag="IYE22_001631"
     CDS             3331..3675
                     /locus_tag="IYE22_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ9506843.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            3946..5232
                     /gene="efmA"
                     /locus_tag="IYE22_001632"
     CDS             3946..5232
                     /gene="efmA"
                     /locus_tag="IYE22_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ9506844.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5370..5969)
                     /locus_tag="IYE22_001633"
     CDS             complement(5370..5969)
                     /locus_tag="IYE22_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ9506845.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            6373..7494
                     /gene="mnmA"
                     /locus_tag="IYE22_001634"
     CDS             6373..7494
                     /gene="mnmA"
                     /locus_tag="IYE22_001634"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ9506846.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            7740..9161
                     /gene="arcD"
                     /locus_tag="IYE22_001635"
     CDS             7740..9161
                     /gene="arcD"
                     /locus_tag="IYE22_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ9506847.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            9175..10293
                     /locus_tag="IYE22_001636"
     CDS             9175..10293
                     /locus_tag="IYE22_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ9506848.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10353..11645)
                     /locus_tag="IYE22_001637"
     CDS             complement(10353..11645)
                     /locus_tag="IYE22_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ9506849.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            11795..12355
                     /locus_tag="IYE22_001638"
     CDS             11795..12355
                     /locus_tag="IYE22_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9506850.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            12484..12861
                     /locus_tag="IYE22_001639"
     CDS             12484..12861
                     /locus_tag="IYE22_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ9506851.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            12794..14866
                     /locus_tag="IYE22_001640"
     CDS             12794..14866
                     /locus_tag="IYE22_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ9506852.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         4863 a         2653 c         3228 g         4144 t
ORIGIN      
        1 tagataaaaa aattaaaaaa ttagccagca ctcagcagct gcagtcaaga aaaagtaaat
       61 tgcttttttt agctctggtt tttgaagatc aaagttatgt gattatcgat caatcaggac
      121 atccggtaga atattctcca gcaaaatata cttatcaaga gggtctttct tgtaaaaaat
      181 ggaagctgat caatgaagag ccaatcgagt tatctagatg gatcaacaga aaagaagata
      241 ttcccgtttt gatcgaagaa aaaaggtcag gaaaggaact cgcaaattgc tgggtgggat
      301 tgccagaaga acgttttttg aggtataaga aatgggctac cccctcaggc tatctttgtg
      361 gtacttacgc ggcagcagtt ctccttgcct attatcaaga ctatcgtaag gggtggatgc
      421 ttccaaatga gattcgcaag aaaaatacgg ctgattcgtt ggttttgacg aaggcattgc
      481 gtagccagat ccaaccacta ggacttccta cgattccttt tcaggttagt acagggattt
      541 ctagtttttt aaagaagaat ggcaatcatg agcgtgcacg agcaactctt ctaggttcat
      601 ggcagcgagc tacaaaacgt atacgtgaag gcaaacctgt gatgattggt attttgaaag
      661 tacttggcag tacatatggc aatcattggg tgacagcgta cgcttatttt gaaactgaaa
      721 caggagaacg ttattacaaa gtacacgaca actggggcga ttatcataaa gtcattcctg
      781 ccagttggag taacggaaca gtttcacttc cttaagaatt tgctgaaatt aacagaaaat
      841 cttggaaaaa tatgttaaac tatcgaagaa tagagaagtt ttggggagga ctacatgagt
      901 aataagtatc aggatgacac gttgaagatt tttagtttga atggcaaccg tccattagct
      961 gaaaaaatcg cgaaagtgtt cggaacagaa ttaggtaaaa gcgtcgtgaa gcaatttagt
     1021 gatggcgaga tttccatcaa tatcgaagaa agtattcgag gggatcatgt gtatatcgtt
     1081 caatcaacga atgcacctgt caatgattac tatatggaac tactgatcat gatcgatgca
     1141 atgaagcgag caagtgcgaa aacgatcaat gtcgtattgc cttactatgg gtatgctagg
     1201 caagaccgca cggcgaaacc tcatgaaccg atcactgcga aattgatcgc taacttgatc
     1261 gaagaagcag gggcaactcg agtactgact ttagatctgc acacggttca agtacaagga
     1321 ttctttgata ttcctgtaga taatttattt acgatgccgt tgtttgctca ttattaccgt
     1381 caattaggtc tgacgggaga tgacatcgta gttgtttcgc caaaaaacag cggtgttcag
     1441 cgtgcacgta gtctttcaga atatttgaac agcacattag ccatcgtaga tcatgcagat
     1501 gatgcttcaa ctgagggtgg ttatgtgatc ggtgatgttc aaggaaagac atgtatcatg
     1561 gtagatgata tcttgaacac aggagcaacg cttgcacgag cagcaaatgt attaaaagaa
     1621 aacggcgcaa aagaagtata tgcctgtgcg tcacatggat tattgtccga tcatgcgaaa
     1681 agtattttag acaatgcacc aatcaaagat atttgtatca ctgattctgt tttcacagaa
     1741 gaaaatcgtc atccagaaaa cttaacaatc gttacatgtt cagaattaat gggtgaagcg
     1801 gtcaaacgca tccatgaaaa tacaccgatg agtccgttat tccgtctgga agaaaaagaa
     1861 ttcgaataaa caaaaaaaag agggaaagaa gttgtgacag aagtgagcag cttcaaggag
     1921 ctgcgacaag acttttgtca cagctccttt tttctatgcc catgaaacgt cctctagaca
     1981 aaaatgagct cttctttggt ttgttcgcta ttttatgcta gaatgtgcct agcaataatt
     2041 tggaagggat gaatgaagtg aaaactattt atctggatca tgcggcaact acccccatgc
     2101 atccacaagt aattgaagcg atgacggcaa tcatgcaaga cacgtttgga aatccttcaa
     2161 gcatacatgg atttggtcgc cacgcgcatg aaaaattgga aacagcaaga caaatcattg
     2221 cagacagtct gcaagcaaaa cctcacgaga tcatctttaa cagtggggga acagaagggg
     2281 ataatacagc tatcttagaa acagcgttct cgcgtcaaaa agaaggcaga catctaatca
     2341 ctacagctat cgaacatcct gctgttctta ataccatgaa atatttagag acaaaggggt
     2401 ttgacgttac atatctgcct gtagatgaaa aagggaatct gtcgatcgaa gaggtcaaaa
     2461 aagcgttacg agaagatacg atcttggtct ctgtgatgta cgggaacaat gaaatcggaa
     2521 atttgatgcc aatcaaagaa atcggtgaac ttttagcgga tcatccagct tatttccata
     2581 cagatgcggt acaagcgtac ggaaaccaaa cgatccatcc agaagaatta ggaatcgatt
     2641 tattaagtat ctctgctcat aaaatcaatg ggccaaaagg cgtcggtttc ttatataaac
     2701 gagacggagt aaatatccct gcattgcttt taggcgggga gcaggaagaa aagcggcgtg
     2761 caggtacaga aaatttagca gggatctggg gtatggcaaa agcagttgaa ctgctgtcac
     2821 ctgaagaacg tgaagcacgt aacaacaaat ataaaggatt ccagcaattg attcttgata
     2881 cattagaagc aaacgaaatc ccttatcaag taaatggtga tttaacgaat aaactccctc
     2941 atgtgttgaa tcttcgctta attggcgaag caaatgattt gctactgatg aagcttgatc
     3001 ttaatggggt cgctatttct actggttcag cttgtacagc aggaaatatc gagccttcac
     3061 acgtattaga agcaatgtat ggagaagata caccgatact aaaagaatcc atccgtatca
     3121 gctttggttt gggaaatacc gaagacgaca tccagtattt ccttgatcaa ttagttcgtg
     3181 ctgtcaaaaa ataactagtg aagtcattct tatatgaaca gatgtctgag agaacgcctt
     3241 cattttttag ggaacttaac tgaaaaacct agtaaaaatc aggataaact tttataataa
     3301 agagaaacta attttatcga ggtgaaaatc atggcatttg aaacaacagc aacggtactg
     3361 ggcgcaccaa ttagctatcg tttgcatcct gatgcaaaaa gatatacatt gcgcgataat
     3421 ggatttacag aaacaaacgg aggcaattat caattgatcc gaccattaga tgccacgccg
     3481 caaagcaaag aaggattcaa attaaaaatc actgtatcaa aagatattca aacattgaaa
     3541 atgtcgatca ctacagcaaa tggattgaaa gcagtcaata ttttcaaaga tccaaaacag
     3601 aaaatgagcc aagataaatt ttattttctg atggatggga tgattagccg tgggttattc
     3661 gaaaaagtcg aatgatctgt ggatataaaa agacaactag ttttgaaacg gcctattgtt
     3721 agatgactaa ctttgtaagg acgacgcaaa ttagttgtct tttttatttt taaatttaac
     3781 acatgttgtt atttttaata ttttgctgat ttattgtttc taagtaaaga aatttaaatt
     3841 gaaacgattg ttatttaggg aaaaaggtat tttttgaaaa aagaatcccg tataatgatt
     3901 gttgtgcaaa cactatacaa aatgaaaaag aaaagaggga tgtttatgga aaatgaacag
     3961 tcggtcgttt taacgaattg gaagagaaat tatttgttct ttttatcggg acagtttttg
     4021 tcaggaatca ctagtatggt cgtccaatat gcgatcatct ggtatttgac aagagaaacc
     4081 ggttcggcga caattttgag ttttgctact ttactaggaa tgattcccat ggttttacta
     4141 agcccatttg taggaccgct agtggatcgc tgggataaaa aagctctttt gattgtcacg
     4201 gatattattg tagcgatttt cgcgcttatt ttagcagtag tcggaacgat ttcagaatcc
     4261 ttcccgattt ggctagtttt tgtgtctttg tttatgcgtt cggtcgcaca aacattccaa
     4321 atgccaacga tccagtcgat tatgccgaca atcgttccat cttctcacat aacaaggacg
     4381 aatggacagt taggaatggt ccaatcagcg aatttcatca ttgcgccagc tctaggtgcg
     4441 gctttgtttt cagtcgttcc tgtcaattac cttattttat tagatgtatt gggggcagtt
     4501 tttggtgtcg gcttattgat ttttgtgaag atcccaaaag tttctcctga aatattggaa
     4561 gttccgctta ctatttttaa agatgcaaaa tttgggctgc agcaattgat ggataataaa
     4621 gggttatggt acataacgat caatggtgca tttgtgatgc tgttgttcat gccagcaatc
     4681 agtctgtatc cattgatgac gctagattac tttggtggtt ccgttggaca ggcgggagca
     4741 gtggaagtag tctacgcagt cggaatgctc ttaggcggtg cactgatcag ttttatagga
     4801 acatggaaag accgaatgaa acctattatt atagcctaca tcattatggg cctgacgatc
     4861 ggtgcaagcg gattggttcc aaacgatagt caagggttct tgtactttct tatcttgaat
     4921 gcaggagcag gttgtgcaac gccatatttc aatacactgc tgatggcaat gatccagcag
     4981 agttatgaat cgaatgtttt aggtcgtgtc ttagggaact tcaactcttt gatgaacctg
     5041 gcaggtccaa ttggattatt atttgcagga ccattagctg atcgtttagg ggtagaaaaa
     5101 atgttcttgt tttccggaat cgggatttta ttatgcggaa tcgttttatt tttaacgtca
     5161 gctgcccgaa attatgataa agaattacag aaaaaactgg taaaagaaca ccatgagcaa
     5221 aaagacgaat agtatggaat ggagttgtga cagaagtctt gttacaactc catttctgat
     5281 cctaatgttc ctgtggcaat aagcctactc aaatgaaaaa tatgagtaat tatctttatt
     5341 tgtttaatac ttgatatagg gcagtttttt cacgacctat cgtacttcct ctttgcttca
     5401 tagatttctt tgatttgttg ctcagataac ggagtgccaa atggtagttt ttcctttaag
     5461 agttctcttt ccggtgcgtc tacgcctaca ttgacatcgt tctcgatagg tacgctgtaa
     5521 tgatggatat gtccatgaag attgcggata gtgtcgttag gcccgagcaa catcggataa
     5581 tgagtcaagt aatattgttg atggtcgaat ttaacgatgg cacctacatc ataaaaataa
     5641 aatttgggac ttccgtcagt aagcctagga tcattttttt ctaaataacg gaaaaaagcg
     5701 cgactgtcat ggttcccttt gatcaagtgg attgttcctt gcagttgagt gaccagttcc
     5761 aaaatttctg catttgcttt ttcatattgc ggatgcatag caaggtcgcc tagatgataa
     5821 acatggtcgg tttcttttat tactgcattc cagctagtga tcaactgttg gttcatttca
     5881 tccacatttg caaagagccg cggagcaaag tcgttttttc ctaataagcg ttcatggaaa
     5941 aagtgcatat cactgataaa gtaattcatg tatcttttgc ctcccttttt tcttagagta
     6001 aacaaaatca cttatctgag caactcataa catctaaaaa gaaaaggttg taaatgagac
     6061 gtgcagcttt aagcgataag aagcaatttt gaaaaatagt ttttcacaat ttataaaatt
     6121 attccttatt gccgaaaggc tggccctttt tatgagtatg ccaatttttt atatcgaatg
     6181 ctcaaaagat gagtgatttt ttggttgact atcatgtttg tgtactgaat aggtccagta
     6241 gaacagtatt tttacgactt gataattaag ggatgaaatt ttcagaaata tgacgtataa
     6301 tgtaaaatac tgaaaagttg cgaccttcaa ctcgtagatg tttcagaatc taattgaaat
     6361 gatggtgata tcatgacaga caacagcaaa acacgcgttg tcgtagggat gagcggcggt
     6421 gtcgactcat ctgtaacagc acttttgtta aaggaacaag gatacgacgt gatcggaata
     6481 ttcatgaaaa attgggatga cacggacgaa aacggtgtat gcacggccac ggaagattat
     6541 aaggatgtag cgaaagtagc tgctcaaatc gggattcctt attactctgt gaattttgaa
     6601 aaagaatatt gggatcgtgt atttgaatat ttcctagccg aataccgcgc aggacgtaca
     6661 cctaacccag acgtgatgtg caacaaagaa atcaaattca aagcattctt ggattatgca
     6721 atggatcttg gcgccgatta tgtagctact ggacattatg cgcaagtcac tcgcgatgaa
     6781 aacggcgtgg tccacatgct tcgtggagta gacaataata aagatcaaac ttacttcctt
     6841 agccaattat cacaagaaca gctttcaaaa acgatgttcc ctctaggcgg catggaaaaa
     6901 tcagaagtac gggcaattgc tgaacgcgca ggccttgcga ctgctaagaa aaaagattca
     6961 acgggaatct gctttattgg tgaaaagaac ttcaaacaat ttttgagcaa ctatctgcca
     7021 gcaaaaaaag gaaatatggt cactcttgat ggtgaagtca aaggacaaca tgctggcttg
     7081 atatattata cgattggtca acgtcaagga ctagggatcg gtggcggagg agattcgcaa
     7141 gaaccctggt ttgttgtagg aaaagatcta gcgacaaaca cattatatgt ggggcaaggg
     7201 ttccatcatc ctgcattgta tgccacaagc ttggatgcaa gtgaaatcca ctttacgacg
     7261 aatgaaccaa tgccaaaaga attcaagtgt acagcgaaat tccgttatcg tcagcaagac
     7321 gttcctgtga cagttcgctt attggatgat aatcgtgcag aagtcgtatt cgacgaacct
     7381 gtacgtgcaa tcactcctgg acaagcagtt gtattttatg atggaatgga atgtctgggt
     7441 ggtggtttga tcgaccacgc atatcaagaa acaaaagttt tacaatacgt ttaagcgata
     7501 ttaaatgaat caaaacagct atggctaaca aaagaaacac cttttctttt ggatagcata
     7561 gctgtttttt tgcaataaaa ttaattttta ttcattttat acttgaaata cttgtgtaaa
     7621 aagttcataa tatccaataa tatgaataaa gttgcttttt gttaagcaag agcagttgca
     7681 aaatagaagg gaatcttttg caagtgattt gttcaatttt ataagtgagg gtgatggaaa
     7741 tggaaaagaa atcgacgggt attagcaaat ggggactggt tgctctggtt gtcagttcgt
     7801 caatcggaag cggtgtgttt gggatcacaa gtgatctggc tagttctgca gctccagggc
     7861 cagcgatcct ttcatggata atcgttggga ttggtatttt agctttggta ttatcgctga
     7921 atcatttagg agaaaagcgt ccagatctag atggtggtat tttcggttac gcggaagctt
     7981 catttggtaa attaggtggc ttcatcagtg gttggggtta ctggttgtct gcgtggctgg
     8041 gaaatgttgc ttttgcaacg atgctgatga gcgccatggg agaatttttc ccgatattca
     8101 aaggtggaca aaatgttcct tctattttat ttgcaagtat ttttatttgg ggtctgactt
     8161 tattagtaaa caatggagtt gaaggagcga gcgtcatcaa tacaatcgtg acgatctgta
     8221 aattagtgcc attattccta tttttaatat ttgtatttat cgcatttaaa gtgaatatat
     8281 tcactgctga tttttggggt aacgtatctg ataatctagt gaatggaaca gggcagcaag
     8341 gaacgatctg gttgcagatt aaaggttgtt tgatggttat gatgtgggtc tttgtaggga
     8401 tcgaaggcgc aagtgtttta gccaaccgcg cgaagaaacg ttcagaagcc cagcaagcat
     8461 cgatcattgg tctgctttgt ttattgttta tctatatcct tgcttcgatc ttgccatacg
     8521 gcgtcttatc tcaagaagag ttagcaacta tttcacaacc tgctatggca aatattttaa
     8581 aaggaatcgt gggaaattgg ggagcagcgc tgatcaatat cggcttgatt atttcgatca
     8641 ttggttcttg gttgtcttgg acgatgctcc cagcagaaac aacgatgttg atggctcgag
     8701 acggtctgtt gccgaaaggt tggggaaaaa cgaataaaaa aaatgcccca acttattcgt
     8761 tagttgttac tgcaatcttg actaatctat tcttattgac tttcttagtt actgattatg
     8821 cctatcaatt tgcctattct ttatgtacag cggcaatttt gatttgttat ctacttgtcg
     8881 ggatctatca agtccagttt tctttaaaag aaggaatcaa aaaacaagta gtgatcggtg
     8941 ggattgctgt agtttttgaa ttgatgggca tcattttagc aggattttct tatgtccttt
     9001 tatgtagtat tgcctatctt cctggttttt gcttctactg gatcgcttgt cgtgaaacca
     9061 aacatcaaat tacgggaaaa gaaaaagtaa tgattgggct gatttgtgtg gccgcactgc
     9121 tttcaatcgg gttgcttgct gtaggctgga tcaagatata agggggaaag aaacatgaaa
     9181 atcgcaaatt tcggtgtaga agaatggcta aataaatggg aaaagaaagc ggtatatgat
     9241 attgcacaaa gctcgatcga agcattgaca ttagacgaac tcgttggctt agatgggact
     9301 agcgtaagta aatttttcga aaaacgcaaa aacgaaccat tggactatgg ctggatcgaa
     9361 ggatcagatg ttttcaaaca agaagtcgct tctctttatc aaaccgtcga tccggaaaac
     9421 atcttacaaa cgaatggagc gactggtgct aatcttttag ctttatacgc gttagtggaa
     9481 gcaggagacc atgttgtttc gatgcttcct acttatcagc aattatatga catccccaaa
     9541 tctttaggtg ccgctgttga ctttgttcat ttgaaggaag aagaagattg gcagtttgat
     9601 ttagaacagc tagaagcctt agtcaaacct gaaacaaaaa tcatctgttt gaatagtgca
     9661 aacaatccta caggcacatt gctagatcgg aaaactttag aaaaaatcgc agaaattgct
     9721 cgtacagtag atgcatatgt attgatagat gaagtatatg cgcctttaac ggataaaggt
     9781 gaattcttgt cgatcgtaga tgtctatgaa aaaggtatag caaccaattc cctatccaaa
     9841 acctactcca ttccaggtat tcgtattggc tggacagcta ctggacctga gttggcagaa
     9901 gtttttcgaa agtatcgaga ctacacgatg atttgcggcg gagtcctgtc agatgatctt
     9961 gctgtacatg cattgaagaa taaagaaaaa atattggcgc gaaacaaaaa gatcattaca
    10021 gagaaccttg tgattttaaa acaatgggta gccaatgaac cgaaagttga acttgtcgct
    10081 cccaattacg tctctacttc tttcattaaa ttagccatcg aagaagatga ccagacattt
    10141 tgtatcaatt tattagaaga aacaggagta ctattagtac caggaagtgc ctttgattta
    10201 ccgaagcatg cccgactagg ttactgttgt aaaaaagaaa ccttggaaaa gggattattc
    10261 ctcttatctg agttcttgaa aaaacaggcc taaacaaaaa cactgcaagg acagtctgtg
    10321 acaactgttc aagcagtgtt tttcatttac gcttattgtc tatgagaagg agagtgccct
    10381 gaagctatgg agactcctga agctttttct tctgcagttt cttgctgatg gatctcttgt
    10441 gtattgtgtc gtacaagcaa gaagttgacc aaaacaagaa gagaagtcga aagaaagacg
    10501 ccacgataac caaatgcact ggaaacactg gagccgatca taggccctac tacgttacca
    10561 gttgcttgga aagattggtt ataactgaag atccgtccag cagcttgatg aggcgaatat
    10621 tttgtgatca atgcttggac agcagggagc agacaagcat cagagatacc aatcaaaaaa
    10681 cgtaaggcag ctaattgcca aacattcgtg acgaatgcca ttgggatata aaccagaatc
    10741 gcaaaaccca aaccaatcgc aagtattctt tcactcccaa tcttgtcacc taagcgacca
    10801 aatcttggag cggctatcaa agtggcaatc ccaggtatcg aagcaatgat tccgctgatc
    10861 aacgtaacat tcccatgtcc atgcagcaat tggcgaatgt acagactgat gattggacta
    10921 atggaattgt tggatgcttg gatgatcatc gttgtgacga acataccgat gacgacgtgt
    10981 ggatatttca agtctttgaa aatctgtttg gcagaaacca tgttttcttt tttcactggg
    11041 acaaattttt catggacgaa aaacagactc aacaagaaga cgaaaaataa gatgatccct
    11101 gtaatgaaaa aggtagggcg atagccaaac gctgaagcag agatcccgcc aagaagcggt
    11161 ccaagtaatg ttcctgtaac agaaccagtt gctaaggttc caagaacttg accacttttt
    11221 tcttttggcg tcccagttgc gacaagggca gtggcattac tgatataacc agaaaaaatc
    11281 ccttgaagca gacgcaatgc gactaattga tacacactag tcacgcaacc catcaaagaa
    11341 atgacgattg ccattcctaa tgaagcacgc aacagcatca attttcttcc tttttggtcg
    11401 gctaattttc cccaccaagg agagataagc gtagtcacca agaaagtaga tgaaaaagtc
    11461 aatccgctcc aaaagttcaa ttgtgacgta ctataattac ctaacgtatc tatgtaaagt
    11521 gacataaaag gcatgactaa actaaatccg ataccagcca taaatgttcc aaaccataag
    11581 acaaacaagt tttgttccca agtttctctt ttcctaaata atttttcctt tattcttgca
    11641 gccaattaga ctccttcttt ctgaaaattt tcatctacag ctctttatta taaactacaa
    11701 tatgtagttt atcaatgaaa gtctcttttt tctctttctt tttcgtggta aactgaaaga
    11761 gtgaaggatt tattttatga gaggaaacat ggaaatgaaa aaaagaaatc tgtctcaaga
    11821 gaaaatcatc gactgctttc gagaactagc agaagagatg gatgttcagc aaataacctt
    11881 tcagcattta gccaaacgct tggatatcaa ggctccctcg ctgtataacc atttcaaaaa
    11941 tattcgtgat gtcaaaacag cgctaacagc gaaacttctg caggaactta atgaccagct
    12001 gcgacgggct cttgtgggaa aaagtggtca ggaagcactg cagatctatg cagaaactta
    12061 tcgatccttt gctttttcaa atcaagcagt gtatgagtta ctgatcagtg taccgcatac
    12121 caatgaagct gttttattag atggaatcca tgaaacgaat cagatcattt tgcagatatt
    12181 tgaggctttt caattaacga aagaagaaaa agttcatcga agcagggaac ttcgtagtat
    12241 gatccacggt tatctttcct tacgtttttt aggatacttt acaaaagaac caacaatcga
    12301 tccagaagac agctattttt ggatgatcaa taactttatc gctactttgc cttaaacaat
    12361 cagattcatt tgtaacaatt ttaagatgtt cattaaatga cccttttata agagaaaatt
    12421 cttgcaactt tcttgaaaag tgactaagat gagtctagaa aagctgaaag aaggagcacg
    12481 agtatgtatc aagtaattac catgttcggg gacaatgagc cttggtggtt ttttgaagac
    12541 tgggaagaag atattgaaga agaagaaaca ttcgagtctt tcgaagaagc acgacaagcg
    12601 tacgaaaaac aatggaacga aatccatcaa aattatgaat atatcaatgc aaaaagtaac
    12661 tttctcagcg ccttttggaa tgacggagat gagcgctggt gtgaagagtg tgacgatgat
    12721 ttacagcaat acaagggact tgcgttgttg aaaaatcatc agcccatcac agtagaaagc
    12781 agaaaggaat tttatgaaac aactaattct agcggaaaaa ccaagcgttg cgaaagacct
    12841 aagcaaggtg cttggtgcta atcaaaagca caaaaattat tatgaaggac ccaaggtgat
    12901 cgttacttgg gcccttggtc atttattagg attgaaaatg ccggaagatc tgaataagga
    12961 atggcagagc tggcaaatgg acacgttgcc aatgatccct aaaaaactag gaatcaaacc
    13021 acttcctaaa acaggacatc aattaaaagc aatcaagcag ctggctcagc gaaaagatgt
    13081 ctcagaagca gtcattgcga cagatgccgg aagagaaggg gaacttgttg ctcgctggat
    13141 cttagagtat gtgcatttca ataaaccagt gaagcggtta tggatctctt ctcaaacaga
    13201 taaagcaatc aaaactggat tcaagcaact aagacctgcg aaagaatatg atgcattgta
    13261 tgaatctgct cttgcccggg caaaagcgga ctggctggta ggattgaatg tgaccagagc
    13321 cttaactgtg aagtacgcgg ataatttatc agcaggccgt gtgcagacac cgacactagc
    13381 attagtgaga gaccaagaaa agaaaatcga gcagttccgt ccacaaacgt atttcacgat
    13441 cacgttgcaa gctggaaaag aacaagccaa aatgcttcaa aaaaatcaat ttgctttaaa
    13501 aacacaagaa gaagcacaaa aacttgttca attgatcagt acaagcaaag gaactgtcac
    13561 agaagtccaa gaaaaattaa aaacggaatc tgcgccattg ccttatgatt taacagagat
    13621 ccaaagagaa gccaatcaac gctatggcta ttctgcaaag aaaacattaa gtcttgtaca
    13681 aagtttgtat gagacccata agatcgttac ttatccgcgt acagacagca aatatctgac
    13741 aaatgacatg aaaagtacga tgaaagaacg attgcaagca gtcagcgact ttgctcctga
    13801 ggtcaagcag tatttgaaaa acggagccat cgtccaacag acaaaagtat tccaagacag
    13861 taaagtgacc gatcaccatg cacttttgcc aactgaaaac agagcacgtt atgaaaaatt
    13921 aagtaatgaa gagcgaaaaa tctatcaaat gattgtctct cgttttttag gactgtttgc
    13981 tcaaccccat aaagtcagcc aaacaaaagt tacggttgaa tttggtaaag aacgattcat
    14041 ttttcggcaa aatcgtgtcg tccaagcagg atggaaaggc gaaactgaaa ctgagacgga
    14101 aacagtcaaa tgggaaaaag gaatgagaat caatcccgat ttcgccatca aaaaagaact
    14161 atctgcaccg cctaaaccat tgtcagaagc aagcttgctt ggtcaaatgg aaaaatacag
    14221 tctgggaacc ccagctacgc gtgcagagat tatcgagaaa ctaatcaaat ctgaactgat
    14281 ggaacgtacc cctcaaggac tgcaagtatc tcctaaaggt aagcaattat tagtattggt
    14341 aaatccctct ttagtaacac cagatttgac agaaagctgg gaaaaagacc ttgaagctat
    14401 tgctgcagga aagaaacaag caggaacctt cttgaatgga atcgaaaaag aaacaaaacg
    14461 cttggtgaat gagatcaaat caagcaaaca agaatatcaa gatttctcga ttacccaaaa
    14521 gaaatgccca gaatgtgggg ccaatctgcg cgagaaaaac acacgtgacg gcaaaatcta
    14581 tgtctgcacc aattcagatt gcaactaccg cagaagaaaa gatccaaaag tctctaatca
    14641 tcgatgcccg caatgtcacc ggaaaatgga aatcatcgaa ggtaaaaacg gtgcttattt
    14701 ccgttgtaaa tacgatggaa cgacagaaaa attgcttgat aaaaaagagc agaagaagaa
    14761 aatgaccaaa catgaagaac gacgcttgct gaaaaaatat gcacaagcag aagaaccaga
    14821 agaaagccca ttagcagcag cactaaaagc agcaatgaag aagtaataag atatgaaaga
    14881 ggttgtga
//
LOCUS       DACIRY010000062        14289 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.063, whole genome shotgun sequence.
ACCESSION   DACIRY010000062 DACIRY010000000
VERSION     DACIRY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.063"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(90..533)
                     /locus_tag="IYE22_001641"
     CDS             complement(90..533)
                     /locus_tag="IYE22_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ9506853.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(728..2068)
                     /locus_tag="IYE22_001642"
     CDS             complement(728..2068)
                     /locus_tag="IYE22_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9506854.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2350..3246
                     /locus_tag="IYE22_001643"
     CDS             2350..3246
                     /locus_tag="IYE22_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ9506855.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3389..4330)
                     /locus_tag="IYE22_001644"
     CDS             complement(3389..4330)
                     /locus_tag="IYE22_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ9506856.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEVF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4456..5577
                     /locus_tag="IYE22_001645"
     CDS             4456..5577
                     /locus_tag="IYE22_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ9506857.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5835..7337
                     /gene="lsa"
                     /locus_tag="IYE22_001646"
     CDS             5835..7337
                     /gene="lsa"
                     /locus_tag="IYE22_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ9506858.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7404..7931
                     /locus_tag="IYE22_001647"
     CDS             7404..7931
                     /locus_tag="IYE22_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ9506859.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            8143..9204
                     /locus_tag="IYE22_001648"
     CDS             8143..9204
                     /locus_tag="IYE22_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ9506860.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9207..10103
                     /gene="murQ"
                     /locus_tag="IYE22_001649"
     CDS             9207..10103
                     /gene="murQ"
                     /locus_tag="IYE22_001649"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ9506861.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            10117..12099
                     /locus_tag="IYE22_001650"
     CDS             10117..12099
                     /locus_tag="IYE22_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9506862.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12169..12615)
                     /gene="nrdI"
                     /locus_tag="IYE22_001651"
     CDS             complement(12169..12615)
                     /gene="nrdI"
                     /locus_tag="IYE22_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ9506863.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            12826..14250
                     /locus_tag="IYE22_001652"
     CDS             12826..14250
                     /locus_tag="IYE22_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9506864.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         4608 a         2583 c         3022 g         4076 t
ORIGIN      
        1 attcacggtg ttccagaagt agcttcttct tatttcttga agttgatcac ttctggaaca
       61 tcctcttttt ttatttggat ggataatggt cattccattt ctttgatcat ttttttcatt
      121 ttgtcgattt cttcttcttg tgcctgaatg atttcttccg ctaatttttt tacttcagga
      181 tcttttaaat cagctcgttt acttgtcaag atagcaatag agtgatgagg gatcatcgcc
      241 ttcatccacg ccgtatcgtt gacagttgtt tggcttcgta ccataaataa ggaaccggca
      301 aaaacaagaa gactagcggc taaaatcata gcatttatct ttttgttttc atacattttc
      361 cacatgaaca acaacatgat gactgccatg actgcaccca tcatcaacgc catgaataca
      421 cgtgtgcgac tgaagaagat atgatcaagc gcataaacat tcaaatacat cataccaaac
      481 atgactaccg tcgaaacacc aatcatcaac aaaaacttcc aatacttttt catttaaaac
      541 aaactccttt ttcttcattc ctttcttcta tcatgtcatg agcgtggaga acaatcaatt
      601 aatctgcaca gatggttttt gaacaccgtt tattcggaaa aaagaactgc aacaattttt
      661 gtcgcagctc ctttttttca atagctccag atattcaata taaatagaat aaaattctct
      721 attggtttca ttcattcgtc aagacgaaag catcattctt tttttgataa ctgaaataaa
      781 tgaacaggta aagcaaaccg gtcaatacag ttaccccaat ggagttcgtg aataggatac
      841 caaggaaaat caaccataag actgctaatg caaaaaagct gattcctttg tcccataacg
      901 ttcctttcct tagtgtcagt acaaaggacg cagcactgat aatgatccag atcaataata
      961 ctgtatagga aagagaacct gccagatagc caaataattt gtcaccgaca aaataagaaa
     1021 gagccacacc tagataaaga acactggtac aaaacagcac agcacgttgc ggtacttgat
     1081 gcttgtttaa aacagctagt tttctagagg aacctttccg atctttcaag cggaagaaca
     1141 aaattctcga agaagcatat acacctgaat tgatagacga gaacagagca agaacaatca
     1201 cgaaattgac gatatctgcc gcaaatggaa tattcatttt ttcaaatacc atcacaaaag
     1261 gactaacgtt cgtgcctgct aaagtattcc aaggaaaaat aatcaaaagc aaaaataaag
     1321 gaataatgta aaaggaaatg attcttccca tgacgccttt gattgcttta ggaatcgcac
     1381 gtttaggatc atctgcttcg cttactgtga tagcaatcaa ctcagaaccg ccgtaagaat
     1441 agataactac aagcaaagaa ctgatgattc ccttcatacc gtggggagta aaaccaccat
     1501 gatcagttaa ttgttgaagg gtagggaaaa caccagtacc caacatttgc tgaccgacaa
     1561 gataagcagc aaaaatgatc aataggataa tgacactgat tttagcaaag gccagccaat
     1621 attctgtctc tgcgaataat cgtaccgaat aaagattgat gatagtagtt aaaacagcca
     1681 acataaaaac aaatacccaa gctgggatgt gggggaacca caactgtaaa aaggaagcag
     1741 ctgcaacagc ttcagcaatg atattgataa tccacatgga ccagtagacc caatcggtaa
     1801 aatcggcagc atgagcacct agatatggac gaatcaatcc agataatccg tggtgcgtat
     1861 caccaccact taaaacaatt tttcccagac ctttcatcac gatatataaa attatgccgc
     1921 caatcaaaaa agcgatcaat acagaagggc cggcgattcc aatcgcttcg ccacttcctt
     1981 taaatagacc tgcaccaatc gctccaccta aagctagcat ggtgatatgt cgggagctaa
     2041 gttgtttttt caatttttta tcttccataa ataacctctt tcttttaaat tcacaatcaa
     2101 aatgcatagt aagaataaat agtaagaata aatagtaata ataataatga ctaatgataa
     2161 caaattttca gataattaca actattcaga aaacttttgg gcatatttta atggaagaat
     2221 gatttatttc atcgtatttt catagaaatg ttaaattttt ataaaaataa taggaagcgt
     2281 tttactggct ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag
     2341 gcggaacaga tgaacaaaaa cgacgtattg ctaattgcga aaaataaatc actttatttt
     2401 tttccttcca tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc
     2461 attaaatcag ctcgacaaca gttggcaaat tatcagctga tcgttttttt agattatgga
     2521 tttgatatgg agatggctcg acgcgtgcgt ccatttacca atgcaaaaat cgtgttattt
     2581 ttttggaacc actttaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg
     2641 gcaatcgatg agatttatca ttttgatttt ttagaagcga aagaacttgg actaaaacat
     2701 aatagttcct tttattcgaa acatatggat ttttcttcta gcgaaccaaa gacggatcta
     2761 ttttttggtg caacaaataa cggcagaaaa gaacgggcag aaaaatataa gcaggaattt
     2821 acaaaaagag gtgttacgac caattacttt attcttccgc ttcgaggaaa tgaacaacct
     2881 ggatacctta cttatgaaga atatctgaaa aaaaccgggg aatcgagggg gattctagaa
     2941 ctgttgcggg aagggcagca aggagtgact ttgcgaacgt ttgaatctat gtattttcaa
     3001 aagaaactag tgacagataa tcaagccata gctcattatc agttttatca ttctgaaaat
     3061 atttttttgc ttcaagaacg tgatttagat gaattaccgg aatttttgca tacgccattt
     3121 caaccaatcc catcagaact gttggaattt tttgatgcag aaaattgggc aaaacgtttt
     3181 ttcactactg actccgctat ttatgaacaa tatgaatacg atctttcctc tgttaaaaat
     3241 aaataaaagt gccaaccaat catagatgaa aataaacaag cgcaattctt atggcaacat
     3301 gagaattgtg tttttctttt tgaactttca tgggggtaaa aaaatgagaa ccagctgctg
     3361 atccagctga ttctcatcag agagcctttt attctgcgcc aaggattcct tcttttgcat
     3421 ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt tgtccttttc
     3481 gccaaacggc accattgatg tagtcgattt cagtcaaacg atggtttttg atcaggtctt
     3541 ggtacataga tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt
     3601 ggtacacttc ttcttgatct aatgtgattc cttcttttgc tgcgatagaa gcaaactcag
     3661 atacaatcgt tcgaaccatg ccctctgccg ctttttgcgc acctaattca gcaatattac
     3721 aatccaatat cgtgcataat ccatttaatg ttccgtttac acaagctttt cgccaaatgg
     3781 aataacgaac attatgacta tagattggat tcaattttgc ttcttgaaag acttctacaa
     3841 ctttttttgt aaatacttcg ccttctggtt ccaagttctc tagttcgatt tctccatcac
     3901 ctaataatgt gacttttccg ggaccagcta gaccagctgt ccacatcgtg atcccaaata
     3961 agatattttt ctttggtaca tatttttcca gtacatcttc atgacctaat ccattcagta
     4021 aacacaaaac ataagtattc tcagtaataa tggattggat cgaacgaaac atatcatcta
     4081 attgattcgc ttttgtcaag gctacgatca aatccacgtg tttgttgctt tcgatgattt
     4141 cttctggtga atagattggc agttttgcaa ctacttcttt gccattaaag tcagcaatca
     4201 atccatttgt acgaatggct tcaatgtgag caggccagcg atcgatcaag acgacctcat
     4261 ttccgctttg atgcaacatt aatccgattc ggctgcccat tgctccggct ccagctattg
     4321 cgattttcat gttttttctc tcctttattc aatgagttcc cttttagtat atagaaaaac
     4381 agaagaaaat agcattgttt tgtttgaaaa aagtgaaaaa aagtgtctta cttaagaaga
     4441 aagtgaggga tatagatgga tattttggcg gcaatcgatt catttaaagg aagtgcttct
     4501 tcatttgaat taaatcaggc agttctaaat ggttgggaag accgtaaagg aagaaaaatc
     4561 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcggg
     4621 ggaacctggc gtacgattga tgggatggat ctcttatttc gtcagcgaaa aatcagttat
     4681 ttattgacga actggcaagg aaaaaatacc gcagtcattg aatctgcaca agtattagga
     4741 ttagatttac ttgataaacc aacggatcaa accatccgac ttgcttctag ttatgccttg
     4801 ggagagctta tcaaagatac actgcttcaa gaagtggaac aaatcattgt cactttagga
     4861 ggaagcggat gtagcgatgg tggtctagga ttactccaat ctttgggtgc ttctttagaa
     4921 gggtacacag aaggaaatcc tttgttatct actagaaaac ttcatttgga tcgaaggaac
     4981 gttcttgttt ctaaagtaaa agtgatcatc gctgcagacg tgaccaaccc atatacaggt
     5041 caacaaggag ctgcttggca atttggaaga caaaaaggag gaacggatac gacgttagct
     5101 ttcttggata aacaagctga aaagattgct cagcagataa aagatgtttg ggaggtggat
     5161 ttagatgaca tcccaggaag cggagcagca ggcggtattg gcggagccct ttttttgtta
     5221 ggagcgaaga tggtctctgg ttttgagttg gtcagccaaa tcactggatt agaaaaacag
     5281 atcgaacaag cggatgtcat cattacagga gaagggcgga ttgatcagca aacaatccac
     5341 ggaaaagttc catatggcgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
     5401 atttgcggaa gtagagaccg agagctggaa ggactctttg attttctccc cattgtctta
     5461 agcattcaat taggaccaat cgatttagag gaagcaatgg aacacaggca aacattagaa
     5521 aaaacagaaa tcttaggaca gacactcagc agatttttca acattctccc gaagtagttg
     5581 acaaaaacaa tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     5641 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     5701 gggagcagtg tgcagatttt cgggattggc ttggttcttt cgatgtatgg gtctttttgt
     5761 gtgccccaac ccttgcatcc tctttagcca gttctgaaaa taggactggt ctttttttac
     5821 gaggtgatgg aataatgtct aaaatcgaaa taaaaaatct gacattcggc tatgacagcc
     5881 aaggcacatt attatttgaa caagccaatc taaactttga cacacaatgg aaactaggac
     5941 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tatcctgcaa aacaaactcc
     6001 cttatcaagg gcaagtgatc catcagcaag cgtttgtcta tttcccgcag caaacaaaag
     6061 ataaagagcg tttgacctat tatgtgttaa atgagattat ggattttgag atatgggaaa
     6121 ttgaaagaga gctccaactg atgcaaacag atcctgaaat cttatggaga gaattcagca
     6181 cactatcagg aggagagaag acaaaagtcc tactggcact cttatttgtg gatgacactc
     6241 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggcaga aaacaagtag
     6301 cggcttactt gaaaaagaaa aaacaaggat tcatcgtggt gagtcatgat cggggattta
     6361 tcgatgaagt agtggaccat gttttagcaa tcgaaaagag ccaactggaa ctttatcaag
     6421 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atagctaaaa
     6481 atgaaaaatt gaaaaaagaa gtcagcaggc taaagaaaac agcagctgaa aaagcagaat
     6541 ggtctcgttc ccgagaaggg gacaaaacaa agaaaaaagt cggattcatc gatacggaat
     6601 ctaggcgagt gaataaagga gcagtaggcg ctgatgctgc acggacgatg aaacgatcta
     6661 aagcaatcgt gaatcggatg gaaacccaga tcagtgagaa agaaaaatta ttaaaagata
     6721 tcgagtatat cgatgctttg acgatgaata gccaagtgtc tcatcataaa cggctgttaa
     6781 gcgtagaaaa tcttcaatta gggtatgaaa atctgttatt cgagccagtt cattttacga
     6841 tcgaacctca tcagcggatg gcaatttcag gtcctaacgg cgcgggaaag tcatccatta
     6901 tccattatct tctggggaca ttcaacggca aggttgtagg agaaaaatcc cagcccaaac
     6961 atttgaccat tagttacgta cgccaaaatt atgaagacaa tcgagggaca ttggcggaat
     7021 tcgcgaagaa aaaccgagta gactatcaag catttttgaa taatctccga aagcttggga
     7081 tggaaagaga tgtttttcat aataaaatcg aacagatgag tatgggacaa cggaaaaaag
     7141 tggaattggc taaatcttta tcccaaccag ctgagctcta tatatgggat gaaccattga
     7201 attatttgga tgtcttcaat caagaacaat tagaacaact gattttgaac gtgaaacctg
     7261 ctatgttact agtggaacat gatcaaacct ttctagataa agtatctact gagattattt
     7321 ctcttgagaa aatctaaacc ttgtaatctc aaagacaaca aggtatggtt aaggagaact
     7381 ttgtcaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     7441 acgcacaagg attagtttgc gggcgagcag atgtgccgct gactgaaaaa ggacagatgc
     7501 aggcacaggc actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     7561 attcccctct tcaacgagca agagatacag cacaagcagt agttgatcgt ctcaatttgc
     7621 cgatgaccgc tgatgagcga ttggtagaaa tggattttgg ggattatgac ggactgccaa
     7681 gcaaagatga aaactttcaa aaagcgcgtt tagcgtttgc tgtacgtttc ccaaatggag
     7741 agtcggtctt agatgtgtac gcacggatcg ttcctttatt gaaagaatgc atggaagacg
     7801 aagaaaatgt ctacttgtta gtctgtcata atgccttgat aagagtgatc aatgcttatt
     7861 ttcatccaat gcctaatgaa gggttcttta cattcatggt agacaataca gaattaattt
     7921 cttatgagta attagacttt tgtgttattt aaataaaatg tttcaaggca ctgcttttgc
     7981 ttagcagtgc cttttttgtg gctgtgtcaa ttcttttggc attataaatc caatttttag
     8041 aaataaaatt taattatttt aacaaaagta ttgaaatttt gtttctttag atatatactc
     8101 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8161 tttatgaatg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8221 ggctttgtcg gtattttcac ttccatgcat ataccggaag atgatgtttc ggcttatcaa
     8281 aatcgattga tcgaactagg aagcgaagcg aaaaagaata aactagagtt gatggtcgat
     8341 atttcaggag aggcattgaa acgagctggc ttttcattag aagatctaac acccttgaaa
     8401 gcaattggtg taacagggtt aagaatggat tatcatattt ctaatcagca gatcgccgac
     8461 tggtcacatc aattgaaaat cagtttgaat gccagcacga tcacgcctaa tgatatcgat
     8521 gagttgaaag aagcagcggc tgatttctca cagattgaag cttggcataa ttattatcca
     8581 cgtcttgaaa cagggttgga caaagaatgg tatcaggaaa aaaatcaatg gctcaaaact
     8641 caaggattcc ttgtacaagg ctttgtacca ggagatacag agttgagagg tccactgtat
     8701 caaggattac caactttgga agaacatcga gggatccatc ctctggcagg tgctcttgat
     8761 ctattagcaa gtaacacgga tattgtttat atcggagatg caggtctgtc agaacacgtt
     8821 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8881 gatgaagttt tttacgaata tatattagga aaacatacga atcggcaaga tgatgcaaga
     8941 gatgtcatac gaagcgcaga tgcgcgtttt cgagagattc cgccaatccc agtaagaaat
     9001 acagctaagc gtatgaaagg atctattact ttagataacg aaaagtattt gcgctatatg
     9061 ggtgagatcc aattaactaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagcc
     9121 aaagtcatca aagaagaact accgctgatc aaccaaatca aagctggtat gaactatcaa
     9181 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9241 aacgcagaaa ccaagaaact tttggattag atgaaatgag tatttctcaa gcagtccaac
     9301 tcatgaacaa agaagatcag aaagtggccg ctgctgttga aaaagaactg ccaagtatcg
     9361 agccagtgat cgccaacacg attgaatcct ttaaaaaagg tggtcgcttg atttatatgg
     9421 gagctggaac gagtggtaga cttggtgtat tagatgctgc tgaatgtgta ccgacattcg
     9481 gtgtagaacc ggaaatggtc ataggcctaa ttgctggtgg agaagaagca atgactgtag
     9541 ccgtcgaagg agccgaagat tcttatgaat tagggcaaga agatctaatc aagctgaatt
     9601 taacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgattg
     9661 gtggattaga ttacgcaaaa tcgattggtg ctgttacagg aacgatttct tgcaataaac
     9721 aagcagaaat cagcgagcat gcggatttcc caatcgaagt cgatgcagga ccagaatttt
     9781 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa actgattttg aatatgattt
     9841 ctactatcag tatgatcggt atcggtaaag tattcaataa cttgatggtc gacgtcaaac
     9901 caacaaatga aaaactaatt gaacgctcaa aacgaatcat catgcaagcg acagaagctg
     9961 actacgaaac agctgaaaag tactttgagg aagcagatca aaatgtcaaa ctggctatcg
    10021 tcatgatttt gacaaatagt gataaagagg cagctcaagc aaaattagct gaagcaaacg
    10081 gttttgtaaa aggaacctta tgagggggaa aagaccatgg caaaaaacga attgactttg
    10141 gaagaactgg caaaacaaat ttatcaagga gcaggcggta tgggcaatgt tgaaagcatt
    10201 gtccactgca tgactcgtgt acgtatgaag gtcatcaatg atgatcttgt aaatgtctcg
    10261 gaattaaaag cgattccagg agtacttggc gtagtagagg atgaacagct ccaagtcatc
    10321 atcggacctg ggaaagtgaa caaagtagcg caggaaatgg ttgacttagc aggcgtgaaa
    10381 ttaggagaaa aacttccttc tgctagtgca actacagcaa gttcttctgc atctggaaaa
    10441 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaaccc
    10501 tctaaaatca aatcggtatt aaaagacatc tctaatatct ttgtaccaat gatccctgca
    10561 tttgtcggca ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagct
    10621 ggaacactag acgcaggaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10681 gggatgttta gctatctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10741 acgccaacgc ttggtggagt tatcggggct gttgtgatgc tgacagggat gaatccagaa
    10801 gcaccactga aaaacttgtt taccggtgaa gctcttgcaa ctggacaagg cgggatctta
    10861 ggtgttatct tcgcagtttg gttattgtca atcgttgaga aaaaactgca taaagtagta
    10921 cctgatgcgg tagatatcat cgttacgcca actctttccc tgattgccat cggtctgatc
    10981 gaaattttct tcatcatgcc tttagcaggg ttcatttctg acggcatggt cggtgggatc
    11041 aactgggtac ttggtgttgg aggagcattt gccggcttcg ttctaggtac attattcttg
    11101 ccaatggtca tgttcggact acatcagatc ttgacgccaa tccatgtaca aatgattgat
    11161 gaaactggtc gtaccttatt gcttccaatc cttgcgatgg ccggagcagg acaagtgggt
    11221 gcagcattgg cattatggat ccgctgcaaa aaagacaaag aattaacaga aatgatcaaa
    11281 ggggctttac cagtcggaat cttaggaatc ggcgaaccat tgatttacgg agtaacatta
    11341 cctctaggac gtccatttat tactgcttgt atcggtggag gtatcggtgg ggcagtgatc
    11401 ggaatgatcg gaaatgtcgg agcaatcgct attggacctt caggagccgc tttgattcca
    11461 ttgatctctg atggtaaatg gttaggctat atccttggtt tattggctgc ttacgctggt
    11521 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaacta
    11581 gcgaccgaaa cagccacagt aaatgaagta cctgaaaaag cagtacctca agcttcagct
    11641 gaaagtgtga ctatcttatc atcagttgct gacggaacag ttgtttcttt agataaagca
    11701 agtgacccag tttttgcaca aaaaatgatg ggtgaaggtt actttgtaga accagaaaac
    11761 ggacatatct attccccggt tactggagta ataagttctg ttttcccaac aaaacatgct
    11821 atcggcatta cgacaccaaa tggcttagaa atcttgcttc acatgggcat caacacagtt
    11881 gacttaggcg gtacgccatt cgatgtgaaa gtaacagaag gacagcaagt gacacctgat
    11941 acgcttgtag cagaagctga cttagaagca atcaaagcag cagacaaaga aacctccatg
    12001 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12061 gcgaaagcaa aagcacctgt gatggatgtc aaagcttaat aagaatagaa acaatagaga
    12121 acataaaaag aagaagccga aatttatttt tcagcttctt cttttgtgtt atttattttt
    12181 ttctgccaaa atttgataaa cgcgtgtcac atcagatggt gttccccgta attcataatc
    12241 tgccaagaaa ggaaagccga atctttgtgc gtattgtttg gctgtcagac aatattgatt
    12301 gttgaagttt ttattaccgc taccgactac accaaggcaa agtttatgat tgtcttcgta
    12361 ttccagatat tcgcgcatcg tttcggtcaa gatttctgtg tcaccgttgt ctaacccatt
    12421 cccgccgtct aaataagtag gaacgaaagt aaaaaatggt tctgtttctt tcgtaaaatc
    12481 actattttca tggatttctt ttaaagtaac ttcaggattg acaggatctt ctgcatgcat
    12541 tttttcagca tattctgcta gatgtttggc aaaagcacgc gtatttcctg aaatcgatat
    12601 atataaaatg ttcatggatc atcccccaat tctttccctt tagtatagca tctttttaag
    12661 tttttttggt ggtgtaattt ggaaagggac taggcgcttt gcttgtataa tgagaaaaaa
    12721 gtcggtagaa ttatatttct attaaagttt ttattaatta agtaaaaata aaaaaggttt
    12781 gttatacttt ttgataaaag cggttttact ttggaggatg cttacatgga tacagagcaa
    12841 cagaaaaaag gaattggttt gattccactt gctgccttag tgattggttc agcgattgga
    12901 ggtggcgtat ttggtattat gacggacatc tccggatcgg ctggtggccc agctatcttc
    12961 agctgggtac tcgtaggaac agcaatgttg atgctctcct tatcgatcaa taatatcaat
    13021 aaaaaacgtc cagatttaaa aggcgggatc tttagttacg cagaagaagg ttttggccgt
    13081 ttcgctggtt ttatcagcgg ttggggttac tggctcacat gctggttagg aaacgtggcg
    13141 ttcgcaacat tgttgatgag tgcattaggt tatttcttcc ccgtattcga aggtggtcaa
    13201 aatgtggcat cgatcgtgat gagtacgatc ttcttatggg gatatgcatg gctagtgaat
    13261 cgtggagttg aaaatgctag ctttgtcaat gctatcgtca cattttgtaa attagtgcca
    13321 ttatttttat ttattgtagt agctatcagc accttttcga tccaacaatt cattaataac
    13381 ttttctagcg atatcatatt gaatgcgaat gggcagccga tcccgttttc tagtcaagta
    13441 ttgagttgca tcatggtgat gctctgggta ttcgttggta ttgaaggagc ttctgtcgta
    13501 ggttcacgag caaaaaaaca atcggatgtt gggaaagcaa cgattctagg aatattcggt
    13561 ttgatcatta tttacgtctt ggtttccatg cttccatatg gcacaatgac gatgaatcag
    13621 ctgcaaacga tcaaagcgca acctgctatg ggatatgtat ttgaaatgat ggttggtaaa
    13681 tggggggcgg tcgtgatcaa cggcggtttg atcatttctt tagttggggt ttggctctca
    13741 tggacaatct tgccgattga aacaatgggg aatatggctg aggacggact tttgcctaaa
    13801 gcatggggaa aactcaacaa aaatggggca ccaacttatg ctatcgtatt gacgacgtta
    13861 tgtacgaatg tctttttact ttcattatta ttcactgaca gtgcatataa ctttgcttat
    13921 acacttggta cagcagcgat tttcttcacg tggcttttct tagggctgta ccaaatgaag
    13981 ctttcttatc agcggaaaga gtgggtccaa tttttagtag ggtttttagc aagtgctttt
    14041 caagtatggg ccatgctagt tgtagccttt aatgaagtga tgcttgttct tgttcttttc
    14101 ttaccaggta tttattttta cctcaaagca cgtaaagaac aaaacgaacc tgtacaacat
    14161 atccacttag ataagatttt acttatcggt gtgacgatgg tcggagtaat cgcaatggtt
    14221 ttatttgcca ctgggatgct tcacgtatag aagtataaag agaggctgtg aagcctgtgg
    14281 tttattcaa
//
LOCUS       DACIRY010000063        14242 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.064, whole genome shotgun sequence.
ACCESSION   DACIRY010000063 DACIRY010000000
VERSION     DACIRY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.064"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(174..1472)
                     /gene="asnS"
                     /locus_tag="IYE22_001653"
     CDS             complement(174..1472)
                     /gene="asnS"
                     /locus_tag="IYE22_001653"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ9506865.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(1512..2702)
                     /locus_tag="IYE22_001654"
     CDS             complement(1512..2702)
                     /locus_tag="IYE22_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ9506866.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(2723..3250)
                     /locus_tag="IYE22_001655"
     CDS             complement(2723..3250)
                     /locus_tag="IYE22_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9506867.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(3297..6086)
                     /locus_tag="IYE22_001656"
     CDS             complement(3297..6086)
                     /locus_tag="IYE22_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ9506868.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEMELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(6233..6433)
                     /locus_tag="IYE22_001657"
     CDS             complement(6233..6433)
                     /locus_tag="IYE22_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9506869.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(6885..7205)
                     /locus_tag="IYE22_001658"
     CDS             complement(6885..7205)
                     /locus_tag="IYE22_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506870.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(7589..8707)
                     /locus_tag="IYE22_001659"
     CDS             complement(7589..8707)
                     /locus_tag="IYE22_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506871.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            9178..10485
                     /locus_tag="IYE22_001660"
     CDS             9178..10485
                     /locus_tag="IYE22_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ9506872.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            10604..11803
                     /locus_tag="IYE22_001661"
     CDS             10604..11803
                     /locus_tag="IYE22_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ9506873.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(11830..13098)
                     /locus_tag="IYE22_001662"
     CDS             complement(11830..13098)
                     /locus_tag="IYE22_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ9506874.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(13261..13908)
                     /locus_tag="IYE22_001663"
     CDS             complement(13261..13908)
                     /locus_tag="IYE22_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ9506875.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         4074 a         3005 c         2575 g         4588 t
ORIGIN      
        1 cacaaaattt ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg
       61 gtattcaaaa gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg
      121 tttttgcttg gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga
      181 taaattcggt tcaacaaacg tgggaatgga atagcttcac gcacatggtc gataccagca
      241 atccaagtga ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat
      301 ttacgtagat caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt
      361 tctagcaaga aatcatagtc cgttgcccgt tcgcttccgc caataatttc accgtaacct
      421 tctggtgcga tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg
      481 tagaatggct tgatagcttt tggatagttt aggataaaga caggctgttc gaatgaattc
      541 gcaatgaacg tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca
      601 tttttcttca gtaattcgat cgcatcatca taagtaatac gagggaatgg caattttgta
      661 taagttttta agacttcttt gtctcgtcct agtacatcta atgcgtattc gcaatgatct
      721 aaaacatttt gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga
      781 tgagtgaaag ccatttctgg ttcgatcatc cagaattcaa tcaaatgacg tcgtgttttt
      841 gatttttcag cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca
      901 gcttccatgt ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt
      961 tcgaatagat ccgtagtgcc ttctggtgcg gaaccagtca agatcggcgg atcgattttg
     1021 acaaaatgat gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc
     1081 ataatcgcat gttgtcttga agaacgtagc cataaatgac gatgatccat caagaagtct
     1141 gttccgtgtt cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt
     1201 gtaattccga tttcataacc aaattttgaa cggctgtctt cacggatttc tccagttact
     1261 aacacggatg tttcttggtg cagattttta gctgtttgga aaacttcttc cggcacttcg
     1321 ctttttacaa ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt
     1381 tttccgcttg atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg
     1441 tttttcgaat cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa
     1501 tttcttcagg attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct
     1561 tcttctaacg tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag
     1621 ccttcgccag tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg
     1681 acattttcat atttacacat gcgtagcgtt tcttttacat taggaaaaag ataaaaagct
     1741 ccttgtggct tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc
     1801 aggcgttctt caaaggcgtt ccgcatttct tccactgtat cttgctcgcc tttcaatgct
     1861 tcaacagctg catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg
     1921 atcattccat tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc
     1981 gcatatgttt tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc
     2041 gttgcaattg gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca
     2101 actatcaaaa tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta
     2161 taaatcattc cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa
     2221 cgtgcttgtt ctagctgctc aacagtaact ttccagctat tggattcttc tcctttcaaa
     2281 aagacaggaa gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca
     2341 ggaatgatga cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat
     2401 ttcgctccgt ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc
     2461 agataatcaa cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta
     2521 gctttcccat tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa
     2581 tccggttctc ccacagtcaa actcagcaca tctctgcctt tggcttttaa tgccttggcc
     2641 tttgccgcag ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc
     2701 atcccattca ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt
     2761 caaagccaat caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca
     2821 caggctgatc atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg
     2881 cagcaattgt ctgttttgct tcattttcag tcagtccctt tgtttgatcc aatactttta
     2941 ctttttcacc agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg
     3001 tcagtgaaaa gtatgtcttc tttcgagtaa accaataaaa atgatcggct tcctcaagat
     3061 ttgcatattt ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg
     3121 aacgcaagta aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa
     3181 gtaacacgcg attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct
     3241 ttatcttcat tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa
     3301 cttccttctt catttttctt taagaattca tggatatcct gatagatttc ctccatagct
     3361 agctctttga tcggtacttt tttcggtaat gcatggatga tccgtgcacc atattttgca
     3421 gtaactagtc ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag
     3481 cgtcctaatg cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga
     3541 ttgatcccct ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta
     3601 tcgaagggta aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct
     3661 tcccaaaaac tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa
     3721 agcttctccc ggcttccgcc aaacccttga gctagtattt ctcgtccttt ttctagcata
     3781 gacagatgca ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt
     3841 attggccgat cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat
     3901 gcttgattgc tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt
     3961 ttctcataat cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagccgc
     4021 ttagagagat agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga
     4081 tcatatcgag gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga
     4141 aaaatcatct gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag
     4201 tcttccagcc attgatccat cagcaagtac tgcttgtcta attgatcaaa taagctatat
     4261 aaacttgcta aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc
     4321 agacgcattt gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg
     4381 gctgtcccag attccagtgg cagctgatcc aacatctctt ttgtcgtcat gacttcttct
     4441 ggatactgtc cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa
     4501 atctgtgctt cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct
     4561 tcatgttctt tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat
     4621 tgctggattt gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg
     4681 atttccggca aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt
     4741 tcttttcgct gtgtttcttg tattaaaaac gcatgattga caatcaaaaa attactttgc
     4801 ctgattttct tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct
     4861 aaaaatccag tgcctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa
     4921 tgtaattcat ccaaatcacc ggtcttcgtt tgcgtcagcc aaactaagac cgccatttga
     4981 tacaaagcat attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgtaaa
     5041 tcaatatagt ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga
     5101 ttcagcaatg gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg
     5161 atgacagcgg gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt
     5221 ttacccattc ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg
     5281 tcgtagaccg cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac
     5341 attttttctt ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacgtatatg
     5401 aatagttcca tttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca
     5461 agtggctctg gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc
     5521 atacctgtgt gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc
     5581 atccgttctt cgatcagcaa aagaagttga cccgtcactt cggcatcact atctgcctga
     5641 tgaggattct catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt
     5701 tccgtcggca agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta
     5761 ggaagtccgc atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc
     5821 gcgacaaata tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa
     5881 ggcgcttttt ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc
     5941 ttccgccctg gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg
     6001 acgcagccaa attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca
     6061 acgactgcat aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt
     6121 ttgaaattat actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca
     6181 aaaacgccca gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt
     6241 tagtaacgtt tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt
     6301 ggccttcttc taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat
     6361 cgtttccgtt ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg
     6421 tacctgtttc cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga
     6481 atggtgtata tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa
     6541 ctactcctat agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat
     6601 taaaaaaagt tttctcagcg attacataaa gtgaaaaacc gaactttttt taaaataaac
     6661 gacatattgt tcagttggtg tttttttgct ctgatgttct tctttaaact aaaaaaatta
     6721 ttctttctga ctacttatcg gttctgtttc aacggtcgag gctggggaag attctttagt
     6781 aggtctgatg ttctcatggt atttctgata gcctatgaca gttaccagaa gaacaaggca
     6841 aaccaatacg atcaacgaga ttagtttttc ttttttgctc atatctatac ctcataaata
     6901 acaggatgat gtccatcata tttctgagta tgagtaaaag caaaagctgc agctgtagaa
     6961 tgtaagaaat gccagcaact ttcataatca caggtcggac agtaccaaaa gtaaccaaat
     7021 cttggctgca aagaccgttc ttctgtttca ggaatcgcag aatcaagatc tgttgtctgt
     7081 acataaacat ttttgtacag acgtgtgcct gtaataggag accgctgtgt ttcttcagca
     7141 aaaactggct gacttgttaa acttagggat agaaacagca aacaccataa cagtcttttt
     7201 ttcataaaga cctccttatc gataagtttt tcccaattaa aatcatatct caagaacgcc
     7261 cgttattcac attttgttca taaatgatgt tttttgtatc ataatcgtca aaaagaggtc
     7321 gtgaccacgt cacaacctct tttttaaacg gtgttccaga agtagcttct tcggaaataa
     7381 gtcgaaattt cacaaaaatt tgagaaataa tttttgtcaa tctcttctta tttcttgaag
     7441 ctgaccactt ctgtcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
     7501 ttagctcaaa aaacggaaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca
     7561 ggtcaaaaca ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt
     7621 catctggcgc atctttttga tccaaaataa acactttccc gccattaatg atcacatcat
     7681 ctgctaatgc attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac
     7741 catcagctaa gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg
     7801 gaacgatatc aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg
     7861 attcttgagc tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg
     7921 atagctgcgc aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa
     7981 tttttttgaa catcgtctga ttttctggta atgcaaataa ataaatcggc cattgttcag
     8041 gattttcgaa agaatccttc aagtacgtat caacagcttg gtaatagttg acccaatcga
     8101 tctctacttc ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc
     8161 ctcctccatt agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa
     8221 cgacatcaac cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgacct
     8281 catacaattt catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc
     8341 catttttgat aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg
     8401 ggatactcaa acgatggata taggtctggt cagcagttat aatgaccgct acactcgtag
     8461 ttgcactacg ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg
     8521 accagtcatg ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt
     8581 tgaatttgat ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg
     8641 tgacgaatgg tcctttagcg ccttctgaag tcaattgtgt caataattct ttatcctttt
     8701 ttgccataaa cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca
     8761 caacactatt tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt
     8821 taaatttagc tattttttta agttatcttc ataatttcct caattattta gccccagtct
     8881 tcaactattc ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta
     8941 tgcatttgat tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt
     9001 cccgcttatt agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct
     9061 aaacttccat aacgcgcgtt cttatcctaa agttttttca gaaacattga aactctctcc
     9121 cctctctgat aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg
     9181 tccataaaaa aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct
     9241 tttctaccag ctgttgctgt gcaagctgaa gatacgttca aagtaaatgc aaaagcagct
     9301 tttgctgtag atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg
     9361 gggatcgctt caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa
     9421 ggaaaactgt catgggatga taaagtttcg atttcagatt atgcagaaaa tttgagtatc
     9481 accccagatt tatcgaatgt tcctttgcat aaagaaaaca cctacactgt caaagaatta
     9541 ttcgactctg caatcatcca gtcagccaat gcttcaatgg tggccttggc agaaaaaatc
     9601 tctggcagcg aagctaaatt cactgaacga atgaaagaac aattgaaaga ttggggaatc
     9661 aaagatgcaa caatcgttaa tgcatcagga ttgaacaact cttatcttgg cgaaaaccga
     9721 ccagaaggca ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt
     9781 gcccgccatt tgatcttgga tttcccagaa atactggatg tatcaagtac tacgacccaa
     9841 atgtttggcg aaaatacgca atcaccagtg gaaatggtca actggaactg gatgctccca
     9901 ggattcgtta attacaagga aggcgtggac ggattaaaaa ctggtacgac tgagcttgca
     9961 ggtgcttgtt tcgttggtac gatcactaaa aacgatcaac ggatcatcac tgtcgtattg
    10021 aatgcggacg gccatgcgga aaacccgagt gcccgcttca atgaaacaga ccgtttgatg
    10081 gactactgct atgacaattg gtcagaaaaa gagctaggaa aagctaacgc aagtattcct
    10141 agtctaaaaa ccattgatgt aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa
    10201 agccctgtaa aagtatgggt acgaaacgat atggatactg gcaaattaac gatcacgcca
    10261 acacttgaca agaagcaagt cactgacaat gagatccaag cacctatgga taaaggtaca
    10321 aaaatcggta ccgctacgat caccttggca gacgataaat taggctattt agaagacaac
    10381 aatgcacctt caacagagat cattacggat aaaacagtgg aaaaagcgaa tatattcgtg
    10441 attggctggc gacgcgtcgc agaatttttc ggcaacttgt tttaattgaa caataagaaa
    10501 aacatctttc catcggaaag atgttttttt gctttttcta gtttaaataa cagctttttt
    10561 catctatttt cgttatactg tataagaaaa aggagttgaa tttatgaatc taaccagaga
    10621 tctgatcttt ttagttctct atcttattgg ggtattctct atcgtttatt ggcgaaaaca
    10681 gaagaaggat ccttccttgt tcttttacgg cagtacgtgg acatttacgg taatcttaac
    10741 ttattttgtc gtgttggaaa ttttttattc gaccttttat tttttgattc cattgttgtt
    10801 ctttagtatc tttgcttttt cttatttcac agaaaaacga aggctgctga atggtctttt
    10861 attcaatgtt ttcctcatca gtttcggcat ctacttgttt gtcttgctgt atgaaacgca
    10921 aaatattttc cttggcggac tgatcgcctt gatcacgatt ccattgctac ttgtcctttt
    10981 atttggtatt tatggattga ttgtttttct tttttggaac ggggtcacag ttttacgtag
    11041 agaatcgcat tcattagcta atcttttaac gttgatacta gccatttttc ttacactgtt
    11101 tcttgttttt gactttttct tgttaaaata tttgccgcaa tggataaatg ctttgttttt
    11161 ctgcgttccc ctgatcttga tctatctatt tatcgtattc tacaactttt tgactgtctc
    11221 attcttgtat cagttcaacc ggcctcgata caatcaagac ttcatcgttg tgttaggcgc
    11281 tgggttgatc aatggagaaa cagtttctcc tcttctagcc aagcgaatca ataaggcaat
    11341 tgctttttat cgggctcaaa gccgtgctac attaaatccg cccatcttat tgatgtctgg
    11401 cgggcaagga gcagacgaaa aagtaccaga ggccattgcg atgaaacaat atgccatgga
    11461 acaaggaatc cctgaaagag acattcttgt cgaaaccaat tcgacaacca ctttggagaa
    11521 catgctctat tcaaaggaaa tcatggatca gcaaatgaaa gggccatacc gcgcgatctt
    11581 tagttccaac aattaccaca tttttagagc cggtctttat gcgtggcaag ccaaattgaa
    11641 agcaaatggt attggtgcaa aaacagcttt ctactacttg cctaatgcgt ttttaagaga
    11701 atacatcgcc atcttgatga tgaacaaaaa aagacatatg atcatttgcg gattgctttt
    11761 cgcaggctcc gcattcttat cgctgattac tttacttttc taaaaaaaca aggtgtgaca
    11821 aaacatatgt cacaccttgt ttttttcttt cattctctta actattggca aaaagagtat
    11881 cccttttttg accgctactt gctgagcaga ataaataccg ctgttgcctg aacacataaa
    11941 gctgatcaag cagatcataa agaagtatac tgccgcttgt gaaccgaaca attcgatccc
    12001 catcacaaag caggcaatcg gtgtatttgt cgcaccagaa aatactccga taaagcccaa
    12061 tcctgcaaga aaaggaacag aaacatggag caaaggcgct agtgctgcac caagcgttgc
    12121 accaatttca aacaatggtg tcacttctcc cccttgatat cccgcgccta aagacaaaac
    12181 tgtgaagaat aacttcccaa taaaatcata gacatgtgcc tgtccattaa atgattcttg
    12241 aagtaacgga agactcaatc cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac
    12301 aactacagct ccacctatcg tatttcgcca aacaggatga ggaatccatt gtgcatatcg
    12361 cgcttttaaa aagacgatcg aacggctgaa tacccaacca atcaaaccaa aagcaatccc
    12421 agcaacaaaa actttgaaga acagcaacat actccattca ggaatcacac ccatttggta
    12481 atgtagatga gaaacaccat agctttctgt catgaaattt gcaaacagtg cagcaaagaa
    12541 gctagggaag atcgcttctg ttcggacttt cccgattgcc aacacttcta atccaaataa
    12601 tgttccagct aaaggtgtgc caaatacaga actaaaaccc gcactgattc cactgataat
    12661 caagatttcc cgttctgatt tatccaatcg gaacaaacgt gctgtattat ctgctaacgc
    12721 tccccccatt tggactgctg ttccttcacg gccaacagat cccccaaata aatgggtagt
    12781 tatggttcca aacagtgtaa gtggaataag tcgcaacggg attttttctt ctccaccatt
    12841 tccttgatcg atcactaagt tgtttccacg actagaaaga ccgccgtgat acgcatagag
    12901 ataagcaaaa agcgcaccac taactggtag tagataaagg agccaaggat gtgatagtcg
    12961 gatatctgtc acaactgtca ggctatttaa gaaaaaagca gacaagctcc ccatgaatat
    13021 accgagaatc gctgtgatca ccagccactt cagcatgtac actaaaatgc gtactggact
    13081 cggtaattgt ttttccataa aaaattcctc ctcaataaaa aaacgcctac taaagaaagg
    13141 ggctgtcccc agactttagt aggcgtcatt agctatcttt cgttagcatt taaaggcgaa
    13201 caccatcacc tattcacttc cattaaaaga tagcatattt ttctgtaaaa aacaataaaa
    13261 ttatcgttct ttctttacac gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg
    13321 ttgattacat tggtcactat catgacaaat ataattcccc tttttcgtat acgtgccatc
    13381 agaacctgct ttcgttgtag aaagaaaaag cgaaacattc gatgttttct gacagattgc
    13441 acagattccc ttatttatcg ttggtgataa agtaccacta atccctttta atttttcatc
    13501 ttcgtaatag atcatgaatt ttttctgcgt acctggatca ttccagccga tgtacgtatg
    13561 ttcttgcaag tcgatttcat tccaagctgg ttgtttgagt tttttcactt ttcggaataa
    13621 tttctcgatt tgtggtttgc taggctgcat aaacggctga acgatttgct tcagctcctc
    13681 caatgctcgt tctgcttgcg cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt
    13741 atcgatttct ggcagttcgt gttctaatat gtctcgaatc ttttcagttg ctagtgcctg
    13801 aactgtttgt accgtttttg gatcattgac agatgagtac gcatttaata actgttctac
    13861 ttctcttttt ataaaaaaat attggtatgg ttttattgtt tgttccatga ttttaaagtc
    13921 ctttcttcta accaaagtgc atactttgta gaaaaaagtg atcttctgca aagtatgtgt
    13981 tacctgaaga tcccggactc ttttaaataa gatacgtttt gcatcttatc atcaccccct
    14041 ttaatgctta ttcttctatg tacatgattc attcgataaa taaaagtaaa ccgtatcttg
    14101 aacagatagt caactgcaaa cagaaaaaag gactgtgaca aaagtcgtgt caaagtccct
    14161 tgtttcgaat gaatggtgtt caaaagcttg catctgcggt aataagccga aacttcacaa
    14221 aaatttgaga agtaattttc gt
//
LOCUS       DACIRY010000064        13886 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.065, whole genome shotgun sequence.
ACCESSION   DACIRY010000064 DACIRY010000000
VERSION     DACIRY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.065"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(176..1462)
                     /locus_tag="IYE22_001664"
     CDS             complement(176..1462)
                     /locus_tag="IYE22_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ9506876.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IYE22_001665"
     CDS             complement(1455..2723)
                     /locus_tag="IYE22_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ9506877.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IYE22_001666"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IYE22_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ9506878.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IYE22_001667"
     CDS             complement(3495..3941)
                     /locus_tag="IYE22_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9506879.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="IYE22_001668"
     CDS             complement(4034..4279)
                     /locus_tag="IYE22_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506880.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="IYE22_001669"
     CDS             complement(4394..4972)
                     /locus_tag="IYE22_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ9506881.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="IYE22_001670"
     CDS             complement(5049..5579)
                     /locus_tag="IYE22_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ9506882.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IYE22_001671"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IYE22_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ9506883.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="IYE22_001672"
     CDS             complement(6436..6831)
                     /locus_tag="IYE22_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ9506884.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="IYE22_001673"
     CDS             complement(6828..8240)
                     /locus_tag="IYE22_001673"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ9506885.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IYE22_001674"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IYE22_001674"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ9506886.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9095..10555)
                     /locus_tag="IYE22_001675"
     CDS             complement(9095..10555)
                     /locus_tag="IYE22_001675"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ9506887.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMDWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="IYE22_001676"
     CDS             complement(10725..11882)
                     /locus_tag="IYE22_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ9506888.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IYE22_001677"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IYE22_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ9506889.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="IYE22_001678"
     CDS             complement(13349..13876)
                     /locus_tag="IYE22_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ9506890.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         3645 a         3034 c         2489 g         4718 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagtcag gaaacgccag aattgagcat tgatcttcgc caacaaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg atcggcatga aaagattttt cccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gcttttacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt aaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aatccattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatctag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagaaactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatta atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcct
//
LOCUS       DACIRY010000065        13817 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.066, whole genome shotgun sequence.
ACCESSION   DACIRY010000065 DACIRY010000000
VERSION     DACIRY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.066"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1206
                     /locus_tag="IYE22_001679"
     CDS             <1..1206
                     /locus_tag="IYE22_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ9506891.1"
                     /translation="GFDPAYRTGCKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPA
                     FIQLINKYQVDMVAIGNGTASRESELFVAEQLKSADHKTYYAIVNEAGASVYSASEIA
                     RKEFPHLQVEERSAVSIARRLQDPLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVV
                     ETAVNQVGVDVNTASPQLLQHISGLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPK
                     AYEQAIGFLRVPGGKNILDNTGIHPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLS
                     VEKLAESLSVGEETLADILAGLTQPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTV
                     RNVIDFGAFVDIGVKQDGLVHISKLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRIS
                     LTMLSPYEE"
     gene            1226..1708
                     /locus_tag="IYE22_001680"
     CDS             1226..1708
                     /locus_tag="IYE22_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ9506892.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            1724..2485
                     /locus_tag="IYE22_001681"
     CDS             1724..2485
                     /locus_tag="IYE22_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ9506893.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            2616..3950
                     /locus_tag="IYE22_001682"
     CDS             2616..3950
                     /locus_tag="IYE22_001682"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ9506894.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            4257..4742
                     /locus_tag="IYE22_001683"
     CDS             4257..4742
                     /locus_tag="IYE22_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ9506895.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            4735..5418
                     /locus_tag="IYE22_001684"
     CDS             4735..5418
                     /locus_tag="IYE22_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ9506896.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            5544..8189
                     /gene="polA"
                     /locus_tag="IYE22_001685"
     CDS             5544..8189
                     /gene="polA"
                     /locus_tag="IYE22_001685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ9506897.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            8234..9070
                     /gene="mutM"
                     /locus_tag="IYE22_001686"
     CDS             8234..9070
                     /gene="mutM"
                     /locus_tag="IYE22_001686"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ9506898.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            9034..9663
                     /locus_tag="IYE22_001687"
     CDS             9034..9663
                     /locus_tag="IYE22_001687"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ9506899.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            9715..10047
                     /locus_tag="IYE22_001688"
     CDS             9715..10047
                     /locus_tag="IYE22_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ9506900.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            10359..10850
                     /gene="nrdR"
                     /locus_tag="IYE22_001689"
     CDS             10359..10850
                     /gene="nrdR"
                     /locus_tag="IYE22_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ9506901.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            10873..12246
                     /locus_tag="IYE22_001690"
     CDS             10873..12246
                     /locus_tag="IYE22_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ9506902.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            12246..13172
                     /gene="dnaI"
                     /locus_tag="IYE22_001691"
     CDS             12246..13172
                     /gene="dnaI"
                     /locus_tag="IYE22_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ9506903.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            13301..13741
                     /locus_tag="IYE22_001692"
     CDS             13301..13741
                     /locus_tag="IYE22_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ9506904.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         4733 a         2431 c         3033 g         3620 t
ORIGIN      
        1 ggatttgatc cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa
       61 gttttagcaa tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct
      121 gctggaccag cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga
      181 aatggtacag ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac
      241 cataaaacct attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa
      301 attgcacgaa aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc
      361 cgtcgcctgc aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta
      421 ggccagtacc aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg
      481 gaaacagcgg ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag
      541 catatatcag gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac
      601 ggagaattca ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat
      661 gaacaagcaa tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt
      721 atccatcccg aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa
      781 aaagagcttg gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg
      841 gctgaaagcc tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa
      901 cctggacgag atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt
      961 atggaagact tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc
     1021 ggcgcatttg ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc
     1081 aaaaaattcg tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg
     1141 atagaacaag tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa
     1201 gagtaacaat gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga
     1261 gatatcatta aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt
     1321 acgtacaact ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct
     1381 atttgaagag tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta
     1441 tcatcttcat ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt
     1501 gaaaaaaaca ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga
     1561 gtattatcga tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg
     1621 caaatatcgt tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga
     1681 ctttaatgaa gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca
     1741 gaggaatcta aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa
     1801 ttttgaggaa ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc
     1861 tgataaagca gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg
     1921 ggtggggcat gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga
     1981 agatggcatg agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc
     2041 cgataaggct tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc
     2101 actggagatt cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc
     2161 gacactgcaa cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat
     2221 tttcgcgaat gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt
     2281 tacagcaaaa gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg
     2341 agaaatggaa cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat
     2401 tcctcacatt gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat
     2461 aaaaacggat ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa
     2521 tttggttgaa attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta
     2581 aaagaatgga cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc
     2641 attttgttgg tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag
     2701 gtctaaatgt ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa
     2761 aagcaaatat cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca
     2821 ttgcaggaaa tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt
     2881 tggaagtgat tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg
     2941 ctgtaacagg ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg
     3001 gtgtccgtcc aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg
     3061 aattcttttc atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact
     3121 atgcaatcat gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg
     3181 tatttacagc tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg
     3241 acgatgctta tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag
     3301 aaaacgatga tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg
     3361 tctatcatgg ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata
     3421 tcttaaatgc tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag
     3481 ttgcagaaga aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag
     3541 cagacatgac cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg
     3601 atggcgcacg ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt
     3661 ttacgcgtac gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag
     3721 tttacttatg cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag
     3781 accttggagc gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat
     3841 tgttagatta tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg
     3901 aacaagcgta tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact
     3961 gcagtgtcct tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt
     4021 tttgcaactt atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa
     4081 atcaaaaaca tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct
     4141 ttttcacgac ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt
     4201 tacgctactt ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga
     4261 acataggtaa gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt
     4321 tatccttaac agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg
     4381 atgcaaatcc tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa
     4441 tcgttccatc agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg
     4501 gaccaggatc acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg
     4561 aaacactgac ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga
     4621 tttctgtaga agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc
     4681 aaccgccaca agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat
     4741 aatccaagta catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta
     4801 ggtatgggac ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg
     4861 caattagcaa cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa
     4921 ctgattttag taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt
     4981 ggatttattg cctactctct tttgaatggc ataatattag cggttactct tgcatactac
     5041 gatattggaa cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct
     5101 gttgtcggag taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct
     5161 ttaatcggag tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt
     5221 gagctgttca tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac
     5281 caaagcatcc gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta
     5341 ttcatggcat tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc
     5401 tttggacgga acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt
     5461 ggtatggtcc cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa
     5521 ctttacagta aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca
     5581 gtgtggcttt tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa
     5641 acggtcttca tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa
     5701 aagagcaacc gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag
     5761 aaatgtatgc ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa
     5821 tgccatatat acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt
     5881 atgaagcaga tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg
     5941 tcgtcgtgct ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg
     6001 atatcactgt aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa
     6061 aatatgatgg gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct
     6121 ctgacaatat ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac
     6181 aatatggctc agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga
     6241 aagaaaatct gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca
     6301 atacttcagc gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg
     6361 ataaactcgt tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata
     6421 tcgtagaaga agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag
     6481 aggaaatgtt tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata
     6541 cagaggaaat cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc
     6601 tatctgtatt cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa
     6661 aagtatatga tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag
     6721 gaatcgattt tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag
     6781 atatcgaagg aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat
     6841 acggcaaagg ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag
     6901 cgcgaaaagt agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa
     6961 aaaaccaaga agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga
     7021 tggaaataag cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct
     7081 ctgctcgctt aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt
     7141 tgaattcacc aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca
     7201 aaaaaacaaa aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag
     7261 caccaatcgt agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg
     7321 tcgagggact gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga
     7381 cattgaccca aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc
     7441 gattagatga agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga
     7501 tttattcttc tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg
     7561 aacatttgaa agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg
     7621 tatttggaat cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag
     7681 tgaattttgg tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca
     7741 gccgaaaagc agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag
     7801 aatatatgga aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc
     7861 accgccgtcg atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg
     7921 aacgaacagc tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga
     7981 tgatcgaatt ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag
     8041 ttcatgatga acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg
     8101 tgaaggaagt tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt
     8161 ggggaaaaac gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag
     8221 gtgaaaagaa aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg
     8281 gttagtagta ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca
     8341 accagagaca cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg
     8401 tagagggaag tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat
     8461 ggaaggaaaa tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact
     8521 tttctttttt gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat
     8581 gacaatcgtc gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga
     8641 gcctttgcca gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa
     8701 agcaatcaag ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt
     8761 agatgaagca ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc
     8821 caaagaggta gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc
     8881 cggaggtacc acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt
     8941 agcactgcat gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt
     9001 gaaaacaaaa gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat
     9061 cgcaaagtag gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca
     9121 gccaaagtct ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa
     9181 actgttgagc ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta
     9241 ttgcagcctg atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag
     9301 gaaaaacggc aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat
     9361 caaatcgcct ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac
     9421 gaaggtcatt atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact
     9481 cagcttcagc gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt
     9541 agtagtcaac ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat
     9601 ggaacaaaag aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt
     9661 tgagtatctg tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga
     9721 tcattaaaaa atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg
     9781 ctcaataaag aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa
     9841 gaaaagcagt accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg
     9901 aagcggcaag atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg
     9961 ctcccagaaa gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac
    10021 tatacaggaa tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag
    10081 atgagaaggt gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg
    10141 taagttattt ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca
    10201 ggggacacaa ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta
    10261 ggacagtcgc gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac
    10321 atcctttata taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac
    10381 ataataattc acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc
    10441 gtcgcgaatg tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc
    10501 cgttattagt aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa
    10561 gaggactcat ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg
    10621 tcaaccatgt cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa
    10681 ttggtgaata tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg
    10741 caagtgttta tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg
    10801 tggataaagc aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa
    10861 ggaggcattt ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga
    10921 agaaaagcgg ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca
    10981 ctaatcggcg gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa
    11041 tctggcaaag gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc
    11101 ccgcaatttt atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt
    11161 aaagaagata gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct
    11221 aaagcatttt tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga
    11281 cgattccaga agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa
    11341 aaaatcacaa aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat
    11401 caagaaaaca tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt
    11461 gaagcagatt cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt
    11521 gcaagtcttg atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc
    11581 gatgaattga cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta
    11641 tctgaaaaag aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca
    11701 aaagaaacag ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca
    11761 aacgcagcgc ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa
    11821 gcaatcaagt cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat
    11881 cttgtttctc aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta
    11941 gtgatcaaga ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg
    12001 gcacaaaaga aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat
    12061 caagcaaaac aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt
    12121 cgacgggaaa agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc
    12181 gagaaaaaag cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat
    12241 atctagtgga agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag
    12301 aaaagtatgc tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa
    12361 gcgcacacca agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg
    12421 aattcgtgca agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct
    12481 atgagccgaa gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag
    12541 cattgttagc acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc
    12601 caaaagatat tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg
    12661 cgagcttaga agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata
    12721 aaggactgta tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag
    12781 cgagagaatt agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg
    12841 ttgagatgaa acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac
    12901 gttcacctat tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag
    12961 acgaagtatt cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct
    13021 catcgaattt tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg
    13081 aagagccgct aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg
    13141 aaatgactgg tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga
    13201 cctagtagta aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca
    13261 ccgcaacaag taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag
    13321 tatatctgta gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac
    13381 aggttctgtt gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga
    13441 gagacaaagc atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga
    13501 aacgcttcag ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac
    13561 gtcattcagc aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat
    13621 aaatgaattg aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac
    13681 gatgtctatc ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata
    13741 accaataaca ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt
    13801 tttaaggaga tatggga
//
LOCUS       DACIRY010000066        13723 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.067, whole genome shotgun sequence.
ACCESSION   DACIRY010000066 DACIRY010000000
VERSION     DACIRY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.067"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            54..506
                     /locus_tag="IYE22_001693"
     CDS             54..506
                     /locus_tag="IYE22_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506905.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="IYE22_001694"
     CDS             520..1209
                     /locus_tag="IYE22_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506906.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="IYE22_001695"
     CDS             1334..3685
                     /locus_tag="IYE22_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ9506907.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="IYE22_001696"
     CDS             3765..4160
                     /locus_tag="IYE22_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506908.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="IYE22_001697"
     CDS             4485..5102
                     /locus_tag="IYE22_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9506909.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="IYE22_001698"
     CDS             5143..5442
                     /locus_tag="IYE22_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506910.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="IYE22_001699"
     CDS             5476..5643
                     /locus_tag="IYE22_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9506911.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6293)
                     /locus_tag="IYE22_001700"
     CDS             complement(5688..6293)
                     /locus_tag="IYE22_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9506912.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6418..9405
                     /locus_tag="IYE22_001701"
     CDS             6418..9405
                     /locus_tag="IYE22_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ9506913.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            9554..9763
                     /locus_tag="IYE22_001702"
                     /pseudo
     CDS             9554..9763
                     /locus_tag="IYE22_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9898..10074
                     /locus_tag="IYE22_001703"
                     /pseudo
     CDS             9898..10074
                     /locus_tag="IYE22_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            10056..10412
                     /locus_tag="IYE22_001704"
     CDS             10056..10412
                     /locus_tag="IYE22_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9506914.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
     gene            complement(10926..11093)
                     /locus_tag="IYE22_001705"
     CDS             complement(10926..11093)
                     /locus_tag="IYE22_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9506915.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(11181..13121)
                     /gene="tet(S)"
                     /locus_tag="IYE22_001706"
     CDS             complement(11181..13121)
                     /gene="tet(S)"
                     /locus_tag="IYE22_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAQ9506916.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            complement(13122..13208)
                     /locus_tag="IYE22_001707"
                     /pseudo
     CDS             complement(13122..13208)
                     /locus_tag="IYE22_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
BASE COUNT         4621 a         2122 c         2496 g         4484 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttccatcat tgccaattgt
     6241 cgatccaaat tttgatcttg tgaagatact cgaatatagc caatttttcc cataaaaaac
     6301 tcctttttaa aagtgatata atttacatgt aaaatttact tgtagtttta ctgctatcat
     6361 agcattttta aaaataaatt ttatgtagac ttaaaattta cttcatggag ggtttaaatg
     6421 gatgaaatca aaagtaacaa aaaagcattt ggcatttatt taggagaacc tagtacagaa
     6481 caattaaacc aattttttta tttgcatgaa aaagatttag aattgatgga gactatacga
     6541 cgaccagcta ctagattagg ttttgcacta cagttagtct cactacgttt tttaggaact
     6601 tttatattag atcttaccaa tattccccaa aatgtattga gttatgtagc aactcaacta
     6661 aatattgatc ctggagaaat tatctactat agcaggagac agaccaggta tcagcacaca
     6721 aaattgataa aatcacatta tggatatcaa gatttcaacc aaccggaaat agaaacatac
     6781 ctagctaact ggcttttaaa tagagcgctg tatacaacag aatcttcaga tatgttgttt
     6841 gatatgcttc taaaaaaatg tctggatgaa aagattgttt tacctggaat tactactttt
     6901 tctcgttttg ttgctagtgc aattgaaaaa gctgaagagc atttataccg acaattgtct
     6961 ttggttccaa ctgaagatga gagaaagcgg cttctcaatc atttagaatt aattggaact
     7021 ccagtttatg gggcaataat caaaatggac attttgagaa ccccgttagc tgacgatagt
     7081 cataaagaaa tagccagagg ttttgaccga ctaaagcaat ttcaagcttt ctctactgaa
     7141 aaatggaaga tggaatctat tcctgaaggg aaaataaaag ttcttgcaaa ttatgcattt
     7201 aaagctaaag ctcaattaat tcaacgaatg agtgaacaga aaaaactggc gttgttagtg
     7261 gcatttattt tcatatacaa acgtaaggct atggatgaac aaattttagc actatctaat
     7321 ttttttgaaa ctgtttttag acgcgccaaa aataaggaat ctaaagaacg tcttcgtacc
     7381 ataaaagatc tagatcgagc cgcatcgaca ctttcagata ttgtagattt agtaatgtcc
     7441 agtcattctg atactaatga actatccctt cgcgatcgta tcttagaaaa atattctgag
     7501 gaaaatgtta ccgaagctgt atcgcaagtt cggctattgg ttaaaaatga tcgtgaacca
     7561 atagccattt ctgaacttct gaacacttat cgaaagttca gaaaatttat tccggatatt
     7621 ttaactacac taacatttga cagcaatgac tacggtaaca actgtaagga actctggacc
     7681 ttcattaata atagatttcc taagccgatt acttacaaaa tgtttggagc tattaaacat
     7741 actcttccaa agaaatggat ttattacatt catgaatacc ctgaacaaac taataaatgt
     7801 gtattgattg cagctgtcga attgttaatt caatctttaa aacgccatga tatttatatt
     7861 cctcagagtg tcttatataa caatcctatg gattgcttga ttgatgagaa aacttgggtt
     7921 caacaaaaat acgttttatt agaacaacta gatcttcctt cttcagcaaa tgaaatgtct
     7981 ttatggttaa agaacgattt ggaactgtct tataaagaag cgctaaatcg ttggcctact
     8041 tctaaaatgg ccagaataga aaatgcagat cgcctggttg tctctagact taaaaagaca
     8101 tatgaaaaaa aggaagataa gtcttttaga cgtcgcattc agcagttact tcctaaaatt
     8161 gatttgtcag atttgttatt ggaagtaaac caacagttaa atcttactca atcatttaac
     8221 cacttaagtg acaaggacac caaaatgaaa gacttggata ttagtttatt agcagttctt
     8281 ttatcagagt cctgcaatat tggtttttcc ccggtttcaa aagaaggatt tgattcttta
     8341 aagtatgatc gtcttgtcta tgtcaatcat cattatattc gagtggatac actttcagga
     8401 gctaatcaaa aaataataca tgcacacagg aaacttgcca cctctaagat atggggtgat
     8461 gggcatatgg cttcagctga cggtattcgt tatatcacac cacaacgttc cctatattct
     8521 agaagtaatc caaaatattt tggccgtggt cgtgggatca cattctacaa ttttgtctct
     8581 gatcaatata tagggttcca cggaatggtc gtttcaggga cactgcgaga ttcgctttac
     8641 cttctagaag gattcctcaa tcaacccagc gaattaactc ctaatcagat catgactgat
     8701 acagctggtt atagcgatat gatttttggt ctctttggct tattagggtt tcaatttagt
     8761 ccaagaattg ccaatgataa aggaaccaaa ctttggcgaa tcgacgctaa cgctcactac
     8821 ggggaacttg atacgttgtc tcaaaattca attaatatgg aactgattta tacccattgg
     8881 gaggacattc ttagggttgc aggttcgttg aaatctggaa aagtgaatgc aacagaatta
     8941 acgaaatctc ttcaacggaa tgggcaaccc acgcctttag gaaaagcaat aactgagtat
     9001 ggaaaagttt ataagacgaa acatcagctt cgttatattt ctgacgaaat atatgcacgg
     9061 caaattttag aacaattgaa taagggggaa gctcggcatg ccttatgccg taatattttt
     9121 tatggacgaa aaggaaaact ctaccagact tattatgatg ggatggaaga gcagctcaat
     9181 gcattaggat tagttactaa tgccatcata tattggaact cactgtattt agaaaaagta
     9241 tttgaacaaa tgaaagctga aggattcgac tgttccgaag aaatgatacg caggcaatct
     9301 ccactaatta gtgatcacat caattttgtt ggtaagtata cgtttagata cgaagctgaa
     9361 ttatccaacg gtaagttacg tccattgaat gtagaagaat tctagcaatg ttcgggtttt
     9421 gcatgctacc gcactatttc gtatgactgt tccagcgaat cacttcgaat agaaaaagag
     9481 aaaaacacga gaacagcgtt aaatcaacgt ttcttgtgtt tttacttttg tgctaccgca
     9541 ctatttcgtt tctatgttcc atgtacccct ttatcaagaa gcgcaaacac gccaaattgc
     9601 cgattggttg atcggcatga acgcaagccc tttatatacc ttgaatttac aacaaaaagg
     9661 cgtacaaggt acattttcat taggacgtgt acaaacaccg actttatatc tcatttatca
     9721 acgccaggaa gcaatagaaa actttaaaaa agaacctttt ttcctgaata attcatagtt
     9781 ttactaatag tctttcctaa cattgattta atagtcttta aagggttctg ttgcaaagtt
     9841 ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg
     9901 aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt gggctactat
     9961 cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg tggcattaac
    10021 gtttctcata cgacgattta tcgttgggtg caagaatatg gcaaactact ctatcaaatt
    10081 ttgatttcaa accattgggt cttgcgatta aagaagcccg tcttagtaaa ggattgactc
    10141 gtgagcaggt aggatcaatg atccagattg cgccacgcta tttgacgaac attgaaaata
    10201 aaggacaaca gccaagtttt catgttgtat atgatttagt gactttgcta aatatttctt
    10261 tggatggatt tattttaggg gaagaaaaca gtcataaaag ctccaaacgt cttcaagtag
    10321 aagcacagtt ggattgtctg gatgaagatg aattagtgac gattgggaaa gtgattcaag
    10381 caatttacgg agaaaaatgt aaatttaact agttcaataa tacacaatgg attaattaat
    10441 actatcagga aagattgatt acaaagatgt aatgaatttt ttctgatttt tgttaatcat
    10501 aaatagaaaa tgaatttatc tttgttacac ttaggaacta gagcgaatga taaatagttc
    10561 tttggttact cttatcgctg caaagtacag ttcatttttt gttgttggga aaattttctg
    10621 gatttattgc atatcacttc atccgactaa aaatttttga ttcccatagc gaagttcgtt
    10681 tttgttcctt aggaaaaact tgactgatat actagttttg aaatgacgag taactctgta
    10741 cttcatcaat aatcaagtat tgaattttaa ttgtatactt tttatgcttg agggatgtct
    10801 actgtaatac agctactgca aaaaaaatca gtacacttat tttcttataa aggatatcaa
    10861 gaactaagct caatggtatg catagaaaag aaaaagccga taaaatgaga ttttgatatc
    10921 tcgaattatc ggctctgcgt ctttgcgtct ggctctgtaa ttacagttat tctaaacttc
    10981 gttatttcaa ttaaaatttc ttgtctgcat ttaggacaaa atagagggaa attttttaat
    11041 tcagtatctt ccctcatttt taaacgggtc ttattgccac atatagggca cagtatccac
    11101 ctagtattta caatatctat atcctcctgt tctaattaac aactcaatat tcattttaaa
    11161 aatttctctt tagttcacta tcaatgtaag ttgattttat taaacatatg ccttactttg
    11221 tctattctat tatttggacg acgtggttgg aaagcagact taatgttagt aacctgataa
    11281 ccttttaact ctgttaaaca gacactgcgt ccatttgtaa agaaagttaa actgtttcga
    11341 tactcttgaa tacaacgggc tggaatttca ccaatgagaa tgacctcgtt accttttaac
    11401 ttagtattta aaatatttgc acaatatttg gaagcatcat tatatgctct cgaaagatat
    11461 tcttgtggta cataaatttc gaagctaaga tatggctcta ataactctgt accactcttt
    11521 ctaaaagcct gctctagtac aataggcgca agcattcgga aatctgctgg cgtactgaca
    11581 gggctgtaat atagaccata cttaaaacag atcttacagt ctgttaattt ccaaccgtac
    11641 aatccttgtt cacacccata gcgtatacct tccataactg cattttgaaa tgattgattt
    11701 aaataaccta gagaaaccag gctctcatac tgaatgccac tacccaaagg aagtggtgtt
    11761 acagataaac caatagaagc ccagaaagga tttggaggta cttcgatatc aatggtaaat
    11821 tcagattttt ttaatggtct ttccatataa atgacagttg gttttcttgt ttctatctca
    11881 atatgatatt tttcttgaat cagagtacaa gttacctcca tttggacctc acctaaaaaa
    11941 gatagcacaa tttcgtgagt tactgtatct acataatatt gtagaagggg atcactatcg
    12001 gatatttcaa aaagtgcatc tagtaacttt tctctttgta ctgatttaca tggttcaatt
    12061 gttgtttgta acataggaag aggattctca agaatttctc tatgtggtaa tcttttttta
    12121 tctccaagta cgttatttag ttttaaaagc tcatttttta aaataataat ctcaccaggc
    12181 tctgccttat ctatctggcg taattctcca tttattgaag tatacatttc tgtaactttt
    12241 attttttcct tttctgatat attgactgag tctcgcaaat gtagcgttcc actataaaga
    12301 cgtacataga caagccgttg accatcatct gaatattcta ctttaaaaac atttccacaa
    12361 agtttatctg aattgagttg tgtgggtgaa aataatttgc tagtaattac ctctataagt
    12421 tgtttaattc caatattatt ctttgcactt ccgtgataaa caggatataa ggagcaactt
    12481 tgaattcttt cgttttcctc cttttcaagt tctgcaatat tcaatgtttt cccaatggta
    12541 tatttttcta ataaataatc atttcccaca attactgtct cccattgttc tggttcagta
    12601 taatctatta cataaggttt caaatttaga ttcacagtct gcttaattat gatgtcgtca
    12661 gaaagtttgt ccttaatatc ttgataaaca tctggcaaat ttattccatt ttgatcaatt
    12721 ttgttaataa aaaatattat aggtatgttc atctttctaa gtgcatggaa tagtatacga
    12781 gtttgtgact gtactccatc ttttgcagag attagcaaaa tagctccatc caaaacagat
    12841 aatgaacggt atacatctgc caaaaaatcc atgtgtccag gagtatctac aatatttact
    12901 ttaacatttt cccgttgaaa agatgttatt gcggtctgaa tagtaatacc tctctggcgt
    12961 tccaaaaaca tagtatccgt tttcgttgta ccgctatcta cacttcctaa ctctttaatt
    13021 gctccgctac tgtatagtaa gctttctgtc aaagtagttt ttcctgcatc aacatgtgct
    13081 aagataccga tattaataat tttcaatttt atttcctcca ttcaaaagct caaaaagcag
    13141 aaaaaatccc agtgataaat actcttatca ctgggatttt aatacaaaaa tagctgtata
    13201 ctacacactg atagccacag atgctttatg ataaaatgat aaaagtattc ttaaactggg
    13261 cacaaaaaac cagtccttat tttaaataag gatgcttaat taattattcc cgctatctaa
    13321 ttgacagttt atttaagaat accttgccgc atttttatta tctccttttg aatgttatct
    13381 taattctatc atatgataga atgcttgtaa agccaaccat attttttgaa cgaaagggaa
    13441 attaatgaga aattaatgga agttagtgta gtagttgaag ataactcttc agaatggcta
    13501 tcaaaattca gcgatttaat aacgatttac aagttcataa gggattcttt attcatccag
    13561 ctattatatt acttttcagg tcaataggac aactgtccac aacctttcta caaatatatt
    13621 acgtataatt tttgaaatat atactgctta acaaaatatt ttgcagtggt tccaatagtt
    13681 ttgaattgtt aatattaagt ttgacttgga aaatatcatt aaa
//
LOCUS       DACIRY010000067        13064 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.068, whole genome shotgun sequence.
ACCESSION   DACIRY010000067 DACIRY010000000
VERSION     DACIRY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.068"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..607)
                     /locus_tag="IYE22_001708"
     CDS             complement(<1..607)
                     /locus_tag="IYE22_001708"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ9506917.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDT"
     gene            complement(656..1291)
                     /locus_tag="IYE22_001709"
     CDS             complement(656..1291)
                     /locus_tag="IYE22_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9506918.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(1357..2760)
                     /locus_tag="IYE22_001710"
     CDS             complement(1357..2760)
                     /locus_tag="IYE22_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ9506919.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(2747..3778)
                     /locus_tag="IYE22_001711"
     CDS             complement(2747..3778)
                     /locus_tag="IYE22_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506920.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            3809..4030
                     /locus_tag="IYE22_001712"
     CDS             3809..4030
                     /locus_tag="IYE22_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ9506921.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(4195..4932)
                     /locus_tag="IYE22_001713"
     CDS             complement(4195..4932)
                     /locus_tag="IYE22_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ9506922.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(5085..6425)
                     /locus_tag="IYE22_001714"
     CDS             complement(5085..6425)
                     /locus_tag="IYE22_001714"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ9506923.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(6662..8185)
                     /locus_tag="IYE22_001715"
     CDS             complement(6662..8185)
                     /locus_tag="IYE22_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ9506924.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(8564..9175)
                     /locus_tag="IYE22_001716"
     CDS             complement(8564..9175)
                     /locus_tag="IYE22_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9506925.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(9298..9423)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(9505..10221)
                     /locus_tag="IYE22_001717"
     CDS             complement(9505..10221)
                     /locus_tag="IYE22_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ9506926.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(10227..10796)
                     /gene="scpB"
                     /locus_tag="IYE22_001718"
     CDS             complement(10227..10796)
                     /gene="scpB"
                     /locus_tag="IYE22_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ9506927.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(10780..11577)
                     /locus_tag="IYE22_001719"
     CDS             complement(10780..11577)
                     /locus_tag="IYE22_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ9506928.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(11547..11918)
                     /locus_tag="IYE22_001720"
     CDS             complement(11547..11918)
                     /locus_tag="IYE22_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ9506929.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(11937..12824)
                     /gene="xerD"
                     /locus_tag="IYE22_001721"
     CDS             complement(11937..12824)
                     /gene="xerD"
                     /locus_tag="IYE22_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ9506930.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(12920..>13064)
                     /locus_tag="IYE22_001722"
     CDS             complement(12920..>13064)
                     /locus_tag="IYE22_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ9506931.1"
                     /translation="EEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRN
                     QSV"
BASE COUNT         3674 a         2812 c         2232 g         4346 t
ORIGIN      
        1 tcgtatcgat acgaacagca tcctctgctt gcttcagcgg agagacgtcc cttgttgaat
       61 cataatggtc tctttcctgt atctctttcg tcagtgtttc aagatcagtc ggaattcctt
      121 tttcttggtt ctctttgaaa cgtctttctg cacgctcact tacgcttgct actaaaaaga
      181 ttttcacttc tgcattcggt aatacagctg tcccgatgtc tcttccgtcc atgacaacgc
      241 caccgcgttc ccctattttt cgttggacag caactaattc ttctctgact tttccatgtg
      301 cagagacttc agaaacagct tttgtgacat ctggttgacg gatttctaaa gtcacatctt
      361 ttgatgctat gaatacatgc tgtccatctt cttgttgcga aaaagagata ggatactttt
      421 tgatcagcga aactagccct tcttcatctg aaaaagatac attgttcgta atagctaaat
      481 aagttactgc gcgatacatg gcaccggtat ctgtataaat ataattcaat tcttttgcta
      541 atatcttggc aacggtactt ttcccagatg aagcaggccc gtcgattgca atactgattt
      601 gtgtcatgat ataactcctt tttctcagaa aaaaagccgc aaagcgcggc tattattatt
      661 taattcttaa ttcttgacca gggtaaagca taaagttgtt tggatctaag ccattcaatt
      721 caaacaattg atcagtggtg atccctgcac gctcagccac ttgttttgga ccttcgcctg
      781 cttgaacagt cgttgttgaa ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat
      841 tgctgcttga atcccctgct tgataagttg cctcacttga ggaagtcggt gttgtctctg
      901 ctgcaatact gctttctgac gttacacctg tatcttgaga cacagtagat tcttccgcga
      961 ttgtactaga ttctactgtt gaagtctcag ttgactcctc ttttgttgta cttgtagaac
     1021 tttctactac tgatgtcgaa gtttcagaag aagttgctgc tgttgcattg ttgcttgatc
     1081 catttctgat ccagaaataa gcacctgctg gaatagcgat acataagacc agtaaaatca
     1141 aaaatagcga aagaaataat gtatttcctt tcttttgttg tctctgagaa cttcttgatg
     1201 aaacttcatc atctgtatcg tagattggtt gttcccatgg ttcttgtgtt tcgttatttt
     1261 ctgtagattg atgtctatct tttctgctca ctgttttacc tcctaaactc tatcgtgtgt
     1321 attataccat aggaaaaagt gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa
     1381 taaatttagt aaaatcgttt cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt
     1441 tggcagaagt tctgcttttg ctccatcaat atcacaacaa ttctgcggct tttcggagag
     1501 tttttcgcca aaataagcta agatgaattt tctgcggcag ttttcttcat taatatagcg
     1561 aagcatctgc tgcaggcgga attctttttc tttttcctga cgtttgagcc gttccaccca
     1621 attttctggt ttttcagact gctggatcag ctccatccat tttttttgca gctcatcaaa
     1681 gggagcttgc tttgtggcat attccaaata aagctcaaaa ctttgtcgct gttctctaga
     1741 aagttgatta aagaaataat ggatacgttc atccccactt tgataaaaaa gaattgctgc
     1801 actttcttcc tgatctctgc ctgctcgacc gatttcttgt acatagtttt ctagactgtc
     1861 tggcaaatca taatggacca cataacggat atcagattta tcgatcccca taccaaatgc
     1921 attcgtagca atgagaaact gcagctgatt ttttacaaac tgctgttgaa gttgtcttct
     1981 ttgagctgca tcaagaccac cgtgatagta tccaactgta aaacgttccc tgaataaatg
     2041 atacaatcgt tcgacttctt tttttgttgc acaataaatg attgccgctc cttgtgctct
     2101 ttccatgaag tgttcaagtt cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat
     2161 attttgccga ttgactgatt gtctgatctc ttttggtggt cgatcaaata gtacatgacg
     2221 tatatctctt gctactaaat ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc
     2281 taaaagcttt cggatttttc ctaactgttg atattctgga cggaaatcaa ctccccattg
     2341 agacacgcaa tgagcctcat ctactacata taacgcaata ttttgttttt tcagatgctc
     2401 taaaagagaa ggattagtca acatttcggg actcaaaaac aaaaatttat attgcgatag
     2461 atgacggagg acgtatcttc tttcagattg cgaaagtaaa ctattgaaag cagctactcg
     2521 tttctcccct cgtttttgta attgcgtcac ttgatcttcc attagagaaa gcagcggtga
     2581 cacgatgatg accagacctt ccattaaata ccccgtcaat tgatagcaaa ggctttttcc
     2641 cgttcctgta ggcaaaatag cgagagtatc ttctccatca agcaacgcct gaataatttc
     2701 ttcttggccg ggacgaaatg aatcaaatcc aaaatatttt tttaattcat ttattaacat
     2761 tctgtatcgc ttcctttctc aatagataga tttgatacaa acgaaattct ccataatcta
     2821 attgtccatc tacattccat tcgctaaagc gccactctcg cacggaatcc ttatttggtt
     2881 ccaaacgttc gattgtttct gcagagagaa tccactcgta tggaaaatca tcaaaaaaca
     2941 aggcccactc aataagatga tctgttactg ttcctctttt taaatgacgg attgccatga
     3001 tctggtcaat tgtctgacca ttcataaaca tctgcttggt gatcatcatg ctttgattga
     3061 aattttgctg taataatgaa tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga
     3121 tttgtgctaa caaaaggtgg atacatctgc tttgttgcag ataaacctct gactcatcat
     3181 tgttttttgc cagctgataa ggaagcagcc ctgttcgctc atttccagat aattggttag
     3241 ctaaaaaatc tgctgcttct tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg
     3301 cagtatcaat taaaatttca cgtggcaatt ggctttcact aagccatttt tttagttgga
     3361 aagtataaaa aggacttgtc tctgctggaa gatagtcttg aatacccgtt gccagattag
     3421 aaattacttg aacagcaaat ttaatgagcc gccaactagt ttcccctgtt ctgccatagc
     3481 ggtcaaaacg caaaccagta tgttctattt gatgttcgga caacatatca gcccctgcag
     3541 gcgttagctt tgcttcattt tcatttattt cgatccaacc ttgctgcgca attttttgca
     3601 aaatttgata aaacttatcc tgcttcaatt ctggaaaact gttatggata aacaaaagtt
     3661 cgtgggtaaa tccataaata agtactgaag tcgttcgttt tcccacaagg agttggtaaa
     3721 gagaactaat ccgcagcttg tgtcctgatg aaaatagcga taaaataaaa acaatcatat
     3781 tattctcctt aagttaggat ggttagaaat gtcactacta tgtaaacttg tacctgaacg
     3841 ctgtattgca tgcggtcttt gtcaaatcga agcgccagat attttcgatt atgatgaaga
     3901 aggaatcgtt ttatttgccc aagaaccaga ggctcatcaa cagttcgtga tgccggaaga
     3961 agaaacatat gtaaaaaaag cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa
     4021 taaaatataa atataaacga aactttaaaa acgtacacta agttctctaa acttgaaaat
     4081 aagccgggat ctgtttctct tgttccgtga aatcccgact tattttgaaa gattcatttg
     4141 ctatctatag ttaattgact agttttttca cggaccctat aggacccatt tatattattt
     4201 caactttttc agccagtttt ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga
     4261 aatttctgcc tctccatctc ctttttgctc accatagctg ccaaaacctc catgatttcc
     4321 gccattgatt gatacatact ctgttctttc cggtaaatac tgcttagctt cttgataagc
     4381 ttcctgattc aatactttat cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc
     4441 tgtcaatgtt ccttttttgt cgggataact tgccatgaag aatacccctt ttaaatgttt
     4501 gtctgaatga ttcttattga cataacgact tgccattacg ccacctaatg aatggccacc
     4561 gatcacataa tcggaatttg gaaaatcttc tttgacctta tccgctttgt ttccgcccat
     4621 cactgctaga tcaagtggca ttttgacaat agcgactgaa aagccgtttt ttgctaaatc
     4681 ttgcgcccaa atactataac tttctggctc tacgagagca ccaggataaa aaataagtaa
     4741 tggagacttt ccatgattct ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt
     4801 tccttctttt accaacactt gcgcttctga cgatggttcg tacgtatttt gctgtatata
     4861 gtaataaccg gctccaccga gtaatcctaa aatgatgact aagccaatta gtattcgtct
     4921 aaatattttc actttctccc accttttcta tctgagtgac ccctttttct taatcaagta
     4981 tgattatacc ttaaaaaaat aagagagaaa tgcaaacttt ccagcatcaa ataaaaaaag
     5041 acggaatatc catttgaata tccgcccttt ttgtatgttt atttttattt agcacataaa
     5101 cgatacacag cttctgcata aatttccatt gctttataca tactacttaa ttcccatcgt
     5161 tcattcgcct ggtgcataaa gtcaggagta tcagggaaca tcgcaccgaa tgccacacac
     5221 tggttcatcg tacgagcaaa agtagcgccg cctgatacaa acggttcagt catgtctcca
     5281 gtaatatcgc gatatgtccc caatagattt ttcactaact cactgtctag tggcacgtat
     5341 aatgaatcaa ggaagtcaaa ttcttcataa gtcaacccat atttttctgc tgttttagtt
     5401 aatttagcta ctaaatcatc tttcttgaat gtaacaggga ttctcatatc tacaccgatt
     5461 tttgtttgtt ctggtgtgat ttccaaactt gcaaagttca tcgttagctc gccggattgt
     5521 tcatcttctg ttttgcccac gacagcttca ccagttgcat tttcttgaac taattttcct
     5581 aagaaattga ttgggccaaa atcaaatact tctgaaagag caattgccag acgagaaata
     5641 gcattcactc cttgtgctgc atctttcgcg tggatacttt taccaagaac gacgatgcct
     5701 tcaccttgtt cttcgaaatc gaattcgtgt tttttcaatg cttcttttac ttcagaaagc
     5761 ttttctccag agtaaggagc tgcatcagga acaacattca acgctcctcc agcttttaca
     5821 gaaaactcgt tcgttcctgg tcctgtcaag taagcttgca ataagccttt ttctgcataa
     5881 attaaaggaa attcagcatc tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta
     5941 tatttttcta aacaacgcca taaattttct tcatctgtac caaagataaa acgaatacgt
     6001 gtcttgaatt ccacgccagc atcaatcaaa gctttgacgg catacattgc cgcaatagaa
     6061 ggacctttgt catcttggga tccgcgacca actaaccagc cgtcttttat agttggatcg
     6121 aatggttttg tttcccaatt attttcgtcc cctgctggaa caacgtccat atgacacaag
     6181 ataccgaata attcatctcc agagccaatc tctgaataac cgtaatagcc ttctggatct
     6241 ttaaatgttc taaatcctag cttttcactg atttccaata ctttatccaa agcttcaatc
     6301 acttttttac cgaagggatg tccttgtcct gaatctgctt catctagtac ggaaggaatt
     6361 cggatcaatt cgctcaatga ttctaatgct tgttcatgat gttgttctgt cacaaatttt
     6421 ttcatgatat tatcctccta atatgttaaa aagggtcatg tcaaattcct ccgacacaac
     6481 ctcttcttct caaataatga cgttttaagc ctgctccatc agcaagcaga atgttcttaa
     6541 caaatgatga aagaaaaaac actcactgaa tggagctatt gacttatcca cgtgcttatc
     6601 gatagccgtt tatcggtttc ttcattctta tgatagacct tgtacctgta tttgtctagg
     6661 tttagaacat tgctgctaca cctaggatca cacagatggc tacaaataat atcgcaatta
     6721 atttaccggt aaatttccac cacactgaaa catcgaaacg agcaattgct acagcaccca
     6781 ttacgatagc agatgttggt gtaattaagt ttactaaacc agatgcagat tgataagcag
     6841 tgattactaa atccttaccg acgccggcaa attcacccat aggtcccatg attcccattg
     6901 ttgcagcagc taagccggaa gttgaaggaa tcaagaatga catagggata tagaaaataa
     6961 atgtaataat gatgaataca cttgaagata atgaacttaa tccttgttcg ccccaatgta
     7021 agatggtgtc tgtgattaat ccattgttca taaccacttg gataccacga gcaacagcta
     7081 ctacgattgc tacaccgatc aaatcagaag caccagctaa aaattcagag acaaattcag
     7141 actctttcat gccataagtg attgctacta cgatggacat tactaagaat aacatagtaa
     7201 tttctgggaa gtaccagtca cctaatggaa gcatgctttt acctagaacc gtacctagta
     7261 ccggaaggtt tgttaaccag tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg
     7321 ttgtccaagg aatcaaactg atgatcatga taccaaaagt aataaagaac aatacattaa
     7381 catgtttttg ttttttcgtg attacttctt gacgaccgat tgcttcaata tcgaaatgct
     7441 tcatatcttc tttacgttgg ttgtatacta atgattttgt tggatctttt tcgatctttg
     7501 atgcataacg atacacatat acaatagaaa tagctaacaa aataacgaac atcaagaatc
     7561 gaagaccaat accttcaccc atcgaaatcc caacagcttg tgaagctacg cctgttgcaa
     7621 aaggatttac cgttgaagct agacagccta cttgagaccc tactaatacg attgctacag
     7681 ctactactgt atcaaagcct acagccatca tgactgggat cagtaaagca tagaatggaa
     7741 tcgtttcttc tgccattccg taagtagagc cgcctaatgc aaataacagc atcaatactg
     7801 gaataagcat tttttctttt cctttgaatt tcttcacaac tgaagcaatc ccggcatcta
     7861 gtgcccctgt tttgttgata atacctaaaa atccaccaac aaccaaaatg aagaaagaga
     7921 ttgggattgc agcaggtgtt tctttagtta gctcccccgc tggtacacct agcatcccat
     7981 tgattggcgc catgaaaata tcccacagcc cttgaggatt ttgttgtact cgttcatatg
     8041 atccagcgac gatgttgcct gcatcgtcta cactatagtg cccagctggt ataaaccaag
     8101 taagtacagc gattactgca ataataataa ataagaccgt atacgctgaa ggcatttgaa
     8161 attttttctt ttttgtttgt tccatcttct tcttcctttc ttttccgtta gagacaacaa
     8221 ttttggctct aatgttattt tatcaaaaag agtatcagaa caaaaataat aacacgcatt
     8281 ttgtaaaact tttgttcaaa aagttcgttt ttccctattc cctcctcttt tttcaggttt
     8341 ttaaagcttt cttctttttc attgtagcgt ttcctttttt ttataaacaa acattttcgt
     8401 cttttaaaag gagaagaatt tcacatttgc aaagaattga ttgattttta tgcataaatc
     8461 aaaaaaatac tcaaaacaaa caaataaaac gtttacatta ttgcatgtct tgtatttttt
     8521 tccaaaaaaa aaagaccaag aaatttcttg atctttttga tatttatttg actgctgtat
     8581 gacgttgttc gaactgatgt tgttttcgac ttaaccatgg aagaagttgc gcatgcagca
     8641 ctaaaaagac agcactcacg atgaatcctt tgatcaagtt gaaaggaacg atcccaatca
     8701 gtacatagtt tgctaaagac atgcccagca tctgtgttgc gttcagccct aaaaacatta
     8761 agtaaagagg cgtaataacg aaataatttg cgatactcat aaaaatagtt agagcgatgg
     8821 ttcctgtaat gacacctaaa aatttgtttc ttcctttatg ctgtttcttt acactaaaga
     8881 aataataaat cgggaatgta aagatcgttg ttgataagaa actagcagtg tctcctacca
     8941 aattatccgg agaaaaacct gtagtcagca gatgtaatac tgaacgcaag aacgctgttg
     9001 caactccggc cgctggaccg aataagaaca tgctgatcaa taccgagata tcactaaaat
     9061 cgatcttcaa aaaactaaaa gccggcatga tcggaaaagc aaaatactgt aaaactaatg
     9121 cgatcgcagc aaacatcgct actgcaacca ttttctgtac acgggtgttc ttcatttgaa
     9181 atcctcctgt taggactcct cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa
     9241 ctctattttc cagggaaaat agagttagag ttatttgatc aagcgtcgtc aaataagctc
     9301 tatcttcttt atccagacta tactgtcggt atcggaattt caccgattca gctatttttt
     9361 tgcaaaatag gtcgtggact ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg
     9421 aaccttatat aatttttgtt tacatattca ttatacatat ctattcaaaa aacgcaacat
     9481 ctaacactta taaaaagtaa gtgttcattt tgagtggttc agcaaactgc tgacttcttt
     9541 tttgttcagt tcacggtaat ccccaggacg taggccttgt aaagtcagat ctccgtattt
     9601 ttcacgtttt agtttttgca caggaagacc aactgcttga agcatatttt tcacttgatg
     9661 attacgtcct tcgtggatcg tcaattccac gatactgtgg tttgtcttag gatccactga
     9721 taaaatttgg aatttagctg gagatgtttt tcgtccttcg atctttattc cttttgtcaa
     9781 aggtgcaagc atcgattttg tagcgatccc ttttaccttt gcaacgtaaa ctttatccac
     9841 ttcatgtttt ggatgtgtca ggcgctgagc aaaatctcca tcatttgtca gtagcaagat
     9901 ccccgaagta tcgtaatcca gtcgacctac aggatagata cgttctttta ccatcggcaa
     9961 aagatccgta acaacttttc ttcctttgtc atctgcaaca gcagaaataa ctcctttagg
    10021 tttgtacaat aaatagtaac cgtattcttc ttgataaatt gggacttcat ccacttccac
    10081 agtgtcatgt ttcccaactt ttatccctaa ttcccttaca acttgtccgt ttacttttac
    10141 acgtcctgca aggatgaatt cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat
    10201 tactttctgc aatctctcca ttacaattat tctcctttat tcattccatc aaagaataga
    10261 tctggtgaca cgtcttcttc aagtgaaatt tccatttctt gtgtatcagg cagttcttct
    10321 aatgatttca aaccaaaata atctaagaag tagccagttg ttccatataa tatcggccga
    10381 cctggtcctt ctactctacc tttttcttca atcagtttat gggcagctag acgctgtacc
    10441 gctcctgaaa cctgaactcc tcgaatctgc tcgatttcag ctcttgtgat tggctgttta
    10501 taagcgatga ttgctaaggt ttcgacggcg gcttgagata atgtgtttga tgtcccttgc
    10561 gcatatcgct taagtaaggg agccagctct tttttagtcg ttaatacata atggctttcc
    10621 gtttcaaaaa tatgcaaagc tgagtgcgag ttttgagtgt aagattcttt taattctttc
    10681 actaaacttg tgacttctgc ttcgctgaga tttaacagtc cgcttaattc ttcattactg
    10741 atcccttcct cgccaacaat aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc
    10801 ggtatctcct actgctggat aaagcataat tgattcatag ttttcttttt gttgcgcaat
    10861 gatctgccct tttttcatca attctagcaa agccataaaa gtagtgacaa cttcactttt
    10921 agaaggtgac tcaaaaaaag aatcgaaagt acagccatgc gcatttcttt ttatacgctc
    10981 ttcgataaat accattcgtt cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac
    11041 tttttgagaa aatttcttac gttctaagat tttctgcatg gcacggatca aatcgatttt
    11101 tttgacttgt cctcgcttca acggcttttc agcatccgct aacccatcga tgttcatagg
    11161 ttcttttgtg taataaagac ttcgttcctc tgctttttct gtcagtcttt cagctgcata
    11221 tttatattta cggtattcta aaagctgagt gaccaattct tctcgaggat cttcttccca
    11281 taattcttca tcgtctgtga cgaattcttg gacaggaagc aatgtcttgc ttttgattgc
    11341 catcaatgta gcagccatca ctaagtattc tcctgcaaca gctagctcga gtgttttcat
    11401 tgtatggata taattcatat attgctccgt gatcgtagcg atcggtatat catatatatc
    11461 gatttccatt tgctgaatca gatgaagcag cagatcgaga ggaccctcga atacatctaa
    11521 tttaatattg atctccgtca actcattcac tctttcttct ttttctttgc tagccatttt
    11581 ccaatgtaag gctctgtgat ttcagttgca cggatggaga catccgggta cttttcctgt
    11641 aattcatcta acatttgaaa gcccatattc tcaccgcgat aagacggatt caaagaaatg
    11701 tcatggatga tcagctgatc gttcgttgcc cagtaaatac caataatccc ttgaatgttg
    11761 gttgaacctt ctggcgtaaa gaaaaaaagt tcataatcgt cttcagattc gtacgtatca
    11821 atctccttca acaaagaagt tttacctgtc agtttcttat ggaaactcaa tagtcccata
    11881 gcaattttac ggttttcttt ccggtaatgt gttaacatct tctcaccttc ctatgactaa
    11941 gcccttggaa aatgctgctt ataaacatcc gtcattcttt tcttcgtgat atgtgtataa
    12001 atctgagtcg tagagatatc cgcgtgacct aacaattcct gtactgtccg caaatcagct
    12061 ccattttcta gcagatgtgt ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg
    12121 taaatcccag cctctcgtac taactgcttc agatttttcc agattccttg ccgagaaaga
    12181 cctttcccat gattattgac aaatacatgc atttcagatg cgtcctttac aagtaatgga
    12241 cgtgcttcgt ctaagtagcg ttccagccat tggatcgcat aatcacctaa aggaatgatc
    12301 cgctctttat ctccttttcc aactgtctgt accagtccaa gagaaagatg aagatctccc
    12361 aatttcaatc caatcaattc actgacgcgc atcccagtag catacatgac ttccaagatt
    12421 gcacgatctc ttatccctaa cgtttttgtc gtatcaggtg cttcaatcag cttttctact
    12481 tctgtcagag tcaatgtact tggtaatttc tgtgctttct tcggcgtatc gatatgttgc
    12541 atcggatcat gatcagtgat acgctcttgc cgtaaaaact gatggaatct tctgagactt
    12601 gagatcatcc gagtaacagt tgctgtcgct tttttttctt catgcaactt ctccagaaaa
    12661 cggatgacta aaaagcgatc gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa
    12721 tgcaagtact gttccaaatc acgttcatag ctttgtcgtg tattggcgga caatccccgt
    12781 tcaatattca agtagtggag ataatcgttg atttgctctt tcataataat ccccctcatg
    12841 cctctctatc atatcagata ccgcttcatt ctgatagtct ttttccataa tctgagcaaa
    12901 gaaaaagccg tctagacact cagacggact ggtttctttt ttctttttca tagcattcct
    12961 gacagattcc atggaacgtt agccgatgat cttttactaa aaaatgataa cgttcttcta
    13021 ctacttgctc cacttcaagt aaaagatcct cttctacttc ctcg
//
LOCUS       DACIRY010000068        13049 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.069, whole genome shotgun sequence.
ACCESSION   DACIRY010000068 DACIRY010000000
VERSION     DACIRY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.069"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IYE22_001723"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IYE22_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ9506932.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="IYE22_001724"
     CDS             1873..4539
                     /locus_tag="IYE22_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ9506933.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="IYE22_001725"
     CDS             4903..5655
                     /locus_tag="IYE22_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9506934.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="IYE22_001726"
     CDS             complement(5677..6831)
                     /locus_tag="IYE22_001726"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ9506935.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="IYE22_001727"
     CDS             7016..9457
                     /locus_tag="IYE22_001727"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ9506936.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="IYE22_001728"
     CDS             complement(9787..10491)
                     /locus_tag="IYE22_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506937.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10678..10923)
                     /locus_tag="IYE22_001729"
     CDS             complement(10678..10923)
                     /locus_tag="IYE22_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506938.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10940..11791)
                     /locus_tag="IYE22_001730"
     CDS             complement(10940..11791)
                     /locus_tag="IYE22_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ9506939.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11957..12961
                     /locus_tag="IYE22_001731"
     CDS             11957..12961
                     /locus_tag="IYE22_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ9506940.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4169 a         2315 c         2716 g         3849 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc attctttcta
    10681 ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt catgttcgtg
    10741 ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga tgatttgatc
    10801 atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca aaaagacaaa
    10861 atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt cgattggatc
    10921 cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct ttgccgacta
    10981 caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa tcaaataatt
    11041 cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg aattcgtgtt
    11101 gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt
    11161 tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct tcgaggatca
    11221 tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa
    11281 cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga tggtggggta
    11341 agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct tcaaatgctc
    11401 gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg tttggccagt
    11461 ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct tcaaaagaag
    11521 acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag aatatctctt
    11581 ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt
    11641 gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag gaaacagcga
    11701 catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg taaccatttg
    11761 atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta ttcgttcaac
    11821 tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc ttgaaatttt
    11881 tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt aaactaagac
    11941 agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata ttattgacag
    12001 agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag aatatattcg
    12061 tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa ctggacaact
    12121 agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt ttgtggaagc
    12181 aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag aagcacctag
    12241 tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat ctttattgaa
    12301 agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag ggatcgacaa
    12361 ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag atgaagcatt
    12421 cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg agttttctgg
    12481 caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac cttctttaga
    12541 agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga
    12601 agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag aagaaaaagg
    12661 tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat
    12721 ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc ctgtaaaaga
    12781 agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat
    12841 cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat ttgtgaataa
    12901 tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga atcgtttata
    12961 aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag
    13021 agaaggatcg gaaaagacga atataacaa
//
LOCUS       DACIRY010000069        13017 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.070, whole genome shotgun sequence.
ACCESSION   DACIRY010000069 DACIRY010000000
VERSION     DACIRY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.070"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            400..969
                     /locus_tag="IYE22_001732"
     CDS             400..969
                     /locus_tag="IYE22_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ9506941.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            956..1816
                     /locus_tag="IYE22_001733"
     CDS             956..1816
                     /locus_tag="IYE22_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9506942.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2006..2710
                     /locus_tag="IYE22_001734"
     CDS             2006..2710
                     /locus_tag="IYE22_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9506943.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2715..4550
                     /gene="walK"
                     /locus_tag="IYE22_001735"
     CDS             2715..4550
                     /gene="walK"
                     /locus_tag="IYE22_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ9506944.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4547..5863
                     /locus_tag="IYE22_001736"
     CDS             4547..5863
                     /locus_tag="IYE22_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506945.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5864..6733
                     /locus_tag="IYE22_001737"
     CDS             5864..6733
                     /locus_tag="IYE22_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506946.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6788..7597
                     /locus_tag="IYE22_001738"
     CDS             6788..7597
                     /locus_tag="IYE22_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ9506947.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7700..8329)
                     /locus_tag="IYE22_001739"
     CDS             complement(7700..8329)
                     /locus_tag="IYE22_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ9506948.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8499..9791
                     /locus_tag="IYE22_001740"
     CDS             8499..9791
                     /locus_tag="IYE22_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9506949.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9867..10529)
                     /locus_tag="IYE22_001741"
     CDS             complement(9867..10529)
                     /locus_tag="IYE22_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ9506950.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10754..11038
                     /locus_tag="IYE22_001742"
     CDS             10754..11038
                     /locus_tag="IYE22_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ9506951.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11089..12714
                     /gene="groL"
                     /locus_tag="IYE22_001743"
     CDS             11089..12714
                     /gene="groL"
                     /locus_tag="IYE22_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ9506952.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4336 a         2232 c         2801 g         3648 t
ORIGIN      
        1 ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta agaattttaa
       61 aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata ggaaatcatt
      121 catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa agttcttgca
      181 agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc tccccggcgg
      241 gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt ttgttttcat
      301 ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt atcataagtc
      361 tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat gcgtcagcaa
      421 ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga agaactccct
      481 gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct ttcacctgtg
      541 atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta cgttaaacta
      601 ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc atttttactt
      661 gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg gatattgttc
      721 caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga acaagaagca
      781 gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa attcgaccag
      841 gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc aatgaaactt
      901 cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa aacaaatgaa
      961 aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag aattgataaa
     1021 aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg tatatacaga
     1081 taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa tccctggtac
     1141 atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa aagacggtta
     1201 tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata atgtggttcg
     1261 tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga aaaatattgg
     1321 tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag gagtagaatg
     1381 ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt tctttaatgt
     1441 tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt ctattgtagg
     1501 aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt attacacagt
     1561 aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg tcaaagaaaa
     1621 aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga aagaagaagc
     1681 aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa tctactttgg
     1741 ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg tcggtgtaca
     1801 agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt gttttccttt
     1861 tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt aattttctct
     1921 tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag aaggtatgac
     1981 aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg atgagaagcc
     2041 gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt atactgcata
     2101 tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga ttttacttga
     2161 tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca aaacttatga
     2221 tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc taggcttaga
     2281 gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag ttgcacgtgt
     2341 caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg cagcacctgc
     2401 ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga cgaaacgagg
     2461 agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg cgaagcacct
     2521 tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg attattttgg
     2581 agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg aagataatcc
     2641 aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc gtaacccaga
     2701 acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat tttaagatcg
     2761 ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc tactttatac
     2821 gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa acggtggaat
     2881 cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat gatgaagagg
     2941 taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc atcgaaatac
     3001 gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa caaagcgctg
     3061 tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt tatgttgctt
     3121 tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc ccgaccggcg
     3181 atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat tcggaaatca
     3241 ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt tcaatgatcg
     3301 tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga gaacaagcca
     3361 ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa gatgaacttg
     3421 gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct caagatacaa
     3481 tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac ggcgtcattg
     3541 cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc ttgctagata
     3601 tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc gaagaagaat
     3661 atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt tcaaccagta
     3721 cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt cgcgaatcag
     3781 gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa aaaacagaac
     3841 gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg ttgacaagta
     3901 tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa gtagcaccaa
     3961 attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc aatgacctct
     4021 taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat gtcaatttca
     4081 acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat caggaaaaaa
     4141 attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta gatacagata
     4201 aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca ccagatgggg
     4261 gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt atcaccgatc
     4321 aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc tatcgagtag
     4381 ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt tctagagaag
     4441 tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa gggtcgactt
     4501 tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga gaatctctga
     4561 aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact tctcttatgc
     4621 aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa acactcaaat
     4681 gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc atgttacatg
     4741 gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc gattgcaatt
     4801 gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag acaaatcaca
     4861 gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg cgcctttttt
     4921 gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact tggcaaaaga
     4981 aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa aaatccgatt
     5041 tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt ggacagatat
     5101 caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag aaccatcgac
     5161 gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata gttatatcct
     5221 gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag atagcgtgaa
     5281 aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa tgtctatcca
     5341 ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag aaaatgctag
     5401 tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag ggaacctcag
     5461 atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag gcttcccggt
     5521 ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa cgaatggttc
     5581 tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac cttcagacga
     5641 agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta agggagcaga
     5701 tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca aagaaacgaa
     5761 tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact ggtattcagc
     5821 taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt tcaaaaaaat
     5881 cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca gcagttatca
     5941 tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat caatccaaca
     6001 acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga acaagcaagg
     6061 ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt atcgcaaggc
     6121 tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt tgacgaaatc
     6181 tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta gtagttcctt
     6241 ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg tgtacacgaa
     6301 aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag agtatgaagg
     6361 catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa acgataatga
     6421 ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc tgcgcgaaaa
     6481 gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata acaaaatacc
     6541 aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga aagtccggga
     6601 aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata aagtggtcca
     6661 aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg taccaaaagt
     6721 ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga agggaagtag
     6781 gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa gtggaagttc
     6841 gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg caggacttag
     6901 cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc aattagatgg
     6961 aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct tggctcgtaa
     7021 gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac cactgattgg
     7081 aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa cattcggcga
     7141 tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat tttatcgttt
     7201 ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca gtgattatat
     7261 gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg atcttgagat
     7321 gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag atagaggaca
     7381 tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc ggaccaaacg
     7441 aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc atttaacaat
     7501 ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa tctatgatac
     7561 agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa taaaaatata
     7621 aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat ttttttagca
     7681 tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt tcatgtttct
     7741 catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc gtactgtcgc
     7801 cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta cgtttgccat
     7861 gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta gattgtttca
     7921 attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca tttttaggta
     7981 cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag gcacccccaa
     8041 cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac aaagaagttt
     8101 gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca tcaaaagatg
     8161 caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca tctgatggca
     8221 atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat gcttcgtcta
     8281 aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata aaaaacctcc
     8341 ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat ccgatttttg
     8401 aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt aaattaagaa
     8461 aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg ttagatcgtg
     8521 cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct ttgcgtaatg
     8581 gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta tttgctcatt
     8641 ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg tattttacac
     8701 aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc attgcatacg
     8761 cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc ttgtctgctt
     8821 atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca ggtgctgaag
     8881 ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca cgtttagtaa
     8941 gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg cagtctttta
     9001 cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca gcggtttttc
     9061 aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag cttcccattc
     9121 aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt ccgtttttat
     9181 ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc gtcttgcaag
     9241 cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg gtggcaaatg
     9301 gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg ggtgctggat
     9361 tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa tacagagaat
     9421 taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt attttttcca
     9481 caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg gtcgtttcag
     9541 gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca gcagtccaag
     9601 tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg cctgcagcga
     9661 ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt ttgaaaaaaa
     9721 tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca gcagaaaaga
     9781 tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc accaatctag
     9841 gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc taattgaaca
     9901 agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat ccgaccagtt
     9961 gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag atgaccatca
    10021 ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa atttgaatat
    10081 cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat gggtccagca
    10141 acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat attttgtgta
    10201 ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc gatgctttgc
    10261 agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg agcagaagct
    10321 ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa aatagctagg
    10381 acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga cgtagatttt
    10441 acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata gcaaaaaatg
    10501 ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga taacaagcat
    10561 gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa gcagctgtca
    10621 gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa tgagtattat
    10681 taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa aagagttatc
    10741 ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag tcgcaaaaga
    10801 agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga aaccccaaac
    10861 tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa aagttcctgc
    10921 cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag aagtaaaata
    10981 cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg ttgagtaaat
    11041 ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat ggcaaaagaa
    11101 ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa attagcagat
    11161 acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa atcttacggc
    11221 tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt agaagatcat
    11281 tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa tgatattgca
    11341 ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga aggtttaaaa
    11401 aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt ggcaacaaaa
    11461 gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga agccattgca
    11521 caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc tgatgcaatg
    11581 gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat cgaaacagaa
    11641 ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata tatggtaaca
    11701 gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac agacaaaaaa
    11761 atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca atcacgtcca
    11821 ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt attgaacaaa
    11881 atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga tcgtcgtaaa
    11941 gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga cgatctaggt
    12001 ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt tgttgtagat
    12061 aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga agctcgtgtg
    12121 caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga aaaattacaa
    12181 gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc agctactgaa
    12241 acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac acgtgccgca
    12301 gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat cagcaaagtt
    12361 tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt tcgtgcttta
    12421 gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt gattgttgat
    12481 aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga atgggtaaac
    12541 atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt gcaaaatgcc
    12601 gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa accagaacca
    12661 gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat gtaagaaaaa
    12721 gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag gctggaacag
    12781 aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca aatttttgtg
    12841 aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg tgtttagagc
    12901 gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta attataatga
    12961 aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat ccattca
//
LOCUS       DACIRY010000070        12973 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.071, whole genome shotgun sequence.
ACCESSION   DACIRY010000070 DACIRY010000000
VERSION     DACIRY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.071"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(266..985)
                     /locus_tag="IYE22_001744"
     CDS             complement(266..985)
                     /locus_tag="IYE22_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ9506953.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1034..2680)
                     /locus_tag="IYE22_001745"
     CDS             complement(1034..2680)
                     /locus_tag="IYE22_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ9506954.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2859..3449
                     /locus_tag="IYE22_001746"
     CDS             2859..3449
                     /locus_tag="IYE22_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9506955.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3541..5046
                     /locus_tag="IYE22_001747"
     CDS             3541..5046
                     /locus_tag="IYE22_001747"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ9506956.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5130..6482)
                     /gene="celB"
                     /locus_tag="IYE22_001748"
     CDS             complement(5130..6482)
                     /gene="celB"
                     /locus_tag="IYE22_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ9506957.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6482..7000)
                     /locus_tag="IYE22_001749"
     CDS             complement(6482..7000)
                     /locus_tag="IYE22_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506958.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7016..7342)
                     /locus_tag="IYE22_001750"
     CDS             complement(7016..7342)
                     /locus_tag="IYE22_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ9506959.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7355..9334)
                     /locus_tag="IYE22_001751"
     CDS             complement(7355..9334)
                     /locus_tag="IYE22_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ9506960.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9355..9669)
                     /locus_tag="IYE22_001752"
     CDS             complement(9355..9669)
                     /locus_tag="IYE22_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ9506961.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9837..10130
                     /locus_tag="IYE22_001753"
     CDS             9837..10130
                     /locus_tag="IYE22_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ9506962.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10208..11320)
                     /locus_tag="IYE22_001754"
     CDS             complement(10208..11320)
                     /locus_tag="IYE22_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ9506963.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11473..11697
                     /locus_tag="IYE22_001755"
     CDS             11473..11697
                     /locus_tag="IYE22_001755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506964.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            11707..11886
                     /locus_tag="IYE22_001756"
     CDS             11707..11886
                     /locus_tag="IYE22_001756"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506965.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12080..12250
                     /locus_tag="IYE22_001757"
     CDS             12080..12250
                     /locus_tag="IYE22_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9506966.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12674..>12973)
                     /locus_tag="IYE22_001758"
     CDS             complement(12674..>12973)
                     /locus_tag="IYE22_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9506967.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4090 a         2610 c         2205 g         4068 t
ORIGIN      
        1 tatcagaggt tgtgaattca gcgttttggc ctgagtatta gggagatcgt gtccttacta
       61 tctaactctg agtcacaagg aacaatgggg actgtaacaa aagtcttgtc acagtccctt
      121 attccgaaca aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa
      181 aaagccatcc gaaagtcaga cggacaagtc tgcttttcag atggcgctac aactactctg
      241 cttattcttt tagctatcca ttcaattatt tttctctcac ttgttctagc tcttctttgg
      301 tcatgggtcg atattgccct ttattcaaat ctttaggcag ccaaaatttt cccatacgga
      361 ttctttttaa atataagact tcttttccta ctgctttgac cattcgctta acttggtgaa
      421 actttccttc atgcaaaatc agacggataa ttgaagtttc tgtctcttga tctacttgat
      481 caatttggag ttttgccggc aatgtctgct catcgccatc aataatcagt ccttctgcaa
      541 ataaacggat atcttcttct gtcattatac cgctaacctc agctagatat tctttctcca
      601 catgtttttt aggtgatagt aactgatggg caaacacccc gtcattcgtt aaaatcaaga
      661 ggccctctgt gtctttatct aatcggccga ctggaaaaag atcttcccga taatcctggt
      721 cttccaataa atcgattacg gtttgatcat gattatcctg agtagcagaa atgactcctg
      781 caggtttatg caaaatgtag taaaaatctt tttgatacgt aatgatttct cctaaataag
      841 taaccgcatc ttcgaattca tttacttgcg ctttatcgct tttcaccact tgtccattga
      901 cttcgatcgt tccttttttg attaatcctt tgacttcttt gcggctgcca agtcccatat
      961 ctgctaaaaa cttatctaaa cgcatgcgtt tcccccattt caaaagagca tcaatctagc
     1021 tcttctgatt cacttatttt atttttaatt tatgtcgtaa acgcgctatt cctggtccta
     1081 gtagtctttc tgccaagcgg attttcaaag ctaaataacc atacactaaa gcccctatac
     1141 ctgctactac aagaatcaac aatagagact ggaatttgct atcttcgttt aaaaaagcac
     1201 caaaaatcat ctttgtgatc cacgaagcca aaagcataat caaggtaaga ataaagatca
     1261 gcaatgtcct gcgccagaca aaacttggtt taaagcgggc tacttcatgg attctcttta
     1321 agatcaaggt acaagacaca gcaaatccga tcattgtcgc caataaaggg ccgtatactt
     1381 caaataaacg aatggctggg tattgaagaa ggagtttcac tagaaaccca ataaccaaat
     1441 aattaattgc cgccttgttt tcaaacattc cttgcagcat gttggaaact agcatatata
     1501 gtcctaggaa gagtcctaca aaacttgctt ggatcagtac attacttcct agagaatccg
     1561 gtgtataaaa taatgtattt agcggatagg caagtaacat aacaccaaat gtcgccggaa
     1621 acataaaaaa tgaaaacaac tgtaaatttc cgcttgtcaa ttttgccaga cctactcgat
     1681 ctttcaacgt aacagcttct gtaatcaacg gtaacccagt agacgcgatc gatgttgcca
     1741 atgcaataac taccatcgtc aacttatctg gattggcact aaaaatcgaa aaaagatcca
     1801 gtaactgtgc attggaatac tcggtggatg cagacatgat cttcataaaa gtgaactgat
     1861 cgactaattt gaagatcgtt acccctgaac ccacaataat aaacggaatc gcttctttaa
     1921 cggtatctaa caacaattcc ttcgttgcaa tatttatttt gttttcgctg tagtgaataa
     1981 acgccgatgt atacgcttga tgtttcttca aaaaatacaa aaggacaaga atacttgcta
     2041 gcataccgat aaatgccgcg aaagttgatt gcgtgaccgc cgtcacatag tcaccatcca
     2101 tgactttcat gatgatgaat gtagccaata gcatataaaa aatacgtgcc acctgttcta
     2161 caatctgcga cagcgcataa ggcatcattt cttgattgcc ttgcaaatac cctcgtatca
     2221 cactcataca aggaaaaacc aatacagcga tactcaaaga acgcatcgtt ggaatcagtt
     2281 cttctccgcc accagaagca tgagctagcc aaggagaggc gatgtacatg aacaaagcaa
     2341 acaacaggcc taacccgccc atcatctgta atgcacggat aaataatcgt cgtgaagtgc
     2401 catattcatt caatgaatta tagcgtgcag tttgttttgc aatcgcagca ggtattcctg
     2461 ctgtagagat cagtagaaac aatgcatata tattgtatcc catattgaat aatccattgg
     2521 cagccttagc attttcgccc atccaagcat accagggaat gatatagaca gcgcctagca
     2581 atcttgaaat gatattactg gcggtcatcc aagcagagcc tcgtgccatt ttttcttgcg
     2641 gtgttaattg atctaatgct ggttttcttt gattatccat cggtcagatt ccaactttct
     2701 acaaaacatt actatcattt taattggtaa aacagaaact ggcaatcgat tttatataac
     2761 ttttataaga acaattgttt ttttgaattc ggtttcgttt ttttggtaaa gtaaagaaat
     2821 atacagcctt ttatggctaa catttatagg agatatcaat gacacaagaa ataaaaaaac
     2881 tacccgttat atttttaggt cttttcttta tcacagttgg attgaattgg ttcttgctcc
     2941 ctcacgatat agcttccgcc ggagtgggcg ccatcggaca tttagttgaa acggcctttt
     3001 ctatccaacg ctcagttact gtttggagca tcaatcttat gatgctgctt ctagccgctg
     3061 tcctattagg aaaatcagta ttttttaaga ctgtagcggg aagtctgctt tttcctgttg
     3121 ttttagagat tgttccaaaa gtggaaatga tctcctcaca tttcttttca ctgatttcag
     3181 gaagtctttt gttcagtctt ggggtttaca cattgtacac agtcggggcc tcaaatggcg
     3241 gtataacgat accgccgatt attttccaca aattttttcg gcttccaatg ccgcaaggac
     3301 ttttacttac caatagtctt atcgtctttt tgaattatta tgtttttgga ttgttggaga
     3361 cactattcgt tctcctatct attacagtca gttcattgtt tatgaaaata ctcatccgct
     3421 tgtcccctgt gtcaagaaaa tttgaataaa gctggatttt gtcatagcgg aaacaaacga
     3481 tatattaaaa agagtgtgct ataataaacc aagaaattga ataaagcgag ggatcaatca
     3541 atgagcttct ctttagaaga catccgtcat ttactattaa aagaaaatct attaaaagaa
     3601 tttgtttcaa caagaggctg gcatcttgat gtaccagaaa aagctgaatt tcttcagtta
     3661 agttatgatt caagaaaagc tgatgcttct actttgtttt tctgtaaagg aatgaatttc
     3721 aaagaagaat atttggattc agcaatcgaa cagggtattc aatattatgt atcagaacaa
     3781 ccatacgaaa attcagcagt aggaatcatc gtaacggata tccgtaaagc aatggcgctt
     3841 cttgctatgg ctttttacga ctatccgcaa aataaattga aagtgatcgg attcactgga
     3901 acaaaaggta aaacaactgc tgcgtacttt acaaaagcaa tcttggatca cacaacaaat
     3961 aaaaaaacag cattgctttc tactatgaat acaacgttag atggcaaaac atatttcaaa
     4021 tcccatttga caacgcctga gtcattagat ttatatcgta tgatgtcaga agcagttgaa
     4081 aatggtatga ctcatctagt gatggaagtt tcttcacaag cttacaaaac acaacgcgta
     4141 tacggcttga ctttagatgt cggaatcttc ttgaatatct cgcctgacca tatcagtccg
     4201 atcgaacatc cgacatttga tgactatttc tattgcaagc gacaattgat cttgaattca
     4261 aaaacagttg ttttaaatca tgaaagtgac tattttgatt tacttaggga aacagccgat
     4321 ctattccaga ttccaacgat cacttatggt cgttcggaag aaagcaatta tcaagtgatc
     4381 cgttcagata aaggtactca cggttttact ttatcttctc atgaagatcg cttagctatc
     4441 tgcgatactg cttatgatat tttgctggct ggaggattca accaagaaaa tgcagccagt
     4501 gcgatcattg ccgcagcact aacaggtgcc tccagtcaag atgcacaaga cggattaaaa
     4561 gaagctcgtg taccaggccg aatggatcaa ttgatccaat ccaatggagc gcatgtctat
     4621 gtggactatg cacataatta tctaagcttg aaaacattgc tgaaatttgc caaaaatgaa
     4681 catccagatg gccgtgtaat tgttgtatta ggcagcccag gaaacaaagc aatctcccgt
     4741 cgacatgatt ttggaaaagt gctatcagaa acagctgaca tcgccttttt gacggcagat
     4801 gacccagcat ttgaagatcc gaaaaaaatc gcggaagaaa tcaatgaagc aattactaat
     4861 ccagatttga tcgttcatta tgaaatggat cgtcctgaag cgattcgacg cgctttagca
     4921 gaaagcgctc cgcaagattc agtcgttatt gcgggaaaag gcgtggatcc ctatcaaaaa
     4981 atcaatggtg tagatgaacc ttatgaaggg gattatgcaa tcgtgaagcg gttgattgaa
     5041 gaataatggt atttatatac tagtggtttc ccatttctaa gaaaaaaagg gactgtgatt
     5101 ttttgtcaca gttccttttt tcttagaaat cattttaact tttcactagc taattctgct
     5161 gccttttctc tttcttcatt taataattta ttatcataga atttgataaa tgggaaccag
     5221 ataatgaatg cgatgattgc acaaatgact gctaacacag cagctttcca atcaccacca
     5281 ctaccaacaa atgctccgat cccgactggt gaaggccacg gcatctgtgc aagcatcggt
     5341 ttgacgattt ccagccgaat ggcaaaataa gcgagtgtag cagaagccat cggagctaag
     5401 aaaaacggaa ccgctaaata gggattgtag acgattggca tcccgaaaat gattggttca
     5461 ttgatattga ataatcctgg tacaagtgag gcttttccta acactttcag ctgatcagat
     5521 ttcgcgagga atgcaataaa gatgatcaat cctaaagtcg cacctgatcc accaacggtt
     5581 acataagaat tgttgaattc tcctgccaac ggataatttg cgccttgtgc gttagctgcc
     5641 atattagcta atacgatcgg ggtcaagaaa gaagtgatga tattcgctcc atggatccct
     5701 acgatccaca atgcatggat caagaaataa atcaccatga caccaagcca tgtattcgtc
     5761 aactgcgtaa caaacccaaa tgggatagca atcattttga agatatccgt tcctagtgca
     5821 actaataatc cattgatgat catcaccaca aacgcaatga caaatgttgg gatcagagca
     5881 gtgaaagaac gagaaactcc tggcggaaca acatctggca tttttatgac ccaatttctc
     5941 ttgacacaca tacaatacaa ttcgaccgca ataatcgaca taataatagc tgtaaagatt
     6001 cctgaagttc ctaaacgttc gacaaaagag cccatcctaa taccatttac tacagtttct
     6061 gtctcattga tagaagcaat caagctgatt ttaccatcag tcatgatcaa ttcagggata
     6121 cacataaaga atgcaaaaac agatagtaat gcccccgtga gcggattaac atttaatttt
     6181 tgttcatctc gttcaattga cgttaattca taagcaaaca caatggcaaa gtaaatagat
     6241 aaaatcccca tcgtcattgt attgacgatc atgtacaagt cactaacttt gaaaaaagtt
     6301 gcattaaaaa atccttctaa aaatgggaac ggtaatggca aaatatttaa aaccaaaaac
     6361 atcgagccta caatcgtgaa aggaatagaa gccatccccg ctgccatgac agcccgaacg
     6421 attttaagtt gcgcaatttt ccccataggt cccatcaggt atttctgcaa aaaatcaaac
     6481 attagttatt ctcctttcct aaatataacg ctttttcata ttgtttaata cggatatact
     6541 gttggtcttg gttgagtgaa attttcttta aacgatactg tataaatgca ctggcaaaaa
     6601 taccgcttac aacaagaatg aaaacaaata actgtcctcc attgttattt gccataaaag
     6661 gatacaagaa tgtaaatcct gaggtaaaca acactggaat cactaataaa ttaaagacac
     6721 tctggaacaa aaaataccac tttgtccatg gaacgttcgt ttggtgtttt cgataaagct
     6781 ttacttgttc tcctactgca ggcaaaatag ctaaaatcag aatcatcggt aaactacttc
     6841 ctaaactttt agctgataga agcagaatca tccattgcat gttcatgaaa aagaaaaagg
     6901 cagtcaggta acgtacgatt agataacgat tgaaatacat gtttttcaaa ctaagttgct
     6961 ttttttctaa ttcgattttc ctttttactg tatcctgcac aatgatcctt cctttctatt
     7021 tatttttttg atacaaataa ttcatttcct gtgcaacttc caataatgtc atcgtcgtca
     7081 tcagatgatc ttgtgcatgg accatgataa tttccatttc tatttttgtc ccgctggcat
     7141 attctttcag aagttgagtt tgtgactgat gcgcatgaag aatctcttca tttgcttgtc
     7201 gcaacttctc ttctacttct tcaaacatct ctttacgcat cgagcgaaat gcttcatgta
     7261 tcagtgtccg tgcatttccg ctatggaaaa tgatatcaaa agaagtcact tgtagtgttt
     7321 cactgtttat tttttcagcc atctgttttc ctccttattc gatcagtttg ataaatttac
     7381 tgctgaaatt ctcaaaaata ggttcagcta agatttcctg ctgtactttt agctggtcaa
     7441 ctaacctaac gattgcttta gtcacgaccg taattccttc attttcgaca taagaaggcg
     7501 aaactaaaaa aacaaaatga atttcttgat attgatccca ctgcatccca gaaggaatca
     7561 atgcaacggc tacctttgtt tcgattccaa ctggaacagc gggatgtggg actgccactg
     7621 tatcactgaa tatgatttgt cccatatttt cacgttgctg gatctgcttt cgcatttctc
     7681 ttatatatcc ttcttcttca tttatgctca gcttttgcag caaatcatga agaatttctt
     7741 cctttttcgg ttcgtacgta taagtcttga aaaatttttc agacaactgt tcggtgtaaa
     7801 tctgcttttt ttgctgtaga gaaaatgctg gtatcggtct atctgaggaa tgatagggta
     7861 tagattctcc aactaccttt cgtattttac gaacatcatc ttcattcaaa aaaatactga
     7921 catgtaaaac tggaattttg aaaaacattg tcgaaagatc aatagaagaa ataatcaggt
     7981 cgattccttt taaagttgtt tcattgatct cataatagcc ttttacactt accactgtaa
     8041 tatttttacc gaattcactg actacgcgat tctttaatag ttgggcactg ccgtaacccg
     8101 ttgcacagat aatcaatgca tgtagtttat gcgcagctct ttctttttcc aatgccgcca
     8161 tgagatgcaa agccaaatag gcccattcat cttcattgat ggtatagttt ttcaaagccg
     8221 gcatgttgct gaaatgcctt tttactgcgt caaagacttt gggattttct ttctttatct
     8281 cctcagtcaa tgggttctca atgcgaatac ctcggctcaa tcgaatcatc atcggtgaca
     8341 aatgatcgag tagtccattg aaaagctgga gatcctctac taatgaaaaa tgaaattcct
     8401 gagacaatct tttcagaact ttcgctaatt cctttgatag ttcctgatca ttttgatttt
     8461 ctttgtgatt cgacttagcc atgagatgta atgtcaaata agcaatttct tcttcgggaa
     8521 acgatacacc tgcgattacc tcaatacgtt gaacgatccg ctgcgccact tgatactcta
     8581 ctcgcttaga aacttcttgt tcgatgccta attccttgat ttcaaaccct tctcggagcc
     8641 gtttgatccc caaagccaag tgtaaagtca gattctgtat gataaaatcc gaaactttta
     8701 acttagcttc tcttgtttca tcgagaatga taatgactag ctcttcaaaa ctgatgtctg
     8761 ggaaaaaacg gctttgccct aaatattctt tcaatgaatt tccataatgg tttccaaaaa
     8821 aagtatccat gataaaatgc cgtcgatttc tttcttcccc ttcgatccat gtcccttgac
     8881 catgcttaga aacgattttt agtgaatatg gaatcaatcg ctccttgatt tcttgcatgt
     8941 cctttgtcaa actggaacga ctgatataaa gctcttctgc caaatcatcc ataaaaagaa
     9001 tggcatcttc caaaagcaat tggttcaata tatacttttg tcgatcttcg gactcataaa
     9061 atccttgcgg ttctctagca tatccaggta ctatttcttg ccgactcaaa aacaagtcaa
     9121 atgtcagtct ttgtaaaata tgcagttggt agccataacc tggtttagca atgatcttac
     9181 ctccgttttt ttctactaac tctttgatcc gctgcaagta attacggatt gtccgatcag
     9241 ataataacaa ttcagaagct aaacgttgac tggtgacata ctccttcttg tgatcaatca
     9301 aatatctcaa cagcaatttt tccttctgtt tcattatcat ccactcctat tccatcatat
     9361 gttctcttcg atcacctctg ctaacttctg gatgcctgta gggattggga catacgcttg
     9421 gaaagggata ctaacaacag gctttccaac agattctcct aattttttga atttatccaa
     9481 aaacatcgtc gtttgcgggc tgatcagaaa taaatcaaaa tcactattct taatcatatt
     9541 atcgccttct gtagctgaaa ctgcatctac gagtacctct tctctttttt gcttaaagaa
     9601 atccgttgtc tttgaggcca tcatcgagga tgacattcct gccgcacaaa taatcaatgc
     9661 ttttttcata aatcaatcac tccttttatt tttataaacc aagcatatct tgtaagcgtt
     9721 ttctattcaa aatatatatt tccgtagcat tgcggaaaaa atgtgtatca tatcttgaat
     9781 aataatttct ggcaccgtga tatactagtg atagaatttt ttcaggaggg gatttagtga
     9841 gtatcacagt gaatattttg tatacaagta caaatggaca agccagaaaa tttgcagaag
     9901 aaatggtcga aagaaacatt gtcaaagcga ttcgtgcaga agaaggaaac gagaaatatg
     9961 actattactt tccactagaa gatccagaat ctcttttact gatcgaccgt tggaaagatg
    10021 aagaagcaat cgaaaagcat cacaaatccg aaatgatggc tcaaataaac gaactccgta
    10081 aaaagtataa attaaaaatg aaagtcgaac gtttcactga tttaccgtaa aaagatacac
    10141 atggaatgat agcaatttat catccatgtg tatcttcact ttttccttat tttttattcg
    10201 atctcatcta atgattcaaa ccgtgttttt ctcttatttt tcggataagt atttcagtca
    10261 ccccaatata catgattcct aaacataccc caaggatatt ctcaaaaatg cggatcgtta
    10321 ctgctcctgg aaccccgaat aaactggcag tgattgccaa agcaccaaaa gaattaaaaa
    10381 ctgttttata tctataggta gaacaaacac ctaaagctag accacctaat ataccgaccc
    10441 agttaaaagg aataaattgg ctgataccaa taaataaaac acatccaatc aatgagccaa
    10501 tgattcggtc tactgctcgt tctttgaaac caatcgacat caatccatag ctagatacaa
    10561 tagaagaaaa tgcaaaccct gcccacatca atcgctgaaa agggacatac tctcctgcaa
    10621 cgatcaaaag actaatgcca aatgcataga tgagcatcca aattttttcc tgcgaataca
    10681 tgcctttcaa aaaaatctct ttaaacaagc ttttgtcttt gttcttttct cgatgttttc
    10741 tgtaaaggag aactgaaaac caacaaaaaa acaaaaataa taagctactt gtttgtgtaa
    10801 agaaattctt gttcaacgaa tctttaggta acgaataaac aataaagaga tatgaaaagc
    10861 cgtacaaaga agcattcccc attttgggat cactagcggt tgccattaat atgctgctta
    10921 gtaaaataaa atgaatcaac atgcccataa ctgaccattt gatcagctgg acatacggtg
    10981 ctactaatag tgagaacatg acgattgcta aacctatgat cgaatgggaa atcttataac
    11041 caaagtcaac aaaacgaata ctcatcaaca tacagaataa aaccacagcg tatggtttat
    11101 gcttcgattc aaataatgtt gtaattataa tgataaaaca aatggcaaac aatacgatga
    11161 ataatgatcg taaaaacaat gaacaaatat acatgcactt ttctttttta gaagctgctt
    11221 ctcgtatttt ttgttttaaa ataaacggat ctagttgtag agactgataa aaatccacct
    11281 gttgtcgtcc tttcttttta cttttatttt ttacagtcat ctatgacagg ttgtaaatac
    11341 tatggaacat catatggcgt tgtcattttc ttgtcaacta tttttcctat tcatgttttt
    11401 ataaaaaaac atatcgtttt cttgtatgat taaatcacca caaaataaac aactgagaga
    11461 acgatatgct ttatgaatgg ttctatcaaa aaaattctga gatcaataga caaaaatttg
    11521 atgattacag aggtctctta cgaaacctta cagaagaaaa aaacattgat cgttgctgca
    11581 gttctcttcc ctgctccatg tgtctgtaga aattgtggtt ctgctgtggt agatggaaat
    11641 aggaaattaa tggtggtgaa aaacaagaag aaggaaacca ttgtccactt tgaataatat
    11701 aatcatatgc caatggttat acgcctaaaa aagcagcgct atacctgtaa aaactgtcga
    11761 agccattgga acgctcaaag ttattttatc cgtccaaggc actcgatttc aaatcatgtc
    11821 agacataaaa tcacttcttt actgactgaa aaagtgtcgt tattttttat ttcgaaaagt
    11881 tgttaggtat ctttgacaac cgttattcgc acattgaaag agttgagaag ctatttacca
    11941 aagtcttcca aaagaattct gtcttgagtg ttgagggttg tgaatttcgt tctcacgttt
    12001 ccgttgagga taaactgagt tttatttccg cggatggcga gacagaaaaa ttaatcgaga
    12061 aaatttacct acacaaaaaa ttcctcgatt aacaaactat ttccttagct gcactaatcc
    12121 taaagaaata gaattcttag ttacagacat gaacgccacc tactttcagc tcaccaaaca
    12181 tgttctacca aatgcgaaat tggtgattga tcgattactt ggtttttcca cgcctttcaa
    12241 ggaggcgtaa cctttattca cgagttggtt gaagctttcc gagacaaaag acccgacttg
    12301 tttttctcct tattgacaaa gagccattaa aactagagaa aaaattacaa ctgatcgctg
    12361 attatttgta gaaagaaaat caaagtatta cccaataaac ttgagtcatt aggcaaaaaa
    12421 cactgcgaaa ctcaagaatg attttcgcag tgtttttgta tattttttat taactagtat
    12481 tactacaaag gtttattttt cacttctcaa cgcagcacaa aattgtcttt acttatttac
    12541 tggttgggtt caaccttctc tactccgata ctaatatttg tttgtatacc ccttctaaaa
    12601 tcggtagttt ccattcaatt gatgggtact agttgatttt aaaaactatg cttattcaat
    12661 tttttatttt agctcatata tttcgtttct ttctaaattt tatactgatg aaaacaagta
    12721 gcacacccaa tattgacaat ataaactggc taatgatggt ttctccagtt tttggaagat
    12781 gcttatttcc actgcttgtt ggattgtttc ctcttatatc tgagctatca gcaacttttc
    12841 tattctcttt tgttaaaaag atcgccactt cttgttcttt ttcgatcttg aaagttactg
    12901 gtgtcgcatc caattcatac cccgttggcg cttgagtttc taccagctga tatgctcctg
    12961 gtgctaagcc atc
//
LOCUS       DACIRY010000071        12945 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.072, whole genome shotgun sequence.
ACCESSION   DACIRY010000071 DACIRY010000000
VERSION     DACIRY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.072"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="IYE22_001759"
     CDS             complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="IYE22_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ9506968.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1197..3038)
                     /locus_tag="IYE22_001760"
     CDS             complement(1197..3038)
                     /locus_tag="IYE22_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ9506969.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3220..5613)
                     /locus_tag="IYE22_001761"
     CDS             complement(3220..5613)
                     /locus_tag="IYE22_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ9506970.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5720..6580)
                     /locus_tag="IYE22_001762"
     CDS             complement(5720..6580)
                     /locus_tag="IYE22_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ9506971.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6639..7271)
                     /locus_tag="IYE22_001763"
     CDS             complement(6639..7271)
                     /locus_tag="IYE22_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ9506972.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7480..8199
                     /locus_tag="IYE22_001764"
     CDS             7480..8199
                     /locus_tag="IYE22_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9506973.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8225..10816
                     /gene="mprF"
                     /locus_tag="IYE22_001765"
     CDS             8225..10816
                     /gene="mprF"
                     /locus_tag="IYE22_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ9506974.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="IYE22_001766"
     CDS             complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="IYE22_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ9506975.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12559..12915
                     /locus_tag="IYE22_001767"
     CDS             12559..12915
                     /locus_tag="IYE22_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ9506976.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2709 c         2299 g         4224 t
ORIGIN      
        1 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
       61 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
      121 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
      181 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
      241 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
      301 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
      361 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
      421 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
      481 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
      541 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
      601 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
      661 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
      721 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
      781 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
      841 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
      901 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
      961 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     1021 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     1081 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     1141 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     1201 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     1261 tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     1321 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     1381 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     1441 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     1501 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     1561 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
     1621 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
     1681 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
     1741 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
     1801 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
     1861 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
     1921 cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag atattcatca
     1981 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
     2041 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
     2101 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
     2161 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
     2221 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
     2281 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
     2341 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
     2401 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
     2461 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
     2521 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
     2581 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
     2641 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
     2701 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
     2761 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
     2821 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
     2881 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
     2941 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
     3001 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
     3061 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
     3121 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
     3181 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
     3241 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
     3301 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
     3361 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
     3421 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
     3481 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
     3541 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
     3601 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
     3661 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
     3721 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
     3781 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
     3841 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
     3901 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
     3961 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
     4021 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
     4081 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
     4141 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
     4201 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
     4261 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
     4321 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
     4381 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
     4441 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
     4501 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
     4561 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
     4621 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
     4681 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
     4741 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
     4801 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
     4861 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
     4921 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
     4981 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
     5041 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
     5101 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
     5161 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
     5221 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
     5281 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
     5341 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
     5401 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
     5461 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
     5521 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
     5581 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
     5641 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
     5701 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
     5761 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
     5821 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
     5881 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
     5941 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
     6001 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
     6061 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
     6121 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
     6181 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
     6241 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
     6301 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
     6361 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
     6421 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
     6481 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
     6541 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt
     6601 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
     6661 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
     6721 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
     6781 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
     6841 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
     6901 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
     6961 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
     7021 tgcagacttt ccgagctctg catgagatct gtcccttttc cttttgattc ggtcaatatt
     7081 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
     7141 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctcc taaaaacaac
     7201 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
     7261 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
     7321 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
     7381 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
     7441 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
     7501 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
     7561 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
     7621 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
     7681 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
     7741 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
     7801 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
     7861 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
     7921 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
     7981 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
     8041 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
     8101 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
     8161 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
     8221 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
     8281 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
     8341 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
     8401 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
     8461 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
     8521 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
     8581 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
     8641 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
     8701 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
     8761 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
     8821 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
     8881 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
     8941 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
     9001 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
     9061 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
     9121 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
     9181 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
     9241 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
     9301 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
     9361 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
     9421 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
     9481 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
     9541 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
     9601 cgttattggg gtatacaatc tgccagattt ccctcatcgg cgtcaccatt ttatttcctt
     9661 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
     9721 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
     9781 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
     9841 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
     9901 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
     9961 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    10021 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    10081 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    10141 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    10201 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    10261 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    10321 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    10381 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    10441 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    10501 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    10561 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    10621 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    10681 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    10741 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    10801 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    10861 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    10921 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    10981 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    11041 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    11101 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    11161 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    11221 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    11281 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    11341 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    11401 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    11461 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    11521 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    11581 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    11641 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    11701 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    11761 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    11821 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    11881 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    11941 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    12001 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    12061 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    12121 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    12181 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    12241 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    12301 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    12361 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
    12421 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
    12481 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
    12541 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
    12601 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
    12661 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
    12721 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
    12781 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
    12841 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
    12901 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaa
//
LOCUS       DACIRY010000072        12901 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.073, whole genome shotgun sequence.
ACCESSION   DACIRY010000072 DACIRY010000000
VERSION     DACIRY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.073"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            133..3660
                     /locus_tag="IYE22_001768"
     CDS             133..3660
                     /locus_tag="IYE22_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ9506977.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IYE22_001769"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IYE22_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ9506978.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IYE22_001770"
     CDS             7448..7789
                     /locus_tag="IYE22_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9506979.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IYE22_001771"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IYE22_001771"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ9506980.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IYE22_001772"
     CDS             9146..11566
                     /locus_tag="IYE22_001772"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ9506981.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IYE22_001773"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IYE22_001773"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ9506982.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4379 a         2241 c         2738 g         3543 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 t
//
LOCUS       DACIRY010000073        12723 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.074, whole genome shotgun sequence.
ACCESSION   DACIRY010000073 DACIRY010000000
VERSION     DACIRY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.074"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(287..649)
                     /locus_tag="IYE22_001774"
     CDS             complement(287..649)
                     /locus_tag="IYE22_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506983.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IYE22_001775"
     CDS             complement(723..1103)
                     /locus_tag="IYE22_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ9506984.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IYE22_001776"
     CDS             1185..2360
                     /locus_tag="IYE22_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ9506985.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IYE22_001777"
     CDS             complement(2437..3021)
                     /locus_tag="IYE22_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9506986.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IYE22_001778"
     CDS             3222..4220
                     /locus_tag="IYE22_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9506987.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IYE22_001779"
     CDS             4332..4841
                     /locus_tag="IYE22_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ9506988.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPSILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IYE22_001780"
     CDS             complement(4871..5530)
                     /locus_tag="IYE22_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ9506989.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IYE22_001781"
     CDS             complement(5532..6296)
                     /locus_tag="IYE22_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ9506990.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IYE22_001782"
     CDS             complement(6304..6981)
                     /locus_tag="IYE22_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ9506991.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IYE22_001783"
     CDS             complement(7018..8403)
                     /locus_tag="IYE22_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ9506992.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IYE22_001784"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IYE22_001784"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IYE22_001785"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IYE22_001785"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ9506993.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IYE22_001786"
     CDS             9342..10199
                     /locus_tag="IYE22_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ9506994.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IYE22_001787"
     CDS             complement(10280..10615)
                     /locus_tag="IYE22_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9506995.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IYE22_001788"
     CDS             complement(10682..12052)
                     /locus_tag="IYE22_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ9506996.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IYE22_001789"
     CDS             complement(12130..12666)
                     /locus_tag="IYE22_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9506997.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3789 a         2581 c         2117 g         4236 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct agtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaa
//
LOCUS       DACIRY010000074        12684 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.075, whole genome shotgun sequence.
ACCESSION   DACIRY010000074 DACIRY010000000
VERSION     DACIRY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.075"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(107..550)
                     /locus_tag="IYE22_001790"
     CDS             complement(107..550)
                     /locus_tag="IYE22_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9506998.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(566..1726)
                     /locus_tag="IYE22_001791"
     CDS             complement(566..1726)
                     /locus_tag="IYE22_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9506999.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2233..3933
                     /locus_tag="IYE22_001792"
     CDS             2233..3933
                     /locus_tag="IYE22_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507000.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(4035..4868)
                     /locus_tag="IYE22_001793"
     CDS             complement(4035..4868)
                     /locus_tag="IYE22_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ9507001.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5172..7337)
                     /locus_tag="IYE22_001794"
     CDS             complement(5172..7337)
                     /locus_tag="IYE22_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9507002.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7597..9891
                     /locus_tag="IYE22_001795"
     CDS             7597..9891
                     /locus_tag="IYE22_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ9507003.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9884..10549
                     /gene="pgmB"
                     /locus_tag="IYE22_001796"
     CDS             9884..10549
                     /gene="pgmB"
                     /locus_tag="IYE22_001796"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ9507004.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10542..11549
                     /locus_tag="IYE22_001797"
     CDS             10542..11549
                     /locus_tag="IYE22_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ9507005.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11571..12590
                     /locus_tag="IYE22_001798"
     CDS             11571..12590
                     /locus_tag="IYE22_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9507006.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         3819 a         2667 c         2268 g         3930 t
ORIGIN      
        1 agattgtaac aactgcatat tcacacagtt aaattagtga cttcaaaatg aaataatgct
       61 cccagcaaaa aattaggctg ggagcattat ttcgttattc tgttcatcaa aaatcagcgt
      121 tttgattcca tttcatcaca agttcctcct catttactgc ttcatctttt tgctgtgttt
      181 gaattgtaaa accttgtgaa agatagaaat gaatagctga ttgatttttt tgatagactg
      241 tcaaagtcag tgttttattg gtttctttta tttttttcat tagttgcgta ccgattcttt
      301 gttgccggta ttcccttttc acaaagattc ctgcgataaa ctgatcaatc accccagcaa
      361 aaccaataat ttcttgatct atttctgcta agtaaatcac tgcatctggt aaagctttcc
      421 taacagattc tacttgattc tcccaataag ttgatggaat gaattcatga gaatccaagt
      481 tagcatgcag ccaaatcgat agaacagatt ccagttcttt ggcagacaat tttgttgttg
      541 tgcggatcat tttttctctc cttacttatt cattgatttt aaaataaaaa ccattttttt
      601 gaagctcttt tacaagtttt cgcccctcaa aaaagtatat cttctcttgt tttacttttc
      661 taataccaaa agaaacagca actttaggat catcagctga aacgattcga taagttccac
      721 caggcaattg atccagcttg actttcccat gatggtcgct ttgtactgaa aacggcacac
      781 catttctagt aagtgttttt ttcttatgtt tcaaaatgaa tgaactacca tttaccggtt
      841 tccctgacga atcactaata ataaatgaaa aatcaagcac ttttttactt aatctatact
      901 gatagattag tcgtccgata aacactaaca gtatgactac agctattatc gttcctgcaa
      961 gaataaacca ttgccgcatg gttctttcct gatttccttt tccttcttct ttttttaccg
     1021 attcgttata aggaatccga tgtcctgtca ctagcaaacg atgcgtgttg atcatatacg
     1081 gcgtacatgt aagcaaggta actaagtctt ctccttctga aatcgttaga acagacgtgt
     1141 catctggcag taccacttgt atatttttaa cttgataagc caatttctcc cccaaaacag
     1201 tcaaaataaa cgtgtctccg ttttctaaac gatctaattc ggtaaataac cgtcgattag
     1261 gtaaaccgct gtgagcagag atcactgaat gcgtcgaatt tccaccaagc gggaaagagg
     1321 ttccttgcaa gacgcctgct ccattttcaa ggatcctacc attgagccga tcataaagtg
     1381 aaatatttaa ttggatctta ggtatattga tacttccgat cagccattcc tctaattggc
     1441 tggatgttgt actttcattt ctgttctttt cattgaaagg atcagtacca ggattgaatc
     1501 cttggtcagt taatcgttgg tttttctcct ccattttctt tcgctgcact tcaatttcag
     1561 ctgcggtccg tttttgtact tgttcgatac gtttttgatc aataaaacta tttaaagaat
     1621 ccacataaaa tggatataaa gcgatcagta accctacgac gatacatatt aataaaaaca
     1681 aacgaaacaa aagtcgacga gactgttttt gcttactctg cttcacatgt gtcctccctt
     1741 gcaagttttt ttgttctttc ttttattata tgggttcagg aaaaaaatag aaaatgaaag
     1801 ccgatgaggt tgtgaaaaag ctgtcctgca tcaagtaata agtacagccc aaaaaaatag
     1861 ctactcatat tttttgttgg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     1921 ttaaggaaga ggttgtgaaa actgcgttat gctttgagta ataagagcgc tagtcagaaa
     1981 atagcttctc atattttcta ggctagtgag cttattaccg agaagcagct gattgaacac
     2041 cgtttaccga gaggtcatga aaaagctact ttgtttcgag tattaagaaa aattctgttt
     2101 caaaacaagg agatgcaaca aaacttatgt cgcatcctct ctccttagaa ctttcactta
     2161 tgtttttctt gtaaaattca taaatatttt catgaatttt ctaatttctt gcctacctct
     2221 cgcataggat atatgatgaa tttatctaaa gtttgcaaag gagttcgaca attgaaaaaa
     2281 tatggcccat ttttagcaat ttttttcatt gctttggcat ttctcatgcc tcatttattc
     2341 acacataaac taattttggg tgctgatgcc ccattccatt acaatcggtt ctatgatgcc
     2401 gcgcaacaaa ttaaatatgg ggatttccat tattttcctt ccctttatgg attccagcaa
     2461 agtggcagga tcgtgaatgc cgtttacggc ccatttttcg cttatttaca aggattgatc
     2521 gtgttgcttg caggtagctg gtttcattat cagattcttt caaatttcat tctttatctt
     2581 atttcaggat tttctatgct gattttactg agatatgtca atgttcgaaa ttgggtaagt
     2641 gtaccagtag ccgttttatt catgagtacg tattctattc aatactggtc tatcaaccaa
     2701 ggattcacta gctgggggac ttgtttttat cctttatgta tgatccctat tgctgatttc
     2761 gtccttcata aaaagttccc tgttatcaaa gtcgctgtct cagtgggact tatgacacag
     2821 atccatttac tgtcttcgat catgttagct ttgatgtata tcccttttta tctttattac
     2881 ttttttaacc agactgaaaa aggaaaagca ctgcttcaac tgattaagtc ggtcttgctt
     2941 tattttgttc tgacagcaaa tatttgggca ggattagcta ttgtttacag tgggaataag
     3001 attcttcctc cctttgttaa cagagaaatg agtcaaaagg cgatagatct ggatggcagt
     3061 tattggctcc attaccctaa ggcctttcct atattgatat tatcaggttt tttgcttttt
     3121 attttctttc aacaaaagaa caccttattc caaaaaatcg tgttgtacac aagtcttgtt
     3181 ttcttttttc tttctactag cttgattcca tggacgacat tgatcgaaaa aaatgtgcct
     3241 tttattagtt tgatccagtt tccatttcga tttttcgtgc catttacgct attgcttcta
     3301 ctttaccttg ccctttcact gaaccagtgg tctgaacaaa agtggttccg tcttttaagt
     3361 atatttggca ttcttatttt ttcagcacag acgatccaga acttatacca gcatctggaa
     3421 aagtgggaaa atgaaacatt tgtcagtcga catacttatc tattcgatac gccggaagaa
     3481 gcaaggaaaa cattcttcag caaagatatg tctaccagct tgttcgtttt ccaaaaaacc
     3541 acacctgact atctgccaat ctataaggag acaaaagaca ataaatacga ccggtataac
     3601 gaagaaatac tagaaaacga aacaactttt ataaaaacac atccaggcgg tcttttgaca
     3661 atcaagtgga aaaacactgg ggacaaaact gttcatatcc ctgttatcgt ctacgatcgg
     3721 actgtgctac agcaaaatgg aaaaacattg actgattatg aagtaacaga tatcgggacc
     3781 ccaatcgtga agcagcaaaa aggaatcaac gagctcacgc ttcattatca aacgccaatc
     3841 tatttctatt tcattctttc tcttactttg attggctggt ttaccttgct atgtttattt
     3901 atttatcata gatacaagct tttacgtgct taggcaaaag ggataaatac atccgatcaa
     3961 aaaactctcc aaacggtata atcgtttgga gagttttatt ttaatcagtt tttttatgtt
     4021 atatgttata ttttttattc gttcacatct aattcgattt ccaccccgta gtgatcactg
     4081 atgattggac tatcgatccc atcaaaaatc cgttgatatt ttttaacctg cattccttca
     4141 gggacgaaaa taaaatctat acgcaatttt tctgtatttt gttcccaacc atctattttc
     4201 ttttccacag tagctgcttt tcctttttca ttcgcgacaa catacgaatc ttgaagaaga
     4261 aggcaacttt ctcttacagt ataataacct tgagagtcta ctggattatt gaaatcacct
     4321 aggaaaaaaa gtggtacgcg agtttccaaa aagtagtttt ccaagttttt ccactcaaaa
     4381 taaaagccac tttttttatc tgtccaccaa gaaaagtggc agctcgagac aaaaatgtcc
     4441 tgatcatcta tctttgtttt cccaatcaag atcttgcgtg tctgataatc ttctttcttt
     4501 tgaaattggg agacggtaag gacctgactt tccaatgttt ttttcgtaag cagtgcattt
     4561 ccttcttcat atttatcata accgatgtgg ctcatttccc aactccaaaa atactctttt
     4621 ccgtgttcct ttaaaagctg aactaaacgg taggcaaaat tatctttacg gataggtact
     4681 tgattcttta ccggacagaa cttcattagc ttcgcaggtt ctacttcttc agattccagt
     4741 aattggttga tttcttgtaa agcaatcact tcataatctt ctaacaggat cttatctgct
     4801 aattcttgca attttttctc tggttctggc tcaagccagc tatgcgtgtt gagtgtcatg
     4861 aatttcattg gttttctcct tgtcttcatt tcgaaacaaa agggttgtga caaaattttt
     4921 gtcacaacct ctccagcaaa tagtgttctc ctgctaactc ctcagtaata agctcaatgg
     4981 accaggagct attttttgtt tggctgtttt tatcacttgg tgcagggcag ctttttcacg
     5041 acctctccac taaacagtat tcaaactgct gcttctcggt aataagccca accaaacgaa
     5101 aaataggagg agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     5161 acaacctctc cttacatttt aatggagccg atcactttac cagctgtcac tgtacctaat
     5221 tgcttgatca ctacttcttc tactttatcg tttgtaaata cgatcatgat cgttggatct
     5281 ttttcttcgt tttggatcac atcaagatcc atctcagcta atttcgttcc tgttcctact
     5341 tcttggcctt ctttgacagc aatctcaaaa gctggctgat tcatttggac tgtatcgatt
     5401 cccatatgga tcaaaacttc gataccatct ttcgtttcca gcccaattgc atgtttgcta
     5461 gggaaaatac ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga tgttggaata
     5521 acagcaaagc cgtcacccat cattttttgt gagaataccg gatcttttac ttcagataaa
     5581 ggaatcactt ctccagaagc tgcggaaact agttcttgtt ctatacctaa gtattgctta
     5641 ctacctgctt gtccatttcc tgtagaaacc atttctggct ctggaatctc tgctcctgat
     5701 tgcagcagat cttcgatatc tgatttcaag acatctgctt ttggtccata aactgcttgg
     5761 actccattgt ctttgacgat caatcccatc gcccctgctc gtttccaggc ttcttcagtc
     5821 cctacttttt ctcgatcatt tacacttacg cgtaaccgag tcatacaagc atcgacatct
     5881 ttgatatttt cttttcctcc cagcaaatga atgatctgga ccacttgctg atctgctgtt
     5941 cctgttccgc tgttagatac aacggtttct tcactgtcat tgtcgtaatt tccgttacgt
     6001 cccggtgtag caaagttgaa ttttttgatc aagaaatttg ctaagaaata cgtaacaaca
     6061 ccaaaagcga tcacacacaa cacgaagttg ataagatctc cacctagacc cgctttgata
     6121 gctaacggtg tccgagtcag caattcgata ttcccaaatg aatggacccg caatggcaag
     6181 atatctgcca tcgcaaatgc agccccttgg atcactgaat aaatgacata taatggcaca
     6241 gcagcaaaca taaacatgaa ctccaatggt tctgttacac ctgttagaaa gacagccaac
     6301 gctgctgaaa aatacattga tttatatctt gctttcttat caggatcgac attacgatac
     6361 atcgcaaaag ccattcccat caagatacct gaagaaccaa tcatttgtcc tactttgaaa
     6421 cgagccggcg tccagttatg caacacaaac tcatattgcg acatatcccc tgcaccttta
     6481 agattcacta aatcagtggc ccaagccaac cataacggat cttgcccaaa tacttgtgtc
     6541 cccgcctgtg cgcctgataa aatctcatac gatccgccaa gctgtgtata gttgatagga
     6601 atcgtcagca tatgatgcaa tccaaatggc aatagtaaac gttctaatgt tccaaataaa
     6661 aacggtgcta gaatcggtgc gctttcttgt gattctgcaa tccataaacc aaaattattg
     6721 attccagcct gtacgtttgg ccacaccaca gcaagaatca atgccacgat tgttgaccat
     6781 aagataacaa caaacggaac aaagcgcttt ccattgaaaa atgacaaagc atccggcaat
     6841 tttctataat tatagtactt attataagcc attgccccga caaatccagc gatgatccct
     6901 acgaatactc ccatattcaa agctggtgct tccaatacac ttgtaaaaaa tcctttaacc
     6961 atgatcttcg taccgaacag agtgtgggta aatgcatctt cattcgcaag catgtcactt
     7021 gtgacaccaa agatagcccc agtgatccga ttgattaaaa taaatgagat cccagctgca
     7081 aatgctccgc ctgctctttc ttttgcccaa cttcctccta ttgctaaagc aaataaaata
     7141 tgaagattac caataattgc ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc
     7201 ggcgtccaat caggattgat caagggaatt gatttcccta tactgatcat caacccagct
     7261 gccggcataa ctgctaccac aaccattaaa gcctttccaa atttttgcca aaactcaaaa
     7321 ctaaatatct ttttcattcc gtttagctcc tttacgcaat cggtttcgtt aaacgtatta
     7381 tagcgtgcgt tttctttttt gtaaatgctt tattttttat ttttctgtct ataccattct
     7441 ataaaaccta taatacgtgt gttcccttct ataattttct cctaaaaatt tattttttta
     7501 agcttttttc tatgaaatat tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat
     7561 tatgaacgta taaaacagct cattaggagg agaaaaatga aacagatcaa acgactattt
     7621 gctattgatc catggaaaat cagcacgaca caattagata aaaaaaatct tcgactgcaa
     7681 gaatctttaa caagtattgg aaacggttat atggggatgc gcgggaactt tgaagaaact
     7741 tactcagggg accaccatca aggtacttat ttggcgggtg tctggtatcc cgataagaca
     7801 cgtgtcggct ggtggaaaaa tggttatcca gaatactttg gaaaagtgat caacgccttg
     7861 aactttatcg cgatagatat ttcgatcaac gatcagctga tcgacttggc ttctttggaa
     7921 ccagaagact tttactggga attagatatg aaaaatggtg tattatctcg tacattcaca
     7981 atcactactt ctactaataa agtacgcatt tccttcgaac gttttctgag cattgtcaaa
     8041 aaagaatcag cttatatcta tttaactata gaaatgcttg aaggtagcgg aacagtcaaa
     8101 ctcgtatcaa aattggatgg aaatgttcaa aacgaagaca gcaactatga tgaacatttt
     8161 tggaatgaga ttgatcgtgg aactatagaa gaaatcggct atttgacgac taaaactatt
     8221 ccaaacaact ttgggattga acaatttact gttactgcag caatgcgcca tttcgttaat
     8281 ggaaaatcag tcatccctga ctatcacgaa gatactttag cattacatgg ttgcttgtct
     8341 gcagaattat ctcaaggtga gacatggtca gtggaaaaag aaattatcgt tcttactagt
     8401 agagatattc cagaagcgca gcaagcagaa aaagctgtct cagaattaac gaaccagaca
     8461 aacaattata tccaagcaaa agaagagcaa acagcggctt gggcagaacg atggaagctt
     8521 gccgatgtgg tcatcgaagg agacgaagaa gcacaacaag gtattcgatt caatttgttc
     8581 caactattct ctacttacta cggcgaagat gaacgattga atatcggccc caaaggattc
     8641 acaggagaaa aatacggcgg agccacttac tgggatacag aagcgtatgc tgtccctctt
     8701 tatcttgctt tagccaaacc tgaagtaact aagaatctgc tgaaatatcg gcataatcaa
     8761 ctgccgcaag caatccacaa cgctcagcaa caaggattaa aaggcgcgct ttatcctatg
     8821 gtcaccttca ctggtgtcga atgccataac gagtgggaaa tcacattcga ggagattcac
     8881 cgaaatggcg cgattgccta tgcaatctac aattatacga attacaccgg cgatacggaa
     8941 tatttgaaaa atgaaggact ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt
     9001 gtccattttt caaaacgagc aaataaatac atgatccacg gtgtgaccgg cccaaacgaa
     9061 tatgagaaca atatcaataa caattggtat acaaatacga tcgcagcttg ggtcttgcgt
     9121 tacacgcgag aaaattattt aacttatcaa aaagaagctg ctatcactat cagcgaagaa
     9181 gaactagcca aatggaaaga tatcgcagag aacatgtact tcccagttga cgaagaatta
     9241 ggaatctttg tccagcatga cacattctta gataaagatc tcatgcctgt atccgatatt
     9301 cccacaagcg atcttcctct gaatcagcat tggtcttggg acaaaatcct tagatcttgc
     9361 ttcatcaagc aggcagatgt actacagggg atttatttct tcaatgatac ttttacaaaa
     9421 gaagaaaagc aaaaaaactt tgatttctat gaaccaatga ctgtccatga gtcttccctt
     9481 tcgccaagta tccattcgat tttagcagca gaattaggaa tggaaagcaa agctgtagaa
     9541 atgtaccagc gaacagctcg cttggatttg gataattaca ataacgacac agaagacggc
     9601 ttgcacatca cttccatgac tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg
     9661 aaaactgccc atgatcagtt gagattcgca ccatttctgc caaaaacttg gaaagcttat
     9721 tctttccata tcaattatcg cggacgttta ttgttcatct cagtcacaga tgaacaagtt
     9781 actttcgagt tattagaagg ttctcctcta gaaatttacg tctatgaaca gtcttattct
     9841 ttagcggatc gtctagaaat aacgctaaga aaggaggaag caaatgttta aaggggtatt
     9901 gtttgattta gacggtgtca ttaccgatac tgctgaattc cattatcgtg cttggcgaaa
     9961 gcttggtcag gaaatcggca ttacgatcga ccacacattc aacgaacagc taaaaggcgt
    10021 cagccgagaa gactcactct ctttgctttt agcttatggc ggaaaagaac actctttttc
    10081 aaaggaagaa tttgcagaat tagcgaaaag aaaaaacgat tattatttag agatgatcca
    10141 gacgatcgaa ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga
    10201 agaaaaaatc aagattgctc ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa
    10261 aatgggtctt acaccttact ttgatgctat tgccgacccg gcagaagcaa caaacggaaa
    10321 acctgcgcca gatatcttcc tccttgcagc aaaagctgtc ggactaaaag cagaagaatg
    10381 tatcgggatc gaagacgcgc aagccggtat ccaagccatc ctctcaagtg gcgctcaacc
    10441 aattggcgtg ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc ctgatacgac
    10501 ttttcttacg atcgattact tgaaagaaaa gtggcatgac catggataat ccagagatct
    10561 ccgtcgtacc agttccagag actgaaataa agaaaatcac attgaagaac gactacttag
    10621 aagtcgttct tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag
    10681 gaaaatcaga aaatgtttta ttgtcttacg atcgtttcga agatgttcta acagacaaaa
    10741 gcttttttgg agcaactgtc ggacctgtag ccggaaggat ccgggatgct tcttggggtg
    10801 agcatagatt ggagaaaaac tgcggatcac accatatcca cggtggcacg aaaggctggt
    10861 ctttccaatt ttgggaagta gaagttttca aaactccatc atcaataggt gtggtctttt
    10921 acttaaaaga tgaattttct acctatccag gaccaatcac tgctacgatc acttaccgtt
    10981 taacaaaaaa cgaattagag atgacaacct ctgctagtag ccttgttgaa accatctgca
    11041 atccgaccaa ccatgcctac ttcaaccttt caggaaatgg aacaagagac atctatgaac
    11101 atcagttaac ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg
    11161 gaaattggac aaaaaaagaa aaactgccaa tcgactttag aaaatcccca acactgcaag
    11221 agatccttgc atgttatcca gatggactag atgatgtatt tcttttgcat catcctaggc
    11281 tttccaaaac ttccttacaa ttattcgaga agcattctgg tcgccaaatg actatagcaa
    11341 cttctaataa aagtatggta ttattttcta cgactggatt cgaagctgat ttctctgtca
    11401 atggaaaaca gatgcattca aactacggat tagcaatcga accgcaagaa atccctgata
    11461 tcgttcattt ccccaaattt ggatcgatca acctccatcc tggtcaagaa cggatcagtc
    11521 agacgattta tcggttctct gcacaataac aagagaaagg tgtttttatc atggctatta
    11581 ccgtaaaaga tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct cgtgtcatca
    11641 acgatcatcc tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt atggatgaac
    11701 taggttatgt tcctaacatg actgcccgca atctaggaaa acgcatttcc agtgccatcg
    11761 gcgtaatctt accccctctt gattcaaaag agcggatcgg taacccattt tatcttgaga
    11821 tcatggaagc aatcaacgaa gaagcgcgga attatgatat gactacagca atcgctactg
    11881 ctaaaaattt tgaattgctg ttagagaacg tccagcgtat gcatttacaa aaacaagtag
    11941 atggttttat tctagtgtat tcagatagtc aagatactgt cattgattat ttataccaaa
    12001 accacatccc tttcactttg atcggacagc cttatcatca tgagaacgaa atcgtttatg
    12061 tagataatga taaccagctt ctaggtaaac aagcaacaga atttttgatc cagaatggac
    12121 ataagaatat cctgtttgtg acaaacacga ctcatgaaag tttgtatttc gaacgatatt
    12181 tcggttatca aaaagcaatg atgatggctg gtcttcctgc gttatccagt gtagtactag
    12241 agcatccgga ggattatctg tcctttgaga aaacgttgaa agaaaacgaa gccacagcat
    12301 tggtggtcat tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa atgtatggct
    12361 atgtcattcc agagacactc tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa
    12421 cacaccctta ccttacaagt atcgatatcg atatctccga attaggacgt gtcgctatgc
    12481 aaaagctgcg cgaattgatc catgaagaat tgagcagtgg cgtacgtttg gtcatccctc
    12541 atcgcttgat caaaagagag acagtcgttc ctttagaaaa cccatactaa atagctgact
    12601 cactaaatac gctgaccatt tatcctatgg tcagcttttt atgtaaaaga agatactact
    12661 tctaacacat tgtttatctg taaa
//
LOCUS       DACIRY010000075        12680 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.076, whole genome shotgun sequence.
ACCESSION   DACIRY010000075 DACIRY010000000
VERSION     DACIRY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.076"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(218..1015)
                     /locus_tag="IYE22_001799"
     CDS             complement(218..1015)
                     /locus_tag="IYE22_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ9507007.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IYE22_001800"
     CDS             complement(1012..2196)
                     /locus_tag="IYE22_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ9507008.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IYE22_001801"
     CDS             complement(2221..3468)
                     /locus_tag="IYE22_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ9507009.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IYE22_001802"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IYE22_001802"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ9507010.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IYE22_001803"
     CDS             5448..5762
                     /locus_tag="IYE22_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ9507011.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IYE22_001804"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IYE22_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ9507012.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IYE22_001805"
     CDS             6447..7091
                     /locus_tag="IYE22_001805"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ9507013.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IYE22_001806"
     CDS             7106..7435
                     /locus_tag="IYE22_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507014.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IYE22_001807"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IYE22_001807"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ9507015.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IYE22_001808"
     CDS             8423..9247
                     /locus_tag="IYE22_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ9507016.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IYE22_001809"
     CDS             9240..9587
                     /locus_tag="IYE22_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ9507017.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IYE22_001810"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IYE22_001810"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ9507018.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IYE22_001811"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IYE22_001811"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ9507019.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IYE22_001812"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IYE22_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ9507020.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
BASE COUNT         4036 a         2375 c         2719 g         3550 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt
//
LOCUS       DACIRY010000076        12670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.077, whole genome shotgun sequence.
ACCESSION   DACIRY010000076 DACIRY010000000
VERSION     DACIRY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.077"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            6..305
                     /locus_tag="IYE22_001813"
     CDS             6..305
                     /locus_tag="IYE22_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9507021.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IYE22_001814"
     CDS             378..1739
                     /locus_tag="IYE22_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ9507022.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IYE22_001815"
     CDS             1761..2021
                     /locus_tag="IYE22_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507023.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IYE22_001816"
     CDS             2035..2787
                     /locus_tag="IYE22_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ9507024.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IYE22_001817"
     CDS             complement(2881..3486)
                     /locus_tag="IYE22_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9507025.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IYE22_001818"
     CDS             complement(3539..4513)
                     /locus_tag="IYE22_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ9507026.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IYE22_001819"
     CDS             complement(4891..5670)
                     /locus_tag="IYE22_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9507027.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IYE22_001820"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IYE22_001820"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ9507028.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IYE22_001821"
     CDS             7161..8558
                     /locus_tag="IYE22_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9507029.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IYE22_001822"
     CDS             8639..9079
                     /locus_tag="IYE22_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ9507030.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IYE22_001823"
     CDS             9227..9553
                     /locus_tag="IYE22_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9507031.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IYE22_001824"
     CDS             9554..10303
                     /locus_tag="IYE22_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507032.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IYE22_001825"
     CDS             complement(10383..10838)
                     /locus_tag="IYE22_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ9507033.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IYE22_001826"
     CDS             complement(11265..11540)
                     /locus_tag="IYE22_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507034.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IYE22_001827"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IYE22_001827"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ9507035.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACIRY010000077        12444 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.078, whole genome shotgun sequence.
ACCESSION   DACIRY010000077 DACIRY010000000
VERSION     DACIRY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.078"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(315..398)
                     /locus_tag="IYE22_001828"
     tRNA            complement(315..398)
                     /locus_tag="IYE22_001828"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="IYE22_001829"
     CDS             926..1426
                     /locus_tag="IYE22_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507036.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="IYE22_001830"
     CDS             complement(1616..1960)
                     /locus_tag="IYE22_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ9507037.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="IYE22_001831"
     CDS             complement(2114..3034)
                     /locus_tag="IYE22_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9507038.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="IYE22_001832"
     CDS             3221..3712
                     /locus_tag="IYE22_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ9507039.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="IYE22_001833"
     CDS             complement(3736..6111)
                     /locus_tag="IYE22_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ9507040.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="IYE22_001834"
     CDS             6437..7063
                     /locus_tag="IYE22_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ9507041.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="IYE22_001835"
     CDS             7086..7790
                     /locus_tag="IYE22_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ9507042.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="IYE22_001836"
     CDS             7982..8815
                     /locus_tag="IYE22_001836"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ9507043.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9012..10940
                     /locus_tag="IYE22_001837"
     CDS             9012..10940
                     /locus_tag="IYE22_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ9507044.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11078..11533
                     /locus_tag="IYE22_001838"
     CDS             11078..11533
                     /locus_tag="IYE22_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ9507045.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12003..12272
                     /locus_tag="IYE22_001839"
     CDS             12003..12272
                     /locus_tag="IYE22_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507046.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         3800 a         2318 c         2249 g         4077 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atgggtgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctcttatt tgagaaatgt tctggttttt tattttcttt aataataatc
     8881 attataaata ataaaaacaa catcaagctt aatcaccaaa tgataaccat tatcgttctc
     8941 atttagctgt tattattatc aattacattt ttattatgct atccttgttt tgacaaggag
     9001 gtctaataac gatgactaat cagaaaaaag cttttgcagc aacaatcggc tgccttttat
     9061 ttatgatctt cggttttatc ttcgaccgat tctctcttac catcacccca cttttttata
     9121 tcagtgccat tatcatcggt ggatataaac aaacatcgga aggcttgaaa gaattgtgga
     9181 atgatcggac actgaatgtg gatttattga tggctttagc tgctattggt gcctgtttga
     9241 tcggtaatta ctttgaagga gcaatgttga cattcatctt ttgtttaagc ggtgcattag
     9301 aggaatacac aactaataaa agtcaaaaag aaattactgc tttgatgaat cttcaaccac
     9361 aaaaagccca attattaaaa gaaaatggac aaatgcaaga agtagatgtt tctcaactaa
     9421 aggtggataa cttggtattt gtcgctaaag gagcagctgt tccgatagat ggctttttag
     9481 aaagcacaca ggcaacgatc gatgagtctg cattaagcgg tgaatctgtg cctgtagaaa
     9541 aatatttagg cgatgagatg tatgcaggaa cgctgaatca aggaaatccc atcacgattc
     9601 gagtaacaaa aacgagttcc gaaactgtct ttgctaagat tatccaatta gtagaagaag
     9661 cgcagaatac gccaactcaa acagcttcat ttatcgaacg aatcgaaaat aattatgtaa
     9721 aattgatttt gcttgctgtt cctctaatga tccttctccc ccactttttc ctaggttgga
     9781 gttgggatga aagcttttat cgaggaatgg tattattagt tgtggcttct ccttgtgcgc
     9841 ttgtcgcatc tgctacacca gcttctctag ctgctctttc aaattctgca aaaaatggga
     9901 ttttaatcaa aggcggtatc catttagaaa agctttctga attaaaagcg attgcctttg
     9961 ataaaacagg aacgctcact aaaggaaaac cagttgtaac tgacagcttt ttctttgatg
    10021 aaaaggattt ggcacaacaa cttcttgttg ctatggaaca aaaaacgacg catccacttg
    10081 ctcatgcgat tttgcaatat ttcgattgca caatacctga agaaatccaa tacttaccaa
    10141 tagaagaaat tactggtttt ggattacaaa ctacctattt aggtgcgcaa tggaaagttg
    10201 gcaagcacgc atatgatcct gagacgatga taatcagcaa agagattgcg gaaatgatcg
    10261 aacgtttgga aaatcaaggc aaaaccgtga tttatctttc taaagatcag cagttaattg
    10321 ctgttttagg tctgctagat attccaaaag caaataccca acaagtaatt tcctacttta
    10381 aatcccaaaa catccataca agtatgataa ccggcgacca ttctggcaca gcaaaggcca
    10441 ttgctgaaca agtagggatt gaacattact atgcaagctg tactccggaa gaaaaaacac
    10501 aactagtcaa aaaagagaat gaactttacc aagtaaatgc gatggttggt gatggagtca
    10561 atgatgcgcc tgctttagca gccgcttctt taggtgtcgc tatggggcaa gggacagatg
    10621 ccgctatgga tatcgcagac atcgtcttaa tgaaaagcga cttaaataaa tttgtctaca
    10681 gtcatcggct atctttaaaa atgcggcgga tcatcaaaca aaatattatt ttttcgattc
    10741 ttgttattct cttgttgatt gcttctaact tcctgcaatt tttaaatcta cctcttggag
    10801 ttgtaggaca cgaaggaagc acaatattgg ttattctgaa tggcttgcgt ttactaagac
    10861 ctttacctga tctttctgat tcttcaaaaa aatgtaagtc ttgtccacta tacaaagcgc
    10921 ggctgtcttc tgtcaaataa gcaaagtgtt tttttatgat tttgtttatt ttatgaaaaa
    10981 aacagcttat gaataggtct tgaaggatga attcctactt ttgtaacttt ctaaaaacat
    11041 gttatgattg tttcgaagaa agtgaggaat atatttcatg gaaagttggc tttttttagg
    11101 aattatttta cttgtagcaa ttttagctaa aaatcaaagt ctgatgatag cggcagcttt
    11161 tgttttgatt atgaagctca taccagcctc ccgttccttt ttcccttggc ttcaaggcaa
    11221 aggaatcaat attggggtga cgatcatcac agcggctatt ctaatcccta tcgcaacagg
    11281 agaaatcggt ctacgtgatc tattagaagc ctttaaatcc cctattggct gggtagctgt
    11341 tttttgcggt gggctagtgg ctgtcttatc ttccaagggt gtaggattga ttgcttctga
    11401 tccacaaatc actgttgcac ttgttttcgg cacgatttta ggtgttgtct tcttaagagg
    11461 aatcgccgcc ggaccggtca ttgcttctgg tatcacttac tgtatcttac aagtatttgc
    11521 tttaggaaaa tagaacaaat tttgtctatt ttaggagatt cttaacaccc ccaatttact
    11581 gatccgtgat atactgacaa cgaggtgata caatgttcga aaaattaaag tatttcatag
    11641 gaacaatcgg ctggtttctt ctagcggtac ttttgattct gggcttagct tatctattat
    11701 ttcctgattt gatacaaaat gtgtttgata ctgctttttg attgacctat taaaacgata
    11761 taggggatgc tcctgcgatg caggggcttt ttttaatcaa tgttttgtaa taatttaggc
    11821 cgtgccaatg agacttccta tagaattcga tgctcttttt tctttttggc tcttctgctc
    11881 ttttttgtta tactagatta cagtggacaa aagaaagaat gatcgcatcc acttcaacga
    11941 atatcaaaat ggaagaatta gtgcctgttt tcaggttact ttgaaaagaa aggaagacgc
    12001 ttgtggaaac ttttgaaggt cttttaattg atacgtacgt tttacaagaa cagccaatga
    12061 tgctgactgg gatcaacttg cagcttgagg accgtgtgat caatggcctg cttaaagatc
    12121 gctatttggc aaaatatatc ttacgtttgc ccctaaataa atatgtgctg gaagtaagtg
    12181 gcaaatggaa ccgtcggaac cagttggtca taaaaaaaat gttgataaaa aatatcgatg
    12241 aatacattaa aataatcggt acaccagaat agcctttact gatcaaagga tactggttat
    12301 aaaataactg cagtttctta atagaaaaac aaagaacaac agggaatcgt atcccttttc
    12361 aaaaaaaact ttgcaaacgg ttctctttgt tatataataa cttagtacca aacattccca
    12421 ttcaaacttt gtttaaatag ataa
//
LOCUS       DACIRY010000078        12327 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.079, whole genome shotgun sequence.
ACCESSION   DACIRY010000078 DACIRY010000000
VERSION     DACIRY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.079"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(386..1693)
                     /locus_tag="IYE22_001840"
     CDS             complement(386..1693)
                     /locus_tag="IYE22_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ9507047.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1696..2277)
                     /locus_tag="IYE22_001841"
     CDS             complement(1696..2277)
                     /locus_tag="IYE22_001841"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ9507048.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2393..2490)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2849..4141)
                     /locus_tag="IYE22_001842"
     CDS             complement(2849..4141)
                     /locus_tag="IYE22_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9507049.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(4138..4761)
                     /locus_tag="IYE22_001843"
     CDS             complement(4138..4761)
                     /locus_tag="IYE22_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507050.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4764..5600)
                     /locus_tag="IYE22_001844"
     CDS             complement(4764..5600)
                     /locus_tag="IYE22_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9507051.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5617..6504)
                     /locus_tag="IYE22_001845"
     CDS             complement(5617..6504)
                     /locus_tag="IYE22_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9507052.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6520..7803)
                     /locus_tag="IYE22_001846"
     CDS             complement(6520..7803)
                     /locus_tag="IYE22_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9507053.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8486..9169)
                     /locus_tag="IYE22_001847"
     CDS             complement(8486..9169)
                     /locus_tag="IYE22_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ9507054.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(9187..9804)
                     /locus_tag="IYE22_001848"
     CDS             complement(9187..9804)
                     /locus_tag="IYE22_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ9507055.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9814..10638)
                     /locus_tag="IYE22_001849"
     CDS             complement(9814..10638)
                     /locus_tag="IYE22_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ9507056.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10631..11191)
                     /locus_tag="IYE22_001850"
     CDS             complement(10631..11191)
                     /locus_tag="IYE22_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507057.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11365..12321
                     /locus_tag="IYE22_001851"
     CDS             11365..12321
                     /locus_tag="IYE22_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9507058.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         3715 a         2696 c         2090 g         3826 t
ORIGIN      
        1 attttaacaa tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat
       61 tttattgttc gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt
      121 cctttttatg gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca
      181 acaaacatga gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc
      241 tcacgacctc ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa
      301 cggcaaaata cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg
      361 atttcacaac ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat
      421 cttttttcaa tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga
      481 tgccatttcc agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa
      541 cattgaaacc cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc
      601 tgaaatcaac ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta
      661 acattcctcc accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga
      721 ataatcctaa aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc
      781 ccgaaagttg aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa
      841 taccgcctaa gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc
      901 caacattttt cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc
      961 tgaccatgga aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa
     1021 aataaaaggg ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca
     1081 ttcccatgaa agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac
     1141 gaatgaaccc ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa
     1201 gtagcagatt tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct
     1261 cgattgctac aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag
     1321 gagggaatag ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag
     1381 aagcgataat agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg
     1441 gagctactgc ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt
     1501 taactgtcaa ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat
     1561 aagtcatctg ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg
     1621 ctcctgcata catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg
     1681 tttctttttc cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga
     1741 agcaatacgc gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc
     1801 ttggaaagac ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca
     1861 taattcgatc aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac
     1921 tttgtcttct gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt
     1981 aaaagaataa acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct
     2041 agcgaagatc attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga
     2101 agcttcaatg gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt
     2161 tcccatttgt tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa
     2221 tacgccctcg cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt
     2281 atttgctcct ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat
     2341 gtttctgtta aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca
     2401 atcttttact tatagtccag catttacggt actggctaga aactgtcatc catattatga
     2461 cgatatataa gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt
     2521 ttgcacgtga aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac
     2581 agtctaacac tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa
     2641 tgatttttaa tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct
     2701 atctaaaaaa gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt
     2761 ttccgaataa atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact
     2821 acgggaagta atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca
     2881 ctactgcttt tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa
     2941 taccatctac gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata
     3001 gatcatctaa taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta
     3061 cctcatatgg ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt
     3121 catccttttt atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc
     3181 gtcctttgct gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg
     3241 aatacgtcaa aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag
     3301 agatatcatc ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta
     3361 aatacatttc tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag
     3421 ctagttcgct tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat
     3481 gcatcccata aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc
     3541 aaaaattcac tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt
     3601 gcttatttcg atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt
     3661 cgaagtggat agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg
     3721 tgtgatacgg tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt
     3781 ggaccgttat tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa
     3841 catcatatcc ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg
     3901 taataatgac ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac
     3961 atgtctttac ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc
     4021 ttgtttgaga tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa
     4081 ttgcctcata aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca
     4141 tttaaagtca ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata
     4201 cagtaatgca gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca
     4261 taaataccac agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa
     4321 ataaacaaga ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact
     4381 atgggataca aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt
     4441 tcccactaat aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat
     4501 gatgaaacac atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta
     4561 atggacagat agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg
     4621 agcagacagc actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt
     4681 actgataaga ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat
     4741 ctcttctatt ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca
     4801 agatatttat tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc
     4861 gcaaacatac tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc
     4921 gataccgtgt aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat
     4981 ttccaataaa actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata
     5041 attttggtaa agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct
     5101 ttggggatgc tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa
     5161 tgtggtaaaa tcagcggcac atacgtccca accaaattca gccgattgta aataataaac
     5221 tgcggaatca aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag
     5281 aatctttttc ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga
     5341 actgtcccta tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat
     5401 gaaattccag caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga
     5461 atcaaattca gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc
     5521 cataagaccg gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat
     5581 ttcgttactg tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga
     5641 tgatttgaaa atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc
     5701 actagcatac cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa
     5761 gttcgtggca tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa
     5821 cgccgcgatt gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt
     5881 gatcttgtaa aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga
     5941 gcctgggatc tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc
     6001 agacatcaaa acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg
     6061 tgcgcctaat cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca
     6121 aactagagcg atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa
     6181 gaaagtcgtt cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag
     6241 tttcacaggt acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg
     6301 ggcatcactc agcatcttta tataattatc caagccgatc catgagactg gatttcccaa
     6361 acgataatcg gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag
     6421 aaatcctagg atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa
     6481 ccgttttagt ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc
     6541 aaactcataa agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc
     6601 aactaaaaga ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac
     6661 ttcgccgttg tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc
     6721 atcatctgca cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc
     6781 ttttaaaatt tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc
     6841 ttctttatgg gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc
     6901 agattctcca tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg
     6961 agcccctgac aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct
     7021 atccctaacc gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg
     7081 atgggcccct ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg
     7141 attttcaaat cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc
     7201 aacttgtggt atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata
     7261 gattcctaat tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt
     7321 tgaagctggt tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa
     7381 cgaattcacc ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata
     7441 ggattcatct gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc
     7501 aatctgattg atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc
     7561 tgatgctgct agtgtaccta attttgtgta atatccttca taatcataaa attcataatc
     7621 gatcgtcaca tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg
     7681 tcggacttgc gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc
     7741 acaagccgtc aagaagaata acataacact taaagctaag atgattctca ttcttttctt
     7801 cattctctgc acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac
     7861 atatttatta aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg
     7921 cctaggatga acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat
     7981 cgctaaaaac acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat
     8041 cagacaactg cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct
     8101 tccctaaaaa acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt
     8161 cgatgcacaa gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc
     8221 ccgacaattt agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca
     8281 ttctatagaa agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca
     8341 aaaggtttta actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt
     8401 tttgaattct gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc
     8461 ttcctcgtct tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt
     8521 tttccatttt acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg
     8581 ctcctgcttg aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag
     8641 gaagttcagg atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac
     8701 tgatattgat aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg
     8761 ttaccgtact gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag
     8821 gcgtaggacc gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt
     8881 aggaagaacg agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag
     8941 ctgcctcaat aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta
     9001 ctgcattcgc atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga
     9061 tcaattttcc aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga
     9121 tatcttttct caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct
     9181 cctttgctat ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt
     9241 ttgcgcataa ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg
     9301 atagttggtg gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac
     9361 gaacagttgt gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc
     9421 ttggatagtt atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg
     9481 atccccataa gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc
     9541 ccaatttgat tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc
     9601 ttgtagttct tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc
     9661 attttcttgc tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc
     9721 taagagtatc actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg
     9781 ctttttccac ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata
     9841 ttcttctagc gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg
     9901 taagtgccaa ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac
     9961 gataaatcca tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta
    10021 acttgcatcc aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac
    10081 tgccagacct gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt
    10141 tgtcgcctcc tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa
    10201 aacttgttgt tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc
    10261 tgcttcagca gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta
    10321 tccgttactt aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc
    10381 tggaccaacc aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt
    10441 acgatctttt gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt
    10501 actagcagtt gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga
    10561 atcatcatcg ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa
    10621 tcctaaaaat ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct
    10681 catgaacgta agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc
    10741 cttcgatctg tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc
    10801 attcatcata ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt
    10861 tcaaagggat cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg
    10921 ttttgatttg cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt
    10981 gacgcaaaat cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct
    11041 gcatcgcttc agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac
    11101 tgatcagcaa gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac
    11161 tcataataag atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact
    11221 taagttttga tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag
    11281 tcctctcctc tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat
    11341 ccaactatga aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt
    11401 accatcgcac atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt
    11461 gcttccagaa cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct
    11521 tacggaagct atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta
    11581 ccaaatagac agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta
    11641 tgtgaaaaag ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa
    11701 gaaaagaatt tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag
    11761 ttacgctcaa taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc
    11821 ggcagttaca aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga
    11881 gctctgttgg atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa
    11941 cctgaagtag ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc
    12001 actatcttgc gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa
    12061 atgccaaaag tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg
    12121 cgtgctgatc gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg
    12181 aatacatctg aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac
    12241 ttgcccaatc gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa
    12301 gttctatgga aaaatatgta attttaa
//
LOCUS       DACIRY010000079        12178 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.080, whole genome shotgun sequence.
ACCESSION   DACIRY010000079 DACIRY010000000
VERSION     DACIRY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.080"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            122..1153
                     /locus_tag="IYE22_001852"
     CDS             122..1153
                     /locus_tag="IYE22_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ9507059.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            1150..1917
                     /locus_tag="IYE22_001853"
     CDS             1150..1917
                     /locus_tag="IYE22_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ9507060.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            2017..3585
                     /gene="liaX"
                     /locus_tag="IYE22_001854"
     CDS             2017..3585
                     /gene="liaX"
                     /locus_tag="IYE22_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ9507061.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3885..4202
                     /locus_tag="IYE22_001855"
     CDS             3885..4202
                     /locus_tag="IYE22_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ9507062.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            4202..4552
                     /locus_tag="IYE22_001856"
     CDS             4202..4552
                     /locus_tag="IYE22_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ9507063.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4719..5657
                     /locus_tag="IYE22_001857"
     CDS             4719..5657
                     /locus_tag="IYE22_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ9507064.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5672..6502
                     /locus_tag="IYE22_001858"
     CDS             5672..6502
                     /locus_tag="IYE22_001858"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ9507065.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6546..7571
                     /locus_tag="IYE22_001859"
     CDS             6546..7571
                     /locus_tag="IYE22_001859"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ9507066.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7586..8524
                     /gene="galU"
                     /locus_tag="IYE22_001860"
     CDS             7586..8524
                     /gene="galU"
                     /locus_tag="IYE22_001860"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ9507067.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            8649..9089
                     /locus_tag="IYE22_001861"
     CDS             8649..9089
                     /locus_tag="IYE22_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ9507068.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            9092..9694
                     /locus_tag="IYE22_001862"
     CDS             9092..9694
                     /locus_tag="IYE22_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ9507069.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9753..10859)
                     /locus_tag="IYE22_001863"
     CDS             complement(9753..10859)
                     /locus_tag="IYE22_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ9507070.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            11069..12070
                     /gene="ccpA"
                     /locus_tag="IYE22_001864"
     CDS             11069..12070
                     /gene="ccpA"
                     /locus_tag="IYE22_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ9507071.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         4039 a         2101 c         2574 g         3464 t
ORIGIN      
        1 tttttcattt ggctggactt attaccgcag atgcaagctt ttgaacactg tttattcggg
       61 aaagggtttg tgacaaaata tgtcacaaac ccttttcatc atttcataga aaggataaag
      121 aatgcaaaaa aaaattagca tcatcactat attttttatt tgtgtgctta cttcatgggt
      181 tattcagcat caagctgctt tatatgaagt acctgtcatc gagatcgaaa aagtacgaat
      241 aaaagaggat catcaattga ttacaggaag actgatgaac acgaatcaaa aaggagaatc
      301 gatttattta tcggttcctt atcaaaaaaa tcaaatagaa acagcaaata taaaagcgag
      361 acaaatgtat ctagtccaaa tgcatggaga tgaatggcag ctagtagcaa aaaaaaatga
      421 tggctggctt ttcttcttca caagtatctt cattggcgcc atgttgctta ttggcggaaa
      481 acatggtcta tttgctttag gaagtctcat catcaatatc gttttacttt tacttttgtt
      541 gctcatcttt actaaaatag aagggatttc tctattaaat atatctatcc tgttcgtcgt
      601 tatagggatg attattagca tattggcttt agatggatgg aatagaagca gtataatcaa
      661 aatttcagct gcagttacga gtgttttttt agcatttttt atttgtcttt tgacaatgaa
      721 tctttggaaa gaccaaggac tgcgctatga ggaactgaat tatctgacac gaccgtatcg
      781 ttctgttttt ctttctagcg tactgattgg aacagctgga ggaacactcg ataccgttat
      841 cacagtcatt gcgacattag aagaattgac taagcgacaa ccagaaataa cagcgaaaca
      901 gttatggctt tcgggaaggc aagtgggtcg agatgtgatt ggctcgatga ccaatatcct
      961 tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt ttatatttag gaaatcaatg
     1021 gtcgttcaaa gaaacgatag aacagcattt atcattggaa tttttacgcg tcattgctgg
     1081 cagcttagga atagtattat cgatccctat ctcggtcctc ttttttctga ctgtaaggag
     1141 gctgaaacga tgaatgtact tattatactt gttgggattt tagcaatcag cgtgctactt
     1201 attggcggta cgcaaggtat gtatattctt ttaggcttat tcatcaattt agggatcttt
     1261 ttcttgttgt tattcggtta tcatcagaag tggccgatac tggcattgtc ggtcatagga
     1321 tttttattga ttgctgtggt tatcttattt tttatcaacg gttataatct aaaaatgcgt
     1381 gcagcttttg tgagtatact gatcttttta ttttgtttcc tactattgct tcctatcact
     1441 gattttttag caatccaagg ttttacaacg atagaactag aagaattatc tggcttagat
     1501 aagactttag cgattgattt tagattactt acgagatcgt tgcttctgat tagtcttagt
     1561 ggtgctgttt tagatgcgag tgtagcaatt tctagtggga cattcgaagt atatcaggca
     1621 aatccgcaat tatcttttta ccaattgcgc cattctagtt tcgctattgc taaaaaaata
     1681 ttggcgtcaa cagtaatgac acttcttttt gcattcatgg gaagttcttt agcgttgatc
     1741 ctctggttta ttgatttaga cattcctttt cagcaagtga tcaatgaaaa atcatttgtt
     1801 ctagaatata cgatggctat tcttaccaca ttgtccgcac tacttgttct tcctttaacc
     1861 tgcgtgacta cagcatatct ctttacaaaa aagaaagaac aactcaagat ggagtaaaag
     1921 ttacaactta agtcctatga catttttact gaatcagtgc ataatatgga tgtaaggaaa
     1981 agcttgcaaa caattttgct taaggaggca atttccatga aagaaagaga acgtatttta
     2041 gatttagtga aaaaaggaat cctttcgact gaagaaggct tagatttatt agaaagcatg
     2101 gcaactgaaa aagacgaaaa gcaaatcaaa aaagaagcag acaaggtaac tgctagtcat
     2161 aaagaaaaag atcaggcaag tcaattgatc gataaacttg aaaacggcga agaagaaatt
     2221 tctgagccag ttgatccaaa ggaaagagaa agacaagatc aagaaaactt agaaaagatc
     2281 ttagatgaac tagcaactga agcgaataaa acttcggcgc gtctagatga agtaaatgct
     2341 caattcgctg acaataaggc agcacgcaat gagaaacagg aagcattgat gcagctgaat
     2401 acaaaagaag aattaggcga gttgacagaa gaagaattag cacagcgtca gacgttagaa
     2461 gcagaaatca aagagttaga agctagtggc gatacactgt tagaagaaca attgaaatta
     2521 gaagcagaat taaaagatat ccgtaagaat caatggtctg agaaaaaaga aacgtttact
     2581 gataagtttg aattgccaga tgattggaaa gatcaagcaa cagatacact gaatcaagta
     2641 ggcgaaaaaa tgtcagaagc tggatcacag ttaggaaaat tcttgaagaa aacattccaa
     2701 actgtttcag aaacagtgaa tgacaacatg gaatggaaag atgtcagctt gcgtgttcct
     2761 gggattgcga caacgaaatt tgagcatgaa ttttactatg aagctcctgc agcaagtatc
     2821 cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag caacgatgtg
     2881 aaagttgaag ctaagatcaa actttacgga aaaatgggtg ctgaaccatt tgaagcattc
     2941 tctgaacgga gccagattga ggttaatgaa gatcatatct ccttccaaat tccaaataaa
     3001 cgtgtgcgtg cagaccttgt tttctactta cctaaacgag tatacgacca cgcagctatt
     3061 aaacttctaa atggcaacat catgattgaa acattggaag ctaaagatat ttatacaaaa
     3121 tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa cgatgctgga agtagaaggt
     3181 gtaaacggca atatcgatat tcgaaacggg aacatcttag attctattat cgaaacagta
     3241 aatggtacag tgacatttgg tgcgacagca gaaaaccttt ctgtgtcttt ggtaaatggt
     3301 gatgtacgtc ttacgattaa agaagataat ttgaaaaaag tagaagcaag ttcagtgaac
     3361 ggaaatgtca aagtcgcttt accagatggc atcggtttgg aaggacatgc taaaacaagt
     3421 cttggaagta tcaacagccg tctgtccaat tacgaagtag ttcgtgagaa aaaagaacgg
     3481 acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg aaatcgcgca agtgcaactt
     3541 tcaacgacaa caggaagtat ctatttaaaa gatacagata aataaagagg ttgtgaaaga
     3601 gatgttcagc cttgagcaat aagaagcgat tttgtaaaat agcttcccct attttacaaa
     3661 attgtgcctt attgccgata ggctatctct tgaacaccgt ttaccaagag attatgaact
     3721 tgccaattat cgccgagtca caaggaataa tttcaaaaaa agctcgtcac gttttatgaa
     3781 tatggtaatt tgtgaccgag aattttgcaa aaagacaccg attaagaaga aggttgtcac
     3841 aacgattttc ttcatttaaa atagtagaac agaggtgaga catgatgaaa aaaaagttga
     3901 caaaatccaa taaaaatgtt gttttgacag ggactttagc aggaatagga gaatacatcg
     3961 ggattgatcc gactgttatc cgtgtgcttt atgtattcct gagcttagtt gcttttggaa
     4021 gtccaatcat tttatacatt ttattagctt tgattatccc atctgcgcgt tctgcacgaa
     4081 caaactatgg acataacaat gcatattatg aaaaaaacaa ttatcgtgaa caaactcgtg
     4141 aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga tgacgaatgg agtgactttt
     4201 aatgtcgtac tttcaacgta ttttagttaa tacgttaaca ttcatatcat tggctgttct
     4261 ttttcctgcg atggtccatg tgagcagtat ttggacggcc gttcttgcca gcttcgtatt
     4321 gtctgtgttg aatatgtttg tcaaaccgat tttgacgata ctgtccctgc cctttacatt
     4381 agtcacattg ggattgttta cgtttgttat caatgcagcg attcttaaaa tgacctcctt
     4441 ttttgtggga gcagaacatt ttagtttttc ttcattcggt gcagcgttgt tagtagctat
     4501 cttgatgtcg cttgtcaatg taattgtcag cgaacataac agtagaaaat aaaagaaagc
     4561 tgtgccgaac tatcggcgca gtttttttat ttagaatcag gaatgcgttt tctattacat
     4621 tggtaattaa ataaaaacgt tttttttaat tggtagtact tttggctaga caatgataaa
     4681 atgatagaga acactctaat agaaatgagg tagcatgaat gtcagaagtt gtaaaagtta
     4741 gccaattagt gaaaaaatta tctttggaaa tcgtgacagg cgacgagaaa agcttagacc
     4801 gtgtcatcgt aactggggac atctcacgcc caggactaga gttgactggt tattttaatt
     4861 attattccca taatcgttta cagctatttg gcagtaaaga aatcagtttt gcggaacgga
     4921 tgatcccaga agagcgtttg atcatcatga gaagattatg tagtgaagat atgcccgcat
     4981 ttattatttc aagaggatta ccagctccag aagaaatgat ccaggcagca aatgaaaatg
     5041 ggatagctgt actgcgttct cccatctcaa cttctcgctt attaggagaa ttatccagtt
     5101 atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg tgttttagtg gatgtttacg
     5161 gattaggtgt actgattcaa ggtgacagtg ggataggaaa aagtgaaaca gcgttggagt
     5221 tgatcaaacg cggtcatcga ttgatcgcag acgatcgagt agatgtttat cagcaagatg
     5281 aattaacggt gatcggagaa ccgcctaaaa tcctggaaca tctgattgaa atccgaggaa
     5341 tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc ggtacgtggc tctatgcaag
     5401 tccagttggc tgtttatctg gaagcatggg caaaagataa gaaatacgac cgtctgggct
     5461 ctgaagacac gaccgttgaa atcgcggaag taggcattcc acaaataaaa atccctgtca
     5521 aaacaggacg aaacgttgca atcatcattg aagtagccgc gatgaatttc cgtgcacgta
     5581 caatgggctt tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg attgaagaaa
     5641 actcaggcaa cgactagcaa ggagtgacaa tatgttagca caaatcaatc gagttgcttt
     5701 tgagcttgta gggattccta tctactggta tgcattgatc atcgtatcag gtatcattat
     5761 cgccatgtgg cttagcagca gagaggctgt tcgggtagga ttaaaagcag atgatgtgac
     5821 agattttatg ttgtggggat tgcctatctc cattattggc gctcgcttat attatgtttt
     5881 atttgatctt ccccagtata tagctgatcc aatccaaata ttcaatatcc gttcaggagg
     5941 cttagctatt tatggcggac tgattgcagg cgcgatcacg ctgtattttt ttactaaata
     6001 tcattttatt tctttgtgga cttttttaga tatcgctgca ccaagtgtat tattagcaca
     6061 agcaatcggt cgttggggaa atttcatgaa ccacgaagca tatggaccag acacgacgcg
     6121 aagcttctta gaaagtctgc atttaccgca attcatcatt gataatatgt atatcgaagg
     6181 gatctatcgt caaccaacat ttctttatga atcactttgg agtttgattg gatttatttt
     6241 gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa ggtgaagtcc ttttatgtta
     6301 tctaatgtgg tatagtttcg ggcgtttctt tattgaagga ttacgtacag acagtcttta
     6361 ctttttaggt gtgatccgta tttctcaatt attatctgct gtgttatttg tcggtacatt
     6421 gattctattt atttggcgca gaaaaaaagg aaatttaccg gattatgacc gctcattagg
     6481 aaaaaatcaa caaattatct gatttttaga gcgttaaaaa tgaaaaaaat ttcaggagtg
     6541 gtaaaatgaa ttatagagta gcggttttag gaccaggctc ttggggtact gctttggcac
     6601 aagtcctcgc cgaaaacgga catgacgttc ggatttgggg acatcgtgct tcacaagtag
     6661 aagaaatcaa cacccaacac acgaatcaac gttatttacc agatcattgt ctacccgatt
     6721 ccatccaagc attcaccaat atggaagatg cagtaaaaga cgcagatgcg gtgctattcg
     6781 tcattccaac gaaagcaata cgttctgttg caaaagaact agtgcctaaa ttgaatacaa
     6841 agccagtgat catccatgca agtaaaggat tagaacaggg gacacataaa aggatctctg
     6901 atatcttgtt agaggaaatc ccagtagaaa aaagaaaagc tgttgttgtt ctttctggac
     6961 caagccatgc agaagaagta gcgaaacatg acatcactac gatcacagct gcaagtacag
     7021 atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa taattatttt cggatctata
     7081 ccaatcctga tgtgatcggt gtagagatgg gggcagcttt gaaaaatatt attgctattg
     7141 gtgctggcgc tattcacggc ttagggtttg gcgataatgc aaaggcagca atcatgacgc
     7201 gcggattagc tgagatcagc cgtttaggtg tagcaatggg tgccaacccg ctgaccttta
     7261 tcggattaag cggagtaggc gatctagtcg ttacttgcac aagtgttcat tctaggaact
     7321 ggcgcgcagg caacttgtta ggacaagggc agcccctaga agaggtgcta gccaatatgg
     7381 gaatggtcgt tgaaggtgtg aatactaccc aagcagcgat ggaactagcc caaataatgg
     7441 aaatcgaaat gcccatcact caaacaatct atgaagtgct gtatgaacaa aaagaaatca
     7501 aaacagccgc aaaagaaatc atgttacgtg atgggaaaaa agaaaatgaa ttctcattaa
     7561 caattttttg aaggagcgaa tgtatatgaa agtcaaaaaa gcagttattc cagcagcagg
     7621 attaggaaca agatttttac cagcaacaaa agctatggca aaggaaatgt tgccaattgt
     7681 tgataaacca acaatccagt tcatcgttga agaagcgttg aaatcaggta tcgaagacat
     7741 cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat cactttgatt caaatattga
     7801 attagaaaca aacctaagtg aaaaaggaaa gaccgaatta ttgaaattag tagaagaaac
     7861 gacagatgtc aacttgcatt tcatccgtca atctcatcca aagggtttag gacatgccgt
     7921 tcttcaagca agagccttta ttggaaatga accatttgtc gttatgctag gcgatgatat
     7981 aatggaagac gaagtaccat tgacacagca attaatcgat gattacgaac gtactcatgc
     8041 ttctacgatc gccgttatgc aagttcctca tgatgaaact tcaaaatacg gaattatcaa
     8101 tccaggagaa gtattggaag atgggttata caatgtcaaa aactttgttg aaaaaccaga
     8161 tccaagcaaa gcaccaagtg atttagctat tattggacgt tacttattga ctccagaaat
     8221 ttttgatgta ttgtccgagc aacaaccagg agccggaaat gaaatccaac taactgacgc
     8281 aatcgataca ttgaataaaa cacaacgcgt atttgcccgt aaattcacag gtaaacgtta
     8341 cgatgtcggt gacaaatttg gttttatgaa aacaagtatt gaatatggtc taacacatcc
     8401 agaagtaggt gcacctttac gtgagtacat cctgtcttta ggtaaaacat tagaagcaga
     8461 aggaagcaag atcgatcata atccaaaagg aataacagaa atcaaagaag aaaagaaaaa
     8521 ataacccttt tttctaacga gcttcagctg ctaattgaat aaaaagtagt tgaagctcgt
     8581 ttttatttgt ttaaaaaaca cgaaaatgct atccttatag aaaagagaga caagaaggga
     8641 gtttgatcat gtcaggagga gaaattgcag gattgatcgc agcaatcgcc tttgcgatac
     8701 ttgtcgtttt tatcgttcgt gtgttgttgc aagtttctaa aactttagaa aaagtagatc
     8761 agactgtagc agaagcaaat acgacgatcg aaatcgtcac aaaagacgta gatcttttgt
     8821 ctagacaagt agaaggctta ctggtcaaaa gtaatgaact gctcttggat atcaatggaa
     8881 aagtcgcaac gatcgatcca ttgttcacag cagttgctga tttaagtgaa agtgtatcag
     8941 aacttaatca ttctagtaaa aacatcgcga cgaaagtcgg cggaatcggt aaaacgacag
     9001 ctaaagcgac ggttgccgga aaagtcggtg ggacagcgat gaaatttttt aaaaacaaaa
     9061 aacatactga aacgacagga ggaaaataaa aatggctaaa aaaggtggat ttttattagg
     9121 tgcagtagtc ggaggcacag ccgcagcaat cgccgcgtta ttgttagcac caaaatctgg
     9181 taaagaactt cgggatgaat tagctgccca agcggatgat ttgatggata tggcttcaga
     9241 ttacacagat atggcgaaag aaaaatcgac tgagttaaca ggtattgcaa aagaaaaagc
     9301 gcaagatctt tctcaacaag caggtgatct agcaggtagt gtcaaacaaa aagtaagtga
     9361 atcagcagat gaagcaggaa gtgaatctga cgatatcttg ttaaatctta aagaacaatc
     9421 agctgactta tctaatcgtt tcaaaaaaac ggcagaagat atgaaaaaac ctgcagatga
     9481 attaggaagt attgctaaag atacttcaga agatatcatg attgatgtaa aagacgctgc
     9541 tggtaaagca ggagatactg tcaatgaagg aatgtcagaa gcaaaaccag ttgcagatga
     9601 ctcaaaagaa atgatgaaaa tggcaaaaga agatgcgaaa gaagctaaaa aagaaataaa
     9661 agatacaatg acagattcat ctacaaatca ctaaaatcac aaatttatag aataaaaagg
     9721 tgggagcgga ctagctctca ccttttttac tcttattttt ctaaaacaag taattctttt
     9781 ggtgtagtcg tgaatggcac acagccatcg cttgtcacat atacacagtc ttcgatacgt
     9841 acccctactt tttcaggaat atagattcct ggttcaagag agaagcacat cccttcttcg
     9901 attactaagt catttccttc tacaagggaa gggaattcgt ggacggttgt tccgatcccg
     9961 tgacctaaac ggtgattgaa atattcgccg tagccatagc tgttgatcac gtttcgtgcg
    10021 atttgatcaa gttcacctgc tgtcacacct ggacggacgg cttcttgtgc ggcaagctgt
    10081 gcttcaagag tgatgttgta gattttttct tggaagtcgg ttggtttttg ataagcaatt
    10141 gtacgagtag catcactgca atagccattc caaacaacgc caagatcgaa taacacaagt
    10201 tcattcggtg aaatcttcgt atttcctggt gtaccatgag ggcttgcagc atttgctcct
    10261 gcaaggacta atgtgtcgaa agacatttgt gaaacacctt gttttttcaa ttggtattca
    10321 atttctgcaa tgatcgcttg ttcagtcacg ccttctttca ctgctttaaa gccaatctca
    10381 aatgcaacat cggcccattt accagcttcg atcagtttgt ctttttcctc tagagttttg
    10441 atcagttgta gacgttggat gatggaagtt agatcagtgt aagaatccgt tcccgtcaag
    10501 atatcagaga tagcatcgaa gcgctcgaca gtcaaggcgt tcttttcaat ccctacattg
    10561 ccaggctgac catagcgagc acgaagttct tttccgatga tctcccaagg gttttcgctg
    10621 tctaaatagc cacggacatc atacgcccaa ctactgtttt gagcatcttc aacttccaaa
    10681 gcaggagtga aaaggaaagg atcttgttca agagggataa aaagagctag tacacgctca
    10741 tgaggatcac tttcgtatcc ggaaaagtaa gcgatgtggc cagggtcgct gatataagcc
    10801 aattcgactt ggtgttgtgc catccattga cgtagttcat tgatttttac gttattcatt
    10861 tggcaaccta ctttctaaat taattttgta ttcaataatt gtaccactga tttattaaaa
    10921 gaccaacgtt ctaaaaactg aaaaaagtaa aaattttcat gaaaacagag ataaaacggt
    10981 tgcaattctt gagattattt gatattctat tttagaaatg taagacgtat tttcagaaaa
    11041 catataaaat atgaatagga gaacacaaat ggaaaagcaa acaatcacta tctatgatgt
    11101 agcgagagag gcaaatgttt cgatggctac tgtttcacgt gtcgtaaatg gcaatccaaa
    11161 cgttaaacca gcaacaagaa aaaaagtctt ggaagttatc gatcgcttgg attatcgtcc
    11221 aaacgcagtt gcgcgtggct tggcaagtaa gaaaacaaca acagttggtg taatcatccc
    11281 tgatgtcagc aatatgttct ttgcttcttt agcacgtggt atcgatgatg tggcaacaat
    11341 gtacaaatac aatattatct tagcaaattc agatggcaac gaccaaaaag aagtcaatgt
    11401 attgaataac ttactggcta agcaagtgga cggcgtgatc ttcatgggtc accatatcac
    11461 agacgaaatc cgtggagaat tttcacgttc aaaaactcct gttgtattag ctggctctat
    11521 tgatccagat gaacaagtag gtagcgtaaa cattgattat gtatccgcaa caaaagatgc
    11581 agtaaacaaa ttggcaaaaa gcggcaataa aaaaatcgct tttgtcagtg gtgcgttgat
    11641 cgatcccatc aatggacaaa atcgtctgaa aggctataaa gaagcattaa gcgaaaataa
    11701 cctaccatat agcgaaggat tgatctttga agcacaatac aacttcaaag atggtctatc
    11761 tctagtcgat cggatccata atagcggtgc aacagctgtc tatgtttcag atgatgaatt
    11821 agccatcggt attttggatg gtttattaga ccgtggcgtg aaagtaccgg aagaattcga
    11881 aatcatcaca agtaacaatt cactactgac agaagtagct cgtcctcgtt taacaagtat
    11941 cacacaacca ttatacgatt taggggctgt atctatgcgc ctattgacga aaatgatgaa
    12001 taaagaagaa gtcgaagaaa aaacgattat cttgccttat ggaatcgaag aaaaaggatc
    12061 tacaaaataa taaaaggaca accattcaga ggttgtgtgc ctgccgttta actctgcgtt
    12121 acaaggagca atttcaaaaa acagcatctc atgtttgatg aatattgtga ctcgtgat
//
LOCUS       DACIRY010000080        12171 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.081, whole genome shotgun sequence.
ACCESSION   DACIRY010000080 DACIRY010000000
VERSION     DACIRY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.081"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            592..1920
                     /locus_tag="IYE22_001865"
     CDS             592..1920
                     /locus_tag="IYE22_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ9507072.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IYE22_001866"
     CDS             2122..2955
                     /locus_tag="IYE22_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ9507073.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IYE22_001867"
     CDS             2971..3708
                     /locus_tag="IYE22_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ9507074.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="IYE22_001868"
     CDS             complement(3863..4831)
                     /locus_tag="IYE22_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ9507075.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="IYE22_001869"
     CDS             5063..5896
                     /locus_tag="IYE22_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ9507076.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="IYE22_001870"
     CDS             5930..6769
                     /locus_tag="IYE22_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9507077.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="IYE22_001871"
     CDS             6795..7355
                     /locus_tag="IYE22_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ9507078.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="IYE22_001872"
     CDS             7554..9275
                     /locus_tag="IYE22_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ9507079.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="IYE22_001873"
     CDS             9440..10582
                     /locus_tag="IYE22_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ9507080.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="IYE22_001874"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="IYE22_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ9507081.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DACIRY010000081        12043 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.082, whole genome shotgun sequence.
ACCESSION   DACIRY010000081 DACIRY010000000
VERSION     DACIRY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.082"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(229..435)
                     /locus_tag="IYE22_001875"
     CDS             complement(229..435)
                     /locus_tag="IYE22_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507082.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(451..1065)
                     /locus_tag="IYE22_001876"
     CDS             complement(451..1065)
                     /locus_tag="IYE22_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ9507083.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1212..1895)
                     /locus_tag="IYE22_001877"
     CDS             complement(1212..1895)
                     /locus_tag="IYE22_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ9507084.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(2004..2462)
                     /locus_tag="IYE22_001878"
     CDS             complement(2004..2462)
                     /locus_tag="IYE22_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ9507085.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2546..4309)
                     /locus_tag="IYE22_001879"
     CDS             complement(2546..4309)
                     /locus_tag="IYE22_001879"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ9507086.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4530..5990)
                     /locus_tag="IYE22_001880"
     CDS             complement(4530..5990)
                     /locus_tag="IYE22_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507087.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6198..7124)
                     /gene="glsA"
                     /locus_tag="IYE22_001881"
     CDS             complement(6198..7124)
                     /gene="glsA"
                     /locus_tag="IYE22_001881"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ9507088.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(7147..8574)
                     /locus_tag="IYE22_001882"
     CDS             complement(7147..8574)
                     /locus_tag="IYE22_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9507089.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8605..9819)
                     /locus_tag="IYE22_001883"
     CDS             complement(8605..9819)
                     /locus_tag="IYE22_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ9507090.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(10160..10402)
                     /locus_tag="IYE22_001884"
     CDS             complement(10160..10402)
                     /locus_tag="IYE22_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ9507091.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10434..10991)
                     /locus_tag="IYE22_001885"
     CDS             complement(10434..10991)
                     /locus_tag="IYE22_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ9507092.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(11004..11192)
                     /locus_tag="IYE22_001886"
     CDS             complement(11004..11192)
                     /locus_tag="IYE22_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ9507093.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(11205..11771)
                     /gene="amaP"
                     /locus_tag="IYE22_001887"
     CDS             complement(11205..11771)
                     /gene="amaP"
                     /locus_tag="IYE22_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ9507094.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3542 a         2471 c         1964 g         4066 t
ORIGIN      
        1 attactatat tctatttacg agttcatgat agacttttcc taatgattag gcaatggaat
       61 ttgaaatgaa atagtgaaat ttaacaatct ttaatatttt ttagaaaaaa ataacaaata
      121 agttgaaaga atcccgttgc aaattctttc agttgcacca ggattctttg ataatttttt
      181 agttttatgt gtttttttat atgttttttc acttttcgga ataaatattt atttgttaaa
      241 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
      301 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      361 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      421 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      481 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      541 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      601 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      661 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      721 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      781 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      841 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      901 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      961 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
     1021 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
     1081 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
     1141 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
     1201 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1261 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1321 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1381 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1441 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1501 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1561 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1621 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1681 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1741 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1801 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1861 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1921 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1981 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     2041 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     2101 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     2161 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     2221 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2281 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2341 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2401 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2461 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2521 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2581 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2641 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2701 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2761 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2821 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2881 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2941 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     3001 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     3061 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     3121 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     3181 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3241 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3301 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3361 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3421 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3481 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3541 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3601 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3661 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3721 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3781 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3841 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3901 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3961 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     4021 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     4081 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     4141 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     4201 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4261 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4321 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4381 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4441 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4501 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4561 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4621 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4681 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4741 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4801 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4861 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4921 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4981 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     5041 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     5101 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     5161 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     5221 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5281 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5341 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5401 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5461 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5521 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5581 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5641 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5701 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5761 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5821 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5881 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5941 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     6001 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     6061 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     6121 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     6181 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6241 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6301 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6361 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6421 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6481 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6541 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6601 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6661 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6721 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6781 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6841 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6901 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6961 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     7021 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     7081 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     7141 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     7201 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7261 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7321 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7381 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7441 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7501 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7561 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7621 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7681 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7741 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7801 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7861 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7921 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7981 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     8041 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     8101 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     8161 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     8221 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8281 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8341 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8401 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8461 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8521 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8581 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8641 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8701 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8761 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8821 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8881 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8941 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     9001 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     9061 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     9121 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     9181 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9241 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9301 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9361 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9421 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9481 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9541 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9601 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9661 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9721 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9781 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9841 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9901 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9961 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
    10021 ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca tataaaatat
    10081 ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc atttcaacat
    10141 aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact aatactaaaa
    10201 tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt ccccaagttc
    10261 ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt gcgatgatcc
    10321 ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata ccgccgacga
    10381 ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac ttcttattgt
    10441 acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc agattttgct
    10501 ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt tactttgtct
    10561 tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc aacattcaca
    10621 ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat caattctttg
    10681 atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc tagatccaca
    10741 gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc tgtgttgact
    10801 aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc atctacatct
    10861 ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa ttcaccattc
    10921 atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt attcatgttt
    10981 ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat tcctgactgt
    11041 ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa caatgtcttg
    11101 aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc aaagataata
    11161 gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc cactcgagag
    11221 tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac tgccactttg
    11281 accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg catcaataca
    11341 cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg gatatggatt
    11401 cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc ttcgtgtaaa
    11461 tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc atcttcccgt
    11521 ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa gatgataata
    11581 gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta gtaattcgtt
    11641 aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc attgatcaac
    11701 gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat tatttttaac
    11761 cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa ctctaccgac
    11821 aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg ctaccaaaca
    11881 ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa ttgcttactt
    11941 attctctatt tacttttgat tgctggctat acttaaagga tattattcaa gattaaaaat
    12001 tgcaaagatt atgctcagtt attattacaa gattctattt att
//
LOCUS       DACIRY010000082        11592 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.083, whole genome shotgun sequence.
ACCESSION   DACIRY010000082 DACIRY010000000
VERSION     DACIRY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.083"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            395..601
                     /locus_tag="IYE22_001888"
     CDS             395..601
                     /locus_tag="IYE22_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ9507095.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(803..1804)
                     /locus_tag="IYE22_001889"
     CDS             complement(803..1804)
                     /locus_tag="IYE22_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9507096.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1809..3722)
                     /locus_tag="IYE22_001890"
     CDS             complement(1809..3722)
                     /locus_tag="IYE22_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ9507097.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3890..4396)
                     /locus_tag="IYE22_001891"
     CDS             complement(3890..4396)
                     /locus_tag="IYE22_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9507098.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4556..4996
                     /locus_tag="IYE22_001892"
     CDS             4556..4996
                     /locus_tag="IYE22_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ9507099.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5022..6179)
                     /locus_tag="IYE22_001893"
     CDS             complement(5022..6179)
                     /locus_tag="IYE22_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507100.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6182..6538)
                     /locus_tag="IYE22_001894"
     CDS             complement(6182..6538)
                     /locus_tag="IYE22_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507101.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6836..7810)
                     /gene="sgrA"
                     /locus_tag="IYE22_001895"
     CDS             complement(6836..7810)
                     /gene="sgrA"
                     /locus_tag="IYE22_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ9507102.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8006..8845)
                     /locus_tag="IYE22_001896"
     CDS             complement(8006..8845)
                     /locus_tag="IYE22_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ9507103.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9030..9917
                     /locus_tag="IYE22_001897"
     CDS             9030..9917
                     /locus_tag="IYE22_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ9507104.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNNDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10511..11206)
                     /locus_tag="IYE22_001898"
     CDS             complement(10511..11206)
                     /locus_tag="IYE22_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ9507105.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11190..11588)
                     /locus_tag="IYE22_001899"
     CDS             complement(11190..11588)
                     /locus_tag="IYE22_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ9507106.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3458 a         2305 c         1735 g         4094 t
ORIGIN      
        1 tctaaaaaat taaaaaaaat tttggtgaat taagtatttc cttgataaca ctggcttttg
       61 gcctcatttt aggttatcat ttgtaatttt atttcacagt tgatatgatt gttatgtaaa
      121 ataaaaaagt ttaggataca tcgaattttt ggggaaattc ttttgggggt atcctaaatc
      181 ttttctatta tattcccata ttttctatta ttggtctaga cgtatgatgg taaactttgt
      241 acctatctga ttgctgaatc attaattgct tatcattagc catctttgtt tcaagttcgc
      301 tagatgatca ttttattcac atcctaacaa acttgtcaca tttatagatt cttaatctat
      361 ttatcataca attataaaaa aggaggtgct gcttatgaaa gctatccata tttctttgct
      421 ttttttcgct agtgcattga ttttatttgc tattcatctt tgttcccagc cttatatcaa
      481 taacaacgga atattagtgg aaccagcatt cttttgctta ccactcggct ttttgtccct
      541 tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa cgatcaaaac aacaaatctg
      601 acttataaaa tttacgtaaa agagatcgtg acaataatcg tgtgatattc ccaaatttcg
      661 aataaaaggt gccccttgcc atctctaact cctcagtcac aagttacaat attcataaac
      721 catgaaaagc ttgtttattg aaattgctcc ttgtgacttg gagctaagtg acagattaca
      781 acctcttttg catcataaga ctttactcct tgaaaaacat tactaacttt tgatatgttt
      841 cttgcgcttg tttattgctg aaatcaaact gatattcatg gttcaaaaca gcatttttct
      901 ccgcataatc tacttttgtg actttcaccc ccgctttctc aaatacagaa gttacttctt
      961 ccatttgttt cgtaaacgta tttgtatttc catccgttaa gaatgtgttt tgcgtccaac
     1021 gatccgcatg tctgaccaag gctaaacttt tgaccttctc tgattgttca aagtccttat
     1081 tatcaaagta agcccatcca catttttcat aaagaaagct agagatttta tcacttactt
     1141 gagtaagtgc agaaggatcc aacagaccag acaaagaaat aaacttcgta atctgcttgt
     1201 tctccaaagt cggatcaaga tgatcagctt ttttaatttc ttcattttga agtgctaaca
     1261 aatattctcc actaatttgt gcacctgccg aatttccacc aatcgaaata ttcgaccaat
     1321 ctgcttgatc ttttatctct tttttagctg cttgaatggc ttcgttcaat tggataactg
     1381 gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat atttacgatg atttgctgcg
     1441 tgtctgaaca tagatattcc aaataatcac gacaatcatt tttatcccct cctacatatg
     1501 cgcctccatg catccagaaa agaatcggta attttttctt ttccactttc ggtgtgtaga
     1561 tatccatatt attatttttg tactttgaag gataggatat ttcttttttt atatcgattt
     1621 gttcgatttt ttgctggaaa tcatctggtc gggcatatac ttgttctttt tcacttgtgt
     1681 caaattgttt tctaacaagc aagctaatcg gctttggtgt gatttgatta atcaaaaata
     1741 cagtgactaa aataactacg atgataactg gtactgcaat cactattcgc ttgaaccatt
     1801 tcattcattt accctctctt tcttcgccaa atccatgtga taatcgtgct aacagataaa
     1861 aatccacctg aataaatgag aagcgaatgt ttcttttctc cagttttcgg caacattctt
     1921 ttttttgcat ctgtcttttt ttgtactttc actgtcatct gaatcggttg cgtatcatca
     1981 cttgattgat cctctcgagg ttcattcttt cccttattgt ctgcattctc tgtcggatct
     2041 tgcttttttg gtggagctac tgggattttt ttgtaataga aaataatagt tggtatcgtc
     2101 tcattaaaaa tcccttcagt cggtccttca acatttgcta attcgtatcc ttcgaatgtc
     2161 ttttgctctg tcatataatt ttctccataa gctccgtaaa gctggatttc gtcctggtct
     2221 ttgagtagac ttttattgtt ttcatccaca tactttacgt ttatctgatc atggaacact
     2281 actttcaaaa agtccgttac atgtttcgtg taggcagcat ggtcaacttc ctgtgccttt
     2341 ccatgccctg ctccttctac taataaaaga tctttatacc ctcctgataa ctcatataat
     2401 ttttgcgcgt tagacacagg tactacatca tctgctgttc catggataat caatttaggc
     2461 aatgaatctg acattccaat cgtaggtaga gcagcttttg tatccaaatg catctcctga
     2521 tcaaaatagt attgtttcaa aaggcccata aataaatctt catgctcttg atccccaatg
     2581 atgtctaact gattttttat ggcatccggt agtgcggggg ctactgtctg gctgtacgta
     2641 aaacgtagtt gttcatcaat tgatgcaaat ccgcaatttt caataattcc tttgacattg
     2701 acaggaatat ctttcgctaa tacagacatc atcgtagctg ctcccatcga accaccataa
     2761 agtaaaatct tttgagatgg ccgctctcga acttcttgat ttatccagta aaggacgtca
     2821 tctgactcat attgtccata ggtcacataa tcgcctccgc tattaccagt cgctctggaa
     2881 tcaacaaata ataaattata cccggcctta taaaaatcat tgtattcttc tgtaacgatc
     2941 ccgttattcc aattccctcg aaatccgcca tggatcatga ctgttttatc tgaaccttga
     3001 tcgacataat atgcatatac atccatcatt ttgccatctt gtttgtttag tacagtttga
     3061 tgaagttgtt ttgtatttgg atagaagcct gtgttttctg taggcgttgt cccatcatct
     3121 gtacctccaa gcatcgacat caacacactg ttttttgcaa aagcgatatc gtacaataga
     3181 taagtagcaa tcagatgttt gataatctct ttatctacac cataatcatt aacagcaagc
     3241 tcagtgacta ataactctat taattttgac ttgatgtctt ctgttggtcc attaagtatt
     3301 tctgtcatct gtttaccgaa gtaattcaaa tattctttac cattgaaatt ttcaccttta
     3361 ccgatatcat cattttctgt cccattctct ttgtcactta ctttagcaga agaggattcg
     3421 tcgtttgaca tttttgtagc tgttgcttct tctgtttgtt ggttatctgt ggtagcggtc
     3481 gtttcttgat ctgtgctagt aacagatgtc tcaaccgttg tttcagtatt tgtttctgtt
     3541 tgtgaagtac tcgatttcgt ttcatttcca gcatcagtag tttctgtggt ttctacctcc
     3601 cgagattccg attcggaaga aatgctctct ccatatactg tccattgcgg gacgcttgta
     3661 cttacagcaa ataagatgac caacgctccc tttaatactt gttttgttct caagctattc
     3721 actccttttt atgtttctag gagaattata tgtaaacgca aacatttttt atacaaaaaa
     3781 taaatggttt atcccataaa aatagaacag gattattttc tcattttacg taataaagca
     3841 gaatggataa accagtatcc attctgcttt ttttaaacga agaaacaact tattgctcca
     3901 aaaatgtcag aaatcgttta tcccaaattt tctcgtaaaa agcgattgtt tcctttgctt
     3961 tcataaaagg attatcataa gttgtcgttg ctcctctaca catcataagc tggtatccta
     4021 atccatgagc aaacttcaca gtcgtatcta tacaatactc tgcttgtgcc ccacataatt
     4081 cgatcgaatg acagtccaat tcagttaata aaacactcaa attagtctta taaaatgaat
     4141 tcgcatgggt ttttctcaca aaataactgt gctcaggaat agataattcc gacaatagct
     4201 cccatggttt tgtttgtggc actaattgag catcttcgtg ttgaacgaaa ataactggtt
     4261 tcccaatcgt ttcgtatgtt ttgattcttt tattcactaa tgctgtcact gtttctaatt
     4321 cgtgtatcgg cgaagaatca aaacataccc ctttttgtaa atcgatcact actattgcgt
     4381 cggctgtgtt tgacatactt gtgccttctt tcttttttct tgatttaata gtatttctta
     4441 tctagctagt tccattatac gtgacaacat tttcttattt tacacctcta aatggcactt
     4501 ttcgactatt tcttcatgtt attatttcat aaatacttag ctattggagg aatgtatgat
     4561 gattaaattg tatacaggtt ctggctgctc gtcttcaaga cgcgcaaaac gttggcttat
     4621 cgtccatgat ataccgttta tcgaacagaa cttaattcgt caaccactaa ccaaaaatga
     4681 atttatccac ttactaaaat tgacaaacaa cggcgtgacc gaaatgctct ctactcattc
     4741 tattagttat aaaaatcttg ttcacagaat agatgaactc agtttgagtg aactatatat
     4801 tgagattcgt acatcccctt ctttattgaa aaaaccgatt atctacgacc aatctgcaaa
     4861 acttttgact ggatttagcg aagataaaat caggatgttc ctttctaaaa ctaaaagaaa
     4921 gcaagagctc aacgaactct tgctttctgg tcatcttgtc gattttaaag aaaataatct
     4981 ttttgcaatg tcttaatttt ggcataaaca atctgctttt cttaattaac gaaatccaat
     5041 ttttccaata tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc
     5101 gcccaatcat tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca
     5161 tatgcctttt tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat
     5221 gcactatatt ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc
     5281 tgtgttttac catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa
     5341 atatccctga ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta
     5401 ttaagctctg gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc
     5461 actggaaata tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc
     5521 ttttttgtcc caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa
     5581 tttttttctg aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac
     5641 gattcaatcc agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc
     5701 aaataggtgt tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg
     5761 tgattcatcg accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct
     5821 tcttttgtta tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag
     5881 tggccttgtt tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct
     5941 atactacgcg tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt
     6001 atacttgata atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa
     6061 aagtatcgga tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat
     6121 aaatgatagt ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa
     6181 cttatataat tttttgacta agattttcta aggattgata agtacctttg taaagtgaat
     6241 ccagtaaaat ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa
     6301 actgagaaga aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt
     6361 ctggatacct ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg
     6421 tgtagtttag atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac
     6481 aatagttcaa aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag
     6541 tacatactcc tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca
     6601 aatagattcg ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat
     6661 tcttgctctt attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa
     6721 acaaaaatag atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa
     6781 tattcactca aaaatctatc gttttattca attttagatc tgttgctttt attctttatt
     6841 tcactcgatg gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac
     6901 ccatccatgt aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc
     6961 tattatctgt ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct
     7021 gttttagcac actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga
     7081 taggtaccaa atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc
     7141 ctgtgctact ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac
     7201 tgctttcgct gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt
     7261 cgctgctcgt gttttctgtg ctactttcac tgctcgtact ctctgtactg ctttcaccgc
     7321 tcgtgttttc tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa
     7381 ctgtccaact gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa
     7441 tcaaatcaac tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta
     7501 ttgtatactc aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt
     7561 tttctgaact tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat
     7621 tcacgctttt gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa
     7681 tccgatcagt tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac
     7741 tgattccaat cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg
     7801 ttttcttcat ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata
     7861 aaatgaaaat gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta
     7921 ttcgcttgaa tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg
     7981 atatcagcct tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct
     8041 ttgttttata tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg
     8101 atatagatgc ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca
     8161 gagtggagaa aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt
     8221 gtgtccaatt ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga
     8281 taagtgcgct gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt
     8341 taggattcaa tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg
     8401 gaccaaatct aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc
     8461 ccactgcaat aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat
     8521 ctaaccgata cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc
     8581 caacaacgag acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat
     8641 cttctattct ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta
     8701 aatgtccgac cgtaccggca attgctccaa tccattcact atttccaata aacatatccg
     8761 caagtgcatt attgacaaga attcctaaaa caaaaccaac taagactatt aaaacagatc
     8821 caatcaaccg aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc
     8881 ttttctttct tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt
     8941 ttacgaacta agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct
     9001 tctgttagac tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa
     9061 ttatgtgcct ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa
     9121 cgattgcaac gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag
     9181 ttaaatcaaa taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg
     9241 tatacggaaa agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa
     9301 aactcggtcc aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata
     9361 agaaactctt taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa
     9421 cagataaaga tctagacact gcttggaaag aattctatcc agaagttagt acacagatca
     9481 ttctattttc gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggagaaa
     9541 atttctccaa gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa
     9601 aaatgaattt tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta
     9661 aactaaaaaa cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc
     9721 aaagctatta tctagtaaaa atggtaaaaa aacaggataa aggttctaat aatgacaaat
     9781 acaaatctga gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg
     9841 ataaaacaat ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa
     9901 aaaatatatt caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg
     9961 atttttggaa attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg
    10021 ccttttttta tttgtaccaa aacaaatcac tggatttgtt acaatcatga ggatataatt
    10081 taaaaaagtt ttttatttct atcacatgaa tcaatgcgcc attaagtaga ttctttcatg
    10141 cttccctatt ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg
    10201 actgaaaagt tattttaaaa aagactaaaa atatcccttc tggacttgtt cttcttattc
    10261 tgttatacta gagaaacacg aaagatattt ttatgcgaca cttttcattt gtttttatga
    10321 aatttattca aaactggcgt aacttcagag ttacaaggat gtattagagg ttagggaata
    10381 catcgtttaa atgagaaata agtaatagtt agtaatagtc acccaaactt taaattaatg
    10441 aatattgaat cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt tattttatgg
    10501 agaaattgat tcagaagtaa tcagcgacct tttttagttc accaatcgtt tctgcttgga
    10561 aagtttgcac ccaatcttct tggaagcccc tcactgcttt atcaatcgag ggcatctcta
    10621 atacttgatc tagtagctct ttacctaacg ttatcgcatg agctccagat cgaatggctg
    10681 tagtgacttg ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac
    10741 gttcgatctc agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca
    10801 tcctgttata ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta
    10861 attctgtgta aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg
    10921 cttgaaggcc tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta
    10981 tgataactcg agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga
    11041 taggaatctg tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc
    11101 cttctttttt cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac
    11161 aagaataaat actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca
    11221 ttctgatcct cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta
    11281 tcttctacat acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg
    11341 atatgtccta attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac
    11401 atttgtttat caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat
    11461 tcaatcttat aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt
    11521 attttttctt tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag
    11581 atcgtcatct ct
//
LOCUS       DACIRY010000083        11491 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.085, whole genome shotgun sequence.
ACCESSION   DACIRY010000083 DACIRY010000000
VERSION     DACIRY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.085"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(165..755)
                     /locus_tag="IYE22_001900"
     CDS             complement(165..755)
                     /locus_tag="IYE22_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ9507107.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1278..1439
                     /locus_tag="IYE22_001901"
     CDS             1278..1439
                     /locus_tag="IYE22_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507108.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1458..1952
                     /locus_tag="IYE22_001902"
     CDS             1458..1952
                     /locus_tag="IYE22_001902"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAQ9507109.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            2590..3613
                     /locus_tag="IYE22_001903"
                     /pseudo
     CDS             2590..3613
                     /locus_tag="IYE22_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3956..4212
                     /locus_tag="IYE22_001904"
                     /pseudo
     CDS             3956..4212
                     /locus_tag="IYE22_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            4599..4826
                     /locus_tag="IYE22_001905"
     CDS             4599..4826
                     /locus_tag="IYE22_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ9507110.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4842..6008
                     /locus_tag="IYE22_001906"
     CDS             4842..6008
                     /locus_tag="IYE22_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ9507111.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6243..7286
                     /gene="hrcA"
                     /locus_tag="IYE22_001907"
     CDS             6243..7286
                     /gene="hrcA"
                     /locus_tag="IYE22_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ9507112.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7380..7943
                     /gene="grpE"
                     /locus_tag="IYE22_001908"
     CDS             7380..7943
                     /gene="grpE"
                     /locus_tag="IYE22_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ9507113.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7994..9823
                     /gene="dnaK"
                     /locus_tag="IYE22_001909"
     CDS             7994..9823
                     /gene="dnaK"
                     /locus_tag="IYE22_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ9507114.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9974..11140
                     /gene="dnaJ"
                     /locus_tag="IYE22_001910"
     CDS             9974..11140
                     /gene="dnaJ"
                     /locus_tag="IYE22_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ9507115.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         4037 a         1840 c         2352 g         3262 t
ORIGIN      
        1 gttctgcacc ttataaaatt tgtcaaaaaa actgttgcca ttccatctat gaatataaat
       61 agagtgtatc ttctttgata aatgtcagaa aaagtgtgac aaataaccat cagtaaaatt
      121 acttgtcata ctattgataa ggagtattta attgaagaga atgattagga gaaaggatct
      181 ccgtactttt tatagtaatc atagaatcca agtttgtata gacgaatctt tttttctctt
      241 atttcttctg tttcaaaatg gactgcttga agaatcaagt ctgtgaattc tataggagct
      301 gttccattcg cagtaatcaa attacgatct ttcacagctt gttgttctat aaaatcttct
      361 ttattctgat aattttccga ttctaaccaa ggaaattgcg catttccggt gtgtttgtaa
      421 ccttgcaata atccgctctg agctaaataa tcaactgccc cacaaatagc agctactggt
      481 acgtctgttt ttaacaattt atcgattatt tgatataaat gatgattttt tatgtcccat
      541 gaattgccac cgatcataat aaaaaggtca actttttcag gtatttgttc gattaagtaa
      601 tcgatttttg tttttaatcc tccaatggaa gtgacctctg attggataga agcagttttg
      661 ttttgccatc cctctttttg gtttagtgtg ctagtaatat aagcaccttc ccaatcggca
      721 tattcctcta atagaaacgt aatcgcagtt ttcattttca agctctcctt tactatctat
      781 gacaaggaca acagttttaa catacagaca ttcattaaat ttggatagcg acagcgaaaa
      841 caacaattct taagttttag acagagtata cagacagtca acttaattaa atacgaacta
      901 aataaaaatg acaggtaatc atttttgttt accagcatta taacattttg catacatgga
      961 tgacaaaaga accaattccc acttgcttga gtacaatctt aaaagaaact ggaaggcaaa
     1021 aagtaattat agaaacgaaa aataaatcat atcgggcact cagacatgaa aacaacccta
     1081 gaaatatata actagtccaa tgaaaaaaaa gcgaatgccg tagaaaacaa ggattctgcg
     1141 tacattcata tctcattttg cgagcatatc tgctattact gtgactaaat gttaatatgg
     1201 ttaaaatgac cgttatattt tttatataaa tatcgtataa ttagtatgaa tatagaagaa
     1261 ggaaagaagg tgcaatcttg gaacctaaag acaagtattt agattacgct agggtcaata
     1321 aagataaatt tattgaaaca atcatcaagg gaaaaatccc tgatgatgaa aaagatgcgg
     1381 tattcatggc tggaagccca ggagcaggta aaacagaggt tgcactaggt ttagcttaaa
     1441 attatgacaa ccatgttgtg attgatgcgg actattttag agcacaattt ccagaataca
     1501 atggaaagaa ctcaagtgtt tttcagaaag cttcttcttg gttagttgaa caatcattaa
     1561 aatatgtact ggaacatggc tattctttca tactagagga tacttttgct atccttagtg
     1621 cagagaaaaa tattattcga gcccttaaaa ataactttcg cgttacgatt ttctatgttt
     1681 accaagatcc caaagttgcc tgggatttca ctcgaaaacg tgaactagct gaaggacgtc
     1741 atgtgccaaa agaaatgttt atcaatgcgt tttttaagtc cagagagaat attgaaaaag
     1801 tcaaaaatag acatcctgaa gtagtccttc acataatgat taaggattat caaaacgaca
     1861 tttctgaagt gcattatgca gcagataata ttcaattagt actaccaata caatatactt
     1921 caaaagagtt ggaggaggaa ttacatgact gagaaagaac aagtacaaca aatcgtgaag
     1981 aagtataata aaagtatcgc tgatttatca gaaaacgcct cagcgaaaga atttaaaaca
     2041 gtaatgaatt acgtagcaga tgaagcaaat cgtaagcagc gaaaactagt tgggttggat
     2101 aaataaattt tttaaaccat atccttgtaa agttgagttt tttattttaa tgaattaaga
     2161 ctattaaata gataattatt gtaacttaaa aaacacagct aaaaattagt tgtgtttttt
     2221 attaggttta cttttctggt ataaaaggaa ctagctactt tttcaaaatt tctaaatcat
     2281 tcaatatgta gcgcttacta aattattcgc cataaaattt tataattgga tcactcattg
     2341 ttttacctaa gtcatattta agtaataacc ggtgttttta cataagaaga cttcgggtat
     2401 tttttagttt gattagctct gatataccta taaagaatat gctaaggtaa gaatagtaaa
     2461 aattcacata gatgaggtat aataatgata tttttatata ttacagataa cttgacacaa
     2521 aatgagtttt tttcgatttt tcaagttatc taatgcaatt atattgatat cattaggagc
     2581 tgcattgtta tgaatccaac attgattact aagaaagaat tactaaaaaa attagatatt
     2641 tctactggtg tattagctaa tttaattaga aatggcatgc caaaagaggg agaaatgttt
     2701 aatcttgata agattattac atggcgagaa aattggtcta aaaacatatt aggtgagtta
     2761 gaagttggta gagtatatac taacaaagaa atttcagaga agtttaaatg ttccaaacag
     2821 ggtggaatga gaagatctca tcaaactaat actcttgtac tgtttagtga tcaaacagga
     2881 agtaatgttt ataaagataa gtggttaaat ggtattttac aatataccgg tatggggcta
     2941 aaaggggatc aagtattgga taagaatcaa aataaagttt tagccaattc aaaaagtaat
     3001 tttgtaaaga ttcatttatt tgaaacattt aaaccaaaag aacacacata tctgggagaa
     3061 gtatatttag caggacaaat atatactgtt aatgaaaaag atagttcggg caacagcaga
     3121 aaagtatata aatttccatt agctttaata aatcaagagc aattgattga agataaagat
     3181 atataaccag gaagaaaatc aaactagaca tattcgtaat ttatctaatg ctaaattaga
     3241 ggaagaagca agaaaagtta gtaattataa taaaaaaatg gctgcaaaat atgttaacga
     3301 agaggagttt tgcttctcaa aaacaaaaat atataaacgt aatcccttcg tatctgaata
     3361 tgttaaacga cttgcaaaag gtatttgtca actttgccaa gaaaaagggc catttataaa
     3421 agacggtgta ccttatttac attgtcatca tatagaatat ttgtcgcaag gtggaaaaga
     3481 tgtgattgaa aactgtatcg cgttatgtcc caattgtcat gctagaattc atgaacttga
     3541 gttacctgac gataaagaaa aattgctgaa gatcgttgaa aaaagagaaa atagcttttc
     3601 taaaataccg taatttattt ttataggaca aataagctaa tgtgagctat gccttctata
     3661 taagactaag ttctattata agtaacgaag taattctcgt tccttcctgt agtaatattt
     3721 tttaaataac ctataggctt cttcaaaagt acatcctcca gtaaattcta agtactggag
     3781 gattcttttt ctagtgttgc tttactcatg aaaaagtagt tggctacttc atcaaaattt
     3841 ctgaactttt ttaatgtata ataaagagct cgatcattta ctacaaggat gctagctcct
     3901 acattttcac agttttttag caagtaaatt tttctaaaat cacatcagga aatgcatgag
     3961 attctttaag agaggcaatt atagggctat gattacggtt cgtgtaagtg acgccaaaaa
     4021 agaatggctt tcttatatgg ctgaatttta tgggattact ttatctgaac ttctaaaaaa
     4081 ctattcgatg gaacaactag aagatgaata caatcgtcaa acggctgaaa ttgcccataa
     4141 acattggata agaagtggga aaacaacagt attcatggaa gaatccttaa tgaatttggt
     4201 gggctggaat gacctgtcgt ttaaaattca cgcccgaaat tcagaagcag ctctgattgg
     4261 caaatacgtg tattggtaga gatagaagat aatcaattcg ttgttatcgc tattcaagta
     4321 gggcaccgaa gaaatgtcta tgattaagag actgtttatt tttacaaaaa aataataatt
     4381 ggaatctagt taaaactctt gaagtgagag atttgactag atttttttat gaataaaata
     4441 aggacaaaat tgtttaggaa gaatcatgag tgtaaaaaaa tcatccaaga agtcgattct
     4501 tggatttttt agtggatttg ttcgccaaat acgtttagta tgcgtatatg agaggttgta
     4561 agcaaagcgt tgtggtctga gcacttaagg aggaatctgt gaaaatagct tttcatattt
     4621 ttgcagattt tgacttaatg ccgaaggtca gctttgcgat taccgtttat cgagaggctg
     4681 taaaagcacc gtgtagctcc gatgacaata agcaagcatt tttagagcta ggtttccttg
     4741 attctaaata tgcggcttat ttcaatgagt tggtgcttga acgcgatttt aagaaggtcg
     4801 aaaacaggca cgacttttcg acgtaaggag aaagaatttc aatgatagaa acaataaatc
     4861 ccgcagaaaa caaggtttct gcgtacattc atatcccatt ttgcgagcat atctgctatt
     4921 actgtgactt taataaagtt tttttagaag ggcagccagt agatgagtat gtggaaatgc
     4981 tgctgaatga aatgagatta atgctggaaa gtcatcctac agcgaatttg gaaacacttt
     5041 atgtaggagg cggaacacca acttctttat ctgctaaaca acttgatcga ctattatcag
     5101 gtgctagaga gatcctgcct tttcaaaatg gtaatgagtt tactgtagaa gcgaatcctg
     5161 gtgatttgac ccgtgaaaaa cttcaagtga tgaaaaacta tggcgtcaat cgtttatcca
     5221 tgggagtgca gacatttgac aatcgcttat tgaaaaaaat tggacgtaaa catactgcag
     5281 aagatgtgta tcaaacaatg caatttttag aagcagaaaa ttttaccaat gtaagcatcg
     5341 atttgattta tgcattaccg ggacaaacac tagaaggaaa tcgggatact ttggaaaaag
     5401 ctttggcttt ggatctgcct cactattctt tgtactcatt gatcctcgaa aacaaaacaa
     5461 tgttcatgaa ctgggtacga caaggaagac tcgaactgcc tgatcaggaa acggaaacca
     5521 gaatgtttga agagacgatt gaagcaatgg aaaagcatgg acgtcaccaa tatgaaataa
     5581 gtaactttgg gctctccgga catgaatctc aacataacct catgtactgg aacaatgacc
     5641 actattttgg gtttggagct ggtgctagcg gttatcttgg agatattcgt taccgcaaca
     5701 aaggaccaat ccaacactat ttacgcccat tacgagcaaa tgaacgcccg gtattggaag
     5761 aagaaaaatt gacacgaaaa aatcaaatag aagaagaaat gttcttagga ctaagaaaaa
     5821 aaacagggat tactaaaaaa catttctatg atcgctatca aaaaacaatt gaatcggtgt
     5881 acggtgatgt gctaagggaa ttagagcaag aagggttact tgttaatgaa gaagaccgtg
     5941 tttatttgac accaagaggg acttttttgg ggaacaatgt gtttgagcgc tttttatttg
     6001 aaaaatagtt tgaagaatat ttgtttttta tcgaaacttg aaaagaaaaa gtttagaaag
     6061 tttgacaatg aggtattgaa gtattgatat aactggattt acatcaatta tttagaatta
     6121 gcactcttgt gtttagagtg ctaaaaaatg gagtctttct cttgacagga gaagggcttc
     6181 ttgctatatt tataagtgaa ttagcactta gaatatatga gtgctaatga gaggtgagga
     6241 agatgttaac aaagcgacaa aacgacattt tacgtctgat catccaacac tatacgagta
     6301 gtggcgtgcc cgttggttcc aaaacattaa tgtctgaggg agtcaaggcc agctcagcaa
     6361 cgattcgcaa tgatatgaaa gcattagaag atgaaggatt gctgttgaag actcactctt
     6421 cttctgggcg gattccatct gctttaggct atcgttatta cgtggatcat ttgttaaaac
     6481 ctgctcgcgt agcagaagat gatttgctgc tgattcgtca gtctctaggc agagaatttc
     6541 atgaaatcaa tgagattatt aagcaatctg ctgagatatt atctgagctg acaagctata
     6601 cgactttttc attgggacct gaaataaaag accgccgact gactggcttt cgtatcgtac
     6661 ccttgaataa tcgacagatt attgctatcg tcgtaacaga tatggggaat gtagaaagcc
     6721 aagtgttcac tcttcccgaa tatatgggaa gtcaggaact agaaaaaatg gtacagattg
     6781 taaacgaccg tctagtcgga gatcctctga tgacggtgta ccataaactt cgtacggaga
     6841 ttccgatgat tcttcataaa tatttccaga cgacagaagg gatgtctcag ttatttgaca
     6901 ccgttcttgg tcatgttttt gaagaaaagg tttatgtcag tggacaaatg aatctcttgg
     6961 attatgagcc ccgtcaagat cttgatcagt tcaaatcgat gttttctttt atgaaagact
     7021 cagacggact tacacaaatg atcactccga tggatagcaa tattcatatc aagattggtg
     7081 ctgaattagg caatgattta ttgcagaata tgagtatgat tcaagcaagt tacgagatag
     7141 atggtcatgg aagaggtaca atagccttgt tagggcctac tagtatgcct tattctcgga
     7201 tgttcggtct agtcgatgtt tatcgaagag aactagcagc taaacttgct gattactacc
     7261 gctcgcttga cttgtcaaac tcgtaatgtg aataacgaag ctatttgcag ctgatcgttc
     7321 tctctttctt agtaagagca atcaaacagc tgactgaata atgaaaggaa gactgtatcg
     7381 tgaaagaaaa tcaagaagaa ctagataaag aaatgacaga tgctcagcca gaaccggaaa
     7441 tcgatgtcga agctgcagaa gattcgggta tttcagaagc tgaagcggaa gaattcgaaa
     7501 cagcgaaatt aaaagctgag ttagaagaaa tggaagataa atacttgcgg gctcgtgctg
     7561 aaatcgcaaa tatggcaaat cgaggaaaaa atgagcgtga gcaattacaa aaataccgtt
     7621 cacaagattt agccaaaaaa ttacttccat caattgacaa cctggaacgt gcattagcca
     7681 cagaagtgtc agatgatcaa ggggcaggtt tgaaaaaagg cgtggaaatg gtcttggaaa
     7741 gtttgcgtaa cgcactggaa gaagaaggaa ttgaaaaaat ccctgctaaa ggagaagcct
     7801 ttgatcctaa tcttcatcaa gcagtacaaa ctgttccagc gactgaagat acaccagccg
     7861 atacgattgt agaagtacta caagaaggct acaaattaca tgatcgcgtg ttaagaccaa
     7921 ctatggtcat tgttgcacaa taattgtgcg gatataaaac ttacactttc aattaaagaa
     7981 ggagacgtta attatgagta aaattatcgg tattgactta ggtacgacta actctgcagt
     8041 agcagtattg gaaggaaacg aagcaaaaat catcgctaac ccagaaggaa accgtacaac
     8101 accttctgtt gtatcattca aaaatggaga aatccaagta ggggaagttg caaaacgtca
     8161 agctgtgaca aacccaaata cgatttcatc aatcaaacgc catatcggtg aagcaggtta
     8221 caaagtagat gtagaaggta aatcatatac accacaagaa atctcagcaa tgatccttca
     8281 atatatcaaa ggattcgcag aagactattt aggtgaaaaa gtcgaaaaag cagttatcac
     8341 tgtgccagct tactttaatg atgcacaacg tcaagcaaca aaagatgctg gtaagatcgc
     8401 tggtctagaa gtagaacgta tcgtcaatga accaactgca gctgctcttg cctatggttt
     8461 agataaaaca gatcgcgacg aaaaaatctt agtattcgac cttggtggcg gtacttttga
     8521 cgtatctatc cttgaactag gagacggtgt ctttgatgta ttgtcaacag ctggcgacaa
     8581 ccatctaggt ggggacgact ttgataacaa aatcatcgac tacatggtag cagaattcaa
     8641 aaaagaaaac agcattgact tgtctcaaga caaaatggct ttacaacgct tgaaagacgc
     8701 tgctgaaaaa gcgaaaaaag atttatctgg cgtatcaagc acacaaatca gcttgccatt
     8761 tatcacagca ggagaatctg gaccattgca tttggaaatg acattgacac gtgcaaaatt
     8821 tgatgaatta acagctgact tagtagaacg tacaaaagta ccagtacgtc aagcgttaaa
     8881 agatgcaggt ctgtcacaat cagaaatcga cgaagttatt ttagtcggtg gatctactcg
     8941 tatccctgca gttgtagaag cagtacgtaa agaaacagga aaagaaccaa acaaatcagt
     9001 taaccctgat gaagtagttg ctatgggtgc tgctatccaa ggtggcgtga tcactggtga
     9061 tgtgaaagat gttgttttac ttgacgttac accattgtca ttaggtatcg aaacaatggg
     9121 tggagtgttc actaaattga ttgaccgcaa tacaacgatt ccaacaagta aatcacaagt
     9181 attctcaact gcggcagata atcaaccagc agtagacatc catgtattac aaggtgaacg
     9241 cccaatggct gctgacaaca aaactctagg aagattccaa ttgacagata ttccagcagc
     9301 tcctcgtggt attccacaaa tcgaagtaac attcgatatt gataaaaacg ggattgtaaa
     9361 tgtatctgcg aaagatctag gtacacaaaa agaacaaaaa atcacgatca aatcttcttc
     9421 aggtctaaca gacgaagaaa tcgaacgtat ggtgaaagat gcagaagcaa acgctgaagc
     9481 agataaagct cgtaaagagg aagttgacct acgtaatgat gtagacgcat tgctattctc
     9541 tgtggacaaa acattgaaag aattagaagg caaagtggac gaagaagaag tgaaaaaagc
     9601 agaaacagct cgtgacgaat taaaagccgc tgttgaagca aacaacatcg aggaaatgaa
     9661 aactaaacgc gatgcattga acgaaatcgt tcaaaacttg actgttaaac tatacgaaca
     9721 agctgcacaa caacaagctc aagaaaatcc agaagcagct caaggcggag cagatgatgt
     9781 cgttgatgct gattttgaag aagtcgatgg cgacgataaa taatcagttc actgaatttc
     9841 gagagaaggc caaagttgct actttggctt tttcttttga aaaatagttt gaagtatcgg
     9901 tttttgtttc aaacaaaata tgatatgata acaagcgacg tgaaaatgat gagaaccaat
     9961 aggaggaaag cctatggcaa caaaacgtga ttattatgaa gtcttagggc tctcaaaagg
    10021 agcatcagaa gatgaaataa agaaagctta tcgtaagctt tcgaaaaaat accatcctga
    10081 catcaataaa gaacctgatg cggaagaaaa attcaaagaa gtatcagaag catatgagat
    10141 tttaagtgac ccgcaaaaac gcgctgcata cgaccaatac gggcatgcag gcacagatcc
    10201 aaattacggt gctggtggag ctggtggcgg atttggtgga ttcggcggct tctctggtgg
    10261 cggcttcggt ggttttgaag atatcttcga ctctttcttt ggaggaggcg gtcggtcagt
    10321 cgatccaaat gcgcctcgtc aaggtgctga tttgcaatat acggtcgact tgtcttttga
    10381 agaagcgatt tttggtgtag aaaaagaaat caagtataat cgggaagaaa tctgtcatac
    10441 ttgtggtgga aacggtgcaa aaccaggcac acaaccaact acttgtcata aatgtcatgg
    10501 tgcaggtacg atcaatgtag aacgccaaac gccacttgga cgagtgatga gccgtcaaac
    10561 ttgtgatgtt tgtcatggca cagggaaaga aatcaaagaa ccttgtccaa cctgtcatgg
    10621 ctcaggacat gagaagaaag cacattcagt taaagtgaat gtaccagctg gtgtagaaga
    10681 aggacaacaa atgcgtttag ctggtcaagg agaagctgga gaaaacggcg gaccatttgg
    10741 tgatttgtat gtggtttttc gtgtagaaga aagtgatatc tttgatcgtg aaggttctga
    10801 aatttactat gaattgcctt taagttttgt acaagcagct ttgggggatg aagtccaagt
    10861 accaactgtc catggagatg tgaaattgaa gatccctgct ggaacacaga caggtacgaa
    10921 cttccgttta cgtggaaaag gcgctccgaa gttacgtggt ggtgcaacag gcgaccaaca
    10981 tgtcaaagta aaactgatca caccgaaaaa tctaaatgaa caacaaagag aagcattacg
    11041 tgcattcgct gaagctggcg gcatcaaagt tgaagaacag caagaagacg gtttcttcga
    11101 taagtttaaa gatgcttttg gcgggaaaaa gcggaagtag tttttctagt aaaaatataa
    11161 agtagacttg cccaaatttt ctatttctag taaaaacgct aagtcattaa taattaaaaa
    11221 ccagacgaac aatcatacgt tcgcctggtt tttttgtagt tgtcatgttt gtaccaacaa
    11281 aaaagccagc tttagaataa gataatcatc atagtttctg aaggtggtat cgactatgta
    11341 cttgatatcg tcaatgaacc gatggtactt tcttatgcga tggaacaggt agagcgattg
    11401 cttgtttgtg caggagaaca agcgatgcgg ctggtacagt taagtgaaaa aacaagaaac
    11461 taaaaatgcc cggtcgtcat gaagcgaccg g
//
LOCUS       DACIRY010000084        11472 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.086, whole genome shotgun sequence.
ACCESSION   DACIRY010000084 DACIRY010000000
VERSION     DACIRY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.086"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(21..2096)
                     /locus_tag="IYE22_001911"
     CDS             complement(21..2096)
                     /locus_tag="IYE22_001911"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ9507116.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2098..3015)
                     /gene="glyQ"
                     /locus_tag="IYE22_001912"
     CDS             complement(2098..3015)
                     /gene="glyQ"
                     /locus_tag="IYE22_001912"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ9507117.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3399..4211)
                     /gene="recO"
                     /locus_tag="IYE22_001913"
     CDS             complement(3399..4211)
                     /gene="recO"
                     /locus_tag="IYE22_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ9507118.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4354..5253)
                     /locus_tag="IYE22_001914"
     CDS             complement(4354..5253)
                     /locus_tag="IYE22_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ9507119.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5268..5669)
                     /locus_tag="IYE22_001915"
     CDS             complement(5268..5669)
                     /locus_tag="IYE22_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ9507120.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5647..6123)
                     /gene="ybeY"
                     /locus_tag="IYE22_001916"
     CDS             complement(5647..6123)
                     /gene="ybeY"
                     /locus_tag="IYE22_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ9507121.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6140..8332)
                     /locus_tag="IYE22_001917"
     CDS             complement(6140..8332)
                     /locus_tag="IYE22_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ9507122.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8356..9327)
                     /locus_tag="IYE22_001918"
     CDS             complement(8356..9327)
                     /locus_tag="IYE22_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ9507123.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10033..11412
                     /gene="brnQ"
                     /locus_tag="IYE22_001919"
     CDS             10033..11412
                     /gene="brnQ"
                     /locus_tag="IYE22_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ9507124.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3095 a         2413 c         2113 g         3851 t
ORIGIN      
        1 tctgtttttc aaacaaaaga ctattttgtg atgatttgat ccaagcttgc taatgacaaa
       61 gccatggcag ttatttgact aagttgtgtt agtcggttct tacgtactgc tggttcatca
      121 gccatgacca tcgtttgatc aaaataattt tcgatcagtg gacgaagagc gacaagctgg
      181 tcgtagtttt cgtggatcgt ttggtcagca aaacttgctt tcattgcttc tactgctttg
      241 tgtaattctt tctctgcgtc gttttcgaac agctcagggt ctacttctga ttgtttttca
      301 agctgcgctt ttttcgctaa gttcacgaca cgtgtcaatg cttccattga aggtttgaag
      361 tcttcatctg caagatgttg tttgaacaat tgtgctgctg caaatacttt tgtcagatct
      421 ttttgttctg cattcaaaac agcttcgatg acatcatgac ggatattctt cgttgtcaat
      481 aattggcgca gacggccttt gataaagtcg atcacctgtt gttgtcccgc tgacaagcca
      541 atgccaaaac gatccgtatc tttactgata acttcactaa tcgtttcttg cagaacagac
      601 aaaggaaagg cccatttttt cgattcgatg attcgaacga caccatatgc ttgacggcgc
      661 aaagcatatg gatcgtttga gccacttggg atcatcccaa ctgcaaagaa agaaaagata
      721 ctatctaatt tatcagcaat cgctaaaaca gaacctactg tagtctcagg taaatctcct
      781 tcactggata taggcatgta atgttcacgg atcgctgttg ctacggctgg tttctcgcct
      841 ttaagcagag catatttttc tcccatgatc ccttgtagtt cagggaattc tcctaccatg
      901 ttggttacta gatcaaattt atagatttca gcagcgcgtt gcagatccat caattcttcc
      961 tcagatagtc ctacttgttc gcctatgatg cgagcgatga cttgtacccg ttgcattttc
     1021 tcatagatgc taccgatttt ttcatggaaa gtaacagttt ttaatttttc aacgtattgg
     1081 tcgattgaag ctttcttgtc ttcattataa aagaattccg catcttctaa acgagcagtc
     1141 aatacttttt gattgcctcg taccacattt tccaatttta cccgatcgcc attacgagca
     1201 gaaataaaat gaggaagcaa cataccttga tcgttccgta cttcgaaata acgttgatgt
     1261 tctttcatag aagtgactag tacttcttca ggaacagata gatatttttc ttcgaagtcg
     1321 ccagaaaaga cagttggata ttcgactaga ttgttgactt cttctagtaa atcttcatct
     1381 agatcaatga tccagtgatt tttttctgct aattcgtttg cttgtgtgat gatcatttct
     1441 tttctttttt gcggtgaagc aataacaaat tgttcattca atttttcttc gtattctttt
     1501 gcatgcgtaa atgtcacatc ttcgcctaag aaacgatgac ctcttgaaac tctgtctgtt
     1561 ttgacatcca agatctcaaa cgggattatt tcgtcatcta acaatgccac gatccagtga
     1621 atagggcgaa tatattcaaa atcgtattct gcccagtgca tcgttactgg gaaagtgagg
     1681 cttttaatga catctgctaa acctgtcaat acttcttttg cttctttgcc gtgagtgtat
     1741 ttcgttacat agacatattc cacgcctttt aattctctaa aagtgatagc atctgtcgtc
     1801 agaccttgtc cgcggacaaa tccttcagct gctttcgtcc agtttccatc tgcgtccaaa
     1861 gcaatttttt tggctggtcc tttgacttct tcttcgctgt ctgcttgacg ttctggaatg
     1921 tttgttactt tgattgctaa gcggcgcggt gtagaaaacg tttcgatcgt atcataagtc
     1981 aattggtttt catctaaaaa ttttgctgtt ttttcttcta attgtttcat gcttggtgtg
     2041 actacatgag caggcatttc ttccagcccg atttcaagta ataagtcttt tgccatctta
     2101 ttcagcctcc ttcacgtttt ctggatttgc tgtgttttta ttcaataaag ggaatcctaa
     2161 tttttcgcgt tctgccacaa agattttggc tacagcacga gccatgttgc ggatacggga
     2221 aagataacct gcacgctctg ttacggatac tgcaccgcga gcatctaata aattgaatgt
     2281 atggctacat ttcaaaatat aatcatacgc tggatgaaca agattttcat cgatacagcg
     2341 tttagcttct ttttcaaaac gatcaaaatt gcttagtagc aggtcttggt cgcttacttc
     2401 aaaagaatat ttagaatgtt catattctgg ttgtttgaag atctcaccat atttcacgcc
     2461 atcagcccat tccagatcat aaacactttc aacttcttgg atataagaag caagacgttc
     2521 tagtccataa gtgatctcag aagtaactgg tttacaaggt agtcctccga cttgctggaa
     2581 gtacgtgaat tgtgtgatct ccatcccatc aagccatact tcccagccca caccggcaca
     2641 acccatcgat gggttctccc agttgtcttc aacaaagcga atatcgtgtt ctaatggatc
     2701 aattcctaaa agacgcaagc tttctaaata aagctcttgg atattttcag gtgaaggttt
     2761 cattacgact tgaaattgat gatgctgata caaacggttt gggttttctc cataacgccc
     2821 gtcagctggt cggcgggatg gctctacata agcagcattc catggttccg gtccgattgc
     2881 ccgtaagaat gtatatgggc tcatcgtacc tgctcctttt tctgtatcat aagcttgcat
     2941 cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat cttggacggt
     3001 caatttttta ctcatctgtt ttgccccttt caatttttaa gcgttgctgt caataagaat
     3061 acaggaaaga aaacgacaaa aagtccctat gtctgattac agacataggg acgaataatt
     3121 tcgcggttcc accctacttc cgaataaaga ttcggcagct tcatttatcg tactcgcaag
     3181 tgcctcttca aaaatcgttt gggtccggct tccactctcc cgaactcgct aaaccacgtg
     3241 ttttttactg atcttggtca atgtacgtat tcattttgca gttttctggt ttgtataata
     3301 gatcctctca gtgaacgatc tatttaatct acggttaata atagacggaa gtccctgtat
     3361 ttgtcaaata gttttgattt ttttgttgta tctttcattt attcttcttt ttcaggtatc
     3421 tctttttcag gcatattttt ctctggcgtc tttaatacgt gtgcccaaga cttcatttga
     3481 tcaatgaact ttttgctttt tagatgaata ccgacatatt catcatacaa ctggtcaatg
     3541 aggtcacgga ttgcctgctt cgtttcttct ttcaagtgga ttccactgat cttatcataa
     3601 gaaatagccg aaaacattcg aacgaaataa acagcgcgag gatctgcatg ataacggtgt
     3661 tcgtccattg gccagtgttt ctcgcatatc acaccgccat atttggaaga atagtcaaat
     3721 tttccttttg tttccccgca tacgacacag tgcgtccaaa ttggcgcgat tccgaagcgt
     3781 tgcagtattt gcacttcaaa gatattcgtg atgatttctg catcatctcc ttggtccatc
     3841 cgctgcagag cttgttgcgt aaattggaat aaatgcgggt catattgatg atcttcgatt
     3901 gctgcatcta ctagatttag caaatacgta ccataaccgc tcaaaaagat atcttcttgg
     3961 atgttccgaa acgcatggac gtctttgaca ctgcttaaaa acgacaaacc ttcacttcga
     4021 aaattaccga tatagactgc ttcggtaaag gggagcaaag ccggagctaa tggctgattt
     4081 tgccgatgga ttcctttgat ataaaacatc attttgccag aagattccgt aaagattttg
     4141 actaatttat ctttttcttt gtaattacgg ctagataaaa tcaagccttt cgattctaca
     4201 agctggctca tattctccct cctaaaaaat aaatctggaa aagcagattc tgccgatcca
     4261 gattatttat gtttcgatct tccgctgttt ttctcaacgt acggattttt atccagttta
     4321 tttctatacc taacctataa gaagtccaga atatcagtac tcgtcttttc ggtagccgaa
     4381 gtcttgcaga tagacttttt tgtctcgcca gtctttttgg actttgaccc aaagttctag
     4441 aaatactttg tctcctaaaa gattttcgat atcctttctt gctttcgttc ctacttgctt
     4501 cagcattttt ccgcctttgc cgataatgat tcctttttgg ctatctcttt cgacgatgat
     4561 cgtggcctgg atatggattt tatcattctc gtttcgcttc atcgaatcaa caacgactgc
     4621 gacagaatga ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga tcaattcaga
     4681 aacgatgaag tattcaggat gatcagtcac ttgatcatct ggaaaatatt gtgggccctc
     4741 tggcatttca ctgactaata catccattaa gcgttcgaca ttgtttcctt ctgtagcaga
     4801 gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa tcttcgatga ttcccagcaa
     4861 ctcgtctgga tggatcttat cgattttatt gataatcaga taaacaggag aatgcacgtt
     4921 ttttagacgt tcaataataa aatcgtctcc ccggccacgt ttttgatcag cactgatcat
     4981 aaataagact gcatccactt ctttcaacgc actgtaagct gcttctacca taaaatctcc
     5041 tagacgatgc tttggtttat ggattcctgg tgtatcgata aaaatcagct gagcttctgg
     5101 tgtagtatat actccttgga ttttatttct agttgtctgt gccttgtcac tcataatggc
     5161 aattttttga cccacgattc ggttcaataa tgtggatttt cctacattcg ggcggccgac
     5221 gattgcgaca aaccctgatt tatgttctgg catatacgtt ctccttttta attaaacaac
     5281 aaatgatata ttttagggac aaataaaatc aacccaatga tgacagcaaa acaagcggtc
     5341 actaaaactg ctccagctgc catatctttg atttttttcc caatcggatg aaaatgaaaa
     5401 tccgtaaaca tgtccactac attttcaaaa acagtattga tgatctctac tatccaaacg
     5461 agaaaaacag ctaaaagcag ccataaccac tctatccgat caagctggaa aatcacgccc
     5521 ataaataaag caaggatacc gaacgcaaca tgtttcctca tattgcgttc ttcctcaaaa
     5581 acggttttga ctccttggac agcaaattcc acagacatca ggaaatgttt gtttttctct
     5641 acgtttttat cttttgagtc cataggcatc caagatctct ttctgtaaac caaacatttc
     5701 tttctcatct tctggtgtca tatggtcata cccgttgata tgcaaaaatc catgtaccgc
     5761 taaaaagcca agttcccgat catatgaatg gccgtaagct tctgcttgtt cagcagcacg
     5821 ttcaatagag atcatgatgt ctcctagtac acgaggtaat ggattctctc catcttcaaa
     5881 aatcactggg atttcgtctt cgccttcttc ttccagagca aaactgatga cgtctgtcgg
     5941 tgcatctttt ccacggtaat cacgattgat tacttgaatt gccgcattgt ccatgaaagt
     6001 aacagacatt tctgtttcct ctggcagctt taaataagat gctgcaaatt gcagcaagtc
     6061 atcgatttct ttcattttct cttctgagac ttggtttgtt tcatccataa aggtaatatc
     6121 cattatacat cgcttctttc tattcttctt gtacttcctt tatcttctca acttctgttt
     6181 tcattttagg atatttgatc cgagaatgga aggtgctact taaaccgctg attaatgatt
     6241 tttccacttt tcggatttct ttcaaggtca gtccactatc gtctaattga ccatcggaaa
     6301 tacgctcttc gataagatta tggacaaaac tttcgatttt ttcgctcgtc ggatgatcca
     6361 ttgcccgtac tgcagcctca caactgtcag caatattgac gatccctgct tcacgacttt
     6421 gcggttttgg tccaggataa cggaattgct cttctgttac ttccggattg cgttctttcg
     6481 ctttgatgta gaaataacgc attagcgtgg ttccatgatg ctgtttgcaa atatcgatca
     6541 ccatttgcgg catattataa tcttccaata ttttggctcc atctgtcaca tgtccaaaga
     6601 taatatgttt actgtcttct ggcaataaga aattgtgcgg gttttccgct cctgaaggca
     6661 agttttcaac gaaaaagcta gcatgtttga tcttgccgat atcatgataa tagcaagcta
     6721 ctcgagttag caaagaacgt ccgccgattt ctgctacagc gttagcactt agattagcta
     6781 ccatcatact atgatgataa gtacctggcg cttcttccag caactgtttc aataaaggat
     6841 gatttggatt gctgagttca tttaatacaa ttacactgtc atcattgacc atcaattcaa
     6901 tatatgggtg tagaccgatc gtcagcaaga atgagaagag tgcaccagtg aacccgcaga
     6961 cgagcattaa ccaagtcgtt gcatcaccaa agctcattcc ttgatagacg atcaaaatga
     7021 catccatcat cacaggaaaa ataatgaccc acatggcagc agataatcct tgttctgaga
     7081 tccgtttgcg tctgacaatc gttgccatga gcccagagaa aacataagac atcaagataa
     7141 tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa aagtgcggat actgcttgga
     7201 aaataccagc catgatccct gctcttcgat tcaagaaaaa gcttaaaaca agtggtgcaa
     7261 aagcagcggg ataaaacagc gggatataag tagcttgttc cgtttggaat aattggaaaa
     7321 atttcatcag cactatgctg actgacatag ctgtcacata gaaaagcacg tattttgttc
     7381 tttctcctgc attttggaat tgaagtgaat tataaatcag tacgccgatt tgaagcaaaa
     7441 cagctaaaac catggcaatt agcggaaaaa tcgaagttgt ttgattagtc agtcctagta
     7501 attccaattt tttcatagct gaagcatcga tttgattacc ttcacgaaca atgatttctc
     7561 cttggaagat catcactggt tgtacagctt ctcgtgcgga ttgtttcaat tcttctgtct
     7621 ttttctcatt caatacgtca ttcactacga taccttgatc cactaagtag cgaatcattt
     7681 gttgttgtgc tggtgtgaca ttcaaatatt ggatttgctc ttctgcttct tgtttaaaag
     7741 cttccaattc actttcacga acttgtttgc tcatctgttc gtcaatgagc ttcagactct
     7801 catccctcac agtatgaatc tcagtttcag tcatttcaaa tatcgtcgaa taaaaattgt
     7861 tcggtagttt ttgataaaac acaacatttt ctgcattcac attttcaaaa tttttcttca
     7921 gcgcggcgat ccgctcttct gtagtaggct ttgggatatt ttcatcttcc tttgccttat
     7981 caatcttttc gtcataagat ttgttgatgg catcattcgt cttatcgatc aattcaaata
     8041 gttgcttgat ccgattgttc tggtcatctg caagatcttt ttggtaagtg tattctggag
     8101 tgactgcttc tgctgccagt tttctttttt gttcggtttc ttccgtgttc tcgatcgttt
     8161 tgttcgcacg gatactttct tctgctactt ggccttctct gaaatcattg gccttttgtt
     8221 tgacactact aaatgtaacg ccgcagataa tgataaaaaa caagaacagc aggaaaggca
     8281 atgctgcctt tccaagtttt tcttgtagtt tctgcagtgt tttattcaac atgtgcttat
     8341 cctcctaagc aaagcttatt cttgatggag atctgccttt tcataagctt tgatgatttc
     8401 ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat cgattttctt
     8461 gatttgcttt aatgttcgtt ctgcatgaac aagaccactt gtcgtccctc gaggcaagtc
     8521 tatctgactt gtatccccat tgacaatcat ttttgattga taaccgagac gggtcaagaa
     8581 cattttcatc tgagcgactg tcgtgttttg tgcttcgtcc aagataacga atgcatcatc
     8641 caatgttcgc ccccgcatgt aagctaaagg tgcgatttcg attacgcctc gttccatcaa
     8701 tcggttcgta tgatccaatc caaaaatttg gtataaagca tcataaacag gccgcagata
     8761 aggatccact ttttctttta gatccccggg aagaaagcct agattttcac ctgcttccac
     8821 agcaggccgc gtcaaaatga ttttttgcac ttcacctttt ttcaaggcag cgattgctaa
     8881 tactactgca agaaaagttt ttcctgtccc ggctggtcct acgccaaata cgatatcatg
     8941 tttggctacc gattcgacat atttcttttg gccgctgttt tttacccgta taggttttcc
     9001 attacggtct ttgacgatct cttcctcata catctcaaca aaataatcta atgtaccttt
     9061 ttgccccatt ttcaatgccg tcacaacatc gggcgtactt acgtggattc ctcgtttgat
     9121 caattcctgt aatgtccgaa tgactgaggc agccaaggaa atctgggatt gctcaccaat
     9181 gatctgaatg atttctccac gtgtatggat ggttgtttcc gttgtttcct caatcagttt
     9241 tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca tcagcagccg tcaatctaat
     9301 ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc ctttttttcg attaattctt
     9361 tcctatttta ccatactcag aagaaactat atagcaaata agaaaaggtt ctaaaagagt
     9421 tgcttaacca taagtaccaa gaaacgttaa aaaactagcc gcttccattc tctaaaggtc
     9481 tcgtttgaat aaaaaaagct gatcatgtaa gctatgcata agatagagga caagtattcc
     9541 gtctaactta gcaagtattc tctgggtatt tttcttgttt caaaaaaaga aaacaaaata
     9601 tttgaaatat tcttgcattt caaaaaaaac aggtgtatta taacgtcaac aaataaatat
     9661 acagcaaagc gtcgaggaaa acagtaggct attcagtgat ttattcccag tgagtcatgt
     9721 aagtgagaaa tgacaataaa tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac
     9781 gtagatcatt gtggctgcct ccattatcag gctagcggat aactttatcg aaaagcgtcc
     9841 gtgaatgaag aagctttttt aagcgaaaaa aggtggtacc gttctagaat ctagagccct
     9901 tttcccaagt ggaaaagggc tttttttata ttttaaggtg gtggttttca tggaatctga
     9961 tattagtggt atttctagtt tctgcaacgt ttattttgtt actcaaaatg ataattattg
    10021 gaggaaaaat ttatggaaga aacaaaaact tattcatgga agcaattatt gatggtcagt
    10081 tcacttatct tcgggatgtt cttcgggtca ggtaatctga tctttcctgt acacttaggt
    10141 caaatggctg gatcaaactg gatcagcgct ggtgtaggat ttgccatctc aggcgctctt
    10201 ttcccattat tagctatctt agctgtagta gtgacaaaaa gtgatggtct gtatgactta
    10261 gctaaacctg tcggaaaatg gtatgctgcc ttgttcttga tccttgttca tttaacgatc
    10321 ggaccattct ttggtacacc gcggacagca gcaactgctt ttgaaatggc tgctaaaccc
    10381 tttttgcctg aaaaatatga ttcattaggt atgtttttat tctctgctct tttcttttta
    10441 cttgcttatt tccttactgt acatccaaca cgtttggtca aatatgtcgg aaagtattta
    10501 aactccttgt ttcttgcatt attagctatc atttttgtcg tttctttatt ccaacccatg
    10561 gggaatctaa accaatctgc tgcagattct tatcagacga acgctttgac aaatggcgtt
    10621 ttagaaggat acaacacggt tgatgcagta gctttacttg ctttaagtgt cacttttgtc
    10681 catgcagttc gtgatcttgg ttttaaagat aaaaaagtca cagaattaac agcaaaagct
    10741 ggtagcctga gcgttgcgtt agaagtcatg atttattttg gtctggtcct tctcggtgcg
    10801 atgagtttga ataagatgtc tttatcagaa aatggcggaa ctgcactttc tcagatcact
    10861 aatttttatt taggtaactt cggtgctagc ttcttagggg taatggtcac attaggtgtc
    10921 ttcactactg ctatgggact cgttgtttct tttgcacaag atttccataa actgttccct
    10981 aaagtcagtt atatgacatg gctgcggtta acgacttttg tctcttttgt cgttgcgaat
    11041 gccggattgg ataatatcat ccaatggtct ttaccagtat tgatgctttt gtatccattg
    11101 tcacttgcgt taatcttact ttccttaaca gcaaaattct tccaaaagag tccatttgtc
    11161 tatcaagtaa cgatgttctt ttctgccgtt ccagccgtat tggatatgtt ggcaaattcg
    11221 cctgcattag tgagtcagca aactgtcgta gcaagcttac taggatttta tcatcattat
    11281 gttccttttg ctgcattagg tcttggctgg gtcgtcccaa cattgcttgg ttacgctggc
    11341 agcttgctct tttattatgt atatcgcgta tcaggatata aacaagaagc aagtgagtta
    11401 ccagaagaat aaccaaatca aacacaattt aacataagag atattaaaca ggaaaaaaac
    11461 agctagaaga aa
//
LOCUS       DACIRY010000085        11389 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.087, whole genome shotgun sequence.
ACCESSION   DACIRY010000085 DACIRY010000000
VERSION     DACIRY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.087"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            134..409
                     /locus_tag="IYE22_001920"
     CDS             134..409
                     /locus_tag="IYE22_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ9507125.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="IYE22_001921"
     CDS             415..1182
                     /locus_tag="IYE22_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ9507126.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="IYE22_001922"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="IYE22_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ9507127.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3439..5211
                     /gene="recQ"
                     /locus_tag="IYE22_001923"
     CDS             3439..5211
                     /gene="recQ"
                     /locus_tag="IYE22_001923"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ9507128.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5325..5627
                     /locus_tag="IYE22_001924"
     CDS             5325..5627
                     /locus_tag="IYE22_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ9507129.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5630..6793
                     /locus_tag="IYE22_001925"
     CDS             5630..6793
                     /locus_tag="IYE22_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ9507130.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6819..10247
                     /locus_tag="IYE22_001926"
     CDS             6819..10247
                     /locus_tag="IYE22_001926"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ9507131.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10268..>11389
                     /locus_tag="IYE22_001927"
     CDS             10268..>11389
                     /locus_tag="IYE22_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507132.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
BASE COUNT         3786 a         1967 c         2485 g         3151 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaact tatttctaga gagtttttgt ttcataataa
     3361 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
     3421 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggcta tgatgagttt
     3481 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
     3541 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
     3601 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
     3661 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
     3721 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
     3781 atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
     3841 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
     3901 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
     3961 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
     4021 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
     4081 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
     4141 agaaaagaag tagaccgtct ctatcatttg cttagaaaat tcgatttttc cgtgggtaga
     4201 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
     4261 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
     4321 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
     4381 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
     4441 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
     4501 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
     4561 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
     4621 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
     4681 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
     4741 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
     4801 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
     4861 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
     4921 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
     4981 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
     5041 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
     5101 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
     5161 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
     5221 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
     5281 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
     5341 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
     5401 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
     5461 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
     5521 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
     5581 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
     5641 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
     5701 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
     5761 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
     5821 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
     5881 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
     5941 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
     6001 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
     6061 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
     6121 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
     6181 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
     6241 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
     6301 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
     6361 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
     6421 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
     6481 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
     6541 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
     6601 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag ggattactgg
     6661 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
     6721 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
     6781 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
     6841 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
     6901 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
     6961 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
     7021 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
     7081 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
     7141 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
     7201 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
     7261 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
     7321 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
     7381 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
     7441 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
     7501 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
     7561 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
     7621 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
     7681 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
     7741 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
     7801 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
     7861 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
     7921 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
     7981 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
     8041 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
     8101 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
     8161 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
     8221 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
     8281 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
     8341 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
     8401 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
     8461 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
     8521 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
     8581 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
     8641 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
     8701 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
     8761 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
     8821 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
     8881 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
     8941 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
     9001 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
     9061 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
     9121 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
     9181 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
     9241 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
     9301 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
     9361 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
     9421 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
     9481 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
     9541 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
     9601 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
     9661 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
     9721 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
     9781 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
     9841 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
     9901 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
     9961 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    10021 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    10081 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    10141 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    10201 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    10261 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    10321 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    10381 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    10441 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    10501 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    10561 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    10621 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    10681 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    10741 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    10801 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    10861 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    10921 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    10981 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    11041 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    11101 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    11161 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    11221 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    11281 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    11341 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgt
//
LOCUS       DACIRY010000086        11368 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.088, whole genome shotgun sequence.
ACCESSION   DACIRY010000086 DACIRY010000000
VERSION     DACIRY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.088"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(58..285)
                     /locus_tag="IYE22_001928"
     CDS             complement(58..285)
                     /locus_tag="IYE22_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9507133.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(388..2976)
                     /locus_tag="IYE22_001929"
     CDS             complement(388..2976)
                     /locus_tag="IYE22_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ9507134.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(2996..3655)
                     /locus_tag="IYE22_001930"
     CDS             complement(2996..3655)
                     /locus_tag="IYE22_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ9507135.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="IYE22_001931"
     CDS             complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="IYE22_001931"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ9507136.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(4428..5294)
                     /locus_tag="IYE22_001932"
     CDS             complement(4428..5294)
                     /locus_tag="IYE22_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ9507137.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            5403..6044
                     /locus_tag="IYE22_001933"
     CDS             5403..6044
                     /locus_tag="IYE22_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ9507138.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="IYE22_001934"
     CDS             complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="IYE22_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ9507139.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(7485..8384)
                     /locus_tag="IYE22_001935"
     CDS             complement(7485..8384)
                     /locus_tag="IYE22_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ9507140.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(8386..9183)
                     /locus_tag="IYE22_001936"
     CDS             complement(8386..9183)
                     /locus_tag="IYE22_001936"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ9507141.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(9161..9865)
                     /locus_tag="IYE22_001937"
     CDS             complement(9161..9865)
                     /locus_tag="IYE22_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ9507142.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            9996..10571
                     /locus_tag="IYE22_001938"
     CDS             9996..10571
                     /locus_tag="IYE22_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ9507143.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            10689..11366
                     /locus_tag="IYE22_001939"
     CDS             10689..11366
                     /locus_tag="IYE22_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ9507144.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         3234 a         2471 c         2040 g         3623 t
ORIGIN      
        1 ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca
       61 aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg
      121 cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg
      181 actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga
      241 ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc
      301 ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc
      361 atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg
      421 agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt
      481 aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa
      541 tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc
      601 actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc
      661 atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag
      721 agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc
      781 tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc
      841 cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg
      901 gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca
      961 ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa
     1021 ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat
     1081 ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt
     1141 gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg
     1201 attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat
     1261 tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat
     1321 tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg
     1381 attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat
     1441 actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat
     1501 ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc
     1561 tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt
     1621 atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac
     1681 tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag
     1741 acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg
     1801 aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag
     1861 attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa
     1921 tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc
     1981 gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc
     2041 ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa
     2101 actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat
     2161 aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg
     2221 gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg
     2281 ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc
     2341 gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag
     2401 ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact
     2461 gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc
     2521 gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat
     2581 ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc
     2641 tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc
     2701 tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct
     2761 tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat
     2821 atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact
     2881 gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac
     2941 tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata
     3001 gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt
     3061 catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca
     3121 atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag
     3181 ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat
     3241 ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag
     3301 aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt
     3361 acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg
     3421 tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg
     3481 ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa
     3541 atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa
     3601 aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca
     3661 gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat
     3721 tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca
     3781 ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca ttcataggga
     3841 tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt
     3901 ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat
     3961 gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta
     4021 aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat
     4081 gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg
     4141 cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat
     4201 gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt
     4261 tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga
     4321 agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac
     4381 ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta
     4441 gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa
     4501 ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg
     4561 gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt
     4621 tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt
     4681 ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc
     4741 accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa
     4801 ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa
     4861 tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt
     4921 gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag
     4981 ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata
     5041 gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt
     5101 ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa
     5161 aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct
     5221 tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca
     5281 atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt
     5341 ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt
     5401 agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca attgctagag
     5461 gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg
     5521 gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta
     5581 ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg
     5641 atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt
     5701 caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga
     5761 tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt
     5821 ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta
     5881 ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca
     5941 ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg
     6001 taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa
     6061 acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc
     6121 cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt
     6181 gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat
     6241 taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa
     6301 ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac
     6361 agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc
     6421 tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt
     6481 accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa
     6541 gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca gagtgatcag
     6601 ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac
     6661 cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc
     6721 gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc
     6781 ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac
     6841 tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag
     6901 agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc
     6961 agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac
     7021 gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag
     7081 ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt
     7141 tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc
     7201 agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc
     7261 aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct
     7321 gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa
     7381 actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct
     7441 ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt
     7501 ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta
     7561 aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc
     7621 attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact
     7681 cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt
     7741 cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc
     7801 aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct
     7861 gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc
     7921 atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc
     7981 cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa
     8041 gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg
     8101 cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt
     8161 tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca
     8221 tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct
     8281 tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt
     8341 acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat
     8401 aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg
     8461 ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc
     8521 aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt catcatgggc
     8581 aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc
     8641 caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc
     8701 tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc
     8761 gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt
     8821 cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa
     8881 gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc
     8941 gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac
     9001 atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac
     9061 tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc
     9121 gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt
     9181 catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat
     9241 ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac
     9301 tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca
     9361 tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga
     9421 aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca
     9481 gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct tttttatttg
     9541 tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa
     9601 cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt
     9661 cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga
     9721 ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact
     9781 gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta
     9841 aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt
     9901 attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga
     9961 aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat
    10021 ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat caattaacag
    10081 ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga
    10141 aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa
    10201 cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag
    10261 catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt
    10321 tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat
    10381 tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt
    10441 ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt
    10501 taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag
    10561 ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc
    10621 tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct attttggggg
    10681 agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa
    10741 tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga
    10801 tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa
    10861 aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg
    10921 cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga
    10981 tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat
    11041 ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact
    11101 tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac
    11161 acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag
    11221 taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat
    11281 atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta
    11341 gccatttaca cttgctttct aattaaaa
//
LOCUS       DACIRY010000087        11335 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.089, whole genome shotgun sequence.
ACCESSION   DACIRY010000087 DACIRY010000000
VERSION     DACIRY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.089"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..403
                     /locus_tag="IYE22_001940"
     CDS             <1..403
                     /locus_tag="IYE22_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9507145.1"
                     /translation="ISSHRGLPQAKLFTDLPKLTLGDSFYIEINGRTLAYQVDQIKTV
                     EPTDTKDLRIEKGQDFVTLLTCTPYMVNSHRLLVRGHRVPYHAKEVQKEVQKVSENKR
                     FFLYGLLAACILIFLLLIYLFRQSFKIRNRS"
     gene            complement(555..1430)
                     /locus_tag="IYE22_001941"
     CDS             complement(555..1430)
                     /locus_tag="IYE22_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9507146.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            1637..2668
                     /locus_tag="IYE22_001942"
     CDS             1637..2668
                     /locus_tag="IYE22_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9507147.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFPLAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            2757..3062
                     /locus_tag="IYE22_001943"
     CDS             2757..3062
                     /locus_tag="IYE22_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ9507148.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            3241..5256
                     /locus_tag="IYE22_001944"
     CDS             3241..5256
                     /locus_tag="IYE22_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ9507149.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            5258..5542
                     /locus_tag="IYE22_001945"
     CDS             5258..5542
                     /locus_tag="IYE22_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9507150.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            5568..6941
                     /locus_tag="IYE22_001946"
     CDS             5568..6941
                     /locus_tag="IYE22_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ9507151.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7024..9039)
                     /locus_tag="IYE22_001947"
     CDS             complement(7024..9039)
                     /locus_tag="IYE22_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ9507152.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(9061..10275)
                     /locus_tag="IYE22_001948"
     CDS             complement(9061..10275)
                     /locus_tag="IYE22_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9507153.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            10496..>11335
                     /locus_tag="IYE22_001949"
     CDS             10496..>11335
                     /locus_tag="IYE22_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9507154.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSL"
BASE COUNT         3656 a         2102 c         2125 g         3452 t
ORIGIN      
        1 tatttccagc catcgaggac tgcctcaagc aaagttgttc acagacttac cgaaactgac
       61 tttaggtgat tctttctata tcgaaatcaa tggacggaca ctcgcttatc aagtcgatca
      121 gatcaagacg gtagaaccga ctgatacaaa agatctgaga atcgaaaaag ggcaggattt
      181 cgtgacactc ctaacttgta caccttatat ggtcaacagt catcgcttac ttgtcagagg
      241 gcaccgggtt ccttatcatg caaaagaagt tcaaaaagaa gtacagaaag tatccgagaa
      301 caaacgattt ttcctgtatg gattgttagc tgcatgtata ctgattttcc tccttcttat
      361 atatcttttt cgtcaatcgt tcaaaataag aaacagaagt tagctattcc tcatttctat
      421 aatatatcta ttcgaatcgt tccgtaaaca aacaggctga ggtttgaaaa agaaaacttc
      481 agcctgtttt tatgatgata tcaaagagag cctatcaaac actatctttc ttattactaa
      541 atgaggatct tttcttaagt tttccgatat tctttaagaa ggcctttttg catcaattct
      601 tgtttgtttt tcaagttgcg gatattctct tcaaaatttt gagaatatat cactgagaat
      661 aacgtattta atacgtaatc gaaggcaatt tgagaggaga aagtcgctac tttagcaaaa
      721 tcatattcct cctgcgatat aacaagacac atatcagcct gtttggccat ttctgaatgc
      781 tgatttccgg taatcagcaa ggtaggggcc tctacatgct tcaaataggt cattattcgt
      841 tcataatgat ggatgcgacc gctgtagcta ataaaaatgg cgcaatcctc gctagtcaaa
      901 ttggctgcat tccaagaaga atcactgtat tcttcagcca tgatcaaaaa cttattcaac
      961 ttgaccattt tattctggaa tttccttgcc gtgatttgag aatcaccttt tgcaaaaaga
     1021 aagattcgct gtgccttgtt caatacttta ccgatttgat ctaaatcttc atgttcaatc
     1081 tgcaattgtg ctttttttat tgactggatc gataaatccg ccattttctt tgcaatttgc
     1141 tgagtgctat cttcacttga aaaaggaaaa ttaggatcaa tcgtttgatt gaactgagca
     1201 atcaattgat gaacttcccg ccccagctcg aaacggaatt ctttaaatcc gctgaatccc
     1261 atttttttgc ataaacgaat gatagctgaa tgggaagtat acgtagcttt tgctaattct
     1321 tcaatcgtca tgtagactgc agcttcgata ttcctacgga tgtaatcgac aatccgtttc
     1381 tctgtcgtgg taaacaactc ttgttttgtt agtttttctt caataagcat gatctgacct
     1441 ccaaattcta tcttactgat aaacacttgg ttccgctact atctttttga tttttatcct
     1501 tactttgaac attaatttca ccaaaccgaa tgataacaag tgtttttctg aactttttta
     1561 ccagaatagc tattataaat actttctgcg cttttctagg atatactgaa aacatcaata
     1621 aaaggaggca ccactcatgc ttacaatcgc ttatcttggc aatggaaaga gtacaaatcg
     1681 ctatcatttg cctttttcaa gaaaacttac gaacaaaatc aatatcaaaa aaattttttc
     1741 cccatctgaa ccagtgtgga aaacattcga agacatcgaa tatactgatc agctgtctga
     1801 tctttgggat gatcctgaga tccaattagt tgtcatcact acacctagtc aatttcatta
     1861 tgaatatgga aaaatggcac tcgaacatgg taagaatgta ctagtagaga aaccttttgc
     1921 agaaacgtca aaggaagcag aagagttatt tccattagca aaagaaaaag gtctatttat
     1981 ccaatgttac caaaacagac ggttcgattc tgacttcttg acagtccaaa aagtaatcga
     2041 aagcggaaaa ttaggggata ttttagaagt agaaatgcat tatgattatt tcagaccgga
     2101 agtaccagaa aatacgcatg aattttctac tgcgaacagc tatttatatg gacatgcttg
     2161 tcatacagta gatcaagttc tttcttattt cggcgaacca gatgatatca gctatgatgt
     2221 acgtcaactt ttagggaaag gacgaatgaa tgattacttc gatctggatt ttttctatgg
     2281 aaatatgaaa atttcgataa aatctagtta tttccgcatc aaagaacgtc caagtttcgt
     2341 cgtttacgga aaaaaaggga tgttcgtgaa acaaacaaaa gatcgacaag aagaacatct
     2401 gaaaatgttt tacatgcctg atcatgaaga ctttggtatc gatttgcccg aacattatgg
     2461 tgtattgacc tatatagatg aacaagggag ttatcatgaa gaaaaagtaa tttccgaggt
     2521 tggtgattac agccgtgtct acgaagggat ttacgagaca ttagttaatg gcgcagaaaa
     2581 agtcatcaaa gatgaagaaa caatcttaca aatgaaaatt ttggaagaag gaatcaaaag
     2641 cattcaagaa tcagaaggaa aaatatgaca tatttgtaag aatcgggttt gttttttgac
     2701 gcataaactg tcttattgta cgatgaaaaa gtatagaact actaggagga accaacatga
     2761 caaattatga cggtgaaaaa cacaaacgat taagaggttc aatggcccct aaaacagaag
     2821 aagaagtggc tttagcagga actaattctg gtgaaacaga agacaaagaa gaatcattag
     2881 ctggcacaaa ctctggtgaa acagaaaata aagaaacagc tttatctggt tcaaattctg
     2941 tagaagagga aacaaagcct aatgagccca atcactttga aaaagatatc gaaaaagcgg
     3001 ttgaagatgt caaagaaaaa tttgaagaac tgacctcaaa aaataaacat gatcatcatt
     3061 gatcggacga aatcaattca taaaagcagg agacatttgg tcacctgctt tttttctgta
     3121 tccattcgat agaaatcgtt cggtacagtc gtaaaaaaaa tccaactgta agttgtacca
     3181 aacacccctc gatttaaaag cgctaccata ctagaatata agtaggaggt gaacagcgct
     3241 gtgaactaca aaattagtta tctcctgaaa acaccaagag aaatcagtct agaaaaagca
     3301 gcagagatca tcgaagtaaa tacaagcgag gcgatcaaac aattaactcc ttttgccgaa
     3361 tatgtggatg atacccgtat ccgtataaag aaattccgag caatcgactg ggcagaaaaa
     3421 tttttggata ttactcgatt tgaagctgtg tataccgaac aagagcgaag agatcttctt
     3481 tatttgatgg tttattcgac tgcaacgtct gtttctgttt ttcattttca agattttctt
     3541 caggtttcta aagggacagt cctgtcagat atcaaaaaga taagaaatga attagaagtg
     3601 gaagattgtc agctgtcgta tactaggaaa actggtttta ggctgctagg gtcagaagca
     3661 atattaagaa gagaagcaaa aaatcgcact gctcgtttga tgcagtcact tgctggaaga
     3721 tttgggctca cgtatttaac cgcaaccctt tccattgatt attacgccaa agttagagat
     3781 ttgctagata aaacgatttc actatcagat cttcgattcg caccaaatcg aatggatgaa
     3841 ttagcttatt atttgagttt gacaaccatt cgaattggaa aaacatccat ggaactggta
     3901 aaagatgctg aactgttggc ttctcttgca gtcactgaca ttagtcgaca agtattgact
     3961 ggccttggct attccgaaca tcaaaattcg gagattatat ttttctctct ttgcttagca
     4021 tcgatcgttg aaggagatgt ccaggagcct gcacttgatt ttttgctgga atgttcttca
     4081 gaagtcatcc atcgcatgga attcctgatg gccgtagaat ttgcttcgtt tcgtgaatta
     4141 ttgatgaatg tcttttatca cttggtccct gcctattttc gtatttccta ttctttttat
     4201 ttacctaatg tgatgatcga tcaaattaag catcaatatg cttccatcta cgaaatgaca
     4261 agaaaagcat tacttccgtt agaaagacga atagggaaga gcatccctga agaagagatt
     4321 ggttttttca ctatactatt tggcggagag atcagaaaag cggacgcaga agaaagaaat
     4381 agaaaaataa gagccgtgat cgtctgtcca agtggcatta gctcttcatt gatcttaaaa
     4441 agtgaacttc agcaattatt cccgatgatc ttttttacag agactaattc agctgaccgc
     4501 ttacacgagg ttgacgaaac ttcttacgat attatttttt ctactgtacc tctcacactt
     4561 actgataaga aaaagtctct ctatgttctg catccaatca tgactgactt ggaaaaaaat
     4621 cagctgatca atcaagtaca aagagattgg ctgataccag gattttcaat gccttctgcc
     4681 caagaattgt tgtcggcgct actgccttat atcgaattaa aagaaggcgt agacgaagaa
     4741 aagctctatc aagtattaaa tcgtaaaatg aacaaacttt tagaaaagaa ggaggatcaa
     4801 cgtcctatgc tatctgaatt attaacagaa gaaatgatcc aattatcgga tcaaccaaaa
     4861 aactggcaag aagcaatcac atttgccgca cagccgctat tatcagaaaa taaaatagaa
     4921 gaaagttata tcaaagctat gatcgagcgc gtggagcagt atggtgcgtt tatccatatt
     4981 ggcgatcaca tcgcattacc tcatgctcgt ccagaagatg gcgtaaacga agtagggatg
     5041 tctttgctga aacttcaaca acctgtggat ctgcttgata atccagccca tcccattagt
     5101 ttgttcatct gtttagctgc tatcgataat gaggcacatc ttcgggcttt ggcaaattta
     5161 acaaagctac tttctaataa acaaaatctt caagatttat tacacgcatc aacaaaagaa
     5221 gaaatattag cgatcataag aaaaggagac gaatgatatg aaaatttttg cagtatgtca
     5281 aagtggatta ggaacaagtt ttatggtaga gatgaacatc aaacaagcac ttgcagatct
     5341 tggagcagat gtggacgagt ttgaagtaag tcatacggat gttggaagtg tatctagcga
     5401 tatggctgat tatttcttca ttgagcattc tttgaaagat acgctaactt ctatacctga
     5461 tgaaaagctc gtacctttgc aatcgatcat tgacagcgaa gaagtaaaag aaaaagtaac
     5521 gaagatcttg tcaaaagaat aaaaatgatt caagggagga actatcaatg gataaattgt
     5581 tgaatttgtt tgtgtcaatc gtcagtcagc cctccatcct cgtttctttg attgcactgc
     5641 ttggtctcgt tcttcagaag aagaaatttt cagaagtgat ccaaggaact atcaaaacat
     5701 ttgtcggatt cctagtactg atcggcggtg ctggtttaat cagtaattca ctaacccctt
     5761 ttgcttcgat gttccaaaca gctctgcata ctcaaggagt agttcctagc aatgaagcag
     5821 tagtggcgat tgctttgcaa caatacggta cacctactgc tttgatcatg ctagtaggga
     5881 tgatcgtgaa tattttactt gctagattca caagatataa attcattttt cttacaggtc
     5941 aagcaatgct ttatgtttct tgtctaacag cagtcatttt agtaagcgcg gggtttactg
     6001 caaatttgtg gatgatctta ttaggcgggg tcttcgaagg aacactatta actattacgc
     6061 ctgctctttg tcaaccgttt atgaagaaca tcactggaaa caatcaagtt gcgttagggc
     6121 ataccggaaa tatcggttat gcttctagtg gatggatcgg aaagtttttt ggaaacaaag
     6181 accgttctac agaggacata aagattccta aaagtttcag ctttttgcga gattctactg
     6241 tatcgatcat gctcttgatg tcgatcgtat atgtcgcact tgctttgtta gctggtcctg
     6301 gatttgttga aagagaacta agcaatggag acaacgctgt aatctttgca ctgatccaag
     6361 ctggaacatt cacagctggg tttgttatcg tccttcaagg ggttcgaatg gtgttggcag
     6421 aaatcgttcc agcattccaa gggattgcta aaaaactcgt acccgattct aaaccagcat
     6481 tagatgttcc tatcgttttt ccatatgcac ctaatgctgt attaattggc ttttttgtta
     6541 gcttctttgt aggaacacta tccatgtttg ctatgatagg cttgcaaact acagttatca
     6601 ttcctggggt agtaggacat ttcttctgcg gagctgcttc tggtgtcttc ggcaactcga
     6661 ctggcggtag aagaggggcg gtattaggtt ctgctttcaa tagcttattg attagttggc
     6721 tgccattatt tatccttcca gtattaggag atcttaaaat cgcttcttct acatttgccg
     6781 atactgatta tctagtacca ggacttgtac ttgggaaact aggagaatac ggtcaaatag
     6841 ccttgacagt tggaatcatc gctttctttt tacttgtcgt attatctagc tttctttttg
     6901 ccggaaaaag aacaaacatt gtttctaaag aacagaatta agcaaacttc aaaataaaaa
     6961 ttgcttggac aatatcagaa gaagatttaa agtcttctga tatgtcgcaa gcagtttttt
     7021 gttttatgca tttatttttg aatagcttgt taagacaagt ggctgttttg ctagaagagg
     7081 ataactttta ccattcattt tcactgttcc gcctgttcta tcagaagtaa tcacgatctt
     7141 atcttttgtt ttttcaagcg aaatctttcc atctttgata ggtagcgtac aattcagtgt
     7201 ttcaaatgaa tctagattag gttgtatcat aaattcttca taacctggct ttgttggata
     7261 caaaccgata aaatagcgtc ctaaaagata gattgggctt gctccccatg catggcataa
     7321 gctttttcca taaggatctc catacatact gtacttttca tttcctgttt gagtagggtc
     7381 gtattcttcc cagaaagtta ccgcatcatg agataacatc cctccccaat aatcaagaat
     7441 cgtttggtag acgatattcg tctgtccgag tttgcataaa gcatcttgtt catagaattt
     7501 gaaatatgga gtagtaatct gtgtgatagt gtcattcaat aagacattct ttaagatcga
     7561 ttgctgttgg tctttgttag caaaatcgaa taaaacagcg aaaatattcg catgacgcgt
     7621 cacatttctt ctcccagatt catagcaatc gataaaagcg ccaagttctt catcccaaaa
     7681 gtagtcaaat aggttttgtt tcaatagttg atatttttct tgatagagat gagtatgata
     7741 attttcgttc tctccatatg tttcagctaa gatatctccg caagtgatca tggaatgata
     7801 ggctttcagt aataagactt gttctgcagc aagcgttcct tctttatcca tttcagacca
     7861 atcaataaat atccaatctt ttgctctccc ataaataaac cctaattcat tcgtttgctc
     7921 catcaagtat ctcattaatg attgcatctt agggtagacg atgcgtaaaa attcatagtc
     7981 tttcgtcatc atatagtggt tttcgatacc aatcaaccac aagatcgaat agtcaacgat
     8041 agtattcata tgctgcttga tttcattttg tcctcttaat gctaatatcg ttcgtttatt
     8101 gatttcctcg tcaaaaaaaa gatattgatt gatcatgaat gattgatacg catctccaga
     8161 ccaaatccag cgatctcgtt tgataccgtc gataaaaaac aaatcactac ataacttgaa
     8221 ggcttcttct gatactgacc agatctgatt cagtaaagaa gaatcgcttg aaaaagcagc
     8281 ttgtttcgaa agagataaaa attcatgaaa aacttgaagt tgaaccattc cagtagaaac
     8341 ctctggtata aacaaatagc gaaaagcgcg tttacgagct ttcatttttc ttgtcattcc
     8401 tatttgctta tagtagcaat aatcgatatc catagcttct acatcggact ctccataaca
     8461 tagagtgata gattcgatat ggtcatccag agatattagt tctacacagc cattcactgc
     8521 tcgcccaaaa tcgaataaga ttcctccgcc aatctccgtt tcagtgactg gatggacgca
     8581 ttctttttga taatagattt gatttggatc ttttgcgata tcgacataaa gcggactcca
     8641 gccagctgga tattcttcaa taaaattact tgctgtccag ccaatatctg attttacttc
     8701 gtctccctcg ataaacatcg ctggtagccc tgatgtatgt cctacaaaga tacgaatttt
     8761 tgctttacca gctggacaag tcagccattt tccacatgga tgtttttgtc cattaatttc
     8821 tacataccca actccttgga tcgtcacttt aaacatggaa ggttgatcaa gaaagtaata
     8881 tctcttaaac ttgacgttcc gatggcaatc atccatgtac cagtacgctg gccagtcaaa
     8941 acctcgttct tcccgttgga aattttgtag cagtccatgg cggatctcaa agtctccagg
     9001 ataccaaagc catctatctt tatgtttttc atttgtcatt ttacacgacc tccatcaaat
     9061 ttattttgtt gtcaattgtt cttttatttc ttcatctact cttatcactg tctttctatt
     9121 cgtcctgtgt actactagaa ggctaataag cgcgctaatc aacagcaaga tactgccatt
     9181 gacgaaaaca gcaagtgttt tactcccaag agaagcaaaa gagagaaaga gcaaaccata
     9241 tggtgcaatg aaagtaccta aattagcgcc aaccaataag attgaagtat tgaaactctc
     9301 cgcgtacttg ggcgcttgga tacttacttg gttgaataag taaggaatga ataacgaaaa
     9361 agaaattcct gttaggatcg caccaaaccc aatcacatac aggtttatca cagtggcaat
     9421 caatacaaag ccgattccca tcagtagtaa agaaagtgta agcatatagc gatgtgcttt
     9481 tgtctgaagc cagccaaaac aaactccggc agccatactt gccaattgca tgattgttaa
     9541 gatattcgct gcattagttt cgtttcctag agaataagtc gtcaggactg tggctaattt
     9601 aatcgataca gcattatata ccacgatgac cagcaagaga aaaataccat aactaatcat
     9661 tttaggattc gttttgatta cttgtcttgt tggggctgtg ccatgcgatt gaagttcagt
     9721 tgtttcaggt gtaggaacaa acaaaacaaa caagacaaga ataggtaagc tgatggcgta
     9781 gactaaaaag gtatattgcc aaccgaactg tactagctgt cctgcaacaa atgtcattaa
     9841 agctgcacca aatccttgaa atgcactttg aaaaccaatc atagaggctc gttcatctcc
     9901 gtgataaaag gcagcaatca tgctgactgc caaagaattg atcaatccaa agccaatacc
     9961 tagtccagct cttgaagcta agacaatact gtaactttgt gagaagaggg ggaggatacc
    10021 acaaatcgat gcaatcacta atccgatgat cacagtttgt ttttgtccga ttttcctagc
    10081 aataaaacta ctaatcaaaa caaaaatagt gaccataaaa gagggagcag tcgttaataa
    10141 ttcaacagca gataaagaac gcgtggcata agtagctgac atactgggaa ttgcagacgt
    10201 gactgcacca gcagaagtca aaataagaga gactgacagc agagaaaatt tcatcatctt
    10261 tttattcata agcatgttcc tttcattttt gtaagcgctt atcaaaaata tagtatgaag
    10321 tggttattct ctcaatagcg gaatcacagg aagttcatgt cccaaccata gaaagtcttt
    10381 atgaaaccaa aaatagcttt gcatataata gcagtacagc gtgaactaat aagcgaaaga
    10441 aaccgcttac aaaaaggagg gagtcaattg gaactagctg aattagacat tttttttgcg
    10501 taacgaagaa tcaatcgaaa ctctgcagcg tgaacaagga atgaatatca atgatttaaa
    10561 cttagcttac catcttcaga caccgccaaa aatccaccga ggggatctat ttcaacaggg
    10621 aaatattcat atcagtaaac accgtcgata tgcggaagtt cccgagcata cacatgattt
    10681 tgttgaaatc aattatatgt atagcggtac atgtttacaa attattaatg gagaaaggat
    10741 agagatggaa gaaagtgacg tggtgatcat tgataaagaa gctactcaaa gaatcgaata
    10801 cacagggcaa gaggatattt taatcaacat cttattaaaa gaagaaacct tatctacaga
    10861 gatattgaat catttggcaa gcacaaataa tctactttct tcttttttga taaatgcatc
    10921 aaaagaaaaa ggggaacata cacaattcat tcattttcgc ggcggaaaaa caagtcgttt
    10981 gacgcatttg attgaaaata tgctgttgat ttattttgat acatacagtc ataagatccg
    11041 ttcgttaaat ctctatacat cactattact gatcgaattg actcatttat tagaacaaca
    11101 aagcttttca tcagcagatg gatgcgcaga aaatgagatg cttagagtat tagattatat
    11161 cgataagcat tttacttctt gtacgttaaa agaaacagca gctcattttg gctacaatcc
    11221 ggtctatctt agtaataaat tgaaaatgta ttttgatgct tcctttcaag aactggttct
    11281 aaagaaaaaa ttcgactttg cgttggaact gattcaagaa acgagttttt cgttg
//
LOCUS       DACIRY010000088        11334 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.090, whole genome shotgun sequence.
ACCESSION   DACIRY010000088 DACIRY010000000
VERSION     DACIRY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.090"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..647)
                     /locus_tag="IYE22_001950"
     CDS             complement(<1..647)
                     /locus_tag="IYE22_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ9507155.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSR"
     gene            complement(864..1343)
                     /locus_tag="IYE22_001951"
     CDS             complement(864..1343)
                     /locus_tag="IYE22_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ9507156.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            1702..2484
                     /gene="rpsB"
                     /locus_tag="IYE22_001952"
     CDS             1702..2484
                     /gene="rpsB"
                     /locus_tag="IYE22_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ9507157.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            2583..3464
                     /locus_tag="IYE22_001953"
     CDS             2583..3464
                     /locus_tag="IYE22_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ9507158.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            3600..4322
                     /locus_tag="IYE22_001954"
     CDS             3600..4322
                     /locus_tag="IYE22_001954"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ9507159.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            4325..4882
                     /gene="frr"
                     /locus_tag="IYE22_001955"
     CDS             4325..4882
                     /gene="frr"
                     /locus_tag="IYE22_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ9507160.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            5077..5889
                     /locus_tag="IYE22_001956"
     CDS             5077..5889
                     /locus_tag="IYE22_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ9507161.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            5886..6686
                     /locus_tag="IYE22_001957"
     CDS             5886..6686
                     /locus_tag="IYE22_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ9507162.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            6847..8115
                     /gene="rseP"
                     /locus_tag="IYE22_001958"
     CDS             6847..8115
                     /gene="rseP"
                     /locus_tag="IYE22_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ9507163.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            8183..9892
                     /locus_tag="IYE22_001959"
     CDS             8183..9892
                     /locus_tag="IYE22_001959"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ9507164.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            10104..>11334
                     /locus_tag="IYE22_001960"
     CDS             10104..>11334
                     /locus_tag="IYE22_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ9507165.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVP"
BASE COUNT         3850 a         1962 c         2449 g         3073 t
ORIGIN      
        1 cgactcacat tttttcgctt ttctagttcc gctttattag caagtacaaa agaaaagcct
       61 ggaacacctt gaatacattt gtttgagctg gaaatcaaaa aatcaatccc tagttcggct
      121 acacgaatcg gtatcccacc aaagcttgac atcgcgtcca ctataaacaa aagatctttt
      181 tcttgtgtga cagcagcaat ctcttccaaa ttattcaaaa taccggaagt agtctcgcta
      241 tggaccattg ctagtgcgga gatagaggga ttctcttcaa tcagctgtgt aatcacatga
      301 gcttggagga tttgatgttc cgatacctca taaacggtat agttcagttt cagacattcg
      361 cacatgttga tcattcgttg tccatatgcg ccgtttgaac aaatcagtac agtatcttct
      421 ggcctgatca cagaacttaa taccgattct accccaaagg agccgctacc ttgcattaag
      481 accgttgtgt atattccttc cgatacttga gcaagatcca ataatttttt tcttatccat
      541 tgtgtaattt ctttgtattc ttgatcccaa gtacaatgat cgatcagcat agcatttttc
      601 actgtttcag aagtcgtcaa tggtccagga gttaataatt tatacatatg atttcctttc
      661 atttcttttt tgaaatacac aaagagcaag aagctgctct tgttaacatc caatataaat
      721 gatgattatt gtgattcaat caaggtgttg aaaaaattga ataaaaaatg atgtattttt
      781 gtaaagagtg taaaggttta tttaataagg aaaatattga aaataaaaaa acacgcattc
      841 catattagga atacgtgctc tgattaaaaa agtgatactt cagttggatg gtgaagaaat
      901 tcttctactc gttttgcgtc ctcctcattt tccgagatga ccatgatcgt atcgaatcca
      961 gcaattgtac tgacaataga gggaggtttg atctcatcta aaacagatgc aaataaatga
     1021 gcattgtctg gcaaggtatt gacgatcgtc aaaaagtgga ctcgttcgac tttcaataca
     1081 atgtcttcaa tcatttgaac cagccgatcc tcttcctcag tcctagtctc aggagttcct
     1141 ttttcgcgaa acaatacgaa gcgggaaacg ccattttcat caggtgcctt cacgattttt
     1201 agatcacgga tatctcgaga aatcgttgcc tgcgtggcgg taacgccata atgcccaagt
     1261 ttgtttaata gttcttcttg cgtacggatc gggaattctt caatgacttg tttaatcagc
     1321 aaatgtcttt gagctttgcg cattttcttt ctcctttacg aaagatttca catgtatcat
     1381 aacaaaaaaa agaaggattg cccaataata ttcgtctttt atacctaaaa ttattttatg
     1441 gaaaaaggat aataaagaaa caaagagaaa tctactgttt ttcttgaatt ctttttatct
     1501 ttcaggtaaa ctattgaatg ttgagcagaa aagtatattt gctcttgcga tacggatctt
     1561 ttgtctgtat aatagctgag ggtgctttat gcatcagcaa atccacattc ttattggatt
     1621 gtcggtaggt gcttgaaaaa gtttgccggc aaggtagaag ataagaagag gaaaaataaa
     1681 accaaatcgg aggaaacaaa catggcagta atttctatga aacaattact agaagccggc
     1741 gtacactttg gtcaccaaac tcgtcgctgg aatccaaaaa tgaagaaata tatcttcaca
     1801 gaaagaaacg gaatctacat cattgacttg caaaaaacag ttaaattagt agatgcagct
     1861 tatgattaca tgaagagcgt tgctgaagaa ggcggcgtag cattattcgt aggtacaaaa
     1921 aaacaagcac aagaagcaat caaagaagaa gcaactcgtg caggacaata cttcgttaac
     1981 catcgttggt taggcggaac attgacaaac tgggatacta tccaaaaacg tatcagccgt
     2041 ttgaaacaaa tcaatgcaat ggaagaagat ggaacttttg aagttctacc taaaaaagaa
     2101 gttgctggtt tgaacaaaga acgtgaacgt cttgaaaaat tcttaggcgg tatcgctgat
     2161 atgccaagaa tcccagatgt aatgtacatc gttgacccac gtaaagaacg tattgctgtt
     2221 caagaagcac acaaattaaa tatcccaatc gtagctatgg ttgatacaaa ctgcgatcca
     2281 gatgaaatcg acgtagtaat cccttcaaac gacgatgcaa ttcgtgccgt taaattgatt
     2341 acttcaaaaa tggctgatgc tttcatcgaa ggaaaccaag gtgaagatca agtagttgaa
     2401 gaagatttca cagaagaaaa cacagcaaca tcaatcgaag aaatcgtaga tgttgttgaa
     2461 ggcgacaatt cttcagcaga ataattttga atgatggagc tgtttcaaag gctgggcgac
     2521 aagcctcact ctcctttgaa gcagcttttt ttaaaaagaa aaacacacag gaggagttaa
     2581 acatggcaga cgttacagct aaaatggtaa aagaactacg cgaaatgact ggcgtaggaa
     2641 tgatggatgc gaaaaaagca ttagttgaag tagaaggcga catggaaaaa gcagttgatc
     2701 ttttacgtga aaaaggaatg gcaaaggctg cgaagaaaaa tgaccgcatt gcagctgaag
     2761 gattggcttc agttgctgtg aaaggtaaca ctgcagcaat cgttgaagta aactcagaaa
     2821 cagactttgt ttctaaaaac gaaatgttcc aagatttagt aaaagaaatt gcagaacttg
     2881 ttgctgaaaa caaaccagca gacatggaag ctgcaatgaa aatcaaaaca gataaaggta
     2941 caattgaatc tgacttgatc gaagcaactc aagttatcgg agaaaaaatc agcttccgtc
     3001 gctttgaagt tgtagaaaaa gacgacaatg ctgcatttgg tggatacttg catatgggcg
     3061 gacgtattgc cgtattgact gttctagatg gaacaactga cgaaacagtt gcaagagacg
     3121 tagctatgca cgtagcagct atcaatcctc gttatgtaaa cgaatcacaa attccagaag
     3181 ctgaattaga acatgaaaaa actgttctta cagaacaagc attgaacgaa ggcaaaccag
     3241 cgaatatcgt tgaaaaaatg gtagaaggac gcttgaagaa attcaaagca gaaatcgctt
     3301 tggtggacca accattcgtt aaagatcctg atatgactgt tgaaaaatac gttgcatcaa
     3361 aaggcgcaac tgttaaaaca ttcgtacgtt tcgaagttgg cgaaggtatc gaaaaacgcg
     3421 aagataactt tgtagaagaa gttatgagcc aagtgaaaaa ataattctgt tgaataacag
     3481 acgggaacgc acggtttgtg cgctcccttt ttttaagcaa aacaagaaaa accagtctta
     3541 gagtgtcgaa atcatctaag tatggtaaaa tggcaataga aaatattgga ggaatgaaca
     3601 tggtcacacc gaaatatcag cgtgtagtct taaaattgag tggagaagct ttagctgggg
     3661 atgcaggatt tggaatcaaa ccaccagtaa tcaaagaaat catccaagaa atcaaagaag
     3721 ttcacgagct gggcgttgaa atggcaatcg ttgtgggtgg cggaaatatc tggcgcggac
     3781 aaatcggagc acaaatgggc atggaacgtg ctcaagctga ttacatgggt atgttagcta
     3841 cagttatgaa tgccttagct ttacaggata cgttggaaaa cgtgggtgta ccgacacgcg
     3901 tgcaaacttc gattgaaatg cgtcaaatcg cagagccata tattcgccgt aaagcagaac
     3961 gtcatttaga aaaaggacgg atcgttatct ttgcaggcgg taccggcaac ccgtacttct
     4021 cgacagacac aacagcagca ttacgagcgg cagaaatcgg cgcagatgtt atcctgatgg
     4081 ctaaaaacaa tgtagacggt gtttattctg ctgatccgaa agtagatgca aatgcagtaa
     4141 aatttgaaga actgactcat ctggaagtta ttgccaaagg attacaagtc atggattcaa
     4201 ctgcaagttc actaagcatg gataatgata ttccactgct tgtcttcaat ttaaatgaac
     4261 acggaaatat tcgccgtgca atcttaggcg aaaatatcgg aacaactgta agggggaaat
     4321 aaaaatggca gatgcaatta tgacagaagc aaaagaaaaa atgaaaaaag cagcacaaaa
     4381 tcttcaacgt gaacttgggc aaatccgcgc aggccgtgcg aacgctagcc ttttggaccg
     4441 tattactgtg aactactacg gtgcgccaac accattgaac caaatggcat caatccaaat
     4501 tccagaagca cgtgttttaa tgatcacacc ttttgacaag agcattcttc aagatgttga
     4561 aaaagctatt atggcaagtg atatcggaat cagtccaacc aatgatggaa atgtcattcg
     4621 cttagttatc ccgcaactga cagaagaacg cagaaaagaa ttagctaaag atgtgaaaaa
     4681 agaagcagaa aatgcaaaaa tcgcgattcg taacatccgt cgtgatgcga tcgatgaata
     4741 taaaaaacaa caaaaaaatg gtgatattac cgaagatgat ttgcgcggat tagaaaaaga
     4801 cgtacaaaca ttgacagata acagcattaa agaagtagat aagatcgctg ctgataaaga
     4861 aaaagaatta ttggaagttt aataaaatca taaaaacaga ggggcggatc attccgcttc
     4921 ctctgttttt atttatattt cttttattta aaaattaaaa aaagaaaaaa ttattcagtt
     4981 tttaaataaa aaaacaatga tttcatagga aaaacagtta gatattgcta taatacttaa
     5041 gatagtggga atcttttaag tataggagga aaaagaatgt tacgtttttt tccgcaaaaa
     5101 aacaaatata ttcaagaaaa aagcgaattt caatttaata ccgatggacc aattcctcag
     5161 cacattgcca tcatcatgga cggcaatggt cgttgggcgc aaaaccgtcg tttgccacga
     5221 gttgctggcc ataaagaagg catggaaaca gtaaagaaag tcacaaaaaa agcatcaagg
     5281 cttggagtga aagtgttgac gctttatgcc ttttcgactg aaaactggaa gcgaccaaaa
     5341 gatgaagtaa gttttctcat gcaactacct gtcgacttct ttgatacgtt cgtaccagaa
     5401 ttgattaaag aaaatgtcaa agttcatgtc atgggttatg aaaacgtatt gccagaacat
     5461 acgcaagatg ctgtaagacg tgcaattgag caaacaaaaa acaatactgg aatggtattg
     5521 aattttgctt tgaattatgg aagtagagca gagatcgtga ctgctgtcca agaaattgcc
     5581 gaagaagtag caaaaggaga aatacacgca gaagaaatcg atgatgaatt gattgctaag
     5641 catttaatga caggtttctt gcctaaagag ttacaagatc ctgagctgat gatccgtaca
     5701 agtggagaag agcgaattag taattttctg ttatggcaga tcgcatatag tgagctgtat
     5761 tttacaaaag cgctttggcc tgattttgat ggtgcgcatc ttgaagaagc aatcgcctct
     5821 tatcaaaatc gagaccgtcg attcggcggt gtaaaaaaaa cagaagatga gaaaggggat
     5881 cagtcatgaa acaacgtgtg atcacagcag ctgtggcgct agtcttgttt attccgatta
     5941 tatggatcgg aggcatagcg gttgagctta cagcagcaat cttagcggta gtaggagttt
     6001 acgagctgtt ccggatgaaa ggattgacac tcttaagttt tgaaggagtt ttatccgccg
     6061 ttggtgcagt gttacttgtt ttgccaaaag aaagatggtt tttctttctt ccagagaatg
     6121 taagtacatt tatgttattt tatcttatag ttatgattct tttaggaaca tcagttattt
     6181 ctaaaaatac gtatacgatt gatgaagcag gttttcctgt gcttgtcagc ttgtatgtgg
     6241 gaataggttt ccagaatttt gtcaatgcac gaacagaaag cttggctgtt ttgatatttg
     6301 gtctatttat tgtttgggca actgatatcg gagcatatat ggttggtcga cgttatggtc
     6361 agcataagtt aagtcctgat atttcgccca acaaaacgat tgaaggtgcg ctcggtggta
     6421 tcggtagtgc tgttgtagtc gcatttcttt atttattgct atacccagca aaagaactat
     6481 tcaatcatgg tatgtttgtc atgcttctga tgacgattat tttttcagca gtcggacaat
     6541 ttggtgattt ggtcgaatct gcaatcaaac gtcattatga tgtgaaagac tcgggaaata
     6601 tcttaccagg ccatggtgga atcttagacc gtttcgacag tttgctgttt gtatttccta
     6661 tcatgcattt atttggcatt atttagtaag tatacctgcg tttctttgtg aatttcataa
     6721 aagacgcagg tttttatttt tcattatatt gatactattg gtgtctttgg tatactatgg
     6781 taaaattata gtgttttatc gttgttttat aaaaaacaca aaaaacaagt aaaggagaaa
     6841 aaagctatga aaacgattct gacatttatc atcgtttttg gtatattagt gattgttcat
     6901 gagtttggtc atttcttctt tgcgaaacga tcaggaatcc tcgtccgcga atttgctatc
     6961 ggtatgggtc cgaaaattta tgggcatcaa gcaaaagacg ggacaacata tacgcttcgc
     7021 ctattaccta ttggcggtta tgttcgaatg gcaggcaatg gggatgatga aacagaaatg
     7081 gcaccaggaa tgcctctttc tcttttacta aattctgacg gcatcgtaga gaagataaat
     7141 ttaagtaaga aaatccaatt gacaaatgct atcccgatgg aattgagccg ttatgattta
     7201 gaagatgagc tgactatcac tggttatgtg aatggagacg aaacagaagt agtaacctac
     7261 cctgttgatc acgatgcgac gattattgaa aacgacggaa cagaaatcag aattgcgcca
     7321 aaagacgtac agttccaatc ggctaaattg tggcagcgta tgctgaccaa ctttgctgga
     7381 cccatgaata actttatttt ggcaatcgtg ttattcatca ttctggcatt tatgcaaggt
     7441 ggtgttcaag tgacgaacac taatcgcgta ggagagatca tgcctaatgg agctgcggca
     7501 gaagcaggct taaaagaaaa tgatgaggta gtcagtgtgg acggaaaaga aatccattca
     7561 tggaatgatc tgacaacagt cattacgaaa aatccaggta aaacattaga ctttaagatc
     7621 gaacgagaag gtcaggtgca atctgtcgac gtgacaccga aatctgtaga gtcaaatggc
     7681 gaaaaagttg gccaacttgg tataaaagcc ccaatgaata caggttttat ggataagatc
     7741 attggaggga cgcgtcaagc tttcagcggt tcattggaaa ttttcaaagc cttaggatca
     7801 ctatttacag gtttcagttt agataaatta ggcggacctg tcatgatgta ccaattgtct
     7861 tcagaagcag caaatcaagg tgtaacaact gttattagtt tgatggcgct tctttcaatg
     7921 aatctcggaa tcgtcaatct gcttccgatt cctgccttag atggcgggaa attagtcttg
     7981 aatatttttg aagggattcg cgggaaacca ttaagtcaag aaaaagaagg aatattgaca
     8041 ttagctggct ttggattttt aatgttgttg atggtgctag ttacatggaa cgatattcaa
     8101 cgattctttt tctagaaaaa gttgacaaat agggtataat aacaaacgat aactattttg
     8161 aggaaataaa ggagtatata gaatgagaca atcaaaaatg ttgattccaa cattacgcga
     8221 agttccaaat gatgcagaag tattaagcca tcaaatcttg ctaagagctg gatatatccg
     8281 ccaagtatca gcgggtattt attcatatct gccattagca aatcgtgtat tggaaaaact
     8341 gaaacgcatt atgcgggaag aatttgaaaa aatcggagcg gtagaaatgc tgatgcctgc
     8401 catcttgcca gcagaacttt gggaagaatc cgggcgttac gaaacatatg gaccaaactt
     8461 gtatcgtttc aaagaccgca atgatcgaaa aatgatcctt ggaccaactc acgaagaaac
     8521 ttttactgat ttgatgcgta atgaaatcaa ttcatataag aaattacctt taaatcttta
     8581 tcaaatccaa cctaaatatc gtgacgaaaa acgacctcgc ttcggtttgc ttcgcggaag
     8641 agagtttatc atgaaagatg catattcttt ccatgcaacg gaagaaagtt tggacgagac
     8701 atataaagat tacgaaaaag cctataccga agtattcaag cgttgtggct tagaatttcg
     8761 ctcgatcatc ggagacggcg gcgcaatggg cgggaaagac tcaaaggaat tcatggcgat
     8821 ctcagaaatc ggggaggata cgatctgtta ttcgactgaa agtgattatg ctgcaaactt
     8881 ggagatggct acaagctact atgtatcaaa aaaatcgcat gaaacacagc ttgaattgga
     8941 gaaagtagct acacctaacg taaaatcaat tgacgaagta gcagatttct tttctgttga
     9001 acctcaaaaa atcatcaagt ctgtattgtt cattgcggat gaacaacctg tccttgtttt
     9061 ggtacgtgga gaccatgaag tcaatgacgt aaaactgaaa aactttttat ctgctgactt
     9121 tttagaagaa gcaacagaag aagaagcaca aaaatatcta ggtgctgagt ttggttctgt
     9181 cggaccagtc ggtgtttcag atgacgtaaa agtatatgca gaccgttatg tccaagattt
     9241 agcgaatgcc attactggag cgaacgaaac aggattccac tataaaaatg tgaatccaaa
     9301 acgcgatttc actgtgcttt cttatgaaga tcttcgtttt gtacaagaag gagatccttc
     9361 accagacaac aacggtgttc ttgcctttac tcgaggaatc gaaatcggac atatattcaa
     9421 aatcggcact cgttacagtg aatctatggg agcaactgta ctagacgaaa atggacgaga
     9481 aaaatttgtg attatgggct gttatggtat cggtgtaagt cgtttattat cagcaatcgt
     9541 agaacaacat tcagatgaac aaggaatcaa ttggccaaaa ggaattgcgc catttgatct
     9601 gcatatcgtc caaatgaatc taaaagacga ataccaaaca agcctgacga atgaactaga
     9661 agaagcgatg acaaaagctg gctatgaagt actggttgat gacagaaatg aacgcgcagg
     9721 agtgaaattc gcggattctg atttgattgg ctgtccaatc agaatcactg tcggtaaaaa
     9781 agcagtggat ggaatcgtag aagtgaaaat caagaaaact ggcgaaatgg ttgaagtacg
     9841 taaagaagaa ttggcgaata ctttaccaat cctcttgaac caaggcgaat aataagcgtt
     9901 aaaacacaga aaataaaaat agctgaatca gctgtttttg acaatctgat gattcgcttt
     9961 taaaattgga actgcgattt gaatcgcagt tccaattttt taatgtaaaa ctcagaaaaa
    10021 acgagtatac taaaaggtac tgaaaaagaa gaggataaga tgcgatcaat tgcattgttt
    10081 ctcatcagaa agggcgtata tctttgtcag aaaaacgaga tttatttgaa aaactgttgg
    10141 aacaaatcca actagaggaa gcagaaaaaa atcatccact gcttactgct ggtgaaatgg
    10201 atagtgtagt ggtccatcgt aaatcgcgtt tatgggaatt tacgctacat ttttccaaaa
    10261 ttttgcctat tatgctatat cggtcattga ctcagcatct gactatcgct tttaaagata
    10321 ttgcacaagt caaactgaat atcttagcag atgaccagac attcgatgaa cagctcttgc
    10381 aagattactg ggcccaagct ttggaaaatc agcaatgcga tacaccacta gcacagcaag
    10441 tattgaaaac gcaggtacct gtgataaaag atcgtaaagt catcttgcca atcgacagta
    10501 caggagctat ttcttatttg aagcaacaat acttgccatt agtagaagag ttgttcgtca
    10561 gttacggatt cccaaaattt cgtatcgaac cagaagtgga tgagcagcaa gcggaacgtg
    10621 tattgaaact ttttgaagaa cgcaaacaag aacaggcaga agcctttatg aagcaagcag
    10681 ctgaaagtct agttgtccac gaacaaaaga aaaaggaaag aaaagagcaa tcgccagctc
    10741 ttgaaggacc catccagcta ggacgaaata ttccagccga tgaaccaacg acaccaatga
    10801 tcagtatcgt tgaagaagaa cgaagagtca cacttgaggg gtacgtattc gacaaagaag
    10861 tcagagaact acgttcaaaa cgcaagatct tgactttgaa aatcacagat tatacttcat
    10921 catttatcgt caaaaaattc tcgaacggcg aaaaagatga acaagtattt gatgcgatca
    10981 gtgtcggtag ctggttaaag gtacgaggaa gtattcaaga agatacgttt gtccgtgatt
    11041 tggtcatgaa tgcgcaagat atcatagaag tcaaacatac acctcgcaaa gattatgctc
    11101 ctgaagggga aaaacgagta gagcttcatg ttcatagcaa tatgagtacg atggatgcaa
    11161 caaacagcat ctctgatctt gtggcacaag caggaaaatg gggacaccga gctatcgcca
    11221 ttacagacca tggtggtgcg caagcctttc cggaagcaca tagtgctgga aaaaaagcag
    11281 gtgtgaaaat cttatacggc gtggaagcga atgtcgtaga tgatggtgtc ccaa
//
LOCUS       DACIRY010000089        11247 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.091, whole genome shotgun sequence.
ACCESSION   DACIRY010000089 DACIRY010000000
VERSION     DACIRY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.091"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(3..2012)
                     /gene="metG"
                     /locus_tag="IYE22_001961"
     CDS             complement(3..2012)
                     /gene="metG"
                     /locus_tag="IYE22_001961"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ9507166.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2280..2558
                     /locus_tag="IYE22_001962"
     CDS             2280..2558
                     /locus_tag="IYE22_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507167.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2655..3476
                     /locus_tag="IYE22_001963"
     CDS             2655..3476
                     /locus_tag="IYE22_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ9507168.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3761..4771)
                     /locus_tag="IYE22_001964"
     CDS             complement(3761..4771)
                     /locus_tag="IYE22_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9507169.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4783..5736)
                     /locus_tag="IYE22_001965"
     CDS             complement(4783..5736)
                     /locus_tag="IYE22_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9507170.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5726..6796)
                     /locus_tag="IYE22_001966"
     CDS             complement(5726..6796)
                     /locus_tag="IYE22_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507171.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6802..7845)
                     /locus_tag="IYE22_001967"
     CDS             complement(6802..7845)
                     /locus_tag="IYE22_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9507172.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7846..8787)
                     /locus_tag="IYE22_001968"
     CDS             complement(7846..8787)
                     /locus_tag="IYE22_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9507173.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8871..9218
                     /locus_tag="IYE22_001969"
     CDS             8871..9218
                     /locus_tag="IYE22_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ9507174.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9288..10949)
                     /locus_tag="IYE22_001970"
     CDS             complement(9288..10949)
                     /locus_tag="IYE22_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9507175.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3173 a         2287 c         2046 g         3741 t
ORIGIN      
        1 ttctatgcga tttctgagcc atttggcatg gattttggtg cttcgattac ttgtaaagag
       61 ccatctggtg cttctgcaga aaggatcatt ccttgactga tttgtccacg catctttcgt
      121 ggtttcagat ttgctacaat gacgactttt ttcccgatca attcgcttgg atcaggataa
      181 aattcagcga ttcctgacaa gatttgacgg tcttggctgt ctccagcatc tagtcggaat
      241 tgcagcaact tatctgcccc ttctactttt tgacagttga tgacttctgc tacttttagt
      301 tctacttttt caaatacatc aaatttgatt tgtttttctt tcgttgaaac aagttctgtt
      361 tcttctggat cccatttgac tgtatcttcg tttgtttgag taccttcaga cattttttct
      421 tgaataaaag caacttcttc ttccatatct aaacgtggga agattggtgt accttttgca
      481 actacttttg ttcctgaagg aaattcgcca aagtgtaatc cttcaagatt cattgtttca
      541 gagtccaatc ctagttgatt gaagatcttc tctggcgttt ctgtcatgat tggctgaagt
      601 aatatcgctg cgatacgcaa agattcagct aaatggatca tgacactatt caattcagct
      661 ttcttttctt cgtcttttgc taatacccat ggttctgttt catcaatgta tttgtttgca
      721 cgagaaatca gcacccagat ttcagccaat gctgtattga attccatttt ttccattgca
      781 tcatggtaac gtccgacaac gttagcagcc gtcgtagaca gttcactatc aaatggagtg
      841 acaagtgagg catagtcagg aacgagtcca tcacaatatt tgttgatcat agcaatcgtt
      901 cgattcaata agttccctag atcatttgcc agatcataat tgacacggga aacaaaatct
      961 tctggcgtaa agacaccatc tgacccaaac ggaacagcac gaagcaaata ataacggagt
     1021 gcatctaaac cataacgttc cacaagcatt tcaggataaa cgacatttcc ttttgattta
     1081 gacatttttc cgtctttcat caatagccag ccatgcccaa atactttttt aggtagaggc
     1141 aaatcaaggg ccatcagcat gattggccaa tagatcgtat gaaagcgaac gatttcttta
     1201 ccgaccatct gaacatctgc aggccagtat ttttcgaata gactttcatc ttcggagcca
     1261 tatcctagtg cagtaatata attggataat gcatcgatcc atacatacac cacatgtttc
     1321 ggatcactct tgactggtac tccccaagta aaagttgttc gtgaaactgc aagatcctct
     1381 aatcctggtt tgataaagtt gttgatcatt tcgtttttac gagattctgg ctggatgaat
     1441 tcgggatgtt cttcatagta ttccaataga cggtctgcgt atttgctcat gcggaagaag
     1501 tatgattctt cttttaccaa ttctacttcg tgtccacttg gcgctttacc accgatcacg
     1561 ttaccttcgt catctttgta cacttctgca agttgtgttt ctgtaaagaa ttcttcgtca
     1621 gaaacagagt accagccttc atattcacct aagtaaatat ctccctgttc taataaacga
     1681 tcaaatattt tttgaacagc tgctttatgg taatcatctg tcgtgcgaat gaatttatca
     1741 taactgatat caagcgtttt ccataatttc ttgacatcag ccgccatttt atctacatat
     1801 tcttgcggtt ttacacctag ttcttctgct tttgtttcga ttttttgacc atgttcatcc
     1861 actccagtga gataaaacac atcaaagccc atcaaccgtt tataacgagc aattgcgtca
     1921 catgcaatcg tagtatacga gttgccgata tgaagtttgc cgcttggata atagattggc
     1981 gtagtgatat aaaaagtttc cttttctgcc acggggctct tcctccttta tcgcatgatg
     2041 cgaattcgtt tccttgctag tataccataa aaaaagcatg ctgttagctt tttttcaaaa
     2101 tgaaaaagac tggataaacg acttctatct acgttcaatc tatcctttaa aaggatgaat
     2161 aggttgaata aatgcgggtt tttgttttgt ttcatatcta ctaaaaaacg gctttttatt
     2221 tctcccctca cacgtggtat aataaagact atctatctta gtaagggact ggaacaatca
     2281 tgcagaagaa acatcttttt tttacactta gtattgcttt tctttcatta gcacatttaa
     2341 tattcagcta tttttatatt cgtatgtatg gttattttaa cctccatggg catttgaaca
     2401 gttttatgac agcagcttgg attttgcgtt ttatcattga cgtatatatc gttatttgtg
     2461 gcttctttgc cattcgtgaa gagcggtata aagtattgcc gttttatctg cttttctttt
     2521 tattcaatct catacttcca tttatttttc atatttaaat gataacctag atttttcaag
     2581 taaatccttg gttttttttc agagtctgct acaataaggt aaaaagtact tttactgtca
     2641 atggaggtgg tcagatgaac cgttttgctt ctaaagaaga agaaaaatta tattatgaac
     2701 ataaagcaag tgaagaagaa tttcttaact ggtatcaaaa acaggaacgt ccagaatatg
     2761 acaagccctc tttgactgtc gatatcgtac tgatgtgcta taacaaagaa gaagatcagc
     2821 tgaaagtttt gttgatccaa agaaaagggc atccttatcg aaattcatgg gcattgcctg
     2881 gtggctttgt ccaaaaggat gagtcaactg gagaaagcgt cttaagagaa acaaaggaag
     2941 aaactggtgt ggttatttca aaagaaaata ttgagcaact gcatacattc agtacgccta
     3001 atcgagatcc gcgaggctgg gtcgtcacag tcagctactt agcctttatt ggagaagaac
     3061 cgctaatcgc cggagatgat gccaaggaag tacgctggtt cacactagaa cgtcatagta
     3121 acaagatcca tttatctagc ggagaagtgg ctatctcgct tgatttagtc actggagagt
     3181 cttctggcaa agatactttg gcttttgacc atagtcaaat catcttgaaa gcattcaacc
     3241 gtgtagtcaa taaaatggaa cacgagccgc aagttctaca agttttaggt aaagatttca
     3301 cgattacgga agctcgaaaa gtatttgcta aattcttagg gatcgattat aaaacgatcg
     3361 atcattccaa ttttaagaaa gctatgctcc aatatttcga tgagatcggt gaacgtcctg
     3421 taggaatcgg ccgtccttca aaaatctatc agctcaagcc ggggcaccat gaataaggtt
     3481 gtgagcaaaa gattcagacc taatcattca agaaaaaacg caaaaacaac tctttgtgag
     3541 ttacgttttt tgtttcagaa ttttgatgta ttcgtaaact ttctgaataa aaaattaaca
     3601 atagcagaaa aagctcgtac gaactttttc tgctattgtt ttattggttt gtcataatta
     3661 gtgagctttt cttcaaaaga aaaattccta gaaacaaaag tcattaagac tttttattaa
     3721 caaggctaac cctaacgctc ctatcagttt cattcatttt ttatcccttg ataaccgaat
     3781 gtctgcccat tttttctact acttggctct ttcttacgga attgctttct tctgctccgt
     3841 cggtattgat caaaagaaat tttttattat accattggga cttataacgc aattttgtta
     3901 ttaatttatg ccagtcgtga atagaaaaat cagcatgtgc ctcttctttt ggaaaagatt
     3961 ctacaaataa gtagtcatat tgtttgacca atttcccaga taattgttca agataatctt
     4021 ctcgctggtt cttttgtttc agataaagat ccattacttt tctcttttgt ttctgataat
     4081 tgcttgcctg atctaacctg atctttctat ggtgagcgga gcggactttc acattcaatt
     4141 tccgctgtgc atgttgcagc ttttcttctg tctggtgaaa ttttggtaaa gttacttcta
     4201 gaggtcgaga tgtttggatc aatgtcttcg gatgataggc gatcccgatc catttagcta
     4261 aagattcttt ttttactggc gaaacatcac aaagtagtgc gacataaaat tcttcattat
     4321 atcgagcaga aatcgtagcg gagcgaatcg atccttcaac tggccgatgc tgatttatct
     4381 tgattagttc tttttgtttt ggtaacttta agtagccatt ttccagatag atcgtacggg
     4441 tttggttgtt ggtcgtataa gattgccaca tacttttttt cgttttaagc tttgggaagc
     4501 tggcacgtcc cttgaaataa ttgcgaaaag cccgatccaa attaagctgt gcatttgcta
     4561 aagcaagact gtctacttct tttaagaatg ggtattcttt ttttaatgta gcgggtgtga
     4621 gcgaaaaatt ggctactcct gtttgttgcc aacttttctg ccgctttttc agcaaatggt
     4681 tataggtaaa tcgagcacat ccaaaagtac gaatcaattg ctgtttttgt tcttctgtcg
     4741 gatacatacg gaatttaaaa gcttttagca tctatgtccc tcctagttag caaccagaag
     4801 agattccttt tgtcgtttta gtttttttgc gtaatatggg acttcctgct cagttgcata
     4861 gacaaaatgt tctggtgcga tttcacgaag ttgacgtact tgtccgtcat ctggttcagg
     4921 ttgatatttg attcttctgc gttttctctc atggtctgga tctggtagag gaatagcaga
     4981 taacaaactt tctgtataag gatgaacacc gtgattatag atttcatcac ttgttcccat
     5041 ctctaataac aagccgttat gcattacacc gattcggtca ctaatatgtt taaccatcga
     5101 caaatcatgt gcaataaata gataggtcaa ttgatgttct ttttggagat cttgcaacaa
     5161 attgaccact tgggcttgga tcgacacatc tagtgcagaa attggttcat cacaaatgat
     5221 aaaacgcggt tttactgcca atgctcgtgc aataccaata cgctggcgtt gcccacctga
     5281 aaattcatga ggataacgag taccgtggct tggattcaat ccaacagttc tcaatagatc
     5341 atcgactttt tttgcacgct cttcagatga tttagctagt ccattaacat caattccttc
     5401 ggcaatgata tctctgactt tcatacgagg attcaacgaa gcgtatggat cttgaaagat
     5461 catctgaaca tcatgccgga atttcgtcaa acctttttta tctttgatct tcgttatatc
     5521 ttgaccgtcg aataatatct caccatcagt aggttcattc aagcgaatga tcgtacggcc
     5581 agtagtggat tttccgcttc cagattctcc aactaaaccg aatgtttctc cttcataaat
     5641 gtgaaagctg atgtcattta cagcatgtac ttcattcttt tttcctacat tgaaatactg
     5701 tttcaatcct ttaacttcaa gtagtttatt tggcatcagc aattcctcct tgaccgtaag
     5761 tatgtttttg tttctcttta aagatagccc agcgacgttg gatttcttct ggcggtgtca
     5821 ctttgggtgc ctgcggagca agtagccaag ttgctgcctt gtgtgttttt gatagttcaa
     5881 aaaatggtgg ttcttgttct gcatcgattt tcatcgcgaa ttcatttctt ggatagaatg
     5941 cgtctccttt tggcgggttc aataaatccg gtggagaacc aggaatagca tacaatcgat
     6001 cgttcgcact atctaatgtc ggcattgaac caagcagtcc ccaagtatag ggatgttgcg
     6061 ggttgtagaa aatttcttcc gatgttccaa cttcaacgat tcttcctcca tacatgacag
     6121 cgacacgatc agcgacattc gctactacac caaggtcatg agtgataaag ataattgatg
     6181 tatcgatttt tgtctgaatt tctttcagca gctccaaaat ctgtgcttga atagtcacat
     6241 caagcgctgt tgtaggttca tcagcaatca ggatttccgg ataacatacc agtgcaatcg
     6301 cgatcacgat ccgttgacgc tgacctcctg agaactgatg cggataactg ttgatacgtt
     6361 tctcagcttc gggtattcca actaatttaa gcaattctaa tgcagctttt tgtccctctt
     6421 tttttgaaac tttattatgc tttctaaggg actcagcgac ttgcttccca atgggcatcg
     6481 tcggatctaa agaggtcatt ggatcttgga aaatcatcgc tatttcttta ccacggattt
     6541 tttgcatctc tctttctgat tttccaacga tatcgttacc tttaaagagg atttgaccag
     6601 catctatatt ggcgttgctt gacagcaagc gcatgatgct tctggtcgtc actgattttc
     6661 cacttccaga ttccccaacg atcgccaatg tttctccttt attcaaatga aaattgacac
     6721 cgcgaatcgc tcgtaccttt ccagcatacg tatcaaaaga gatttctaag tctttcactt
     6781 ctaaaatttt cgtcatcgtc ttcactcttt cattttagga tcaaaggcat cacgcaatcc
     6841 atcagcaagt aagttaaagc caatcatgat aaccgataaa gttactgccg gaatccataa
     6901 tagataaggc agatacagga atgtcttgta cccttcgctc aacatcgttc ctaatgaagc
     6961 tgacggaggc gtaagaccta aaccgataaa gcttaaaaag gcttcgaaga aaatagcaga
     7021 tggaatacta aacatcattt gaataataat gacactagag atattcggca aaatatgttt
     7081 gaatgcaatt ttcattcttg attcacctaa tgtcaatcca gctaaaacat attcttgatc
     7141 ttttaatttc attgtttgtg cacgaacgat acgtgccatt ggtatccagt ttgtgatggc
     7201 catagcagcc acgattgaaa aaattcctgg ttcaaaaacg accaacatca aaatcatgac
     7261 aacgaggttt ggaattcctg ataagatttc caaaaaacgt tgcatcacgt tatcgacacg
     7321 tccgcctaac aaaccagaaa ccaacccgta agctacacca atcgttacat ctagtaatgc
     7381 agcaataaat gcgatcagca acgagattct cattcccata aataagcggc ttaatacatc
     7441 acgtcccaaa ccgtctgttc ctaaaaagaa atttacgttt gaagggacgt ttgcttgagc
     7501 atatttatca acaagttcac caccaaccat tgcttttcca ttcaatccat cgatattgat
     7561 tcccggaata cgaggaggca aattgatata agccacgttt tgggcagttg gatcatgagg
     7621 agaaacaaag attgtgatca cagagatcaa aatgatgacc aatagtaaaa ccattgaaat
     7681 aacggcagct ttgtttttct ttaaacggcg ccatgaatct tggataaaat tcaaagaagg
     7741 tgcagtaatc tgttcacgtt cttcttcggt atttttttgt aatggaagaa attcatccgc
     7801 agggatatct gcaactgttt cataacgctt tggtatcatt tccatttatc ctcggctccc
     7861 ttctgaaaca cgaattcttg gatcaactag accgtacaat agatccacga tcaaaataac
     7921 aaaaatcagc ataaatgagt aaaggatcgt tacagccatg atcgtaggat aatcatttgt
     7981 tgtgattgac ttaacgaatt gttccccgat accaggaata gcaaagatat tttctaccac
     8041 cagtgatccg gtcatcaatg ctacagctaa cggtcctaat aaagtcatca atggaatcaa
     8101 actatttcga agaccatgtt taaaggcgat ttcccaacgg ctcaatcctt ttgcttttgc
     8161 taattccaca taatcactat gcagtacttc caccatttcc gtacggataa accgagcaga
     8221 gtcagcaagc ggcgacatgg ccaacgcaat tgttggtaag attgtgtaca caaagccatc
     8281 ccattttgcg attggcaaaa cttgtaattt gattgcaaag atatattgta ataaaacagc
     8341 aaatacaaag tttggaatgg aacgacctaa tatcgctacg agtgtagaag atgtatctgc
     8401 ccatgtattt tgcttcatgg cagagatcgt tcctaaaatc gtccctagga ctgttccaaa
     8461 gataattgcc tgtaacccta actgcaagga tggtccgatt cttccagcta acaaatgtgc
     8521 gactggctgg tttttaaatt ggaaggaaat tccaaaatct ccttgaagca aattagaaag
     8581 ataaatccca tattgtacga taactggttt atccaaaccg acttgtttat ttagcatctc
     8641 gattgtttca ggactcaaac gttcctgatt cgtatatggc gtgcccggca gcaattgcat
     8701 caagaaaaag gtgattgttg caattaacca aagcgtgatg atcatgaaaa agacacgttt
     8761 taatacatat ttgatataac tattcactaa tgtttccccc tagcttttga cttctgacag
     8821 atgtttcgct acattattag ataatgaacc aaaaataaag gagcaatcaa atgaaaaaaa
     8881 aatctatccc ctacgcagta gcttttctac tgattctggt tattttaata aaaaatctca
     8941 tcaaccattc ctttacactc attcaattat ctaatgatct attcttatgg tcattgccgt
     9001 ttctgattat cggcggtttt ttatgggtct tttcttcggg tttcttcgat cattttcagc
     9061 gatcggtcca tcttgcacga acaaggaacc gaaagaaaaa accagaattt tcctcgctct
     9121 cgtctgccag ctacggtatg tacagctttt ggctgatcat tgccggaata ctaattgctc
     9181 tttctgctat tttcatgctt ttttctttgt taggataagt gaatatgaga acgaggttgc
     9241 gacatttgcc gcagcctcgt tttttctcat gctcacggta ttaacgatta ttcttcaacg
     9301 tatgtccatt tgtaatccca tgaagcgcct gccgcatggt gaacgatacc tttgattttt
     9361 tcattcacta ggtgaccttc tgctttttgg tacaccggaa cgacacccat atcatcagag
     9421 atgatcttga ttgctttttg gaaatcatcc caacgggctt gttcatcgcc agcgtctgtt
     9481 gatgaagctg ctttaactgc ttcgtcatat tctgcattgc tccagcgacc acggttgtat
     9541 gaattacctg tgacaaacaa atcagtaaag ctgcttgggt ctgcatagtc agcagcccat
     9601 ccgcctagta ctgcgtcaaa atcacccgaa gttgagcggt caagacgtac agagaatgga
     9661 acaggtgttg gtttgactgt tacgccatcc aagttttctt ggattgagtt ttgaacatat
     9721 tcgattactt ttttcgatga atcatcatca gaagccatca aatcaaattc taaagaatca
     9781 acgcccaatt cttttttcgc tttttcccaa tattcttttg ctttatcttt gtcataagaa
     9841 actaattttc cagcttcttt tgcaaagtct tcgttcgttg ttggactttt taccatatct
     9901 gatgggatca atcctgtaga gacaactgat ccgtcaccta gtacttgttt tactaaagct
     9961 tcacgatcga tagaataaga aatagctttt cttaggttca cattgttgaa tggtgaattc
    10021 ttttcacgtt gattgaattc gatatagctt gtccgcgctt ctttaacaga agtataagct
    10081 ggatcgtttg cgttttgctg tgctaactca ccagacaaga tcacgtcatc tgcttggcca
    10141 tctttgaata agttcaatgc agtagaagat tcttttacta cactgacttt gatttcagaa
    10201 agttttactg tatctttgtc ccagtaatga tcatttttct tataagtcca ttctgtatct
    10261 gttcctggtc catcaaaatc ttctaatgtg aatggtccat tataaacagc tttatcactt
    10321 gcagatgcat aggcatcccc attttcttcc actacggatt gtttttgtgg gaaaaatgat
    10381 gggaaagcaa gcagatattg gaaatatggt gtttgtttcg tcaatttgat ttctaattca
    10441 tagtcaccaa ctgctttgat ccctagttct gatttatctt tctttccagc tgtaatgtct
    10501 tctgcatttt caactggcgc atacaggtaa gcatattcag aagctgtagc tgggtctact
    10561 gttcgttgcc agccataaac gtaatccgct gctgtaacag gatcaccatt tgaccatttt
    10621 gcgtcttctc gtaatttgat tttgtaagtt aatccgtctt cactgacttc tgccaactcg
    10681 ctagctcctg ccggctgtgg tttgctgtct tcatcaagac ggtaaattcc ttcataaacg
    10741 ttattcaatg ctgtaaaact gattgtatct gttgcaactg ataagtcagc tgtaggcatt
    10801 tcttgaggca cgactaagtt aaatacgcct cctccatctg ctgaaccgct ttttgctgaa
    10861 ctactgcttg tggctgaact gccgccataa catccagcaa gtacaaggcc gcataccgca
    10921 accaaaccaa atgtaaattt ctttttcatt ttacgtaccc ctttattgga attttcagaa
    10981 taattaatat tttctgaagt tgtttaagag tttaatgaat aattattaaa aaatcaacac
    11041 ttttttttgt ctttttcata aaataatgat tttttcgtga ggtttcaagg aatttttcag
    11101 aaaaaatagc tttgtacaag gttttccatg gtcttccaaa agccattaaa taattttcgt
    11161 tatattgtct attattgcca tacaaagata aaaaagaata aacaaaaaga aactgtgaca
    11221 aaagtcttgt cacagtccct tatctga
//
LOCUS       DACIRY010000090        11121 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.092, whole genome shotgun sequence.
ACCESSION   DACIRY010000090 DACIRY010000000
VERSION     DACIRY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.092"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            104..340
                     /gene="secG"
                     /locus_tag="IYE22_001971"
     CDS             104..340
                     /gene="secG"
                     /locus_tag="IYE22_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ9507176.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            452..1198
                     /locus_tag="IYE22_001972"
     CDS             452..1198
                     /locus_tag="IYE22_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ9507177.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            1201..3558
                     /gene="rnr"
                     /locus_tag="IYE22_001973"
     CDS             1201..3558
                     /gene="rnr"
                     /locus_tag="IYE22_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ9507178.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            3580..4044
                     /gene="smpB"
                     /locus_tag="IYE22_001974"
     CDS             3580..4044
                     /gene="smpB"
                     /locus_tag="IYE22_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ9507179.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            4346..5065
                     /gene="atpB"
                     /locus_tag="IYE22_001975"
     CDS             4346..5065
                     /gene="atpB"
                     /locus_tag="IYE22_001975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ9507180.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            5118..5333
                     /gene="atpE"
                     /locus_tag="IYE22_001976"
     CDS             5118..5333
                     /gene="atpE"
                     /locus_tag="IYE22_001976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ9507181.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            5424..5948
                     /gene="atpF"
                     /locus_tag="IYE22_001977"
     CDS             5424..5948
                     /gene="atpF"
                     /locus_tag="IYE22_001977"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ9507182.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            5935..6477
                     /locus_tag="IYE22_001978"
     CDS             5935..6477
                     /locus_tag="IYE22_001978"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ9507183.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            6500..8056
                     /locus_tag="IYE22_001979"
     CDS             6500..8056
                     /locus_tag="IYE22_001979"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ9507184.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            8070..8972
                     /locus_tag="IYE22_001980"
     CDS             8070..8972
                     /locus_tag="IYE22_001980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ9507185.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            8995..10401
                     /gene="atpD"
                     /locus_tag="IYE22_001981"
     CDS             8995..10401
                     /gene="atpD"
                     /locus_tag="IYE22_001981"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ9507186.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            10415..10837
                     /locus_tag="IYE22_001982"
     CDS             10415..10837
                     /locus_tag="IYE22_001982"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ9507187.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         3685 a         1982 c         2456 g         2998 t
ORIGIN      
        1 agttttgaac tttcagctgt tattccattt atatagagtt gtcacggact tttatctatg
       61 ataaagttaa agagacgata tcttttggga aggaggctgc gatatgtata atttaatttt
      121 gggaatcgtg atcgttttat cgattgttat ggtcattgcg attatgatgc agccaagcaa
      181 gcaaaatagt gcagcgagtg catttaccgg cggtgcggat caactattcg gcaaacaaaa
      241 agctcgcggc tttgaagctg tcatgcagcg atcaactgca gttatgggag ctatctggat
      301 gatcttatta tttgtcctag catatttatc ttcgaagtag ttgaataagc ctctcgcaaa
      361 acggcgggcg gcttttttta ttgcaaagag aacgcttaag aagaagttat gaaagaaact
      421 atggtaaaat gaggcgaaag aggtgagacg agtgagacaa ttaccaaaac ccctttatgc
      481 aaaacatgga aagcgagcgg ttttgctgct tcacgcttac tcaggaagtc caaacgatgt
      541 tcgaatgctc gcacgatttt tggaaaaagc agattataca gtttatgcgc cgctttttaa
      601 aggacacgga acaatggagc catatgatat cttgcaagaa aaagcagaaa gctggtgggc
      661 agacactaaa aaagccatcc attttttaca gtctgagcag ttttctgata ttgccgtatt
      721 aggattatca atgggcggca tctttgctgt acgagcacta gaagaagaat cagtgattgg
      781 cggcggtttt ttctgttcgc ctttgtctcc tgtaaaaaca aacgtgcctg aaaactttga
      841 aaaatacgta cgtcaagtgc tgaagacagc aggtaaatca gagaaagaaa tcaatgaaaa
      901 agcagttgct taccgctcgt tagctgaaca acaattaatg gatatacaag atcaggcagc
      961 aatcgtagaa agtaggttgt cagatatcca acagccgata tttttagctc aagctgggaa
     1021 agatgaaatg atcgacccta atggcgtatt tgaaactgca cgcaagttga gccgtcagcg
     1081 agtgacactt caatggtatc ctgaaagtgg tcatgtgatt acagttggta cagcaagacg
     1141 cgaattagaa aaagacgtct tggaattttt agaaaaatta ccttggaatg aggaataaat
     1201 atgacaaaag aaacgataaa agagaaaatt ctctttttca tggaaagcag tcgcaaacgc
     1261 agtttttcta tggaagaaat cgctgaaggt ttagggttcc aaaaaagcga tgattttaaa
     1321 ttactagtac aaacgattgc tcaaatggaa agagaacaga gtatagtttt caataaaaaa
     1381 ggaaaagtga aacttccttt aaaaccaatc ttgatcgaag gaacatttcg agcaaatgaa
     1441 cgaggatttg gatttgtaac gattgatccc gaagaagatg acatctatat cccaaaagaa
     1501 gcgactggtt ttgcaatgga tggagacacc gtagcaatcg atatcatgaa gcgagcaaat
     1561 acagatatgg atcgcggagc agaggggaaa gtagtcgaga ttcgagaccg ggcgactact
     1621 cagttagcag gggaattcgt tgcttattca gaagaagaaa tacaagaaac agatctttac
     1681 ggcgttgtta tcccgaaaga tagaaagctc aaccaattta aagtatatgc agcagcagag
     1741 ggagttcgtc cagttgacgg aagtattgtg ctgatcgaac tgacccatta ccctgaaaaa
     1801 gggtatgcga ctagtttaga agggataatc aagcaagtga ttggtcataa gaatgaccct
     1861 ggcatggata tcctctcaat tgtcgtggct aatggtatcc ctacgaaatt tcctgatgat
     1921 gtattgtctg aagcagatga agttccagat caaatcagcg aaaaggatat aactggacgt
     1981 agagatttaa gaaatcagct cattgttacg attgatggag aagatgcaaa agatctagat
     2041 gatgcagtta cggttaaaaa attagataac ggtaattatt ttcttggagt acacattgca
     2101 gatgtttctt actatgtgac acaaggcagt caattggaca gagaagcata tgaaagaggg
     2161 acaagtgtat atttgactga tcgagtgatt ccgatgatcc cgcaacgttt atcgaatggt
     2221 atctgttcac tgaatcccca tgttccaagg ctgaccatga gttgcgaaat ggagattgat
     2281 ccaaatggac aagtagtctc tcatgatatt ttcccaagtg tgatccaaac aacagaacgg
     2341 atgacctaca cagctgtcaa tcaaatctta gaagatcaag acgaagaaac gatgcaacgc
     2401 tatgctcagc tcgtacctat gttccaagaa atgggtgaat tgcatcgaat ccttgaaacg
     2461 atgcgaatca aacgtggcgc gatttctttt gaggatcgcg aagcgaaaat ccttgttgat
     2521 gctgaaggac acccaaatga catccagcta agaagcagag gaattggtga acgcttgatc
     2581 gaatctttca tgcttgcggc caatgaaacg gtagctgaac attataataa attaaatctg
     2641 ccgtttattt accgaatcca tgaacagcca aaagaagaaa aaatgcagcg cttctttgat
     2701 tttgcttcag ctttgggcat tctggtccga ggaacgaaag gaacgatcac accaaaagat
     2761 ttacagcatg tgatcgaagg agtagaagac aaaccagaag ctgcagttat caatactatg
     2821 ttgctaagaa gcatgcaaca agcacgctat tcagaagaca attttgggca ttatggctta
     2881 gcggcagatt actacacaca ctttacatct cctattcgac gctatcccga tctaatcgtc
     2941 catcgtctga tccgttctta cagtgaagat aaaagtgaaa gcaatcaaag ttcgtgggct
     3001 gcggaacttc ctgaaattgc agatcatagt tctaagatgg aacgtcgtgc agtagaaaca
     3061 gaacgtgaag tagattccat gaagaaagca gaattcatga tggacaaagt gggagaagaa
     3121 ttcgatggga tcatcagctc tgttgtgaaa ttcggcctgt ttatcgaact gccgaataca
     3181 gtagaaggat tgatccatat caatgaattg aaacaggact acttccattt tgttgaaaat
     3241 catttagctc ttgtaggtga aagaactggt ctgactttta aaatcggaca aaaagtgcgc
     3301 gtaaaagtag tcaaagctga tccagatgaa cgagcagtgg attttgaact cgtttccgca
     3361 gaagaggtca ctgcgttgga acgtccaaaa gcacgcaatg gaaaatctgg aaaacgagaa
     3421 ggaaaacaag caggcaaacg ctcagaaaaa aatggcaaga agaacaaacg atatagtgat
     3481 agtaagcaaa cgttcggtaa gaaaaaaggg aaaaagccat tttacaaaga tgtcaaaaag
     3541 aagaaaaaga aaaaataaaa tgagttggag gaaacagcta tgccaaaagg cgagggaaaa
     3601 ctaattgcac aaaacaagaa agctcgccat gattattcga tcatcgacac gatggaagca
     3661 gggatggtct tgcaaggaac ggagatcaag tcgatacgaa acagccggat caatctgaaa
     3721 gatggattta ttcgcgtccg caacggggaa gctttcttgc ataatgttca tatcagtcct
     3781 tatgaacaag gaaatatttt taatcatgat ccgttgcgca cgagaaagtt attattacac
     3841 aagaaacaaa tcgttcgtct tgaaaacgaa ttgaaaaaca ctggaatcac tgttgttcct
     3901 ttaaaagtct atattcgtaa cggctatgcc aaggtattga ttggtctggc aaaagggaaa
     3961 aaatcgtatg ataaacggga agatttgaaa cgaaaagata tcgatcgaca aattgatcga
     4021 acattaaaaa atttctctag ataatttcat tatccttttt cgcatattcg gtgcttttgc
     4081 ttttacttaa tgataaaatt tgttagcatt taatctgata aaacatgatt atcacaggtt
     4141 ttttcttaga tcaggagcac cgtgcatggg gaaaaattcg cttcgttttt gactgaaata
     4201 aaaaagtgtc taattttcat gaaaatgttt tgaattttct gtttcatggt ggtatgatac
     4261 cgtgtggtta gaaaccgatt ggaaaaaacc agatacgcac atgaattgat tttatgtgaa
     4321 tttcggggaa aagaggtgaa atggattgga tgaacgctcg ctaacgtttc acattggacc
     4381 aatttggttt gatggtacag tttgtataat ggtgttactg acctgtttga ttgttttctt
     4441 cttagcttac ttttttacaa gaaatcttaa gttgaaacca acaggcaaac aaaatgcgat
     4501 agaatgggtc attgatttta gtcgaggaat cgtaacagat aatctgccta gaaaagaatt
     4561 gagcaatttc catttgttgg catttacatt gtttttattt gtttttgttg caaacaacat
     4621 aggtttagta acaaaaatcg tcttacctaa cgaaacaaca ctttggaaaa gcccgacagc
     4681 agacccgttt gttactttaa ctttggcatt tataatgatc ttacttactc acttattcgg
     4741 ggtgaaaaag ttaggcttca aaggatattt cgtaaattcg ttcttgaaac cctatagttt
     4801 tatgtttccg atgaaactga ttgaagaatt tacaaattta ttaacacttg ctttacgtct
     4861 ttatgggaat atttatgcgg gggaagtttt gctaaccttg attgcaaaaa tgatgacaag
     4921 cttaggctgg ttctcattac cattagcaat tccattagag atggtctgga tcgcgttctc
     4981 cttgtttatt gggagcatcc aagcatttgt ctttgtcact ttgtcaatgg tttatatgag
     5041 ccataagatt gaagtagaag aataatactc ggttaaaaaa caaactacta tttatctatt
     5101 ttaggaggaa aacaattatg aattatatcg cagcagcaat cgcaatcatg ggagcagcaa
     5161 tcggagccgg ttatggtaac ggtcaagtta tctctaaaac aatcgaatca atggctcgcc
     5221 aacctgaaat gtctggtcaa ttccgtacaa caatgtttat cggggtagct ctagttgaag
     5281 ctgttcctat cttaggggta gttatcgcct tgattctagt ttttgccgtt taattgaatc
     5341 agtattaagg cagaaacaac atcgtttgtt actgcttttt tacatggaaa ctagaaatac
     5401 tccaaagaaa ggaaggctcg cctatgctga atcaattggc aattgcagaa gttggcaatg
     5461 cgatgttagg taatatcatc gttgtcagcg gctcattttt gatactactg gctctcttga
     5521 agcactttgc ttggggacca atcagcgata ttttgaaaaa acgtgaagac aagatcgcca
     5581 atgatttaga ttctgcagaa caatctcgca tcaactcagc gaaaatggaa caagaacgcg
     5641 aacaacaatt gttagcctct cgttctgatg cagctgatat catcaaaaat gcgaaagaaa
     5701 gtggagaatt aagccgccaa aatattttga aggatgctca agaagaagca gctcgtctaa
     5761 aaagcaaagc ccaagctgat atcactgtag aacgtgattc agcgctgaac tctgtaaaag
     5821 acgacgttgc agaactctct cttcaaatcg cggaaaaaat cttgaacaaa gaattatctc
     5881 cagaaatgca tgaatcatta atcaatcaat acattgaagg tctaggttct tcaaatgaaa
     5941 ctagataaat atacagtagg aagacgttac gggaaagctt tatttgaact ggcgatcgat
     6001 tcaaatcaag cagaagaaat ctatcaagat ctcgtaagtt tacgtcaaat ctatgaacaa
     6061 gtacctggat tagggaatat gttaagtgat gttcgtctag aacctcatga aaaacgagaa
     6121 atcatggaca agcttgtcag cggttatgat gggatcgtta aaaacttttt agaagttgtt
     6181 tatgagtata accgaatgga tgaccttttg ttcatgatcg aagaatatga acgtcgatat
     6241 gatgaacatc aagggttact attgggaagt gtcacaacag cagtcccact ttcagacgaa
     6301 caacgaatac aattagaaaa gaatgttgca aaaacaatgg actatcaatc agttgaatta
     6361 aaacaaatcg ttgattcttc tattattggt ggcgcgattg ttgaagccaa ccatcgagta
     6421 atcgacggaa gcattcgcac gcaattagaa aaaatgcgta atcaattaaa cagatagtac
     6481 aaagaggtag gtgaatcgaa tggccatcaa agcagaagaa attagtgcct tgattaaaga
     6541 acaaattgaa aattatcaaa acgtgctttc agtcgaagaa atcggtaccg taacttatgt
     6601 aggtgacggg atcgctcgtg cacatggttt agaaaacgcg atgagtggtg agttgcttga
     6661 attttcaaac ggctcatacg gaatggcgca aaacttagag tcaaatgatg taggtatcat
     6721 tatccttggc gatttcgagt ccattcgtga aggagataaa gtaaaacgaa caggtaagat
     6781 catggaagtt ccagttggag aggccttgat cggtcgggta gtcaatccgc taggtcaacc
     6841 aatcgacgga ctaggtgaaa tcgttactga caaagctcgt ccagtagaag cgatggcacc
     6901 aggtgttatg caacgtaaat ctgttaacga accaatgcaa acagggctaa aagcgatcga
     6961 cgcccttgtg ccaatcggac gcggacaacg tgaattagtc atcggtgacc gtaaaacagg
     7021 gaaaacttct attgcgatcg atacgatcat caaccaaaaa ggtcaagata tgatctgtat
     7081 ctatgtagca atcggacaaa aagattctac agttcgtaca caagttgaaa cattgaaaaa
     7141 atatggcgca atggattaca caatcgttgt gaatgccggt gcgtctcaac cagcaccatt
     7201 gctttatatc gcaccatatg ctggtactgc aatgggtgaa gaattcatgt acaacggtaa
     7261 acatgtattg atcatttttg atgatctttc aaaacaagct gttgcttatc gtgaactttc
     7321 cttgctactt cgccgtccac caggtcgtga agcttatcct ggggatgttt tctacttgca
     7381 ctcacgttta ttagaacgtg cagcaaaatt aagtgatgat ttaggtggcg gttcaatgac
     7441 tgccttacca tttgttgaaa cacaagctgg agatatctct gcttatatcc caacaaacgt
     7501 tatttcgatc actgatggac aaatcttctt agaaagcgac ttgttctacg caggtactcg
     7561 tccagcggtt gatgccggac tatctgtatc tcgtgtaggg ggttctgcgc aaatcaaagc
     7621 aatgaaaaaa gttgctggga ctttacgcct agacttagca agttaccgtg aattagaagc
     7681 atttacacaa ttcggttctg acttggatgc agcaacacaa gcaaaactaa atcgtggacg
     7741 ccgtacagta gaaatcttga aacaaaaatt gcatgcaccg ctagctgttg aaaaacaagt
     7801 agtgattttg tatgctttga cacatggatt cttagatagt atccctgtag acagtatttt
     7861 agacttcgaa catgaattat tcgaatatct ggataccaat cattcagata tctttgaaac
     7921 aatccgtaca acaaaagatc ttccagaaga agaagcgttg aacagtgcga ttcaggaata
     7981 taaagacatg ttcttagcta caaaaggaag taattcctct acagaagata aattgaaatc
     8041 cattcaaaat gcatagtgag gtgagatgaa tgggtgcttc attaaatgaa atcaagacac
     8101 gtatcgcttc aacgaaaaaa acgagccaaa tcacgcgggc aatgcagatg gtgtctgctt
     8161 ctaagttgac aaaatctgaa gcttcttcac agaagtttca ggtttatgca acaaaagtaa
     8221 gagaaatcgt tacgcatttg actgctactc agttaaatga tattgcctca gaaaatccta
     8281 gaggggatat caattacaac agcatgctgc tcagccgtgc tgtgaagaaa actggttata
     8341 tcgtcatcac tgcagatggc ggtttagtcg gtggatataa cagctcgata ttgaaacaaa
     8401 cgatgtctat tttagaagaa gaccaccaat cacccgatga ttacgtgatg atcgcaattg
     8461 gcggaactgg tgcagacttc tttaaagcac gcggtatcaa tctagcatat gagctgcgca
     8521 atctatctga tcagccaagc tttgatgaag tccgcaaaat cgtaaatatg gcaacaacta
     8581 tgttccaaaa cgaagtattc gatgaattat acgtatgtta taaccatcat atcaattcgc
     8641 taactagtca gtttcgtgta gaaaaaatgc tgccaatctc agacttagat ccagaagaag
     8701 caacgacttt cgaacaagaa tatatttttg aaccttcaaa agaagaaatc ttgtctcagt
     8761 tattgcctca gtatgctgaa agtttgatct acggtgcgat cgttgacgcc aaaacagcag
     8821 aacatgctgc tggtatgaca gcaatgaaaa cagcaactga taatgctgct aatatcattg
     8881 atgagctgac ggtttcttat aaccgtgcaa gacaaggggc tattacccaa gaaattaccg
     8941 aaattgttgc cggagcttcg gcactagaat agtattgaga ttggaggaaa aaagatgagt
     9001 tcaggcaaga ttgttcaagt aatcggtccc gttgtcgacg tggaattttc tttagaccaa
     9061 tccttaccag atatcaacaa cgcattagtt gtttataaaa atgacgaaaa taaatcaaaa
     9121 gttgttcttg aagcagcgtt agaattaggg gacggagtga tccggaccat tgcgatggaa
     9181 tcaacagatg gtttacaaag aggaatggaa gtcattgata caggcaaagc aatctctgtt
     9241 cctgtaggta aagaaacatt aggtcgcgta ttcaacgtac taggagatac gatcgattta
     9301 gaaacacctt tcccagaaga tgcggaaaga agcgaaattc ataaaaaagc accagccttt
     9361 gacgaattaa gtacaagtac agaaattttg gaaacaggga tcaaagttat cgatttgctt
     9421 gccccatatt taaaaggtgg gaaagtcgga ctattcggtg gtgccggtgt tggtaaaacc
     9481 gtactgatcc aagaactgat ccataatatc gcccaagaac atggtggtat ttctgtattt
     9541 accggtgtag gtgaacgtac tcgtgaaggt aatgacttgt attatgaaat gaaagattca
     9601 ggagttatcg aaaaaacagc catggtgttc ggacaaatga acgaaccacc aggtgcacgt
     9661 atgcgtgttg ctttgactgg tttgacgatt gcggaatatt tccgtgatgt agaaggtcaa
     9721 gatgtactgt tgtttatcga caacattttc cgtttcactc aagctggatc tgaagtatca
     9781 gccttgttag gacggatgcc ttctgcggtt ggttatcaac caacattggc aacagaaatg
     9841 ggtcaattac aagaacgtat cacatctacg aaaaaaggtt cgatcacatc gatccaagca
     9901 atctatgtgc cagccgatga ctacactgac ccggcgccgg caacagcatt cgcccatttg
     9961 gatgcgacaa ccaacttgga acgtaaatta acagaacaag ggatttatcc agcggtagat
    10021 ccattggctt cttcttcaag tgctttagca cctgaaatcg tgggggaaga acattataaa
    10081 gtagcaacag aagtccaaca cgtattgcaa cgatatcgtg aattacaaga tatcattgct
    10141 atcttaggta tggatgaatt atccgatcaa gaaaaagtgt tagtttctcg tgcccgcaga
    10201 gtccaattct tcttgtcaca aaactttaac gtggctgaac aatttactgg tcttccaggt
    10261 tcatatgtcc ctgtagaaga aacagtgaaa ggattccgcg aaatcttgga aggcaaatac
    10321 gatgatcttc ctgaagaagc tttccgaagt gttggccgaa tcgaagacgt cgtagaaaaa
    10381 gcgaaaacac taggctacta gaaaggggtg ccccatggat caatatttaa cagtaaatgt
    10441 ggtaactcct gccggtttgg tctatgatca ccatgcagct atcgtcgttg cacgtacgac
    10501 agctggtgag cttgggattt tgcctaaaca tgcaccaatc atcgttccat tgacgattga
    10561 tgaagttcgt gtaaaacgaa cagattcaga tacccacgtc gattggatcg ctgttaatgg
    10621 cggaatcatg gaagttcgtg ataatgttgt ttcaatcgtt gctgatagtg cggaacgtga
    10681 acgtgatatc gatgtatctc gtgcggaacg tgcaaaacaa cgcgcagaac gccaaatcga
    10741 agaagcaaaa gcaaaacaag atacgaacga attgaaacgg gcaactgttg cgttgcaccg
    10801 agcaatcaat cgtatcaaag tatctaagca ctcataaaat gagatgcgga tgtttatccg
    10861 tgtttaagaa cagtaaaaag aggttatgac agaagtggtc agcttcaaga cataagaagg
    10921 aatttacgaa aattgcttct caaatttttg tgaaatctca gcttattttt cgaagaagct
    10981 acttctggaa caccgtatat cagaggttgt aaaatcagcg ttttgtcctg agtattagag
    11041 aaacaaatgg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    11101 atgattgaac accgtttatt t
//
LOCUS       DACIRY010000091        11047 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.093, whole genome shotgun sequence.
ACCESSION   DACIRY010000091 DACIRY010000000
VERSION     DACIRY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.093"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            99..1046
                     /locus_tag="IYE22_001983"
     CDS             99..1046
                     /locus_tag="IYE22_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9507188.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            1176..1916
                     /locus_tag="IYE22_001984"
     CDS             1176..1916
                     /locus_tag="IYE22_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507189.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(2207..2875)
                     /locus_tag="IYE22_001985"
     CDS             complement(2207..2875)
                     /locus_tag="IYE22_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9507190.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            3009..3296
                     /locus_tag="IYE22_001986"
     CDS             3009..3296
                     /locus_tag="IYE22_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507191.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            3712..5250
                     /locus_tag="IYE22_001987"
     CDS             3712..5250
                     /locus_tag="IYE22_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ9507192.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            5247..6212
                     /locus_tag="IYE22_001988"
     CDS             5247..6212
                     /locus_tag="IYE22_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507193.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            6199..7521
                     /locus_tag="IYE22_001989"
     CDS             6199..7521
                     /locus_tag="IYE22_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9507194.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      7584..7688
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7762..8505
                     /locus_tag="IYE22_001990"
     CDS             7762..8505
                     /locus_tag="IYE22_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9507195.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            8520..9512
                     /locus_tag="IYE22_001991"
     CDS             8520..9512
                     /locus_tag="IYE22_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9507196.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            9524..10261
                     /locus_tag="IYE22_001992"
     CDS             9524..10261
                     /locus_tag="IYE22_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507197.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            10251..10973
                     /locus_tag="IYE22_001993"
     CDS             10251..10973
                     /locus_tag="IYE22_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ9507198.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         3721 a         1863 c         2308 g         3155 t
ORIGIN      
        1 ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt tggtataatt
       61 gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta gcagatgttg
      121 caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat ccggaacagg
      181 tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat taccgtccaa
      241 atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta tttatactgg
      301 aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc gctaaacgga
      361 tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac gtcggttctt
      421 gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc gaccgtttgg
      481 aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta gacagcgata
      541 atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat caaacgatta
      601 tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact ggttatttat
      661 ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat cattcgacag
      721 aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact tgttttgtat
      781 gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct ggaggacact
      841 tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa atcagttcac
      901 ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt ggagaaatgt
      961 tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt tttcctgaac
     1021 tagttatcag agagacaacg aattaagtct tattactata tatatattta tgaaattcat
     1081 cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct gtgctattat
     1141 tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga cgaaaacatt
     1201 tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac aagaagcgag
     1261 tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg tttgaacaga
     1321 tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag cgtcgtgcaa
     1381 caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa ggaaaattac
     1441 cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa aaactttttg
     1501 gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag catactacac
     1561 gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct aaatatgtac
     1621 ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat ttaatcgaat
     1681 cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg ccgtatcaag
     1741 aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg ttagaggaaa
     1801 caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa cgctcattac
     1861 aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt aaataagtga
     1921 tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt ccttatttta
     1981 tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag cgaatttttt
     2041 ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactattttc gcagatcgtt
     2101 tgcaaaaata gtttattttt ttataaagaa gattttactg aataatatca ttttagttta
     2161 cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact
     2221 gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca
     2281 aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc
     2341 taagtaatgt atacagaaaa tgtgtgaggt ccaaataaag caaggagaca actaaaatac
     2401 caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt
     2461 gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa
     2521 tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta
     2581 tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc
     2641 ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa
     2701 gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag
     2761 taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga
     2821 ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta
     2881 gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact
     2941 tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga
     3001 gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag
     3061 agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga
     3121 taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga
     3181 aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag
     3241 aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa
     3301 aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa
     3361 aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg
     3421 agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag
     3481 atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa
     3541 cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta
     3601 gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg
     3661 gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat
     3721 tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg
     3781 tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg
     3841 gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg
     3901 attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca
     3961 gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt
     4021 ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg
     4081 agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga
     4141 actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga
     4201 ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt
     4261 gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat
     4321 aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt
     4381 tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga
     4441 gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca
     4501 gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa
     4561 ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta
     4621 cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa
     4681 ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat
     4741 gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg
     4801 gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat
     4861 ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag
     4921 ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc
     4981 acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc
     5041 ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc
     5101 gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat
     5161 taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat
     5221 gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa
     5281 aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa
     5341 tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg
     5401 gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata
     5461 atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga
     5521 ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga
     5581 aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc
     5641 gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag
     5701 ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga
     5761 tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca
     5821 tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta
     5881 tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa
     5941 acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag
     6001 atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt
     6061 gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga
     6121 tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa
     6181 gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc
     6241 ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa
     6301 gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt
     6361 gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca
     6421 actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa
     6481 agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt
     6541 ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg
     6601 gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt
     6661 gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat
     6721 attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat
     6781 cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa
     6841 atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa
     6901 ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt
     6961 gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt
     7021 gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg
     7081 tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga
     7141 aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata
     7201 cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc
     7261 tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc
     7321 aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct
     7381 gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa
     7441 aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat
     7501 ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct
     7561 tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt
     7621 aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag
     7681 cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat
     7741 tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc
     7801 attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc
     7861 ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct
     7921 gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt
     7981 actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg
     8041 atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc
     8101 ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct
     8161 ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg
     8221 gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt
     8281 tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta
     8341 agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat
     8401 aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt
     8461 tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa
     8521 tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa
     8581 aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt
     8641 atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag
     8701 aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag
     8761 taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg
     8821 cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg
     8881 gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat
     8941 atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg
     9001 gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag
     9061 gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag
     9121 cgggtattga actaaactat atcccattga aagaattaga tgaacggtta gattactata
     9181 ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt
     9241 ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta
     9301 aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat
     9361 tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt
     9421 ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc
     9481 aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag
     9541 ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc
     9601 taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa
     9661 aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt
     9721 aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct
     9781 gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga
     9841 acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga
     9901 acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc
     9961 ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat
    10021 gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca
    10081 tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc
    10141 acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca
    10201 gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg
    10261 atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga
    10321 ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag
    10381 caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt
    10441 ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata
    10501 atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt
    10561 ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct
    10621 taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact
    10681 atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga
    10741 atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg
    10801 atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct
    10861 cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag
    10921 ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag
    10981 atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt
    11041 gattaat
//
LOCUS       DACIRY010000092        10504 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.094, whole genome shotgun sequence.
ACCESSION   DACIRY010000092 DACIRY010000000
VERSION     DACIRY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.094"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            169..2403
                     /gene="pcrA"
                     /locus_tag="IYE22_001994"
     CDS             169..2403
                     /gene="pcrA"
                     /locus_tag="IYE22_001994"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ9507199.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2420..4456
                     /gene="ligA"
                     /locus_tag="IYE22_001995"
     CDS             2420..4456
                     /gene="ligA"
                     /locus_tag="IYE22_001995"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ9507200.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            4562..4867
                     /gene="gatC"
                     /locus_tag="IYE22_001996"
     CDS             4562..4867
                     /gene="gatC"
                     /locus_tag="IYE22_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ9507201.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            4867..6339
                     /gene="gatA"
                     /locus_tag="IYE22_001997"
     CDS             4867..6339
                     /gene="gatA"
                     /locus_tag="IYE22_001997"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ9507202.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            6336..7766
                     /gene="gatB"
                     /locus_tag="IYE22_001998"
     CDS             6336..7766
                     /gene="gatB"
                     /locus_tag="IYE22_001998"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ9507203.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            7776..8831
                     /locus_tag="IYE22_001999"
     CDS             7776..8831
                     /locus_tag="IYE22_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ9507204.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            8942..10315
                     /gene="rlmD"
                     /locus_tag="IYE22_002000"
     CDS             8942..10315
                     /gene="rlmD"
                     /locus_tag="IYE22_002000"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ9507205.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            10318..10446
                     /locus_tag="IYE22_002001"
     CDS             10318..10446
                     /locus_tag="IYE22_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ9507206.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         3648 a         1893 c         2324 g         2639 t
ORIGIN      
        1 cctttttttt ccattcaaac gcctcgcttt cttttaattt agcgcgaaat caacgaattg
       61 acaaggaaaa atccataaaa agaaacaaat gattttgaac tagctagttt aatgaaagcc
      121 aagaaaatgc tataattaac aagattgata aaagaaagga tgttgttaat gtcatccaaa
      181 gctgaattat taaacgggat gaacccccgt cagaaagaag ctgttcttca cactgacgga
      241 ccattactgc taatggcagg tgcaggaagt ggaaagacga gagtgctgac acaccgcatt
      301 gcctatttga ttgaggaaaa agaagtcaat ccttggaaca tcctagcgat tacttttacc
      361 aataaggctg caaaagaaat gaaagaacgt gtcaatgcga ttctcgcatc tggaggcgaa
      421 gatgtctggg tatcgacttt tcactcgatg tgtgtgcgta ttttaagaag agatgttgat
      481 tttatcgggt ataaccggaa ctttacaatc atcgattctt ctgaacagct gaccttgatg
      541 aaacggatac taaaagaact gaacattgat ccgaaaaaat atgatcctcg aagtatatta
      601 ggaacaatct ctcaagcaaa aaacagcctg cagacccctc aggattttgc aaaaatgcaa
      661 ggcagttatt acgaagagat tgcggcaaaa tgttatgctg cttaccaaaa agagcttcaa
      721 tataatcaat gtatggactt tgacgacctg atcatgaata cgattcgatt atttgaagaa
      781 catcctgatt cactcacata ttatcaaaac aaattccatt atattcatgt agatgagtac
      841 caagatacaa atcacgcaca gtatacgcta gtcaatttat tggcaggtag atttaggaac
      901 ctgtgcgtcg tgggagatgc agatcaaagt atttacggct ggcgcggtgc agatatgcag
      961 aacatcttgg attttgaaaa agattatcca gatgctgcag taattttgct agaacaaaac
     1021 tatcgctcca cgaaaaacat ccttagcgca gcaaaccaag tcattgaaaa caatagcaat
     1081 cgaaaaccaa aaaacttgtg gacagaaaac aaagaaggaa acaagatcac ttactaccgt
     1141 gcagataatg aacgagacga aacccgtttt atcgtagatc ggatgcaaga agagattcga
     1201 agcaatcatc ggaattacgg cgactttgcc atcttgtacc ggacgaacgc acaatctcgt
     1261 gtaatggaag aaactctgtt gaaagcaaat attccttata aaatggttgg agggcacaag
     1321 ttctacgatc gtaaagaaat caaagatatt ttagcgtatt tgaatgtgct ggcaaatcct
     1381 caagattcaa tcagttttga gcgtatcgtt aattcgccca aacgaggcat cggaccagga
     1441 tctatcgaaa aactacgcag ttttgcttct cttcatgaat ggccactgct tgaagcagcg
     1501 caaaatgtgg atctggcaaa tatcggtgga aaagcaggac agcaattagg tgcatttgga
     1561 gagatgatcc aagaagtcac tcagatgatc ccatacttga cagtgacaga attgacgaaa
     1621 gaggttttgg atcgtagcgg atatctagaa gacttaaaaa tccaaaatac attagaagca
     1681 caagcacgca tcgaaaactt ggaggaattt ttaacggtca cacaagaatt cgacaaacaa
     1741 ttcgaacagc aaaatgaaga agacgctgat gcaccagaag aaaaactaac ggtcttctta
     1801 aatgatctgg cacttgtttc cgatattgac aatttagaag aagatgcttc ccaagtgaca
     1861 ttgatgactt tgcatgcagc caaaggatta gaattcccag ttgtattcct gattggtcta
     1921 gaagaggggg tttttccttt atctcgagca ttgatggaag aaagcgaact ggaagaagaa
     1981 cgccgtctcg catatgtggg gatcacgcga gcagaagaag cgttgtattt aacaaatgct
     2041 ttctccagaa cattatatgg acggacgcaa tataaccgac cttctcgttt tgtagaagaa
     2101 atcgatcagg agttactgga aatcgaaggc atgcgtccta cgcctaaaaa aacacctgta
     2161 ttcgctaaaa aaacagcata tagctataaa cagccagaga cagcggttgt tccctctaaa
     2221 agtgctactg gaggggaaaa taacagctgg aaacctggag ataaagtcaa acacaaaaaa
     2281 tggggtctag gaacagtcgt ccgagtaagc gggacgtcga aggatctaga actagatgtg
     2341 gctttcccaa gtcaaggagt aaaacgtctt ttggctgcct ttgcgccaat tgaaaaagct
     2401 tagtagtgga gggtaaatga tggaacatga aatgacgatt gacgaagctg ctaaacgagc
     2461 agaagaactt cgtacaagac tgaaccaatg gtctcgcgaa tactacgtag aagataaacc
     2521 aacagtggaa gactatgttt atgataaaga atacgcagaa ttagtagcaa ttgaggaaca
     2581 atatcctgat ttgatcacgt ctgattctcc cactcaacga gtaggcggaa aagtattaga
     2641 aggttttgaa aaagtcactc atgatattcc gctttacagt ttgaacgacg ttttcagcaa
     2701 agaagagctg atagcttttg atcaacgtgt acaaaaagca gtgggacgtg tagttgacta
     2761 ttgctgtgaa ctaaaaatag atggactttc cgtttctttg cgttatgaag atggaaattt
     2821 cgtgagaggt gcgactcgcg gggatggaac tgtaggagaa aacatcactg aaaacctaaa
     2881 aactgtccgg tcggtcccaa tcaaattaaa agaaccaatg aacatcgaag taagaggcga
     2941 atgtttcatg ccaaaacggt catttgtcca attgaaccaa gacagagaag cagagggaaa
     3001 agatatcttt gcgaatcctc ggaacgccgc agctggttct ttgcgtcagt tagattctaa
     3061 aatcacagcg aaaagaaatc tggacacttt tttatacaca gtagctgatt tcggtcctat
     3121 gcaagcaaaa acccagtatg atgcgctaga agagctagaa aaaattggat tccacacgaa
     3181 tcgggaaaaa cgtctatgtc attcgattga tgaagtgtgg tcatacattg aagaatatca
     3241 tgacaaacgt gtggatttgc cctatgaaat cgatggaatc gtcatcaaag tgaatgaatt
     3301 ctcgctacaa gatcaacttg gttttactgt caaagcaccg agatgggcag cagcttacaa
     3361 gtttccgcca gaagaagttg aaacgctaat cgaaaacatc gaatggacag tcggtcgaac
     3421 aggggtagta acgccaacag ctatcatgac accagtccga gtagcaggaa cgacagtaag
     3481 ccgtgccagc ctgcataacg gtgattatat taagttgaag gatatccgct tgaaagatac
     3541 agttctgatt tataaagcgg gagacatcat cccagaagtt tcgcaagtcg tgttagataa
     3601 acgacctaaa gacagcgaag aataccaatt gcctacacat tgtccagttt gtggaagtga
     3661 acttgtccat ttggatgaag aagtagcctt acggtgcatc aatcctaaat gccctgctca
     3721 aatgaaagaa ggtttgaacc actttgtttc aagaaatgcg atgaatatcg atgggttggg
     3781 gccgcgcgta ctagaacaga tgtatgataa aaaactagta gcagatgttg ctgatcttta
     3841 caaattgaca gaagaagagt tattgacgtt ggacaaaata aaagaaaaat cagcaaataa
     3901 tattttgaca gcaatcgaca acagcaaaga taattcggtc gagcggttga ttttcgggtt
     3961 aggaatccga catgtcggag cgaaagcagc aaaaatttta gcagaacatt tcggtgatct
     4021 agaaacattg agtaaaagtg actatgagtc gattattgcg cttgatacga tcggtgatat
     4081 cattgccgac agtgtagtca cttatttcag taacgaagaa gtccatgaat tgatgaatga
     4141 attaaaacaa gcaggcgtca atttcgaata caaagggctt cgaaatgccc aattgcaaga
     4201 agtcgagtct ccttttaaag aaaaaacagt agtccttact ggtaaactaa cacgatttac
     4261 tcgtgaagaa gcaaaagaga cgatcgaaaa tcttggcggg aaagtgactg gtagcgtatc
     4321 gaaaaaaacg gatatcgtgg tagcaggaga agatgctggc agcaagctga ccaaagcaca
     4381 agaattagga atcgaagttt ggacagaaga tcaaatggca gatgcattgg ctaaaagtcg
     4441 ctcagtagaa gaatgaaaaa tgagtttttt cagacaaatt tcaataaaca atttcagaca
     4501 cggcactctt gtgttaaaat agagagcaaa gtttttaact cgtttagaaa gaaggggtag
     4561 tatggcgatt agtgaagaac aagtgaaaca cgttgccaaa ttagcaaaac ttgcgttcgc
     4621 agatgaagaa cttgcaagtt ttaccgacca attaggtaaa atcatcgata tggttgaaat
     4681 gctggaagaa gtagatacag aaggtgttcc atttacgtcg aatgtggttg aaacagtaaa
     4741 cgtgatgcga caagatcggg cgcaagctgg atggaaccgc gatgaattat tgaaaaatgt
     4801 gccggaaaca gaagacggct ttatcaaagt acccgcaatc atcgataatg gggaggctgg
     4861 cgcataatga cagaattaca caatcgttct ctagaagaac tgcatcatat gcttgtatcg
     4921 aaagaaatca ctgtacaaga tttgacaaaa gcaacatttg aacgaatcaa agaaacagag
     4981 cctgaggtcg aagcattcat tacattgaac gaagaaaaag cattggaaca ggcaaaagct
     5041 ttagatgaaa aagggattga tgaaagcaac gtccttgctg gtattccaat tggtatcaag
     5101 gataatatcg taacaaaaga tatcttgaca accgctgcat ctaagatcct ttacaatttt
     5161 gaaccaattt atgatgcaac ggtcatggat aaaatctatc aagcagatat gatccctgtt
     5221 ggtaagctaa acatggatga atttgctatg ggtggaagta cagaaacttc ttatttcaag
     5281 aaaacaaaaa atgcttggga cccaacaaaa gtacctggtg gctcttctgg tggttctgca
     5341 gcagcagttg cagctggaca aattccagtt tctttaggta gtgatactgg gggaagtatc
     5401 cgccaaccag cagcttttaa cggcatcgta gggatgaaac ctacttatgg ccgtgtctca
     5461 cgttttggtt tgattgcctt tgcttcatct cttgaccaaa tcggaccaat gacccgtacg
     5521 gtgaaagata atgcattagc attgacagct atcagcggtt atgacgaaaa agacggtact
     5581 tcttcaggcg tttcagttcc tgattttgca gagggactga caggcgacat caaaggaatg
     5641 aagattgctt tacctaaaga atacttaggc gaaggtgtgg cccctggcgt aaaagaagct
     5701 gtattgaaag cagcagaaac attccgttca ttaggtgctg ttgtggaaga agtcagcttg
     5761 cctcattcaa aatacggtgt ggctgtttat tatatcattg cctcatcaga agcaagttcc
     5821 aacttgcaac gattcgatgg tatccgctac ggttatcgct cagaaaatgt gaaatcatta
     5881 gatgatgtat atgtcaactc acgttcagaa ggattcggtg aagaagtaaa acgccgtatc
     5941 atgttaggta cattctcctt aagtgctggt tattatgacg cttacttcaa aaaagcggga
     6001 caagtccgta cattgatcaa acgagatttt gaaaaagtct ttgcagatta tgacttgatc
     6061 atcgggccat cttcaccaac agttgcattc ggaatcggtg agaacattaa cgatccaatc
     6121 acaatgtaca tgagtgacat cttaacgatc cctgtcaact tggctggact tccaggtatg
     6181 tctgtaccgg caggtttatc agaaggatta ccagttggat tgcagatcat cggtaattat
     6241 tttgatgaaa aaacgatgta tcaagcggca tatgcgtttg aacaagcaac agaattccat
     6301 aaacaacaac cagctatttt aggagggaaa gacgaatgaa ttttgaaaca gtcattggac
     6361 tagaagtcca cgtcgagtta aaaaccaact caaaaatttt ctctccagcg ccagctcatt
     6421 ttggtgcaga accaaacagc aatacaaacg tgatcgactg gggttatcca ggcgtgctgc
     6481 cagtaatgaa taaagctgcg ctggaattcg ggatgaaagc agctttggca ttaaactgtg
     6541 aaatctctaa agatactcat tttgaccgga aaaattactt ttacccagat aatccaaaag
     6601 catatcaaat ttcccaattt gatcaaccaa tcggtcatga cggatggatc gaaatcgaag
     6661 tagaaggtaa aaagaaaaaa atccggatcg aacgtgtcca tttagaagaa gatgctggaa
     6721 aaaacatgca tggtattggc ggttattctt acgttgattt gaaccgacaa ggcacgcctt
     6781 tgatcgaaat cgtttctgaa gctgatatgc gttcaccaga agaagcatac gcttatctag
     6841 aagcattgcg atcaattatc caatttacag aagtcagcga tgtgaaaatg gaagaaggct
     6901 ctatgcgctg tgatgccaat atttctcttc gcccttatgg acaagaagag ttcggtacga
     6961 aagcagaatt gaaaaacttg aactctttat ctttcgtgaa aaaaggttta gcttttgaag
     7021 aaaaacgtca agcaaaagtt cttctttcag gaggagaaat ccaacaagaa acacgccgtt
     7081 ttgacgaaac aacgaataaa acagttttga tgcgtgtcaa agaagggtct agtgattatc
     7141 gttacttccc agagcctgat atcccgaaat tcgtgatcga tgacgaatgg attcaaaaag
     7201 taaaagctag tttgcctgag atgccagctt ctcgtcgcga acgctatatc cgtgagtttg
     7261 gacttccaga gtatgacgca atggtattga cacttacaaa agaaatgtct gatttttttg
     7321 aagctgtttt ggaaaatggt gcagatgcca agcaagcatc taactggttg atgggtgaag
     7381 tttctgctta cttgaacagt gagaaactgg aattagctga gacaaaattg acacctgaga
     7441 acttagcagg aatgattcgt ttgatcgccg atggaacgat cagctcaaaa atcgccaaaa
     7501 aagtcttccg tgaattgatc acaaatggcg gagaagccca agaagtagtt gaaaaaaatg
     7561 gttgggtgca attatctgat ccgaacaaac tactgccaat cattaatgaa attttggata
     7621 acaaccaaca atctgtcgat gatttcaaaa atggaaaaga tcgtgcggtt ggcttcctag
     7681 tcggtcaaat catgaaagca actaaaggac aagcaaatcc tggtgtcgtg aataagctac
     7741 ttaaagatga attagcgaaa cgatagaggc tgaaaatgaa aaaagcacgc gtaatttata
     7801 accccacctc gggaaaagaa ttattgaaaa aaaatttggc ggatatccta caggcattag
     7861 aagaagcagg atttgaagcc agcgcctatg caactacacc agaaccagat tcagccaaga
     7921 atgaagcatg tagagtcgca gaactaggtt ttgatctagt agtggcagct ggtggcgatg
     7981 gcacaatcaa cgaagtggtt aatggaatcg ctccgcttga acaccgtcct aaaatggcca
     8041 tcatcccagc tggtacaacg aacgactatg ctcgagcatt aaaaattcct agagataata
     8101 ttaaagcagc agcagaagtg ataaaaaaac accaaaccgt aaaaatggac atcggccttt
     8161 ctgataacag ttacttcatc aatatcgctg caggtggcta tttgacagaa ttgacttatg
     8221 aagttccatc agaactaaag agtatttttg gttatcttgc ttatcttgcg aaaggagcag
     8281 agatgctccc tcgagtaaag ccaatcaaaa tgcgcttgaa gtatgatgaa ggggaatata
     8341 tcggaaatgc ttcgatgttt ttcctagggc tgacgaactc tgttggagga ttcgaacaga
     8401 tcgcgccaga tgccaaattg gatgatggga agttttcttt aatcattgtg aagacagcaa
     8461 atatctttga gatcttgcat attgcagcac tcatgctgaa tggcggtcga catgtcgatg
     8521 atccaagaat aatctatacc aaaacaagca gtttgttcgt tgaaacagaa gatgatatca
     8581 atcgaccagt aatgatcaac ttagatggtg aatacggcgg ggaagcaccg atgcatttcc
     8641 aaaatcttca tcaacacatt gagttctttg ccaataccga tcagattcct gatgaagcaa
     8701 tcacaggtac agatgaagaa gaattagagg ttgtcagcaa agaattcgta aaagaagtcg
     8761 aacgtttaac ggatgaagat atcgacggtg acggaaaaat agcagacgaa acacctaatc
     8821 aaaagaatta atcaaaagcc ggtgctgaac gtagtaaggt cagtgccggt tttagttgtg
     8881 taattagctg atttatagta gagttcagta ggattgaaaa ataggcttaa aggagcaaaa
     8941 tatgacagaa cagcaaatag taaagaaaaa cgaacgatat acagtcgaaa tcgtcgactt
     9001 gagttatgaa ggaatggggg tagcaaaaat cgatggttat ccattattta tcgaaaatgc
     9061 actccctgga gaagaagtag aaatactagt agtaaaagca ggcaataagt ttggatatgg
     9121 aaaagtcgaa acatttatta cacaatcacc tgatcgtcag cctgtagaaa acaatgcatt
     9181 acttcgcata ggtatcgcac ctttagccca tttgaagtat gaacaacagc ttgtcttcaa
     9241 acaaaaacaa gtagagcatg taatggcgaa gattgctaag atgccagaag ttcctgtact
     9301 tccaacggtc ggaatggcag aaccattcgg ctaccgtaat aaagcacaga tccctgttcg
     9361 ccgtatcgat ggacagttag ccaccggttt ttacaaaaag aatagccatg atctagtccc
     9421 tatcgaagat ttttatatcc aagatcctgt catcgatcaa gcaatcaaag tagtcagaga
     9481 tatcttgcaa cggttccaag tacgaggata taatgaagaa aaaaatgaag gacagcttcg
     9541 tcacatcatt atccgtcgtg gtcattattc ccatgaaatg atggtcgttt tagttactcg
     9601 taaagaaaaa ttctttaaat ccaaggaaat tgctgaagct attcatgaag aattgccaga
     9661 agtggtttcg gtcatccaaa atatcaatga agaaaaaaca aatgtgattt taggagacaa
     9721 agaaaaagtt ttatatggca gagactatat cgaagatcag ctattaggaa aaacctatcg
     9781 aatctcagca aagtcatttt atcaagtaaa tactcaacaa gcagagtatc tgtaccaaac
     9841 agccatcgag tttgccgagt tagataaaaa agacattgtg atcgacgctt attcaggaat
     9901 aggtacgatt ggcttgtctt tagcagacag agtggataag gtgtatggga tggaagttat
     9961 tccagaagca attgaagatg cacagttcaa tgctttaaat aataaaatcg aaaatgttca
    10021 ttatgaagtc ggaaaagcag aaaaagttat gaaaaaatgg caagaaaaag ggatcaaacc
    10081 atctgtgatc gtagttgacc ctccacgaaa aggattagat catcatttta ttgaatcagc
    10141 aatcgaaatg tcaccagaac gaatcgtcta tatctcatgt aatccggcaa cttttgcaag
    10201 agatgctaag ttatttgcag aagcaggtta tgccgtgaag aaagttcagc ctgtagatct
    10261 tttcccgcaa acccatcata tcgaattagt cgcttcattc gttaaaggag aataaaaatg
    10321 gaccaaaaag aaaaaatgaa agaattgatc aaaaagaaac aaggtggagg ccgatccaaa
    10381 caaatggaaa cacctaagaa tgatcgtaaa aacatgagaa aaggccctaa aatctttaat
    10441 aaatagacgt gctaaagaaa gactaagaag tgttaacttt tgttattgtt tcttagtctt
    10501 ttta
//
LOCUS       DACIRY010000093        10414 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.095, whole genome shotgun sequence.
ACCESSION   DACIRY010000093 DACIRY010000000
VERSION     DACIRY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.095"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IYE22_002002"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IYE22_002002"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ9507207.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IYE22_002003"
     CDS             1007..1927
                     /locus_tag="IYE22_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9507208.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IYE22_002004"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IYE22_002004"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ9507209.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IYE22_002005"
     CDS             3233..4711
                     /locus_tag="IYE22_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ9507210.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IYE22_002006"
     CDS             4988..7048
                     /locus_tag="IYE22_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507211.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IYE22_002007"
     CDS             7131..7700
                     /locus_tag="IYE22_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507212.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="IYE22_002008"
     CDS             complement(7875..8681)
                     /locus_tag="IYE22_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507213.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="IYE22_002009"
     CDS             8819..10333
                     /locus_tag="IYE22_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ9507214.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3694 a         1739 c         2048 g         2933 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagt
//
LOCUS       DACIRY010000094        10245 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.096, whole genome shotgun sequence.
ACCESSION   DACIRY010000094 DACIRY010000000
VERSION     DACIRY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.096"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(30..686)
                     /locus_tag="IYE22_002010"
     CDS             complement(30..686)
                     /locus_tag="IYE22_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ9507215.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            879..1157
                     /locus_tag="IYE22_002011"
     CDS             879..1157
                     /locus_tag="IYE22_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507216.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1176..2384
                     /locus_tag="IYE22_002012"
     CDS             1176..2384
                     /locus_tag="IYE22_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9507217.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2532..2897)
                     /locus_tag="IYE22_002013"
     CDS             complement(2532..2897)
                     /locus_tag="IYE22_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ9507218.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3044..3817)
                     /locus_tag="IYE22_002014"
     CDS             complement(3044..3817)
                     /locus_tag="IYE22_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9507219.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3991..4851)
                     /locus_tag="IYE22_002015"
     CDS             complement(3991..4851)
                     /locus_tag="IYE22_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ9507220.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4851..6269)
                     /locus_tag="IYE22_002016"
     CDS             complement(4851..6269)
                     /locus_tag="IYE22_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9507221.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6326..6775)
                     /locus_tag="IYE22_002017"
     CDS             complement(6326..6775)
                     /locus_tag="IYE22_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9507222.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7220..7483)
                     /locus_tag="IYE22_002018"
     CDS             complement(7220..7483)
                     /locus_tag="IYE22_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ9507223.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7582..8877)
                     /locus_tag="IYE22_002019"
     CDS             complement(7582..8877)
                     /locus_tag="IYE22_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ9507224.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8874..10145)
                     /locus_tag="IYE22_002020"
     CDS             complement(8874..10145)
                     /locus_tag="IYE22_002020"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ9507225.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
BASE COUNT         2873 a         2163 c         1774 g         3435 t
ORIGIN      
        1 aaacgctgat ttcacaacct ctttgttcat caaaagataa tattgtcttc taacaagtcc
       61 atattcaaaa taacgatatg atggtcttta acggtgataa taccatcttt tttcagttgt
      121 tttaaaattc tgtttacact gctagcagtt gagatgccac aaaatttggc gatttcttca
      181 ttcgtgatcg aaaaatcgat cagcatccct tcttctgtcc gcacaccgaa taaatcatat
      241 aattcgtaaa gttgcgtaca aacagctccc agctttccgt ttaatagcat ttgctgcatt
      301 tttttcatcg aataaagcag acgatagcgg taatactctt ttacatagtg ttgtagcttt
      361 gggtcttggt tgatattttg ccaaaagcgc acacgattga ttcgataaaa ctctgcatat
      421 tctgattcaa ctcgaatatt atacggcgaa tcgatgaatt gtgaatattc gtcttttaaa
      481 agagagacga tttcaagatc tttgatatag cgaaggttaa attctctccc atcatttgaa
      541 ataacacttg cttttaccgt tccctctttc aagatatacg tatgggaatc ttccatccct
      601 tcgaatgtca aatatttatg atatcctttg cgtataatcg gaaaatcatt tttatccaaa
      661 tattccttta agactctact atccaatgat gctcccccat ccaaactttc atctaaaaag
      721 atcatacagt actttaaaac tttttaaagt ccaaaggtat tttactaatg atattaacat
      781 ttgttaagta cctttttcca tcacttatgc gatttttttc ctttttttta ttcgttatac
      841 tgcagaagta aaatttttta agaatagagg gaaaaaacat gaattactgg atgaacacaa
      901 tcattaatcg attagaaaca gcatatcaaa caagatttga tatgaaagct tctttagtct
      961 ttttaaatga tgcgtatcaa aactcgatcg aattgatcaa agcagtggat gagaatccaa
     1021 caaacgaatg cgaagaattt ttaaatctgt ttatgtctac aagagattta tttattcgcc
     1081 aacttgttga tcgctatcca tccaattatc acgatgtaga agttcaaata caaaaattaa
     1141 aagcatattc agcttaaggg gaatgtataa catacatgga aaagaaatta tttaataaat
     1201 attttattgg aatcacattg atcaatttta ttgtctacct gatttattat ctgttgatgg
     1261 tcatcatggc agtcattgcg caagacacct tacacgccag cttgagccaa gcaggttttg
     1321 cttctgggat ctatatcatc ggaacattgc tggcacgctt attcatgggg aaacagttag
     1381 aactatttgg tcggaagttt accttaagag gcggcgcgat cttctatctc ctttcaacat
     1441 tagcatatct ttatacgcca actatcgggg cattgtattt agttcgattc ttgaatggat
     1501 tcggctacgg tacagtttct acagctacaa atgctatcgt tactgcatat atccctgcat
     1561 ctaaaaaagg ggaaggcatc aattattatg gattaagtac tagtttagct gccgctgttg
     1621 ggccattttt aggaatgatt ttgctgaata tgactgattt tcgtttcatt atctggtttt
     1681 caattgtact ggtcttcttt gttacgattg cctgccttgt ctttcctgta aaaaatattc
     1741 cattatctga agaacaaaaa atatctcttc gctcatggaa tatcaatagt tttattgaaa
     1801 agaaagcact tttcattaca ggaattgcct ttttgatggg tctttcttat tcaagtgttt
     1861 tatcattttt atcttcttat acaaaagtaa tccacttagt tgctgctggg tcatttttct
     1921 tcgtcgtata tgctttggtc atcacattta cacgtccatt gactggaagg attttcgatg
     1981 tcaaaggaga acaatacgtg atgtatccta gctatttatt cttaacagca ggattatttt
     2041 tactgagtgt aacgacgaat agtgtgacat tgcttatctc tggagcatta gtcggcctag
     2101 gttatggtac gtttatgtca aatggacagg ctgtttgttt aaaaatcgtc cgcgaacatc
     2161 gaatcagtat tgctttatct acctacttta ttggtttaga cctaggctta ggcgttggtc
     2221 cttatttgat ggggatgcta aaatctctta cttcatttag aggattgtat gtgattgccg
     2281 gagtcattcc attgatatgt acagcactct atcttgttaa tggccgaaaa catagtcatg
     2341 aagcagaggg cgaattgatt gaatcattga aaaatgaaat ttgatcttga tgaatagata
     2401 aaaaaaaccg ttcagatctg aaaataatct gagcggtttt ttgagtaaaa atagtttatg
     2461 attattctta attaaacatc tatttatttt taaagctttc atttatcgat aagaatagtt
     2521 taactgttga tttattccat tttccgttca tctggtacga tgccttgact tagcaaccac
     2581 ttgtggaaga accattctgc cacaccgatc aatagagcag gaatcaatac tttcatccaa
     2641 atatcgactg tatcattcat aaaatataga tagagccaca agataactgc agctgaacca
     2701 gcatctgcga tcgtcgctgc gatgttccct actttacgta agatcaacag atcccctagc
     2761 acatagatca tgactacaat aattacccca acaatcgttg aatccataaa gctaacaccg
     2821 tataaacctg tcagcacgac ccacagaatc gcccagatca tgattgcctt cactaccaac
     2881 gccttcacat gtttcatttt ctgcactcct ttctcctgga taaaaaagta agattatcta
     2941 acaagataac cttttcatta ttataccatc ggctgagaga agcgagcaaa gataaggtcc
     3001 tatctaaaaa tgaggaagcc acttagaaca ttcacgtaat aatttatccg tacagacatt
     3061 cgatatggtg ttcctttact ttcgcatact gttcttttgt cagttcttta cttgtcacca
     3121 gataatcgat ttcatgaaaa gcaatggctt gatgcatgaa tttatgtgca attttttgct
     3181 gtgtaactaa aacggcaagc cggtctgatt gttttctaaa agcttttttg accattgcct
     3241 ctccttcatg agtttctgtt actccttggc tgaaatctag cccgcgacaa ctcattatcg
     3301 ctaaatccgc ataatatgac aatgcatcgt tataggcttt tgaaccgttg attgttgctc
     3361 gtttcggaac aattgcccct cccaatacag atacttttgc cgttgtatat tcactcaaca
     3421 tagcagctgt gacgatgcca ttcgtaatga tctgcaaacc atcaaatctt gctaggtttc
     3481 tgactaagtg taaagaggtt gtactggaat ccaagaataa gaccatatca tctcgaatca
     3541 aagaagaagc tgtatctgca atcttacgtt tttctgtttc ttcttctaat ttggtgatct
     3601 tcatcaggta aggcacgtca atttttcgat tgtcaataag catggcgcca ccgcgaactc
     3661 tacggatcaa tccacttttt tccagaaaaa caaggtcacg tttaatcgtg gcttcgctat
     3721 aatttactaa ctcgatcaaa tcggtgactc ttgcaaattg gttgtttttt agataattca
     3781 caatctcctg ctgtctattc tccacttcat tcaacataaa atcaccccat ttactttata
     3841 tagtaacagg aaaaaagagg ttgtgacaga agtgattggc ttcgagaaat aagaagaaat
     3901 ttttcgaaaa aactacttct gaacatcgtg tgtggttaag agggtatgac aagactattg
     3961 tcataccctc ttgaccacac atttttattt ttagtataaa ctagccttgc ctactgtgtt
     4021 caaaatttcc attttatgtt tgaccacttc ttgtaccttt ttattagctg ctggataaat
     4081 tgcattcggc tcgtacatcg atggattttc actcaatatt tctctcaaag cattgaaaaa
     4141 gacgctttta agatctgagc tcaaattcac tttggcaact ccgtatttta ctgcttcgct
     4201 gacttctgcg tccggattac ctgaaccgcc atgtaaaaca aatggaagat cgatttgttg
     4261 gttcaattct ttcagtaagt cgatatttaa ttttggagtt ctgtttttcg gatacaagcc
     4321 atgcgctgtt ccaattgcta cagcaagtgt atcgattcct gtttctttca caaaaatctt
     4381 cgcttgatca ggatctgtat aaatgattgt ttcagcacct ccttcagcag aaccattatt
     4441 cccaatcgtt ccaagctcag cctccactga gacatttacc gcatgagcta acgcaaccac
     4501 ttttttcgta atctgtacat tgtcgtcgaa tgatttcaaa gacgcatcga tcatcacgga
     4561 agaatagccg tttcgaatcg cccgtaagac atcttctatc gtaccgccat gatccaagtg
     4621 gataacgatc ggcagtttac ttgtataagc ataggctctt actgccgcaa caaactcatc
     4681 acttaaatag gcgatttcat caggatgaat ttctaagatt actggtgctt ctaatctctc
     4741 tgcttcttcg atgattgctt tcatcatatc atagctgcaa atattgaagg ctggtactgc
     4801 aaattgattc tctttagcca ctttcaataa ctctttcatt gtatataaca ttacgcttcc
     4861 acctcatttg ttagattttc atttttcgtc cacttgtctt gttttttctc tttcactgtt
     4921 tttttcaata gtccaagcat aaaggctgta gtgactgtac ctgatgcaat tgccaagaca
     4981 tacataggta aattgttcat aacatttgga attagcatca cccaaatccc tccatgaggt
     5041 gccattgaag taactcctac tgccatggaa atccctgcag cgactgctga acctgtcatc
     5101 aaactaggta tcacgcggat cgggtcagat accgcaaacg gtatagcacc ttctgtaata
     5161 tacgaagcgc ctaaaatcca gcatgattga cctgaagtcc gttcttcttt ggtaaattta
     5221 tgtttgaata ataaagtggc taaagcaata cctaggggag gtgtcattcc tgcagccata
     5281 caagcagcaa tcggtgcatt tactccagct gacatcaaac cgatcgagaa agtcgaagtc
     5341 gatttattga tcggcccacc catatcagct gccatcatga tcccgataag taatccgaac
     5401 aatagcccgg atgtatttcc caagctattc agccaatttg tcaaagcatc caacacccat
     5461 gtgatcggtt gttcaatcac aaataccatc aaaagcccaa taattaagat cgataaaaag
     5521 gggacgacaa tcagctgata aatagcagaa aattcttttt ttacgctaat tttatttgcc
     5581 agaaaatcac agatataccc tgctagcacg ccaccaatca ttccgccaag aaaacctgaa
     5641 ccacctgtag aggccagtaa tccggcaaca attcctgggg caaaacctgc ttttccagca
     5701 atcgatgtag caatccccgc gcctaacgca ggaaccatca tggcaaacgc tgtatctcct
     5761 ccgatccttg aaaatgcagc tgctaacaca ttatagtctg ctgagttggg atcagccgct
     5821 gtaatcccaa aggcaaaact cagtgcgatg atgattccgc ctgcgatcac aaatggaagc
     5881 atgtaattca caccattcat gaaatgatta tagattgtcg gctttttctc atttgcagct
     5941 ggttcttttt cattagacac aacgaatacg ccaatatctc tcaatgcttc gtctattact
     6001 acttgcggtt ctttgattgc tcggcttgta gagacacgct ttactttttt cccggcaaat
     6061 cggtccaatt cgatttcttt atctactgca agaatgattg catctgcctc tttgatttct
     6121 tgttcagata actgattttc tgtttttaca ccttgtgttt cgaattttgc ttgatggttt
     6181 agatttcttg ctgttacttc taatttttct gccgacatat aagtatgagc aattccagtc
     6241 gggcaagcag ttacaccaac gattttcatg attattttcc tcctttttct tcactatcga
     6301 tactcggaca aagactctac tattttcatt cttgactaat ctgtctgatg atatctgctg
     6361 gcgtagtggc ttcttttatt ccctttctaa aatctgcatt catcaatcgt ctggagagtg
     6421 tgctgagaat ctctagatgc atatctgctg atttttcagg aactacgatg gcaaaaatat
     6481 attcaattgg catttcatct atcccgctat agtccgttaa tttggctgta tgaaaaaaac
     6541 acagtgctgg ttttgtcaca tatttgcttt ttgcatgggg aatcccaaat ccatcttcta
     6601 ttcctgtgga gatctcctcc tctcgtttga atagtgcctt aacgactttt tgaacatttc
     6661 ttaccttatt tgtttttttc aacacatccc ctattcgctc aaataatgat ccttttgaat
     6721 ctatcgatgg atcgaaaaca atcagttctt ctgcgatcaa ttgatcaaac ctcataaaaa
     6781 acctctcccc cacaaaaaat gttaacgttt tcttggaact gaggtcagta tagcatcgct
     6841 agctgtaccg ttcaatatta tgaatgaaca gaggaaaaag acccatcatc cgcgtgtata
     6901 agtcattaaa atgaacgatc tagctcacaa acaatcgcaa gtctataaca aaacaaaaag
     6961 ccttccttga aaggaaagct tagcttctaa ttttaggtaa atggtttttt acttcgaaat
     7021 aacccctctt catttcgtta atataaggtg tttttatcaa gtggataatt aaaactatat
     7081 actagttttt cattgttttg tgcattatga tcctacgact ttttcagcat caaacgaagt
     7141 tttaaattgg caaaaaacat aaaaaaacag actatcccta tcagatagtc tgctaaacgt
     7201 tgagttatca gcgtttttat tattccgcag cgtttgcgtc tttgagaccg tattttttgt
     7261 tgaaacggtc cacacgtccg tctgcttgtg tgaatttttg acgtccagtg tagaatggat
     7321 gtgagtcaga agttacttcg acacggatga ctgggtatgt attaccgtct tcccattcaa
     7381 ctgtttcttg tgaagttttt gttgaacctg acaagaattt gaaaccagtt gttgaatcca
     7441 tgaatactac tgggtggtaa tctggatgga tgttttcttt catgattttt tcgctccttt
     7501 gccctgctcc atttgctgaa ccagagttga tttgtcgatt tacaacatta acatcttaac
     7561 atgtttcagt caaggttgca attatctttt cagatttctg gtcggatttt ttttaccaaa
     7621 ggagacatct tgaaattctt tgaaaaattg ctgattgttt tttgttttac gcaaaaagcg
     7681 gatcagttgt tccgtatatt ctaatgaatc tccagtcata ttttttcgaa gtttccaaat
     7741 ttcttctaat tcttcacttg gcatcaataa ttcttctttt cgggtacctg attttttgat
     7801 atcgatggct gggaagatcc gacgttcaga taattcacgt gaaagttgta attctagatt
     7861 tccagttcct ttgaattctt catagatcac gtcatccata cggcttcctg tatcgactaa
     7921 agcggtcgct aaaatcgtca aactgccacc ttcttcgatg tttcttgctg ccccgaaaaa
     7981 tttcttaggt ttatacaatg cggctgggtc aatcccgcca ctcaatgtac gtccgcttgg
     8041 tggaacgact aagttatagg cccgagcaag tctagtaata ctatccatca agataacgac
     8101 atctcgttta tcttcgacta aacgcattgc gcgttccaat actaattcag aaactcgagt
     8161 atggttttgc ggctgcagat caaatgtaga agataccacg tcgcctttca cgcttcgttc
     8221 tagatccgtt acttcttccg gtcgttcatc aatcagtaga acgattaatt ctacttccgg
     8281 atagttttcg gtgataccat ttgcgatttc ttttaataca gaagttttcc ctgcttttgg
     8341 cggtgcgacg atcaatccac gctgaccaaa accgattgga gaaaagatat cgatcattcg
     8401 ggtagatagt ctttgcggtg ttgtttccag ttgaagctgt ctttgcggat aaagaggtgt
     8461 taaggctgga aaatgcggac gttcttttgc ttcttccgga tcttttccat taacgctttc
     8521 cacgtgcatc aaaccataat aacgttccga ttctttaggt ggacgggctt ttcctgccac
     8581 tttgtcccca tttctcaaac tgaatcgtct gatttgtgag gcagaaatat agatatcttc
     8641 tgcacttggt ccataattga ttggccgtaa aaaaccgtaa ccatcttgac caacgatatc
     8701 caatattcct tccatataat aaaatccttg tttctctgct tgtgcacgaa tcacagctag
     8761 agataattcc tttttattca tcttactgta ataaggaatt ttaaattctt tggcataact
     8821 ataaacttct tttaacgttt tgttctctag ttctgccatt gtcaaataat cgctcattca
     8881 gctcccactt cctctatcat ttcaattgat gcccctaagg ctgttagttt ctcgataata
     8941 tgatcataac cgcgtaagat ataatctaca ttgtaaatcg tcgttgtccc ttctgccaat
     9001 agtccagcaa tcactaaaca agcacctgcc cgaagatcag aagccacgac ttctgtgccg
     9061 tgaagtttat ttggaccatt cacgatgatc atatttcctt ccacggtaat atcagcgccc
     9121 attcgtgcta gttccggcac atggttcaca cgtttcgcat agatcgtatc gatgatttct
     9181 gaagtgcctg tagtcatcaa tagtaacgca gtaagtggct gctgcagatc tgtggcaaat
     9241 cctggataag gataggtcat gatgtttgcg ggttttaatt ctttcgaggg gtaaacttcg
     9301 atttcatctt cgctgatttt catatggaca ccaatttcct gcaattttgc tataaagctt
     9361 tccaaatgtt caaagatcac atttttcact ttgataccat ttcctactgc agctgctaat
     9421 gctagatagg ttcctgcttc gatgcggtca ggaatcatga aatgccgaca gccatgtaat
     9481 gtttcgactc cttcgatacg gatcacatct gtacccgctc ctcgaacttt tgcccccata
     9541 ttattcagta aagttgccac atctatgatt tccggttctc gtgcagcgtt ttcaattact
     9601 gtctgtcctt ttgcttttac agcagctaac atcacattga tcgttgctcc gactgaaacc
     9661 acgtccatga aaatccgatt gccacgcaat cctttatttt ctgtccgtaa atacatggca
     9721 ccatgttcat ttgtcacttc agcacctaga gcttcaaatc ctttgacgtg taggtcaatc
     9781 ggtctagggc ctaaataaca gccacctggc aggcctacta ccgcttcgcc aaatttacca
     9841 agcagtgttc ccataaaata ataagaagca cgcaaactat tgatttttcc gtgcggcatc
     9901 ggaatcgata cgacttctcg aggatcgatt tctagtatgt tgttggaaaa atggacagtt
     9961 gcgcccatga tctctaaaat ttcaattaat gaatgaacat cttgtatgtc cggcacacct
    10021 tccaagatca ctggggaatc agctaagatt gcagctggaa tcaatgcgac tgcactattt
    10081 tttgcaccgc tgatcgttac ttcaccagct aaaggtcggt ttcctttgat tacgattttt
    10141 ttcatctgtt tccaccttgt ttattaatct tttttcatgg atatgcttgt attttatcat
    10201 agataaaaaa aagaaacgag catggtctgc cgttttaagt aaaaa
//
LOCUS       DACIRY010000095        10164 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.097, whole genome shotgun sequence.
ACCESSION   DACIRY010000095 DACIRY010000000
VERSION     DACIRY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.097"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            1..369
                     /locus_tag="IYE22_002021"
                     /pseudo
     CDS             1..369
                     /locus_tag="IYE22_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(495..1574)
                     /locus_tag="IYE22_002022"
     CDS             complement(495..1574)
                     /locus_tag="IYE22_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9507226.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1677..2117)
                     /locus_tag="IYE22_002023"
     CDS             complement(1677..2117)
                     /locus_tag="IYE22_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9507227.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2174..2584)
                     /locus_tag="IYE22_002024"
     CDS             complement(2174..2584)
                     /locus_tag="IYE22_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ9507228.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2764..3198)
                     /locus_tag="IYE22_002025"
     CDS             complement(2764..3198)
                     /locus_tag="IYE22_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ9507229.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3298..4146)
                     /locus_tag="IYE22_002026"
     CDS             complement(3298..4146)
                     /locus_tag="IYE22_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9507230.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4535..6301
                     /locus_tag="IYE22_002027"
     CDS             4535..6301
                     /locus_tag="IYE22_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ9507231.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6403..7662
                     /locus_tag="IYE22_002028"
     CDS             6403..7662
                     /locus_tag="IYE22_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ9507232.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7809..9110
                     /locus_tag="IYE22_002029"
     CDS             7809..9110
                     /locus_tag="IYE22_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9507233.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9113..9964
                     /locus_tag="IYE22_002030"
     CDS             9113..9964
                     /locus_tag="IYE22_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9507234.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9977..>10164
                     /locus_tag="IYE22_002031"
     CDS             9977..>10164
                     /locus_tag="IYE22_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ9507235.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLS"
BASE COUNT         3424 a         1824 c         1894 g         3022 t
ORIGIN      
        1 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
       61 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
      121 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
      181 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
      241 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
      301 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
      361 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
      421 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
      481 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
      541 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
      601 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
      661 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
      721 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
      781 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
      841 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
      901 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
      961 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
     1021 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
     1081 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
     1141 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
     1201 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
     1261 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
     1321 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
     1381 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
     1441 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
     1501 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
     1561 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
     1621 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
     1681 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
     1741 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
     1801 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
     1861 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
     1921 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
     1981 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
     2041 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
     2101 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
     2161 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
     2221 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
     2281 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
     2341 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
     2401 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
     2461 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
     2521 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
     2581 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
     2641 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
     2701 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
     2761 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
     2821 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     2881 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     2941 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     3001 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
     3061 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     3121 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
     3181 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     3241 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     3301 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     3361 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     3421 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     3481 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     3541 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     3601 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
     3661 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     3721 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     3781 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     3841 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
     3901 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     3961 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
     4021 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     4081 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     4141 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
     4201 tcaccttaag ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca
     4261 ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg
     4321 gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa
     4381 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
     4441 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt
     4501 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
     4561 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
     4621 taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac
     4681 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
     4741 ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt
     4801 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
     4861 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
     4921 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
     4981 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
     5041 ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac
     5101 ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
     5161 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
     5221 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
     5281 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
     5341 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     5401 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     5461 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     5521 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     5581 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     5641 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     5701 tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     5761 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     5821 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     5881 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     5941 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     6001 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     6061 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg
     6121 tgaagataag ctctatttca cacggaaact agagggacag atcatctatg tttactttaa
     6181 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     6241 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     6301 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     6361 taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg
     6421 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     6481 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     6541 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     6601 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     6661 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     6721 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     6781 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     6841 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     6901 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     6961 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     7021 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     7081 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     7141 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     7201 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     7261 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     7321 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     7381 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     7441 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     7501 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     7561 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     7621 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     7681 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     7741 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
     7801 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
     7861 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
     7921 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
     7981 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
     8041 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
     8101 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
     8161 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
     8221 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
     8281 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
     8341 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
     8401 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
     8461 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
     8521 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
     8581 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
     8641 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
     8701 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     8761 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     8821 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     8881 atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg
     8941 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     9001 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     9061 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     9121 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     9181 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     9241 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     9301 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     9361 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     9421 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     9481 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     9541 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     9601 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     9661 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     9721 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     9781 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     9841 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     9901 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     9961 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
    10021 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
    10081 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
    10141 gattccatta gaacaatttc tatc
//
LOCUS       DACIRY010000096        10150 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.098, whole genome shotgun sequence.
ACCESSION   DACIRY010000096 DACIRY010000000
VERSION     DACIRY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.098"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(650..1630)
                     /locus_tag="IYE22_002032"
     CDS             complement(650..1630)
                     /locus_tag="IYE22_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ9507236.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1643..2521)
                     /locus_tag="IYE22_002033"
     CDS             complement(1643..2521)
                     /locus_tag="IYE22_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ9507237.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2537..3337)
                     /locus_tag="IYE22_002034"
     CDS             complement(2537..3337)
                     /locus_tag="IYE22_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAQ9507238.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            4729..5055
                     /locus_tag="IYE22_002035"
     CDS             4729..5055
                     /locus_tag="IYE22_002035"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ9507239.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            5063..5971
                     /locus_tag="IYE22_002036"
     CDS             5063..5971
                     /locus_tag="IYE22_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ9507240.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6600..6851
                     /locus_tag="IYE22_002037"
     CDS             6600..6851
                     /locus_tag="IYE22_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ9507241.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            7393..7551
                     /locus_tag="IYE22_002038"
     CDS             7393..7551
                     /locus_tag="IYE22_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9507242.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            7623..8849
                     /locus_tag="IYE22_002039"
     CDS             7623..8849
                     /locus_tag="IYE22_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9507243.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(8938..9330)
                     /gene="rpsI"
                     /locus_tag="IYE22_002040"
     CDS             complement(8938..9330)
                     /gene="rpsI"
                     /locus_tag="IYE22_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ9507244.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(9346..9789)
                     /gene="rplM"
                     /locus_tag="IYE22_002041"
     CDS             complement(9346..9789)
                     /gene="rplM"
                     /locus_tag="IYE22_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ9507245.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         3160 a         1959 c         1727 g         3304 t
ORIGIN      
        1 actgtctaaa aaatatgcgg cgcacagaac gcgccgcata tcggactaag ttttattcaa
       61 atttgtcttc gccaacacaa tttgacgaag agccttttct ttcaggaaga cttcggtaat
      121 ttttaggagt tctacctatt cttttaaccg tttattctct ttttctaagc gtgtaatttc
      181 atttctgcga atggatttca tgacttcctc taccgagaaa agtacgttat ccatattttt
      241 caacttcttg aactcatcaa tacacgctat tagagcgagc ttctagctat agactaactt
      301 ctttaacaga aaacccttct tccgttacca gttttacagc ggtaatttta aatatttcat
      361 caaatgaacg tcttggcata atccagatct tcgctttctc ttaatttggt gtcaatttta
      421 gtataacccg tccaacttat tttttaacta agtgcccggt ttttcttgac aactccaccc
      481 aaaagttaga ttttttggtc taagttttag ggtcgcttca ttactagaat tcaacaagag
      541 atgtttttta ttttatttaa attatggggt cagtttccat ttgattacaa acttctttgt
      601 tttctttttt taaacgcaat aataaaggtt ttaatttcag cctttttaat tagaaaatca
      661 attttcccaa attattttct ttagctttct taacgatttc tgtcgcaact accacatcta
      721 atacagccga accaactgtt ttgaagatcg tgatttgttc tccacttgtc cgaccgatca
      781 attcttcatt gatcagttgg cctagttccc catgatagct gtctctttga atatacccat
      841 cttgcagcgg tgaaatgaag tccccagctt cttttagaac accgtccatg gtatcaaaaa
      901 taacaacatc tgcggctttg attatttctc gtggaatttc acacatttcc ggtgtgtaag
      961 caccgactcc attgatgtgc gcaccttttt tgacccactc agcagagaat gtcgcacgtt
     1021 ttgaagtggt cacacttgta atgatgtctg ctccttcaac gcattcttga ctggtttttg
     1081 ttgggcgcat cgttacagaa aattgttgca tcattttttc agcaaattgg ctggcacgct
     1141 caaaatcaat atcaaaaatc cgaacttctt ctaatttacg aacggtcaac attgcttcta
     1201 attgtgactg tgcttgtccg ccggttccaa tcagcgcgcc aattttagca tcttctcttg
     1261 caagaagttc tgttgctgct ccttgaacag cgcctgttct taattgtgtc aaataagtac
     1321 catccaaaca agcggatacc atacccgttt ctggatccaa tacgatcatc gttgctggta
     1381 cacttggtaa gtttttcttg atgttttcag gatacacgga aacgattttg acacctagcg
     1441 cgccttcgcc accttctaca taagcaggca tatataagct ttgtccgttg ctttttggca
     1501 catcgatatt ggtgcgaaga ggcacacttg cctttcctgc tgaatacaat gacagtgctt
     1561 ttttgtccgc atcgatggct tctctcattg aaaaactttt gataatatct tcttttttaa
     1621 gaaataacat ttgaatcgct ccttaattag ctgctgtaac tttttttaat tgactgccca
     1681 acatcgtata tgcactgtat agtaagacca aaatcacgat ccatttcagg atataggtat
     1741 ctaaagattt gactaggaat acagcgacca atacccccaa tacaccgaag gttgaagtaa
     1801 aaagtgtgat ttttctgctg tattcattaa atttaatgaa ttgaacactg ccgatcggta
     1861 ctgagaaggt acaagccccc atcataatag gaaaggctgc aactggattc aagcctagca
     1921 agtagacagt taccatcgtt aacgcataag aaccaatgcc gatattgttc aatgcaccat
     1981 agacaaagag taaaaatcct gccaagatta attttccgcc atacaattca ctggctgttc
     2041 cgtttgatgg aatccagttc atttgtcctg caaaaatcaa caatgcagca atgaccaatc
     2101 ctatgccgac aaacagtttg atcgttttct ctggtaactt cacgacaaat cttggtccta
     2161 aatatgcacc gataatttgg cagataatac aaacagctag tgtcttgatc cctacgttga
     2221 tggcagtgat gtaagacaat gccatgacgg ctaccggtac tacacattgg gtattcaacg
     2281 ttccgggtag ttttttcatg ctgacccatt tcaactttgg ataaaggacg gttccgatag
     2341 caaagtcgga aacgccaaat gttgaaagaa agaagatgat aaatgacgtg aagggcaaca
     2401 gacgcatatc ccctggttct tctttgaaat tttgtttgtt tttgaaaagg tctcttagaa
     2461 agtaaataat aaaatataga ttgattgcga caattagtgt cagcaaaata ttccgaatca
     2521 taaaaaattc ctcctactag taaatctttc tgattccctc aaagccctca ccatattttg
     2581 ctttaagttc ttcaggagat tctgcaatca cttttagtgt atatgcatca tgtttgacat
     2641 attcttttgc tagattgacc atggcgtggt tttttgtcca agtgacatcg gttgattgca
     2701 gttcttcgtt aatggtacca aacacgactt cttgctcatc ggcgacgatg ttgatccatc
     2761 ttgctccaaa gtcagcaagt ttgtctgttt caaaaccatg aatataatac ttttctaagg
     2821 gaagatcata ttcatgggta gaactgaata aaatcagtac aaacttatca actctttttt
     2881 cagcttgctg caatgccttt agtagtgaat ccgtgaaatt ctcatgccaa acttgaatga
     2941 gcaaactttc ttttgcgttt ttcaccaaat gctctacttt atccagaaca tactcgatac
     3001 catcaacttt ccacagcatt tcttcttcat ctttttcttt gatcatccct agattcttgg
     3061 taagaaaaga aagatcattt ttaacacttt tttctaattt ctctaaaaac tcatttgccg
     3121 agatggcatg ataaagttta ggttctgatt tattaacgac aatcaaattc ttttttaata
     3181 gtttttccaa aatattatat actttcgagc gtggcacacc tgagatttta cttacttcat
     3241 atcctgtaag attacctttt tctaatagtg tggtatacac tcttgtctcc atctctgaaa
     3301 aatcatattt tttcatgatt tgaataatcg tttccattga ttaatcctca ctttattttt
     3361 agtagctact acggtaacaa ctataaattc tttttgatta tctgtcaaga gttagttcaa
     3421 taacacacat ataataatta gcttaaaaac aatcttttta ttaaaaagaa acattataat
     3481 atgaatgttt gtgaaaaaat aatacaactt agtcattctt tacaacgatt tatagaaaaa
     3541 caacctcttt aaatataaaa ggtacgggtt actgattact tgtaaatctt tacgttccag
     3601 ttttattctt tctaaaaaat atactcactt cttttcttag acttcttttt ttgattctcc
     3661 tctttatggt cttgtccttt tttgccttca aaatatttaa ataaacccct ttcaaaagca
     3721 tttctaggtt ttggacaata aggaatgact tttttcttca tataaatacc tcatttaact
     3781 atttgatatc atcacattat caaaaaaaat aggtttttta ttaaaactat cttgaccttt
     3841 cgggagattt cactgtgatt tatttttatt cccatacttt tttcgttggt cactttcgta
     3901 tacctaatct aagcgatcat cggcgacggt gcttttacaa cgaatggcag gacaaaacaa
     3961 ggggcggaaa aacttttttg cctatttcat aaaaaacttt ttcctacagc aatcccttgt
     4021 tttgtcctac cacacgttgt gaacccaaag cacgccgacg aacgcttagc tatacgaaaa
     4081 tgacctccaa aaacaaaaaa gtgaggtgaa tagaaacaaa aagaaattga aatctcccaa
     4141 aatgtcgtcc gcctcggaga caacttgctg ctgaaaatgg ttggttaatt catttaagta
     4201 ttaaataaga taaatcatca aattaatcgg aaaaatagca tgattggcct tatcaaaaat
     4261 aaactgtaaa aacttatatt tctatgagtg gtggaacaat gctaatacca aattaattta
     4321 tcgattaccc acctgcacaa accaaagata gcattattag gaaacgaatc aatcagcgaa
     4381 tccagcatta taatgaggat actaaaaata aagttttcat aattataaat ccgccttcct
     4441 acgaaaggca ccaatgaggt gatgaaaacc ttagagcgct ttctttcttt ttttattccg
     4501 tgttaccctt tccttgcagg tccctggtac actacagaac ccgaggaggt gagtggcggg
     4561 gctgtatagc ggcaaccccg cataattata tggtgtcggt catccgttaa ggcatcaatg
     4621 aatggcgcaa acccgtagcc cgtaaactta tctcttccga agttgactcg atttcgccgg
     4681 atgaccagtc cctaccaaac ctgtactatt tttttacagg aggttgctat gttcaaaatc
     4741 tttcgtaaaa attgttgtgg cttggatgtc cacaaaacgt ggatctatgc gtgtatcgga
     4801 atcacagatg cgaatggtcg tacagtgtat aagcaagctc gcttctcttc ctttacaaaa
     4861 ggtctcaatg aattagctga atggcttgct aaatacaact gtaaggatgt gtgtttggaa
     4921 tcttccggta aattttggat tcctgttttt aatattcttg aaaaaacttg ttttgtcaca
     4981 ctggctcatc cgaagtacac caaaccccag aaaggaaata aaacagatcg caaagatgca
     5041 aaaatggatt tgtgatctct ttatgtgcga catgatcaaa cccagtttta ttcctggtct
     5101 tgaaatccgc cagcttcgtg atctaatgcg ttatcggacg aaactgacca acatgaaaac
     5161 aagtgaaaag aatcgtgctt taaactgctt aaccgtttct aatctgaaac tagatgatgt
     5221 ttttagtgat gtgttcggaa aatcatccac ttctatcatc caatacttgt tagaacatcc
     5281 cggtgaatca tttgatgttc gaccatttat ccatgcccgt tgtaaaactc ccgttgaaga
     5341 aatccaagcg gcagttgagg gggcgatttc tgtacctcaa gcgattaagc ttcgccaatg
     5401 cctcaaccat attaacgaga ttgagaagca tcgagaagag atcaatctag agattcaagc
     5461 tcttgcagaa cccttctccc ctgttttgga actacttcgt accataccgg gcttagacac
     5521 tcagccaata acagcgattg cgattctttc agaaatagga ccggatatgt ccgtgtttcc
     5581 aacttcgaaa cactttattt cttgggctgg atgctgtcca agaaacgatc gcagtgcccg
     5641 acgaacaaaa tcaacacgta tctctcgtgc agggcagtcg ttgaaaccgc tacttgtaca
     5701 gattgccaat gctcttctca gatccaaaga acatccagaa tttaaaaatc gctatcgtag
     5761 aatcaaagcg aatcgtggac acaagaaagc tatcattgct atttgtaaga tgctcctgac
     5821 cgctatctgg aatgtcttat caaaggtgga accctattct gctaaaggat atctggagcc
     5881 aaaacctgcg aaagaagaga aagttctgac gcaatcccaa gctctggacc ttttaagaaa
     5941 acgcggatat accattatag atagtgctta aaccaaagtt acgttgattc ttcctaaata
     6001 gactcgttgt cttccatttc aaaaattttg aaaacggtag gcttatttag tgtaccgttc
     6061 ttcaggtatc ttcaaataga tttattttca aacttatttc ctttcttctt ttactctcct
     6121 atagcttagc atttaagtgg gcactcgtgt caccttgcta gatagctatg aatgccgtaa
     6181 taagcctcct agtatatagg cagactgcca attataactg attcaatcca cattatgata
     6241 aaatccacct tttcgtttca tatgtgttga aatatcacat atgtcaaaag tgcacgagtc
     6301 ttttcattca gtgctttgta aatactcaaa attattcctt ttcactttaa aaaaaagata
     6361 attagtacaa aatcagaaat gaggaaagta ttaggatgga ctagaaagga gttgttgaat
     6421 ttgttgcaac agattggtca tttatcaatt aaaaattagg tgaagtttta ttcatcactg
     6481 cagctgctgt ttcccagaga ataaaacgtc tagaagaaac tggtcagtta ctgtattatc
     6541 aagcagcaat tgactacgaa caagcttgat tactaaccaa agcgtttatc cacctgttga
     6601 ttaaatcaaa tcaaaaatca ggtttttata cgttcataaa agcaatctcc agtgtactcg
     6661 aatgtgactg tatcataggt ccttattcga tgttaatcaa aatggggttt ccttctaccg
     6721 aatcattgga cagttttatc aatgatttat caaagtttgg acgaaccaac acccaaatcg
     6781 ttttctcgac tcttgtttcc ttaatggttt atcagttctg gaatgtttat catgcgcttt
     6841 tattaaaata gaaaagagct tttttcatgc gacaaagcag tgatcattta ttggcacatg
     6901 caatctttgt ggatccattt tttgaaaata cgatatttag ttttgaccca aaaatcttta
     6961 aatacgtatt tgataattta tcttaacaac cagataattt tattcagcac cgtattatct
     7021 atattgaatt ctcggaatca agataaattt ttttccgttt ttgtttcttt tttaatcttc
     7081 ttcttgttat taatagtgat taggatgata ttcctatatt gatctttgat aattagatat
     7141 aatttttgaa taacgttccc tgctattcga gctagaaata caagtacgcc atgacctagt
     7201 tagcgacaac tacttagaga tttcttttgt tctgggacaa caaaatgcca tgacaatagc
     7261 agtaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg
     7321 tgcttaccca aagcggttca aaaattttaa actattattt taggagatga ttttataaaa
     7381 tcgaaagcct acttgaataa aaaacaagcc tgtgaatttc taggaattga taataatact
     7441 ttagataagt ggattcgtgt atataatttt ccagtaataa aaattgatgg cgtagttcgc
     7501 tttgacgtag aggatatcaa agatttcatg aatacattca aaaaaaattg aaattttctt
     7561 ctcgtcacat atactataaa tagcgaaatg tactgcctta ctttttaaag gagtgaggaa
     7621 gtttgcctac aaagctttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat
     7681 tcagagaaat cactggaatt gcatcagaga agtatcaaaa agtatttcca actaggcaat
     7741 tagctattaa ggccgaaagg gaaatgaaaa agaagattga aacagtttta agagaggaga
     7801 atgcaaactc actagaattg aaaggaaaaa taaaatttaa agatttttat gaatcaaaat
     7861 ggcttcctag atatgaacta ggtcaaacta ctaggtcaaa cagggctcca agtaatgtaa
     7921 caattagtaa tactaaggat atattccgat tgcacatcct tccaatgttt ggtgaatatg
     7981 caatgaatta tctgaattca aatactgaga taatatcaga tgaattaaca aagaaatcga
     8041 aagaatacgc aaatataaaa attatcaaag gctatgttcg ctctatgttt gatattgccg
     8101 aaatactatg ttatatcgaa ttcaaccgaa ctaccaaaat tattcaatgt attaccgcgc
     8161 ctaagaaaat ggctttagaa gaaaaaagga taagggaagg gaatcaagca ttatcatcag
     8221 aagagctgac tgattggata gaagcagtaa acaatgactt aaataatcac ttgcttacac
     8281 ttcacgatta cacacttttc atgcttacct tatatctagg tgatagaaaa agtgagactt
     8341 atgctctgca atggaaatat attgatttta ataaaaatac ggttcgtctc aaatatgctt
     8401 tagataaata tcaaagaaag acacatacaa aaggttggaa agacaccgtt attcaggcac
     8461 cagaagttgt gatgacatta ttaagagaat ggaaatcagc tcaggctgaa caattgttga
     8521 aactaaaaat tatacaaaca cctgaccagt acctctttac ctattccaaa ccttctggag
     8581 aagtaaattg tcctgttcac gctgactact taaactatcg aatcaatgca ataaaaaaaa
     8641 ggcatcctga attggctcaa ttatctccac acaaacttcg tcatacctat gcaactatag
     8701 ctagggaagg tggagcaaat atgaatcaaa tctctaatgc tttaacgcac tcggatattt
     8761 ctactacaaa aatatacgtg aacacacctg acgtagttga taaatctgtt tttgaagcat
     8821 ttcaaaaagg attgaataag tgtgattaac atgtgttctc aaatgtgttc ttattataaa
     8881 aaaagacact ttccaaataa ttgaaaagtg tcctgaaata ttgatataaa aagaatatta
     8941 acgttttgag aattgtgaag ctttacgagc ttttttaaga cctggttttt tacgttcgac
     9001 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     9061 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     9121 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     9181 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     9241 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     9301 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     9361 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     9421 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     9481 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     9541 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     9601 cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt
     9661 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     9721 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     9781 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     9841 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     9901 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     9961 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
    10021 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
    10081 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
    10141 acggcaggct
//
LOCUS       DACIRY010000097        10025 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.099, whole genome shotgun sequence.
ACCESSION   DACIRY010000097 DACIRY010000000
VERSION     DACIRY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.099"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(574..1920)
                     /gene="gor"
                     /locus_tag="IYE22_002042"
     CDS             complement(574..1920)
                     /gene="gor"
                     /locus_tag="IYE22_002042"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ9507246.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2054..3295)
                     /locus_tag="IYE22_002043"
     CDS             complement(2054..3295)
                     /locus_tag="IYE22_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ9507247.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3313..4236)
                     /locus_tag="IYE22_002044"
     CDS             complement(3313..4236)
                     /locus_tag="IYE22_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ9507248.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4457..6949)
                     /locus_tag="IYE22_002045"
     CDS             complement(4457..6949)
                     /locus_tag="IYE22_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ9507249.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6956..7423)
                     /locus_tag="IYE22_002046"
     CDS             complement(6956..7423)
                     /locus_tag="IYE22_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ9507250.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7559..7632)
                     /locus_tag="IYE22_002047"
     tRNA            complement(7559..7632)
                     /locus_tag="IYE22_002047"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7596..7598),aa:Arg,seq:acg)
     gene            complement(7643..7728)
                     /locus_tag="IYE22_002048"
     tRNA            complement(7643..7728)
                     /locus_tag="IYE22_002048"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7692..7694),aa:Leu,seq:taa)
     gene            complement(7745..7816)
                     /locus_tag="IYE22_002049"
     tRNA            complement(7745..7816)
                     /locus_tag="IYE22_002049"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7782..7784),aa:Gly,seq:gcc)
     gene            complement(7828..7900)
                     /locus_tag="IYE22_002050"
     tRNA            complement(7828..7900)
                     /locus_tag="IYE22_002050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7865..7867),aa:Thr,seq:tgt)
     gene            complement(7918..7999)
                     /locus_tag="IYE22_002051"
     tRNA            complement(7918..7999)
                     /locus_tag="IYE22_002051"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7963..7965),aa:Leu,seq:tag)
     gene            complement(8007..8079)
                     /locus_tag="IYE22_002052"
     tRNA            complement(8007..8079)
                     /locus_tag="IYE22_002052"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8044..8046),aa:Lys,seq:ttt)
     gene            complement(8083..8155)
                     /locus_tag="IYE22_002053"
     tRNA            complement(8083..8155)
                     /locus_tag="IYE22_002053"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8120..8122),aa:Val,seq:tac)
     gene            complement(8169..8284)
                     /gene="rrf"
                     /locus_tag="IYE22_002054"
     rRNA            complement(8169..8284)
                     /gene="rrf"
                     /locus_tag="IYE22_002054"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(8378..>10025)
                     /locus_tag="IYE22_002055"
     rRNA            complement(8378..>10025)
                     /locus_tag="IYE22_002055"
                     /product="23S ribosomal RNA"
BASE COUNT         2634 a         2375 c         1751 g         3265 t
ORIGIN      
        1 acaccgttta tcagaggttg taaaatcagt gttttgtcct gagtattaga gaaacaaatg
       61 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga
      121 acactatttg ttcggaaaaa gtgactaact atgacacaat ttttttcaca gtcccttttt
      181 tcacttgatt tgattcgtac cagcgaactt gacgagaact ttcttggttt ggtaactata
      241 tggtattctt ttaacatcct cgtttgcttc ttctaattta tttatacaaa gtcacacttt
      301 tttaaacatc tgttcttcga tacatttcta tttcttttaa acatcgatac caatattttg
      361 aacttaagag tgcataccaa atggattcat ttactcttcg tcagttacga agataatcaa
      421 aatctccaac ttccctactt atttcgtttt gagaattatc gaagcaatct tacataaata
      481 actaaaaaac aaaaaaccag aaaatcagta tactgatttt ctggttttac atggtttgtt
      541 caataaaaag ctgttggaac tgttaatatt aaattatcgc atcgttacga attcttccgc
      601 tcctgttgga tggatggcta ctgtattgtc aaagtcttct ttagttgcac ccatcttgat
      661 agccacggca aaaccttgca gcatctcatc gacaccaaca ccaatagcat gtaagccgac
      721 gattctttct tcttttccga cacagatcaa tttcatttcg catttttggc gataatcatt
      781 taatgcaaag tacattgggg taaagcgtga acgataaact ttcaattcat tttcgccata
      841 tttttccaat gcttcttctt ctgtcaaacc aattgttgca attggtggat gcgtaaagac
      901 gactgtcgga acaagtttat agtcaagata agaatctttc tttccattaa ataaacgttc
      961 tgataaacgt ctaccagctg cgattgcaac aggagtcaga tctaatttac cgatcacatc
     1021 accaacagca aaaatatgtt catccgttgt attttggtat ttatctactt tgataaaccc
     1081 ttttttgtcc aattccacat ttgtattttc cagacctaat ttttcggtat tcggtacacg
     1141 tccaccggca aaaagtacac aatcaccagt taaggtttca ccattttcaa atagcaccgt
     1201 atattcgtca ttgtttttag taatttcttt agcaatatga tgtgcgtaag tttttattcc
     1261 agcttcttgg tacatttgga tcaaattgtc cgaaagcatt ttatcaaacg ttctaagtgg
     1321 tcgttctttt cgatatgccc acatgacttc actacctaaa ccacgaaata cacctgaaag
     1381 ttcagccgca atgtatccag cacctaaaac gattgctctc ttaggtaact ctgttaattc
     1441 aaaaaatcca tttgaatcaa ttgcgtattc tccaccagga atggtcattt tacttgcacg
     1501 gcctccagta gcgatcaaga ttttaggagc acgataggtt tcaccgttta cttcgatcgt
     1561 ttgactatcg ataaaagtag cgtaaccttt tatagcctcg acatgattgc tatctaatcc
     1621 tctttgataa gcaccgtgta gaaaatcaat atatttttct cgtctttcta ctagaccggc
     1681 gaaatcaaca ttttttaaat ctgcttggat accgtaactt gaggcatctc gttcaattgt
     1741 ttccaaaatc gtacttgctt gccacataac ttttttaggt acacaaccga cgttgacaca
     1801 tgttccgccc aattcattcg cttcaatcag caaaactttt gcgccatgca tccctgctct
     1861 gtttgctgaa gcaataccgc cagagccgcc accaattacg atgtaatcat atatttgcat
     1921 tttgttcctc cttattgaag agatcaatca aaacctttat ttaacgatcc tttttctaca
     1981 gaaaacaagt gtaacacttt tcttgaattt tgtatgcgaa gatgcttaaa acagggatat
     2041 atcagctttt tccttattta cgttttcgga tcatatctcc aggtttcacc ggctgtgcga
     2101 ctggcatagt taaaatcatc attggatttg gtgctcggtc aatggattcg ccttcttcat
     2161 tatgcataac ttcaacggtt tgactgaaat gactgaatcc tggaccataa aattcgattt
     2221 catctcctac agaaaacaag tttctctgac gtatcgtagc aatctgtgtt gcctcatcat
     2281 aagataatac ttcaccgaca aatttatata ctgggatttt acgacgttca ccgaataatt
     2341 gttcgttttc agttggtgta ttgtagtaaa aacctgtagc taattctcgt tgtgctactt
     2401 tccataactc gtcgatccat tcttgcttgc agacatagtt ttcaggatct tccatataac
     2461 tgtctacagc cgctttatag acatttgaca ccgtagatac ataatggatc gatttcattc
     2521 ttccttcgat cttgaagctg tctacgccgt tttcaatcaa ttctggaata tgttcgatca
     2581 ttgacatatc cactgcactc atcgagaatt cttcttccac ttcgcctgta tcagttaaac
     2641 tgtttcgttc ttgaccaaaa ggcatatcga acaatccata cttccagcga caagattgag
     2701 aacatccgcc acggtttgca tcccgcatcg acatatggtt ggacaatgtg catcgaccag
     2761 aataagagat acacatggca ccatggataa atgcttcgat ttcggtatcc gtatttttac
     2821 gaatctctgc cacttcctcc attgaaactt cacgagctaa aacgacacgt tccaaccctt
     2881 cttctttcca gaattccaat gtctcaaaat tagttgctga agcttgagtg gataaatgga
     2941 tcggcagacc tggggcttct gtcgcacaaa tttcgatcaa agctggatca gaaacaatca
     3001 ctgcagaaat accgacatca cgcaaactgc ggaaaaattc ccctgctcca tcttcatttc
     3061 cttcatgggt gaccatatta gcagcaacgt ataccttcgc atcatgttct tttgcaaaag
     3121 caactccttc agccatttct tcataagtaa aattacctgc acggcttctt aaaccatatg
     3181 cattcccgcc aatatatact gcatctgctc cataatggat cgcggttttt aacttttcca
     3241 gtgttcctgc tggcgctagg atttctggtc gtttcaattt tctttttact gtcatttctt
     3301 tcatcatact ccttatttga tagcagaagg atcaatataa aagaatcctg tatctaattc
     3361 acggttttca ggatgcaatt tactgatttc gctagtcatt ttttccgctt tttcttgatc
     3421 ccagctacct tctaaaattt gtttttttgc ttcggcgaat agtcgagtga tgtttacgaa
     3481 tttttcgcct ggagtaaata atccgtccaa tttccatgta cagtaatgat gctgataaag
     3541 ttcattcaat tcattcgaga gattcagatc attgctggca aaaatatgtg tgccatgact
     3601 atcttcataa atagaataat gtgtctcttc ttttttaggt tccgagataa aaagacctct
     3661 ttcacgatct ttttgttcgt cttgtttcgt atagttatag taattctgta gcagtggacg
     3721 tttggattga tggatgcatg tagcaccata cacaagggtt tctacaggaa tatcgagttt
     3781 ttcttccatc gccttcatct cttcaaaagg aacttcacga gcaagtactg ctccaactgc
     3841 gccttttttc gcccagaaat tgatttgacg tgcgctcgtt actaatgttt cagcatcata
     3901 aatatacggt atacgcaatt caggattttt tttcatcgta taaataatac ctgtatcacc
     3961 aactgtgatc acatctacgt ttatttcttt taaaaaagcc aaatattcag gtacattttt
     4021 cattttttca ggatgaatga tcccatttac agcaatattc gcttttttac catacttgtg
     4081 tgctaattct accaattgtc gctgttcttc tcgtgtaaat gaagcaggca gacgcaatcc
     4141 aaattgctct tcaccgaaat atagtgtatc tgcacctgca gcaagcaact gttctgcttg
     4201 ttcaatagtt tctactgtgg ttatgagttc gatcataatt ttctcctcct acaacttcat
     4261 aatagtagca tagctctaaa aactttccaa tacttcttct atttaaagtc ggatttttga
     4321 aaattatttc acaaaaaatc aaaataaagt tcgatatatt ctgtccaaat ataaaaagag
     4381 attgtgacaa aaatcttgtc acaacctctt aatcaagtct tactattata aaaattcagc
     4441 tacacaggcc aagtaactat agggaaccta ctgtttcctt tttaggtccg cgaacattca
     4501 atgtgatctt accttttgaa gcacctactg ttacgtggtc acctaaacga atttctccgc
     4561 caagcaaagc ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga cggattggac
     4621 gtgcaccata ttcaggatca aatccagctt tcccgatcac atcgattgca gatggtgtga
     4681 ttttcacttg aatatcttgt tctttcaatc gtttgataat cgctttgctc atgatttgga
     4741 cgattgtatg gatttcatct ttactcaatg aatggaagac gacagtctca tcgattcgat
     4801 tcaagaattc aggtcggaaa gctttcttta actcttctaa gatacgtttt tgcatggctt
     4861 tgtgatcttt agtaacatct tggacattga atccgacatt tttttcttct cgaatttgtg
     4921 tcgctccgat atttgaagtc ataatcatga ttgtatttct aaagtccact tttctacctt
     4981 tagaatctgt taaatgacca tcatccaata cttgcagaag taaattgaaa acgtctggat
     5041 gtgctttctc cacttcatct aagagaatca ctgaatatgg tttttgacga attttttctg
     5101 tcaactgtcc accttcatca taccctacgt atcctggagg agacccgatt aatcggcttg
     5161 tactgtattt ttccataaat tctgacatat caacacggat cagggcatct tcacttccaa
     5221 acatgacttc tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt ggtccaagga
     5281 acatgaatga accaattgga cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg
     5341 cacgtgaaac tgctttgaca gcctcatctt gaccaacgac acgttcatgc agaagtcctt
     5401 ccagttctaa caagcgttcg ctttcttttt tctccaactg ctgtagaggg acaccagtcc
     5461 actccgatac aactgtagca acatcttctt ctgttacacg atcagaataa ccttgctcct
     5521 gttttgcttc aattgcagaa actttttgca gctttttacg tagtgctttt tcttgtctgc
     5581 gtaattgagc agcattttca aagtcttgtt tttggatcgc tgcttccttt tcttgtacta
     5641 gttcatcgat ttctagtttg atcacagttg attttgttaa gtgatcggtt tgatccaaac
     5701 ggactttcgc tgctgattca tcgatcaaat caattgcttt atcgggtagt tgacgagaag
     5761 tgatataacg aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg atctccactc
     5821 catggtgctc ttcatatctt gtacgtaatc cttgaagaat cacttctgct tcttcaggag
     5881 ttggctcatc tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat
     5941 atttttgata ttcatctagg gtcgttgctc cgatcgtttg taattctcct cgagctaacg
     6001 ccggtttcaa gatattcgaa gcatcaattg ctccttctgc tccacctgca ccaattaagg
     6061 tatgcaattc atcaatgaat aagattactt gaccatcatt atagatttcg tcgatgacct
     6121 ttttcattcg atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa gatcccatat
     6181 caagcatcat caggcgcttt tcttgcatgt cttctggcac ttcacctaaa atgatttttt
     6241 gagctaaccc ttcagcaatg gctgttttgc ctacaccagg ttcacccacc agtacagggt
     6301 tattctttgt ccgtcggcta agaatctgga taagccgttt cacttctctc gaacgaccaa
     6361 caactgggtc cagcttgttt tcttttgcta atttcgtcaa atcacgtgct aatgaatcta
     6421 gcgtaggtgt tccttgcggt gtttgccgtt tatcttgtac atttacattt ctacgacgat
     6481 tcattccttt tccagcagaa tcaccagcta gccccatttt ttgtttcaga acttgacggg
     6541 cttttgaaag gctgatacct aagttgatca tgattcgagt agccatgata ctttcatctt
     6601 ttaataagct catcaaaata tgttctgttc caactttttg agaaccagtt ttcttcactt
     6661 cgctgctagc taaagcaaaa acttgtttca ttcttggtga atatggaaga aagactcctt
     6721 tagggtaaga acggatacca ccataaccaa tcagatgttc aatttcttca taaatttccg
     6781 tttcattcaa atccatttca cgtaatactt ttccagcaat tccattttgc tcgaccacta
     6841 aggctagtaa aacatgttct gaactaactg attgatgttt caaccgtttt gcttcttctt
     6901 gtgcaatttc taatactgct tgggctcttt cagtaaaaag ttcttccatc taatctcact
     6961 tcccatcttc ataacttaaa cgttccagaa atgctcggag aatccgagca cgtgtatgat
     7021 cttcatattc attgaaattc aatgtgtttt ttgcaattgc actcaacatc aagtttcctt
     7081 ctttttttgt aattatttgg tcttcataaa gtttttgaat aatagcaaaa gcatttttct
     7141 cactgattgt atcatcaaat aactgattta tctgctcaag taattgtttt ttatctgaaa
     7201 tccttacttt tgcaattcgt atataaccac caccaccacg tttgctttct actagataac
     7261 cacgttggat ggtaaaacgg gtattaatca catagttgat ttgtgaagga acacagttaa
     7321 agaggtctgc catttcaatc cttcgaatct cgatcatttc actattttcc agaattttct
     7381 ttatatatga ttcaattaaa tccgaggtat tctgatgtgc catcttattc attccctttg
     7441 actaatattg accttaatta taacgaattt gataatagaa agaaagaaga acgctttttg
     7501 ttaaataaat cctttagaat caacaaaaaa aaatacggaa tatactattc cgtatcgacg
     7561 cgcccaagag gagtcgaacc cctaaccttt tgatccgtag tcaaacactc tatccagttg
     7621 agctatgggc gcatatttga aatgccgagg accggaatcg aaccggtacg gtgatcactc
     7681 accgcaggat tttaagtcct gtgcgtctgc cagttccgcc accccggcgc gattgctttg
     7741 gcaaagcgga aaacggggtt cgaacccgcg acccccacct tggcaaggtg gtgctctacc
     7801 actgagctat ttccgcgtgt ttaaagatgc cggctaaagg acttgaaccc tcgaccctct
     7861 gattacaaat cagatgctct accaactgag ctaagccggc atgtaaaact taaattatgc
     7921 gggtgaaggg acttgaaccc ccacgccgta aggcgctaga tcctaaatct agtgcgtctg
     7981 ccaattccgc cacacccgca aaaatatgag ccgtacaggg ctcgaacctg tgaccctctg
     8041 attaaaagtc agatgctcta ccaactgagc taacggctca tatggaggtt aacgggatcg
     8101 aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa cctccatgga
     8161 taacttaagc gtggcgacgt cctactctca caaggggcaa cccctcacta caatcggcgc
     8221 taagaagctt aacttctgtg ttcggcatgg ttacaggtgt atccttctcg ctatcgccac
     8281 cacactgtgg tgttatcttt tattgagtaa attttgttca ctcaaaactg gatttgaagt
     8341 cacataagtt tttttccgag ttcttttctt ttaacctatt ggttaagtcc tcgatcgatt
     8401 agtatcagtc cgctccatac atcactgtac ttccactcct gacctatcta cctgatcatc
     8461 tctcagggat cttactttct taaagaaatg ggaaatctca tcttgaggtg ggcttcacac
     8521 ttagatgctt tcagcgttta tcccttccct acatagctac ccagcaatgc ccttggcaga
     8581 acaactggta caccagcggt aagtccatcc cggtcctctc gtactaagga cagctcctct
     8641 caaatttcca acgcccgcga cggataggga ccgaactgtc tcacgacgtt ctgaacccag
     8701 ctcgcgtgcc gctttaatgg gcgaacagcc caacccttgg gaccgactac agccccagga
     8761 tgcgacgagc cgacatcgag gtgccaaacc tccccgtcga tgtggactct tgggggagat
     8821 aagcctgtta tccccagggt agcttttatc cgttgagcga tggcccttcc atgcggaacc
     8881 accggatcac taagcccgac tttcgtccct gctcgacttg ttggtctcgc agtcaagctc
     8941 ccttctgcct ttacactctt cgaatgattt ccaaccattc tgagggaacc tttgggcgcc
     9001 tccgttacct tttaggaggc gaccgcccca gtcaaactgc ccatctgaca ctgtctccca
     9061 ccacgattag tggtgcgggt tagagtggcc ataacgcagg ggtagtatcc caccagcgcc
     9121 tccatcgaaa ctagcgttcc gatttctacg gctcctacct atcctgtaca tgcggtacag
     9181 acactcaata tcaaactaca gtaaagctcc atggggtctt tccgtcctgt cgcgggtaac
     9241 ctgcatcttc acaggtacta aaatttcacc gagtctctcg ttgagacagt gcccaaatcg
     9301 ttacgccttt cgtgcgggtc ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt
     9361 tatagttacg gccgccgttt actggggctt caattcgtac cttcgcttgc gctaagcact
     9421 cctcttaacc ttccagcacc gggcaggcgt cagcccctat acttcatctt acgattttgc
     9481 agagacctgt gtttttgata aacagtcgct tgggcctatt cactgcggct gatcgtatga
     9541 tcagcacccc ttctcccgaa gttacggggt cattttgccg agttccttaa cgagagttct
     9601 ctcgctcacc ttaggattct ctcctcgact acctgtgtcg gtttgcggta cgggccgctg
     9661 ttttctcact agaagctttt ctcggcagtg tgacatcagg aacttcggta ctattatttc
     9721 cctccccatc acagcttgtc cttaagaaaa gaagcatttg actcctcttc agacttactg
     9781 cttggacaga catttccgat cgtctgcatt ccttagcctc ctgcgtccct ccattgctca
     9841 aacaaaaaca acgggtacag gaatatcaac ctgttatcca tcgcctacgc ctgtcggcct
     9901 cggcttaggt cccgactaac cctgggcgga cgagccttcc ccaggaaacc ttagtcattc
     9961 ggtggacagg attctcacct gtctttcgct actcataccg gcattctcac ttctaagcgc
    10021 tccag
//
LOCUS       DACIRY010000098         9970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.100, whole genome shotgun sequence.
ACCESSION   DACIRY010000098 DACIRY010000000
VERSION     DACIRY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.100"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IYE22_002056"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IYE22_002056"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ9507251.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IYE22_002057"
     CDS             complement(1174..1869)
                     /locus_tag="IYE22_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ9507252.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IYE22_002058"
     CDS             complement(1882..2598)
                     /locus_tag="IYE22_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ9507253.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IYE22_002059"
     CDS             2928..3533
                     /locus_tag="IYE22_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ9507254.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IYE22_002060"
     CDS             3858..4181
                     /locus_tag="IYE22_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ9507255.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IYE22_002061"
     CDS             4363..5733
                     /locus_tag="IYE22_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ9507256.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IYE22_002062"
     CDS             complement(5863..7164)
                     /locus_tag="IYE22_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ9507257.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IYE22_002063"
     CDS             complement(7394..9385)
                     /locus_tag="IYE22_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ9507258.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IYE22_002064"
     CDS             complement(9529..9795)
                     /locus_tag="IYE22_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ9507259.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2919 a         2069 c         1811 g         3171 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttataacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgat acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg
//
LOCUS       DACIRY010000099         9929 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.101, whole genome shotgun sequence.
ACCESSION   DACIRY010000099 DACIRY010000000
VERSION     DACIRY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.101"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            5..361
                     /locus_tag="IYE22_002065"
     CDS             5..361
                     /locus_tag="IYE22_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507260.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            377..727
                     /locus_tag="IYE22_002066"
     CDS             377..727
                     /locus_tag="IYE22_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ9507261.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            756..1535
                     /locus_tag="IYE22_002067"
     CDS             756..1535
                     /locus_tag="IYE22_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ9507262.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1546..2223
                     /locus_tag="IYE22_002068"
     CDS             1546..2223
                     /locus_tag="IYE22_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ9507263.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2242..3348
                     /locus_tag="IYE22_002069"
     CDS             2242..3348
                     /locus_tag="IYE22_002069"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ9507264.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3338..4429
                     /locus_tag="IYE22_002070"
     CDS             3338..4429
                     /locus_tag="IYE22_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ9507265.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4442..5182
                     /locus_tag="IYE22_002071"
     CDS             4442..5182
                     /locus_tag="IYE22_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ9507266.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5470..7410
                     /locus_tag="IYE22_002072"
     CDS             5470..7410
                     /locus_tag="IYE22_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9507267.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7497..9419
                     /locus_tag="IYE22_002073"
     CDS             7497..9419
                     /locus_tag="IYE22_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ9507268.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9699..>9929)
                     /locus_tag="IYE22_002074"
     CDS             complement(9699..>9929)
                     /locus_tag="IYE22_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ9507269.1"
                     /translation="LIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQA
                     VGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3239 a         1648 c         2205 g         2837 t
ORIGIN      
        1 aatcatgatt aaagtagtta ttgcagaccg tatgggtaaa ggacaaaatg tggccaaagg
       61 ggtagaagca gctggtggaa aagcagttgt cgtaccagga atgggggcag atatgcgttt
      121 gggcgatgtg atgcaacaag agcatgcaga tatgggtatc tctttttgtg gtagtggtgg
      181 tgcaggtgca ttaacagcag caacaaaata tggctatcca gaacgacatg gtatgcgttc
      241 gattgaagaa gggatcacag ctatcaagga tggcaaaact gttctaggtt tcggctttat
      301 ggatcaagag gaattaggga aacgtctgac agaagaattt atcaaaaagc atggaaaata
      361 gaaagaggga catgaaatga gcgttcaaaa gacaattcag caaaaagtta tcgtctccgg
      421 taaaggggaa acaaagcaac atgcttttgc atcagcgtta gcagaaattc aaaacaaaat
      481 aatggaacaa tcaaacgtat tattgcgaat cgaaccagtc aatgtggata ttttaaaggc
      541 ggtcgaaaac aaatacacag aacgctttct atttttcttc ttcccacggc aacgtattag
      601 ctatgaagtc gaactctctg ttactgtggc tgtaacagaa ataaaattgg cagacatatc
      661 gttcagccag caaatgactg aagatccaaa tgggattgcc attccatttg taactaaaaa
      721 aatctaattt tgaatcataa gggggaaaga ataacgtgga tttcgtaatc attctagtca
      781 aatcggtcat tatcggggga ttacttggtt ttgcagcatc tgtcggggca gctcgaatgt
      841 ttcatgcacc taatacacaa ggtttaggag cttttagaac attaggcgaa atgaatgcct
      901 gcatgggtga tccagcttct catttttcat ttggtttagg ctttttcttt aatgcatggg
      961 cttcttcagt tggtgctggt gcattcacac aagatgtgac gcatcgaatc attcccaatt
     1021 gggcagcggc agcacttcta gcaaaaaaca aagatgtcac ccagaccatg catgatccga
     1081 aaaaaatggg gatcattgga gcggttatcg gggcagtggt ggtagccttc ttaaacacaa
     1141 cagctgcagc aattccagag gcgttacaag tgacggcggt ttccgttttg gtaccagcag
     1201 cgacgattct gatcaataca gtaatgccag ttattttttg gttggcagct ttagacgcag
     1261 ggaaacgaac tggtttttgg ggcactcttt ttggtggtat cgcgcaattg attatgggaa
     1321 atgcggtacc tggtgttgtt ctaggaatct tgattggtaa aggcgtcgat gagagcggtt
     1381 ggaatcgcgt cacgaagagc atgatgatcg ctattgtttt attatttatt ttcagtggat
     1441 ttttccgtgg atttgatatg gaaatgatca agagcttcca gcttcaagtg cctaattggc
     1501 ttgataactt gcacaacatc tttagtgtga aataggagga gaatcatgga gacaacagaa
     1561 gaacaagcaa aagtattaga agcaaagcgg caaaagagct tttggtttgc agactggtct
     1621 tttccgctaa tcgtaggaat tatgtccgct gctgtatttg caggaacaca catgtatgtg
     1681 atgtatggtg tgggtgcatt caatgaagtt tcgatcgtgg cgatgttaaa agccgggctg
     1741 gatggcggat cctatggcgc agcggcagcg tttggtgcta gtttcttatt tgccagaatt
     1801 ttagaaggct ctttagttgg gatcttggac ttaggcggtt cgatcctaac tgggatcgga
     1861 attggggtac cagcaattct cttaagtatg aaaatagcgg caccaatcga taattttgca
     1921 ctctctttat tgactggttt ggttctaggt ttgattgttg gtggcatcat tgtcttgatt
     1981 cgtaaattta cgatcaatca atcaaattcc acttttggtg ctgacgtcat gatgggggca
     2041 ggaaattcat ctggacgttt tttgggtcca ttaattatat taagcgcgtg tgcagcatct
     2101 attccaatcg gcattggcgc tacattagga gcactaggat tctatgcttg gaaaaaacca
     2161 attgctggag gtgctatttt aggagcaatg attttaggag caatcttccc ggcagtaaat
     2221 taatagaaag agggtcatta aatgtttgat actttgatta aaaatggaag gttagttgat
     2281 ggatctaaaa tagaggtcgc tatagaaaaa ggcatgatta aagcagttgc agcagaaata
     2341 aatcagccgg cgcaagaagt agttgacttg gagggaaagt attatttatc cgctgggtgg
     2401 atcgatgatc atgtccactg ctatgaaaag atgaatcttt attatgacta tcctgatcag
     2461 ataggggtcg aaaaaggtgt gacgacggtc attgatgctg gaactacggg agcagaaaac
     2521 atccgtgaat tttatcaatt gacgaaatcg gtcaagacaa atgtgtatgc gctaatgaat
     2581 atctctaaat ggggcattgt caaacaagat gaacttgctg atctaacaaa gattcaagaa
     2641 gatctagtaa gtactgcgtt agcagaactg ccggaattta ttgtcgggat taaagcgaga
     2701 atgagtaaga cagttgttgg tgaaaatggc attaagccat tagaattagc taaaaaaatc
     2761 cagacagaaa acaatgatct gccattgatg gttcatatcg gttctgcacc tcctgagtta
     2821 gaagaagtat tgtcaagaat ggaaagaggc gatgtattga cccattgttt taatggaaaa
     2881 ccaaacggta ttttagatcc aaagacagat caaattaaaa actttgtctg ggatgcttac
     2941 aataatggtg tcgtctttga tattggacat gggacggata gctttaattt ccatgtggca
     3001 gaaaccgcac taaaagcagg aatgaaagct acctctatta gtacggatat ctacatccgc
     3061 aatcgtacaa atggcccagt ttttgatcta gcaactactt tagaaaaatt gagggtagtc
     3121 ggctattctt gggaagaaat cattgaaaaa gttacagcag ttcctgcacg gaattttcat
     3181 ttgaagaata agggccagtt agctgtcaat tatgatgcag atttgacgat atttgagatt
     3241 gttgaaaatg aaaaggaatt gaaagattcc aacggattta cacgtatagc taaagaacaa
     3301 attttaccga ttaaaacaat tattggaggc gtcatatatg acaattagct atggaaagtt
     3361 tgatttaaaa gaagtcatta atgcatcggg aaaaatgacg atcctagggg tttctaaggt
     3421 atctgaaagt gtcctagcag cacaacgatt tggaggcgag catttttttg agatgagtga
     3481 tcttgccatc aaaaccggcg cttatctttc tcaattactg aaagtggaag atgcgcaaat
     3541 cgtttcttct gcttcagcag ggattgctca atctgttgct gctttaatcg gacagggcga
     3601 catgtaccat gtctatcatc cctatacaga aaaaatcgct agacgggaaa tcatcatacc
     3661 taagggacat aatgtagatt atgggacacc ggttgaagta atggttgctc aaggtggagg
     3721 aaaagtcata gaagctggct atgcaaatat gtgtacaccg gaacatgttt ccatgatgat
     3781 tacggaaaaa actgctgcgc ttctatatat taaaagtcat catgcagttc agaaaagtat
     3841 gctgacggtg gcagaaatgg tagaagttgc caaagctcat cgtttgccat tgatcgttga
     3901 tgcagctgcg gaagaagatt tattcaaata tagcgaaatg ggcgttgact tagtgattta
     3961 ctctggggca aaagcaatcg aaggtccgag tgccggactg gtcataggaa aaaagaagta
     4021 tattcaatgg attcggctac agtctaaagg tattggccga gcaatgaaaa tcggtaaaga
     4081 taatatttta ggctttaccc aggcactaga agattattta aaaaatggga gcgaatccgg
     4141 tgactccatg aagcaacgat tagcgccttt tgttaacgca atcaatcaac ttcctgacct
     4201 ttcagcgaaa atagttcaag atggtgccgg cagagatatt tatcgagcaa gtgtaacgat
     4261 taatggcgat aaaagtgcga aagaagtcat tcaggaatta aaagcacgaa atccagcagt
     4321 ctacacccga gaatatcaag caaataacgg aatcattgag ttcgacattc gttccgtcag
     4381 ccaaaacgaa atggaaaaaa ttataaaaag attgagagaa atcatgtagg aggcttttaa
     4441 attgtcagtt atcccaaatt attataaaaa tcgtgtatgt ttaaatgtat tagctcattc
     4501 agttgaaaat gcaaaagcat gctatgaagc agcggaaggg catgtcgtac taggtgtgct
     4561 atcgaaaaat tatgctagcg atgaagcagc aatcgaagat atgaaaaggt accaagacgc
     4621 aaccaataat gcgttgtcag ttggattagg cgctggagat ccaaaccaaa gtcagatggt
     4681 ttcgcgttta tctggggttt tacaaccaca acatgtcaat caagtattta ctggcgtggg
     4741 gacttctcgt gctttattgg gccaaaatga tacagtcatc aatggattgg tttcaccaac
     4801 aggtaagatc ggctatgtca atattgctac aggtccgtta agttcacaag cgccagcagc
     4861 tgaagtgcct attgaaactg ccattcaaat gctgcaagac atgggaggta cttcaatcaa
     4921 gtatttcccg atgaaaggct tagcacatag ggaagaattt aaagcagttg cggaagcttg
     4981 cgcaaaatat gaattctatt tagagccaac aggaggaatt gatctttcaa actttgaaga
     5041 aattctgaaa attgcaatcg atgcaggtgt aaaaaaagtg attcctcatg tttatagttc
     5101 aattatagat aaagaaacag gagatacgcg tataggagac gtaaagatac tattaagcat
     5161 ggtaaaaaaa attattcact aactcattag aaaataagaa gcttattcca gaatgaaatt
     5221 aaatggaata agctttttat aattaattac ggaaattgtt aatgtagata ataatttaga
     5281 tgtacgtaag cagaagtaca gaaatgtaag attttccaat attattttat tcaacataat
     5341 aaatcgtata tcaatttttt tagcgaaatt tctatttatt ccaagaattt cagttattac
     5401 ggaatcattt tgtgcttaat aaaaagaaac cgtttttata tactataaat gtagaaaggc
     5461 ggtaggaaaa tgaaaaaaat tattgcaatc acatcctgtc cagttggtat tgcccataca
     5521 tacatggcag cagaaaactt agagaaagcg gggaaagcag ttggcgccga ggtaaaagtc
     5581 gaaacacatg gttccatcgg tattgaaaat gaattgactg caagagatat cgaagaagca
     5641 gctggtgtaa tcatcgcagc agatactaaa atagataaat cacgattcgg cggtaaacct
     5701 ttgataaccg taggtgtgca agaaggaatc caccatgcag atgagttggt gagagatatt
     5761 ttagcaggaa aagcgccagt ctataaaagt acggaggcaa ttatttcttc cgaaaataaa
     5821 agtgaatcag aaaatttagg taaaaaaatc tataagagtt tgatgaatgg cgtttcgtac
     5881 atggtacctt tcgtagttac aggagggtta ttgatcgcga tttctttaac attaggagga
     5941 acagctacac ctgaagggat caagatacca gaaggaacga tttgggcaac gatgaatagt
     6001 atcggaagta tcgctatggg gttgatggtt ccaattctat ctgcatttat tgcgcaaagt
     6061 attgcagatc gtccaggact tgttcctgga tttgttggtg ggatgttagc ggcaaacgga
     6121 gcattatatg gtagtgatgc gaatgcggga ttcttaggcg ggatcatcac tggattttta
     6181 gcaggattca ttgctttagg aatcaagaaa gtgcgagtac cgaaagcttt acaatcgatc
     6241 atgccaatta ttgttatccc aattttagga tcattggcag ttggttttgt gtttatctat
     6301 gttattggag aaccagtagc tcttctattt agttcattga ctcacttttt agcagggatg
     6361 caaggaggaa gtgaaattgt tctagctatg atcttaggag cgatgattgc ttttgatatg
     6421 ggggggccgt tcaataaagt agcctttttg ttcggatctg gcttgattgc agaaggcaat
     6481 tattccatta tggggccaat tgctgtagct atctgtatcc ctccattagc attggggctg
     6541 gcatcattga ttttaaaaac caaatttacg aaaacagaac gagaagctgg taaggcgtcc
     6601 ttagcaatgg gactgtttgg gattacagaa ggcgctattc cgtttgcttc aacagatcca
     6661 ctacgggtga ttccaagcat tgtaatcggg gcaatgacag gaagtgtgat cgcgatgctt
     6721 tcaggagtga cagatcatgt agctcatggc ggtccgatcg tagcggtttt aggagcagtg
     6781 gatcacgtag ccatgttttt tgtagcagtg attgctggta ttgctgtaac cgtgctaatg
     6841 ttaagaatcc ttaaaccgac attacaacaa actgcggttg ctgcagcaaa tggtgtgaca
     6901 gtagcagatc tatcagagac tgaaacaagt gatgtttata cgaaaactga aacttttgat
     6961 tctatcacgg atattatgtc agatgaagca atcgttttag gtgaaacagc aacaacaaaa
     7021 gacggagtaa tcgaagattt agtccaacgt ttagaaaaaa cagcggcgat cacagataaa
     7081 accagattta aagaagtcat ctatgataga gaacatgaaa gtacaacggg aatcggtatg
     7141 gggattgcta ttccacatgg aaaatcagct tatgttctta aaccaacagt ggcatttgcc
     7201 agaagtcaat ctggggtaga ttggcattct ttagatggaa aacctgcaca tatgatcttt
     7261 atgattgctg tacctgaaaa cagccaaggt gatatgcact tgaaaatact gcaacgtcta
     7321 tcacgtaaat tgatggatga tgacttccgt caagcactga tggaagcgcc agataaaaca
     7381 acagttcacc aattattaag cgaaatgtaa cataagatga aaaatcgacc gtgatgagac
     7441 ataatagatg tgttatcatg atcgattttt tatttagaag ggagtttctg cacattatga
     7501 atcagcgtca gcaatctttg atctatcaat taagtgagaa caataattat ataacaacaa
     7561 aaaagctagc agagcatttc catgtgtcag aaagaacgat tcatagcgat ttgaatacca
     7621 ttgaaacttg gttgagagac aaacatctgc ctttaatgtt tgaaagaaaa aaaggtacag
     7681 gtatcttgtt gaatggtctg cctcacgaaa gggaacaact gaaacgagcg cttaatgaag
     7741 agactcaaac agaaatggat aaaaaacagc ttcgtcagtt attgattttc catctgctag
     7801 tagcaaaaga cggatttagt cttgaagaac tttcagaaaa actatatgtt ggaaaacgta
     7861 cgattcgtaa agaattgaca gagttgcaaa gcttttttga gtatcaccat ttacagctag
     7921 tatccaaaac aaaactagga acttttctta aaggcgatga acaagaaaaa agacagttgc
     7981 ttgttaagac attacgaaat atgcagaaag aagatccaca aagtccgtcg ttgaaagaat
     8041 tcttcgctaa agatacatta aaagttatcc aacatacatt acaagatgtt ttttatgaaa
     8101 accatttaga acagccgaat ggactggcta gtgtggagat tcacatctac tttatgctcg
     8161 agcgaatgaa acagtaccag aaagtgaaat taagtaaaga cgaaaacgaa gtcgtggaac
     8221 acacccaagc acaaaagtta agtagtcaaa tattagcaaa actagccacc atctatccaa
     8281 ttgaattttc tcctgatgaa atcaattatc tggctttgcg tatcgccaat ctttttacaa
     8341 attcacaagc agccacacgt tttcaaaagg aatctagcgc acttgctgat catttgatcg
     8401 aacaagtgga acagtttcta ggctattcat taaaagaaga tcagctacta aaacaaaatt
     8461 taaggtctca tttatcttca acttattttc gactccatta tggtttgcag atttctaatc
     8521 cattgacgaa aaatgtgttt agtacgtaca cacaactttt cttagtttta cagttaattt
     8581 tagaagatta ttttgaaaag gaaaattttt atgttccaca agaggagaca gcttatttaa
     8641 cgatccattt ccaagcagcg atcgagcgac acaaacatca gaaaagtaga agatatcaaa
     8701 cggttttagt tagtgaatat tcaaaagcta tggctacttt tctcgaagca cggctgaaca
     8761 gagaacttcc agaattaacg attatggatc tagttgaata taagccagaa ttaaagagac
     8821 aagattttcc ctctgttgat tttatcttgg ctacgttacc atttaaacat ataagcattc
     8881 cagtaatcga gatctcacca atgatcacag aaactgatct agcctatttg acaaaatata
     8941 tgctggagca tgtgcctatc aagaagaaaa aaacatttga tctagcaagc tttactcatc
     9001 catttctgat ctttcctcaa cttgaacgga cagacccagc cgatatttta aactttatgg
     9061 gaaacgtctt agtggaacat cattatgtgg agccggaatt catcgattct gtcttggaaa
     9121 gagacagtca ctccagtact agagtagcac catttgtaac aatccctcac ggtaatccgc
     9181 tgtatgtgaa gcattcgatg atttctattg ctacaatgaa agaaccagtc ctatggcacg
     9241 gggaacagat acgtatcgta ctgctattag caataaagaa aaatgatttg aaacatgcag
     9301 agttcaaaaa aatattttct gttattcact atctagaaaa gcagccagaa caaatggctc
     9361 agttgatgca atccaatcat ccgctagaaa tcttaacgtt gttatctagt tatgaataag
     9421 tagcgattaa aaaactgcct ttatatttat aataattcta tctatttatt tggaagtaga
     9481 aaactaaata caaaaaaaga ggatgtggcc aaagttgtgg cacatccttt tattttgagt
     9541 ttcttataat tttttatagt tgcttacgat gtcttgacta ctggtaaacg gtgttcaaga
     9601 gacaatttgt cggcaataag gcacaatttt gtaaaatatg gaaagctatt tttttaacat
     9661 tgtttcttag tgcttgaaaa atcattttga atacttcttt atacgatcga tgtacgttga
     9721 tttaagtcta aaacttttct tttgattcct tctctgactt ttctgaattc cgttaatatt
     9781 tcttcttcgc tcccgacagc ttgtgcagga tcttttaggt cccagtgaag gtgttcgtga
     9841 ttgtttggaa cggcagggca cttgtctttg gcatcaccac aaagtgtaat gatcaagtct
     9901 gcctgagtaa agtaatctct atcaatcaa
//
LOCUS       DACIRY010000100         9927 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.102, whole genome shotgun sequence.
ACCESSION   DACIRY010000100 DACIRY010000000
VERSION     DACIRY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.102"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(129..833)
                     /locus_tag="IYE22_002075"
     CDS             complement(129..833)
                     /locus_tag="IYE22_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9507270.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            964..1467
                     /locus_tag="IYE22_002076"
     CDS             964..1467
                     /locus_tag="IYE22_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ9507271.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            1427..1765
                     /locus_tag="IYE22_002077"
     CDS             1427..1765
                     /locus_tag="IYE22_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ9507272.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            1786..3204
                     /gene="ffh"
                     /locus_tag="IYE22_002078"
     CDS             1786..3204
                     /gene="ffh"
                     /locus_tag="IYE22_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ9507273.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            3316..3894
                     /locus_tag="IYE22_002079"
     CDS             3316..3894
                     /locus_tag="IYE22_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ9507274.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            3898..4419
                     /locus_tag="IYE22_002080"
     CDS             3898..4419
                     /locus_tag="IYE22_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ9507275.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(4498..5052)
                     /locus_tag="IYE22_002081"
     CDS             complement(4498..5052)
                     /locus_tag="IYE22_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ9507276.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            5305..5580
                     /gene="rpsP"
                     /locus_tag="IYE22_002082"
     CDS             5305..5580
                     /gene="rpsP"
                     /locus_tag="IYE22_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ9507277.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            5592..5843
                     /locus_tag="IYE22_002083"
     CDS             5592..5843
                     /locus_tag="IYE22_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ9507278.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            5968..6759
                     /locus_tag="IYE22_002084"
     CDS             5968..6759
                     /locus_tag="IYE22_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507279.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            6929..7450
                     /gene="rimM"
                     /locus_tag="IYE22_002085"
     CDS             6929..7450
                     /gene="rimM"
                     /locus_tag="IYE22_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ9507280.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            7440..8201
                     /gene="trmD"
                     /locus_tag="IYE22_002086"
     CDS             7440..8201
                     /gene="trmD"
                     /locus_tag="IYE22_002086"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ9507281.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            8337..8684
                     /gene="rplS"
                     /locus_tag="IYE22_002087"
     CDS             8337..8684
                     /gene="rplS"
                     /locus_tag="IYE22_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ9507282.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            8942..9235
                     /locus_tag="IYE22_002088"
     CDS             8942..9235
                     /locus_tag="IYE22_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ9507283.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         3443 a         1752 c         2011 g         2721 t
ORIGIN      
        1 tgtcaggtag ctttcttgct gttccacgat ctgctgttga aaataatggg aaatcaattg
       61 ccagccacca aaaaacaaac taactaaaag aataagaaag aagagacgtt cataaatttt
      121 tttccatttc atcgtttggg ttcctgaaac ttatagccaa atcctctaat agtcaccaag
      181 tactttgggt gttttggatc tcgctcgatt ttttctcgca aatgactaac gtgtacatct
      241 acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg
      301 tcgatcacac ggtcttttcg tttcatgaaa taaacaagta attcaaattc tttcggtgtt
      361 agttcaatca gatggtcgtc tacggtcact tgataattgg tcaaatcagc agtgatttgt
      421 ccaatcgata gatattcagg ctcagcttca tcttttgatt gcttacgagg ttcaattcgt
      481 ctgaatattg ctttcatacg ggctaatact tcgcgaggac taaatggttt ggtcaaatag
      541 tcgtcagcac ctatttctag gccaatgatc cgatccactt ggtcatcttt agccgtcagc
      601 atcaaaatcg gtgtttcatt tttttcttga cgcagccgct gagtgatctc catgccatcc
      661 atcgttggaa gcatcacatc taaaatgatg aaatcaaatg attgttctaa tgctaattct
      721 aatccttcgc ctccatctgt ggcactggtt accttgtagc cttctttttc caaattgaac
      781 gtcaagagcg ttacaataga aggttcatca tcaacaacta gtattttttt cattcaaatc
      841 tcctttaaat aggtttcatt ctgtttatat tttagcatag aatcagtaca aagggctatt
      901 ttacaaaaga aataaatggt ataataggaa ggcactttgc tgaatagaaa ggaagagcag
      961 tcaatgattg gaatcagtgc atgtttaggc ggtctggcct gtcgctatga tggacagaaa
     1021 aaagaagtac cagaattgat ggaactagtt gaaactggag aagctgtcat ggtctgtcca
     1081 gaagtgatcg gcggattacc aattcctaga gatcccgctg aaatcattgg tggaaatgga
     1141 ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa
     1201 gcgtacaaaa atggcgcgat caaagcctat caaaaattgc aggaaaaaca cattgatcta
     1261 gtcatcatga aggaaaaaag cccttcatgc ggaacccatt cgatttatga cggaagtttt
     1321 tccggaataa aaaaaaatgg tgccggagtg gctactgctt attttattca gcaaaaaatg
     1381 actgtgctct ctgatgaaga atggctgaaa actagaggtg agcagaatgg aaatcgaaaa
     1441 gaccaatcga atgaacgcgc tctttgaatt ttattcgacc ttattgacag aaaaacaaat
     1501 gaactacatg gaattatatt atgcagatga tttttcatta ggtgagatcg cggaagaata
     1561 cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg acaagcaaga tcttggagga
     1621 ttatgaaaaa aaactccatc ttttttctaa ttatattgtt cgagaacagg tattggaaaa
     1681 tatgactgca tatattacag ataagtaccc ggaagataaa aaactacttg aatatgtaca
     1741 gcaaattcaa gcaatcgaag aataaataaa aggaatgaag aaaatatggc atttgaaagc
     1801 ttaactgaac gtctccagca ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa
     1861 gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct tactagaagc cgatgttaac
     1921 ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg
     1981 ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc
     2041 acacttggtt ctgaaacagc agagttaaat aaatcaccaa aaataccaac tgttatcatg
     2101 atggctggtc ttcaaggggc aggtaaaacg acctttactg gtaaattagc caattattta
     2161 aagaaaaatg aaaatgctcg tccgctccta atcgcagggg acgtatatcg accagctgcg
     2221 atcgaccaat tgaaagtatt aggtcaacag ctagacgttc ctgtatttga tatgggaact
     2281 gaagtcagcc cggtagagat cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac
     2341 gactatgtat tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt gatggatgag
     2401 ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt tgcttgttgt cgatgccatg
     2461 actggtcaag atgccgtcaa cgtagctgat agcttcaacc agcaattagg tattacaggg
     2521 gtcgtcatca ctaaacttga tggggatact cgaggcgggg cagcactatc gatccgctca
     2581 gtgacaggcg caccaatcaa attcatcggt tctggtgaaa aattgactga cttagaagta
     2641 ttccacccag atcgtatggc tagccgtatc cttggtatgg gggatatgct gacattgatt
     2701 gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg
     2761 gaaaattcat tcgatttcaa tgactttatt gaacagcttg atcaagtgat gggtatgggc
     2821 ccactggaag atctaatcaa aatgatccca ggaatgaatc aagtccctgg aatcgaaaac
     2881 gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga tggtttattc tatgacgcca
     2941 gcagaaagag caaatcctga tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt
     3001 tctggaaaca gtgttgttga agtgaatcga atgatcaaac aattcaagga atcaagaaaa
     3061 atgatgcagc aaatgtccaa aggggatatg aacatcccag gtatggacca aatgtttggt
     3121 tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag
     3181 aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt
     3241 ctactgccgg agcttttcta tcagcaattt ttattcttta ctaatacaat tactctaaac
     3301 gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat
     3361 catgatactg tctggggtgt tcctgaatat gatgaccaaa agctgttccg aaaattgatg
     3421 cttgatatta atcaagccgg attgagctgg cagacgattt taaataaaag tgaagcattc
     3481 gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc
     3541 agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc
     3601 caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc agagtatctt
     3661 tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc attcacaaga agaagtccca
     3721 acaacaagtc ctctttcaga tgaattagca aaagatctaa agaaaagagg atttaaattt
     3781 atcggaagca caacaatcta tgctttttta gaagctgtcg ggatcatcaa tgatcacctt
     3841 gacagttgtt tccgcaaaga agaggtcgta cgtatcggca gaaagggcga ataaagcatg
     3901 cgaaaaatca tattttatgg tgctataagt ttagatggct atttggcaac gaaaacggat
     3961 gatcttcaat ggctttttga cactccaaca ggtaagcaaa ctacttatga agctttttat
     4021 gaaacaatcg atacgaccat catgggcaga aaaacttatc aggaagcaaa aaaatatttg
     4081 gagaccgaag caatttatcc agagaaaaag aatttcgttt tttccaccca cgcacaatta
     4141 caattgccag atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa
     4201 atgacagaag gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa accgctattg
     4261 gaacatcaaa tgattgatga atggtatatc cagattaccc ctgtattatt aggggacggg
     4321 attcctttat tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga cacgacacga
     4381 ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga agtaaataaa ataaaaaagg
     4441 caactatatg cagaaatgtc ctgtacatag ttgccttttt tattaccgtt atgcttttta
     4501 ctctgttaat ttgttagaaa aatctagata cgcatctaaa atcgtttgga agaaacttaa
     4561 tgttccttca acgatattac cattttcatc aattagattc gcaacgtttc cgatatatgc
     4621 ttccggttgc tgcaaggttg gcacattcaa aaagacaagt gattggcgta gatggtggtt
     4681 tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg actaaacctg gtttatgatc
     4741 ccaaacactg tgtccataag gtcttgagcc tacatctaaa gcatttttca atactgctgg
     4801 cacagaacgg ttgtattcag gagtaacgaa aacgacaccg tctagtcccc tcacttcctc
     4861 acgaaaacga gtccattcag caggagcctg ttctggagtt tcgagatcct cattgtaaaa
     4921 tggcagatca tcgattttta caaatacact ttcgtaccct tcaggaagca atttcgcaaa
     4981 agcttctgct acttttttgt tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat
     5041 tttttttgtc atacgatttt tcctccttca aactatctat aataatctta caaaagataa
     5101 gtaactggag caaataaaaa cacttactga caaaagctac atagaagata gattacttat
     5161 ttttaacaaa aaagaagtct aaaatcttct attttctaaa aaaaatcgaa tttaatcaag
     5221 ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt tacactacat cttgtgaata
     5281 aaacaatgga ggtggaaata aataatggca gttaaaatcc gtttaaaacg tatgggttct
     5341 aaaaagagtc cgttttaccg tatcgtcgta gctgattcac gttctcctcg tgatggacgt
     5401 ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa
     5461 gaagacttag ttcttgactg gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac
     5521 atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc aaagaaataa
     5581 ggtgaccgga tatgaaagat ttaagcgact tagtcttaac aatcgttcgt ccgctagtca
     5641 cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa agacttttac gagtacaatc
     5701 taacagtggc tcctgaagat attggccgta tcattggaaa acaagggcgt gttgctaaag
     5761 caatccgcac aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata
     5821 tcatcgatga taaggaaaag taaataaacg tgctgtactc gaattttcga gtgcagcttt
     5881 tttgtttttt ttattaattg aagaaatgat tatttggcaa aacaagcaag aaagcgtatc
     5941 cttataggag gagggagtga acagccaatg agaaagaaaa tgttatttat tccatttgct
     6001 ctattgttaa gcgcttgtag ttcggttact caagaatcag acaatacaca tctatcaagc
     6061 agtatatctg aaactgttac cagtccagaa aaaagtagta ccaaaacggc ttccaccact
     6121 gaacaaacaa tggctacctc aaataatgag aaaaagactg ctttagacca gttgaaagaa
     6181 caacagccaa atgttcccat gcctctagat gtaccagtct cttcaggcta tctcaatatc
     6241 gcagcgacac atacaaagca aggttattct attttatatt acagaacaga tcgtcccctc
     6301 gggttgaatg ctgacgaatt gaatcaagaa acgcctattg caacctatct atatcaatat
     6361 gggtttgctt ctagtcaaga aacaatccaa gttttgcagc catttgagat cgacacaaat
     6421 ggtcagcaag tggacttagg ttctcgaatc acagggtatc agcaaggtgc agcaggttca
     6481 agttttcttg aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa taatatagaa
     6541 ggccaagatc ccttactatt agctaaagaa atcgtagctt atttggaaga aaacagtcta
     6601 ccagcacctg aacaatttgg taaaattact gttgatatgg gtgatacgac gaatcgagcc
     6661 gtagaagtaa gctggcaaga accgaaaaat gcctatacga tcactcatca agatcctatg
     6721 tctgcgttga agatggcagt ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc
     6781 ccatcattta actccaagtc acaggaagta atcaaaaata gcacgccgca tttttgcata
     6841 attttggctt aataccttaa aagaaaacta gaattgcctc ttcacacatg ctaaaataaa
     6901 acagacaaaa aagaaatggg gatataagtt gactgaatac ttgaatgtag gaaaaatcgt
     6961 aaacacacaa ggtatcaaag gcgaagtccg tgttatctca acaacggatt tcccagaaga
     7021 acgttacaaa aaaggaacgg tgctaacact attccaagac ggaaaagcac ctgtggaatt
     7081 aacagtcaaa agtcatcgca aacataaaaa ttttgactta ttgagttttg aaggacatcc
     7141 ttctatcaat gatgtggaaa aatatcggga tggcatatta aaagtgtcaa aagacaactc
     7201 tgtagatcta gctgaaaatg aattctatta ccatgagatc atcggtctgc atgtattaga
     7261 agatggaact gagttaggta aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg
     7321 ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca tatatcgctt ctgtcgtcaa
     7381 agaagtcgat ttagaagctg gagtggtccg tgtagaaatt ccagaaggac tgattgacga
     7441 tgaagattga tgtattgact ttatttccaa gaatgtttga aggtcctatg ggggaatcca
     7501 tcatcggtaa ggctgtagat aaaggattat tagatatcaa tatctctaac ttccgcgatt
     7561 attcagataa caaacatcag acagtagatg attatccata cggcggtgga gcaggtatgc
     7621 tgctcaaagt ccagcccatc tatgataaca tccaagccat tgaaaaagct gcaccggatg
     7681 tcaaaaaaag agtaattctt cttgacccag ctggtaaacg attcgatcag aaaatggcag
     7741 aagaattttc acaagaagaa cacttagtat tcatttgtgg tcattatgag ggatatgatg
     7801 aacgtatccg cacattagtg acagatgaag tttccttagg agattatgtc cttactgggg
     7861 gagaattagg tgcgatggtc atgatcgatg caacagttcg gcttttgcca gatgtgttag
     7921 ggaatcagac ttcagctcaa acggattctt attcaaccgg tttattggaa catccccaat
     7981 atactcgccc ggcagaatat aaagggatga aagtaccaga agtattgaca aatggaaatc
     8041 ataaattgat tgaagaatgg caattaaaag aatcactaag aaggacctat caacgtcggc
     8101 ctgatctttt ggaaacactt gaatggacac ctcaaatgac gaaattttta gaagaaatca
     8161 agaaagaaga acaagaaaaa acagcagatg aagccaagtg actttttcta aaatcttctt
     8221 tacagtaggt tgataatatg ttagtatttt atggtgagtg caaaagcact taactattac
     8281 gatattccgc tgtgagaaaa gctcataaga atatttggaa taaggagaag aaaaccatga
     8341 atccattaat cgaagaatta acaaaagaac aattacgttc tgacatccca gcgttccgcc
     8401 ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt
     8461 tatttgaagg tgttgttatc aaacgccgcg gtgctggaat cagcgaaact tatacagtac
     8521 gtaaagtttc taacggagtg ggcgttgaac gtacattccc attgcataca ccacgtgttg
     8581 caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg
     8641 cattacacgg aaaagctgct cgtatcaaag aaatccgtcg ttaatcattg ttatcagact
     8701 gagaaagaac ccttggtatt caagggttct tttttcttag catgtaattt gaacaccctg
     8761 atccagtttt ttctttaatt attggtaggg gagaataatg gattagaata aatctaccta
     8821 taagtagtag taaaaaaaag agcactatga taatatcttt cttaagagta accgctttaa
     8881 taaaaaatcc agaatattct aacataaatg gagtattctt agaatcaaaa ggagaaagaa
     8941 gatggtttat acttatacgg agcaagaaaa gatgaaagtt ttagacacat tcatgaatga
     9001 tggaaaagta aaacaagtac caagttcaac aaaaaagaaa tacatcctat tgaaagaagt
     9061 cttgaaacgc tttgaccatg gagtgactta tacagaaaca gaggttaatt cgatactgct
     9121 gaacgtattt tctagtggcg attatgtcga acaaagacgt tatctaatca catttggctt
     9181 tttcaaaaga agttcagacg gtcgggcata tcagatgatg ggaattgaaa attagaagtt
     9241 ttaatttata ctcattgaaa aaagttcaag tatgcgatat tggttctaca ccgcatactt
     9301 gaactttttt tgtcatctgt atctataata caaagccaag aacgttattc aaaatatgga
     9361 tagctatgat aaaagctaat gagcgattcg cagttaaata agcaatcgtc agtaaaatcg
     9421 ttccaggagc gtacatgaaa aaatcaattg gatgcgttac atgaataaag ataattctct
     9481 tttatttgta cgaataaata ccaaaacgta cctcctaaaa ttagattttg gacagatgcc
     9541 ctttaaaaag ataacaagat ttcgttcttg aaacaatata tatgctttta ttccaatgag
     9601 gttgtgaaaa cagtattttg tcctgagcgt tagaaaattg aaaatgggga atagcaactc
     9661 gtactttacc attttttaag tgaatacaag atcagctaat tgaacaccgt ttattcgtga
     9721 tgagggacac aacactgcag tcgacacatt tctcttttat gttcaaagat aacaaatatt
     9781 taaaacaaag aaaaatagta gataatcccg tcctaactct tttattcttc cctcatagct
     9841 tccaaattta agcaatactt caattaatca agttttatct ttttttttca ataatcgtaa
     9901 aaaaagagta ttttttattt aaaaata
//
LOCUS       DACIRY010000101         9848 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.103, whole genome shotgun sequence.
ACCESSION   DACIRY010000101 DACIRY010000000
VERSION     DACIRY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.103"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(237..1085)
                     /locus_tag="IYE22_002089"
     CDS             complement(237..1085)
                     /locus_tag="IYE22_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9507284.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1248..2150
                     /gene="gnd"
                     /locus_tag="IYE22_002090"
     CDS             1248..2150
                     /gene="gnd"
                     /locus_tag="IYE22_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ9507285.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2152..3153
                     /locus_tag="IYE22_002091"
     CDS             2152..3153
                     /locus_tag="IYE22_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ9507286.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3150..3434
                     /locus_tag="IYE22_002092"
     CDS             3150..3434
                     /locus_tag="IYE22_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9507287.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3467..4264
                     /locus_tag="IYE22_002093"
     CDS             3467..4264
                     /locus_tag="IYE22_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9507288.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4278..5111
                     /locus_tag="IYE22_002094"
     CDS             4278..5111
                     /locus_tag="IYE22_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9507289.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5129..5986
                     /locus_tag="IYE22_002095"
     CDS             5129..5986
                     /locus_tag="IYE22_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9507290.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6008..6517
                     /locus_tag="IYE22_002096"
     CDS             6008..6517
                     /locus_tag="IYE22_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9507291.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6554..6955
                     /locus_tag="IYE22_002097"
     CDS             6554..6955
                     /locus_tag="IYE22_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507292.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7067..7420
                     /locus_tag="IYE22_002098"
     CDS             7067..7420
                     /locus_tag="IYE22_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507293.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7454..7897
                     /locus_tag="IYE22_002099"
     CDS             7454..7897
                     /locus_tag="IYE22_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ9507294.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7914..8999
                     /gene="uxuA"
                     /locus_tag="IYE22_002100"
     CDS             7914..8999
                     /gene="uxuA"
                     /locus_tag="IYE22_002100"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ9507295.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8992..9705
                     /locus_tag="IYE22_002101"
     CDS             8992..9705
                     /locus_tag="IYE22_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ9507296.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3281 a         1557 c         2170 g         2840 t
ORIGIN      
        1 ataataaaaa aaagaaaaga taaatttttt actactgtat actaactttg ttatcgcatc
       61 taacaagtaa ctcgaataat accaatacaa ccttctccaa agatgcgatg acatacgttc
      121 taacccaccg actattgatg ttctgtacca cccttgcaga accaaaaaag ccactaccaa
      181 atatttggta gtggcttttt ttgttttatg gatttttcgc tttttctgtt atggtgctat
      241 tctttgattt cagaaatagt atgtcgaact ttagcgatag aacgacgtga ttctttttcg
      301 ttgtaaaaca tcaaaatcac atatagagag tccaaaatgc ttagttgaga aatacgggaa
      361 gctaaagctt cagatcggaa ttctgtttct tcagaaatag aaataaaaac gacatcgcca
      421 agtttagcaa gggaagaatt agcttggcta gtaataacga ttgtttttgc tccagttttt
      481 cttacggttt cagcaatatg gattgtttct tttgattgcc ctgtgtgaga aatcaaaaca
      541 gcacagtctt tttccgtcaa taaagaagct tccatcaatt gaatatggta atccgtgctg
      601 tgagaagttg cgatagggga acgcaaaaat ttatgataag cgtcagttgc tacaatctct
      661 gaaccgccaa caccgaaaaa atagacacgt ttagattcat tgataatagt agccgctttt
      721 tttagatcct tttgttgtaa caactttttt gtgtcattta aagttgaaat actagactcg
      781 aacacctttt gcgccatagt gagctcagta tctgttttct gaatattttc gtggatagaa
      841 atagcagata gatcattttc ttggatcaac atggccattt taaaatcttt aaatccatta
      901 taaccaagtt tttttgtaaa ttgaaaaaaa gtagagtcag caatgcccag ttcacttgct
      961 aaattgctaa taggactatg acttactttg cttggatttt ctaaaatata atcggcgata
     1021 gcctgttctt ttgggctgaa gtcgttgtac attgttttga tttgtagtct tatggagacg
     1081 ctcatgagaa ttcctcattt tcataataag attatcattt tttccgttct cattaatgtt
     1141 aaagatacaa ggagagtagg aaaaaatcaa acaaaaaata tttttgtaaa atctatttac
     1201 aaaaaaattt tttgtataat gagggtataa gaaatggagg cgctatcatg gaaattggtg
     1261 taattggttt gggaaaaatg ggattgaatt tagctttaaa cttaaaagag caccatcaca
     1321 aagtacaagg actcgacatt tcaagtacag ctgttagtca ggcaagagaa aatgacattc
     1381 ctgcttatca agagatctct gaatttctag ctacttttga gcagaaaaaa atcatctggt
     1441 taatgcttcc tgctggagag ataaccgaaa acatgttaag aaaacttttt ccaaaactcg
     1501 gaccaggaga cattattatt gaaggcggaa actcttatta taaagattca atccgtcgag
     1561 cagaagagtt tcagaaaaat gaaatcggtt acttggattg tgggacttct ggaggaatcg
     1621 aaggagcacg acatcaagcc tgcttaatga ttggaggaga taaagaaact tttctatccg
     1681 tggaacaatt attcaaagat gtagcaattg aaagaggata tctgtatgct ggtgcatccg
     1741 gaagcggaca cttcttgaaa atgatccata atggaatcga atatggaatg atgcaagcaa
     1801 tcggtgaagg attccaactt gtacaaaaaa gtgagtatgc ttttgacttg gaacaagtcg
     1861 cacgtgtttg gaatcatggt tcggtgattc gcggctggct gatggaaatc atggaagatc
     1921 agttaaacga acatcccaat ttaagtaatt atcggggaat cgtttctgca tcaggtgaag
     1981 caaaatggac ggttgaaaca gcgctagaaa tggctgttcc agttccagta attgcaacta
     2041 gtttgttcat gaggaatctt tcacaggaag aagatagttt ttcagcaaaa gtagtttcag
     2101 cactaagaaa tgggtttggc ggacatgaaa tagttgaaaa gggtgagtaa aatgccagat
     2161 tatcatatag caatcgttaa ttccagcagt ttcggaagag tctttccaca ccacttggaa
     2221 agattaaagt cagttggaga cgttcatttc tttactgttg atcaagagat tccaggaaaa
     2281 gaactagcgg agttacttaa aggattcaat atcattattg caagtgttac gccatttttt
     2341 cgacgagatt tttttgaaaa caaagatgaa ctgctattga tttcccgaca tggtattggt
     2401 tacaacaata ttgatttaaa agcagcaaaa gaacaccaga caatcgtatc tatcatacct
     2461 gctttagttg aacgaaatgc tgtagcagaa aataatataa ctaatttgtt gtccatgctt
     2521 cgacgcacga cacaagcaca agcaagagtc aaggacgatc gatgggaaga tcgggcggag
     2581 ttcgtcggta ggacgctgtt taacaaaaca gttggtgtga ttggtgcagg aaataccgga
     2641 agcggagtaa tcgaaatttt gcgtaacggt tttcgctgtg atgttctagc ttatgatcct
     2701 tacaaatcag atttatacat cgaaagcttt ggtgcaaaaa aagtagaact tcaagaatta
     2761 ctggaacaat cagatatcat ttgtttatgt gcgaatttga cagaagagaa ctatcacatg
     2821 atttctgata aagaagtcga tcagatgaaa gaaaatgttt atctatctaa tagtgcaaga
     2881 ggcgctctag ttgatgaaga agcgatgatt aatggtctga aaagcggaaa gatcgcagga
     2941 tatgccacag atgttttaga agaagaaccg ggacgtcaag atcatccgta tttagcgttt
     3001 gaacaagtag tgattacgcc acatatatcg gcctacacaa gggaatgttt agaagcaatg
     3061 ggtgaaaaat gtgtcagaga tgtcgaacag atcagtcagc accttcttcc tgaacgttct
     3121 gtccagacaa agagcaacta tataaaagga tgatgaagaa aatggtgaac ggtttaaaag
     3181 tgaaaacagg ccctcaattt tatttatatg aagaaggtgg tatttcaaaa gtttctgatc
     3241 tcttgaaatc atacggcgcg aaaagagtac tggtcaccca cggaacggta tcatgggaaa
     3301 aggcgctacc taaattagta tttttgaatg acgaaacgat tcaatttttt tatcatcgtt
     3361 attctggaga atgtagttat gctgaagcca gacgaatagc tacaatcatc aaaaaaatga
     3421 aatcgatttc gtagttggcg ttggcggagg aaaactgacc gatctagtgg gttatagtgc
     3481 caatcttgca agaatcgatt tcggagtcat ccctacttta gctagtaatt gtgctccatg
     3541 gacacctctt gctgtcatgt ataaggagaa tggacaagca gaagggaaaa cagaacactt
     3601 ttacagacaa gctgcttttt ttataacaga tccccaatta gtgattgatt cccctgttcc
     3661 atatttcatt gccgggttag cagatacact agcaaaatgg tatgaatctg agctcatctt
     3721 agaacaagat tttttgcaag atgaatcatt tttgaagcta gcacaagata ctgcgaagat
     3781 ctgcaaagaa gagattttga atcattctgc taaagcaatc caagatatgg cacaaagaaa
     3841 actgacatcg gagttcaaac agctttcaga gattatcttt gctgttgctg gtttagttgg
     3901 aggattagga gataaatacg caagaaatgt ggcagcacat gctatgcatg acgctatcag
     3961 taaatatatt ccggaaagcc atcgttttct acatggagaa aaagtagcgt atgggatttt
     4021 ctatcaactg gcactagaag gtcgctggga aaaaatagac gagttatatc ctttgtatcg
     4081 ggaattagaa ctgccggttt ctctagaaca aatgggatta tttccgaaag ataaacagat
     4141 aatagataaa atggctgaat ttattgattc gaaagagaaa gtgcatttga ttcctataga
     4201 ggtaacacag aaatcactga tacagtcaat gatggaactg gaaggttata tcagaaagat
     4261 ctgaaggagg aggacacatg gaaacattaa gtgtagtaca aagtttgttg attgctttat
     4321 gggtagccgc ggttatgtcc cgctggttag gtggaggagc cacgttgacc ttacgttttt
     4381 ctcctttgat gactggatta gtcgtcgggt tgatcatggg ggaagttgca caagcgatga
     4441 ttgttacagc cgctttgcaa atgatttata tgggggtttt ttctccagga ggatcaatgc
     4501 cagctgaacc atctattgca gcagctatcg cagtaccagt cgcattatta ggggatctgt
     4561 cgccagaagc agcgattgcc gttgcggtgc cagtcggatt gctcggaagt tacttgtatc
     4621 agtttcgttt tttcatcaat acttttttag gtaaatatac agacaaagct gtagaagatt
     4681 tgaatacaaa agctatggcc cgatcaatta ttctttatcc aacggttgcc tcatttttat
     4741 tattcgttcc gcttgttttc attgctttat atttaggggc gccattgatc gctgatgtaa
     4801 tcaaagcttt agagggaaca gtcgttatcc acattcttga agtagtaggt ggagggttag
     4861 cggcaatcgg tatcgcgaca actgtatacg ttatcggtcg aaaagattat ctggtctttt
     4921 tcttccttgc ttattttatg agtgttgttt tgaaatcttt agatattacg atggttactt
     4981 atgccatttt tgggattatt atcgctttga tctttgtcca gtcgcaaaaa gggaaacaag
     5041 tacagcctgc cacaagcaat aacaattcag catcttttga tgacgaagat gactatgatg
     5101 acggatttta atttaggagg aatgggaaat ggctgaaata atggataatc aaacaggtgc
     5161 tcctgaagaa atcacgaaaa aagatgtaac aaaagcctat ctgagatggc attttgcaaa
     5221 cgaaattcct cactcttttg aacgttactt ggcgccatca ctactatacg gaatgatgcc
     5281 aatcttgaaa aaactttata aagatgaaga ctctttaaaa gcagcatata aacggcagtt
     5341 actgttcttc aacactcaat taagctgggg tggcggtgtg attactggcc taatgtcttc
     5401 tatggaacag caaagagcag aagaagagta tgaagaaaaa gagatcttga tgcaggatga
     5461 cttgatgtat aacacgaaag cgggattgat gggggcacta gcaggaatcg gagatgcaat
     5521 cgattcagga accgtccaat atatttttat cgcaatcgcg gtaccttggg cacaacaggg
     5581 aagtccttta ggcgcacttt ttccttttat ctgctttggg ttgtatcagg ttttactagg
     5641 ggtatttttc gcaagacaag ctttcactat gggccgtaat gctacaggcc taaagcaaaa
     5701 cactggtgtt cagacagcga ttgaaatgct atcagtttta gggttgttta tgatgggggt
     5761 gcttgcggga aactatgtaa aagtagaatc ttcattgaaa tttgcgatat ccggacgtga
     5821 gtttgttatc caggaaattc ttgatcaaat cgtgccaggg atattacctt tagctgtcgt
     5881 aatgggtgtg tacttgttct atacgaaaaa aggattgaaa gtcacacaag cattattatg
     5941 gctgactgga atattaattg tgctagcagg aatcggtatt ttataaaaag aagggaacgt
     6001 gaataagatg ggtgtagtca atttagcgcg tgtggatgag cgtttgatcc atgggcaagt
     6061 aatggtaacg ttatctcaaa aaagtggagt gaattccatt tttgttgtag acgaagttgt
     6121 agcaaaagat aaatttatga gagatcttta caaaaatgca ggaagccgta caggacaaaa
     6181 aacaattgtc attacaccag agaaagctaa attctattgg gatgaatatc aatttaaaga
     6241 atacaattgt attttgatcg caaaaacagt ccaaacgatt tacgacttag taaaacacgg
     6301 aatccccatg gaagaattga atattggagg gattgctcaa aaaaatcctg atacagatct
     6361 tttagtgacg aaatctgttt atttaaataa atcagatgct gaaaaattga aagaaatgaa
     6421 tgaacaatat ggcgtaaaga atatttattt tcaagccact ccatctgcac ctaaaacagg
     6481 attaaaagat gtattgaaca agtttgattt gtcataaaca aatgcttaga aaaaagataa
     6541 agagggcgaa aaaatgaaga agaacgagct gtttcgtgat tgggaatttc gttatcgcta
     6601 tatttaccgt aagcgccgta caaaaaagag taaacagcgt tttctttcag cattagtatc
     6661 agatatttac agtatgagaa cagatgtgac agtcattgct tacgatacgc tagcctaccg
     6721 atcaaaaaat atttatgttg gagatatcga aaaagcagag aaagttatct gtacttatta
     6781 cgatacaccc gttcatgcat taggaagtta tttcatgttt gattggaaag accaaaggaa
     6841 aaaaacaatc tactctatat tattgtcctt cattttgttg ttcagtttag gttggtgggg
     6901 aatgatgata tataacaaaa atcctcatca cgtattcgat ctgctatccg tataaacagg
     6961 tatcactgta ctagcatttg ggagctactt tttcttattg ggaaaagcag ctcgcggatg
     7021 gcctagcaga caaactttta ttcgaaatac ttcatccata ttaacgatgc tagaaatgat
     7081 ccgtacgatt gatgatccga gtgtagcgta tgcgtttgtt gatgaaggct gttatggaga
     7141 aaagggctta gacagcgtga gatcgggtat gaaaaaagag gctattcttt tttatctaga
     7201 ctctgttggg gcagatacac cgctacagtt ttcgggaaat tatttttcaa ataaagaaca
     7261 gtggctgaaa caagttgata agttgaaaga aaaaaacgtc aattatattt ttagtgcaag
     7321 aaaaaagcaa gcgcagttct tctatttgac caaaacagat ttacgaggga aaacatttaa
     7381 ttggcaaaat gccaatcaga ttattgctct ttttcgataa gtaaaaagtg caaaacaagg
     7441 agtgagaaga ctaatgttgg aaatcatgat cgttacacat ggtaagttga gcgaaggact
     7501 gaaagatgcg gcggaagtaa ttgtcgggac atcgagccat attgaaacaa tgtgcttaga
     7561 gtctggggag gacatccaaa cattcggaga gaaggtcaaa gaaaaattaa ctatgatcaa
     7621 cacagcagaa cccatgctag tatttgttga tctaattagc gcaagtccgt acaatcaagc
     7681 gctcttggca gtcaatgaac tgactgaaga aaagaaagca gccacttatg tcataggggg
     7741 cgtgaatctg ccaatgttgt tagaagcaat caatcatcag atgcttcaca caccgataga
     7801 agaagcggtg gaacaaattg tcaaacaagg aaaagataat ttaactgttt ggcatgcttc
     7861 agcggaagaa aaaaaattag atgaggacga tttttagaag gaaggctgaa aagatgaagt
     7921 ggggatttag atggtatggt gaaaaagggg attcgatccc attaaaatat gttcgccaaa
     7981 taccgggaat aaatgggata attggaactt tgctgactaa aatgcctgga gatgtttggg
     8041 agatttcaga aattgaggag ttgaaagcat ctgttgagag ccaaggactg gaactgcttg
     8101 gaattgaaag tgtagcgatc cacgatgcaa tcaaggcagg gacagatgaa cgagattact
     8161 atattgataa ctatatacga acaatcaaaa acttgtctcg atgtggggtt cgattgattt
     8221 gttatagctt caaaccaatt tttggatggg cgaaaactga tttgtttaat gaaaacaacg
     8281 atggaagcta ttctctttta tacgatcaga aaatcgtcga tcagatggag ccaggagaaa
     8341 tgtatcaatt gatacacagc cagtcaaagg gattcaaatt gccgggttgg gaagaagaac
     8401 ggttgaaaaa attcgctcag ctggaaaaac tgtatgaagg aatgaccgaa gaagaattat
     8461 ttgaaaatct gacttatttt ttagaaaaag tgattccagt gtgcgaaaaa gcagatgtaa
     8521 aaatggctat ccatccagat gatccgccat gggaactttt tggttttcct agagtgacga
     8581 aaaatcttgg cgacttgaag aaaattctaa atgcagtaga ttctccatat aatggactaa
     8641 cgctgtgcac aggatcttta ggggcaaatc cggaaaacga tttagttgaa atcatccatg
     8701 aggtaggttc gcgcatccat tttgtccact tcagaaacgt ggagttttta ggagaaagaa
     8761 agttcaaaga aacggcacat ccctctacag aaggctccct tgatatgtat catattatga
     8821 aagcgttggt gcaagaaggg ttcgacggtc ctatacgccc tgatcatggt agaaccgtgt
     8881 ggggtgaagt ggctatgcct ggatatggcc tgtatgatcg tgcaatggga ctcacttaca
     8941 tgcaaggtct atatgaggcg ataagtaagg aaaaaccact gggagagcat tatgaataaa
     9001 atcaagctct tcatctttga tatggatggc cttttattag aaactggacg tttagcttat
     9061 cgtgcttatg taaaatcagc acaaaagtat gattatgaaa tgcgtaagga agtctattat
     9121 ttactaactg gccaaacaga aatggcgata agagaacaaa tgggcatttt gtacggagaa
     9181 gatgttccat atatcaaatg gcgagaagca atcaatcaat ataaagaaaa aatcgtgaaa
     9241 gaagataaga gagtatatac gaaaaaagga gcagaagaaa ttttatcttt tgcaaaagaa
     9301 agaggaatcc atacaatcgt ggcttcgtct aatacacggg taaaagtaga aatgtattta
     9361 aaaatggaaa atctatatgc cttgtttgat caaatcattt caggggatga tgtaaaaaaa
     9421 ggaaagccgg aaccagagat ctttttgaaa gcttgttcaa aaatgaatat tcctccaagt
     9481 gaagcacttg tatttgaaga ctcgattgca gggatcgaag cagcaaggcg agcgggcatt
     9541 ttgtcttttc tcatcaaaga tcatttgata gggttgcccg atcataatgg gaagcataag
     9601 ttgaaagtag atgtaagttt atatgaatcc aataaagcag actatgaatt ttatgacctg
     9661 catcaagcaa aacgattttt agaaagtaaa gggttgtatc tctaataaag aggactattt
     9721 tctagtcctc tttatttatt gtcctgcacg actgagggaa cagatgaaaa agaagtaatt
     9781 ttttattttg aagtatctac cttatttgta gtgaacaatt tagaacaaac acaaaacgca
     9841 tctactgt
//
LOCUS       DACIRY010000102         9771 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.104, whole genome shotgun sequence.
ACCESSION   DACIRY010000102 DACIRY010000000
VERSION     DACIRY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.104"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(13..996)
                     /locus_tag="IYE22_002102"
     CDS             complement(13..996)
                     /locus_tag="IYE22_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ9507297.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="IYE22_002103"
     CDS             complement(1001..1648)
                     /locus_tag="IYE22_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9507298.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="IYE22_002104"
     CDS             complement(1733..2365)
                     /locus_tag="IYE22_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9507299.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IYE22_002105"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IYE22_002105"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ9507300.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="IYE22_002106"
     CDS             complement(2929..3666)
                     /locus_tag="IYE22_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ9507301.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="IYE22_002107"
     CDS             complement(3663..4652)
                     /locus_tag="IYE22_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ9507302.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="IYE22_002108"
     CDS             4910..5452
                     /locus_tag="IYE22_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9507303.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="IYE22_002109"
     CDS             5469..6554
                     /locus_tag="IYE22_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507304.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="IYE22_002110"
     CDS             6566..7372
                     /locus_tag="IYE22_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9507305.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="IYE22_002111"
     CDS             7373..8200
                     /locus_tag="IYE22_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9507306.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="IYE22_002112"
     CDS             8197..9270
                     /locus_tag="IYE22_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9507307.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         2971 a         1788 c         1989 g         3023 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg a
//
LOCUS       DACIRY010000103         9735 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.105, whole genome shotgun sequence.
ACCESSION   DACIRY010000103 DACIRY010000000
VERSION     DACIRY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.105"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(17..520)
                     /locus_tag="IYE22_002113"
     CDS             complement(17..520)
                     /locus_tag="IYE22_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9507308.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(643..1407)
                     /locus_tag="IYE22_002114"
     CDS             complement(643..1407)
                     /locus_tag="IYE22_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ9507309.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1515..1790
                     /locus_tag="IYE22_002115"
     CDS             1515..1790
                     /locus_tag="IYE22_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ9507310.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2228..3178
                     /gene="yidC"
                     /locus_tag="IYE22_002116"
     CDS             2228..3178
                     /gene="yidC"
                     /locus_tag="IYE22_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ9507311.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYHENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(3357..4331)
                     /gene="lacD"
                     /locus_tag="IYE22_002117"
     CDS             complement(3357..4331)
                     /gene="lacD"
                     /locus_tag="IYE22_002117"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ9507312.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(4328..4786)
                     /locus_tag="IYE22_002118"
     CDS             complement(4328..4786)
                     /locus_tag="IYE22_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9507313.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(4783..6246)
                     /locus_tag="IYE22_002119"
     CDS             complement(4783..6246)
                     /locus_tag="IYE22_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9507314.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(6228..7178)
                     /gene="pfkB"
                     /locus_tag="IYE22_002120"
     CDS             complement(6228..7178)
                     /gene="pfkB"
                     /locus_tag="IYE22_002120"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ9507315.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            7321..8069
                     /locus_tag="IYE22_002121"
                     /pseudo
     CDS             7321..8069
                     /locus_tag="IYE22_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            8335..>9735
                     /locus_tag="IYE22_002122"
     CDS             8335..>9735
                     /locus_tag="IYE22_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ9507316.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSI"
BASE COUNT         3040 a         2039 c         1665 g         2991 t
ORIGIN      
        1 ttattggttt tacctgttaa atcgaggatt ctttcccaaa gtaacgttgc catctcttac
       61 gcatacttgc agacatgggc tgagccgctt ctttgactgc gcagtatttg tccacgaatg
      121 tcacaatata tccttcttta tacttaggcg gtgcgatcgt tgcaccccac atgtgcttca
      181 aaatgatatc tcgctcaaga tccgacaaat cggtgatttt ttctgcattc tttacagcaa
      241 ttcttggatg gatatacgca tgtgtcccct cgtcaaattt cgtcgtacgc caatcatagt
      301 agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt
      361 ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa
      421 catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga tcttgtacat
      481 aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat ctgccacctt
      541 tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc ggattattct
      601 agtcgctcta gataaaatag ctaaagctta atagttcctt aatcataaag aaaaatgata
      661 aagaacgatc cctgctgcga ctccgacatt caatgattcc gcatttcctt tcattgggat
      721 atatacgttc tgatcagtca aagacaagat ttcctgactt actccctgac cttcgtttcc
      781 catgattata gcgaaatcct ctgttttttc tactttgtct aatgcatgtg cttcttcatt
      841 taattctgta ccgtacaccg taaacccttt gcctttcatt acagggatca cttctgccaa
      901 atctttttgt aacacaggca aatgataatt gcttccctgc atgcttcgca gcacctttgt
      961 actataaata tctgccgttc ctttacctaa taaaacacca gccataccaa acgcatctgc
     1021 cgtacggatc atcgttccca catttccagg atcctgtaca ttatctagta aaagccagcc
     1081 tcctgaaaaa tctggcgtat cttcagatgt taacgtcatc accgctaaga tcccttgcgg
     1141 tgtaggtaga tctgacatag cggtcatgac ctcatctgaa actagaaaag tagaaatctc
     1201 tttgtttaca gccagccagt ctccccattc tttcatacct ctctgtgtca caaaaagttc
     1261 ttttatttct gcatcagacg acgcagcttc ttcgatcaga tggaaccctt ccaaaagata
     1321 ttctgccttt tcttctctgt atttcttttt atgtagcttt tttaattctt tgatttttgg
     1381 attttttgct gattggatct ctttcacatt ccttcacctc acctgattat atcatggaat
     1441 aggtgcttga gcttgtttca tcctaaaaaa atcggtatca ttgatttata aaacaggaaa
     1501 gaaaggtgac gaacatgcgc aaagttaaaa tgaatgtcca aggacgtgtc cagggcgtgg
     1561 gatttcgcta tatgaccaag atggttgctg atcagttagg cgtgaccgga acggtaaaaa
     1621 acgaagacga cggctcagtc acaatcgaag caatcggaaa tgacgacatc atccaaaaat
     1681 ttatcgaaga agtcaagaag tctccgagcc caagtggacg agtgcagtat gtggatgtcc
     1741 aagaagatcc gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa
     1801 aggtcgctta gttttgagta ttaaggaaaa ttttgtgaaa atggctttcc acatttttgc
     1861 ataaatttga cttaataccg agaaactgac ttttgaacac cgtcgatcag gttgtttttg
     1921 aggcgctttg cctcaagtat taaagatagg tagtaaaaaa gctgatctat aacttgcaat
     1981 aaatttgctt aacccatatt ttccagacag aacaaaaaag tttttagttt tttatataag
     2041 aaagaaacac ttggaatcac tcttttcttt ccaagtattt ctttcttgat tttttgtctt
     2101 ttttcaaaat ttttcgctac gaaaaagaaa gaatatttat atgaaatttc tatgaagttg
     2161 attgaattcc atgcgctttt ttagtatagt taagtttgtg taaaaattat agaagaggaa
     2221 gtattctatg aataaatgga aaaactggtt attgggatcc ggacttgtca gtatcctgct
     2281 ctttttatct ggctgtgtgc ggatggattc aaacggaaac cctgacactt caggaatcgt
     2341 ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg agaatttcaa
     2401 ttggtcttac ggctgggcag ttattgcaat gactgtgatc gttcgtatca tcatcttgcc
     2461 actaggtatc agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc aagcgttaaa
     2521 accacaagtt gaagcagcac aacaaaagct aaaagctgca acaacacgtg aagaacaaat
     2581 ggcagcacaa gcagaaatgc agcaagtata tcacgaaaac ggattgagta tgaccggcgg
     2641 aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt attttacggc
     2701 tcgctacaca gaaggtatcc gtgaatcatc tttttacggc atcgatctag gctcaccaag
     2761 tatggtgctt gtggccattg caggggtcgc ttatctgctt caaggatata tttcaacaat
     2821 cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct ctcctgctat
     2881 gatcgtcttc atgtcgatca gtgcgccagc cggcgttaca ctttactggg tagtcggtgg
     2941 tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg atgaaaccac gcttgaaagc
     3001 acaagtggca gaagagttga aacaaaaccc gccaaaacaa gtcgtcactc caagaaaaga
     3061 cgtgacagaa tcagcaaaac cagctgacaa caaacaagca acaaaacaaa tcaaacaacc
     3121 aaaaaacagt acaaataaca aaggccgcaa cgcaggaaag cagcagaagc ggaagtagat
     3181 agtaaataag agtgtaataa aaattcatta acagttttag caattaaaaa tatcgaccaa
     3241 gatttttgaa aaagaaatct agtcgatatt tttatttaag tgaatgtata atttgaatca
     3301 tcgattgatc gctatctaat tgcagcagca tctttttatg aagaaacaac tttttcttat
     3361 ctatcaacgt agacaggtgt tgctgtttct tttaatagtg tacttaattc cttaatattt
     3421 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
     3481 gccactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
     3541 tgttttgcaa aacgtaatgt atcttgaaat aacgtagcat tgactcccgc acttaaaaaa
     3601 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
     3661 ctataacaag tctcttgccc atatccttcc acaaatctca tattcactgg cacttctact
     3721 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aataacttta
     3781 tggggtttta cttttgcata ggcaagagag gacggatcgc caatagttgc atcataagaa
     3841 aggatctcta aaaagaaagg tatgccttct gcatcgcatt ctgaaccaat ccgttctata
     3901 actatttgtt tttgcacatt aatctctgct ggttcatcca cgtcatagta caataaaaat
     3961 ttacaagcat ccgcaccagc ttcttttaac cttttcaccg accaaatatt caataaatca
     4021 ggtaatcttc ccggcatttt cgtatcatag ccggtttttt cataagctag cagcaagcca
     4081 cattctttct ctctaagcgc tgctgcaggc aggccgcatt ctggatcaag taaaatagct
     4141 gaggcatagg gagtcaggta ttgagaaacc atctgtttaa attggatgat gtcttctgat
     4201 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgctggtc gattgctaac
     4261 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgctt ttctatactc
     4321 atcttcttca tagatcttca cctatttctt tagttaatat tgtggctatt tctgtttttg
     4381 attttgcttt tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
     4441 acaataattt caaatgagtc gttcctgctt ctgaatctgg aataaataac gcaaaaataa
     4501 aatcaactga ctgtctatct aaactgttcc attcaattcc tttgttcagt gtgacgataa
     4561 tcatactagg ttcgttgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
     4621 ttcctgtagt accttctttt tctctttcta gaagttttcg atatacttct ttttcgtcat
     4681 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgactat
     4741 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata agcctcctgc
     4801 tcttccttct tcgataactg tacgatttgc tgctttatta tccatcttct tgtctttatc
     4861 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
     4921 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
     4981 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
     5041 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccctccgt
     5101 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
     5161 atatttacca aacaataaag ttgcaaaacc agtaatcaac ggtggtgcaa tgcaagctac
     5221 tgacacgcca gccataaaag tcgtgtttcc ttgtgccaaa agagctgtac ctgttacata
     5281 tgccgcctta ttaatcggtc ctcccatatc aaacgcacac atgcaaccaa caatcacgcc
     5341 aagaacaagt ggattcgaat gttcaaatcc agctaaaaag ctcatcattc cttcatttat
     5401 agccttcatt ggacccgcca gcaaagacat cacgatgccg ataaacacta caccaataac
     5461 tggatataag aagattgcct tcaagccatc tagttgttta ggtaaaattg tcagtagctt
     5521 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaataccta ctaaaaatcc
     5581 agtaccgcca ctcatggcca cgattccacc aacaaatcca acgactaacc ctggacgttt
     5641 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcatcccca ttgctgcatc
     5701 gccaaccgtt tttaacatcg ccgctgtagc attgtattgt gcatggtctg gatcaaaaga
     5761 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
     5821 caacatatga gagacgccat tcattaaatt tttatagatg ctgcgaccaa aattagcacc
     5881 acttatcgtt tctgattcta aagtatcaac agcttcatta tctgctaaga tggtcccttt
     5941 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
     6001 atcaatgact cgttttcccg caaatctttc tgaatggaca tctttgtctg cggcaataat
     6061 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
     6121 atgggtctcc actttgatcg tgatgccttt ttttctagct gcttgctcta atgcctcttg
     6181 cgccatgtag gtgtgtgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
     6241 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
     6301 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
     6361 aacgtttcat caagcggtcg tcgattcatg tatcctacta aaaaagtgcc taacatggca
     6421 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
     6481 tctttcgtaa aaagaatcgc tcctttctca ccaagggata acaaaacatt ttctgcacct
     6541 gactctacca gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
     6601 aaccaagaag aaagttcttc ctcattcggt ttcagcaaaa atggtcgata gggcaaacaa
     6661 tccatgacct cttgatcact gctatcaata atcaaatgaa tctccttttc ctgacaaatc
     6721 cgactgattt ccactaaaac cttcggagac attcctcttg gtaaactccc agaaacacaa
     6781 agataatcct ctgcttgtaa tgctcttatt tgatttaaaa aattatggac agctgcctgc
     6841 ggtatttcag gtccttgatt cactaatttg tattcttctc tcgtttgatt gacttgtgta
     6901 aatacattga tacgcgtcat accaggcact tcaataaact ctgtttggat ttgtttcttc
     6961 tgtaaatagt cttctatata ttttcctgtg aaaccagcag aaaaacctaa tgccgtactt
     7021 gggaaatcta gcatctttaa tactagtgac acatttactc cttttccgtt tgcctgaatg
     7081 tcatcctcct ttgtacggtt taccataaaa ggatgcatct cctctgtttc aataaataga
     7141 tcaattgcca aattcatcgt gcatgtgtag atagacataa ccaatgacct ccttgaatct
     7201 attatgaaat acgcgaaaga gaaaatgttt tcttaatcgt tccaacacaa agaaaacctt
     7261 tacatttata aacacatcgt gctatttttt aactaacttt tagtgaaaga aggaaagaat
     7321 atggatcaca aattaagtga aacagaacag tacctctgga actttatcga acaccacatc
     7381 ctagagatcc ctaactactc catcgtgaaa ctaagtgaac aagcaaatgt ctcaacagcc
     7441 accattgtgc ggacaatgaa gaaaaaaggc tatgaaggat ttacttcgtt taagcaccat
     7501 ctaaaagaag gagaaaacaa gaacatcaat ttttcttttt agataaagta gacaaaggta
     7561 tccgtcgagc cattctaaaa aatgaacaag aagtggtacg tacgatcaat atgttggaaa
     7621 tcgggaacat tgaagatgca atacaaagaa tcaaatttac tgaccgggtt tttgtctttg
     7681 cccgaggatt ttcagaaatg attggtcaag agatgctcgt aaaacttcaa ttaacaggaa
     7741 aaaattgtca aatgcatact gatccagaga ttatcaaaag catcagtcgg aaactaacta
     7801 agaaagacag cgttattttt gtttctttga acggcgagac aaaagaactc gtcaccgccg
     7861 caaagaactg ctatgatgca gagattggtt cgattctagt tacagccaat cattcctcta
     7921 cactcaaaga ttattgcgag ataaatctcg taggtttcaa atcagaaggt tcttatttcc
     7981 ctgattatga agtacgttca cgcttgcctc tccagatcat cgcgagaatc ctgcttgacg
     8041 cctatgtatt gcgtatgagg gagaattgag aagcagttta ttatggttgg ttacagttta
     8101 aaacatcaca ttttttattc tttatattgt atggactcac gtaaactttt tatgtataaa
     8161 gttattcaca atctataaag ttattttaag taacaaaaaa taattgaaaa tacttaaaat
     8221 caaaaaatat ctttttaatc aataaaaaaa ttgacagttt tattaataat aagctattct
     8281 gttatagaag aaaaaattgt ttgttattat tagcaacaaa aaaaggagtg catcatgaaa
     8341 aagttatctc aaaaacgttt agctcaaatc attattgatt ctagaaaatc tctgaaaatg
     8401 acacaacaac aacttgcagt agctactggt atcaaccgtg ccttaattag tcgagtagaa
     8461 tctctcgatt ttatcccgtc aatacaacaa ttagaatcat tagcagaagt tctaaatttt
     8521 gaaccaacag atctatttat aaatgaatca aataaatcaa acagaacaaa acatcagtcg
     8581 aagaatattg ctgtagccgg tactggttac gtaggtcttt ccattgctac tcttcttgcc
     8641 cagcacaatc aggtaacagc agtagacatt cttcctgaaa aagtaaaaca aattaacaat
     8701 aagaaatctc caatacaaga cgattacatt gaaaaatatc tagctgaaaa aaatcttaat
     8761 ttaattgcaa cgactgatgg tgcagccgcc tatagagaag ccgattttgt tgtcattgct
     8821 gctccaacaa attatgatag taaacaaaat tattttgata cttctgctgt agagtcagta
     8881 atcaaattgg tgttagaagt caatccaaag gcaataatgg tcatcaagtc gacggtaccc
     8941 gtaggctata ccgaaagtat tcgtaaaaaa tacaatacta aaaatattct attcagccca
     9001 gaatttctca gagaatctaa ggcattatat gacaatctat atccttcgcg aattattgtc
     9061 ggcacggacc aaactgacaa aactttacga gaagcctctc acacttttgc agatttactc
     9121 caagaaggag caatcaaaaa agatatcgaa actcttttca tgggattcac agaagctgaa
     9181 gcagttaaat tattcgctaa tacttacctt gcacttcgag tatcttactt caatgagctg
     9241 gatacttatg cagaaatgaa aggactaaat acacaagaaa tcattcacgg ggtttgctta
     9301 gatccaagaa ttggcacaca ttataataat ccatcttttg gatatggagg ctattgttta
     9361 ccgaaagata caaaacaact tctcactaac tataacgatg ttcctcagaa tatgataagt
     9421 gcaattgtcg atagtaatcg tacaagaaaa gactttatag cagatcgagt gttagaactt
     9481 gctggcacgt atgaggctaa cagttcgtgg aatgcagaaa aagaaaaaga ggtagtcata
     9541 ggtgtatacc gtcttacaat gaagagtaat tcagacaact tccgtcagtc atctattcaa
     9601 ggagttatga agcgaattaa agcaaagggg gcaactattg taatttatga accaacactt
     9661 gaaaataaca ctacattttt tggttcaaaa gtaataaata atttagatga ttttaaaaat
     9721 atcagtcaca gtatt
//
LOCUS       DACIRY010000104         9734 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.106, whole genome shotgun sequence.
ACCESSION   DACIRY010000104 DACIRY010000000
VERSION     DACIRY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.106"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            264..1457
                     /locus_tag="IYE22_002123"
     CDS             264..1457
                     /locus_tag="IYE22_002123"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ9507317.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IYE22_002124"
     CDS             1587..3059
                     /locus_tag="IYE22_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9507318.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IYE22_002125"
     CDS             3142..3930
                     /locus_tag="IYE22_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ9507319.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="IYE22_002126"
     CDS             complement(3997..4665)
                     /locus_tag="IYE22_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ9507320.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="IYE22_002127"
     CDS             4780..6492
                     /locus_tag="IYE22_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507321.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="IYE22_002128"
     CDS             6495..8267
                     /locus_tag="IYE22_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507322.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            8393..9732
                     /locus_tag="IYE22_002129"
     CDS             join(8393..8834,8834..9732)
                     /locus_tag="IYE22_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ9507323.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         3137 a         1573 c         2072 g         2952 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg tttttataag gggcagaaat
     8341 gatgtgcacc ccaaaagtta gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa
     8401 atatacattt gtttttaaga aaaaagtagt ttcagactat ttaaataacg aaggcggcta
     8461 caaatatctt gcacataaat atcaaataaa tcgtacactg gttagacatt gggtaaggat
     8521 ttataactat catggttggg aaggcttggt tggaggtggc aaaagctaca ctacaaaatt
     8581 taaacttgat gttatagaat atatggaaac aaatggtctt tctatccaag aaactgctaa
     8641 aaaatttaat atcggttcaa atagaactct aagtaaatgg atagagcaat atgaagaagg
     8701 cggtgcttct tcacttgaga gccaaaaaag gggcagaaaa attagtatga attccaagct
     8761 aaacattcct aaaaaactta aagatgagtc tcttgaagaa gaagttattc gtttaagagc
     8821 agagaacgca tatttaaaaa agttagaaac cttgattcaa gaacaagatt tgagcaagaa
     8881 gaaatcaagg ttaaaacaat ccttcagctc aaaaaagaat ttaaattgaa tctattactt
     8941 tcaattgcac aattagcgaa gtcaacttat tattactggg taaaaaaatt agataagcca
     9001 gataaatata gcaagataaa gcaagaaatt acagcgattg taaaagaatc aagaaactct
     9061 tatggttatc gtagagtcac tttagcgtta aagatgaaag gatatacaat caatcataaa
     9121 acagttagaa aattaatgtc ccaaatggga cttacctgtc aaatccgaat aaagcgatat
     9181 aaatcttata aaggaacagt aggaaaaatt gccaagaatg tgttaaaacg aaatttttca
     9241 gtagatacac ccaataaaaa atgggtgaca gacgtcactg aattcaagat aaaaggaaga
     9301 aaaatctatc tatctcctat acttgattta tttaacggag aaataattag ttatagtata
     9361 tcaactagtc caacatataa actgattgaa gaaatgcttc aacaagcaat taaaaaaaag
     9421 ggaactgagg gttcactgat tctacattca gatcaaggat ggcagtatca gatgccacaa
     9481 tatcaaaaaa aattaaaaga gaataacatt attcaaagca tgtctagaaa aggaaattgc
     9541 ttagataatt ctgtaataga aaattttttt ggtgtgctta aatcagagtt tttttatcga
     9601 gaaaaattcc gatcaattga gatatttcaa agtaaattaa atgagtatat taggtggtat
     9661 aataacaaaa ggataaaact aaagttaaat ggactatctc cagtagaata tcggaaacag
     9721 tccattaaat agtt
//
LOCUS       DACIRY010000105         9433 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.107, whole genome shotgun sequence.
ACCESSION   DACIRY010000105 DACIRY010000000
VERSION     DACIRY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.107"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            7..729
                     /locus_tag="IYE22_002130"
     CDS             7..729
                     /locus_tag="IYE22_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ9507324.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            787..2826
                     /locus_tag="IYE22_002131"
     CDS             787..2826
                     /locus_tag="IYE22_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ9507325.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2935..4086)
                     /locus_tag="IYE22_002132"
     CDS             complement(2935..4086)
                     /locus_tag="IYE22_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ9507326.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4240..4770)
                     /locus_tag="IYE22_002133"
     CDS             complement(4240..4770)
                     /locus_tag="IYE22_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507327.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4869..5219)
                     /locus_tag="IYE22_002134"
     CDS             complement(4869..5219)
                     /locus_tag="IYE22_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ9507328.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5376..6905)
                     /gene="ahpF"
                     /locus_tag="IYE22_002135"
     CDS             complement(5376..6905)
                     /gene="ahpF"
                     /locus_tag="IYE22_002135"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ9507329.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6915..7478)
                     /gene="ahpC"
                     /locus_tag="IYE22_002136"
     CDS             complement(6915..7478)
                     /gene="ahpC"
                     /locus_tag="IYE22_002136"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ9507330.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7622..8284)
                     /locus_tag="IYE22_002137"
     CDS             complement(7622..8284)
                     /locus_tag="IYE22_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9507331.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8468..>9433
                     /locus_tag="IYE22_002138"
     CDS             8468..>9433
                     /locus_tag="IYE22_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ9507332.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKA"
BASE COUNT         3179 a         1790 c         1727 g         2737 t
ORIGIN      
        1 tctattttgt tactgcttac attagtagtt ggctattttt cgattgagtt tatccatgga
       61 ttttcgtctg caaaacagag ttcaactgtg aaaaaggtag agccgaaaag tatccctacc
      121 acactaaatg tggctttgat tggttcagat gctcgttcga aagacgaaaa cggtcgctcg
      181 gattcacttc tggttgcaca atacaaccag aaaacacaac aagcaaaact gatctctatc
      241 atgagagatt catatgtcga tattccaggt tacggaatga ataagataaa tgcagcttac
      301 tcttatgggg gagttgattt attaaatcaa acattaaagg aaaatttcaa atttgaagct
      361 ccctattatg caagtatcac atttcaagat tttatcgatt gcgttaatga actgtttcct
      421 ggtggagtca agatcgatgc agaaaaatct ctagatttag atggcgtata tataaacaaa
      481 ggagagcaag tgatggatgg taatacttta ctccagtatg ctcgatttcg ggaagacaaa
      541 gaaggagact tcgggaggat cagaaggcag cagcaagtga tcaaagctat ctctcagcag
      601 ctgaaagacg taacttcaat attcaagcta cccaaagcgg ttgggaaatt acttggtagt
      661 atccaaacga accttccaga aagtgtgctt ctggattgtg gtatggattt cctcaaagac
      721 atatgttaga ataaacaggt ataaatagtg aaataaagga atgacaagca agagaaggag
      781 gaaaaaatga aaagaagtga caagcacggc aaaaatcgaa caggcgctta tattgccggc
      841 gcagtgatct taatagcagc tgcgggtggc ggttattttt actaccagca ctatcaagaa
      901 acccaagcag tagaagctgg agaaaagaca gttgagcaat ttgtccaagc tttgaacaag
      961 ggagattata acaaagctgc agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta
     1021 tctgaaaaag agatcttaga taaataccag aatatatacg gtgctgctga tgtcaaagga
     1081 cttcagatat cgaatctaaa agtagataaa aaagacgatt ctacttacag tttttcatat
     1141 aaagcaaaga tgaatacctc attaggtgaa ttgaaagatc tttcttataa aggaacatta
     1201 gacagaaatg atggtcaaac cacgatcaac tggcaaccca acttggtttt tccagaaatg
     1261 gaaggaaatg acaaagtaag cctgaccaca caagaagcag caagagggaa tattatagat
     1321 cgaaatggtg aaccattagc aacaacaggc aaactaaaac aattaggagt cgttccaagt
     1381 aaacttggag atggaggcga aaaaacagcc aatatcaaag cgattgcttc ctccttcgac
     1441 ttaacagaag atgctatcaa tcaggcgatt tcacaaagct gggtacaacc cgattacttt
     1501 gtcccattga aaatcattga tggagcaacg ccagaacttc cagctggagc taccattcaa
     1561 gaagtagacg gcagatatta tcctttgggt gaagcagctg ctcagctgat tggttacgtg
     1621 ggagatatca cagcagaaga tattgataag aatccagaat taagcagtaa tggaaaaatc
     1681 ggtcgctcag gtttggagat ggcttttgat aaggatcttc gtgggacaac aggtgggaaa
     1741 ttaagcatta cagatgcaga cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa
     1801 aatggcaaag atatcaaatt gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt
     1861 ctaggaggaa aagctggatc aactgttgcg acaacgccaa aaaccggtga tcttcttgcg
     1921 cttgctagct ctccaagcta tgatccaaac aaaatgacaa acgggatctc acaagaagat
     1981 tacaaagctt atgaagaaaa tcctgaacat ccattcatca gccgatttgc gacaggttat
     2041 gctcctggat ctacgtttaa aataattaca gcagcaatcg gtctcgacaa cggcactatc
     2101 gatccaaatg aagtgttgac gatcaacggg cttaaatggc aaaaagacag ttcttgggga
     2161 tcttttcaaa caacgcgtgt cagtagtgat gtatcacaag tagacttaaa aactgctttg
     2221 atctattccg ataatatata tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt
     2281 cgtacaggct tagataaatt catttttggt gaagaccttg atttgccaat tagtatgaat
     2341 ccagcacaaa tttctaatga agatagcttt aactcagata tcttgctagc tgatactgga
     2401 tatggacagg gcgaacttct aattaatcct atccagcaag cagcaatgta ttctgttttt
     2461 accaacaatg gcacacttgt ctatcctaaa ttgattgcag ataaagagac aaaagataag
     2521 aagaatgtca tcagcgaaac agcagtacaa acgatcgtgc cagatctgag agaagttgtg
     2581 caagatgtaa atggtacagc acattctctt tctgctttag ggattccgtt ggcagcgaaa
     2641 actggtacag cggaaatcaa agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg
     2701 tttgctttca atcttgataa ccaaggttat atgatggtca gcatgttgga aaataaagaa
     2761 gatgatgatt cagcaaccaa acgagcatcg gaactattgc aatacctcaa ccaaaattat
     2821 caataaaaaa ttgcttttta agtacagtaa aaggactgtg gcgttatcgt cacagtcctt
     2881 ttttcaaata aacggttttc acgatgtctc tttcacaact tctttttgat ttactcacac
     2941 ttcataaact ttttttgtaa aatatttgag aacaagtgga gaaataagtg tacttaataa
     3001 aatcacgata actaaaggtg aatagtactg tggttcgatc aattgacttt gctgtccaat
     3061 ttgtaaaatg atcaaagcca tttctccacg cgaaatcata ccagcaccga ccatcaatga
     3121 actatgttga gaaaaaccgg caatctgagc accaaaataa cctccaagca atttggtcaa
     3181 aatcgctaca agcgtcaata tagaaataaa gagtagttgt tcactaaagt cagaaaaatc
     3241 aacttctaat cctacactga caaaaaatac tggtataaat actgcatatc ctaaagcctc
     3301 tacattgtgg tatacttctt ctttaacctt tgtctgactg acagctatcc cagcaaaaaa
     3361 agcaccaatg acagagctaa gcccaacaag atccgctacg taagacattc ctaaacaaat
     3421 aactagcgat acgatgataa tagaagaatt tgcgtacact ttttctgcaa atgaaagtag
     3481 gaaaggcgca atccatttga ctaataagaa aataaaaaca aaataaatta gctgttcaat
     3541 caaaacaagc ggtaaagaag cattgctaga agcttccccc gttagaaaag ataggctgaa
     3601 acttagaact aaaacgacta aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc
     3661 ttcttttgta ttcactacgt ttagttcttt taatacttct actgaaatac tgacagaagt
     3721 tgccgcaaga ataatcccaa agaaaatcgc ttcatttccc gaaacttgga atgctttgcc
     3781 tgtcaaccag ccaaaaacaa ccgggaagat gatcccaagc aaagcaacga acataccagg
     3841 tcggaaatat tttttcaaaa gagataaatc actttccaga ccagctaaaa acattaataa
     3901 gatcacacca atctccgaaa agtcatggac tagaatatca ggtttgaccc aattcaatcc
     3961 agcttggcct agcaaaacac caacaagtaa ttgtccaatc actgctggga tacctaatct
     4021 ccgcgagata tgtgagccaa tcgttgttga aatcaatatc aggcataaaa taccaataaa
     4081 ttccattctc atcattcctt tgtataaaat atagcccgaa cttagcataa catacattat
     4141 tgaaaaaata ggaaaatata ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt
     4201 gttggttttt atacggtcta ttttaataac taaaaatgat taacgtatag tcatttttct
     4261 tattctgatg aaattgataa tctcctggat gcccatgaaa atcaaaataa agccgaatat
     4321 ttggaataga agcatcaaac ttttgaatgg attgaacaat aagacgaatc cgccaatcaa
     4381 caacagaata ctactataaa cactccaact tttagctttt gtattcatgc tgaagaacaa
     4441 ctggaacaag ctgttgatga taacagctaa tccaagaaga atcggcagga tagaaacgat
     4501 tgcaggtgca aaagcataga cgatcagagc taagatcagg aaaaaaagac cactgaacag
     4561 accgatactc caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa
     4621 cccaagcaaa acaagatagg cggtgatcag ataaccgata aaatgaaaaa cagcagaagg
     4681 gtttagcaca atcgcaatcc cagaaataat ataaacagca gctcttaata aggcgtattt
     4741 ttgaatattt tgtttaatcg tctcaaacat ataagaaaac ctcctttgac ttattttaac
     4801 gtgaactagt cattttacca aattaaatgt tacaaaatga gcgaaggaga agaatatctc
     4861 tggaaaaatc aacttctgag gatcttttcc atttttttgc cttttgctag ctcatcgatc
     4921 attttatcta aataacgaat ttctttcatc aagttatcct cgatgtcttc tactcgtaca
     4981 ccacaaacaa cacctgtaat caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa
     5041 aattgttcga aagtcacttg tttttgtagt tgatattcta gttctgtttg ggaataaccc
     5101 gtcagccaac aaatgatttc atctacttcc tcttttatac gatcttttct ctccgctttt
     5161 gctacataca taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata
     5221 atcagtcacc tttctttttt tcaaattatt tactacttat agtatataag aaaaagagaa
     5281 gaaaaagcga ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt
     5341 ttttccgctc tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat
     5401 gctcctaaag ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta
     5461 tctgtacaat caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc
     5521 actatttcat ttcttggatt catttcgatc gttccatcta gccaatccgt attagggagt
     5581 aagccgacta gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga
     5641 tcgatatagg agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt
     5701 ttgataacag tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt
     5761 aattctggta agaattctag aacatataca tgttttgcta acccagccaa atcgattgct
     5821 gcttcaacac cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg
     5881 ccgtcacagt gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca
     5941 ttgatagaac gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata
     6001 ccgttagcta agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga
     6061 ttcatcataa tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt
     6121 ggaccttctg tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt
     6181 ccgccgaaag aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct
     6241 gcactactag ctgctggtcc accgccaatt actaatacat cataaggttc tttttcaaac
     6301 aactcttctt caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt
     6361 ccgctactaa attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca
     6421 tcgatttctt tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat
     6481 acagctaata tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca
     6541 taactgtcaa aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg
     6601 attttcggtg cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga
     6661 ccgaggggga taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa
     6721 ctaggtgtgc gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt
     6781 tccttcgtaa attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa
     6841 acaataggag attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct
     6901 aacatatcca ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca
     6961 ctttctttcc attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg
     7021 cgaatcttat caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata
     7081 gcttgaacga ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct
     7141 tcatccaaaa catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat
     7201 tcgattttac cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg
     7261 gtagagcaag aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag
     7321 tcttctaatt cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc
     7381 cagttcccca taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt
     7441 gcattaaatt cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt
     7501 atttatttat aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt
     7561 taataaagat tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat
     7621 gctactgctg gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca
     7681 tgtgtaagtg caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct
     7741 tgagtaaatc ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc
     7801 gtaaccagcg gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt
     7861 ttcttcctgc aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc
     7921 gttcgtcatt tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc
     7981 gtgtttgtgt gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc
     8041 ggacaaagcg agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt
     8101 gaggtatcgt atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag
     8161 tccggccgat agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta
     8221 agtcagcata gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt
     8281 tcatctttat tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa
     8341 acaatctggt atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca
     8401 tagcgatttt tattgtataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag
     8461 gggaaaaatg attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga
     8521 agtagaaata cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt
     8581 ctggatccat ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg
     8641 tttagcggat ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga
     8701 aataattgaa agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag
     8761 cagtggagag ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc
     8821 agattgttct gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt
     8881 agttactttt gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat
     8941 aggaccgatt gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat
     9001 tcttgctgcg ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc
     9061 tacagataat ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa
     9121 agaatggcaa aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga
     9181 aattgttgcg tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt
     9241 tagcaaaatg ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg
     9301 aaataatatt gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca
     9361 ttttcccaat ggacgtccga aattcgaaaa agctggggta ttcttgactg atcgtgcaac
     9421 ggtagataaa gca
//
LOCUS       DACIRY010000106         9400 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.108, whole genome shotgun sequence.
ACCESSION   DACIRY010000106 DACIRY010000000
VERSION     DACIRY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.108"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            157..351
                     /locus_tag="IYE22_002139"
     CDS             157..351
                     /locus_tag="IYE22_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507333.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            620..919
                     /locus_tag="IYE22_002140"
     CDS             620..919
                     /locus_tag="IYE22_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507334.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1016..1450)
                     /locus_tag="IYE22_002141"
     CDS             complement(1016..1450)
                     /locus_tag="IYE22_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507335.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1682..2392)
                     /locus_tag="IYE22_002142"
     CDS             complement(1682..2392)
                     /locus_tag="IYE22_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ9507336.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2382..3242)
                     /locus_tag="IYE22_002143"
     CDS             complement(2382..3242)
                     /locus_tag="IYE22_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ9507337.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3247..3885)
                     /locus_tag="IYE22_002144"
     CDS             complement(3247..3885)
                     /locus_tag="IYE22_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ9507338.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3900..4199)
                     /locus_tag="IYE22_002145"
     CDS             complement(3900..4199)
                     /locus_tag="IYE22_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9507339.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4242..5726)
                     /locus_tag="IYE22_002146"
     CDS             complement(4242..5726)
                     /locus_tag="IYE22_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ9507340.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5746..6207)
                     /locus_tag="IYE22_002147"
     CDS             complement(5746..6207)
                     /locus_tag="IYE22_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9507341.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6225..7292)
                     /gene="ulaG"
                     /locus_tag="IYE22_002148"
     CDS             complement(6225..7292)
                     /gene="ulaG"
                     /locus_tag="IYE22_002148"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ9507342.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7510..8280
                     /locus_tag="IYE22_002149"
     CDS             7510..8280
                     /locus_tag="IYE22_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9507343.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8553..9380)
                     /locus_tag="IYE22_002150"
     CDS             complement(8553..9380)
                     /locus_tag="IYE22_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9507344.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2915 a         1927 c         1479 g         3079 t
ORIGIN      
        1 gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac tttcaaattg
       61 attcttttta tttatcataa agatatagat acataatcat tttcacttac atatatttga
      121 atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga agtaaaaaca
      181 aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc tttacttggt
      241 tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa gaacgagcta
      301 gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta aatccaatgt
      361 atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta tagaattaga
      421 agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc atatgattat
      481 ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga atcaaatata
      541 cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat aatgtatcag
      601 tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac tacttaagca
      661 atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag tgacagagta
      721 tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa aaggaatata
      781 cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa
      841 tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt
      901 gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact aagtctatcc
      961 tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt atatgttatt
     1021 tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa ataccaccca
     1081 tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc tcagtatcat
     1141 ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa gcttcattca
     1201 aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata tcttgaataa
     1261 atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc ccttccccat
     1321 tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca gtgtcttttg
     1381 tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc ataagggaat
     1441 caatattcat ttttttcttc ctttcttata caattctccc tctaataata cagttctttg
     1501 actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc ttaaaattcc
     1561 tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa attttcttca
     1621 caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta ccctagcatc
     1681 tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta tctagtaaat
     1741 attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc atcagacaaa
     1801 tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc catgtgaacg
     1861 gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt ctttccttaa
     1921 acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact tcttctttcg
     1981 ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc gtgccataag
     2041 ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa
     2101 tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga tctgaagatg
     2161 gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc atttgatctg
     2221 cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct agctcacggt
     2281 ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc aatgccagat
     2341 ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac atcatagcca
     2401 gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct tcgaaagtct
     2461 tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag acgtttcaaa
     2521 tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc acgaaactgg
     2581 cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt gatataatcg
     2641 atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa attgcctagg
     2701 tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa aaattttgaa
     2761 atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc atatttagaa
     2821 gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt cattgatttc
     2881 tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag attatgcgcc
     2941 aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg atcagcagaa
     3001 ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac accggagctg
     3061 aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg tgctaaccgc
     3121 tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt tacaagggaa
     3181 aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc aatttgagcc
     3241 atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg
     3301 atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga tcccttcaaa
     3361 taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc ctagttcaat
     3421 cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata acagcgcatc
     3481 acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca tggcttgttc
     3541 aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt gtgctgcctc
     3601 catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg cttttgcaac
     3661 attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta gttttttatc
     3721 tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt
     3781 gcccacttca ataatatcta ctacatcacc aactttcata acatctccta atgcatgttc
     3841 taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag ttccttcctt
     3901 tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc tttgactgac
     3961 atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt aaacatattc
     4021 aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt tccctctgaa
     4081 atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc tttttccaca
     4141 cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa aattctcatc
     4201 ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct ctgcttttct
     4261 gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt ccgatattgt
     4321 aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata acctaggact
     4381 ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc gatataacca
     4441 ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac ttgtatgatt
     4501 cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt ggcataaact
     4561 gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat cgttggtgat
     4621 ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa caagaaccca
     4681 atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat tcctggaaca
     4741 gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc atcgacgaat
     4801 gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga agtcgtcata
     4861 atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa ataatctttg
     4921 cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt agcaaccata
     4981 ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt tttgtcttgc
     5041 ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa ttttgcaaat
     5101 ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc
     5161 tgcgtgattc ctactgttaa gttagaagct actgcccaat aacaacctaa aatcagtccc
     5221 ataaaaatca atacttgtat cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa
     5281 gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg cagtttcgta
     5341 tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa taaaatcatc
     5401 acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc attttgtcca
     5461 aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc aaccaagatt
     5521 ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta accgaccgtt
     5581 gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat atagcctaat
     5641 aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc
     5701 caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat tatattctgc
     5761 catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa ccaatccatc
     5821 tgtcattaat aattcggata atttttggat attttctaga tgttcttctg gatcttttgc
     5881 tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac tttcaaaacg
     5941 gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat cacttttttc
     6001 tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt atttttctac
     6061 acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga tcaatacaag
     6121 acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt aatgaatcaa
     6181 ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa aaacgacgga
     6241 aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa attaaactca
     6301 agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat ataaggttta
     6361 aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc ttttggatca
     6421 gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc attcaatgac
     6481 tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg tgggttttca
     6541 ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc aaataaattg
     6601 gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa taaatagttt
     6661 actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc tggatcttca
     6721 caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat gtctttgatt
     6781 ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc ccatttttgc
     6841 cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt ttgatgaact
     6901 gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat taaagcgtct
     6961 acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt aggctgcata
     7021 ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc attcccatga
     7081 ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc atgacttttc
     7141 agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc cactacttct
     7201 gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac taaccacttt
     7261 tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata tcgttgttcc
     7321 aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta tatgaccgtt
     7381 tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat aaagacagat
     7441 atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt ccctcaggag
     7501 gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga aattttggaa
     7561 ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct tgatgtatct
     7621 attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat catacgcaat
     7681 tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa
     7741 aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta tgatacgctg
     7801 tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc taaccatttg
     7861 acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa ttgtagttat
     7921 attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga catcgcatta
     7981 aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg agtcgatgta
     8041 gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat gatggtcaac
     8101 aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact gacatcaaag
     8161 ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt ttgttctgaa
     8221 aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga ttcattttaa
     8281 gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa atttatcgat
     8341 aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat ccatccaatc
     8401 gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa ataggaatca
     8461 aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg aataaacggt
     8521 attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc aatcaccttt
     8581 accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata tctttctaaa
     8641 taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa gtattccaat
     8701 gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa ttggattgct
     8761 ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac aatgcagacc
     8821 atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac ataaactttc
     8881 gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc tgcttgtaag
     8941 tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa taatgctgca
     9001 tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat ttgatttaaa
     9061 agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata agattcttgg
     9121 tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat tttgtcttct
     9181 tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag cgcacttaag
     9241 atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc ttgttcacaa
     9301 atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc ttttagtaat
     9361 gctccatcta ctttaatcaa aacaatttcc ttccctttaa
//
LOCUS       DACIRY010000107         8874 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.110, whole genome shotgun sequence.
ACCESSION   DACIRY010000107 DACIRY010000000
VERSION     DACIRY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.110"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(221..1177)
                     /locus_tag="IYE22_002151"
     CDS             complement(221..1177)
                     /locus_tag="IYE22_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ9507345.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IYE22_002152"
     CDS             1281..1883
                     /locus_tag="IYE22_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ9507346.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IYE22_002153"
     CDS             complement(1971..3470)
                     /locus_tag="IYE22_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ9507347.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IYE22_002154"
     CDS             complement(3486..4010)
                     /locus_tag="IYE22_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507348.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IYE22_002155"
     CDS             4308..5057
                     /locus_tag="IYE22_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ9507349.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IYE22_002156"
     CDS             5048..5833
                     /locus_tag="IYE22_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ9507350.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IYE22_002157"
     CDS             5802..6518
                     /locus_tag="IYE22_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ9507351.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IYE22_002158"
     CDS             complement(6620..7639)
                     /locus_tag="IYE22_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ9507352.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IYE22_002159"
     CDS             complement(7658..8716)
                     /locus_tag="IYE22_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ9507353.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2669 a         1791 c         1590 g         2824 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac taga
//
LOCUS       DACIRY010000108         8865 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.111, whole genome shotgun sequence.
ACCESSION   DACIRY010000108 DACIRY010000000
VERSION     DACIRY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.111"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            930..1403
                     /locus_tag="IYE22_002160"
     CDS             930..1403
                     /locus_tag="IYE22_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ9507354.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1412..1639
                     /locus_tag="IYE22_002161"
     CDS             1412..1639
                     /locus_tag="IYE22_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9507355.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2275..2646
                     /locus_tag="IYE22_002162"
     CDS             2275..2646
                     /locus_tag="IYE22_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507356.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2902..3144)
                     /locus_tag="IYE22_002163"
     CDS             complement(2902..3144)
                     /locus_tag="IYE22_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ9507357.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3176..4078)
                     /locus_tag="IYE22_002164"
     CDS             complement(3176..4078)
                     /locus_tag="IYE22_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ9507358.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4091..4279)
                     /locus_tag="IYE22_002165"
     CDS             complement(4091..4279)
                     /locus_tag="IYE22_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ9507359.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4293..4856)
                     /gene="amaP"
                     /locus_tag="IYE22_002166"
     CDS             complement(4293..4856)
                     /gene="amaP"
                     /locus_tag="IYE22_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ9507360.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4894..5787)
                     /locus_tag="IYE22_002167"
     CDS             complement(4894..5787)
                     /locus_tag="IYE22_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9507361.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5865..6803)
                     /locus_tag="IYE22_002168"
     CDS             complement(5865..6803)
                     /locus_tag="IYE22_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ9507362.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6837..7187)
                     /locus_tag="IYE22_002169"
     CDS             complement(6837..7187)
                     /locus_tag="IYE22_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ9507363.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7220..8122)
                     /locus_tag="IYE22_002170"
     CDS             complement(7220..8122)
                     /locus_tag="IYE22_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ9507364.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8115..>8865)
                     /locus_tag="IYE22_002171"
     CDS             complement(8115..>8865)
                     /locus_tag="IYE22_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ9507365.1"
                     /translation="RRIKFYVIDDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYE
                     DFLVSDHITTTAYLRISLPNLLAKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGA
                     IIDPGQVKALERLGIDSDDLYFNSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHD
                     QDALNAVLYEDWEQLHPKWNMQTSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDK
                     PWNTLKDHPYTNLYLKKLAHSTLTKVGEINE"
BASE COUNT         2483 a         1679 c         1468 g         3235 t
ORIGIN      
        1 tacaataaat aactttagtt actttaatgc gttcttactt tatttttata ttatcatttt
       61 tcttattcac ttctttggtt tatactattc ttataaataa aaacgaggta tgtcctatga
      121 tgaaaagatt gtactattca ttgatcataa cgattggcta tttgattgta tcaaatctag
      181 gaaatatggt atttggtatc agtaaagaat tcagttggac tactacatta tgggaatctc
      241 tattcttttt catcttcgta tttttgttac aaaattatcg aaaaaaataa acatgtgcca
      301 tctatctgtt ggcacatgtt ttaggattta tggtactttt tattcggcaa tccattttat
      361 ttttctactt cttcttaata cctctaacac ttttgattta taaactaatc caatgcatct
      421 acttgcgctt attaagctat ttcagctaga tagtaccacc ccatgcgtca atgcttgatt
      481 ttccttttgt ataacattgt ataagcaata aaggacaaat aagtggtgat tacaccaaac
      541 cattgaataa gaaaaattct gatacgtaaa taaacatgaa cttgaaaaaa ttaatgaatc
      601 ggttagctac tacgtagttg tcttttttct catacagttc tgttaactta cacttctctt
      661 cgctagataa taaaaaatcc tccaactgta ttgtccaata taatcccagg catctaaact
      721 agcattatag aagagacaca catagaatct aaaataactg gttttaaatg tttcttacaa
      781 tcaaaaaaag ctgttaaatg cactccacac cttcatactc tatcattttt tataaacgat
      841 atatttttat tgtttttcga taaaaaatag ttgttttaca ataattattc tgctatattt
      901 tagctatcaa agataaaaga gggataaaaa tgaaatttaa aacattgcta ttaaaatttg
      961 tcgttttagg tataagtgcc gtatttattt tgttcggcgt acttttttat actcagatcg
     1021 ctacaagcga aaaaggattc aattttgatt ggcagaccac attatttgtt tctgtcattt
     1081 actttgccct tctcctaggg atcgttattg cttatttctt attgaaacta ttgaaaaaaa
     1141 tcgaagataa ccaagctttt tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa
     1201 ccattttgtt tattggtttc gccttttttg gcttacttcc taaatttatg aaagtagcgg
     1261 atttagacga tgctccaggt ttgatgttgg taggaatcgc tgtcgtattc attccttttg
     1321 cagtatacac attaacaagc gtgctggaaa aactgcttga acatgcgatt ttacttaaga
     1381 cagatcatga tttgacagtg tgaggtgcga catggcaatt atcgttaatt tagatgtagt
     1441 attagctcaa aaaaaaatga gtgtgactga attgagtgag aaagtgggca ttacgatggc
     1501 aaacatctct attctaaaaa atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa
     1561 aatctgccaa gttttggagt gtcaaccagg tgatttgttg acctataaaa atgactcgaa
     1621 tactatacca gaagaataaa ataaagtaga acaaacagta aataaaaaat aatctgaatg
     1681 atagatgtgt gagactttta gaagttattt acgtctatta ttcagatttt ttatttcgat
     1741 tgaataccgt tttatctata aataagatat gtactgactc atacaaaggg ggccttttct
     1801 tctccatgta ctatcacttt tcctgataca actggtttcc tgttacgttg tagttggatc
     1861 tgcttttagt ataatacttt attcattcca ccagactgat agcagtttga gcagtctaaa
     1921 taatcatgta gtattacagg ccattctgtt tcataccttg aagtgcttaa aacttcagct
     1981 aatggcccat tgtgttaact cttactccat tcttttttag taagctccca tatatactgt
     2041 aaagccttct atatattatg tcatgttgct ttttaacatt tactatgtag ttttgataaa
     2101 atgatttgaa taacagtgat ggacaattct tttttatttt taacattatt ttgataataa
     2161 tgattattta tttaaaaata cattagataa catttgttac gacacgaaaa agcttcacaa
     2221 acaaatatcg ttattttaaa atgagggcga actcacacaa ttaggaggaa tagtatgcca
     2281 tttattgttt acttttttct ttcattactc gtgatctata ttccattacc aacatttttc
     2341 ctgctcgtca atcgtctagc agagcaacat gatacgattg ctgctcttac gaaactttgt
     2401 ttacttctat ttgtttgtct agattacttt acgagctatt attggctaaa acagacaaga
     2461 gtaaaaaaga gatattattt cttccttgtc tttttcaatc tctttgagtt tctgatccat
     2521 tgctttttac ttcatcagta tcatacgatc catttgtgga tcatctgggt tttcaaagtg
     2581 gtcgtattat tttgtatgtt cacctttcct atctcaagaa aatttaggga ggcattgttt
     2641 tattaaagaa gcttctctgt atcaataaag acacatttaa ggaaaaactt ttaaaaatct
     2701 aattgggaat ttgcccgcat aaataagagg ctgtgacaaa agtcttgtca cagcctctta
     2761 ttccgtataa acagtgttca aaagcttgca tcttcgataa tatgcccaac caaatgaaaa
     2821 atatgagaag ctatttttgt ttggctgttc ttattacttg atgaagaaca gctttttcac
     2881 gacctctttt taaaagtctt tttacagttt tcgagcaaag aatgatacga ttagtactaa
     2941 aatgatggca ccaatcaatg atggcacgat tgccattcct gcaatacttg gtccccaaga
     3001 acctaataat gcttgaccaa gtgctgatcc aactaaacct gctacgacat ttgcaataat
     3061 ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt gctccgataa ttcctccgac
     3121 gataagtgac caaataaatc ccatgtttcg ttcctcctaa tgttttactt ccttattact
     3181 gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc gcttccagct gctgatttta
     3241 ctttatctgt ttgattcgaa gcaaattcac cggttttttt cgctgcgtca gttactttgt
     3301 cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt tttgatgtcc actacgttag
     3361 cgttgacttc gatcacttct aaatgtgtca tattttttac ttctttgccg attacttctt
     3421 gcatttcttc aaagattttt tcgatatctt tgccatattc cacaatgaca tccatatcaa
     3481 ctgctacttg ttttttaccg acttctgttt cgatcccagc cgttacatta tctgtattca
     3541 caagttttcc tgccacatta gagaagaaac caccgtttgc agataataaa ccgtctactt
     3601 gttcaattgc gattccgatg attttttgca caactttatc ttcataattc agctcaccgc
     3661 gcattgaatc ttcctgttca gaatcatgct ttccttggtt atttgtatga tcagaaccaa
     3721 gttttccttg tccgtttgtg tggtcagaac taagtttttc ttgtgctttc gcatggtcag
     3781 aaggatgttt ttgctcttcc acaggtttag aaggatcgat tgcatcatgt tgttctggaa
     3841 ctttagattc tggagttcct ggatgatgag gtgcatgctt ttctgcatca tgtttcttct
     3901 cttctactcc ttgttgaacg ttagggacta ctggatctgg atgtgcagat actggtgctg
     3961 ttttgttctt ttcatcgttc acaccatttg attggtttgg tgtcgttggg tctggatgtg
     4021 cagacactgg tttttggttg ttcattttat tgttattatt tttgttattg ttattcatgg
     4081 atgtttcctc ctaagattta aaatagtgat ctaagatttt cgtttgtttc agataaaaac
     4141 cgatagcgac gccgatcgct gccatgatca agatcaataa ggtcttgaaa aagcccacgg
     4201 tgatccataa aacggctaat atcaatccaa gtaccccaaa gatgataggg agcttgtatg
     4261 tcgaaaatag ttctttcatt tttggtcccc ccctattcta ctcgtttgcg gtcattgctt
     4321 cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata ctttcactgg ctcttttgtt
     4381 cccaatatgt gttgtacttc attttcgatt tctttcatca atgttcctgt tttaccaatg
     4441 agtgctgaag ttcgtttcaa ctcccctttt acattgacgg cgatcttgtt tttcgttgct
     4501 ttgacattta cttttggtga atggatgaat tcatcttcat gcaagtgaga acgaaccatt
     4561 ccttcaattg cttttttatc caacgtcaac tccccatcac catgtttcag tagaaagttt
     4621 ccgattgttt ttggataaaa caggaaaaca agtaacgtga taattgtcaa aatagcaaat
     4681 gcaacggcaa gccaaaatac atattgttgc acgtatgaat tagtgatgga aaagaatcga
     4741 aacttctcta gctgaaaagg caatggatag gttgcactta atagtatcgc aaacaaaagc
     4801 acagatagca aaagtaatgt tccgatcact ccgatcgttt ttacaccttt attcattttc
     4861 gcacctcctc ataggggtga aacgatcaac ttcttataag ttgatcgctt ctccaccagt
     4921 tactccgtaa atttgggctg taacataact tgctttattc gatgctaaga aaacataaac
     4981 tggtgctaat tctcctggct gacctgcacg ttctagtaaa ctttgctgac caaattcagg
     5041 gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt gtccagattg gaccaggtgc
     5101 tacaccattt acacggattc ctttaggtgc aagctgtttt gctaaaccga tcgtaaagtt
     5161 agcgattgaa gcttttgttg ctgcgtaatc taataaatgt tcgcttggat tgaatgcttg
     5221 aacagaagtt gttgttacga ttgctcctcc tgctgggata tgcggaacag cttctttcac
     5281 taaagcaaac atagcgatga tgtttatcat aaatgtgtct ttgacttgct caagtggtaa
     5341 ctcagcaata gactgacaag aaatttgctg tcctgcgttt agtaccatcg tatctagccc
     5401 gccaagttca ctcacggctt tatctatgat ttctttaggt gcttgttcgt cacgaagatc
     5461 aaaaggtagt aagacaatct tacgtcctgc aactttcacg tattttgcta cttcctctgc
     5521 atctttttct tctcctggga agaattgaag agcgatatct gccccttctc tagcaaatgc
     5581 aatcgctgct gcacgaccga tccctgaatc tccaccagtg attagtacac ggcggttttc
     5641 tagttgatta ttgccttcat aggattcttc tccacaatca ggaacaggaa tcatattctg
     5701 ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc tccgtatggt acaagtttcg
     5761 cgggtcgatt aattttggtt ctgtcattgc tgtattcctc ctagttttta aattgtattg
     5821 ccaaaacaaa tttctttgtt tgatacctgt tttaaataga ttctttaatt tgtataatct
     5881 ttccggtata aatggtaacc taaaactact gctgctatca aagagccaaa gactacgagt
     5941 ataaagccta gagcagaact tttacccggt aatcctcctg tattcattcc ccaaatccct
     6001 gcgaacaacg ccggaacgga aatgattaat gcagctgaat ctagatattt catgatatga
     6061 tttagattat tgtccatcat cccgttgaat agatcaccaa tactctctaa taaatctcga
     6121 tagatctcga tcattttttc tgtttgtcgc tgtctcaaat ggacatcata gagtagctgt
     6181 tcatcgtcta aacgtttgat gaaagtttca gaattccata attgatccat tgtttcccgt
     6241 tgatctctta aggtatgatc gatatatacg atgtctcttt caagatctgc ctgtttatac
     6301 agttcttcat tttccgttgt tttacgagct gctttatcca aagcgtcaat tcgctttttc
     6361 atctctctta gttctttcac gtaatgggtg taaatatcta ataatgtata cgtgatgatc
     6421 ttttcaaacg agtttaaatg atttccatgc tttgtcagca gccgatccac aaattcggtt
     6481 tttctatcga tacaagtaat caagagatta tcggaaagta caaaagatat tggcgttaat
     6541 tttttttcga tacgattatc tgactgatct agattaatca ctaccaaaga aaatggatgc
     6601 tctagtttcg tctcataaag gcgctccatc cgagaaactt cttccggata ttcggttcct
     6661 acgaaaatat catcaggaag ctcgaattct tcgattgctt gctcaatctc ttcgggcttt
     6721 gcactatcaa gtacgagcca attatatttt ttttcatctg tttctattac attcttttct
     6781 tcatccacaa agtagtatct caatgtatac ctcctttttg gactttctcg aaactattag
     6841 ctgaagaagc agttctttaa tttgatatta taatcgagta ctgcgatttc attttctaat
     6901 gaaaagctgt caagctgttt gttgaagtcc atgttcaatc gtttaccgaa ataattttgc
     6961 tctaatttag atgtatagat gtccaataat ggatgctcga ttttagtcgc atattctcca
     7021 aattccgaaa caaaaggaat gatttgaaaa aagctgcctt ttgcaaaagc acctttgtcc
     7081 cttagaaatg gatcggaatc attgataagc gcgagtgcga tttttgtatt ttccccaacc
     7141 ttttcaatta acgtctctac tgcattttta gacattgtaa tttccattcc tactccccct
     7201 cttcagttgt tgtcttctat tatttaacaa ctacatctgt ttttggttga acttttgcat
     7261 attttggata tttagggaat ttagctacac aacggaaagc cgtcatccct gcatattttt
     7321 cgtaatattt cttcgttggc ccttcaaaat ccttgctcca cggtttcaca tgtcctgaaa
     7381 aatggataat gtaaggattg ttgcgagttt cttcgtattc acgttcacct gctgctgttg
     7441 gatgtttttt tgcttttgcc atgatatagc cttgtgcatt ccaacgcgga tgaagtggta
     7501 accaacggtc atgtaagata gcatttaatg catcttggtc atggaatcgt aatttttctg
     7561 gattatgttc gatgaaatct aatacttttt gcgtaatatt ttcatctaac cattttttca
     7621 cattgatcag catcaaacct gagttgaaat aacgcattga tttagctgaa attttcattt
     7681 tttccagtcg ttgatggaac ccagcgtctt caacagctgc tacgattgaa tcaccgaagt
     7741 caagacgcca taatttagag atatcttgta aagcgatcat gtcgcaatcc atatataaga
     7801 tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc aatacgataa taagctgttt
     7861 ctggaatacg atcgctgatt accacatttg taaagaattc tttattgatt ttcaagaatt
     7921 ccacatctga gttcattcgt gcgtttttca ctgagaagcg taaaagctgt ttactttcaa
     7981 aatcgatatc atcgtcaatc acatagaacc gaacaaattt agacgggttg caattatcta
     8041 atactgagac aaataatgca gctaaatgtg gtacgaattt tgtgttacaa cttgcgacta
     8101 ctgctatttc ttttttattc attgatctcg cctactttcg ttagagtact gtgtgctaat
     8161 ttttttaagt ataaattggt atacggatga tctttcaacg tattccaagg tttatcatgt
     8221 cctgtaaagt gaacgatcga aggtttttca tttccttctt tatactgacg ttcatatttt
     8281 tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct gcatgttcca ttttggatga
     8341 agttgttccc aatcttcata aagtacagca ttcaaagcat cttggtcgtg atagatgatt
     8401 cgatcgccat tttcactgag gtaatggata gttttttctg taatttcttt tttattccat
     8461 tgatcaatgt caatcaccat cactcctgaa ttgaagtaca agtcatcgga atcgattcct
     8521 aatctttcca aagctttcac ttggcctgga tcgataatag cgccgattgt ttttccattt
     8581 aacggctcat catatagttt tacgatatcg tctagtacta acacatctga atctaagtaa
     8641 aggactttct tataatcctc tttagctaat aagtttggta aagaaattct taagtaagcg
     8701 gtagttgtga tatggtcact gactaggaag tcttcgtaga tatctttttc cactgtcagg
     8761 aattggatac ttgcattact tgaatattta tttaccgttt cttccaatcc ttctttacta
     8821 tattctgata gaccatcgtc aatcacataa aattttatgc gtctt
//
LOCUS       DACIRY010000109         8735 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.112, whole genome shotgun sequence.
ACCESSION   DACIRY010000109 DACIRY010000000
VERSION     DACIRY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.112"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(474..1061)
                     /locus_tag="IYE22_002172"
     CDS             complement(474..1061)
                     /locus_tag="IYE22_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507366.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1236..2558
                     /locus_tag="IYE22_002173"
     CDS             1236..2558
                     /locus_tag="IYE22_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9507367.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2713..3489
                     /locus_tag="IYE22_002174"
     CDS             2713..3489
                     /locus_tag="IYE22_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ9507368.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3482..4051
                     /gene="rnmV"
                     /locus_tag="IYE22_002175"
     CDS             3482..4051
                     /gene="rnmV"
                     /locus_tag="IYE22_002175"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ9507369.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4044..4928
                     /gene="rsmA"
                     /locus_tag="IYE22_002176"
     CDS             4044..4928
                     /gene="rsmA"
                     /locus_tag="IYE22_002176"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ9507370.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5379..6008
                     /locus_tag="IYE22_002177"
     CDS             5379..6008
                     /locus_tag="IYE22_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507371.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6132..7241)
                     /gene="ugpC"
                     /locus_tag="IYE22_002178"
     CDS             complement(6132..7241)
                     /gene="ugpC"
                     /locus_tag="IYE22_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ9507372.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7450..7872
                     /gene="mgsA"
                     /locus_tag="IYE22_002179"
     CDS             7450..7872
                     /gene="mgsA"
                     /locus_tag="IYE22_002179"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ9507373.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8195..8731
                     /locus_tag="IYE22_002180"
     CDS             8195..8731
                     /locus_tag="IYE22_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ9507374.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2766 a         1543 c         1790 g         2636 t
ORIGIN      
        1 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
       61 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
      121 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
      181 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
      241 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
      301 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
      361 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
      421 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
      481 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
      541 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
      601 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
      661 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
      721 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
      781 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
      841 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
      901 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
      961 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
     1021 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
     1081 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
     1141 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
     1201 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
     1261 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
     1321 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
     1381 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
     1441 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
     1501 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
     1561 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
     1621 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
     1681 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
     1741 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
     1801 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
     1861 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
     1921 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
     1981 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
     2041 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
     2101 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
     2161 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
     2221 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
     2281 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
     2341 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
     2401 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
     2461 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
     2521 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
     2581 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
     2641 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
     2701 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
     2761 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
     2821 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
     2881 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
     2941 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
     3001 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
     3061 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
     3121 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
     3181 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
     3241 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
     3301 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
     3361 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
     3421 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
     3481 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
     3541 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
     3601 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
     3661 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
     3721 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
     3781 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
     3841 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
     3901 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
     3961 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
     4021 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
     4081 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
     4141 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
     4201 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
     4261 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
     4321 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
     4381 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
     4441 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
     4501 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
     4561 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
     4621 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
     4681 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
     4741 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
     4801 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
     4861 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
     4921 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
     4981 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag
     5041 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
     5101 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
     5161 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
     5221 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
     5281 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
     5341 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
     5401 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
     5461 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
     5521 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
     5581 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
     5641 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
     5701 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
     5761 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
     5821 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
     5881 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
     5941 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
     6001 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
     6061 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
     6121 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
     6181 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
     6241 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
     6301 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
     6361 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
     6421 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
     6481 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
     6541 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
     6601 ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
     6661 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
     6721 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
     6781 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
     6841 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
     6901 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
     6961 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
     7021 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
     7081 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
     7141 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
     7201 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct
     7261 tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat
     7321 gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat
     7381 ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga
     7441 gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa
     7501 ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact
     7561 gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta
     7621 ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt
     7681 cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta
     7741 tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga
     7801 ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca
     7861 ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc
     7921 tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa
     7981 caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg
     8041 aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga
     8101 tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga
     8161 tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg
     8221 tatgattgct attatgggct tattgattgc attgatggtc actttgtccc gactggtagc
     8281 aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat
     8341 tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc
     8401 cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc
     8461 aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac
     8521 gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta
     8581 ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg
     8641 gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat
     8701 tttaagaagt ttagcgatcc acgctaaata aaaaa
//
LOCUS       DACIRY010000110         8652 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.113, whole genome shotgun sequence.
ACCESSION   DACIRY010000110 DACIRY010000000
VERSION     DACIRY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.113"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            91..870
                     /locus_tag="IYE22_002181"
     CDS             91..870
                     /locus_tag="IYE22_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507375.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            911..1855
                     /locus_tag="IYE22_002182"
     CDS             911..1855
                     /locus_tag="IYE22_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9507376.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1871..2650
                     /locus_tag="IYE22_002183"
     CDS             1871..2650
                     /locus_tag="IYE22_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ9507377.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2663..3361
                     /locus_tag="IYE22_002184"
     CDS             2663..3361
                     /locus_tag="IYE22_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ9507378.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3389..4153
                     /locus_tag="IYE22_002185"
     CDS             3389..4153
                     /locus_tag="IYE22_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ9507379.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            4166..5983
                     /locus_tag="IYE22_002186"
     CDS             4166..5983
                     /locus_tag="IYE22_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ9507380.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            5997..7220
                     /locus_tag="IYE22_002187"
     CDS             5997..7220
                     /locus_tag="IYE22_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAQ9507381.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            7234..7968
                     /locus_tag="IYE22_002188"
     CDS             7234..7968
                     /locus_tag="IYE22_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ9507382.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
     gene            7985..8596
                     /locus_tag="IYE22_002189"
     CDS             7985..8596
                     /locus_tag="IYE22_002189"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507383.1"
                     /translation="MVIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRS
                     YTEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNIL
                     MRLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIAL
                     ETDALNNDVVNNFYKKNEFILERKYSTNEGRIMNEYRFYMEKN"
BASE COUNT         3064 a         1447 c         1777 g         2364 t
ORIGIN      
        1 ttaattatgc ttttaaaata aaaagcgctc attctgatgt gaatgaatca catctttatt
       61 tatctatcat caatatttag gagtgaatct atggaaaagc cgttcgtcaa gttattggct
      121 actgtggcgg taggcacagg cgctattgtg atttgcttgt ttggatacca tctcaataat
      181 cagcggcaac atcatcaacg gatcaattac gcagaatctg ccatcaccaa tcaaaaagac
      241 accgtgacct ctttatcaaa agaagtagat aagctgtaca gtacaaaaga aaaaattttc
      301 ctaaatcctg aaataacaga agaaacagtt tcaaatttgt cacataaact atcttctatc
      361 aaactttcag cagatgatta tgacatcaag gaatcagaat tacctaaaga agctgctgcg
      421 atccaagaag aaaagaaagc agtcttaaca cagttagagg atgcagaatc aaaactgaag
      481 atccaaacgg ctgtcaataa attatttact aaaaacgtat caaactggca gcaagctgtg
      541 gatgatgtca tcatcaaaga aaagttggca agtgcagatg tcgcccatgt acgtgaaaat
      601 atgagctttt ttaaggatag tgcttggaaa acggtcgtta tgcaatatct aggattcgct
      661 gatacccaaa ttgcacaagt cacgcaacta gatcagctat tcgatacgat gctgaaagac
      721 ggtcaagtaa cagctactgc tacttacgat caatatttga ccgcactcag tctcatcgaa
      781 cagatccgga atgaaaaaat aagagccgca tacgcgtcaa aagcagaaac agtcgctcag
      841 caaatgggct atagcaatac gagctattaa taagcgagaa ttcaagcaag gctagcaaac
      901 ggaggaaact atggcaaaac gaagaagaag atcaagaggg aaaaagatat ttctaattat
      961 tttagcaatt cttgtcgtat tagtattagc aatcgtagga gtaggagcaa aattatatct
     1021 agatctctca cattctattg aaaaaactta cgaatcagtg gaacgaaccc aaggagaacg
     1081 aacaaaacca gttgatctgg acaaacaaga atctttctct gttctattga tggggattga
     1141 tacgggagat ttaggtcggg tcgatcaagg aagatcagat acgatgatgg ttgccacagt
     1201 cagtcctgaa gacaaacaga caacgattgt cagcattcct cgtgatacgt atgtcgacat
     1261 cgttggacat gacacaaaag acaagatcaa ccacgcttac gcattcggcg gagctgccat
     1321 ggcgatggat accgtacaaa aatatgtaga catcccaatc gatcactatg tttcaatcaa
     1381 tatgaaaggt ctaaaagaac tcgttgatgc agttggtggt atcgaagtca ataatgacct
     1441 caccttctca caagatggct atgattttac gatcggtaaa atcacattga acggggatca
     1501 ggcattgtct tattctcgta tgcgttatga agatccaaat ggtgactacg gcagacaaga
     1561 acgtcaaaga aagatcgtag aaggtgtagc gaaaaaagtg ttgagtctga acggtgtaag
     1621 caactatcaa tccgttttga atgctctaga gtcgaatatg aaaacagaca tgagctttga
     1681 tgaaatgaaa aagattgctt tagattatcg ggatgcattt ggtacgatca agcaagatca
     1741 gctgcaagga gaaggcttca tgcaagacaa tatctcatac caaaaagtca gcgatcaaga
     1801 acttgatcgt gtgaaaaaag aattaaaaga tcaaatgaat ctagagaata aataggaaag
     1861 tggagaaaaa atggaagaga caatcagctt gaaagaaatt tttgaaacat tacgcaaaca
     1921 cgtgataaca atcctcatct caatgtttgc tggcttggcc atctcaggca tcgtgacgtt
     1981 tttcatcttg acgccaaaat acagctcaca agcccaattg atcgtgacac tgccgcaatc
     2041 agatactgca aatgtcaatg atgtcaatac gaacctgcaa atgatcaata cgtataagga
     2101 tatgatcgtc agtgatctag tactaaatga agtcaaagat cgtttagaaa cagaagacaa
     2161 tgtaaaaatg actgctggac aaatcaaaga tgccatctcc gtcaatcagt cacaaaattc
     2221 acaaatgttc tcgatccaag caacaagcac aaatgcagtg acggctcaac aaatcgctaa
     2281 tacaacgtca caagtattcc aagagaacgc caaagacgtg atgaatgtcg ataaaatctc
     2341 gatcatctca ggtgcagttg ccaatacgac acctgtttcg ccaaataata aattgaatct
     2401 agtgattggc ttagtgttag gaatgatggt aggcgtaggt ctagctttct tatttgaatt
     2461 actcgaccgg acagtaaaag atgaaaaata tgtgacggat acattgggct tcccgatctt
     2521 agggactgta ccagaaatgt cagcgaaaga attgaatgct ggtatccgta aaaaaggacc
     2581 agctccgaca caaccaaaag ctacaaacga taaaacagcg ccatcacgca gaagccgctc
     2641 aagagtctag gaggaaagaa aaatggcacg aacacagaaa caaaaagcag cccaaactca
     2701 tgcagtcagt ttggtcacac tcacaaaccc ctcttcacca atcgctgaac aatatcgtac
     2761 gattcggacg aatatccagt ttgcctccgc agctggtcag caaattaaaa cgattgttgt
     2821 gacttcatca ggacctggag aagggaaatc gaccactgca gcaaacattg ccgttgtttt
     2881 tgccaaatct ggtcaacggg tgctgctagt cgatgcagac ttacgtaagc ccgtcatcta
     2941 taaaacgttc aagttgaaca acgcaagcgg attaagtacc gttcttagtt catcaggaag
     3001 tgtagcagat gagatccaac gaacaccagt cgagaatttg tcgattctgc caagtggacc
     3061 taaaccacca aatccatcag aactactaag ttcgccaaga atggatcaga ttttagcaga
     3121 agcaagacaa ctatttgatg tagtgatctt cgacatgccg ccagtagttg ccgtaacaga
     3181 tgcacagatc atgtcttcca aaacggatgg caccttattg gtcgtacgtg aaaatatgtc
     3241 acggaaagaa tcattgacta aagcaaaaga attgctggat atggtacaag cgcgagtcct
     3301 tggtgttgta tacaatgggg cagaacacag caaagatgct ggctattact acgggaatta
     3361 agaaataaaa agagaggaga caagagaaat gatcgatcta cattgccata tcctgccagg
     3421 aatcgatgac ggagcagaaa acatagatgc cagtatcgcc atggctgaaa aagcgattca
     3481 acaagggatc acacatattt tatgcacacc ccatcataat aatggaaaat acagcaatga
     3541 aaaatcacaa gtcatttctc tagtcgcttc gttacaagca gaattagaga aaagacaatt
     3601 gcctttgacg ttattggaag gacaagaagt acgtattacc ggaacactga tcgaagacat
     3661 cgaccgagat gaaattttat ttacagattt agatgataca tatcttctaa tcgaatttcc
     3721 gacactggaa gtgccgttat atgcagaaca gctctttttt gagttgttac aactaggaaa
     3781 gacaccagtg atcgtccatc cagaacgcaa cgcgcatttt cgtaaagacc cgaatcatat
     3841 gctgccattc ctagatatgg gatgcttggc ccaactcacc gcaccaagtt atgtcggtgt
     3901 atttggaaaa gacatccaga aaacagccaa aatgatggtc aaacataatc ttgtgcagat
     3961 ggctgcttct gatgcccatg gggtctctaa gcgcaccttt tacttaaaag agtgctataa
     4021 gcagattgcc aaggactttg gtaaggaaaa agtagtacag atgaaacaag tcgcaaagga
     4081 tctgatcaac ggggatgaaa tcagttatcc agtctatgaa gagataaaaa agaagaagtt
     4141 tggtttattt tagggggagt gtaaggtgtt tgtattaaca agaagaagaa aaattgccac
     4201 attggttgtt gtggacagtt tgttattgat cgctgcaaat attgcagcag ctaaattcat
     4261 gaagccgttt gtagctatac caatggacct tattttaatc tctaccggtt tgtcgattgg
     4321 tttttattta ttttatggta gtttattcaa agttttcact cggatcaatc gctatacaaa
     4381 tttaagagag atcattgcaa tttttggttc actatctgca agtgacgtct caagtatcct
     4441 catactgctg ttcattaatc ggcaatacag tttacggtta gttatctttg cttatctgct
     4501 ttcgctctta ttgatcatcg gtagccgatt gatctggcgt atctacatcg aaacaaaaaa
     4561 tatgagatat gtatcagcgg attctgctaa aaatacattg atcgttggtg ctggcgaagg
     4621 cgggcgtatt ttatacaaca gctttctggg atccaaaacg gctcaagata tccaagttgt
     4681 cggatttgta gatgatgatc cgaataaaag aaatacgtat ctttcaggaa aaaaagtatt
     4741 aggtgcatta aaagatattc cggaattgat tgaaaaatat gacattcaaa tggtaacgat
     4801 cgctatccct tccttatcaa gaaaaaaatt acgaagaatc ttcgagttag tcgaatccgc
     4861 tcacgtgaaa gtcaacacga tgccttctat agaagaactt gcttcaggaa aaattagcgt
     4921 atcaaaatta aaaacaattg acgtagtgga tttactggga agagatgagg taaagctgga
     4981 tattgaatcc atcaaagatc agatcacaga caaagtcatt cttgtcaccg gcgcaggagg
     5041 atcgatcggt tcagaaatct gtcggcaaat cattcaattc aatcctgcaa agctattgct
     5101 tttaggacat ggggagaact cgatctactt gattgaccgt gagttacgca cgcatcatca
     5161 aaattgtcct acagaaatcg taccgatcat cgcagatatc caagatcgcg agaaaatcaa
     5221 tgagatcatg gaacaatatc atccagatat cgtctaccat gcagctgctc ataaacatgt
     5281 accactgatg gaatataatc caaaagaagc agtcaaaaac aatattttcg gtacaaaaaa
     5341 tgtagcagaa gcagctaaag cagcaaaagt gaaaaacttc gtcatggtat cgacagataa
     5401 agctaacaat cctccaaatg tcatgggttc aacaaaacga atcgctgaaa tgatcgtgac
     5461 tggattgaat gaagaaggct gtacgaaatt ctcagctgtc agatttggga atgtccttgg
     5521 ttctcgcggt agtgtgattc cagtattcag agaacaaatt gctaaaggtg gtccaatcac
     5581 tgtaacagac tttcgtatga cccgatactt catgacgatt cctgaagcta gtcgtttagt
     5641 tattcaatct ggcgcattag caaaaggtgg cgaaattttt attctagata tgagtgaacc
     5701 agtgaagatt gtagacctag ccaaaaacat gatccgattg agcgggtata gcgaagacga
     5761 aatcgaaatc attgaaacag gtatccgtcc aggggaaaaa ctatatgaag aactactgtt
     5821 ggataaggaa agaaatgatg aagcagttta tgagaaaatc tttgtaggaa acatcaaagg
     5881 ttactcaata caaaaagtga tggattttgt caagagcctg ccgcaagacg atgaacaatt
     5941 ggctaaagat atcgtcactt ttgcaaatgc ttcgaataaa taggggggaa atataaatga
     6001 ataaattaga aaaacgtcag ataccatttt caccgccaga tattactgat ttagaaataa
     6061 aaaacgttgt agaagtatta aaatctggat ggataactac gggaccaaag acaaaaaaat
     6121 tagaaaaaaa aattacaaag tggttaggaa cttctaaagt agtttgttta aattcacaaa
     6181 ctgctgctgc agaaacaggg ttacgtttgc tgggaatagg agaaaatgat gaggttatcg
     6241 tcccagctta tacttattct gccagtgcat cggttgtatg ccacataggc gcgaacctag
     6301 tattaataga ctgtcaacaa gattctttgg agatggatta cgatgcatta gaagcagcaa
     6361 ttacggagaa gacaaaagtg attattcctg ttgatcttgg tggtatacct tgtgactacg
     6421 accgtatttt tgagattgtg gagaaaaaaa gaaatttatt tcaacctagt aatgagattc
     6481 agaaagctta tggtcgtata attgttatgg cagattctgc tcatgcattt ggagctaatt
     6541 ggcatgatca gatggttggt aatgttgctg acttgacaac attttcattt catgctgtta
     6601 agaattttac aactgcagaa ggtggagctt tagtatggaa agagcacgac aaactagata
     6661 atgaggcaat gtatcatcag tgccagctgc tttctttgca tggacagtca aaagatgcac
     6721 ttactaaaac acagttgggt gcatgggaat atgatattgt gggtccatgg tataaatgta
     6781 atatgactga tgttactgca tcaattgggt tggcacaatt tgaacgctat cctaatattc
     6841 tgaaaagaag aaagaatata attaagaggt atgatgaagc ctttcaacaa ctaggaattg
     6901 aagttcttat gcattatacg gatgattata gttcttcagg ccatttatat attactagag
     6961 tacctaacat tagttctgag caaagaaatg aaataattat aaaaatggca gaacaaggga
     7021 ttgcttgcaa cgttcactat aaaccacttc caatgtttac agcatataaa aatatggggt
     7081 ttgatattaa taactatcct aatgcctatg ctcattttgt aaatgagatt acattaccat
     7141 tgcacacgaa acttacagat gaagaagtag aatacgtaat taataactat agtaagattg
     7201 taaaggattt ggttggatga taggagaggc aaaatgagaa agtgggaaga tttgccaaaa
     7261 gaaatgcaga atgatgaaat acggtattat tatgatatcc tcagtaaaaa aaaattcagc
     7321 ttagtggtaa aaagagtatt tgatatttta gtgtctgcaa ctttgttact gacactttca
     7381 tggttatttt ttattcttgc aatagtgata aaaattgatt ctcctggacc tgtattttat
     7441 agacaagaac gtattactcg ttatggaaaa aaatttaaaa tttataagtt tcgtactatg
     7501 gtaataaatg cagataaaga tggcactctt gttactgtga aaaatgatga tcgtattaca
     7561 agagtaggaa aggtaatccg taaatataga ttagatgaaa ttagtcagct aattaacgtt
     7621 ttggtcggag atatgtcttt tgtaggaacc agaccagaag ttggaaaata tgtaaagtcg
     7681 tattcaccat taatgttagc cacattatta ttaccagccg ggattacatc agaaactagt
     7741 attttataca aagatgaaga taaattgttg catgataaaa ataacatcga caaaacttat
     7801 atagaaaaaa ttcttcccag taaaatgtat tacaatttga aaagtatcag tgaatttagt
     7861 tgtatgaatg atttgaaaat aatgtgtaaa actatttttg ttatatttgg taaagaatat
     7921 cacgccgact tagaaggtga acgagagttt attaatcaga taaaatgaat ggaggataag
     7981 agttttggtg attgaagaga taaaaagttc ggataaagag ttaattaatt cgattgtcga
     8041 tattcatatg agaacattta caaatttttt tttgactttc atgggaaaaa agtttttgga
     8101 attgatgtat cgttcttaca cagaatataa tgattctggt atattggttg ctattcaaaa
     8161 tgataagcca ataggatttc ttgcatattc tggagatatg agtggtttat ataagtatat
     8221 gataaagaaa agaataatac cgtttggatt ttatagttgt gtagccttta taaagaaacc
     8281 caatatcctt atgaggcttt ttagagcttt tctaaaacca agcgaaacaa aacgtgatga
     8341 aaattatgtg gaattagcat ccattggagt taatccgaaa gagaaatcga taggagtagg
     8401 ttccatgctt atagataact taaagagtag aattgatttt tcaaaatatg aatatattgc
     8461 actagaaaca gatgcattaa ataatgatgt tgtgaataat ttttataaaa agaatgagtt
     8521 cattttagaa cgtaaatatt ctactaatga aggaagaatt atgaatgaat atcggtttta
     8581 tatggagaag aattaattat gaaattattg tatattttaa atgtagctaa taaagtaaat
     8641 aacttttcta aa
//
LOCUS       DACIRY010000111         8574 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.114, whole genome shotgun sequence.
ACCESSION   DACIRY010000111 DACIRY010000000
VERSION     DACIRY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.114"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(151..1104)
                     /gene="menA"
                     /locus_tag="IYE22_002190"
     CDS             complement(151..1104)
                     /gene="menA"
                     /locus_tag="IYE22_002190"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ9507384.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1117..2220)
                     /locus_tag="IYE22_002191"
     CDS             complement(1117..2220)
                     /locus_tag="IYE22_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ9507385.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2310..3245)
                     /locus_tag="IYE22_002192"
     CDS             complement(2310..3245)
                     /locus_tag="IYE22_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ9507386.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3547..5481
                     /locus_tag="IYE22_002193"
     CDS             3547..5481
                     /locus_tag="IYE22_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9507387.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5938..6354
                     /locus_tag="IYE22_002194"
     CDS             5938..6354
                     /locus_tag="IYE22_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ9507388.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6368..6934
                     /locus_tag="IYE22_002195"
     CDS             6368..6934
                     /locus_tag="IYE22_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ9507389.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6957..7943
                     /locus_tag="IYE22_002196"
     CDS             6957..7943
                     /locus_tag="IYE22_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ9507390.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8098..>8574)
                     /gene="scm"
                     /locus_tag="IYE22_002197"
     CDS             complement(8098..>8574)
                     /gene="scm"
                     /locus_tag="IYE22_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ9507391.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2520 a         1659 c         1738 g         2657 t
ORIGIN      
        1 cgactttttc acaacctcct gataaacggt gttcaaaagc ttgcatctgc ggtaataagc
       61 ccaaccaaac gaaaaatata aggagctatt tttatttggc tgttcttatt acttgatgca
      121 gggcagtttt ttcacaacct cttcatgctg ttaaatggct tgcataatga ttgttagtaa
      181 tagccccaat acataactgg cattgaataa gaccatattt ttgatggaat agctgaaact
      241 gataggctgg ggtaacgttt gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt
      301 caagaaggtg accaagatcg gccattcata gataccgctc aacaacccca ttaaaatcac
      361 gaaatagcaa gcatacatca atagctggaa taagactgtt cccgcaggac gtccgatata
      421 aaaaactagt gtatagcgat gattttcaat atcacggtca agatcccgca aattattagc
      481 caacatgata ttggcaatcg taaacaccaa gggtaaggaa gctaatacga ttgctgctat
      541 agcccaaaga tttcctacca aatcaaattg tccagatgaa aagttgactg tcagatcata
      601 gacatctgaa ccgaatgtat tgacataaac gaccatggca aaaattccaa ggcccatagt
      661 caccccactg aaaatttccc ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata
      721 gaatactcca ataaaacaac agataaatcc catcaaaagt aaaagccaac ccgtattgat
      781 cgttagaata acgccgataa caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat
      841 caatacagga gatagatttc tgcggccaat gacattttct tcatacttat atacttcaga
      901 atgagccttt tcataatcca tgtaattatt gatagcggta gtcgctaaat caaaaactag
      961 cattccaata aaaaataaaa aagtgtttag ccaatgtatc tcatgaaaaa aagcgataga
     1021 gaataatacc cctaccgcaa aaggaaatac actagccacc tttgttcgta tttcaactac
     1081 ttctaaaaag atttttagtg tcatggttcg tactccttat tttagttcag aacggtcacc
     1141 catcgtgtaa tgggagtcct ccgataattt gaaggtgtct ttgattggat cggtcacata
     1201 tactttatcg tcttttgtca cgaagatcgc ttccgtcccg ttattcaatt cgctttcgac
     1261 gtattccagt ccttttttca cacccattga aaagactgct gtagacaacc cgtcaccatc
     1321 aattgattta tctgttatga tcgtgacact tgcgatatca ttgtcgaacg gataacccgt
     1381 ttttggatca aacaaatgat ggtatttttt gccatctact tctaaataac gctcgtaaat
     1441 tccagaggta actaatgttt tgttccgttc tttaatagaa cctaaaacag atccgcgagc
     1501 catgttcgga tcttggatgc cgactgtcca gtcttggttt tcgcctcttg ggctgtggcc
     1561 taagacatag acattaccgc ccaagtcaac gatggcagtg gtcactcctt gttttttcaa
     1621 gactttcact acttcgtccg taatgtaacc tttggcgatt gcacctaaat cgatgatcat
     1681 tcctttttct tttagataaa cagttttttc tttgtcattt agttcgatct tatgataatc
     1741 cactaatttc aatgcctgat cgatttcttc ttgggaaggt tttctggcgt catcaaaacc
     1801 gattcgccat aattgtgtga ttgcaccaat cgccatatcg aatccgcctt gagaatcttg
     1861 gctatattcg taggcccgct tgactaacgt gtagacgtca tcagaaactt ttactggttt
     1921 gatcccagct tgctcattca cttcatcgat ctcagaacct ttttgattga tcgtgatttt
     1981 atcaccaagt tcttttactc gatcgaaggc aggtttcaat gctgattctt tgccttcatc
     2041 atacacacgg atacgaacgt aagtccctaa caagaactgt tcatcagaat atggatcttt
     2101 caatatcttg gtagctgctg aactttcagt ggttgtttta tttgctgagt tgctgcagcc
     2161 agttagcacc ataaaagaga tgcccagtgt gattaaggaa aatagtagtt ttttccgcat
     2221 ctttttcctc tcttcatttt gaaaggtgag acgactgcct aatctggcta tcgtcccacc
     2281 ctccaacttt tattagtatg attatgtgat tatttttctg ttacgatatt atctacttcg
     2341 attgtttgag tgtcgccttt ttcagctgca ttcactaatt gttgtgcgta catgatgaat
     2401 gaatgtgagc tgtgtgtagc acctgtaaca acttctacat tagcaggtga gccatcttct
     2461 tcgcccattg ctttgacgaa ttcgtcgttc agagtaggga tgtaagtttt tggttctgta
     2521 ccgctttttg ctttcatatt ttcattatat tctgtatcat cttgttttga tttaccgtct
     2581 ttgtcgatat aatcaaagtt tgattcagtt actttgccgc cagcaactgt catttcgaat
     2641 tgcacacggt agccgttaga gtagttcttt tcttcaagct tgtaagtgcc gtctttcaaa
     2701 tcagcaccat tgtcgatttc gattgtatct gtgttaccag cttgagcagc ttggatcaat
     2761 tgttgtgcgt agttttggaa gctttcagat gagtgagtag ctccagttac gacttccaca
     2821 ccgcttgcgc tttgtgcttt taagaattgg tcattcaact caggaatgta tttttctggg
     2881 tttgtgccag attttgcttc catgtttttg ttgtattctg catcttttgt ttttgattcg
     2941 ccgttttcat tgacgttatc atattttgat tcagtgattt tgccgtcttt tacaaccatt
     3001 tcaaagactg cacggtaacc atttgaataa tttttttctt ctaatttata tgtgccgtct
     3061 tgcaaatctc cgccagctac aacttttgca gatgattctg ttgtgctttt tgcagtcgaa
     3121 gattgtgctg tagatgattc agaagaagag tttgagcttc cgttgtcatc tgcgccgcaa
     3181 gcgcctaaca ctagagctga aaatgctaat acggcaaatc cagttacaaa acgttttttc
     3241 ttcattttaa taccccacca ttccatttct agtccacaat ttttctttgt gattcattat
     3301 acattctaat ctttttagcc tataagtcaa ctatttttga taaataaccc atgtaatatt
     3361 tcaaacgttt tctttcagca aatttcgtga aaaaacgctt tattttaaaa tttgaaaaaa
     3421 atccaaaagg agttagacaa aagaacagaa catctatata atatatggag attgtgtaat
     3481 aagtatcaaa ctaaaagaat tatcatatat tcattatata gatagtctat aaggagcgga
     3541 tgatcaatgg caaagcaaaa tattgtcgtt gtaggagcag gatatgccgg tgtttcagct
     3601 acaaaatact tggcaaagaa actgaaaaaa gaagatgtag caattactct gatcgatcgt
     3661 cattcgtacc atactatgat gaccgagttg catgaagtag ctggcggacg tgtagaacca
     3721 gaagcaatcc aatacgatct gcagcatttg tttgctcgtc aaaagaatgt acaacttgtc
     3781 acagacactg taatcggtat cgataaagaa cgaaaaatcg tcaagaccaa attaggttcc
     3841 tatccttttg atcagctgat ccttggaatg ggcggagagc cgaatgactt tggtacacct
     3901 ggtgtcaaag cacacggatt caccctttgg tcatttgaag attctgtaag gattcgggaa
     3961 catattgaga agacagtagc aaaagctgca ttggaaccag atgatgaact gcgtaaagcc
     4021 atgctgactt tcgttgtctg tggttctgga tttaccggta ttgaaatgat tggggaactg
     4081 atcgactgga aaaatcgcct agccaaagat tataaattag atccaaacga aatcacactg
     4141 atggtcgtag aggcaatgcc gacgatcttg aatatgttgg atcgaaatga tgctgcaaaa
     4201 gctgaacgct atttgcgtaa gcacaacgtg gaattattat tgaatgcacc aatcgtagaa
     4261 gtggctgctg atcatatcaa attgaaagac ggacgcaaca ttccgactca cacactgatt
     4321 tggacagctg gtgtcaaagg aacttctgac gctgctgact ttggattaga atcagcacgc
     4381 ggacagcggt tgatcgccaa tgaatatatg caggcaaaag gctacgaaga taaaaacatc
     4441 tatatcattg gtgatttagt cttttatgaa gaattcccag gaacaccgac accgcagatc
     4501 gttcaagctg ctgaacaaac aggacacaca gccgcagcga atatcgtggc ttcgatcaaa
     4561 ggcggagaaa agcataaatt taaaggaaac tatcaaggat ttatggtttc tattggtgcc
     4621 aaatgggggg tagctaacct atttgataag atccatttaa gcggattttt agctatcgta
     4681 atgaaacata tcgtgaattt gaaatacttc tttgatattc gttctggtta ttatatgttc
     4741 caatatttaa tgcatgagtt tttccatatc aaagatgatc gcaacgtgac tcgaggacat
     4801 tcttctcgtt acggcaatgt cttatggagt gtgccacttc gtattttcta tggtttagtt
     4861 tggttcgttg aagcttccaa aaaaatcatt gggaatggtg aagtgttcaa accaagtact
     4921 tggtttggtg aaggatcatg gttcactgga aacagtgtgt tcccttggac atgggaatac
     4981 aatgtaacag atgttgccag tggtgcttct gctgctggtg atgcaaccac agctgccagc
     5041 ggagcagggg caagtgccgg agaagcagcg actcaagctg cacattttgg tttgagttat
     5101 gcatacggtg aagaaccaat ggctgtgatt gagaagtcac cagactggtt cacaaagatc
     5161 atgcaagtga tcatgccaaa caatgatgta gcaatgttct tccaaaaatt catggtattc
     5221 tttgaaattg ccttggcatt agcattgatt gctggattat tcacatggct atgcagtgcg
     5281 acaacgattg tattggtgat cacgttctgc ttatcaggca tgttttactg ggtaaacatt
     5341 tggttcattt ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat
     5401 cgctgggtaa ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt
     5461 tcccagtacg gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag
     5521 aaaggggctg agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg
     5581 cccgagataa gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt
     5641 tataaacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt
     5701 gaaaagtaat tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc
     5761 tcttgttata aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa
     5821 acatggaaag ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac
     5881 aacctcttgt ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg
     5941 agccttaaag aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg
     6001 cttttgactg ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca
     6061 acagcagaaa aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg
     6121 atggctggag aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg
     6181 atcgagatag atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc
     6241 gtacgtcgag gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta
     6301 gtgatcgaag tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc
     6361 acacaccatg agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt
     6421 gatcattggt ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa
     6481 actaggctta gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag
     6541 cttcttacta ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac
     6601 attcctttat agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca
     6661 attaggacca aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa
     6721 tgtcggacag cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt
     6781 gccagtcctt tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta
     6841 tcttttacga catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa
     6901 acaaaaatgg agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga
     6961 agctacatcc aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa
     7021 aacggatgga agaaactgtc cgcctgaaaa acaaacctgt cgaacaagcc atcaaagaaa
     7081 cgatccatgc cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg
     7141 gaccagagca agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata
     7201 cggccacact gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta
     7261 atatccgtag tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct
     7321 gctgttttaa actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc
     7381 gcagtatgga aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg
     7441 aactttctgt aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat
     7501 taagttgttc tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag
     7561 gagatatcgg actttctatc gggatggcct ttcagattat cgatgatatt ttagattata
     7621 caaaagagag ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt
     7681 ctttaccgtt gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga
     7741 agaaagcggc aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag
     7801 gaggcgtgga agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa
     7861 taaagaaatt acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac
     7921 tgattttgga tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga
     7981 aggaaatcta cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc
     8041 aaaaataaag agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta
     8101 ccttttcgat ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac
     8161 gcctgcgagt gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga
     8221 tttgtttcca ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga
     8281 acttgttttt gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc
     8341 tgtactatcg cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt
     8401 actgtcactt gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct
     8461 atcacttgtg gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc
     8521 acttgttgtg gtagatgtat ccgtactgtc gcttgaagct gacgtgtccg tgct
//
LOCUS       DACIRY010000112         8423 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.115, whole genome shotgun sequence.
ACCESSION   DACIRY010000112 DACIRY010000000
VERSION     DACIRY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.115"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            471..2107
                     /locus_tag="IYE22_002198"
                     /pseudo
     CDS             471..2107
                     /locus_tag="IYE22_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
     gene            complement(2252..2992)
                     /gene="truA"
                     /locus_tag="IYE22_002199"
     CDS             complement(2252..2992)
                     /gene="truA"
                     /locus_tag="IYE22_002199"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ9507392.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3183..3818)
                     /locus_tag="IYE22_002200"
     CDS             complement(3183..3818)
                     /locus_tag="IYE22_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ9507393.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="IYE22_002201"
     CDS             complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="IYE22_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ9507394.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5404..6400
                     /locus_tag="IYE22_002202"
                     /pseudo
     CDS             5404..6400
                     /locus_tag="IYE22_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6416..>8423
                     /locus_tag="IYE22_002203"
     CDS             6416..>8423
                     /locus_tag="IYE22_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ9507395.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDSFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1548 c         1292 g         2798 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggttttctt ctttatttat
     1501 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
     1561 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
     1621 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
     1681 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
     1741 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
     1801 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
     1861 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
     1921 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
     1981 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
     2041 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
     2101 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
     2161 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
     2221 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
     2281 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
     2341 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
     2401 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
     2461 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
     2521 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
     2581 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
     2641 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
     2701 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
     2761 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
     2821 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
     2881 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
     2941 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
     3001 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
     3061 agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata tatatttcag
     3121 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
     3181 ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa
     3241 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
     3301 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
     3361 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
     3421 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
     3481 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
     3541 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac aaatgcagca
     3601 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
     3661 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
     3721 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
     3781 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
     3841 cctatgcttt taattgttat tataagcgac tttatcttga tttaacagaa acctctattt
     3901 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
     3961 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
     4021 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
     4081 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
     4141 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
     4201 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
     4261 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
     4321 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
     4381 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
     4441 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
     4501 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
     4561 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagct attccagtcg
     4621 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
     4681 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
     4741 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
     4801 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
     4861 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
     4921 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
     4981 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
     5041 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
     5101 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
     5161 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
     5221 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
     5281 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
     5341 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
     5401 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
     5461 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
     5521 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
     5581 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
     5641 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
     5701 ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatac tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacagcttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaa
//
LOCUS       DACIRY010000113         8287 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.116, whole genome shotgun sequence.
ACCESSION   DACIRY010000113 DACIRY010000000
VERSION     DACIRY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.116"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(80..2200)
                     /locus_tag="IYE22_002204"
     CDS             complement(80..2200)
                     /locus_tag="IYE22_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ9507396.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2430..3608
                     /locus_tag="IYE22_002205"
     CDS             2430..3608
                     /locus_tag="IYE22_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ9507397.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3624..4382
                     /gene="aroD"
                     /locus_tag="IYE22_002206"
     CDS             3624..4382
                     /gene="aroD"
                     /locus_tag="IYE22_002206"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ9507398.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4405..4890
                     /gene="ybaK"
                     /locus_tag="IYE22_002207"
     CDS             4405..4890
                     /gene="ybaK"
                     /locus_tag="IYE22_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ9507399.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4961..6214)
                     /locus_tag="IYE22_002208"
     CDS             complement(4961..6214)
                     /locus_tag="IYE22_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9507400.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6330..7679
                     /gene="gdhA"
                     /locus_tag="IYE22_002209"
     CDS             6330..7679
                     /gene="gdhA"
                     /locus_tag="IYE22_002209"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ9507401.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7791..>8287
                     /gene="pgi"
                     /locus_tag="IYE22_002210"
     CDS             7791..>8287
                     /gene="pgi"
                     /locus_tag="IYE22_002210"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ9507402.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKK"
BASE COUNT         2652 a         1494 c         1708 g         2433 t
ORIGIN      
        1 attattctaa gatttctctt taccaacatc atttgacaaa gagcctaatt ttgtcgctgc
       61 tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga agttcttggt
      121 acgtttgcgt tttataaaga tcaacagttg attgtccctt gtgttcacta aagacactag
      181 ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt
      241 cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac
      301 cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa
      361 tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga
      421 tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa
      481 gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta
      541 cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga
      601 ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat
      661 tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc
      721 ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc
      781 cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg
      841 caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat
      901 tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt
      961 gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt
     1021 attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc
     1081 ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg
     1141 cactagtgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc
     1201 cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg
     1261 tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg
     1321 tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcaagctgg agaccattct
     1381 catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca
     1441 cattttttcc tttagcgagc ccgaacaaat catcatttgg tgctgcataa tgttctttta
     1501 cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct
     1561 ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat
     1621 aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat
     1681 ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg
     1741 ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga
     1801 tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg
     1861 ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat
     1921 aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat
     1981 aagcaactct agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa
     2041 tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa
     2101 ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacaac tagtaaagaa aaaaatccga
     2161 gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac
     2221 ttcttaatca cagaagtgtc atgaaaccat gttacacagc tcacttattc tcatttattt
     2281 tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt
     2341 ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt
     2401 gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca
     2461 atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag
     2521 aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc
     2581 taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc
     2641 aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag
     2701 atgattctac taccgctttt cgtatattca atggagaagg agacgggatc ggcggtctga
     2761 ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac
     2821 atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcgta ggaggctatg
     2881 agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg
     2941 caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag
     3001 gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt
     3061 tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg
     3121 ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa
     3181 aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta
     3241 tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg
     3301 tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt
     3361 atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt
     3421 ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc
     3481 atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga
     3541 cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata
     3601 accattagaa ggagcgatgc agaatggttt gggtaaaggg attgaatata ggaagcggta
     3661 gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag
     3721 ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag
     3781 ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac
     3841 tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc
     3901 gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata
     3961 tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa
     4021 acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc
     4081 tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc
     4141 ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttg
     4201 tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggctg
     4261 gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc
     4321 caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt
     4381 agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc
     4441 cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc
     4501 cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctca tatatttaaa
     4561 acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc
     4621 gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat
     4681 ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg
     4741 aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc
     4801 tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg
     4861 agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg
     4921 ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat
     4981 tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac
     5041 cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg
     5101 ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag
     5161 gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt
     5221 gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa
     5281 ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt
     5341 tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca
     5401 gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac
     5461 gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag
     5521 tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt
     5581 gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa
     5641 gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag
     5701 tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga
     5761 attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag
     5821 agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt
     5881 ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt
     5941 gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa
     6001 actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat
     6061 tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca
     6121 agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag
     6181 cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca
     6241 tatctgaaga aagaaaacag aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg
     6301 agggaagata agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa
     6361 ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg
     6421 aattcatgcc cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata
     6481 tcttagaact tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg
     6541 atgaaggaaa ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc
     6601 cttataaagg tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg
     6661 cttttgaaca gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg
     6721 gaagtgactt cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt
     6781 tcatgctgga attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg
     6841 gtgtaggagc tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg
     6901 atactggtgt gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag
     6961 ctactggtta tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat
     7021 ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa
     7081 aagtgcaaga gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg
     7141 atccagaagg aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt
     7201 tgacggaata cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga
     7261 cattgaaaga aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa
     7321 gcttggccga aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc
     7381 catgtacagt aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa
     7441 aagctgctaa tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgcccagc
     7501 gattgacttg ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt
     7561 accatacgtg tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag
     7621 caaatgttgc tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa
     7681 aaatgaaact ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa
     7741 aaaaaacgta aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca
     7801 ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc
     7861 aaagccaagt aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact
     7921 tccgcggctg gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag
     7981 aagcagctaa aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt
     8041 cttatttagg cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag
     8101 atagcaacac aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat
     8161 atattgctga cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa
     8221 aatctggaac aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta
     8281 agaaata
//
LOCUS       DACIRY010000114         7993 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.118, whole genome shotgun sequence.
ACCESSION   DACIRY010000114 DACIRY010000000
VERSION     DACIRY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.118"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            432..1805
                     /gene="glmU"
                     /locus_tag="IYE22_002211"
     CDS             432..1805
                     /gene="glmU"
                     /locus_tag="IYE22_002211"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ9507403.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            1899..2870
                     /locus_tag="IYE22_002212"
     CDS             1899..2870
                     /locus_tag="IYE22_002212"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ9507404.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2876..2978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3044..3613
                     /gene="thiT"
                     /locus_tag="IYE22_002213"
     CDS             3044..3613
                     /gene="thiT"
                     /locus_tag="IYE22_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ9507405.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3694..4467)
                     /locus_tag="IYE22_002214"
     CDS             complement(3694..4467)
                     /locus_tag="IYE22_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507406.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            4734..6434
                     /locus_tag="IYE22_002215"
     CDS             4734..6434
                     /locus_tag="IYE22_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ9507407.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            6404..6622
                     /locus_tag="IYE22_002216"
     CDS             6404..6622
                     /locus_tag="IYE22_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507408.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            6642..7532
                     /locus_tag="IYE22_002217"
     CDS             6642..7532
                     /locus_tag="IYE22_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ9507409.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            7550..7834
                     /locus_tag="IYE22_002218"
     CDS             7550..7834
                     /locus_tag="IYE22_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507410.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         2707 a         1374 c         1677 g         2235 t
ORIGIN      
        1 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatct agaggttgtg
       61 aaatcagcgt cctgtcctga gtattagctc aataaacgga aaaattgctc ctcatatttt
      121 gccgtttatt gagctaatac cgaaggtcag ctgattgaaa aacgtttaat gaagaggttg
      181 taagcctacc gtttaactct aagtcacaag aaacgatttc aaaaaacagc ttttcatgtt
      241 ttatgagtag tgtgacttgt gaccgaggag ttggcaggag agcaccggtt accaaataga
      301 taggcccgtt atggtctatc tattttttta tccagaaaac gctgggataa aagaaaatca
      361 cttgttttgt tgaaaaaatg ggtatactgg gataaactga aatcagcaaa ctatatttag
      421 gagtgacgca gttggacgca cgttatgcaa ttattttggc ggcagggaaa ggtacccgca
      481 tgaagtcaaa attatataaa gttttacatc ccgtttctgg taaacctatg gtagaacata
      541 tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt ggacacggag
      601 cagaacaagt taaagcacaa ctaggagaac gcagtaaata tgcgttacaa gcagaacaat
      661 tgggaacagg acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa aaaggaacaa
      721 cgttagttat cagtggcgat acaccattat tgactactga aacgctgaac aatctttttg
      781 aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca gaaaacccaa
      841 caggatatgg acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc gtggaacaaa
      901 aagatgcaac accagaagaa gctcttgtac aagaaatcaa tacaggaact tactgttttg
      961 acaacgaagc tttgttcgat gcgttaagca aagttggaac aaacaatgct caaggtgaat
     1021 actatctaac agatatcatc gaaatcttaa aagaagaagg ccatacagtt gctgcttatc
     1081 aaacggatga ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta gctaaagcaa
     1141 atgagatcat gagaaagcga attaaccaga tgcatatggt caatggcgta agctttgtcg
     1201 attcagcaac gacatacatc gatgcaggcg tagaaatcgg tccagacaca ttgatcgaag
     1261 caggtgtaca aatccaagga aatacagtca ttggttctga ttgtgtcatc gggtcacatt
     1321 caaaaatcgt agacagtagg attgaggatc acgtcgtcat tgaaaactca gtcattgaaa
     1381 gcagccatgt caagaaacat gcagatgtgg gaccttatgc acatttacga ccaaaagctg
     1441 aaattggcga aaacgtccat atcggaaact ttgtggaagt gaaaaatgct caaatcggca
     1501 aaggaactaa agtcggtcac ttgacttatg ttggtgatgc tacgctggga gaagaaatca
     1561 atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat catacgactg
     1621 tcggcgacca cagctttatt ggttctagca cgaatatcat cggacctgtc gaggtagcta
     1681 aaaacagttc gattgctgcc gggtcaacaa ttacagacaa tattccagaa tatgcgctgg
     1741 caattgcgag agcaagacaa gtcaataaag aaggatatgc aaaaaaactc ccttatctaa
     1801 actaagactt ttgcaaaatc acttgattta tttaaaaaat atgactacta tttattaggt
     1861 agaattctag agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat tactttgacc
     1921 caagattaaa aatttttgcg ttaaattcca atcgcccatt agcagagaaa attgcagcag
     1981 cagtcggcgt cgagttagga aaatcatctg ttactcaatt tagtgatgga gaaattcaag
     2041 taaacattga agaaagtatt cgtggatcgc atgtctatgt tattcaatca acgagcagcc
     2101 cagtcaatga taacttgatg gagttgctga tcatgattga cgcactgaaa cgagcaagtg
     2161 caaaaacaat caacgtagtc atgccttatt atggctatgc gcgtcaagac cgtaaagcac
     2221 gtgcacggga accaatcaca gcgaaattag tagcaaacat gattcaaaaa gctggtgcaa
     2281 caagaatgct gactttggac cttcatgcag ttcaaatcca aggtttcttt gatattccag
     2341 tcgatcattt gatgggtgcg ccacttattg ccaattattt cttagaaaaa ggaatcaaag
     2401 gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt cacacgggca cgtaaattag
     2461 cggaattttt gaaatctccg attgcgatca tagacaaacg ccgtccaaaa gcaaatgttg
     2521 cagaagtaat gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg atcgatgata
     2581 tgatcgatac agcaggtacg atcacattgg ctgccaatgc tttgaaagaa gctggtgcaa
     2641 ctagtgtgta tgcttcatgt acacatccag tattatccgg ccctgcttta caaagaatag
     2701 aagattcagc tatcgagcgt ctagttgtta cagactcgat ctatcttcca gaagatcgta
     2761 aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat gggagatgca atcaaacgta
     2821 tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa aaataaataa taaataaact
     2881 cacaaagggg agtccaattg ggctgagatt gaatctattt ctaaaccctt cgtacctgta
     2941 tcggttatgc gagcgtagga attgtgaata aacagctgtt tgactatttt cgaaagctgc
     3001 ttcctccttt gtgacttttt tattcaaagg aggaagtttt tttatgggaa aaaatagagt
     3061 ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga ttccgatcca
     3121 gatcggcagc agtttttcta tttcattagg gcagattcca ttgactcttt ttgcgttaag
     3181 aagagggaca aaggcaggga tgctggcagg tttcatttgg ggcatcctac attttccgtt
     3241 gggacaagtt tattatttaa gtgtcgttca agtgttgatt gagtacccga ttgcctatac
     3301 ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa aaaggctatg tagacgataa
     3361 tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg attggcgcac tttccagata
     3421 tttttggcat tttattgcag gagtggtttt ctggggagcg tatgcactat gggggatgaa
     3481 tccttggctc ttttctttcg taatgaatgg tgccagcggg ttgagtacag ctattgtaac
     3541 gatcatcgtt ttattaggga tccaacaaac gactccttcg atttttgtgc cagataaacg
     3601 acaggagctg taaataaaaa agaagcaaga aacttgagac agaatcatgt cctagtttct
     3661 tgtttccttt ttattttttg ataagaaata gatctacttc aatggcagat aagaaagatc
     3721 ggcatcttct accaattgcc agtgtcgatc ggtgacagag tagattaaag tagcgatgat
     3781 ttcctcttct cgataccggg tgccttttaa tttccaaata taatcttcaa tcacatagat
     3841 tgggatacga tatttaccat cgatcaaacc aacttgatca aaagaaaaaa tgatcccttc
     3901 ctgcatcaaa ttacgcattt ctttcaataa ttccacactg attttttcaa caggtgtttc
     3961 ttttttgaaa aaagattttt ttacattgat cttatctgac aagatcgaac ttaatttact
     4021 agcaaaaatc ctataaaatt catctaaata gcgataataa atccgattca gctgatcatt
     4081 aagatcgaag tatacaaaat tattctgtag tttataaaaa aacggcgaat gaagatgtgt
     4141 tttcatatga ccaaaatata ataattctga aatttctaaa ggtgtcaatt ctttcagcat
     4201 cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt tacgagaaga
     4261 ggaaaagaaa cgctgaatat tttctgtacc acggattact ttcagccctg tatgggattc
     4321 atattctcca gcagaagcag atggatcaag caagagcaag ttttgaggaa aatgcacgat
     4381 actgtttgca aagtcagtag cagtcagccc ttttgttaaa acagcattac ttgtggtatc
     4441 aatatgtaca taaactgact cagtcatcat gttcctcctc ttatttcctc ttaatctctt
     4501 ttattttaca acatttgcat taaaaaagct tgtttctttt attttaaaat tccattacaa
     4561 aatcacgttt tcattgaaaa cactctcatt ttcaattaat ttttgtgatt tttgtatttt
     4621 tttatttaaa ggaggtttta tgaattgttt tcttatggaa tctacaaagt cgaatacgag
     4681 actcatgacc tttttatgac gtactggtat acttgaagaa aaaggagagc gcgatggaga
     4741 taaaaaaagt ttacttttca cagaaaatgt cgctgtattc gcctatccag caagcaaaac
     4801 aaccccttcc attttttcaa agtcatcaag aaagtagaga cgctttttgg cgttctgcaa
     4861 aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac cgtctatttt actattgcta
     4921 aaagtagagg agatgttatt tttaataatc tgttgattga aggtgaaact tttgaatgga
     4981 aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt tattaaagaa aactgttgtt
     5041 ttttatttca atctcctcta tcagaagaat ggttgcgtat ttttcataaa aacggctatc
     5101 aaggaaccac acaattaaat aaaaagctgg tctatcatac agcacttgtt ttaggtggag
     5161 gaggagcgca cggtgcttat cagattggtg tgtggcaggc gctgaaggaa catgatatca
     5221 actttgagat tatcacaggt acctcggtag gtgcgttgaa cggcgcattg attttacaag
     5281 gagatatgaa aaaagctgtg aatttatgga aaaagctgtc gacaagacaa gtgcttgcgt
     5341 taccagaaat ggctgcagtt gaagaccttc gtgaacgctt ctatcgagaa agacgccaaa
     5401 tgacgaaaac agcactaatt gaaggcgggg tttcttctgc tccattggaa aagatggtca
     5461 aagacaattt ggatttgtcc ttactcaaac ataatcccaa aatacgttta tatacggtgt
     5521 ctactaaact gccagaacta gcggaagtag tgactgatat ccaacagaca aaaacagaag
     5581 aaataccaga ttggatactc gcctctgctt cattttatcc cgctatggct tatcgaaaga
     5641 tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa aatacctatt gatattgcga
     5701 ttaacaaagg agcaacagag gcatttgtcg tagatgtgca agggccaggc ccagcgaaaa
     5761 aaattcgtat gccagacatt tttatccatt ggaaatgtca aacattgtgg acactaggca
     5821 gctttctgct ttttgatagc caaaggaacc aattaaattt acaattaggc tatctggaaa
     5881 tgaaaaaacg attaggctat tactatggga actggtatac atttcattct gttaaacctg
     5941 caagaacata ctggcgagga tttttgtctt acttaacaaa agaaattcag ttagaattat
     6001 cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa tttatataag aacagggtcg
     6061 taccagagac atgcggattg gcgatgctag aacttttagc aaaaaaacat tttttactgc
     6121 ccaatgaaat ctatcaagta gacatgatga ttcaaatgat ctgccaacaa gatataaaaa
     6181 gtatgccgga tgatttattg gcacaaattg gtcaattaag tactgaagaa tggcgaaggt
     6241 atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa aactgtttat tttgattatc
     6301 tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt gcttaagcaa ccagttgaca
     6361 cattactgat cctatattta tattatctca aggaggaaca accatggcac aagaattttc
     6421 atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac aacaaggggt ggcgtaaaga
     6481 actgaaccta gtcagctgga atggtcgccc gccaaaattc gatcttcgag attgggcgcc
     6541 agaccatgaa aaaatgggga aaggcctgac attgacgaac gaagagtttg aacaactgca
     6601 aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg aatgaaaagt atgactggtt
     6661 ttggcaaagc catccgcgaa acaaaagcgt atcagttaga tattgaaata aaaagtgtca
     6721 accagcgttt tctagatgtt caaatacgaa gtcctaaaat cctgaacttt gttgaaaacg
     6781 attttcgtca gcttattaaa cagcagttaa gccgcggtcg aatcgaagta tttgtgaatc
     6841 tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa ctgggaattg attgattcac
     6901 ttatcactca attgacagac ggaattcaac aacgatacgg tagtcaaaat caagtgtcaa
     6961 ttagtcggat ggcggaacgc ttcgcgctgg acgaaaactt cgtaacggta gaagaaaaac
     7021 cggaagagag catggaagaa ttggcacagt tagctctaga tacattgaaa agcgcacttg
     7081 attctattga ggcgagtcga gaaaaagaag gtcaagcttt agctttagtg ttgaaaaaga
     7141 atactgaaga attcaaagaa gtactaaaaa aactcgatag ttttgtagaa atctatgaaa
     7201 aggaatttca aataaggtat caaaaaaagc tggaggaatt tcttggagcg aaagtcgatg
     7261 agcaacggtt gttgacggag ctagccatct tgttggaacg cggagatatt cacgaggaac
     7321 tggatcgtct ggcgatccat gtacaaaaac tagatgaact tatcgaagca caatgtcctg
     7381 taggtcggga attagatttc ctgatccaag aaatgaatcg ggaaatcaac acgatcggtt
     7441 ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca gtctaagacg atcttagaaa
     7501 aaatcagaga acaagtacaa aatattgaat gacgaaagag gtagagataa tgaaatatta
     7561 tgacgtaacg ttccacgaat taagtggaaa aactgtggtt aaacgggata tcccttctga
     7621 aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac tataatgaga acgaactgtt
     7681 tattttgatc aacgacggtg cttacgtgac gatgaatcgg aaatttattg ttcgaattga
     7741 cacagaagag gtagaagatc cgacagaaaa agcacgttct cgaaaagatg agatcatggg
     7801 tgtagtgaat actttgtcca atatgggctt ttagcaagga tgctgaacaa agaaatacaa
     7861 taaataatca gaattggatt tcttcgctta gcttctttgc tctaggaata catttaaagg
     7921 ttgtgacaaa attcgtgtca caatcttttt ttattttgaa taagtgatgt tcaaaaacca
     7981 ttttctcggt att
//
LOCUS       DACIRY010000115         7981 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.119, whole genome shotgun sequence.
ACCESSION   DACIRY010000115 DACIRY010000000
VERSION     DACIRY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.119"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1554)
                     /locus_tag="IYE22_002219"
     CDS             complement(<1..1554)
                     /locus_tag="IYE22_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9507411.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNK"
     gene            complement(1610..4090)
                     /locus_tag="IYE22_002220"
     CDS             complement(1610..4090)
                     /locus_tag="IYE22_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507412.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(4074..4409)
                     /locus_tag="IYE22_002221"
     CDS             complement(4074..4409)
                     /locus_tag="IYE22_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507413.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(4423..5457)
                     /locus_tag="IYE22_002222"
     CDS             complement(4423..5457)
                     /locus_tag="IYE22_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ9507414.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(5547..6227)
                     /locus_tag="IYE22_002223"
     CDS             complement(5547..6227)
                     /locus_tag="IYE22_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ9507415.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(6511..7614)
                     /locus_tag="IYE22_002224"
     CDS             complement(6511..7614)
                     /locus_tag="IYE22_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ9507416.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         2317 a         1642 c         1414 g         2608 t
ORIGIN      
        1 tttattcatc cttaaattca atttttctaa ataacattct tttaaaaatt ggttcaaata
       61 ggggaaatca taattatatt cgttgctagt gaatacaaaa actttagcgg acttctgtga
      121 cgggtactct ttcaattggc tagtcaaaat gatatcatac tgcttttctt tctcgtattc
      181 gactaaatca tatttgatcg tattcagcaa ttgttcctgg atgatttctt tcacgatatc
      241 cgccattagc ctttcataag gaagatcata agcaatttgc agacgtaatt ctaactgctt
      301 gatcagcgtc cgcaaaatag atgcatagcg agcaaataat tcaaagcggg cattttcctc
      361 gtctacttca gtaaaatcaa caatagcaaa tgtttgtctg accaatcttt cacataaaac
      421 caggaattgt ttatcttcta cttcagttaa ccgctgtttt aatccttctt ggccaaaaat
      481 attcatccat cctctaaaat aggcaaaatg agcgtgattt tgcatcactg tcaacgcaat
      541 cttttgcgaa atctcttctg gaaaaacata aaaagagaaa tattcttcta tcgattgttt
      601 aaacgttcga ttcaattgag ctactacgtc gtctttgtat ttttcgctta tcaaaaactc
      661 ctggacatgc gagttatgtg tatctaatgt aaagttactg atcatgaaaa gataaaaaac
      721 caacgtttca ttttgattcc aaggaaaggc atattgaaag aagtaacggg tcaaaaatgt
      781 atggaggtcc aataaaaatt gtgtcgtttg aaacaatcca acaggaagat tgctataatc
      841 tggtttggat tttttcgtac agcgcattcg cttcttcata atggaaagcc atatttcaaa
      901 tttcaatcgt ccttcttctg taatagtaaa actaaactct ttttgcagca gatctaaaaa
      961 agtgtttcct gataaatcac tgtattcttt tttgttttca agataaggac gtccaaacca
     1021 aaataattgg aaataaaaat aacaaatttg actttctgga ccggtcagcc gcccgctttt
     1081 tattttcaga tgaaactcat ttaaagaatc atttaattcc ttcactctgc gagacaaagt
     1141 agccgaactg atattaagtt ccatgacaag tttctgccga tcaaatgagc cattttccaa
     1201 cagataaaga agtatctgat attttgtaga atagcgcaag tagcaatgat aataaacatc
     1261 gactgctgct atatttccat gtctgattaa taaaatattc ccttcttgga taacaatttc
     1321 caattgtttg atattttcca agttagcttg taaataaaac agatcttctt tcaaggtagc
     1381 ccgatcaact gataagcggg taagtacgtt attaacggaa atctctcctt tttcctctaa
     1441 taaaagttgg aaaagtttga tttgccgttg ttcatttttt tcaagcaaac tgaaaagttc
     1501 caagtccact cctccaagca tttctgattt taacagcaga agtttccttc tcatccttga
     1561 aaaagaggat ctgtgacagt tatcacagat ccatacaaat tctaatcact tagctggttg
     1621 gcccagctgt cagttcccat gtgaccatgc cttgatacgt tgcgccaggt tgagcattcg
     1681 tactgctatt tacctctaag agtaaaccat cggtatcggg aaagtcaacg agaacctctc
     1741 ctttagctgt tccatcactc tcataaatca acgtgttttg accttgctga atcaattgtt
     1801 ttgatccaga atgattgaag tataattttg ttccgcttaa ttcttgacct tgatcattct
     1861 taaataaaga agtcaatgtc cctgtcagtt gccaaggaga cgtgcttgga agccgagtat
     1921 cccgcacatc aaaagaaaaa tcgccttttg cttcataata acttgtcttc aacggtttgg
     1981 ggagatcgtt accaaatgta aaatcatctg gtacattaac taatgttaat tcttgtttgg
     2041 ggacaagaat tgttttagca gatgtttgga caactgggtt aggaagctta gcaccatctg
     2101 ctaatgtaat attgctggaa agtacagctg cctttgtcag tatgctgacg gattgtacag
     2161 aagtatctac agtatttaat tgatagttgt aagtaagatg aatggtatct cctattttgc
     2221 tcaatagatc tgctgggaca gaaactcctt gttgaatgcc ggtatctgcg atagttgtac
     2281 catttaattt taaggaacca ttgatataag tagtattttc aggaagtgag atcgtgattt
     2341 ttttgtcggt tatcgcatcc ccagattttg ttaacgtgaa tgtcacattt tcttgaatcg
     2401 tatctcctga tagagcagac aggggatctc ctgtctcagg ttgtctgcta aggtttttta
     2461 gctgactatc tgcactcaca gatggaaaat tagccactgt tatttgagga agagtggtga
     2521 ttccagcttg ttcagactgc catttgtacg caaaagacgg atacatgtta gtaagtacat
     2581 cgtccaccaa gcgataatca aaataatatt gccctgaagc caatgcaggt agtgtagaag
     2641 tagctgtatt tacacccccg ttatttgatt ggatcgtttg gaaaagttga tctgtccatc
     2701 ctgtcgctaa tttttgggtt cctttcgaat aaagcctgta atggatcgaa ccctttttta
     2761 gttcagttgc tgaccaattg gccgtgactt ggtacgatgc agaagaaagg agctttcccc
     2821 actgactatc ggctgttaag tttgttggta aaaccgttcc gtctagcttg tactgctcca
     2881 atacctttaa agcaacagga gaaagcaatc cgccttgcgc atctttccca ctgtacacga
     2941 tattggctgt ctgattcaat ccacttgtgc ttgctggagt atgagtcagt attgctcctt
     3001 gtgtaacgtt ttttcctgta aaatagttga ctacgcgatc aaagcgacta aatggcaact
     3061 gatcaaaacg tccttcagtc agccccatcg tcaatgtccg actattggta gagcctggaa
     3121 ggttctggtt atttccatcc acgtattctg tggtatcagc aaatgtatta tattttcctg
     3181 ttgaatcact tgttgagatg taactattcc acgtatttat ctctgcactt gcagaaaatg
     3241 catatgctcc tggattcaag tttccgccag ttaatttagg ataaaatcta cctgcagctg
     3301 gagctaaaat cgaattgaac atacgatctt ggataatatt attcgaatca taaaaatcaa
     3361 cagaaaaaac ggccttttta gacatagaag gttgtaattt acccttggtc aaaattttgt
     3421 ttggactcgt atcatattta tctccgtttt ctgtcatctt cgcgacatta gctgtaacat
     3481 caaattgaag cgggatatcg tccacgttga attcgcctcg cgtaataata gcaataatat
     3541 ctgccaatag agtatttgta tcccacgacc ccatcccaaa acgcaaggta tggcttcctg
     3601 ctgataataa actaccgcta gtaaccttga tgggggcacc ggcaatatac aagtaggcgt
     3661 tgttccagtc aattgctttt aatacatcac tgcttgatac atgttccggc aaattgatga
     3721 tcgccacaaa tgcaaatcct tgtgcagtga tattcaccaa atcaagtaca tttgttagtt
     3781 tcacatggct ttcccaatga aaacggaccg tgccaccttt ccaaggatca tttgaaccat
     3841 cgttcaaata agtagcagtc aatttaggca attggttagc cccagttagt tggacaagct
     3901 tcccgccatt gactgcacta cgtccaccat ttggaggtac gatattttca tacgtgtatg
     3961 gatcataaga aatggcagga ggcgttgctc caacagcttc tgccgaaggt gaaaagataa
     4021 atatacttaa aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa aatttatttt
     4081 gtagcaacat gttcatcctc cttttccttt cttctccata acaacaacca cgcaagaagc
     4141 cataattcta taccaatgat caaataaaga aaacggatct tttcttcatt cgtctccggg
     4201 aatacgccat gttgattaga ttctttataa atcggtagct gtgtctgctg ttttgatgcg
     4261 ccattgactg aaccacttgg atgcggaact ggacttagat cataaaatgt aatcgtacct
     4321 gaacttttgg tatctgcttg gactggctct tgccagctga agaacagcaa acttgcaata
     4381 atccctccca ataagatcga tgttttcaac gaaatcacct catcatgttg tttctccttt
     4441 tttctttttc ttgtggaccc acaaaaacag caaaatcaac cataacacta gaactgcagc
     4501 aaccaatcct gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa cggaattttc
     4561 atttactttt tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc gccattcatt
     4621 actgtcgcct gattttgcta tgatttctgc gcggtaatcg ccggcttttg ctttatcacc
     4681 agctaaatca agattcaagt gaaaattcga atttggtgca actaaaaatt cattagacgt
     4741 attttcaata taaagctgat tttcataaaa gatcttgctc gtaaatatca aattgctgat
     4801 aatcctaggc tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt tgacctcgtc
     4861 caaatctcct gataaaagtt tcagatccgg ttctatcgtc tcttgacttt cttgtaaaac
     4921 gactgcgaga ttataagcaa tacgattttt gatttgtgct ccttctgtag cattttctgt
     4981 ttcagcttct tctttaggtg aaacatgaat acccgctaat acacgtccct taaagctttt
     5041 tgctgggatt ttcacttcta taggtatagt tgtttctgca tgtgctgata cattgatttc
     5101 ctttttcgca atgagcaaaa cgtcacggac atcaaacggc aatgtctcat ctaacgttgg
     5161 gtttttttga ctataatcga tcgttccacc atcactcgtt accgccggat tgacagagac
     5221 ctcaaatgtt cgttcttcat ctgaagaatt gctgataatt acttctagtg tctgattttg
     5281 ttccggtgcc actaataaat caaaataacc gattgcttta tctatttggt tctctggaaa
     5341 taccggtttg attccaaagt ctcctgctga atcctttgct tggctgattg acggaaaaat
     5401 gaaaaataga cttaaaatag aaaaaaacga aacaaacagt cgtttacttt gattcattgt
     5461 ccaccacatc ctttcaacaa caactgcggt aaaaaatcct tcacctatag tattttcacc
     5521 gcagtttgtt gaatttattt catactctat gctactgggc cagccacgag gttccaagtc
     5581 aaatcagccg tgaatgttcc agctgctgct ttcggcactt tcagtgtgat atttgctgga
     5641 tcataaacag aaagccatgt tcccagacct tgatcttttg ctgctgtaaa tattggtgtc
     5701 gctgcacttg ctttactcaa cccagttaca gtttgactag ttggtccagt ggattcgtta
     5761 tctgacgtac ttttaacagt ggatgttcca aaatctaatt cagcgccttg caaaacttgt
     5821 gaaccgttta caaaatctga aaccgaaact gtcacgttcc acccttgtgc ttggccatct
     5881 gcacctacac ctcgacgatc tgatacttgg agataaggtg tatcgttttt tgaagcgtct
     5941 acttgatacg tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa ttcaggaact
     6001 acatccaatg ttaaagaacc tgtctggcca gttgctggat tttccggatc tgctggctct
     6061 actggttttg aaggatcagt agggttaaga ggctcaacgg tatttgttgg ttcagtaaag
     6121 gtaatatctc cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc caagccgcct
     6181 agaagaagtg ttgaaagtaa taatgtccca atcatgttct ttttcataat aaatcactcc
     6241 tttttcttag tgacctaagt atagtagaga cagtatgcaa aaaatcctca tgaattcatc
     6301 cgcttacttg aaattatgac cctttttgta taaaagagaa aaaaacaata aaatcgttta
     6361 catgaatatt cttccttttt tattaagtat gaaaaaatat ttcaatttta ttttttcgtt
     6421 tcaaattatt ctattagtga catccttgaa ctataaaaat aggagtacgg catcatctgc
     6481 cgcactccta tttttttcgt catttatttc ttaaataaaa cgattctcac gcaattttat
     6541 ttttttgata tcttctactg tttgtgttcc agctaattgc atgaccattt ccaattctgt
     6601 tttaaagaaa tcaaacactt gatggactcc tgtacttcct cctaaagaca atccataaat
     6661 aacgggtctg ccaatagcaa caagatcggc acctgatgcg attgctttga aaacatgttg
     6721 tccacgtcgt acaccgctgt caaacacgat tggtacacgt ttatctactg cttctgccac
     6781 atattgcaac gaatcaaacg cagcaggccc cccatctaat tgacggccac catggttaga
     6841 tacccaaatt ccaccagcac cagattctaa tgatcgatag acatcttctt ctgattgaac
     6901 acctttcacg tagactggca aatcagaatg agcagcgata aattctacat cttttggact
     6961 aagtttttgt ttggaagatt tatatacagc atccatcgtt tggcctactc ctgattgata
     7021 agcctgaacg atcggcattg gcagcgggaa ggtaaacccg tttctgcggt ctgtttcacg
     7081 gtttccgcca actgttgcat ccgctgtcaa tacgattgct tttgctccat tacgcttggc
     7141 cacttcaaga atatccaaat tgattccatt atctttgctc atataaaatt gaaaccattg
     7201 cggtgctttc tccccgcctg cttctcttat ctcttctaaa gtacaagaag cgtaagaact
     7261 tgctgtatag atcgtcccaa aatctgctac accttttgca gaagctttct ccgccttcac
     7321 atgagctaat ccatgagcag caactggtgc catgatgatt ggtgcagtca gcatttcttc
     7381 gtcaaaatgt gtagttgtat cgggtaattc tacatctctt aatacatgag gaataatcaa
     7441 acgatgattg aatgcccgtt cattttcttg ataagtaaat aagtcgccag caccactgct
     7501 gatgtaacca taaccaccag ctggaatcac ttgtgaagcc gcgatttcta aatcttccat
     7561 attgataaaa tctactattc cttcatttgt acctgcttga tatgtttttt ccatctcaat
     7621 gccttctctc tataaaattt gaatgatgaa caatatgata gcgtttacac tttttatatt
     7681 actcttcgac attgatacag gcaaataatc tgtttacatt tcagccattc cagatgtcac
     7741 tactctggaa gaaatgattg taataaaaat agataaaaaa ccgatgggac tttgacaaaa
     7801 gtagtgtcaa agtccctttt tttgaataaa ctgtgttcaa aagccatttt ctcggtatta
     7861 agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     7921 actaagcgac tttttcacaa cctctcgata aaacggtgtt caatcagctg accctcggta
     7981 t
//
LOCUS       DACIRY010000116         7979 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.120, whole genome shotgun sequence.
ACCESSION   DACIRY010000116 DACIRY010000000
VERSION     DACIRY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.120"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IYE22_002225"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IYE22_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ9507417.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IYE22_002226"
     CDS             1228..2331
                     /locus_tag="IYE22_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9507418.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IYE22_002227"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IYE22_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ9507419.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="IYE22_002228"
     CDS             complement(3821..6268)
                     /locus_tag="IYE22_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ9507420.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="IYE22_002229"
     CDS             complement(6457..7893)
                     /locus_tag="IYE22_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9507421.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2214 a         1736 c         1358 g         2671 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa ttatgtaaa
//
LOCUS       DACIRY010000117         7892 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.122, whole genome shotgun sequence.
ACCESSION   DACIRY010000117 DACIRY010000000
VERSION     DACIRY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.122"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1041
                     /locus_tag="IYE22_002230"
     CDS             <1..1041
                     /locus_tag="IYE22_002230"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ9507422.1"
                     /translation="NWKYKSDEERKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQIVE
                     IIKNEKIDIIHCNTPIGGVVGRIAGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPME
                     RWLAHYTDVLITINHEDYTLAQKLRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESI
                     CKELNIPDTAKIILSVGELNENKNHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNI
                     ANELGLHQRVFLLGYRSDIPKLLAAANIYILPSLREGLNASLMEAMASGLPCIVGNIR
                     GNVDLIEDGKGGFLINPKDEEEIAGAINRLADNKLLCESMGAHNLDKIKDFDLSVVIN
                     QINNVYHEIVSK"
     gene            1064..2116
                     /locus_tag="IYE22_002231"
     CDS             1064..2116
                     /locus_tag="IYE22_002231"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9507423.1"
                     /translation="MKKIKIMHLLQSNRFSGAENIVCQIMSLFQNNEKFEMIYVSPDG
                     PIANILNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSH
                     LHNNPLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNT
                     ERIIKMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLF
                     DEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPV
                     VAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNMN
                     RYQGVLEKIYVSAALK"
     gene            2645..3421
                     /locus_tag="IYE22_002232"
     CDS             2645..3421
                     /locus_tag="IYE22_002232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ9507424.1"
                     /translation="MAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGL
                     YLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLFN
                     INRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYLI
                     GYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKWL
                     FLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
     gene            3432..3893
                     /locus_tag="IYE22_002233"
     CDS             3432..3893
                     /locus_tag="IYE22_002233"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ9507425.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            3952..5409
                     /locus_tag="IYE22_002234"
     CDS             3952..5409
                     /locus_tag="IYE22_002234"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAQ9507426.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLL
                     SVCLCVILYFFIYSCIFKGKTEIINMVRMFTGKGY"
     gene            5444..6613
                     /locus_tag="IYE22_002235"
     CDS             5444..6613
                     /locus_tag="IYE22_002235"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9507427.1"
                     /translation="MKKVTILIPGMYYGGMERVAFITRQILLKNNYNVSFVTLFDNDA
                     DYQPNFEYLSLKCDIKPSKFGKVINIFKRVYKMKSVKKKLKTDITISFGQSCNLVNVL
                     SKRDDKVYVGIRSYDWLFQYIFHYYVDKFVYKKADAVVSVSQLIKRDAENIFNFNKDK
                     SYFLYNPYDLELINNKSKEKITEIEVPTDKKIIVTVGRLEDQKGLYHLIKAISMLKNK
                     DNVVVYILGHGTKKELLENLIKAYQLQNVIFLLGGQSNPYKYMSRADLYVMPSITEGF
                     PNALVEAMSTETAVLCADCKSGPREILSNRTLDTICENIEYAESGILVCPMTDSRNYS
                     PQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
     gene            6651..7742
                     /locus_tag="IYE22_002236"
     CDS             6651..7742
                     /locus_tag="IYE22_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ9507428.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(7810..>7892)
                     /locus_tag="IYE22_002237"
     CDS             complement(7810..>7892)
                     /locus_tag="IYE22_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAQ9507429.1"
                     /translation="IIANRYDSNLDDVKEKVYTRDLFQRD"
BASE COUNT         2806 a          962 c         1425 g         2699 t
ORIGIN      
        1 aactggaaat acaaatccga tgaggaaaga aaagctgatg aagtgaaata tggaataaaa
       61 atataccaag ttgattttta tagaaaccca ctaaatttaa aaaatataaa agcttatcat
      121 cagattgtag agattataaa aaatgaaaaa atagatatta tccattgtaa tacacctatt
      181 ggaggtgttg tcggacggat tgctggtctt aaatgtaaag tcaaaaaagt aatttaccag
      241 gcacatggat ttcattttta ttcaggtgct cctaaaataa attggtttat atattatcct
      301 atggaacgtt ggctagctca ttatacagat gttttgatta caataaatca tgaggattat
      361 actttagctc aaaaacttcg tcttaggaaa ggtggaaaag cttattatat tccaggtgtg
      421 ggtatagata caaataggtt tatgtcttcc acatcaataa ggaattttag agaaagtata
      481 tgtaaagaac ttaatatacc tgatacagca aaaatcattt tatctgtagg agagctaaat
      541 gagaataaaa accatttatc agtaatacat gcattacaaa aaattccgct gcatacagat
      601 atctattata ttatcgctgg ggtaggagaa atgagggaga cactacaaaa tattgctaat
      661 gaattaggat tacatcagcg agttttttta ctaggatata gaagtgatat acccaaatta
      721 ttagctgctg caaacatata tattctaccc tcattaaggg aaggcctcaa cgcctcttta
      781 atggaagcga tggctagtgg tttaccatgt attgtcggta atataagagg aaatgtagat
      841 cttatagaag atggtaaggg tggatttcta atcaatccta aagacgaaga agaaattgct
      901 ggtgcgataa atagattggc tgataacaaa ttattgtgtg aatctatggg ggcacataat
      961 ttagacaaga taaaagattt tgatttatca gttgtgataa accaaataaa taatgtttac
     1021 catgaaattg taagtaaata attgaattga aagtgatatt aatatgaaaa aaataaaaat
     1081 tatgcatttg ttgcaaagta atagattttc tggcgctgaa aatattgttt gtcaaataat
     1141 gagtttattt caaaataacg aaaaatttga gatgatttac gttagcccag atggtcctat
     1201 agcaaatatt ttaaatatta gaggtttaag ttacttacca ttaaaaaaat tttcgagaaa
     1261 agaaattgac agagccgtac atgaattaaa cccagatata attcatgctc atgattttaa
     1321 tgctagtgta agagcaagta gatataagaa gcaaatcatc ctttcccatt tgcacaataa
     1381 tcccttgtgg ttacctaaac tagacataag aactattata tattctttat gttcgtctag
     1441 gtttaattca gtaattggtg tttcgaattc cgttaaggaa gaatttcgtt acaaacaatt
     1501 aataaaagat aaatttgtgt tgttacctaa cactgtaaat actgaaagaa ttataaaaat
     1561 ggggaatgaa tttgttgaag atgatattga tattttatat gtaggacgca tggcagaacc
     1621 taaaaatcca ataggtgcaa ttaatgtttt agaaaatgta gtaaaaaaat attcaaaaca
     1681 tttacgtgtc gtgatgattg gtgaaggacc attatttgat gaagttaaga tgcaaataca
     1741 aaaaagaaac ttaacagatg ttattgagtt aaagggattc aaaagtaatc cctataagta
     1801 catgaagaaa tctaaagttc tattaatgcc atctctttgg gaagggtttg gcctagtatc
     1861 agtagaggca atgttgttag agacgcctgt tgtagcttat aacgtaggag gattgaagga
     1921 tattatcgat gagacaaatg gatttctgtg tggtagtgaa gttgaattat ctaattcact
     1981 aatgaagcta ttaaattcag aagaacttag aaaaaaaatg ggagaaaatg ctgctattag
     2041 tgcaaagaaa ttttctaata tgaatcgtta tcaaggagta ttagaaaaaa tttatgtatc
     2101 tgcagcttta aaataaaata tgaattataa aaatagagat caattagttt tacgaaaaat
     2161 aatacccatt agtattttgt ttcaatcagg aatgattttt tattcactga tgccctcaat
     2221 agcaattgca gttgtttctt tttttatgtt tttgtattat atgtggtgct taaaaaagta
     2281 tttttgttta gcagatagct tattgttaac atcaattctt ttcattccaa catcaactat
     2341 atctatatta ggaactagtt attctagctt accattgacg tggcataatc taataataat
     2401 aattttgatg ttatatatta ttatagaagg aacattaaat cgttcatact tgattatttt
     2461 atttttattt atttgttttg gaatatcgac gtctatgttc ttaccttttt taatagatgc
     2521 attaaagcaa ttaatgacta tttcgttgtt tatctgtagt tttttaatag gaaaacgttt
     2581 taaaatgtat agcaaaaact taactatgat tgggtggaat ttctatttca taggagtagt
     2641 atctatggcg tttcaggtat atttgcaacg agtatatatt ttatctactg gtgtaataat
     2701 aggccattat gcagaaatgg gtttaaatag aattgcatat gcaggtttga tgggagattt
     2761 tagttttgca gggttatatc ttgctacagg ctgtctatat gcttttctaa aatatgtgga
     2821 tttaaaaaat tggaaattat tattctttat aataatagaa gtatttttat taggtgcaat
     2881 tttatgtgtt agtgcaagaa cagggttagt ttccttgttt gtcacaatga cattgtatta
     2941 tctatttaat ataaatagaa ttagtgttcc tcatctttta gttctaatag gtggaatcat
     3001 aactacacct tatatactag taatgttaat ggcaggtcgt tctggacaaa gtcttttgga
     3061 ttcttcaggc agattagaaa actatatgtc ctctttaaat gcttttagtg ataaatactt
     3121 aataggatat ggattaggat taaataattt atacacgcta ctaggtgtag cagtaccaca
     3181 taattttttt attcaatatt tattacagac aggaattatt ggtacgctaa ttattttaag
     3241 tggcttcttt aaatttacgt atgaagaatt aaagaaatca ggttgctata aatggttatt
     3301 tttgatgata gtagtgggag caatgtttat accggatatt gtatcttcgc gttttcttta
     3361 cgcaatagtt gttttgtgta tgatttctgc atcagaaaga actataaaaa aagaaagttg
     3421 aggtagaaaa aatgagaaga aaagcagaga aatgggcaga taaagcttgt agacaataca
     3481 ggaatggtca aaataaaatg tcaaaaatat cctgctatat tttaaggatt atttatggat
     3541 gtgaattcta cggacaggta gattttagct ctaatgctca actagtccat aatggtcttg
     3601 gttgtgtatt ccaccctaaa acaaaaatcg gtaataattg taaaatatat caaaatgtga
     3661 ctttgggtgg aaatggcaaa ataataaatg gaaaagaaac tgttggggca ccaacgctag
     3721 aggaaaatgt ggctgttttc gctggtgcat gtgttttagg accaattaat atcggacatg
     3781 attcaatcat aggtgcaaat gctgtagtta caaaaagtgt tccaccatat agcttagtat
     3841 atggaaatcc agcaatcata acagagttga aatttgatta taaatttgat taaaaagtaa
     3901 tgtttaatat gcgaaaggtt gctatattta tttagcaaaa tggggtaata aatgggaaat
     3961 aatattcatg gaaagacagc aaaagcttta aaatggtcat ccttagctga aataattgca
     4021 aagattataa ctccaatttc gaatatgatt ttagctagat tattgactcc agatgctttt
     4081 ggagtagtag ccactgtaac tatggttatt agtttcgctg atatgtttag tgattcagga
     4141 tttcaaaaat ttttagtaca gcatgaattt gataatgaag aacacatgtt ttcatcaaca
     4201 aatgtagctt ttactagtaa cttaggtatt tctttattat tatggttatt tatttgtgtg
     4261 tttaataata aaatagcgca cttagttgga aattctggac ttggattagt aattatagta
     4321 gctggagttt ctcttccaat tacttctttt tccagtatac aatcggcaat ttttagacga
     4381 aattttgatt acaaaatttt gtgttatgct agaataatat ctgcattgat acctttagtt
     4441 attacagtac cgttggcgat aattggacta agttattggt cactagtaat tggatctata
     4501 ataggaaatt tagttacagc tgcaatatta actaagttat cagagtggaa accaaaactt
     4561 tttttttcaa aaaaagaatt ttacgaaatg ttttccttta gtgcgtggtc actagtggaa
     4621 tcattaggta catggttgtc atcttatata ggtactttta tcgtaggagg cattttaagc
     4681 gtatactatt tgggaatata caaaactaca atgacaacgg ttaatggtat ttttgcaatt
     4741 gtaacttcag caacaacaac tgtattattt tcaacattat ctcgtttaca aaatagtaaa
     4801 acggagtatg ataagacata tttagatttt attaaaattg tttccatttt tattattcct
     4861 ctgggtatag gtatatatgt atatagggaa ctagtaacaa atattctact cgggagtcaa
     4921 tggaaagagg ctgtaccatt tgttgggata tatggattaa tgagctgttt tactcttgtc
     4981 ttgggacaat atgcctctga gtactatcgt ggtttaggaa aaccaaaagc aaatgttctt
     5041 atgactatcc tacatttagc agtattaatt ccagttctaa taatctattc aaggaaaggg
     5101 tttatagaac tagcatatgg tagatctttc gttaagatag agcagattat agtcttttgg
     5161 attattttat ggtttggttt taagtttaat ccatttaaaa ttttaaagca ggtttggaaa
     5221 tcttgtgcta gtgcattgct aatgggagtt ctagcaaaaa ttatgttgga attctgtact
     5281 aattatgtta tgcaattact ttcagtgtgt ctttgtgtaa ttctatattt ttttatatat
     5341 agttgtatat ttaaaggaaa aacagaaata attaatatgg ttagaatgtt tactggtaaa
     5401 ggatattaag agagattttt atggcaaggt tgtgattagg aaaatgaaaa aagtaacaat
     5461 tttaattcct ggaatgtatt atggtggaat ggaaagagta gcttttataa cgcgtcaaat
     5521 acttttgaaa aataattata atgtatcatt tgtaactctt tttgataacg atgcagatta
     5581 tcagccaaat tttgaatatt tatcattaaa atgtgatata aagccatcaa aatttggtaa
     5641 agtaattaac atttttaaaa gagtttataa aatgaaaagt gtcaaaaaaa aattgaaaac
     5701 ggatataact atatcttttg gacaaagttg taatttagta aatgttttat caaagagaga
     5761 tgataaagta tatgttggaa tccggtccta tgactggcta tttcaatata tattccatta
     5821 ctatgtagat aaatttgtat ataagaaagc agatgctgtt gtgtctgtca gtcagttaat
     5881 taaacgtgat gctgaaaata ttttcaattt taataaggat aaaagctatt ttttatataa
     5941 tccatatgat ttagagttga taaataataa gagtaaagaa aaaattacag aaatagaggt
     6001 tccaacagac aaaaaaatta tagtgacggt tggtcgatta gaagatcaaa aaggtttata
     6061 tcatctaatt aaagctatat ctatgttaaa gaacaaagat aatgttgttg tgtacatctt
     6121 aggacatggg acaaaaaaag aattattaga aaatttaata aaagcatatc aactacaaaa
     6181 cgtaatattt ttgctaggtg gccaatcaaa tccttataaa tacatgtcac gtgcagattt
     6241 atatgtcatg ccatctataa ctgaaggttt tcctaatgca ttagttgaag caatgtctac
     6301 agaaactgct gtcttatgtg cagattgcaa atctgggcca agagaaatat tatcgaatag
     6361 aacgctagac acgatttgtg aaaatataga atatgctgaa tcaggcatat tagtatgtcc
     6421 aatgacagac agtagaaatt attcgcccca atatgaagaa tgtgataaaa atttagcgaa
     6481 tgctatagaa gaattgattg aaaacgataa tttgaggaat aagtatgcaa aagcagcaaa
     6541 aaagagagca gaactatata gttatgatgc ctttgaacat aacttgttgc aaattatgaa
     6601 gacatttaat taattcatat gatgaaataa aaataaggga gtataactag atgctgaaaa
     6661 aaaatacatc tttacagaat aaaacaatat taataactgg cgcagccggt tttattggat
     6721 caaatttggt tcttgagtta ttacgtactc agtcatcaat aaaaatcatt ggcattgaca
     6781 atatgaatga ttactatgat gtctctatta agaactatag attatcagag atagaaacgg
     6841 aggcaaagaa ccatccagga tctacttgga attttatcaa aggttctatt gctgataaag
     6901 caacaattga tttcctcttt gataattata aaccaagtat cgtggtgaat ttagcagctc
     6961 aggcaggagt ccgttattca attactaatc cagatatgta tatcgaatct aacataatag
     7021 gattctataa tattcttgaa gcttgtcgtc attcatatga taatggagaa tctggtgtgg
     7081 aacatttagt gtatgcctca tcgtcttccg tttatggtac taataagaaa attccttatt
     7141 ctacagagga taaagtagac aatcaagtat cgttgtatgc agcaactaag aaaagtaatg
     7201 aatcaatggc acatgcatat tctaaacttt acaatattcc gtctacaggg ctacgtttct
     7261 ttacagtata tggtcctgct ggtcgtccag atatggctta ttttggtttt actgataaac
     7321 ttcgtgctgg tgataatatc caaatattca attatgggaa ttgcaagcgt gactttacct
     7381 atatagatga tattgttgaa ggagtaaaga gggttatgca gtgcgctcca gagaaaattg
     7441 atggggaaga tgggctacca atcccaccat atcatattta taatattggt aacaatacgc
     7501 cggaaaacct actagatttt gtagatattc tacaacaaga gctaattgca gcaggggtgc
     7561 taccagaaga ttacgacttt gaatctcata ccgaactagt tccaatgcaa ccaggagatg
     7621 tgccagttac ttatgcagat actagtgcgt tagaacgaga ttttgaattt aaaccaagta
     7681 caagtttgcg tgacgggctt cgtaaatttg cgcagtggta taagtctttt tatgaagtat
     7741 aattgtattt ttttacttat aaaagtttat agaaacacct cagacactac aaatctgagg
     7801 tgttattttt taatctcgtt gaaataaatc tctggtatat actttttctt ttacatcatc
     7861 aagattacta tcgtaacgat tagcaataat ac
//
LOCUS       DACIRY010000118         7737 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.123, whole genome shotgun sequence.
ACCESSION   DACIRY010000118 DACIRY010000000
VERSION     DACIRY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.123"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            292..2523
                     /gene="pflB"
                     /locus_tag="IYE22_002238"
     CDS             292..2523
                     /gene="pflB"
                     /locus_tag="IYE22_002238"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ9507430.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2767..3528
                     /gene="pflA"
                     /locus_tag="IYE22_002239"
     CDS             2767..3528
                     /gene="pflA"
                     /locus_tag="IYE22_002239"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ9507431.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3613..4539
                     /locus_tag="IYE22_002240"
     CDS             3613..4539
                     /locus_tag="IYE22_002240"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ9507432.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4721..5614
                     /locus_tag="IYE22_002241"
     CDS             4721..5614
                     /locus_tag="IYE22_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ9507433.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5748..5873
                     /locus_tag="IYE22_002242"
     CDS             5748..5873
                     /locus_tag="IYE22_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507434.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(6136..7683)
                     /locus_tag="IYE22_002243"
     CDS             complement(6136..7683)
                     /locus_tag="IYE22_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ9507435.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT         2608 a         1370 c         1500 g         2259 t
ORIGIN      
        1 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
       61 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
      121 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
      181 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
      241 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
      301 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
      361 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
      421 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
      481 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
      541 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
      601 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
      661 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
      721 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
      781 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
      841 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
      901 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
      961 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
     1021 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
     1081 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
     1141 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
     1201 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
     1261 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
     1321 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
     1381 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
     1441 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
     1501 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
     1561 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
     1621 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
     1681 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
     1741 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
     1801 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
     1861 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
     1921 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
     1981 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
     2041 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
     2101 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
     2161 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
     2221 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
     2281 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
     2341 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
     2401 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
     2461 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
     2521 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
     2581 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
     2641 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
     2701 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
     2761 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
     2821 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
     2881 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
     2941 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
     3001 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
     3061 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
     3121 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
     3181 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
     3241 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
     3301 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
     3361 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
     3421 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
     3481 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
     3541 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
     3601 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
     3661 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
     3721 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
     3781 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
     3841 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
     3901 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
     3961 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
     4021 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
     4081 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
     4141 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
     4201 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
     4261 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
     4321 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
     4381 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
     4441 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
     4501 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
     4561 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
     4621 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
     4681 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
     4741 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
     4801 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
     4861 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
     4921 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
     4981 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
     5041 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
     5101 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
     5161 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
     5221 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
     5281 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
     5341 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
     5401 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
     5461 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
     5521 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
     5581 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
     5641 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
     5701 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
     5761 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
     5821 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
     5881 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
     5941 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
     6001 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatag
     6061 taagcgcccc atagaacagt acctgtacgt caaataaccc gacctataga atagaatcgg
     6121 gaatgtttgg cgtttttagt tacgagaaaa atgttgttgt acatccttgt tccaaggcaa
     6181 cagttgatct aaagtttctg ctgaaagtac gggaagattt ggtatcttat caaatagaaa
     6241 ctctagatac tttacaggat tcaatccgtt aagttctgct gttttatata cactcagaat
     6301 gattcctgaa gttcgtgctc cttcaagact tgtggaatgc aaccaatttt tccgtcctat
     6361 caccagctct ttgatatttc gctccgcacg attatttgtg agttctaaac gaccatcttc
     6421 aaaaataggt tcaaaaccac ttcgtagttt acacgcatat tgaacggctg catccaactt
     6481 ggattttgca acaggatcta tcgttgccat ccactcataa aaagcaataa ataatggacg
     6541 cagttcttgt tgccgacagc ttaatcgctt ctcaggagaa aagtctttcc atttcttttc
     6601 taggacaaac catttatcca attgtttgac tgcttgagct gcgggaatat ccgtgttttt
     6661 ctttcctttt ggaattgctt cgaaaaattt ccgccgaaca tgcgcgaaac aatagacaag
     6721 tcgtacactt tccacggcat tatacccgct atagccgtca cagtgtaaat accctgtaaa
     6781 ctccttgaga aaagttttcg gaacatctcc agcacgactt tcgtcgtggt gataataaac
     6841 gatgggatgc tgactgtctt ttcctgtaca aaactgccag taataggtat ctgttttggc
     6901 actttcaatc acacgataac ttgtttcatc cccgtgtaga atctcttctt gattaagata
     6961 gactcttaat cgttctccta aagggtctaa cgcgttttgc actgctttaa tatgccaatt
     7021 ggtgatatta tctctcgaaa tatccaaccc atgtgtttgt ttccaatagt tctcttgccg
     7081 ataggcagga actttctgac caaatttcat ccggatcgtt tccgccacta aactattgga
     7141 acctaagcta ttagaaatca aaggtttagg aacgatcgct tttttaaaag aagaaagtcc
     7201 ttctttttcg cagcttttac aacaataggt atgttgataa tgggtgagac aatccaacat
     7261 agcctgatgg aaacgaattt cttctcgtac ttttgtccgt ccgatatctt tcatctgtga
     7321 accgcaatgt tcacaaaatt gttcttccaa aggaaggaga tgttctttct ctgttatagg
     7381 taagtgtttg attttgtctg ctttcgttcc tacaactcgt tgatgtcctt ttactggaat
     7441 aatggtggca gctgcttctg gaacacttac agaaatttct tcatcactaa aaagattcaa
     7501 ttgcccattc gtttcctcaa agagagtctc tttagagcgt ccaaataatt tgttggttaa
     7561 ataattgacc ttttcattca atagttggat ttcagttgta agattctgaa tggtttgttg
     7621 ttgtgcttga ttggttttat gggcttcttg aagcaactga agcaacgtat ctgtagtttc
     7681 caaaattttt caccaccttg aatcgaactg attttgttac agaataccac aagaaac
//
LOCUS       DACIRY010000119         7700 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.124, whole genome shotgun sequence.
ACCESSION   DACIRY010000119 DACIRY010000000
VERSION     DACIRY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.124"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            544..1107
                     /locus_tag="IYE22_002244"
     CDS             544..1107
                     /locus_tag="IYE22_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9507436.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IYE22_002245"
     CDS             complement(1386..1673)
                     /locus_tag="IYE22_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ9507437.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IYE22_002246"
     CDS             complement(1663..1992)
                     /locus_tag="IYE22_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ9507438.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IYE22_002247"
     CDS             2297..2626
                     /locus_tag="IYE22_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9507439.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IYE22_002248"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IYE22_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ9507440.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IYE22_002249"
     CDS             complement(3551..4462)
                     /locus_tag="IYE22_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ9507441.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="IYE22_002250"
     CDS             complement(4890..5498)
                     /locus_tag="IYE22_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ9507442.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IYE22_002251"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IYE22_002251"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ9507443.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="IYE22_002252"
     CDS             complement(6528..7496)
                     /locus_tag="IYE22_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ9507444.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2351 a         1532 c         1281 g         2536 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ttaggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatta
//
LOCUS       DACIRY010000120         7640 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.125, whole genome shotgun sequence.
ACCESSION   DACIRY010000120 DACIRY010000000
VERSION     DACIRY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.125"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(123..647)
                     /locus_tag="IYE22_002253"
     CDS             complement(123..647)
                     /locus_tag="IYE22_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507445.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IYE22_002254"
     CDS             complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IYE22_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ9507446.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IYE22_002255"
     CDS             complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IYE22_002255"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ9507447.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2369..3049)
                     /locus_tag="IYE22_002256"
     CDS             complement(2369..3049)
                     /locus_tag="IYE22_002256"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ9507448.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3175..4044
                     /locus_tag="IYE22_002257"
     CDS             3175..4044
                     /locus_tag="IYE22_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9507449.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4070..4585
                     /locus_tag="IYE22_002258"
     CDS             4070..4585
                     /locus_tag="IYE22_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507450.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4632..5195)
                     /locus_tag="IYE22_002259"
     CDS             complement(4632..5195)
                     /locus_tag="IYE22_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507451.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5250..5429)
                     /locus_tag="IYE22_002260"
     CDS             complement(5250..5429)
                     /locus_tag="IYE22_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507452.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5506..6015)
                     /locus_tag="IYE22_002261"
     CDS             complement(5506..6015)
                     /locus_tag="IYE22_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507453.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6111..6491)
                     /locus_tag="IYE22_002262"
     CDS             complement(6111..6491)
                     /locus_tag="IYE22_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ9507454.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6821..7570
                     /locus_tag="IYE22_002263"
     CDS             6821..7570
                     /locus_tag="IYE22_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ9507455.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2173 a         1584 c         1358 g         2525 t
ORIGIN      
        1 agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc
       61 ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc
      121 ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag atctgttctt
      181 gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct
      241 ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc
      301 caactgatga tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga
      361 atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat
      421 gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg
      481 tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc
      541 aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca
      601 tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca
      661 tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac
      721 ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg
      781 cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata
      841 ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa
      901 cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg
      961 cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc
     1021 accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg
     1081 ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct
     1141 ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca
     1201 gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct
     1261 gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc
     1321 ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca
     1381 attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca
     1441 gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat
     1501 tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca
     1561 actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct
     1621 acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc
     1681 aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga
     1741 tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg
     1801 atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt
     1861 ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc
     1921 ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt
     1981 tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct
     2041 ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt
     2101 tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa
     2161 acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga
     2221 tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt
     2281 attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac
     2341 tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg
     2401 gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa
     2461 agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct
     2521 tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt
     2581 tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc
     2641 gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct
     2701 gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa
     2761 gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag
     2821 ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa
     2881 cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct
     2941 tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa
     3001 attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct
     3061 cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt caccctcagc
     3121 acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc
     3181 aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct
     3241 gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact
     3301 ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg
     3361 atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg
     3421 attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat
     3481 gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt
     3541 gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc
     3601 tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa
     3661 gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta
     3721 ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa
     3781 aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct
     3841 cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc
     3901 agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa
     3961 gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg
     4021 cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt
     4081 tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg
     4141 acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta
     4201 tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg
     4261 ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc
     4321 cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata tctataatca
     4381 tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt
     4441 ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg
     4501 tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt
     4561 ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca
     4621 ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc
     4681 tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca
     4741 tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct
     4801 aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa
     4861 taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga
     4921 tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa
     4981 ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg
     5041 taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct
     5101 gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt
     5161 tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct
     5221 aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta
     5281 atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact
     5341 ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt
     5401 ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc
     5461 ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca
     5521 tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt
     5581 gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt
     5641 catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc
     5701 ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac
     5761 ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt
     5821 ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc
     5881 tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc
     5941 ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa
     6001 catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga
     6061 acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc
     6121 agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta
     6181 tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca
     6241 atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt
     6301 tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat
     6361 ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta
     6421 acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt
     6481 gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag
     6541 ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa
     6601 ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat
     6661 gaaagtaaga aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt
     6721 tataattgaa agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata
     6781 cagaacacac tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt
     6841 tttagctcat cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtct
     6901 tggctgccag gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt
     6961 tatcggtacc acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa
     7021 cggtatttta ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa
     7081 ctatttaagt atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt
     7141 aagtaaagaa tggaatcata atatggcttc aaaaatccga aaattcactg gaaaaagctg
     7201 gtttatcgca atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg
     7261 ttatacagat gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc
     7321 tggtatcatc agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct
     7381 tgctcaaatg gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt
     7441 cgtgaacagc tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta
     7501 taaccaaaaa gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca
     7561 acacgcataa aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
     7621 aacacgtata aacggcggaa
//
LOCUS       DACIRY010000121         7600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.126, whole genome shotgun sequence.
ACCESSION   DACIRY010000121 DACIRY010000000
VERSION     DACIRY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.126"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(137..427)
                     /locus_tag="IYE22_002264"
     CDS             complement(137..427)
                     /locus_tag="IYE22_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507456.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IYE22_002265"
     CDS             complement(545..805)
                     /locus_tag="IYE22_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507457.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IYE22_002266"
     CDS             complement(942..1355)
                     /locus_tag="IYE22_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ9507458.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IYE22_002267"
     CDS             complement(1811..2296)
                     /locus_tag="IYE22_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507459.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="IYE22_002268"
     CDS             complement(2435..2650)
                     /locus_tag="IYE22_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ9507460.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="IYE22_002269"
     CDS             2874..3674
                     /locus_tag="IYE22_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9507461.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="IYE22_002270"
     CDS             3693..5561
                     /locus_tag="IYE22_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9507462.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="IYE22_002271"
     CDS             5554..6045
                     /locus_tag="IYE22_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ9507463.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="IYE22_002272"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="IYE22_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ9507464.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="IYE22_002273"
     CDS             6605..7600
                     /locus_tag="IYE22_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ9507465.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga
//
LOCUS       DACIRY010000122         7486 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.127, whole genome shotgun sequence.
ACCESSION   DACIRY010000122 DACIRY010000000
VERSION     DACIRY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.127"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            157..240
                     /locus_tag="IYE22_002274"
     CDS             157..240
                     /locus_tag="IYE22_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ9507466.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            365..1102
                     /gene="erm(B)"
                     /locus_tag="IYE22_002275"
     CDS             365..1102
                     /gene="erm(B)"
                     /locus_tag="IYE22_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ9507467.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1272..1532
                     /locus_tag="IYE22_002276"
                     /pseudo
     CDS             1272..1532
                     /locus_tag="IYE22_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1695..2360
                     /locus_tag="IYE22_002277"
                     /pseudo
     CDS             1695..2360
                     /locus_tag="IYE22_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2396..2641
                     /locus_tag="IYE22_002278"
     CDS             2396..2641
                     /locus_tag="IYE22_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ9507468.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2744..3613
                     /locus_tag="IYE22_002279"
     CDS             2744..3613
                     /locus_tag="IYE22_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ9507469.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3594..4328
                     /locus_tag="IYE22_002280"
     CDS             3594..4328
                     /locus_tag="IYE22_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9507470.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4361..5269
                     /locus_tag="IYE22_002281"
     CDS             4361..5269
                     /locus_tag="IYE22_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ9507471.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5266..5808
                     /gene="sat4"
                     /locus_tag="IYE22_002282"
     CDS             5266..5808
                     /gene="sat4"
                     /locus_tag="IYE22_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ9507472.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5901..6695
                     /locus_tag="IYE22_002283"
                     /note="aph(3')-IIIa"
     CDS             5901..6695
                     /locus_tag="IYE22_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ9507473.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6803..6991)
                     /locus_tag="IYE22_002284"
                     /pseudo
     CDS             complement(6803..6991)
                     /locus_tag="IYE22_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6971..>7486
                     /locus_tag="IYE22_002285"
     CDS             6971..>7486
                     /locus_tag="IYE22_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9507474.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         2617 a         1135 c         1563 g         2171 t
ORIGIN      
        1 aaatgattca tcgtcattcc tcctgctatc tttttctatt attctacctt atttgatagt
       61 agatttaaaa ctttgcaaca gaaccatttt gtgtataata ggaattgaag ttaaattaga
      121 tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca aatgcgtaat
      181 gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc agataaataa
      241 atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca gataactaaa
      301 attacaaaca aatcgtttaa cttctgtatt tatttacaga tgtaataact tcaggagtga
      361 ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa
      421 ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg
      481 taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt
      541 agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaacattc gtgtcacttt
      601 aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg
      661 gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccatgc
      721 gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg
      781 aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc
      841 ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac
      901 cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa
      961 tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa
     1021 agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct
     1081 attatttaac gggaggaaat aattctatga gtcgcttttt taaatttgga aagttacacg
     1141 ttactaaagg gaatggagat aaattattag atatactact gacagcttcc aagaagctaa
     1201 agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt cccactaagc
     1261 gctcgggacc ccttgtagga aaatgtccta agtgtggcaa caatattgta ttaaaaaaat
     1321 cgttttatgg ttgttcaaat tatcctgaat gtaagtttac tttagctgaa cattttagaa
     1381 agaaaaaact aaccaaaacg aatgtaaaag aattactgga gggaaaagaa accctggtaa
     1441 aaggaatcaa aaacaaagag aaaaagccct acaatgccgt tgtaaaaatt ggggaaaagg
     1501 gatatattga ttttatctct ttttcaaaat aaacatgggg ctgacccaaa agtagctcct
     1561 atatgaaaaa tagaggtctt tccatttaga tagtagtgga aagacctcta tttatctttt
     1621 gaaagtgaat acaaaaataa agcacactct gctataatat aggtgattaa tccatttgag
     1681 agaggtgctt tatcatgtat caaaattata ccataaacca actttgtttg cctatagatc
     1741 ttgaaattaa attagaagaa aatgattttg ctcatgccat tgtgcagttt gttgactcta
     1801 ttcccgatga agtctttctt ccttattatc aatctatggg tagaccccaa tatcatcctc
     1861 gtatgctact ttccattatt ctttgtgcct atattcaagg ggtttattcg ggccgccaaa
     1921 tacaaaatat gctgatagat agtattcgga tgcgctactt atctcaagag caattcccta
     1981 actttcgtac tattaatcgt tttcgcgttc atccgattat gaacgatatt ctagatcatt
     2041 catttgttca atttcgtgag ctactggttc aaagtggctt aatcagtggc agtgctctat
     2101 acattgatgg tacaaaaatt gaagcggatg cgaataaata ctctttcgtt tggaaaaaaa
     2161 gtattttaaa gtataaggat agccttgatc aaaaagccct tgaaaactac caacaaatgg
     2221 tggaaacaaa aatcttacct gagttaatag atgatttaaa ggatgaactt tctatagaag
     2281 agattgaaag gattcgtcaa tctttggaag aaaaggaaaa tcaattaatt gaagcgattg
     2341 accaaaccga aacagtagaa gggggctgac ccattattaa taaggagtgg acaacatgat
     2401 tgatgtaaat gagctattgg aagaagctat tagagaaact gaaaatctaa atgatggcga
     2461 agtttttctt gttaaagatt tattcaaggg atatgtgggg aataggatac ccagaaagga
     2521 tcgacttctg cttggaacgt tatttttaaa ccatgttagc aaaatgaatg gtaatttaaa
     2581 ggctattgaa aagacttcat ctaatcagca gaggtataaa aagacaattg ataagtttta
     2641 actatctttt gtgaaattaa aaaaattata aatatgttga gtagaagcaa aagatttggc
     2701 ttggaatttt tacagcagtg aatcgataaa aggagcatgc ttgatggtag ataatattat
     2761 taaatcagta gcagagaaat tatcctctct gtcttatata gaaggtattg ttttaggtgg
     2821 ttcacgtgca aggggcaccc atacagagga ttcggatata gatatcggca tctattacaa
     2881 ttcagaatca tttgacataa atactattaa tcaattcgct acaaagctgg atgatgagca
     2941 tagaaataac cttgttgtac ctcccggagc atggggtgat tggattaatg gcggcggatg
     3001 gttagtcata aacgggtatc atgtggattt aattttacgt gatattaaac gtgtggaaca
     3061 aataatgaaa gatacagagc acggaattgt tactgccaat tatcagactg ggcatcccca
     3121 tggttatatt agtgcaatgt atcgaggaga attagcgatt agcaaaatac tatatgctaa
     3181 gaatgaaagc ttatgcgaat taaaaaaaca ggcagaaact tatcccaatg ctttgcagaa
     3241 aagtttagtt aactttttta tgtttgaagc agggttctct ttaatgtttg taaaagcaaa
     3301 ttcgggaaca gacgataaat attatattgc gggtcatgtt tttcgtatag tttcatgttt
     3361 aaatcaagtg ttatttgcat gtaataatgc ttattgtatc aacgaaaaga aagctataaa
     3421 actgcttgaa acttttgaac ataaacctga aaaatatacc gagaaggtaa atcatatttt
     3481 tgaagtactc ggtatctcac tttttgaatg ctacgacatg accgagaagc tttataatga
     3541 agtgaatgaa attgtatcgg agataaataa ctttttaaac gaggagagtt cagatgaaag
     3601 aaaacaaata tgatgataat atattttttc aaaaatacag tcaaatgagt cgctcgcaga
     3661 aaggactggc tggtgcggga gaatgggaga ctttgaaaaa gatgctacct gattttaagg
     3721 gtaagcgtgt gcttgattta ggatgcggct atggatggca ctgtatatat gcgatggaaa
     3781 acggtgcttc ctctgtagta ggtgttgata tttctcataa aatgctcgaa gtagcaaaag
     3841 gaaaaaccca ttttccacag attgaatatg aatgctgtgc catagaagat gtggatttcc
     3901 cagaggagag ctttgatgta atactaagtt cgcttgcgtt tcattatgta gcagactatg
     3961 agaatttaat aaaaaagata tataggatgc tgaaggctgg tggcaattta gtttttacag
     4021 ttgaacatcc tgtttttact gctcatggaa cacaagactg gtattataac gaaaaaggag
     4081 aaatactgca tttcccggtg gacaattatt attatgaggg caaacggaca gctatgtttt
     4141 tggaagaaaa ggttacaaaa tatcatagaa cactgaccac atatctaaat acactgcttt
     4201 caaatagttt tataataaat cagattgtgg agccacagcc gccagagaac atgatggata
     4261 ttccggggat ggcggatgaa atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga
     4321 agatgtaata atatagaaaa aataaacgag gagtatgtaa atgagatcag aaaaagaaat
     4381 gatggattta gtactttctt tagcagaaca ggatgaacgt attcgaattg tgacccttga
     4441 ggggtcacgc gcaaatatta atatacctaa agatgaattt caggattatg atattacata
     4501 ttttgtaagt gatatagaac cgtttatatc taatgatgac tggcttaatc aatttgggaa
     4561 tataataatg atgcaaaagc cggaggatat ggaattattc ccacctgaag aaaagggatt
     4621 ttcctatctt atgctatttg atgattacaa taaaattgat cttaccttat tgcccttgga
     4681 agagttagat aattacctaa agggcgataa attaataaag gttctaattg ataaagattg
     4741 tagaattaaa agggacatag ttccgactga tatagattat catgtaagaa agccaagcgc
     4801 aagggagtat gatgattgct gcaatgaatt ttggaatgta acaccttatg ttattaaagg
     4861 attgtgccgt aaggaaattt tatttgctat tgatcatttt aatcagattg ttcgccatga
     4921 gctgctgaga atgatatcat ggaaggtcgg catcgaaaca ggctttaaat taagtgtagg
     4981 caagaactat aagtttattg aaaggtatat atccgaggat ttgtgggaga aacttttgtc
     5041 cacctaccgg atggattcct atgaaaacat atgggaagca ttatttctat gccatcaatt
     5101 gttcagggcg gtatccggtg aggtggcgga aaggcttcat tatgcctatc cggagtatga
     5161 taggaatata acaaaatata ccagggacat gtataaaaaa tacactggta aaaccggctg
     5221 cctggatagc acatatgccg ctgatataga agagaggcgg gaacagtgat tacagaaatg
     5281 aaagcagggc acctgaaaga tatcgataaa cccagcgaac catttgaggt gataggtaag
     5341 attataccga ggtatgaaaa cgagaattgg acctttacag aattactcta tgaagcgcca
     5401 tatttaaaaa gctaccaaga cgaagaggat gaagaggatg aggaggcaga ttgccttgaa
     5461 tatattgaca atactgataa gataatatat ctttactacc aagacgataa atgcgtcgga
     5521 aaagttaaac tgcgaaaaaa ttggaaccgg tacgcttata tagaagatat cgccgtatgt
     5581 aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat agaatgggca
     5641 aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct tatagcttgt
     5701 aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
     5761 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
     5821 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
     5881 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
     5941 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
     6001 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
     6061 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
     6121 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
     6181 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
     6241 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
     6301 atagcttaga cagccgatta gccgaattgg attacttact gaataacgat ctggccgatg
     6361 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
     6421 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
     6481 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
     6541 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
     6601 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
     6661 taaaatatta tattttactg gatgaattgt tttagtacct agatttagat gtctaaaaag
     6721 ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc aactgtccat
     6781 actctgatgt tttatatctt ttctaaaagt tcgctagata gagttctata ttagagatgt
     6841 aaagagtttt ggtgaagagg tttgtgagta tggcttttat aaccagggtg caatgagaag
     6901 cactaacagc agctagctta agaacgcata ggatatactt tttatggaag tcacagagga
     6961 ggaataaaat ttgaacagga ttaacagagt aacctctatt cttattcagc tgcaatcaaa
     7021 aaaaataatt cctgccaaag aaattgcaca gcgatttaat ataagcttaa ggacagttta
     7081 cagagatatc cggacacttg aagaagccgg aataccaatt ggatctgagg ccggaaaagg
     7141 atattttctt gtagagggct tcctacttcc gccggtaatg tttacagcgg cggaagtggg
     7201 tgcattgatt acagcaggga aatttttaaa ttgccatgga gatgaatcat ttataaagga
     7261 ttttgattca gctatgtata aaatcaaatc tattttgaag catggtgaaa aaaactatgc
     7321 acaggagttg gagaacagta ttaacgtgta cagcacatct ggacagaaaa atacattggc
     7381 ggataacgtt attgcagcaa ttcagactgc aatctgcaat aaaagggtaa tatcaattca
     7441 atatcctgca tcaggagggc aagaaccgga aagcagaatg atagag
//
LOCUS       DACIRY010000123         7407 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.129, whole genome shotgun sequence.
ACCESSION   DACIRY010000123 DACIRY010000000
VERSION     DACIRY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.129"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..740
                     /locus_tag="IYE22_002286"
     CDS             <1..740
                     /locus_tag="IYE22_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9507475.1"
                     /translation="FKFLFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRN
                     KRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYW
                     PFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLM
                     TILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGL
                     YQSVVGFVLLMITNTIARRFDSDSALF"
     gene            752..1672
                     /locus_tag="IYE22_002287"
     CDS             752..1672
                     /locus_tag="IYE22_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9507476.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1713..3170
                     /locus_tag="IYE22_002288"
     CDS             1713..3170
                     /locus_tag="IYE22_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9507477.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3515..4129
                     /locus_tag="IYE22_002289"
     CDS             3515..4129
                     /locus_tag="IYE22_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ9507478.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4122..5846
                     /locus_tag="IYE22_002290"
     CDS             4122..5846
                     /locus_tag="IYE22_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ9507479.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5843..7294
                     /locus_tag="IYE22_002291"
     CDS             5843..7294
                     /locus_tag="IYE22_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9507480.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2528 a         1232 c         1471 g         2176 t
ORIGIN      
        1 atttcaaatt tttattttct tcggatcaag catttctcat cactaaaaat acaattttat
       61 ataatgtagc atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga
      121 gcgagttgag aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt
      181 ttctatcttg ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa
      241 tattcaatca gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga
      301 aatattggcc gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca
      361 ttatttactt tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg
      421 atggagctag caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa
      481 tgacgatttt aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt
      541 ataacattcc taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata
      601 tctacaatgg cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc
      661 aatcagttgt cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata
      721 gcgattctgc attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca
      781 agtccgctct ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc
      841 tatttcctgt attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat
      901 attgtcgact cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta
      961 cttgttcgac cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac
     1021 tgttttaggt acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg
     1081 gtcaaacttt ccattccgtc gtttttttac cgttttttgt ttgattacga tgttgttctc
     1141 accagggatg gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat
     1201 ttgggcattg atcttgccaa tggctgtcag tcccttcaat attattgtga tgcgcacatt
     1261 ctttaaacgt tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga
     1321 actgcgaata tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag
     1381 tttatttgcc gcattgggtt actggaatga ctggttcaat gctttactat acatccaaga
     1441 tgataagcta gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct
     1501 gacacaaaat tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga
     1561 atctgccaga atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt
     1621 tttccagaag cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa
     1681 aaatataaat cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta
     1741 caggtgtttc agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag
     1801 accaggcaga agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag
     1861 ataatttcga tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa
     1921 aagtcaatat caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt
     1981 cttccgggga aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag
     2041 gagcctttgc tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt
     2101 tagatgaagc atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca
     2161 atggaaatgt ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg
     2221 atctttcaat tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc
     2281 atgaaaaaga accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga
     2341 actttgatta tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt
     2401 cgaaaatcat caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata
     2461 gctggtacaa acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac
     2521 tagaaggaaa tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata
     2581 caattttgac aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa
     2641 aatctacagc tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag
     2701 aaaaatcagt agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg
     2761 tttgggggat cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat
     2821 tgctagatgg ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga
     2881 ttctatatac acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg
     2941 ctgatgcaga aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag
     3001 aattaagtaa tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg
     3061 ttgatccaga tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata
     3121 aggtattgaa agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa
     3181 agacaaaggg gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc
     3241 aaaagccagt ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac
     3301 aaaatttttc ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt
     3361 tcaatcagct aaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc
     3421 cgtttgtttc tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg
     3481 agaaagtata aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt
     3541 atttatccgg atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc
     3601 cggcgggatc atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt
     3661 gtacggattc gagtataaag aaatcacaat aaaaagaggc tggaatatat ataaaagaaa
     3721 ttttttaaga ggcaatctgc tatttagttt atttttatct ggtactgctt tattgagtta
     3781 caatttatat ttatctgttc aaatcaaagg gctcattttt ttagctattg attttatttt
     3841 actttttgcc ttgctctgcc tatttgctac ttatcaatac agtatgattt tagatagtga
     3901 atatacgatc tctgttccta atctgctaaa gctaagtttt atttcggttt tttccagctt
     3961 ttcgtctttt ctaaaaataa ttattggtag cggtataatt ttaggcgtga cttggcaatt
     4021 caaaggattg atcctgtttg gtgtcattgg tcttttgacg gtttggaatg gtacgatgac
     4081 aacacattgg cgtgaagaat tagataaaca gttggaaagt tatgaataag tttcggattc
     4141 tattttcaaa aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg
     4201 gtgtaacgat gactgctacc gttattttta gtatccatac ttgggaacaa aatcaaaaac
     4261 aggcagaaaa catgacaagt gatgccgttc aaagcacaag cagaatgctg aatgataaaa
     4321 caaccttgag taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg
     4381 tgacgaccta cttaacaaaa cccatagatc agtatctgat gtatgtatac gaacagcaaa
     4441 acagtaccga tgaattagtt tctttcccaa atcaaatcaa agatctctac gcaaattatg
     4501 aagaactaag tgcgatatat atcgtgttga atcaactacc ggagtattat gaatcttcta
     4561 gagaaaacaa aggtggggaa ataaaaaagg ggacaccaaa attactagac gccttttata
     4621 tcaagttgcc tatcacacaa ggaggagctg agtcaggaag tatttttatt ggttttcaaa
     4681 agaaagaact agatatgatt ttagaaaatc taacgtcatt caatggactc tctttataca
     4741 tgatttctgg aacgacaaat cgactataca cattccatga taataaaata gctaaagaaa
     4801 cgtttgaaag acaagaaatg atcatcacag agagtctgaa gaaaaattca caattgccga
     4861 ttgacgatct ggaaaaaaac aattttgttc aacaccaaga actgtctgat gattatcgta
     4921 tattagctgt actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc
     4981 tgatcattgg tatcattatt ttagacaata tactgcttat ctttctttat aaaacattca
     5041 aacgatattc acagcaagta tctatcgtca tgcaggccat ggatcaaaca gcagcaggga
     5101 atttagagac gcggatcgac ataacaaaca cggaatatga actgaaggaa ctttcaatcg
     5161 gtatcaacga aatgttggat agcatcaatc agtttgtaga agacatctac aaattagaaa
     5221 tcaaacagca agacgcacat atgcgggcgc ttcaagcaca gatcaatcca cactttttat
     5281 ataatacatt agaatatatc cgaatgtatg caatcagcga aggaagtgaa gaattagcag
     5341 atgttgtgta tgcgttttcc gctttgcttc gaaacaatac gaatcaagag aaaacgatca
     5401 ctttaaaaga agaactggac ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc
     5461 ctaatcgagt agcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta
     5521 aatttgtgat ccagccatta gttgaaaatt attttaaaca cggtattgat tttactcgat
     5581 tcgataatgc gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg atcatcatca
     5641 aagataatgg aaaagggatg actgaaaaac ggctaaaaca gattgaagaa aaactgtctc
     5701 atcctaaagt ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg
     5761 ccagcttcgg cagcagttat tatatgtcgc ttgagaataa tgagacaggt ggactgaccg
     5821 tttctattac attcaaggag ggatgagcgt gtatcaagtg ttattggtag atgatgaata
     5881 catgattttg aatgggttaa aaaagataat tgattggcaa agtttaggct ttcaaatagt
     5941 agcaacagca gagaatgcca aagaaggact agctgtactt gaacagcgac agattgattt
     6001 agtagtaacg gatgtgacaa tgccagaaat aaatggactg gaatttattg aagcggctca
     6061 aaaagaacgt cataattttg aatttatgat tttatcagga taccaagaat tcgattattt
     6121 gaaaggaggt atgcaattag gcgccgtgaa ttatttgatg aaacccgtga ataagttcga
     6181 attggttgaa agcctcaaga aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa
     6241 tcaacaagaa atctatcaag aactgttatt cagtcaatgg ttgaatgaag aattggatga
     6301 aataagtgaa gaagaaataa taggacaaat aagtgagaag cagcatagga tagtattgat
     6361 tcaactggca agaactcatg atactcttat caatagatgg ttgcaagaac ggcaacaaat
     6421 cttttactat caacgaaatt acggagaccg tgtattgatc acgttgttgt tggaggcaga
     6481 tgaagttgat ggcttctgtt gttttgttca agaaaacttt cctgatcagg aatggctgat
     6541 cagtatcggg gaagagacag attctttgga caaaatccct gaaacattcc aacaagcgaa
     6601 agacaatcta cagttgcatc aattttatgg agataaagac gagcatgttt tttacgcagt
     6661 acaagcagaa acaaaagatc aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa
     6721 taaacaattg gaagtcgctc aaaaaatgat cactgatttt tttgaacagt ttcagttagc
     6781 agccatgatg ccggaagata tacgatattc cagtttttta ctgtttatgg agattcaacg
     6841 ggaattgatt gatctggaag atgaggaata tttacaagga atcgaaaaaa tccatcaggc
     6901 aaaaacggtg caagaacttc agcagctact tctgtcattc atcaaaaagc atcagagaca
     6961 gaaaaagtac agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga
     7021 accacttaca ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt atctaggaca
     7081 gctgttcaaa aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga
     7141 aaaagcgaaa caactgcttt tgcacagtaa tcaaaacatc aatgagatag caagtgagat
     7201 tggctacaac aacaccacgt atttctctaa attattcaag aaaatcgtcg gcagatcccc
     7261 taaagagtac cgagaaaaca gtggagaatc ataaaagaaa aagccaatgt aatgaaataa
     7321 cttataaaaa attgtctaga aatctaattt tgggctgttc ttattacttg aggcaaggca
     7381 gctttttcat gacctctcga taaacgg
//
LOCUS       DACIRY010000124         7331 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.130, whole genome shotgun sequence.
ACCESSION   DACIRY010000124 DACIRY010000000
VERSION     DACIRY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.130"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..412)
                     /locus_tag="IYE22_002292"
     CDS             complement(<1..412)
                     /locus_tag="IYE22_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ9507481.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGW"
     gene            497..673
                     /locus_tag="IYE22_002293"
                     /pseudo
     CDS             497..673
                     /locus_tag="IYE22_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(849..1418)
                     /locus_tag="IYE22_002294"
     CDS             complement(849..1418)
                     /locus_tag="IYE22_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ9507482.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1492..1815)
                     /locus_tag="IYE22_002295"
     CDS             complement(1492..1815)
                     /locus_tag="IYE22_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507483.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(1969..2781)
                     /locus_tag="IYE22_002296"
     CDS             complement(1969..2781)
                     /locus_tag="IYE22_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507484.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3135..3983
                     /locus_tag="IYE22_002297"
     CDS             3135..3983
                     /locus_tag="IYE22_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ9507485.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4128..5069
                     /locus_tag="IYE22_002298"
     CDS             4128..5069
                     /locus_tag="IYE22_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ9507486.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5059..7230
                     /locus_tag="IYE22_002299"
                     /pseudo
     CDS             5059..7230
                     /locus_tag="IYE22_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2191 a         1458 c         1320 g         2362 t
ORIGIN      
        1 gccaaccagc tacatgcaag tttgcgtttt cgatttctcc aaactgatca atgtgggcgt
       61 aatttgttcc atctgtcagc gtagaatttg cagcacctgc tcgagttgga tcaattactg
      121 gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa tcataggcta
      181 gtttttcttt acttacacct atttcagaaa gataaccgta aggatctgta tgatcgcccc
      241 aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa ggagtgtcta
      301 atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg tatactttat
      361 agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc acaggacttt
      421 tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa ggaaaacacc
      481 aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt tagaagatat
      541 caaaggtttt ttaggtcata aagatgtttc cactacacaa gtctatgccc atatttctcc
      601 cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc aaataaaaaa
      661 gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact aattttattt
      721 ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa
      781 ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca
      841 tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc catcaattga
      901 gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg cggttttctt
      961 cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc cacgactccg
     1021 ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc tagatttttt
     1081 gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag ctcttccatc
     1141 ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt tatttctttt
     1201 ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg caagagcgtt
     1261 tcgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa tatatatacg
     1321 ccagttcccg cgtctcgcgc ttgagcaaat acatccatcg gcgatcgttt cacttctgtg
     1381 atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc tttactaatc
     1441 gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt
     1501 tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg aaggatggta
     1561 cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcga tgatcactag
     1621 atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc gttttcgatg
     1681 tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt catagcgaaa
     1741 aaaaaagacg attttcactt caggagggtg gacatctaaa taccctcgtt cctggagtgt
     1801 tttcagtaaa agcatccccc actctactgc ggggtgtgca agtgcttgtt ggatcgctag
     1861 ccgaaattga tatagtttat ctttggaatt gggaaatttt tctaatgttt cctgataata
     1921 acgaacttct tctatcagcc ggtcatggat accaattacg taacgaagtc attcttggta
     1981 aagtactttt gtcagctttg atttgctttc gctctcaaat tcgctattca gccaaaacag
     2041 cgaatcaagc ttttcagtca caatcgtcaa tttccgatag tcttgtgtct cttctatccg
     2101 tttccaccgc ttttcagtaa tctggttaca gctcaagcgc aaaattgcta gataattttt
     2161 ttcttttatt ggcaaaatct gctcctctct aaatagcaat tcaggaaaca attcaaagaa
     2221 tagtgttcga ctgtctccta aaatctcgat ttctgccgga tcctttcttt gcttcaataa
     2281 ttggatgaac atctcagaaa aggactgctc tccatcatct gcaaaaaaat gcggaaaatc
     2341 tttcaaaaag caaaacagca gatatcttac ttttctttct gaaccaaaga tctgtgcttt
     2401 tttataagaa aaagaaaggt ggtactcgcc aaaccacttt ctcaatcgct cttgcgtaga
     2461 acgatcgtac ttctgctcgt aaggttcatt caatagcaga tttttcagta aacaaaacgg
     2521 gggtgattgg atcagaaaaa caaacaagag ataaggttcg ctatatcctt gatcataaac
     2581 taactgaacg gttttcttat gaagaatcag ctgaatggga cagtcgcttt ccgccaaacg
     2641 tttcagatgc cgatccactg ttttaacatc gattttcagt tcttcagcta aatcagcttt
     2701 tcgccagatc aaatctttca ttagattgat tagtgaaaat aaattctgtg cattttttga
     2761 taatagtttt tgccatctca tcgatcattt ctcacctgat ttctaaacat attgtttgat
     2821 caaaatgggt tcactcctaa aaagataaaa aactcccttt ctatttattt aaatacgcaa
     2881 atcttcctca ttatttttta ttttactcaa tttaatcgta ttctcatttc aaaacgaatg
     2941 tactttaaaa aaacataata agatcacttt gcttgtagga tgtattccct tccattacaa
     3001 tgaaataaat agcgatttta tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat
     3061 ccaaacaaaa aatcgatcag ttgatataag aattagaaaa agcggtcatc ggcaaacgtt
     3121 caactatcca attgatgatc gtggccctat tagcaggtgg tcatgtctta ttcgaagata
     3181 ttcccggtgt aggtaaaacg ttactggtca aagcactagc aaaagctgta cagggaacct
     3241 tcagcagagt ccaatgtact cctgatctgc tgcctagtga tattcttggt ttttccgtat
     3301 acaattcaca atcaaaagag tttgaatttc gtccgggacc gatctttacc acgatcttgt
     3361 tagcagatga gatcaatcgt acgactcctc gtactcagtc agcactttta gaagcaatgg
     3421 ccgagaatca tgcaacgatc gacaacaata cataccctct agacaatcat ttttttgtct
     3481 tagctacgca aaatccaatc gagtacgaag gaacatatcc tttgccggaa gcacagctcg
     3541 atcgtttttt attccgacta caaatcggtt atccttcttt tgatgacgaa cttttgctac
     3601 ttttggataa attcgaaaaa gtgttggata acttaaatga agtattgaac agttatgaaa
     3661 tagaagaaat aaaaacaaat gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg
     3721 ccttgcagtt agtgtcagca acaagaagtc atgctgccat ccagcttggc atcagtccac
     3781 gaggaagcct ttcctttatt caggcagcaa aagcttatgc tttaatccat gggagaaatt
     3841 acgtcacgcc caaagatttg caggatttag ttccttacgt gttttcccat cgcctgattt
     3901 tttacgatcg ttcattgaag gaagacgaaa agaaacaaat cattcatact cttgttgcac
     3961 aaattcctat tcctgtgagg tagattgagg tagacaatgg aaaaaatcaa agattatttc
     4021 attaattttg tgttatttct ggtatatttc atcattgtgc tttttgctct tgtattcaac
     4081 aattctttag gctggttctt gttggccttt tttactcttt atgtatcatg gagcttgatt
     4141 tcattaagca gctttctaaa gaaactttcg gttacttgta cattgcagcc actcgtttat
     4201 cgcaaaaaaa gtatgcaggt tattttttcc atagcaaaaa agagaacttt ttctttacct
     4261 ctccctcgtt tacaaatcgg cttcccagca atctttgcag aaaaaaacaa gagattttga
     4321 ttcttacaaa taaaccgcaa gaagtccaat tgctttggca gccaaaagaa agaggatgcc
     4381 atgaaacatt aactgtcatt tttacagcct atgatagtct aggacttttc agaaaaagtg
     4441 caaaaagaga gatctctttt cctgtgactg ttctgcctgc tttttgtaag attcaagcag
     4501 aaaaattaca gagagttctg gaaaaaaaac agcagctgaa cgcgtaccaa agaggtcttg
     4561 attttcggga acatcgcgct tatcgtccag gtgattcttt caaccgaatc gattggaaat
     4621 tgtctgctca tactcaagag tggttatacc gtgaatacga ttatcaagaa gaagaatgtt
     4681 ctcctctttt ttgcttttgg ggttcgccca gtccaaaatt tgaacctctg ctagacttgt
     4741 atttttcttt atggcatttg agaaaagaaa agcagccgga acttctgata ttaggcaacc
     4801 gcgcctttca tggcaaagac cccggtcata cctattttgc ttctttggaa gctggcgatg
     4861 agaaaacttt tttcgcacac ttgaaaaaat atgcaaaaaa acaaatcgtc ctctttgttc
     4921 ctgagaattc tcctgaagtc tcagaagcta ttgaaacatt gtcaaaagat cgaacggttt
     4981 atcttgttta tttcgtagaa aaacaactca tggtccaaga aaaagacaag atatgttcat
     5041 tgccaggagg tgaatggatt gacgattaaa cgggaaaaat ggctgcgtgg cgtttactcc
     5101 tttgtgatac taagtgtagg tgctccgata tttgtagtag ctaatcaatt gcaaaacagc
     5161 taccttctta tcctaactat tggtgtccta tgccttattg tctgctgtat tcctaaaaaa
     5221 tgggtacgtt tgctgctttc tttgccagta atgataggtt gtttgttttt atattttcct
     5281 tcgaagacat tttccatatt atggatctta cagttttttt cgtcagttac agaggaattc
     5341 ttccatctat cccaaggtaa actttattat ttgccagaaa agacagcttt ttttctcata
     5401 atgttgctga tctatttatt tattacattg accgtgcatt acgactattg gtacgcaccc
     5461 tttctttctt tgatggctta cttcatgatg cttaccgttt ttcggaaaaa agatttactg
     5521 tacgaaatga ttgcggtagt aactgttttg ttcgtgtatc tggcagcaag gcaatttttc
     5581 tccatccggt tgttgtcgag aaacactcga ttccagtcat tgactacagt tctgcttatt
     5641 tgtttcttaa cttttagcgc tatcttgcct ttatatcttc cgaaagtaca ccaatctctc
     5701 gaggaaacga gtgaaccgat ccgagaatat cttaatgatg aagggcttta tgacacactc
     5761 catgagtatc aattaaccgg aagtgcaaga acaggcttca gtgagaatga tgaacagctt
     5821 ggtggacctt tatatgataa tggcgaagtt gtttttacag cgaaccaaaa aggaaatggc
     5881 tattggaaga tcgaaaataa aaatcattac accggttctg gatgaataga agatcaggac
     5941 gcacccatcc aaccaatcca agaattccct gttgacatca cgagctacca aaccgttata
     6001 gaagacaaag aaaacattac attgacccta aaaaaacaag ctttcttacc ttatccttat
     6061 ggaaatacta ccttgactga tttttcatta gctagtggac aaacgcttta ctatttgcct
     6121 gaaagccagc gattcattcc tgtcccgaat gaatcgacac gttctgcaac tttacaaaca
     6181 gaaaggttag tttttacgag agaagaatta aaaagcagtc agttaaaagc tgatgattta
     6241 gaaaatcagc agacaaatct tcaattgcca aaaagtttgc ctaaacgtat tgaggacttg
     6301 gcggtcgaat tgaccaaaaa tgaagcaact ttaattcgta aagtcgaagc tgtctaacag
     6361 tatttaaaaa caagcggaaa tttccgctat tccaaaacgg ataccgggca tactccaaaa
     6421 gaacacgatt atgtggatta tttcctcttt gattcaaaag tcggctattg tgacaacttt
     6481 tcaacttcta tggtcgtcct tcttcgaaca cttggtatcc cagctcgctg gacaaaaggt
     6541 tttacaaatg ggctcagaac aggcacagat aatcaaggga atgaggaata cagcattctc
     6601 aatagtcatg ctcattcttg gccagaggta tactttcctg gatttggctg gcttccattt
     6661 gaaccaaccc cttcatttgc caatccaatg ggttcacaac cagttgctga aacaaccgaa
     6721 accgtccagc ctgctatcga acaaccaaat gaaagcgaaa atatggcaga aactatttcc
     6781 agtgagagat ctgaggtcat tccatcagaa gaaacaacac tcacaccagc ttataaaatc
     6841 gtcttattca gccttggagt gtgtttatcc gtcttgttgt tctttctatt gttattcaga
     6901 aaatctctgc gctggacaag ttatcttatc tatcttcatt tttttgcttc tactaatacg
     6961 attgcatatc aaaaacttct taaccctttg gaaacattgg taccgcgcaa acccgcagaa
     7021 tctttgcagg aatatagcct gcgaatagaa caagaattca agctagatct gcgtttcatt
     7081 cgattaacta ctcaatatga agcagaaata tatggacgac aagcagatcc ggtaaaattc
     7141 gataaaaaaa cctatttaga aataacgcat ataataagaa aaacggtata caaaaaaatt
     7201 ctgacaaaag ggagccgctt caaaaaataa gataaaattt ctggagaatc tacttctcga
     7261 atgccgagaa ccagctgatt gagtatcgat tactcgaaaa aaaggttgtg acacgttttt
     7321 tgtcacaacc t
//
LOCUS       DACIRY010000125         7330 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.131, whole genome shotgun sequence.
ACCESSION   DACIRY010000125 DACIRY010000000
VERSION     DACIRY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.131"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..400
                     /locus_tag="IYE22_002300"
     CDS             <1..400
                     /locus_tag="IYE22_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-aminoethylphosphonate:pyruvate
                     aminotransferase"
                     /protein_id="HAQ9507487.1"
                     /translation="ENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVL
                     RQKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHEC
                     FRIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            393..1178
                     /locus_tag="IYE22_002301"
     CDS             393..1178
                     /locus_tag="IYE22_002301"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ9507488.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(1524..3215)
                     /locus_tag="IYE22_002302"
     CDS             complement(1524..3215)
                     /locus_tag="IYE22_002302"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ9507489.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(3637..4584)
                     /gene="arcC"
                     /locus_tag="IYE22_002303"
     CDS             complement(3637..4584)
                     /gene="arcC"
                     /locus_tag="IYE22_002303"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ9507490.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(4699..5718)
                     /gene="argF"
                     /locus_tag="IYE22_002304"
     CDS             complement(4699..5718)
                     /gene="argF"
                     /locus_tag="IYE22_002304"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ9507491.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(5809..7038)
                     /gene="arcA"
                     /locus_tag="IYE22_002305"
     CDS             complement(5809..7038)
                     /gene="arcA"
                     /locus_tag="IYE22_002305"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ9507492.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2073 a         1507 c         1307 g         2443 t
ORIGIN      
        1 cgagaatggc aaatggcgtt tcacttcccc tacccatacg gttttggctt ttcaacaagc
       61 gctgaaggaa ctagaggcag aaggaggaat cgaaactcga tattcacgtt atctcgccaa
      121 taatcatgta ctacgacaaa aaatgacaga gatggggttc cagcctttca ttcaagaata
      181 tcaggggcca ttcatcacct cttttcttta ccctaaggac tggcgatttg attttaatca
      241 tttttatcag tatttaaagc aaaacggatt tgtgatctat cccgggaaaa tcagcgatca
      301 tgaatgtttt cgtatcggaa atattggcga gatctatccg gaagatattt tggaactgac
      361 agcaattatc aaagactata aggagaacgt taatgattaa aactgtaatt tttgattggg
      421 caggaacaac tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc
      481 tagaaaaagg aatccaactg actgacaaag aaatccgcga accgatggga aaattaaaat
      541 gggatcatat ccaacagctt ctttccctcc cttctgtcaa aaaacaatgg atagctattt
      601 atggacaact acctcaggaa aaagaggtgg aggaattgta tcaacaattt gaacattatc
      661 tgtttaaaga tttggtcaag cattctacct tgaaacccga cacattggaa acgatcgaac
      721 ggttgagaaa cgctgggatc aatattggtt ccacgactgg atttactgct cctatgatga
      781 caatcgtagc agaaaccgca aaaaaagaag ggtattcacc agacttcatg gcaacttctg
      841 aagacgtcga agggtatggt cgaccgtttc cttatatgat ccataaaaac atgcagtact
      901 tcaaaaacaa gtcggttgat gaagtaatca aagtcggaga tacggtctca gatatccagg
      961 aagggaaaaa tgccggagtg ttgacagtag gtgtaattga aggtagttcg ctaatgggga
     1021 tttcttatga tgagtaccaa aaattagaga tggggaaaaa agatcgttta aaacagtctg
     1081 ttgctcttag attccaacaa gctggcgctg atatttgtgt aaacaattta aaagagttaa
     1141 cagaatacat tttgtacgct gacaagaatc cagaatgact tttatacaaa atcagaggtc
     1201 gtgaaaaagc tgtactgcat caagtaataa gaacagccaa acaaaaatag ctcctcatat
     1261 ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
     1321 aaaaaaggga gctgtgacac gacttttgtc acagtcccct tatttacaaa caaacagtgt
     1381 ttaagagaca acctctccac taaacggtgt tcattcagct gactctcggt atttgctcaa
     1441 aaaacgataa aatttgaaaa gctattttct cttttgtttc tctaatactc ggatcagaac
     1501 gctgatttca cattctcttt ttcttacatc ttatctggtg catgaagtcc tagcaaacga
     1561 aggtcttctt tcaataatac agtcactgca taaactagag ctaagcgtgc ttctttttgt
     1621 tcatcatcag ctaaaatctt cgtatgagca tagtatttgt tgaatgcttg tgctaatttg
     1681 attgcatgtt tcgcaatcac tgacggttcg tatttttccc cagcagacaa gaccgtttca
     1741 gggtattttt gaactaattt aaccacttcc cagctggtat cgtcattcaa tgcatagtct
     1801 gctgtttctg aagggacaaa tcctgctttt tccaacaggc tgactgcacg agcatgtgta
     1861 tattgcacat aaggtccagt ttctccttca aatcgaacaa cttcttccag gttaaagtca
     1921 aatgtattca gtcgatcgtt tttcaaatcg tggaaaatga ctgccccaac cccaacttgt
     1981 ttagctacag catctttgtt ttccaaatca gggtttttct cgctgatttg ttctttagca
     2041 gattggattg cttcatttaa tacttcttct aataagacga tctttccttt acgagttgat
     2101 aattttttac caccttgcgt gatcaaacca aatggaatat ggcgcatatc gtcagaccag
     2161 tcaaagccca tttccttcaa gacagctttc aactgtttaa aatgatagct ttgttcattc
     2221 cctactacat atagagattg tttgaagtcg taagtacgtt tacggtacaa tgcagcagct
     2281 aagtcacgtg taatgtatag tgtcgcaccg tctgacttct taatcaacgc tggattcagg
     2341 tcataagcag ataggtcaac gatctctgca cctttatcct cgttcagcaa atgtttttct
     2401 tctaaaagtt tgacgatttc atccatcttg tcattgtaga aagcttcacc gttcaacgaa
     2461 tcgaaacgta cttccagcag atcatagatt ttattgaatt ctttcatcga ttcatcacgg
     2521 aaccattgcc ataattgaat agcctcttca tcgccttctt ccagacgttt gaaccaagca
     2581 cgagcttctt catttaattc tggttctgtt tcagctactt catggaattg gacatataaa
     2641 cgaagcaatt cattgattgg ttctgcttta acagcttctt ctgtacccca ttttttgtaa
     2701 gcgacgatca gttttccaaa ctgtgttccc cagtcaccta aatggttgat gcggattggc
     2761 tgataaccga ttttttccat aatgaaaccg atcgaattac cgataactgt cgaacgcaaa
     2821 tgtcccattg agattggttt ggcaatatta ggagaagaca tgtcgattgg tactgttcct
     2881 tgattcccga tattgctatc cccataatgt tctttttctt tcactactgt ttgcaaaact
     2941 tttttgctga taagttcttt attcatgaaa aagttcaagt aaggtccgac aacttcgatt
     3001 ttttcaaagt tggcagaatc gattttttct gctaaatctg cagcgatttg ctgtggcgct
     3061 tttcggtaaa cttttgctaa tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat
     3121 tttggatttt ctagtaattg ttcgacttgt tccagtgtta aatcatcttt gacagcgtca
     3181 tagacagctt ttgcgacaag atctttatta ttcatcttta ttcctcctaa agtgctccct
     3241 tgattgttaa acaaaaaaaa tctctagcga cggcatactt agtatgccat cactagagac
     3301 gagatttccc gcggtaccac tccaattgtc ctaaaaaagg accagctcca tctgatatcg
     3361 gtagacgacc gtctgtttaa aacagaattc tccaagtgcg cttcgttgta tcttcttcat
     3421 tcggcttcca ccacttccga attcgctagc caagaatgat acaagtactc tcttgttcaa
     3481 caaatcttag tcttattaaa ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt
     3541 cgtttctgat aaaaaaacaa tccagatggc ctaaaggaga agaaatacca cctggatagc
     3601 tataaatcat ttcgtaaata cgaaagactt aacaaattaa tcagctacga taatcgttcc
     3661 ttcttcagaa gctaaaagat tctcgatatt ttctaaagaa gtgatgattg ccttagcgtt
     3721 tggttttgct tcaacaaatg caatagctgc ctctactttc ggaagcatgc ttcctggagc
     3781 aaattgcttt tcatcaatgt attgtttcat ttctgaaaca gtaacagttt ctaatttctt
     3841 ttgatctggt ttttggtaat tgacatacac attgtctact cctgtcaata cgatcagcaa
     3901 atccgcatcg atgatttcgg ctaatttttc tgaagcaaaa tctttatcga ttacggcttc
     3961 tcgtccttct aatccagtag ctgtttctac aacaggaatc ccaccgccac caacagagac
     4021 tgtgataact ccttgatcga ccagcgtttc gatgacacgt gcttctttaa tcgagatggg
     4081 tttaggggaa gcgactactt ttctccatcc acgtccagca tcttctttga atgtcacagt
     4141 ggagtcagca ttcatttgtt ctttggcttc ttcttccgtg tagaatggac cgactggttt
     4201 acttggattt ttgaatgaag gatcattttc atccacaata acttgagtga ctaaagaaac
     4261 cacatcttta tcgatccctt tttccttcag tacttcgccc atggcatttt gtaaccagta
     4321 accgatagat ccttctgtca ttgcaacaca tgtatctaaa ggcattgccg gtgttttctc
     4381 agaatcagca gcttgctgct gaatcaatag gttaccgact tgcggaccat ttccgtggga
     4441 gatgatcaat tcatctcctt gttcgataaa tttcactaaa tactttgctg tttccattaa
     4501 cgcttcttgt tgggcttttg cacttgcatc cgtagacaag attgcattac cccctaaagc
     4561 gacaactact tttcgtttgg ccatttcatt cgtcctttct tcagttctac cgaattttct
     4621 agataacaaa acgagccagc gttttgtgat tttccctcaa tgctgactcg ttttttcgtg
     4681 agatgaatgt ggttatgctt aaacgcgagg gataaacaag ttacctaatg ttgctgccat
     4741 gatcgctttg attgagtgca tacgattttc tgcttggtca aattgacgag catatttgct
     4801 gcggaagact tcatctgtta cttccatttc agtgatgcca aagcgttctt tcatttgttc
     4861 accgtaaaca gtgtttgtat catggaaagc tggcaagcag tgcaagaaga tcaaacgatc
     4921 tgtgttgtgt gttttttcta ccatttccat attgatttgg taaggtttca acaatttgat
     4981 acgttcttcg aatttatctt cttcacccat tgatacccat acatcagaat aaaggacgtc
     5041 tgctccgtct acacctttat caacatcgtc ggtaatcatc aattgtgcac cggatttttc
     5101 agcaaagcct tctgccattt tgacgatttc ttcttcaggt tgtaattctt ttggtgcaac
     5161 gatacgcata ttcgctccta aaattgcacc tgtgactaat aaagagttag ccatattgtt
     5221 tcgaccatct ccgcagtaag caacagtgat accttctact gttccaaagt tttcttggat
     5281 agttaaaaag tcagcgatca tttgtgttgg atgccattca tcagtcaagc cattccaaac
     5341 tggaacacca gagaattctg ctagttcctc taccattttt tgactgaatc cacggaattc
     5401 gattccatca aacatacgac ctagtacttt agctgtatct tctgtggatt cttttttacc
     5461 taattggata tcatttgcac ctagatattc aggatgtgca cctagatcga tcgctgcagt
     5521 agtaaatgca gaacgagtac gtgtagatgt tttttcaaat aaaagcgcaa tattttttcc
     5581 ttctagatag tgatgtggga taccacgttt tttcaagtct ttcaagtgtt ctgaaaaatc
     5641 gatcaaatat tgtaattctt cttttgtaaa gtctttttct gctaataagc ttcttccttg
     5701 gaatactgat tctttcatat taaacattct cctttagatt gtttttattt aagattaaag
     5761 aggaaatccc gacggcagct gacagtaaga ccgccgtcag gaaacaaatt acttcaagtc
     5821 ttcacggaca agtggttggc tcatgcagcg tggaccacca cggcctcgtg acaattcact
     5881 tgaattgatt tctaacactt tgatgccata gctacgaagc aattcgttcg atacataatt
     5941 tcggttataa gtgactacaa cacctggagc gattgccaat gtgtttgatc catcattcca
     6001 ttgttctcga ggtgcaacga tctcatctcc attaccagtt gggatcagta caagatcatc
     6061 taatccaaga gcagctttca tcgtttcgtg taagttatct gaatgagtaa tcttgatatc
     6121 atcaccatct ggttcgattg taaagacatc catcttaccg tctttacttt gaattgctgg
     6181 atggatagtg aatttatcgt gatctaccat tgtaaatacg gtatccaaat gcatcattgc
     6241 acggttgttt ggaattttga ttgctaacac tttttcaaat cctgaatttt ttgcgaatag
     6301 gtggcgtgct aatttttcta aagctttcgc attagttcgt tgagaaatcc caacagctac
     6361 tactttatcg cttaagatca attcgtctcc gccttcgata tgatctggat tttcacgatc
     6421 caaccatact tcgattcctt tatttgcaaa tcttggatga tgtttcaaaa tatattctgt
     6481 aaacatcgat tcacgacgac gtgcttcaaa agtcatctta ttgactgtca tcccatttcc
     6541 cattgatgcg gaagggtcgc gtgtaaagta caagtttggc attgggtcca tgtaaaatgg
     6601 atagtcatcg tctgctgaca gatcgtatag actgctagaa cgtacttcaa tatcttttgt
     6661 acgaacccca gccatgattt tatctaccat ttcttgcgtt tccattgata gaaggaactc
     6721 atgaagaccc tctcttacag cattcgatgc aatatgagat tcatccaaca ttttattcaa
     6781 gaattgttct ttgacattgc ctgcgtcaat cgcttctgcc gctaattttt ccaagtaaag
     6841 agtctcgacg ccttcatttt gcaatgtctt tgcaaagttg tcatgttctt cttgagcaat
     6901 cggaagatat ggaatatcat cgaacaacag acgatccata atatctggtg tcaaattttc
     6961 cacttcttgt ccaggccttt tcagtaaaac tgtctttaat ttccctattt cagagaaaac
     7021 gtgaataggt ttatccatgt tgtgttgcct ccttcttttt gattacatgt ataggatatc
     7081 ctccttttgt aatcactttc ataattttgt ctcattcaaa atgagacatt cctcacatta
     7141 atattcgata attaaataat taaacgtttt tctattttta agcgtaattt attcattttt
     7201 tattctgtat acactttcaa aaaaatattt attcattgaa aaaaattttt tgaataaatt
     7261 aaaaaaataa attatattag cgttgttagc gtttactata tgtattattg ttgtttttta
     7321 tacacaaatc
//
LOCUS       DACIRY010000126         7306 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.132, whole genome shotgun sequence.
ACCESSION   DACIRY010000126 DACIRY010000000
VERSION     DACIRY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.132"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            138..3527
                     /locus_tag="IYE22_002306"
     CDS             138..3527
                     /locus_tag="IYE22_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ9507493.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3530..4951
                     /locus_tag="IYE22_002307"
     CDS             3530..4951
                     /locus_tag="IYE22_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ9507494.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4948..6825
                     /locus_tag="IYE22_002308"
     CDS             4948..6825
                     /locus_tag="IYE22_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ9507495.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6917..>7306
                     /locus_tag="IYE22_002309"
     CDS             6917..>7306
                     /locus_tag="IYE22_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9507496.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGAS"
BASE COUNT         2691 a         1378 c         1414 g         1823 t
ORIGIN      
        1 aaattggtgt gattcccttt agcgcccttc gtataataat tatcatatta aaataaataa
       61 aattatgtaa cgcctgttta ttttaataat tttcactatt cacgtcaaaa cagaaaaaaa
      121 gaaaaaggag gggaaaaatg acgaccacag ggaagaaact gaaagttatt ttcatgctga
      181 taatattgag tttatcaaac tttgtgccat tatctgcaat agcagacact acagatgatc
      241 caacagtttt agaaacaatt tcagctgaag tcatttcgga tcagtctgga aaaaaagcac
      301 tgaacatcaa gctaaatgcg aataacacca gtgctgaaaa gatagaaaaa gaaattggtc
      361 tagtcgaaaa ttacttaagt gatgtggaaa gaaaagaagg agatggctat gcttatcagg
      421 taaatagcgg gaaaattacg ttggaaatct catcaaacac taaacaaact atcgatctga
      481 gttttccaat cgatccagca ctttaccaca gccaggcaaa caagctgatc gtcgataata
      541 aagaatatga cattattgat gagacagaaa ataagaaaga tacagatgtg tcagtaccaa
      601 agccagacga aatagaagaa gaatcatcaa aagaaaacga aaattctgtc agcccattta
      661 cattgcctac attatccttg ccagctgtga gtgtgccatc taatcaaacg attcctacag
      721 aatatacaac agatgatcag ggcacttatc ctaaagccag ttggcaacct acaggaaata
      781 caaatgttct tgatcatcaa ggcaataaaa acggaacaaa tcaatgggat ggtataaatt
      841 cttggaatgg agatcctaat gatcggaccc attcgtatat cgaatatgga ggaaccggta
      901 atcaagcaga ctatgcgata cgaaagtatg caaaggaaac aagtacaccc ggattgtttg
      961 atgtttattt gaatgctcgt ggaaatgtac aaaaagatat cacgcctctt gatctcgtat
     1021 tggtcgtaga ctggtcagga agtatgaacg acaataatcg gatcggtgaa gtaaagattg
     1081 gtgtcgatcg ttttgtcgat actttagcag atagcggtat cacagacaaa atcaatatgg
     1141 gatatgtcgg ctactcaagc gaaggatata gctacagtaa cggtgcagta cagatgggtt
     1201 catttgattc agtgaaaaat caagtaaaat ccattacacc ttcacggaca aatggtggta
     1261 cttttacaca aaaagcacta agagatgcag gaagcatgct atccgttcca aatggacata
     1321 aaaaagtgat cgttttgctg acggatggtg taccaacatt ttcctataaa gtacagcggg
     1381 tacacgcaca atcaagcagc aattattacg gaactcagtt ttctaatacg caagatcggc
     1441 cgggaaatac ttctctaatc tcaagaatct atgatgcacc tgaccaaaac aatctatcca
     1501 gaagaatcga cagtacgttt atcgcaacca tcggagaagc gatggcactc aaagaacgag
     1561 gaatcgaaat acatggtctt ggcatccaac ttcaaagcga tccggcagct ggtctctcaa
     1621 aagcagaagt agagtctcgt atgcgacaaa tggtttcatc agatgaaaaa ggcgatcttt
     1681 actatgaatc agctgatcat gcaacagata tctctgaata cctagccaaa aaagctgtac
     1741 agatctcagc aactgtaagc aatggacaaa taaatgatcc aatcgcagaa ccattcattt
     1801 atcagcctgg tacactttca gtcaagagtg tggggacaag tcctacaacg gtcactccat
     1861 ctatttccat agaaggaaat accatcaaga gcaatcagat ctatttagga aaagaccaag
     1921 aaatccaaat ccattaccaa gtgagaatcc aaacagaaaa tgaggacttc catccaaatt
     1981 tctggtatca aatgaacggc aggacaactt tccagccaaa cattgatacc aatgaattag
     2041 ctgaattcgg tataccatct gctaaagctc ccggagtcag tcttcacatc aaaaagttat
     2101 gggaagaatt tgacaacaat ctagctgatc gtccagatca agttactttt gagattcaac
     2161 gggaacatac gacaaatgct gcagcttgga aaaacggata tattcgaatc attaaaccag
     2221 ctaaagatac aacaaatacg tgggaacgtg cagacattga caaattatct gcaaatagcg
     2281 gagaaagtta tcaagagata ttatcactac ctcaatacaa taatcaaggt caagcattca
     2341 gttaccaaac aatcaaagaa ttacctgtac caggatacga ttctcaacaa atagatgcaa
     2401 tgacatggaa aaatactaaa caattcacac cgttaaactt gaaaataacg aaaaattcct
     2461 ctacaggtga aaaggatctt attggcgctg ttttcaaatt aacaggagat tctattgata
     2521 ctttactaac agatcatggc gacggaacct attctcttcc agaaaatgtc aaattgcaaa
     2581 aagaaatgac ctatacgctg acagaaacaa aagctccaga agggcatgga ttaagcaaaa
     2641 agactacttg ggaaatcaag atcgcttctg atggtacggt aaccattgat ggaaaaacag
     2701 tcactacttc cgatgatacg atccagttga ctattgaaaa tccttttgtt gaagttcctg
     2761 tagcagtacg taagtatgcg atgcaaggga cggacaaaga gataaatctt aaaggagcag
     2821 cattttccct acagaaaaaa gaagcaaatg gtacttatca gccaattgac agccaaacaa
     2881 cgaatgaaaa aggtcttgcc agttttgatt cactcacacc tggtaaatat cgagtcgttg
     2941 aaacagctgg tcctgccgga tatgatactt cgccgggaaa ttatgaattc caaatcgata
     3001 aatatggaaa aatcatttac acgggaaaaa ataccgagat gacaaataat gtatggacgc
     3061 tcactcatca aaatcgacta aaagcgtttg atctaacggt acacaaaaaa gaagacaacg
     3121 gacagacatt aaaaggagca aaattcagac tgcagggacc agaaatggac ttagaatcgc
     3181 caaaagatgg acaagaaaca gatacctttc tattcgaaaa tttaaaacct ggaacttata
     3241 cgctgaccga aacttttaca ccagaaggat accaaggtct aaaagagcca gttactatag
     3301 ttatacacga agatgggtca attcaagtgg atggacaaga tcatgaatct gttctgtcac
     3361 caggagccaa aaacaaccag atttctttag acatcacgaa tcaggcaaaa gtaccattac
     3421 ctgaaacggg aggaattggc cgtttaggaa tctatctagt agggatgatt ggttgtgcgt
     3481 tttctatttg gtatcttttt ttgaaaaaag aaagaggggg cagctaaata tgaaaaaact
     3541 tggttggctt agtatgtgtc tcttcttgtt actatttaaa ccagctttta ctcaggtagc
     3601 aacagaaaca gaaacagaaa tggttcagat tactttacac aaattgcttt tcccaaacgg
     3661 gcaactgccg aaaaatcatc caaatgacgg acaagaaaaa gctttattac aaacgtatcg
     3721 aggattaaat ggtgtcacat tccaagttta tgatgtcaca gattcttttt accatctacg
     3781 ggaaaagggc aaaacggtag aagaagcaca agcagagatc gcaaaaaacg gtgcgtcttc
     3841 cggtatgttt accgcagaag caacaactac aactcttaac aacgaagatg gtatcgcttc
     3901 tttttctctg gccgctaaag atcaagaaaa aagagataaa gcgtatcttt tcattgaatc
     3961 caaagtacca gaagtcgtca aagaaaaggc agagaatatg gtagttgttc ttcctgtaca
     4021 tggacaaaac aatcaaaaac tttcaactat ccatttgtat cctaaaaatg aagaaaacga
     4081 ctaccctgat ccaccttttg agaaggtatt agaagagcct agaaatgatt ttacgattgg
     4141 tgaaaaaatc acttattcct tgcatacgac aattcctgta aatatccttg actatcaaaa
     4201 gttcgaattg tcagatagtg cggatgaagc attaacgttt ttacctaata gtttaacgat
     4261 ttcatcgaat ggagaaaagc tgacagaagg ctttgtcata cacaagaaac ctcacggatt
     4321 tgatgtttta ttttcgatcc cttcgttgga aaaatatgct ggaaaaaaac tgaccatttc
     4381 ttatcagatg cagctaagca gtacagcaca ggcgaacaag gaaatcaaca acaacggaac
     4441 actggatttt ggttttggtg tcagtacaaa gaaagtctct gtatatacag ggagtaagca
     4501 atttgtcaaa atcgagacaa ataaaccaga taaacgatta gctggcgcag tattccttat
     4561 taaaaacaaa gcaggaaatt acctccagca aacagccaac ggatacaagt ggacaaagaa
     4621 cgaatcagat gcgcttcacc tgatttccga taaaaatggc gctttttcaa tttccgggtt
     4681 gaaaacagga agttatcgat taaaagagat cgaagcacct tctggttata ttttaagtga
     4741 aacagaaatt ccgtttacca tttcaacttt tctttctgag gataaagagg cggacagtat
     4801 attgaaagta gtcaataaaa aagaaaatag ccgtccattt cttccaaaaa caaacgaaac
     4861 gaaaaataca cttttaggcg ttgttggtat ggtattcgca agctttgcaa tctggttgtt
     4921 tatcaaaaaa agaacaggag tgaaaaaatg aaaaatcata aaaaaataaa cgttatgtta
     4981 ggagtccttt tccttatttt accattactc acaaacagct tcggcgcaaa aaaagtgttt
     5041 gcagaggaga cagcagctca agtcatcctt cataaaaaga aaatgactga tttacccgat
     5101 cctttaatcc aaaacagcgg gaaagaaatg agcgaattcg atcaatacca aggattagcc
     5161 gatatttcat tttcagttta taacgtcact caagaatttt atgcgcaacg agataaagga
     5221 gcgtccgtgg atgcagcaaa acaagcagtc cagtctttga ctcctggtac accagttgct
     5281 tcaggaacga cagatgctga tggaaatgtc actttatctt tacctaaaaa acaaaatggg
     5341 aaagatgcag tctacacgat caaagaagaa ccaaaagacg gagtgtcagc tgccgcaaac
     5401 atggttttag ctttccctgt atatgagatg atcaaacaag cagatggctc ttataaatac
     5461 gggacagaag aactagatac tatccatctc taccctaaaa atacagtcgg taatgatgga
     5521 acgttgaaag ttacaaaaat cggtactgcc gaaaacgaag cactaaatgg agcagaattt
     5581 attatttcta aagaagaagg aacaccaagc gtcaaaaaat acatccaaag tgtcacagat
     5641 ggattgtaca cttggacaac tgatcaaacc aaagccaaac atttcattac tggtcattct
     5701 tatgacatcg gcaacaatga ctttgccgag gcatctattg aaaaaggcca gttgatcgtt
     5761 aatcatttag aagttggaaa atataattta gaagaagtaa aagctcctga taatgcggaa
     5821 atgattgaaa agcaaacaat cacgcctttt gagatcctgg caaatagcca aacaccagta
     5881 gaaaagacca tcaaaaatga tacgtctaaa gttgataaaa caacacctca attgaatgga
     5941 aaagatgtcg caatcggtga aaaaattcaa tatgagattt ctgtcaatat cccattaggt
     6001 atcgctgata aagaaggaac gcaaaacaag tacacaacat tcaaacttat cgatactcat
     6061 gacgctgctt taacatttga taatgattct tcaggaacgt atgcttatgc cttatatgat
     6121 ggaaataaag aaatcgaccc agtaaattat tctgtcactg agcaaacaga cggattcacg
     6181 gtttcagttg atccgaatta tattccttca ttaactcctg gcggtacatt gaaattcgtt
     6241 tactatatgc atttgaacga aaaagcagat ccaaccaaag gattttctaa ccaagcaaat
     6301 gtcgataacg ggcatacaaa tgatcaaaca ccaccgtcag tcgatgtcgt tactgggggc
     6361 aaacgatttg ttaaagtaga tggtgacgtt acatcagacc aaacacttgc tggagcagaa
     6421 ttcgtcgttc gtgatcaaga tagtgacaca gcgaaatatt tatcgatcga cccatccaca
     6481 aaagccgtca gctgggtatc ggcgaaagaa tcagcaacgg tttttacaac cacaagtaac
     6541 ggtttaatcg atgtgacagg tctaaaatat ggcacgtact atctggaaga aacgaaagcg
     6601 ccagaaaaat atgttccatt aacaaaccgt gtagcattta ctatcgatga acaatcttat
     6661 gtaacagcag gacagttgat ttctcctgaa aaaataccaa ataaacacaa aggtacactt
     6721 ccttcaacag gcggtaaggg aatctatgtg tatatcggtg caggagtagt ccttctactg
     6781 attgctggac tgtactttgc tagacgcaag cacagtcaga tttagtcttt ttgcaagaat
     6841 tggaaaaagg agcagcggat tacgcaaatg ctcctttttc ttttattatc aaacagtcat
     6901 cacaaggaga acacaaatga aaccaaagaa aaggaaactt ctagatctgc tgatgctttt
     6961 cttgcttctc tcaggaatag ggatactagc ttatccattt gttagcgacg cgctgaataa
     7021 ttatctggat cagcagatca tctctcatta tcagcaacaa gcggtcaaag aaaatgaaga
     7081 agtcatggct aaaatccaag aaaacatgac aaagaaaaac cagcaattag caaaagaagg
     7141 tggaaatcca ggtgcagatc cctttactaa gaaaaaagaa cctgcgaaaa aggatagaac
     7201 ctattttgag aaacatacga tcggtatttt gacgatccct gagatcaatg tccatttgcc
     7261 aatttttgat gaaacgaata ctgtgctgct agaaaaaggt gcctca
//
LOCUS       DACIRY010000127         7236 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.133, whole genome shotgun sequence.
ACCESSION   DACIRY010000127 DACIRY010000000
VERSION     DACIRY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.133"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            356..829
                     /locus_tag="IYE22_002310"
     CDS             356..829
                     /locus_tag="IYE22_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ9507497.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            826..2958
                     /gene="feoB"
                     /locus_tag="IYE22_002311"
     CDS             826..2958
                     /gene="feoB"
                     /locus_tag="IYE22_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ9507498.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2960..3094
                     /locus_tag="IYE22_002312"
     CDS             2960..3094
                     /locus_tag="IYE22_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ9507499.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3188..3832
                     /locus_tag="IYE22_002313"
     CDS             3188..3832
                     /locus_tag="IYE22_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9507500.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            4019..5212
                     /locus_tag="IYE22_002314"
     CDS             4019..5212
                     /locus_tag="IYE22_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ9507501.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5205..6932
                     /locus_tag="IYE22_002315"
     CDS             5205..6932
                     /locus_tag="IYE22_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9507502.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2360 a         1254 c         1599 g         2023 t
ORIGIN      
        1 tataaaactc ttagtaaaaa agtcaaacaa caaacactac atattgtgtt gtggaaactg
       61 tggaatacta agttttgtgt ttttgaaaaa agagctgaaa acaagtatcg tgtgggaaaa
      121 tagcaaattt aatagagaaa aaattgtgga aaactatgaa gaaatatttt tttctcattt
      181 ttatctatca atactaaaga ttcgctcata atatcacaag tataattgac attctcaatt
      241 accgagagta ttctatgtga gaatgattct catttatttg cgtaacacgt aagaaaatta
      301 gaacatataa tgaatgattt taatacatga tttatttcac aagagaggaa tcaatatgca
      361 gaccttagcg caggcaaagc agaatttcgt ttatcaagtc gaacgaatcg aaacagaaga
      421 aaaaacaaaa cgccatttgc aaaatatcgg gatcgttgcc ggaagtaaaa tcgtcgtcgt
      481 caatttatca ggcaacaatg ccattttact ggtaaaaagt catcgagtgg cagtaagtcg
      541 ggaaatacta ggtcaaatct ttgtcaataa aaagactgct tcctctgaaa gctggacttc
      601 tcttgatcaa ttaaaagcag gagagactgg gatcgtcgta gcaatccacg gacaaggcgc
      661 agtgaagcga cggttaatgg atatgggatt gacgcgaaat gtaaaaatag ttatccgaaa
      721 actagcccct ctgggagatc caatcgaact gacagtgcga gggtatgaac tgacgttacg
      781 aaagtcagag gccgaactaa tcttgataca aagggaggaa aaagaatgaa tatcgcgtta
      841 gtcggaaatc caaacagcgg gaaaactagc ttgttcaatc tcttgactgg ttcgaatcaa
      901 tatgtaggga attggccggg cgtaacagta gaaaaaaaag aaggatcgtt gaaaaaaaat
      961 ccgaacattc atattcagga cctacccggc atctattctt tgtctcctta tacaccagaa
     1021 gaagtgattg caagagatta tttggttcag gaacagcctg aactgatcat caacattatt
     1081 gatgctacca atttagaacg aaatctttat ttaactacgc agttattgga tttagacatt
     1141 cctatgatca ttggtctgaa tatgatggat gtcatcaaaa aagaaggaaa aaaaatcaat
     1201 ctagaaaagc tttcctatgg tcttggtgta ccagttttac caatcagcgt attgaaaaag
     1261 caaggaatcg aaaaactaat cgaaaagatt caggagtctg caaaaaagcg gccttctatt
     1321 cctgttccct gtacatatga tccacgttta gaagcagctt tacatgaaat tgaagatgta
     1381 ctaggagaac aaagcggcaa gaaacgctgg tatgcaatga aatattttga acgagatcag
     1441 aaggtaagag aggattacga gatagcagtt tctcagcaaa aagagatcga gcaactgatc
     1501 cagttaacag agaaactatt ggacgacgat agtgagacga ttctggtcaa tgaacgttat
     1561 gaatttatta cccaactatg tacactctgt gttgtgtcaa atgattcttt ccaattatcg
     1621 atgagtgaca aaatcgatca gattgcaacg aatcgttggt tagccttgcc gatcttcgca
     1681 tttgtgatgt ggctgatcta ttatctggct attcaaacag taggaacaat ggggacagat
     1741 tggatcaatg acacattgtt tggtacatgg ttaccagaac atgttggcag attgttagct
     1801 gaatggcaag tagcgggatg gatgcaggag ctgatcttga acggcatcat cgcaggcgtg
     1861 ggagcagtgt taggatttct accgcaattg attatattgt ttttatgctt gagctttctg
     1921 gaagactgcg gatatatggc gaggatcgct tttgtcatgg atcgactttt caggaaattc
     1981 ggtctttcag gaaaatcctt cattcctatg ctgattgcta caggatgtgg agtgccaggc
     2041 gtcatggcca gtcgcacgat tgaaaacgag caagatcgtc gtttgacgat catggtcaca
     2101 accttcatgc cttgttctgc aaagttgccg attattgcct tagtagcagg ggcctttttt
     2161 cctcatcaat cttgggtagc tccttctgcc tattttcttg gtatgactgc aattattttc
     2221 tctggaatca gcttgaaaaa aacacgtttg tttgctggag atacagctcc ttttatcatg
     2281 gaattgcccg catatcattt tcctcaatgg tcagcggtcc taagacaaac ctatgaccgc
     2341 agcaagtctt ttgttaaaaa agcaggaacg attatctttg tttctagtat cgtgatctgg
     2401 ttcagctctt cttataattt ttatgctcaa cctgtttctg aagaccaaag tatcttagcc
     2461 tttttcgggc gaatcctggc agttgttttt cagccgcttg gttggggaaa ctggcaagga
     2521 acagtcgcag cgattacagg attggtcgct aaagaaaata ttattggcac atttggtatt
     2581 ttgtttggtc atgcggaagt atccgaaaat ggcagagaaa tctggcaagt gctacaacac
     2641 acatatactc ccgcagcagc ttattcattg ttagccttca atttactttg tgcgccttgt
     2701 tttgcggcaa ttggggctat ccatcgggaa atgaatgcca agaaatggac ttggatcgcc
     2761 ataggctatc aatgcggttt agcttattta gttagttttg tgatttatca gttgggacac
     2821 ttattggagg gtggacaggt agcgttaggg acgtatcttg ctattttact gatgatagga
     2881 ctcgtttatg ttttgttccg tcagccaaaa tccaagaacc agttttatac tatattgtca
     2941 ttagaaggag aggaatagga tggcgacatt cttattaagt gtgttaatct ttggaagtgc
     3001 aggcgcaatt gtctatactc gtttaaaatc cggtaagaat tgtgatgact gtcaaacagc
     3061 ttgcccagta aaaaaggaac agatcaagcg atgatcagtc gttgcaagag gctattctcc
     3121 ccttttttgc ttattttgct agagcatagt aagatgaggg aaagtaaaaa aagaaagagg
     3181 aaagaaaatg tctggaaaga aaaaaatcgt ccgcccgcgc taccagcaga ttgctgttga
     3241 tttagccgaa cgtatcgttg aaaaccgata taaagtgggt gagaagatcc atgcccgctc
     3301 aacacttgcc agtacgtaca atgcttctcc tgagactacc cgcaaggcta tcaatgtgct
     3361 ggtagatctt ggaatcatgg aagtacggca tggaagtggg gcatttgttg cttcgaagga
     3421 aaaagcaaaa gatttcctat tgcaatatca agatgtacaa tccatccaag atacaaaagc
     3481 agaaatcatg gcaagtgtgg agaagcaaca agatgagctg aatcattttt ctcaattatt
     3541 ggatcagctg gtcaatcaaa cgaagcgaat acataagatg aatcctttac ttccgtttga
     3601 attatcttta tcagaaaaag caatgcatct ggaccgttca attagcgagt tgaatatctg
     3661 gcaggcgact ggcgccacag ttatcgcaat ttctcataat gaagaactgc ttgtttcacc
     3721 tggcccctat gctaaattta cggcagggga catcgttcat tttattggaa atgaatatac
     3781 actacagcgt atgacaaact ttttttaccc ggagcatcag cttgcagaat gattgtgtga
     3841 aatattaaaa aaatattaac ttggctaaac cttttattta caaagattta gccaagtttt
     3901 tttcagtttg gacaagcaaa tttctttcat ttttgacaaa gtgactacat gatgttaaca
     3961 tagtgtggtc acttttttca gaaagaaaaa tgaatttaca taaaaaagga gaatttgttt
     4021 ggtaaaagta gaagtaaaac atttaacaaa gatatttggt aaaaagacac aagccgcttt
     4081 agatatgatg aatgatcacc agccgaaaac ggagatttta aagaaaaccg gtgcaacggt
     4141 tggtgtgtat gacgtgaatt ttgacgtaaa agaaggagaa atcttcgtga tcatggggct
     4201 ttccgggagt gggaaatcca cattgatccg tctattgaat cgtctgattg agccaacttc
     4261 gggcagtatc tatattgatg gagaagatgt atcgaagtta agcaaagaag aattgcgaga
     4321 agtccgtcgt cataaaatca atatggtttt ccaaaacttc ggtctgtttc cacatcgaac
     4381 gatcttggaa aatacagaat acgggttaga agtccgtggt gtacctaaag aagaacgaca
     4441 agaaaaagca gaaaaagcat tagaaaattc tagtttgctt tcatttaaag accaatatcc
     4501 tagtcagtta tctggtggga tgcaacaacg ggttggccta gccagagctt tagcaaatga
     4561 tcctgagatc ttgctgatgg acgaagcgtt ctcggctctt gatccgctga tccggcgaga
     4621 aatgcaagac gaattactgg acctgcaagc caatgttcaa aaaacgatta ttttcattac
     4681 ccatgatttg aatgaagctt tacggattgg ggacagaatc gccttgatga aagacggcga
     4741 aatcatgcag atcggaacag gtgaagaaat attgaccaat ccagcaaatg attatgtacg
     4801 tgaatttgtg gaagaagtcg atcgttccaa agtgcttacg gctcaaaaca tcatggtacc
     4861 tgcattgacg acgaatatcg aatctgatgg accaaacgtt gcattgacaa ggatgcgtaa
     4921 tgaagaagtc agcatgctga tggctgttga cagaaagaga cacctgaaag ggatcatcac
     4981 tgcagatcaa gcgttagaag ctaggaagca aaaacgtcca ttgatcgact tcctagatga
     5041 aaatgtgacg gtgattggga aagacatggt ggtaagtgat attttcaata ttatttatga
     5101 ctcaccgact ccacttgcag ttgtagagga tggaaaatta aaaggtgtcg tcatccgagg
     5161 tagtgtgatt gaagcattag cagaaacaag cgaggtgagt gaacatgaat aatctattga
     5221 actatcaatt accagtggca aactgggtag aaaatatcac agaatggttt acgacaactt
     5281 tttctggcct attttcattt ttacaaacga ttggacaggc agtgatgtcg gggattacaa
     5341 atttactgtt agtgattcca gctcctttgt ttatcttgtt attgacggta gcagctttct
     5401 ttatttctaa gaaacgtcca ggacttacgt tgttcacatt aatcggtcta tggtttattt
     5461 ataaccaagg tctatggaat gatttgatga atactgtcac acttgttctt ttatctagtg
     5521 tgatctcgat catcattggg gtaccattag ggatcttgat ggcaaaaagc agtaaagccc
     5581 aaagtattat caaaccgatc cttgacttta tgcagacaat gccaggattc gtttatttga
     5641 ttccagcagt tgcatttttc ggtatcggga tggttccagg ggtatttgcc tcagtcatct
     5701 ttgctctgcc accaaccgtt cgttttacga atttagggat tcgacaagtt cctaaagaat
     5761 tagtagaagc ttctgattca ttcgggagca caagtcgtca aaaactattc aaactagaat
     5821 tgcctttagc taaaagtacg atcatggcag ggatcaatca aacaacaatg ctgtcacttt
     5881 caatggtcgt tattgcctct atgatcggtg cacccggctt aggacgcggc gtattatcag
     5941 cattgcaaag agcacaagtc gggaatggat ttgtcaacgg ggttgcatta gttatcttag
     6001 caatcatcat tgaccgtttt acacagcatt tgaaccagcc aaacaataaa aaagctgcag
     6061 gagcaacaac acagaaaagt aaaaaacgtc aaggactgat cattggtgct gtagtagtag
     6121 tgatcttagg tgcgattggt atagggagtt tttcatctgc caaagaaaca aaacgtatca
     6181 acttatctta tgttgaatgg gatacagaag ttgcttcaac aaatgtagtc ggtgaagtat
     6241 tgaaacaaat gggctatgat gtaacgatga cgccattaga taattcaatc atgtggaaat
     6301 ctgtttcaaa tggcgagtca gatgccatgg tttcagcttg gctacctaaa actcatggtt
     6361 cacaatatgc acaatataaa gatcaagtcg aagacctagg tgccaatttg acaggagcaa
     6421 aagttggttt agcagtgcca gcctacatgg atgtaaattc gatcgatgag ttaaccgatc
     6481 aggcaggtaa gaaaatcata ggaatcgagc caggagctgg tgtagttacg gctgctgaaa
     6541 acacgatcca aaaatatgac aacttgaaag actggaaagt agagacatct tcttcaggag
     6601 caatgaccgt tgcactaggt caagcgatca aaaaacacga accaattgta gttacaggat
     6661 ggacacctca ttggatgttt gccaaatatg atctgaaata cttagaagat cctgaaaacg
     6721 gaatgggaag cgaagagcag atccatacga tggttcgaaa aggattgaaa gaagaccagc
     6781 cagaagctta caaagtatta gacaatttcc attggtcaga aaaagatatg gaaaaagtta
     6841 tgttggaaat caacaatgga aaagatccac agcaagcagc aaaagactgg atcaaagaga
     6901 accaagaatt agtcgaaagc tggaaaaaat aaaaagacag acacgaaata aatggagctt
     6961 ccaaaattgg atcaaatatc caaatttgga agctccatgt tcgtctctat cttttttgtc
     7021 tataaaaaaa gagaatgcgt agctcatctt cgaaacgttc aaagacgccg acaaactatt
     7081 atgaatttat gggtgcgagt caaagagatc gatcgtaggt atgaaagact aatgtgatta
     7141 aagtttatta tgtacgaagt gcattctcaa aaaatattaa agaatataca tagtttatat
     7201 acttaaaagt gattaaatga taaagtccgt ataatt
//
LOCUS       DACIRY010000128         7158 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.134, whole genome shotgun sequence.
ACCESSION   DACIRY010000128 DACIRY010000000
VERSION     DACIRY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.134"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(560..1600)
                     /locus_tag="IYE22_002316"
     CDS             complement(560..1600)
                     /locus_tag="IYE22_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ9507503.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IYE22_002317"
     CDS             complement(1949..2275)
                     /locus_tag="IYE22_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507504.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IYE22_002318"
     CDS             complement(2262..3050)
                     /locus_tag="IYE22_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ9507505.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="IYE22_002319"
     CDS             complement(3418..3708)
                     /locus_tag="IYE22_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ9507506.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="IYE22_002320"
     CDS             complement(3844..4023)
                     /locus_tag="IYE22_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507507.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="IYE22_002321"
     CDS             complement(4020..4370)
                     /locus_tag="IYE22_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507508.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="IYE22_002322"
     CDS             complement(4363..5688)
                     /locus_tag="IYE22_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ9507509.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="IYE22_002323"
     CDS             6138..6752
                     /locus_tag="IYE22_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9507510.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..>7158)
                     /locus_tag="IYE22_002324"
     CDS             complement(6917..>7158)
                     /locus_tag="IYE22_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507511.1"
                     /translation="SFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGIS
                     ITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         2188 a         1292 c         1062 g         2616 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctat
//
LOCUS       DACIRY010000129         7151 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.135, whole genome shotgun sequence.
ACCESSION   DACIRY010000129 DACIRY010000000
VERSION     DACIRY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.135"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(98..1228)
                     /locus_tag="IYE22_002325"
     CDS             complement(98..1228)
                     /locus_tag="IYE22_002325"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ9507512.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IYE22_002326"
     CDS             complement(1877..3079)
                     /locus_tag="IYE22_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9507513.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IYE22_002327"
     CDS             complement(3139..3342)
                     /locus_tag="IYE22_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ9507514.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IYE22_002328"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IYE22_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IYE22_002329"
     CDS             4879..5691
                     /locus_tag="IYE22_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9507515.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IYE22_002330"
     CDS             complement(6715..6867)
                     /locus_tag="IYE22_002330"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507516.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            6930..7148
                     /locus_tag="IYE22_002331"
     CDS             6930..7148
                     /locus_tag="IYE22_002331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507517.1"
                     /translation="MKTTRFDLEPFYLNSFSLYPTSFDHLITRLNTFDMSINETFINY
                     GCLFLTAFLANANPPIIPALSPNSGVIM"
BASE COUNT         2201 a         1430 c         1102 g         2418 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct a
//
LOCUS       DACIRY010000130         7054 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.136, whole genome shotgun sequence.
ACCESSION   DACIRY010000130 DACIRY010000000
VERSION     DACIRY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.136"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..3035
                     /locus_tag="IYE22_002332"
     CDS             <1..3035
                     /locus_tag="IYE22_002332"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ9507518.1"
                     /translation="TIIELAAVKMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMV
                     RGSKSEEEVLRMFLEFSKDTILVAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELAR
                     YLYPQFKRFGLGVLSKKFGVSLEQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQL
                     NEHIGEGDSYKRARPFHVTILAKNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMR
                     ENLLIGSACDKGEIFEAMMQKGVEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHD
                     LEEIIQNLVEIGKSLDKIVVATGNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVH
                     FRTTDEMLTAFHFLGEETAKEIVVENTNKIADICEEVIPVKDELYTPKIPGSEDEISE
                     LSYTKAKQMYGDPLPEIIQKRLEKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSR
                     GSVGSSFVATMTGITEVNPLAPHYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARL
                     NKDGHDIPFETFLGFHGDKVPDIDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVA
                     DKTAYGYVKGYERDNNLQFRSAEVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFT
                     PIQYPADDQNSEWKTTHFDFHSIHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTD
                     DPEVMRIFAGPEVLGVSQEQIYSKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISG
                     LSHGTDVWLGNAEELIRRGDATLAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQ
                     WNKIPDELRETYLSAMKENNVPDWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYF
                     PILYYCAYFSVRADDFDLVSMCKGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANE
                     MLERGFKFGMIDLYKSDAVNFVIEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSK
                     EDLATRGKVSKTLIEYMNDNGVLKDLPDENQLSLFDML"
     gene            3179..3652
                     /gene="rimP"
                     /locus_tag="IYE22_002333"
     CDS             3179..3652
                     /gene="rimP"
                     /locus_tag="IYE22_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ9507519.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            3676..4851
                     /gene="nusA"
                     /locus_tag="IYE22_002334"
     CDS             3676..4851
                     /gene="nusA"
                     /locus_tag="IYE22_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ9507520.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            4873..5166
                     /locus_tag="IYE22_002335"
     CDS             4873..5166
                     /locus_tag="IYE22_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ9507521.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            5163..5474
                     /locus_tag="IYE22_002336"
     CDS             5163..5474
                     /locus_tag="IYE22_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ9507522.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            5487..>7054
                     /gene="infB"
                     /locus_tag="IYE22_002337"
     CDS             5487..>7054
                     /gene="infB"
                     /locus_tag="IYE22_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ9507523.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVV"
BASE COUNT         2467 a         1287 c         1554 g         1746 t
ORIGIN      
        1 acacgatcat cgagctagcc gcagtcaaaa tgtataaagg aaatgtgatc gaaagctttg
       61 atgaatttat cgatccagga catccattgt cacgaacaac ggtagatctg actggtatca
      121 cggatgaaat ggttcgaggg tcaaagtcgg aagaagaagt attgcggatg ttccttgaat
      181 tttcaaaaga tacaattttg gtagcccaca acgcggcttt tgatatgggg ttcttgaata
      241 ctagctacgc aaggtatggt attcctgaag cggccaatcc agtcattgat acgttggaat
      301 tagctagata tctgtatcct caattcaaac gtttcggatt aggtgtgtta tctaaaaagt
      361 ttggtgttag cttggaacag catcaccgag caatctatga tgctgaagca actggtcact
      421 tagcatggat ctttgtgaaa gaggcaatgg acaatcataa catgctttat catgatcagc
      481 taaatgagca tatcggagaa ggagattctt acaaaagggc tcgacctttc catgtaacga
      541 ttttagcaaa aaatcaggca ggactgaaag atttgttcaa actgatttca atgtcgaatg
      601 tcgaatactt cgaacgcgtc ccacgaatac cacgctccca attgaaaaag atgcgtgaaa
      661 atctattgat cggctcagct tgtgataagg gagaaatatt cgaagccatg atgcaaaaag
      721 gggtagaaga ggcaagaaac cgagcaaaat tctatgatta tattgaagtt atgccaaaag
      781 cggtctatgc gcctttgatc gagcaagaat tggtaaaaaa tgaacatgat ttggaagaaa
      841 ttatccaaaa tttggtagaa atcggaaaaa gtttggataa aatcgttgtt gcaacaggta
      901 atgtacatta tctgaacgaa gaagatgcca tttaccgaaa aattttgatc aattctatgg
      961 gcggagcgaa tccgttgaat cgtcatagtt tgccggatgt ccatttccga acaaccgatg
     1021 agatgctaac tgcatttcac tttttagggg aagaaacagc gaaagagatc gtagtagaaa
     1081 atacgaataa aatcgcagat atctgcgaag aagtgattcc agtcaaagat gaactttata
     1141 caccgaagat tccaggttca gaagatgaaa tatcagaatt gagttacaca aaagcaaaac
     1201 aaatgtacgg agatccgtta cctgagatca ttcaaaaacg attagaaaaa gagttgaatt
     1261 ctattaatgg taatggcttc tcggttattt atctgatcgc ccaaaaacta gtgcataaaa
     1321 gtaatgaaga tggctatcta gtaggttcac gtggctctgt tggctctagc tttgttgcga
     1381 cgatgacagg aatcactgaa gttaatccat tagcccctca ttattattgt ccagagtgcc
     1441 aatattccga gttttttgaa gatggtacgt atggttctgg atttgacatg ccagaaaaac
     1501 aatgtccaaa atgtggtgca cgcttaaaca aagacggtca tgatattcca tttgagacat
     1561 ttttaggatt ccatggagac aaagtgcccg atattgattt gaacttctca ggtgattatc
     1621 aagcagaagc ccataactat acgaaagtat tgtttggcga agactatgtt taccgtgccg
     1681 gaacgattgg tacagtcgcc gacaaaacag catatggcta tgtaaaagga tacgaaaggg
     1741 acaataatct gcagtttcga agtgcagaag tcgacagatt agcaaaaggc gctactggag
     1801 tcaaacggac aaccggccag catccagggg ggatcatcgt tattcccgat tatatggatg
     1861 tctatgattt tacgccgatc caatatccag cagacgatca aaattcagaa tggaaaacga
     1921 ctcattttga tttccactcg attcacgata atgtattgaa attagatatt ctagggcatg
     1981 atgacccaac tgttattcgg atgcttcaag atttatcagg gatcgaccca caaacgatcc
     2041 ctacggatga cccggaagtg atgcggatat ttgccggtcc tgaagtactt ggagtaagtc
     2101 aggaacaaat ttattccaaa acagggacat taggtattcc agaattcggc actcgttttg
     2161 ttcgagggat gttagaagaa acgcatccta caacttttgc agaactactg caaatttccg
     2221 gactatctca tggtacagac gtatggttag gaaatgcgga agaattgatt cgacgaggag
     2281 atgcgacact ggcggaagta atcggctgtc gtgatgatat tatggtttat ttgatccatg
     2341 ctggattaga tagcgggatg gcatttaaaa tcatggaaac tgttcgtaaa ggacaatgga
     2401 acaagatccc tgatgaattg cgagaaacct atctatcggc gatgaaagaa aataatgtgc
     2461 ccgactggta tatcgattct tgttcaaaaa tcaaatatat gtttccgaaa gcccatgctg
     2521 cagcttacgt attgatggct cttcgtgtcg cttatttcaa ggtttatttc cctattttgt
     2581 attactgtgc atacttttct gtacgtgcag acgactttga tctagtctcc atgtgcaaag
     2641 gaaaagatgc tgtgaaacaa gcaatgaaag aaatcacgga caagggatta gatgcttccg
     2701 taaaagaaaa gaatcaactg acagtgctgg agttagccaa tgaaatgctg gaacgaggat
     2761 tcaaatttgg aatgattgat ttgtataaat cggatgccgt taactttgtt attgaaggtg
     2821 atacgttgat cgcaccattt agagctgtcc caagtttggg aaccaacgtg gcgaaacaaa
     2881 tcgttgaagc aagaaaagac ggtccatttt tatccaaaga agatcttgcc acccgcggta
     2941 aagtttcaaa aacattgatc gaatatatga acgacaatgg cgtattgaaa gatctaccag
     3001 atgaaaatca attatcgtta ttcgatatgc tataagcatc tgttttcctg tctgcttgta
     3061 attttaagca gagtatggta tagttcttat aggtaatgat actaactgtg agtgagcgga
     3121 tttttcgctc actcttttta atggaattac gcacttaatt tttaaggagg cgaaactttt
     3181 gagtagcgtc gttgaaacag tcacagaaat ggtgacaccg attttagatg aacaaaattt
     3241 tgaacttgtc gaagtcgaat ttgtaaaaga aggaaaaagc tggttcttac gagtatttat
     3301 tgacaaagaa ggcggtatcg atatcgaaga atgcgccttt gtcagtgaga aactgagtga
     3361 aaaacttgat gcagcagatc cagatccgat tcctcaagct tatttcctgg aggtatcctc
     3421 cccgggagca gagcggccat tgaaaaaaga aagcgactat gaaaaggcac ttggtgatta
     3481 tgtccatgtc tccttatacc aaccagtaga tggcgaaaaa caatatgaag gatttcttca
     3541 atcatttgat gctgaacagc ttacattgaa aatacgcata aaaacacgag aaaaagaatt
     3601 gacttttgac cgaaaaaata ttgcaaaagc tcgcttagct atccaatttt aatcacggag
     3661 aggaatcaga aaaaaatgag taaagaaatg ttaaatgcgt tagacgcttt agaagctgaa
     3721 aaagggatct caaaagaaat cgtgatcgat gctttagaag cagcattggt ttctgcatac
     3781 aaacgtcact atggccaagc acaaaacgtc gaagtggaat ttgatcagaa aaaaggaaag
     3841 atccatgtct atgcggtaaa agaagtaacg gaagaagtga tggattcaca gttggaagtc
     3901 tcattgaaag atgcactttt aatcaatcca gcatatgaga tcggtgatac gatccgtttt
     3961 gaagtaacac caaaagattt tggacggatc gctgcgcaaa cggctaaaca agttatttta
     4021 cagcgtgtac gtgaagcaga aagaacgatt atttataacg aattcagtgc ttatgaaaaa
     4081 gacatcatgc aaggaatcgt cgaacgccag gataaacgtt atatttatgt caatcttggt
     4141 aaaattgagg cagttctttc taaacaagat caaatgccaa atgaatttta tcaaccacat
     4201 gatcgaatca aagtttatgt ttcccgagta gaaaatacgt ctaaaggtcc gcaagtattt
     4261 gttagccgca gccatccaga cctattgcgt cgcttatttg aacaagaagt accagaagtc
     4321 tatgatggaa tcgttgaaat tgtcagcatc gcccgtgaag ctggcgatcg ttcaaaagtg
     4381 gcagttcgct caaatgatcc aaatatcgat gcagtcggta catgtgtcgg gccaaaggga
     4441 caacgagttc aagcaatcgt caatgaatta aaaggcgaaa acatggatat cgtcgaatgg
     4501 aatgaagatc cagctgtatt catcgcaaat gcattgaatc ctgcacaagt catggatgtg
     4561 atcttcgatg agacaaatcc aaaggcctgc acagttgtcg ttccagacta ccaattatcg
     4621 ctagcaatcg gtaaacgcgg acaaaatgca cgtttagctg ctaaattgac taaccataaa
     4681 atcgatatca aatcagaatc tgacatgacg gaattttatg aaaatcaaga acaacaaaaa
     4741 gaaacagaag aactgcatga tgaagcaatc gttcagtcag atttaacaga cgatgaatat
     4801 gaaacaattg cattcaacaa tgaaacggtc gaagaaaaac cagaagctta actttagtaa
     4861 ggaggcaaag agatgaaaca aaggaaaatt cctttacgca aatccgtggt ttcaggtgaa
     4921 atgaaaccta aaaaagagat ggtccgaatc acccgttcaa aagaagggga agtatcgatc
     4981 gatccaactg gaaaaatgcc tggacgagga gcgtatgtct cacttgagcc ggaagaagta
     5041 caacaagctt gggataaaaa aattctcgat cgagtactcg aaacaacatt aacagatgag
     5101 ttttatcaag aacttcttga ctatgtcact caccaaaaag cacgtaaaga gttatttgga
     5161 aaatgaatac agttaatcgg caaaaagcca tgaatctcat cggtctagcg atgcgcgcgg
     5221 gaaaactaat cacgggtgaa gaactgacca ttgctgaaat ccgcaaacaa aaagcaaaat
     5281 tggtgtttgt ggcaaccgat gcaagtgaga atacaagaaa aaagataaaa gataagagtt
     5341 catattatga agttccttgc tttgagctat tttctgaagc agaaatcacg cagatgatcg
     5401 gaaaacctcg caaggtattc ggaataatgg atgccggatt tgcgaagaag accaaggagc
     5461 taattgaagg ttaggaaggt gattgcatgg gcaacaaaag aatctatgaa ttagcaaaag
     5521 aactcaaaaa atctagtaaa gacgtcgtgg aaaaagcaca acaacttggc atcgatgtga
     5581 aaaaccatat gggtgcgatt actagcagtg atgagaagaa attgcaacaa gcgttcaaaa
     5641 acccaagcac gaaacaacaa gctgcacaac aagcaagcag aaaaccagcc aatcaacagc
     5701 cgaacgatca acgtaaaaaa gaaacaacga acaatcaaca aaaaaataaa aataaccgca
     5761 gttatcaaga tcgcggtcaa gggagcggtc aagtgaatca aggaaaaaaa ccaacaacaa
     5821 accaaaataa tacgggaaat accagcagcc aaaatcgtca aggaacgagc caaggcggca
     5881 atcaaaaccg tactaacaat aacaacaaac caaacaataa caatcgcggc aaatttaata
     5941 ataataaccg taatcgttat aataaaaaag gtaaaaaagg caaacagcaa caatcaacta
     6001 aaccagctgt accaccacgc aaattccgtg aattgccaga tgtattagag tatactgaag
     6061 gtatgaacgt agcagatatt gcgaaaaaaa ttcatcgtga gccagcagaa atcattaaaa
     6121 aattatttat gcttggtgtc atggtcaatc aaaaccaagc actggataaa gatacgattg
     6181 aactgttagc gacggactat ggcatggaac cacaagaaaa agtccaagtt gacattgctg
     6241 atatcgataa attcttcgag gcagaagaag tcgatccaga taaattagtc tcacgtccgc
     6301 cagtagtaac gattatggga cacgtagacc acgggaaaac aaccttattg gatacattgc
     6361 gccattcacg tgtaacaagc ggcgaagccg gcggaatcac acagcatatc ggtgcctacc
     6421 agatcgatat tgatggtaaa ccaatcactt tcttggatac accaggacat gcggccttta
     6481 caagtatgcg tgcacgtgga gcaagtatca cagatatcac gatcttagtt gttgctgcag
     6541 atgatggcgt catgccacaa acagttgaag caatcaacca tgccaaagct gcaggcgttc
     6601 caatcatcgt cgctgtaaat aaaattgata aaccaggtgc aaatcctcag catgttatgc
     6661 aagaattaag tgagtatgaa ttaattcctg aagcatgggg tggagaaacg atttttgttg
     6721 agatctcagc gaaattcggc caaaacatcg aagaattgct ggaaatgatc ctgttagtcg
     6781 cagaagtaga agatttaaaa gcagatccaa cacaacgcgc aatcggaaca gtgatcgaag
     6841 cacgtcttga taaaggtaaa ggtcctgtgt caaccttact tgtccaagaa ggaacacttc
     6901 acgttggtga cccaatcgtt gtcggtaata cttacggacg tgtccgtgta atgacaaacg
     6961 atcttggccg tcgcgataaa gaagcaggac ctgcaacgcc agtagaaatc actggcttga
     7021 atgatgtacc tcaagcaggt gaccgtttcg tagt
//
LOCUS       DACIRY010000131         7036 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.137, whole genome shotgun sequence.
ACCESSION   DACIRY010000131 DACIRY010000000
VERSION     DACIRY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.137"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            542..2134
                     /locus_tag="IYE22_002338"
     CDS             542..2134
                     /locus_tag="IYE22_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9507524.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2147..2887
                     /locus_tag="IYE22_002339"
     CDS             2147..2887
                     /locus_tag="IYE22_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507525.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            3055..4404
                     /locus_tag="IYE22_002340"
     CDS             3055..4404
                     /locus_tag="IYE22_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9507526.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(4532..5122)
                     /locus_tag="IYE22_002341"
     CDS             complement(4532..5122)
                     /locus_tag="IYE22_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ9507527.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            5269..6621
                     /gene="brnQ"
                     /locus_tag="IYE22_002342"
     CDS             5269..6621
                     /gene="brnQ"
                     /locus_tag="IYE22_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ9507528.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         2100 a         1286 c         1428 g         2222 t
ORIGIN      
        1 aaattttctg ttctagttct ttttttattg tattacatac atttttgtgt ataattatac
       61 tatcgtttgc aatggataga tgattcacat tttgattgtt ttttcggtaa aagtaaagat
      121 ttctatattt atcagtattt tttcaaaaac cagcagttat agattgacaa agcctctcga
      181 gtatcgtaaa ctttttttgt ataaataact gaatacgaga tgcctcaatc aatgaggtag
      241 aggtcgcgag aatcattaac cctgtggagt gaagcaaaca tggagaagca ggaataggga
      301 ggatcgccga aatgtgcact tttttgcttg gagtgtatgt tgggacgcaa gttaagagct
      361 tgcggactgt cttagcagtg atgctaagtt gcgctatgtt ttgtgagagg tttatcacct
      421 tatggaattt tagcggcctt tttatatcta taaatcggtc gctttttatt ttttcagata
      481 aagctttacc gcatttcatc tagaccgctc gtgtatcata taatcagaaa gggagaaaaa
      541 aatgaaaaaa aatatggttt cagttgccgt actatttgct tttgtcttct cactattcag
      601 tgtgggatct ctggcaaatg cagatgaaaa aacaccgagc gatgagtttc gcgtaggtat
      661 ggaggctggg tatgcaccct tcaactggtc tcaacaaaca gatgagaata atgccttgcc
      721 aattcaaggc caaaatagtt acgccggcgg ttatgatgtt caaatcgcta aaaaagtagc
      781 agatggttta gggaaaaaat tagtcatcgt ccaaacgaaa tgggatggtc tggctcctgc
      841 ccttcaatca ggcaagatcg acgcgatcat cgccggaatg agccctactg ctgaacgacg
      901 aaaagaaatt gattttaccg atccttatta cgaatcacaa ttagtagtag tagttcaaaa
      961 agatagcaaa tatgcaaaag caaaaagttt agcagatctt tctggagcaa agatcactgc
     1021 tcagttaaat acattccact atactgtgat cgatcagata ccagatgtgc agaaacaaca
     1081 agcaatggat aatttctcag caatgagaac agctttagct tctggaatga tcgatggtta
     1141 tgtcagcgaa cgtccggaag gtgtaacggc aactagtgtg aacaaagact tggaaatgct
     1201 tgaattcagc ggtgcagatg gcttccagac aaacccggaa gatgttcaga tttctgttgg
     1261 tatgcgtaaa ggcgacccgg atatccaaaa agtaaaccag attctttctg gcatctctaa
     1321 cgatgaacga accaaaatta tggaccaagc agtcaaagat cagcctgctg caactgatag
     1381 tgaagaaaaa cctggcatca tttctgattt cactacgatt tggaaacaat acggcaatat
     1441 gttcttacga ggtgctggac ttaccttgtt catcgcattg atcgggacag ttgtcggtac
     1501 tacacttgga ttactgattg gtgtcttccg ttcggtacct gaatcaggaa atgcactgac
     1561 tcgcttcttc caaaaaatcg gaaatgcact tctttctatc tatgtcgaag tattccgcgg
     1621 aacaccgatg atggtacaag caatggtcat cttctacggt ctggcactcg cttttggcat
     1681 ttcattgaac cgaacgattg ccgctttgtt tattgtctct ctcaatactg gagcttacat
     1741 gacagagatc gttcgtgggg ggatttttgc agtagatgaa gggcagttcg aagctgcgca
     1801 agccattggt atgacccatg gccaaacgat gagaaaagtt gttattccac aagttttacg
     1861 gaatatcttg ccagcaacag gaaatgagtt tgttatcaat atcaaagata cagcagtttt
     1921 aagtgtaatc ggcgtagctg atttgttctt ccaaggaaac tctgcttccg gtgcgaattt
     1981 ccaattcttc caaacattta cgatcgtcgg gatcatctac ttagtcatga cctttacgat
     2041 caccagaatc ctgcgggcgg tagaaaagaa aatcgatggt ccttcagctt ataccaagct
     2101 tgaagaaatc gacaattcaa atttgcagga ataaggagga agaatcatga atgaaaaaat
     2161 tattgaaatc aaacatctaa gaaaaagctt cggtgaaaat gaagtattga aagatatcag
     2221 cgtaacagtc aataaaggtg aagttgtgac gatcatcggt tcttccggtt ctggtaaatc
     2281 tactttcttg cgctgtatca atttactgga aaaaccaaca ggcggcgaga tcatttataa
     2341 tggagaaaat gtattagctc ctaaatataa tttacctaaa tatcgaacga atctaggtat
     2401 ggttttccaa tccttcaacc tgttcaacaa tatgaatgtc ttggaaaact gtatgtctgg
     2461 tcaaacaact gtcctgaaac gagataaaga aactgccaaa aaagtagcaa tcgaaaatct
     2521 ggaaaaagtt ggtatggatc gttatatcga agccaaacca gcacaattat ctggtggaca
     2581 gaaacagcgt gtcgcaattg ctcgggcatt atcaatgaat ccagatgtat tgctattcga
     2641 cgaacctact tcggcgttag accctgaaat ggttggcgaa gtgcttaata cgatcaaaga
     2701 cttagcacat actggcttga caatgcttat cgtgacgcat gagatggaat ttgcaaaaga
     2761 tgtatctgac cgtgtcattt ttatggataa aggtgtgatt gcagaagaag gtacaccaga
     2821 agatatcttt gtccatccga aagaagaacg tacaaaagaa ttcctttcac gtattttaaa
     2881 tgcttaggat caatagctga ttgggacaaa gagctgcggt gtttaccgca gttctttttt
     2941 atgaattact cataaaaggt aaatttctga aaattttgga atcttagttg tgtttttcag
     3001 aaaactctct taatatggaa gtaattaaac ttttttagtt aggatgattt aaaaatgaat
     3061 gaagagcaag aactgcagcg tggactgaaa aatagacatg tccaactgat ttctattggc
     3121 ggagcgattg gaacggggtt gtttttaggt tccggaaagt ctatccagct agctggccct
     3181 tcgatattgc tcgcctatct tattactgga tgcatctgtt ttttcattat gcgtgcgtta
     3241 ggcgaacttc tgttatcaaa caccaataac cattcttttc tggattttgt tgcagaatat
     3301 cttggcaaaa aagcggcttt catcactggc tggacgtatt ggttctgctg ggtatcgatt
     3361 gcaatggccg atttaacagc catcggaatg tatgtacgct actgggcgcc tggtgttcct
     3421 cagtggcttc ctgaactgat tgcattaggt cttcttcttt gcttgaatat ggtggctgtc
     3481 tcattgtttg gtgagttaga gttttggttt gcattgatca aagttgtagc gatcattgcc
     3541 tttatcctcg taggagctta tatgatcctc acccactata cgacagatat tggcacagcc
     3601 agtatcacga atctatggtc tcatggcgga ttctttccaa ctggtgcaaa aggatttatc
     3661 ttagctttcc aaatggtaac ctttgctttt gctgggatag aattagttgg attagtcgct
     3721 ggagaaacag aaaatcctga aaaagtgttg ccggaagcta tcaataatat tcctatcagg
     3781 atcatcttat tttacttagg atctcttttt gtcattatgg caatctatcc atggaacagc
     3841 ttgaatccag ataacagtcc atttgtagaa gtattttcgg aaattgggat cacgattgct
     3901 gcttccttga tcaatttagt cgtattaagt gcagcagctt ctgcctgcaa cagtgcaata
     3961 tatagtacag gacgtatgct tcgttcttta gcgcaagaag gcagtgcccc taaaaaattc
     4021 aaaaagttaa caacacatca tgtgccaggg aatgctttga cattttctac tatcgtcatt
     4081 ttcatttctg ttatcttgaa ttacgtgatg cctagtgaag tattcacact agtaagcagt
     4141 attgccacta cttgtttctt gtttatctgg agtatgcttg tctacacgca catgaaatat
     4201 cgtaaaagta tcctcggcaa aaaagctcat tcatttaaaa tgccgttgta cccatttagt
     4261 aattatctgg tatttcttta catggccttt gtgtgtgtcg tcttgttcct aggaaaagat
     4321 acgagaatcg ctttattact aacacctgtg tggttcttgc ttctattact aatctatcat
     4381 atgaaatatg aacgtaagaa gtaagaaagt agttgtgaca gaagtggtca gcttcgagaa
     4441 ataagaagga atttacgaaa attgcttctt aaatttttgt gaaatttcgg cttatttctt
     4501 ggagataaaa gcttctgtcc cagcctcttt tctatttccc cggaaacaac cgctgataat
     4561 aggcatctgc ttttttatag gaaaaaatcc aaataccaga tacaaaaaaa cgtccgaata
     4621 acccgatcaa cggtacaaaa aagacaccgg caatcaagca taagttaatc atcatggaga
     4681 taagacttcg acggtccttt gacaaaactc tcacgacatg ggaatctggt ttatttgctt
     4741 tcatgatttg cctcatcaag ataccaaaag aaaaactcca catcgtgtat actaagacat
     4801 aagccatctc aggccaaaaa gcttcagggt tacgcccgac ccaactagta acagaaggac
     4861 ataaagtcag ccaaaagagc aaaaagatat tcgaccagac cactcggtaa ttaattcgtt
     4921 ctgccatctg aaatatacga tgatggttca cccagatcac cgcaacaaat ataaagctga
     4981 tggtataagc taataatgta tttcggatga cccataaaga atgaaagcta gatgtattag
     5041 ggatcttgat ctctaataca ataactctca aaacaatggc taccactcca tcagtaaatg
     5101 cttctaatct cgactttgtc acgatgattc ctcctcagat gattcagcgc ttttatttta
     5161 tcacttacgt tattaaataa ccatcgtttt atatagggaa ttttctgaaa attgttgcat
     5221 atttttcgaa attttgttac ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa
     5281 ttatcattta aagactatct ttttataggc tccatgcttt tcggtctatt ttttggtgct
     5341 ggaaatctta tctttcccgt tcatttgggt caagaagcag gcgctgctac cttttgggcg
     5401 aatctaggtt tcttggttac cggtatcggt cttcccttct taggggtgat tgccattggt
     5461 gtatcgaaaa gctcaggtgt atatgaatta gcaaaacgga taaatcaaac atatgcgatg
     5521 gtttttacca tcttgcttta cttagttatc ggaccgtttt ttgctttacc tcgccttgca
     5581 accacatctt tcgagattgg tttagcacca tttatcgatc ctagcagaca gacgctatat
     5641 ctagcaattt tcagtattgt attttttgtg ctggcttggt ggttttcaag aaaaccgaca
     5701 aagatattag attatgttgg aaagttcctg aatcctgcat ttttggtttt attaggtgct
     5761 ttgcttctcc ttgctttttt gaatccacta ggttctgtaa atcaagcgcc tattcagcca
     5821 aattatcaag agcatgcttt tttcacaggg tttactcaag gatataacac attagatgcg
     5881 cttgctgctt tagcatttgg gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca
     5941 aaacctgctg aaatcgctaa agacacgatc aaatctggtg cgatcagcat tatattgatg
     6001 ggaatcattt atactttgct cgcttattta ggtgctatga gcttaggtag ctttgctcta
     6061 agtgaaaatg gcgggatcac gctggctcag attgctgatc attatttagg tacttatggc
     6121 agtatcttat tagcatttat cgttatatta gcttgcttga agaccggtat tggtttgatt
     6181 acagcttttg cagaaacatt cacagatttg tttccgcaaa gaaattacgc attctttgtt
     6241 gcacttgcta gtatccttcc ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa
     6301 ttttcgttgc ctgttttgat gttcatctat ccattagcaa tgacactgat tttactagtg
     6361 ttagttggtc ctttgtttaa gcagcgtacg gctgtttatc gaatgactac ttattttaca
     6421 ttgattgctt cgatttttga tgggttgaac gcttgcccag aaactatcaa acaaacacct
     6481 attgttcaaa atcttttata tgttgcggaa agctacttgc cgtttttcaa attggggatg
     6541 gggtggatcg taccagcagt tatcggtttt gttatcggac tgatttggag tttcgctaag
     6601 aaagaagaag tagcagattg ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat
     6661 aaaaagtaat tttgaaaaat ggcttctcac atttttgcat aaatttgact taataccgag
     6721 aaactgactt ttgaacaccg tttattgaga ggtcgtgaaa aagccgctta gtttcaagta
     6781 ttaagaaata ttttgtgaaa atggcttctc acatttttgc ataaatttga cttaataccg
     6841 agaaactggc ttttgaacac cgtttattga gcgtctataa actgacctga gtattagaga
     6901 aacaaaacgg caaagtagct ccatatactt tgccgttttg ttttgaacta ataccgaaga
     6961 gaagctgatc aaacaacatt tcttttataa tatagatttt agacatatat atgatataaa
     7021 aatagttttc gcaaaa
//
LOCUS       DACIRY010000132         7003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.138, whole genome shotgun sequence.
ACCESSION   DACIRY010000132 DACIRY010000000
VERSION     DACIRY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.138"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1274)
                     /locus_tag="IYE22_002343"
     CDS             complement(<1..1274)
                     /locus_tag="IYE22_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ9507529.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQ"
     gene            complement(1415..2017)
                     /locus_tag="IYE22_002344"
     CDS             complement(1415..2017)
                     /locus_tag="IYE22_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ9507530.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2262..2468
                     /locus_tag="IYE22_002345"
     CDS             2262..2468
                     /locus_tag="IYE22_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507531.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2780..3499
                     /locus_tag="IYE22_002346"
     CDS             2780..3499
                     /locus_tag="IYE22_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ9507532.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3580..4263)
                     /locus_tag="IYE22_002347"
     CDS             complement(3580..4263)
                     /locus_tag="IYE22_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ9507533.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4481..6667
                     /locus_tag="IYE22_002348"
     CDS             4481..6667
                     /locus_tag="IYE22_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ9507534.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2578 a         1166 c         1310 g         1949 t
ORIGIN      
        1 atttgtatat aaatcgtttg agaaaaacgc tggttttcaa ttgatgtttc aaggaaaaga
       61 taaggattgg tctttgtcaa ttcatccaag ataaacaagc caaggccttc gttttttgga
      121 tttgtttttg ttgagaaccc tttttgtttc aactggttta acggtaatgt atttgttgtt
      181 gtattgatca cacgaatcat cacggaatga ttcttcttga gtatggtcaa ttcgattttt
      241 ttcttctttg cagcactagc tccttcaatc gcattgtcca agataatacc aagcatacgt
      301 acaaggttta ccgtatgttt cgtatccagt tcgattggct caggaaaatg aagctggacg
      361 atcaaccctt tttcctgtgc catcatgagt tttaatgaca gtaaactacg gatctcagga
      421 actttcatat tatccaacat acttagattg gccggaaaaa ggttcatcat ctggcgtgta
      481 ggtaatatcg tttgatcata ataagaccgt aatccttcta agtcgccttc tgaaatatag
      541 ctgtgaagag aaaaaagcag attttggtaa tcatgtttga attctctcaa gtcttgatag
      601 tttttttcta gatctttgat ataatcctgt tgcatcctga cttgttgctc taataactga
      661 tcagctaatt tcttattaga gattttccaa taataaacca agatgatcag gccaacacac
      721 agatatagaa tgaaaaaact cgtattcaaa gctaagacat caggtgtttc accagaaaaa
      781 cgggtataaa aaataatgat atagtatgta aataatatca atgtcccgat cgtaccgatc
      841 atacggcggt ccacagtgtg aaaccaagtc tctaacgatt tttttaaaca aaaagctaat
      901 aaagcagata aaaaagcgga taagacaatg tgaaaatact gcaatgaatc tcctggttca
      961 ataaccgaag atttcaaaac aaaaaaatcg ccgatgcttg ttaagtgatc tcctaaaagc
     1021 gacgtcaata acccaaaagg aagcgcgtaa accaattgtt gacgcgaagt cgaaaagaag
     1081 aaaaaagtaa gcatcagcat tcctatagca attagagcag taaaaatccc taaaatcgaa
     1141 tagacccatc caatcaaaat acaagaaatc agaatgactc ccgcaaacag atatttgtat
     1201 cggatgtgat ggtcaatcaa taagatacaa attgtgtaaa tcaaatattg caacgctatg
     1261 gaccataaaa acataaagta cagctcccat tgatttttac caaattatag cacaattggc
     1321 gtattatttt tttaattttt cagggatttt gaattcagaa ctcataccaa agcagcagcc
     1381 agatacaaaa aagtcagata agttagttaa ttgtttaatg attgtttttt tcatgaaaga
     1441 ttcctccaaa attcatcaag tagtttgtaa taggaagcaa cgtagctatt tctaaaacaa
     1501 tcccaagtag caaataggta gaaaagtaag aagctgagaa aaataacgta atgagagaaa
     1561 aaattagaaa catgctgatc gccttcttta ataatggggg acgtttctcg ggttcaatgg
     1621 gattgattgc tgtacctctt gatccatagc gaagcagtaa caaaaagcag ctgattaaca
     1681 aaaagaccat cattgggaag gagatagtca attgcagatg tttcatcacc aagggaacgc
     1741 cccataaata aagtagtgtc cacagcagac aaacgaattc agagtcggaa tgcaatccat
     1801 aagcatatct gcggatgcag atataagaaa gaaaggtgat tcctgtttcc gcccatgagc
     1861 ctgtcaccag agaaaagact agaacgaccg ctagttttcc aagattattc aataaaactt
     1921 ctaagacaaa ttttacttgg acatacacca tatcttcttc cgatttgtct ccgctgaata
     1981 aggcttctaa taatttgttc acaactttat ctttcatctc atacgtacct ccaccacaaa
     2041 ttttatgcta aaaaaaataa aaaacgtctt ttgtttcata aaaagtggtt ttgacatcaa
     2101 aaccgtcatg atctcctaaa aaaatcttcg ttttccaaga accttaccaa aaaaagtgac
     2161 agttttgata cgaaaagtac tttttatgac aagtttgtgc tttttcgagt gatttatgtt
     2221 aaaaaattac tgaccttata aaaggtgatg gagggattct tatggaaaat gaacaattgt
     2281 ttgaagtcgg cgaaacatat gcatttaaat ctgatttatt tgaatcacca ataaaaggaa
     2341 aaatagaaaa aatctatgaa aatagtgtga tgatacgtgt catgaaatgc gcagtagaag
     2401 acaatgatac cgctcataat ctgcactggc agttagttgt acgcaaatca agtacaatag
     2461 aagaataaaa acagataaaa tgttgagagc ataaagaggt tgtgagtctc ccgtttagct
     2521 ctgagttaca aggaataatg ttaaaaaaca gcttttcatg ttttatgaat attgccttgt
     2581 gaccgaggtg ttggcaggag aatactgttt atttgaaaaa agggctgcga caagactttt
     2641 gtcacagcct ttattttcat gaataggagt tacttttggc tacttttaga gaagtagctt
     2701 ttaaggagca gataaagtca aggcttttat ttctataaat gaagtagtaa aatgaagaac
     2761 agattttatt acaggaagga tgccgataat tgtgatcatt gttgcaggaa cgatcggtgc
     2821 agggaaaagc acactaactg aaatgcttgc ccaagattta gaaacaaaac ctttttatga
     2881 gaacgtggaa gataatgaag tattgccgct attttattct aatccagaaa agtatacttt
     2941 tttattacag attttctttt tgaacaaacg ctttttggca atcaaagatg ctttcagcca
     3001 tgatgacaac gtactagacc gctccatcta cgaagatagt atgctgtttc atttgaatgc
     3061 agatttaggg cgtgtttctg aagtagaagt aaagcagtat gaaggtcttt tagaaacgat
     3121 gctgaaggag ttagaagaaa tttcgccaca aaagaaacca gatctactgg tttatattcg
     3181 tgtatccttt gaaactatgt tagcgagaat caaaaaaaga ggccgggaat atgaacagct
     3241 agaacaagat cctgaactct attcctacta taaagaactc aatcgacgat acgaagagtg
     3301 gtatgaacag tttgatatat gtcccaaaat agtgatcgat ggggacaaat atgatttcgt
     3361 agccgatccg gcatgtggag aacaaatcgt acaagaaatc aagatgcggg caaagaaaat
     3421 gacagaagag gcagatgccc agtcgatccg ctacactaca aataacccga aagcaaaggg
     3481 aatctttaca actaaataat ataaaaacta agcaaattgc tcactcgcag atatttctct
     3541 gtgagcagca tcttgcttag tttttttgat tcattgagat caaatagtga accgattcgt
     3601 atgataaaat tgccataaat gaaatgtctg agtgagggcc atgatcaaaa ataaaatgga
     3661 aacgatcaaa atcgaatggg cgccaaacag atgatgacag aaaacaagga caatagctcc
     3721 tgcgataaag gcaagaacga cacttagata gtggaaaaat cgatcaaaat ctgctttttc
     3781 tttagaaata caataatcat ataagttcgt tgctacccct tttaaatttc cagttgtaaa
     3841 taaattagtg taggcacgcc cgttgatttt atcaaaagct acccactgga tcgatgcagt
     3901 aaaagcaatc aaaatcgtta ctaatgcggg atgctcatgg acgggtgtca aactaacaaa
     3961 gaaaaaaatc actgcttcat aatatagaac aaacaagcgc cagaaatgtt tttctttttt
     4021 tctaaaataa tcaattagat actttgttag aagaatccct aagaaaaaga aaagggtaga
     4081 caatactttt ttaccaacac ttagccattc tccgtcaaat gcttggatga tcgatagaat
     4141 gatattgcca gtttgagcag aggcaaaagc accatattgg atataagtat aactgtccat
     4201 ggctcctccg ataaatgtca gcaaaagtcc tattgtacgc ccctcgtgaa aggaagtctg
     4261 catttttttc atcacctttt atgaatattt ttgtcttcct tatcttagca taaacgattg
     4321 aaaaaaagga aaaattttca gaaaactaaa atcatatcgc ttcccatttt catttttaga
     4381 aaggtctgtt agtataaaga aaaaatagac aacgaaactt taaataacca acttgaaata
     4441 aacaaattca aagaagaaag atgggaagga aatcaatcag ttgaatgaaa aagaaattct
     4501 ccgattacaa aaagaattga aacgctggga gaacaagtat aaaaagcttc aacaagtctt
     4561 tcggctacaa gaagaaatca ttgctaccta tgaagcggct tatggcgaag cacccggact
     4621 tattgaggaa gaagtacaag taaaagaaag tgaaggaaac agctctttct ttaaaatgat
     4681 ggagaaacgt tcctataatc gcttcaatca agaacaaaaa gcagctatca catatgatat
     4741 gaagaaacat cttcgaatca tagcaggagc tgggagcggc aaaacgcaaa caatctgtgc
     4801 aaaagctgtt tatctgatga cgcaaaaaca agtagacgaa gaacgcatac taatgatcac
     4861 ttttacaagg aacgctgcaa atgaactaaa aaaacgagta gataatttca gccagagaaa
     4921 aaccgctgtc catatcggaa cattccatag tatctttttt aggatataca atgaaatttg
     4981 tcgaaaattt cctgaagtag caatgcaagg aatacaagga gacttttcaa aggatactgc
     5041 acaaaaagtg aatgctgttt tgcaacagct gatacgtaaa tataatcttt atatatttga
     5101 tcaatatgga gaaaaaacga ttgcttctcg gttagactat tggcagaata tgaacctttc
     5161 agtagaagaa atggttcagt tagttaaaga aaaatatgat tccattgata agaatgcacg
     5221 acagccgata tcagaacgac tgtatggatt gctcacggaa ctccaagaac aaaaaagaag
     5281 ccagcaattg ctagagttca atgatgtact ccaaaatctg aaaagcgctt tggagaatga
     5341 ggagatccag cattatatcg gccaaaaata tgattatcta ttcattgatg aatttcaaga
     5401 tactaatcca cttcaatggg aaatcgtgaa acggatgaca aaggaaaaca ggatcaagtt
     5461 gattgttgtt ggagatgatg atcagagtat ctatggattc agaggatcag agccagcata
     5521 catcaagaac tttgaaaaag aatatcctac caaaactctt ttcttattaa ctaattatcg
     5581 ttctcgggca gctattgtcc aaggagcaaa tagattgatc agctataata aaaatgatcg
     5641 gataccaaag gcgatgattc cagcacaaaa agaagcaggt gttatggaag cctatttgtt
     5701 ttctgatact aaagcagaaa gtcagtggct aatcaaacaa attcagacat ttattcaaaa
     5761 aaatgggaaa tataaggaat caatcatatt ataccgatca gcttcacaaa cgcagcagct
     5821 tgtccaatct ctgctgaaaa cgaatattcc ttttgtactg gaagcagata gtccatatga
     5881 aggtatcttt ggtatcaagt ctttccaaca tttctatcaa aaaattgttc attggcaaac
     5941 agcactgaat ctgcaaaaga aaaatcaggc ctaccagcaa ttgctccggc agctgatggc
     6001 agactgctac ctgaaaaaaa gtgaatgcga tcaatacttt tctcctaaat atcaaaatat
     6061 agccgttgca gattatattt tgtcgataag ccccaattta aaatcgaaat ctgcagaaat
     6121 ccgagagttt gaggaagcgt tgcgtaaaac agaccagcat caagaaatct attcgcttgt
     6181 tcaaagctat cttcatctgc cacgtatagc taaggaaatc gaccagagtg atcaagaatg
     6241 gatacgagaa gaagtaaagc agcatgctac tttcagaaga ctccaagcct tatcgcaaga
     6301 aacccaagcg acagctaaag aattaaaaac ccgcttgatt gcttacagac aaggaaaact
     6361 tgatgcacta tgtatccaga gcattcacaa aagtaaaggt cttagttacc gaaatgtctt
     6421 tttgattggt tgtaacgaag gaagtttgcc ttataacgga gcgacagaaa agaaagtaat
     6481 cgactttaat gaaataaaag cagaaccagt aacgacgata gaagaagaac gaagactatt
     6541 ttatgttgcg atgactagag caagaaatcg tctgtatcta tgtgtacctc aaatgaaaaa
     6601 ttcaagaaaa ttgaaagtat cgagatttat caaagaaacc gggagtcgtg tgaaaaaagg
     6661 aaagtgatag aagagagggt gtgacagaag tggtcagttt caagaaagaa gaaggaatgt
     6721 acgaaaattg cttctcaaat ttttgtgaaa tttcagctta ttttcgaaga agctatttct
     6781 ggaacacagt ttattcggaa aagggaggtg tgacaagaat tttgtcacac ctcccttatt
     6841 tattttgaaa atgagaatac tattatcaaa atagagaata tatagaaaaa ttctcattat
     6901 tcaaacgtaa ataatcagtt attttgattt tttttatatt ttattagcta ataattggta
     6961 ttttaaaaga aattactgta gaattggatt ataattaaaa aag
//
LOCUS       DACIRY010000133         6898 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.139, whole genome shotgun sequence.
ACCESSION   DACIRY010000133 DACIRY010000000
VERSION     DACIRY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.139"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1526)
                     /gene="tkt"
                     /locus_tag="IYE22_002349"
     CDS             complement(<1..1526)
                     /gene="tkt"
                     /locus_tag="IYE22_002349"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ9507535.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     T"
     gene            complement(1628..1879)
                     /locus_tag="IYE22_002350"
     CDS             complement(1628..1879)
                     /locus_tag="IYE22_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ9507536.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2026..2649
                     /gene="lexA"
                     /locus_tag="IYE22_002351"
     CDS             2026..2649
                     /gene="lexA"
                     /locus_tag="IYE22_002351"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ9507537.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            2828..3046
                     /locus_tag="IYE22_002352"
     CDS             2828..3046
                     /locus_tag="IYE22_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507538.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3334..3846)
                     /locus_tag="IYE22_002353"
     CDS             complement(3334..3846)
                     /locus_tag="IYE22_002353"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ9507539.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(3860..6157)
                     /gene="recJ"
                     /locus_tag="IYE22_002354"
     CDS             complement(3860..6157)
                     /gene="recJ"
                     /locus_tag="IYE22_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ9507540.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6299..6733)
                     /locus_tag="IYE22_002355"
     CDS             complement(6299..6733)
                     /locus_tag="IYE22_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ9507541.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(6743..>6898)
                     /locus_tag="IYE22_002356"
     CDS             complement(6743..>6898)
                     /locus_tag="IYE22_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HAQ9507542.1"
                     /translation="EPAKLAKMIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLA
                     SGIFNKK"
BASE COUNT         1894 a         1523 c         1243 g         2238 t
ORIGIN      
        1 gtctccatcg ctactttcca agcagcacgt gtttcatttc cgtctgccgg acgaatcact
       61 tgaacaccag gcatgcagcg aacagatgct agttgttcaa tcggttcgtg cgttggtccg
      121 tcttctccga cagcaaccga gtcatgtgtt aagacgtaaa caacaggagt atgttggatt
      181 gctgctaaac gaactgctgg tcttagataa tcgacaaaga caaagaatgt tccaccgtag
      241 atacgagttc cgccatgaag ttggatacca ttcatagccg aagccattgc aaattcgcgg
      301 acaccgaacc agatgttgcg tccttcataa tgttcaggag tgaaatcttt atctgcagcc
      361 accattgtat tattggaacc tgacaagtcc gctgaaccac cccagaagct tggtatagct
      421 ttcgataatt cttggatgac ttctttactt gaaacacggc ttgcttgact gcttccaact
      481 tcataagttg gcaattctgc atcccagttt tctggtagtt tgccatcaaa agcatcttca
      541 aattgttgtg ctaattcagg ataagctttt ttataattgg caaacatttc attccatgca
      601 tcttcagctt tttgtccttc ttctatcatc gtttgatgga agcgggcagc tacttcttct
      661 ggaacagtga aatctggata ctcccaacca taaacttctt tagccatctt gatgccatct
      721 tcaccaatag gagcaccatg aacagcagat gttccttctt taggagcacc atagccgatg
      781 actgttttta cttcgatcaa tgttggtttg tctgtttcag cttttgcttc ttcaatcgct
      841 ttagagattg cttctaggtc atttccgtct ttcactaaga tatgttgcca gccatatgct
      901 tcatagcgag caccaacgtt ttctgtgaaa gcttttgaag ttggaccgtc tagtgaaata
      961 tcgtttgaat cgtataaaac gatcaatttc cctagtttca tatgacctgc catcgaactt
     1021 gcttcttgag ataccccttc catcaagtct ccgtcaccgc atattgcata cgtatagtga
     1081 tccatcacat taaagttttc tttgttatac gttgctgcaa ggtgcgcttc tgccattgcc
     1141 ataccgacag ccattgcaat cccttgtcct aatggccctg tagtggcttc tacaccatct
     1201 gtgtgatgta cttcaggatg tcctggtgtt ttagaatccc attgacggaa ttgtttcaaa
     1261 tcatcgattg tcacttgata gccagctaaa tgaagcaagc tatacaacat tgctgaccca
     1321 tgtcctgccg aaagaacgaa acggtcgcgg tcagcccagt tttttgatgt cgttggattt
     1381 actttcaggt gttttgtcca taacgcatat gccataggtg ctgcgcccat cggcaatcca
     1441 ggatgtcctg agtttgcttt ttggactgct tcaatactta atgtacgaat tgtgttaacg
     1501 ccaagttggt caattttgtc aaacaaaata acccctccta ttaatttagg ttttttttac
     1561 ctttatttct tacactttca ttctataaga tgtcacacga aaaagcaatc tttttcagta
     1621 ttttttacta aaagttgtca tttcggttgt gtaagccttt tttcttttgg atttctttta
     1681 atttttccgg ggtcacgtca ttgccctcag gatcgacgac tttcattcct tcgatatgat
     1741 gacgcatacc ggaacgaaaa gcttccaaat actctttgcg taagttgatt tgttcttgtt
     1801 cttcggcttt tgagagacca ttttccttct ttttacgtgc aagttcattg atcctctcta
     1861 ttttttcagg tgacagcatg ctcagccctc cttttactct ccttcatatt atataaaaac
     1921 tgaaaaaaaa acaactagat aaactaaata aaatacgaac attcgtttga tttagcgctt
     1981 tttatatggt actatttatt tatcaagtga aagaaggtgt accttgtggc ccgacaaact
     2041 gaatcaagac aaatcgaagt cttacgatat atccatgaac aagtaaccca aaaaggatac
     2101 ccgccgactg tccgtgaaat cggggaagct gttcagcttt cttctacttc tactgttcac
     2161 ggtcatcttg cacggcttga aaaaaaaggg tttatccaaa gagatccgac gaaacctcgt
     2221 gcaatcgagt tgacaaaaca aggtttagaa aagattggga ttcgtccaac aactattcca
     2281 gtacttggcg tcgtaacagc tggagaaccc attttagcag tagaggatgc ttcagacttc
     2341 tttccaatcc ctcctaatct ttcttccgaa gaaggcagtc tctttatgct gacaattcgt
     2401 ggagatagta tgatcaatgc tgggatcttg gatggtgaca acgtgatcgt tcggaagcaa
     2461 gaaactgctc aaaatggcga tattgtcatt gcaatgactg ccgaggacga agcgacttgc
     2521 aaacgattct ataaagaaaa aaactttttc cgtctgcagc ctgaaaacga cttgctcgaa
     2581 ccaatcatct tagatcaagt atctatatta ggacgagtag taggacttta tcgtagtcat
     2641 atctattaat tgctactaat aaaagaaaac tttttaaaaa gagtttgaga cgatggtctc
     2701 aaactctttt ttcgcccggg ttcttcttct ttcaaatttt tatcacttcc ctctttacaa
     2761 accgaacaaa tattcgtata ctaaatatac gaacaattgt tcgcataaat taacgaggtg
     2821 agaattcatg aaatcaatga gacgtcttgg cttagtatta actgttttgt ttttaggtat
     2881 tattattggt aatttcggca tacgtccagc tttacttgtg tttgttccgt tatttacttt
     2941 gtggttcatg ttgtgggatg aaaagaagta ccgtcaatct gagcggcgca gaattcatga
     3001 agatcatttc tatcatgagc cctactacag atccaggcaa tcataataag gattgactgc
     3061 taattattca aaagacaaga agttaaactt ttacgggggt gtgacaaaaa tcggtcacat
     3121 ccttttttcg aataaacggt gttctaaagc cagtttttcg gtattaagtc aaattgcgga
     3181 aaaagtgatt ttttaaactt ctactggtct ttgcttgttt ccatttataa aaaaatagcg
     3241 accctcaaaa gtcagataga gaatctgact tttgagggtc gctttaaaat cgcttcttta
     3301 agaataacat caagaatagc ttcttttttc tgcttaatat tccattaatg taatgatgtc
     3361 atatcctttg attttgtcgc gtccatggag atccatcaat tcgatcaaaa atgcgcagcc
     3421 tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc gctttgattg ttcctccagt
     3481 agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt ttaatggcat ccttatgcaa
     3541 tgtcaatgta tctgttccat attctaatcc ataagtcact tcgatggttt ctcgaggtaa
     3601 cttccctttt ttgcggactg gggcaaaacc gactcctaac tcataagcaa caggacatcc
     3661 tacgatgaat ccacgggctt ctggcccaac taccatatca attcgttttt cttttgcata
     3721 atttacgatt tgcttcgtag cttcccgata agcgtcacca tctgccatca gtggggaaat
     3781 atctcgaaac acaattcctt ttgacggata gtcgggaata ctggcaatat aatcttttaa
     3841 atccactctt tgtttcctcc taggttgtta accatttctt tatggtagga atatcgctca
     3901 aaagcaaaaa ttcctcaact ttgattttat tgagccgccg ctggtataat ctgctctcag
     3961 tcaaaggatg actttctggg ctagcgactt tttgtaaaac accgttctct attgtaacaa
     4021 atttcaattc aaaaaacacc tgtatcataa aaattaataa tttttcaggg atacgcaaat
     4081 attgagctat tgcttttatt ttatgacgta tatcaattcg ttcttgagaa tggatcagtt
     4141 taaacaattt tgcgtactgt tctcttgtgc ctaccccatt caaataggcc tcatcaggcg
     4201 aaatccccat cagataaaca cgattgaatg aataagaccg aaaaacttct ttcagatccg
     4261 ctaaatcatc tggacaatcc ataataacta gagagttcgg ttttcttttt tcaattattg
     4321 cctttacaga atctagtcct tcgaaaacaa tcgcagcttc ctggataatc tcaggcacga
     4381 atttcaacgt agaagaatca aatgctaaat aaaggctacc ttccgacgtt cggctctgtt
     4441 cacggaaacg ttttgcccgc caatcaaata cttgcagacc acccacagaa aaatcagata
     4501 ccatcagctg cggtttctta cgcccgttcc attcattgat cgaaagcttt cctaccacat
     4561 ctacctgatt attcagcagt tcttcttctt gagtaccaaa accaaaagcg accgcatcaa
     4621 gctgactcga tgcattggaa aggacaaatt tcagatgctg ttggtttgct ccgattcttt
     4681 tgatattttc tgcttggact tgtcgaaata aaaactgagg caaaggatta tccgtaccaa
     4741 atggcgccaa tagtttcagc tcatcaattc gctctactgt tacttcattt ggcagcaata
     4801 cttcatcaat tctcaactca ggccctctca tcaagtcaat ttgatgatcg actatatatt
     4861 ggttcatctt ctcctgtagt atagtgacat tttcacttgg catcgttaaa ccgactgctg
     4921 catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg gtcaagcatt tcaaaaagat
     4981 tcaaggcttc aatactacgt ccagatcctt tagctgatcc atcttctttt aaagtaagga
     5041 caagcgttgg tttacccgtt tcattcatta ttttcccagc aacaatgccc aaaacgcctt
     5101 catgccatcc tcggtttacc aatagatgga tatgattctc cttattgacc atcgctaatg
     5161 cttcttgtgt tatctgctct acgattgctt ttctttcttc attgatctca ttcaattttt
     5221 ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag taacgagacg gcaggacgtg
     5281 gatcgcccat ccgaccgatt gcatttagtc tgggagcaat cgaaaaaccg atcgttgttt
     5341 cgtctgcgtc cctcattttt acgccgcttt cttcaaatag cgcctgaagt cctatccgtt
     5401 ctgaatgatg aatggcattg attcctaatg aaacaaggat tcgattctca tcagtcaatg
     5461 aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa aaattctaca ggcggttctt
     5521 ccaataatgc agaagctact ttgaatgcta ctccgactcc tgccaaatct ggaaatggat
     5581 agtgccccgc tggatgacga gggtgaacaa tcgcataagc gtctggtaaa ttctcaggta
     5641 gttcgtgatg atctgtaatg atcacgtcta caccagcttg ctgagcgtat gctaccgcat
     5701 catttcctgc taccccgtta tcaacagtga tgatcaactg gattccttct tcgatctttt
     5761 cttggtatac agattggttt ggtccatacc catgctcaaa tcgatttggc aggaatactt
     5821 ctacttctgc accaagtaat tccagtgttt ctttcattac cgttgcactg gtgatcccat
     5881 ctgcgtcata atcaccgtaa acgaggattt tttctccact gattacagct tcttggattc
     5941 ggctgattgc tttatccata tcgtgcatca gatacggatc atgtaattct tgttcctttg
     6001 gatgtaaaaa tcggttgata tccttttcct catggtaacc tctttgccac agtaacttac
     6061 cgacaaatgg agataatttc agttcacgga tcatttggag aaaggattca gaaggctctg
     6121 tctcgcttaa cttccaatca aaatttgctt gtctcacttt ctcactccta acttagttat
     6181 tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga ataaaaacag atcagatccg
     6241 ctagacagat gttcagcagg acctgatctt ttgtacgacc atttatgaaa tatccctatt
     6301 agtcaaaata attgaagctt tgtttgggcg attgagtttt ttgttcttcc ggcgctttca
     6361 attgactgat ttgtgcggtt ttcgcttgta actccgcctg ataagcagct tccaactccg
     6421 cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc ttcttcaatt ttcttttgaa
     6481 gttctgtttg atattctgac atttcctgag tcagctgttt gatttgcttt ttttgctgcc
     6541 atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc aatcagcgca gaaccgatga
     6601 tcactaagat caatggcgca gaaaaggaag tgaagccaaa acttacagga acatttttat
     6661 tgttcaatac agcaaaaaga acaataatca aaactaaaac aaatccaaga atcacattgc
     6721 tttgcttttt caattccttc acctactttt tgttaaaaat tccgctggct aaataatcac
     6781 caatcgttgg aaacagcgta taaaattttg atgcaacttc taaaataaac ggttgattga
     6841 tttcccgttt tggatgtctc atattgcgga cgatcatttt tgccaacttg gcaggttc
//
LOCUS       DACIRY010000134         6738 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.140, whole genome shotgun sequence.
ACCESSION   DACIRY010000134 DACIRY010000000
VERSION     DACIRY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.140"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(654..1052)
                     /locus_tag="IYE22_002357"
     CDS             complement(654..1052)
                     /locus_tag="IYE22_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507543.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1268..2323
                     /locus_tag="IYE22_002358"
     CDS             1268..2323
                     /locus_tag="IYE22_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ9507544.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2343..2696
                     /locus_tag="IYE22_002359"
     CDS             2343..2696
                     /locus_tag="IYE22_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507545.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2878..3174)
                     /locus_tag="IYE22_002360"
     CDS             complement(2878..3174)
                     /locus_tag="IYE22_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507546.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3178..3996)
                     /locus_tag="IYE22_002361"
     CDS             complement(3178..3996)
                     /locus_tag="IYE22_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9507547.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4222..4443
                     /locus_tag="IYE22_002362"
     CDS             4222..4443
                     /locus_tag="IYE22_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ9507548.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4445..4711
                     /locus_tag="IYE22_002363"
     CDS             4445..4711
                     /locus_tag="IYE22_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ9507549.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4824..5363
                     /locus_tag="IYE22_002364"
     CDS             4824..5363
                     /locus_tag="IYE22_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507550.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
     gene            5572..5754
                     /locus_tag="IYE22_002365"
     CDS             5572..5754
                     /locus_tag="IYE22_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ9507551.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            5857..6141
                     /locus_tag="IYE22_002366"
     CDS             5857..6141
                     /locus_tag="IYE22_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507552.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6157..6393
                     /locus_tag="IYE22_002367"
     CDS             6157..6393
                     /locus_tag="IYE22_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507553.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6415..6669
                     /locus_tag="IYE22_002368"
     CDS             6415..6669
                     /locus_tag="IYE22_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507554.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
BASE COUNT         2337 a         1133 c         1061 g         2207 t
ORIGIN      
        1 aaaatttaaa tgttttaata ttacaaataa aatcatgtta tcgtaaaatg gtataaaaaa
       61 gctaaatatt ctatttttgt tattttatta gcagggaaat ttaaatagtt tacaagaatc
      121 cgtagaaata tattggtttt gccttcagat taatactgga tcatattaat ctgaatataa
      181 tatatgaact aataaatagc ttaacctcgc tattttgttt tttctgatca ctatctatac
      241 agcattagta aatagaattt taaaaataaa aaaattaaga caaaaaaagt atcaacataa
      301 acatatacat atacacgttt atgttgatac acttaatttg tcctttaaaa agcacttatt
      361 ttaatattat gttattttat aatcttatta caagctgatt gttagacgtt gtcctttcat
      421 tacatttatt aacggttatt ttatcgttta atttattaat tatttttgat ctaaaactta
      481 gcttaacaaa taaataatta accaaccgcc agataaaaat gcgatcattg ctaatacata
      541 agcaaagata aaaatatgtt cgatcagctt aaaccctgat gcgattaagc agaccaagta
      601 aaatagaaat tctcttacag ataatggtaa ttttctaact agcatactca taattaacac
      661 cccaaagtaa tattgtattt tgaacatttt acatccaata cagcatcaat caaccattgc
      721 caatcaaaaa tacaaggctt cattgaagta tgtttttcag atgaaaagat ataatataat
      781 ttcttcggaa taatattttc agttgttggg atcaagctaa atttaatacc atctaactca
      841 tagctaccag ctttctctcc attaaaaatt tccttagcac tttcttctaa agaatcaaaa
      901 tatttttcga cagcaacctt tgaagctttt actattgaag catttccctt ttcgataaaa
      961 tctgcttttg ttttaccgac aataagtaat tcttgatcag taaaatcttc taaaataata
     1021 gtagtgtaat tcttatttct tctcaatcgc atcaggatca ctccttaata taatttatgc
     1081 ttttataata tcataataag ataaatttgc aagtgtttat ttacaccttt ttatttacac
     1141 tttattgtaa ttgattatac ttttttgatt gcattttttt cttataaaca gttgcaacta
     1201 ttagtaaata ggtgtaaact attttcgtac cgaaatacca taaacacaaa cagaaagagg
     1261 tcataccatg aaagaaccat tattgttttc cgttgactta gggaataaat cagtaaaaga
     1321 aatgagtaca cacgtttctc cactctcgct tccctctcgt attttgaaca aaaatatcat
     1381 taattcagga cgtttttcta atataggttc ctcattaaaa cgatcactaa acgtttctga
     1441 atatagccta gtaaacacta ctgaaagcta ttattttggg aaggatatac acgagctagg
     1501 aaaagatgac tacgtgatag aatcactagg ttttgggatc agcagataca agcaggaaac
     1561 ctaccaagat atcctatctt tctctttagc taacctagca cgtttagaag atcaatctga
     1621 tgacgtttta accgttaatc tagttttagg tctgccttca tccgactata ccgaaacaat
     1681 cgtcaaatac gtgatgcaac aagccacacg acaacattca gttgaagttg acggtaaagc
     1741 ttataatata aacgtagata ccgtttgggt actgccacag ccgattggaa cactatataa
     1801 cctcctcctt gctgatgatg gtagtgtaca agatgaatca ttgatcagtc agaagatcgg
     1861 ttttgttgat attggtggtg gcacgaaact ttttgatgtt gtccaaaatt ttgaaattga
     1921 cgaaaacaac cgtgaacaaa aggataccgg tatttttact ttatataatc gtatcgtaaa
     1981 tatgcttgac cttccgatca gtcctaatgc tttccaactt gaaaagatta tccgagaagg
     2041 caaagaaaaa ggcgtatatc tatttaaacc aagcggaaat gaaagttttg atatttcaga
     2101 agtagttgaa agagaaataa aaacatacac caatatggtt gttgcagaag ttttagctac
     2161 attcaaaaac ttcacaacct tagataaagt tgtctttact ggtggtgggg cttctattat
     2221 cgacaaacaa cttgtaaaag ataaaattcc gcataccttg tttgttgaag acagtgaatt
     2281 ttctaatgta aaaggctttt acaagtttgg tttaactcaa taaaacagcg aaggagatca
     2341 acatgaataa gggaaaatca gagcgttatg tagttataat caatagagaa aaggttagcg
     2401 agttaccttt agcagagttt cttgaatctg aatatgaaac actaggcgtt aaatactctc
     2461 agacgatcaa aaaagctttg aatctatatc ggcgtgtaaa gaacaatgaa gttacaatta
     2521 ttgaagcagg caacgataga aacgatagca aagtagctga agtaaagaat aaaccaactg
     2581 atcataaccc tattcaacaa gcgcatcctt caacggttac atccgatcat tcagcaaaga
     2641 aagaaaagcc aatgctaaaa gggttcaatg caccaagtaa caaagactta ctttaaaaat
     2701 aaaaaggcga ggttacaata cctagccttt ttattatgta caaatacaaa cataaacata
     2761 tacgtataca tgtatataaa catgtatacg tatatatcca taaacacgta tacgaaattt
     2821 agttaagcta tacctaaccg cttattccaa taaaaaaagt gatcagcttt aaactgatca
     2881 ctcaccaaaa acttcatcat acaagttatc tatcatttca ccaagtaaag ctgaatctga
     2941 tcgaattcct aacttttcgc tgattttaga tagtttcttt ctttgtgatg gttgaagcgt
     3001 gaattgataa tttttcttct tttcagaact atcacttttt agtttagcta ccaaagaact
     3061 tgttgtttct ttaggtgttt gtttaggttc ttcaactgct tcaaccactt caggaacttc
     3121 ttctacttca gcttcagcga attttttaaa tctattttct ttcacttcaa tcactcctta
     3181 ttcttgtgcc gtatcaatta ttttgttgta tagcgtaaac gtgctttcaa agaaatcctt
     3241 attcttacga tagatattct tatccttagc gaactcagca atattcttac cttctaagct
     3301 agttgcatta agcaattcct tctcttgtac atatccaaga taattagata acgttggtac
     3361 caattctagg aactcacgac ttgaattagt attatgtttg atcctgctac cgatcgtagc
     3421 gtatttagtt tccatcagtg tttcaccagt cttaatatca atcaactgtt ttgctagtgc
     3481 atcaatatct ttaccaagct taagcaaagc ttcaaaacca tctttagatg gatcagaaat
     3541 gcctacgaca agatcagata cagcccaagc attttgagct agtagacctg catcattatg
     3601 agtatcaatc agaattagat catacttctc tttaagctgt tcatagttaa caacgatcca
     3661 tctaaacagt agggttctgt tgttaggcat atcacgcatt tgcatctcaa cttgttttaa
     3721 atcttcacct gctactaaaa tatccatgtt ctcatttata gctaaaggtt cagctgatcc
     3781 atgaagatat aaatgattga ttgtattctc tcttttcgct ttactgaaag catcaggaaa
     3841 tcgttttgat aggttttgcg acttatcttc atcaatatac agcaccttca tacctaaatt
     3901 ttcgacagca tatccaccta gattgtagat tgttgttgtt ttacctgttc cacctttttc
     3961 aatgtttcca gtaatgattg acaacttctt ttccatgatt aaacctccct tattaatctt
     4021 ttgtaactcc attatacact ataaacacgt ttatgtatat atttatatac ataaacgtgt
     4081 ttatatttat atttgctttt atattctaca acataaacat atatatgtat atgtttatgt
     4141 ttatgtatat attcaaatat ataactattg catttgtcat acaatcgtag tacaatgtaa
     4201 ttgaaaagga gttgataaac tatgccagta ataagtattc gtgtatctaa agaagaaaaa
     4261 gattttcttg aaaaagttgc tgaattcaac ggagataaca tttctgaatt tatgcgtagt
     4321 aattccctgc aaacagcaga aacgttagta gactttgcca cctacaagca actaatgaaa
     4381 gagcatgagg aaaacgatca aagtatttcc cacgaagata tgctaaaaga gttaggctta
     4441 taagatgact taccaagtaa gatatgaacg tgaagctcaa aaagcgctaa aaaaaatgga
     4501 taagtttcag gcaaaggtta tcctaaactg gatcgagaaa aatcttgtag gcactgatga
     4561 tccaagaaag catggtaaag gtctaacagc taataaatca ggctactggc gatatagggt
     4621 aggtgcatac cgtataatag ctgatattag cgagcatgaa gtgacaattc ttatattgtc
     4681 tattggtcac agaagtgaag tttataagta acagacaagc tgataaggct tgtctgtttt
     4741 tttgttaacc caacaaaatc actgtttacg atcccacttt acctaaaaaa gaacaatatc
     4801 ctcttgatat tatacacacc agtgtgtata atataagtgt aaacaagcta attaactttc
     4861 aaaaggagcg attttttatg ggatggtatg gtgattattc atcagcaaat gaagtgatcg
     4921 aagaaaagaa agggtacgcc aaaaatggtt tggaaatcgt aggtaccaaa gtaacgaaga
     4981 cctatggaat tatccttttc aaagatgcag aaggaaccta cgatattgac agcttcatct
     5041 ttcgagatga aatgtacaaa ccaatctact tcttaaatga cagtcctgct tactttaaga
     5101 aaattccgaa aaaatggttg aaattctttg tcggtgatca agaaaaaatg gttaagctac
     5161 aactagatag aattaaagaa gaaagcaata aaccaaaact tgatgactta ttagttgcag
     5221 gtaaaactta ctcaatctgg gatggatcgt atgtcgctgt ctatcagtat aaactcaaac
     5281 gtagctttat ttttcgacta caagatggat cactcactcg gttcactcga ctatcagcag
     5341 gagatgttaa ggaagtaacc taaaacttaa atacataaac ataaacgtgt atacgtttat
     5401 gtttatgtat atgtatatga atataaacgt attaggtacg aacactttta aattcttttc
     5461 tgggctttgt ttttagggta gggctaaaaa aaacatcacc atctgatcca aaacagctca
     5521 gaaacgcatt taaacgtatc tgataccaat attacagaag gagtgtatcg tatgattaac
     5581 ctatttcttt tagtgacagc tagtacatta ttctttactg gaatatcact gataatttcc
     5641 tatttgatct ataagaaagc cgaaaatcac acaagcaaag caatcggact agccgtttca
     5701 tcctttttaa ctttgctgac tttatttgtt actttatcgt tttcttttgt ttagaacagc
     5761 tgatccatta gcaccttttt ctctttacat tatacacact gttgtgtata atgtatttgt
     5821 aaacaagcta attaactttt aggaaggagt gacaagatgc taacagctac tttacagcaa
     5881 cctactcacg ttgctattgc agaaagttat acaattaatg cagggatcgt taaacagctg
     5941 atcaaggatc gagaattcaa aaagtttaac agtcggcaag cgtataacag acagctaaag
     6001 aaagctattc taaagcacaa agattccttc atcgggaact tggaaagtgt agatgttaaa
     6061 actgtttcaa cggcattttc agttgttgaa gtgactattg acggtcgctt gatttctcta
     6121 tttgcttttg gaattaattg atagggagtg tgaactttgg aaggtaagac ttttgcagaa
     6181 atttggactg aatttattga ggtaccaact cgcattgatc cgataacttc tgaaaaggta
     6241 ttggatgcag acttctacaa tctcaaagca ggtactagcc ttgaatgcgt ttggatgtat
     6301 ttagaagagc atttcaaagg cgtaacgctc ggtcacgtct tataccacaa tggtgccgag
     6361 tgggatgcaa ttggaaatat ccatttttct taatataaga aaggagaaca caatatgaat
     6421 tataccgatg aagaacaaat cgctttggaa tacgttttca caaatgaaga aggggaggaa
     6481 attgaaaaag tcttgatcat ttcatcaaaa gacttggaga aggctttggc ttatgaacca
     6541 tacgaatcaa ccgtaactgt cattgaattc ttagatacct atacttctaa tgatacttgg
     6601 gaaattatga atatttgcaa cgctaataac attcaatttg ctgaaaaaac caataaatca
     6661 ttttattagt tttgcaataa aacatcaccc gtggtgctag ttaattttaa tatcctaaag
     6721 aatatctcaa agttcctt
//
LOCUS       DACIRY010000135         6600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.141, whole genome shotgun sequence.
ACCESSION   DACIRY010000135 DACIRY010000000
VERSION     DACIRY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.141"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..276)
                     /locus_tag="IYE22_002369"
     CDS             complement(<1..276)
                     /locus_tag="IYE22_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9507555.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            462..1397
                     /locus_tag="IYE22_002370"
     CDS             462..1397
                     /locus_tag="IYE22_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9507556.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(1482..2492)
                     /gene="gap"
                     /locus_tag="IYE22_002371"
     CDS             complement(1482..2492)
                     /gene="gap"
                     /locus_tag="IYE22_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ9507557.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            2780..4093
                     /gene="obgE"
                     /locus_tag="IYE22_002372"
     CDS             2780..4093
                     /gene="obgE"
                     /locus_tag="IYE22_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ9507558.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            4314..4901
                     /locus_tag="IYE22_002373"
     CDS             4314..4901
                     /locus_tag="IYE22_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ9507559.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            5078..6019
                     /locus_tag="IYE22_002374"
     CDS             5078..6019
                     /locus_tag="IYE22_002374"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ9507560.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            6016..>6600
                     /locus_tag="IYE22_002375"
     CDS             6016..>6600
                     /locus_tag="IYE22_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ9507561.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDK"
BASE COUNT         2103 a         1145 c         1494 g         1858 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgggttataa aaatgaacat tgcatagtag agattctttg attatactaa
      421 ttcaaggtta tatgaaaaaa gagaaatgag atggatgatt tatgaaactt tggatgaaaa
      481 tccttttagg cgtcgcttta gctctgattg ttgcgttagt gtttgcaggg aattattttt
      541 ataattatgc tgttgtgcct tctgagaaag actttttaga cgaagataca cctggtacaa
      601 cacagacatc caaagaaacg gatgcacata aatggtttag ttcaaaagaa aaccgaagcg
      661 attggaccat cacttcaaaa gacggtctga aattatcagc gatttatctt cctgctgaaa
      721 cgaaaagtga aaaaacagct cttgtggctc atggctatat gggaaacgct gaaacgatga
      781 ccaattacgc aaagatgttt catgatatgg gttacaatgt ccttgttcct gatgcaagag
      841 gacatggaaa aagcgaaggg gattacattg gatttggatg gccagagcgt aaagactatg
      901 tacagtggat aaacaaagtg ctagaaaaca atggaaaatc gcaagaaatc gttctatacg
      961 gtgtcagcat gggagcagca acagttatga tgactagcgg agaaaaacta ccagataatg
     1021 tgaaagcaat cattgaagat tgcggttatt cttccgttca tgatgagctt gcttatcagc
     1081 tagacgatat gtttagtctt ccagcatttc cgcttatgca agtaacgagt ctagtgacaa
     1141 aagtccgtgc tggctacttt ttcggtgaag caaatgcagt ggaacaattg aagaaaaacc
     1201 agcgcccaat gctctttata catggagacg aagatacatt tgtaccattt gaaatgttag
     1261 ataaggtcta tcgtgcaacg aagggaccaa aagaaaagta tgttgtaaaa ggagccgagc
     1321 atgcggaagc atacaagaca gatcctgaaa aatatcagca agtagtccaa caatttttaa
     1381 gccaatatgt tcattgaacg agtttaaaag taggggatga aaagaaagaa gcctttgact
     1441 ggatccagtc aaaggcttct tttatcgatt gatctatcaa attacaagtt agcgaatttt
     1501 tctagtaagc gaatcatttg gcaagtaaat ccatactcat tgtcatacca tgcaacagtt
     1561 ttaacaagtt ggaaatcacc agcagttgtt acttctgttt gcgttgggtc aaagatcgaa
     1621 ccttgagttg ttccaatgac atcgctagaa acgatttgac ggtcatcata cccgaatgaa
     1681 ggattgtcaa ctgtgtgttt tttgattgct tcgtttactt gatcagcagt tacttttgtt
     1741 ttcaatactg aaaccaattc agttaatgaa ccatcaacga ctggaacacg ttgtgcgtgt
     1801 ccttgtaatt ttccttgtag ctcagggata actaagccga ttgctttcgc agcaccagta
     1861 gaatgtggga tagtattgtc agcagctgaa cgagcagcac gcaaattacc gccttttaca
     1921 ggtccatcta agagcatttg tgtggaagtg taggcatgaa ctgttgtcat tgtaccaact
     1981 tcgatcccaa attcattatt taagaaataa gccataggtg ctaaacagtt tgttgtacat
     2041 gaacctgctg agatgatttt gtcgttttca tctaatgtat catcattgac gttatagacg
     2101 atggttttca tagcaccagc tggggcagaa ataactactc gtttgacacc agcatctaaa
     2161 tgcgcttttg ctttttcttc agatgtataa aaaccagtac attctagtac gatatctaca
     2221 ccgttttctt ttgcccaagg gattttgctt gcgtcgggtt ctgcataaac acgagttgct
     2281 tcgccatcca ctacgattga gtcttctgtt gcagtaactg tgccaggata agtaccatga
     2341 gtagagtcaa attgcaataa ttgtgctaac atagttggac ttgttaagtc gttgattgct
     2401 actacttcaa tatcatctga gacttctttg atgcggcgga aagccagacg gccaatacgc
     2461 ccaaatccgt taatacctac ttttactgtc atatcaaaag ttcctccttt attaggggtg
     2521 ctgtgaaacg gcatcctcaa ttaatacaat taatacactg ttattctgct acgcaaagaa
     2581 tattttgtca aaaaattata cttggtagca gaaaaaaggt tggaaacctt accaaatgat
     2641 aaaaaaagaa gaaggtaaac aatctttttg ctgaaatgat agacaaaatc agatttcacc
     2701 gataaaataa gtaagacttt tgtttattgt ggaaattcag gcagtcaaat aaataaatga
     2761 ataataggag gacttaatta tgtccatgtt tttagatcaa gtaacgatcg atgtgaaagc
     2821 tggcaagggc ggagacggaa tggttgcatt tcgtcgcgaa aaatacgttc ctgacggcgg
     2881 accggctggc ggagacggtg gtcgtggcgg cgatgtcatc ttgatcgtcg atgaagggtt
     2941 acgtacattg atggattttc ggttcaatcg ccatttcaaa gcgcagccag gtgaaaatgg
     3001 gatgagtaaa gggatgcacg gtcgtggttc agaagatacc tatgttaaag taccacaagg
     3061 aaccactgtc cgagatgcgg agactggtgc tcttttagga gatttaattg aaaatggtca
     3121 aacactagtt gtggcaaaag gtggacgcgg tggacgaggg aatatccgtt ttgcctctcc
     3181 tcggaatcct gcaccggaaa ttgctgaaaa cggcgaacca ggacaagagc gaaaaatcga
     3241 actagaattg aaagtattgg cagacgttgg acttgtaggt tttccatctg tcgggaaatc
     3301 gactttacta tccgttattt catctgctcg tccaaaaatc ggcgcttatc atttcacgac
     3361 gctcgtccct aatctaggaa tggttactac aagtgatggc agaagttttg ccgcagcaga
     3421 cttgccaggc ttgattgaag gagcttcgca aggtgttggg ctgggtacac aattccttcg
     3481 tcatatcgaa agaacacgag taattttaca tgtgatcgat atgagtggga tggaaggacg
     3541 agatccttat gaagactatc ttgccatcaa caaagaatta tctacttata acttacgctt
     3601 gctagagcgt ccgcaaatca tcgtggcaaa caaaatggat atgccggatg caccggaaaa
     3661 tctagtgaaa ttcaaagaac agctgaacaa ggaaaaagaa gatgaatttg ctgatgacat
     3721 cccagtattt ccaatttctg gagtgactcg acaaggattg gacgcgttat tgaatgctac
     3781 agcagatttg ttagaagtaa cgccagaatt cccattgtac gaagaagaac tggaagaaga
     3841 aactgtacac tatggattca atccagaagg tcctgagttc caaatcgatc gtgattcaga
     3901 tgcaacgtgg atcttatctg gtgagaagat cgaaaaatta ttccaaatga cgaatttcga
     3961 tcatgatgaa acagtgatgc gttttgctcg tcaattacgt ggtatgggtg tggatgaagc
     4021 tcttcgggca cgcggtgcga aagatggcga ccttgtacgt atcggtgagt ttgaatttga
     4081 atttgtcgaa taggcaagca aataaaaaca aataccaaaa aatatagatt cagatttaag
     4141 ggaggttgtg acagaagtga tcagcttcaa gaacgccgtt tattcggaaa aaggagctgt
     4201 gatacgactt ttgtcacagc cctattttat tggaaaaaaa gagtgctttt cttttcttat
     4261 tttacaatta aaggtaaaat aaataacaag aaggtatgaa aggggcgttt ttaatgagca
     4321 aacgagtaat cattttaaat tttcaagtag aaagccaggc atatcaggca ttttcagaaa
     4381 tcaaaaagtt acatgctgtt cgttcgatta aaggagaaca gatggcagtt gtcagccatg
     4441 atcctaatgg gacacatcaa ttcaaaatag aagattttat cgactttacc ggtaataaca
     4501 aatcttctac gggtggatta atcggaatgt tggttggtgt attaggtggt ccgctaggga
     4561 ttttattagg atggtttgcc ggaggaatga tcggtgcgac acaagatgcc aaagaaataa
     4621 aagatgccca gaccgtgttc gactttgtag gaaataaaat cggtgaagga gctacaggac
     4681 tgatcttgat tgctgatgaa gaggacaatc gcccactgaa tcaattggta atgatggaat
     4741 taggcggaga aattacccgc ttggattatg aagatgtcga aaaagatgtg catgatgcga
     4801 aagaagctga aaatcatgcg aaagaagctg cacaaaagac ttggaaggac aaacatccgc
     4861 aaacttccaa cgacaatgaa agttctggat cttcaaatta ataaaaaaca gcagaggatc
     4921 tcaaacgggt tcgctgctgt ttttatagta attttcaaag aaaacgctat cgctttagaa
     4981 cgttattttt aatttgaacc cattctcgac ttacggttga ttatcccttc tttttaccct
     5041 aaaattaaag aggaacttaa agaaagtcag gtagattatg gaattacaat ttttaggaac
     5101 aggtgcaggt gttcctgcaa aacatagaaa tgtgacaagt atagcattga aacttttaga
     5161 cgagcgcaat gaagtttggt tgttcgactg tggcgaaggc acgcaaatgc agattttgcg
     5221 aacgactatt cgtccgagaa aaatcggtaa aatttttatt acacatttac acggcgatca
     5281 tatttttgga cttcccgggc tcatcagcag ccggtcattt caaggtgggg atacgccatt
     5341 agagatttac gggccaaaag ggatagagga atacataaaa gtctctttag gtatttcgca
     5401 aacgcgttta agttatccgc tgaaattcat tgagctaaat gaaacagccc cgatttttac
     5461 tgatcagcaa ttttctgttt atacaaaaaa actaaatcat gggatcgaca gttttggtta
     5521 tcgtgtggtt gaacatgatc ataaaggaga attgcaggtg gatcgtctaa aagaattagg
     5581 tattccagct ggaccactgt acggcaaact gaagcaaggt gaaacgatcc aactagaaga
     5641 tgggcggacg atcaatggaa aagatttcgt aggtccagat aaaaaaggac gaatcgtgac
     5701 tattttaggc gatacgagaa aaacacataa ttccgttgtg cttgcggaaa atagcgatat
     5761 tctggtacat gaaagcacat ttaataaaga tgaagcaaga atggcgcaca actatttcca
     5821 ttccacgact catcaagctg ctgaagttgc aaaagaagcg caggcaaaac ggctgctact
     5881 tactcatatc agtgcgagat atttaggaaa agcagcttta gagcttgaaa aagaagcaca
     5941 agaagtgttt gaaaacacga cgatcatgaa agattttgat agcattgaga ttccatttcg
     6001 cgaggaggaa gaggcatgaa tttagaagat aaagttgttt tagtaacagg tagttcaggc
     6061 ggtctgggcg cacaaatctg ttatgaagca gcaaagcaag gagcaatcgt tataagttgc
     6121 gcacgaagaa tggcatttgt agaaggtgtc agagacgagt gcagacgctt gagcggcaaa
     6181 gaggcttatg ctttcaaggt agacgtcagc aatcctgaaa gcgtggacga attatatgaa
     6241 aaggtgatgg aagaagtcgg gcgcgtcgat attctggtaa acaatgcggg ttttgggatt
     6301 ttcgaagact tccttacatt cgaccttgga aaagcatacg atatgtttga agtcaacatc
     6361 ttaggaatga tggttttgac acaaaaattt gcgatcgata tggcagaaag aagacaaggt
     6421 catatcatta atatcgcgtc tatggctgga aaaatggcaa ctgcaaaatc aacggtttat
     6481 tctgccacga aatttgcagt ccttggcttc tccaacgctt tgcgattaga attgaaacca
     6541 cttggcgtag ctgttacgac agtgaatcca ggtccaatcg agacagattt cttcgataag
//
LOCUS       DACIRY010000136         6496 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.142, whole genome shotgun sequence.
ACCESSION   DACIRY010000136 DACIRY010000000
VERSION     DACIRY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.142"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(264..2390)
                     /gene="pnp"
                     /locus_tag="IYE22_002376"
     CDS             complement(264..2390)
                     /gene="pnp"
                     /locus_tag="IYE22_002376"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ9507562.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2578..2847)
                     /gene="rpsO"
                     /locus_tag="IYE22_002377"
     CDS             complement(2578..2847)
                     /gene="rpsO"
                     /locus_tag="IYE22_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ9507563.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            3098..3661
                     /locus_tag="IYE22_002378"
     CDS             3098..3661
                     /locus_tag="IYE22_002378"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ9507564.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3914..4435)
                     /locus_tag="IYE22_002379"
     CDS             complement(3914..4435)
                     /locus_tag="IYE22_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ9507565.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4438..5280)
                     /locus_tag="IYE22_002380"
     CDS             complement(4438..5280)
                     /locus_tag="IYE22_002380"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ9507566.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5273..5998)
                     /locus_tag="IYE22_002381"
     CDS             complement(5273..5998)
                     /locus_tag="IYE22_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ9507567.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6174..6470
                     /locus_tag="IYE22_002382"
     CDS             6174..6470
                     /locus_tag="IYE22_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507568.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         1710 a         1379 c         1238 g         2169 t
ORIGIN      
        1 cgagggacag ctgattgaac atcgtttatt ggagaggttg taaaaaagct gttctgcatc
       61 aagtaataag aacagccaag taaaaacagc tcctaatgtt tttaacttgg ctggacttat
      121 taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaggactg tgacacggct
      181 tttgtcacag tccccttttt tcgaataatt attgtccccc tgtcaccttc acgatcacaa
      241 gtacattatt cctatgaatt aaattatttt tcttcatttt tcgttgaacc atcttcgttc
      301 aacattgctt ttcttgacag atttacgcgt ccttgtttat cgatttcagt tactttcaca
      361 aggatctcat cacctagttt aacaacatct tcgactttat tgacacgatc gtttgctaat
      421 tgggaaatat ggacaagtcc atcttttcct ttgatcaagt tgacgaatgc accgaatttt
      481 tcgatacgga caactttccc tagataaact tcaccaactt tgacttcttt ggtcaaatct
      541 tcgatgatct tgatagcttt tttgatcatt tctgcatcag ctgaagcaat acttactttt
      601 ccatcttgat cgatatcgat tttcacacca gtttcatcga tgattccgtt gatcgtatcg
      661 ccgcctttac cgataacatc tttgatttta gcaggttcga tctggatcat ttcgattttt
      721 ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg ttgaagtcaa ttcttctaag
      781 atttccatac gtgcttgttt tgcttgagtt aatgcttcag ttaggatttg ttctgtgatt
      841 ccttgaatct tgatatccat ctgtaaagca gtgataccat cttttgtacc ggcaacttta
      901 aagtccatat cgccaagatg atcttccagc ccttggatat cagttaggat cgtatagttt
      961 tcaccatcag aaacaagtcc catcgcaata ccggcgactg gcgctttgat tggtacacct
     1021 gcatccatca atgctaatgt gccagcacag atacttgctt gggaagaaga accatttgat
     1081 tccaatactt ctgctactaa acggatcgta taaggaaatt cttcttctga agggattact
     1141 tgtgctaacg cacgttcacc taatgcgcca tgtccgattt cccgacgccc tggagagcct
     1201 gcacgtcctg tagaaccaac agaaaattga gggaaattgt agtggtggat gaatcgtttg
     1261 ctgacttcca cccctaaacc atcaatgatt tggtgctctc ctagaggtgc gagtgtacaa
     1321 gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg atccatgtac acgtggcaat
     1381 aagctgacct ctgatgacaa tggacggatt tcatccaatt tacgcccgtc aggacggatc
     1441 ttatcgatgg tgatcaattc acgtacgaca tctttttcaa gatcttctgt gatttgttta
     1501 acttctttca acaagtgatc ttcatcttca tgacctgcaa atttctcagc ataagcttcg
     1561 cggatatcga tcttcacctg ctcgatatta tcttcgcgag caagtttttc ttcagtcatt
     1621 actgctgctt tcattgtagc gtaagaagca tcaaagattt cttttttcaa tacttcatca
     1681 acttgcagta aagtcacttc cattttttct ttccctactg cttgaacgat ttcttcttgg
     1741 aaagcaacta actctttgat agcgtcaaaa ccaaacagca atgcaccaag catgtcttct
     1801 tctgaaactt ctttcgcgcc gctttcaacc atattgatgg cttgtttcgt tccggcaaca
     1861 gtcaattcga tatctgtttg ttctgcttgt tctactgttg gattcaagac gtactcgcca
     1921 tttacacgac caacttctac gcctgcgatt gggccgtcaa atgggatatc tgagattgat
     1981 agtgctaaag aagaacctag cattgctgcc atagctggcg agcagtctgt ttctacactc
     2041 atcacgatgt tcgttacttg gacttcatta cggaagcctt ctgcaaacat cgggcgaatt
     2101 ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac ttggacggcc ttctcgtttg
     2161 atgaaacctc cagggatttt ccctgctgca tacatttttt cttcataatt gatcgttaat
     2221 gggaaaaagt ctgtatcttt tgcttcttta gaggccacag ctgcacttaa gactactgta
     2281 tctccataac ggactaaaac ggctccgttt gcttgttttg ctagctgacc gacttcgatt
     2341 tgtaagggac ggccgcccca tgtcgttttg aatacttgtt tttctgacat aacttctcct
     2401 tttccttctc gtgaaagcaa gacgctacta aacaaatgaa aactgccgaa agccgtcttt
     2461 tgagcagctt tcatttggtt agtaggagtc catctagctt gatcacggaa ttttacttaa
     2521 ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc actctttttg actaaaatta
     2581 acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt tgaacgtcag ttttacgtaa
     2641 gtaagctaac aagttacggc ggtgaccaac ttttttcatt aggccgcggt atgaatgatg
     2701 gtcttttttg tgcacgcgtg catgttcatt caagtggttg atttcttcag ttaatacagc
     2761 gatttgtact tctggagaac cagtatcccc ttcatggcga gcgtattcgt tgatgatttc
     2821 attcttgcgt tcttttgaaa ttgccatttc tttcacctct ttcgattatt cccctgcgac
     2881 tgagtaaaac gttggtgatt cgcttaacca agtggcaggt catgtgccta gagcacacag
     2941 ctatacttta cctgaaaccg ggttgaaaat caagtagtta atcattaaga aaaggtagtt
     3001 ttcaaacaat cttactacta cttttccatt tttgtggtaa actataatgc gaaaatagac
     3061 ttgaaacaca gtcttttaag aaaggggtac atattccatg attacaatgg atgatatcat
     3121 tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa gtttctcttc cattaagcga
     3181 agaagatatc tctttaggaa aagagatgct tgagttctta aaaaacagcc aagatccaat
     3241 caaagctgaa gaactacact tacgaggcgg tgttggctta gctgctcctc aattagatat
     3301 ttctaaaaga atcattgctg ttcacgtacc aagtcctgac ccagaggcag atggaccttc
     3361 tctaagtact gttatgtata atcctaaaat tttgagccat tctgttcaag atgcttgttt
     3421 aggggaaggt gaaggctgtt tatctgttga tcgagaagta ccaggatatg ttgttcgcca
     3481 tgccaaaatc actgtgtctt attatgatat gaatggtgaa aaacacaaga ttcgtttgaa
     3541 gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat atcaacggtg tgatgttcta
     3601 tgaccacatc aacgatcaaa atccatttgc actaaaagaa ggcgtattgg tgatcgagta
     3661 gagaattggc aggttccttc gatactatgc tacaagaaag ctttataaaa aaggggagtg
     3721 ctcaaaagct gcacttccct tttttatgtt tctccactaa tcgaaattta gatagacatc
     3781 tagaaatcgc aattccaaaa aattctgtgt attggatacg catcttttct tattttttct
     3841 tatttcttcc atatgggtgt ttatctcaga gatgatagaa tagtttctag gataaaaagt
     3901 agcagctacg atgttatcct tcgtagagga ttcctgcttc agctagtttt tgtttgatgt
     3961 ttttcagcat atgcttttct gggtagcgga tcgtatgcag atggatgccg cctgttaatt
     4021 cggataacaa agtagcatta cttttttgat aacgtctcat aaaggaatcg atatcttgtg
     4081 gcgttttgat gtgcaaagtt cctgctaact caccgtaaag gggatgttcc acaattacat
     4141 caatgatttc accaccattt tcaacgatta gtctcagttc ttcttctgtt tgatcttttc
     4201 catgctggac agcgatttta ctgatgtgcc cagtattggt ttcttctgtt tcgaggatat
     4261 aacctcttgc tgttgccacg atacttttac ctgcagctcg tagtaaagca atatcgccta
     4321 caatgatctg acggctgact ccaaaagttt tcgcaaactt gctagcactc actggttttt
     4381 ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt ctctccttcg atcacactta
     4441 ttcctccttc attttgtttt ggtattcgcg cgagtgtttc atcaaatcaa catacgtgtc
     4501 tgttattgtt tccttatatc ccttattttt cagcagacta acaactttat ccaatacttg
     4561 ctgctctctt tttgcatcat aaactgcttg gttttcttct tttttcactt gtcctatttt
     4621 agttaccgta tccattcttt tttctaataa agcaaccaac tctcgatcga tcatatcgat
     4681 cttatgacgt aatgtttcaa gagactcttc tgttagttct tcctctactg tcttgatgat
     4741 acgagcagga tttcctgcaa tcactacatt gtcgggaaaa gatttggtca caaccgaacc
     4801 tgcgccgact actacgttgt cccctagagt gacacccggc gtgacgataa ctcctccgcc
     4861 taaccaaaca ttattaccga ttttaatggg ttttgcgtac tccagaccgc tgttacgttt
     4921 cactggatgt agtggatgcg tcgccgtgta aagctggaca ttcggagcga acatgcagtt
     4981 atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa ttgaaatttg cataaaaatt
     5041 ctccccaaca aatatattat atccgtaatc aaaattgata actggctcca tatagatttt
     5101 tttccctgta cggccaaaag tctctttcaa caattgggat cgcagttccg ctgattctgc
     5161 ttggttgatg atttggcttt gacttcttgc aaacatacgg tcggcaacca gctcaggatc
     5221 attggcaaaa taaagctcgc ctgcaatcat tttttctttt tcggttttga attcagtcat
     5281 aatctgctcc taattcttgt atttttttga aaacaaccgc tccgtcttct tgaataaacg
     5341 tataaggctc cgctaaataa acaggcgttt gattgattac taagattttg gctgacggct
     5401 ggcgaaactg taccagatca caaaacggat gaacttgaaa actcgttcca acaatgacga
     5461 tcagctctgc ttggctgacg gcataagccg cctgctccat gactccatct tgaaaccctt
     5521 caccatataa cacgatgtct gggcgtattt gtcctccgca tgcttgatgt ttgtcgctgt
     5581 gaagatattc tttccagtct accgtttgtt gacattttcg acaatgacat tgatacagac
     5641 tcccatgaaa attgacgagt tgcgggcttt tagcctttgc gtgcaaaccg tcgatgtttt
     5701 gggaaacaac ccaaacattt ttttctttcg ctagatgtgc tatttccaaa tgaatgatat
     5761 tgggttttgc ttcaggatga taaatctttt tgacaaattg atagaattta tccggttcat
     5821 tcaccattgc ttgatgactc agcaagtatt ccggctgctc catcccgtgg tagattcctg
     5881 acaaagatcg atagtctggc actccagaag gtgtggatac ccctgcaccg gtcaaaaatg
     5941 taatttgctg ataagctaat atctcttttg ctgcttcgtc tactgttaat tgtttcatgt
     6001 tttgtcacct cactttccat tatagcccat actcgaacaa atttattgtt gaaaagtcag
     6061 aaaaatggga ataaaaaagt gttatgggca tttgagcaat ttatcacaat gttcactgac
     6121 aaaagaatga aaacgtgtta caatactgct gtataaaagt gaggtgaatc gatatgttac
     6181 cattctttat ttattggggc gttattctgg cttgtatcgc ctggttagcg ttaagcgttt
     6241 atttctctgt tttttatctt gtacgcaaag aaaatggaaa tctttgggct tttgctttat
     6301 tcaacgtgat tgccgcaatt gttttagcca ttacattagc cgtatatcga acatggggct
     6361 ggggaatcac ccaatactca agtttgattt atctaatatt agccatttat ggcgtcgttg
     6421 ttcttttgca agcgatccta ggacgtgagc caaagaaagc ggcagcttaa atgatcgaga
     6481 tggaactgga cacagg
//
LOCUS       DACIRY010000137         6466 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.143, whole genome shotgun sequence.
ACCESSION   DACIRY010000137 DACIRY010000000
VERSION     DACIRY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.143"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            14..751
                     /locus_tag="IYE22_002383"
     CDS             14..751
                     /locus_tag="IYE22_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507569.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            765..1580
                     /locus_tag="IYE22_002384"
     CDS             765..1580
                     /locus_tag="IYE22_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ9507570.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1593..2240
                     /locus_tag="IYE22_002385"
     CDS             1593..2240
                     /locus_tag="IYE22_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ9507571.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2256..2912
                     /locus_tag="IYE22_002386"
     CDS             2256..2912
                     /locus_tag="IYE22_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ9507572.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3074..3895
                     /gene="racE"
                     /locus_tag="IYE22_002387"
     CDS             3074..3895
                     /gene="racE"
                     /locus_tag="IYE22_002387"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ9507573.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3898..5253
                     /gene="rph"
                     /locus_tag="IYE22_002388"
     CDS             3898..5253
                     /gene="rph"
                     /locus_tag="IYE22_002388"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ9507574.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5254..5772
                     /locus_tag="IYE22_002389"
     CDS             5254..5772
                     /locus_tag="IYE22_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ9507575.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5879..6379
                     /locus_tag="IYE22_002390"
     CDS             5879..6379
                     /locus_tag="IYE22_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ9507576.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2119 a         1089 c         1434 g         1824 t
ORIGIN      
        1 ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg gataaatatt atggcaagtt
       61 ccacgcatta aaaaatatca atctcgaatt cgagaaagga gaagttgtcg ttgtcattgg
      121 tccttctggt tcaggaaaga gcacgatgct tcgctgtatc aatggattag agacgatctc
      181 ttcaggtaag ctgctgatta atgatactga cttgcatgat aaaaagacaa aactgacaga
      241 agtccgaaaa aacatcggca tggttttcca acatttcaat ttgtacccaa ataaaactgt
      301 tctggaaaac atcaaattag caccaatcaa agtgttgaag caagaccagg caactgcagt
      361 caaaaacgcg gagaaatttt tgaaaactgt caatatgtta gataagaaag attcttatcc
      421 ttccatgtta tccgggggac aacagcaacg ggtggcaatc gccagaggtt tagcgatgaa
      481 tcctgagatg ctgctgtttg atgaacctac ttctgctttg gatccggaaa tgattggtga
      541 tgtattagac gttatgaaaa aattggctca tgacggtatg tcaatgattg tcgtgacaca
      601 tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc ttcatggctg atggagaagt
      661 attggaagac agcagaaatg tgagagactt tttcgaaaat ccaaatgaag tccgagcaca
      721 gcaatttatc agtaaagtta tcaatcattg ataggaggaa aaagatgcgc aggttaaaaa
      781 aaatacgcct gattttcctt ttaggcattg cttttcttgc actagccgga tgtaaaggaa
      841 atgggttggc aaatgaagat attttagagc gaagcaaatc taccaatgaa atcatctggg
      901 gtgtcaagta tgatactcga ttatttggaa tgatggatat tgagagcaga actgtacaag
      961 gatttgatgt agatattgca aaagcaatca ctaaaaagat ccttggtgac aatggaaaaa
     1021 cagaatttgt tgaagtcact tctaaaactc ggattccatt attgaaaaac ggaaatatcg
     1081 atgcaattat tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt gacttttcag
     1141 atgtctattt cgatgctggc caagctttgt tggtaaagaa aggaagccaa atcaaaagtg
     1201 tagatgattt gaatgcttct acaacggttc tagcagtcaa aggttctaca tcggctgcga
     1261 atatccgaca gcatgcacca gatgcaaaaa ttttagaatt agaaaactat gcagaagcat
     1321 ttactgcttt acaatctggt cagggagacg cgatgactac ggataatgcg attttgttag
     1381 ggattgcaga tgaaaatcca gagtatgagt tagttggtgg aacgtttact aatgagcctt
     1441 acgggattgc gatcaacaaa gggcaagaaa acttcttgaa agctgttaac caagcgctag
     1501 aggaaatgca tgcagatggc acatacgata aaatctatca aaaatggttt ccaaatgaaa
     1561 ctgaaggaaa agtagagtaa gggggagagg caatgagcga attgatacaa acttatggac
     1621 caatctttgt cgacgggttc aagatcacat tgttttcgag tgtcttagca cttgtgttta
     1681 gtttgattat cggaacgtta atggcaatct tccaattatc aaagaataaa gtagtgagcg
     1741 ccttagcaaa ggcatatgta gaatttttta gaaacattcc attattaatc atcgtgatgt
     1801 ttttttatgt tgtcgtaccg atttattggt ttaatctaga tggattccaa gcaggtacga
     1861 ttggattaac gatctataca tctgctttta ttgctgaaac agtacgttca gggattcaaa
     1921 ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg ttttacctat tcagaaatca
     1981 tgcgttatat tgttttaccg caagcattca aaatcgttat cccgccatta ggcaaccaat
     2041 ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt agctggttta gatttgatgt
     2101 atcaaggaga cttgatcgct agtgaaacat tcaatacctt cgatacgtat atcatcgttg
     2161 ggttatttta cttagttatc acgctaccgt tatcttactt aatggtttat cttgagaaaa
     2221 aatgggcggt tcgttcatag aaggagggga aatacatgga ttttagcggc gctttttcat
     2281 ggatgaatat ccgtttttta ttggaaggat taaaggttac tgttgaagtt tcattgtttt
     2341 cgatcatttt tagtttcttg atcggtggtc tgacaggtgt gattcgtttt gcgaatatcc
     2401 ctttcttttc caaaatcctt ggtgtagtga ttgatattat acggaatctt ccattattat
     2461 tgatcatttt cttcacttac tttgccttac ctcaaatcgg cattcagatg aacatcttct
     2521 ggtccgctgt cgcagcatta acgatttttg agtcagctat gttgtcggaa attttccgag
     2581 ctggactcaa tgctgttcca aaaggacaga tggaagcagg attatcaacg ggactgactt
     2641 atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg atcccggcaa
     2701 ttgtgagcca attgatttca ctgatcaaag atacttcatt agcagtaatc atttctttac
     2761 ctgaactttc tcatcaggca cgtattattt atggacagaa caccaattat gtgcttccga
     2821 tgttcatcat catgacactt atgtacttcg tggtatgtta cgcgctttct ctgctttccg
     2881 gttacttgga aaagagacgg tacagttatt aatagatgat tcttttagcg gttgtggcaa
     2941 actaaagtca caaccgcttt tcctctttcc attcgaaaaa aagagatttc gattgtttta
     3001 agcaagtaag agcacttgaa aattcacgct aaaaagtaca caatagggag gatagaattt
     3061 attgaaaaaa aggatgatac gattgacaga taatcgccct atcggattta ttgattcagg
     3121 tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg aaaatatttt
     3181 atttgtagga gacacagcac gctgcccata tggccctaga cccgcggaac aggtaatcca
     3241 gtatacttgg gaaatgacgg attatctggt ggagcaagga atcaagatgc tggtgatcgc
     3301 ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa gctgctcttt ctattccagt
     3361 catcggtgtg atccttcccg gtactagagc ggcagtaaaa aaaacacaaa ataaacaagt
     3421 tggcattatc ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag cactgaaaga
     3481 gaaagtacca gaattgactg tgacaagtct tgcttgtccc aaatttgttt cagttgtcga
     3541 aagtaatgaa taccattcat cggtggcgaa aaaaattgtg gcagaaacat tagctccttt
     3601 aaccactaaa aaaatcgata cattgatttt gggatgcacc cattatccat tattacgccc
     3661 catcattcaa aatgtaatgg gagaaaatgt tcaactgatc gattctggag cagaaacagt
     3721 aggtgaagta tctatgctgt tagattattt caatttgagc aattcaccgc aaaatggtcg
     3781 gacattatgc cagttttata caactggctc tgccaaactt ttcgaggaaa tagctgaaga
     3841 ctggcttgga atcggacact taaatgtaga acatatcgaa ttgggaggaa aataaggatg
     3901 cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac aaatgttttc
     3961 aaatatccag aaggatcggt agtgatccgt tttggtgata caacagttat ttgttctgca
     4021 acaatagaaa attctgttcc cccttttttg agagaaacag gaacaggatg ggtgacagca
     4081 gaatatagta tgctgcctcg agcaacgagt acgagaaatc gtagagaaag cagcaaaggg
     4141 aaactttctg gtagaacaat ggaaatccag cgtcttattg gtcgttcctt gcgtgcagta
     4201 gtagacttgg aaaaattagg agaacgtagt atcatcgtag actgcgatgt gatccaagcg
     4261 gatggcggta ctagaacagc aagtatcact ggggcatttg tggcattgaa attagccatt
     4321 gaaaaacttt taagagaaaa agaattgtca gaagatccaa taaaagaaca tcttgctgca
     4381 gttagcgtag gaatcttacc agatgggact tgcgtcacag atttggatta ccaagaagat
     4441 tccgcagcac ttgtagacat gaatcttgta atgacagaat ctggaaaatt cgtggagatt
     4501 caaggaactg gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat gctctttttt
     4561 ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac atgctttgct aacagagttt
     4621 gctcaaaatg atgagcggat agaagaaaca aaaacaatca tcattgcgac aagaaatcca
     4681 ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg gatatcatgt aaaaacatta
     4741 cttgactatc ctgaattgcc agatgtggaa gaaactggca gcacatttga agaaaatgcc
     4801 cgattaaaag cagaaacgat tgcacaattg cttgatcagc cagtcttagc agatgattca
     4861 ggtttgaaag tggatgcttt aggcggtatg ccaggaatct attctgcacg ttttgcaggg
     4921 gaacaaaaaa gtgatgcggg gaataacgcc aaattattat atgaattgac agatgtgccg
     4981 gatgaaaaaa gaacagcaca attccattgt acgttagtat tcgcggcacc caaaaaagac
     5041 agccttgtag tagaagcgga atggcccggt cgagtagccc gtataccatc tggcgaaaat
     5101 ggctttgggt atgatccttt atttatccca gaaggtaaga aacaaacagc agcagaatta
     5161 tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa attgagtgcc
     5221 gagtggaaac aatggctgga aggagagaga tagatgcggt atttagtagt aagtgataat
     5281 catggagaca gagatatcct cgttgactta gtagatcgtt atcgttctga agtggatatc
     5341 tttttccatt gtggtgattc ggaattagaa gcttctgacc cattatggga tacattccaa
     5401 gtggttcaag gtaattgtga ttatggacca ggattcgaac agaaaaaagt gatccaaact
     5461 ggtcaggata cagtattcat gactcacggt catttatcga atgtccgatt tggtttgaca
     5521 cagttagcaa tcgaagctcg agctgcaact gccgatatgg tgttttttgg tcatacccat
     5581 caaataggct gtgagatgga tcagaaaatc ctgtttttga atcctggtag catttctcag
     5641 cctagaggcc cgatccaaat cccatcatat gctcttatcg aaagtttaga tgaccaaatt
     5701 tctgttcaat actataaccg tgcccatcag gcagttgaag agttggcctt ttctttttcg
     5761 agagagcgct gaataaaaaa actctaattt ttaataaaaa taagctttat tttatgaatt
     5821 ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat agaaatggag gccatcagat
     5881 gattggacct atagtaagag aaatgctgct ggaaaatcag gaaacgtttt tgattcctgc
     5941 agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc catggtttac ttgtgttatc
     6001 gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat gatcactttg taggactagt
     6061 aagtttatca gatgtggtca ataaaatgat tgacttgcaa acaatcagca tggacccgtt
     6121 agaaggacta acggttgctg atgtgacaga aacagatgtt ccgacaattg atgaaaattg
     6181 ggaactagaa gaagtactcc atttgttagt cgattcgcct tttttaccgg ttgtcacaga
     6241 agatcggatt ttcaaaggaa tcattacaag aaaagagctg cttaaagccg taaacttcat
     6301 ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat taaaagaaca
     6361 aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat aagtagaaaa
     6421 aacagcgtac cagttttgac tttatcttga aaaagaggct gggaca
//
LOCUS       DACIRY010000138         6455 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.144, whole genome shotgun sequence.
ACCESSION   DACIRY010000138 DACIRY010000000
VERSION     DACIRY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.144"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..840)
                     /locus_tag="IYE22_002391"
     CDS             complement(<1..840)
                     /locus_tag="IYE22_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ9507577.1"
                     /translation="MSERISYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLT"
     gene            complement(1018..1821)
                     /locus_tag="IYE22_002392"
     CDS             complement(1018..1821)
                     /locus_tag="IYE22_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ9507578.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1967..2881
                     /locus_tag="IYE22_002393"
     CDS             1967..2881
                     /locus_tag="IYE22_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ9507579.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            2929..3399
                     /locus_tag="IYE22_002394"
     CDS             2929..3399
                     /locus_tag="IYE22_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ9507580.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3484..4092
                     /locus_tag="IYE22_002395"
     CDS             3484..4092
                     /locus_tag="IYE22_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ9507581.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4226..4426
                     /locus_tag="IYE22_002396"
     CDS             4226..4426
                     /locus_tag="IYE22_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9507582.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            5180..6406
                     /locus_tag="IYE22_002397"
     CDS             5180..6406
                     /locus_tag="IYE22_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ9507583.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2187 a          984 c         1293 g         1991 t
ORIGIN      
        1 tgtcaaacct gcaatgtggg aagtaatcag tacattgtcc attttccaaa gaggatgatc
       61 ttgtggaaga ggctcttcct caaaaacgtc taatgctgca aaggaaagtt gctggttatt
      121 taaagcctgc accagatctt gtgtcttaac agaagcacca cggccaacat tgatgaatac
      181 agattttgga tcaaagtgtt caaacatatg ctgatcaaag agcccagttg tttcctccgt
      241 tagaggtaag atattgacta cgaaatctac ttcaggaaga acttccttta gttgatttaa
      301 agaaaaagtc ttttccattc cttctgctgg atgaccggat gtattgattc catagcagtt
      361 catgtctaat gctttgcatt tttttgcaat catttttcca atatggccag ttccaatgat
      421 tagtattttt ttcccttgga tagattggta atggaccgat gaacctaacc atttcatgtc
      481 actctgtgcc ttttgtgctt gaaaaagacc acgagaatac ccaaggatga ttcctaagac
      541 atgctccgta atcgattcac tgtggatgcc actaccattt gaaagtaaaa tatgtttctt
      601 tgaaaaagtc tcaaggggta gctgatcaac accagctgag atagattgga cccatttcaa
      661 ggtagaagag gctaatagtt tttcttggta gtctgagttc cagcccaaac tgatctctac
      721 attttgtaga tcctctggtt gtaattctgt tttaaagata taaccaggcg ctatagtatg
      781 gatttcttcg ataaattcat cacgaaatgg acgatctgca taactgattc tttctgacat
      841 agttaatttc ccgcctttct ttactctaag tgtagcgcat acaagaagaa ctttgaagcg
      901 ccttgcttgt tttttgagaa agaaatattt ttcaaataaa aaaggggctg tgacaaaaaa
      961 atgtcacaac ctctaaaagg gtctattgaa gtagaaataa cgagttgtct ttatttctta
     1021 attgatcgat atttttgccc atcctttgac agcttgagca aaaataaaga ttcctgcaaa
     1081 agcaatgaag gcgctagtgt caaaagaagg aagaaagacc atcaagattg ttgcaattat
     1141 agtaggcaat gaaattgaat agatcaatgt ttttaaacaa tcaagaaaag tagcttttgt
     1201 tagtcgaaag cgtgcataga gaaaagcagc aatgttggta aatagcaaag tgatcagcag
     1261 attcagaaaa ctaggataaa tagaaaatat aaatgtcagg acttttatcc aaagtggtat
     1321 agatgcttca cttaaaaaag aacggagctg cttacctgtt aagtttttta aagattcatt
     1381 ggtgtaggga agttctaatt ggttattgtt caacaaagca gaagtcgtgc ctgtattagg
     1441 aagagctatg ataagtttgt tttttaacat cccgacacta acaaaattcc ccatcaaatc
     1501 agtagagatg tcctgttctg ttcgttttcc ctctggatca aatgtgaaga taatggagtt
     1561 tgtttggtaa atgaatcctt ctttttcttc ggtttgaagc tgcccgtttt ggattgtaaa
     1621 atcaggaatg cgagtagcaa tcttttgccc atccaattga atgttgttta agacttgaaa
     1681 aacttgaaaa gagattggca aagccataat tatactcaaa actaaaagat agacaatggc
     1741 tttccaaaat gggacatttc tcgcctcctt taggtctttg aatcgtataa aggaactgat
     1801 aattaattgt tttgttgtca taagtactcc tcatctagta gtgtcatcac ttgcaattct
     1861 agcgaagtat caagtgaaat acaagtgctt taaggaaaca gctgaatgat tggtgaaata
     1921 tcaaaaaaat gttattcttg tctaaagact atccaatata aataaaatgg aggaaggaat
     1981 agtgcctaca cagccacaga gatcatcaaa aaaactattt ggagtagtgg ttttgaatgc
     2041 ggtgatcact ttatcagaat ttattggagg gattatgtca gggagtttgt ctcttgtttc
     2101 agatgctttc cataacctta gtgatacatt ggcaattatt ttttcttacg gggcacaaaa
     2161 aatggcccga aaagaagcaa ataaaaaacg tacgtatggc taccagcgtt tagaaatatt
     2221 gagtgctttc atcaactcat tcatattgat catattatcc ttgtttttaa ccgttgaagc
     2281 attcaagcgg ttcacttcac cagaaaagat aaatagtcat ttgatgctaa cagtagcagt
     2341 tattggattg ctcgcgaatt tgttctcagt attattatta cgacaagaag cagatgaaag
     2401 tttgaatata aaatctagtt atttgcattt gttaagtgat acactgtctt cggtaagtgt
     2461 gattatcgga gcaattttga tccgtttttt cgatatctac tggatcgatc cagttattac
     2521 actgatcatt tcgatatata tattgataga agcgataatg gtgatcaaaa aagctgccgc
     2581 tattttgata caaagtgcac ctgctatcga ctatgaaaaa atggaacaag aaattgaagc
     2641 aatcgatggg gtaaaggatg ttcatcatgt ccatatttgg cagtatagcg aaaagataat
     2701 tattttcgac ggacatattg attttgaaga tcagctacta agcgagattg aaaagatata
     2761 tcctcgtata atttctttat taaaattaaa atatggtatt acacatgtga cgatccaagc
     2821 agagacaaag gcaaaggatc aaaaaaaatt aattttttta aataaagatg aaagttattg
     2881 aaatatagag tatgataaat aagaattttt aataaggtag gttggctcat gaagcagtat
     2941 cgacagttaa aaggatattt agaagcaaaa ggatattggg agatactagt ctatctcttt
     3001 tttggtggtt tggctacgat tgtaaatttt gttagttttg ctctcgcacg ccaatatttt
     3061 gatttaagta tggccttatc caattctatt tcgtggtttt gttctgtatt gtttgctttt
     3121 gtaacgaaca aactttgggt attccattca aaatcaccga attttacaca tgcacttatt
     3181 gaatgcggta agttcttctt ctaccgtatc ctttcttacg gattggacat gggggcaatg
     3241 gtattgttga tcaatgttat gaacagcaat gagtatgtgg cgaaaatcat cactcaaatt
     3301 attgttatcc ttgcgaacta tattttcagc aagttgttta tttttaaaga aactgaggtt
     3361 tttgaagaag aagtaggtca aagtgaaaaa gacgactagg cataacaatt gaagaatttc
     3421 cttctctttg ttactctttg taatgtagac attattaaga aaacattgag gaggaatttt
     3481 tttatgactt acacattacc agatctacct tatgcatatg acgcattgga accttatatt
     3541 gacgaagaaa cgatgcatct gcatcatgat aagcatcaca atacttatgt gacgaattta
     3601 aattcagcaa ttgagaaata cccagaatta ggcgaaaaaa caatagaaga attattatct
     3661 gatatggacg ctattccaac agatatcaag acagctgtac gtaacaatgg tggcggacat
     3721 gctaaccatt catttttctg ggaaatcatg gcaccaaatg ctggtggcga acctacagga
     3781 gaaataaaag aagcgattaa tgaagctttt ggtgattttt cttcttttaa agaagaattc
     3841 aaaaaagcag ccgctggacg atttggttct ggatgggctt ggcttgtaat ggaaaatgga
     3901 aaattagcta ttacctctac tgcaaatcaa gattctccat tgatggaagg aaagacacca
     3961 attctaggtt tggacgtttg ggaacatgct tattatttga aatataaaaa tgtccgacca
     4021 gattatattg cagcattctg gaatgttatt aattgggatg aagtaaacaa acgtttcgaa
     4081 gcagcaaaat aaccatttcc tccgtcatat cagtcttaaa aataatttgg aaaataaaag
     4141 agcgaaagct tgctagcgtt ttctaaattg ggtataatgt tcttgcaagg ttacttgcaa
     4201 gaactttttt ggaggatata catttatgga acacggaacg gttaaatggt tcaacaatga
     4261 aaaaggattt gggtttatta cagtagacgg cggagatgac gtatttgttc atttctcagc
     4321 aattcaagga gacggcttca agagcctaga agaaggtcaa gaagtagaat tcacgattgt
     4381 tgaaggtgca cgcggacctc aagctgctga agtgactaaa ttataataat ttgttgaata
     4441 aagacacgac ttcttagaga gtcgtgtctt tttgtctatt tatgaagttt ttagattttt
     4501 tggctaagta aatgcatttg ttcacttgac atgtcctaac taattatcaa gcaaggaaca
     4561 ttttcttcgt tagtaatgga ttttaaagaa atttcagttt ttggatgttg aataaaagac
     4621 catttgtgaa accactagta tacatactca ttaccatgtc aattctatta ctttaaaaga
     4681 aaatcgtttt actttgaaga ggagcatatc tatccattaa aagatgatgg taatgcaatc
     4741 actagttcga gtcattttaa gagccatgca gtgagctgga aataaaacga atagaagatg
     4801 ggtttaacaa aaggaccagt cataggaaga aattgtcgtg aaatcgaatg ataatctgaa
     4861 cggtaggcaa agactgttaa aaagatggga aaggtatgtg cgatcatttt actgcacgat
     4921 tatttggaaa ataccttgtt gttcgctttt tctggaaaaa atacataaaa aaagataaaa
     4981 cgattgcgaa ctgcgtgtat tcggtctttc gatctttttc acggtatggt atgatagtta
     5041 agaattttga gaaattttaa aaaataataa tcgttacaca aaaatgtttt ttaaagtttt
     5101 cttatgatta aaaagtgaat ttgctgattg aacagttata tgagacaata ttttagaaaa
     5161 tgtgaaacag aggtgtatta tgaagcgatg gcaaaaagtt ctattaacct tgttaggggt
     5221 attcgttttg ctgattgccg gattatcagc atacggaatc agatttatgg gagaagcgag
     5281 ccaaacagta aacaaaattt ctaaaaactc taatcgaata tcctccaaac gttctgagag
     5341 agtaagcatt gatgacaaag aacctttttc ggttttgcta ttaggtttag ataccggtgg
     5401 attaggaaga acggaacaag gacgttctga tacgatgatg gtagtaacag ttaatccgaa
     5461 acaaaaaaaa tcaacgatca tcagtttgga ccgagatatt tatacgaata tcgtaggtta
     5521 tggaacggta gataaattaa atcatgccta cgcatttggt ggtgtcgaaa tggccatgga
     5581 ttcaattgag caactgctcg atattcctat tgatcattat gtaacgatca atttggatgg
     5641 catggaagat ttgatcaatg cggtcggtgg catcaaagtg aataataaaa ttgactttac
     5701 tttggatggt gtccatgtac cagtaggtga acaaacattg aatggagaaa aaggtttagc
     5761 ctattctcga atgcgacacg aagatccaga aggcgatatc ggtcgacaac gtcgtcagcg
     5821 ggaagtagtg acgaaaatcg tcaataaagt tttaagttta gatggcgtaa gcaattatag
     5881 aaaaatttta aaagcagttg agaacaatgt gacgacggac ttagattggg atgacatgct
     5941 tgatgtagcg acgaactaca ccccggcgtt tgaaacaatc aaacaagacc agttgcaagg
     6001 cgaaggacaa atgatcaatg atatctacta tcaaatttta ggaaaaaatg atcttctttc
     6061 gatccaaaat gaactgaaga aacaattaaa tataaaaaca agcgatacat tacctaattt
     6121 gaaaaatgag aatgcttcaa tgatgttctt tgatgattcg gaagatggcg attcgacaaa
     6181 cggtgatgga tcgaatagtg aagggtacac cgattattct tcctattcag aaaacacata
     6241 tgatcagtac caagatccga atacctatta tcagcaaaca gaagatacaa gtcagtacta
     6301 tacaccaagt caagatactc aaagttatga tccttacgct ggacaggaac aaaatggtta
     6361 tggttatgac cagaataatg agtatggaac aacaagtggg tactagaaaa aaatagtttg
     6421 gttaataaag agtgccacct aaattagaca aaaaa
//
LOCUS       DACIRY010000139         6344 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.145, whole genome shotgun sequence.
ACCESSION   DACIRY010000139 DACIRY010000000
VERSION     DACIRY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.145"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1076)
                     /locus_tag="IYE22_002398"
     CDS             complement(join(<1..800,800..1076))
                     /locus_tag="IYE22_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9507584.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYE"
     gene            complement(1117..1371)
                     /locus_tag="IYE22_002399"
     CDS             complement(1117..1371)
                     /locus_tag="IYE22_002399"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9507585.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(1482..1736)
                     /locus_tag="IYE22_002400"
     CDS             complement(1482..1736)
                     /locus_tag="IYE22_002400"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9507586.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(1929..2204)
                     /locus_tag="IYE22_002401"
     CDS             complement(1929..2204)
                     /locus_tag="IYE22_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ9507587.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(2176..3129)
                     /locus_tag="IYE22_002402"
     CDS             complement(2176..3129)
                     /locus_tag="IYE22_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ9507588.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            3741..5234
                     /locus_tag="IYE22_002403"
     CDS             3741..5234
                     /locus_tag="IYE22_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ9507589.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            5369..5665
                     /locus_tag="IYE22_002404"
     CDS             5369..5665
                     /locus_tag="IYE22_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ9507590.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(5689..>6344)
                     /locus_tag="IYE22_002405"
     CDS             complement(5689..>6344)
                     /locus_tag="IYE22_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ9507591.1"
                     /translation="CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSI
                     HEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENK
                     LNHWFESVPKNLFKSITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGL
                     LRRDGLLKSMDFNSVDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1993 a         1098 c         1093 g         2160 t
ORIGIN      
        1 ctcgtagcca agtgtaccgt gcaaccgaag gtggttccac caattgacat agtcaaataa
       61 ctccaaatcc aattgttgta aggtttcaaa tgtgtattga tagacaaatt ctactttcaa
      121 cgacttataa gttgattcag ctacggcatt atcaaaagga cagcctttat gactcaatga
      181 tcgattgatg tcaaaagttg ttaataattc atcaatagct tggttatcaa actcttttcc
      241 acgatcagta tgaaaaatct caacctctgt cagaggttgt ttgatacggc taaatgcttt
      301 ttttactaga acggcatctt tatgttctcc acaagaatag ccgagaattt ctcgattgaa
      361 cagatccaaa atgaaacaga cataattcca ttttttcccg actcgtacat aagtcaagtc
      421 tgttacgatc gcttctaatg ggttgtctct taagaattta cgattcaata cgtttgtcgt
      481 tttggcttca ttgcaagtag aatgatgtac tttaaaataa gcaacagtat agctcgattt
      541 taatcctcta tttttcatga ttctactaat ttttcgtcgg ctgatctgaa tgcctcgttt
      601 tgataaggct ttttttattt ttcttgagcc gtaggccttt cggctgcgga taaattcttc
      661 agcgactact tcttcaagtt ctgattcgtc tttctttggt tttgattgat aataatagga
      721 ctgacgtgat agacctaata ttctgcacat cgctgatata gggtaaagat gcttattcgc
      781 atcgattact tgtctcttcg tccgaatatc agcgctgctt gctttaaaat atcattttcc
      841 atttctaatt gctggtttct tttacgtagt tctaacaatt ctttttgttc aggcgtaaga
      901 ttatcttttt ctttgaatga accactcgtt ttaaattgct ttacccattt gtcaaatgct
      961 gaagccgtta gttcatattc tcgaatgatt tctacacgtg gctttccagc taagtaaaga
     1021 ttgacgattt gttgcttgaa ttcttgtgaa aaagttcttc gtgttctctt agacataaaa
     1081 attcctcctg gtatgttttc ttctagtcta cacaccttaa tttttctgtc tagttaattg
     1141 tagcctatcc atgatcggtt tattagacct ttgaggtcta tattttttaa ctacaatcga
     1201 ccttaaattt aattgtttca ttagcttttg gacatgttta agagatactg aaatcccttc
     1261 ttttaataaa acttgatgga tctttgtggc accatagcgt tgtttattcg taaaaaaaat
     1321 tgttgaaatc ttctttttca acttattatt tctgatttct gtagctgtca acggtttatt
     1381 cttataaaaa taatagacac ttctagggat ttctaataac cgacacaaca aagaaacagt
     1441 gtaatcctta caccattttt caataaattg aacaagcgaa tttaggtttt ccgagagaat
     1501 atggctgcgg ctttttttaa gatttcaagt tcctcattta aacgagcatt ttcttttctc
     1561 agttcagcta cttcttttcc agtcagtcct gtggactgat caggtaaata taaattcttc
     1621 catttgtaaa tcgttgcttc agataagcca tattctttcg ctaaccctcg aacagaacgt
     1681 ccagtttgat tcagttcaac aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa
     1741 attcatcctc tcgtagataa gtttaattta ttcttgtctc tacgaaaagc tgtctaagat
     1801 ttgatactaa caccaaatgt tgtatacccc caaacaatac acatattttg aaggattagg
     1861 attaataaag ctaaaaatat agtaagtata ctaaatatat ttactatatc tagtatactt
     1921 actatatttt aatcttttga tcgtctatta tatataatcc gttcctcgtc gtttaaagta
     1981 tcaacatagg cgtccattgc aagtttgact atttcataca taggaatatc ttttgcataa
     2041 ctaagatccc gaatggtttt tagtacggga ggatctactt gaactggttt tctttctttc
     2101 gcagtgtact ttcgttcccc taactcgaca ttaatatcat ctttaattga aatatcttca
     2161 gcaccaattt tcttcttatc tttgttcgtt ggtaataatg ccatttagtt taatctcctt
     2221 tccaagaggt aggatatgat tggccattga gtcagcatat ttaggtgtat aatcaaatcc
     2281 ttcaatatca ccatgttgtt catagtattc aattctttct tctaattctg taaagatgtc
     2341 tgcaaaaata gcccaccatc ttcgatctac ataatcattt aattggatac cattctcacc
     2401 atacatttct aaccgatccg agcctttaac aatgctttta aaaatgtttg attcaccata
     2461 cttatcaatt gttttttgat agtttgcgtg ttgagaggaa cgtcgcactg agaaaagaac
     2521 aggcaaaatt ccaatgattt gaagatgaga ctcttcaaaa ttcgtgacaa tcggaactaa
     2581 aactttatta ataaaatctt ctgttccttc cattgcgtac cgttttaatt cttgcattgc
     2641 aataatataa tctgcagcag ttaagaatgc acgaaccaca ttatctgttg atggtgggca
     2701 gtcgataaag atataatcga aattttttct caattcttct aaaggtttaa tgaaggccaa
     2761 tgatctctta cgacgatcag gatatttttc ggacaagtat tcattaagtt cttcagatcc
     2821 aggagagcca gccataatat ataaatgtgg acttacttga gtaatacctg aaacccagtc
     2881 cccttttttg attgctgcag acatggatcg atcaaagcta gtaatttgat acgttgatcc
     2941 aatgttggaa gtagtatttc tttgttggtc ataatcaatt agcaacacgt ctttttcaaa
     3001 aagttgacta gaaacaatcg ctaataaatc attgtctgta gtcttaccaa tgccgccttt
     3061 tctacctgta agcattacaa cttttcctag tttatctgat gttttaaatg cagtccgtaa
     3121 gttttccata gttccctcca atttcctata ctaaatatat ttagtatact tcatatactt
     3181 aatatattcg atatatctaa tatactccaa attgataaga attcagactt tataaggaat
     3241 atagtggata tactaaatat atttgctata ttcagtatat cagatatatt caaaatgtca
     3301 aaatatattt gtgcgttttg cgttgacaat ttagagtgaa aagaggtaaa cttaattcta
     3361 tagaaaccac agataattca ctatttacaa agtagcaaaa gcagtggtac tctttaaaca
     3421 gaaaaattac ataaaaaaaa gagacggaaa taattgattt tagcagaatc aattatttct
     3481 gaatcacatg gttaggacat gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa
     3541 ctacttatat taaaagttga tcggatacct tttccgattg cctttattct accaaaaaaa
     3601 ggcgagcaac tcaacagaag aacgtttatt tagtgtactt aaattaaata ttcttaaaca
     3661 ctgaagagag aacacatact atggctgaga ttaaagctaa actgtgttct ctttttgttt
     3721 gagaaaggac gttacaatcg atgttgagcg aaatttattc agctattctt aaaaacggct
     3781 tacgtacata caaatatcga aacagcacca tgaaacctgc aggtcatatt gattccagta
     3841 ggagtggtgc tatctttgcg taccgtagca agcaattaat gaatgttggg cgtggtatgg
     3901 tcattacttc ggaagaagct attttagaga acgaaaagaa actaacgcat tggacaccta
     3961 atacctatcg ctttggtact tatgcggatg attatcgaac agtggttaaa ggtcattctg
     4021 agaaaaactt aagtcaaatc aacacatttg tagttgatat cgattcaaaa gagaaccatc
     4081 aaggcgaaat tattttagcc tgtttagatc aagtgggcta catgccaacg ctaattttag
     4141 aatcagatca tggttatcaa gtttattttg tactaaagac accaggctat gtcacaaaga
     4201 aaagcaattt caaggtgatc gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt
     4261 tggctgaaaa attgtccgga gtggatctag gatgtaacca cttcggaatt gctcgattcc
     4321 cacaagaaaa taatattgtt tttaaagaaa tgaactatca gtattctttt gctgactgga
     4381 ttcaatggtc gatgaagctt gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac
     4441 taattgtctt tcctgagaaa aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt
     4501 tacttcataa agcaaacatc gtcggcggag aaggacgtct tggtcgaaat aatgtgattt
     4561 tcacgctttc tttagcttat tactcttcag gttatgcatt ggatacttgc gaatacaata
     4621 tgttcacatt caacgaacgg ttacaggaac cgctatctga agcagaatta gtaaagattg
     4681 tacgtagtgc ttattctggt aattatcaag cagcaagcag agaattcatt attgaattgt
     4741 gccagcaatg ggtttcacca accttaaaaa ataatgatct gttcattcaa cgacgtggct
     4801 ggtggaaatt taaaaagcca cgagatcaaa gaaaatatag tcataaaaat gaatggaaag
     4861 aagatctatt ggcttatcta agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt
     4921 caaaaaaaga attggcttca caactaacga tgcccaagcg aacacttgat aagatcttaa
     4981 atgagttacg agaggagaat aaagtcttct acaaggtaac acgaggaaga aatggcggaa
     5041 tgatcattgc ttcagtcaaa acgttgttcg cgagggttat tcaactaagt aaagaaaaga
     5101 gactagatta ttcaacaagg atcacagagg tatttggact caacgagagc ttagtagaag
     5161 agacatttag acaattatca acccccttga accctcttaa agaagtgaat ctttttgaag
     5221 tagatacagg ttgaaaaata aaaggtgcaa attgccttat acaatatatc tatgatactt
     5281 ctgtaggtgt tgctcccttt gcctagctac gtgtaggtgg tatacttata actgcgtatg
     5341 cagtcttatg tatcatggag gaagttgtat gtcgttaaaa aattatgtct attcacatcc
     5401 tgttaatgtg tttatcattg gtcgtttggg attatctgtg gaagtgttct gtgagcttta
     5461 tggttttaag caggggactt tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt
     5521 gccgattgag tttcttcatg ggttgtcctt agcttctggg gagactatgg atcaggtata
     5581 tgattgtttg tgtgtgttag agcaggagta tattgagtat aaaatagcta acgagttaag
     5641 aaaaaggaag aagtatatcc agtagtagtt tttatttact cttaattgtc aaattaagtt
     5701 agacaaatca tcaatactta cgtaactcaa aaatacttcc aaaggtgttc gataattcag
     5761 tgattttcta ggaatattat ttcgtttcga tgcgacagat tgaataaaag attcatctac
     5821 tgaattgaaa tccatagatt tcaataaacc atctctacgt aacaatccgt tagaattctc
     5881 gtttaggcct ctttgagacg gcgttcctgg atcagcgaaa taaatggcaa tatcattggc
     5941 attactgatc tgtttccaat ttgaaaattc ctttccacaa tcaaaagtga tggatttgaa
     6001 tagatttttc ggtacagatt caaaccaatg atttaatttg ttttcaatat caatcgcttg
     6061 tctaccacac ggtttcaatg tgatgataac ttttgataat cgctcaacta aggtaattac
     6121 agcactttta tgtttcaaac ctacgatagt gtcaccttca aggtgaccaa actcatttga
     6181 gaattggcta taatcttttt cacgttcatg aatagagcga cggaaagctt gttttccacg
     6241 tctttcttga tgtccatttg gtttacgctt gcctttcatc ggtaagtcag aaggattaaa
     6301 tagccccttt ttgaacatac gataaatggt acgagcagaa caac
//
LOCUS       DACIRY010000140         6295 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.146, whole genome shotgun sequence.
ACCESSION   DACIRY010000140 DACIRY010000000
VERSION     DACIRY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.146"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(102..482)
                     /gene="yajC"
                     /locus_tag="IYE22_002406"
     CDS             complement(102..482)
                     /gene="yajC"
                     /locus_tag="IYE22_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ9507592.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(579..1724)
                     /gene="tgt"
                     /locus_tag="IYE22_002407"
     CDS             complement(579..1724)
                     /gene="tgt"
                     /locus_tag="IYE22_002407"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ9507593.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1942..2640
                     /locus_tag="IYE22_002408"
     CDS             1942..2640
                     /locus_tag="IYE22_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ9507594.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2808..5447)
                     /locus_tag="IYE22_002409"
     CDS             complement(2808..5447)
                     /locus_tag="IYE22_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ9507595.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6066..>6295)
                     /locus_tag="IYE22_002410"
     CDS             complement(6066..>6295)
                     /locus_tag="IYE22_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HAQ9507596.1"
                     /translation="NGEIKADSGWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSA
                     FAGRELTLSAYQHAIEERYRFFSFGDAMFVK"
BASE COUNT         1779 a         1379 c         1149 g         1988 t
ORIGIN      
        1 attgagcaat atatcccatg ctcttatctc tttctttttt gaataaaaaa tgaggtagga
       61 catctttctg ccctacctcg tctttaaata agctttgata cttatttttc ttccttctct
      121 tctgtctctt ccagcgtttc tgttgtttca gtttttactt caactgtctc agctggttct
      181 tctgttgttt caacagtagt cgttgcatca tttgttacga cagtccctgg tttgaccgtt
      241 ttgattgctg cacgatcgta ctcaaggaag atcccttcgc agtctaaaac aactgtccgt
      301 ttgtcattgt cgatttctga aacaactcca tgtaatccgc cgatcgttac gactgaatca
      361 cctggtttca tgctatctaa caagttttga cgttcttgct gttgtttctt ttgcgaacgt
      421 gacataaaaa accacattgc taatagcgcg ataaacatca caagcattgg taatgttccc
      481 atttttttca cctcactaat tctgtatcta caaaaaacac tttagcagaa aaatgctgat
      541 aacactagtt ttttacttaa tttttaaaag attttccttt agaagctttt tgcgttttct
      601 ttattgaacc cgtattcttc aaaaaaggct tctctaaatt caagcaaatt atcatccatg
      661 attgcttgac gtacttgttt cattagattc aacaagaagt ataaattgtg gtaagaagtc
      721 aaacgaatcc caaatgtttc atcacatttg atcaaatggc ggatataggc acgtgtatag
      781 tttttacatg tataacaatc acatttctca tcaattggac ggaaatcatg ggcatatttc
      841 gcatttttca ctactagacg gccttgagaa gtcatacatg tgccatttcg agcgattcga
      901 gtaggcaaga cacagtcaaa catgtctact ccacggataa caccatcgat caatgaatca
      961 gcagcaccga cacccatcaa gtaacgtggt ttgttttcgg ggatcaacgg tgtagtgaat
     1021 tctaatacac gattcatttc tgatttaggt tccccgacag ataacccgcc gattgagtaa
     1081 ccagggaaat ccatcccgac tagatctttc gcgctttgac gacgaagatc ttcaaaccct
     1141 gctccttgga tgattccaaa taatccttga cggtctggat tagcatgagc tttcaatcct
     1201 cgttctgccc atcgagacgt acgttctact gattttttca cgtagtcgta actttcatcg
     1261 aatggtggac attcgtcaaa actcatcatg atatcagagc ccaatttatt ttggatgttg
     1321 attgcttttt ctggtgataa aaacatacgc gaaccgttca agtgattttt gaaatgcacg
     1381 ccttcttctt cgatattccg catatcactt aatgaaaaaa cttgaaagcc gcctgaatct
     1441 gttaaaatcg gttgatccca gttcataaat ttatgcagtc cgccagcttc ttctactaag
     1501 tcttcaccag ggcgtagcca taaatgatat gtattactta aaataacgcc agcacccatt
     1561 tctttcaact cttctgggga cattgttttt actgtagcta atgttcctac tggcatgaac
     1621 attggtgtgg ggaaagttcc gtgtggcgtg atcaattccc ctaaacgcgc gccagtatgt
     1681 ttttctttct tgattaaacg atagcgaatg gcaggttgcg tcattcattt ccctactttc
     1741 tatataaaat atcttgacaa ctcccaccat cataatcgtt tcaagtgctt tttgcaagtt
     1801 ccctcggctt ccttctactt tatttaatat tctttttttt atcctttttt tatgaataac
     1861 aaaaaaaaga aaaacattat gacagaaata ttttttgata catggtatat tggtgttatt
     1921 aaagaaaagg agggaaaaat catgcaatct tctgcagaat taaaacgcca agcaaaagag
     1981 agtctaaaag gacgttgggg acaagctgtt ttactaaatc taatccctac attgattggt
     2041 attgctacta ttcttcttat tatgattcct attatcttgt ttgccaccac catatatgat
     2101 ggatcagcga gcgtctcaga agcgacaact tcaggaggcg gtagttcagg aggcggtggc
     2161 ggaattattt ccagcatcat tagcgctttg ttcatgagcg gtatttcttg gacttatctt
     2221 gatattttac gtggacaaaa agatcagatc gaaccattca aagatgcatt tcgtggattc
     2281 aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc tgatctcgat ctttacaaca
     2341 ttatggggtt tgctattagt tattcctgga atcattaaaa gctattctta ttctcaaagc
     2401 tattttattt actacgatat cgtccaagaa acaggacaaa cacctaaagt actggatacg
     2461 atcactgcaa gtcgaaaatt gatggatggt tataaaggac aacttttctg gttagatctt
     2521 tcatttatcg gctggcatat cttagcatta gcaactgctg gtattggtta tctatggctc
     2581 agcccataca tttctgcaac aaaagcagca ttttatgaac aattgccaaa agatatttaa
     2641 aagagagtgt gacttgtgac cgagggggtg gcaggagaac accgtttatc gagaggcagt
     2701 aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac aacattcatg tcacacctcc
     2761 tttttgtata gttccagtaa aaagaaaatc aacttagaaa cagttaatca ttcctcttct
     2821 tctgcttccg gtaatcgatg gaacttgatc agttcattca ccgcgattgg tacgatagat
     2881 aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta ctaggccgaa tagttcttgc
     2941 gtaaatggca gcaaagcaac agccgttgta atagctaatg ccagtaacgc catttctacc
     3001 aaagctttat tggtcgctaa attgactttg aagatcgatt ggctgctgcg tacattgaac
     3061 acatgcagaa tcgatgaata agctaagatc aagaatgcaa cagtttgacc gacatgctga
     3121 cttgcgtcta cccatgggct gatagtatcg acataactac ctaaataata accaaacaag
     3181 gttacgatgg tgaagacccc tgcatttatg gcaatttttt gcaataaacc tcgagcaaag
     3241 atcccttcat ttttcggaat gggtggctga tccatgatgc cgtgttccgc tggctctctt
     3301 cctaaagcaa atcctggaag tccatcagca actacattga caaacagtag ttgcacagct
     3361 gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa caataaagat ctccgaaata
     3421 ttacagctta gcaaaaagtt gatagcttta cgaatattct gataaaccga acgtccttga
     3481 ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca aaatcatatc tgcggcactt
     3541 tgcgccacat ctgttcctgt gatccccatg gcacacccaa cgttacttgc tttcaacgct
     3601 ggtgcatcat tcacaccatc cccagtcatc gctacgactg cacccgtacg ttgccaagat
     3661 ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat agacagagag agctttgaca
     3721 cgcgcatcta attcttcgtc tgataattga tgcagttctg atcctgaaag tgcttctgac
     3781 ggatcattca agatacctaa ttctttcgct atggcactgg cagtcaccac atgatctcca
     3841 gtgatcatga ctgttttgat gccagccttt ttcgctcgct tgattgctcc tttactttct
     3901 ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac gtaagtcttt ttccagtgct
     3961 tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat aaccaacagc aatcactctc
     4021 agtgcccgtt taccaaagcg atcattaacg atcgccgctt gttctacatc gcctgaacta
     4081 aagcgcggag caagaacatc gaatgctcct ttggtgattg agatgtattt ttcgcccatt
     4141 tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag gaagctcgtt tactcgtgga
     4201 tatttttctt ctaattctgc ctttgtatga taattctctt caacagccct tacgatggct
     4261 acttctgttg gattaccttt gataacaagg tcgtcatctt cttgctcgac cgtcacatct
     4321 gtacatagtg cagccatttt caatatttcc atcgcgctgt tcgtcattga atcttcgata
     4381 ttcgttactt catcttcttt gctccatacc cggcggacgc gcatttcatt ttgtgtcagc
     4441 gttcctgttt tgtctgagca gataacatta gcggttccta gcgtttcaac tgctggcaac
     4501 tgtcggatga tcgcatgttt cctcgccatc ttctgcacac cgtatgcaag tgtcaacgta
     4561 acaataacag tcaaggtttc cggtacagct gcaacagcca aggaaatcga agtcatgaac
     4621 atctcaagga gtggttctcc ttgcagttca ccaatcacaa atactaaagc cgcagccgct
     4681 aaagcgatca tactgattcg tttgcctagc tgattcaatc ggacctgtaa aggtgttttt
     4741 tgcatagtat tttcgttcaa aagatccgcg atcttaccca tttctgtagc catgccaatg
     4801 gcagtgacga tcgctttccc acgacctgca gcaaccgtac atcctttaaa taccatatta
     4861 agttggtcac ctaatgactc atcttcttca gcaataaatg ctgcatcttt ttcaactgct
     4921 tcactttctc cagtcaacgc cgattcttct acacgtaatt gtgatgcttc gatcaaacgg
     4981 gcatcggcag aaatagcatc accggactct aaaacaagaa tatcacccac taccagttgc
     5041 tctgactcaa cagtggtcac tttgccgtct cgtatgacag ttgccgtttg tttattcata
     5101 tcttgcaagg attccagtgc tttttctgcg tttccttctt ggacaatcgc caataccgag
     5161 ttgatcacaa cgatagcgat gatcagcagt ccttcaaata aatccccatg ttctgtagca
     5221 aatgcggtat aaaaggaaat caccgctgct acaagaagga tgatcgttgt aaaatcggac
     5281 aagcttcgaa agaatttttt gatcatcgat tctttcggcg cctcatcgaa tttattcaat
     5341 ccctgctttt ttcgccgttc atccacttct tgtgaagaca acccagtatc tggatctgct
     5401 tctgttacct tctttacttc ttctattgtc tgcttgtatt cttccatcct attcctcctt
     5461 taaaatcttc gtacaaaaaa aggtacttat tttactataa catccttttt ttatttactt
     5521 caaataaagg gcttatcgat tatttgttaa gaaagcgtaa aatttaaagt gcttatattt
     5581 gcaaacgcta ccaaaggatg ctattatata gatgtaagga aggaggatac catcaaatag
     5641 acagaagaat ttctcgatag aacagtgtaa gcaaacaaag ttgttagaag tacaggtttc
     5701 acccagacat gcgtgtagag tacaaaccta taaatagtga atgcgtatgg atgtttggat
     5761 cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca attgaataac gtaaacgtct
     5821 gaaaattgaa taggaacaag agatgtttca aatgttgaag atgtaacgaa aacaaaattt
     5881 ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt atgatggaaa gttacaatca
     5941 tttctgttta tttgaatcct tccgaggtac aagaaagccg agcttatcga gagctcggct
     6001 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
     6061 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
     6121 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
     6181 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
     6241 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaa
//
LOCUS       DACIRY010000141         6286 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.147, whole genome shotgun sequence.
ACCESSION   DACIRY010000141 DACIRY010000000
VERSION     DACIRY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.147"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            86..520
                     /locus_tag="IYE22_002411"
     CDS             86..520
                     /locus_tag="IYE22_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507597.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(598..1131)
                     /locus_tag="IYE22_002412"
     CDS             complement(598..1131)
                     /locus_tag="IYE22_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ9507598.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1225..2403)
                     /gene="mutY"
                     /locus_tag="IYE22_002413"
     CDS             complement(1225..2403)
                     /gene="mutY"
                     /locus_tag="IYE22_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ9507599.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2396..3193)
                     /gene="recX"
                     /locus_tag="IYE22_002414"
     CDS             complement(2396..3193)
                     /gene="recX"
                     /locus_tag="IYE22_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ9507600.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3300..4682
                     /gene="rlmD"
                     /locus_tag="IYE22_002415"
     CDS             3300..4682
                     /gene="rlmD"
                     /locus_tag="IYE22_002415"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ9507601.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4856..5434)
                     /locus_tag="IYE22_002416"
     CDS             complement(4856..5434)
                     /locus_tag="IYE22_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ9507602.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5756..6226
                     /locus_tag="IYE22_002417"
     CDS             5756..6226
                     /locus_tag="IYE22_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ9507603.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         1861 a         1227 c         1118 g         2080 t
ORIGIN      
        1 attctttttt tatttttgaa aacttgccat actttttatt tgttttattt tccagtatac
       61 tatacgaaag gaaggtggct agaccatgaa agttgtacaa acaaaagata caatgagcaa
      121 tatttactta gatgctgtac ggatcaggca tcaagttttt gtagtcgaac agggtgtgcc
      181 tttgtctcga gaaatagaca aagatgaagc ccattgtatc cacttcgtcc tgtactcaga
      241 taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa aaatgaaatt
      301 acagcggatg gcgattcttt ctgaatacag acaccaaggg ttaggaaaaa tattgattga
      361 agaagcagaa aactttgcta agaatcaagg ctataatacg attttattag gggcacagtc
      421 cacagctgaa actttttacg aaaaactagg atatacagct tacggcgatc cttttgaaga
      481 tgctggcatg cctcatattt atatgaaaaa gactttataa aaatttattt cgaaaaaagc
      541 aaaaaacagc aagcttttga gaaaaatctc tcactggctt actgttcttt tttgtgttta
      601 cttacgcgac aattgcttgt aacggttata ccaaatatca atatagcctt ccgagaacgg
      661 gccatctcca ttattgatcc agtcaaccag aatcttcaca ttttctttta ggatgaagtc
      721 aatatcgttc gggtaattca tcattttgct gtgcatctca tattcatcga catccaatag
      781 tcgtttctcg ccgtcaggaa ataccttgat atccaagtca tagtcgatat atttcaaggc
      841 ctcatcgtcc agcaaaaaag gtgaagctag attacaataa taggaaaccc ccttttctct
      901 gatcattgct attacattga accagtattt tttatgaaaa taaacaatcg ctggttcgcg
      961 cgttacccat cggcgtccat cagattcagt gactaatgtg tgatcattca cgccgatcat
     1021 agagtgttcg cttgttttta ataccatggt atctcgccac gttcgatgca atttgccgtc
     1081 gtgcttgtaa ctttggatcg tcacaaactc tccttctttt ggaactccca tagcaaccct
     1141 tccttaagca gcattttgtg ctttgtgttt gggtgttcat tacaccgaca tcataattat
     1201 tatatcaata aaaatgagct ttgcctagaa aggattccta ttgtttcgat ttttctctaa
     1261 ctgatctacc agtttcattt ggaccttagg aaaaacgacc gaatcaaatg catcaggctt
     1321 caaccacttc gaagttttgt tctcggtaaa ttcttgttct gctccctcgg ttgcccgtcc
     1381 gtaaaaaagg agaacatgcc atttcagatg actaaaaata tgagtaactt ctcctaaatg
     1441 tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg agattttgct cttcttctgc
     1501 tactaggtca tcaaacagat cgatttctgg ctttgtttcc cattcttttt tcaatcgctc
     1561 atactcctct tgtgtcactt ccatcattgg aaatgtccac atattcgcca gcaatttttg
     1621 gctatctctt tcttcaaaat aatacgctcc agaatgattt tgaagagctg cactgatata
     1681 ataaacgtct tttggctttg ctttttttgt ttttacagga aaactcgttt ggattcctcg
     1741 tttgttagct aaacaaaagg cttggatcgg acaagtctca cactttggag aagttggtgt
     1801 acagatagct gaacctagat ccatcattgc ttggttgaat tcacctggat gcttttcatc
     1861 aatgattttc cgcatcgctt catcaaagat tttcctgcta gaagcttttg cgatgtctgc
     1921 ttcaatgcaa aacaatctgc tgactactcg catcacgttg ccgtcaactg caggttctgg
     1981 aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat ggtcctattc ccttcaatga
     2041 gctaatttct tcaggcgttt gcggcatttc cccatcaaat tctgacatga tctgtttagc
     2101 agcagcttgg atgttccggg ctcttgagta ataaccaagg ccctcccatg cttttaaaag
     2161 cttttcttct ggagcatttg ctagctcttc aattgtagga aaccattcca tgaaacgata
     2221 aaaataatcg atcactgtgt ctactctggt ttgctgaagc atgatctcag agatccagat
     2281 acgataagga tcacgattat agcgccaagg caggtttctt ttttcttgtt cataccattg
     2341 aatgaactga tcttgaaatt cttttgtttc tttatctgtc catttttccc attctttatt
     2401 cactttcctc ttccttttcc gtaatgaatc gctggatcat atccaaatca aatccttttt
     2461 gatataagct ttgctttact tttagatttc tttttttagg ttcaaacctt aaatttcttc
     2521 gccatagttt atctccttgt ttgactaaat aatcatattc tatttcttga tctgcttctt
     2581 gcggaagatc agctatcgct tgttggatca cttcttgaga aaagcctttc gtcatcaatg
     2641 tttgttggat ttttctcaat aattcttttt ggctttttcc gtgatttttt ccaaacgctt
     2701 tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa tgtatattgg ctgagcgctt
     2761 gttcaatgat ttcaggtttc aaaccttttt gctgcatctg ctgcgctaat ttctttggtc
     2821 ctttgtcact taagcgcata ttcgtgcgca cataactttc cccatagacc aaatcatcga
     2881 caaggtccaa ttcttttaag cgggctacga ttttatttcg atcttcttga aggatttcct
     2941 tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa ctgataactt aagtaattca
     3001 ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt gatctcttgg atagtttctt
     3061 cgtctaattc ccgtcctttt aataatcgat agcgtactaa tacatcttca gatacttgta
     3121 aagcttctcc ttcagataaa tctacgcgat agaaagggcc tttgccttta ctgattctcg
     3181 taacggttaa cataaacatc ctcctcgaac atatattctc tatcatacca cagaaaatcc
     3241 ccgtaatcag gtattatatg ttagaataaa ccacagaaaa aagaaaaagg gagatactta
     3301 tggcagaaac aattgtcaaa actggacaaa cggttccttt gaaaatcaag cgtttaggca
     3361 taaatggtga aggaatcggt tatttcaaac ggttgatcat atttgttcca tatgcactac
     3421 caaaagaaga agtactagtg aaaatcacga aagcaactcc tcgctatgca gaaggagaat
     3481 taataaaagt caaaaaaaca agcaaagatc gcgtagtggc accatgtcct gtttactatg
     3541 aatgcggtgg ctgtcaatta caacacttag cttatcaagc tcaattggat tttaaaaagg
     3601 atctgctttt gcaagcattg gaaaaattca aaccagcagg ctttcgatct tatcaacttt
     3661 tgccgacgat cgggatggat gagccttggc attatagaaa caaagcacaa tttcagttac
     3721 gaaaaaataa gcagactcaa aaaattgaag ctggtttata cgaagcaaac tctcatcagt
     3781 tagtccctat aacagactgc ttggttcaag aacctcgaac acaaaaagta atgaatactg
     3841 ttgttcaact attgaataaa tttgatgcac ccatctatga tgaacggaca aacagtggaa
     3901 tcttccgcac aatcatggtt agaatcggtg ttaaaactgg ggagttacaa gttgttttta
     3961 ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat ccgtgagatc aatgaacgac
     4021 tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca aaaaacgtct ctcgtgatgg
     4081 gggatgaaac gatccactta tggggaaaag aagccattga agaacaaatc aatgaagtga
     4141 ctttcgatct ttctcctcgt gccttctttc aactcaatcc tcagcagacc gaattgcttt
     4201 atggcgaagg aatcaaagca ttagatgtcc agcctaatga aacgatcgtg gatgcttatt
     4261 gcggagttgg gacgatcggc ttaagtgtag caaaacaagc caaagaagta cggggaatgg
     4321 ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc ggaaagatta ggatatcaca
     4381 acactcgata tgaagtcggg acagcagagc aagttctgcc taaatggttg aatgaaggtt
     4441 ttcagcctga tggtatcatc gtagatccac cacgtacggg tttggataac gcattgatcc
     4501 aatcattatt gaaataccct ccaaaaaaac ttgtttatat ctcctgtaat gtttctacac
     4561 ttgccagaga tctggttaat ttaagtaaaa agttcaagat tgaatattta cagtccgtcg
     4621 acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa gatgacgaga ctcgctgact
     4681 aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt aataagaaca gccaaacaaa
     4741 aatagctctt catgttttat gaatactgta cttgtgaccg aagagttaac aggaggacac
     4801 tatttattta aaaaaagggt tgtgacatag tttttgtcac agcccttttt gattattatt
     4861 ggatattcac gtctatcact tgataaaatg catttgtcgt atcgctgata ttccatactg
     4921 ctaaaatcac attatatcct tttcgatcct gcggaagtgt gatcgtttgg ttgactgttt
     4981 ttggtggaac agttgctttg tcgtctattt ttgtgatcaa ttcaaaatca ctaaatttca
     5041 aaggttgatt aggattccaa gttggtttgg taatgtagta atcccaagta cttgtccgat
     5101 gttgtgctgt taaattccaa gtaatattta atgttcctgg attaaccatg tttttatgcc
     5161 atctgttaga tgtctgttca tctaaaggct caaagccgct gactcctgca ctagcaattt
     5221 ttccatcaat gaatgtattt ttggtagctt cgatactttg cggttcccat tgtgctcttc
     5281 cgacattttg atttaagttt cctgctgctg atgttcctag gtatgcacgg cttcctggag
     5341 aagtaatata accatgcgca aaacctggaa tgctaaaacc gaacaaccct aaactaacta
     5401 acccaataaa caataacgta ctaatctttt tcatgtagaa acctccttca tttgataacc
     5461 acattgtaaa cgctttattg tattaacaca aacattatgc ttttataatt ttatgttgaa
     5521 gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga agtagacgag aaaagtacaa
     5581 atagaaaaca tgagaatcaa atcatatgat ctttttcaaa aacatcattc atataaacaa
     5641 atgttcaact ttttaataac aacaattatc aatttttttc ttacttcaca aactaaatac
     5701 aggtcttgct gtgagccttc atggtaaact aatattatag aaaggagtta gtaaaatgaa
     5761 gatgataaaa gatattcgtt tttgggtttg tgttatagga attgtcatat taggcttttt
     5821 atcagggttg cttagcggaa atcccggaaa atattactat tctttgcaac ttccaccatt
     5881 tgcaccacct agctggatat ttgggccgat gtggacctta ctgtatatat tgatgggtat
     5941 ttctctttat ttgttactta atcacaacaa caaaaaacag cgaaacaatc ttgtcggtct
     6001 ttttgtgatt cagttcattt ttaattttat atggtctgct ctatttttta atttaagaaa
     6061 tattttcatt gcagcaatcg atattacact tttagttatt ttcttgtcag tgcttatgta
     6121 tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg attccttatt atttatgggt
     6181 actattcgca actctattga attattcaat ttatttttta aactaagcgc taaaaagaga
     6241 cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt tagatt
//
LOCUS       DACIRY010000142         6118 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.148, whole genome shotgun sequence.
ACCESSION   DACIRY010000142 DACIRY010000000
VERSION     DACIRY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.148"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(195..482)
                     /locus_tag="IYE22_002418"
     CDS             complement(195..482)
                     /locus_tag="IYE22_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ9507604.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(502..726)
                     /locus_tag="IYE22_002419"
     CDS             complement(502..726)
                     /locus_tag="IYE22_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ9507605.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1366..1899)
                     /locus_tag="IYE22_002420"
     CDS             complement(1366..1899)
                     /locus_tag="IYE22_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ9507606.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1920..2864)
                     /locus_tag="IYE22_002421"
     CDS             complement(1920..2864)
                     /locus_tag="IYE22_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ9507607.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3501..3587
                     /locus_tag="IYE22_002422"
     CDS             3501..3587
                     /locus_tag="IYE22_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ9507608.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3708..4397)
                     /locus_tag="IYE22_002423"
     CDS             complement(3708..4397)
                     /locus_tag="IYE22_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507609.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4404..4682)
                     /locus_tag="IYE22_002424"
     CDS             complement(4404..4682)
                     /locus_tag="IYE22_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507610.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4642..5556)
                     /locus_tag="IYE22_002425"
     CDS             complement(4642..5556)
                     /locus_tag="IYE22_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ9507611.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5538..5918)
                     /locus_tag="IYE22_002426"
     CDS             complement(5538..5918)
                     /locus_tag="IYE22_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ9507612.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1615 a         1135 c         1036 g         2332 t
ORIGIN      
        1 cgcttgtttc accttcggtg aaattgttgc aaatcaataa ttaggtagag ctacattttt
       61 ttcgttgttc aaagattctt atgccattta ctattaataa atctataaat atgataattt
      121 ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagtc ccattttcat
      181 tttattccaa agaattaact ccaaatacca atagaagccc atcctctata tacttgagat
      241 tccttactaa caatttcttg taattctatt aaagatttag gtaatccaaa tttatgtttt
      301 aaaatatatc ttccaagaaa actacttaat ttcatactaa cttcaccaac actttttccc
      361 tcactgattt ctttagaata aagaaagagc ttttctttta agtcaggata gtcatctata
      421 gttgcttcat tgtatacttt actaattgca tttaaaagtt tttcttcttt agtaaaatcc
      481 attataataa cctccctacc actaccttct aggtgcccat ggaccatgat taacccaacc
      541 attaacaata atttttccaa tttctccttt agcttgattc caatctaccc aacatttttc
      601 tttgttacaa taaagaccat ttccatagta agtagctgca tcaactttta ttcctgtacc
      661 gatgccagct agacaagttc ctaatattcc tagaataaca caatgtttta atactttctt
      721 tttcatgatt gtttttcctt ttcgtttttt taaaattgct tgttatatta aataatctta
      781 gtaattttat tatataacat tttattatat aacaatgatt aacgtcataa aaagtagttt
      841 tgaattcata atcgtttaaa taaaattata agatcagtta tttaaacagc tagtagacat
      901 aatgtgtata ctaccaagta acgcctgtac caagggatcc aggcgctgat tttgtgaatt
      961 tttattggtc taggttaaaa gctcttttta aggcttggtt ttctgcggtt gtttctttct
     1021 aattctttgt gaatattgat cggtcatttt ttaaatactt gttatttttg tttgtgtttg
     1081 ttatgctttt tacatatctc atattcattt attattcttt tctaaggtta ataataggat
     1141 aatctctttc agaaagactg ctcgtacgag cagtcttttc ttatttatca agtttttcta
     1201 caaatacttc ctcattttga ctgttgtagt aattaggatt gagttttagt tccccttttt
     1261 tatttccttt gttggccact gataataaca ttgggctaaa gcttaatatt cctagtagga
     1321 tagaaccgaa tagcaagtgt ttttttgtca taagtatttc cttcttcatt tccatagctg
     1381 ccaccatttt ttccaattct ttgtttgttc gtccattgtt tctgttgctt cagaaagagt
     1441 cgttgaacta ttctctgaag actcttccgt ataagtcaac tgcagttgct cctgaagttt
     1501 ttcgatttgt tggttggctt gcagggtgag ttgttgttgc tggtcgagta gtttttgtat
     1561 atgagaatta ttttgctctt tgagggcaag ttgttctcgt aaaaattcat tttcttcacg
     1621 caactgatct agcaacttgt ttgcaaccac gtttttgttt ggtatataag catttttatc
     1681 gttgttttgt tcatgtttat gcaacttatc tagcaacctt tgattgtcta agttgcgagc
     1741 aagttgcaat actccagaac gcaatacaac cttataacca cgctcatttt tagctatcat
     1801 ggaagaatct aatttttgga tatgtttcga tattgcttgc ctactcatct taaaaagatc
     1861 tgacaattcc tttattgtaa aggtttcttg tggttgcata ctggttgcct cctaggttgc
     1921 tactggaata aattatctaa atcagataat aaactttttt tctctttttc cgaaagtgcg
     1981 gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat ttgttggagg
     2041 agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc gggttcttcg
     2101 tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt ggttggtgca
     2161 tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg atagccaata
     2221 acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt gaagattgca
     2281 gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg cttataactt
     2341 ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa acctttggtt
     2401 ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag ctcgttgaat
     2461 tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag tgctttttcg
     2521 taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa ttctgtaaac
     2581 attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg taaagataag
     2641 attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt tgctttgtag
     2701 ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc tttttcacgc
     2761 attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt tcttaaagga
     2821 attgtattta attcatggtg gtattttaca atctcattag ccatataatc acctcaatcc
     2881 aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca aacagacaaa
     2941 atgtccttca taaacagaca aaatgtcctt cataaacaga caaaatgtcc ttcataaaca
     3001 gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac agcttacggt
     3061 aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta attttcccgc
     3121 ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac tgccaatcca
     3181 aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac attgtactgc
     3241 gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt tgagcggttc
     3301 tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat ctagctttaa
     3361 agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga tacttaaaaa
     3421 caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga cagtcttgcg
     3481 actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt atagaagttt
     3541 gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc
     3601 gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtatcta
     3661 atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca attttgctgt
     3721 tgctgatcta accattcctt gacacttttt agataattta atgccgtttc ttttttcgtt
     3781 ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta ggtaactgat
     3841 tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc actgaataaa
     3901 ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc
     3961 tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata aagacgagtg
     4021 agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt
     4081 tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc ctttttcttt
     4141 ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact tcttgaaata
     4201 cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa tgtaaatata
     4261 acccctggca ataaaaacct cttaaatcga attttagagc cttttaatga atatcttgtt
     4321 tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat gagtagatag
     4381 ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc taacagtgtc
     4441 atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat tacttttttc
     4501 tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg tgaatcaatt
     4561 gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt ctttatttca
     4621 ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg ctcctctttc
     4681 aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag tcgtaagagt
     4741 tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag ctcttcgtag
     4801 ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat gtaaaatcgt
     4861 ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt tgtggatcaa
     4921 ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt taagagaatc
     4981 acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg
     5041 aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag acatggttcg
     5101 tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac aaatctcgtc
     5161 atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt tgttcccagt
     5221 ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt ctgtatgtgt
     5281 gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc ctagttgatt
     5341 ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt gtgcttgtac
     5401 acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg cataatcaat
     5461 gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga tcgctcggct
     5521 acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc cttttgcatg
     5581 atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg ttggtgtgtg
     5641 ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc gcgcgcgatt
     5701 gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct
     5761 ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag ctttgcaaat
     5821 tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta
     5881 gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct ttcagtacgt
     5941 cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa gtaaagctca
     6001 ctaaagctca ctaaagggct tggcaagctc tatatttgct agccaaatac ctccgtattt
     6061 ggtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc ctcaaaac
//
LOCUS       DACIRY010000143         6078 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.149, whole genome shotgun sequence.
ACCESSION   DACIRY010000143 DACIRY010000000
VERSION     DACIRY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.149"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="IYE22_002427"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="IYE22_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ9507613.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="IYE22_002428"
     CDS             complement(2204..4390)
                     /locus_tag="IYE22_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ9507614.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IYE22_002429"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IYE22_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ9507615.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="IYE22_002430"
     CDS             complement(4612..5052)
                     /locus_tag="IYE22_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ9507616.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="IYE22_002431"
     CDS             complement(5126..5665)
                     /locus_tag="IYE22_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ9507617.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1576 a         1369 c         1068 g         2065 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagg
//
LOCUS       DACIRY010000144         6004 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.150, whole genome shotgun sequence.
ACCESSION   DACIRY010000144 DACIRY010000000
VERSION     DACIRY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.150"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            337..2502
                     /gene="acm"
                     /locus_tag="IYE22_002432"
     CDS             337..2502
                     /gene="acm"
                     /locus_tag="IYE22_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ9507618.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PSNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2774..3259)
                     /locus_tag="IYE22_002433"
     CDS             complement(2774..3259)
                     /locus_tag="IYE22_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ9507619.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3739..4380
                     /locus_tag="IYE22_002434"
     CDS             3739..4380
                     /locus_tag="IYE22_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ9507620.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4399..5034
                     /locus_tag="IYE22_002435"
     CDS             4399..5034
                     /locus_tag="IYE22_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507621.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5052..5867
                     /locus_tag="IYE22_002436"
     CDS             5052..5867
                     /locus_tag="IYE22_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9507622.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         2140 a          932 c         1220 g         1712 t
ORIGIN      
        1 tagtataaac taataatgtt aattaaaagt gtattttatg aaactatcgt tatttattaa
       61 tgttgcattt ttgtaagtta aaaattggaa atatagattg aaaaaatgaa agcaataagt
      121 aaaataaaat gaatatatgc gaaaaaataa tttacattag gaaaaacctt gtaaaaacag
      181 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
      241 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      301 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      361 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      421 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc atcgaatatt
      481 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      541 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      601 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      661 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      721 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      781 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      841 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      901 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      961 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
     1021 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
     1081 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
     1141 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1201 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1261 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1321 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1381 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1441 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1501 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1561 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1621 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1681 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1741 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1801 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1861 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1921 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1981 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     2041 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     2101 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     2161 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2221 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2281 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2341 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2401 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact
     2461 atctgtgctg ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt
     2521 tgttaaatat gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca
     2581 tttttatata tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa
     2641 gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa
     2701 gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa
     2761 aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct
     2821 tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta
     2881 cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt
     2941 gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt
     3001 cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg
     3061 cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata
     3121 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
     3181 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
     3241 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
     3301 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
     3361 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
     3421 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
     3481 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
     3541 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
     3601 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
     3661 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
     3721 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
     3781 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
     3841 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
     3901 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
     3961 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
     4021 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
     4081 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     4141 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     4201 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     4261 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     4321 actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     4381 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     4441 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     4501 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     4561 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     4621 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     4681 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     4741 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     4801 ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg
     4861 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     4921 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     4981 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     5041 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     5101 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     5161 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     5221 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     5281 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     5341 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     5401 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     5461 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     5521 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     5581 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     5641 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     5701 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     5761 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     5821 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     5881 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     5941 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     6001 gttt
//
LOCUS       DACIRY010000145         5947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.151, whole genome shotgun sequence.
ACCESSION   DACIRY010000145 DACIRY010000000
VERSION     DACIRY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.151"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            48..272
                     /gene="nrdH"
                     /locus_tag="IYE22_002437"
     CDS             48..272
                     /gene="nrdH"
                     /locus_tag="IYE22_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ9507623.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            431..2590
                     /gene="nrdE"
                     /locus_tag="IYE22_002438"
     CDS             431..2590
                     /gene="nrdE"
                     /locus_tag="IYE22_002438"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ9507624.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2640..3605
                     /gene="nrdF"
                     /locus_tag="IYE22_002439"
     CDS             2640..3605
                     /gene="nrdF"
                     /locus_tag="IYE22_002439"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ9507625.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3694..4833)
                     /locus_tag="IYE22_002440"
     CDS             complement(3694..4833)
                     /locus_tag="IYE22_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9507626.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5142..5855)
                     /locus_tag="IYE22_002441"
     CDS             complement(5142..5855)
                     /locus_tag="IYE22_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ9507627.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
BASE COUNT         2029 a         1136 c         1055 g         1727 t
ORIGIN      
        1 tactatctat tatagataat aagaatgtgt tagaggaggc actcaccatg aacgtaaaaa
       61 tcttttctaa aaataattgt atgcaatgta agatggtcaa acggttttta acagaaaatc
      121 aaattgcttt tgaagaagtc aatatcgatg aacagcctga agcagtagac tggttgaaag
      181 cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc agaaacagtt atcggattcc
      241 gtcctgatca gttgcgtaaa cttgccagct aacatgacta aaagaatcaa aacgtgatac
      301 atggatttga agggcttgag acagcaccag tttttgtcgc aaacttccaa atcctttttt
      361 aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca tcaaatacga aatgaaggtt
      421 gggcgtacgt atgagtctta aaaatttaca agatgtcagt tatttcaagc tgaataatga
      481 aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag gataaagaag ctttagttgc
      541 cttttttgag gaaaatgtca aaccaaatac gatgactttt ccatctatta tggaaaaaat
      601 caattacttg atcgatcaag aatatataga agcagaattc attcaattgt attcaccatc
      661 atttattgaa aagctatatc aatttttgga cgagcaacat ttttctttca aatcttttat
      721 ggctgcttac aaattttatt ctcaatatgc gttgaaaacc aacgacggtt ccgcttatct
      781 ggaaacttat gaagatcgag tagccttcaa tgcgctttac tttgcgaacg gggatgaaga
      841 attagcactt gctcttgcag acgaaatgat ccatcaacga tatcagccag ctacaccttc
      901 tttcttgaat gctggacgta aacgtcgggg cgaattggtg tcttgtttct tgactcaagt
      961 cactgacgat atgaactcta tcgggcgctc aatcaactct gctttgcaat tgtcccgtat
     1021 tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa gccggcgctc ctatcaaagg
     1081 atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa ttattcgaag atagtttcag
     1141 ctattctaat caattaggtc agcgtcaagg agctggcgtg gtttatttga acgttttcca
     1201 tccagacatt attgctttcc tttcagcaaa aaaagaaaat gcagatgaaa agattcgcgt
     1261 aaaaactctt tctcttggcg tgatcgtacc tgataaattt tatgaattga cacgtaaaaa
     1321 cgaagatatg tatttattta gtccatatag tgtcgaaaaa gaatatggtg tcccattctc
     1381 ttatgtaaat attacagctg agtatgatca tctagttgca aatccaaaca ttcgcaaaca
     1441 aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa ttacaacaag aatctggcta
     1501 tccttacgta atcaatattg atacagcgaa ccgcgagaat ccaatcgatg gcaagatcat
     1561 catgagtaat ttatgttcag agattttaca agttcaaacg ccttctgtga ttaacggcaa
     1621 acaggaatat gaagtattag gaacagatat ctcatgtaac ttaggctcga caaatatcgt
     1681 gaatttgatg caaagcccta actttggtca atctgtgcgt acgatgactc gtgcattgac
     1741 atttattaca gatgcatctg atatcgatgt cgtaccaccg atccaaaacg gaaataagct
     1801 gaatcatacg attggattag gtgccatggg cttacacact tatcttgcaa aagaacaaat
     1861 ggaatatggc tcagaggatt cattagactt tacaaatatt tacttcatgc tgttgaatta
     1921 ttggacactt gttgaaagca acaatatcgc tcgtgaaaga aaacaagttt tccataattt
     1981 tgaaaaatca gcttatgctg acggcactta tttcgaaaaa tacgtaactg gagaattcca
     2041 gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt tttatcccaa ctgctgaaga
     2101 ttggaatgct ttgaaacaag cagtaatgaa agatggactg tatcatcaaa atcgcttggc
     2161 agtagcacca aatggctcaa tttcatatat caacgatacg agtgcaagta ttcatcctat
     2221 cactcgcatg atcgaagaac gccaagagaa gaaaatcggt aaaatttatt acccagcgcc
     2281 ttatctatcc aatgagacga ttccgtatta cacttctgct tatgatatgg acatgcgtaa
     2341 agtaatcgat gtttatgcaa cggcacaaca acacgtcgat caaggcatga gtttgacttt
     2401 attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg aagacaacaa cgaagcaaac
     2461 gacacgtgat ttgaatattt taagacatta cgctttccac aaaggaatca aatccattta
     2521 ttacgtacgt acctttactg atgatgcaga agaaatcgga agcaatcaat gtgaaagctg
     2581 cgtgatctaa tatcatagat agattcgctt attcatattt taattaaagg agcattctca
     2641 tgtcagaaac atattatgaa gcaataaact ggaatgaaat tgaagatatc attgataaat
     2701 ccacttggga gaagctgaca gagcaatttt ggctagatac acgtatccca ctttcaaatg
     2761 acctagatga ttggcgtaca ttgtctcaat tggaaaaaga tacagtagga catgtatttg
     2821 gcggtttgac actgctagat actgtccaat ctgaaagtgg catggatcag ttgcgtaaag
     2881 atgtccgcac tccccatgaa gaagccgttt tgaacaatat tcaattcatg gaatccgttc
     2941 atgccaaaag ttattcttct atttttagta cactgaatac gaaaaaagaa atcgaagaaa
     3001 tttttgactg gacaaatacg aatccttatc tgcaaaaaaa agctgagcgt atcaatgaaa
     3061 tctacaaaca cggtacaccg ctagaaaaga aaattgccag cgtcttttta gaaacattct
     3121 tgttctattc aggtttttat actcctcttt attatttagg aaacaacaaa ttggctaacg
     3181 tagcagagat catcaaacta atcatccgag atgaatctgt acatggaacc tacatcggtt
     3241 acaaattcca attaggattc aacgagttac ctgaagcaga acaagaaacg ttaaaagact
     3301 ggatgtacaa tcttctttat gagctttacg aaaacgaaga acgttataca gaagaattat
     3361 acgatccaat cggctggaca gaagaagtca agacattcct acgctataat gccaacaaag
     3421 cattgatgaa cctagggatg gatccactct ttccagatac tgccaatgat gtcaacccaa
     3481 tcgtaatgaa cgggatttca acaggcacaa gcaaccatga cttcttctca caagtcggaa
     3541 acggctattt gttaggtcat gtagaagcga tgaaagacga cgattatttg attggattgg
     3601 aataaggatt tattttaatt caaaaatttt tcgaaaaata aaaagagttt agcgtacaga
     3661 tattttaaat gtacactgaa ctctttttta tgcttattca cttgtttctt ccagcacttt
     3721 tggactgtct aacttttccg aaacatgttc cattcttctc aataaataga ttttccggat
     3781 atttaatatt atcgtcttaa tcactgcata gacaggtatg caaatcagca tgcctaatat
     3841 tcctgcgaca cttcctgctc caatcaatac caagatgatc gtcaaaggat ggatattcat
     3901 tgatttccca aataacaacg gcttgatcaa atttccatct aattgttgga tcactaaaat
     3961 cgacaacgcc atatacaaag cttgaaatgg agagataaac aaaccgacaa tgattgctgg
     4021 taccgctcca ataaaagggc cgacatacgg gataatattc gtgattccgc aaatgaccgc
     4081 aagcaacaag ctataaggct gatcgaatat tctcattgcc aaaaagctca ttacaccaat
     4141 aatcaaagca tctaagatcg tactgctgat ataggcagat aatgtttcgt ttaattcttt
     4201 taccgtttgg cgtaactctt tacgaatcgc tttcggaaag aagtgagaca acgcatcaat
     4261 aaatttgtgc ccatccttga acataaatag caaaataaat ggaacggtaa ataacaaaat
     4321 aaaaaattgc atcataaatg aaatgatttt tgagacactt gcagataaac tgactaatat
     4381 gatattgaac aaattactaa gagataaatt tgctgatgct aattgttctt ccaaattgaa
     4441 atttttaaac tcttcccgct gcgccaattc atttaaccat ttgcttgttt catctgcata
     4501 cattggcaag ctctgggtca attgaattgt ctgttccatt aactgaggca ctacattcat
     4561 gacagttaaa acaactaaaa tgattacgac caaaaacgtc aataaaaaac cgaacactct
     4621 cgggatccga tgcttttcga gaaaaacaac gattggatcg aacatataat agataaaacc
     4681 agcaacaagc aatggtaaaa aaattgccga aagcattttc aatattggac taaaaatatt
     4741 cggcatttgt aaaataaagt acacgccgat gatcgtgagt actagctcca ctgtccagaa
     4801 aaataatttt gattgtttaa aacgtgattg catacatctc ctccaaggtc ctttgatact
     4861 tctattctat catttaacaa agagttacgc gctgagattt tcatttgctt aaaaaaaaca
     4921 aaaaaaataa acatatttta atgagtagac gagtacttaa acaaaaacac ggtttggagt
     4981 agtgacaaaa attatactgc agtccctttt tccaaataaa cggtgttcaa aagcttgcat
     5041 cttcgtaata agcccagcca aacgaaaaat ataaggaact atttttattt ggctgggctt
     5101 attacttgat gtaggacatc tattttacaa cctcagatca tttattgcgc aatactgact
     5161 aaatgcaact ttgtgatatt tttgtgtacg gacaactcct cgatcacatc ggcataagtg
     5221 agcccttttg gaagatcaat cgtatagata tttttgtaca ctcggtagtc atcaacgaat
     5281 tttacgtcaa aattcacatc ttcgattacg atatttttct cttcaaaata atcggtaaga
     5341 aaatctttgg tttcttttct atggataaat tggatttcta actttttagt cgtaggtaca
     5401 tggatcacgc gtttcactac tgtgagtgca atagcaatcc cgataaaact gatgatcgca
     5461 atggaatagt aacccatgcc gatggcgatc cctaatccag caattgccca taaagaagcg
     5521 gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga tcgttcccgc gcctaagaaa
     5581 ccgacaccac tcaccacttg agcaatcagc cttgcttcat ctgaacgaat cgttccagca
     5641 agctgtggat actctaaagc atctcttaat gcaccactag cgatctcaac ttggatcaat
     5701 gcaatgatac atgctcccat acaaactaaa atatgcgtac gcatacctgc tggacgattt
     5761 ttatattgtc gttcaaaacc gattgctccg ccaaatagca tagcgatccc taaacgtaaa
     5821 ataatctctg gaattgtcaa taccatatca ttcatctaaa caccctccag tccaatatat
     5881 catgtctgaa aggagcagac aaaaaaagtg caatttttac ctcttacgaa aaaaggactg
     5941 tgacaaa
//
LOCUS       DACIRY010000146         5925 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.152, whole genome shotgun sequence.
ACCESSION   DACIRY010000146 DACIRY010000000
VERSION     DACIRY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.152"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(76..1170)
                     /locus_tag="IYE22_002442"
     CDS             complement(76..1170)
                     /locus_tag="IYE22_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ9507628.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IYE22_002443"
     CDS             1359..2120
                     /locus_tag="IYE22_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9507629.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IYE22_002444"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IYE22_002444"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ9507630.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IYE22_002445"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IYE22_002445"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ9507631.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IYE22_002446"
     CDS             complement(3760..4773)
                     /locus_tag="IYE22_002446"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ9507632.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IYE22_002447"
     CDS             4938..5858
                     /locus_tag="IYE22_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ9507633.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACIRY010000147         5811 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.153, whole genome shotgun sequence.
ACCESSION   DACIRY010000147 DACIRY010000000
VERSION     DACIRY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.153"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1562
                     /locus_tag="IYE22_002448"
     CDS             <1..1562
                     /locus_tag="IYE22_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ9507634.1"
                     /translation="QAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRNS
                     RPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRTE
                     SFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPDQ
                     WEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKMQ
                     LHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYFT
                     NSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDDP
                     QTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPIIE
                     KKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSVQ
                     DFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLGT
                     GSTFTVTIPKM"
     gene            complement(1665..4136)
                     /locus_tag="IYE22_002449"
     CDS             complement(1665..4136)
                     /locus_tag="IYE22_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ9507635.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            4409..5683
                     /locus_tag="IYE22_002450"
     CDS             4409..5683
                     /locus_tag="IYE22_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ9507636.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         1761 a         1192 c         1096 g         1762 t
ORIGIN      
        1 tccaagcagc gcaaaacaaa actgctttgg aagaattcgt ccatcagtcg aacgaacgga
       61 ttactttaat ggatgctact ggaaagatct tatttgacac aaacaatgaa acattgaatg
      121 aacagcgaaa ctcccgtccc gaaatcaaag ccgtattgaa tggtgggaac ctaggtagcg
      181 ctttgcgcaa aagtactact ttgaatgagg atcttttata tgtcgcatta ccagtcaaaa
      241 aaagtggcca gttagaagcc attttacgta tcgcagaacc gacaagcgtt tttttaccga
      301 gaactgagag ttttcgccgt tgggtctttt tcttctttct gtctttcttc cttgttcttg
      361 caggaatgat ctattatctg atctatcaaa aaaaccagcc attgaaaacc gtgctgcccg
      421 tactaaaaaa aatggtgcaa aatcctaatc aggcggaagt aatcatgcaa acacccgatc
      481 aatgggaaga attgtatcag acgattaatg cgttgagcga acaaatgagt aagatgtatc
      541 gggcgtgtac taccacagaa gagcaattat atacgttact gaacgaactg atgattggcg
      601 tattcctgat tgatgatgct gattctcgtt tactactgct taatccaaag atgcagctac
      661 atttaaacgt ggtaagctat caacctgaac agcggtacac agaagtcatt caagagccaa
      721 aactcattca gctgattcat caagtgactc ctgagcatcc gctgatccat caggagatca
      781 cccttacaga aggtcggcag acattagact tgagtttgcg ctactttact aattcagaag
      841 gtaccggaca gattctaggg gtggcgtatg acttaacgaa agtgcgacgt ttagaaaagc
      901 tacaaaagga tttcgttggc aacgtctctc acgaattaaa aactcctgtg acttcactga
      961 ttggttttac cgagacttta ctggatggtg caaaagatga cccgcaaact ctgacttctt
     1021 ttcttgagat catgcaaaaa gatgcgatcc ggttagataa actgattcgg gaaatcatcc
     1081 agctgtcaaa agatggcgag aattcttacg agatccagac aatttataca gaaccttatt
     1141 tccagcaaat cgttcaaaat tatcagccaa tcatcgaaaa gaaacggttg acgattcgat
     1201 tgattggaaa gaacgaacca tttactacta aaacagatat cctttatcca attatcaaaa
     1261 atctgattga aaatgctgtc caatattcaa aaagcgacag cgagattatt atccgctatc
     1321 aggcaacaga tgatctatcg ttttctgtcc aagactttgg tataggtatc gacattgagg
     1381 atcaggaacg gatctttgag cgtttttacc gcgtagacaa ggcgcgaagc cgacattcag
     1441 gcgggaccgg attagggtta gcgattgtaa aagactacgt gcaactcctt aatggtacga
     1501 ttactgtaga cagtcatctt ggaacaggtt cgactttcac cgttaccatt ccgaaaatgt
     1561 aaaaaagagc aactgctcgt gcgttttaaa gttggatgac tcatcctgct tcgaaagata
     1621 cacgagtggt tgttcttttt tactcttttt tgagatactt ttcgtcaatt ttctcgttta
     1681 aatatcgata gaaatttttt agctacgcga ctaaacggct tttgctcttt ttcattttta
     1741 cgttcaaaca aatattctac caatgtggtc aaatgcctaa aaatcgaatc tgccgagaaa
     1801 aatagaatgc tcaacagcaa aagtgataca gctgagatac cggtgattga aaacagctta
     1861 tgcaaaaaga tgaccgaaaa aaagttaccg ctagcacaga aaagaatgat ctgacttctg
     1921 aatttattca gcggtgaaca gattttatag agaacgagga accctactgc aatcaaaagc
     1981 atggtagatg ctgtagaaac atctgtttta tttagattca aagtcacccc gcagatcacc
     2041 aacgcaccga ctacgatcat atctgttagt ccacctggca cagctttttc catcacattc
     2101 atgatgaatt tccctctgat tcgctttttg ttttcttcta gagcaagcaa gaaagatgga
     2161 agtccgatag taaacaaact aatcaatgtg atttgtgatg gttctaaagg ataggtcaga
     2221 gcaaagatca ctgaaaacag tgagagcaaa aaggagaaaa tattcttcac taaaaacaag
     2281 ctggctgatc gctcaatatt gttcacaacg cgccgtcctt ctgcaacaac ttctggcatg
     2341 cgtgaaaagt cagaatcaag taagaccact tgtgaggcct gcatggcggc ttcatttccg
     2401 gaagccatcg ctatactgca gtctgcttct ttcatggcta gaatatcatt cacgccatca
     2461 cctgtcatag ctactgtatt gttacgcttt atcaattgaa cgaagatttt cttttgttct
     2521 ggcttgacgc gtccaaaaac tgtatatttt tcgacagctg cttcatattc ttcttctgat
     2581 aatttagatg catcaacgta ctgatcagca tgtgaaatcc ctgcttggac agctacattg
     2641 gatacggtga gcggattatc tccagaaatc actttgatgg ctacttgctg ttttttgaag
     2701 taatcaaatg tggctcttgc ttcttttctg ataggattag caatcagaat atagcctaat
     2761 ggtgtgactg cttctgtcaa tactggttct tcaagaatcc catcgtacac accaaagacc
     2821 aacacgcgat agcctttttc agcgaaagaa acaaactctt ccgcatattc cggcaattga
     2881 tcgcgcaaaa ccatttcggg cgctcctaaa acatacacct tgtctgccaa cgctacactg
     2941 ctgaattttt ggacagaaga aaaaggaata acatggtgat aggattgatc agtggttttt
     3001 gtaaaatatg ctttaacagc ttccattgtt gcattgtctc ctgccatcgc ttttgcatag
     3061 tcagcaatgc atgcttccaa ttgatctgac tgactgatta tttcttgttt tttagagacg
     3121 atcatttttt gaacagacat tttattttct gtgatcgttc ctgttttatc tacacaaagg
     3181 acatttaccc ttgccaatgt ctcaatgctt ttcatattgt gtaaaagaac tctttgcttt
     3241 gctagtttag tcgcacttaa tgctaatgca atcgtcgtca gcaagtacag tccttcaggt
     3301 atcataccga tcaatgctgc ttccattgca acgacacttt ctttgatcgt gtttccatta
     3361 aaaaagtaac tttgagaaaa aagcaggata ccgataggaa taatgatgat cccaatccat
     3421 ttgatcaatt gattaagaga agcaatcatc tccgactgtt ctccttctcc cattgtcttg
     3481 gctttgactg tcagctggga aatatacgaa tctgctccaa ctttgtctaa tcgggcgtaa
     3541 gcttttccgg aaacaatgaa actgccggac attaatggat cacctatttc tttggttatt
     3601 tcatctgctt cgcctgtcaa taaagcctca ttaacttgca gtttcccttc cacgacctct
     3661 gcatctgctg gaatttgata tccagtctct aaaataacaa tgtcatctat tacaaggtct
     3721 tctatatcta ttgtttgttc tatcccgtct cgtatgacat tgacttttgc tgtatttaaa
     3781 acatttagtt cgtccaagat ctttttggat cgaatttcct gaaaaatggc gattcctgta
     3841 ttggctatga taactggtaa aaaagtcaag ttcttatagg attctactaa acaaagcaaa
     3901 atggctaaga taagaaagat caaattgaaa tacgtaaata tgttttccaa tacgatttgc
     3961 tgatttgttt tgaatgtggg atcgactgaa ttatttgctg cacctttttc tattcgttcc
     4021 ttgacttgat ctgccgttaa tccttcttta tacgatggaa aaagacggat gactttatct
     4081 gatgatcttt gtgttacttt ctttttttta cttcgattta tccatttatt cttcatgtgg
     4141 ctacctcgat tgatttcgtt ttctggatat ttccaaactt ctcctatcca gtatagaaca
     4201 gatatggaaa gtgttcctca cattaagctt gcaaacacct ttcattcctg tcatatagca
     4261 aaaaaaaagg gttctataat attttgtgtt ggtggaaaat cgaaggcgat tttctactaa
     4321 cactttttct tcgtttcaac tcaaaaagag actcactttt ggtaaaattt agtcgaccaa
     4381 aacgtcatcc taccaaagga gtctctcaat ggataatcat actagaaaat tactcggatt
     4441 aacagataaa catttatttt ttgatgaaga atggctgatt gaaaaagaat ataaaggcgt
     4501 tcaagcgcag tttattaaag gaaaattgga tgactcttct tcctattgtg aacactgtcg
     4561 atcggttcgt attatccgca acggttccta tacgacacgc actcagatgt tgaaagtcaa
     4621 agaaaagtta actattttag aattaaaacg tacccgtttt ctctgccatg attgtggaag
     4681 gaccttttct gcgaaaacgg acttagttga tgaacatcat caactaacaa aggaactgaa
     4741 gcaggcgatt ctcatggaac tgtacgaaaa tcaatcacgt aaactaatcg ctaagaagta
     4801 cttcgtctcg gatgggacgg tgacacggat cttgcgtgaa gcaacgaagc attaccagcc
     4861 acgtatgaac ttccttccga cggtgttgtg tatggatgag ttcaaatcaa tgaattctgt
     4921 ttctggttcc atgagtttta tttgtgttga tgggacaacc aatcagctat ttacgatcct
     4981 tgaagatcgc agactttaca aactaactca atacttcctg cgctttccta gaaaggcccg
     5041 tttaaaggtc aagtacctag tgatggacat gaatgccagc tattgtcagt tgcttaaaac
     5101 agtctttcca aatgcggaga tcgtaactga ccgctttcat attgtccaac acatcaatcg
     5161 cagcttcaat caattacgtg tccagatcat gaatcgtttt cgaacctcac actctgaaaa
     5221 tcagaagaag taccgtcggc tcaagcgata ttggaaactc ttactcaaag attccacgac
     5281 atgggagcca ctcaaacggc attatcatcg actgtttaaa cgaccaatta gtcaaacaga
     5341 aatcgttgat gagctgttaa gctacaacga agaattgcga actgcgtatc atttttgtca
     5401 attattacgc tattatttcg ttaaacgagg tacggaaggc tttcaagaaa cccttaaaac
     5461 aatttcatct acgttacctc agtcgtttaa aaagaaattc acgatcttcc attgctacca
     5521 atcgggtatt tcaaatgcct ttaaggtgtc ccactcaaac ggcgtcactg aggggctgaa
     5581 taacaagatc aagctgatta aacgaattgc gtttggttat cgcaactttt ataactttcg
     5641 ggcacggatc tatttacaac aaggtctgat ctttgaaaac taaaaaaata tgcggcgcac
     5701 agaacgcgcc gcatatcgga ctaagtttta ttcaaatttg tcttcaccaa cacgatttga
     5761 cgaagagcca aaaaaagtta cgacagttct atgtcgtagc tctttttttc g
//
LOCUS       DACIRY010000148         5809 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.154, whole genome shotgun sequence.
ACCESSION   DACIRY010000148 DACIRY010000000
VERSION     DACIRY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.154"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1158
                     /locus_tag="IYE22_002451"
     CDS             <1..1158
                     /locus_tag="IYE22_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ9507637.1"
                     /translation="GYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYC
                     EKISISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYG
                     RRTLPFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWK
                     AGDSYVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEEYVVQ
                     LLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIY
                     YLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTA
                     ITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(1231..1878)
                     /locus_tag="IYE22_002452"
     CDS             complement(1231..1878)
                     /locus_tag="IYE22_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9507638.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1886..2572)
                     /locus_tag="IYE22_002453"
     CDS             complement(1886..2572)
                     /locus_tag="IYE22_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9507639.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2578..3651)
                     /locus_tag="IYE22_002454"
     CDS             complement(2578..3651)
                     /locus_tag="IYE22_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ9507640.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3873..4547
                     /locus_tag="IYE22_002455"
     CDS             3873..4547
                     /locus_tag="IYE22_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9507641.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4552..>5809
                     /locus_tag="IYE22_002456"
     CDS             4552..>5809
                     /locus_tag="IYE22_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9507642.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQ"
BASE COUNT         1868 a         1044 c         1031 g         1866 t
ORIGIN      
        1 gggtatcgtc ttgaacattt gaatgtacca gcttatttaa atgttctgat ggaaatgtcg
       61 caaagttcta caaacagtcg tttattaaat gagattattg tcaatccggg taaaacgatt
      121 gagtattatt gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa
      181 tgcaatcaaa ttttagaaag ctatttatta aaagtgatcg ttgaccaagg ttttcagtta
      241 actagcctta aaagtgagct ccagttgcgt atctttgtta cctttttctt tttaagttac
      301 tacggaagac gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga aattatggtc
      361 aagaataatt gtcagatgaa tttgtttcag gaagaaagcc catatgagca atctttcttt
      421 attatgtatt ttgttgtgca tctcatgaga gaatcccaag gaactccatt tgaatggaaa
      481 gctggagact cttatgtaca gttgataaat atcgataaaa aagacttctt aatcttgaaa
      541 aatctcttag gaaaattgaa gtacagcagc tatataaaag tagtgaacta ttttgtcaat
      601 acgatttttt cagaattttc ggagaaagaa aaaaacgaag aatatgttgt tcaattatta
      661 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
      721 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
      781 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
      841 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
      901 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
      961 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
     1021 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
     1081 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
     1141 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
     1201 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
     1261 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
     1321 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
     1381 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
     1441 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
     1501 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
     1561 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
     1621 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
     1681 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
     1741 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
     1801 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
     1861 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
     1921 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
     1981 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
     2041 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
     2101 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
     2161 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
     2221 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
     2281 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
     2341 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
     2401 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
     2461 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
     2521 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
     2581 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
     2641 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
     2701 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
     2761 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
     2821 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
     2881 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
     2941 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
     3001 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
     3061 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
     3121 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
     3181 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
     3241 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
     3301 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
     3361 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
     3421 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
     3481 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
     3541 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
     3601 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
     3661 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
     3721 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
     3781 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
     3841 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
     3901 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
     3961 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
     4021 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
     4081 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
     4141 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
     4201 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
     4261 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
     4321 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
     4381 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
     4441 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
     4501 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
     4561 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
     4621 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
     4681 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
     4741 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
     4801 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
     4861 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
     4921 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
     4981 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
     5041 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
     5101 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
     5161 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
     5221 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
     5281 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
     5341 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
     5401 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
     5461 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
     5521 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
     5581 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
     5641 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
     5701 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
     5761 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagc
//
LOCUS       DACIRY010000149         5744 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.155, whole genome shotgun sequence.
ACCESSION   DACIRY010000149 DACIRY010000000
VERSION     DACIRY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.155"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            872..1840
                     /locus_tag="IYE22_002457"
     CDS             872..1840
                     /locus_tag="IYE22_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ9507643.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1857..2381
                     /locus_tag="IYE22_002458"
     CDS             1857..2381
                     /locus_tag="IYE22_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ9507644.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2757..3038
                     /locus_tag="IYE22_002459"
     CDS             2757..3038
                     /locus_tag="IYE22_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507645.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3040..3456
                     /locus_tag="IYE22_002460"
     CDS             3040..3456
                     /locus_tag="IYE22_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507646.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4086..5240
                     /locus_tag="IYE22_002461"
     CDS             4086..5240
                     /locus_tag="IYE22_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ9507647.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            5234..5656
                     /locus_tag="IYE22_002462"
     CDS             5234..5656
                     /locus_tag="IYE22_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507648.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT         2204 a          985 c          905 g         1650 t
ORIGIN      
        1 ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata tatgctaata
       61 atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag ctgaagcagt
      121 ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca cacgagcaaa
      181 tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag tatataaata
      241 tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag acaactttag
      301 ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat aagggattta
      361 tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa gagttcctta
      421 tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag agcagagcga
      481 tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa tgcgcgaatc
      541 tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct cttttataat
      601 ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac tctcccaagg
      661 gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc
      721 tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga attagtacat
      781 cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa cgtgtatctc
      841 ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa agtatcatca
      901 tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt tgttcttttc
      961 tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat tcgagcaatt
     1021 aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg atttagaaac
     1081 aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg gtttgcaacg
     1141 tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata ttccttttgc
     1201 tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag aatgggtgcg
     1261 ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa tgtttcgatt
     1321 actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact ttcatgaatt
     1381 acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt tcaatccaat
     1441 acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat acggaaaagg
     1501 acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa aaaaatcaga
     1561 cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag aactagctaa
     1621 ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca aagaacaaaa
     1681 gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa atacccaaaa
     1741 taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag aactaaaaaa
     1801 agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta gattttatga
     1861 cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca gcaattaaca
     1921 aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac aagtttgtga
     1981 ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac gaaaactcaa
     2041 atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc aaccaagttc
     2101 ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat atgcaactac
     2161 aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa caacaactca
     2221 ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg acttatacgg
     2281 aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg atagaaaaac
     2341 aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagcaa tttaaattaa
     2401 taaaaattgc tggttttatt cttctttttg cttttgcgag cagagcaaca tggacccatg
     2461 ttgcgatatt ttggtggcgc cccaatcccc gttttttcgt tgaatcttac tcggactgca
     2521 ctcgttttac agctgatttt tctgaatttt gaaagagcat aaggaaacgt ggtatcactt
     2581 tcttacgaaa ggtgctagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgctat
     2641 cacttttttg ccattgtggc taggctatgt atagtgtttt ggcgaaaaat agctatacaa
     2701 atactatatt tgtatggtat acataaatta tacgctgata cgaaaggaaa atttccatgg
     2761 acattttagt tcgaaatatc aatcccaaaa ttattgcaag aatagataaa cgttgtcagg
     2821 aactcacaac caaaacgggc aaaaaatgga gtcgaaataa atatctaaaa gttctaatcg
     2881 aaagtgattt tgaccgtcaa cttatgaatt acaaaaaaga tcaattcgat ttattactcg
     2941 aaaaatttat tgccattcaa acagaaaata cgaacatact gaatgactat gttcacacaa
     3001 acaaccaact cattgaaaca cttatcggag gaatataaca tgagaaaaac ttctgttcaa
     3061 aaacacgtaa ccctttatcc cagtcacgtt gacgttttag attccatcat cgaaaaaaat
     3121 ccagacgtca acaattattc agaagccatt cgttttttat gtatgagtta tgaacctgat
     3181 accaatacaa ccacaaccag taaccaagca aaactcaatg ccatgggaaa agaaatttct
     3241 atcatgacag aaatcatggc agaatttgct gatttatatc taaaagatac tggtgttctt
     3301 atcggtacaa attctaaagt gtatcaagaa gtgaaagaaa aaattgaaaa tcaactcaat
     3361 ccttcccgac gaaatcaacc caaaaatcgt caaaaagaaa ccaatcaaac ctttacttct
     3421 ggaaattttg acccaacaaa ctttctaaaa agataaaaaa agaaaccgat ccctaaaaaa
     3481 attcctcgat ttctttttag ggatcagtct tccagcaaga tttgcaagtt ggtaccaact
     3541 tgcgaaaaaa aagaaaaatc cgcttatgca acttcttctt tttgtatttt ggggcgctac
     3601 cccaatcccc aagtatcggt tatttaatag cgcggaaatc caaagaatag gacgtaccct
     3661 gaaacaaaaa aagactgctc tcacgagcag cccaaaatga gattatccta ttcatcaaca
     3721 taaaaatatt acaaagtgaa gaactagaga aagacaaaaa aagcatatca aatcctctca
     3781 aaaataacaa gtctttaaaa aatgacagat aaaaatgcac aaagaactaa aaaagagaaa
     3841 tcgcgtaaaa tcaagcatta gaagctagta actagctaga ccaattaaaa atcacaaagt
     3901 gagcgtctct atccattggt agaggggcta cttggtctag tcgacattat gttaactagc
     3961 tcgataaata acgattatgt tcccaaaaca atattttatg aactataaca aaaatttgtt
     4021 atcgcatatt gtatatttta attatataat tataggtgta taccacttaa atttaacaca
     4081 aagacgtgat tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga
     4141 tgattggaat ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca
     4201 atatcaactc ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac
     4261 actccataac ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc
     4321 ttggttcgcc atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc
     4381 cagtattttc agacgctgat aatacgatta aatatattta taccgtcaaa aaaaatacaa
     4441 atggagaata ttcaggaagt atctcacaat ttttagcaaa agaacttcaa aatctagtag
     4501 atagtaatat aacagataca accttatttt ttcaagataa tagcatttat tattccgata
     4561 atggacaaat taaactactt tggaaatcac ctttaagtac agaaaaaaat ggtgtttctt
     4621 cctttacaga aaaaaatgat cttatatccg taaatataaa aaataaaatt aaaaatttct
     4681 ctaaaaaaca aaattttcct gcggttaaac gtgtaaaaag tgtctcctcc ggagataact
     4741 ttattatgat taattggcgt atagcagaaa cccaaacaag ttttccgtgg tgtgctgctt
     4801 acgtagaagc tgctatctta tgtaacaaaa cagatgtacg tgctacttca gctagagcca
     4861 ttatgcatta tacctatcct aatttaaaca acagtcaaat tgaatcaaaa agtcttaatc
     4921 aagatcaaat aataaaatat gcaaattact gtggtgttta tcctaaaaaa gtagcacgaa
     4981 tactaaacct ttcagaggtc caaacacaac taagaaaagg aaatgccatt tatatgggaa
     5041 ctgctggtta tggaaattat agtaacagcc gtcatgcact tgcattattt gggtgggtca
     5101 atcaaggtgg ttatcaaact tactatgtgt ggaatccttg gtataactac ccaactgtaa
     5161 taagtgcaaa tgcaggacca gttacaattc ctgtacctgg aggtggtttt acttgggaca
     5221 ataccattac aaattggtaa ataaacccat tgctttagtt atgggtctag tgttagttct
     5281 tttcttaact agttgttcaa aaaaacaaga aatacaaaag actgatctga ttccttttct
     5341 aggaacttgg caaactaaag atcattcaga acgtctagta atcactaatt taaatacttc
     5401 aaaaaatata gaacaatata ccatcttagt aaaagagaac aatcataaaa ttcaaatcat
     5461 tgataaaaat ttaaatataa atgaacaagt tttcatttac aaagcaacaa ataatttact
     5521 aacactgcgc tctaataaaa aacaattaga ttacactttt agtttggatt ctccaaaaaa
     5581 gctcgtattt attttgacaa cgaatgatgg gacagaaggg acagctaaac caattgaatt
     5641 ttttaaagct gattgatgaa tagttgtttg atattacaaa acaataattt caccgaaggt
     5701 gaaacaagcg aaactttaca aaaagtggct agcatttgct agcc
//
LOCUS       DACIRY010000150         5727 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.156, whole genome shotgun sequence.
ACCESSION   DACIRY010000150 DACIRY010000000
VERSION     DACIRY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.156"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            196..921
                     /locus_tag="IYE22_002463"
     CDS             196..921
                     /locus_tag="IYE22_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507649.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IYE22_002464"
     CDS             911..1420
                     /locus_tag="IYE22_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507650.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IYE22_002465"
     CDS             complement(1490..2938)
                     /locus_tag="IYE22_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9507651.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IYE22_002466"
     CDS             complement(2938..3654)
                     /locus_tag="IYE22_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9507652.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IYE22_002467"
     CDS             complement(3635..3985)
                     /locus_tag="IYE22_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ9507653.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IYE22_002468"
     CDS             4129..4902
                     /locus_tag="IYE22_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9507654.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IYE22_002469"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IYE22_002469"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DACIRY010000151         5704 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.157, whole genome shotgun sequence.
ACCESSION   DACIRY010000151 DACIRY010000000
VERSION     DACIRY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.157"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..701
                     /locus_tag="IYE22_002470"
     CDS             <1..701
                     /locus_tag="IYE22_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ9507655.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
     gene            714..1172
                     /locus_tag="IYE22_002471"
     CDS             714..1172
                     /locus_tag="IYE22_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ9507656.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            1464..2765
                     /locus_tag="IYE22_002472"
     CDS             1464..2765
                     /locus_tag="IYE22_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9507657.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            2792..3052
                     /locus_tag="IYE22_002473"
     CDS             2792..3052
                     /locus_tag="IYE22_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ9507658.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(4051..5286)
                     /locus_tag="IYE22_002474"
     CDS             complement(4051..5286)
                     /locus_tag="IYE22_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ9507659.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1774 a          876 c         1158 g         1896 t
ORIGIN      
        1 ataaagaatt gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa
       61 tgcaagtcgc cgattttaat ataggagata aaacttggca aaaaaccttt caaatgatgt
      121 cagtcatcac gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat
      181 atcctgatat ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg
      241 atggacgaga gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag
      301 aagtattgaa ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct
      361 tgttgaatga tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga
      421 atctacggtc agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggagc
      481 caattacact aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg
      541 cagtagagaa gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag
      601 catttacatt gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa
      661 tgatcaaagt aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt
      721 tctttaaaac aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag
      781 cagtaaaaga cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc
      841 atgatggata tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac
      901 atgccataac gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata
      961 cagttgaact cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag
     1021 aagggacact gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg
     1081 tgatcattgt agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag
     1141 taaagattaa agatagttgt tttgaatttt agtgtacgat gaaaaaagtt aaatagggtg
     1201 atatttatgg gtgaatttac aaaatttttt tgaaatgata tgtttcaatc actagattta
     1261 atcattttga tttttgttaa ttatcgaatt tttttgttgc tcctatgatt tagaaaactt
     1321 attttcagga ttcttaaaaa taaatttgat tatttaagag aatatattga aataaaattt
     1381 cttaattata taattagtta tgaacattct ttgcgttgtc atgcaatgaa tggttcatat
     1441 tttattgaaa aggagtggtt ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg
     1501 tcccaccaat ggcaaaaatt ggtacacagc gtcatttatt agcaattcgt aatggggttg
     1561 tttcaacatt gtcattgatt ttgattggga cattttttat ggtctttatc aatttaccgt
     1621 ttcctggatg gaatgaattt attgcccctt attcggcaac gattgtttta ccctttagga
     1681 tgacgatggg attgatggct atttatgcaa cttttgttat gggttcggat ctagctaaat
     1741 catatggatt agatagtgtg acaggtggta tattatcttt aggaacgttc tttatgttac
     1801 aagttcctgt aaacgtttta actcctgaag aggcacctct tggctgggtc ttacctatga
     1861 gttctttggg agcatctggg atgtttgcgg ggatcttatc tatgattttt gcagtggaag
     1921 tatatcgttt ctttaaaaat cgaaatatta ctatcaaaat gccagaacaa gtgccacctg
     1981 ctgttgcccg tagttttgag gcattgattc caggtgcagt cattttaaca attacttggc
     2041 tgattcgaag cgtcgcgggc tttgatgtaa acgctgcctt attatcgtta ttcgagcctt
     2101 taaccaatat tttagggaat aatttgcttg gtgttttact accaatgttt ttgatccatt
     2161 tgttgtggtc atttggtatc catggcatga gtattgtagg ggctgtcgtt cgtccaatgt
     2221 ggctgatcat gttggatgag aatgcaaagg cattggcaga tggtacagct gccacccaat
     2281 taccctatat cgctcctgaa cagttttatc aatggacggt aacaattggt ggtgcaggag
     2341 caacgatcgt ggtatctgtt ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg
     2401 ttggtcgttt ttctattatt cctggtatct tcaatatcaa tgagcccata atttttggag
     2461 ctcctatgat gttgaatcct tacatgttca tcccgttcaa cttagttcct ttagttttga
     2521 ctattatcag ttattttgca gtcaaattag aaatggtcaa tagatttact gttttgccaa
     2581 catggacatt accagctcca atcggcggtt atctttcagc aggaaatgac tggcgtgttg
     2641 ttatcttgat catgatcaac acattgatag catttattat ctattatcca tttgtaaaag
     2701 cttatgataa aaagatgctg tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg
     2761 tctaagtttg gtttttagaa ggtgggaaac aatgtattgg ctgattttat tttttgtttt
     2821 tatcttttta ttaacggcta gtcaccttat attgaatatg ctagctacat atcatattca
     2881 aatcaatcga tggatttggg cactggcatc atttttgatc gtgattcttc caaagatcat
     2941 tgtaacgcat ttgaatgttt tgttttcatg ggggacatat gtactttgtg gaatttttgc
     3001 tatcaatttt atgatcgagc agcatagatg gtttgtcaca tcgaagcttt aaatgttcta
     3061 aaagtggttg taacagaagt ggttagcttc aagaaataag aagaaattta cgaaaattgc
     3121 ttcttaaatt tttgtgaaat gttggcttat ttttcgtttg gttgaactta ttaccgcaga
     3181 tgcaagcttt tgaccaccgt ttattcggaa tatgggactg tgacaaggct tttgtcacag
     3241 ccctttttta cataaagatt attattgttt taactgtttt ggcagaaaga aggaggaaaa
     3301 aatggaaatt ttatcagcaa tggatttaga attagtagat ctactaccaa agtgatcaag
     3361 tcaaatatag atttgggagc ttacaaccat atagtagggg ctgcagtatg gtgtatgaat
     3421 ggtaatatgt atgatgtgag tgtttattca aatcatgaaa cgtgtataga aataattgtg
     3481 ctgggaatag caatcagtca tggtgaacgt gacttttagt gtatcgttgc tgacgacata
     3541 gaacattctg acatgatcta ctcaatatgt ggggattgct ggcagtttat ataggagtat
     3601 gcatcggatt gcgagatcat tgtctcaaca gaagagggag gttgattatc aaagataaag
     3661 tggtaaatcc tacatttgat ttagtaattg ttgaggggac tagcggcaca aatctctaca
     3721 aagcgatgct tctagatcta tatgacacat tttaagaaaa tgtatttgcc taattattat
     3781 ggtctttctt ttgaagaaac gttgagatga tccaaccaga gaaaggtgtc aaagaatttg
     3841 actaagaata aatggcagac gctacctatg ctcatttgga tagcgtcaga tcttttattt
     3901 taaggatgat tttgtgtttt ttagacaccg taatttaagt aactctaact tttcaatcca
     3961 ctatttactt tttttgtatt ttcactgtct gggtctcgaa tagcgaaatt actgatgagt
     4021 ctctattaaa aaagcttttt ctacttgaaa ctatattggg tggttgctca ggaaggtttc
     4081 gatttcttga attgcttgtt gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc
     4141 ctgctgacga atcttgggtg aagaaacttc gcgcagagca atctgcagtt ttctttcagt
     4201 cactggtttt gtgagggcga ttgcgtttcc gtattttaca caatagtcaa tattacagct
     4261 ttgctcaaat tgcatcccca caccgataaa cggtttgccg gattcacagg cagtttggac
     4321 ggtcccttcc ccgccgtgga taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga
     4381 cagatattct gttattaaaa gatcttctga gtcagattga atgtcatcaa gcagatagtc
     4441 aataggtgca ataaccgcta gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa
     4501 catacgcgga ttgcctgaac tacccattgc gaaatagata atacgttttc tttcttgttg
     4561 ctttcgctgt ataaaagtta gcacttcttc aggcaaggaa gtactgagct tagcaaaaat
     4621 tggaccaacg attcgataat tttccggcag ctgttcttct tccaaaaata gtttaggcgt
     4681 tgtgatgaga tttagatcag catccatcat ttgtaaactt gtttttggta aaggaagcat
     4741 ttctttttgt gcagcgtgtt tgaaggcatt ggggatccat cgggtattta ctatacgctt
     4801 ttttgtccag tgccaaaggg gattgcttaa agggccgaag aaacgtaact cgtggggcat
     4861 cagcgcatgc tctgattgaa aaaaaggttc actcaaagca tagggtttaa catatactaa
     4921 aggaatcttt ttattgcggg ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag
     4981 tcgaggctca aacttagcaa tcagactctg ttcactagtg atacgagagg caaccatttc
     5041 tttggtaaaa gggtttttaa gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg
     5101 ttttttcgta aggatcggcg ctagtaaatg atactcaaag ccactttctg tgatcaaagg
     5161 agcaaaagtt ttagaaaagc caaagaatac acagtaataa cggtctttca tagctttggc
     5221 aacctcaatc attcgtgttg tctcagctaa attgaatact gcaggagcaa aaagaattgt
     5281 cttcatgaaa tcctccttat tggaatgagt ctatcccttc gtttcttact agtatactaa
     5341 aaaacacagc gttcgagtag cctatgtata ttttgaatat taaactgtct ctcgctgttt
     5401 tcacaacttc taaatttaaa ttttgtaatc aagttgaaac tccaattttc aaaaaaacac
     5461 gcaacaattt tttgatcgat ttatatgttg tgccagataa atacgagaag ttgtgtcatt
     5521 aaaaataaac atgagaacat aaaaaaataa aataattata ttaattgata ggtgtgctgt
     5581 cgtcattata cacacgtaag aaagaaatag ttatgtttaa aaatattctt agactttctc
     5641 gccatgacaa ttttcactat gtgttataac tgtaaacgat aattattcag ataacacaaa
     5701 aaga
//
LOCUS       DACIRY010000152         5665 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.158, whole genome shotgun sequence.
ACCESSION   DACIRY010000152 DACIRY010000000
VERSION     DACIRY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.158"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(72..542)
                     /locus_tag="IYE22_002475"
     CDS             complement(72..542)
                     /locus_tag="IYE22_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ9507660.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IYE22_002476"
     CDS             872..2512
                     /locus_tag="IYE22_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ9507661.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IYE22_002477"
     CDS             2666..3988
                     /locus_tag="IYE22_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ9507662.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="IYE22_002478"
     CDS             complement(4056..4490)
                     /locus_tag="IYE22_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507663.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5391)
                     /locus_tag="IYE22_002479"
     CDS             complement(4555..5391)
                     /locus_tag="IYE22_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ9507664.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1696 a         1128 c         1038 g         1803 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     4981 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5041 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5101 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5161 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5221 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5281 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5341 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5401 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5461 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5521 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5581 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5641 agaaagaggt cgtgagcctg ccgtt
//
LOCUS       DACIRY010000153         5530 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.159, whole genome shotgun sequence.
ACCESSION   DACIRY010000153 DACIRY010000000
VERSION     DACIRY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.159"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IYE22_002480"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IYE22_002480"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAQ9507665.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IYE22_002481"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IYE22_002481"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAQ9507666.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IYE22_002482"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IYE22_002482"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAQ9507667.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IYE22_002483"
     CDS             complement(4028..5044)
                     /locus_tag="IYE22_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ9507668.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="IYE22_002484"
     CDS             complement(5146..5343)
                     /locus_tag="IYE22_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507669.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
BASE COUNT         1452 a         1370 c          970 g         1738 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata
//
LOCUS       DACIRY010000154         5450 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.160, whole genome shotgun sequence.
ACCESSION   DACIRY010000154 DACIRY010000000
VERSION     DACIRY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.160"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1634
                     /gene="hflB"
                     /locus_tag="IYE22_002485"
     CDS             <1..1634
                     /gene="hflB"
                     /locus_tag="IYE22_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ9507670.1"
                     /translation="GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVV
                     EFLKDPRRFVELGARIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFV
                     GVGASRVRDLFETAKKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDG
                     FDGNEGVIVIAATNRSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMAD
                     DVDLKVVAQQTPGFAGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKD
                     RVISKKEREMVAYHEAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMT
                     KEDMFEQIVGLLGGRTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQY
                     EGNHQVFVGRDYGQTKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEK
                     LLEFETLDAKAIKSLFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEK
                     RDFEEAKHELNEENKAEHDSDSQQSEDNHSDDQYK"
     gene            1851..2738
                     /gene="hslO"
                     /locus_tag="IYE22_002486"
     CDS             1851..2738
                     /gene="hslO"
                     /locus_tag="IYE22_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ9507671.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            2739..3743
                     /gene="dusB"
                     /locus_tag="IYE22_002487"
     CDS             2739..3743
                     /gene="dusB"
                     /locus_tag="IYE22_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ9507672.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            3854..5350
                     /gene="lysS"
                     /locus_tag="IYE22_002488"
     CDS             3854..5350
                     /gene="lysS"
                     /locus_tag="IYE22_002488"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ9507673.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT         1870 a          969 c         1271 g         1340 t
ORIGIN      
        1 gcggtggcgg tggcggccgt gtcatgaact ttggtaaatc aaaagccaaa gaagcagaca
       61 aaaaagctaa tcgcgtacgt ttttctgatg tagcgggtgc ggaagaagaa aaacaagaac
      121 tagttgaagt tgttgaattt ctgaaagatc ctcgccgatt tgttgaatta ggtgcccgta
      181 tcccggcagg tgtactttta gaaggacctc caggaacagg taaaacctta ttagcaaaag
      241 ctgttgccgg agaagctgga gtaccgtttt actcgatctc gggttctgat ttcgtagaaa
      301 tgtttgtcgg tgttggagca agtcgtgttc gtgatttgtt tgaaacagcg aagaaaaacg
      361 caccagctat cattttcatt gatgaaattg atgcggtagg tcgtcaacgt ggtgccggta
      421 tgggcggcgg acatgatgaa cgcgaacaaa cattgaacca attgcttgta gaaatggatg
      481 gcttcgacgg aaatgaagga gtaatcgtca ttgctgcaac caaccgttct gatgtactag
      541 acccagcgtt actacgtcca ggccgttttg accgccagat tttagttgga cgtccagatg
      601 taaaaggtcg tgaagcaatc ttgcgagtcc atgcacggaa taaaccgatg gcagatgacg
      661 ttgacttgaa agttgtggct caacagacac caggttttgc cggagcagat ttagaaaatg
      721 tcttgaatga agcagcgtta gtagctgcta gaagaaataa aaagaaaatc gatgcttctg
      781 atatcgatga agcggaagat cgcgtgattg caggtccagc taaaaaagat cgcgtaatca
      841 gcaaaaaaga acgcgaaatg gttgcttatc acgaagctgg tcatacgatt gtcggattag
      901 tattgagtcg cgctcgggtc gtccacaaag taacgatcat ccctcgcgga cgcgccggcg
      961 gatatatgat cgctttacca aaagaagatc agttcttgat gacgaaagaa gatatgtttg
     1021 aacaaatcgt aggcttactt ggtggacgta cagctgaaga aatcatcttc aatgtccaat
     1081 caactggtgc ttccaatgac tttgagcaag cgacagctct agcacgaagc atggtaacag
     1141 aatatggaat gagtgatcgt ttaggtcctg ttcaatatga aggaaaccat caagtgtttg
     1201 ttggccgcga ctatggtcaa acaaaagctt actcggaaca agtcgctttc gagattgatc
     1261 aagaagtacg aaaaatcttg atggaagctc atgataaagc tcgagaaatc atcgaagctc
     1321 atcgtgcaca gcataaactg attgctgaaa aacttttgga attcgaaaca ttggatgcca
     1381 aagcaatcaa gtcattgttt gaacacggtg tgatgcctga aggtactgag tcaaatgatt
     1441 atccaagcga aaaagaagcg aaaacttttg aagaagcaaa acgtgctttg gaagaaaaag
     1501 acgctgaaaa acaagcggaa gaaaaacgtg attttgaaga agcaaaacat gaactgaatg
     1561 aggaaaataa agcagaacat gattcagatt ctcaacaatc agaagataat cattcagacg
     1621 atcaatacaa ataaaaaaat ataggtcaat tgatctgata tgactcccga aaaccggaac
     1681 aaaaaccggt tttcgggaga tttctttatt taagttgtaa atagggtgat gtcctgtttt
     1741 ctagatgatt ctgaattatt tttaaaagaa gcattccaaa gttgaaaaaa gcagtagttg
     1801 ctagtatgat tcactatgag tttaaaaacg gaaaaaggag tacataacaa atgaaagatt
     1861 atttggttaa ggcacttgct cacgacggat tcgtccgagc atacgccgtt caagcaacaa
     1921 ataccgtagc ggaggcacaa agacgtcatg atacttggca cacctcctca gctgctttgg
     1981 gacggacatt ggtcggtagt ctgctacttg gtgcgacgct caaaggagaa gacaaactaa
     2041 ctgttcgaat ccaaggcgat ggtccagcaa caggaattgt agtagatgca aatggaaagg
     2101 gagatgtgaa aggttacatc aaaaatccgc atctcagtct tcctctaaat agtgaaggta
     2161 aaatcgatgt gcggggagca gttggaacac aaggaatgtt tactgtcaca aaagacttag
     2221 gattaaaaga acctttttct gggcaaacgc cgattgtttc tggagaaatc gctgaagatt
     2281 ttacttacta tcttgctatc tcagaacaaa tcccttcagc tgttggactg agtgtattgg
     2341 tcaatactga cgacagcatc aagacggcgg gaggattcat gatccaaatc atgccaggtg
     2401 ccgatgaaga aacaatcagc aagatcgaag aacaattaaa aacgacacca agaatctcta
     2461 ccctcttaga tgaaggacag acaccagaag aaatattgaa aggattgctt ggtacagaag
     2521 aattgaacat tttggaaaaa atgcctgttc aattcaaatg tgactgttca aaagaaaaat
     2581 ttgcagaagc aattatcgcg ttaggtgcag aagaaatcca agcaatgata gacgaagatc
     2641 acggtgcaga agcggtttgt tctttctgta ataacaaata caagtatagc gaagaagaat
     2701 tagatggatt gaagaaagaa gccttaggag ggatttaagt ggaacacaca tggaaaattg
     2761 gcaatgtgga aatccctaat cgtgtcgttg ttgcaccgat ggctggaata acaaattcag
     2821 catttcgagt cactgtcaaa gaatttggtg cgggcttagt cgtatgtgaa atgatcagtg
     2881 accaaggtat tcatttcaga aacaaaaaaa ctttagagat gctccacatt gaagaaacag
     2941 aacatccatt aagtttacag atatttggcg gaaataaaga tagtctagtt gaagctgctc
     3001 aatttgtcga agaaaacaca gctgccgata taatagatat caatatgggc tgtccagtaa
     3061 ataaagtaat caaagcagaa gcaggagcaa aatggctttt agatcctgag aaagtatacg
     3121 aaatggtcca agctgtttct tcagcagtaa atatcccagt aacaatcaag atgcgtattg
     3181 gttgggatga agagcacgtc tttgctgtag aaaatgcatt agcagcacaa tcagctggag
     3241 caagtgccat cgctatgcat ggacgtacac gggttcagat gtatgaagga aaagcggact
     3301 gggaagtatt gaaagaagtg aaaaaacatt tgacgatccc atttatgggg aacggggatg
     3361 tcaaaacgcc agaagatgca aaatggatgc ttgactatgt cggtgcagac ggtgtgatga
     3421 ttggtcgtgc agcgctgggc aatccatgga tgattcaccg cacacagcat tatctcgaaa
     3481 caggagaact gattcctgaa ccaacaccag ccgaaaaaat tgctacagct aaacttcatt
     3541 tacaacgctt agcagatctc aaaggagaaa aagtagcttg tagagaattc agaacacatg
     3601 cggcttatta tttaaaaggg attcctcgag cagcgaaagt gaaagtagca atcaatcaga
     3661 cagaaaaaca gcaagaaatc aatgacctgc tggatcaatt tgtagaaaaa gcggaaaaag
     3721 tactattgaa acaaacgttg tgatttctaa gtttttcgtg aattttcaag ccgaaattcc
     3781 aagttcggct tgcttttttt gtgtttcatt ggcattatag gaaagagatt aacatgagaa
     3841 tggaggaact tttgtgacac aggaacaaca acatcaagta gaagatttaa atgatcaaat
     3901 gcttgtacgt cgtcaaaaaa tggaacaatt gcgtgaagat ggaatcgatc catttggaaa
     3961 acgatttgaa cgcacgcaca actcagaaga attgcatgaa ctatttgatc caagaacaaa
     4021 agaagaacta gcagaaatgg gattgaccgc tagtgtagct ggtcgtatga tgactcgccg
     4081 aggaaaaggg aaaactggat ttgcgcatct acaagatcgt gaaggccaaa ttcaaattta
     4141 tgtaagaaaa gaccaagtag gtgatgaggc ctatgaagta ttcaaacatg cagaccttgg
     4201 agatttcttt ggtgtaactg gacaaatcat gaaaacagac acaggcgaag tttctatcaa
     4261 agcttcagaa atcacgattt tgtcaaaagc acttcgtcca ctgccagata aataccatgg
     4321 cttaacgaat attgaacaac gctacagaca acgctacttg gatttaatca gtaatcgcga
     4381 aagctttgac cgatttatga aacgaagcca aattatcagc gaaatccgtc gctatttaga
     4441 tggcaatggc tatattgaag tagaaacacc agtattgcac aatgaggcag gtggagctgc
     4501 cgcacgccca tttatcacgc atcataatgc actggacatt gatttatatt tgcgtattgc
     4561 gctagaactt catttaaaac gactaattgt tggcggaatg gaaaaagttt atgaaatcgg
     4621 ccgcgttttc cgtaatgaag gaattgatac tacacataat ccagaattta cgatgttaga
     4681 agcatacaca gcatatactg atttcaatga tgtaatgaat ctgacagaag gtattatccg
     4741 taacgcagct gaaaaagtac taggaacagc aaaaatcaca tatgatggac aagctgtcga
     4801 cttagaaagt gactttaaac gcatccatat ggtagatgca atcaaagaac aaactggtgt
     4861 agacttctgg caagaaatga cattggaaga agcattagct ttagccgaaa aacacaatgt
     4921 agaagtaacc gaagctatgg gtgtcggaca tgttatcaat gaattctttg aaacatttgt
     4981 tgaagaaaca ttgacacaac cgacgtttgt atacggacat ccagtcgaag tttcgccttt
     5041 agcgaagaaa aatccagaag atcctcgatt cacagatcga ttcgaactat tcatagtggg
     5101 gcgcgaattt gctaatgcct ttacagaact gaacgatcca attgatcaaa gagaaagatt
     5161 cgaagcgcaa gaaaaagaac gtgaactagg aaatgacgaa gctcacggtg tagacgaaga
     5221 cttcttggaa gccttagaat acggtatgcc gccaactggt ggtctaggca tcgggatcga
     5281 cagattagta atgttgctaa ctgatgcaca atcaattcgg gatgttttgc tattcccaac
     5341 aatgagataa tcgaataaaa gctgtctaag gaattccggc tctttgtcaa taaggactga
     5401 tgggttgtac aaaatcaaat ctgggtcaga atgaacctca ttctggctca
//
LOCUS       DACIRY010000155         5194 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.161, whole genome shotgun sequence.
ACCESSION   DACIRY010000155 DACIRY010000000
VERSION     DACIRY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.161"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            19..1137
                     /locus_tag="IYE22_002489"
     CDS             19..1137
                     /locus_tag="IYE22_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9507674.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1265..2641
                     /locus_tag="IYE22_002490"
     CDS             1265..2641
                     /locus_tag="IYE22_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ9507675.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3042..4622
                     /locus_tag="IYE22_002491"
     CDS             3042..4622
                     /locus_tag="IYE22_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ9507676.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4820..5044
                     /locus_tag="IYE22_002492"
     CDS             4820..5044
                     /locus_tag="IYE22_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ9507677.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         1747 a          844 c         1082 g         1521 t
ORIGIN      
        1 aagaaaggag gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg
       61 cttgaattat tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc
      121 gaaccgatcg ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt
      181 tactatttat tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg
      241 gctgtcgcaa ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc
      301 atcccaagtc tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa
      361 caggtagaaa gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc
      421 aataaatatt ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt
      481 ggagtatcca atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc
      541 attacagcac cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac
      601 atccaacagt ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg
      661 aataaaacat tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc
      721 ttcacatttt taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt
      781 gcgggattga caaacctaat tccatattta ggaccttatc taggactagc acctgcattt
      841 ctagtaacag tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc
      901 gtccaacagt tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc
      961 catccattaa cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt
     1021 atcttcttag gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa
     1081 atcgtcaaag aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta
     1141 gttttagtac acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg
     1201 ctatagtgcc acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc
     1261 gattatgaat gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt
     1321 tttgacattg atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa
     1381 gaatcgaatc gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat
     1441 attgcagaat ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat
     1501 tttgtctggt gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg
     1561 cggcacagcg gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc
     1621 gattgttttt ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc
     1681 aaagttcact tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct
     1741 gctttctgct tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga
     1801 ttcaaaaatg accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa
     1861 tgaagaatta gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt
     1921 catggtccca cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt
     1981 gaatgaagtt ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa
     2041 agtagtcgga atccttcata caaagaattt actaaaagct gcccataaat tcggctttga
     2101 taatttagat atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat
     2161 tgatgatctg ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga
     2221 atatggcggc gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga
     2281 aatcgatgat gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata
     2341 tatcatccaa ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca
     2401 tatgagtgat gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga
     2461 tgaaggcgaa aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga
     2521 aggttctcga atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga
     2581 acctgaagaa gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata
     2641 attggaaact tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt
     2701 ttagtcagct gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga
     2761 atccatgcta tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc
     2821 attaagtaat aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct
     2881 tattgctgat aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc
     2941 ttttgtcaca gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag
     3001 agaaaatata aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa
     3061 agcaacaagt ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa
     3121 caacgattac agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa
     3181 aaggcaaaaa aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg
     3241 ggatttctgt tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct
     3301 tggatacacc aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg
     3361 atagtgcggt catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat
     3421 tccaagttgt aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg
     3481 atggtcgtga accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt
     3541 atccgatgaa ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc
     3601 ataatcgtgt agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg
     3661 atgaagatgg actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag
     3721 tcttagaaga agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg
     3781 ctcgtggcga tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga
     3841 cttttttaga gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg
     3901 atcagatggt aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa
     3961 atatgaatcc agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg
     4021 aacgaggaat ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca
     4081 cacaatttat ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg
     4141 gtgtatatga tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag
     4201 ttgcttatga agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa
     4261 atgtcatgaa acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga
     4321 ttcagcttta taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac
     4381 aattcgaagt attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc
     4441 caatgggaca caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt
     4501 caagtcgcaa tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc
     4561 aatttgcaga acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt
     4621 aaagaagtct tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag
     4681 cctctcatat tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt
     4741 aaaatcatag aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa
     4801 agaaaggaga gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa
     4861 aaaagaagag atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga
     4921 gcaaattgca caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga
     4981 cacgcgtaga actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag
     5041 ctagaaagaa aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca
     5101 caaaaatttg aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc
     5161 tttcgccgtt tatacgtgtt tagagcgtga gaca
//
LOCUS       DACIRY010000156         5096 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.162, whole genome shotgun sequence.
ACCESSION   DACIRY010000156 DACIRY010000000
VERSION     DACIRY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.162"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1447)
                     /locus_tag="IYE22_002493"
     CDS             complement(<1..1447)
                     /locus_tag="IYE22_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ9507678.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERYPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIF"
     gene            complement(1498..2319)
                     /locus_tag="IYE22_002494"
     CDS             complement(1498..2319)
                     /locus_tag="IYE22_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9507679.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(2316..3125)
                     /locus_tag="IYE22_002495"
     CDS             complement(2316..3125)
                     /locus_tag="IYE22_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9507680.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(3146..3640)
                     /locus_tag="IYE22_002496"
     CDS             complement(3146..3640)
                     /locus_tag="IYE22_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9507681.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(3652..4140)
                     /locus_tag="IYE22_002497"
     CDS             complement(3652..4140)
                     /locus_tag="IYE22_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9507682.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(4217..>5096)
                     /locus_tag="IYE22_002498"
     CDS             complement(4217..>5096)
                     /locus_tag="IYE22_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9507683.1"
                     /translation="MGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     DYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLEHILSP
                     EMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
BASE COUNT         1569 a         1150 c          837 g         1540 t
ORIGIN      
        1 aaaagattaa cccttgctca agaatcggtt tttgatagtg cttcactgaa taggttgttt
       61 tctcctcatc accgattccc tccaaaaatt gctcaccaaa aggaccatcg atagtcattt
      121 gtttacaaat tgattccttt agtttacagg caatgttttt gtatcgcttg ctaagactca
      181 gttgttctag ggcttcaaaa atttctgcaa gtccactaca ggccttccaa agacaaagat
      241 tgctgccggt atgatacttt ccaagtgcaa aggcgtctga taaccaaaca gatggaaaaa
      301 gcattggttc ccctgaccga cgttgagcta atatgtcctc aataattctt gaagcattca
      361 gtagaacctg cggataccgc tcaaagaatt ttgtatcccc agtatgttca aaataaagac
      421 tagccagtaa cgcactggat aacgaattac tcagagaatg attgatacct cctgtaaatt
      481 ttgaaccagg aaacttaacg ccatattgat caaaccatag aaccgttttt tgacataact
      541 ccggttcaaa catggtgaat ggcaatgatg cgtaatacat atccttattc caaattcgat
      601 tagtagcagg atagctgccc caattgctgc cgacgacctg tccctgtcga ttcataccga
      661 aactagaaaa gctttgataa agtgcacgac gatagagatg gacaactcta tcgtttggca
      721 ttgacagctg cccaaatagc tgagcaaaat agttcaatgt ttccttcagc catttttcga
      781 tatcagattc ctgaaaagtc attacttctt caaaagcatt tgggtccaca aaagcaatgg
      841 cgaagcttgc cacttcctgt tgtgcgatcg tgtccttatt tcctttttga gaaatcaaca
      901 cattttgctg atccgaatac ttctcttggt acaaaggcac atctttcact gaaacttgaa
      961 ggctttcgtg tgtttcattc acaatgtaaa cctgttgaac aagcatactt aaacgcttat
     1021 tagccagtat tggagcaaag gatactacaa tgaccttaaa cgtcgaataa tgatgaatat
     1081 atctaggcaa acagtcttga atcacatcga cttcaatacg atgatccgat tctgacaaat
     1141 ggtagacaat cccgtttact tcaaccttta aggataacga attggtttga gaaagctgtt
     1201 tattgaccca aacacctggt ttttcctcag ttagtaaatt ttctcgataa aaggtcaacg
     1261 tttgaatcgt cccgcaaaca tcaaggtttc ctaatgtctt ttgattactt acatcgaagg
     1321 tcaatccctt tacatcagga atattccgat agtagctgta ttcgtttgaa gtagtcacat
     1381 ttccgtcgtt taactcagca ttaaataatg ggtgtttggg tgaataaatt acttcattaa
     1441 tagtcatctt ttccctcctt gctaaaaaaa ctgcagccta gtagctgcag cttcctacta
     1501 tgcgattcca agtaatgaca ataaaattcc caagacgacc attccaaaaa taattttgat
     1561 gacgccgact tttttatcta gtaatttgaa acagcccaat gttaccaata acggtacaat
     1621 tcctttaaaa atactatcga ggatttcttg gacattcatt agttcttcgc ccttcattga
     1681 aaagctcaaa acggtattga atttgaccat actgatcgtc attgcgccta ccatgatcag
     1741 acccaagata ctagcagctt ttgtcagaat ttcaattaac ccgttcgcat acagcttttc
     1801 aatatattta gagcctaatg aatagcctaa aacgccgcca tagtatcgca tcaaataatt
     1861 aggtatattg aacataacta aaaagataat tggtgccaaa atatttccgg cattggctaa
     1921 gttgatcgct agtcccgcgg taacgacacg ccaaactccc cagaacaatg aatcgccaat
     1981 tccagccaat ggtcccatca aactcgtttt gatcgcgttg atactttcca cgtcaaaacc
     2041 agctttttca gaattttcct tctccatcga tgccgcaatc cccatgacaa aggacgcgca
     2101 aggaatggtc gtcgaaaaat actgtgtatg acgttccagc gccgcagctc gatcttcttc
     2161 acttttgtag aaatgattaa tgaagggcat catagaatag gcgaaaccac cgccgcccat
     2221 cgtcaccgga ttcaccgaag catacatagt catcgagcgc caaaacatct tccgaaccaa
     2281 ttttttctca tctttagaaa ttccatcagc taatttcatt gaaagaaatc ctcctcttct
     2341 tggtcaagcg ttgcatttac agcagcatcg ctttgaactg catgaacaga tatatttttt
     2401 agttgtagat ctctttgggc tataacaatt gcaataatcg ttccaactgc ggcaattgca
     2461 atcaatggta actcgagata cgccataaca acggccccaa agaaaaacca cacagcaata
     2521 tttttactcc aaagcatccg taacagcata gccatcccaa ctgccggcag cagatttcca
     2581 gcgactgtta atccgttcat gatgacctct ggaatattat ccaagacgct tttgacaaca
     2641 tctgctccaa ataaaacggc aaagaatgga ataagagcat aaagcgcata gtggacaaac
     2701 cacataacga agtgcagatt gatgattcct ttttgatcac cagattttgc aaatttgaaa
     2761 aatttagcag ccatcggtcc acatacaaaa gaaaaaatca tattcattaa ctgtaatcct
     2821 aatactgcta gtggaaagac aatgggaata atcgcatcca cgtttcctcc cattttgatt
     2881 gcgaacgctg tagccagccc agcagccaaa gaaggctccg ccgtactaga gattccgata
     2941 ttgacgacac ccataaagat ggcttctagt gacgccccga caatcaaccc cgtctgcaaa
     3001 tcccccatca acgctcctgc taacggagca acaacaatcg gacgactcag catggacatc
     3061 ccgaacattt catgtccagc aactccaata aaaacggcca aagcgactac aaatgcatca
     3121 tacataattt ttctccttcc tttttctaaa aatcactcaa aatagagata gctgaacgtt
     3181 tttccatcgc cggtgtaggc tgaaaaacgg tatctggata aagttcaact aattctttta
     3241 agtcttgaat ttctgacgtc gttaacatca cttcctttga taaggttttc ttatcttccg
     3301 atggcggtgt cattttccca gcattcccca cgttcattaa ttcgatttcc gttgggaaag
     3361 ctttcgcaac ttttaccgca tctttgacat tagggactaa aaccatcaag cgcatgtcct
     3421 tcgcccgcgg atcttttaac aaatgaatcg cgtcatcaac tgttttaata atgactttga
     3481 cattcgatgg tgcagccatc ttcaatgtca tcttttgaat atcgttggta gccgcctcat
     3541 cgcttgctac tactaacccg gttaaattta gactctttgt ccaagtcatc gctacttgtc
     3601 catgaattaa tcgttcatca attcgcactg ctttaatcat ttttcttcct cctaaaaaaa
     3661 atcttcctct tcatttacta catgtacctc atcaagaatg gtcatcttta attcctgttg
     3721 gcatttattt accagttcct gaatatcctc ctttgttaat tccttggttg ccaatactat
     3781 ttcgagcaga attggcagat taaagccaga caaaatagtt acagcaatct ctttctccaa
     3841 aaaggttttg gtaatcttct gattgacact tccgccaaat aaatcggtaa aaacaaaaat
     3901 ctgatcctgc tcgttcacct ggtttacaaa tacattcaac tcctgttcaa aatcacactc
     3961 atccacataa gcattaatca ccgtaatctc ttgaaccttc cccgtcagta attgaatact
     4021 gctaagaaag cctgaagcta agtgaccgtg agtcgctatt agaattttct tcatatggtt
     4081 aattcctttc tttctgttca cttttatata agcaattcct gtgccaactt ttaaaaacat
     4141 tggtttaatc acgttaattc acattaaaaa aagtgtctaa cctaaaaaaa acagattaga
     4201 cactttttta gacactttat ttttgaatat acggtcctaa taattcatag agcagcgaaa
     4261 gctcataagc attgacttta aaattatatt tcatctccat tgattgaaaa atacttctag
     4321 aaacataata aaattcatct tcatctggtt tcaaatcata attcggttct tcagattttt
     4381 ctgtccgagc caagaataaa cgttccatca tcaatgccgt atgcatatag agatttagtt
     4441 ttacacggcc atttaaagtg aaatgatagt aattttcata tttagaaatg actgtttcca
     4501 cttcactcat cacgatcgtt gggttcagaa agttcaaacg gtccgcaatt ccttccaacg
     4561 aaaagaattt tactagctcc tgaatcaagc ggttaaattc tgccggcttc atttcaggac
     4621 tcaaaatatg ctccaaattg gctgctccat caccgtctaa gatgtcataa acattaatat
     4681 taggaattga aaaggaacgt ggaagatccg aagtcgtgat gaccaatttt gtcttgtcaa
     4741 aataagtttc atcattttgg tcgattaatt cacgtagttc tctgtagtcc ttcgtgaaaa
     4801 cttcgatgga ttcatccgat aaatgatgac gaaaaacatc tttcaatttg tccgaaatcc
     4861 ccaaacccga catacaggaa atgataatgt tgctactttg agaaaagcct tcaaagtatt
     4921 gaacattgat tgaatacttg tctgctgcat cctccgcaat ctgtttaaat ggcgcattca
     4981 tcgtcatctt caggccaata tcaagtgcaa tggaggtcgt caaattattc agaagaagta
     5041 attctccttt taaatcgttc ttgatctgtg tgtagatttg atttaaagag cccata
//
LOCUS       DACIRY010000157         4986 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.163, whole genome shotgun sequence.
ACCESSION   DACIRY010000157 DACIRY010000000
VERSION     DACIRY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.163"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IYE22_002499"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IYE22_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ9507684.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IYE22_002500"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IYE22_002500"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ9507685.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IYE22_002501"
     CDS             2591..3376
                     /locus_tag="IYE22_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9507686.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IYE22_002502"
     CDS             3389..4240
                     /locus_tag="IYE22_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ9507687.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IYE22_002503"
     CDS             complement(4364..4816)
                     /locus_tag="IYE22_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9507688.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1687 a          815 c         1038 g         1446 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaa
//
LOCUS       DACIRY010000158         4806 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.165, whole genome shotgun sequence.
ACCESSION   DACIRY010000158 DACIRY010000000
VERSION     DACIRY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.165"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(52..1200)
                     /gene="nagA"
                     /locus_tag="IYE22_002504"
     CDS             complement(52..1200)
                     /gene="nagA"
                     /locus_tag="IYE22_002504"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ9507689.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1276..2112)
                     /locus_tag="IYE22_002505"
     CDS             complement(1276..2112)
                     /locus_tag="IYE22_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9507690.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2296..3513)
                     /locus_tag="IYE22_002506"
     CDS             complement(2296..3513)
                     /locus_tag="IYE22_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ9507691.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3896..3979
                     /locus_tag="IYE22_002507"
     tRNA            3896..3979
                     /locus_tag="IYE22_002507"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3930..3932,aa:Leu,seq:gag)
     gene            complement(4219..>4806)
                     /locus_tag="IYE22_002508"
     CDS             complement(4219..>4806)
                     /locus_tag="IYE22_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507692.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1460 a         1001 c          775 g         1570 t
ORIGIN      
        1 accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg
       61 gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat
      121 aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat
      181 tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac
      241 aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag
      301 acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg
      361 catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc
      421 cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa
      481 gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg
      541 cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc
      601 ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt
      661 gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc
      721 tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt
      781 ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt
      841 agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc
      901 tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc
      961 agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg
     1021 aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac
     1081 ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata
     1141 gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat
     1201 tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag
     1261 aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc
     1321 atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa
     1381 taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac
     1441 ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa
     1501 aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct
     1561 tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat
     1621 acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta
     1681 gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc
     1741 gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga
     1801 aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact
     1861 atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac
     1921 atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag
     1981 ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat
     2041 tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat
     2101 taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac
     2161 tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt
     2221 aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa
     2281 atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc
     2341 tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt
     2401 ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac
     2461 gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa
     2521 agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata
     2581 ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc
     2641 gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga
     2701 caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat
     2761 ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac
     2821 gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg
     2881 atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc
     2941 ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat
     3001 agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc
     3061 ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca
     3121 caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat
     3181 acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt
     3241 cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt
     3301 ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt
     3361 tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc
     3421 ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc
     3481 ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa
     3541 gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa
     3601 attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa
     3661 cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa
     3721 tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta
     3781 agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca
     3841 tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga
     3901 tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt
     3961 tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg
     4021 tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta
     4081 caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa
     4141 cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag
     4201 cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag
     4261 cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc
     4321 tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat
     4381 tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat
     4441 catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac
     4501 aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa
     4561 aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat
     4621 cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata
     4681 aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca
     4741 tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc
     4801 taaaaa
//
LOCUS       DACIRY010000159         4802 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.166, whole genome shotgun sequence.
ACCESSION   DACIRY010000159 DACIRY010000000
VERSION     DACIRY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.166"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(497..838)
                     /locus_tag="IYE22_002509"
     CDS             complement(497..838)
                     /locus_tag="IYE22_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ9507693.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
     gene            complement(828..1109)
                     /locus_tag="IYE22_002510"
     CDS             complement(828..1109)
                     /locus_tag="IYE22_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ9507694.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            complement(1216..1800)
                     /locus_tag="IYE22_002511"
     CDS             complement(1216..1800)
                     /locus_tag="IYE22_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507695.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            complement(1817..2056)
                     /locus_tag="IYE22_002512"
     CDS             complement(1817..2056)
                     /locus_tag="IYE22_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507696.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(2075..2323)
                     /locus_tag="IYE22_002513"
     CDS             complement(2075..2323)
                     /locus_tag="IYE22_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507697.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2338..2904)
                     /locus_tag="IYE22_002514"
     CDS             complement(2338..2904)
                     /locus_tag="IYE22_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507698.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            complement(2905..3450)
                     /locus_tag="IYE22_002515"
     CDS             complement(2905..3450)
                     /locus_tag="IYE22_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507699.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3476..4243)
                     /locus_tag="IYE22_002516"
     CDS             complement(3476..4243)
                     /locus_tag="IYE22_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ9507700.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1350 a         1041 c          588 g         1823 t
ORIGIN      
        1 cgttcgttcg tcttttcctc cgtgggactc acccaccgca agcggttggt tgagttgtgg
       61 ctcctattca aaattcacta atatgttatt tttgttttct attcaagtgg gtaacgttcg
      121 tttgcaagca aactctctct atgtaacttg ccagttacat cagtggtaac gctacgtcgc
      181 aagcgacttg ctctaatgaa actaccgttt cattctgcct gttcgttcgt agcaagctac
      241 ttcactcact gtatccgtct gaattattgt tatttattac tattcaatat ccgtcagtca
      301 ttttatttat ttacatacat taaatataaa atgtatctta taattcgtat attactaatt
      361 atcactattt aacaatactt aatattttct taatttttat aatcacgtat atatttacgt
      421 gtattttaat atacataaat acgtttatat aaacgtatat cttaatatac atataaacaa
      481 aaaaagaaaa gccttgttag acttttcttt gtaatctctc gataaaccta tttagctttt
      541 tcgtatcaat agctgatagc ataccaacct ttttaaatga tatgttctct cggcttttgg
      601 gaatagcctt taaagaccct acgtcaatcc atgattgctt ctttagacct actttttccc
      661 attctgcaat aggaaaatac actctcttta tttggtctga tttattctca tattgacttg
      721 tgattccaaa aaattctact ctatcttctt tatcactaac caccaaaata ggtcttcttt
      781 taccaccatt tttatctgta aatgcaacat agaccgttac tatctcatta ggcttcattg
      841 aaccactctt ctaacttttc atctgtatct agaacatcaa ctggcatatc catagttagc
      901 tgttgcaatc tgattccttg cttttcctct tcgcttagct ttatctctac tggaaactca
      961 cctctagcga ccacttgctt ataaaggatt gtgattgctg ttgttggatt taaccctagc
     1021 tcttccaata tcatttctgc gttttctttt aaatccttgt ctattccgac ttgaattctg
     1081 cttttttctt ttggattttt taccttcatt tcgttcacct cttttgagta taatatactc
     1141 attattatat ccattaacta catttatgat tatattatac tcttaattag ctactttgca
     1201 aacaatccaa tcaaatcaag attctacttt actttttata cgttctttca tcttctggta
     1261 tgcaacctct actctttgga catctatatc caatccactt acaaccgtac acataccaat
     1321 ctcatcgcta tccgacactt tatcatattc ttcttcggtg atttcaatag gcatataccc
     1381 atcattaaag catatctcat catctgaata gttttcccat tcctcttcat catcttctga
     1441 ataagtgaca accctatctg ccccctgttc taccatatca tcgtaactat cagcaacata
     1501 ctgcgctttt aatcctttca gttgtaagtc atataaacca tacaacctct tactatcttg
     1561 ttcactgaat aaaattcttt gacgttctaa aattaattct ggttcatctt ccacctcttc
     1621 atctggttct gtaaaatacc attcgataaa ataatatcta atatcctcat tcgataaact
     1681 ttcaaagtcc atattctcct catcaaatcc atcaaaagcg ccactacttt gcataccatt
     1741 caaccaatta atggttctca atcttgcgta ttcacttaat accatcttat tctcattcat
     1801 ggttcttcac cccatattaa ttattttcaa attgacaaat caatttattt tgagttaaca
     1861 aaactaatag ctgttcacta tcaaaaagaa ccttacaaga ttctttttct attctctctt
     1921 ctggcatgta atagccaatc tgttttaaca tcttatctgg ataacctttt tgtttggctt
     1981 ttctcaaaag ctcttcatca ttcccaacat aataataaga acaccattgc atacctttac
     2041 ttttgaattt tgtcatgttg ctaccctctt tcgtttattg acccaaccat gaaaaaatat
     2101 attttgctaa atcttctttt tctatcccat ataaaacatt ctctaaatcc aatttatact
     2161 cttcatcaac aaggctttct gtattgtcat cataaaggac atagactgca taatttcttt
     2221 cgtaaaaggc attagcaaga cttctgctaa tacctaccat tcctttccat gtataaccaa
     2281 aatcaatcaa atcttcatga tctataaaat ccattccttc catgctgatt cacactccta
     2341 ttctgctgtt gtgtaatatg caacgcaatc atctgtccct actaaccaca cacaccaatc
     2401 ttcaatttca tcatcgcttc tcttactcaa ttcctctttg gtacaaaaaa ctacttcacc
     2461 attttctgtt tccatctcat aatcttgtag catgtattca aaccaacttc tttcggttgg
     2521 aaattctatc tcgtggcaac tctggcaaat aaaatcacca tattcattga ttcggtagca
     2581 accttcatct tctacaaatt catgattaca acaaacacat ctagtacctg ttaggacttc
     2641 cccattaatc gttgttctaa cggtatcttc tcttgctacg tgttcactgc tgacaagcca
     2701 atcaatgatt ttctttgcca attcttcatc agaatcggca aagcctttaa acgtgtttct
     2761 ctttccttga aagtagtatt tgcaacttgc ggtagtttct atggttccat aacttttttg
     2821 gaataattca atcatttcac catcaatcac tttttgactt ttcgcttcta aattagggaa
     2881 ctggtctttt cttttttcaa acacttattt cacgctctct tcttctaggt ttattccata
     2941 attttctagc aataatctgt atcccttaat aaaatctttc gtagcaaact cttttccgct
     3001 ttcacaaaac ttgtttagtc ctatctgttg accctcaaaa tcaaacaatc gcaatctggt
     3061 tttatcatct agactaaatc cattttcatc gaacaccatc tctaaatgag catagccatc
     3121 attgttatct gaaaacttca tcacatcttc aatcgtttct tgataaggtt catagcgccc
     3181 accaaaccct gtttcatcat aagcactcac tccagattca taggtgacaa aattgataac
     3241 tcctgtactt ctatcaaaga ttccttttgc taattcttct cttgatttat ctcgtaagtc
     3301 gtatgcttta ttgattaaat aaattccttg attcaatacg tccatcagca tatatcgacc
     3361 atacccccac tgataatgaa tcgacacgct tgctttcgtt ttgctgttac catatttgac
     3421 tgtaattaac gcttgtgttc tttcgcccat cttgttttcc tcttttctct aatttttata
     3481 aagaaccatt ttcttgaaat gagtaatagc ttgtgttttt acttgttacg atgatatggt
     3541 ctaacaattc aataccaatt gtttttcctg cgtttttcaa attcgtagta aaacttttgt
     3601 cattttcact tggaaattca tatccgctcg gatgattatg tgatactgcg atttttgctg
     3661 aattgtttag caaagctagt tgaaaaatct ctcttggtat cgctactgat tgattaattc
     3721 cacctttgaa aacttcgcta taagcaatga cctcattctt tgtattcaga cacatcacta
     3781 gcaaagattc actagcattg tttccaatta agttagtagc aaagtttgct agaccaaccg
     3841 agcttgtgac tctataaaaa cttctatctt cactgatata tttttgttcg attttcaaga
     3901 cattcttaat caatccttca ccttcattct cggtcttgtt ttcaacttca atagattcaa
     3961 gactgctatt tcccatatca gaacccaaca aagcatttct gaaatccaat accttttcaa
     4021 agttatcagt cataaccatt tcataggtgc tattccattc attcaaatat ttctctttga
     4081 tgagaatgaa cgctctcggt ttacttctgc caattctgat attttgtgta tcgtgaaaaa
     4141 tatagttatc aagtaaagta ccatcgtact tttcatagtt cccactgata tttgttaact
     4201 tgtttaccat ttcttgatta acttctaaaa ttctatctct catgttgaac gcttctttct
     4261 gtttttgatt tttggttttt caaaattctt ttttgtttgg attgttttag agggaggtgg
     4321 ttttttcttc atcgctattt ggttttgcat tttatgtttc ttcctttcgt tcatttattt
     4381 atctttttcg cctgtcttgc cttgtcggga gtgattagaa tgcgttctta tctgccggat
     4441 taatacaagt gtttaagtaa attattttcg taacgcttgc gtttagaaaa aaatttacgc
     4501 cacttgtatt aatccaaaga tgagataagc tctaaacact tctgacaagg tatgacaggc
     4561 gaaaaagata atgggcgtaa ggaattataa atggcaaaac caaataaaga gcgatgaatt
     4621 tcaatgcttt caggcgtagc ttgaaagcct aaaagtcttg aagaatgaaa gactttttac
     4681 cttcaaagca gacctagtaa tcttaaatag gcgatggcta tttaagatta cggtgttgac
     4741 ccactaaata aacagaaatg gaaccgatca agcaaaatcg gactaggcga tggctaggac
     4801 gg
//
LOCUS       DACIRY010000160         4746 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.167, whole genome shotgun sequence.
ACCESSION   DACIRY010000160 DACIRY010000000
VERSION     DACIRY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.167"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..797
                     /locus_tag="IYE22_002517"
     CDS             <1..797
                     /locus_tag="IYE22_002517"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ9507701.1"
                     /translation="AVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLPIAVSGADIDA
                     LMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPGMQYFSEWWKQ
                     LYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKPRKSITIPEQA
                     EDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYTLGYTMYFFEI
                     AVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(1105..1491)
                     /locus_tag="IYE22_002518"
     CDS             complement(1105..1491)
                     /locus_tag="IYE22_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ9507702.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            2370..2582
                     /locus_tag="IYE22_002519"
     CDS             2370..2582
                     /locus_tag="IYE22_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507703.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(2860..4659)
                     /locus_tag="IYE22_002520"
     CDS             complement(2860..4659)
                     /locus_tag="IYE22_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ9507704.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         1613 a          849 c          859 g         1425 t
ORIGIN      
        1 gtgcagttaa agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca
       61 tcggcggacg tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg
      121 cagatatcga tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca
      181 aactagaaga aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag
      241 gcaaagtaac tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt
      301 ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg
      361 caaacttctc aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata
      421 tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag
      481 cagaagattt agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa
      541 aagcatttga aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca
      601 aaattccaga aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg
      661 taggtatttc aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt
      721 acaagaaaaa catgttcgca cttctaggca aaccgggctt tgaagatctt gctaaagaat
      781 tgaacgaacg cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac
      841 atgttcgctt taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt
      901 attcagaata aggggctgtg acaagacgtt tgtcacagtc ccttgtttgt atagaaatcg
      961 attgattgcc ttgctcttta gtttgtttta ggactttaag aacagtgagg ctgacttcga
     1021 aggttaattc tgtttttttg ttttctagaa tcagtaagca aaaaagcact cacttcgagt
     1081 gcttttttgc gatatattct tagcttaaga taggcctaat ttttctttta cagatgaggg
     1141 taccttatcg ctggtaatta cttttttctc tatgatccgc ttttcgcttg ctttcaactg
     1201 caagatcgtt ccttcggtgt aagcagaacc attggcagag tcaccagaag taatgacatc
     1261 gaaatctaac tgtctttttt caccgttttc agcataagca tctacgttgt atttaaaacc
     1321 tcttccaatt acttccccat tatctgcttt gatatcagtt tctgcaggca ttggagcttt
     1381 gactaccgca taataatctt tacctacata agtagactga tagtagtccc aacctttcca
     1441 tcctataaaa gcaatcaata tgataacgat caaccctaaa atttttttca tgaaagatct
     1501 ccttttttat ttttattata ttaaatatta tacagataat aacggaatat tatcatcgaa
     1561 acagctaaca aaattgtcat aaaaaacgag atggttgata ttagaacttg tgtttttgtt
     1621 taggtctaga gataaagaga gacaatttcc aaatatatgt aatgaataca aaaagacgtt
     1681 tgtcgcggat taccgttaca aacgtctttt attgattttc cgagatctca tcagtcgcca
     1741 tttggaatgg ttcactcctt atctagattc gtttaatgct cggactgctt gttcgattgt
     1801 aataccaaac gcaacgttat tttcgttttg tgcatcaatc gccataaaac ggttagaagt
     1861 tgtatcaaaa gctacgcggt attgcatatt tgtttttgta aacgtcataa aattcatctc
     1921 ctttgtaatg taatttagtt cttgaaagtt tcctttaaac tttcgaactt ataccaagtt
     1981 tagcatcgta cgttttttga tgcaattaaa aagcatttat cattttcctt atttattttt
     2041 tcttaaatgg tttagctatt ttcttttaat ccgataagta gcccaatcat caaggttaca
     2101 aatactattg cttgaatata aatcatatgc tcacctcttt ataaataact tagcaatata
     2161 aagaaaaaaa cgcacgaaca acataggaat ataaacaaac gtttttgtca caacgtctaa
     2221 gatcgtgtta aaaaatagtt atctaaaaag cagaaagata ctcaaaataa aaaataaaaa
     2281 aatgaataaa aacgttttca aatacaaggt aatttgttat tataaaataa agaaaaaaca
     2341 aaataaaaat aaagaaaaga gggaatgaaa tggtaaaagc agaagtagga agatcttata
     2401 tatgcaaacc gatcggagta tctcatgaac taatcggctg tattgaacgt acatatatca
     2461 acacggctgt cgtgtatgtt gaaagttatg accaacgaga ctatcaactt atcaatgaaa
     2521 gcaagtatcg catgctcgta aaattctgtg atattagcgc acctactgaa ttggtttctt
     2581 aaacagtatt tttgagaatt cgcaagaact cccttataat ataaataaag acagaaggga
     2641 gcgagaagat atgccaaaaa gaaaaagttc agggaaaatg ggaattattc ttgcagtgat
     2701 ctctgcaatt cctgctattt tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta
     2761 agcacacatt atgagcgtca aacaaaaaac gcatatacct gcggtggcag gttatgcgtt
     2821 ttttgttgta taaagacttt tttcattaac gatttttgat tagccgactc ccaaaacaaa
     2881 atttagcagt ttatctgaat aagtgatttc ttcatctgtt tgtttcacac tttcatttag
     2941 tagtcccatc tcatcggtta tgataccatc cacaccataa aacatcatgc gactcatggc
     3001 atctgaatcg ttgattgtcc atacatatac ttcttttccg tcttggtggg cagaattaat
     3061 gaagttacgg ttgatcgtac tatattccat tgtcagaaaa tcagcaggcg tcacaggggg
     3121 cccaacaata ttgaaaggta agatgtagcc cacataaaag tttggttctg cttcttttaa
     3181 atctgaaacg gtttggtacg tcaaactttg caaaatatga tggttctcta agataacttt
     3241 gcggtattta tggacaaatc gttcgacaag gtcatccgaa tcttttttag aggtcttgat
     3301 ctcaataagt aatttttggt ttagcttatg tgctttttcc aaataagaat caaaagaaca
     3361 aacaggggct tgcactccgt tttcagtaac tgtaagattt gtcaattctt tcaatgtaag
     3421 atcctgtggt gttccattta cccctgttaa gtgacgaaga tttgcatcat gcatcactac
     3481 gaaatgacca tcttttgttt cctgaacatc catctctaca taatcaggat gatagtctct
     3541 gcttgttttt tcaagtgctt ctaaagtatt ttgcgcccca ttttgggtac tcacacctct
     3601 gtgagagaca gtcactggtt ttgtctgaac agctgaagtc agataatcca tattgtaaag
     3661 acagacgcca ataccgaaaa aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga
     3721 gaaacgcaag ttgggggctt gtgggataaa ccacttaggg atctcaggca agaagccttc
     3781 gtcatccatg aaatcaacaa tgatataaaa aatccctacg gtagacatca cgatattgaa
     3841 taataaaatc ccttgtaaaa gcgtcatagc aaacacagcg ctgatcaatg catagtctgg
     3901 gaaaaattgt tcaaccatac tttgtgccaa aataacaacg atatatccta aagaagaaag
     3961 gagtaaaatc gtaccgccaa tcacaataaa ttggcccgtg atggccaaaa gacgcgattt
     4021 ggtcaatgac cagctttccc gaatggctgc tcgaaaagaa cgatctcgta gaatcatttc
     4081 cggtaaagca aaaatcagtc gaatcccaat atagccaagg aaaatataaa cgagtaaaaa
     4141 ggaactgacg atgatccaac gattagcaaa aataaaatcc ataatgaaag cggggatttt
     4201 tatttttgac agtagatcag aattaaaact taatccgctg atcggtagaa tcaataaaaa
     4261 ataagcaagg aaaaacaaaa aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa
     4321 gtgtaagagt tgcttcaaag agattggttc tttcttttta ataaaaaaaa cactcatcaa
     4381 aagaaaagta aattcgaaat aaactaacag taaaatcgtc atcaaaacta cgattaacga
     4441 gagtagaacc ccaggatgtt gagaaaaaat tgaaggaata ttgtcataag acaaatagtc
     4501 aattgcccct cggtgtaaaa taaaacgggt cgtactgctc aataagggta aacaaataaa
     4561 taaaataaag ccatgcatca gcaagacatc acggaaataa gcttgtgttc cttttaaaaa
     4621 gtcccaagca ttacctaagc tacgtttcaa ataattcatc ctatcacctg ctcttcacat
     4681 tgtagcgcaa atctaatgaa aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag
     4741 atttaa
//
LOCUS       DACIRY010000161         4744 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.168, whole genome shotgun sequence.
ACCESSION   DACIRY010000161 DACIRY010000000
VERSION     DACIRY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.168"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            25..696
                     /locus_tag="IYE22_002521"
     CDS             25..696
                     /locus_tag="IYE22_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9507705.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IYE22_002522"
     CDS             755..1195
                     /locus_tag="IYE22_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507706.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IYE22_002523"
     CDS             1207..1875
                     /locus_tag="IYE22_002523"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ9507707.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IYE22_002524"
     CDS             1939..2562
                     /locus_tag="IYE22_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ9507708.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IYE22_002525"
     CDS             2618..3004
                     /locus_tag="IYE22_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ9507709.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IYE22_002526"
     CDS             3217..4608
                     /locus_tag="IYE22_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ9507710.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1580 a          782 c         1011 g         1371 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttg
//
LOCUS       DACIRY010000162         4716 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.169, whole genome shotgun sequence.
ACCESSION   DACIRY010000162 DACIRY010000000
VERSION     DACIRY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.169"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            17..132
                     /gene="rrf"
                     /locus_tag="IYE22_002527"
     rRNA            17..132
                     /gene="rrf"
                     /locus_tag="IYE22_002527"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(185..655)
                     /locus_tag="IYE22_002528"
     CDS             complement(185..655)
                     /locus_tag="IYE22_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ9507711.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(814..1272)
                     /locus_tag="IYE22_002529"
     CDS             complement(814..1272)
                     /locus_tag="IYE22_002529"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ9507712.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1443..2588)
                     /locus_tag="IYE22_002530"
     CDS             complement(1443..2588)
                     /locus_tag="IYE22_002530"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ9507713.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2601..3518)
                     /locus_tag="IYE22_002531"
     CDS             complement(2601..3518)
                     /locus_tag="IYE22_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ9507714.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3946..4509
                     /gene="efp"
                     /locus_tag="IYE22_002532"
     CDS             3946..4509
                     /gene="efp"
                     /locus_tag="IYE22_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ9507715.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1468 a          868 c          909 g         1471 t
ORIGIN      
        1 aaagataaca ccacagtgtg gtggcgatag cgagaaggat acacctgtaa ccatgccgaa
       61 cacagaagtt aagcttctta gcgccgattg tagtgagggg ttgccccttg tgagagtagg
      121 acgtcgccac gcaaaggcaa aaagatgaac tagaaatagt tcatcttttt ttatttttat
      181 taagttaaag aacggattca attgcagcgg tcattttcaa tggagaagtt tgtggtgcaa
      241 atcgttcgat tactttgcca tttctatcaa tcaaaaattt agtaaaattc cattttattg
      301 ctttagtgac agtaccagga gcttcatttt taagataagt aaaaagagga tctgtatttt
      361 ctccatttac gtcaatcaat tgatgcatag ggaaggtaac gccataagtg gttttacaag
      421 cttctgctgc ttcagtcgct gaatctagct cttgtttaaa ttgattggaa ggaaagccta
      481 agataaccag accttgtgtc tgataagctt ggtaaagttc ttctaattgt ttaaattgag
      541 gtgctagacc gcattttgtt gcagtgttaa caatgattat cggtttgcct gcatattttt
      601 tcaatgaata gacagtgccg tcttctaaag tagtttcaaa attataaata gacataaagt
      661 tttctccttt ctattttata taataaaaag agcgctaaac tgaaaactag ctatttgttt
      721 tcccgtttag aactcttttc agacaataaa tatttaataa aatacgaaga taagatagct
      781 atattgcttc tcatctttac gttcttttag aaactatgct tctacttcag accattcatc
      841 gcgttttgct aaaaattcac gagccaaagc ttgtgcacct tctagtgtat ggctggcagc
      901 ccagccacat tgtgtttcat tgcttgcagg tacttcagta gcttcaagaa catctttcat
      961 tgttgcttca agaacatcta aaatgtcttc gtagctttca tgattcaata cagtcaagta
     1021 aaagcctgtt tggcatccca ttggagacca gtctacgatg taatctgcgt gattgcggat
     1081 tagttcggca gtcaagtgct caagtgaatg tagacttggc atgtccatgt gttctttatt
     1141 tggttgttta aagcggacgt cgtatttaat gatgacatcg ccatgcgcac ccgtttttcg
     1201 atcagctaca cgtacataag gtgctttgac ttttgtatgg tcaagattaa aactttcgac
     1261 attcattctc atttgtaaga ctcctatctg tttgttcatt cgttttgatg cttttcacta
     1321 aagaattatt tagtgaaatt tacaaaaacg tctttctact tgattctaac acttatcgtt
     1381 tccaaggtgt agcaatctga aatatttttt agatttttct gctaagtcgt ttattgggac
     1441 ttctagttct tgattctgtc aaatgcctga gctaaatcgt ttatgagatc ctgaccatac
     1501 tcaattccta cagaaagacg gatcaagccg tcctttatgc cagcctcttc tcgtttttct
     1561 tttggaatag acgcatgagt catgacagaa ggaatttcca ctaaactttc tacgccacct
     1621 aaactttctg ctaaagtaaa tagttggagt gattcaacaa atggaatagc agattcttca
     1681 ttttttaaag taaaagaaat cattccgcta aagccgctca tctgctgctt agcaactcca
     1741 tggagttcat gatcgggtaa tcctggatag tagacctttt ctactgctgg atgagaaaat
     1801 agaaaatcag ctaccacaaa agcatttttt tgatgttcct ccatacgaac acttagtgtc
     1861 ttgattccgc gctgaagtaa ccagctatct tggggaccaa gtacggctcc gatagcattt
     1921 tggtaaaatc cgatctgatc ggctagatct ttatggttag aagtcactaa accagctact
     1981 acatcgctgt gtccgcctag atatttggta ccgctatgga caacgatatc agaaccgaga
     2041 tctaaaggtc tttgaaaata aggagtagca aatgtattgt cagcaataac tatcaatcca
     2101 tgttgatgag ccagagtggc acttttttct aaatcagtaa tctttagaag cggattgcta
     2161 ggtgtttcaa gataaagtgc ttttgtattg ggtttgatgg attgttctat tttatcaaga
     2221 ttactcgtat ctatgatcgt gtactcgaga ccatttttag taagaacttt gtcaaataga
     2281 cggaatgttc ctccatagac atcatctcct aataagatat gatcgccagc ttgaaaaaga
     2341 gaaaaaacgg catgaatccc agaaagacca gaagaaaaag cgaatcctcg aacaccgcct
     2401 tccaaatcag caatcagttc ttccaatgca aaacgagtag gattaccaga tcttgaatat
     2461 tcatattgtt taggctggcc gacgccatct tgccgataag tggatgtttg ataaataggt
     2521 acactgacag ctcctgttgt gggatcttca ctgatgcctc cgtggatcaa ttttgtttga
     2581 atgtgcatgt ttctttcctc ctataagtaa atgttttttg ataaatagcg gtctgctgcg
     2641 tctggaaaaa tcgtaacgac acgatgacca ggtggtaaac gacgaatttc tcttaatgct
     2701 gctgcaaaag ccgcaccact cgaacttcca actaatagtc cttgttctcg tgctaactgc
     2761 ctagtatagt taaacccttc tacatcagag attgtctcga tctgattgat tgaaagggga
     2821 gaaagaaacg gcggaatgaa ttctacaccg ataccctcaa tttcgtgagg ggctggatca
     2881 ccaccattta acacagagcc ctctggttct acaccgatta ttcgaatgtc gggatatttt
     2941 tctttcaaaa aggtagaagt tccagcaaag gttccaccac tcccaattcc tgctacaaaa
     3001 ctatggatat tttctttgat ttcatgaaag atttctggcc ctaacgtatg atagtaagca
     3061 gcaggattat ctttattttc aaattgaaga ggcagatagc tgtttgatat ctcctctgca
     3121 agttttttac ttttgctgat ggcaccgatg attccttgct cactaggaga atggatgatc
     3181 tttgctccca atgctttcat caattgttgt ttttctaagc taaacttctc tggaacaaca
     3241 aaaaccgttc ttaatttgta tgtgagtgcg gccagagcga tcccaattcc tgtatttccg
     3301 gcagttggtt cgatgatcgt cgttttttca ttgatttttc cttgggcaaa tccttctttg
     3361 atcaaataac tgcccaatct atctttgata cttcctccag gatttaaata ttctagtttt
     3421 gcataaatag cagaaccttt aggcatagaa tagtgctgat tgtcaaagcg ataaagcggt
     3481 gtggaaccaa tagcttctgt aattgtcgag ataatcatgt gggcctccta aaaaaagata
     3541 ataaaaaaag gagctgacta ttaaatcagc tccttgaaag acatagagaa aaattgagta
     3601 gataaaaaga taagttacct ggtactcatt ctatgaaggg caattaatag acatgacaaa
     3661 tagtatgctg cttttatgca ccatacaaca gcaacgattc aattgttttt ggttgctgac
     3721 tgtcatgtgt atccctccaa gtaaaatttt tattaacgat agcatgaaaa aaaataaaag
     3781 tcaaaggctt gaaagaaaaa aatatttcga tagcatcgtt tacccgtgtt tatttttctg
     3841 acaaataggc atgagacctt aaaagataga gaatactcta gttttctggc cggttgtctg
     3901 atacaataaa gttgattatg ataaagaaaa gaaggagcaa aaataatgat ctcagcaagt
     3961 gatttaagag ccggtatgac tttcgtacaa gacggcaaac tgattaaagt tctggatgca
     4021 agccaccata aacctggtaa aggaaataca gttatgcgta tgaaactgaa agatgtccgt
     4081 tcaggtgcaa catacgacac aacgttcaga ccagaagaaa aatttgaaaa agcacatatc
     4141 gacacaaaac ctgtacaata tttgtacacg atggatgata cagctttctt catggatttg
     4201 gaaacatatg agcaatatga aattcctgta gaaacagtag aagaagaaat gaaatatatc
     4261 ctagaaaaca tggaagtaaa aatccaattt ttcggtagcg aagtcatcgg cgttcaatta
     4321 ccaacaactg tcgtattacg tgttgtggaa acacaacctt ccatcaaagg tgcaacagtc
     4381 actggttcag gtaagccagc gacaatggaa actggcttag tagtcaatgt tcctgacttc
     4441 atcgaaacag atgaattgtt ggaaatcaat acagcagaag gttcttatgt aaaacgtgcg
     4501 tcaaaataat tcgctaacgg attgataaga atttgaaaac agagcttgac ctcttgaaag
     4561 gcagatcatc ggatctgcct ttcaagaggt tttttgtgga atcagcaaat tgaaaaataa
     4621 aaaagtattt gattttattt atattttgaa agatgaatgg caaaagctag agaaagaaaa
     4681 aactgcactt atttatgaag aaattgaaat gattca
//
LOCUS       DACIRY010000163         4714 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.170, whole genome shotgun sequence.
ACCESSION   DACIRY010000163 DACIRY010000000
VERSION     DACIRY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.170"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            229..483
                     /locus_tag="IYE22_002533"
     CDS             229..483
                     /locus_tag="IYE22_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ9507716.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(572..1618)
                     /gene="holA"
                     /locus_tag="IYE22_002534"
     CDS             complement(572..1618)
                     /gene="holA"
                     /locus_tag="IYE22_002534"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ9507717.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(1656..3929)
                     /locus_tag="IYE22_002535"
     CDS             complement(1656..3929)
                     /locus_tag="IYE22_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ9507718.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(3922..4461)
                     /locus_tag="IYE22_002536"
     CDS             complement(3922..4461)
                     /locus_tag="IYE22_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ9507719.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(4597..>4714)
                     /locus_tag="IYE22_002537"
     CDS             complement(4597..>4714)
                     /locus_tag="IYE22_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ9507720.1"
                     /translation="IKEYISVLTEVAKRHDCEVSIFIRNFLNKWIRRIFIMG"
BASE COUNT         1515 a          965 c          758 g         1476 t
ORIGIN      
        1 aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt tttgattttt
       61 ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact gttaaggaaa
      121 aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa tgaatgttga
      181 attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat gccaaacatt
      241 gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa ttcatctcaa
      301 acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc aggtgctgac
      361 aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga atcaaaaggt
      421 ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa aatcgctaaa
      481 taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa aacaaacgac
      541 cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac tgccctgtgc
      601 gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg atttgataat
      661 ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat ttttttactt
      721 cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca atatttgcct
      781 gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat tgagaaagca
      841 aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg tataaacgca
      901 aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata ttatgttcta
      961 acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga ttttgtccat
     1021 aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg gtcaacctca
     1081 agaataaatc aattgcactt cgttccattt tgattgagga attctctaag gtattcatca
     1141 gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt gctgtttttt
     1201 tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca aagaatacta
     1261 atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta ttttgttcga
     1321 ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat actaaccgtt
     1381 gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa cttgcatttt
     1441 ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt tttttgacaa
     1501 tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag taacaagatg
     1561 caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta ctcaccactt
     1621 tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt ttccactagc
     1681 ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg atccgttcgc
     1741 catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg gtgaccataa
     1801 cgattattta cgccgcagga tataatccca tgttttgctt caatatggga aagaaaatct
     1861 gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc tttcagatta
     1921 ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa aaataaaaag
     1981 cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc ctctcccttt
     2041 tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc atgtttgatt
     2101 tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa tggctcattt
     2161 cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga taacgtgggt
     2221 aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat tacacttatc
     2281 ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat cataccttgc
     2341 gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat cgtttctttt
     2401 cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa agcgatcgtt
     2461 gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc aacataaaac
     2521 aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc gaaaatatac
     2581 atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga agaaaaatcg
     2641 attattcgct caaataagat taatccctct tctatcagca aaagtaaagt ttccggcagc
     2701 cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt gaataccggt
     2761 gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg ccatacaatc
     2821 aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa ccaatctcca
     2881 tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc acccgcatga
     2941 aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa taagtccatg
     3001 gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag caaactagca
     3061 cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag tagactcaac
     3121 ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata cagccaccct
     3181 aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc actctcccta
     3241 aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa agaattaata
     3301 tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga tcctcttttt
     3361 ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt ttgaatttgg
     3421 aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt ataaagattc
     3481 cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc ctttcctcgg
     3541 aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag ctggagcttt
     3601 tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc acgaatcgta
     3661 tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga atactcttgt
     3721 agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc gataaaacgt
     3781 ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata accccaattg
     3841 tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat caaccgattt
     3901 cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt gaagttttga
     3961 aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt cgatcaacgc
     4021 ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca caccagcttg
     4081 caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt ctgaaccttc
     4141 cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat ggatcgttcg
     4201 caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat ctccactgac
     4261 tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg ctcctacttc
     4321 taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa agtactggtc
     4381 ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg tatcgcatgc
     4441 ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg attaaaaagt
     4501 actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct gacaaaagat
     4561 cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa aaattcttcg
     4621 aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat ggcgttttgc
     4681 tacttctgtg agaacagaga tatattcttt gatc
//
LOCUS       DACIRY010000164         4658 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.171, whole genome shotgun sequence.
ACCESSION   DACIRY010000164 DACIRY010000000
VERSION     DACIRY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.171"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(119..206)
                     /locus_tag="IYE22_002538"
     tRNA            complement(119..206)
                     /locus_tag="IYE22_002538"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Ser,seq:cga)
     gene            complement(351..917)
                     /locus_tag="IYE22_002539"
     CDS             complement(351..917)
                     /locus_tag="IYE22_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9507721.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            1172..2503
                     /locus_tag="IYE22_002540"
     CDS             1172..2503
                     /locus_tag="IYE22_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ9507722.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2469..2819
                     /locus_tag="IYE22_002541"
     CDS             2469..2819
                     /locus_tag="IYE22_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507723.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2911..3330)
                     /locus_tag="IYE22_002542"
     CDS             complement(2911..3330)
                     /locus_tag="IYE22_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ9507724.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(3331..3675)
                     /locus_tag="IYE22_002543"
     CDS             complement(3331..3675)
                     /locus_tag="IYE22_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ9507725.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            4025..4621
                     /locus_tag="IYE22_002544"
     CDS             4025..4621
                     /locus_tag="IYE22_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ9507726.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1257 a          865 c          812 g         1724 t
ORIGIN      
        1 gcttgcatct gcggtaataa gcccaaccaa acgaaaaaat aggaaaagct atttttgttt
       61 ggccgttctt attacttgat gcaggacagc ttcttcacaa cctctaatcg ttgatataag
      121 gaaggtacag gatttgaacc tgcgcgccgg tattagccgg ttcgccggat ttcgagtccg
      181 gtgcattacc actctgccaa ccttccgtaa gtactatttt taaacttttt tcacgaaatg
      241 tcaagcctat ttctaataga taaataacag aaatcgcttt ttttagcaaa aaagtccatt
      301 gacagtttgc agccaacgga cttaccgatc aaattcatac tgatagaaca ctatctgatt
      361 atttcaggat tatctaatgc taaaatcgag tcaattgtcc gtttgatcag tgcaatgatt
      421 tgttcttctg ttggatgcat gatgtttgat gacatcaatg acgctaagcc agtcactgct
      481 acccatgtac cagtatgcag agcatcgaca tattcatccg ataattgatt gtatctttca
      541 tcttctttaa ctatttcacg gaaataattg tatgaaaaat catgcatttt ctttccgtcc
      601 ccatgttcat caatgtacaa tgccatgtaa agactatgat gcttttttgc aaagtagata
      661 tagttcaaac cgagatcaat gattttatca ccagtacgtt caatagggaa aacatcttct
      721 tttagttgtt caaagataga atccagcaac gtatttttta aatcttccat gtttttgaat
      781 tccagataaa tcggctgtgt cgaaatcccc atctttttag caatatttct cgcagtaaat
      841 cctttaaacc cttcctttgc aacaacatca taagcagctt ttagaatttg atccttggta
      901 tagacttttc ttctcattaa aactccccca cttctttact gcagtatatg ttgttttatt
      961 gtaccctatt tttagcattt tttgcaaaca ttttactcaa aacgttatat tttaaatttt
     1021 tttcagtctt ctttaacttt tttttgcaaa attaaaaaaa tttggttaaa ttcaatgggt
     1081 tcccttacat ttttagtttt gacctaaatg atagttgtcc aaatctttca tttcaagtta
     1141 tagttaaaga aaatgaaaaa gaaggtgtgt aatggaaaac gaaactgacg ttatttatat
     1201 tcatcctcaa aaacgaatcg tttcacagaa aagaaagtat ttttatcttg gttttacagg
     1261 tgtttttttt ctttttattg gcttgctttc taatactcct actgataatt ggagtggatt
     1321 gctaactatt cttacctcgc caagcaatct tttgacagat tattttgctt taggtgtttt
     1381 cggaagcgca ttcataaatg tagggatttt gaccttactt tctgtgcttt tagcttatcg
     1441 gcacaaggtg attttaaacg gtcccttatt tgcttcgatt ctaacagtta ccggtttctc
     1501 tttttttggc aaaaaccttt ataattctat ctcaatcatt ttaggtgtgt atctatacgc
     1561 tgtatttgtc aataaacctt tttctcagta catcatgatc ggactgtttg gtagcgtatt
     1621 atcaccggtc gttagttata tcacttttgg tatgcgtttt cctttactga taggtatttt
     1681 attggggaat ttagctggca tagcaattgg tatacttctt ccgcccttag ctgctcaaac
     1741 tttagttttc catcgtggat tcacacttta taatattgga ttcacctctg ggttgattgc
     1801 gatgactttt actgctgttt tgaggttatt cagctattca atcgtcgaaa atacattggt
     1861 tttcaatgaa tatcactttc ctctcatttg gattattttt ggcttttttt cgctgacagt
     1921 cggaattggt ttctattata attcctttcg gctttcgggg attcgagaaa ttttcgattc
     1981 atctggaaaa ttgacaactg atttcattgc taattctggt attggtgcta ctttgatcaa
     2041 catgggctta gttggtctga tgctgtcttc ttatgttttg ctggttggcg gtcagttaaa
     2101 tggacctgtc atcggtgcta ttttatctgc ggtgggtttc agtgcttttg gttgtcattt
     2161 aaaaaacagt tttccgatac tagtcggaat atttattgct tctttgtttg gaacttttca
     2221 tgagatcacc agtactggca tgcttgttgc agctgtgttt gggacaggtc ttgcgcccat
     2281 aagcggtttt tacggcagtt tttacggtgt aatagctggt gtgctacata ttgctcttgt
     2341 ccataacgtc agcactttgc acggaggact aaatctatat aacagtggtt tttcaactgg
     2401 tttcgttgca ggtatccttg taccgatact agataacttt accgctgtca gaaaggaaaa
     2461 aaagacactt ggaaaaagaa ttatcaaaaa gaatcatcga tgagcttctt acttatttat
     2521 attcccatga aataactgaa atacgtattg gagtagaatt tgctgaggaa gggcttttca
     2581 tagagataca gggtacggca gaaactcagc cagaagattt agatgtactt gctgatttgc
     2641 tgaacaatcc tcgggaccct agtatggaaa tctactatca agaactatta ggtaatcccc
     2701 gacatgatcg ggaagattat catctgttag gtagtttgat tgatgacgtg gaaatcatgt
     2761 atgcccagcc gattatcaat attaagatct ttcgacgtgc ttctcattcc tattattaac
     2821 cctattttct atttataaaa gactgaatca aaaaagcagc tcctgaaagt tcgattaaaa
     2881 tctactttca ggagctgttc aagcttgaca ttattgattt tccaatcctt catacacttc
     2941 atctgttaca ggttctaaaa actctgaatt accagcagtg atcgccaaat gttcaaacca
     3001 actgtctttt gtcgcaccat gccaatgttt aatccctttg tgcgttacga cgacatctcc
     3061 agccattaat cgtcttgctg gctttccttc ttcctgatac cagccttctc ctccggtcac
     3121 aagtaagatt tgatagccgt ctgtatggac atgccagtga ttgcgacaac ctggttcaaa
     3181 agttacatta cctacaccaa cagaaacttc gggatcattc actaacatat tcagatagct
     3241 ttgcccggta aatacttgag cgtaagcttc gtttttttca cctgctggaa aaatcacgcc
     3301 attgtgtatt tcttcgtgtt ttgtcatcat ttacgcctct ttcttatctt cgctattttc
     3361 ttctaaatag atttcttttg ctaaactaaa agctgaccaa gcttttggcc attctacata
     3421 aaaagctaaa tgggtaatga tttctgctat ttcttcacta gtaatgccat tttgcttgcc
     3481 aatcttcaga tgagctggca attgttcaaa atttcctcct gaaattaatg cagatatggt
     3541 gatgatacta cgctgatgag cagataattc ttctctggac catacttgtc caaataaaac
     3601 atcatcattt aattctgcaa attttggtgc aaaatctcct aattgatctc gtcctgcggt
     3661 ttgtttttct gccatttctc ttattctccc ttcacgactg ttaggtgatt caactatggt
     3721 tctaaggcaa ctttattttc tattgtttct tcgcatttca ttttaaatgt agtctttcag
     3781 cctgtccaat gcttagttta ttcagtcgat atgcttttta agcatagttg ctaacgcttt
     3841 ttttatttta aaaaaaacaa gttattaaat aagtctttag ttggatgttt ttttgagatc
     3901 aatcttttat acttatgatg aacttacaaa attgtttgtt ttctgtcata tcaaaaaatg
     3961 gcgcagttag ttgcttttct agtaaagtat agttaacaaa gaacctctaa ggagttgatg
     4021 aataatgaat attgctgcct ctcgaaaact gataaatttc atatccatta tcggattagg
     4081 cctatcaatc gcgctgacga tttattttat taatttaggt gttttcaaag atttgaatgc
     4141 gttaagagga ttagtaggtg attccatcat tttaggaccg attatttttg tcttcatcca
     4201 aatcctgcaa gtagttattc caatcattcc tggagggatc agtactgcag ctggtgtcct
     4261 gatatttggc ccttatgctg gatttatcta taactatatt ggaatctgca ttggttcgat
     4321 tatcattttt ctgctagggc gtcgttatgg aaagccattc atactttcca tgattagcga
     4381 taagacatat aacaaatata ttggctggct ggataatcaa aaccgttttg agaagctgtt
     4441 tgctctagct atttttcttc cagttgctcc tgacgatgcg ttatgtttga tggctggatt
     4501 gacaaacatg tcagtgaaac gatatacact aatcattttg attgctaaac cactgtcgat
     4561 tttcttatat agtatggcct tgatttatgg aggtcaatat ctaagtggtc ttcttggata
     4621 atatttattt tgtaaaatta gtcattgatt gacaatgt
//
LOCUS       DACIRY010000165         4614 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.172, whole genome shotgun sequence.
ACCESSION   DACIRY010000165 DACIRY010000000
VERSION     DACIRY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.172"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..126)
                     /locus_tag="IYE22_002545"
     CDS             complement(<1..126)
                     /locus_tag="IYE22_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ9507727.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQS"
     gene            complement(286..603)
                     /locus_tag="IYE22_002546"
     CDS             complement(286..603)
                     /locus_tag="IYE22_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507728.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            complement(741..2969)
                     /gene="topB"
                     /locus_tag="IYE22_002547"
     CDS             complement(741..2969)
                     /gene="topB"
                     /locus_tag="IYE22_002547"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ9507729.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVILYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(3143..3553)
                     /locus_tag="IYE22_002548"
     CDS             complement(3143..3553)
                     /locus_tag="IYE22_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ9507730.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(3670..4485)
                     /locus_tag="IYE22_002549"
     CDS             complement(3670..4485)
                     /locus_tag="IYE22_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ9507731.1"
                     /translation="MGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
BASE COUNT         1212 a          980 c          760 g         1662 t
ORIGIN      
        1 agattgaaaa acataactga ttgcgtcacc tgtctgcaca cccatgaatt cttttacttc
       61 tggtgggata gttgttacat cgctattgcc agattttcta attttatact ttttcatttt
      121 tttcatattt cgccaccctc tataccattc tcattctata gtaagtacat tatatccaag
      181 tatatacttt taatcaatgt atatactttt attttgcttt ttcttacgaa aaaatgacaa
      241 agaaaaaagt ttgtgacatg acttttgtca caaactctcg tattcttaac caaaatattg
      301 atgaaatact tgtgtaataa ctggtagaaa ataaataatg atcatagccg ctaccaccac
      361 caacaattgt tttcgctgtt tctttcgctt tagcatgctt gtcgttttat gaaaaaaata
      421 agtagcgctt ttagctcctg cagtcaaagt cgataaaatc aacgttggtg aatatgctgt
      481 ttgattgtgc gtcaatgaca acaacttcgt cccgtaatca ggatacttta gaaatgcttg
      541 gtacacagcc acaatcgtga ttgctcccat aagataattt aaaaatgaat gcactttttt
      601 caaataaaaa aactccttgc tatctatata aaatcagcct accccaacgt ttttgcttga
      661 ttcactgata ttttttagtt ttattggtat ttatattctc acttttcgga ataaatattc
      721 cgctatttca ttgttttcat ttactctact tcgttttcat tattttttct tgatgtttct
      781 ttgtttccat caactgcttt tgttccactg ttttcgatcc atggaaaaga aaaaccgatt
      841 gtcatatctt cttttaatac taaaactgca tcgaatttcc cttttttacc agtaaaacct
      901 ttgacaactt tacttgtctt tccttttgaa agcaaatctc tgagattcgt tttagtcagc
      961 ttctttttag ctactgttgg ccacaatttg aagtcgcatt ccggattcaa gcatgtagcg
     1021 attttaggat aaagaatgac tattcccttt ttacattttg gacatcttcc taattgatcg
     1081 tttttcttct ccatcgcttt tatctgacgt gtcccgctaa tctcatctga aaagtctacc
     1141 ttgtcgattt gattaggaac ttctgccaat aaatgtagga tgaatttttt gatattctct
     1201 aaaaaaacag atggtgtccc ctcttgttta ccaatttttc tcaagtaccc ttcccagcgc
     1261 gcagtcattt ctgcgctcgt cagtaatggt tcattggcta ctgccttaca aagagtgatt
     1321 ccttttggcg taacagttat ttcgttttta ttgacttcga tgtaatgctt ctgcttaaga
     1381 ttttcaatga tattggctcg tgtagcttct gtccctattc cttctacttc tttgagaatt
     1441 tcttgcgctt catcattatc cagcgttttt cccgcagttt tcattgctgt gatcaacgtt
     1501 ccttcggtat agggttttgg aggttgtgtt tctttctcca tacttttcag ttctacttct
     1561 gcttcttcat tttctacgat aggaggaaga gtctgagctt ctttcttttt tgacttgatg
     1621 cgtaagattt cctgccaacc ttgctgtaat gggatctttc cgatgctttt tagctttaat
     1681 tgctgtacgc ccgtcattac ggtcgtctct tcgtagatat aatctgcagc aaacatagcc
     1741 acagtagtgc gcaaaacttg caaatagatt gcttgctgta atttggaaag cttcgcaaag
     1801 cgtgcaggag tcggaatcgt ctttgtcgga ataattgcat gatgttcttg aacttttttc
     1861 gcatcgacat atcgtttctt tgctttgatc tcagttgtct gtacttcttc tattcctaaa
     1921 aattttttat aattttgcag atgtttaacc agatactgat gctcattttc tgtaatgtaa
     1981 aaggaatccg ttcttggata gcttagaaac ttgctttcgt acaatccttg agcagcatca
     2041 agtgtagctt tggcacttgc tttcattagc tgattcatct ttgtctgcaa actagatagc
     2101 gaaaataaac gtggactatg caacctcttt tcctttttcg taacttcaag aatccgtccg
     2161 ttttgcttac ctaaatgcgc tccaagtttt tctatttcct tcgttaactc ttcttgggtt
     2221 ttaaatgctt tttttggtac gatttttcca gaaaactgcc ctttgtttgt cgtaattaaa
     2281 ccttctcctt caaagtacgt ttcttttttg aagtgtttga tttcttcttg cagctgataa
     2341 atcatataga gtgtcggtgt ttgtacacga ccaagagaaa aactgccatc aatccctttt
     2401 cgttgaagta gcaagctata taatggactt ccattcattc caatcagcca atcagcgttc
     2461 tgccttgctt gcgcttcttt atattttgga aaatagttct ttccaggttg gagattctta
     2521 aaaccttcat agattgcttc tttttcaaga gaattgatcc ataaacgtaa atagttcttt
     2581 tccttagaaa atgcgtttgc ttgtcgaata atcgaccacg cgatggcttc accttctcga
     2641 tcaccatctg ttgcaataat gatggtatcg gctgactgca attctcgttt tacgatcgaa
     2701 aattgtgcac gtttgtccat tggaacttcg taacggaagg tttctgggaa aataggcaaa
     2761 ttttctaaag accattttgc ccaacgctct tcataagctc ctggagcaac catatctacc
     2821 aaatgtccaa aaccataagt gatggttact tcaccagcaa acaatggatc gctgatttca
     2881 taataccctt catgccgctt ttgctgttgg aaggtttcgg cataggcttt tgcttgtgaa
     2941 ggtttttcag ctagtatcac cgtcttcatc ttctgacctc cgttacttaa ttttttgcgg
     3001 acttctctat tatggaacat attttcgcat ctggcaataa aaaaaagata ccgtatagtc
     3061 gaaatagaca caaaaacatc aagcaaacaa acgatgatcc gtttgctgct tgatgtttcc
     3121 agaaaatatt catctttagc tgtcactctt ggttaatctg ccagttgatg ccaaatcgat
     3181 cagttaccat cccatacaac tgagcccacg gaacagcatc cagtggcatc tcaatcttac
     3241 caccctcagc taagctttca aataggcgct tcgtttcttc ttcatttctt gtctggatga
     3301 ctaaagaaac attatttccc acaactaatt caggaccaat tccttctggc gtatctgcaa
     3361 acataactaa tgtatcataa atgttcagcc gtgcattcgt gattagattt tttgcttctt
     3421 ctggcaagtt catttgttct attccaggca tttcaccgta tgtggtgata cctgatgctt
     3481 cagtctgaaa aactttttca taaaaagcga tggcttcacg cgactcattt ttgaagttca
     3541 gatagatatc caatgtaaac agctcctctc ttggttaaat ctaccataag aaagcgtttt
     3601 ttacgagtga atcgtttgat tgattcatga caaaagcctt gcgcagcttc ttcttgatct
     3661 tttttatgat catttattca aaaaatctgt aatagcagat tgattcattt gattatcaat
     3721 ttcaagaata ctgccagcat aatcactttg cccataattc cacgaaccgt ctactggcac
     3781 acttattcga tcaatgccac cagctcctcg tgcaatcgaa aagaggtttt tcgtcaagaa
     3841 ccaagcagtg acatctgtgg atgtatatcc taaagcttta ccagctgcat aaggtgcacg
     3901 gatcagattc aatggattct tcaattggct gaaaatagcg ttcattactt gctgttggcg
     3961 tctgacacgg ccaaagtctc cttgttcatc cattcggaaa cgtgcgtact gcaaaagaac
     4021 atgtccatcc atcttctgga cccctttttt aatatctaca ccatccagat tcagatcttt
     4081 ttctgcatcg atcttcactc cgttcatgaa taaggcatcg atcacttttt caaacgattt
     4141 gaaatcgact ttcgcatagt attgacaatc gattccaaaa ttttctacta aagtttgacg
     4201 cactagatcg gctcctccgt atgcataagc tgaattgatc ttattttgtc ctacccctgg
     4261 gatcgtaacg aagctgtcac gcataaaaga aatcagtttc ggcttatggg aaggaccatc
     4321 caactgtaag accatgatcg tatcggctcg cccggcatct tctccgcgtg tatcacttcc
     4381 aaggatcaaa atattatgcg caccattcgt cgctttttgc ccatgaaagg tttctgaagt
     4441 cgctgcctct aagcttgtgt catgttcagc tacatattta ccaataaaaa atgagatacc
     4501 tgaataaagg atcaaaacaa gcaaaatcga tagaatgatt tttttccacg atcgctttct
     4561 tcgaggtttt aacggtttgt tcttcttttg ccgattgaaa aattttttat tttt
//
LOCUS       DACIRY010000166         4600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.173, whole genome shotgun sequence.
ACCESSION   DACIRY010000166 DACIRY010000000
VERSION     DACIRY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.173"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..307)
                     /locus_tag="IYE22_002550"
     CDS             complement(<1..307)
                     /locus_tag="IYE22_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9507732.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     "
     gene            642..1076
                     /locus_tag="IYE22_002551"
     CDS             642..1076
                     /locus_tag="IYE22_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ9507733.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1099..1419
                     /locus_tag="IYE22_002552"
     CDS             1099..1419
                     /locus_tag="IYE22_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507734.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1560..1751
                     /locus_tag="IYE22_002553"
     CDS             1560..1751
                     /locus_tag="IYE22_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507735.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1822..2079)
                     /locus_tag="IYE22_002554"
     CDS             complement(1822..2079)
                     /locus_tag="IYE22_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ9507736.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2237..2500
                     /locus_tag="IYE22_002555"
     CDS             2237..2500
                     /locus_tag="IYE22_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507737.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2524..2712
                     /locus_tag="IYE22_002556"
     CDS             2524..2712
                     /locus_tag="IYE22_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507738.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2795..2980)
                     /locus_tag="IYE22_002557"
     CDS             complement(2795..2980)
                     /locus_tag="IYE22_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507739.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3195..3566
                     /locus_tag="IYE22_002558"
     CDS             3195..3566
                     /locus_tag="IYE22_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ9507740.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3716..3788)
                     /locus_tag="IYE22_002559"
     tRNA            complement(3716..3788)
                     /locus_tag="IYE22_002559"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3753..3755),aa:Gln,seq:ctg)
     gene            complement(3834..4262)
                     /locus_tag="IYE22_002560"
     CDS             complement(3834..4262)
                     /locus_tag="IYE22_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507741.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4322..4597)
                     /locus_tag="IYE22_002561"
     CDS             complement(4322..4597)
                     /locus_tag="IYE22_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507742.1"
                     /translation="MKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVH
                     ACVVTGYDKENIYVNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1616 a          706 c          911 g         1367 t
ORIGIN      
        1 caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt
       61 gtgcagagcc ttctgttggg attgttaatt gttgtcctga gtagatcagg ttaatatcag
      121 aaattgaatt tgattctgca atggcattga tcaaggagtt atcaccaacg tatttttgag
      181 aaatcgttga tagagtatca cctgattgaa cggtataagc ttcgtccgca tgagcagtcg
      241 ttcccatgaa gaatgcagcg ccggcagcca aagttgttcc aaaaagtaaa gttttaagtg
      301 atgtcatgaa aaatctcctt tattcgttaa aaagttttat ttgttgtgtt caacgaggaa
      361 tactttaaca cgtgaaaata tttcataggt aaaagaagca tgacaattta tgacaaatcc
      421 acgttttttg taacagaatg atgttcgctc gatgtgaaaa atgatgagga atttcacata
      481 atgcagaaat aatgggatta gaacaggtgt tctgatgaga aaagaaatgt ataaataact
      541 tgaaagaaat tgcttttgtt catcatttta tttacatatt gcgtatattt tttgtatttt
      601 tgcttgagat gttacaataa aagaaaaagg aggcggttcc aatgagtaaa agataccaga
      661 acatcatggt agcagttgac ggatcaagac aatcgatcaa agcgttcgct gaagcccttg
      721 acttagcaaa ggacaatgaa gcccacttat ttatcgtgtc aatcatcaac aaagttgagc
      781 tgacccatag tgcctatgcc ttttcaaaaa tctatgcaga tgaaaaacaa agaacagaag
      841 tagccatgct gaaaaaaatc aatgatgcca aagagtacgg cattgctgag atccacgcaa
      901 tagttgaaac tggagatcca agaaatttga ttgccaatgt catccctgga caagaaaata
      961 ttgatttgat cgttatggga gcgacaggaa aaggtgctat acaacaagca ttagttggat
     1021 cgaccgcttc ttatgtcgta tcacatgccc cttgcagtgt gttggtcgtg aagtaatgag
     1081 ataagggagg aatagaaaat ggttcgtcct caagaagtcc gtgcgccaaa agaaaaaata
     1141 gaaattttag caattattga agacggcaca caaacaaaga agggatactc gatcgcttta
     1201 gtaaagtgga aaggaaaaaa aggtgttgct ctgagatggg atggagataa ccaacaagat
     1261 aaaggattcc ctgtcacagc taacggttat catccagcgt ggtttgttct gcctgataaa
     1321 tttatggaat tatacagcta tgattatgca agagccgcag catcaatcaa agcattagat
     1381 aaattaacag aagagcagaa tgaaatggag gaaaaataaa aaagacgaac caatcagcct
     1441 ttagaaaact gattagttcg aggagtaaaa atgaaaaaag gttatttggg atgaaactag
     1501 aataatgcgt ataacttaaa taaaacttaa agcaataaaa tggcaagagg gtgaggcaaa
     1561 tggaagcagg aaaagtatta caggaagtaa aagcactatg tgcagataaa aaatatgaag
     1621 aagcgaaaag cctgatcgaa aagaacaaag aaaatttgga agaaaagtac ccgatcgcac
     1681 aagaatttat agaattaaaa caaggaacga tccttcaccg attcaaacat ttttttggta
     1741 taacagaata gaaaaaacga ttaaagatcg gcagaaattt gctgcatctt taatcgtttt
     1801 ctttttaaac atgcgaggga ttcaattctg attcttatat aaaaaataga tccaaataat
     1861 aagaaaaatt actgaaagcg tcaattcgat gatgtgtgtt attaatacag acgttgtttc
     1921 ccaatgccta aaagaaaaaa tttgataagt aaaatataca gcaggtaaag aggccagtaa
     1981 aactgaaaac ttcttttttg tccattttat gtaatagaaa agaatgctta tcataataaa
     2041 aaaaggaata taaaaaacaa attgaccgat cgcgatcata ctattgcctc cgataaataa
     2101 agtccatgat acaaaataac actttgtgcg aaatggcgca agcagttttg tggatttatc
     2161 cggtaaaatc tgtatcgatt ttgaaattag aggagaaact tgctataatc aaagtgaaag
     2221 aaggagtggg attttcatgg atcatgtagt gttagaaatc gatttgcatt tgatcaataa
     2281 tattcgggtg atttactatg ttgtaagtaa cagtgtcgaa caacgtgtac tgacgaataa
     2341 aataaatggt attttagcaa agaaagacgt tcaccgattc aataatggtg aaggaagtta
     2401 ttatagtatc ccagtagaaa aaattattta tacgacagtc aaagtcagag aagatctaga
     2461 taccaaaaaa gcatatgaac ctatttttac aacttattga ttgacagcag atggaggaaa
     2521 aaaatgaatc catatgaaca acttattcaa gatttgatcg atggaaaaat cgaaaagatt
     2581 gatgtaaaaa gagaagaatt aatggctttt cgagaagcgt ggctgaagtt ggagaatcgt
     2641 aaatatgtgg tgggaaaagc ctcactgaac ggcaatgtga cttatcatta cgatccgaca
     2701 cgtttatttt agtatgaagt attcagaaaa gaaccaaagg aggacgatgc agcagcattg
     2761 tcctcctttg attttacaaa agtgaaagtt ttacttattt gaatagattt ttaaatttgt
     2821 ttttgacact gtcgatattt acatctttta aaagattttg ggctttctct ttataaggtc
     2881 ctaattgttc tttatgttct tcgataaatc gttttgcgcc atcgaaattg ccgttcttaa
     2941 tcatttcctt gactttttga aataattctt cttgattcat tttgaacaca tcctttgcta
     3001 tcaacatgat tttttacatg gacaaaacga gaaaaactga tttatccaca cgtcaataat
     3061 atcaaataac gatcattttt actaaaaaaa cactcgattc tcgcttctaa agtaggagaa
     3121 aaatctccaa cttgggacgg atggaatttc tttaactgac ccatatactg aatatataac
     3181 aaggaggtag aaagatgaaa gtgattaaat atttgattgg actggcggcg attagtggta
     3241 ttttgctatt tggactgaaa tattatactg aaggaagtta tggagaaatc cctggtatac
     3301 ttgatcagct aaatccatta gttgaaaaag gagaggtcta tgtaaagaca caaaaaccag
     3361 aggaagtaaa tgaatatggt acagcaaggt atgtgcagaa agctgctgat gccaatggga
     3421 aagaacgaac ggttgaattt aatggtcttt ctgtgttaaa agaaaatcat tatttgaaaa
     3481 ttagcaataa aggagcacac atagaaacgt atgaggaagt tcctagagaa aaagttccgg
     3541 agaaagcatt agcaattatt gggtaaatca gtttttgtat taagaatacg cccgatttta
     3601 ttattcataa tttaattatg taaactaaat tctagtagta aaaataatac aataatcccc
     3661 ctgcaaaaag attgttcttt tacagggggt tcattttttc agcaagcaca tcaaattggg
     3721 atggcagggt tcgaacctac aattgcttga ttcagagtca agtgccttac cagtttggct
     3781 acatcccatt tattcaacaa aataatcata gcactttcct aaaaaaacgc aagttatttt
     3841 tcttgatcgg atagttgttg ctttactcgt aaaaggcggc gtactagttt cttttctaat
     3901 ccagcaggaa gggcaatcag ttctgctact gacaagacgg tgtccaataa ctgatattct
     3961 ttgacaggtt cattcataat atgatagatg tttgctaaac gttcaatata ttcaaagcga
     4021 gtatactctg ttcgagcaat tagaaatttc aataaatcgg tggctttctc gtactcttga
     4081 tctgcgtaat aaacaagaga ttgacgatac aatttgattt ccagttcatt gagggtagca
     4141 ggtctgcgac gctcgttcca ctcattaata gagactttac cttgaacatt tcccttgcag
     4201 tcacggacaa aaataatcgg aatttcattt ggatccattt ttttatgtgt gaatttttcc
     4261 attatctacg ctcccttcaa aggtttggta tcttgagtat aagggatttt tgtttgaatt
     4321 actagctttt ctccaaagtt agcatctgtc cacccattgc agtgaaaatc gccgaaaaat
     4381 cgttgatcgg tactgctcga ttttttacac catagggatc gttgacataa atattttctt
     4441 tgtcatatcc agtgactaca caagcatgaa caagaggagt gactcttact tctccattat
     4501 ttgtggtcca agtcataaaa tcagtttcat ctgggacttc aaaattgata gtcacagaag
     4561 tccagacagg atttccttta gatagtgctt ttttgatatc
//
LOCUS       DACIRY010000167         4497 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.174, whole genome shotgun sequence.
ACCESSION   DACIRY010000167 DACIRY010000000
VERSION     DACIRY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.174"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            188..418
                     /locus_tag="IYE22_002562"
     CDS             188..418
                     /locus_tag="IYE22_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ9507743.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            515..1816
                     /gene="murA"
                     /locus_tag="IYE22_002563"
     CDS             515..1816
                     /gene="murA"
                     /locus_tag="IYE22_002563"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ9507744.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1829..2005
                     /locus_tag="IYE22_002564"
     CDS             1829..2005
                     /locus_tag="IYE22_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ9507745.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2140..2436
                     /gene="yidD"
                     /locus_tag="IYE22_002565"
     CDS             2140..2436
                     /gene="yidD"
                     /locus_tag="IYE22_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ9507746.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2621..3088
                     /locus_tag="IYE22_002566"
     CDS             2621..3088
                     /locus_tag="IYE22_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507747.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3173..4057
                     /locus_tag="IYE22_002567"
     CDS             3173..4057
                     /locus_tag="IYE22_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ9507748.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4407
                     /locus_tag="IYE22_002568"
     CDS             4174..4407
                     /locus_tag="IYE22_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ9507749.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1536 a          684 c          980 g         1297 t
ORIGIN      
        1 tttggaataa gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag
       61 catttatttc ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct
      121 tttatacatt ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga
      181 cgtcattatg caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt
      241 tatctactta gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat
      301 gcataatacg caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc
      361 aagtaatttt ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat
      421 tttaaaaaaa ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag
      481 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
      541 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      601 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      661 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      721 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      781 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      841 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      901 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      961 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
     1021 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
     1081 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
     1141 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
     1201 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
     1261 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
     1321 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
     1381 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
     1441 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1501 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1561 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1621 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1681 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1741 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1801 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1861 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1921 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1981 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     2041 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     2101 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     2161 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     2221 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     2281 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     2341 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     2401 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     2461 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2521 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2581 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2641 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2701 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2761 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2821 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2881 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2941 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     3001 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     3061 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     3121 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     3181 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     3241 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     3301 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     3361 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     3421 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3481 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3541 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3601 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3661 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg
     3721 tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc
     3781 aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat
     3841 tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg
     3901 gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga
     3961 tttcgaaccg cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc
     4021 agcaaactat ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt
     4081 gcataaaaga agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct
     4141 gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa
     4201 gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta
     4261 tttataggga aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt
     4321 ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg
     4381 attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg
     4441 caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc tttcgga
//
LOCUS       DACIRY010000168         4426 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.175, whole genome shotgun sequence.
ACCESSION   DACIRY010000168 DACIRY010000000
VERSION     DACIRY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.175"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(279..809)
                     /locus_tag="IYE22_002569"
     CDS             complement(279..809)
                     /locus_tag="IYE22_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ9507750.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            914..1465
                     /locus_tag="IYE22_002570"
     CDS             914..1465
                     /locus_tag="IYE22_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9507751.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1618..2091)
                     /locus_tag="IYE22_002571"
     CDS             complement(1618..2091)
                     /locus_tag="IYE22_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9507752.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2229..3701
                     /locus_tag="IYE22_002572"
     CDS             2229..3701
                     /locus_tag="IYE22_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ9507753.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3698..4408
                     /locus_tag="IYE22_002573"
     CDS             3698..4408
                     /locus_tag="IYE22_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ9507754.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1390 a          807 c          851 g         1378 t
ORIGIN      
        1 caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct tctcggtaat
       61 aagcccacta gcctagaaaa tatgaaaagc tattttctaa ctagcgttct tattactcaa
      121 agcataacgc tgatttcaca acctcttgat aaacggtgtt caatcagctg accctcggta
      181 ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc gtttctttct ctaatactca
      241 ggtcagaacg ctgatttcac aacctctctt tcacaacctt attttactcg tttataattt
      301 tcatcgaagt attttccgct acggatatca tctggcattc catcgtatgg ttcttcgcta
      361 ataacatctt cattgtctat acctgcatca cccatataag tgatcgtagc aaattcagga
      421 acaagtaatt ttggataagt ttcatatttt tcacgagctg cttccatttc tgcgatatgt
      481 tcgttttgga aagtaacagt gatttctgga tgttcttcaa cccatttagg gaaaaagatc
      541 attccgtgaa gtttcttttc atttggcata aagtccaatg ccacgtctgt ccctgttggc
      601 tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct
      661 tttacccaac ggcctttgac cattccgcca tggatacggt aatcaacagt atggtcattt
      721 ttggcatacc attcatattc ccaaccattg tcatacgtat agataaagtg agttccaata
      781 aaatcttcca attctttaaa ttctttcatg tttaagcatc tccttataaa ttaaaacatg
      841 taataaacta catgttttcg attactcatt tatgatagaa tggatttaaa gatttgtcaa
      901 ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat attttgttag ggttattaag
      961 taagaagaag atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt
     1021 ttggcaagca aaacatagtc agatttatcc tgaacttgca aaaatggaag agcaaggaat
     1081 cattcagcat caagtagaga tcacaggtga aaaattggaa aagaaagttt atgaattaac
     1141 agaagaaggg aaggaacaat tggcagagtg gattcataca cccaccaatg aactaccggt
     1201 taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt
     1261 agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc atctattaga
     1321 gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg gacactacct
     1381 gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa cagatgtatt
     1441 agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga attcctttct
     1501 atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg ccttttttca
     1561 agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta gtggcgttta
     1621 gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga gcttgtaagt
     1681 taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt ctttgaagtt
     1741 accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga ttgcttttga
     1801 aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt ctgcagcttt
     1861 tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta tgactgtagg
     1921 taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga atcccttttg
     1981 ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt cactgatgcc
     2041 aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca tttttgtcac
     2101 tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt caagtaggtt
     2161 gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa
     2221 ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat aatcggacac
     2281 ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag accttattga
     2341 cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat gtgcaatggt
     2401 taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca tggctgatca
     2461 ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg atcggaacga
     2521 ttacttgctt tacagcacct aattttagta cattactaat cggtcgtttg atccaagctt
     2581 gcggtgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt ttcccgccag
     2641 aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct cctgctttag
     2701 gccctacatt atctggttat atcattgata actaccattg gcgtgacttg tttggtatgg
     2761 tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa agtgttattc
     2821 cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt ggattcggta
     2881 gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg aaagtaattg
     2941 gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa ttacatttgg
     3001 aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt gcagctgttt
     3061 tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca ttgtatattc
     3121 aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg ggtgcattga
     3181 tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc gcgaaacgtt
     3241 tagccatcac agggatgttc attttgacag cggcaactgc accatttgct ttcttgacaa
     3301 aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt gggatctcca
     3361 tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac ttgatcagtc
     3421 atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt acagctattt
     3481 tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct gtattggata
     3541 atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt taccatgccg
     3601 cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt ttattgaaga
     3661 aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag ttttgattgt
     3721 cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc aaaccttttt
     3781 atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc
     3841 caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc
     3901 tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa aagaaaaagt
     3961 gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta cggataacga
     4021 aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg
     4081 ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca atcacgaata
     4141 tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa gtacggacca
     4201 agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa
     4261 agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc
     4321 agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag cattcgcaaa
     4381 attagtgaaa gaagcaaaag gcgaataata ttttgaacac catgta
//
LOCUS       DACIRY010000169         4305 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.176, whole genome shotgun sequence.
ACCESSION   DACIRY010000169 DACIRY010000000
VERSION     DACIRY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.176"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            148..1137
                     /locus_tag="IYE22_002574"
     CDS             148..1137
                     /locus_tag="IYE22_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ9507755.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IYE22_002575"
     CDS             1134..1553
                     /locus_tag="IYE22_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507756.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IYE22_002576"
     CDS             1562..1870
                     /locus_tag="IYE22_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507757.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IYE22_002577"
     CDS             1874..2236
                     /locus_tag="IYE22_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507758.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IYE22_002578"
     CDS             2458..3864
                     /locus_tag="IYE22_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ9507759.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DACIRY010000170         4255 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.177, whole genome shotgun sequence.
ACCESSION   DACIRY010000170 DACIRY010000000
VERSION     DACIRY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.177"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            832..1755
                     /locus_tag="IYE22_002579"
     CDS             832..1755
                     /locus_tag="IYE22_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ9507760.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1771..2310
                     /locus_tag="IYE22_002580"
     CDS             1771..2310
                     /locus_tag="IYE22_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ9507761.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3574..4008
                     /locus_tag="IYE22_002581"
     CDS             3574..4008
                     /locus_tag="IYE22_002581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507762.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1610 a          638 c          700 g         1307 t
ORIGIN      
        1 ttatgagcaa attaatgcaa aatttggcta gcaaaaaagc taaaaaatat atgctaataa
       61 tcgagcgaaa taaacacttt ttcaagtgag cgaatccgcc atgacaaagc tgaagcagtt
      121 tatttcagct ttgtcatggc gtgtgccgac ttttttaaaa gtctaggcac acgagcaaat
      181 atctttatca agctctaatt tgcgttttaa gacgtttgaa acgcctaagt atataaatat
      241 ataagaatag gtttaagagt gcttaaatcg aattttagag ggcatttaga caattttagg
      301 gttgttaggg cttgccctga ccgcctgtaa ggcgttttaa gaacaagata agggatttat
      361 actaggactg tcttaaaatg tcttaaacga acgctcagag cttctcaaag ggttccttat
      421 gaacggcttt tagcaaagga gccggaggcg actgcgcagt ataatgtgag agcagagcga
      481 tcacaccttt ttagcgaacc gtagggagca aggtttggaa ttggcagttt tgataaattc
      541 gcgaacctgt ttagagaaaa aaggagtgat tttttaggcg ggaaaattag cctcccctct
      601 ttttatatct ctttttatat ctctttttat atctctttta tgtcggctgt gtccttactc
      661 tcccaagcaa ttcagagttt tttcaagtag gttttctacg cttttcaagt aggttttcta
      721 cgcttttcaa gtaggttttc tacgcttttc aagtaggttt tccgacatta ttgagtattt
      781 ttataaagta tgttactttt atttcatact ttataaaagt aggagaagct tatggcaaat
      841 gaaattgtaa aatatgaaaa tagattaaat catattcctc tgagaaaatt taatagtcgt
      901 gaaatgaact tatttttttc aattgcttca cgtgtacgtg aaaaaggtgt agatgagatt
      961 attttttcat ttcacgattt gaaaaaacta agcggttata cagaacgtgg ggaacaattt
     1021 ataaaagatc ttagtagcac gtatgacaaa ttattaagta ttaatgcttg gaatgatgat
     1081 ggaaatactt taactaaatt tgttgctttc acaaaatata gcattatacg agataaacaa
     1141 cttgttataa tttcagttaa tccagaattt aaaggacttt ttaatcaatt aaagaactgg
     1201 acaagattta atctagagca atttactaat ttggatagca catattcaaa aactatgttt
     1261 cgattattaa aacaatatcg aactcaaggc tggactgaat tttccaaaga aaaatttttt
     1321 gatttactag atattcctaa aagttattgg aatagtccgt caaatattga caaatttatc
     1381 ttaaggccaa ttaaagaaga actcactccc ttatttaaag gattaactat tcgtaaaaaa
     1441 tatggtaaag gtcgaggtaa acctgttatc ggttatcgtt ttacttggaa agctgaaatt
     1501 aaccatgctg acgatttttc caaagggaaa caagaagatt tacgtataaa attatttaat
     1561 attgatcaca atggagaatt aacacgagaa gaaaaatggc gcgctaaaga tcgaatacta
     1621 aatcttccct taggcactca tgaagctgat tttaaacaac aacaacaaac tgaagaaaaa
     1681 caggcagaaa aacgagaact ttcaaacaaa ctcaaacaag atttactagg aaatttacaa
     1741 aacttattcg attaagctta aggagtattt atggcagata aaacaatcaa agaacttgcg
     1801 gaagaattag gagttagtaa aacagcaatt aacaaaaaag tttctgacaa aaatcgaaaa
     1861 ctttggtttg caaaaaacgg aaacaagttt gtgattaacg aaaccggtca aaaagctata
     1921 aaatcaatgt ttttagtcga taacgcaaac ttaaatcgaa aactagttgg cgaaaaatca
     1981 aaaaccaaaa accataaaaa caccaaccaa gtttccgaat taaaactcct ttctttccta
     2041 gaaaaacagt taaatcgaaa agatatacag ctacaagaaa aagataatca aataaaacaa
     2101 atgcaaaaat tactcgacca gcaacaacaa ctcactctac aagcgaacca acaaattgag
     2161 aacctacaag aacagctcca tttaacttat acagaagaaa ctccaacaac cgaatcaact
     2221 gccttttctg aaaaaaaagc agacaataca caaccactta ttgaaaaaaa gtggtggcaa
     2281 atttggaaat ctaatagaac caatctctaa aattttctag aagttagttc ggatagtaga
     2341 ggaaaaagtt gtactacctg atggggagat cactcaaatt tttatgagaa agaaaaaata
     2401 gtttacaact gtaaacaatc aatgtttata gttgtaaact atcaaactat atggtgactt
     2461 attgcttaaa ataggttgaa atctcactca atttttggtg gaaaaacaaa ataaattcta
     2521 atcgattttg ttgaaaaagt cataaaaaaa caaatgtgat tttaacaaaa tttcaaaaaa
     2581 atcgtttgaa atttcgttta ttttttttga gaattttgat catcgtaggg tcattgtaga
     2641 atcattatga gatcatcgtg ggatcatcga ataaagtatg ggaaaggctt gcggtgcttg
     2701 gtattaaggg cattctccgc ctcccaggtt cttgaccatt ttctctcaac gtggtaaaaa
     2761 tcccacttat aagagaaaat ggtcaagaac ctgggaggcg gagaatgccc ttaataccaa
     2821 gcaccacaag caaaaaaagt tagagtaact tccaaaaaag aaaaattttt tagaacaact
     2881 tttgaaaatt taattttgat ttatatccgt tcaatttaaa catttgataa actgaaggct
     2941 agtatttaaa atgtgtatac aagtggtagc gttaaaaaaa gtggaggtat aacctatgtt
     3001 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
     3061 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
     3121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
     3181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
     3241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
     3301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
     3361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
     3421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
     3481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
     3541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
     3601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
     3661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
     3721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
     3781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
     3841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
     3901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
     3961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     4021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     4081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     4141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     4201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agcca
//
LOCUS       DACIRY010000171         4207 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.178, whole genome shotgun sequence.
ACCESSION   DACIRY010000171 DACIRY010000000
VERSION     DACIRY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.178"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..625
                     /locus_tag="IYE22_002582"
     CDS             <1..625
                     /locus_tag="IYE22_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ9507763.1"
                     /translation="GCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKI
                     FLEEVITVRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLT
                     LAAWLRYLIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKE
                     IFGVDLYQAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            616..1698
                     /gene="uxuA"
                     /locus_tag="IYE22_002583"
     CDS             616..1698
                     /gene="uxuA"
                     /locus_tag="IYE22_002583"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ9507764.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(1806..3359)
                     /locus_tag="IYE22_002584"
     CDS             complement(1806..3359)
                     /locus_tag="IYE22_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9507765.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            3507..>4207
                     /locus_tag="IYE22_002585"
     CDS             3507..>4207
                     /locus_tag="IYE22_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9507766.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDV"
BASE COUNT         1434 a          732 c          793 g         1248 t
ORIGIN      
        1 gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag atttatcgct
       61 gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc caggtatcat
      121 tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc caaatattcc
      181 tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc gttatggtga
      241 gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg tattcatacc
      301 attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag gaaaaccatt
      361 catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg atattcaact
      421 gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt caaataaaga
      481 aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag aaatgttcat
      541 caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact tacaagaaca
      601 tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga agaaaagatt
      661 accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac attgatggat
      721 ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga agaaattgag
      781 tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt
      841 ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg aaacttggca
      901 gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg ggtgaaatct
      961 gaccttgact accgcttaga agacggctca tccacgttag cttttatatc tgatgatatt
     1021 ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga atttgaattg
     1081 ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc ctatgcatca
     1141 gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat catcccaaca
     1201 tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata ctcgattttt
     1261 ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg catagtagac
     1321 agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc agaaaatgat
     1381 gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc acatgttcga
     1441 aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc cgaatacggt
     1501 tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa cgaatacatc
     1561 cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata cggcttatat
     1621 gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga aaaaggaaac
     1681 aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa gaaggatgtt
     1741 caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta tgtatcttat
     1801 ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc ttcttctttt
     1861 aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc tggtgtgatg
     1921 attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa agggcagtcg
     1981 ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga ttcatttgaa
     2041 aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg cgtatagttc
     2101 acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac taggtcttca
     2161 accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg attttgttct
     2221 ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac gctatgttct
     2281 tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag aaatccatta
     2341 taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc aggtataaac
     2401 aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac atgaggaacg
     2461 tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc gattttcgtg
     2521 atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat tggcagatta
     2581 ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata ctctgtgaat
     2641 tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat ttttaataga
     2701 aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt tttaaaaaaa
     2761 gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt agcagtactt
     2821 gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc ttttccagaa
     2881 aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc taaaaatgca
     2941 ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg ctgacttaaa
     3001 ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata actgactgat
     3061 tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat tccttcaaaa
     3121 ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt gagacgctca
     3181 aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat atggaaatga
     3241 tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag aaaaatatct
     3301 aattttgtat tgcttttttt cttcaagaga aacgttttaa tcgcttcttg agtttgcatg
     3361 aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt tttttcataa
     3421 atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat ttcttgcata
     3481 caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata tcaacatttg
     3541 cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag atagaaacag
     3601 ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag gatgaatttt
     3661 catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa
     3721 aaattgacta ttcaggagac ttagcaccta tactacaatc agctgttaca tttgagaatg
     3781 ttgatcccgc tttgtcgatt ggctatgacg agaacacttt gactatccaa cacaagcaag
     3841 cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg ctttttactg
     3901 tcaaggtatc agatggacta ccgaacgatc atcatcattt aacgccgtat agtcagcggc
     3961 agacaattga gcaagattca gctatcgact caggaggaga tcttgttgaa gaaccaacag
     4021 ataaaccaga aaatgaaaat aagccagaag taccgcctac agaaaatcca gacggagaac
     4081 agaaaccgga aatagaacct ggagaagaac ccgatacaga aactcaacca gagccagaca
     4141 atgaatcaaa acctgaaata acccctggag aaaaaccaga tgtagaccca gaagaaaaac
     4201 cagatgt
//
LOCUS       DACIRY010000172         4203 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.179, whole genome shotgun sequence.
ACCESSION   DACIRY010000172 DACIRY010000000
VERSION     DACIRY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.179"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            3..383
                     /locus_tag="IYE22_002586"
     CDS             3..383
                     /locus_tag="IYE22_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ9507767.1"
                     /translation="MIKKSKTMASYLEHKQSMEESAKVCQLKEAFLSKKEAFENVAAY
                     GEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQSLLDQIVQVLAEEVSNEIKV
                     DAGSPFFETKKHFGCGGNCHAIRQ"
     gene            367..705
                     /locus_tag="IYE22_002587"
     CDS             367..705
                     /locus_tag="IYE22_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ9507768.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            824..1384
                     /gene="rsmD"
                     /locus_tag="IYE22_002588"
     CDS             824..1384
                     /gene="rsmD"
                     /locus_tag="IYE22_002588"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ9507769.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            1381..1872
                     /gene="coaD"
                     /locus_tag="IYE22_002589"
     CDS             1381..1872
                     /gene="coaD"
                     /locus_tag="IYE22_002589"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ9507770.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            1862..2905
                     /locus_tag="IYE22_002590"
     CDS             1862..2905
                     /locus_tag="IYE22_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ9507771.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            3064..3792
                     /locus_tag="IYE22_002591"
     CDS             3064..3792
                     /locus_tag="IYE22_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ9507772.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            3908..4153
                     /locus_tag="IYE22_002592"
     CDS             3908..4153
                     /locus_tag="IYE22_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ9507773.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
BASE COUNT         1540 a          685 c          887 g         1091 t
ORIGIN      
        1 aaatgataaa aaagagtaaa acgatggctt cttatcttga acataaacag tcaatggaag
       61 aatctgcaaa agtttgccag ttgaaagaag cgttcttgtc taagaaagaa gcatttgaaa
      121 atgtagcggc atatggtgag tatgcacctg attatcgtga aaaaaaacga gctgttcgaa
      181 aagcaaaaag acagcttgat atggatgagc atgttgcagc tttccgatat agtgaaacac
      241 agctgcaaag tctgttggat caaattgtcc aagtacttgc cgaagaagtt tctaatgaaa
      301 tcaaagtaga tgcaggtagc ccgttttttg aaacgaaaaa acactttgga tgcggaggta
      361 attgtcatgc aattagacaa tagtgaattt gaaataacaa aacgacgcgg gctgatcgta
      421 tgggtctaca gcttaaaaca attaaagaat ctgaaaaagt tcggcttgtt gcattatgtc
      481 tcaagaagaa tgaaatatgt cgtgctttac ttaaatgaag agacattgga agaaacagaa
      541 gaacgaatcc gtaaattaca ttttgttcga aacgtagaac gttcttaccg accggatatc
      601 gaaatgaatt ttgccgaaaa aataggaaac aaaacagtat taaaagacga aggtttcgaa
      661 attgaagagc taaatacaga aatccgatta gctgaacatc tttaaaaatc tcttatgagc
      721 agtttttaaa taaaaaataa cttggataac gatcgatttt tgatcattgt ccaagtattt
      781 ttttggtata ctagtaccag taccatttct aaggagtgag tagatgcgga taatatcagg
      841 aaattatggc gggcgccgtt taaaaagttt agcaggggcg aacaccagac caactacaga
      901 taaagtaaaa gaatccatat tcaatatgat tggaccatat tttgatggag agacagtatt
      961 ggatctgttt gcaggaagcg gtggtttagc tatcgaagca gtatctagag gctgcagtca
     1021 tgcggtatgc gtagacaaaa attaccaagc gctaaaaatc atcaaagaaa acatcgaaat
     1081 tacgaaagaa ccagagaaat ttaccacaat aaaacttgat gcggacaaag caatcgaacg
     1141 gctagcagcg gagaaacaac aattcgacta cttgtttctt gatccgccat atgcaaaaca
     1201 aaaaataatc gatcaaatcc ataaaatgga agaactggga cttttttcta aaaccgcagt
     1261 tatcgtatgc gaaacggata aaacagtcga attgccagaa actgtggctg gattccatca
     1321 aataagaaaa cagatttatg gcataacggc cgtaactatc tatagaaaag agggaaatca
     1381 atgaaacgtg cattattccc aggaagtttt gatcctttta caaaaggtca cttagatacg
     1441 gtggaacgtg cggctaaatt attcgatgag gtagtaattg gtgtttttat caatacttca
     1501 aagaaaagtt tgtttccccc agaagaaaga atgacattga tcacaaaagc tgtttctcat
     1561 cttcctaatg taaaagtcat gcatcaagaa aatcaattga cggtagaaac agcaaaagag
     1621 attggtgcag atgcgttgat cagaggcata cgaagtatca aagactttga atatgaacga
     1681 gagattgctc agatgaatca tcatctatat gatgaattgg aaactgtctt cttattagca
     1741 aaaccagaat atagtcacgt gagttccagt attttaaaag aagtactgca ctttggcgga
     1801 gatgtgagca gctacttacc gccagtgatc aacgaagctt tagctggaaa gcgtgaatca
     1861 aatgaaagct aaacaatggt tgaaaacagt actgattatc atatttggaa tgttgatcat
     1921 tggatttgta gtagttccct tgccgtatta tgtagaatca ccgggagcca cgatcgatct
     1981 gaaagaacta attacggtca atgataagga agataaaggt tctgggtctt tctctcttac
     2041 ctctgttggt atcaggcaag ctacaggttt cttagcttta aaagctaaaa tgaccccttt
     2101 tgaagaattg atcagtgaag aagaattgac tggtggggcg acaaatgaag aatataatca
     2161 aatgcaacag tattatatgg attcttccca aaatgcagca atcgaacaag cgttaaaact
     2221 tgcagacaag ccttataaaa tggaattcaa aggcgtatat gtcatgagtg tagagaaaac
     2281 atcgaatttc tttggcaagc tttcagttgg agatacagta acaaaagtag acgggaaaag
     2341 ctttcaaagt acagaagcat ttatggatta tgtaaaaagc caaaaagttg gacaaacaat
     2401 cgaaatcagt tatttgcata atggtgaaga aaaaacagct tctggtgatc tgatcgagtt
     2461 accaacagat aaaaaggcag gaataggtat cacgcttact gaccataccg agatcgaatc
     2521 agatacagat gttcgtatta attctggctc tatcggtggg ccatcagcgg gattgatgtt
     2581 cactttagaa atctatgaac aagtcactgg taaaaacttg cgacatggta agcaaattgc
     2641 aggaactgga acgatcaaca gtgaaggaga agttggccga atcggaggaa tcgataaaaa
     2701 agtggcaagt gcggataaag ctggcgtaga gattttcttt gcaccagatg atacgatttc
     2761 agacgatgtt aagaaagaat atcctgaaat aaaaaccaac tacgaagaag caaaagctgc
     2821 cgctaaaaaa ttgaattcct caatgaaaat cgtaccagta aaaacagtac aagaagcgct
     2881 tgactatcta gagaaaatga aatgaaacta aataattaaa gaggggttgt gacacgagtt
     2941 ctgtcacaac ccctcttttt cgtcaaaaat cttcacgata aaaataaaaa caaaagaaaa
     3001 aattgacgta attagatgtt gaagggacga ttttgccaga gctcactgtt tcttcaattt
     3061 taaatgaaag gagtaatgaa tgatttttac tggtcattct ttgaaaaaag aacaatgata
     3121 gaaaaaatca gcaagaaaat actagcttca cccaaattga tcatttggat gtctatgata
     3181 gttcttatct tttgtatcgc aagtggtttt ttattatcaa atttgctatt ttatagagaa
     3241 acagaagaga ctgctgcaaa agaaatactt tcttcgttta gttccagtgg agaagatcca
     3301 tcagctgaag gaaacgatgt agaagaggat gctcgtccaa aggtcatgta caccgatatc
     3361 aaaggaagtg tgaaggagcc tggtatctat tccttttctt cagaagaacg ggtctatgat
     3421 gtactaaagc gtgcaggtgg attattagaa gaagcggata gcgatcgaat caacttttct
     3481 gcgaagatag aagatcagca agtcctatat atccctgctg taggtgaaga gctgccagaa
     3541 catcttgatc agttggcatc ctcggaagga aaacagccta cagcagattc cgagcctccc
     3601 aaaataaata tcaatacagc cactccatca gaacttcagc agattcctgg gatcggatca
     3661 gtcaaagcgc aagaaatcat tcgatttaga gaagaaaacg gatccttcca gaaagtggaa
     3721 gacctgcagg agatttctgg tatcggtgaa aagacagttg aaaaactaaa aaacttcgtt
     3781 acaattaaat agaatgattc aaaatcgact ggcacgagca ataaatggac gctaatggat
     3841 actaagaata atccaattct aagagcttgg caggacgagg aggtagtatt tttggatagg
     3901 aaatagcttg attgttcgca aagaaacatt aaaaaaacca atgctgaatg tctatctcca
     3961 aaataaaata tctgggattc atattatgaa tacggctgtc agcggcaata acagtcaagc
     4021 tctacgagaa cgctttgcca aagatgtact tagctataca gcagataaag tatttattct
     4081 cattgggacg aatgatctag cagagcataa acaactttct aaagaaacgt accaaaaaat
     4141 ctgcagtgga tgatagttca gctgaaaaaa gactatagtt ctgatcatgc tttctttatc
     4201 acg
//
LOCUS       DACIRY010000173         4157 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.180, whole genome shotgun sequence.
ACCESSION   DACIRY010000173 DACIRY010000000
VERSION     DACIRY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.180"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            5..89
                     /locus_tag="IYE22_002593"
     tRNA            5..89
                     /locus_tag="IYE22_002593"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Leu,seq:caa)
     gene            344..1219
                     /locus_tag="IYE22_002594"
     CDS             344..1219
                     /locus_tag="IYE22_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9507774.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1323..2087
                     /locus_tag="IYE22_002595"
     CDS             1323..2087
                     /locus_tag="IYE22_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ9507775.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2104..3516
                     /locus_tag="IYE22_002596"
     CDS             2104..3516
                     /locus_tag="IYE22_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9507776.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            3533..>4157
                     /locus_tag="IYE22_002597"
     CDS             3533..>4157
                     /locus_tag="IYE22_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ9507777.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1355 a          620 c          887 g         1295 t
ORIGIN      
        1 atttgccggc gtggcggaat tggcagacgc gctggactca aaatccagtg tccgttgagg
       61 acgtgccggt tcgaccccgg ccgccggtat aattatcaat atagcaactt tcttatagag
      121 agttgctata ttttttttgt ttttatgtaa ttacctccaa atcaattcga tacaagtatt
      181 atcataaaac gagtaaaaaa gatattgata acgcttttat tatgtgttaa actatttttg
      241 taaattatgt agggtgctac tattcaatta ggcacgacct aagaaagggt tctttcttag
      301 gtcgtttttt gtctttaaaa attgtttgga gacaaaaata tgtatgaagg agtctgcaag
      361 ttgttttgtt ttatcatata gaggaggtga attaacggtg tatgtgatga aaaagctgaa
      421 tcataatgct gtattgatcg atgacgatgg aatagaaaaa attgcgatgg ggaaagggat
      481 tggcttttct gctaaagtga gtaagccatt tgatgttgag gatgctgaca aattatttgt
      541 attagattct aaagaaaaga tcaaacgatt cagtgagata gtctcgcaga ttccaatcga
      601 atttatggat tttgcggaag aagtgattga actgataaca gctaggatct cctctccatt
      661 agattcaaat atctatatta cattgacaga tcatatttat tttgctattc aacgaaaaag
      721 agaagataga gatgtcacag caattatgtt gccagaaatg agattccttt atccaaatga
      781 atatgatata gcagtagaag tggtcgagcg aatcaatcat cgatatcaaa ccgagttagg
      841 gtcaaatgaa gtaggattca tcactatgca tatcattaat gctgagctag gagagcctaa
      901 tagtttaaag agcttaaaaa tagtagaaat gacgaccacg attttgtccc atctagaaaa
      961 atcctattca aacgtttatg ataaagaatc actgacttat catcgcttaa tggttcatat
     1021 caaattcctg gttaaacgct tgatttatca ggaagaaacg ccagtagaaa atattggttt
     1081 ttttactgaa acatttaaac atagcaaagc gtattgtaca gcactaaaga ttaaagatat
     1141 catcgaacag caatttcaac ttgccattca agaaaatgaa atcatctatt tggcaattca
     1201 tctagcaaga atctattaaa ataagggcgt aactatctat aggcgcaacc aaatgacaca
     1261 tagtaaatgg actatgatta tttggttgcg cctattttag tagaaaaaag aaggagaatg
     1321 aaatgggaaa agtgattttt aattcggatg attttggcta tagccatggt gtgaattatg
     1381 ggattatgga tgcttatcag cgaggaattt tgacatcaac tacattgatg gcaaatatgc
     1441 cgggtttcga gcatgctgtg aaactaagaa aggaaatgcc taggctaggt gtaggagtcc
     1501 atttaacatt gacgtgtggc aaaccactcc tcaaaactgt cgatactctt atcgaaagcg
     1561 gagagttcag atccttatct tattatcagc atccatttaa cattgacaaa gatcaactgt
     1621 atcaggaatg gaatgctcag attcaaaaaa tttatcatgc ggggatcata ccaactcatt
     1681 tagacagtca tcatcatact catactttgg atgaaaatca ggaggtagtt gttacattag
     1741 caaaaaaata tgatttgccg gttcggagca actttgaacg aaaagatgaa gttcgtcatg
     1801 tcgattattt tgagccttgt ttcgatgtcg taggagaaac tgatgaaaaa aaacgacaaa
     1861 tcaatacgag tgtggaagaa tatttggcaa gtttactcat gatattgaga gaagataaaa
     1921 cgatcgaagt catgtgtcac gcagcgtatt tggatcagga attgttaaat ggttcaagtt
     1981 ttgtgtatcc tcgtacccac caagtcgaat ttttaattca ttcagcattt gctcaaaaag
     2041 tgaaaaatga tcctttgatt gagttagtaa catatgagga tatttaatag aggaggaata
     2101 aaaatgaatg ttaaatcaaa tgtgcagaag cttggaagag cgatgctact tccagttgca
     2161 gcgatgccat tagcgggatt gatcatgcgt ttatcagctg acgatatgct gaacatccca
     2221 gtaattggtg cagctggaaa tgccgttttt ggaaacttgg atatcttatt tgctatagga
     2281 gttgcaatcg gttttgtcaa aaataaggac aaaggaatac ctgcacttac tggtttttta
     2341 gcgattgcta ccttgaaaaa aggcttagaa attatggacc cagaagtgaa tatggggatt
     2401 tttggtggaa ttatttccgg tttagtagcg gcatggacgt ataatcgctt taaagaacaa
     2461 aaattaccag tagttttttc ttattttgcg ggcgaaaaat tcccattgac tatggtgatg
     2521 attctacaaa caatcgcagc tgtttttttg ggctttatct ggccgctcat ccaaattggg
     2581 attgataatt ttgcaaaact gctcgttgat atgggagcat taggtgtggg gatatttatg
     2641 tttttgaatc gtttgctgat cccctttgga ctgcaccatg tgttgaatac gtatatttat
     2701 tacgatttag gaagctatac atctcctagc aaggaagttt tcagaggaga gatgacacgc
     2761 tttatcaacg gagatcctca ggctggactg tttttatcag gtttttttgt agtaatgatg
     2821 tttggtatac cagctatatg tctagctatc tataaagcag cttataaaga aaataaagag
     2881 atggtcaaag gaatcatggg aagcggtgca ggaacttcat ttattgcaaa tatcactgag
     2941 cctgttgaat ttagtttcat gtttctttct cctatgttgt acgtgatcca tgcacttttt
     3001 gccggtttag cagggattgt ctgttactta ttcaatatac gaattggttt tacattcgga
     3061 gcatgtattg ttgattatct catcaatttt cgaatcgcta ccaatgctat tttgattctt
     3121 ccaatcggaa ttttcttttt tgccttgtat tacgttactt tttattattt gatcaataag
     3181 agaaacattc agacattagg acgtgaagct aaggcagagt ttggcaatga agtaacctta
     3241 gaagaaacag agttagggtt agcaagtaaa aattatgaat atatggcaac aaaaatgcta
     3301 caagcgtttg gaggaaaagc caatatcctg gatgtctata gttgtaatac acgcctacgg
     3361 gtagaggtag tggatcccac catggtcgag gaacagcgaa tcaagcaatt agggataagc
     3421 gggatcatta aaccaactga aaaaaattat caaattatca tcggcttaga agtgacttat
     3481 gttatggcag aattcaataa attattagaa gaatagaaag gaaaagatga tgatgaaaaa
     3541 acagattatc actatagctg gaggcgggag tacgtataca ccaggaatcg ttcaagccat
     3601 tttgaacaat gaaaaacggt tgccattatc tgaaatacgt ttatatgata ttgatgaaga
     3661 aagaaatatg gatatgtact tgattgttaa atttatgctg aaaagaaaag gtttttcaaa
     3721 tattcgcatt cgtgcaacgg atgatccaaa gcttgctttt acaggatgcg attttgtttt
     3781 ctcacaaatt cgtgttggag gattggagat gcgagaaaag gacgaaaaga ttccacttaa
     3841 acatggatta gtaggacaag aaacgtgtgg actcggagga tttgcttatg ggatgcgctc
     3901 aatcaaaggt ttattggaaa ttgtcgatca tatacaagac tatgccccca aagcatggat
     3961 tttgaattat acaaatccag agtcgatcgt ttctgaagct gttcgtcgta aatatccgaa
     4021 tgtaaaaatg attaatgcct gtgatatgac tatttcaatt gaagaaacga ttgcagtgaa
     4081 ttacggttat gatcgtaaga attggatcgt tacgtattat ggtttaaacc actttggctg
     4141 gtatacatcg atttatg
//
LOCUS       DACIRY010000174         4139 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.181, whole genome shotgun sequence.
ACCESSION   DACIRY010000174 DACIRY010000000
VERSION     DACIRY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.181"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..2323)
                     /locus_tag="IYE22_002598"
     CDS             complement(<1..2323)
                     /locus_tag="IYE22_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ9507778.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDL"
     gene            2771..2992
                     /locus_tag="IYE22_002599"
     CDS             2771..2992
                     /locus_tag="IYE22_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9507779.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3100..3447)
                     /gene="rbfA"
                     /locus_tag="IYE22_002600"
     CDS             complement(3100..3447)
                     /gene="rbfA"
                     /locus_tag="IYE22_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ9507780.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3474..>4139)
                     /locus_tag="IYE22_002601"
     CDS             complement(3474..>4139)
                     /locus_tag="IYE22_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ9507781.1"
                     /translation="ATSRVTLDNLFESLKEGELKEVNVIIKADVQGSAEALAASLKKI
                     DVEGVRVKIVHSAVGAINESDVTLAAASNAIIIGFNVRPTPQAKVQADTEEVDIRLHR
                     IIYKAIEEIETAMKGMLDPEFEEKITGQMIVRETFKVSKVGTIAGAYVTEGYIRRDSG
                     VRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYECGAMIEKFNDIKVDDVIEGFIMEEV
                     KND"
BASE COUNT         1091 a          890 c          749 g         1409 t
ORIGIN      
        1 gcagatcaat accattttca cgaatatagt tttgatgttc ttgtaatttc tgatccattt
       61 tttccgcaaa atcttctgct tgacttcctt tgatttttaa agctgcgtct tttgccaaat
      121 gataacgatc aagttcattt actacacgca tgtcaaatgg tgtcgtgata tctccatttt
      181 ctttgtagcc atgaatcatt aaatgatggt tatgacgatc aaagaaaatc gtgcggacca
      241 atgtttcata accgtggaaa gcaaataaga tcggtttgtc ttttgtaaaa tattgatcaa
      301 attcttcatc gctcaaccca cgcggatctt gacttggatg acgtaatttc aataaatcta
      361 cgacattgat gaaccgaatt tttagttttg ggaattcttc cagcagcaaa gttacagctg
      421 ctaatgcttc taagttagat tccgttcctg cagcagcaat gaccaattca ggttcctcct
      481 cttttgctgt cgaagcccaa tcaatgattt tcaagccttc attcactaag acggctgctt
      541 cttctgctga gtaaaattga gcacgcggat gtttggaaga gatgatcaaa ttgatttttt
      601 ctgacgagcg gaatgcttta tccatgactg cgagcaatgt atttgcatcc gcaggcagat
      661 attcacggat gaactccgcc ttcttttctg ccaaatgtgt caatattcct ggatcttggt
      721 ggctatatcc attgtgatct tgctggaaga ctgttgagct tgcaatgaga ttcagcgaag
      781 gataatcttt tcgccaagaa aggtcatgtg atttccgtat ccatttgaag tgctgagtca
      841 acatagagtc aaccactcgc aagaatgatt cgtaacttgc aaaaaaacca tgccgtccag
      901 tcagaacgta cccttctaag aacccttcat cttggtgttc cgataattga ctgtcgatga
      961 cgcgaccaga tggcgaaagc cattcatcat tttctggttc gattttttcc atccattggc
     1021 gatttgtcgt tttgaaaact tgattcaatc ggtttgattt tgtttcatct ggtccaaata
     1081 tacggaaatt ttctggattt ttctcaatga tctcgcgaac gaatttacca agttcggtca
     1141 tatcttctgc tttaacggag cctggttttt caaattgcaa agtatatttt ttccaatctg
     1201 gcataataag cggtcttgga tcgattccac cattggtgat aggatttttt gccattcttt
     1261 ggtcaccagt aggcgcaatt tctctgattt cttctaaaat acgtccttgt gcatcaaata
     1321 atttttctgg ttcataggat ttcagccaac ggagtaaagc atctgcatga tccatatgtt
     1381 cttgatctac aggaatcggt acttggtgtg ctcggaaggt tccttcaatt ggttccccgt
     1441 cccattcttt tggaccggtc catccttttg gtgtgcgtac gatcaatact ggccattttg
     1501 gcatcacgac atctgttgct gtcttcaggc gagcttcttt ttggatggat ttgattttct
     1561 cgattgcttg atccattttt tcagccataa caggatttaa tttttctgga tcatttcctt
     1621 caataaagaa aggctcccag ccaagtccgt taaagtaatt tgctaattct ccatctgatt
     1681 tacgtgccaa gatcgttggg ttggctattt ttgcaccatt taaatgaagg atcggtaaaa
     1741 cagctccatc tatcactgga ttgataaaga catttgagaa ccagcttcca gctaaaggac
     1801 cggtttctgc ttcaccatca ccaatcacga cagcagagat tacttcagga ttgtctaata
     1861 cagctccgac tgcatgtgaa agagagtatc caagctctcc gccttcatgg atagaacctg
     1921 gtgtttctgg tgctgcatga gaagcaatgc ctccaggaaa tgaaaatcgc ttgaataatt
     1981 tttgcattcc tgtctcatct tccgttactt ctggatagat ctctgtgtaa cttccatcta
     2041 aataagcatt cgataccatg acttgtccgc cgtgtcctgg cccttcgata taaaacatgt
     2101 tcaaatcata tttattgatg actcggttca aatggacata aataaaattt tggccaggaa
     2161 tcgttcccca atgaccgatt ggatgttttt tgacatcttc tggtttcagt gtacgtctca
     2221 gtaaggggtt atctttaaga taaagttgcc caacagaaag ataattagca gcacgccacc
     2281 aagccgtcat tttatcaaag tattctttac tagaataatc catacataac actccttttc
     2341 attattactc ttattctagc gcagatactc ccaattgaaa cctaataagc acttctgttc
     2401 atttgtttgg attttatatc tagccaaatg aacaggtata aaaaaccacg aaatttttcc
     2461 tttcgctgac gttttttaca ttgatccaaa aaacttgtcg ttatcacaat ggctgttccg
     2521 cagttcattt cgctcctcat catgcataat ttgctgaatg acgctaggcc agttaacaca
     2581 ttgttggaaa actggggctg gatcgagcaa ttcctttcct gattgatccg ttttatgtaa
     2641 aagtctctgt tatctttgtc aatatgtgaa ttggtatccc agctactatg ctaatttcta
     2701 cagggattat ccaaaatttg gatcgtgacc aaatcgaagc ggcgtaatcg gaaatatcaa
     2761 taacttcaac gtgatttttc ttttaactgt caacggaccg acaaactcaa acttttatgg
     2821 tgcaggttcc actgatcttc tagttacttg gctatacctg actgtcacta aaatagatta
     2881 caatctagct tccgttatcg gtatcttgat ctttatctat atgcaaaaat ttatatacta
     2941 tagaaaaaag aaagtcgaat tttttacttt tctttttctc ttttttggtt agctgacacg
     3001 ctaccagact aaagtcatgg aactcttagg taatacgtct taatgaaaac aaaaaaatcc
     3061 cgcaacccct gatttcacag gatttgcggg attttttgat tagtctttgt tcaagtcgcg
     3121 aagcaattca tcgatatggt tcccatagac aactgattca tcaagttcaa agaacaattc
     3181 aggtgtctta taaatactta gattgtgccc taactctcga cgaatcaatc ctgatgcttt
     3241 attcaatcca tcttgtgctt ttttcttatc tgatgctaag tcagataaaa tgctgtaata
     3301 gatcgttgct tgctgtaagt cccccgtaac tttcacatct gtgatcgtta cgttctccac
     3361 acgagggtca cggacctttt tgcgcaaaat atcattgact tcttttaaga tttcttgacc
     3421 gactcggcga tcacggtagt tagccataat gattggcctc cttttatcag tttttagtca
     3481 tttttgactt cttccatgat aaatccttca attacgtcat ctactttgat gtcattgaat
     3541 ttttcaatca tcgcaccaca ttcataaccc attttgactt ctttcacgtc atctttgaat
     3601 cgtttcagac ttgcaagttg gccttcatag attacgattc cgtcacggat tacacggaca
     3661 ccgctatctc tacgaatata tccttctgtt acataggcac ctgcaatcgt accaacttta
     3721 gaaactttga aggtttcacg aacgatcatt tgaccggtga ttttttcttc aaattcaggg
     3781 tctagcatcc ctttcatcgc agtttcgatt tcttcgatgg ctttatagat gatacggtga
     3841 agacgaatat cgacttcttc agtatcagct tgcacttttg cctgcggtgt aggacgaacg
     3901 ttgaaaccga tgataatagc gttgctggct gctgctaagg ttacatcact ttcattgatt
     3961 gctccgacag ctgaatggac gatttttaca cggacacctt ccacatcaat cttcttcaat
     4021 gatgcagcca gtgcttctgc tgaaccttgt acatctgctt tgatgattac gttaacttct
     4081 ttcaattcgc cttctttcag gctttcaaat aagttgtcca aagtcacacg gcttgtggc
//
LOCUS       DACIRY010000175         4126 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.182, whole genome shotgun sequence.
ACCESSION   DACIRY010000175 DACIRY010000000
VERSION     DACIRY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.182"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            94..1005
                     /locus_tag="IYE22_002602"
     CDS             94..1005
                     /locus_tag="IYE22_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9507782.1"
                     /translation="MQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSK
                     LATMSQQLVQSDPDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGG
                     NVTGVSDQPPVTSQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESV
                     KYAVPSTNEIAQTVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMV
                     EQGGLATIGINQYQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGI
                     IVPQSMKEKAKIITDDSQQETSKEDDK"
     gene            1002..1883
                     /locus_tag="IYE22_002603"
     CDS             1002..1883
                     /locus_tag="IYE22_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9507783.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            1886..2656
                     /locus_tag="IYE22_002604"
     CDS             1886..2656
                     /locus_tag="IYE22_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9507784.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            2935..3789
                     /gene="aroE"
                     /locus_tag="IYE22_002605"
     CDS             2935..3789
                     /gene="aroE"
                     /locus_tag="IYE22_002605"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ9507785.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            3967..>4126
                     /locus_tag="IYE22_002606"
     CDS             3967..>4126
                     /locus_tag="IYE22_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ9507786.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFE"
BASE COUNT         1364 a          734 c          910 g         1118 t
ORIGIN      
        1 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
       61 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
      121 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
      181 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
      241 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
      301 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
      361 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
      421 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
      481 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
      541 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
      601 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
      661 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
      721 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
      781 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
      841 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
      901 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
      961 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     1021 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     1081 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     1141 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     1201 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     1261 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     1321 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     1381 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     1441 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     1501 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     1561 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     1621 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     1681 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     1741 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     1801 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     1861 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     1921 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     1981 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     2041 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     2101 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     2161 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     2221 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     2281 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     2341 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     2401 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     2461 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     2521 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     2581 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     2641 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     2701 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     2761 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     2821 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     2881 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     2941 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     3001 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     3061 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     3121 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     3181 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     3241 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     3301 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     3361 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     3421 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     3481 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     3541 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     3601 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     3661 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     3721 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     3781 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     3841 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     3901 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     3961 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     4021 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     4081 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaac
//
LOCUS       DACIRY010000176         4108 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.183, whole genome shotgun sequence.
ACCESSION   DACIRY010000176 DACIRY010000000
VERSION     DACIRY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.183"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(45..1022)
                     /locus_tag="IYE22_002607"
     CDS             complement(45..1022)
                     /locus_tag="IYE22_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ9507787.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="IYE22_002608"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="IYE22_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ9507788.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            complement(2595..4019)
                     /locus_tag="IYE22_002609"
     CDS             complement(2595..4019)
                     /locus_tag="IYE22_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ9507789.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         1126 a          872 c          720 g         1390 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt ctttaatcta
     2581 tttactttgt gaaattattc tgttaaccac ttttctagaa ttttcatctt tgaataaaac
     2641 aacggtattt ggttttcatc cagcctactg taattgattc tgattccatt tatatcttgc
     2701 ccaaaaagta gacttggtgc aattccaaga cccatatcta ctactttttc tgctagttcg
     2761 gggtctagtt ttttcccatc ccaagtaatc caggcatagt aaccacctaa aattgaatga
     2821 agcttgaaaa acgattgtga atctccccaa gcgcttatca gtcggctttt ttctttcaac
     2881 ttttttctta catcttgcat tttcaaatca attgcttcat taaatacatt agccactgtg
     2941 atttgtgtaa ggatattcat cgaaaactcc atcatttctt gcgcttgagc tagttgcgag
     3001 ataaaagtct caggtcctat cacccaaccg attttcgttg tttttcctaa aaccctagat
     3061 aacgaaccga cgtataacac attatccgga tcaatcgttc gtataggaag gacttcttct
     3121 tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa taattggaag ccgatactgt
     3181 cgacacaatt ctactagctt ttctcgtcgt ttttgactca tcacttttcc tgtagggttt
     3241 tgaaaattcg ggttgataag cactgctttc aatttatttt tatggatcga ttctttcagt
     3301 ttttctaagt cgatcccttc ttcatccatc ggtatcccaa ataaacgcgt tcctgaagct
     3361 ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta cagctatccc ctctccagct
     3421 gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac cgccaccagt cacaagaagc
     3481 tggttggggg aaaatgccaa ttcaaattta ctttccaaat accgtttgat tttgttacgt
     3541 aacggagtat agcctaaaag atccatttct ttttcttccg ctaagatttc ttcaagagaa
     3601 taagaaggaa actcaaaatt cggcaaaagc tcatctggca tctcgctagc gtataaatca
     3661 agaaacgatg ttttttggat ctgctttgct tctcttattt tttgctcata ccagtccgtc
     3721 tgctcataac gaggagagat catttgccgc caatcgattc ttggaacagc gaatttcccc
     3781 cattcagtct gattaacaaa ccatccgctg ccacgtttgc tcgttagtac cccaagctcc
     3841 ctaagctctt ccaatgcatg gacaacggtg gaacgattca caccaaaaag ttgccctagt
     3901 tttcgttctg attcaagttg acttccagga ttgatccttc cgttttcgat ttctctcata
     3961 atctgttcca taatttgacg ataaatcggt ccttgttttt tctctacttt ttcccacata
     4021 agcttctctt ttctattgtc tattttttct tttgatttta ccgaatttac gagaaaaaag
     4081 aaaaactttt tttgcaaaac ataaggga
//
LOCUS       DACIRY010000177         4058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.184, whole genome shotgun sequence.
ACCESSION   DACIRY010000177 DACIRY010000000
VERSION     DACIRY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.184"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..448)
                     /locus_tag="IYE22_002610"
     CDS             complement(<1..448)
                     /locus_tag="IYE22_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ9507790.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(755..1852)
                     /locus_tag="IYE22_002611"
     CDS             complement(755..1852)
                     /locus_tag="IYE22_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ9507791.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1960..3987)
                     /locus_tag="IYE22_002612"
     CDS             complement(1960..3987)
                     /locus_tag="IYE22_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ9507792.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
BASE COUNT         1161 a          803 c          633 g         1461 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aaccatccta
      721 caataataaa aatttactat ttgttatctt aaccttactt ttctttctct tctttttttc
      781 tcaaaacaaa caaaagtatg cataataaaa taaacaataa gaaaagtgaa gctactaaga
      841 tataaatcca attattgtct tttttaatcg taacatccgt cgtgttcaat tccttggcta
      901 cttttccgtc tattgtaaaa tctttttcaa atacccattg attggtatag ttgatcgtag
      961 tatttccttt ttttctcgaa aattttccat tttcattttc atttcctaaa acagtcattg
     1021 taaggtgata ttcacctggg gttagcggtt gaccatttaa agcagttgga aaagaaaaat
     1081 ttgtattcgg cgcaatctgc aatccttctt tttcctcttg atataaaact tcagaatgtc
     1141 ctttttttgt aatttttgtt tcaataacta cttgattgat ataggttttt tgatcatttt
     1201 gaacgtttgc taagatgaca tttcttgcat tgatttgatc tggtttaact tcatgaagag
     1261 taaggttcgg agcaacttcg ttcagtgtct gttgcatcaa caaagcaata atataggtgt
     1321 attcattctc aatcgataag ctttgatcct tagtttctgt ttggtccttt tccgtctgga
     1381 tttcttggaa tgtgatccca ccagccaata caccatcaaa tcgctcattc ggcatcctga
     1441 catgaaaagc aacggtttgg acacttttag gttttagcgt cactatctgt ggatagctca
     1501 cataatctac cagatcaaag atcaggctca tgtctttttc aatctcgttt tttccgtatt
     1561 caaccatcac atttgaattg gtggttgcgc tattcacaga aatatcgatt ttcacttctt
     1621 tttctgtatc atttctcaat tccgctttta cttctacttc ctgatccgga tcaagttgaa
     1681 gatcgaagta agtttttctc ttgtcgattt gattctctga agttatcggc gtcactgaaa
     1741 aattaaatgc actagctgac actgatatat tgttaaaaag tgcccctaaa ataactagga
     1801 gtgttataaa taattgttgt tttggtctca agaaaatcgc tcctttaatc atatgtaaga
     1861 gaagactata acaaaatctt gtctctgtgg gacacaagag cctatggtac ctgtgccctt
     1921 cttgaaactc aataattttg ttatattctt ctcttcctgt taatttcctg ggacatctgc
     1981 aagtatccaa gatagtttcc cctcaaaggt tccgattctt tggttttcta caggaatatc
     2041 tattctgatt ccacgatgag ctgtagaatc taaaagagag gacaaatcgc tttcaccctt
     2101 ttgatcactt tcgaaaatcg taacagcgga tgtccctata accgttgtat tttcagaggt
     2161 aacaaaggaa agagcttctg gaagctcaaa atctccattt gtaagaactt tctcttgttt
     2221 caattgtaat ttccaacttc caaaagcccg ttcatctata attttcaacc cacctgaaaa
     2281 ctgtccgaat tcacttaatg gtctactgga tattttatgt tttccaaaat tgacatattg
     2341 aggagcagtt gagagataaa gtttgccttt tctgtaataa aaatagatat cctctgtatt
     2401 ttcggagaag atgccttctg tattcgctga atccgagtca aaaacatacc cttcaatatc
     2461 aataggagtt tctttatact tctctcctat tttcccagtg attgttttca tttcagcaat
     2521 atcaatctga ctgtctttat ctttataata aacatggata tctatctcct tcagctttgc
     2581 gatctttagg tttatttcat tcgattgctc aatcaaattc ccgttttcaa ggtattcaaa
     2641 atgcatagtg agtacttttt caggaattac ttcctgcaaa gttgtctcca attcgattat
     2701 taacgtatct ccagcaataa aataatctgt cagatttagc gtgactcgat ttccggttat
     2761 caagatatct ttggcatcaa tttgatttcc attgatcaag atatgctcgg gaatagttaa
     2821 ttcttgtggg aggtcgatac tagtctgcat tttcttatat tcaagaaaat ccccctcgac
     2881 cttcaatgta tttctgatag ttatcttatc gtttcctcca gcttcagcga tggccccata
     2941 atcactatcg ttgactttta catcagctgt tgatgtgaca ttcgatggaa gtcgcgtgac
     3001 agcgacagca ttttcttgaa aaaaagtacc tgttgaagaa gtaaaccccc aatttacttc
     3061 tgtagaacca aactgcctaa tgagatcagg tataatccga gaagagtgac cgacaccttc
     3121 caaatcataa gtaagacttc tttgctccgc gtcccattta attattaaca agcgccatgt
     3181 gccattagaa agaaatgtag gagctaccgt cacgccagta tgctctccag aataattatt
     3241 attatttggt ttaggcgtga caaaagcgac atgtccatgc cctctattat cagcagttat
     3301 ctctcgatcc attcgatcag aagaaccact atttttatac gtatcaaact caatcgagag
     3361 cgcattcgca atgtaaggtt ggccagaacg agtagaatat gagccaattc ccatacctgg
     3421 cgatccaata acttgactgg gattactagc cattctagga tcgccagtca atgtaaaggt
     3481 catgccatct cccgctctat ctcgttgatt tcctaagtat aaataagatt taaatgtaaa
     3541 atccgagagt aaactaatct tcttatttga ccaaatagct cctctttgag acctttctgc
     3601 tgggttcaac tgcagtaact tcccattttc caatatggac gtgctagtac cgataggtgt
     3661 ttgaaaaatt ttatcgatag aaataatatt gggatcgtca ggcagctcaa ccgcagaaat
     3721 tggtgcatct tctagtcctt gtttgtccga accctcagct tctacgttgt cagtggtctc
     3781 tccactactc gaaatactta aatcctcact gctttcttgt gtctggttgg attctattaa
     3841 actgtcgaat gtcgaagtga gtttgctttc ttcgtctctt ctatttccat gctccaactg
     3901 attcaattcc aaagagtacc ctatgatcgg aaagatatta gtcactaaga gactgcttaa
     3961 aatgagcacg atcctcctct ttttcaatat atcttttcct cctcgtaaaa tatcatttgt
     4021 ctaatagttt ttattcaaaa tgtttctaga ttataaaa
//
LOCUS       DACIRY010000178         3994 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.185, whole genome shotgun sequence.
ACCESSION   DACIRY010000178 DACIRY010000000
VERSION     DACIRY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.185"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..377
                     /locus_tag="IYE22_002613"
     CDS             <1..377
                     /locus_tag="IYE22_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ9507793.1"
                     /translation="TAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            380..1093
                     /locus_tag="IYE22_002614"
     CDS             380..1093
                     /locus_tag="IYE22_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ9507794.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            1113..1631
                     /gene="msrA"
                     /locus_tag="IYE22_002615"
     CDS             1113..1631
                     /gene="msrA"
                     /locus_tag="IYE22_002615"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ9507795.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            1628..1855
                     /locus_tag="IYE22_002616"
     CDS             1628..1855
                     /locus_tag="IYE22_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ9507796.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            2007..2558
                     /gene="lepB"
                     /locus_tag="IYE22_002617"
     CDS             2007..2558
                     /gene="lepB"
                     /locus_tag="IYE22_002617"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ9507797.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2641..3183)
                     /locus_tag="IYE22_002618"
     CDS             complement(2641..3183)
                     /locus_tag="IYE22_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ9507798.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(3420..3818)
                     /locus_tag="IYE22_002619"
     CDS             complement(3420..3818)
                     /locus_tag="IYE22_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ9507799.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         1430 a          696 c          711 g         1157 t
ORIGIN      
        1 aaaccgcacc tatttacatt ttaggcatct acaatccatt ttatttgaac tttcctgaga
       61 tcactgacat gcagacaatt gttaacaatt ggaacaaagg aacagaaacg gtgacaaaaa
      121 aaatgccgaa ctgctacttt atcccaatca atgatttgct ttatcaagga ttgaatcagg
      181 aagtaggtgt cacagaagaa ggaacagatc aaacaactag cacgacagaa aatctaaatg
      241 gcatcaaaaa taatgtgctc tatgaagaag atcatttcca tccaaataat ttaggttatc
      301 aattgatggc aaatgcagtg agagatcagc tgatcgagac acaaaaccaa tggttagaga
      361 aggaggatga tcaataaaca tgaaaacaag atctgacgta tcttcaaaca aaacaaagaa
      421 aacaaattac tggaaattcg ccttccttat ccttcttggg gtagtacttg gttcgaccgt
      481 atttttaggc agccgtattt ttgctaatcg ggaaccagaa ctcccagaaa taccagctct
      541 tacggaaaga caaggagatc ctgtcttaac gatcaattca aataaagaaa aagtcaatca
      601 aatcatcagc tttttcctta gtgagtatca aaaagatagt gatattgaat ataaatttta
      661 tttggaaaat gaagcactac tcaatggtac tttcgaagta ctaggttttc ctatcgattt
      721 ctatttatat tttgatccgt atgtgatgga aaacggaaat gtccaactaa aagctagaag
      781 tctttctatc ggaacattga gtttgccaat caaagatgtg atgaatatga tcaagagaaa
      841 ctataaatta ccagattgga tcgaagtcaa tacagatgat ctgactatca tgcttaggtt
      901 ggatcaattc cgaatgcaaa acgggatgta tattaaagca gataaaatcg acttagtaaa
      961 tgatgacatt cggtttagtc tatatttgcc aaaagatacc tctgatgctt caaacaactc
     1021 aaataccaac aaaactagca ccagcagtac tgaaagcaag acaaaagaaa ataaaaaatc
     1081 atcggatgag tagtattaga gggggaatca taatggaacg agcaatattt gcaggaggct
     1141 gcttctggtg tatgatccag cctttcgaca cgcttcctgg tatccataca gtgatgtcag
     1201 gatatactgg agggcatgta ccagatccga cttatgaaca agtaaaaagc caaacgactg
     1261 gacatacaga agcagtggag attatctatg atccaacatt gatttcatat gaacaattag
     1321 tagaacttta ctggcagcaa actgatccaa ctgacgcttt cggacaattc gaggatcgcg
     1381 gtgacaatta tcgcccggtg atcttttatt tgacagaaga acagcggatc attgctgaaa
     1441 aaagtaagaa acagttagct gaaagtggac gtttcgaaca gccaatcgtt acaaaaatcg
     1501 aaccagccca accattttat ctagcagaag aagaccatca agagtattat aaaaagcatc
     1561 ctgaaaattt taaaagaaac catgcacgcc gggctgcctt tatcgaagaa aattggagga
     1621 acgaagggtg aatcgaagct tttttcatta tttaatgaca ctgcgaggcg gtggcacaca
     1681 tgatgcttta tctgttttcg ctacagaagc cggtaaagac atccaatttc ctaagcacag
     1741 ctctagctac gaagaaattt ctaattatct ggaactgaat gtcgactatt tgccgtccat
     1801 ggatatcttt gacgaagcat gggaaaaata tttagaaaat aatcatgaca tataagagat
     1861 agttttgtac tttatgatca aaaaagtttt ttgtccaata gcctaattga aagaattatt
     1921 tttatttcag ctatctagtc gcattttttt gatatttttc ttataataaa gaaagaatga
     1981 aaaaaagagg gatggattga tagagattga caaaaaaaca acgctatatt aatcgcttct
     2041 ggcttgtatt caaatactta cttgtttctg tattccttgc gttcatgttg cggggatttt
     2101 tattcatccc ggtaccagtg gaaggcaatt caatggaaaa cgtgttgaag caaggtgata
     2161 tggttgtgat ggaaaaattt tcggagatca gacgatttga tattgttgtt tttcaattag
     2221 cagatggcac gatttatatc aaacgagtaa tcggtcttcc aggagaaaat gtcagctacc
     2281 aaaatgatca attgaagatc aatgggaaag tagttaagga accttatcta actaaaaacc
     2341 tgaaatcaga tcatgcaaat gcatcctata cgacggactt tacactacaa gagctaacgg
     2401 gacaatccaa gcttccggaa gataattatt ttgtacttgg agataatcga agagtttcaa
     2461 aagatagtcg ttcgtttggc acgatcaata aaactgatat tctaggtaaa gcaagatttg
     2521 tctattatcc acttgatgaa ataaaatgga tccagtgaga aacaataaag cgtgcagtct
     2581 gttgagactg cacgctttat tgtttaatcc aaaatcagtt tactacctta gtacgtaaaa
     2641 tcaaacagct cgtttaaaca cttttctagc ctctaaagtt ttgataaatg aataaacaac
     2701 cattatttga atagggattt ggatcatatt cctgatgaat ctacctagaa aaattacttt
     2761 gaaatctgca ccaaacaaaa cagacatcag ccaagaattg ataaaaaggc tgactgggat
     2821 cataatcaat aattgggcag cgatgacgga aggcaggttt atctttttat gatataaaaa
     2881 taagccatta ataaatccac caaggaaagc cgtgataaga aaagcagctt gaaacgttcc
     2941 tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt tcaagcaaac cagcccagat
     3001 cggtccatat agataaccca taagcgccat acttaaaaat ccgaatgtga tttgaaaata
     3061 agcaggacca atcgctatct tgtataagat gacatcaagg gctaataaaa gagcagaggt
     3121 cgacaacaat cttgcattca tttcgggcaa tctaaaaacg tcttttgcaa tcaaagtgtt
     3181 caaaatattc tctcctttgt cagttaataa taaaaggata ccataaaatc accaatgtgt
     3241 catgacacat tggtgattta catacactgg catctaacaa ttaaaaaaag ggtgagaaga
     3301 ctgcttttat aaactgtaaa aaagagattg tacgaataaa aaaaatagag gcggagaata
     3361 cttatcgtat catccgcctt tgtttttttg ttataaaaaa cgtcagatta gttatctgct
     3421 tacatcttca tccatacggg ttctaatgta tttccgtcta gatcttggac ttctaatgaa
     3481 aacatttctt cttcaggcat tcccatatct acttcgtgga aagtaccacc attttctttt
     3541 gcaatttgag caaatttttt tacttcatcc gcattttcaa cactaaacga aatcaaagca
     3601 ccacttgttt ttttattatc agctatttct ttatctttta taaatttttt ataaaaatca
     3661 tgagagagaa gcattatcca aaaagtgtca tcccatacca ttgaaccagc ttcttcagta
     3721 gaaaattctt catttttttt aaaacccagc ttttcataaa aagcaacgga acgattgaca
     3781 tcagcaacag gaaaattaac aaaaaccata ctattcattt gtttcgtctc ctttctgttt
     3841 tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa atttagattc atatatagaa
     3901 accaaaataa aaatttgcat catcaaaaaa gtgaagatgt gatagaatga ggactggttc
     3961 actttcactt aactaataga tggaatttct ttat
//
LOCUS       DACIRY010000179         3992 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.186, whole genome shotgun sequence.
ACCESSION   DACIRY010000179 DACIRY010000000
VERSION     DACIRY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.186"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(51..1517)
                     /locus_tag="IYE22_002620"
     CDS             complement(51..1517)
                     /locus_tag="IYE22_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ9507800.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IYE22_002621"
     CDS             1904..2500
                     /locus_tag="IYE22_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ9507801.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IYE22_002622"
     CDS             complement(2558..3040)
                     /locus_tag="IYE22_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507802.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IYE22_002623"
     CDS             complement(3146..3343)
                     /locus_tag="IYE22_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507803.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..>3992
                     /locus_tag="IYE22_002624"
     CDS             3638..>3992
                     /locus_tag="IYE22_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ9507804.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEG"
BASE COUNT         1374 a          786 c          650 g         1182 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg at
//
LOCUS       DACIRY010000180         3886 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.187, whole genome shotgun sequence.
ACCESSION   DACIRY010000180 DACIRY010000000
VERSION     DACIRY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.187"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            51..737
                     /locus_tag="IYE22_002625"
     CDS             51..737
                     /locus_tag="IYE22_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ9507805.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            739..1683
                     /locus_tag="IYE22_002626"
     CDS             739..1683
                     /locus_tag="IYE22_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ9507806.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1882..2526
                     /gene="pcp"
                     /locus_tag="IYE22_002627"
     CDS             1882..2526
                     /gene="pcp"
                     /locus_tag="IYE22_002627"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ9507807.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2605..3882
                     /locus_tag="IYE22_002628"
     CDS             2605..3882
                     /locus_tag="IYE22_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507808.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1241 a          675 c          860 g         1110 t
ORIGIN      
        1 tgttttgctt tataattaaa aaaatattgt cattttatgg aggaaaatat atggaaaatt
       61 atcttagcct tttaggcgtc ttgattgtta ttctcgggtt tgcatttaaa ttagactcga
      121 tattgatcgt aatggttgct ttagttgtta cagctttgac tggcggtctt ggcattcaag
      181 gaatgttaga acttttaggt acaagttttg taaacaatcg cgggatggcg atttttatca
      241 tcattatgtt ggcaacagga acgcttgaaa gaaatggtct gaaagaatca gcggcttcct
      301 tgatcaagcg attcaagaaa gtatctgctg gtatgattgt agatatttat gggatctttc
      361 gaatgatctt tgcggctttt aacgtgagtt ttggtggagt tgctggattt gttcgtccaa
      421 tcattttacc gatggcattg gggaccatcg aatcaaaagg gttagaagtc catcctaagc
      481 atgaagaaga actaaaaggt atggcttcag ctatggaaaa tgtctgctgg ttctttggcc
      541 aagtactatt catcggagga gcaggggcgt tgctagtcca atcaacactg aaagatctag
      601 gatatgacgt aacattggga cagcttgctt tagtggaagt tccagtggct atcacggctt
      661 tagttgtggc aagtgtctac ttctatctta aagaaaagaa attagcgaag aaatattatc
      721 caggaaaggg gcaataagat ggactttttt acaaatcctg atattttatt aggcgataaa
      781 ttactggaga tcctttatat cgtgatggga ctcgtattga tttacactgg agtaagaaat
      841 ttaacagata aaaccaatcc tcatcgttat gggtcagcct ttttctggac ggtcttaggg
      901 gtagttattg caggaggaag atggcttccg gcaatcgtga acggtggctt gatctttgcg
      961 atgaccatac cagcaattgc taagaaagtc agcaaaggag aaagccggct tccatcaaaa
     1021 gaatatatgg aaaaaatgtc agacaaacta ggaatgaaaa tttttattcc agctttgagt
     1081 atcggtgtct ttgcgatctt gtttgctcta tttaccaatt taggtgcttt agtcggcgtg
     1141 ggcgtcggtg tttttgcagc catgctaatc ttgatgttct tttctcgaga taatcgacca
     1201 agtacatttt tagatgatgc agcagatatg ctgggcacag tagggccgtt aagcatgttg
     1261 ccaatgcttt tagcttctct aggtgcggta tttacaagcg ctggagttgg gacagtgatt
     1321 tccaatgcag taggtacgat cgttccagaa ggaaatgtga acatcggcat catcatttac
     1381 gctcttggca tggtgatatt tacggtaatc atggggaatg catttgcggc catcaccgtg
     1441 atgacggttg ggatcggtgc accattcgta tttagtcatg gagcgaatcc agcgttaata
     1501 ggaatggttg cattgacttg cggtttttgt gggaccctgc tgacaccgat ggctgctaac
     1561 ttcaatatcg tgcctgtagc gatgctggag atgaaagaca aatacggagt gatcaaaaac
     1621 cagttgttta ttgcgttatt tatgctagtt ttccaaattg cttatatgat tctatttaaa
     1681 taatagaaga gaggggttgt gacagaataa ttgtcgcaac ctcttctaac attgtagtgg
     1741 aacactattt ttttataaga atatattaag tttgatctgt tatgttattt aaactattgt
     1801 ttcctttatt tacgctgttc aaggaaaatg gtatgataaa agataaccat atgttaaatc
     1861 aatcggacgg aggaacaaca gatgaaagta ctagtaacag gttttgatcc atttggtggg
     1921 gacaaagtga atccagccta tgaagcagta aaaaaaatgc cagatgaaat cagcggtgct
     1981 gaaatcatca aagtggaagt accgaccgta ttcgaaaaaa gcagtcaagt agtcaaagaa
     2041 gccatccaac agcaccaacc agatgtcgtg atctgtgtcg gacaagcagg tgggcggtca
     2101 gcagtctcgt tcgaacgtgt cgcaatcaat ctagcagaag caagaatccc tgataatgaa
     2161 ggaaatcagc cattcgatac ggctttagaa gaaaacggtc cagccgctta ctttacctca
     2221 ttgccaatca aagcaatgac aaaaaatgta caagatcatg gtctaccagc gtacatctct
     2281 tatacagcag gaacatttgt ctgcaatgat atcatgtatc gtttgcttca tttgatcgaa
     2341 acacaattcc cggcaattcg aggtgggttc atccacgtgc cattctcacc cgatcaagtg
     2401 atcgatcgtc cagtgggtac gccttctatg tctttagaag acattgcttc ttctttgact
     2461 tatgcagttg aagcagcagt agaaaataaa gaagatattt ccggaaatgc cggaacaact
     2521 cactaattta gcaagaaaca ctggctggtt gctaacgtta tgttagtagc cagctttttg
     2581 taattagaaa ggaaagaaac agccgtggaa gaatggcaat ccgtctttga agaatggttt
     2641 ccaaaagaaa tcagcaaaag ctatcccatc aaaatttcta aacaatacac aagcagtcaa
     2701 cgttgggaga tctatgcaaa attaacgaaa aaacagcggg aactagtcga taagcaccgc
     2761 cgatatctaa tcagctctcg ttttatggaa gaacattatt tagctgcaac agattgggtg
     2821 ttttctgact tcaagatcaa tccgtttttt cgaacaaaac gaagccagca aaaattgtat
     2881 tgtgagtgtg gtagagaatt aaaagtgcaa tacatcgtta aatcgccaaa aaccggaaag
     2941 atcctaaaac taggcatcaa ccactttgcc gatcacctgc atgtctctcc gacagtggca
     3001 gcttcgatcc atcagggaat gacaaaagtt gatctggcct tagacgaatt attatggctt
     3061 aagcagaaga atatcgattt tcctgaagga ctatggcaaa aatactgttt tgtcttgtac
     3121 caaaatcgtc ggatgaaaca gccatattta ccggatataa aattagctca gcggctcgca
     3181 gaatttcgcc aagtagaaat gccgatttac atagcggatt atcaagcatt ggaaaatgaa
     3241 atcaagaaaa taagtgaaca catcaatggg cagtcaaaaa agcgacagat aaaaaaagaa
     3301 ctatttgacg actttgcaga agaattagta aaggatgtag aagaattctt gatcaactat
     3361 cgcgcatttt tacgtaaaga ttggcaatcc atcgtatatg aagaagtgcc agtacatcct
     3421 aatgcttatt ttgaaacatt tatttccgtg ttgagaaaga caaaacgtca acgaacacca
     3481 gaagtcactg ctcagatgga atattttgcg aaaaaccaac gattcatcca accaaaaatc
     3541 tatctattta tttggaagca atattgtcgc tatggtttta cagaagggtt cttcgacagc
     3601 attcctcgga tagtcagaaa tggtttttta aaagtattgc gtaaagaacg agaagctata
     3661 caatctgctg ataagaaaga ccgtacagtc tcaaaagaaa aatggcaact agtagtgaaa
     3721 gatatccaat ctggaaatgt gcaagagaca atcgacaaat ggaaaggaaa acattatcgt
     3781 tttactgaag ctcagaaaca agcgttggaa tattatcaga agttagaaga atcactgcgt
     3841 ttcaatgatg aggcaagaaa gtatctgaag gaattactat aaaaaa
//
LOCUS       DACIRY010000181         3834 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.188, whole genome shotgun sequence.
ACCESSION   DACIRY010000181 DACIRY010000000
VERSION     DACIRY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.188"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            352..1041
                     /locus_tag="IYE22_002629"
     CDS             352..1041
                     /locus_tag="IYE22_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9507809.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IYE22_002630"
     CDS             1028..2236
                     /locus_tag="IYE22_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9507810.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IYE22_002631"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IYE22_002631"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ9507811.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1261 a          662 c          775 g         1136 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agta
//
LOCUS       DACIRY010000182         3718 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.189, whole genome shotgun sequence.
ACCESSION   DACIRY010000182 DACIRY010000000
VERSION     DACIRY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.189"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            363..731
                     /locus_tag="IYE22_002632"
     CDS             363..731
                     /locus_tag="IYE22_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ9507812.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IYE22_002633"
     CDS             911..1852
                     /locus_tag="IYE22_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ9507813.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IYE22_002634"
     CDS             1921..2652
                     /locus_tag="IYE22_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ9507814.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..>3718
                     /locus_tag="IYE22_002635"
     CDS             2796..>3718
                     /locus_tag="IYE22_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ9507815.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFG"
BASE COUNT         1244 a          623 c          824 g         1027 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcga
//
LOCUS       DACIRY010000183         3686 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.190, whole genome shotgun sequence.
ACCESSION   DACIRY010000183 DACIRY010000000
VERSION     DACIRY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.190"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(65..934)
                     /locus_tag="IYE22_002636"
     CDS             complement(65..934)
                     /locus_tag="IYE22_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ9507816.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(1226..2836)
                     /locus_tag="IYE22_002637"
     CDS             complement(1226..2836)
                     /locus_tag="IYE22_002637"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ9507817.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(3058..3303)
                     /locus_tag="IYE22_002638"
     CDS             complement(3058..3303)
                     /locus_tag="IYE22_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507818.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFGTHKGLKK"
BASE COUNT         1093 a          773 c          602 g         1218 t
ORIGIN      
        1 agaatggaga gcgacttcaa aagtcgttct ccattcctat atatcgtatc tgttatgtgg
       61 atgcttacgc tttgttagct gaaccgaacc agtcgatatg agatttagca tcttcaacga
      121 ttgctgcttt acctggagct aataatttac gagggtcgaa tcctttacct tctttatctt
      181 taccagcttc gatatattta cgagtagctg cagcaaatga taattggaat tcagtgttaa
      241 catttacttt tgagataccc attgagatag ctttttcgat ttgatcttga ggaatacctg
      301 atccgccatg taatactaaa ggcatgtctg gacctacagc atcagcaatt gcttgtaagt
      361 ggtcaaaagc taatcctttc cagttttctg gatattgacc atggatgtta ccgataccgc
      421 aagctaagta gtcgatacct gtagcaacca tttgtttgca ttcttcaata tcagctaatt
      481 cgccgttacc gatgattccg tcttcttctc cgccgattga accaacttca cattcaactg
      541 agataccttt tgcatgtgct ttttcaacaa cgtctttagc taatttcaag ttttcttcaa
      601 atggtagatg tgaaccatcg aacattactg aagtatagcc taattcgata cattccaaag
      661 cagcttcgta atcaccatgg tctaagtgga tagcaactgg aacagtgatg ttcattgctt
      721 caactaaatc agtgattaaa tctttacaga ttttgtaacc acccatgtat ttagctgcac
      781 ccattgatgt ttggataagt actggtgctt gttttgcttc agccgcttca aggattgctt
      841 gagtccattc caagttgttt gtgttgaatc cgccgacagc gtaaccgcct ttacgagcag
      901 cttttaaaaa ttcagctcct gatactactg gcataataaa attcctccta agttttgtaa
      961 aactaaattt gttaagacaa ttcttaacca acgaccctat tttaccaaac atttcggtag
     1021 ttttctacta aaatcaatga aaatgaaaat tttcaagaag ttttttgcaa aaaataaaat
     1081 atccaatagc gctttaatcg aaaaaagtct tttgctttct atcttttttc ctacaaataa
     1141 taacaaatca gaaaaagaga aaagtgacaa gaaaattgct tatccccttc tcttgctact
     1201 tttctctttc tatcaagttc aaaaattatt ttgtttcgtg atctgctaat gatgctccga
     1261 caaatgcttt gattaatttt tgcgggcgat ttggacgaga aatcaattct gggtggaatt
     1321 ggcatcccac aaagaatttt ttctctggca gctctacgat ttctactaaa cggttatctg
     1381 gtgagacacc tgaaaagact agcccttgtt cttcaaacaa ttgacgatat ttgttattga
     1441 actcatagcg gtgacggtga cgttcttgca caacatcggc tccatcatat gcttcagccg
     1501 tcttcgttcc ttttttgatt ttacatgggt ataatccaag gcgtaaagtt ccacccaaat
     1561 tctcgatgtt ttcctgatca gccattaaat caataatatt gtttgctgta tctggattcg
     1621 tttcagctga tgacgcatct tctaatccaa cgacgttacg tgcaaattcg acacaagcca
     1681 tctgcatacc aagacaaata cctaaaaacg gaacgtcatt ttcgcgagca taacgaatcg
     1741 cttcgatttt cccttctact ccgcgatcac caaatccacc aggaaccaag attccatctg
     1801 cgtctttcaa acgttcttct acattttctc tcgttaattc ttgtgctttg atccaatcga
     1861 tctcgatgtc tgaattatag gcgaaaccag aatgtttcaa tgcttcaact actgagatat
     1921 atgcatctgg taactcgaca tatttaccga caagcgcaat ccgtactttt ttcttcaagt
     1981 tcaagacacg ttcttctaat tctttccatt ctgtcatgtc tgcttctggt gtatctagtt
     2041 ttaaatggtc gcaaacaatt tgatccattc cttgtgcttg taaaactaat gggatggagt
     2101 aaagtgtatc tacatctggc gactcgataa ctgcttctgg ttcgacatca caaaattgtg
     2161 ccaatttgtt tttcacgctt tgagaaacag gttgctctgt acgtaccacc aaaatattcg
     2221 gttggatacc taaaccacgt agctctttta cactgtgctg cgttggttta gttttcattt
     2281 ctccagcagc tttcaagtaa ggaatcaatg ttgtatggat atacataaca ttatctgtac
     2341 cgacatctgc tttcatttga cgaagtgctt caaggaatgg caaagattct atatccccta
     2401 ctgttccccc tacttctgta atgatcacat ctgaatcagt cattttagct gctcgcatga
     2461 ttttttcttt gatctcattt gtaatatgag gaataacttg aacagttgct cccaagtatt
     2521 cgcccttacg ttctttacgc aaaacttcag aatagatttt tcctgtcgtc acgttggagt
     2581 atttgttcaa attgatgtca ataaaacgtt cgtagtggcc cagatccaaa tctgtttcag
     2641 caccatcatc tgttacgaat acttctccgt gctgatatgg gctcatcgtc cccggatcca
     2701 cgttgatgta tggatcaaat ttttggatag ttacttttaa gccgcggttt ttcaataagc
     2761 gtcctaaaga agctgcaaca atcccttttc cgattgatga aacgacaccg ccggtcacaa
     2821 aaatatattt tgtcataagt tcaaaaactc ctttttaatt tatgtgcagg aaaagtctca
     2881 gacaataaac aaagctccct attcttcaat aagaataggg agccgaaatt tctgtaaact
     2941 tctgaccctc taaaagggtg cccaaggtgt ataatacaag ctgactaagg gaaagtcaag
     3001 tactcaaatg aatactatcc gcttacaatt ttttcttttt cgatatcctt tgagaaatta
     3061 tttcttaagt cctttatgag tgccaaaaaa atttaaccca cataataagc ttgatataaa
     3121 catagctaac gttaaaccta agcaaatcag aaaatcgtta ctgaaaaatt ttaagtgaaa
     3181 taatagataa aaaaataaaa caaacattat gatagataat atcccaccta acaaagaact
     3241 actttttcct acttgcagca acagtttata tagcttatcg tagaaacaat ctttttcttt
     3301 cacccttatt tacctccttt acgtaaaaaa cgtagtccac agttctcgga tttttggcat
     3361 atcttttcta ttaaataata tcaaaaacaa aacaattcca ctaatttttt caaagatgac
     3421 ctttttttca acaaaagtgc cttgtaggga ttcattgaag gtaacgaatc acataagtaa
     3481 aaaataagga gatgcgacaa aagtcgtgcc tccttatttc taaataaacg gtgttctcct
     3541 gccaactcct cggtcacaag tcacaatatc catcaaacat gagaagctgt ttttgaaatt
     3601 atttcttgtg attcggagtt aaatggtagg ctcacaacct ctggataaac ggtattcaaa
     3661 agccagtttc tcggtattaa gccaaa
//
LOCUS       DACIRY010000184         3526 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.191, whole genome shotgun sequence.
ACCESSION   DACIRY010000184 DACIRY010000000
VERSION     DACIRY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.191"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..108)
                     /locus_tag="IYE22_002639"
     CDS             complement(<1..108)
                     /locus_tag="IYE22_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ9507819.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLE"
     gene            complement(213..1076)
                     /locus_tag="IYE22_002640"
     CDS             complement(213..1076)
                     /locus_tag="IYE22_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ9507820.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1272..3371)
                     /locus_tag="IYE22_002641"
     CDS             complement(1272..3371)
                     /locus_tag="IYE22_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ9507821.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT          805 a          706 c          597 g         1418 t
ORIGIN      
        1 ttccaaaagc acaagcagag tagcttctct ttgcggtgtc agcttaaacc ccgcttcatg
       61 tagctgctgt tttgtttttt tcatcgccga aatagcattc atgaccattt actcaacccc
      121 aacaatttat aataattaca aataaaaaaa gatataacac tatgttaatt aaaattatta
      181 taagctagtt aagaaaaaca tgcaagggat tctcaattag attttttgac tagttttgtc
      241 acaccgtttt cttgtgtaat caatccttgt ttcaaaagat tacctaatgc gcgcttaaat
      301 gctcctttgc tgataccaaa agctttcatg atttcttctg gatccgattt atctgtaaag
      361 ttgatttgct gatctgcaga acgttctaaa atggttaaaa tcatttgtgc atcatctgat
      421 atagcttcat atgctcttgg cttcaatgaa agattcagta caccgtctgg tcggacacca
      481 atcactcgac cttttatctg ctcgcctaga cgaggttcat tatatcgttc agaaggatgg
      541 ataaacccaa gatagaaatc ttccgttaaa acgtacgtac ctgttaattt caagcggtat
      601 gctgttccta tcacatcttt gtttttcatt tcttctgttc catatttgct taaagatttg
      661 aatatttttt catctgctaa actgccccaa atacggtctt ttgcatcaac tctaagggaa
      721 atcatcaagc gatccccttt tttaggccat agctctgtca ttgtcggcaa ttcatcaaga
      781 gatacgacaa agtctttatc tggaagaccg atatcgacaa acgcacctaa atcttttcgt
      841 gtagcagtaa cgattccaaa agcgtaatga ccgatacggc ttttaggaat ttgtgtagtg
      901 atccgatttt catgtttttg attttggtaa ccgaatcctt caactgcttc accgattttg
      961 tgttcacctt ctgatttgct taagccaaag gtttgtccgt ttttttggac aaagtagttt
     1021 ttttcatttt catcaataat aatacctgta aatatagttg ctaataaatc gttcattttt
     1081 ttctccctct atagtgaaag gttttcttaa tacatttctt tgctatcata acataatgtt
     1141 atctacttga atagctgagc cttggtttgg aaaggtgaaa aaaggagtat gacaacagtt
     1201 gtgtcatact cccctttttc gaataaacgg tgttaaacgg caggctcaca gcctctttca
     1261 tttgcctaaa attaattgct cccatttcct ccgaaccatc cagttttaca gttgatggtt
     1321 acgatgtaaa atggcaaagc actttctcca atgcctttca tcaatgcaga ttttgttgaa
     1381 gtggcatata gattataata gccttcccca ttttcgattc tggctttttc taatctgtaa
     1441 cctccttcaa cgatatatcc acgttggata catgtagcaa tcactttgct ttggacacct
     1501 ggtgcccacg tccatgctgg attagatgca tcggcaatat cacttgctct ggcagatttg
     1561 attggctgat tcgctgagtt tgaagaggaa ggggtcgagg atgccgcttc ttgctgtgct
     1621 gcttgtgcga ccgcagcttg ttgagcggct tgagctgcag ctgctgcttg agcggcttgt
     1681 tttgcttgtt cttcagctgc ttttttcgct tcttctgctt ttttcttttc ctcttcttct
     1741 tttttctctt tatcagtgag catcttatct acttgtttca attgttcatt caactgagca
     1801 gatagatctg tatttttcac tttttctact gcttcttttg ccgtcttgta ttggtcgcgt
     1861 gttgctgaat ccactacttt tccgtcttta aagactatag ctgttttttc tttagctgtt
     1921 tgcaattcag catattgatt tttagcttct gtttgtgctt gttgaatcaa ttttccaaaa
     1981 tctgtgttat ccgtttcgat tgcttggatc tctttatctg ctttcaactt gatgtcatct
     2041 gctagatgat catcgacgat ggcaggtgct atgaaccact cattcacttt ttggatatct
     2101 cggatcttag attggatatc ttggtaagtt gtttctaact ctgaatatcc tttttcattc
     2161 ttaagactgg ataaatcatt ttttagtttt gataaatctt gatgaatcat atctgcacga
     2221 ataaattgat ggtgatcatc tgtataaaag gcagcaagtt tctcatgaag ttcttccatt
     2281 tgttgctgtc tttttgcttc tgctgctact tcggcttcta ctttttgatg atggtcataa
     2341 taagcccagc ttcctccacc gagtagaagt actgctgcta aaacgagata aggtactttt
     2401 tttgattttt tcttttgcgc tttatctgga tctgtttttt ctaagtttga ttcttgatgt
     2461 gttttttcag tatctggcac gattgttatt gatttcgctt ctgcttcaaa cagcttaggc
     2521 tggttctctt ctttttctat ttttgcttca agatcagtac cctctttgct tacagattga
     2581 ttttcagcat cggattcact atttatttca tcgggctctg ttgacttttt gtctgtgttt
     2641 tcgagtattt cttttttctc tgcttccggc agtttattcg ttttttcctt ttctaattgt
     2701 ttttcagtaa atacttcagt tttttctgtg atatccgttt ttacgtcttt tatttttact
     2761 gtttctttac ctgtggcgtt ttctctatca gagtcttttt cagtttcttg ttccttttct
     2821 ggctctactt tgttttcctg tgcaattttt tcactgatca ttttgagcag ctctttcgcg
     2881 tgctcttctt ttgtatcttc acgatgtgct ttgatataag cggatagtat cgaattttct
     2941 tcctcattat cgacgatttc tttcagttca gctactcttc ttttttgttt tgattcaagc
     3001 gtttcttctt gattctttcc tgattcttcc gaagtctcat gaacagttgc tttcttctgt
     3061 tcatttttct ctatattagt tacttgatcg tgagcaggtg gttcttcgtc ttctggttct
     3121 gctttttcga ataattccat tacagattct agaggtaaat ctttcaattc cgaccattcg
     3181 attggatcgt ttaattctgt atcaggaaag actgcatctt cagttgattt atcctgcata
     3241 gctgcatctg tatttgtttc ttcaattgat tcttttattt gctgatcttc tgcagttatt
     3301 tgatgaccgc agtctggaca gatagtattc tgctccattt ttttcgatcc gcaatttgga
     3361 cattttttca tcacttagcg atcggctcct ttactaggtt tacgctacca tttaatctat
     3421 catattctat acgtcacgcc aatagcttat ttttaggatt tacttaaaaa taatgaatga
     3481 atttccttcc tatagaaaaa aaagcttgag actgcgtagt ctcaag
//
LOCUS       DACIRY010000185         3407 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.192, whole genome shotgun sequence.
ACCESSION   DACIRY010000185 DACIRY010000000
VERSION     DACIRY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.192"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..153)
                     /locus_tag="IYE22_002642"
     CDS             complement(<1..153)
                     /locus_tag="IYE22_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ9507822.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIM"
     gene            581..1567
                     /locus_tag="IYE22_002643"
     CDS             581..1567
                     /locus_tag="IYE22_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAQ9507823.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            complement(1692..2126)
                     /locus_tag="IYE22_002644"
     CDS             complement(1692..2126)
                     /locus_tag="IYE22_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9507824.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            2241..3215
                     /locus_tag="IYE22_002645"
     CDS             2241..3215
                     /locus_tag="IYE22_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9507825.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
BASE COUNT         1114 a          621 c          694 g          978 t
ORIGIN      
        1 catgattttt atagctcttg gattcaagcc atgtgtctcg atccccgcac ttcttatctc
       61 aaacattgaa ggagacaagt atttacgcgc gtagccttct gctatttgac tgcggcatga
      121 attgcccgta cataagaaat agattttttt catttgattt cctccgttat ggattagtaa
      181 atgtcactgt ttatctaaag taaactctat gtgatggagc gaaaaaacaa tgggtttctt
      241 agtttatata tttctttttg ttcagcgtat aaatcgatgt gtacaaagaa ctaatcttgt
      301 cttttttacc ataaatacat gttagaaaca atcattttga gtcaaaaatc aacgatgtaa
      361 cagactgtta aaagagatta agacacggca gatgatgaag aaagtagggg tgtagtaagc
      421 cgatacattt cttgattgtg aggcgagaac aattattcgt cttttaagaa aaaaagtagt
      481 agaatcagtt catattaaaa ataaaaaacc ttccattttt gtaggtttaa aggaggtcat
      541 taccttgaaa aaaatcggct ttttaagctt tggacattgg atggagcaag gatcgcttgt
      601 gaaatcagct caagatgctt atttgcaatc gattgattta gcggtggaag cagaaaaaat
      661 cggagtggac ggagcgtttt tccgcgtaca tcattttgcc cctcagattg ggtcgccgtt
      721 tcctttgcta gcggcaatcg gcgcaaagac aagtaagatt gaaatcggta caggacttat
      781 tgatatgcga tatgaaaacc cttattatat ggcagaaaat gccggattga cagatatcat
      841 cacgcaagga agagtgcaac taggagtggg gcgtggttca cctgaacaag ttctagatgg
      901 ctggaaatac tttggttatg attacagtga agaagaaatc aaagaggtaa cacgggaacg
      961 cacccttgaa ttcttggaat tacttgaagg aaaaccattt gaagaaccaa atcctcaacc
     1021 aatgttccca caaggatcag gaaaattacg gatagaacct tattcagaag gattacgaca
     1081 aagaatctgg tggggagctg gttcgcagca aacggcaatc tgggcagcac aacatggaat
     1141 gaatctgcaa tcatctacat tagtcaatta cgaatcccac gagccattcc atatccaaca
     1201 agcaaaccaa ttacgtgcgt ataaagaagc gtggaagcaa gcaggtcatg attttgaacc
     1261 aagaacatta gttactcgct cgattcagcc aatcaccact gatttggatc gacgcttatt
     1321 tggacaagac ggtagtccgc aacaagatgc tgtcggctat cttgcttatc accgaaaccc
     1381 aacgattttt ggaaaaactt ttgctggtga acctgatcaa ttaatcaagg agctagctga
     1441 agatgaagca atcaaagaag cagacacagt acttgtcacg attcctaata ctttgggggt
     1501 agaatataac atgcacttgc ttgaaacaat cgtaaaagat atcgctccag aattaggatg
     1561 gcggtaaaaa ggatattttt gtaagtgcag gatacttgag tgaaaaaata aacacttcta
     1621 acaatcagtt agaggtgttt attcatgttt tcatgaagtt catagagaag gagagtgagt
     1681 tatccgtttg ttcaatgaga atgtacataa gcttctttta catgttgggg taaatcatca
     1741 ggaatcattg tcaatacggc ttcttcattg ccggctttta ttgcttcttc atagtaccag
     1801 cttttgtagt ttagcatagc gagtactctt tctaattttg ctatttcttt ttctactact
     1861 tctttttgtt tttcaaatag ttctttacga acctcaaatg tcttactgcc ttctttgctc
     1921 cattccatga attgcttaat gtcttttatt tctaaaccag attttttcaa acagtcaatg
     1981 actcttaaag cctctaactc attctctgaa aattttctaa taccagaaac acgctggata
     2041 tcaggaaata atccctcttt gtcgtaataa cgtaaagtag aaacaggcaa tccaaacatt
     2101 tcagacactt gtccaatgga atacataaaa aaatcctcct aaaataaaat ttttaccttg
     2161 acctaaagtc aagtttaagt tttatcataa taatacattc aaaaacaaat gtcaattttg
     2221 atcaaaggag agtctagaat atgaataaag aattaaacat cacacaagaa tgggacaaag
     2281 tatttccaaa aagcaatcaa gtcaatcatc gaaaggtgag cttcccgaat cgttttggta
     2341 tcacattagt cgctgattta tatgaaccaa aagaagtaag tgaaaagaaa ttgccggcaa
     2401 tcgctgtgag cgggccattt ggtgcagtga aggaacaatc gtctggtcta tatgcgcaga
     2461 cgatggctga aagaggcttt ttaacgattg cgtttgatcc atcgtttaca ggagaaagcg
     2521 gaggagtacc tcgatatgtt gcttcgcctg atatcaatac ggaagatttc caagcggcag
     2581 tagacttttt atctgtacaa aaaaatgtaa accctgagag aatcggaatc attggtattt
     2641 gtggatgggg aggtatggca ttgaatgcag cagctatcga tacgcgggtc aaagcaacag
     2701 ttgtttccac aatgtatgac atgactcgag taacagcaaa tggctacttc gatgttatgg
     2761 atgaaaatgc ccgatatgaa atgcgtgaag aattaaatgc acaacgcatc attgatttca
     2821 aacaagggga ctacgcacgt gctggaggtg tggttgatcc gttgcctgaa gatgctcctc
     2881 aatttgtcaa agactatcat gcgtattata aaacagaccg cggatatcat gtccgttctt
     2941 taaactccaa tgatggttgg aataggacat cagggttgtc acttatgaat gcaaaactat
     3001 tgcattatat tccagaaatc cgtcatgcag tcttagtcat tcacggggaa aaagctcatt
     3061 cacgttactt tggggaggac gctttcaaag aactaaaagg tgacaacaag gaactcatga
     3121 tcattccagg agcatcccat acagatctat acgaccagac agacatcatt ccatttgaaa
     3181 aaatggcaac attctttgaa gagaaccttg tgtaaccaaa aggatttaat ctaaaagaga
     3241 ttatgaaaaa actgccctgc atcaagtaat aaggacagcc aaaaaagctc ctcatttttg
     3301 acttggttgg actcattagc gtagatgcaa gcttttgaac accgtttata ccaagaggtc
     3361 gtgagcctgc cgtttaactc tgagtcacaa ggagcaattt caaaaaa
//
LOCUS       DACIRY010000186         3381 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.193, whole genome shotgun sequence.
ACCESSION   DACIRY010000186 DACIRY010000000
VERSION     DACIRY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.193"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            254..580
                     /locus_tag="IYE22_002646"
     CDS             254..580
                     /locus_tag="IYE22_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507826.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="IYE22_002647"
     CDS             complement(697..1041)
                     /locus_tag="IYE22_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507827.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="IYE22_002648"
     CDS             complement(1117..2364)
                     /locus_tag="IYE22_002648"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ9507828.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IYE22_002649"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IYE22_002649"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ9507829.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          988 a          666 c          671 g         1056 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agtagtttat t
//
LOCUS       DACIRY010000187         3349 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.194, whole genome shotgun sequence.
ACCESSION   DACIRY010000187 DACIRY010000000
VERSION     DACIRY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.194"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(58..354)
                     /locus_tag="IYE22_002650"
     CDS             complement(58..354)
                     /locus_tag="IYE22_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ9507830.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            709..1065
                     /locus_tag="IYE22_002651"
     CDS             709..1065
                     /locus_tag="IYE22_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ9507831.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1075..1974
                     /locus_tag="IYE22_002652"
     CDS             1075..1974
                     /locus_tag="IYE22_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ9507832.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2491..3222
                     /locus_tag="IYE22_002653"
     CDS             2491..3222
                     /locus_tag="IYE22_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ9507833.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1119 a          552 c          665 g         1013 t
ORIGIN      
        1 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
       61 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
      121 gggaagctta ctccaccgtt ttaataattg atagaaataa ctctcttctg ctttgatttt
      181 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
      241 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
      301 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
      361 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
      421 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
      481 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
      541 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
      601 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
      661 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
      721 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
      781 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
      841 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
      901 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
      961 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
     1021 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
     1081 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
     1141 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
     1201 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
     1261 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
     1321 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
     1381 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
     1441 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
     1501 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
     1561 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
     1621 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
     1681 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
     1741 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
     1801 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
     1861 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
     1921 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
     1981 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
     2041 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
     2101 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
     2161 atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat tgattgaagt
     2221 ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc
     2281 tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa
     2341 atcgggctga taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc
     2401 ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata
     2461 tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca
     2521 gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac
     2581 ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact
     2641 cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca
     2701 agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa atttgggact
     2761 caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg
     2821 tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa
     2881 ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca
     2941 cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa
     3001 acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct
     3061 aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat
     3121 caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc
     3181 actaaattag tttggacatt agaagataca cctaacaact agtttactga aaagaactgt
     3241 taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc
     3301 taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgat
//
LOCUS       DACIRY010000188         3325 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.195, whole genome shotgun sequence.
ACCESSION   DACIRY010000188 DACIRY010000000
VERSION     DACIRY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.195"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            211..477
                     /locus_tag="IYE22_002654"
     CDS             211..477
                     /locus_tag="IYE22_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ9507834.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            504..803
                     /locus_tag="IYE22_002655"
     CDS             504..803
                     /locus_tag="IYE22_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ9507835.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            977..1528
                     /locus_tag="IYE22_002656"
     CDS             977..1528
                     /locus_tag="IYE22_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ9507836.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1674..1967
                     /locus_tag="IYE22_002657"
     CDS             1674..1967
                     /locus_tag="IYE22_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507837.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1960..2256
                     /locus_tag="IYE22_002658"
     CDS             1960..2256
                     /locus_tag="IYE22_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9507838.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2331..>3325
                     /locus_tag="IYE22_002659"
     CDS             2331..>3325
                     /locus_tag="IYE22_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ9507839.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMI"
BASE COUNT         1247 a          457 c          662 g          959 t
ORIGIN      
        1 gatttagaaa aacttactaa acaaagagat atgaaaaact ttaataattg tgtagttttg
       61 ataattataa ctgaattaac tttatttaca aatatatatt atatccatga agcccaatct
      121 tctttaatag aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat
      181 aaaggataac gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct
      241 ttatgttgga atctaatcga tcaaaacgtt caaaagcaat ttcctttaga ctatcttcaa
      301 atttttgaat tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaaccg
      361 gaagaaagaa aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa
      421 atatgggcca ttgattcggg tgagtatcaa acgatgttat taccagaaga atactagtat
      481 ctaaaagaaa ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg
      541 cacagctggt cactgcttct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg
      601 aaattgaaat gggtatgctt cctgaagatg ttcatctacc acgtttagtg atgacacatc
      661 tttatattta ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata
      721 ttaccagcca gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc
      781 agattggagg aacggatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa
      841 gatatcctta ccgcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt
      901 ttcaactagt attgacactt ataacggcaa tggtaattga agtgcttgaa caaaaaatag
      961 cataggagaa aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg
     1021 agatcctcat catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga
     1081 agcggaagat aaattagcag tagtcattac tattcgtcca acagatacag aaccatttac
     1141 gaatgatttg agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt
     1201 tattgctgtg aatctttttt cgtacacaaa agcgaaaagt cctcgggtat ttcttcgagg
     1261 aaatgatgaa caatctttgg aaatattaac gactgttttt acagaaaaga aaatcaatac
     1321 cattattttt gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcaaa
     1381 gactatttac gatcaattga gtgcgaaaca aaagaagatg accaaagtat tgataaatgc
     1441 agaaggtaag ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga
     1501 tagtcaatta ttatttcaag aaaaataaca ttttattttc tcatgttact tataagtagt
     1561 tttacatgct ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat
     1621 gtacccagta atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga
     1681 ttgatgaaac ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg
     1741 aactggattc agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt
     1801 ttccaatgaa aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac
     1861 aggaaaaaac tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga
     1921 ttcaaaaagt aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta
     1981 ggtggtttaa tttacggtgg atattggtta tataaatata tgcttgcgca tccgaatata
     2041 gataaaggaa aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact
     2101 aagaaaaata atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga
     2161 agggaaattt ctactagttt taatccatct gagagtcctc aaggaagaga ccgctgccat
     2221 acgggctatc gacattattg ggaaagaatc gattaaaaga ttaccattta gatcaattaa
     2281 ttgtctgata gaataagtac gtgagggaga aattgaaagg gggaaatata atgaaaaaat
     2341 ggctgttatt actaatagga atgggaattt tattcactgg ttgtacatct gagaatacga
     2401 ggactaaagc gactatagaa actgatacaa caattgagac taaaaaaagt aaaacgcaat
     2461 caactgaaca aactacaaca gaaatgaagc aatctgtgga aacaagtgca agttcaatca
     2521 ctgaatcttc ttctgaagag gttaagaata ctttgtggga tacgaataaa gcttccaaat
     2581 tagaaacttt tgtcacacaa tggggaaaga cattaggtca agaatataaa agctacaatc
     2641 ttcagaataa tgtaagtctg tatggcactc ctttaccaca agccgtgatt aatggcgatt
     2701 ggaaaatggc aatcaatgaa gcaccagtta ccgtacaatg gtcagaagat ggaactggtc
     2761 atgcagattt caatttagta gcagtttatt ctgatgtaga aactggagaa tatttagggc
     2821 agcatgttta ctttttcggt tttcaaaatg gacaacctaa agtttattta acgcaacaaa
     2881 atcaaggtaa tgaaaataat tatctgtact ttaacgaaac tcaaaatcaa cagttaaaac
     2941 aaggtttcag tgatattgtg aatggacaaa ctcctcaaac accagtagta gaagaatcta
     3001 ctcaacaagc aacaactaca acgacacaat ctacaagtag ttcaggaatt tcaagttggg
     3061 aagctgctaa agaatctgtg ctaaataata aagctttatg gttgtatgat tctcctgata
     3121 aaaatgtatc aaatactaat ttaattagca caaatgaata tatgaaagat ctaatgcaag
     3181 atgaaaatgg aagctactat tcaattaata ttgtaacgga tgatgatgga aacacgacca
     3241 tggctggtgg aaaacaattt cgtgtttata cagatggaag aatttctcaa cgtattggaa
     3301 tgactaacaa ttttcaaatg attaa
//
LOCUS       DACIRY010000189         3282 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.196, whole genome shotgun sequence.
ACCESSION   DACIRY010000189 DACIRY010000000
VERSION     DACIRY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.196"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            647..964
                     /locus_tag="IYE22_002660"
     CDS             647..964
                     /locus_tag="IYE22_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ9507840.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IYE22_002661"
     CDS             complement(1055..1420)
                     /locus_tag="IYE22_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507841.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IYE22_002662"
     CDS             1628..2110
                     /locus_tag="IYE22_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507842.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IYE22_002663"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IYE22_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ9507843.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1137 a          499 c          598 g         1048 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tg
//
LOCUS       DACIRY010000190         3220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.198, whole genome shotgun sequence.
ACCESSION   DACIRY010000190 DACIRY010000000
VERSION     DACIRY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.198"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            50..1438
                     /locus_tag="IYE22_002664"
     CDS             50..1438
                     /locus_tag="IYE22_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ9507844.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3220
                     /locus_tag="IYE22_002665"
     CDS             1776..>3220
                     /locus_tag="IYE22_002665"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ9507845.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaaga
//
LOCUS       DACIRY010000191         3203 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.199, whole genome shotgun sequence.
ACCESSION   DACIRY010000191 DACIRY010000000
VERSION     DACIRY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.199"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            670..765
                     /locus_tag="IYE22_002666"
     CDS             670..765
                     /locus_tag="IYE22_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ9507846.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1067..1312)
                     /locus_tag="IYE22_002667"
     CDS             complement(1067..1312)
                     /locus_tag="IYE22_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507847.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1657..1941
                     /locus_tag="IYE22_002668"
     CDS             1657..1941
                     /locus_tag="IYE22_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507848.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1944..2360
                     /locus_tag="IYE22_002669"
     CDS             1944..2360
                     /locus_tag="IYE22_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507849.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2497..3174)
                     /locus_tag="IYE22_002670"
     CDS             complement(2497..3174)
                     /locus_tag="IYE22_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ9507850.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         1065 a          599 c          500 g         1039 t
ORIGIN      
        1 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
       61 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
      121 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
      181 aaaagttgtt agtacccttc tgtatccgtt gggagagtaa ggctcaactg ccccctaaaa
      241 agggttctaa aaaatcttta aaaaataaat aaaataaacg cgcgcgcgcg agcaaaattt
      301 cccgccaaaa aatcacccac tttttaaaat aaacaggttc gcgcatgata ttaaactgcc
      361 aatctagctc cctatggtcg ctgtaagaag gagtgatcgc tacgctctca cattttactt
      421 gcagcagtcg cctttaggct ccttcgccaa aaataattca ttagggactc ctttgtggcg
      481 tcctgagagc tcgtctaagc cgttctaaga caagattcga taaaaactat gtccttcgtt
      541 ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cccaaggcaa taaaaaactt
      601 aggcttattt ttgctttttt attgtataat tgattgtcgc ctatccgaca gtcttgcgac
      661 tggaaggagg tgtgtttcgt gttcactaac tttatttgct cccttcttgt tgggctttta
      721 agcgctttgt tcagttactg gttgaataac cgaaacaaga agtaaaactt aattaaggcg
      781 actcagccca caaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtgtttcg
      841 tgttcactta tttgcaatca aattataaca gaaataaata tttatttcaa aaatatccta
      901 caaaagtcta aaatccgtaa ttatttctta tatgcaacat ttacaaataa agtcgctctg
      961 agagctcgta tttttcgatt taagacgttt tatttagttt ttggtgtatt tcattggtcc
     1021 aagctaaaaa actcttagaa gcgattttag agccatttaa acccacttaa ttatattctc
     1081 catgaaattc gctccatgcg tcaaaagctg cgtctgaaat tccgccgtct tcgttcacta
     1141 gttgcattaa tttttccaac gatcgttttc tttcgggtgt catattttta tattccaaca
     1201 atttttcgtc tgttaaatcg aacttgattg cttgaattgc ttgctctaat ttttctaagt
     1261 tttgaaaata ttcgtaagga aatttttcca ttttttcttt ctccagtctc acaatttttc
     1321 tagaaataaa atcacttctt tgccgtctat actctcaaaa aaaatgtcgt ttgaaaaact
     1381 aaataaattt ttgatcgtgc atttttaaat ttggttatcg attacacgtg cagcactccc
     1441 ccccgaattt taaagagcat aaggaaacgt gtacccactt tctaaaaaaa gtgggtagat
     1501 aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat cactttttcg ccatcgtggg
     1561 tagcttatgt atagcatttt ggcaaaaaac agctatacaa atatagtatt tgtatggtat
     1621 acataaatta tacataattg caaaaggaga ttttttatgg acattttagt tcggaatatt
     1681 gatccaaagt ttgttgcaac aatagacaaa cgttgccaag aactcacgaa taaaacagga
     1741 gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa
     1801 cttacgactt acaaaaaaga ccaattcgat ttattgctcg ataaatttat tgctatccaa
     1861 acagaaaata ctcaaatatt aagcgagtat gtctacacaa ataatcaaat cattgaaatg
     1921 cttatcggag gaatgatata accatgagaa aaacttctgt ccaaaaacat gttactttgt
     1981 accctagtca tgttgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact
     2041 actcggaagc cattcgcttt ttatgtatga gttatgaacc cgataccaac acgaccacta
     2101 ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat ttccatcatg actgaaatca
     2161 tcgcagaatt tgctgattta tatctaaaag atacaggcgt tcttattggc acaaattcta
     2221 aagtatatca agaagtgaaa gaaaaaatcg aagctaaact aaatccatca aatcgaaatc
     2281 gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac ttctagaaat ttcgacccaa
     2341 caaattttct aaaacgataa aaaacagata ggagttattc ctatctgttt tttatttaga
     2401 agccccatac aatatgctag aatgtactag caatcttagt cggttgtaag ggacttgtct
     2461 acttgcttaa tgtaaggggg gcgagttctt ttttatttat tcttctaacc agttacgcag
     2521 agaaatcttt ggcaaatctg acgttaggct tgaaaaagta aattctaatt tggcgatttt
     2581 attgcccttt tttgctttta tttttgtgac tttaagatca ttgaagtatt tttttagttc
     2641 tcgtaatgca ggatttaata ctttttgatc aatgttaccc atttgatagt attctgggat
     2701 atccattaag ctcctaaact cttcaatacg aactacataa taaccagtag aacggtactg
     2761 cataagcagt cggaatagag tttttgcata cgtagaacgc aacgacgtga aagcagataa
     2821 ttcaaatcta ctaaattcag ctgttaaacc attaataata tattctaggt ccggattaac
     2881 actgacttca acaaactttt ttgttttatt gatttcaaaa ccagtaaaca aaacaaagta
     2941 gcgaatagaa tcctcatctt cagtacggta agtaagatta agcatatctt tatagagctt
     3001 ttctaaatca ccgacaaagg cttttgtagc agtcatctta taatcactta actcttttaa
     3061 ttcctcgaaa gaaaatctaa cttttttcaa accacgatct ttcatacgag cgcataatgc
     3121 aaaaaataaa tccatctgct tagcattgaa atttttcagc ggtactaaat tcaattcatt
     3181 ttgatatttg actgttaatt ccg
//
LOCUS       DACIRY010000192         3199 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.200, whole genome shotgun sequence.
ACCESSION   DACIRY010000192 DACIRY010000000
VERSION     DACIRY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.200"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(252..620)
                     /locus_tag="IYE22_002671"
     CDS             complement(252..620)
                     /locus_tag="IYE22_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ9507851.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            770..1252
                     /locus_tag="IYE22_002672"
     CDS             770..1252
                     /locus_tag="IYE22_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ9507852.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1289..2299
                     /locus_tag="IYE22_002673"
     CDS             1289..2299
                     /locus_tag="IYE22_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ9507853.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2414..3127)
                     /locus_tag="IYE22_002674"
     CDS             complement(2414..3127)
                     /locus_tag="IYE22_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507854.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1079 a          517 c          621 g          982 t
ORIGIN      
        1 ttttctcgta ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt
       61 tactaagtgg aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc
      121 cttataaaaa tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat
      181 aggattgaac aaaacctgta taagctgatg aatagtttcc tcgccagtaa aaggtttatt
      241 cgtataaagc tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa
      301 atcaattgtt caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta
      361 tccgtatata cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt
      421 tccggtgcga tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg
      481 tcagtaaaca taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt
      541 atcttagcaa aaaggtcttt gatcttttgt tgatgttctt tttctttgat ctgattgatg
      601 atcggctgaa atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca
      661 gaaaaacaat agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata
      721 tttatatgat aagatgtctc aaataataaa atagaaaaga gacataaaaa tgattgactt
      781 ggcaaagaaa ttcttacacg aaaatatata ttttgagttg tatcatcatc gagccattta
      841 tacaaatgaa gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa
      901 aagcttattc ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa
      961 acaaacggat ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc
     1021 agaacgcatg gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga
     1081 aaaacagatt ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc
     1141 accaggtaga ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca
     1201 gctacttgag atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa
     1261 aatagtgcat acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc
     1321 aacgattggg gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga
     1381 atcagcttta aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg
     1441 gacgcaagat aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat
     1501 gatgcaagat gaaaatatcc aaatgatctt tccgccttgg ggaggagaat tattgattga
     1561 gattctcgat tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga
     1621 tattagtacg ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac
     1681 caaccttatt gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt
     1741 actgaaaaca agagaaggag aatcgattct acagcaacct tctgaaaagt atcaaaaaga
     1801 gtggccccat gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca
     1861 atcagtcagc ggagatgcag taaggatgaa gggacgatta ttaggtggct gtattgatac
     1921 gatcagtcat ttaatcggta ccccttatgg agatgtccaa tcttttaaaa gaaagtataa
     1981 tgatggaaag ccgatcttgt ggtttctaga aaattgtgag ctatcgtcag ttgatttaag
     2041 aagatcactt gttcagatga aactggcagg atggtttgat ggcagctcgg ggatcatgtt
     2101 cggtagaagt gcagcagagg atttgttaga ttataaaatg aaggatgtat atgaagatct
     2161 gtcaaaggaa ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca
     2221 actgacattg atcaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt
     2281 tttgcaaacg tttatttaaa aaattttttc ctgccttgga gtttatcaaa gaccaacaaa
     2341 tctaaataga aaagaaaatg aaagaagctg acagtatttt gccagcttct ttttgtatga
     2401 tctattagaa attttaatta tccataacta tcacattgat cagctgtttc acatcatttg
     2461 gattacctgt aggaatagct actacagatg cattatcaat aatttcttga gttgtttctc
     2521 taatgaacgc tggataagat gtttgatctg caggcatttg ccaatgtgaa ggaactggat
     2581 aactagtgat tgtcccgtca tggttacttg aatacactaa aactccctgg taactgtatt
     2641 tcccagtgag cataaccaca tctttcggat agactgcact ccctgcatca taaggggcga
     2701 taggtgttcc tgcaggataa tgaataacat gtagttcgaa atcacccgat gtatctctaa
     2761 aagtattacc tctaattgct agccaaacac gtgcgtactc aatttgtaga tcagaatatc
     2821 ctgctaacgg atctattgac tgagaagact gtgaggaagt tgtgtctatt gttgaagaag
     2881 aaatatttgt ctcaactgta ctactagatg tagaagaagt attgggctct gagactttac
     2941 tgcttgttga agatgtattc gtttcagtag aagctgattt ctgagtactt gatttttcag
     3001 ctgaactggt ttgtttgatt gaacttttgt ctgtggtcgc agatggatca ctattttctt
     3061 tactaggaga cgtacatcct acaaggaata aagagaccaa caataaaata aaacctaact
     3121 ttttcatata aaatacctca ttattttttt aataccagta tagcataatc ctgaattttt
     3181 gaatccgtaa aaccttgtc
//
LOCUS       DACIRY010000193         3025 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.202, whole genome shotgun sequence.
ACCESSION   DACIRY010000193 DACIRY010000000
VERSION     DACIRY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.202"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..471
                     /gene="purE"
                     /locus_tag="IYE22_002675"
     CDS             <1..471
                     /gene="purE"
                     /locus_tag="IYE22_002675"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ9507855.1"
                     /translation="VIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPDLMFEYA
                     QNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSIVQMPGG
                     VPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQLG"
     gene            455..1588
                     /gene="purK"
                     /locus_tag="IYE22_002676"
     CDS             455..1588
                     /gene="purK"
                     /locus_tag="IYE22_002676"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ9507856.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1601..2896
                     /gene="purB"
                     /locus_tag="IYE22_002677"
     CDS             1601..2896
                     /gene="purB"
                     /locus_tag="IYE22_002677"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ9507857.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
BASE COUNT          958 a          537 c          723 g          807 t
ORIGIN      
        1 gtgattatgg ggagtacttc agattgggaa acaatgaaag aagcatgtca aatattggat
       61 gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc agatttgatg
      121 tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg
      181 ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta
      241 ccggtccaat cacgaacatt aaatggtttg gattcgcttc tttcgattgt ccaaatgcct
      301 ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa tgctggacta
      361 cttgcgatac agatgctttc catgtatgat ttagaattgg aggctaaatt ggctgaaaga
      421 cgttcaatgt tggctaaaac tgttatagaa agtagtgatc aacttggtta aacctttgtt
      481 gcctggttca acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag
      541 tgcaaaagaa atgggcttta aagtaggtgt gctggatcct gtgagcgatt gtccagccgc
      601 acaagttgca gattggcata ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct
      661 tgccagaaga acagatgtca ttacctatga attcgaaaac gtcagtgtag aggcgttaaa
      721 tgcgatttta cccatgtcat ttattcctca agggacagac ttgcttgcga ttacgcaaga
      781 ccgcctgtta gaaaaatctt ttttggaaac gaataacatc gtgattgctc cttatgcaac
      841 gatcgtcagt ccgacggata tccaagatgc gattgacggg atcggctatc cttgtgtttt
      901 gaaaaccact cgaggcggct atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga
      961 tctagctcct gccatggacc ttttaagaga aggaacctgt gttttagaag catggatccc
     1021 gtttgaaaaa gagatttcga tcatggtggc aggaaatggt caaggagatt tcacgacttt
     1081 tccggtcgtg gaaaacatcc accacaacaa tattcttcat gaaacgattg ctccagccgc
     1141 gattgatcag gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg cagaagcagt
     1201 gagcctttca ggtgttttag gggtagaaat gtttttaaca aaaacaggcg ggctatatgt
     1261 gaatgaatta gctcctcgtc ctcacaattc tggacattat tctattgaag cttgttctat
     1321 gagtcagttc gatgcccata ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg
     1381 tttgataagc aatgcagtaa tggtcaatgt tttgggagaa caactgctgg aaagctatcg
     1441 cttgattgag aataagccag actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa
     1501 aggtcgcaag atgggacata tcactatctt gacagaagac atccagcaaa cgctggaaga
     1561 aatcaaacaa acgaatattt gggattagag gagaaaaaac atgttagaac gttatacacg
     1621 ccctgaaatg gggaatatct ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga
     1681 gattttagca gatgaagctt gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa
     1741 aattcgtgag catgcatcat ttgatatcga tcggatcttg gaaatcgaaa aattaacacg
     1801 ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg
     1861 ggtccattat ggtctaacaa gtacggatgt cgtagatact gcttatggct atctcatcaa
     1921 gcaagcaaat gatattttac gggaagactt gaaacgtttt acggaaatca ttggcgaaaa
     1981 agcaaaagaa cacaaatata cagtgatgat gggtcggaca cacggtgtcc atgctgaacc
     2041 aactactttt ggattgaaac ttgccacatg gtattcagaa atgaaacgga acatcgaacg
     2101 atttgaacat gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt
     2161 cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa caattgggga tccgtccaca
     2221 agaaatttct actcaagttc tccctcggga tctacatgct gaatatttct cagcgatggc
     2281 gcttattgct acaagtattg aacgttttgc aacagagata cgcggattgc aaaaatccga
     2341 aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca
     2401 caaaaggaat ccgatcggtt cagaaaatat ggctggtcta gccagagtgg tgcgcggaca
     2461 catggtcact gcctatgaaa atgtcacatt atggcatgaa agagatattt ctcattcttc
     2521 agcagaacga atcatcttgc ctgataccac gattttattg aattacatgt taaatcgttt
     2581 tggcaatatc gttaaaaatc taacggtctt tcctgaaaac atgaagcgga atatgtctgc
     2641 aacatttggt ttgatttata gccagcgcgt tatgctgaaa ttgatcgata agggaatgac
     2701 aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt
     2761 gcagttccgc ccgctgttag aagaagatcc aaccatcaca tcggtattat ctaaagaaga
     2821 aatagacgat gcctttgatt atcatcatca tttgaaaaac gttgacgtga tattcaaacg
     2881 agtaggtttg gaataaagaa gagtgagttg caaaattcaa ttaaaataga atagcaaaaa
     2941 taaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct
     3001 tatcaataat aggaacctga tttta
//
LOCUS       DACIRY010000194         2999 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.203, whole genome shotgun sequence.
ACCESSION   DACIRY010000194 DACIRY010000000
VERSION     DACIRY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.203"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..586)
                     /locus_tag="IYE22_002678"
     CDS             complement(<1..586)
                     /locus_tag="IYE22_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507858.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKIND"
     gene            complement(1224..1481)
                     /locus_tag="IYE22_002679"
     CDS             complement(1224..1481)
                     /locus_tag="IYE22_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ9507859.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1474..1743)
                     /locus_tag="IYE22_002680"
     CDS             complement(1474..1743)
                     /locus_tag="IYE22_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ9507860.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1874..2053)
                     /locus_tag="IYE22_002681"
     CDS             complement(1874..2053)
                     /locus_tag="IYE22_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507861.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(2434..>2999)
                     /locus_tag="IYE22_002682"
     CDS             complement(2434..>2999)
                     /locus_tag="IYE22_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507862.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          914 a          562 c          396 g         1127 t
ORIGIN      
        1 tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac
       61 attcttctat agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta
      121 tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa
      181 acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat attagagtta
      241 aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg
      301 aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa
      361 aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat
      421 ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc
      481 aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg
      541 ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc
      601 taaaatcagc taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt
      661 ctattttttt attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg
      721 agtggggagt acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat
      781 atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc
      841 taaattctat gagaagttta tttttgttgt ttttgctaat taactaaaac tagtacaaaa
      901 aatttaaaat agattattaa acctattgct tattctattc agtcatatat ataagcgtca
      961 tcctttagta actaacatcc cacactacaa tagtattata acatatctat tcaacttcaa
     1021 attgttgaaa tttaagccat ttatatttac ttttcctcaa ctttagaaat gattttacta
     1081 accttatatc cgtaatacgc gtaacaaaca cattcattaa taacaatcaa cactaagcta
     1141 tattagcttt ttaaatgact agggtaaata aaataaggat caaaaacttt taagtttctg
     1201 accctttcct tacttccgat tggttaatag tgatcttttg cagaataaat aaatatcgtt
     1261 tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga ccattttcca
     1321 gataaatcat gctttaatgg ctcaggtttt cctaatccag caaaggggga acgatcgata
     1381 tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc atgccaataa
     1441 agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa cctcgattaa
     1501 gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa ttttttccat
     1561 gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat ctctttttga
     1621 taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt cagcatcctc
     1681 attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag ctactgcttc
     1741 cattcctttc accccttatt tatttctata aaacaattgt acattatatt gtacaattaa
     1801 gcaaatgatt aatattctaa cttcttataa ttaaaagtgc tatcactttt agtttttagg
     1861 ataaaaagtg acgtcacttt ttgagtgctg gtacctcaaa tcctgctttt tcttgggcaa
     1921 tcaataaatc taacgcaata cacataatcc tggattcact tttgccgtac ttctgcttta
     1981 gttgctgcaa ttctttttgt ctcttttcat ccaacgtaac gggtaaccgt ttatttccta
     2041 ttgcaattgg cattagtact ctctcctttc atcgaaataa ccctatgaaa aatatggctg
     2101 caatcatctt gaatagtaga ctcacaaggg ctaatacaaa gcgccaaaag atcaaacaaa
     2161 ataccagtat tgccaaaaat actacgaagt ttaaatgcac agctgcagca tggcgcataa
     2221 gtatttcgcc aacaaatagg actaacaata gaggaatgat tttattcata tggatctcct
     2281 tctttctctt aggttatcgg gtgttgcccg cttatttcgc accacctaag cggcgggggc
     2341 ggttggagcg attggtgcga aataagaacc attcccttag ttactaggtt ttttgtcctt
     2401 atttcacaca ttctcacttt tcggtataaa tgattattgc ctttttttcc tttcaaatcg
     2461 ttgatatggc attcgtttac ggtcaagttc attttggatt tcttctgtat cgtcgtaagt
     2521 ataggtgtct aaaaaatcag ttaaagtcgt gtaccagtcg tcgcctgggt tatctacttc
     2581 ttctacatat tccctcaaaa atgcttcggg tacagcgagt tcataatcca ttcccattaa
     2641 cacgatagat tccgttccac acgtcaaaaa tctagtaaat acaaggtaat aatcgtctat
     2701 cgtcgcatct ttgctgttta gctcttccat ttcttcttcc attaattcca tgctctcaaa
     2761 aattgaataa tattctgatt ctgagtaata gtgtttcctg caaatcgtgt agctgacaaa
     2821 gttttcgacc agctcactcc tttctaggta aacattcatg atgtattttt ctgtttctag
     2881 tcgtagaagc tccgttcctt tttttaccgt ttcaggacga cgtttataca cgtctaaacg
     2941 attatggctg actgtgacct tcgtttgact cgtttcactt tccaaataca attgttctt
//
LOCUS       DACIRY010000195         2995 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.204, whole genome shotgun sequence.
ACCESSION   DACIRY010000195 DACIRY010000000
VERSION     DACIRY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.204"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..178
                     /locus_tag="IYE22_002683"
     CDS             <1..178
                     /locus_tag="IYE22_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9507863.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
     gene            complement(369..1901)
                     /locus_tag="IYE22_002684"
     CDS             complement(369..1901)
                     /locus_tag="IYE22_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9507864.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            2135..2836
                     /gene="nagB"
                     /locus_tag="IYE22_002685"
     CDS             2135..2836
                     /gene="nagB"
                     /locus_tag="IYE22_002685"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ9507865.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
BASE COUNT          909 a          521 c          608 g          957 t
ORIGIN      
        1 tgtatttatg tttatgtact ttattggagg tgcggctggt tcattgctag ggacgacaat
       61 gtggcagcag tatggctggt taggtgtaac ggttagcggg cttgtttttc aaggatgtgc
      121 gttcttcttt caaacagttg tttttaaagg aaagagaaat ctcgaaaata aaaaatagac
      181 gaaaagaggt tgtgaaaagc tgttctgcat caagtaataa gaatagccaa ataaaaatag
      241 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctaattg gccaccattt
      301 attcagaaga aggagttgtg acaaaacttt tgtcacagct cctttttttc aactattcaa
      361 tcaaacgttt attttggttc aatctcaatg actcgttgca caagatcttg ataaaattct
      421 tgttcatgag agacgatgat tactgttcct ggaaatttct gtatcccgtc ttgtaagctt
      481 tctttcgttt cttgatcgat atggttcgtc ggttcatcga gaatcagtaa attaccttgg
      541 ttcattgtca actgtgcaag tttcacctta gtttgttccc caccacttaa tgtagtgaga
      601 ggttcacttg ctaattgatt aactaaccct gctttagcta gttggcgacg taattctttg
      661 atagtcgctt ttggaaattg actgcttaaa tattgtagtg gtgtttctaa tggattttcc
      721 cagtacagat cttgtgaaaa gtaattgatt tttgtgttat ctgggaagtg gaaatcgcca
      781 cttatttttt caatctcacc gatcagtgtt tttaataaag tagattttcc aatcccgttg
      841 aagcctcgaa tggcaatatt ttcaccaaag cggatggtca aattgatggg tgcaagtagc
      901 ggtcgatcat atccgatgac taaatcagtt gtttccactg ctaatgtcgt gacaataggc
      961 gagaatggaa aatcaaagac agctttactt gctgaagctg gaggcgtcag ccgttcgatt
     1021 ttatctaatt gtttttgtcg gctttttgcc atcgttgatc gggaaccggc tttgtatttt
     1081 cgaatataag cttcggtctt ttcgatttgt ttttgttgag catgatattg gcgcaaatag
     1141 ctttcgcgat tttgttcctt ttgtttcaat gcttgtttta gatggccagt atacttagta
     1201 atctttccaa actcgatgtc tgcgatatgg gtggtgatct gatcaagaaa ttcttggtca
     1261 tgggaaatca cgatatacgc accgtcaaaa tcctgaagaa actgcgtcag ccattggata
     1321 tgctgatcat ctaagtgatt ggttggttca tctaagatca gaacatctgg gttttctaaa
     1381 agtagttttg ctaaaatcac ctttgaacgt tgtccaccac ttagattttt caacagtgaa
     1441 tctaaaccta agacatcgat ccccaacccg ctgctcatct cagcaactaa ggtgtcgatt
     1501 tgataaaaca ctccttggtc taattccgtt tggagtttcc cagctttttc cagcaaggaa
     1561 tcatcgaagg tctcgctata tgtttggtaa agaaaagcaa ttttttcttc cttttcgtat
     1621 tcttttgcgt acgcagtttt tagaaattct tcgattgtta atgtttcatc gacttccaca
     1681 tattgatcga ggtaaccttt gtgaatattt ttttgccaat ggatctgtcc gtcatctggt
     1741 aagatctcac cagtgatgat tttaatcaat gtacttttac cggctccgtt ttgtccaatc
     1801 agcccaagat gttcgccagc atttaattgc agatcgacac cttcatagag gattttgtcg
     1861 ccaaattgct gggtaatgtt gtttaatgtc agtatactca taataactcc tttttcataa
     1921 aaaaactgca gacataaagc gtccacagtg aatcacttgc ttggttttca tgcaacatgg
     1981 attgtttgat tgaaccatct tatgaaacct aacacggatt cctatttatt gtcaagaaaa
     2041 tgaaaaaaat gcaatttggt ctatactaat tattgtaaaa agaaaaaaag aatgctataa
     2101 ttgggctata ccaaaatata aaggagcaat cactatggaa atcattcgtg taaaaaacgc
     2161 tgaagaaggc ggaaaaaaag catttgaaat catcaaagaa ggtatgaaca aaggtgcaaa
     2221 agtattagga ttggcaacag gaagcacacc agttacttta tataaagaaa tgacttcaag
     2281 cgatttggat ttcagtgaaa tgacttctgt aaacttagat gagtacgttg gactaggcgg
     2341 ttcagatgaa caaagctacc gtcatttcat ggatgtccaa ttatttgata aaaaaccatt
     2401 caaagaaacg tttgtaccag atggaaaagc agaagatctg gaagcagcat gcaaaaagta
     2461 tgatcaaatc atcgatgcac atccaatcga tatccaaatc ttaggaatcg ggcaaaatgg
     2521 acatatcggt ttcaatgaac ctggtgcttc atttgacgga acgacttcag tagttgactt
     2581 aacagaatca acaatcaatg caaacaaacg attcttcgat aaagcagaag atgtaccaac
     2641 aaaagctgtt tcaatgggaa tcggttcaat tatgaaaagt aaagaaatcg tcttgatggc
     2701 atttggcgaa agcaaagctg atgcaatcaa agggatgatt gaaggacctg taacgaatca
     2761 tttgccagca agtgtattgc aaaatcacga caatgttgtt gtgatcatcg atgaagaagc
     2821 agcaagtaag ttgtaatttt caatagtagg ctaaagaggt tgtgaaatca gcgttttgac
     2881 ctgagtatta gagaagacaa aggaaaaata gctccccata ttttgccgtt tgttgagtaa
     2941 taccgagggt cagctgattg aacaccgttt attcataaaa aggactatga caaaa
//
LOCUS       DACIRY010000196         2992 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.205, whole genome shotgun sequence.
ACCESSION   DACIRY010000196 DACIRY010000000
VERSION     DACIRY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.205"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(106..1053)
                     /locus_tag="IYE22_002686"
     CDS             complement(106..1053)
                     /locus_tag="IYE22_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ9507866.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IYE22_002687"
     CDS             1299..1763
                     /locus_tag="IYE22_002687"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ9507867.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVFPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IYE22_002688"
     CDS             complement(1825..2868)
                     /locus_tag="IYE22_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ9507868.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          989 a          593 c          571 g          839 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gtttttcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACIRY010000197         2609 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.206, whole genome shotgun sequence.
ACCESSION   DACIRY010000197 DACIRY010000000
VERSION     DACIRY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.206"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            314..634
                     /locus_tag="IYE22_002689"
     CDS             314..634
                     /locus_tag="IYE22_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ9507869.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            795..1700
                     /locus_tag="IYE22_002690"
     CDS             795..1700
                     /locus_tag="IYE22_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9507870.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1697..2470
                     /locus_tag="IYE22_002691"
     CDS             1697..2470
                     /locus_tag="IYE22_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9507871.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
BASE COUNT          811 a          463 c          497 g          838 t
ORIGIN      
        1 aatttattgc ctgctaatat ataaaaataa ggtctagtta ctgttttttt aaatgattaa
       61 taaataacaa ctcctttcta tgaaattgca aagcattcat cttcttttct gctaacgata
      121 tatccgtcaa tgcttgttac actttactaa aaagtatgag attgccgata tctggatagg
      181 tgtgctatac tacagaaaaa gatatacgat ctgccaccgg gtagtaaaaa agaggatgat
      241 cattccatta ggtctttgaa gggatgattc tcctcttttt gtgtattttt tatattgaag
      301 gaggaacgta tgtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg
      361 gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt
      421 gattggaatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgcctatgag
      481 tctctcttat cctatttgga cagggattgg cgcagttggt tcggtcatta ttggtgtggt
      541 cttgtttcat gataagctga atctttccac ttggttcttt gtcgggttgc tgctcatcag
      601 catcattggc atcaaaatca ctagcggtca ctagatactt agacaagcag acaaggactg
      661 atcagaaaaa gcggggcttt ttatttgata ttcctctttc tatgaaaaaa caagaaaata
      721 agactaatga cctagcgact tctctactca tcaggtgtat gcttgtacta tctatagaaa
      781 aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac tttggtcaca
      841 aacaagccct cagcgatgtc agttttagct tatctcctgg caaaatcgtt ggattagtag
      901 gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg atccattact
      961 cagaaggaac gatccgtatt ggtgaaaatg agatttctcc ctctagtcat aaaggattag
     1021 aacaagtagg agcattgatc gaatatccgg gtatctatcc attcttaaca ggttatgatc
     1081 atctgaagtt attctctgaa acaaacgatg tatctgctat cgacactatc gtgaaacagc
     1141 tcaagatgga aaaatacatt cacaaaaaag cgaaatccta ctcgctggga atgaaacaaa
     1201 aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta gatgagccga
     1261 tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc gacttggcta
     1321 ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa aaaatggtcg
     1381 atgagctgat cctgatcaat caaggaaaaa tcatcaaaca atgtacaatg gctgaattga
     1441 accaaacagc gcaagatttc atcgttatct ctacgacaaa tgatcaagct gctcgaacga
     1501 tcttgacaga cgctggatat cccttggaag atcctgataa aatcaagctt ctaaaaaaaa
     1561 acgaagagtt attaggaact gttatccagt tattgtctgc agaaaatgtc catgtgacag
     1621 atgtccagca tctgacgaat gatttggaat cttccctatt ggaaattctg gattcagtaa
     1681 agggggaaga agaaaaatga cgttgcttat aaaacaagaa ttatttaaat tgatcaagaa
     1741 aaagtcaact gctgttttat ctgttctatt agttgctttg ctcataggaa cagcacttct
     1801 agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc ttttcagtgc
     1861 cacatcttgg attgtcttta tcatgattgc tgctgcaagt acgatcattt caatggaatc
     1921 acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag aaatattagt
     1981 aagtaaatgg atcacattag ttatttattc tatttattta taccttttag ctattattgt
     2041 caccgtgctg atgaaactga tccttttccc ttcgatttcc tttacggaga agatttctac
     2101 tggtcagaca ctgatccaaa gcttattcac ttatactttg ggtagttata tcggtttatg
     2161 gctaatttta agtttagtgt tgatgattgc ttgttttatc aatagttccg gtgcctccat
     2221 ctcagcaggg atcgtctttt attttgcttc atcaatcata tctggtatcc tcatcgcact
     2281 cattcaaaaa tgggagtgga tcaaatggaa tccaatcagt atgctgaatc tgcaaaacca
     2341 aattggcaac gaagagatta tgaagcaatt gactcatctt tctactaatc aaatgctgtt
     2401 tggcaattta gcttacattg ctttgttctt agcattggga tacctcgtat tcaagcgaaa
     2461 aaatgtatga tggttcatta acaaagaagt tgtgacagaa gtggccagtt tcaagaaatt
     2521 tcggcttatt tttagaagaa gctacttctt gaacaccatt tattcaaaaa aagaagcgat
     2581 gacacgaatg ttgtcaccgc ttctttttt
//
LOCUS       DACIRY010000198         2597 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.207, whole genome shotgun sequence.
ACCESSION   DACIRY010000198 DACIRY010000000
VERSION     DACIRY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.207"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..587)
                     /locus_tag="IYE22_002692"
     CDS             complement(<1..587)
                     /locus_tag="IYE22_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507872.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
     gene            complement(735..1277)
                     /locus_tag="IYE22_002693"
     CDS             complement(735..1277)
                     /locus_tag="IYE22_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ9507873.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1412..1801
                     /locus_tag="IYE22_002694"
     CDS             1412..1801
                     /locus_tag="IYE22_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507874.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1805..2569
                     /locus_tag="IYE22_002695"
     CDS             1805..2569
                     /locus_tag="IYE22_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ9507875.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
BASE COUNT          902 a          435 c          499 g          761 t
ORIGIN      
        1 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
       61 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
      121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
      181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
      241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
      301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
      361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
      421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
      481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
      541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
      601 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
      661 atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt
      721 tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct
      781 gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg
      841 cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct
      901 tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc
      961 aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat
     1021 gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag
     1081 acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa
     1141 atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac
     1201 atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac
     1261 atttttggat caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa
     1321 atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa
     1381 tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc
     1441 cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc
     1501 aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt
     1561 ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa
     1621 tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg
     1681 cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca
     1741 gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta
     1801 acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt
     1861 tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac
     1921 aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat
     1981 cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta
     2041 tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt
     2101 agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat
     2161 gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg
     2221 ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta
     2281 tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt
     2341 gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa
     2401 aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa
     2461 agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga
     2521 agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa
     2581 aatcactcat gacgata
//
LOCUS       DACIRY010000199         2593 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.208, whole genome shotgun sequence.
ACCESSION   DACIRY010000199 DACIRY010000000
VERSION     DACIRY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.208"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1152
                     /locus_tag="IYE22_002696"
     CDS             <1..1152
                     /locus_tag="IYE22_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507876.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IYE22_002697"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IYE22_002697"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ9507877.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          859 a          491 c          620 g          623 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca act
//
LOCUS       DACIRY010000200         2558 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.209, whole genome shotgun sequence.
ACCESSION   DACIRY010000200 DACIRY010000000
VERSION     DACIRY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.209"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..901)
                     /locus_tag="IYE22_002698"
     CDS             complement(<1..901)
                     /locus_tag="IYE22_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ9507878.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="IYE22_002699"
     CDS             1184..2152
                     /locus_tag="IYE22_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9507879.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="IYE22_002700"
     CDS             2265..2543
                     /locus_tag="IYE22_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ9507880.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
BASE COUNT          837 a          465 c          524 g          732 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaa
//
LOCUS       DACIRY010000201         2523 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.212, whole genome shotgun sequence.
ACCESSION   DACIRY010000201 DACIRY010000000
VERSION     DACIRY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.212"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..450)
                     /locus_tag="IYE22_002701"
     CDS             complement(<1..450)
                     /locus_tag="IYE22_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9507881.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IYE22_002702"
     CDS             674..1465
                     /locus_tag="IYE22_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9507882.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IYE22_002703"
     CDS             1431..2384
                     /locus_tag="IYE22_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ9507883.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
BASE COUNT          886 a          418 c          402 g          817 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cga
//
LOCUS       DACIRY010000202         2483 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.213, whole genome shotgun sequence.
ACCESSION   DACIRY010000202 DACIRY010000000
VERSION     DACIRY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.213"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(1014..1304)
                     /locus_tag="IYE22_002704"
     CDS             complement(1014..1304)
                     /locus_tag="IYE22_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507884.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IYE22_002705"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IYE22_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACIRY010000203         2469 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.214, whole genome shotgun sequence.
ACCESSION   DACIRY010000203 DACIRY010000000
VERSION     DACIRY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.214"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..418
                     /locus_tag="IYE22_002706"
     CDS             <1..418
                     /locus_tag="IYE22_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9507885.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR"
                     /locus_tag="IYE22_002707"
     CDS             632..1327
                     /gene="vanR"
                     /locus_tag="IYE22_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ9507886.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="IYE22_002708"
     CDS             1305..2459
                     /locus_tag="IYE22_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ9507887.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          852 a          443 c          521 g          653 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtata
//
LOCUS       DACIRY010000204         2448 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.215, whole genome shotgun sequence.
ACCESSION   DACIRY010000204 DACIRY010000000
VERSION     DACIRY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.215"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..980)
                     /locus_tag="IYE22_002709"
     CDS             complement(<1..980)
                     /locus_tag="IYE22_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9507888.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTRE"
     gene            1463..1678
                     /locus_tag="IYE22_002710"
     CDS             1463..1678
                     /locus_tag="IYE22_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ9507889.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            complement(1919..2105)
                     /locus_tag="IYE22_002711"
                     /pseudo
     CDS             complement(1919..2105)
                     /locus_tag="IYE22_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          735 a          512 c          365 g          836 t
ORIGIN      
        1 tcttcccgtg ttcgactgta cacatccgca caactgattt taatgatatt cttcagctta
       61 ccaatattcc cactaaaggg atgagataac attagagaca aagcttcttt tgaaacaata
      121 atatcttttt gtaaaatatc cgcttcattt tgaaaaaata attgaatcag ctctaaccgt
      181 tctcgaatgg gacgatcttc aaaagttggc aacataacac tgatttgaat acgtcgatca
      241 aaggtgtcta ataaataatc ttcacttttt tccgtcgtgg cgaagataaa acgaacctta
      301 gcagaatgcc agtttacatt ttcaccgact ggacgataat ttccagtatc aataaaaaca
      361 aagagctttt cttgattttc tttcgacaaa cgatggacct catcaagaaa aagatagcca
      421 ttgttggctg ttgccaataa cccctctgta tccttctcag cccccgtaaa ggcccctttc
      481 ttgtatccaa acagtgttgc cgaaagcagt tcgggattgt ccgcataatc ggcacaattt
      541 aaaatagcaa atggtgcatc ttcttcaata atttgctcgt ggatagcgta ttcaaaaatt
      601 tttgtggcta aaaaactttt tccaaccccc gtggctccat ttattaatac actgagtccg
      661 ttaggcggat atttgacagc tgcaatacat ttttgaatga cctctctcag acttccgtca
      721 tgacccacca cagaatgaaa ggcttcgttt atttgctccg tattatctac aaccgcatat
      781 tcatctaaag gcttccacaa aacaggcttt cccggtaatt tctcgacctt tttatcttcc
      841 actaatctcg tcaaataatg gctggcattc tgacgagaga tacctagatt cttcgaaatt
      901 ttctcggcag tgacagaaga catattccct gttagtcgta tcagctcatc aaaaactcga
      961 tctttagcgc tctttttcat tttcccctcc agttcaacgt gtctagaagc ataataaact
     1021 atcggctaca atatgtctat tctatatttc tgttccaatt ttactttgaa acaaaaataa
     1081 tcgagcttaa actgatatta gttataaaac tatagttaga ttaccaatct tcctctcata
     1141 caataaactt ctctcacctg catccaacct acttatgtga aagaataact gccgattatt
     1201 tatctttttt acagttactt tcgaacataa aaaaatcacc ttcaaatact agaaaaacta
     1261 ctatttgatg gtggtttaga acaatttccg ttgattcagt atcgtctggt cccacatcct
     1321 ctgtctcatt aatcacaata gcatactcca aaggataaag ctggttgata tctcaatgcc
     1381 tttcacacct atcaattttg tcacacttat cttttcctag gttctattct atataatcta
     1441 gaaaaacaaa gaaggagaca ccatgaaaaa attcattatt ccttgtatat ttcttttctt
     1501 gagtattttg ttattttcaa cttccatcct agtcggttct tacctcgatt caagcggaat
     1561 gctgattgaa ccagcgttct tttgtgttcc gttgggctac ttaacattag gacttgcttt
     1621 ctttactgcc ttatacagtt tcgtcaaaca aagattattg aagaaatact cggcttgatt
     1681 tgcacgtaaa tcctctgttg aagaaaagtt agttttttca acaacgaacc ttcgagtatt
     1741 aagaatggat gttgatgtag acatcaaacg tctgcataaa cattcatttt attcctccat
     1801 taccaacaat tattcccaat cgccaaaaga agcttttttc ggaattacct tctccatttt
     1861 ttcacctgct ggttatattg tcttttgtaa taccgatagt cctcttcaat cattttcaat
     1921 atgcgaaaag aaccaatttt aacatggcac tcaaatcaca attcctaggg cggccaaaaa
     1981 gatacggctc gttgatttca agggtttcaa cgagttcatt gacaaaccca gcggtgctat
     2041 cttcttttgg aataagaagt tgtgatatct aaagtgagtt gattcatgtt ataatttgta
     2101 tgcatgattt tgatcctttc aagggttgaa tgatttagag tgcgagtatc ctctcgtact
     2161 cttttatttt acctaaaaag ctccagaaat gacgaatgac tcgtcatttc tagagctttt
     2221 ttcagagaat tatgtcacaa tctctttttg ttatattgat attctatatc tttttttatt
     2281 ggttttattc ccttgttcgt tatagactaa aatccttctt tcagcattat tcaaatcaaa
     2341 tggcaacata atagagaaat ttatagtatg tataatcatc tgaattcaat caaatcaacc
     2401 ggagtcatat acggagtcaa actatcccac tgagagttta ttaaacgc
//
LOCUS       DACIRY010000205         2441 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.216, whole genome shotgun sequence.
ACCESSION   DACIRY010000205 DACIRY010000000
VERSION     DACIRY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.216"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            297..551
                     /locus_tag="IYE22_002712"
     CDS             297..551
                     /locus_tag="IYE22_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507890.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            621..866
                     /locus_tag="IYE22_002713"
     CDS             621..866
                     /locus_tag="IYE22_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9507891.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            942..1307
                     /locus_tag="IYE22_002714"
     CDS             942..1307
                     /locus_tag="IYE22_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507892.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1368..1931)
                     /locus_tag="IYE22_002715"
     CDS             complement(1368..1931)
                     /locus_tag="IYE22_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507893.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          723 a          440 c          384 g          894 t
ORIGIN      
        1 aacaaaagta tttgttaaat atgtttccat tcgctatggg atcatcattc tttccttttt
       61 agtgaatgcg atcgtcctct ttttatatgt attgaattac ttagatatca atccattgat
      121 tttttctcga tagataagac acattcagcg gaacagttcc tgcttatcag cagaaaactg
      181 ttctgctttt tcatttttca ttgatctatt cttcttttat tttcctagca tttcatttgt
      241 gatgtatgca ataacatgtt atcttttatt atatgaatcg ttgaggagtc cgttacatga
      301 ttacgttggt gagagttttg ttttgggtgc ctgcggtcgc actggtcgca agtattgttt
      361 atcttatgaa ttggaacaaa gaacggtttt atttagctat tttgactctt ccggctatct
      421 actttatgtg gaaagttttt aattacaact actttgaacc cgatagtgtt tttatagaag
      481 aattatcagg actggttttg tctttattga tcgtcattct ttatttgata cgtttaaata
      541 aaaaacattg agtactattg attgcatgtg tagctttcta tagtcattat aactattcta
      601 ctataatgaa aggtgattaa atgagtatcg aagaagctat tttactggat actttacgtt
      661 gtcgtctgaa tttcttaaga actggccgat ctttatctca agtagctgtt tctagcgcgt
      721 taagtattcc tttattcaaa tatcgtaagt acgaagaagg agaagaaatt ccaagcatca
      781 ttgaattaat ctatatcgct gacttttacg atatttccgt tgattaccta attggtcgct
      841 cagaaacagc aagcaaactg aagtgatttt ttccagcctt ggtgctggtt ttttcattta
      901 acgtctaagg ttaacaatct atatataaag gagacatttc tatgcgatta tggcatgaaa
      961 aattgatttc aaagcttcct cgtcaacagc ttttaggaca acatcgggaa tgctgtgcat
     1021 taagaggaaa tggctgggga aagaaacatg ctactgtgga ttatgttttt cactattctc
     1081 cttatagatt attccaatat catcagcttg tcatggatga aatggaaaaa cgtggttaca
     1141 gcccagcacc agagtggaaa aatcctatgt atcgtggcaa atcatgcgaa gcattttttg
     1201 atttacctac tgtagagcgc aacgtgccga tttatccgga acacgatgaa gtttatctag
     1261 cagaatgttt agcaaatttg gagcaaaaag gaatttatct tacctaatat aactagaaaa
     1321 gccttctatc tcctcttact tcattaaaaa gctttaacca tcttagatca agctaaaatg
     1381 tcgtaagttt tagggttatc ttcatcctca cgtgcaaaac taaaagcgta ctctagtttt
     1441 ggttcatcgc caacttccct agacataaat gaacatctcc tgacgtggtt taaaagtacc
     1501 gcatcttcga cgtctgtata cgatgcacag cacagattgc ttcttttgat ctttcttttg
     1561 atttttctgt tgcttcttct acaccttctt cgattactac atcgatgctt acaattgctt
     1621 ctgcctaata ttcccatgca tcgtcgattt aagcctctgt agtcactgtg atcgtttcca
     1681 cattttaact cctctgtcgt ggcttcagtt gattttgtga tcagttcagt tgctaagcca
     1741 gagtcagaac aacctgccat tgctagcgtt aatattgata gtaaaaatag tttttttatc
     1801 ataaggttcc ccccctgaaa atagactaaa ataataacgc tttctttttt tgttttcaga
     1861 cgaaaattaa taatttctaa atattcttta aaaaagctta taactattat gcctttttta
     1921 atatataaca cagttatttt taaaaataag aatgctaaat cactctacaa taacggcgaa
     1981 agcacaaaaa ataaaaaaat tattacgtta ttaaaaaaac agatgaaaga tcaggtttta
     2041 ttagatctca ttctttcatc acttgaaggg atttaatgaa ctactttttc ctctttgtcg
     2101 tttttatata ttcgtattcc ttcaaacagt aggatagcct ctttcaatgc ttgatatttg
     2161 aactgtccgt tacttaaaaa tttagatgtg ccgccctctt cctagaacat attctttttt
     2221 actttttcat taattgacag accaatcgaa cgaaactgta aatttcgata ggataatgat
     2281 tggtcaaact cattcatttt caagacatct cgattcatat tcaagatagg aatcttttta
     2341 tcaacatcac tttattcgta ttttcctcta ccataaaagc ttcacgctcc acaaaattgt
     2401 tttatacact actattcagc ccattcaacg accaatgaat t
//
LOCUS       DACIRY010000206         2409 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.217, whole genome shotgun sequence.
ACCESSION   DACIRY010000206 DACIRY010000000
VERSION     DACIRY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.217"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            117..2270
                     /locus_tag="IYE22_002716"
     CDS             117..2270
                     /locus_tag="IYE22_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ9507894.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          818 a          424 c          535 g          632 t
ORIGIN      
        1 gttgatttta tcttgaaatt aggtaaagaa tttcttgctc cgcccatttt ttctttttta
       61 ttaagaagtt tttgggtaca atgaagcatg aatgtaaata ggcggaagga taggaaatga
      121 tgaaagattt tatgaaaaaa atattaaata cttcttggtt agaaaaaata agaaaaggtg
      181 cacaagaacc taagaacaaa acgtttcgcc ggtcccatgt tccttttcgc ttaaattttc
      241 tttttttcat tatatttacg ttatttgttg cgttgattgc tagattgggt tatttgcaga
      301 tcgtgaacgg cgaagagatg gaggcgcgag tgaagtctac ttctacgatt acggtccaag
      361 aaagtactcc tcgcggaatg atctacgact ccacaggaaa agctttagtt acgaataaag
      421 cgaatcaggc catcacatat acgagaggaa cacaagttac ggctaaagat ttgcttgaaa
      481 tcgcaacgaa gctgaactcg ctgatcgatg ttccggtaga tgaaaatctg acagatagag
      541 ataaaaaaga tttttggctg gctgatccca aacatttgaa agaagccaca aaaagacttt
      601 cggcaaaaga aaagcaatta gggaacagcg aacaatatag tgcgatggtc aataaagtca
      661 cagctgatga gattaattta aacgaggagc aattaaaaat tgctaccatc tttaaacgga
      721 tgaatagtgc atatgaatta aacactgtct atatcaaaaa ctctggtgtt actgatcaag
      781 agttagctgt tgtggcagaa cgcgcttctg aacttccagg agtttcgact ggcacagact
      841 ggacacgaga atataatgca gcaagttcac tgaaaagtat tcttggcagt gtgacgactg
      901 aaaagcaagg acttccagca gatgaagcag agaaatacct agcaaaaggt tattctcgga
      961 atgaccgtgt aggacaaagt tatttggaaa aacaatatga agatgtcctg caaggaacca
     1021 aaacccaata tgaagtttcc cttgataatg aaggaaatgt ttcgaatcaa aaagaaatct
     1081 tctctgggga aaaaggttct aatttaatgt tatctatgaa tgcagaattc caatcaaaag
     1141 tagaagaaat tttgaaaagg aattatcaaa cattgatcaa caacggcaaa gcacagtatt
     1201 cgcctggtgc ttatgcagtg gctatgaatc ctcagacagg agaagtgcta gcaatgactg
     1261 gtttcagtca tgaacaaggt tccaaagaaa tcaccgagaa tgctttaggc acgattacta
     1321 gcgcctttga tccagggtct gtagtcaagg ctggtacttt gacagcaggc tgggcaagta
     1381 acgcgatcag cggaaatcaa gttttgattg atgagccgat tcgtctgcaa ggtgcttcag
     1441 aaaaaagctc tgtctttaat cgaagcggac aagttgctct tgatgcagtc aaagcatcgg
     1501 aattgtcttc taatacgtat atgatcaaag tggcgttaaa gatgttagga ctagactata
     1561 cacctggaat gggactacct agtttagatg aagaagctaa agcttatcag caattgagag
     1621 actctttcaa agagtttggt cttggtacaa cgactgggat cgatttgccg aatgaatctc
     1681 ctgggatctc acgttctgtt gattatatga agaagttcaa tgcggataat ggcaaagaat
     1741 ggtatgctcc aggtaacttt accgatttgg catttggaca gtttgacaca tatacaccaa
     1801 tccaattagc tcaatatgct tcaacagtcg ctaatggcgg gaaacgagta cagccgcacc
     1861 tagtcaaagc gatctatggc aatgacgaaa atggaaactt gggtgaagtg aagaaagaaa
     1921 ttggcacaac agtagaaaat acggtcaata tttctgcaga aaatatggcg atcctccgtg
     1981 aaggattcca ccaagttgtc catggtaccg atccttacac aacagccaaa ccattagcca
     2041 gtgcgaaaat ggacctttcc gcaaaaacag ggactgcaga aacggttgcg gaaggtcatc
     2101 cagatattgc gacagtcaat agtaatatcg tggcatacgg accaacagac aatccggaaa
     2161 ttgccatcag tgttgtcttg ccgaacttat tagacgaaca agaccacata aacttgacga
     2221 ttgctaaaga aataatggac gcgtattatg atatgttcat ggcaaaataa aaaagaggtc
     2281 gtgaaaaagc cgcttagttt cgagtatgaa gaaaaatttt gtgaaaatag cttctcacat
     2341 ttttgcttaa atttgacttc ataccgagaa actggctttt gaacaccgtt tactcggcaa
     2401 aaaaggttg
//
LOCUS       DACIRY010000207         2398 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.218, whole genome shotgun sequence.
ACCESSION   DACIRY010000207 DACIRY010000000
VERSION     DACIRY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.218"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            207..2069
                     /locus_tag="IYE22_002717"
     CDS             207..2069
                     /locus_tag="IYE22_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9507895.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IYE22_002718"
     CDS             2069..>2398
                     /locus_tag="IYE22_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9507896.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACIRY010000208         2373 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.219, whole genome shotgun sequence.
ACCESSION   DACIRY010000208 DACIRY010000000
VERSION     DACIRY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.219"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(36..992)
                     /locus_tag="IYE22_002719"
     CDS             complement(36..992)
                     /locus_tag="IYE22_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507897.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1117..2295)
                     /locus_tag="IYE22_002720"
     CDS             complement(1117..2295)
                     /locus_tag="IYE22_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ9507898.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT          711 a          392 c          405 g          865 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca acttgggagc gtcaataatt
     1081 ttgtgtaaat aaattgtcct cctgcaaaat aattagttac tcagtaaaca ttgaaactaa
     1141 tgtatcggtt acctgttgaa aacctttatg gcttctgttt agaaattttt gattgtatgt
     1201 atcaaaaatg ctgactagaa agcgttctag tgattcttca ttttgaaact gctcttttct
     1261 acggctgtat cttttaattt gcttattgaa agactcgatt agattggttg agtaaatggt
     1321 tctacgaatg ctaggtggaa aatcataaaa agttaataag tcttggtttt ctatgagtga
     1381 ctgcgtcact ttaggatagt ttttcttcca tttctcaatc atgccggata agaaggtatt
     1441 cgcttcttct tttgagttag cttgataaac agccttaaag tcatcacaga tttcttttcg
     1501 gtctttgaca cgtactttat gagcgatgtt acgagataca tggatacaac aatgctgata
     1561 ttttgcttta ggataaattt gatggatagt atctttcatg ccttttaagc cgtccgtaat
     1621 aaaaagcaag acttcttgaa ctcctctgga gttaatatcc tgtagcagct cattccaaac
     1681 gtatgttgat tcagttggag caatcgcata actcagtact tctttagtgc cgtcttctcg
     1741 tataccaatg gcaatataaa tcgcttcttt ggatacggtt tgacgtttta gtggaatgta
     1801 agtagcgtcc ataaaaatag cgacatactt atcatttaag gctctggatt taaaggcatt
     1861 tacttcttca gtcagaactt tagtcatgtt ggacatggtt tgtggagtat agtgatgacc
     1921 gtacattttt tcgatcaaat cagcaatttc agacatcgta acaccttttt cgaataaatg
     1981 gataatagtg gtttccaatg tatcgtttgt tcttttgtag gctggtaaag tttgttgttt
     2041 aaactcacca ttacgatctc taggtatttc caatgttaat tcaccatatt cggttttgat
     2101 tgatcgaaag taagaaccgt ttctcgaatt acctgaatta aaaccagtgc gatcatattt
     2161 ttcgtaatct aaaaaagccg ttaattcagt ccgtaggagt gtgtttatcg ctttttctaa
     2221 gtgcgaacgg aataattcat ttaaatcgcc tttagtgact agagtttgca caatttctgt
     2281 agtaaaatca ttcataggga agtcctcttt tctgtgaatt ggttgtcgtt aactttattc
     2341 tacagaaggg acttcctttt ttgtatggat ttt
//
LOCUS       DACIRY010000209         2365 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.220, whole genome shotgun sequence.
ACCESSION   DACIRY010000209 DACIRY010000000
VERSION     DACIRY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.220"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            150..1451
                     /locus_tag="IYE22_002721"
     CDS             150..1451
                     /locus_tag="IYE22_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ9507899.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="IYE22_002722"
     CDS             complement(1555..1755)
                     /locus_tag="IYE22_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9507900.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          769 a          454 c          407 g          735 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaaatcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacacaa ggataatgca
     1921 atgaaatata aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc
     1981 gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta
     2041 aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta
     2101 tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct
     2161 ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt
     2221 ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag
     2281 aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc gttcttctct
     2341 ttaaacgcat ctactggtag ataac
//
LOCUS       DACIRY010000210         2351 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.221, whole genome shotgun sequence.
ACCESSION   DACIRY010000210 DACIRY010000000
VERSION     DACIRY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.221"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..544
                     /locus_tag="IYE22_002723"
     CDS             <1..544
                     /locus_tag="IYE22_002723"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9507901.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IYE22_002724"
     CDS             complement(1017..1589)
                     /locus_tag="IYE22_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9507902.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IYE22_002725"
     CDS             complement(1605..2210)
                     /locus_tag="IYE22_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ9507903.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          469 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DACIRY010000211         2034 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.222, whole genome shotgun sequence.
ACCESSION   DACIRY010000211 DACIRY010000000
VERSION     DACIRY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.222"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IYE22_002726"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IYE22_002726"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ9507904.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IYE22_002727"
     CDS             complement(1431..1823)
                     /locus_tag="IYE22_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9507905.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACIRY010000212         2026 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.223, whole genome shotgun sequence.
ACCESSION   DACIRY010000212 DACIRY010000000
VERSION     DACIRY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.223"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(310..807)
                     /locus_tag="IYE22_002728"
     CDS             complement(310..807)
                     /locus_tag="IYE22_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9507906.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IYE22_002729"
     CDS             complement(847..1656)
                     /locus_tag="IYE22_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9507907.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1930..>2026
                     /locus_tag="IYE22_002730"
     CDS             1930..>2026
                     /locus_tag="IYE22_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ9507908.1"
                     /translation="MRNILDVEQVRKRLEKGVILMEKRYGVVPVVT"
BASE COUNT          606 a          356 c          359 g          705 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactg
//
LOCUS       DACIRY010000213         1949 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.224, whole genome shotgun sequence.
ACCESSION   DACIRY010000213 DACIRY010000000
VERSION     DACIRY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.224"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(330..403)
                     /locus_tag="IYE22_002731"
     tRNA            complement(330..403)
                     /locus_tag="IYE22_002731"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(367..369),aa:Ile,seq:cat)
     gene            complement(592..1611)
                     /locus_tag="IYE22_002732"
     CDS             complement(592..1611)
                     /locus_tag="IYE22_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ9507909.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1622..1828)
                     /locus_tag="IYE22_002733"
     CDS             complement(1622..1828)
                     /locus_tag="IYE22_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ9507910.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          575 a          396 c          328 g          650 t
ORIGIN      
        1 actttttttc aaaaatatgg tatgcctttt aatagcttca aatataaaag agtttaaagc
       61 gtaacacact tatggggaag tggcttgggg tgcgctttaa actcttcttt attatcatct
      121 cacaatttaa cccaaatgtc tttctattta aaaatcaaag taaaactttt caaatataca
      181 gaaatataac tatgtgaaac atcctttcat taatccataa aaggatacat aaaaaagcca
      241 ctcatttgag tggcaatgaa gaaaagcttt agcttgtata atactcttca aaaaattcta
      301 acacagaacg attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg
      361 acggttatga gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa
      421 agccataaac aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga
      481 gtgtgactat tatgggtgat agtgaagata tgcgataaca tcactatttt atcgaatgat
      541 ttttatagtt gtcaatatag ttatgtaatg ctcctcaacg aggagctatt tttatcgttt
      601 aggaatattt aaataccaac gtttgtcatg gaaatcttgc gcaccgcctt tagtgtttcc
      661 ctctggatca ttcgttgccc gcatcatgac atagactttc ttattaggga agttacgcat
      721 gttaaaagat acatgatagc caacatttcc agaagtatta taagcttgat ttacatctga
      781 tctataaatt ccatcagctc ttactcgagc taattctttc ccagtattgt aatccataat
      841 gaagatatac tcgtatttat agttagcaat gtgccatcca gctacatgca aatttgcatt
      901 ttcgatttct ccaaactgat caatgtgggc gtaattcgtt ccatctgtca gtgtaggatt
      961 agctgcacca gctcgtgttg gatcaatgac aggcttgttt tcagaagttg ttggattttc
     1021 atcggtaaat ccatgagcta aatcatatgc taatttttct ttacttacgc ccatttcaga
     1081 aagataaccg taaggatctg tatgatcgcc ccagatgttt tgtgttaccc ataaatgcga
     1141 tttgattccc ggttggttat aaggagtgtc caacgttaat ggaataccat atttcattgc
     1201 tgaatctcta gccaattcaa cgtatgcctt gtagtttttc tcaaacgttg ctttatcatg
     1261 tgtgtgttgt aactcaatct gcacaggact gttggcatta gcatacgaac cagcaccgta
     1321 ctgtacataa ccaggttgac cgacttgata aacaattccg ccgtctccca caatataagc
     1381 agtataagcg ctagtccatg aacgttgcat atactgcgct tcattgcgtc ctgttgctgt
     1441 ttcattagcc gtttcatgca gtaaaatata ctgattattt gctacttgtg agctaccttc
     1501 atttgcgccc aaattaaatt cattgttgat agtataggca aatccgttaa ttggcaataa
     1561 aaaaagagcc gttaacaggc tcatcgcagt aatagtaatt ttctttttca tatctatcct
     1621 cctatttatc tgatgaaatt ttcttatacg ttcgatttga tacacctaaa atactcccta
     1681 gaaatgctcc aaaacctgta ataatcatca ccgttatatc tgtgtaccgc cagttaacac
     1741 cttttccgac aagaccgaca aaagtcgcta aagcggggat aactactata gcgatccatt
     1801 tcaaaatatc aaaggttcta ttgttcatac tatccacctc ccttcattgg ttgggctact
     1861 ttctttttca atcgcttgtt ttcttctatc aatatttctc tttctgaaag caaggcatca
     1921 atttgcccgt ccagttgttc aattttatc
//
LOCUS       DACIRY010000214         1875 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.225, whole genome shotgun sequence.
ACCESSION   DACIRY010000214 DACIRY010000000
VERSION     DACIRY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.225"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..423)
                     /locus_tag="IYE22_002734"
     CDS             complement(<1..423)
                     /locus_tag="IYE22_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ9507911.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(532..1563)
                     /locus_tag="IYE22_002735"
     CDS             complement(532..1563)
                     /locus_tag="IYE22_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ9507912.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          577 a          316 c          323 g          659 t
ORIGIN      
        1 aatctgtttt gattttgttc catttgaaat ttctctcact cgcttttttg tctcatcatc
       61 aaataaaatg aaagggtctt ctaaacgatc gataaatact tccattgttg ttgttggtag
      121 atgataagaa gtcatgacag gcactgcacc aaggtaagac acagaaacag ctagaaggta
      181 tgtgtcaaaa gcggaactct tataaataat aactttgtct ccggatttca ctccaagatg
      241 agccaattga taagcccgtt tcaacacact ttcgcagcat tttttgtaag ttgtttcaag
      301 tcctaattct gggaaagctg aaagtggctc atcaaagata atcggggtct cggaaaagtt
      361 agctgcagct tcttgaaaat tcgtaaataa gttcaaaggg gaatacgtta tggtctccat
      421 cattcacact ccattatctt ttgttaagta atatacaaat tcaagataat cgtaacataa
      481 tcctttatat aataacatag tttattgtga aaaaaagatc atacatttgt tttatatgga
      541 agaaacccga acgaatgccg ctaattcgtg ataagaaaaa tcaaccatct tttcccagat
      601 atcttgaaaa gaatgggatg agaggtatcg attaccacat aaataattta ccgcgtcagc
      661 atcatctaaa tatagttctg cataataacg atatgttttt ctgcatagtt ctggcgatac
      721 gtttttatat actgtaattt tgctcaaaat ttggcgaata gtccgaggag aaatttgctg
      781 atcatgtgag gaacgaaaaa caaaagaatt ttcgtccgta ttttgtgctg ttaacttaaa
      841 atagttctcc agaaatggca tcgcttcttt tggtattggt actttattca gtctgttttc
      901 ttttctattc agattaagaa tctgcttggt caaagataca tctttacgtt taagttctac
      961 tacttcatgg atctttaatc cgcaatacag aagaatggca gtaattgcaa ggttcctcgg
     1021 tctattcttt tcttcaaagc tagcttctct gcttgtttga aagccttcaa tcgggataaa
     1081 caaatgctca aaatctttga aaagttctgc atcgatatat cgcggagctg ctttggttgt
     1141 ttccggtaaa cttgtaccca aaagagaaat ctcttcttca atcagtcctt tttgatacaa
     1201 aaatcgtaaa aaatgattta atgaatattg cttccttgaa atcccgtaat agctattttc
     1261 aaacaattgc tccgtttctt ggttcgctgt agtttggaac gaacgagtat gtcgatagat
     1321 tttagctaag aaattctgat aatcttgttt ttctaaatgg ataatcagct gatcatccac
     1381 ttcttcagaa aatacttttc tcaaataatc agtaaacaga tgaaaatctt ttgcgtattc
     1441 atagcgactg cctaatgaaa gggaagaacg attttcttct aaaaactgtt gaaagacgga
     1501 agggagcaag tgtgtacgtt gctttaactt ttcttgcagt tgttttctag ttttgctgtc
     1561 cattacaaat tccctccaat agtgttcatt ttctatattt taccgcattt catcaaatga
     1621 agatagacag tttgtcttga tcttaggaag caaattttta actaagtact agctaaaaga
     1681 tatgaagtaa gaagatacat ttttagttat tttattagtg cagtcattct tttgatcttt
     1741 taattgattt atcctctttt tagctatgtg gttacttcat gagcaatgaa atggatttgg
     1801 ttcgttctaa tggcaggagg ctctttcttt tatttccgaa aaattagaaa acattttttc
     1861 agaatcgatt ggctc
//
LOCUS       DACIRY010000215         1753 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.226, whole genome shotgun sequence.
ACCESSION   DACIRY010000215 DACIRY010000000
VERSION     DACIRY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.226"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            266..1750
                     /gene="guaB"
                     /locus_tag="IYE22_002736"
     CDS             266..1750
                     /gene="guaB"
                     /locus_tag="IYE22_002736"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ9507913.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          596 a          308 c          399 g          450 t
ORIGIN      
        1 cctctccact aaacggtgtt caaaagtttg catctgcggt aataaaccca accaattcaa
       61 aaacatgaga agctgttttt gattggctgt tcttattact tgatgcagga cagctttttt
      121 acaatctctt ttaatataca cagatgtaaa cgctttatat tggtattcta tgagaaagct
      181 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      241 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      301 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      361 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      421 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      481 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      541 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      601 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      661 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      721 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      781 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      841 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      901 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      961 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
     1021 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
     1081 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1141 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1201 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1261 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1321 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1381 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1441 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1501 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1561 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1621 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1681 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1741 tgaatcttaa aaa
//
LOCUS       DACIRY010000216         1685 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.227, whole genome shotgun sequence.
ACCESSION   DACIRY010000216 DACIRY010000000
VERSION     DACIRY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.227"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(141..1433)
                     /locus_tag="IYE22_002737"
     CDS             complement(141..1433)
                     /locus_tag="IYE22_002737"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ9507914.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          483 a          351 c          295 g          556 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca ttgtatcaat aatagataca tatttcaaat
     1501 aaaatcgaat gatttttatt tttattttat aaaaacgctt aaaaaacgaa ctttatcttt
     1561 ttagaatggt tattaaaaaa gaggttgtaa cagaagtggt cagcttcaag aaataagaag
     1621 gaatgtacga aaattgcttc tcaaattttt gtgaaatttc gacttatttt tcgaagaagc
     1681 tactt
//
LOCUS       DACIRY010000217         1672 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.228, whole genome shotgun sequence.
ACCESSION   DACIRY010000217 DACIRY010000000
VERSION     DACIRY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.228"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            301..1245
                     /locus_tag="IYE22_002738"
     CDS             301..1245
                     /locus_tag="IYE22_002738"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ9507915.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          592 a          237 c          322 g          521 t
ORIGIN      
        1 attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct
       61 gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt
      121 tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga
      181 attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa
      241 taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc
      301 atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc
      361 gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa
      421 gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat
      481 gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca
      541 gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca
      601 atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt
      661 gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca
      721 tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca
      781 ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat
      841 tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta
      901 gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat
      961 gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact
     1021 gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat
     1081 ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa
     1141 aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc
     1201 aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg
     1261 aactaaaaat cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat
     1321 atctaatagg taagcaaata aacagaatgg ttaaaattaa tcggtccgaa aatgtatccg
     1381 tacatatgaa catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt
     1441 tatctgtggt taattaaaaa atccttccgc aacggcaaat acaatgattc atatctaatt
     1501 cgttcatatt tgattaactg aataaagaaa ctggttttta tcattcaatt aaacgacaaa
     1561 aaaagtaaga aaaactgcaa aattttttta taattttgca taagcagatt ttgatggaaa
     1621 aatgacgatt tttgataaaa aaccgttcga gagaggttta tcatttttta aa
//
LOCUS       DACIRY010000218         1636 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.229, whole genome shotgun sequence.
ACCESSION   DACIRY010000218 DACIRY010000000
VERSION     DACIRY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.229"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(183..1502)
                     /locus_tag="IYE22_002739"
     CDS             complement(183..1502)
                     /locus_tag="IYE22_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ9507916.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DACIRY010000219         1593 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.231, whole genome shotgun sequence.
ACCESSION   DACIRY010000219 DACIRY010000000
VERSION     DACIRY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.231"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            44..250
                     /locus_tag="IYE22_002740"
     CDS             44..250
                     /locus_tag="IYE22_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507917.1"
                     /translation="MKQKVIGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(612..851)
                     /locus_tag="IYE22_002741"
     CDS             complement(612..851)
                     /locus_tag="IYE22_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507918.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1137..1451
                     /locus_tag="IYE22_002742"
     CDS             1137..1451
                     /locus_tag="IYE22_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507919.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          544 a          218 c          279 g          552 t
ORIGIN      
        1 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtgat
       61 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
      121 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
      181 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
      241 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
      301 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
      361 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
      421 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
      481 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
      541 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
      601 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
      661 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
      721 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
      781 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
      841 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
      901 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
      961 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
     1021 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
     1081 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tagcttttgg
     1141 atttttacag taattttatc ttaataatag caatattatt acttctaaat atttggtttt
     1201 ttgataagag tagaaatgca gggataggtt ttagaaccaa acgaagtacg tcttctgaaa
     1261 aaaagtgggt atattcacag actatttttt acggaggggt tatttcaatc agtttacttt
     1321 cttcaacact ttattctttt aatgttatag acgtttccat gtcaaatttt atatctatta
     1381 ttggaatcct tatttctgca attattacac aactacttct tgtgtttgaa gagaagtcaa
     1441 aaaataatta aagcaattat gccttttagt acaggaatta ttacttctta caaatgtata
     1501 gcgaatgtga agcctgctgt tttaattgtt ctagaaaaac ttttgagtga acaattattt
     1561 tatgttagga tgaattaatt tctctagagt tta
//
LOCUS       DACIRY010000220         1535 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.232, whole genome shotgun sequence.
ACCESSION   DACIRY010000220 DACIRY010000000
VERSION     DACIRY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.232"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(186..>1535)
                     /locus_tag="IYE22_002743"
     rRNA            complement(186..>1535)
                     /locus_tag="IYE22_002743"
                     /product="16S ribosomal RNA"
BASE COUNT          344 a          451 c          341 g          399 t
ORIGIN      
        1 tatggccgtc cgcctatttt gcttcaggct aaacaatttg ttcagctttc aaattcaacg
       61 cggtgttctc ggtttgtatt acttacattt acttcaaata tccagttttc aatgaacaaa
      121 agtgtttgag agtaaacctc tcaaaactga acaaagtaaa aacaaattgt gtagtctccg
      181 taatattcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta ccttgttacg
      241 acttcacccc aatcatctat cccaccttag gcgactggct ccaaaaggtt acctcaccga
      301 cttcgggtgt tacaaactct cgtggtgtga cgggcggtgt gtacaaggcc cgggaacgta
      361 ttcaccgcgg cgtgctgatc cgcgattact agcgattccg gcttcatgca ggcgagttgc
      421 agcctgcaat ccgaactgag agaagcttta agagattagc ttagcctcgc gacttcgcaa
      481 ctcgttgtac ttcccattgt agcacgtgtg tagcccaggt cataaggggc atgatgattt
      541 gacgtcatcc ccaccttcct ccggtttgtc accggcagtc ttgctagagt gcccaactga
      601 atgatggcaa ctaacaataa gggttgcgct cgttgcggga cttaacccaa catctcacga
      661 cacgagctga cgacaaccat gcaccacctg tcactttgcc cccgaagggg aagctctatc
      721 tctagagtgg tcaaaggatg tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa
      781 ccacatgctc caccgcttgt gcgggccccc gtcaattcct ttgagtttca accttgcggt
      841 cgtactcccc aggcggagtg cttaatgcgt tagctgcagc actgaagggc ggaaaccctc
      901 caacacttag cactcatcgt ttacggcgtg gactaccagg gtatctaatc ctgtttgctc
      961 cccacgcttt cgagcctcag cgtcagttac agaccagaga gccgccttcg ccactggtgt
     1021 tcctccatat atctacgcat ttcaccgcta cacatggaat tccactctcc tcttctgcac
     1081 tcaagtctcc cagtttccaa tgaccctccc cggttgagcc gggggctttc acatcagact
     1141 taagaaaccg cctgcgctcg ctttacgccc aataaatccg gacaacgctt gccacctacg
     1201 tattaccgcg gctgctggca cgtagttagc cgtggctttc tggttagata ccgtcaaggg
     1261 atgaacagtt actctcatcc ttgttcttct ctaacaacag agttttacga tccgaaaacc
     1321 ttcttcactc acgcggcgtt gctcggtcag actttcgtcc attgccgaag attccctact
     1381 gctgcctccc gtaggagttt gggccgtgtc tcagtcccaa tgtggccgat caccctctca
     1441 ggtcggctat gcatcgtggc cttggtgagc cgttacctca ccaactagct aatgcaccgc
     1501 gggtccatcc atcagcgaca cccgaaagcg ccttt
//
LOCUS       DACIRY010000221         1514 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.234, whole genome shotgun sequence.
ACCESSION   DACIRY010000221 DACIRY010000000
VERSION     DACIRY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.234"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            270..1400
                     /locus_tag="IYE22_002744"
     CDS             270..1400
                     /locus_tag="IYE22_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ9507920.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          426 a          247 c          329 g          512 t
ORIGIN      
        1 cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg ttggattaag gctttttcca
       61 atagctaaga atgttttctt tacgttgtat agtgctaaaa ctcggacgat gagatgatgc
      121 taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc tttatatttt tgtgactgac
      181 ttatttgatg agacaaactc gctcctcacc tgacaagtgg attgctgtgt gaggaggaga
      241 tgcggtgaaa aaagatcgat tggtggattt tgggtcctta tttgactttg tccatgatcg
      301 gtcttttaga agtatacagt gccagttctt atcgtttatt gcaagccgat gaaaatacaa
      361 aaagtctatt attgcgtcaa ctaattttca tatttttgag ttggagcgtg atcttcttag
      421 ctcgttcagt caaactacac tatttacttc accctaaaat agcaggatac ggtttagcct
      481 tatcgatttt ctttttagta ttagtaagaa tagggatatt cggtgtcact gtcaacggcg
      541 cacaacgttg gatctctctg tttggcattc aattccagcc ttctgaactg gcaaatcttt
      601 ttttgatttt ttatttaagc tggttttttc gtgacggaaa tagtagccca aaagatctaa
      661 aaaaaccatt cctgattaca gtaggtataa cttttctgat tttatttcag ccaaagattg
      721 ctggagcatt gatgatcctt tcgattgcgt gggtcatatt ttgggcagcg gcggttccat
      781 ttaaaaaagg gatctatcta atcgttactt tttctgcatt gctgattgga gcagcaggcg
      841 gggtattata tttaggaaat aaaggttggc ttccacagat gtttaatcat gcttatgaaa
      901 ggatcgcgac tttaagagat tcttttatcg atagtcatgg tgcgggctat caaatgaccc
      961 attcgtttta tgcactatat aatggtggca tatttgggcg gggattagga aatagtatca
     1021 caaaaaaagg gtatcttcca gagacagaaa cagattttat tttttcgatt attacagaag
     1081 agcttgggct aatcggtgca ttgtgtgtgc ttttcttatt gttctcatta tgcatgagaa
     1141 tcttttgttt aagcagtcgc tgtaaaaatc agcaagctgg attgtttctt ttaggattcg
     1201 gaactttgct attcgttcag acgataatga acgtgggaag tatcgcgggg ctgatgccaa
     1261 tgactggtgt gccattgcca tttgttagct acggaggaac aagttatctg atcctttctt
     1321 taggaatcgg aatcacgttg aatatttctt caaagataca ggccgaagag ttggcgctct
     1381 atcgacctga aaaacaataa agagaaactg agaaactctc agtttgaatt atcataaagt
     1441 acaatctttt atttttcaag cgagaggttc ttttagaaag aggaattcgc aatgaaagta
     1501 tctcaaaaaa tcat
//
LOCUS       DACIRY010000222         1498 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.235, whole genome shotgun sequence.
ACCESSION   DACIRY010000222 DACIRY010000000
VERSION     DACIRY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.235"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            369..1355
                     /locus_tag="IYE22_002745"
     CDS             369..1355
                     /locus_tag="IYE22_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ9507921.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTYDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT          572 a          223 c          305 g          398 t
ORIGIN      
        1 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
       61 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt ttgcttattg
      121 atggattgga taaattaaac catcacaacg agtggaaatt atatgaaatt tttcataaaa
      181 attcatatag aaacaaatga cattttatta gaatatgact ttcattaaca taaatgtttt
      241 caaatttcaa ttaaattgtt ttaatcaaat tccaatctaa atatttgtag aaaacaatcg
      301 tctcctatag aataaattta taagtaacaa tataccaatc ataaatctat ttgctaggag
      361 gaatttacat ggaagagaaa aatttaacgg tcatcggaag ctatagtacg agggaagaag
      421 cattatcagt aattgaacgg ttgcgtaacg aagggtatga acgagatgat atcgttattt
      481 atactactga tgaagcagca tctagactag gatttgacgg attgtctggt atagatgttg
      541 aaacagatga aaatcggatg gatggtgaag aagaccgttc tttatgggaa aaaataaaag
      601 atactttttc ttttgatacc tatgattctg agacggctac accagaaaat gatcctttat
      661 atcaatatag aggagatatt tctgatggaa aatttgtcat cacagtaaaa ggctatcgtc
      721 agccagaaac aacagaagat acaatggata cacaagatac tgttagccca gcaagcacaa
      781 tggggactgg attagaaact ggcactggaa cagatccaat ggacgtaggt ggcacgacag
      841 gctttcaaaa tacaacggat ccattgaata tgacaccagg aactgatccg atggacgtac
      901 ctggcgagac tgatacaatg ggtacaagca agactgaaga catcgatcgc aaaccaaatc
      961 tagatgatga tacaattcaa ctaaaagaag aaaaattaga tgtaaataca tatgatgtga
     1021 caactggaga agtcgacatc cacaaacatg tggtcaacga tactgaaacg gtagaagtac
     1081 cggtcaaacg tgaagaaatc gtcatcgaac gcaaaccagt aacagatcaa tcttcacagg
     1141 gaacagacga aaaattggaa gatgacacga ttactatccc aatcaaagaa gagcaagtag
     1201 atgtaagcaa acatacagtt attcgtgaag aagttgggat tcataaagaa gaacatgaag
     1261 atgttgaaaa agtgacagaa gatgttagcc gagaagaatt ggatattgac acaagcggtg
     1321 atgtacacat tgaagaccgc aataaaaaat cgtaagcgta agctaaagag gttgtgacag
     1381 aagtggtcag cttaaagaaa tttcggctta tttccgaaga agctacttct ggaacaccgt
     1441 ttattcgcaa caaaaaaggg ctgtgacaag atttttgtca cagccctttt ttagggat
//
LOCUS       DACIRY010000223         1494 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.236, whole genome shotgun sequence.
ACCESSION   DACIRY010000223 DACIRY010000000
VERSION     DACIRY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.236"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            133..507
                     /locus_tag="IYE22_002746"
     CDS             133..507
                     /locus_tag="IYE22_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ9507922.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IYE22_002747"
     CDS             complement(621..1250)
                     /locus_tag="IYE22_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ9507923.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IYE22_002748"
     tRNA            complement(1384..1456)
                     /locus_tag="IYE22_002748"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
BASE COUNT          507 a          226 c          262 g          499 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaa
//
LOCUS       DACIRY010000224         1457 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.237, whole genome shotgun sequence.
ACCESSION   DACIRY010000224 DACIRY010000000
VERSION     DACIRY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.237"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            109..1041
                     /locus_tag="IYE22_002749"
     CDS             109..1041
                     /locus_tag="IYE22_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ9507924.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IYE22_002750"
     CDS             1113..>1457
                     /locus_tag="IYE22_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507925.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACIRY010000225         1430 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.238, whole genome shotgun sequence.
ACCESSION   DACIRY010000225 DACIRY010000000
VERSION     DACIRY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.238"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            98..286
                     /locus_tag="IYE22_002751"
     CDS             98..286
                     /locus_tag="IYE22_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ9507926.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            364..1377
                     /locus_tag="IYE22_002752"
     CDS             364..1377
                     /locus_tag="IYE22_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9507927.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
BASE COUNT          490 a          233 c          308 g          399 t
ORIGIN      
        1 tacgatagtg aacgtgctgg gagttttgtt cttttagaga aagtctctga agataaatta
       61 gttgtcttag attttgactg taacgaaaaa tggcgaaatg gaagagcacc agcaagtgat
      121 tgaccggatc tacgaagcga cacgatatct gcctttggat cgtttgtgct taagtcctca
      181 atgtggattt gcttcaacag aagaagaaaa tgttttgttt gaagaacaat ggaaaaaatt
      241 tcttttgtca aaagcattgc agaagaagtt tggggaaaat cattaaatca tctaggagag
      301 cttgatagga aataacacta tggtataatc agaatgctta atactaacta ctgagggagc
      361 cttatgaaaa agaaaattct taaatttatc ggatggttct tcggaatcat tcttgggatc
      421 gcgtttgcaa tctttcttgc cttccagctt tcccccagac ctggagcatt cttgatcaac
      481 catatgtttt caaatactgt tcaaatcact gataaaaaaa cgttcgatca agcagaaaaa
      541 aacgtggcga aaaaaacaga tttgacgtat gagtccaagt ataaagagaa tacttatgac
      601 atctactatc caaaaaacag caaggggcct gttcctgttc tattttgggt acatggtgga
      661 ggattcgtcg gcggagataa atcgggtgtc aaagaatttg cgacaaaatt agtttcagac
      721 gccaacatcg cagtggttgc gatgaattat gaattagcgc ctgattcaga ataccctaat
      781 caagttcttc aagtgaatga actgatcaaa gatttattag ctgaagaaaa agacgacaaa
      841 ttgctagaca tggaacaatt attttttggt ggagatagtg caggttcaca aatcgcattg
      901 caatatgcag ctatacaaac gaatgatgcg tatgctaaac aaatgaaaat caaacagcta
      961 cttccaaaag atagtctgaa aggaaccatt tcttattgcg gacctgttga tttgaagcta
     1021 acagcacagc agcattcgga tgatcgattt atgaagtttt tcgtcaaaac cgttgcgtgg
     1081 tcgttgattg gaacaaaaga ttggaaaacg agtgagcaat tgaaagaagc tagtgtggct
     1141 gaccatgttt ctaaggaatt tccgccaacg tatataactg acggaaatgc atactcattc
     1201 caggagcaag gtcaagcatt agaaaatcgg ttgacagaat taaaggtacc cgtgcaatcc
     1261 ttgttttata cagatacaaa aaaagagatt acccatgaat accaatttga ttacacgcta
     1321 aaagaatcgc aaaattgtta ccagcagaca ttggattttg tgaataagta taagtaagag
     1381 gttgtgaaaa agccgtttcg ttttgagtat gaagaaacga agcggctttt
//
LOCUS       DACIRY010000226         1427 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.239, whole genome shotgun sequence.
ACCESSION   DACIRY010000226 DACIRY010000000
VERSION     DACIRY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.239"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            73..1235
                     /locus_tag="IYE22_002753"
     CDS             join(73..349,349..1235)
                     /locus_tag="IYE22_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ9507928.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPRAGIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVRKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          479 a          274 c          308 g          366 t
ORIGIN      
        1 aatcggctac actagactag acagaaaaaa taaggtgtgt agaataataa aaaacacact
       61 ggaggatttt tcatgccaag acgtcaacga agaacctatt caaaagaatt caaacaacaa
      121 atcgtcgatc tctatctcgc tggtaagcct cgcgcaggaa ttattcgaga gtatgagctt
      181 acgccttctt ctttcgataa atggatgaag caagcacaat caacgggctc attcaaagaa
      241 agagacaact taacaccaga acaagcagaa ttgatcgcac taagaaagaa aaataagcaa
      301 ctcgaaatgg agaatgatat tttaaagcaa gcggcgctga tattcggacg aaaagacaag
      361 taattgatgc caacaagcat aaatattcca tatcagcgat gtgcaaaatt ctaaatattt
      421 ctcgtcagac ctactattat caagcgaaac cgatcgaaaa tgagtccgac ttagaagaga
      481 tcgtccagga agagtttatt cgaaaccgaa aggcttacgg tacccgaaaa ttgaagaagt
      541 gtttagcaaa gcgtgggctt caactcagtc ggcgccgaat cggtcgaatc atgaaacgcc
      601 gcggattgac atctacctat acgatcgctc attttaaagg gcaacgaaca gcttgtaatg
      661 aagcgaaaac agcgaatgta ttagatcgga cctttacaca agaacagcca ttggaagcca
      721 tcgttacgga tcttacttat gttcgcgtga ggaaaaagtg gcattatatc tgcttaatac
      781 ttgatttgtt taatcgagaa attattggtt attcctgtgg tgagaagaaa gatgcctcat
      841 tggtaaaaga agcctttgga cggataccgt attctttaac agacgtcaag ctttttcata
      901 cagaccgtgg aaaggaattt gataaccaaa ccattcatga gattctgaat ggttttggaa
      961 ttactcgttc attgagtagg aagggttgtc cgtatgataa tgccgttgtg gaatcaacct
     1021 ataaatctgt caaagtagaa ttcgtgcatc aataccaatt tgagacactg gcacagctac
     1081 gtctagaatt gtttgattat gtgcattggt ggaactatct acgcttacat ggcacgttgg
     1141 cgtatgaaac accgatccaa attcgacaac agagattggc gaagcgaatc cttgataatg
     1201 agcgcggatc tgatacctct ggagaggcag cgtaattgaa tgattgtgct tctgccggag
     1261 aaaatcagat ccgaggatgc tcattgtcaa gggcaatcgg agccataaca ccgcagcatt
     1321 cacaacacct tataattttt gtcaaaaaaa gtgttgccat tccactagtc taacttttgg
     1381 ggtgcacatc actttgaggg gcggtacata aaaatttatt atttaac
//
LOCUS       DACIRY010000227         1423 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.240, whole genome shotgun sequence.
ACCESSION   DACIRY010000227 DACIRY010000000
VERSION     DACIRY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.240"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            98..1390
                     /locus_tag="IYE22_002754"
     CDS             98..1390
                     /locus_tag="IYE22_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ9507929.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          257 g          411 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DACIRY010000228         1415 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.241, whole genome shotgun sequence.
ACCESSION   DACIRY010000228 DACIRY010000000
VERSION     DACIRY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.241"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            94..1389
                     /locus_tag="IYE22_002755"
     CDS             94..1389
                     /locus_tag="IYE22_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ9507930.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          279 c          265 g          421 t
ORIGIN      
        1 tcaccaacat cttttttgta taaaaaaata tagaaaagac accctgaaat cctttagaat
       61 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
      121 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      181 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      241 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      301 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      361 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      421 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      481 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      541 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      601 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      661 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      721 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      781 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      841 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      901 cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa
      961 ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt
     1021 aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta
     1081 cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca
     1141 ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact
     1201 ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca
     1261 aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc
     1321 tttcgcaact tttacaattt caaactacga attatgattc gtcatggcaa aacattttta
     1381 accaaataaa cagaacagaa agaggttttc ttctt
//
LOCUS       DACIRY010000229         1409 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.242, whole genome shotgun sequence.
ACCESSION   DACIRY010000229 DACIRY010000000
VERSION     DACIRY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.242"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(165..464)
                     /locus_tag="IYE22_002756"
     CDS             complement(165..464)
                     /locus_tag="IYE22_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ9507931.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            815..>1409
                     /locus_tag="IYE22_002757"
     CDS             815..>1409
                     /locus_tag="IYE22_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ9507932.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQAL"
BASE COUNT          474 a          226 c          200 g          509 t
ORIGIN      
        1 atactagctg tttttgtgtt aattaaagga taaaatacaa tataaagaaa catctgtttc
       61 tttatttaga ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg
      121 agattgacta tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa
      181 gtaatcggtg ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat
      241 atctccatat aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt
      301 aaactacgtt ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc
      361 tttgcgtcta tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca
      421 tataatttta gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa
      481 aaatatattt cttcccacta cattctataa acaaagtata cgctacatct agattttttt
      541 aaatcattac tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt
      601 aaaaacattc taaattaaat cagtaaaaat ttttattcta agatttttat tagaattacg
      661 cctaatttgt gtctctcagg gcaattctaa gaacgtttca agagatagcg acactatcgt
      721 tatcgaaaaa ggcgaaaacc tatctcattt tctgctaatg taagttggtt tagcttttgt
      781 taatatgact ttatgaaaat ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc
      841 aagcttgaat aaaggatcct tttttcatat ctatgaaaac gaagattttg aactgtataa
      901 tatcctttca caaaaagagg tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa
      961 atctattttc aagtatgcat tttttgcttc caatgatttt caaagaatgg agattaccga
     1021 ctgtatattc gaaaactgtg atttttcagg ctgtatattg acaaaaagca tgttgcatcg
     1081 cgtgaaattt gtcaattgta aattaacagg tactaaattg atggaaagtt atattgggaa
     1141 cacgttattc gagaattgta aaatggacta tgtaaattta tctggttcaa acataaaaga
     1201 aagtaccttt gagcactctg tcttatcaag cgcagatttt gtagactgtt ccctcactaa
     1261 aataatgttt tctactaatg atttagaaaa tgctagcttt tttgatactg atttaaaaaa
     1321 tatcgattta agtagcaact cctttgaaat gattgaagta cgctcggaac atctaaaaaa
     1381 ttgtacgctt aatcagttgc aagcacttg
//
LOCUS       DACIRY010000230         1381 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.243, whole genome shotgun sequence.
ACCESSION   DACIRY010000230 DACIRY010000000
VERSION     DACIRY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.243"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..355
                     /locus_tag="IYE22_002758"
     CDS             <1..355
                     /locus_tag="IYE22_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ9507933.1"
                     /translation="LQANQLEFEEKNIFAEPLTESEIKKILAATEGGVQDIISTRSKI
                     YEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDEIRSFVPREIRCV
                     ARNLIYLQNNNILNS"
     gene            369..725
                     /locus_tag="IYE22_002759"
     CDS             369..725
                     /locus_tag="IYE22_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ9507934.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          506 a          208 c          255 g          412 t
ORIGIN      
        1 gttgcaagcg aatcaacttg aatttgaaga gaagaatatt tttgccgaac cgttaacaga
       61 aagtgagatc aaaaagatct tggcagcgac tgaaggagga gttcaagata ttatttccac
      121 tcggtcgaaa atttacgaaa agctgggcat agattttaac gaattaactt taaagcaaat
      181 gatcgtctta ttcaaagaat atccttcatt attgagacga cctattttga ttgatgaaaa
      241 aagaatacta atcggattta atgaagatga gatccggtca tttgtccctc gtgaaattcg
      301 atgtgttgcc agaaatctga tctatttaca aaacaataac atcttaaatt catagggagg
      361 gagccttcgt ggaagtgaaa gtctatggat cagcgcactg tgtaacaagt ctgaaggcga
      421 aacaatggtt agaacaccat caattgcctt atcaatttgt tgacttagaa gaacgcgatc
      481 taagtacaga agaagcagaa gaattatgca gttttgaaga agtgcaagca gaacagctat
      541 ttgcgacttg gtctgaggct tttcacaaca ttgcgattga cctttcctgt gtaggaaaaa
      601 aacaactgat ttttttatgc acacaacata ccaatctcct cagacgtcct ttgattatta
      661 tcgatgaagt tctatttatt ggatacaatg agcagttgat ggaacagctt atcttaaata
      721 aatagacttt aactgtttta atcaagtcac tgagcatttc atacagttgt aacagtcgaa
      781 cacttagcat caagcgtgtt atgtgttctt tttttgactt tatttttcta ctatagagac
      841 atgctcaatt tatgtggtca aaattcaaat ctaggtgggg atatagagtt aaaaatatat
      901 tgacatgtgt aaatttacat gatatattca tttctgttgt tttaagtagc aaagcaataa
      961 aatagtattt taaaaaacat ataaaatagt tgttgacatg ttagaaatga catgataaga
     1021 taactgagtt gttaaaaaag aaccacttgt gaagcttgat tctatcgaaa aaaacttcaa
     1081 aaaagttctt gacaaataac aaatgataag ttatgataaa tgagttgctg taagatttcg
     1141 aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa ttacttgtct ctctgatatg
     1201 atataaaagt tgctacggca gccgaaaacg aatcggggaa cactcctgat gaagtagacc
     1261 tttgaaaact gaacaaagta agacaaacca aatgtgtagg gcgtcttgat tcaattcaag
     1321 acaacaaaca ttttttaaca agcaagcaat atgctagcaa acaaattgag cttaacaatc
     1381 g
//
LOCUS       DACIRY010000231         1336 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.244, whole genome shotgun sequence.
ACCESSION   DACIRY010000231 DACIRY010000000
VERSION     DACIRY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.244"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(146..1129)
                     /locus_tag="IYE22_002760"
     CDS             complement(146..1129)
                     /locus_tag="IYE22_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ9507935.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          285 c          254 g          420 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catttgcggt aataag
//
LOCUS       DACIRY010000232         1290 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.245, whole genome shotgun sequence.
ACCESSION   DACIRY010000232 DACIRY010000000
VERSION     DACIRY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.245"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(15..1154)
                     /locus_tag="IYE22_002761"
     CDS             complement(15..1154)
                     /locus_tag="IYE22_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ9507936.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          336 a          281 c          239 g          434 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt
//
LOCUS       DACIRY010000233         1273 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.246, whole genome shotgun sequence.
ACCESSION   DACIRY010000233 DACIRY010000000
VERSION     DACIRY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.246"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            32..529
                     /locus_tag="IYE22_002762"
     CDS             32..529
                     /locus_tag="IYE22_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9507937.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(566..1249)
                     /locus_tag="IYE22_002763"
     CDS             complement(566..1249)
                     /locus_tag="IYE22_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507938.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          443 a          216 c          224 g          390 t
ORIGIN      
        1 tatagtatac tatgtcaaaa ggaggagtta gatgaacaat caaatcacac tagcgtattt
       61 agattatgat gatctagatg ctttacaagt tgtaagtgtt gtcagttttt atgaatcgtt
      121 tatcgatggc gcagatccac ttgatatgca aggatattta cagacacaat tgacaactga
      181 aatattggca gtagaattgg cacagtcaac cagtaaattt attggtatta aggatcatca
      241 aatactcatt ggttatatga aagtcaatga tgaaaaagat gctatagaga ttcaacggct
      301 ctatctgctc aaagattatc aaaacaaagg gttagggcaa cggttactcg atgaagcgaa
      361 tcgttatgct aaaacgaaac aaaaacgtta tttacgtttg actgtttatg aaaaaaatca
      421 tgcaggtatt cgtttttatg aacgtcatgg atttaaaaaa atcgggatca aacattttcc
      481 tttaggaaaa caagatcgta tttgtccgat tttagaaaaa gaaatctaaa aaaacgtcct
      541 cacttttgat gctgaggacg tttttttaga ctggtaatgg aaccataatt cgctttcctt
      601 tttgataaat cgctaattcc gtcacaccat ttgcttttaa cagagcaaaa agatcatcga
      661 aatggaaacg gaaatgttca aggtaatgcc catctgatcc aacactaaac aaggtaccac
      721 cacgtgaaag atataaatcg atgacatatt catataacgc taaattgtca tacaagtaag
      781 cgctcttagc atttaattcg aatgccaatt tatttttgat tacttgatct agaatcggta
      841 aaaactgcgc ttcatattgt ttaaattcag caacggatag accaaatacc cgaaaaccat
      901 agtcaaaatg agcaaagaca tctgcttcta taacaactag tgcttctgaa agttgtgcaa
      961 aatactgagg aatcacaata gcaggatcca tatctttgac ttcgtcatct aaataatcaa
     1021 attgcccatt atgatgcaca ctgagtaatt ttaaatcgta atctttatcg gctaaatagt
     1081 cgatgatccg atccttttca gaagcgatgt aaccgatttt gatgcctttt aaaacacgat
     1141 tgccaaaacg cttatttaag ctatcgattg tttgactgta catttcataa tcggggatat
     1201 catcgacttt agtaacagga ttggctaagt caaaatgttc agttgtcacg acgtaaccat
     1261 cgtagtttgt taa
//
LOCUS       DACIRY010000234         1235 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.247, whole genome shotgun sequence.
ACCESSION   DACIRY010000234 DACIRY010000000
VERSION     DACIRY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.247"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            414..947
                     /locus_tag="IYE22_002764"
     CDS             414..947
                     /locus_tag="IYE22_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ9507939.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          396 a          228 c          232 g          379 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcg
//
LOCUS       DACIRY010000235         1192 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.248, whole genome shotgun sequence.
ACCESSION   DACIRY010000235 DACIRY010000000
VERSION     DACIRY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.248"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1076
                     /locus_tag="IYE22_002765"
     CDS             <1..1076
                     /locus_tag="IYE22_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ9507940.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DACIRY010000236         1178 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.249, whole genome shotgun sequence.
ACCESSION   DACIRY010000236 DACIRY010000000
VERSION     DACIRY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.249"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            82..>1178
                     /locus_tag="IYE22_002766"
     CDS             82..>1178
                     /locus_tag="IYE22_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ9507941.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDD"
BASE COUNT          391 a          227 c          228 g          332 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacag
//
LOCUS       DACIRY010000237         1136 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.250, whole genome shotgun sequence.
ACCESSION   DACIRY010000237 DACIRY010000000
VERSION     DACIRY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.250"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1061)
                     /locus_tag="IYE22_002767"
     CDS             complement(<1..1061)
                     /locus_tag="IYE22_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9507942.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          301 a          254 c          182 g          399 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaac
//
LOCUS       DACIRY010000238         1127 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.251, whole genome shotgun sequence.
ACCESSION   DACIRY010000238 DACIRY010000000
VERSION     DACIRY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.251"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..222
                     /locus_tag="IYE22_002768"
     CDS             <1..222
                     /locus_tag="IYE22_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9507943.1"
                     /translation="FEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQT
                     SANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            388..585
                     /locus_tag="IYE22_002769"
     CDS             388..585
                     /locus_tag="IYE22_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ9507944.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            587..898
                     /locus_tag="IYE22_002770"
     CDS             587..898
                     /locus_tag="IYE22_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ9507945.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            1002..>1127
                     /locus_tag="IYE22_002771"
     CDS             1002..>1127
                     /locus_tag="IYE22_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ9507946.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          438 a          135 c          203 g          351 t
ORIGIN      
        1 ttcgaagacg cctttcaata taccgtacaa ggaaattccc acaatcgact aaagagtacc
       61 aatctaattg aacgactgaa tcaagaagta cgcagaagag aaaagattat tcgcatcttc
      121 cccaatcaaa catcagccaa tcgcttaatt ggagccgttc ttatggacct gcatgatgaa
      181 tggatttatt cttcaagaaa atacatcaat tttgataagt agaaatggta aaaacattgt
      241 atagcatttt acacaggagt ctggacttga ctgattaaat tcttattcag gaattatttt
      301 gctacgtttc aagaaggaat tgttttttga aatttaatga atgttagaat aatagtctaa
      361 aaacattaat ttaggggtga ttagattatg aaacatttaa aaattttgtc tattaaagag
      421 acacaactta tctatggggg taccactcat agtggaaaat attatggaaa tggagtgtat
      481 tgcactaaaa ataaatgtac ggtcgattgg gccaaggcaa ctacttgtat tgcaggaatg
      541 tctataggtg gttttttagg tggagcaatt ccagggaagt gctaaaatga aaaaaaatgc
      601 taagcaaatt gttcatgaat tatataatga tatatctata agtaaagatc ctaaatattc
      661 tgatattctt gaggttttac aaaaggtata tttaaaatta gaaaaacaaa aatatgaatt
      721 agatcccagt cctttaataa atagattggt gaattatcta tattttactg cttatactaa
      781 taaaataaga ttcactgaat atcaagagga attaataaga aatttgagtg aaattggaag
      841 aactgctgga ataaatggtt tatatcgagc agattatgga gataaatctc aattttaatg
      901 atacttataa tattaaatat gtctcaaaat tttttgatta gaaaaatagg tatattgaat
      961 cagtgacata aaagtattta tagtttgtag ggggaatcgt aatggaagaa aaaaatagac
     1021 taaatgctaa acaatgttca gatcaagagc ttaaaaaaat taaaggaggt gctggaacaa
     1081 aacctcaagg aaaaccggca tcaaatttag tggaatgtgt tttttca
//
LOCUS       DACIRY010000239         1091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.252, whole genome shotgun sequence.
ACCESSION   DACIRY010000239 DACIRY010000000
VERSION     DACIRY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.252"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            6..980
                     /locus_tag="IYE22_002772"
     CDS             6..980
                     /locus_tag="IYE22_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ9507947.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACIRY010000240         1083 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.253, whole genome shotgun sequence.
ACCESSION   DACIRY010000240 DACIRY010000000
VERSION     DACIRY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.253"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            13..636
                     /locus_tag="IYE22_002773"
     CDS             13..636
                     /locus_tag="IYE22_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507948.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          379 a          163 c          221 g          320 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaa
//
LOCUS       DACIRY010000241         1073 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.254, whole genome shotgun sequence.
ACCESSION   DACIRY010000241 DACIRY010000000
VERSION     DACIRY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.254"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            2..>1073
                     /locus_tag="IYE22_002774"
     rRNA            2..>1073
                     /locus_tag="IYE22_002774"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACIRY010000242          990 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.255, whole genome shotgun sequence.
ACCESSION   DACIRY010000242 DACIRY010000000
VERSION     DACIRY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.255"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            62..>990
                     /locus_tag="IYE22_002775"
     CDS             62..>990
                     /locus_tag="IYE22_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ9507949.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKA"
BASE COUNT          367 a          182 c          177 g          264 t
ORIGIN      
        1 taacagtcct tttacggtac aatgttttta acgacaaaaa catacccagg aggactttta
       61 catgacccaa gtacatttta cactgaaaag cgaagagatt caaagcatta ttgaatattc
      121 tgtaaaggat gacgtttcta aaaatatttt aacaacggta tttaatcaac taatggaaaa
      181 tcaacgaaca gaatatattc aagcaaaaga atatgaacga acagaaaacc gacaaagtca
      241 acgaaatggc tattatgagc gcagctttac gacacgtgta ggcacgctag aattaaaagt
      301 acccagaaca cgtgatggcc atttttcacc cacagtgttt gaacgttatc aacgaaacga
      361 aaaagccctc atggcttcaa tgttggaaat gtatgtatca ggcgtttcaa ctcgtaaagt
      421 atcaaaaatt gtggaagaac tttgtggtaa atccgtctct aagtccttcg tttctagctt
      481 aacagaacag ctagaaccta tggttaacga gtggcagaat cgtttattat cagaaaaaaa
      541 ttatccttac ttaatgaccg atgtactcta tataaaagta cgagaagaaa atcgagtact
      601 ctcaaaaagc tgtcatatag cgattggaat aaccaaagat ggcgaccgtg aaattatcgg
      661 cttcatgatt caaagtggcg aaagcgaaga gacctggaca acattttttg aatacctaaa
      721 agaacgcggt ttacaaggta cggaactcgt tatttctgat gcgcacaaag gattagtctc
      781 tgccattaga aaatccttca ccaacgtaag ttggcaaaga tgccaagttc acttcctaag
      841 aaatatcttt accaccattc ctaaaaaaaa ttcaaaatct ttcagagaag ctgttaaagg
      901 aatttttaag ttcacagata ttaacttagc gcgtgaggct aaaaatcgat tgattcatga
      961 ttatatcgat caaccaaaat attcaaaagc
//
LOCUS       DACIRY010000243          908 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.256, whole genome shotgun sequence.
ACCESSION   DACIRY010000243 DACIRY010000000
VERSION     DACIRY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.256"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            82..>908
                     /locus_tag="IYE22_002776"
     CDS             82..>908
                     /locus_tag="IYE22_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ9507950.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQK"
BASE COUNT          303 a          163 c          177 g          265 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaag
//
LOCUS       DACIRY010000244          807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.257, whole genome shotgun sequence.
ACCESSION   DACIRY010000244 DACIRY010000000
VERSION     DACIRY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.257"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IYE22_002777"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IYE22_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ9507951.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          297 a          126 c          152 g          232 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcagg
//
LOCUS       DACIRY010000245          791 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.258, whole genome shotgun sequence.
ACCESSION   DACIRY010000245 DACIRY010000000
VERSION     DACIRY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.258"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..782
                     /locus_tag="IYE22_002778"
     CDS             <1..782
                     /locus_tag="IYE22_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9507952.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DACIRY010000246          791 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.259, whole genome shotgun sequence.
ACCESSION   DACIRY010000246 DACIRY010000000
VERSION     DACIRY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.259"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            24..716
                     /locus_tag="IYE22_002779"
     CDS             24..716
                     /locus_tag="IYE22_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ9507953.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DACIRY010000247          767 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.260, whole genome shotgun sequence.
ACCESSION   DACIRY010000247 DACIRY010000000
VERSION     DACIRY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.260"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..>767)
                     /locus_tag="IYE22_002780"
     CDS             complement(<1..>767)
                     /locus_tag="IYE22_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9507954.1"
                     /translation="EQINEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATG
                     VGKSFLATKIFEYAIHEQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEK
                     DTDGLLATANNGYLFLDEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFA
                     TTEKGENYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSRE
                     ALSLMLSHPFSGNIGKLKNIIKISCADVYSRTQEEPLT"
BASE COUNT          230 a          162 c          134 g          241 t
ORIGIN      
        1 gttaatggtt cttcttgtgt tcgactatac acatccgcac aactgatttt aatgatattc
       61 ttcagcttac caatattccc actaaaggga tgagataaca ttaaagacaa ggcttctctt
      121 gaaacaataa tatctttttg taaaacatcc gcttcatttt gaaaaaataa ttgaatcagc
      181 tctaatcgtt ctcgaatggg gcgatcttca aaagttggca acataacact aatttgaata
      241 cgccgatcaa aggtgtctaa taaataattt tcaccttttt ccgtcgtggc gaaaataaaa
      301 cgaaccttag cagaatgcca atttacattt tcaccgactg gacgatagtt tccagtatca
      361 ataaatagaa agagcttttc ttgattttct ttcgataggc gatggacctc atcgagaaaa
      421 agatagccgt tgttggccgt tgccaataaa ccatcggtat ccttttcagc ccccgtaaag
      481 gcccccttct tgtatccaaa cagtgttgcc gaaagcagtt cgggattgtc cgcataatcg
      541 gcacagttta agatagcaaa tggtgcatct ttttcaataa tttgctcatg gatagcgtat
      601 tcaaaaattt ttgtggctaa aaaacttttt ccaacacccg tggctccatt tattaataca
      661 ctgagtccgt taggcggata tttgacagcg gctatacatt tttgaatgac ctctctcaga
      721 cttccgtcat gaccaactac agaatgaaag gcttcgttta tttgctc
//
LOCUS       DACIRY010000248          751 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.261, whole genome shotgun sequence.
ACCESSION   DACIRY010000248 DACIRY010000000
VERSION     DACIRY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.261"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(325..>751)
                     /locus_tag="IYE22_002781"
     CDS             complement(325..>751)
                     /locus_tag="IYE22_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ9507955.1"
                     /translation="VLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLAVK
                     AQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERYVG
                     TEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
BASE COUNT          228 a          156 c          113 g          254 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac t
//
LOCUS       DACIRY010000249          696 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.262, whole genome shotgun sequence.
ACCESSION   DACIRY010000249 DACIRY010000000
VERSION     DACIRY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.262"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(53..628)
                     /locus_tag="IYE22_002782"
     CDS             complement(53..628)
                     /locus_tag="IYE22_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9507956.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          125 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaacg
//
LOCUS       DACIRY010000250          692 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.263, whole genome shotgun sequence.
ACCESSION   DACIRY010000250 DACIRY010000000
VERSION     DACIRY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.263"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..605)
                     /locus_tag="IYE22_002783"
     CDS             complement(<1..605)
                     /locus_tag="IYE22_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507957.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT          224 a          134 c           99 g          235 t
ORIGIN      
        1 atcaattcgt catatcttcc aaaatcaaca ttgtactcat aaccaagaag tgtcggaatc
       61 aggtcaattt caatttcttt ttcaattgaa cgtttaataa gtttcgtgga tacatacccg
      121 acaaaaaaga gaataatacc cacttcaaac aaatacatgt gtccatttac aaagtgaata
      181 aacgtcaata aaaaatcctt cactcctttt gttgttcttc cagattgttc aatttgtttt
      241 attaaagtcg ctagaattgc atttacaaca attaaatcga ataggctttt gaaaagtttt
      301 cctgctgtta atttttgcct ggtaaattgc atgactaatt gagtaaaaat agacaatagc
      361 atgacaaaaa tcaacgtcat tagcactgta aaaatgtctg atgctgcaat cctattggga
      421 tcatttatca agtaataagc cgttccaaat actccaatcg agtaaacggt taaatcaaga
      481 ataagtcgtg ccccgtaact acaaagtttt cctaatggcc ataattgcaa tacccaaaat
      541 ttctttcgtt tgctatttct tttcagagga agccaggtgt gttcaatcca gtcgtccgat
      601 ttcatttcga tttcttccct aaattctttc ctttaacgtt ttcctttgct actttagcag
      661 cgataggttg ctcaatgaac caatttttta ag
//
LOCUS       DACIRY010000251          659 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.264, whole genome shotgun sequence.
ACCESSION   DACIRY010000251 DACIRY010000000
VERSION     DACIRY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.264"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..>659
                     /locus_tag="IYE22_002784"
     CDS             <1..>659
                     /locus_tag="IYE22_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ9507958.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPR
                     KNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVL
                     L"
BASE COUNT          225 a          123 c          115 g          196 t
ORIGIN      
        1 gccaaagagc cacgtataga gcggtttgtt ccgtcttatt tcctaatggt gactttccta
       61 gtaggagtcg attcactcgc ttaagttcaa acttagctta cactatcaag attattcgat
      121 actttttcat caagaagctc accaaaggtg aactagtcgg aattatagat agttttccta
      181 gtccattatg taaaccagtt agaaatagac aagcaaaact attgaatcaa atagccaaag
      241 ttggctataa ctcaacaaaa aaatcttatt tttatggtct taaaattcat atgatcgtta
      301 ctaagactgg ctttccgatt acttactcaa tcacgaatcc aggtgtccat gacgtgaaag
      361 tgttagagac tttatcggaa gaagcgaatc ttcccaatat cctaggggac aaaggatata
      421 ttagccataa aatccatgaa aagcttgccc ttaagggcat tactatatcc gttccgccac
      481 gtaaaaacat ggataaatca gaaaaactag accacagctt acttggaaaa caaagaaaaa
      541 cagttgagac tgttttttct tccttagaaa aattaggttg tcaaaatttc aactcacgtt
      601 ctgttaaagg gctagagagt agatttgaaa gcatattact ggcttacagt gttctatta
//
LOCUS       DACIRY010000252          629 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.265, whole genome shotgun sequence.
ACCESSION   DACIRY010000252 DACIRY010000000
VERSION     DACIRY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.265"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..>629
                     /locus_tag="IYE22_002785"
     CDS             <1..>629
                     /locus_tag="IYE22_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ9507959.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYS"
BASE COUNT          214 a          121 c          105 g          189 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattct
//
LOCUS       DACIRY010000253          619 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.266, whole genome shotgun sequence.
ACCESSION   DACIRY010000253 DACIRY010000000
VERSION     DACIRY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.266"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(26..379)
                     /gene="tnpB"
                     /locus_tag="IYE22_002786"
     CDS             complement(26..379)
                     /gene="tnpB"
                     /locus_tag="IYE22_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ9507960.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(369..563)
                     /locus_tag="IYE22_002787"
     CDS             complement(369..563)
                     /locus_tag="IYE22_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507961.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          179 a          133 c          103 g          204 t
ORIGIN      
        1 acattttttg tgttaacccc ccagcttaaa aaactccttt ttttcctggc aaaatagctc
       61 gaggttgatc gatacttaat ccttctaaca accatcgaac ctgttggttc gttaaggttc
      121 gaacttcgtc tttcgtccga ggccatttta acttgccgtt ttctatccgc ttataacaga
      181 gaagaaaacc atcaccgtcc caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa
      241 ataaaaaaac ggcatcttcg taaagctcga gttgatactc ttgttgaata agactggcaa
      301 gaccatcaat tccttttcgc aaatctgttt ttccgcacac aatataaata tgtttggttt
      361 gaagtaaatc ataaagcatt ggacaacagt tctttcaaaa tagcttgaag aatataattg
      421 ttaataccgt tatagatcaa aacttcagaa ccattctctc ttttgatgcg catcgctaac
      481 ttggcagccg cgggtttacc tttttctggt gcactctttt cgtttcctaa ttggacagga
      541 acaatctcat ttaatttata catagaaaac ctccagactt cttgatagct caagtatatc
      601 ttgaggtgca tttgttagt
//
LOCUS       DACIRY010000254          617 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.267, whole genome shotgun sequence.
ACCESSION   DACIRY010000254 DACIRY010000000
VERSION     DACIRY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.267"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..614)
                     /locus_tag="IYE22_002788"
     CDS             complement(<1..614)
                     /locus_tag="IYE22_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ9507962.1"
                     /translation="MPFQTSAKITIENLHPKDMSSFFYTINYALVDELEENSLYFHAN
                     WHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLERYWWGEGEFKFYIDGDSEYPTV
                     TSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYPYQSNRDATRDRFETGKLNAVH
                     AFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGNDDVRLFER"
BASE COUNT          177 a          140 c          122 g          178 t
ORIGIN      
        1 tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt gtaaagtgac cgataaatct
       61 tcggtaaaac agatggggtc ttgaagatgc caacgataaa gaccatgtcg tggcattgcg
      121 tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt caaaacgatc tcttgtggca
      181 tctctatttg attggtaagg ataccctaaa aaggctgttg aatagttttt cgcatgtgga
      241 cgtccgaact catcgtactc atgaaaggcc cacgccccgc caaaataatc ttctgaacct
      301 gttgaggtta cggtcggata ctcactatcc ccatcaatgt agaatttaaa ctcgccttcg
      361 ccccaccaat aacgttctag gcaggtaagc gcaaggtaag tacccacgta atagccttga
      421 ccctttaatc gatcaattaa cttataatcc tcctgtaatt tggtaatgcg ttcccgatgc
      481 caatttgcgt gaaaatatag gctattttct tccaattcgt ccaccaaagc gtaatttatc
      541 gtataaaaaa atgaggacat atccttcgga tgtaaattct cgatcgtaat tttagcggat
      601 gtttggaaag gcatttc
//
LOCUS       DACIRY010000255          591 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.268, whole genome shotgun sequence.
ACCESSION   DACIRY010000255 DACIRY010000000
VERSION     DACIRY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.268"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
BASE COUNT          231 a           92 c           91 g          177 t
ORIGIN      
        1 ccacacctta taaaaatctt tgacaaattt ttctgatgga tcgtatttaa accaaagcag
       61 atgttcctct agctcgattg tcatgttttt cacctcaacg aaagtatacg aactaatgtt
      121 tgttttgtaa aggataaaaa tagggtagca accggtaccc aaactagtac ccatttatca
      181 aaaacgacga aatttatgta aatagaaaaa taagaataaa cattgatgta tcaacattta
      241 tatatcttta gaacaaatga aaagggtggt tctcataaga aaaacatgtt cgcacttcta
      301 ggcaaaccag gatttgaaga ccttgctaaa gaattgaacg aacgcctgta aaataaggat
      361 ttgtcgctag atttaaaaaa tcagtgatag aaacatgttt gctttaaaat gaaactagtg
      421 atagttttag tgatagtgaa cttaaaaaaa gcactcaatt tgagtgcttt tttattgtta
      481 ttaaaaatta aaaaactaaa atacataccc gaatttcata cccgaaatat aaaaaatgtc
      541 atcacgtaca tctttttcga aactaccgaa gtagccaaag atgtactgat g
//
LOCUS       DACIRY010000256          572 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.269, whole genome shotgun sequence.
ACCESSION   DACIRY010000256 DACIRY010000000
VERSION     DACIRY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.269"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            141..398
                     /locus_tag="IYE22_002789"
     CDS             141..398
                     /locus_tag="IYE22_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507963.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          240 a           70 c           80 g          182 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tc
//
LOCUS       DACIRY010000257          529 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.270, whole genome shotgun sequence.
ACCESSION   DACIRY010000257 DACIRY010000000
VERSION     DACIRY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.270"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..503)
                     /locus_tag="IYE22_002790"
     CDS             complement(<1..503)
                     /locus_tag="IYE22_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ9507964.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFS"
BASE COUNT          152 a          133 c           91 g          153 t
ORIGIN      
        1 gaaaaattgt cctttggatg tcgcttgatc aacatatgcc ccatttccgg atgactgaac
       61 ccttccacga aagtcccatt gaacaaggca ccacctccga tacctgttcc cacagtgaag
      121 tatgcaacac ctgctaaccc tttgccagaa ccagcaacat attcaccata gcaagccgca
      181 ttgacatctg ttgtccattc aatcggaata ttgaaatatt gctttagcgt tcctacaaaa
      241 ttgaaatttt gccaagcaag ttttggtgtt gatgtgatat gaccgaaact cgttgatttg
      301 cattgaatat caattggacc aaagcagccg accccgatgc ctgccaattg ttccttaaaa
      361 ggtttaaaaa aatcaatgac ctgcttcatt gtttcaactg gagttgttgt gggaaaactg
      421 actctttcca aaatcgttaa ttcctcagta ccgactgcac agacaaattt agtccctcca
      481 gcttcaattg ccccatatat catcataggt tcctcccttc gaatatgtg
//
LOCUS       DACIRY010000258          521 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.271, whole genome shotgun sequence.
ACCESSION   DACIRY010000258 DACIRY010000000
VERSION     DACIRY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.271"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..434)
                     /locus_tag="IYE22_002791"
     CDS             complement(<1..434)
                     /locus_tag="IYE22_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9507965.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..>521)
                     /locus_tag="IYE22_002792"
     CDS             complement(451..>521)
                     /locus_tag="IYE22_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ9507966.1"
                     /translation="TTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          149 a           88 c           82 g          202 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc a
//
LOCUS       DACIRY010000259          519 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.272, whole genome shotgun sequence.
ACCESSION   DACIRY010000259 DACIRY010000000
VERSION     DACIRY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.272"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..493)
                     /locus_tag="IYE22_002793"
     CDS             complement(<1..493)
                     /locus_tag="IYE22_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ9507967.1"
                     /translation="MIYGAIEAGGTKFICAVSTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQRKSKKYGHITSTPKLAWQNFNFVGTLKQHFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPK"
BASE COUNT          148 a          134 c           84 g          153 t
ORIGIN      
        1 cctttggatg tcgcttgatc aacatatgcc ccatttccgg atgactgaac ccttccacga
       61 aagtcccatt gaacaaggca ccacctccga tacctgttcc cacagtgaaa tatgcaacac
      121 ctgctaaccc tttgccagaa ccagcaacat attcaccata gcaagccgca ttaacatctg
      181 ttgtccattc aatcggaata ttgaaatgtt gctttagcgt tcctacaaaa ttgaaattct
      241 gccaagcaag tttcggtgtt gatgtgatat gaccgtattt ctttgattta cgttgaatat
      301 caattggacc aaaacagccg accccgattc ctgccaattg ttccttaaaa ggtttaaaaa
      361 aatcaatgac ctgcttcatt gtttcaactg gagtcgttgt gggaaaactg actctttcca
      421 aaatcgtcaa ttcctcagta ctgactgcac aaataaattt agtccctcca gcttcaattg
      481 ccccatatat catcataggc ttctcccttc gaatatgtg
//
LOCUS       DACIRY010000260          517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.273, whole genome shotgun sequence.
ACCESSION   DACIRY010000260 DACIRY010000000
VERSION     DACIRY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.273"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..>517
                     /locus_tag="IYE22_002794"
     CDS             <1..>517
                     /locus_tag="IYE22_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ9507968.1"
                     /translation="CGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTI
                     YRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGD
                     TIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDC
                     GKEFSNWKQISN"
BASE COUNT          170 a           88 c          108 g          151 t
ORIGIN      
        1 tgtggtagac gtccgcttgt tttacccgag gaacaatcag aatatattca aagaaaggtt
       61 gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat tagttgttct
      121 gctcgtacca tttatcgtat gttcaaaaag gggctatttg attcttctga cttaccgatg
      181 aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac tttccgccgc
      241 tctattcatg aacgtgaaaa ggattatagc caattctcaa atgagtttgg tcaccttgaa
      301 ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt tgaacgatta
      361 tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag cgattgatat tgaaaaaaaa
      421 ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca aatccatcac ttttgattgt
      481 ggaaaggaat tttcaaattg gaaacagatc agtaatg
//
LOCUS       DACIRY010000261          504 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.274, whole genome shotgun sequence.
ACCESSION   DACIRY010000261 DACIRY010000000
VERSION     DACIRY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.274"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..474
                     /locus_tag="IYE22_002795"
     CDS             <1..474
                     /locus_tag="IYE22_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ9507969.1"
                     /translation="FYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAA
                     YAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHR
                     PVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          173 a           91 c          101 g          139 t
ORIGIN      
        1 ttttattcat ggaaaatgga tgaaacgtac atcaaaatta aaggaaaatg gcattatttg
       61 tatcgagcca tcgatgcaga tggtttaacc ttggatattt ggttacgtaa aaaacgggac
      121 acacaagcag cctatgcttt tcttaagcgg ttagtgaagc agtttgatga accgaaggtt
      181 gtagtcacag ataaagcccc ctctattaca agtgccttta agaaactaaa agaatacggc
      241 ttttatcaag ggacagaaca tcgtaccatt aaatacctga ataatttgat tgaacaagac
      301 catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc ctctaccacg
      361 attaaaggca tggaagccat tcgaggatta tataagaaaa cccgaaaaga aggcactctc
      421 ttcgggtttt cggtctgtac tgaaatcaag gtattattgg gaatcccagc ttaaatcata
      481 gataccgtaa gggattttat tctt
//
LOCUS       DACIRY010000262          490 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.275, whole genome shotgun sequence.
ACCESSION   DACIRY010000262 DACIRY010000000
VERSION     DACIRY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.275"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..415)
                     /locus_tag="IYE22_002796"
     CDS             complement(<1..415)
                     /locus_tag="IYE22_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507970.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          187 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat
       61 ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag
      121 ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat
      181 tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg
      241 cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt
      301 ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa
      361 atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg
      421 tcaatggtat taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct
      481 caaaaacata
//
LOCUS       DACIRY010000263          483 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.denovo.276, whole genome shotgun sequence.
ACCESSION   DACIRY010000263 DACIRY010000000
VERSION     DACIRY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.denovo.276"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..338)
                     /locus_tag="IYE22_002797"
     CDS             complement(<1..338)
                     /locus_tag="IYE22_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ9507971.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEE"
BASE COUNT          165 a           68 c           52 g          198 t
ORIGIN      
        1 ttttcttcat ctgttttatt atatggggat gttttcgtta tatataacaa cttttctttt
       61 tcaattttca ttaataactc tgtcgaagtc tgtactttat cttgataatt aaatgcagac
      121 ataccacttt gtataattgt aattattgct gaagcaattg aaacaatcaa tagtaaagac
      181 ctgtgttcgg aagcctgatc aattaatatt gggatactag ctgataagac tatttttata
      241 atatttccga taccaattac tctattataa aatcgaattt ctttttttaa tctatcgatt
      301 gtagaatcaa tagattcgat aaacgctttt tgatccatca ttttctctcc aatcatttta
      361 ttaaaatcat taacaaaaat attataatag gatttaagta agttctaact aatcacatat
      421 aaaaataaaa aaacatttgt attttatcaa agttttatta tacttgtcaa tttgtgtaca
      481 tcc
//
LOCUS       DACIRY010000264        19933 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.045, whole genome shotgun sequence.
ACCESSION   DACIRY010000264 DACIRY010000000
VERSION     DACIRY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.045"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..375
                     /locus_tag="IYE22_002798"
     CDS             <1..375
                     /locus_tag="IYE22_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAQ9507972.1"
                     /translation="VEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKN
                     QSISFDWEYMFAKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQ
                     ATKDVETGHMLGKVVVSGPFNG"
     gene            complement(464..787)
                     /locus_tag="IYE22_002799"
     CDS             complement(464..787)
                     /locus_tag="IYE22_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ9507973.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(774..1481)
                     /locus_tag="IYE22_002800"
     CDS             complement(774..1481)
                     /locus_tag="IYE22_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ9507974.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            1567..1908
                     /locus_tag="IYE22_002801"
     CDS             1567..1908
                     /locus_tag="IYE22_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ9507975.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(2079..2927)
                     /locus_tag="IYE22_002802"
     CDS             complement(2079..2927)
                     /locus_tag="IYE22_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507976.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            3525..4388
                     /locus_tag="IYE22_002803"
     CDS             3525..4388
                     /locus_tag="IYE22_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9507977.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            4488..4970
                     /locus_tag="IYE22_002804"
     CDS             4488..4970
                     /locus_tag="IYE22_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9507978.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5233..5700)
                     /locus_tag="IYE22_002805"
     CDS             complement(5233..5700)
                     /locus_tag="IYE22_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ9507979.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            5823..6674
                     /locus_tag="IYE22_002806"
     CDS             5823..6674
                     /locus_tag="IYE22_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9507980.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(6925..8883)
                     /locus_tag="IYE22_002807"
     CDS             complement(6925..8883)
                     /locus_tag="IYE22_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9507981.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(8880..9752)
                     /locus_tag="IYE22_002808"
     CDS             complement(8880..9752)
                     /locus_tag="IYE22_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9507982.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(10094..10966)
                     /locus_tag="IYE22_002809"
     CDS             complement(10094..10966)
                     /locus_tag="IYE22_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9507983.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            11094..11663
                     /locus_tag="IYE22_002810"
     CDS             11094..11663
                     /locus_tag="IYE22_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ9507984.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            11730..12467
                     /locus_tag="IYE22_002811"
     CDS             11730..12467
                     /locus_tag="IYE22_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9507985.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            12698..13966
                     /locus_tag="IYE22_002812"
     CDS             12698..13966
                     /locus_tag="IYE22_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ9507986.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            14453..14917
                     /locus_tag="IYE22_002813"
     CDS             14453..14917
                     /locus_tag="IYE22_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9507987.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            14901..15530
                     /locus_tag="IYE22_002814"
     CDS             14901..15530
                     /locus_tag="IYE22_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ9507988.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            15884..17362
                     /gene="msr"
                     /locus_tag="IYE22_002815"
     CDS             15884..17362
                     /gene="msr"
                     /locus_tag="IYE22_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ9507989.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            17409..17855
                     /locus_tag="IYE22_002816"
     CDS             17409..17855
                     /locus_tag="IYE22_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ9507990.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18054..18569)
                     /locus_tag="IYE22_002817"
     CDS             complement(18054..18569)
                     /locus_tag="IYE22_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9507991.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            18686..19887
                     /locus_tag="IYE22_002818"
                     /pseudo
     CDS             18686..19887
                     /locus_tag="IYE22_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6318 a         3549 c         4107 g         5959 t
ORIGIN      
        1 gtagagtatg tcgctgtatt attcgatatt acccgctact ttgatcaaat caagcacctt
       61 gtccgaccat ttggacatgt gggaacgatc gtagggatca aagagccgtt agatataggt
      121 gtttggaaaa accaatctat cagcttcgac tgggagtata tgttcgctaa aacagactat
      181 aactatgaaa tcgaaacgca aggagaagct ttagcgcaca ttgctgcttt agcaaatgaa
      241 gggattatcc gcagtacatt gagcaaggtc tattcagatg ggatgaatgc gaagaacttg
      301 aaacaagcaa ccaaagacgt agaaacagga catatgttag gaaaagtcgt tgttagtggt
      361 ccgttcaacg gataagcaag aatacgccga tttttttctt atttgacttt gataaaaaca
      421 agagcgcatg acagtaattt gccataagct cttgtttttt tatttataat aagcgaatca
      481 acgaaaggga aagaattcct gccaagacga tcaccaataa gctttttgaa agaatagcag
      541 ctaaaagtgt aggaactgaa gccaaagcat tttcgagatt gatctgcggc agatgtccga
      601 tattttgggt aaatagactg ctgaaccata aagcagacat aatgacgatc gggacaaaac
      661 ttaaatactc aagcaacggt tgcggtaaat cgaatttttt cagtaagaca aatggtagga
      721 ccctagaaag ccaagttgct aaagtacaac ctaaaatagt cagcaatgta aactcaaaag
      781 aaggcatgtt tgatccacac ccccaatccg cacccaatca gtgttacaac tacaatcact
      841 aagttgcttg ggataaaaat cagtccgaca tataccagaa tcaaagtgag accaatcagg
      901 atcaactgta atcgtttaga ggtgtttcta tcagaaatga cttgaagata aagtaacccg
      961 ataaacatag cgacgatggc gaaatccaaa ccaaactttt tcggatcagt aataaaattc
     1021 cctaaaagtg cgccaacaag agaagaaccc acccacgtca aataagaaat ccaattagct
     1081 gtattcatcc aacgaaagtt taacttttgt ccagtatagt tcaatttatt cattcccagt
     1141 gcgaaacttt catctgtcag caatgtgcca atcagcagat tttgaagcag ccgatttttc
     1201 ttgaaataag gagcaatcgt catactcatc agaatcattc gggaattgac tagaaaggtc
     1261 gagaaaacga tggaaagcaa tgggctgtga ctagcaagca tgctgactgt gataaattgg
     1321 gcagaaccag catagaccag cagggacatc aacgtgacga ctagtggatg aaatccggca
     1381 gctttcccaa cgataccaaa tgcaagtcca ataccgatat aaccgaaaac ggtaggtaac
     1441 gtgtctttga tcgcagtttt tatatctaac ttttcatcca ataacaaaac tcctttagca
     1501 cgacataaat tttcataatt gtcgttaaac ttgtgaatat tctaaatttt taatgagcat
     1561 ttgtcaatgg tgggattgaa acgtaaagaa ctagcgggtc gcagatttac acgtattgtt
     1621 tcggtgggag gaggcgcgaa aaatcttgca tggctacaaa tccaagctga tattttcgat
     1681 gcaacggtct attgtttaag tgtggaggaa ggacctgcat taggcgcaac aatgattgct
     1741 gcactagggt tagggtggtt cgacacacct gaaagttgtt gtcagaattt tatccaatat
     1801 acagatgcag cggaacctct cttacaaaat gtagaaaagt atcataaaat ctatcaacaa
     1861 tgccggaaaa tctatccagc aacgaaggaa atttgtcatg acttataaaa caaaacgcgt
     1921 acctaatagt caaatgacta atattgggta cgcgttaagt cttctttact tcatcctttg
     1981 catgattcgt gtttttaaag caaggtctgc tgtatttctt gtggatttag gatggttgtt
     2041 atcgtttact tgctattttt cattgaaatc gtctttcttt accgtttctt tagtttttta
     2101 tctatttttt tcttttggtt agaaaattct ttttcccgaa agtcttcttt caggattttt
     2161 atagagtagc gttttgaata agaggcgtca gtatgatcga cttggcagac gaaccaagag
     2221 aacatttgcc ttttttctgc atgaggtgct tctgctaata attctttcca taaataataa
     2281 cagtcctcta tcagaagaag ggttcgttca tcctcaggct cccaatagaa tgtttcgaga
     2341 gtagcaatca cttcattgat caattcgaat gcgggaagat aggcattttg ctgaataagc
     2401 gattcaactg cttcttggat gaactttcgc atctccatga tgaacttcgc tgttttcctg
     2461 tttttgtttt tcttgataat atgatcgtat ttatggatga tagttgttaa tttatctgaa
     2521 taatttattg catgaaatgg catcttaact ttgaagcgtt cgataaagtg cggatgttct
     2581 ataagaatct ctgttaaaag ctcccgtaca agagaggaac tagcggagga aacaagggac
     2641 gagagttcgg aaagtgagta gttggatttt tttattggac gttcttcgta agcgaataag
     2701 acagcagcca tgtgtttgca tttttttcct tctatggcat atagacaatc acaagtcatg
     2761 ttggtctttt catcccaagt gatcactacc ttataatctt ccgtgccaga aactgttgcg
     2821 gacaagtact tgcttgtata gttcaaatga cggacaaaac cgtgcagatg atagctgtaa
     2881 ccgcgttcca aaacgttttc aggaaaatac tcaagccaat tattcatcta gttctccttt
     2941 catgaaacac ttatttatat ttagtataaa gaaacgttta agaaaaaagt agctgatttt
     3001 acatatttga taatcacttt tgctcgaatt gtcaaaaatt cattctcctt ttcttttctt
     3061 agagaatgtg agaaaaagta aaagaatagc gtgttttaaa gatggaactt tttttgtttt
     3121 ttatttatct gacaaaaatg aagtgatttt gttagaaata aataattgtt ccaattgata
     3181 aaagaggtat gatgtatcgc aaagaaaaat ccaagcagcg ttctgatcag cagtttgaac
     3241 aacaatttgc tttgtacagt aggtagtaag cggattaact aagggaataa aagatatttt
     3301 taccgctttg tttacttaga atctgtcttc cccaaaattt atttttctaa aggtaaaaca
     3361 tagataggca gataatacca attatccgtt acgaaacgct gcttgagaat tttctttaga
     3421 tccatacaac attttctaca tctgactaaa ttggttcctg attcatcaag taattgactt
     3481 tctttgataa tatgataaaa aggcaatcaa ggaggaagat aaatatgctt aaagatctga
     3541 accaattgat cgattacatc gaagaacatt tgacagaagg actttcctat aaaataatag
     3601 ccgaaaatac aggaatctct gaatatcaat taaaacgcat ctttacgttt attgcaggga
     3661 tgtcgctaat ggactatatc aaaaaccgta gattggcttt ggctaatcgc gatcttctag
     3721 atggatcaag agtgacagaa acggcgttta gatatggata tgaaacagtc gaaggttttt
     3781 caagagcttt tcgtgattgg tcaggatatc tgccttcaga aatcagtaga tacggcatac
     3841 aaaaatcttt tccacgcttg acatttttta ttgatgtcag aggaggaaaa tcgatggaat
     3901 ttcgtatcga aaagaaagaa cagttttatg tggcaggtgt ggcgaaaaaa gttcctgtca
     3961 aatttgaagg agtcaatcaa gaaatacaaa agttagcgca gacgatcacg gagactcaaa
     4021 gggaagagat gcataagtta ggagatttat atccaaatca agtaatcaac gtttcttatg
     4081 gatttgatgg agaccgcttg gaagaaaagg gcagtttgat gcacatgatt ggctttgcta
     4141 cgacaaaaga aaatccgtac gctgatttag aaatgcttgc tatagaagaa cacacttggg
     4201 ccatttttcc taataaaggg ccatttcccc aaactttaca agatacttgg gggaaaatct
     4261 atgcagaatg gcttccttct tcagattatg ggttagtcga agctcccgag atctctttta
     4321 caaaatgggg agaggatttc agtaatgttt acagtgagat ttggattgca gtgaaagcaa
     4381 aaaaatagtt aaaaaataaa aatatcccta gaaaaaagag cgtaatcgtg tcatttccct
     4441 ttaattttat gtaagatgaa cttaatataa tagaaagggt gggagaaatg gcaaagtata
     4501 ggatcgaaga aaaaccagat tttacggtga cgggtattgg tgtggaattg aagagtagca
     4561 atacggattt tgtcggtttg gctgcagaga aacaagcatt tgagcaaaaa gttatcggta
     4621 atggaacaat agatgcattg aaacaagctg ctgaaaatca ttatttcttt acagtcaacg
     4681 aaccctataa cgggaaaatg atgtactatg tcggtgttca aacgagtcag gatgttccaa
     4741 acactacgcg aatcatccca ttcccaaaag gaacgtatct tgttgtaagc gctttagcag
     4801 aatcgcctga agaattaaga aatgatttga cgaatacggc ctttgggcat gttttaagag
     4861 aagtagaaga ttacgcctat gttgggggtc caaatgctgt agtcgaaatg ggagttacca
     4921 acgggaaata tatgggagaa atctggatac cggtagtcaa taaaccatga taatatagag
     4981 gtcgtgaaag tagcgctttg acctgagcat tagagaggaa aaatgggaaa tagctttcca
     5041 tattttgcat ttttcagaac taatgccgag ggtcagctac ttgaacaccg tttatcaaga
     5101 cgtcatgact tcagcgcttt gacctgagca ttagagagga aaaaggggca tgacaaggct
     5161 tttgtccccc ggagaataaa agaatttatg tagacgattc cgatcagaaa aatgcaggca
     5221 cagtagagag aattataaaa atctctctac gaggcctgtg ttttctggtc ttttttcttt
     5281 ttccaaaacg gctattttat gtatcagact atcaagtgtg atcttagcca tttcttcttc
     5341 tgcctttttc tgcagccgag cgtagctttc ttctaaagct tcttggatat tcgcaccaac
     5401 gacgcaattt ggatttgttt ttggatcgac ttggataagc tgtgtattac cttcgatgct
     5461 gcgatagaca tctagtaaag aaatctgatc tggacttttg gcaagggaag gctgcggttt
     5521 tccggttttt gtaaagatca aaccggattt tttgagctga ctcatgattt tacgtacatt
     5581 tgctgcattt gtttcgatac tactggcaat catctcacta gaaagtgatc gggtatcttg
     5641 aaacatttca atataggcaa gtacatggat tgcatcacta aattgcagag aatacttcat
     5701 cagttgtcat cctttcattt tttttgcttg acttatctta ccacactctt tatgataaag
     5761 gtgtaattta taaatgtaca gctgtaaatc ttaacaaata aaaagaatag ggagtggcaa
     5821 atatgcatta tttagtaaca ggagcaactg gaggttttgg tggttatgcg ctggaattct
     5881 taaaaaagat ggttccacaa aatgaaatct atgtactagt acgtagtgaa gaaaaaggag
     5941 aagcactgaa agaagctgga tttaatatac gtattggtga ttatgatgat ttagactcaa
     6001 tgaaagaagc gttgaaagga atagatcgat tgctttttgt ctctggcgtt ccaggaaatc
     6061 gtcaagccga acataagaat gtagtgaagg cagcacaagc agctggggta tcttatatcg
     6121 cttatacaag ttttgcagat gccgatcact cgatcagtgt attagcgcca gatcaccaat
     6181 tcactgaaaa aatgattaag gattcaggta tttcccatac atttttacgg aataattggt
     6241 atgtagagaa tgaattacct atcattgggc aggctttaaa aactggtcaa ttcgtttatg
     6301 ctgcaggaaa tggaaaaaca ggttgggcat tgaaacgtga atatgcagaa gctgcagcaa
     6361 aagtactagt agaaagtgac tctccagaaa tcttagaatt atccggcaat ctaactgact
     6421 atgaagaatt agccaaggca ttagagcgtg cgacaggtaa agagttagaa attattgaag
     6481 caagtgatgc agcgtttgtc gaaaatctaa aagaagccgg attccctcaa gaagcagctg
     6541 acatgttttt agcgatccaa catgatatta aaaaggatca gttagctatt tcctctgatg
     6601 atttagaaaa agtattggga aaaccattaa cttctcttga agcagcatta aaagaaatat
     6661 tggatcagga ataatgtata aagaggttgt gaaaaagccg attagttttg agtatgaaga
     6721 aaaattttgt gaaaaagctg ttctgcatca agtaataaga agagtcaaat aaaaaatagc
     6781 tcttcatatg tttcgtttag ctggacttat taccgcggag ttggcaggag aacgccgttt
     6841 ttattcggaa aaaggagctg agacaagatt tttgtctcag ctcctgtttg tttatcgata
     6901 aaaaagtctg gggaagacga tggctcaggc tttgttggaa acagagccga ttttatcatt
     6961 tgcttttaca tcacgtgcgg gttcgatttc taagctttgg actttgtcgc catttgtgaa
     7021 tacaacgacc atatccgtgt ttttgcctgc agattgaata gcggcaagat caactaaagc
     7081 aatcaattga cctcttgcga ctttttgacc ttcttctaca tataaagtga atggttcgcc
     7141 ttttaagtca acagtgttga tgcccatatg aagcaagact tcgataccag catctgtttg
     7201 gattccgaca gcatgtttcg ttgggaaaat gtttgtgatc gtaccagcga ttggagagaa
     7261 gatttctcca ttttctggta atacagcata gccgtcaccc atcatttttt ccgcgaatac
     7321 atcatctgaa acttcagtga ttggaatcag tttcccattt gcgatagcgt aaatatcttg
     7381 ttcgatcgtt tcaccggcaa atgtttctgt tttaaccacg gtgtttgttt gtgcaggtcg
     7441 tgctgcagca ccaccatgta gttttgccat ctcatctgca acgaattgta cttctgtacc
     7501 tacgatgact tgcgcatttt ttccgtcgat gatcttcata ccaggtacgc cagtggcttt
     7561 gattttattt tgatcgatca ggcctgtatc tttgacttgc aaacgaaggc gtgtagtaca
     7621 gttatcgatc gaagttacat tgtcggctcc gcctaatgca gcgtagattt tgcttgcttg
     7681 tgccgcatat ttattgtctg aattagtcgt tgcagtatcg tcatctgaaa gatctgcttc
     7741 ctcgcggcca ggagtcatca gatggaattt tttgatagca aagttaaagc caaagtagta
     7801 aatcactgct gttactaatc cttggacgat caacatcaat ggctgattcg caatcggatt
     7861 tttcaaagat aggaagtaat cgacaaatcc ggcactaaag gcaaatcctg ctgtccaatg
     7921 gaagaatgca ctcactgcta aagaaatccc agtgaaaatt gcatgcaaga catataatgg
     7981 ccaagcaacg aacataaatg aaaattcaag tggttctgtc acaccagtaa agaatgaagc
     8041 aaaagctgct gctagcatca aggaagctgt ttttgctttg ttttctggac gtgcatttct
     8101 gtaaatagca tatgctcctg ctggaagccc gaacatcatg attgggaaaa atccagcttg
     8161 atacattcct gttacgcctt ttgttccttc agaagccaaa aatttaccaa tatcattgat
     8221 tcctgctacg tcaaaccaga acacggagtt cattgcttga tgcaatcctg tagggatcag
     8281 taaacggttg aagaagccat aaagacctgc accaccccag cctaaactca aaatgaattt
     8341 agcaaaagaa actaaagcag agtaaaccgg aggccatacg agcagtagca ctgcactgat
     8401 cagcaacatg acaaaggcag ccaagattgg cactagacgc ttgccgctaa agaaagataa
     8461 agccatcggt aattttgctt cgtggaaacg attataaaga gctgccgcaa tcaatccggc
     8521 aataatacca ataaatacgt tactgatctg agcaaaagat ggatcaactg attctggttt
     8581 gatattcaat aaagtagcta ctgaatcagt ttttaaaaca tttactggaa cttcaaatgc
     8641 gaccaaacct gccaaggcag cagcacctga tttgtccact gacatcccta gtgctaaccc
     8701 aacggcaaac agcaaaggta attgacctaa aactgcgtta ccgccagctt gtaaaaaatg
     8761 agcgacaatg tcgttactat aagatgccca ccagtttccg atccctacta gtaaagcagc
     8821 agcaggtaag accgcaactg gaagttgaag ggaacgtccc attcgttgta gatatgcttt
     8881 caattttttt cctcctcatg attgttaaca tttgtttgta cgattgcgct tcttaaacgc
     8941 tcgatatgga tggccaagta acccagttct tctttcgtga tcgcgagctg gtattctttg
     9001 gtcagcaaca cacggatttt tttagaaatc ttatagcttt ctgggtattt tcgtttgacc
     9061 atttctacga tttccgtgtc taaaacagca tattgttgcg catgggttcg ttgtagaagc
     9121 agtcgtaaat gattcaccag tcttgaataa ttcagtgaaa atggttctga atggatatct
     9181 gtttccaatt ctgattccac taaatggatg atgtcagata caatcgtcac ttcacggata
     9241 ctgcgatggt tattgttccg accgcttcgt ccgctgtgaa tatggattgc gatatatcct
     9301 gcttcgtcat aagtatatgg aatcccaagc tcttttgtca gatactctac tgcccattga
     9361 gcaatactga attcatcact gtaaagcact tcgatttctc ttaaaagttt gttgcggatg
     9421 acgataccgt tattaatgtt ctccgcagaa aaagcaatgt ggtcggtcaa agcgatcaat
     9481 acatgttcat tcaacttttc catcaaaaca gtttccgcat gatcaatgat cgtttctgct
     9541 gcaaataaaa agcgttcatc aatctgatta agtaaatttt gcaacttgat ttgaccttct
     9601 ggttccatga caaatatccg ttcgacatca cgatcaaaaa tcaaatcatt tctttttctg
     9661 tcaaatccaa tcccttttcc aatagctact ttttcctgtc caccgtcatc taccaatact
     9721 gcattttgat taaagatctt tttgattttc atgcgcgcac cctttttgaa gatttggttt
     9781 tcacgtttgc tagaccagtt gattgcggcc aaacaaaaat agacacgaaa aacctcctag
     9841 ggaaaccatg ttcctcagag atttttcatg cctagtatga tctagtcaca tgaaacgaat
     9901 tattgttaac gtaatcaatt ttatatagac agttatgttt tgtcaattct ttttttataa
     9961 ttatagtaat ttactggaat tatcagaaat aaaaggaaga aataaattaa gaaacttatg
    10021 ttatttaatc gttggatctt ttgcttatct tattcgttgt taaaaagctg aaagcgaagg
    10081 aaaaaaactc tacttaactg tatttctccg ttaggacatc taaaaattta cgagcagcag
    10141 gagaaagagg ctggtctttt ttccaaatga gattcagtga agcttgtaat ggcggatcga
    10201 gtgggatgaa tattttttcc gtcccataag tattgatgat cccatctaga gctaatgcac
    10261 caaaagtccc gttatcgacc atcaaagaag cattgtacaa taagttatat gttccgacga
    10321 tcgataactg atctatgctg aaacctagcc attcagctag ttgactgtcc actgacgttt
    10381 gccgggaaat gatcaaagga acagttttca gttcctgcgg atggatgacg gtttgctttg
    10441 ctaaaggatg atttcgatgt atcaaaagtc cccaacgatc ttgggctggc agtcgaaatg
    10501 actggtattt ttgtttgttt gtcggttcga ttacgagtcc aaaatcaagt aatccgctat
    10561 ccagtttgtc tatgatatca tctgcattcc cgctatataa atgaaactgg atatcaggat
    10621 gcgccttgat caacgaatga aggctatcag aaatgaaaga gattgcttta gtttcgcctc
    10681 cgccaatgta gatttctcca gagatgattg cgtcatttgt taaattgttc gccgtttttt
    10741 ctaccaacga caagatttct tttccttttg aataaagata tcgaccagaa tccgtcaact
    10801 gtatctctcg ggagccccga gtgaaaagtg tcacaccaag ttcttcttct aaatctttca
    10861 gttgttttga gagagtaggt tgcgtgatat gcagagattt tgctgctcgg ctgatatttt
    10921 tttctctagc tactgctaaa aaatacgtca gtatacgaat atccattttt tccctcctgt
    10981 ttttaaaaaa gcataccact aagtatagtt aaaaggaata gttaagtatg ttttataagt
    11041 attagacatg ataagccgtc cgttataaag taaggataaa aggagggagc aaaatgacaa
    11101 gcctcatcga aaaacaaatc gtcaataaag agattcgagt aacggatccg ctctttgaag
    11161 tgatccatca gatccaagct gaaaacgaag agcctttagc agcgctgaat actgggtacc
    11221 atgaaccagc agatatccgc aaacggctag aaaatatcat tagtgacaaa gtagatgata
    11281 cggtgactgt tctgctgcct ttttatacag atttcggcaa acatattagt atagggaaaa
    11341 atgtctttat caatcgtcaa gtgatgttcg ttgacttagg tggcatctgt ttagaagatt
    11401 cagtattgat tggtcctcgt gtcaacctca tcacggtgaa ccatctaacc gatccaaaag
    11461 aacgaagagg cttgtcagta aagccaatcc acatcaagaa aaatgcttgg atcggagcag
    11521 gagcaactat tctgccaggc gtaacgattg gcgagaatgc cattgttgca gcgaatgcaa
    11581 ctgttacgaa agatgtccca gacaatacca tcgttgcagg aatccctgcc aaaatcgtca
    11641 aacctgtcga acggattatt tgaacagtaa gatgaaataa gagatagtga gtataagcaa
    11701 gagatttttc aaaacaggag gagaatagaa tgaatgattt atcagaaaaa gtgattgtca
    11761 tcatgggcgc ttcaagcgga atcggtgaag caacaacgaa gctattagca gaaaaagggg
    11821 ctaaactagt tatagctgca cgccgagaag atcgcctaaa agcaataaaa gaatctttac
    11881 cagaagctga gctatatatt caaacagcag atgtaagaga ttttgcacaa gtccaagcag
    11941 ttatcgattt agcgatggaa aagttcggtc gaatcgatgt attgtacaat aatgcaggaa
    12001 tcatgccaac cgctccatta gttgaaggtc accgtgatga atggcaaaat atgcttgata
    12061 tcaacatcat gggtgtgtta aatggaattt ccgccgtact tccaatcatg gaaaaacaaa
    12121 aatctgggca tatcatttca actgattctg tggcgggaca tgttgtttat cctgattccg
    12181 ccgtttattg cggaacaaaa tttgctgtcc gtgcaatcat ggaaggatta cgtcaagagc
    12241 aacggcaaaa taacatcaaa tctacgatca tctctccagg agctgttcaa actgaattat
    12301 atcaaaccat cagcgacaaa aaggctgctc ttgaacttca tgaagcgcaa aaagaatggg
    12361 gattgacctc cgaagatata gcttctgctg tagcatttgc gatagaaacg ccagaccgga
    12421 tgtctgtcag taacatgatt atccgtccga cgacacagga agtataatcc ataacggata
    12481 atatccaaag agaattactt gttaaatagt tattcaaacg accatcaatt taaggcgtga
    12541 atggagaaga atattcttca tttacgtctt tttatgtaga aaaagcaaat ttatcaaaaa
    12601 gaaacaaatg ttatttaaat atggacattg acttgctttt acttttttag ttacactgtt
    12661 atttaggaaa agtttcataa ttcaaaggag aaaaaagatg aaagcaaaga atttattatt
    12721 tagcgttatt cttttaggaa atctagcagt tgccaacaca acagtgctag ctgaagaatc
    12781 gacagaaact gtcgcaactc agacatcgag ttcttcagaa acaggaattg aaaacgatac
    12841 agagagtact ttgcagtcag ctactgtctc agaaaataca atttcaaccg aagaaccaac
    12901 aactgaaaca acagaacaaa cacaaaccga agacagtaca tcagcagaag ctcagaacaa
    12961 tccagagaat gcagaagaaa tcacgaacca attgacagat gggcaaacat tgacacatac
    13021 ccatcaagca gatttgtctg aattcgaaaa ctatactggc tatgcttttt atgctcgctc
    13081 atctgctaat tctcaacaag cgttcattga tagtattgcc tcaactgcac aaagtttggc
    13141 aagtgccaat gacttatatg catcagtcat gattgctcaa gccatcgttg aaagtggctg
    13201 ggggaacagt actttggctt ctgcccctaa ttacaatcta tttgggatta aaggaagcta
    13261 taatggccaa tctgttacga tgcctacttc ggaatatgtg aatgggcaat ggattacggt
    13321 gaatgccgct tttagaaaat atccttcgta caaagaatca ttgcaagata acgtaacggt
    13381 attgaagacg acttcttttc agccaggcgt ttactactat tccggtgcat ggaaaagcaa
    13441 tacgaactct tacaaagatg ctacggcttg gctaacaggt cgttatgcca ctgcgcctaa
    13501 ttatggttct acattgaaca atgtaatcga aacctataat ctgacacagt atgatacagc
    13561 accaacttca acttctccga tgtatcgtct gtataaccgt catacaggag aacatttata
    13621 tacattgaat gccggagaaa aaaattatct gccaactgta ggctgggaat atgaagggat
    13681 tgcttggcaa gcaccaaaca gcggacaacc tgtctaccgt ttatacaatc cttacagtgg
    13741 agaccatcat tacacaatgg cacaatctga gatcaactct ttaacaaaaa tcggttggcg
    13801 ttatgaaggt ctaagttttt attctggtgg ttccaagcca atttatcgac tatttaatcc
    13861 aaatgaaaaa actggtaccc atcactatac cttgagtgct aaggaaagag attggctaac
    13921 accgatgggc tggagatatg aaggaatagg attttatggt tattaaaaag aggtagtgaa
    13981 aaagctgccc tgcatcaagt agtaagaaca gccaaacaaa aacagatttt catgtttttt
    14041 gtttggttga acttattacc gcagatgcaa gtttttgaac accgtttact caaaaaaggt
    14101 tgtgacaaca tcttgtcata acttttttat tactgtttaa gcatactgat ccaatttcta
    14161 atattcgacg ggattcttgc aaaaggcgat ttcggaaatt tttggcggtg taaaattttc
    14221 tactgcagaa gagctatctt ctatagtttg ataagaatca ataatcttgg ttttagcgtt
    14281 gtgctttctt tatcattttt tccgttcatt ctacaaaaaa tttcttttct ctatactttt
    14341 ctattgagag cgtttttttt attacacagc tgattctttg acttttttct ttttttattc
    14401 tattattgac aagtcaatcg ttgacgagtc aattattaga aaggaggaaa ttatgtcttt
    14461 agaagagatt agtcaccttt tatatcaaat caaattagcc gatcaaaaaa tcgtccagat
    14521 ctttgaagaa aatatcggta taagtcttac tagatacgag atcatgatga gactgaaaga
    14581 agaaaatcca ttgctacaaa gcagtttgca agaaaagttg cagattgatc aaggagcagt
    14641 tactcgtcat ctgaaggtat tggaagcaaa tggctatgtt tcgcgtcaga gaaatcctca
    14701 gaacaaccgg gaagtgtatg tagcgctaac agaaaaagcc cagaagaaaa tcgagcattg
    14761 tgaaatggac cataaagata caggttccgt gctatccccg gctttcagcg atgtagaaat
    14821 gaaacaattt ctaacacttt tagataaatt gaatacccaa ctttgcaata taacaacaga
    14881 aacgaaagag gttaataata atggaacaaa aaaatgattt ttcaacaatt atgactggta
    14941 gaagatccgt tcgcgtatat gatgaaacat acaaaatccc acacgaagaa atgctggaaa
    15001 tgatcaaaga agcgacgact gcaccttctt ctgtcaatat gcaaccatgg cgctttgtgg
    15061 tcgctgaaag cgaggaagca aaagaaatct tgcgtccttt gatccgtttc aatacgagac
    15121 aaaatgatac ttcttctgca atggtcatga tttttggcga tatgcaatgt tacgaatatg
    15181 gcgaagaaat ctataaccaa gcttacgaat cagggaagat gccaaaagaa gtcagagatc
    15241 aacaattagc tgcgatcatt ccttattaca agagtttttc aagagaagaa atgaacgatg
    15301 tagtcaaagt cgattctagc ttagcggcta tgcaattcat gttagttgcg cgtgatcatg
    15361 gctatgaaac aaatccaatt ggtggattcg aagcagatca gttggcagag gcatttggtt
    15421 tggataaaga ccgttatgtt ccagttatta ttttatcaat aggaaaagcg gtagaagaag
    15481 gttacgagtc tgttcgttta gcaccagaaa agatcactac gttcgaataa aaggttttca
    15541 gttactattc agtagctatt gctttttttg aatcgtgtgt ggtaacctaa taaaaaagga
    15601 ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa cttttgaata acaactaaat
    15661 aaattcaaaa gacatgtata aacaacaatt tcatggatgg agtatgtcca ttttttcgaa
    15721 cgacagtaga ataggtgaaa gctatgacag atttggtgat ttagtcatag ttatttcgtt
    15781 cagagtagga aaagatgtga tgcagttttt ttgcaaacag atttttctgc tcttttttag
    15841 tatcgataat tgttgtttta catgaaatag gagtgtaaaa catatggaaa atttagcagt
    15901 aaatataaca aatctacaag tcagctttgg caaccagcta gaactatcta ttgattctct
    15961 tcgtgtctat cagcaagatc gtatagggat cataggagaa aatggtgtcg gtaaatcgac
    16021 tttgctcaaa ctaatagccg gtgaactttt tcccgatcat gggaaaatcc aaacagagat
    16081 caccttcaac tacctgcctc aattaaccta tctcgctgaa gcaaaggacc taaatttgga
    16141 attagccagt catttccagt taagactgga agaaacttca gagcggaaat ggagcggagg
    16201 ggaagaacga aagatcgagt tgatacgtct tctttcttct tatgaacaag ggatgcttct
    16261 agacgagcca acaacccatc tagatagaaa aagtattgat cgactgattg aagagcttcg
    16321 ttattattat ggtacgctgg tttttgttag tcatgatcgc tattttctag atgagctagc
    16381 atcgaaaatc tgggaagtaa aagacggaga aatccgagag ttttcgggga attatagtgc
    16441 ctatctcact caaaaggaat tggagaaaaa gactcagcta cgagaagcag agtcgatcat
    16501 gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag aaaaaacaag cggaaaagtt
    16561 ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga ccggatcggt tgtcttcctc
    16621 taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat gcgaaaacat tagagagaag
    16681 actccaaaaa ataggagaaa caaccaagcc gcagcagatg aaacaaatcc gttttccagt
    16741 accaaaatct cttgaactcc acagccgcta tcccatcatg ggacaaaatg tccaattgga
    16801 aagaagtggg agaacattac tggtaaatgg tgattttcag ttttctttag gtaaaaaaat
    16861 cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta ttagaacata tccgcaaaca
    16921 aggagaagga atccttctct ctccgaaagt aagctttcaa gtatatcagc aaaagggtta
    16981 tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg agacaaacag agttttcaga
    17041 atcgcttgtc cgtagtttgc tgaatcactt agggtttgct caggaaactt tgacgaaacc
    17101 gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg attgctttgc tttttactaa
    17161 gccaagtaat gtgttgctgt tagatgaacc gactaatttt attgatatgg caacgatcga
    17221 agctttagag aagctaatgc aaatatatcc gggaacgatt ttgtttactt ctcatgattc
    17281 ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata aaagggcaga aaataaaaaa
    17341 agtacttacg agaaattttt aaatgtgcta cgatgatttt cgtgacaaat ttaaaggagt
    17401 ggtacagatt gcctacaaat aaaaaagaag gaataatttt tacaacaatg atgtgttttt
    17461 tgatggtctt ggggatgagc gcgtataatt tatggctgca tcaagatctt catttatcaa
    17521 gtttgttgat tggtctagtg cctggattcg tggtggcttt cattttggat gtcttcatca
    17581 tcggtgtgct ggcgaaaaaa attgccttta aactaccagt aaatccagaa agcaaggtcc
    17641 aaatgatctt aactatttca tgcttgatgg ttttaggaat ggtgacatgt atgtctttat
    17701 tcggtgtgat catggaacaa ggaatacctg atcaattatt ctctgcttat atgaatgctt
    17761 ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat catcgttggc ccgctttcaa
    17821 gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa cactgttatt tgaaaaaagg
    17881 ccttggcatg ctttttgtca tagtctcttg aatgcagttc tagaaaaata gtgatccgaa
    17941 cgataggttt tggcctgtcg ttcggatcgc tttttctttt ccaactgtat tgctctttta
    18001 ttgaagataa tttgctcttc aatagatatt tctagaaagg ttctgtttat cccttactca
    18061 aaaaactgct gcatttgttc tcttacttgc tgacaccatt ggagttcctg ctgacaaagc
    18121 ataattctgc gctggatcac aacataacgt ccaaattctt gtttcttgtc tagcgagagg
    18181 tcattcaatt ttttttgatt tttttgcatg attttgttca gctgttgttc tctagtgaat
    18241 agcagtccag tagctgttgt tttatcgagc gtcgaaaaag catagatttt tgctagaaat
    18301 tcatcttttt gcgtaggagc gggagtttcc tctttgatcc attcctctac aagagcacgt
    18361 ccttcatcag tcaagtggta gatctttttt tgagagtgct cgtctttgtc caaaacggtc
    18421 acataaccct gctggtctaa cttggcaagc gttgtataga tctgactgtg atgggcttcc
    18481 caaaaaattg tgatcagctg cttcaattca tacccagaac gtggtttgga taaaagcacg
    18541 cataataata tataggctaa agtattcata aaaatcctac ctctcgtctt attttcttta
    18601 ttatatcaca gtaaagtttc cacgttttac atgtgcatat ttacatgtaa aataaatcat
    18661 gtatgatgct aatagagaga aagggatgga aatattggag aatcaaatag atatacaaga
    18721 acaaacaaat gaagaaacaa aggggaaaag ttcagcgcta gttgtagcag ctacctgtat
    18781 gatcgctttt atgggagtgg gtctagtaga cccaatctta aaaacgattg ctgcacagtt
    18841 aaacgctacg ccagctgaaa cgaccctgtt atttactagt tacatgttag tcactggtgt
    18901 ggtgatgcta tttacaggtt tcttatctag tcggatcggt ttgaaaaaac attgatgaca
    18961 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    19021 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    19081 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    19141 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    19201 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    19261 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    19321 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    19381 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    19441 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    19501 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    19561 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    19621 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    19681 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    19741 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    19801 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    19861 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    19921 caagtcataa gaa
//
LOCUS       DACIRY010000265        11582 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.084, whole genome shotgun sequence.
ACCESSION   DACIRY010000265 DACIRY010000000
VERSION     DACIRY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.084"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..151)
                     /locus_tag="IYE22_002819"
     CDS             complement(<1..151)
                     /locus_tag="IYE22_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ9507992.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSIN"
     gene            381..878
                     /gene="dfrG"
                     /locus_tag="IYE22_002820"
     CDS             381..878
                     /gene="dfrG"
                     /locus_tag="IYE22_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ9507993.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(950..2902)
                     /locus_tag="IYE22_002821"
     CDS             complement(950..2902)
                     /locus_tag="IYE22_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ9507994.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2909..3205)
                     /locus_tag="IYE22_002822"
     CDS             complement(2909..3205)
                     /locus_tag="IYE22_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9507995.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3380..4183)
                     /locus_tag="IYE22_002823"
     CDS             complement(3380..4183)
                     /locus_tag="IYE22_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ9507996.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(4199..4315)
                     /locus_tag="IYE22_002824"
     CDS             complement(4199..4315)
                     /locus_tag="IYE22_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ9507997.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(4560..5489)
                     /locus_tag="IYE22_002825"
     CDS             complement(4560..5489)
                     /locus_tag="IYE22_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9507998.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(5506..6528)
                     /locus_tag="IYE22_002826"
     CDS             complement(5506..6528)
                     /locus_tag="IYE22_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ9507999.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(6525..8552)
                     /locus_tag="IYE22_002827"
     CDS             complement(6525..8552)
                     /locus_tag="IYE22_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9508000.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(8549..11002)
                     /locus_tag="IYE22_002828"
     CDS             complement(8549..11002)
                     /locus_tag="IYE22_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ9508001.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(10986..11381)
                     /locus_tag="IYE22_002829"
     CDS             complement(10986..11381)
                     /locus_tag="IYE22_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9508002.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
BASE COUNT         3441 a         2224 c         1970 g         3947 t
ORIGIN      
        1 catttattga agtatacatt tctgtaactt ttattttttc tttttctgat actctaaccg
       61 aatctcgtaa atgtagtact ccactataaa ggcgtatata tgcaagtaag tagttcagtt
      121 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
      181 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
      241 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
      301 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
      361 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
      421 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
      481 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
      541 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
      601 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
      661 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
      721 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
      781 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
      841 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
      901 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
      961 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1021 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     1081 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     1141 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     1201 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     1261 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     1321 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     1381 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     1441 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     1501 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     1561 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     1621 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     1681 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     1741 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     1801 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     1861 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     1921 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     1981 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     2041 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     2101 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     2161 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     2221 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     2281 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     2341 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     2401 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     2461 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     2521 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     2581 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     2641 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     2701 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     2761 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     2821 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     2881 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     2941 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3001 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     3061 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     3121 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     3181 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     3241 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     3301 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     3361 tactccaaaa ctgaactact taatatgcaa gacgttgtct tttttttgta tattcaattt
     3421 tgaaaacatt tccgcaaagt tcagacggac ctcgatgtgt tgatgaataa aatttattag
     3481 taataacttc tataaggtta tcaatcccta tattactttt tgcacttcca tgataaagag
     3541 ggaacagaga acaattctga aatcttatgc tttcctcttg ttcgagttcc aatgcttcta
     3601 atgatttacc ggacatatat ttctctaaaa ggtcatcgtt tccctctatt accgtatccc
     3661 attgttcaga ttcggtaaag ttcgtcacac acatattagg atacagttct accttctgtt
     3721 tgattacaat ttcggcagaa agtttctctt taatatcctg ataaaccgtt gataaatcaa
     3781 ttccattttg gtcaatctta ttgataaaaa agattgtggg aatccccatt ttcctaagtg
     3841 catgaaataa tatacgagtt tgtgcttgta cgccatcttt tgcagaaatc agtagaattg
     3901 ccccatctaa aactgataat gaacgatata cttctgctaa gaaatccata tgtcctggcg
     3961 tgtctatgat gttcaccttc gtattttccc actgaaaaga ggttattcct gtctgaattg
     4021 taattcctct ctgacgttct aaaagcgtat tatccgtcct cgttgtacct ttgtccacgc
     4081 ttcctaattc tgtaatcgct ccactgttat ataataagct ttctgttaag gtagtttttc
     4141 ctgcatcaac atgagctaaa actccaatat taataatttt catgtgattt tcctccattc
     4201 aaaaacccaa aagggcataa aaatcccagt gataaatact tttatcactg ggatttttat
     4261 gcataaccat aggtatacaa agcatacaga tattctctgg atactttaga atcacatgat
     4321 aaaggtattc ttaaactggg tacaaaaaac taagccctcc taaaaaagga catctaatta
     4381 tttgttcccg ctatcaaatt gacagtttat ttaagaatac cttgccgcat atttattaac
     4441 tccttttaaa tagtcactta aataatagca cgtaagagca tatttgtaaa ggaatctcca
     4501 attttttatc aaaaagagta catgattaca aagtatctgt aatcatgtac caatatttgt
     4561 tattttacaa tcttccaatt atcttgtttt tctaatatca actcaaattg cgagatttgg
     4621 gttgcctttg tttcttgatc taaatatttc acagcgacat tcacgattac ttggttatct
     4681 tttctggtaa agataggatt caccaattcc tcgaaaacat attccttgtt aatcataggt
     4741 aacacatgat tacttacata gtaagtcaat tctttttctg tggcggtagg atagagctgg
     4801 aagaacgttt ctaaaaagct agtgatttcg tccatcgttt ctgtatctac tgtatggtca
     4861 ctttctagct gtttgggttg gtagtccgat ttttgaggtt tcttactcat tgtcggattt
     4921 ttgataataa ccatattatc tgattcatct atatggacaa ccacatgaaa gctagacgat
     4981 atagtttcct tgtctttgtc ttcggtaata acttgttcaa cagaaaacag tacctcaaag
     5041 gtgttctcat tcacttgaga aacctgccac acctgtatat cgttcacact agaactagta
     5101 ggaatatcct tacgaatcat ttcttcattt aatacctgca attcttccgt caaataatgg
     5161 gtcaattttt catttcgttt atcaatggct tcttgggatt gttgccacga aaagtagtct
     5221 ttcgcaaagg atttcacaaa actttctact tggttggtat cgacaatctg ctgtttaatg
     5281 atttctgttt cacggacagt atgagtgtca atggcagtaa aatttttata gattccaaat
     5341 ccgacactac caatcaataa aacccataaa acaaaggtta atttcttatg cgtgccaact
     5401 ttaatcatcg gaacttttct ttgcttgctc ggttttttag atttctttgg tttctccttc
     5461 ttttcttttc gctcgatttt tataatcatt tgattcattc ctttcttagt tatgaataac
     5521 acgtctagcc ccaatcagat ggtcttgcca ataacgactg cttaagtcgc cataaccgat
     5581 tgggtcgcct gcatgataaa agcgatttcc ctctaaatat atcgccacat gagtcacata
     5641 tgtgcctgca ttataggttg aatgaaagaa gataaggtcg cctgcttggg cttcttccat
     5701 agagatttct tgcgtagcgt catactgatc ttgggcgaca cgagggagag aaatgccagc
     5761 tttgtcatac acccattgta ttaagccact acaatcaaaa gaggtggtag gcgacgcccc
     5821 tccaaagaca tagggaaacc cttcgtattt caaggcttcg tccataatga cttgcacggt
     5881 ttcatcatca aattctgttg gggaagtgtc ggttaggtat tgtgaaacca actgaacata
     5941 aaactgattt ccatagttat atcgccaacc accattcaca ggtatggcta taggattggg
     6001 atagtctgct ttttgaccac ccgacttctc tttagaaaac tgttcggcta attcataggt
     6061 atattttttc ccatgactcc tcacataatt taaaaagcca ccgccataat tataagattg
     6121 gataacagaa tctatatcca ctccttgttg ttctgcactg gttaataatt cactgaaata
     6181 tttaacccct tgtttgatgg actcttcggt actcaaactg ttgggcggta aacccaaaga
     6241 ttcggaactt tgcataacat cttctgctgt accgcccgat tccacttgca taatggctaa
     6301 gatataagaa acatagtctt caatcccata ttccttacca tatttttcaa tcaacggacg
     6361 gtgtgccaac acttctggtg aaacggtcac accgccttgt ggaatagaag aatccttgtt
     6421 gttttcgcca ctgtccgtat catctgcaaa gaaaatgact aggaataata agagaaaaaa
     6481 gaagagagag aagaccacac tacaaatcac tgttattttt agcttcatgt ttgttgtcct
     6541 cgcttatccg ttcttttatt tagcttcctc tgattgaatt gcttcggtgg tgtcttttgt
     6601 ttttgctctt ttttcaatgg tcgctgatga ttttcacgtt tagtgttttg tgacaaaaca
     6661 ggcgtgacgg tttgctgacg cttgcgaaca tgctgctggt tattctgaac aggagatgat
     6721 gggttttctt ttcgtctttt ttcttttgct ttatccagct ccaaacgctt ttgagcaatg
     6781 ttctttcgtt tttgctcctg ctggttcata cgctgttgtt tgcgttgagc tttggtatct
     6841 gccatagatt gtttaaagtc tgttacatta tgagtgacat tctcttttcc ttgatgaagg
     6901 gcatatttga catgggttgg tgtcgcctgt atctgttgtt ttgcctgctt tcttttattg
     6961 actagacgat ttttcgtgtc caatacatgg gctgtctttt cacctaatcg ttgcccgata
     7021 ccgccttgtt gctttttcac attggaaagt gctgtttctt tctttggtgt agtccgttca
     7081 ttttgacgag tatggttagc tttattggtt tgattctgct ttgttgtcat tgtcttttgc
     7141 ttcgttgacc ctgcttttcc acgaaaaatc ttactcatac tgcgttgaaa tcttctggtt
     7201 tgtcgattca tcatttgacg aggtctgcga aacacttgtc tgcccatgtt ttgagagtcg
     7261 ttgctttgta aagtgaatag gctcattaaa tcgcccagtt taaaataaat ccctgcaaag
     7321 gtcacaattt gtaaaaacat aatcatgaaa aagggagaac ttgctgacag cgaataaagc
     7381 atagtggaaa tactaaaggc aacggtaata atcaaggtaa taccagccct tgtcataatc
     7441 acattaaata acttggtaat ggcatgcctg cccatgccat caaaactcgg caacatactt
     7501 aataggaaac tgattggcaa aaacatagcg aaaataataa atagcacttg cgaaaaaatc
     7561 atgattcctg ttagaaggaa aacaaaaatc gagataccaa tattgaatac aaacaaaaag
     7621 aagactgttc ctaatctgga aatcgtcttc ggtagggtta aataggtatt gtcatgatct
     7681 tcaatttctt caatcactac attttctcta tcttcaccat tgtttgtatc tggactggta
     7741 gacaataaac tttccacacg ttcttcccct aacgcttcta catcagtgga gccatattga
     7801 agcaatagcc atggttgctt tacttgaaca gagaataagc tgtcacgtat catgtccaca
     7861 ctgtctttcc cttgactatc cgtgttagga agaacaattt ttgtcccaat attcagactg
     7921 gcggtactga catctttgga aaaatcattg attttcgcaa tataatcggg tgcatacgca
     7981 ataaaggaag ccgatagaat aaacaccacg acaaagttta aaacagctcg tatcactttc
     8041 gtcgtttctc gcttaaccaa tcctgtatag gcaacataga ttccaacaat gagaataaaa
     8101 agaaggagga aaccgacata aaaccctgaa ctggaaaagc cattcggtgt aatgcctgca
     8161 atcgtctgca tgttttcgcc aattgcttgg gatgtttgac taatgaaatc taaggcatag
     8221 gcttctttca ccaaataacc tgtggcgttc gatagataaa gagaaattgt ccaaataaaa
     8281 ttagtaatgg catacaatcc atacatcacc gattgtccta tgccatcact ccaattccat
     8341 ggaagccagc cccagctatt gtccacataa taatcaagct gatattgatt caaaccatac
     8401 ttggagtatt cattgcttgt atcaatggtt tcatcaacta agcctgtcgc ttgtactgca
     8461 ttgcccagcg tgcctaataa aagtaaaata acgcccacac aaaggagcgt tatccaacta
     8521 tagtgaagaa atttcttttg tttttgtgtc attcttccac ctcatttcgc acaggcggtc
     8581 tggtatcaaa ggcatgaagc aactcttcaa aaatcgggtg aaactgcata acccctacac
     8641 gaccatagat gtctgaaact aaacattgtc cattttctaa ttcacgtaag cgtttctgat
     8701 tgttttcatc ttccttgtcc acaccaaaaa attctaaggt ctttttgatt tccactaggt
     8761 cagtggaacg aaaggcaaat ttcaagccaa tattattctt catcttttca tctagtaaat
     8821 cgtccgcgtt ttgagtaacg aaatagaccc ctgcattcat ggcacgtcct gctcgaacca
     8881 atcgcataga tagagctttt ccttgtgcca cttgtaaaaa actccaagcc tcatcaaggt
     8941 caacaatttt aaaaatggaa cggtcagagt ggataaagtc taaagcaaag gtactaatga
     9001 caatcagcat tgccacactt aataattcca tggtagtgta ttcttcaaaa gagctattgg
     9061 aatcgggtaa gaccaaatct gccacttgaa taatattgag ttgtttttcc aagctaatgg
     9121 attgggtgac actgccatca ctgaataaca gatgggcaaa atcataatcg acaaagcttt
     9181 cgatatggtc agcaatacta ttgctgatgg gcgtatcttc ttttcgcaat tcatcaatca
     9241 ccaataacaa ccctcgttgt ttgctttgag ttacactccg aatagcttta cgcaggacag
     9301 gaaacttctc gctatctcgg ctggaaatac ctgttaagaa cgtgagaata tcaatggcaa
     9361 ggctttctga atctttcggg agttttaaaa tcacataagg gtcaagtaac cctttattgt
     9421 cgttctcact cgtgagatta acaatattga tttcatgggc aatatcaggt aaggtttctt
     9481 tccattcacc acgttcagct tttgggtcaa caatggttgc ctgtccgcca aagagtactg
     9541 aataatagac cagcatattg ttagagaatg attttccgcc acctaatgaa ccaataaaag
     9601 cggacgctaa tgcatttgtt acagagcctt taattccttg acttgcaagg ctaggttgga
     9661 gataaatatt tctgcctgta tcgagattgt aaccgacgta aataccagaa ttttcaccaa
     9721 tcatttgtgt agcaccgaaa ccaagaccag ccaaaaaatc cgacgtgaca tattgaatat
     9781 agtcattgat atatcgcttg cttgagggaa taaattcatt atgcaagcct agcatatcgc
     9841 caaaaggtcg tactaatttg atagacagat catcataaaa atcttttact tcatcacaac
     9901 gttgtttcag ctcgtccaaa ttctttgctg atacccgtac cacatagctt agtttgtaca
     9961 tagattcttt ggtttggtct aaattacttt ctaattcatc gacagattct aaagcgtcca
    10021 ccacattagt actggtttcg ttgtcgcttt cccatgcgtg gctatctaaa tctttcagtt
    10081 ctttcttttt attgcgaacg gtagataagg cttttttatt ggcgacaatt tcaacattca
    10141 tactggtatc aacggggaaa gtaaactgct gttgctggta ataaaaaatt tcactattag
    10201 ggaaatccaa ttcaccaaca atactattaa ttgtaaaata cgccacataa gtagtggtat
    10261 cttcctgttc aatccgtaaa tagcgttgat tttcttcgat taaacagcga gtgggtttga
    10321 gaatatcgta tttctttacc aacacttttt tatcttcgta tcgtttcggt aaatggtagt
    10381 cgtagtcatc aaaagggaca cctgtttgcc cgtaaagatg ttcaataata taaccaaaat
    10441 catctttctc caatttacga atctgaaagc gtctggataa tttactttct aacaactgtt
    10501 ccatcttaga aaagcgttcg atttccgcca taggcataga aacaaaatcg cccattaatt
    10561 tgtgattcac accatgaaag aattccgcaa atgctttttg aatttctttc ccaacatttt
    10621 ttacggatac ttcctgttca ttgaggtaga gtttaaaacc aataaaaaag cgatagtcta
    10681 cttgattgtc accaatcata gaaattaacg cttcggtctg ttcatctatt ttttcgtaag
    10741 ctatctcttt taatcgtcca gtgatttctt ttttcgattg ttcctgtgtg gctcgaatac
    10801 ttgattcggt tgctagttgc agagcatgaa tcttgccatc acgattttgg gcaatcaatt
    10861 gacagaaatt atcatgaact tgaatttttt catcggaact taaaaaactg taattatagg
    10921 gaattaattc ataataagcg aatacttcgc catcatgatt aaatacgaga ttatcttcca
    10981 tatatttaat cggatatgcc atctagttgt tcactcctta caatcgtaat cgattcattc
    11041 attgtttctt tttttaatcg tatttgtttg ccagcgtagg ttactttagg gcgtagagca
    11101 taggtaatga tagactttag aaatccatag ggtttctttc cgtcaaaggt cttttggcac
    11161 atgaacccag tgagaacaat gggaatccct aagtatttga gtagtgctcc gtcaatcaga
    11221 gaaagtggtg gtaaactgcc aaagagtatg acgacaaaca aagaaacgat aaaccatgtc
    11281 atttgcgtaa aggtaattgg gaaagggagt tgtaaatcat taatcgcata aatcactttt
    11341 tctactgacc aaatactggt gtaacttctt atttttttca tgtcttcacg tcctttccgt
    11401 aaaaatagaa aaagaacaat caaaataggt gattgctctt tcaatgcttt aactattcaa
    11461 ttttattttt tgtccatttt gcaataatac cttcatcagt ttcttcatca tctggattca
    11521 ttgaatatct gaaacttaaa ttggtatctg accctaatcg ttcatataag ctgggtctat
    11581 ac
//
LOCUS       DACIRY010000266         9058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.109, whole genome shotgun sequence.
ACCESSION   DACIRY010000266 DACIRY010000000
VERSION     DACIRY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.109"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(101..544)
                     /locus_tag="IYE22_002830"
     CDS             complement(101..544)
                     /locus_tag="IYE22_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ9508003.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(739..2079)
                     /locus_tag="IYE22_002831"
     CDS             complement(739..2079)
                     /locus_tag="IYE22_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9508004.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2361..3257
                     /locus_tag="IYE22_002832"
     CDS             2361..3257
                     /locus_tag="IYE22_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ9508005.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3400..4341)
                     /locus_tag="IYE22_002833"
     CDS             complement(3400..4341)
                     /locus_tag="IYE22_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ9508006.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEVF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4467..5588
                     /locus_tag="IYE22_002834"
     CDS             4467..5588
                     /locus_tag="IYE22_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ9508007.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5846..7348
                     /gene="lsa"
                     /locus_tag="IYE22_002835"
     CDS             5846..7348
                     /gene="lsa"
                     /locus_tag="IYE22_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ9508008.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7415..7942
                     /locus_tag="IYE22_002836"
     CDS             7415..7942
                     /locus_tag="IYE22_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ9508009.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            8154..>9058
                     /locus_tag="IYE22_002837"
     CDS             8154..>9058
                     /locus_tag="IYE22_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ9508010.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEK"
BASE COUNT         3023 a         1656 c         1816 g         2563 t
ORIGIN      
        1 cacaacctct gattcacggt gttccagaag tagcttcttc ttatttcttg aagttgatca
       61 cttctggaac atcctctttt tttatttgga tggataatgg tcattccatt tctttgatca
      121 tttttttcat tttgtcgatt tcttcttctt gtgcctgaat gatttcttcc gctaattttt
      181 ttacttcagg atcttttaaa tcagctcgtt tacttgtcaa gatagcaata gagtgatgag
      241 ggatcatcgc cttcatccac gccgtatcgt tgacagttgt ttggcttcgt accataaata
      301 aggaaccggc aaaaacaaga agactagcgg ctaaaatcat agcatttatc tttttgtttt
      361 catacatttt ccacatgaac aacaacatga tgactgccat gactgcaccc atcatcaacg
      421 ccatgaatac acgtgtgcga ctgaagaaga tatgatcaag cgcataaaca ttcaaataca
      481 tcataccaaa catgactacc gtcgaaacac caatcatcaa caaaaacttc caatactttt
      541 tcatttaaaa caaactcctt tttcttcatt cctttcttct atcatgtcat gagcgtggag
      601 aacaatcaat taatctgcac agatggtttt tgaacaccgt ttattcggaa aaaagaactg
      661 caacaatttt tgtcgcagct cctttttttc aatagctcca gatattcaat ataaatagaa
      721 taaaattctc tattggtttc attcattcgt caagacgaaa gcatcattct ttttttgata
      781 actgaaataa atgaacaggt aaagcaaacc ggtcaataca gttaccccaa tggagttcgt
      841 gaataggata ccaaggaaaa tcaaccataa gactgctaat gcaaaaaagc tgattccttt
      901 gtcccataac gttcctttcc ttagtgtcag tacaaaggac gcagcactga taatgatcca
      961 gatcaataat actgtatagg aaagagaacc tgccagatag ccaaataatt tgtcaccgac
     1021 aaaataagaa agagccacac ctagataaag aacactggta caaaacagca cagcacgttg
     1081 cggtacttga tgcttgttta aaacagctag ttttctagag gaacctttcc gatctttcaa
     1141 gcggaagaac aaaattctcg aagaagcata tacacctgaa ttgatagacg agaacagagc
     1201 aagaacaatc acgaaattga cgatatctgc cgcaaatgga atattcattt tttcaaatac
     1261 catcacaaaa ggactaacgt tcgtgcctgc taaagtattc caaggaaaaa taatcaaaag
     1321 caaaaataaa ggaataatgt aaaaggaaat gattcttccc atgacgcctt tgattgcttt
     1381 aggaatcgca cgtttaggat catctgcttc gcttactgtg atagcaatca actcagaacc
     1441 gccgtaagaa tagataacta caagcaaaga actgatgatt cccttcatac cgtggggagt
     1501 aaaaccacca tgatcagtta attgttgaag ggtagggaaa acaccagtac ccaacatttg
     1561 ctgaccgaca agataagcag caaaaatgat caataggata atgacactga ttttagcaaa
     1621 ggccagccaa tattctgtct ctgcgaataa tcgtaccgaa taaagattga tgatagtagt
     1681 taaaacagcc aacataaaaa caaataccca agctgggatg tgggggaacc acaactgtaa
     1741 aaaggaagca gctgcaacag cttcagcaat gatattgata atccacatgg accagtagac
     1801 ccaatcggta aaatcggcag catgagcacc tagatatgga cgaatcaatc cagataatcc
     1861 gtggtgcgta tcaccaccac ttaaaacaat ttttcccaga cctttcatca cgatatataa
     1921 aattatgccg ccaatcaaaa aagcgatcaa tacagaaggg ccggcgattc caatcgcttc
     1981 gccacttcct ttaaatagac ctgcaccaat cgctccacct aaagctagca tggtgatatg
     2041 tcgggagcta agttgttttt tcaatttttt atcttccata aataacctct ttcttttaaa
     2101 ttcacaatca aaatgcatag taagaataaa tagtaagaat aaatagtaat aataataatg
     2161 actaatgata acaaattttc agataattac aactattcag aaaacttttg ggcatatttt
     2221 aatggaagaa tgatttattt catcgtattt tcatagaaat gttaaatttt tataaaaata
     2281 ataggaagcg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
     2341 gagaaagtga ggcggaacag atgaacaaaa acgacgtatt gctaattgcg aaaaataaat
     2401 cactttattt ttttccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
     2461 agtcggaatt cattaaatca gctcgacaac agttggcaaa ttatcagctg atcgtttttt
     2521 tagattatgg atttgatatg gagatggctc gacgcgtgcg tccatttacc aatgcaaaaa
     2581 tcgtgttatt tttttggaac cactttaaag cagaacacga ggcgatgctg aagcaggcac
     2641 aagatgaacc ggcaatcgat gagatttatc attttgattt tttagaagcg aaagaacttg
     2701 gactaaaaca taatagttcc ttttattcga aacatatgga tttttcttct agcgaaccaa
     2761 agacggatct attttttggt gcaacaaata acggcagaaa agaacgggca gaaaaatata
     2821 agcaggaatt tacaaaaaga ggtgttacga ccaattactt tattcttccg cttcgaggaa
     2881 atgaacaacc tggatacctt acttatgaag aatatctgaa aaaaaccggg gaatcgaggg
     2941 ggattctaga actgttgcgg gaagggcagc aaggagtgac tttgcgaacg tttgaatcta
     3001 tgtattttca aaagaaacta gtgacagata atcaagccat agctcattat cagttttatc
     3061 attctgaaaa tatttttttg cttcaagaac gtgatttaga tgaattaccg gaatttttgc
     3121 atacgccatt tcaaccaatc ccatcagaac tgttggaatt ttttgatgca gaaaattggg
     3181 caaaacgttt tttcactact gactccgcta tttatgaaca atatgaatac gatctttcct
     3241 ctgttaaaaa taaataaaag tgccaaccaa tcatagatga aaataaacaa gcgcaattct
     3301 tatggcaaca tgagaattgt gtttttcttt ttgaactttc atgggggtaa aaaaatgaga
     3361 accagctgct gatccagctg attctcatca gagagccttt tattctgcgc caaggattcc
     3421 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     3481 ttgtcctttt cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     3541 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     3601 agcaatatgt tggtacactt cttcttgatc taatgtgatt ccttcttttg ctgcgataga
     3661 agcaaactca gatacaatcg ttcgaaccat gccctctgcc gctttttgcg cacctaattc
     3721 agcaatatta caatccaata tcgtgcataa tccatttaat gttccgttta cacaagcttt
     3781 tcgccaaatg gaataacgaa cattatgact atagattgga ttcaattttg cttcttgaaa
     3841 gacttctaca actttttttg taaatacttc gccttctggt tccaagttct ctagttcgat
     3901 ttctccatca cctaataatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     3961 gatcccaaat aagatatttt tctttggtac atatttttcc agtacatctt catgacctaa
     4021 tccattcagt aaacacaaaa cataagtatt ctcagtaata atggattgga tcgaacgaaa
     4081 catatcatct aattgattcg cttttgtcaa ggctacgatc aaatccacgt gtttgttgct
     4141 ttcgatgatt tcttctggtg aatagattgg cagttttgca actacttctt tgccattaaa
     4201 gtcagcaatc aatccatttg tacgaatggc ttcaatgtga gcaggccagc gatcgatcaa
     4261 gacgacctca tttccgcttt gatgcaacat taatccgatt cggctgccca ttgctccggc
     4321 tccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     4381 tatagaaaaa cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt
     4441 acttaagaag aaagtgaggg atatagatgg atattttggc ggcaatcgat tcatttaaag
     4501 gaagtgcttc ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag
     4561 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     4621 acgcgctcgg gggaacctgg cgtacgattg atgggatgga tctcttattt cgtcagcgaa
     4681 aaatcagtta tttattgacg aactggcaag gaaaaaatac cgcagtcatt gaatctgcac
     4741 aagtattagg attagattta cttgataaac caacggatca aaccatccga cttgcttcta
     4801 gttatgcctt gggagagctt atcaaagata cactgcttca agaagtggaa caaatcattg
     4861 tcactttagg aggaagcgga tgtagcgatg gtggtctagg attactccaa tctttgggtg
     4921 cttctttaga agggtacaca gaaggaaatc ctttgttatc tactagaaaa cttcatttgg
     4981 atcgaaggaa cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc
     5041 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaacggata
     5101 cgacgttagc tttcttggat aaacaagctg aaaagattgc tcagcagata aaagatgttt
     5161 gggaggtgga tttagatgac atcccaggaa gcggagcagc aggcggtatt ggcggagccc
     5221 tttttttgtt aggagcgaag atggtctctg gttttgagtt ggtcagccaa atcactggat
     5281 tagaaaaaca gatcgaacaa gcggatgtca tcattacagg agaagggcgg attgatcagc
     5341 aaacaatcca cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac
     5401 caataatcgc tatttgcgga agtagagacc gagagctgga aggactcttt gattttctcc
     5461 ccattgtctt aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc
     5521 aaacattaga aaaaacagaa atcttaggac agacactcag cagatttttc aacattctcc
     5581 cgaagtagtt gacaaaaaca atttctttat gctatcgtaa tttcaaatat aggacaaagt
     5641 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     5701 tgtggagaca agggagcagt gtgcagattt tcgggattgg cttggttctt tcgatgtatg
     5761 ggtctttttg tgtgccccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     5821 tcttttttta cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg
     5881 ctatgacagc caaggcacat tattatttga acaagccaat ctaaactttg acacacaatg
     5941 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atatcctgca
     6001 aaacaaactc ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca
     6061 gcaaacaaaa gataaagagc gtttgaccta ttatgtgtta aatgagatta tggattttga
     6121 gatatgggaa attgaaagag agctccaact gatgcaaaca gatcctgaaa tcttatggag
     6181 agaattcagc acactatcag gaggagagaa gacaaaagtc ctactggcac tcttatttgt
     6241 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggcag
     6301 aaaacaagta gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga
     6361 tcggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaga gccaactgga
     6421 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     6481 aatagctaaa aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga
     6541 aaaagcagaa tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag tcggattcat
     6601 cgatacggaa tctaggcgag tgaataaagg agcagtaggc gctgatgctg cacggacgat
     6661 gaaacgatct aaagcaatcg tgaatcggat ggaaacccag atcagtgaga aagaaaaatt
     6721 attaaaagat atcgagtata tcgatgcttt gacgatgaat agccaagtgt ctcatcataa
     6781 acggctgtta agcgtagaaa atcttcaatt agggtatgaa aatctgttat tcgagccagt
     6841 tcattttacg atcgaacctc atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa
     6901 gtcatccatt atccattatc ttctggggac attcaacggc aaggttgtag gagaaaaatc
     6961 ccagcccaaa catttgacca ttagttacgt acgccaaaat tatgaagaca atcgagggac
     7021 attggcggaa ttcgcgaaga aaaaccgagt agactatcaa gcatttttga ataatctccg
     7081 aaagcttggg atggaaagag atgtttttca taataaaatc gaacagatga gtatgggaca
     7141 acggaaaaaa gtggaattgg ctaaatcttt atcccaacca gctgagctct atatatggga
     7201 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa
     7261 cgtgaaacct gctatgttac tagtggaaca tgatcaaacc tttctagata aagtatctac
     7321 tgagattatt tctcttgaga aaatctaaac cttgtaatct caaagacaac aaggtatggt
     7381 taaggagaac tttgtcaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     7441 aacaacttgg aacgcacaag gattagtttg cgggcgagca gatgtgccgc tgactgaaaa
     7501 aggacagatg caggcacagg cactagcaga aaaggtagta gatttgcctg ttccaatcac
     7561 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag tagttgatcg
     7621 tctcaatttg ccgatgaccg ctgatgagcg attggtagaa atggattttg gggattatga
     7681 cggactgcca agcaaagatg aaaactttca aaaagcgcgt ttagcgtttg ctgtacgttt
     7741 cccaaatgga gagtcggtct tagatgtgta cgcacggatc gttcctttat tgaaagaatg
     7801 catggaagac gaagaaaatg tctacttgtt agtctgtcat aatgccttga taagagtgat
     7861 caatgcttat tttcatccaa tgcctaatga agggttcttt acattcatgg tagacaatac
     7921 agaattaatt tcttatgagt aattagactt ttgtgttatt taaataaaat gtttcaaggc
     7981 actgcttttg cttagcagtg ccttttttgt ggctgtgtca attcttttgg cattataaat
     8041 ccaattttta gaaataaaat ttaattattt taacaaaagt attgaaattt tgtttcttta
     8101 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     8161 gcttttcaat atttatgaat gattctttgt cagaagagaa gaaacagtat atccgccaaa
     8221 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccggaa gatgatgttt
     8281 cggcttatca aaatcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagagt
     8341 tgatggtcga tatttcagga gaggcattga aacgagctgg cttttcatta gaagatctaa
     8401 cacccttgaa agcaattggt gtaacagggt taagaatgga ttatcatatt tctaatcagc
     8461 agatcgccga ctggtcacat caattgaaaa tcagtttgaa tgccagcacg atcacgccta
     8521 atgatatcga tgagttgaaa gaagcagcgg ctgatttctc acagattgaa gcttggcata
     8581 attattatcc acgtcttgaa acagggttgg acaaagaatg gtatcaggaa aaaaatcaat
     8641 ggctcaaaac tcaaggattc cttgtacaag gctttgtacc aggagataca gagttgagag
     8701 gtccactgta tcaaggatta ccaactttgg aagaacatcg agggatccat cctctggcag
     8761 gtgctcttga tctattagca agtaacacgg atattgttta tatcggagat gcaggtctgt
     8821 cagaacacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     8881 cggaacctgt agatgaagtt ttttacgaat atatattagg aaaacatacg aatcggcaag
     8941 atgatgcaag agatgtcata cgaagcgcag atgcgcgttt tcgagagatt ccgccaatcc
     9001 cagtaagaaa tacagctaag cgtatgaaag gatctattac tttagataac gaaaagta
//
LOCUS       DACIRY010000267         8277 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.117, whole genome shotgun sequence.
ACCESSION   DACIRY010000267 DACIRY010000000
VERSION     DACIRY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.117"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            10..285
                     /locus_tag="IYE22_002838"
     CDS             10..285
                     /locus_tag="IYE22_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9508011.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            298..2376
                     /locus_tag="IYE22_002839"
     CDS             298..2376
                     /locus_tag="IYE22_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ9508012.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2536..4422
                     /locus_tag="IYE22_002840"
     CDS             2536..4422
                     /locus_tag="IYE22_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9508013.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4434..5381
                     /locus_tag="IYE22_002841"
     CDS             4434..5381
                     /locus_tag="IYE22_002841"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ9508014.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5401..5928
                     /locus_tag="IYE22_002842"
     CDS             5401..5928
                     /locus_tag="IYE22_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ9508015.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(5991..6644)
                     /locus_tag="IYE22_002843"
     CDS             complement(5991..6644)
                     /locus_tag="IYE22_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ9508016.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            6776..7618
                     /locus_tag="IYE22_002844"
     CDS             6776..7618
                     /locus_tag="IYE22_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9508017.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            7776..>8277
                     /locus_tag="IYE22_002845"
     CDS             7776..>8277
                     /locus_tag="IYE22_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ9508018.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLE"
BASE COUNT         2841 a         1405 c         1746 g         2285 t
ORIGIN      
        1 atctggaaaa tgagagagag ttttgaacaa caaaaaaaac tgttgcatga tcgctatggc
       61 gccctttcga tggatgaccg ccgtcaaata ctttgtaagt tgcgtaaaag aaatatattg
      121 atgtatcgtc agttggaacg tctgaaacat gatctgcttc gtttggagtc aaaacgtgta
      181 cagtgtgaat tagaggggaa ccaaacacag gttgaagtag tcgaaaccaa aattttaaag
      241 aaaaaagaac aatttttaaa aatgcttaca caaaataaaa aataaagggg atggacgatg
      301 gaatttgctg agttagttat tgtatttgca gtaacgatca ccttatcgaa catagctagc
      361 cgcattttgc caatgattcc agctccactg atccaaattt tccttggagt ggtattaggt
      421 ctgacagagt ggggacaatc aatcgatttt gaacctgaat tgttcttagt tatgattatt
      481 gcaccattgt tatttagaga aggagaaaaa gcagatattt cttcgattct caaaaatttc
      541 gggacaattt tgttcttggc atttggagga gtcattttga cccttgtcgg ggtaggtgcg
      601 acgttatcat tcctgttgcc aagtgtccca cttgctgcct gctttgcttt tggagcagcg
      661 ttaggtccga cagatgcggt agcagtcagt tctctttctg gacgagtgaa tatccctaaa
      721 aaggcgatgc atattttgga aggagaaggt cttttgaatg atgcatctgg tgttactgcc
      781 ttccagtttg ctcttggtgc gctgatcacc ggatcttttt cagcagtaaa cgcggggatg
      841 agtctagtag tttctagtat tgggggagct ttgatcggct ttttgcttgt ctggttcaaa
      901 caaaaaataa ttcatttgat cgaaaaagca tcagcacaag atgtcacagg gtatctgctg
      961 atcgaacttt tgcttccttt tctagcctat gtattggcag aggcatttga tgtatctgga
     1021 atcattgctg cagtggcagc aggtattctc caagcttctg gttttaggaa aatctctgtg
     1081 tttgatgcag aattgtcgag cttgtcccac agtacatgga caactattgc ttttacactg
     1141 aatgcattag tatttatatt tttaggaatc gaattgacgc aagtgttctc gccagtatgg
     1201 ggagacggtt tatatccaaa cggtctttta ttagcgatta tcgtattaat ctctgtcatg
     1261 ctttttgtga tcagattcat ttctatcagt cttttttatg tattcaaaga tggcagtaaa
     1321 aagttcaaaa aacagttgaa cgagatcttg atcttaacat ttggcggtgt aaagggaaca
     1381 gtcagtttgg ctacgatttt tattttgcca ttttcaatca ataatatgat gttttaccag
     1441 cggtctttgc ttttgtttct gacggcgggt gtcatcttag tgactttggt catagggatc
     1501 attgtcttgc ctatgctgac ggaaacagag gaagcgaaaa gcacggattt gaatgctttg
     1561 atgattttag aagaggtcgt tgaagttttg cgaaaagaaa taaaggaaat tgatcgtaat
     1621 acaaaagaat ttttagccac tgaagcagtg atagaaaatt atcaagaacg gatacgtgat
     1681 ctgtacttgg aagatttaac cgatgatgaa aagcaagaag tacaagaaat acaagcactg
     1741 attttgtcga tcgaacggga tggattggat gaaagttatc gttcgggcaa gctttcttct
     1801 aatggttatc gtttttactc ccgttttctt tctcgattcg aacattctat cacaagccag
     1861 attttgtctt ttatcggctt ttggttcatt gttgtccgta ggttaatgcg aatcgttctc
     1921 catccgaaaa tgttttggca aagaagatat gctgatcgtc agacatttat tagtgatgaa
     1981 gatatccaag aaatcagaga gacatatcaa aaaaatactc agttgattat tgaaagttta
     2041 gataacttga ctgatattta tgatgaaacg atggttcaat tttttatcca gcaacgcaaa
     2101 cttgaaggaa taaaaatgaa ctcggggaat ttgatttctt cgtggatgat ccagcaagat
     2161 agtctgttta cgaaaaaaat gctaagaggt tattatcttg aaagaaaaat cattgatgaa
     2221 tacgaagttg ctgaaaaaat cacgacgttt tctgcgaaca gctatcgcag gaatatcaat
     2281 ttgttagaat cttatacgat gaacaaacca tctgataact tttctttccg atttgcgttt
     2341 cgagcaaagt caaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta
     2401 taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca
     2461 aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca
     2521 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
     2581 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
     2641 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
     2701 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
     2761 caagcagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
     2821 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
     2881 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
     2941 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
     3001 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
     3061 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
     3121 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
     3181 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
     3241 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
     3301 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
     3361 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
     3421 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
     3481 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     3541 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     3601 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     3661 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     3721 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     3781 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     3841 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     3901 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     3961 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     4021 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     4081 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     4141 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     4201 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
     4261 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
     4321 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
     4381 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
     4441 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
     4501 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
     4561 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
     4621 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
     4681 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
     4741 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
     4801 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
     4861 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
     4921 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
     4981 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
     5041 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
     5101 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
     5161 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
     5221 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
     5281 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
     5341 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
     5401 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
     5461 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
     5521 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
     5581 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
     5641 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
     5701 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
     5761 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
     5821 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
     5881 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
     5941 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
     6001 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
     6061 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
     6121 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
     6181 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
     6241 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
     6301 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
     6361 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
     6421 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
     6481 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
     6541 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
     6601 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
     6661 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
     6721 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
     6781 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
     6841 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
     6901 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
     6961 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
     7021 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
     7081 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
     7141 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
     7201 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
     7261 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
     7321 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
     7381 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
     7441 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
     7501 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
     7561 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
     7621 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
     7681 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
     7741 aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga
     7801 tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta
     7861 taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa
     7921 aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa
     7981 ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga
     8041 ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc
     8101 gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga
     8161 ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca
     8221 attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaag
//
LOCUS       DACIRY010000268         7959 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.121, whole genome shotgun sequence.
ACCESSION   DACIRY010000268 DACIRY010000000
VERSION     DACIRY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.121"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            128..1564
                     /locus_tag="IYE22_002846"
     CDS             128..1564
                     /locus_tag="IYE22_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9508019.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IYE22_002847"
     CDS             1753..4200
                     /locus_tag="IYE22_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ9508020.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IYE22_002848"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IYE22_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ9508021.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IYE22_002849"
     CDS             complement(5690..6793)
                     /locus_tag="IYE22_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9508022.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IYE22_002850"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IYE22_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ9508023.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACIRY010000269         7481 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.128, whole genome shotgun sequence.
ACCESSION   DACIRY010000269 DACIRY010000000
VERSION     DACIRY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.128"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            111..194
                     /locus_tag="IYE22_002851"
     CDS             111..194
                     /locus_tag="IYE22_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ9508024.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="IYE22_002852"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="IYE22_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ9508025.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1226..1486
                     /locus_tag="IYE22_002853"
                     /pseudo
     CDS             1226..1486
                     /locus_tag="IYE22_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1649..2314
                     /locus_tag="IYE22_002854"
                     /pseudo
     CDS             1649..2314
                     /locus_tag="IYE22_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2350..2595
                     /locus_tag="IYE22_002855"
     CDS             2350..2595
                     /locus_tag="IYE22_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ9508026.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2698..3567
                     /locus_tag="IYE22_002856"
     CDS             2698..3567
                     /locus_tag="IYE22_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ9508027.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3548..4282
                     /locus_tag="IYE22_002857"
     CDS             3548..4282
                     /locus_tag="IYE22_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9508028.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4315..5223
                     /locus_tag="IYE22_002858"
     CDS             4315..5223
                     /locus_tag="IYE22_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ9508029.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5220..5762
                     /gene="sat4"
                     /locus_tag="IYE22_002859"
     CDS             5220..5762
                     /gene="sat4"
                     /locus_tag="IYE22_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ9508030.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5855..6649
                     /locus_tag="IYE22_002860"
                     /note="aph(3')-IIIa"
     CDS             5855..6649
                     /locus_tag="IYE22_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ9508031.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6757..6945)
                     /locus_tag="IYE22_002861"
                     /pseudo
     CDS             complement(6757..6945)
                     /locus_tag="IYE22_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6925..7467
                     /locus_tag="IYE22_002862"
     CDS             6925..7467
                     /locus_tag="IYE22_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9508032.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2622 a         1131 c         1566 g         2162 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 aacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1201 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1261 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
     1321 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
     1381 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
     1441 aattggggaa aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc
     1501 caaaagtagc tcctatatga aaaatagagg tctttccatt tagatagtag tggaaagacc
     1561 tctatttatc ttttgaaagt gaatacaaaa ataaagcaca ctctgctata atataggtga
     1621 ttaatccatt tgagagaggt gctttatcat gtatcaaaat tataccataa accaactttg
     1681 tttgcctata gatcttgaaa ttaaattaga agaaaatgat tttgctcatg ccattgtgca
     1741 gtttgttgac tctattcccg atgaagtctt tcttccttat tatcaatcta tgggtagacc
     1801 ccaatatcat cctcgtatgc tactttccat tattctttgt gcctatattc aaggggttta
     1861 ttcgggccgc caaatacaaa atatgctgat agatagtatt cggatgcgct acttatctca
     1921 agagcaattc cctaactttc gtactattaa tcgttttcgc gttcatccga ttatgaacga
     1981 tattctagat cattcatttg ttcaatttcg tgagctactg gttcaaagtg gcttaatcag
     2041 tggcagtgct ctatacattg atggtacaaa aattgaagcg gatgcgaata aatactcttt
     2101 cgtttggaaa aaaagtattt taaagtataa ggatagcctt gatcaaaaag cccttgaaaa
     2161 ctaccaacaa atggtggaaa caaaaatctt acctgagtta atagatgatt taaaggatga
     2221 actttctata gaagagattg aaaggattcg tcaatctttg gaagaaaagg aaaatcaatt
     2281 aattgaagcg attgaccaaa ccgaaacagt agaagggggc tgacccatta ttaataagga
     2341 gtggacaaca tgattgatgt aaatgagcta ttggaagaag ctattagaga aactgaaaat
     2401 ctaaatgatg gcgaagtttt tcttgttaaa gatttattca agggatatgt ggggaatagg
     2461 atacccagaa aggatcgact tctgcttgga acgttatttt taaaccatgt tagcaaaatg
     2521 aatggtaatt taaaggctat tgaaaagact tcatctaatc agcagaggta taaaaagaca
     2581 attgataagt tttaactatc ttttgtgaaa ttaaaaaaat tataaatatg ttgagtagaa
     2641 gcaaaagatt tggcttggaa tttttacagc agtgaatcga taaaaggagc atgcttgatg
     2701 gtagataata ttattaaatc agtagcagag aaattatcct ctctgtctta tatagaaggt
     2761 attgttttag gtggttcacg tgcaaggggc acccatacag aggattcgga tatagatatc
     2821 ggcatctatt acaattcaga atcatttgac ataaatacta ttaatcaatt cgctacaaag
     2881 ctggatgatg agcatagaaa taaccttgtt gtacctcccg gagcatgggg tgattggatt
     2941 aatggcggcg gatggttagt cataaacggg tatcatgtgg atttaatttt acgtgatatt
     3001 aaacgtgtgg aacaaataat gaaagataca gagcacggaa ttgttactgc caattatcag
     3061 actgggcatc cccatggtta tattagtgca atgtatcgag gagaattagc gattagcaaa
     3121 atactatatg ctaagaatga aagcttatgc gaattaaaaa aacaggcaga aacttatccc
     3181 aatgctttgc agaaaagttt agttaacttt tttatgtttg aagcagggtt ctctttaatg
     3241 tttgtaaaag caaattcggg aacagacgat aaatattata ttgcgggtca tgtttttcgt
     3301 atagtttcat gtttaaatca agtgttattt gcatgtaata atgcttattg tatcaacgaa
     3361 aagaaagcta taaaactgct tgaaactttt gaacataaac ctgaaaaata taccgagaag
     3421 gtaaatcata tttttgaagt actcggtatc tcactttttg aatgctacga catgaccgag
     3481 aagctttata atgaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag
     3541 agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat
     3601 gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct
     3661 acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat
     3721 atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct
     3781 cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga
     3841 agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta
     3901 tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa
     3961 tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta
     4021 taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg
     4081 gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct
     4141 aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga
     4201 gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt
     4261 atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga
     4321 tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga acgtattcga
     4381 attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga atttcaggat
     4441 tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga tgactggctt
     4501 aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt attcccacct
     4561 gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat tgatcttacc
     4621 ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat aaaggttcta
     4681 attgataaag attgtagaat taaaagggac atagttccga ctgatataga ttatcatgta
     4741 agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa tgtaacacct
     4801 tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca ttttaatcag
     4861 attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga aacaggcttt
     4921 aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga ggatttgtgg
     4981 gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga agcattattt
     5041 ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc
     5101 tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa aaaatacact
     5161 ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag gcgggaacag
     5221 tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc gaaccatttg
     5281 aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac
     5341 tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg
     5401 cagattgcct tgaatatatt gacaatactg ataagataat atatctttac taccaagacg
     5461 ataaatgcgt cggaaaagtt aaactgcgaa aaaattggaa ccggtacgct tatatagaag
     5521 atatcgccgt atgtaaggat ttcagggggc aaggcatagg cagcgcgctt atcaatatat
     5581 ctatagaatg ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata
     5641 accttatagc ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta
     5701 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     5761 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     5821 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     5881 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     5941 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     6001 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     6061 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     6121 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     6181 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     6241 ttgtccctat acgaatagct tagacagccg attagccgaa ttggattact tactgaataa
     6301 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     6361 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     6421 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     6481 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     6541 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     6601 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagattt
     6661 agatgtctaa aaagctttaa ctacaagctt tttagacatc taatcttttc tgaagtacat
     6721 ccgcaactgt ccatactctg atgttttata tcttttctaa aagttcgcta gatagagttc
     6781 tatattagag atgtaaagag ttttggtgaa gaggtttgtg agtatggctt ttataaccag
     6841 ggtgcaatga gaagcactaa cagcagctag cttaagaacg cataggatat actttttatg
     6901 gaagtcacag aggaggaata aaatttgaac aggattaaca gagtaacctc tattcttatt
     6961 cagctgcaat caaaaaaaat aattcctgcc aaagaaattg cacagcgatt taatataagc
     7021 ttaaggacag tttacagaga tatccggaca cttgaagaag ccggaatacc aattggatct
     7081 gaggccggaa aaggatattt tcttgtagag ggcttcctac ttccgccggt aatgtttaca
     7141 gcggcggaag tgggtgcatt gattacagca gggaaatttt taaattgcca tggagatgaa
     7201 tcatttataa aggattttga ttcagctatg tataaaatca aatctatttt gaagcatggt
     7261 gaaaaaaact atgcacagga gttggagaac agtattaacg tgtacagcac atctggacag
     7321 aaaaatacat tggcggataa cgttattgca gcaattcaga ctgcaatctg caataaaagg
     7381 gtaatatcaa ttcaatatcc tgcatcagga gggcaagaac cggaaagcag aatgatagag
     7441 ccggttctgt tgcaaagttt taaataaaga ataaaatccc t
//
LOCUS       DACIRY010000270         4944 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.164, whole genome shotgun sequence.
ACCESSION   DACIRY010000270 DACIRY010000000
VERSION     DACIRY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.164"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            21..1061
                     /locus_tag="IYE22_002863"
     CDS             21..1061
                     /locus_tag="IYE22_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ9508033.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1159..1347
                     /locus_tag="IYE22_002864"
     CDS             1159..1347
                     /locus_tag="IYE22_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9508034.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1405..1758)
                     /locus_tag="IYE22_002865"
     CDS             complement(1405..1758)
                     /locus_tag="IYE22_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9508035.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2289..2711
                     /locus_tag="IYE22_002866"
     CDS             2289..2711
                     /locus_tag="IYE22_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ9508036.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2708..2938
                     /locus_tag="IYE22_002867"
     CDS             2708..2938
                     /locus_tag="IYE22_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9508037.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3436..3636
                     /locus_tag="IYE22_002868"
     CDS             3436..3636
                     /locus_tag="IYE22_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ9508038.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3663..4856
                     /locus_tag="IYE22_002869"
     CDS             3663..4856
                     /locus_tag="IYE22_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9508039.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         1705 a          798 c          912 g         1529 t
ORIGIN      
        1 aaaaaataaa agttacagaa atgtatactt caataaatgg tgaattatgt aagattgata
       61 gagcttattc tggagaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc
      121 ttggagatac aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac
      181 aaacaactgt tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg
      241 aaatctcaga tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta
      301 tactttcttt cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt
      361 atcatgtgga gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa
      421 atgcagaata taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt
      481 tatctgtatc accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg
      541 gatacttaaa tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac
      601 aaggattata tggttggaat gtgacggatt gtaaaatctg ttttaagtac ggtttatact
      661 atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta ctggagcaag
      721 cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa gtttatgcac
      781 cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca aatatcgtaa
      841 atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct cgatgtattc
      901 aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt ttagcagagc
      961 taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc
     1021 ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta tgttgttata
     1081 taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt gaattaaagt
     1141 ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat ttgtggaaat
     1201 aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt atactgcccc
     1261 aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac aatcaaagag
     1321 ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct tatcggctct
     1381 ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat tgataccttt
     1441 tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt atttttctac
     1501 ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt catcaataga
     1561 tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt gtcctttgtt
     1621 ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt gttcacgagt
     1681 gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa aatcgtattt
     1741 atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct atataaccat
     1801 aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca ctgtggaatt
     1861 tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt acctatacaa
     1921 ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt aatggaggga
     1981 aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt cttatcaaaa
     2041 aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca gattacatag
     2101 atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt ttttggtcac
     2161 ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg
     2221 ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac agaaaggggg
     2281 tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt gactatatct
     2341 gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac agactatcag
     2401 caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt tctactgttg
     2461 atacgtacgt cactgatttc caactattta aattatttgg attaagaatc aatgttgaaa
     2521 gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac attattttac
     2581 ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat ttgaatcgtt
     2641 cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc atgaaggagt
     2701 gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa aggcgacaga
     2761 tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata cctcaaaacg
     2821 ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg atgaaaccct
     2881 acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa ttaaatagtc
     2941 ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt
     3001 attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag ccaaacgttc
     3061 tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct gatgaactaa
     3121 cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa gtaatcttta
     3181 gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata gccatagtcc
     3241 gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc agaagtgaaa
     3301 ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt gtcagtgtat
     3361 tattataaaa ctatttgata taattatagg tgaatattgg agttttccta gcctataatt
     3421 gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct aggatatgga
     3481 acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac aaaagggcat
     3541 atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga agaaattcta
     3601 ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa
     3661 ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc
     3721 taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc actactaaaa
     3781 aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa ttaagtgaga
     3841 ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca tggtataaga
     3901 cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag catttctctt
     3961 attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat tggcaactaa
     4021 aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta ttttcagttg
     4081 ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag attattggca
     4141 atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt gaaaaagtaa
     4201 tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct ctgtggtttt
     4261 tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa gatattgatt
     4321 ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa tcgaattata
     4381 gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat aaatgcacat
     4441 taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca gactttgtaa
     4501 tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt gaacgctatg
     4561 ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct catgcttcct
     4621 tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga cacgaagata
     4681 ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc gaggtagcta
     4741 acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat acttcaccaa
     4801 agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt
     4861 gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttcg gctctataat
     4921 ttatgggact gatgaatcag aatt
//
LOCUS       DACIRY010000271         3221 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.197, whole genome shotgun sequence.
ACCESSION   DACIRY010000271 DACIRY010000000
VERSION     DACIRY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.197"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..884
                     /gene="vanY"
                     /locus_tag="IYE22_002870"
     CDS             <1..884
                     /gene="vanY"
                     /locus_tag="IYE22_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ9508040.1"
                     /translation="LFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLENSGTSENTQ
                     EKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIYMSKEI
                     AQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNSGLSLD
                     VGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLPHSAIM
                     KEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEISGNNID
                     GVIVTVFPGSTHTNSRR"
     gene            1037..1522
                     /gene="vanZ"
                     /locus_tag="IYE22_002871"
     CDS             1037..1522
                     /gene="vanZ"
                     /locus_tag="IYE22_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ9508041.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1809..2138)
                     /locus_tag="IYE22_002872"
     CDS             complement(1809..2138)
                     /locus_tag="IYE22_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9508042.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2443..2772
                     /locus_tag="IYE22_002873"
     CDS             2443..2772
                     /locus_tag="IYE22_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ9508043.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2762..3049
                     /locus_tag="IYE22_002874"
     CDS             2762..3049
                     /locus_tag="IYE22_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ9508044.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
BASE COUNT         1164 a          462 c          558 g         1037 t
ORIGIN      
        1 tgttattctt aatatactta ggttatgact acgttaatga agcactgttt tctcaggaaa
       61 aagtcgaatt tcaaaattat gatcaaaatc ccaaagaaca tttagaaaat agtgggactt
      121 ctgaaaatac ccaagagaaa acaattacag aagaacaggt ttatcaagga aatctgctat
      181 taatcaatag taaatatcct gttcgccaag aaagtgtgaa gtcagatatc gtgaatttat
      241 ctaaacatga cgaattaata aatggatacg ggttgcttga tagtaatatt tatatgtcaa
      301 aagaaatagc acaaaaattt tcagagatgg tcaatgatgc tgtaaagggt ggcgttagtc
      361 attttattat taatagtggc tatcgagact ttgatgagca aagtgtgctt taccaagaaa
      421 tgggggctga gtatgcctta ccagcaggtt atagtgagca taattcaggt ttatcactag
      481 atgtaggatc aagcttgacg aaaatggaac gagcccctga aggaaagtgg atagaagaaa
      541 atgcttggaa atacgggttc attttacgtt atccagagga caaaacagag ttaacaggaa
      601 ttcaatatga accatggcat attcgctatg ttggtttacc acatagtgcg attatgaaag
      661 aaaagaattt cgttctcgag gaatatatgg attacctaaa agaagaaaaa accatttctg
      721 ttagtgtaaa tggggaaaaa tatgagatct tttattatcc tgttactaaa aataccacca
      781 ttcatgtgcc gactaatctt cgttatgaga tatcaggaaa caatatagac ggtgtaattg
      841 tgacagtgtt tcccggatca acacatacta attcaaggag gtaaggatgg cggaatgaaa
      901 ccaacgaaat taatgaacag cattattgta ctagcacttt tggggtaacg ttagcttttt
      961 aatttaaaac ccacgttaac taggacattg ctatactaat gatacaactt aaacaaaaga
     1021 attagaggaa attatattgg gaaaaatatt atctagagga ttgctagctt tatatttagt
     1081 gacactaatc tggttagtgt tattcaaatt acaatacaat attttatcag tatttaatta
     1141 tcatcaaaga agtcttaact tgactccatt tactgctact gggaatttca gagagatgat
     1201 agataatgtt ataatcttta ttccatttgg cttgcttttg aatgtcaatt ttaaagaaat
     1261 cggattttta cctaagtttg cttttgtact ggttttaagt cttacttttg aaataattca
     1321 atttatcttc gctattggag cgacagacat aacagatgta attacaaata ctgttggagg
     1381 ctttcttgga ctgaaattat atggtttaag caataagcat atgaatcaaa aaaaattaga
     1441 cagagttatt atttttgtag gtatactttt gctcgtatta ttgctcgttt accgtaccca
     1501 tttaagaata aattacgtgt aagatgtcta aatcaagcaa tctgatcttt catacacata
     1561 aagatattga atgaattgga ttagatggaa aacgggatgt ggggaaactc gcccgtaggt
     1621 gtgaagtgag gggaaaaccg gtgataaagt aaaaagctta cctaacacta tagtaacaaa
     1681 gaaagcccaa ttatcaattt tagtgctgag gaattggtct ctttaataaa tttccttaac
     1741 gttgtaaatc cgcattttcc tgacggtacc cccaaactaa gatttcatag atagattttc
     1801 tgataacttt aaaaaacacg aatagcggaa aggatgtctt cattcgctaa agagtaataa
     1861 actacttttc catcttgacg tgattttgcc attccatgtt tttttaaaaa acgtaaatgg
     1921 tgtgacgttg tagcgacact agcagtaagt atttctgcta aatcacagac gcacatttcc
     1981 ttataggttt ctaaagcata gaaaatttta attctagaaa aatcgctgaa acatttccct
     2041 aacacaagta agtttgaaaa atctttttga tccaatttat tttttattcc atttactttt
     2101 tctttatgta cgacagtcac ttggcaaact tttttcattc atctcacctc acattcaaac
     2161 gattatttga atgaagtata cctatttgat cactcttttg tcaatctacc ttaattccaa
     2221 tacactatta taagcaattt catgtattga agtaaactta cttgcaagtg cgtttttgat
     2281 tccaagttaa aaacatatta ttattctaca taatttccta atcaaataaa actcctttga
     2341 aaattgttct aaaggagttt tattattttg tatttctact aaaaatagcg tacaatgtaa
     2401 ttactaagta attacgaaat gtaaaataat ggaggaaata atatgattac aaaaacaaga
     2461 aaacaaggaa attctattat gttaacggtc cctaaagaat tcgatgtgcc caatggtgtt
     2521 gaagtagaag ctaagcttgt agagaatggt atcctttatg agtttgtaga gcctaaaaaa
     2581 gaattttttg attttagtga agatattttg gctgatattc tttcagaagg ttatagcaaa
     2641 caagagatct taaaagaatt tagaaataga aaaaatgcgt taacttctgc atttaaatct
     2701 attgctgaag atactgttgc aaattctaaa ccgatgacaa aagaggagtt ggcagcagaa
     2761 attggcttat gagattctac cttctaaaca cgtaataaag tatctaaaaa aattaaaaga
     2821 gaaaacatta aaagaacaat ttttaacgat aatttatgat gagatagcag ttcgtcctca
     2881 ttcgggagaa caaaaaacgg gtgatttatc aggaatttgg gctattggat tcaagtatgc
     2941 aggtacgaca tatcgcgttg catatgaaat taaagataat gcagtgatac ctaacttact
     3001 atgtggaacg catgaaaatt tttatgaaca attaaaaaag attaggtaag aacccaatgt
     3061 ttcaacttga gaggatagac gacactttaa aagataattt ttaacagctg agagttgtaa
     3121 taccctataa ttttaaacca ctaacctata tatttatgaa aaatagaaaa aattaaaacg
     3181 cattttaagc ctctttcaaa ttaaaagctt catccttatg c
//
LOCUS       DACIRY010000272         3102 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.201, whole genome shotgun sequence.
ACCESSION   DACIRY010000272 DACIRY010000000
VERSION     DACIRY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.201"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            23..1963
                     /gene="tet(S)"
                     /locus_tag="IYE22_002875"
     CDS             23..1963
                     /gene="tet(S)"
                     /locus_tag="IYE22_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAQ9508045.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            2051..2218
                     /locus_tag="IYE22_002876"
     CDS             2051..2218
                     /locus_tag="IYE22_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9508046.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(2732..3088)
                     /locus_tag="IYE22_002877"
     CDS             complement(2732..3088)
                     /locus_tag="IYE22_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9508047.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
BASE COUNT         1106 a          491 c          528 g          977 t
ORIGIN      
        1 tttctgcttt ttgagctttt gaatggagga aataaaattg aaaattatta atatcggtat
       61 cttagcacat gttgatgcag gaaaaactac tttgacagaa agcttactat acagtagcgg
      121 agcaattaaa gagttaggaa gtgtagatag cggtacaacg aaaacggata ctatgttttt
      181 ggaacgccag agaggtatta ctattcagac cgcaataaca tcttttcaac gggaaaatgt
      241 taaagtaaat attgtagata ctcctggaca catggatttt ttggcagatg tataccgttc
      301 attatctgtt ttggatggag ctattttgct aatctctgca aaagatggag tacagtcaca
      361 aactcgtata ctattccatg cacttagaaa gatgaacata cctataatat tttttattaa
      421 caaaattgat caaaatggaa taaatttgcc agatgtttat caagatatta aggacaaact
      481 ttctgacgac atcataatta agcagactgt gaatctaaat ttgaaacctt atgtaataga
      541 ttatactgaa ccagaacaat gggagacagt aattgtggga aatgattatt tattagaaaa
      601 atataccatt gggaaaacat tgaatattgc agaacttgaa aaggaggaaa acgaaagaat
      661 tcaaagttgc tccttatatc ctgtttatca cggaagtgca aagaataata ttggaattaa
      721 acaacttata gaggtaatta ctagcaaatt attttcaccc acacaactca attcagataa
      781 actttgtgga aatgttttta aagtagaata ttcagatgat ggtcaacggc ttgtctatgt
      841 acgtctttat agtggaacgc tacatttgcg agactcagtc aatatatcag aaaaggaaaa
      901 aataaaagtt acagaaatgt atacttcaat aaatggagaa ttacgccaga tagataaggc
      961 agagcctggt gagattatta ttttaaaaaa tgagctttta aaactaaata acgtacttgg
     1021 agataaaaaa agattaccac atagagaaat tcttgagaat cctcttccta tgttacaaac
     1081 aacaattgaa ccatgtaaat cagtacaaag agaaaagtta ctagatgcac tttttgaaat
     1141 atccgatagt gatccccttc tacaatatta tgtagataca gtaactcacg aaattgtgct
     1201 atctttttta ggtgaggtcc aaatggaggt aacttgtact ctgattcaag aaaaatatca
     1261 tattgagata gaaacaagaa aaccaactgt catttatatg gaaagaccat taaaaaaatc
     1321 tgaatttacc attgatatcg aagtacctcc aaatcctttc tgggcttcta ttggtttatc
     1381 tgtaacacca cttcctttgg gtagtggcat tcagtatgag agcctggttt ctctaggtta
     1441 tttaaatcaa tcatttcaaa atgcagttat ggaaggtata cgctatgggt gtgaacaagg
     1501 attgtacggt tggaaattaa cagactgtaa gatctgtttt aagtatggtc tatattacag
     1561 ccctgtcagt acgccagcag atttccgaat gcttgcgcct attgtactag agcaggcttt
     1621 tagaaagagt ggtacagagt tattagagcc atatcttagc ttcgaaattt atgtaccaca
     1681 agaatatctt tcgagagcat ataatgatgc ttccaaatat tgtgcaaata ttttaaatac
     1741 taagttaaaa ggtaacgagg tcattctcat tggtgaaatt ccagcccgtt gtattcaaga
     1801 gtatcgaaac agtttaactt tctttacaaa tggacgcagt gtctgtttaa cagagttaaa
     1861 aggttatcag gttactaaca ttaagtctgc tttccaacca cgtcgtccaa ataatagaat
     1921 agacaaagta aggcatatgt ttaataaaat caacttacat tgatagtgaa ctaaagagaa
     1981 atttttaaaa tgaatattga gttgttaatt agaacaggag gatatagata ttgtaaatac
     2041 taggtggata ctgtgcccta tatgtggcaa taagacccgt ttaaaaatga gggaagatac
     2101 tgaattaaaa aatttccctc tattttgtcc taaatgcaga caagaaattt taattgaaat
     2161 aacgaagttt agaataactg taattacaga gccagacgca aagacgcaga gccgataatt
     2221 cgagatatca aaatctcatt ttatcggctt tttcttttct atgcatacca ttgagcttag
     2281 ttcttgatat cctttataag aaaataagtg tactgatttt ttttgcagta gctgtattac
     2341 agtagacatc cctcaagcat aaaaagtata caattaaaat tcaatacttg attattgatg
     2401 aagtacagag ttactcgtca tttcaaaact agtatatcag tcaagttttt cctaaggaac
     2461 aaaaacgaac ttcgctatgg gaatcaaaaa tttttagtcg gatgaagtga tatgcaataa
     2521 atccagaaaa ttttcccaac aacaaaaaat gaactgtact ttgcagcgat aagagtaacc
     2581 aaagaactat ttatcattcg ctctagttcc taagtgtaac aaagataaat tcattttcta
     2641 tttatgatta acaaaaatca gaaaaaattc attacatctt tgtaatcaat ctttcctgat
     2701 agtattaatt aatccattgt gtattattga actagttaaa tttacatttt tctccgtaaa
     2761 ttgcttgaat cactttccca atcgtcacta attcatcttc atccagacaa tccaactgtg
     2821 cttctacttg aagacgtttg gagcttttat gactgttttc ttcccctaaa ataaatccat
     2881 ccaaagaaat atttagcaaa gtcactaaat catatacaac atgaaaactt ggctgttgtc
     2941 ctttattttc aatgttcgtc aaatagcgtg gcgcaatctg gatcattgat cctacctgct
     3001 cacgagtcaa tcctttacta agacgggctt ctttaatcgc aagacccaat ggtttgaaat
     3061 caaaatttga tagagtagtt tgccatattc ttgcacccaa cg
//
LOCUS       DACIRY010000273         2551 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.210, whole genome shotgun sequence.
ACCESSION   DACIRY010000273 DACIRY010000000
VERSION     DACIRY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.210"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..459
                     /locus_tag="IYE22_002878"
     CDS             <1..459
                     /locus_tag="IYE22_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9508048.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR"
                     /locus_tag="IYE22_002879"
     CDS             673..1368
                     /gene="vanR"
                     /locus_tag="IYE22_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ9508049.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="IYE22_002880"
     CDS             1346..2500
                     /locus_tag="IYE22_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ9508050.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          880 a          453 c          535 g          683 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa t
//
LOCUS       DACIRY010000274         2534 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.211, whole genome shotgun sequence.
ACCESSION   DACIRY010000274 DACIRY010000000
VERSION     DACIRY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.211"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            245..1213
                     /locus_tag="IYE22_002881"
     CDS             245..1213
                     /locus_tag="IYE22_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ9508051.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IYE22_002882"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IYE22_002882"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ9508052.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..>2534
                     /locus_tag="IYE22_002883"
     CDS             2243..>2534
                     /locus_tag="IYE22_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ9508053.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYP"
BASE COUNT          747 a          486 c          621 g          680 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat ccca
//
LOCUS       DACIRY010000275         1626 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.230, whole genome shotgun sequence.
ACCESSION   DACIRY010000275 DACIRY010000000
VERSION     DACIRY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.230"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..558
                     /locus_tag="IYE22_002884"
     CDS             <1..558
                     /locus_tag="IYE22_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAQ9508054.1"
                     /translation="PVAATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDS
                     KILIEQAVSGCEVGCAVLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITV
                     PADLSAEERGRIQETAKKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRY
                     PRMMAAAGIALPELIDRLIVLALKG"
     gene            564..1172
                     /locus_tag="IYE22_002885"
     CDS             564..1172
                     /locus_tag="IYE22_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ9508055.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          474 a          314 c          405 g          433 t
ORIGIN      
        1 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
       61 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
      121 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
      181 gtattgggaa acagtgccgc gttagctgtt ggcgaggtgg accaaatcag gctgcagtac
      241 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
      301 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
      361 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
      421 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
      481 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
      541 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
      601 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
      661 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
      721 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
      781 atcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
      841 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
      901 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
      961 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     1021 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     1081 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     1141 ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat
     1201 ataagctaac tctttcggca ggaaacccga cgtatgtaac tggttcttag ggaatttata
     1261 tatagtagat agtattgaag atgtaaggca gagcgatatt gcggtcatta tctgcgtgcg
     1321 ctgcggcaag atagcctgat aataagactg atcgcataga ggggtggtat ttcacaccgc
     1381 ccattgtcaa caggcagttc agcctcgtta aattcagcat gggtatcact tatgaaaatt
     1441 catctacatt ggtgataata gtaaatccag tagggcgaaa taattgactg taatttacgg
     1501 ggcaaaacgg cacaatctca aacgagattg tgccgtttaa ggggaagatt ctagaaatat
     1561 ttcatacttc caactatata gttaaggagg agactgaaaa tgaagaagtt gtttttttta
     1621 ttgtta
//
LOCUS       DACIRY010000276         1521 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V615
            SAMEA5368267-rid9536393.guided.233, whole genome shotgun sequence.
ACCESSION   DACIRY010000276 DACIRY010000000
VERSION     DACIRY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368267
            Sequence Read Archive: ERR3171508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699990.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:59:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,887
            CDSs (total)                      :: 2,858
            Genes (coding)                    :: 2,802
            CDSs (with protein)               :: 2,802
            Genes (RNA)                       :: 29
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 29 of 56
            Pseudo Genes (incomplete)         :: 24 of 56
            Pseudo Genes (internal stop)      :: 13 of 56
            Pseudo Genes (multiple problems)  :: 7 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368267-rid9536393.guided.233"
                     /isolate="V615"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            14..925
                     /locus_tag="IYE22_002886"
     CDS             14..925
                     /locus_tag="IYE22_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ9508056.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1078..>1521
                     /gene="vanZ"
                     /locus_tag="IYE22_002887"
     CDS             1078..>1521
                     /gene="vanZ"
                     /locus_tag="IYE22_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ9508057.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVL"
BASE COUNT          539 a          202 c          290 g          490 t
ORIGIN      
        1 gaggagactg aaaatgaaga agttgttttt tttattgtta ttgttattct taatatactt
       61 aggttatgac tacgttaatg aagcactgtt ttctcaggaa aaagtcgaat ttcaaaatta
      121 tgatcaaaat cccaaagaac atttagaaaa tagtgggact tctgaaaata cccaagagaa
      181 aacaattaca gaagaacagg tttatcaagg aaatctgcta ttaatcaata gtaaatatcc
      241 tgttcgccaa gaaagtgtga agtcagatat cgtgaattta tctaaacatg acgaattaat
      301 aaatggatac gggttgcttg atagtaatat ttatatgtca aaagaaatag cacaaaaatt
      361 ttcagagatg gtcaatgatg ctgtaaaggg tggcgttagt cattttatta ttaatagtgg
      421 ctatcgagac tttgatgagc aaagtgtgct ttaccaagaa atgggggctg agtatgcctt
      481 accagcaggt tatagtgagc ataattcagg tttatcacta gatgtaggat caagcttgac
      541 gaaaatggaa cgagcccctg aaggaaagtg gatagaagaa aatgcttgga aatacgggtt
      601 cattttacgt tatccagagg acaaaacaga gttaacagga attcaatatg aaccatggca
      661 tattcgctat gttggtttac cacatagtgc gattatgaaa gaaaagaatt tcgttctcga
      721 ggaatatatg gattacctaa aagaagaaaa aaccatttct gttagtgtaa atggggaaaa
      781 atatgagatc ttttattatc ctgttactaa aaataccacc attcatgtgc cgactaatct
      841 tcgttatgag atatcaggaa acaatataga cggtgtaatt gtgacagtgt ttcccggatc
      901 aacacatact aattcaagga ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca
      961 gcattattgt actagcactt ttggggtaac gttagctttt taatttaaaa cccacgttaa
     1021 ctaggacatt gctatactaa tgatacaact taaacaaaag aattagagga aattatattg
     1081 ggaaaaatat tatctagagg attgctagct ttatatttag tgacactaat ctggttagtg
     1141 ttattcaaat tacaatacaa tattttatca gtatttaatt atcatcaaag aagtcttaac
     1201 ttgactccat ttactgctac tgggaatttc agagagatga tagataatgt tataatcttt
     1261 attccatttg gcttgctttt gaatgtcaat tttaaagaaa tcggattttt acctaagttt
     1321 gcttttgtac tggttttaag tcttactttt gaaataattc aatttatctt cgctattgga
     1381 gcgacagaca taacagatgt aattacaaat actgttggag gctttcttgg actgaaatta
     1441 tatggtttaa gcaataagca tatgaatcaa aaaaaattag acagagttat tatttttgta
     1501 ggtatacttt tgctcgtatt a
//
